LOCUS       DASPXW010000001         4348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1513791, whole genome shotgun sequence.
ACCESSION   DASPXW010000001 DASPXW010000000
VERSION     DASPXW010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1513791"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(406..1140)
                     /gene="trxA"
                     /locus_tag="VEL04_00005"
     CDS             complement(406..1140)
                     /gene="trxA"
                     /locus_tag="VEL04_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HYT46778.1"
                     /translation="MDISKVNFEREVIEASAAVPVLVDFWAPWCGPCKSLGPVLEKLE
                     REYAGRFKLVKVNSDENQELAAAFNVRSIPAVFAFRNGRAVDQFLGALPESQVRAFIE
                     RQLPSAFEIQLERAEQLIAEGRIDEAEPLLADIPQNFDWDAKLEALRAAIGYARSGGA
                     TEAELEARLAANPADHEARLALARLHAGKRHYRQAMDALLEIVRQDKNWRDGEARKQL
                     LHMFTLVEGEPDLVSEFRRKLATALY"
     gene            1189..1527
                     /gene="fdxA"
                     /locus_tag="VEL04_00010"
     CDS             1189..1527
                     /gene="fdxA"
                     /locus_tag="VEL04_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008528408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HYT46779.1"
                     /translation="MTYVVTEDCIKCKYMDCIEVCPVDCFYEGDNMLVIHPDECIDCG
                     VCEPECPPKAILPDSDGKAEKWLKINAEFAVKWPNITRKGTPPPDADAHKDEPDKFKK
                     YFTPEPGKGE"
     gene            1531..2757
                     /locus_tag="VEL04_00015"
     CDS             1531..2757
                     /locus_tag="VEL04_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_277762492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X catalytic domain-containing protein"
                     /protein_id="HYT46780.1"
                     /translation="MELTSEERAILAGKHGEAARRALEYQIEVGRFWGARRLVPVSNV
                     HMMGDIEVMGDAGLEYLREEARLEARCRVPTTTNARCVDFAHCDKLGQDPAEVAKERE
                     LIQCLRRMDVVTTDTCINYQTVYQPRLGEHVAWGDTGTVIYANSVFGARSNFEAGPAA
                     FAAALTGRTPEYGFHLDEHRRGTFSVELKANLGDLADWGAVGKLVGEAHQDYFAVPVF
                     HGYRRTPTADELKHLGAALASYGSMAMFHFVGVTPEAPTNLNNLEEQFVIGDEEIESV
                     YSSYSLGDGDARLVVFSGPQQSLPEMKRLAELFHERKIKHGAACFVTTNGAVLAQASA
                     LGYAGTLQEAGVTILEGVCFYILQNLSAMRSRNRWTNMVSNSAKIVNIIGAHRFNTIL
                     RRTADCVEIACSGILR"
     gene            2763..3164
                     /locus_tag="VEL04_00020"
     CDS             2763..3164
                     /locus_tag="VEL04_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HYT46781.1"
                     /translation="MSVEKAYGPIVEGEALVSEEGFSPRYDLDRWSGLITRPGHRLEG
                     ASIVDKVCFFPSAKGGIAAGWAFYDIKWKKIAPKAFIFGVTNPVMVQGAVFAGIPITA
                     GWTPHPREVVKTGDWVRVDPGRRLVEVMRAG"
     gene            3243..3749
                     /locus_tag="VEL04_00025"
     CDS             3243..3749
                     /locus_tag="VEL04_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT46782.1"
                     /translation="MKSILALLIAAAIAGCAGMGGRELMPGQSTAAEVEAKWGRAAEK
                     HTHADGETVYYYPQLPWGRVTYAARIGPDERLIAFEQRLTEENIAKVVRGRSTALEVR
                     DLLGPPYQPQTIARMNRDIWTYPMRIAGDPTPKWFLVQLSLDDGVVRETYLMDDPQFV
                     PKDGGRRR"
     gene            complement(3724..>4348)
                     /locus_tag="VEL04_00030"
     CDS             complement(3724..>4348)
                     /locus_tag="VEL04_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYT46783.1"
                     /translation="LYGQGEAPMTITGLPQSEHEKRTHLETCGFPRTGVEVRIFNDRD
                     EELPAGETGEIVTRSDCVMAGYWNHAEATAKALRGGWLHTGDVGSMDAEGFLTIRDRS
                     KDLIISGGANIYPREIEEVLLQHPAVAECSVVGRPHPEWGEEVVAFIVKKQSVEVAEL
                     DKICLDNIARFKRPREYRFVDSLPKNNYGKVLKTELRRQLSAAAPRL"
BASE COUNT          791 a         1390 c         1404 g          763 t
ORIGIN      
        1 accaaaggct tgcgcgcgaa tcggcaacta cgctacgctc cgtcgccgat tcgccttgcc
       61 acatcagagc agctgcaaga catggaaatc tgtaacccat gtcctccgac gtttttgtta
      121 cctatctccc cggccggtca ctttgggatg acgcgcagcg tctagggcga tgccgacaaa
      181 caggagcgcg acgacgacca acagcacccc gactacggtc tccatggcta tagctccttc
      241 aatgattgaa tccaggtcgg acatgaccgt tctagactcc gctgaccgat tcgtcgaaag
      301 gatacacgag agtggcgcaa tgtgatgacc ttggcgcgcg ttagtcagca accgcgactg
      361 cgtcgtatgc gcggggctta ggggcgaggc gggtagtgcg tgatttcagt acagcgccgt
      421 cgccaatttc ctgcggaatt ccgacaccag atccggctcg ccttccacca gcgtgaacat
      481 gtgcagcagc tgcttgcgcg cttcgccgtc gcgccagttt ttgtcctgtc gcacgatctc
      541 gagcagcgcg tccatcgctt gccggtaatg gcgcttgccg gcgtgcaggc gggcgagcgc
      601 gagccgcgcc tcgtggtcgg cgggatttgc cgccaggcgg gcctcgagct cggcctcggt
      661 cgctccgccc gagcgcgcat agccgatcgc cgcgcgcagc gcctcgagct tcgcgtccca
      721 gtcgaaattc tgcgggatat cggcgagcag cggctcggcc tcgtcgatgc ggccctcggc
      781 gatcagctgc tccgcgcgct cgagctggat ctcgaacgcc gagggcagct ggcgctcgat
      841 gaacgcgcgc acctgggact cgggcagggc gccgaggaac tgatcgaccg cccggccgtt
      901 gcggaaggcg aacaccgctg gaatcgagcg cacgttgaac gccgccgcga gctcctggtt
      961 ctcgtccgag ttgactttca ccagcttgaa gcgcccggcg tactcgcgct cgagcttctc
     1021 gaggaccggc ccgagggact tgcacgggcc gcaccagggc gcccagaagt cgaccagcac
     1081 cggcaccgcg gccgaggcct cgatcacttc gcgctcgaaa ttgactttgc ttatgtccat
     1141 gaaccgctca tggtagatat ggggccgcgg gatgcgataa tcaacgccat gacttacgtc
     1201 gtcaccgaag actgcatcaa gtgcaagtac atggactgca tcgaggtctg cccggtggat
     1261 tgcttctacg agggcgacaa catgctcgtc atccaccccg acgaatgcat cgactgcggg
     1321 gtgtgcgagc ccgagtgtcc gccgaaggcg atcctgcccg attcggacgg caaggcggag
     1381 aagtggctca agatcaacgc cgagttcgcc gtgaaatggc cgaacatcac gcgcaagggc
     1441 acgccgcccc ctgacgccga cgcccacaag gacgaacccg acaagttcaa gaagtacttc
     1501 acccccgagc ccggcaaggg cgaatagccg ttggagttga cgagcgagga gcgcgccatc
     1561 ctcgccggca agcacggcga agcggcgcgg cgcgcgctcg agtatcagat cgaagtaggg
     1621 cgcttctggg gcgcgcggcg cctagtgccc gtcagcaacg tccacatgat gggcgacatc
     1681 gaggtgatgg gcgacgccgg gctcgagtac ctgcgcgagg aagcgcgcct cgaagcgcgc
     1741 tgccgcgttc ccaccaccac caacgcgcgc tgcgtcgatt tcgcgcactg cgacaagctc
     1801 ggccaggacc cggcggaggt ggcgaaggag cgcgagctga tccagtgcct gcgccgcatg
     1861 gacgtcgtca ctaccgacac ctgcatcaac taccagacgg tctatcagcc gcgcctcggc
     1921 gagcacgtcg cctggggcga taccggcacg gtgatctacg ccaactcggt gttcggcgcg
     1981 cgctccaact tcgaggccgg cccggcggcc tttgccgctg cgctcacagg ccgtacgccc
     2041 gaatatggtt ttcatctgga cgaacatcgc cgcggtactt tttctgttga attaaaggca
     2101 aatctcggtg acctcgccga ctggggcgcg gtcggcaagc tggtcggcga ggcgcaccag
     2161 gactatttcg cggtgccggt gttccacggc taccgccgca ccccgacagc ggacgagctg
     2221 aagcacctcg gcgccgcgct cgcgagctac ggctcgatgg cgatgttcca cttcgtcggg
     2281 gtgacgcccg aggcgccgac gaatttgaac aatttagaag aacaattcgt cattggggat
     2341 gaagagatcg aatccgtgta ttcgagctac tcactcggcg acggcgacgc ccggctggtg
     2401 gtgttttccg ggccgcagca gtcgctgccc gagatgaagc ggcttgcgga gctttttcat
     2461 gaaagaaaaa tcaagcacgg cgccgcgtgc ttcgttacga cgaacggcgc cgtgcttgct
     2521 caggcgagcg cactcggcta tgcgggaacc ttgcaggaag ctggcgtcac gatcctcgag
     2581 ggcgtgtgct tctacattct gcagaacctg tcggcgatgc gcagccgaaa ccgctggacc
     2641 aatatggtgt ccaactcggc gaagatcgtg aacatcatcg gcgcgcaccg cttcaatacc
     2701 atcctgcggc gcaccgcgga ttgcgtggaa attgcgtgca gcggaatcct tcgatgaaat
     2761 ttatgagcgt ggaaaaagct tacggcccca tcgtcgaagg cgaggcgctg gtgtccgagg
     2821 aaggcttctc gccgcgctac gacctcgacc gctggtcggg cctgatcacc aggccggggc
     2881 accggctcga gggcgcgagc atcgtcgaca aggtgtgctt cttccccagc gccaagggcg
     2941 gcatcgctgc cggatgggcg ttctacgaca tcaagtggaa gaaaatcgcg cccaaagcgt
     3001 tcatcttcgg agtgaccaac cccgtcatgg tgcagggcgc ggtgttcgcc gggatcccga
     3061 tcaccgcggg ctggacgccg cacccgcgcg aggtggtgaa gaccggcgac tgggtgcgcg
     3121 tggacccagg ccgcaggctc gtggaggtga tgcgtgccgg ctgacgcgcg ccgcagcgga
     3181 cgcagaagcg ctcgcgcgca ccgatcggcg cgttgcagtt ccagcatctg cgaaggaacg
     3241 ccatgaaatc gatcctagct cttttaatcg cggcggcgat cgccggctgc gcaggcatgg
     3301 gtggcaggga gctgatgccg ggacaatcca ccgccgctga ggtcgaggcg aaatggggtc
     3361 gcgccgctga aaagcatacg catgcggacg gcgagacggt ttactactat ccgcaactgc
     3421 cgtggggccg tgtcacttat gccgcgcgta tcggacccga cgagcggctg atcgcattcg
     3481 agcagcggct gaccgaggag aacatcgcca aagtggtccg cggccgcagc accgccttgg
     3541 aggtgcgcga cctcctcggc ccgccgtacc agccgcaaac catcgcgcgc atgaaccgcg
     3601 acatctggac ctacccgatg cgcatcgccg gcgacccgac gccgaagtgg ttcctggtgc
     3661 agctctcgct cgacgacggc gtggtgcgcg agacctatct catggacgac ccgcagttcg
     3721 tgcctaaaga cggggggcgg cggcgctgag ctgcctgcgc agctcggtct tcagcacttt
     3781 gccgtagttg ttcttcggca ggctgtcgac gaagcggtac tcgcgcggcc gcttgaaacg
     3841 ggcgatgttg tcgaggcaga ttttgtccag ctcggcgacc tcgacagact gttttttcac
     3901 gatgaaagcc acgacttctt cgccccattc cggatgcggg cgtccgacca ccgagcattc
     3961 ggcgaccgcg ggatgctgca gcaggacttc ctcgatctcg cgcgggtaga tgttggcgcc
     4021 gcccgagatg atcaggtcct tcgagcggtc gcggatggtg aggaagccct cggcgtccat
     4081 gctgccgacg tcgccggtgt gcagccagcc gccacgcagg gccttcgccg tcgcctcggc
     4141 gtggttccag tagccggcca tcacgcagtc ggaacgagtg acgatctcgc cggtttctcc
     4201 cgcaggcagc tcctcgtccc tgtcgttgaa gatcctgacc tccactccgg tgcgcggaaa
     4261 gccgcaggtt tccagatgag tgcgtttttc gtgttctgac tgaggcaagc ccgtaatcgt
     4321 catgggtgcc tcgccctggc cgtagagc
//
LOCUS       DASPXW010000002         3642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_883684, whole genome shotgun sequence.
ACCESSION   DASPXW010000002 DASPXW010000000
VERSION     DASPXW010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883684"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="VEL04_00035"
     CDS             complement(<1..782)
                     /locus_tag="VEL04_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYT46784.1"
                     /translation="MKLRAILGLALAAAALACAGIAAAQPFPSKPVTLICPWPPGGST
                     DVHLRKFAEIAQKYLGQPVVIENKPGASGTLGPSTMAATARADGYTLAQYPISLIRVA
                     HMQKASWDPLKDFSFILGLTGYTFGVVVRSDSPFRSFQDLIEYARANPGRMSFASTGN
                     GTSPHLLMEEVAMKTGVRFLHVPFKGNADSTQALMGGHIMAQSDATGWGRHVDSGAFR
                     LLVTFGEKRTRWDAPTAKELGIDVVSYSPYGIVGPKGMDAQV"
     gene            863..2074
                     /locus_tag="VEL04_00040"
     CDS             863..2074
                     /locus_tag="VEL04_00040"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HYT46785.1"
                     /translation="MLEAYIYDGLRSPIGRHAGKLAPLRPDDLAAEVIRQVVARNQLQ
                     PGDISDVVLGCVNQAGEDCRNVARFSALLAGLPPATPGVTVNRLCASGLQAVVDAARA
                     VTCGEGDLYLAGGVESMSRAPYVMSKSDSPYSRDVKLYDTTIGARFPNASFVKQFGNH
                     SMPETGDHVARDFGITREQADAFALASQQKYATAEKEGFYKGEIHAVALPSKKKDSPR
                     EVVSLDEHPRRDSTLDSLAKLKPLFEGGVVTAGNASGVNDGAAALIVASRKWGEKRGK
                     QPIVKILSAASAGVEPRIMGVGPAYAIPRALERAGLKLAEMDIIEINEAFASQVLACL
                     KLMQVDLQDARVNPNGGAIAIGHPLGASGARLALTVARELQQSGARYAAVSLCIGVGQ
                     GLAVILERVKG"
     gene            2071..3270
                     /locus_tag="VEL04_00045"
     CDS             2071..3270
                     /locus_tag="VEL04_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HYT46786.1"
                     /translation="MNAPLKAKPEARFAWDDPLLLNDQLAQDERMVRDAARAYCQKKL
                     APRILEAFRHEKTDAAIFREMGELGLLGAVIPPEYGGPGLSYVCYGLIAREVERIDSG
                     YRSMLSVQSSLVMVPIQAFGTEAQKKKYLPKLARGESIGCFGLTEPNHGSDPGSMQSR
                     AKQVAGGYSLTGSKTWISNSPIADVFVVWAKDDSGEIRGFILEKNMKGLSAPAIHGKV
                     GLRASITGEVVMDNVFCPDENVFPEVRGLKGPFTCLDSARYGIAWGALGAAEDCMHRA
                     RQYVLERRQFGRPLAANQLVQKKLADMQTEIALGLQAVLRLGRMKDEGSASPEITSIM
                     KRNSCGKALDIARTARDMLGGNGISDEFGVIRHVVNLEVVNTYEGTHDIHALILGRAI
                     TGIQAFN"
     gene            complement(3316..3642)
                     /locus_tag="VEL04_00050"
                     /pseudo
     CDS             complement(3316..3642)
                     /locus_tag="VEL04_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          635 a         1220 c         1210 g          577 t
ORIGIN      
        1 atgacctgcg cgtccatgcc cttcgggccg acgatgccat aaggcgagta cgagacgacg
       61 tcgatgccga gctccttcgc ggtcggcgca tcccagcgcg tgcgcttctc gccgaacgtc
      121 acgaggaggc gaaacgcacc ggaatcgacg tgccggcccc agccggtggc atcggactgc
      181 gccatgatat ggccgcccat cagggcttgc gtcgaatcgg cgttgccctt gaagggcacg
      241 tgcaggaagc gcaccccggt cttcatcgcc acttcttcca tcagcagatg cggcgaggtg
      301 ccgttgccgg tcgaggcgaa ggacatcctg ccggggttcg cccgggcgta ttcgatgaga
      361 tcctggaagc tcctgaaggg cgaatccgac ctcaccacca caccgaaggt atatcccgta
      421 agtccgagga tgaagctgaa gtccttgagc ggatcccacg acgccttctg catgtgggcg
      481 acgcggatca ggctgatggg gtactgcgcg agcgtgtagc cgtcggcgcg ggcggtcgcc
      541 gccatggtgc tcgggccgag cgtgccgctg gcgccgggct tgttctcgat cacgacgggc
      601 tggccgagat atttctgcgc gatctcggcg aacttgcgca ggtgcacgtc ggtcgagccg
      661 ccgggaggcc aggggcagat cagcgtgaca ggcttggacg gaaacggctg cgccgcggcg
      721 attcccgcgc acgcgagagc ggcagcggcg agcgccagtc ccaggatcgc tctgagcttc
      781 atcctcctct tcctccggcg ccgattatag ccacggctgg ccgctagaat agcggtttcc
      841 ccttccatgc tcgacgtttc ccatgctcga agcctacatt tacgacggat tgcgcagccc
      901 gatcggccgg cacgccggca agctcgcgcc gctgcggccc gacgacctgg ccgccgaggt
      961 gatcaggcag gtcgtggcgc gcaaccagct ccagcccggc gacatcagcg acgtggtgct
     1021 cggctgcgtc aaccaggccg gcgaagactg caggaacgtg gcgcgcttct ccgcgctgct
     1081 tgccgggctg ccgcccgcca cgcccggcgt gacggtcaac cgcctgtgcg cgagcggcct
     1141 gcaggcggtg gtcgacgcgg cgcgcgcggt cacctgcggc gaaggcgacc tgtacctcgc
     1201 cggcggggtg gaaagcatga gccgcgcgcc ctacgtcatg agcaagtccg actcgcccta
     1261 cagccgcgac gtgaagctgt acgacaccac cataggcgcg cgcttcccga acgcaagctt
     1321 cgtgaagcag ttcggcaacc actcgatgcc ggagaccggc gaccacgtgg cgagggactt
     1381 cggcatcacc cgcgagcagg cggacgcgtt cgcgctcgcc tcgcagcaga aatacgccac
     1441 cgcggagaag gaaggttttt acaagggaga gatccatgcc gtcgcccttc cctcgaagaa
     1501 gaaagattct ccccgggaag tcgtctccct ggacgaacat ccgcggcgcg attcgacgct
     1561 tgattctctt gcaaaattga aacccctttt tgaaggcggc gtggtcaccg ccggcaacgc
     1621 ttccggcgtc aacgacggcg ccgcagccct catcgtcgcc agcaggaagt ggggagaaaa
     1681 gcgcggcaag cagccgatcg tgaaaattct ttccgctgcc agcgcgggcg tcgagccgcg
     1741 catcatgggc gtcggtccgg cgtatgccat tccgcgcgct ctggagcgcg cgggactgaa
     1801 gctggccgag atggacatca tcgagatcaa cgaggccttc gccagccagg tgctcgcctg
     1861 cctcaagctg atgcaggtcg atctgcagga cgcgcgcgtc aacccgaacg gcggcgccat
     1921 cgccatcggc catccgctcg gcgcctccgg ggcgcgtctc gcgctcaccg tggcgcgcga
     1981 gctgcagcag tccggcgcgc gctatgccgc ggtgtcgctg tgcatcggcg tcggccaggg
     2041 cctcgccgtg atcctcgaaa gggtaaaggg atgaatgcgc cgctgaaagc caagcccgag
     2101 gcccgcttcg cctgggacga tccgctgctg ctgaacgacc agctcgcgca ggacgagcgc
     2161 atggtgcgag acgccgcccg cgcctactgc cagaagaagc ttgcgccgcg catcctcgaa
     2221 gccttcaggc acgagaagac cgatgcggcg atcttccgcg agatgggcga gctcgggctt
     2281 ctcggcgcgg tgatcccgcc cgagtacggc ggcccggggc tcagttacgt ctgctacggc
     2341 ctgatcgcgc gcgaggtcga gcgcatcgac tcgggctacc gctcgatgct gagcgtgcag
     2401 tcctcgctcg tgatggtgcc gatccaggcg ttcggcaccg aggcgcagaa gaagaagtac
     2461 ctgccgaagc tcgccagggg cgaatcgatc ggctgcttcg gcctcaccga gccgaatcac
     2521 ggctcggatc ccggctcgat gcagtcgcgc gcgaaacagg ttgccggcgg ctacagcctc
     2581 acggggagca agacctggat ttcgaattca cccatcgcgg atgtgttcgt cgtctgggcg
     2641 aaagacgatt caggcgagat tcgcggtttt attctcgaaa aaaacatgaa gggcctgtcc
     2701 gcccccgcca tccacggcaa agtcggcttg cgcgcatcga tcaccggcga agtggtgatg
     2761 gacaacgtct tctgccccga cgagaacgtg ttccccgagg tgcgcgggct gaaagggccg
     2821 ttcacctgcc tcgactcggc gcgctacggc atcgcctggg gagcgctcgg cgcggccgaa
     2881 gactgcatgc accgcgcgcg gcaatacgtg ctcgagcgca ggcagttcgg ccgcccgctc
     2941 gccgccaacc agctcgtcca gaaaaagctc gccgacatgc agaccgagat cgcgctcggc
     3001 ctgcaggctg tccttcgcct cgggcgcatg aaggacgaag gcagcgcctc gcccgagatc
     3061 acctcgatca tgaagcgcaa ctcctgcggc aaggcgctcg acatcgcgcg caccgcgcgc
     3121 gacatgctcg gcggcaacgg tatctcggac gagttcggcg tcatccgcca cgtggtcaac
     3181 ctggaagtcg tcaacaccta cgagggcacg cacgacatcc acgccctgat cctcggccgc
     3241 gccatcacgg gcatccaggc cttcaactag atccggcttg cccgccccct gacaaggggg
     3301 cggcgagccg caggcgagcg ggggtttctg ctcgagtcga tcgagcgggc tcatcacgcc
     3361 gcgtccgccc ttgttgagca cgtgggtgta gatcatcgtc gtcgagacgt ccgagtggcc
     3421 gagcagctcc tgcaccgtgc ggatgtcgta gccgccctcc agcagatgcg tggcgaagga
     3481 gtggcgcagt gtgtggcagc tcacgcgctt ggcgatggcg gcggcgtcgg ccgcctcgcg
     3541 gatcgcgcgc tgaggcaccg agtcgtacag atggtgacgg cgcaccacgc cgtcctccgg
     3601 atcggccgag cggtgattgg ccggaaacac gtactgccat ac
//
LOCUS       DASPXW010000003         2297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_883699, whole genome shotgun sequence.
ACCESSION   DASPXW010000003 DASPXW010000000
VERSION     DASPXW010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883699"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VEL04_00055"
     CDS             <1..180
                     /locus_tag="VEL04_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT46787.1"
                     /translation="KRDGSDVEGWLRLVRAYSVLGERDNARAAASDARRALAADPDKV
                     RRLEELVNTLALKAQ"
     gene            200..676
                     /gene="ccmE"
                     /locus_tag="VEL04_00060"
     CDS             200..676
                     /gene="ccmE"
                     /locus_tag="VEL04_00060"
                     /inference="COORDINATES: protein motif:HMM:NF009731.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HYT46788.1"
                     /translation="MTRKQRRLVLIGSGLAVLTVAAALVLSALKDSIVFFNSPTDVAE
                     KHVQPGARIRLGGLVKPGTLARGENLSVRFEVTDGNRAIPVAFTGILPDLFREGQGVV
                     AEGALDAGGLFRADTVLAKHDETYMPKEVADALKKQGHWKDDYDKKVGAAPQGRAQ"
     gene            705..>2297
                     /locus_tag="VEL04_00065"
     CDS             705..>2297
                     /locus_tag="VEL04_00065"
                     /inference="COORDINATES: protein motif:HMM:NF007691.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HYT46789.1"
                     /translation="MIPELGHYALVLALGLALVQAVVPVWGARVRDPVLMGVAVPTAL
                     AQFVFVAIAFAALTACYVTSDFSVLNVAENSHSAMPLIYKFTSVWGNHEGSMLLWVLI
                     LSVFGALVAALGANLPATLKATVLGVQSWIAAAFFLFILLTSNPFLRLAQAPIEGRDL
                     NPILQDIGLAVHPPMLYLGYVGFSITFSFAVAALIDGRIDPAWARWVRPWALAAWMCL
                     TCGIAMGSYWAYYELGWGGWWFWDPVENASFMPWLAGTALLHSAVVMEKRDALKVWTI
                     LLAILAFSLSLIGTFLVRSGVLTSVHSFANDPARGVFILAILVLFIGGGLALFAARAP
                     LLKQGGLFAPVSREGALVLNNLFLTTACATVFVGTLYPLALEALTGEKISVGAPFFNA
                     TFGPLMLPLLVVVPFGPLMAWKRGDLLGVAQRLIAALAVGVIGIAATFVFEGAKPVLA
                     PFAVGLALFVMAGALTDLVERTGLFRVSFGTVLQRAAGLPRSTWGTACAHFGLGLTLL
                     GIVGETGWSAEKIVTLQPGQVVS"
BASE COUNT          293 a          806 c          761 g          437 t
ORIGIN      
        1 aagcgcgacg gctccgatgt ggagggctgg ctccggctcg tgcgcgccta tagtgtgctg
       61 ggcgagcggg acaatgcgcg cgccgccgcc tccgacgccc gccgggcgct cgccgccgac
      121 cccgacaagg tgcgtcgcct cgaggagctg gtgaacacac ttgcgctgaa ggcccaatga
      181 gcacgcggca cggtgatcga tgacccgcaa gcagcgacgc ctcgtgttga tcggctccgg
      241 gctcgccgtg ctcacggtcg ccgcggcgct ggtcttgagc gcgctcaaag actcgatcgt
      301 gttcttcaac tcgccgaccg acgtggcgga gaagcacgtg cagcccggcg cccgcatccg
      361 gctcggcggc ctggtcaagc ccggcaccct tgcgcgcggc gagaacctga gcgtccgctt
      421 cgaggtgacc gacggcaatc gcgccatacc ggtcgccttt accggcatcc tgcccgacct
      481 gttccgcgaa ggtcagggcg ttgtggccga aggcgcgctc gatgcgggcg gcctgttccg
      541 tgccgacacc gtgctcgcca agcacgacga gacctacatg cccaaggagg tcgccgatgc
      601 cctgaagaag caaggacatt ggaaggacga ttacgacaag aaggtgggcg ccgcaccgca
      661 ggggagggcg cagtgatgca ccgcgtcccg gacacgtggc ttcgatgatc cccgaactcg
      721 gccattatgc cctcgttctc gcgctcggcc tggcgctcgt tcaagccgtc gtgccggtct
      781 ggggcgcgcg cgtgcgcgat cctgtcctca tgggcgttgc ggtgccgacc gcgctcgcgc
      841 aattcgtgtt cgtcgcgatc gcgtttgccg cgctcacggc ctgctacgtc acctccgact
      901 tctccgtcct caacgtggcc gaaaactcgc actcggccat gccgctcatc tacaagttca
      961 ccagcgtgtg ggggaaccac gagggctcga tgcttctgtg ggtcctcatc ctcagcgttt
     1021 tcggggcgct ggtggcggcg ctcggcgcca acctgcccgc gacgctgaaa gccaccgtgc
     1081 tcggcgttca gtcgtggatc gcggcggcgt tcttcctgtt catcctgctg acctcgaacc
     1141 cgttcctgcg cctcgcccag gcgccgatcg agggccgcga cctcaatccg atcctgcagg
     1201 atatcggcct cgcggtgcat ccgccgatgc tctatctcgg ctatgtcggc ttctcgatca
     1261 ccttctcctt cgccgtcgcc gcgctgatcg acggccgcat cgatcccgcc tgggcgcgct
     1321 gggtcaggcc atgggcgctc gccgcctgga tgtgcctgac ctgcggcatc gcgatgggct
     1381 cgtactgggc ctattacgag ctcggctggg gcggctggtg gttctgggat ccggtcgaga
     1441 acgcctcctt catgccgtgg ctcgccggca ccgcgctgct gcactcggcg gtggtgatgg
     1501 aaaagcgcga tgcgctgaaa gtctggacca tcctgctcgc catcctcgcc ttctcgctgt
     1561 cgctgatcgg caccttcctg gtgcgttcgg gcgtgctgac ctcggtccac tccttcgcca
     1621 acgatcccgc gcgcggcgtg ttcatcctcg ccatcctggt cctgttcatc ggcggcggct
     1681 tggcgctgtt cgctgcgcgc gcgccgctgt tgaagcaggg cgggttgttc gcgccggtct
     1741 cgcgcgaggg cgcgctcgtg ctcaacaacc tgttcctcac caccgcctgc gcgaccgtgt
     1801 tcgtcggcac gctttacccg ctcgccctgg aggcgctgac gggggagaaa atctcggtcg
     1861 gcgcgccgtt cttcaacgcc accttcggcc ctctcatgct gccgctgctc gtcgtcgtgc
     1921 cgttcgggcc gctgatggcg tggaagcgcg gggaccttct gggagtagcc cagcgcctga
     1981 tcgcggcctt ggcggtcggc gtgatcggca tcgccgccac ctttgtgttc gaaggcgcaa
     2041 aacctgtgct cgcgccgttc gcggtgggct tggcgctgtt cgtgatggcg ggtgcgctca
     2101 ccgacctggt cgagcgcacc ggcctgttcc gcgtctcgtt cggcacggtc ctccaacgcg
     2161 ccgccgggct gccgcgctcg acctggggca ccgcgtgcgc gcatttcggg ctcgggctca
     2221 cccttctcgg catcgtcggc gagaccggct ggagcgcgga aaagatcgtg acgctccagc
     2281 ccggccaggt cgtgtcg
//
LOCUS       DASPXW010000004         2197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_781, whole genome shotgun sequence.
ACCESSION   DASPXW010000004 DASPXW010000000
VERSION     DASPXW010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="VEL04_00070"
     CDS             <1..971
                     /locus_tag="VEL04_00070"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYT46790.1"
                     /translation="SGGAEMIGNYPLHLMILVLLWGYIYTSWALMGRLGMVSFGHGAF
                     MGIGAYTVVMLWNHFGLSPWLGAPAALAFAALVALVIGYPCFRFKIVGHYFALVTLAL
                     SEVVRLLINALRDETGGSLGVTPDSALHEGAAVALGALQFSDKWVWLCIVLAFWLLGL
                     WVWRAVDRSMARLALQAIAEEEEAAASIGIHVTRAKLRITLISALMTCAGGVLYAQYQ
                     LYVNPDTVSGIGISLQMVFGAIAGGMFVMLGPTVGAAFTLLLAEGLRLAFGNRFPALD
                     LTLYGAMLVLFIIYMPKGILGAALDAWARHRGKRAFTAPGASPSRT"
     gene            complement(1042..1443)
                     /locus_tag="VEL04_00075"
     CDS             complement(1042..1443)
                     /locus_tag="VEL04_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004548605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HYT46791.1"
                     /translation="MNPIERIYREWDERNSKRDAEGLLALYAPDATFESPLVPHLMQT
                     ERGILHGHDELWPFFQKLAQRKPSARGHYRAGYLTDGRTLFWEYPRETPGGEQMDFAE
                     VMEIADGLIQRHRVYWGWFGVRVLQDDAYHR"
     gene            1548..2195
                     /locus_tag="VEL04_00080"
     CDS             1548..2195
                     /locus_tag="VEL04_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYT46792.1"
                     /translation="MQHNRISSPASMLADPTRAAIVLALMDGRSRVAKKLALDAGVTP
                     QTASAHLRKLVAANILACEARGRFKHFRIAGAEVAHAVEALSELGAAAGRTLPPAAHE
                     LRFARCCYDHLAGRLGVAITERLPLLGSGVLAELGIDLDALESQRRPLSRCCVDWTER
                     RPHIAGSLGAALLRAYKDRCWLLAIEESRKLVVTPRGRAMFVERFGVDEALFAAS"
BASE COUNT          291 a          765 c          743 g          398 t
ORIGIN      
        1 cctcgggcgg cgctgaaatg atcggcaact atccgctgca tctcatgatc ctggtgctgc
       61 tgtggggcta catctacacc agctgggcgc tgatggggcg cctcgggatg gtgagcttcg
      121 gccacggcgc cttcatgggc atcggcgcct acaccgtggt gatgctgtgg aaccatttcg
      181 gcctgtcgcc gtggctcggc gcgccggccg cgctcgcgtt cgccgcgctg gtcgcgctcg
      241 tcatcggcta tccctgcttc cgcttcaaga tcgtggggca ctacttcgcg ctggtgacgc
      301 tcgcgctctc ggaggtggtg cggctgctca tcaacgcgct gcgcgacgag accggcggct
      361 cgctcggcgt gacacccgac tcggcgctgc acgagggcgc agccgtggcg ctcggcgcgc
      421 tgcagttctc ggacaaatgg gtctggctgt gcatcgtgct cgccttctgg ctgctcggcc
      481 tgtgggtgtg gcgtgcagtg gaccgcagca tggcgcggct cgcgctgcag gcgatagccg
      541 aggaggaaga ggcggccgct tccatcggca ttcacgtcac gcgcgccaag ctgcgcatca
      601 cgctcatctc ggcgctcatg acctgcgccg gcggcgtgct ctacgcccag taccagctct
      661 acgtgaaccc cgacacggtg tccgggatcg gcatcagcct gcagatggtg ttcggcgcca
      721 tcgccggggg catgttcgtc atgctcgggc cgacggtcgg cgcggcgttc acgctgctgc
      781 tcgccgaagg tctgcggctc gcgttcggca accgctttcc cgcgctcgac ctcaccctct
      841 acggcgccat gctggtgctg ttcatcatct acatgccgaa gggcattctc ggcgccgccc
      901 tcgatgcctg ggcgagacac cgcggcaagc gcgcgtttac tgcgccaggc gcttcaccat
      961 cgcgtacatg aactcgcggc cggcgtacag gctgggtatc tcgatgcgct cgtcgaggcc
     1021 gtggatgcgc aagtccgcgg gctagcggtg gtatgcgtcg tcctgcagca ccctgacccc
     1081 gaaccagccc cagtagacgc gatggcgctg gatcaggccg tccgcgattt ccatcacttc
     1141 ggcgaagtcc atctgctcgc cgccgggcgt ttcgcgcggg tattcccaga acagcgtgcg
     1201 gccgtcagtc aggtagccgg cgcggtagtg gccgcgggcg ctgggcttgc gctgcgcgag
     1261 cttctggaag aacggccaca actcgtcgtg gccgtgcagg atgccgcgtt cggtctgcat
     1321 gaggtgcggc acgagcgggc tttcgaacgt cgcgtcgggc gcatacagcg cgagaagacc
     1381 ttcggcgtcg cgcttcgagt tgcgctcgtc ccactcgcgg tagatgcgct cgatcgggtt
     1441 catggcctga cgctagcact gcgccgttcg tgcgagatgc cgatcgcttc gacgctcatc
     1501 gaacgatccg cgtggactcg agcggcgccc ggtgtgagaa ttcttggatg caacacaacc
     1561 ggatttcctc ccctgcctcc atgctcgccg atccgacccg cgcggcgatc gtcctcgcgc
     1621 tgatggacgg ccgcagccgc gtcgccaaga agctcgcact cgacgccggc gtcacgccgc
     1681 agaccgcgag cgcgcatctg aggaagctgg tggcggccaa catcctcgcc tgcgaggcgc
     1741 gcggcaggtt caagcacttc aggatcgccg gtgccgaggt ggcgcacgcc gtcgaggcgc
     1801 tttccgagct cggcgccgct gcgggccgca ccctgccgcc cgccgcgcac gagctgcgct
     1861 ttgcgcgctg ctgctacgac catctcgcgg ggcgactcgg cgtcgcgatc accgagcgcc
     1921 tgccgctgct tggcagcggc gtgctcgccg agctcggcat cgatctcgac gcgctcgaat
     1981 ctcagcgccg gccgctctcg cgctgctgcg tcgactggac cgagcggcgg ccgcacatcg
     2041 ccggatcgct cggcgccgcg cttttgcgcg cctacaagga ccggtgctgg ctgctcgcga
     2101 tcgaggaatc gcgcaagctc gtcgtcacgc cgcgcggccg ggccatgttc gtcgagcgct
     2161 tcggcgtcga cgaagctctc ttcgccgcca gctagcc
//
LOCUS       DASPXW010000005         3665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_127000, whole genome shotgun sequence.
ACCESSION   DASPXW010000005 DASPXW010000000
VERSION     DASPXW010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127000"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..1022
                     /locus_tag="VEL04_00085"
     CDS             129..1022
                     /locus_tag="VEL04_00085"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HYT46793.1"
                     /translation="MTEQPRGVTQMEIDRRVFDLYDEYCHGRIDRREFLKRASALSVA
                     GLAMAQALLPRYALAQTISFTDARIKAQYVIYPSPGGNSGNLRGYLVQPAGKGPFPAV
                     LVIHENRGLNPYIEDVARRVAVEGFLALAPDGLSPAGGYPGNDDEGRELQAKLDQARL
                     RTDMLNSARYLKSHKSSTGKLGVTGFCWGGGTTNFLAVALGADMHAGVPFYGAAPETA
                     GVPKIKAPLLIQYAESDPRINEMWPSYQAALKAAGVPHQAYIYPGTQHGFHNNSTPRY
                     NEAAAKLAWERSIAFFKKNLA"
     gene            980..2896
                     /locus_tag="VEL04_00090"
     CDS             980..2896
                     /locus_tag="VEL04_00090"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYT46794.1"
                     /translation="MGAQHRVLQEKPRLTKRRARRARSASPPPAPAPPAVRPAFSIAA
                     LALLAGALLLYWSALRHPLMFDDYHLSEHALRTYYAQARAWFGLRWLSNASFGWVYAL
                     FGKAMFWQRLANVLLHAATGAVLFGFLSRLFGVVLEDPRSRWLAFFGAALFVLHPVAV
                     YGVAYLMQRSIILATLCSLLALWCVLEWLLRGSPRWCIAAVAAYYLAVFSKEHAIMMP
                     AVAVALAVLVRGASLDLARRAVWPLALFATIAVTVILSSRGVLGRAYEPFASDVVANL
                     GETRSELDSGLAYPISALNQATLYFRYLCTWLLPWPGWMSIDVRTPFPKTLFEWVYMV
                     GFAAWLAYPVAAAWLLFKRGGAGLLGFGLLYPWLFALTEMATVRAQEPFVLYRSYLWM
                     SGLPAIVPMLAGTLAPRWRVAVLSVACLLLVFPARGRLDSFSSQLKLWDDAISKNTNL
                     NAPYVERAHVARGLVHLDAERLEAARVDFERAVELNPRSPDARLARGTLRLRSGALND
                     ALDDLDLAIALDPRYASAYDKRCVVKVGLGRLANAIADCEKAVALEPRNHEAWINTGV
                     VYRSLGRTADAAASYERALELKPENASAHYNYGVLLLESGRRDESMRHHIVVGCEGGI
                     PGACEILKRSSRGR"
     gene            complement(2939..3148)
                     /locus_tag="VEL04_00095"
     CDS             complement(2939..3148)
                     /locus_tag="VEL04_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT46795.1"
                     /translation="MAAIVTTLMYGPVGALLALSSFVALGVSFHSFVTFGDTFNAVVG
                     LLVWWAIGFLPALGYAAFTLGHLHG"
     gene            complement(3330..3407)
                     /locus_tag="VEL04_00100"
     tRNA            complement(3330..3407)
                     /locus_tag="VEL04_00100"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3370..3372),aa:Arg,seq:acg)
     gene            3439..>3665
                     /locus_tag="VEL04_00105"
     CDS             3439..>3665
                     /locus_tag="VEL04_00105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HYT46796.1"
                     /translation="MSFVLVVNICIKPENLDRWMKMALDNAREARKEPGCRQFEVLFD
                     PKEKTKVMLFEIYHDEKAFEAHQQTTHFKKYL"
BASE COUNT          655 a         1172 c         1179 g          659 t
ORIGIN      
        1 gcacgccgac catcaacttg acaggcttga caggatagtt ctgcgcctgc gccgcgaatg
       61 cggcgagagc tagaatgcct gctctcagga tcatggcagc cacattaccg ctaaaggagg
      121 gtcccggcat gactgagcaa ccgcgcggcg tgacgcagat ggaaatcgac cggcgcgtct
      181 tcgatctcta cgacgagtac tgccatggcc gcatcgatcg gcgcgaattc ctgaagcggg
      241 ccagcgcgct cagtgtcgcg gggttggcaa tggcgcaggc gctgttgccg cgctatgcgc
      301 tggcgcaaac gatctccttc accgacgcgc gaatcaaggc gcagtacgtg atttacccgt
      361 ctccgggcgg caactccgga aacttgcgcg gctacctggt ccaacctgct ggcaagggcc
      421 cgtttccggc ggtgctggtc atccacgaaa accgcgggct caacccttac atcgaggatg
      481 tggcgcgccg cgttgcggtg gaaggatttc tcgccctcgc gccggacggt ctatcgccgg
      541 ccggcggcta cccgggcaac gacgacgagg gccgcgagct gcaggccaag ctcgaccagg
      601 ccaggctgcg caccgacatg ctgaacagcg cgcgctatct caagtcgcac aaatcgtcga
      661 ccggcaagct cggcgtgacc ggcttctgct ggggaggcgg cacgacgaat ttcctggcgg
      721 tcgcgctcgg cgccgacatg catgcgggcg tgccgttcta cggcgcggct ccggaaaccg
      781 ccggcgtacc gaagatcaag gcgccgctgc tcattcagta cgcggagagc gacccgcgca
      841 tcaacgagat gtggccaagt tatcaggccg cattgaaagc ggccggcgta cctcatcagg
      901 cgtacattta tccgggcacg cagcacggct tccacaacaa ctccaccccg cgctacaacg
      961 aggcggcggc caagctggcg tgggagcgca gcatcgcgtt cttcaagaaa aacctcgcct
     1021 gacgaagcgc agagcgcggc gcgcgcgttc cgcctcgcct cccccggcgc ccgcgccgcc
     1081 ggctgtacgc ccggcattct cgatcgccgc gctcgcgctg ctcgcgggag cgctcctgct
     1141 ttactggagc gcattgcgcc atcccctgat gttcgacgac taccacctga gcgagcacgc
     1201 gctcaggacg tactacgcgc aggcccgagc gtggttcggc ttgcgttggc tctccaatgc
     1261 gagcttcggg tgggtctacg cgctgttcgg caaggccatg ttctggcagc ggctcgcgaa
     1321 cgtgctgctg cacgcggcga ccggggcggt gctgttcgga tttctttcgc gcttgttcgg
     1381 cgtggtgctc gaggacccgc gctcgaggtg gctggcgttt ttcggggctg ccctgttcgt
     1441 gctccatccg gtggccgtgt acggcgtggc ctatctgatg cagcgctcga tcattctcgc
     1501 gaccctgtgt tcgctgctcg ccctgtggtg cgtgctcgag tggctgttac ggggctcgcc
     1561 cagatggtgc atcgcggccg tcgccgcgta ttacctcgcg gtattctcca aggagcacgc
     1621 catcatgatg cccgcggtcg cggtggcgct ggccgtgctg gtgcgcggcg cgtcgctcga
     1681 cctggcgcgg cgggccgtgt ggcccctggc gctgttcgct acgatcgcgg tgaccgtgat
     1741 cctgagttcc aggggcgtgc taggcagggc ctacgagccc ttcgcatccg atgtggtcgc
     1801 caatctgggc gagacgcgca gcgagctcga ctccggcctc gcctatccga tcagcgcgct
     1861 caatcaggca acgttgtatt tccgctatct ttgcacctgg ctcctgccct ggccgggatg
     1921 gatgtccatc gacgtccgca cgccgtttcc gaagacgcta tttgaatggg tttatatggt
     1981 cgggttcgcg gcgtggctcg cctacccggt cgcggcagcg tggctgctct tcaagcgcgg
     2041 cggcgccgga ttgctcggct tcggcctgct ctatccgtgg ctgttcgcgc tcaccgaaat
     2101 ggcgacggta agggcgcagg agccattcgt gctctaccgc agctacctgt ggatgagcgg
     2161 gcttccggca atcgtaccca tgctggccgg cacgcttgcg ccgcgctggc gtgtcgccgt
     2221 cctgtcggtg gcgtgcctgc tgctcgtatt tccggcgcgc ggccggctgg acagcttctc
     2281 gagccagctc aagctctggg acgacgcgat cagcaagaac accaacctga acgcgccgta
     2341 cgttgaacgc gcgcatgttg cgcgcggctt ggtgcacctc gacgccgagc ggctcgaggc
     2401 ggcacgcgtc gacttcgagc gcgcggtgga gctgaacccg cgctcgccgg acgcgcgcct
     2461 cgcccgtggc acgctgcgcc tgcgcagcgg tgcacttaat gatgcgctcg atgatctcga
     2521 cctcgccata gcgctcgacc cgcgctatgc gtcggcgtac gacaagcgct gcgtggtgaa
     2581 ggtgggtctc ggcaggctgg cgaacgcaat cgccgattgc gagaaagcgg ttgcgctgga
     2641 gccgcgaaac catgaggcgt ggatcaacac cggcgtggtc taccgttcgc tcgggcgcac
     2701 ggccgacgcc gctgcgagct acgagcgcgc cctcgagctg aagcccgaaa atgcatcggc
     2761 tcactacaat tacggcgtgc tgcttctcga gtccgggcgg cgcgacgaat ccatgcgaca
     2821 ccatatcgtc gttggttgcg aaggcggaat cccgggcgcc tgcgaaatcc tgaagcgcag
     2881 cagccgcggt cgatgaatcc ggcgggacat agccataatc caaccggctc cgatcaagtc
     2941 agccgtgcaa atggcccagc gtgaacgccg catagcccaa tgccggcagg aagccgatag
     3001 cccaccacac caatagtccc accactgcgt tgaacgtgtc tccgaacgtc acaaaagagt
     3061 gaaatgaaac acccagcgcg acgaaagacg acaaggcaag caatgccccg acaggcccgt
     3121 acatcaaggt ggttacgata gccgccatca ccgggatttg agaaatggaa gctcgcattt
     3181 tcgtgcctcg ctcctgacat tagaaggacg aagcagggag agggaaaaac cgggtgccga
     3241 tcggcggttc gaacgcagcg cttcgctaac ccttatacac cgatgcggcg aagcatgaaa
     3301 acggccagcg ctcgctgatc catttgattt ggcgcgcccg gcaggatttg aacccacgac
     3361 cccttggttc gtagccaagt actctatcca gtctgagcta cgggcgcgag gcgtgaatta
     3421 tagcaaacgg aggtgtccat gagcttcgta ctggtggtga acatctgcat caagcccgag
     3481 aacctcgatc gctggatgaa gatggcgctc gacaacgcgc gcgaggcgcg caaggagccg
     3541 ggctgcaggc aattcgaggt gctgttcgat cccaaggaaa agacgaaggt gatgctgttc
     3601 gagatctacc acgacgaaaa agcgttcgag gcgcaccagc agacgacgca tttcaagaag
     3661 tatct
//
LOCUS       DASPXW010000006         6403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_505797, whole genome shotgun sequence.
ACCESSION   DASPXW010000006 DASPXW010000000
VERSION     DASPXW010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6403
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505797"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="VEL04_00110"
     CDS             <1..703
                     /locus_tag="VEL04_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT46797.1"
                     /translation="ATLEGLIQRIEATGQLPSGVNLGGNYEPDAALEVMRHLAMYWAS
                     EPPERKHPRHSVKSRLAIAHGFEGVVEALGGAGASLDFNQRSAERWTVENVSAGGIGA
                     IVPQIKGDWLRIGTLLAIQPDGGTNWVVGMVRRVNRVSKQEARVGIQTLSRAPALAKF
                     ALRGAGDQVGVLLPSPVLGSGDTSIALRAGSYTRGQNLETEMDGRQHVYMAQNVAERG
                     DDYEVVRFRQMVREA"
     gene            complement(690..1325)
                     /locus_tag="VEL04_00115"
     CDS             complement(690..1325)
                     /locus_tag="VEL04_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HYT46798.1"
                     /translation="MEAIEIETGPKPAAAVIWLHGLGADGHDFEPVVPELRLPKPVRF
                     VFPHAPVRPVTINQGMRMRAWYDIFQFGGGPEDEKGIRESQGLIEALIKQQPVPARAI
                     VLAGFSQGGAIVLQTALRCPERLAGVIALSSYLPLAGKLQAEKSPANQDLPVFMAHGQ
                     HDDLIPLSRAQKSRELLVDAGYKVEWHDYPMPHSVCGEEIADIASFLVRLL"
     gene            1372..2328
                     /locus_tag="VEL04_00120"
     CDS             1372..2328
                     /locus_tag="VEL04_00120"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYT46799.1"
                     /translation="MKRLVAAAVLLLLSAAACGQVFPTRPVRLVVGFTPGGGVDINAR
                     LLAAKLSELFGQQVIVDNKPGAGTNIANEHVAKAAPDGYTLLFTSPAFAVNMSLHKNP
                     PYDAIRDFSPVSVFSESTNLLVVPASLPAKSVQELIALARQRPGALNYSSAGAGTTQH
                     LAGELFKLRTATNIVHIPYKGSAPSLAALIAGEVQMSFANPLAIGQHVKTGRLRALAV
                     ARARRSELVPEVPTMRQAGVEGVELPLWFGLLAPAGTPRDIVLVLAGAVAKVAHSPEM
                     RKKLVEQGAEPVGSSPEEFARQLRDEVARWAEVVKVSGARAD"
     gene            complement(2325..3470)
                     /locus_tag="VEL04_00125"
     CDS             complement(2325..3470)
                     /locus_tag="VEL04_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HYT46800.1"
                     /translation="MDFRLTPEQQQFRDSVLRFARNELAEKALQRAHTADYPWDVAQR
                     MASQGLLGITIAEADGGQGGTLMDAVLAIEAVASVCPRSADVVQAGNFGPIRVLAEYG
                     SDSQKKRFLKKLLAGEAVIAVGMTEPGAGSAVTDLATRATRDGKGWRINGSKIFTTHS
                     AYADLILAYVRFGPGVAGIGSVLIETKAQGVKQGRRSQFMSGEEWAELSFEEVLVPED
                     MVLLGEGGFKKQIAGFNIERIGNAARSAALGRYAFEAARAWALERRQFGRLLCEFQGL
                     QWKFADMKLELDAAQLLLYRAAANADRGLPSPEDTAIAKAYCNQAGFEVASEALQVMG
                     ATGYSRETLVEYCVRRCRGWMIAGGSVEILKNRIAEAIFNRSFSQRP"
     gene            complement(3473..5437)
                     /locus_tag="VEL04_00130"
     CDS             complement(3473..5437)
                     /locus_tag="VEL04_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HYT46801.1"
                     /translation="MAAGSRSEARLTQALFAPRAVALIGASGDPAKNTARPQRFLRKH
                     GYAGRIFPVNASRAEVLGERCYRNLGEIDAEIDHAFIMIEAVEQALEDCARRGVPVAS
                     VYSDGFADAGPEGARRQARLVERARSLGVRLLGPNSMGVVNLPGRLALTVNAVLEMDA
                     PPAGTTSLVSQSGTMLGTLLSRGAARGLGFAKLVSVGNEADLGVGELVALLAEDPETR
                     VILLFLETVRNSAQLAAAARKAHAAGKPVVAYKLGRSALGERLARSHTGALAGPDRAL
                     DAFFRDCGILRVDMLETLLEIAPLVAGRKPPELKRTPRVAVVTTTGGGAATVVDRLGL
                     HGIECIAPDPESPIVDLTMTATSERYAKTLRELLAWPACDAVLAAVGSSAQFHPELAV
                     EPIVAAQAGEKPLAAFLTPHAERSLALLAARGIAAFRTPEACADAFAAYFAWRRPRQR
                     PSFDAPQPSKDAFELFASLGIPVVEQQIARAPDFAHSIPYPVALKLPGVDHKTETGGV
                     ALNIVDRAAFEVQCKKMNAKELLVQKMVRGLAEAIVGYRDDPVAGPIVLLGAGGTLAE
                     LYEDFAIRLAPVTADEAQEMIAEVKGLAPIRGYRNLPRGDVSALARAVSAFSTISLLK
                     DRPISEAEINPLIVEAGGVAAVDGLVVPKE"
     gene            complement(5404..>6403)
                     /locus_tag="VEL04_00135"
     CDS             complement(5404..>6403)
                     /locus_tag="VEL04_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HYT46802.1"
                     /translation="AQGSSGAQFLRAVVVGYEISTRIGAAMGRAHYRYWHNTGTIGCF
                     GAAAAGAALLQLDQEKFSHALATVATFSAGLQQAFRKDSMSKPLHAGRAAEAGVTAAL
                     AAAEGVTGSLDVIEGEAGYGRAMGYGDAGAPDWSRALAGLGREFNITRMTFKNHACCG
                     HTFAAIDGALQLKRKMNLRAAEIERVRVGTYRAGLEVAHYEEPRTPAEARFSMKYVVA
                     TALTHGSVRLAAFAPPRLKDHETLQLMKRIDVVLDPELDATFPRQRAARVAIEARGGR
                     HEELLQPTRKGDPDLPLTDAELEDKFLELAAPVLGEAPARKLLERLWRLEAEAKLG"
BASE COUNT          997 a         2190 c         2190 g         1026 t
ORIGIN      
        1 cgccacgctc gaggggctga tccagcgcat cgaggcgacg ggacagctgc cctccggcgt
       61 gaatctcggc ggcaactacg agcccgacgc ggcgctcgaa gtgatgcgcc atctcgccat
      121 gtactgggcg tccgagccgc ccgagcgcaa gcacccgcgg cacagcgtga aatcgcgcct
      181 cgcgatcgcg cacggcttcg aaggcgtggt cgaggcgctc ggcggcgccg gcgcatcgct
      241 cgatttcaac cagcgctccg ccgagaggtg gaccgtcgag aatgtcagcg ccggcggcat
      301 cggcgcgatc gtgccgcaga tcaaaggcga ctggctgcga atcggcacgc tgctcgcgat
      361 acagcccgac ggcggcacga actgggtggt gggcatggtc cgccgcgtca accgggtctc
      421 gaagcaggag gcgcgcgtcg gcatccagac cctgtcgcgc gcgcccgcgc tggcgaaatt
      481 cgcgctgcgc ggcgccggcg accaggtcgg cgtgctgctg ccctcgccgg tgctcggctc
      541 gggcgatacc tcgatcgcgc tgcgcgccgg cagctacacc cgcggacaaa acctcgagac
      601 cgagatggac gggcgccagc acgtgtacat ggcgcagaac gtcgccgaac gcggcgacga
      661 ttacgaggtg gtccgattca ggcagatggt cagagaagcc tgaccaggaa gcttgcgatg
      721 tcggcgattt cctcgccgca caccgagtgc ggcatcgggt agtcgtgcca ctcgaccttg
      781 tagcccgcat ccacgagcag ctctcttgat ttctgcgcgc ggctgagcgg gatgaggtcg
      841 tcgtgctggc cgtgcgccat gaagaccggc agatcctggt tcgccgggct tttttccgcc
      901 tgcagctttc cggcaagagg cagatagctc gagagcgcga tcacgcctgc gaggcgctcg
      961 gggcagcgca atgccgtctg cagcacgatc gcgccgccct gcgagaaccc ggcgaggacg
     1021 atggccctgg ccggaacagg ctgttgcttg atcagggctt cgatcaggcc ctgcgattcc
     1081 cggatgccct tttcgtcctc cgggccgccg ccgaactgga agatgtcgta ccaggcgcgc
     1141 atgcgcatgc cctggttgat ggtcaccggg cgcaccggcg cgtgaggaaa cacgaagcgg
     1201 accggcttcg gcagcctgag ctcggggacc accggctcga agtcgtgccc gtccgctccc
     1261 aggccgtgca gccagatcac cgccgcggcg ggcttcggac cggtctcgat ctcgatcgct
     1321 tccatgacgg cagaagctta acgcccgagg ggccgctaaa gtcgtagagc gatgaaacgc
     1381 ctcgttgccg ccgcagtgct gctgcttctc agcgccgcgg catgcggaca ggttttcccg
     1441 acccggccgg tacggctggt ggtcggcttc acccccggag gcggcgtcga catcaatgcc
     1501 cggttgctgg cggcgaagct gagcgagctt ttcggccagc aggtgatcgt cgacaacaag
     1561 cccggcgccg ggaccaatat cgcgaacgag cacgtcgcga aggcggcgcc ggacgggtac
     1621 acgctgcttt tcaccagccc cgcgttcgcg gtgaacatgt cgctgcacaa gaatccgcct
     1681 tacgacgcga tccgggattt ttcccctgtg tcggtgttct ccgagagcac caacctgctg
     1741 gtagtgccgg catcgcttcc cgccaaatcg gtgcaggagc tcatcgccct ggcgcgccag
     1801 cggccgggag cgctcaacta ttcttcggcc ggcgccggga ccacgcagca tcttgcaggc
     1861 gagctgttca agctgcgcac cgccacgaac atcgttcaca tcccttacaa gggcagcgcg
     1921 ccctcgctcg ccgcgctgat cgccggggag gtgcagatga gcttcgccaa cccgctggcg
     1981 attggccagc acgtgaagac cggccgcctg cgggcgcttg cggtggcgcg cgcaaggcgc
     2041 tcggagctgg tcccggaggt gccgacgatg aggcaggcgg gcgtcgaggg cgtcgagctg
     2101 ccgctgtggt tcggcctgct tgcgccggcc ggcacgccgc gcgacatcgt gctggtgctt
     2161 gccggtgcgg tcgcgaaagt ggcgcattcg cccgagatgc gcaaaaagct ggtcgagcag
     2221 ggcgccgagc cggtcggcag ctcccctgag gagttcgccc ggcagctgcg cgacgaggtg
     2281 gcgcgctggg ccgaggtggt gaaggtctcc ggcgcgcgcg ccgactaggg ccgctgcgaa
     2341 aagctgcggt tgaatatcgc ttccgcgatg cggtttttca ggatctccac cgagccgccg
     2401 gcgatcatcc agccgcggca gcgccgcacg cagtactcga ccagcgtctc gcggctgtag
     2461 ccggtcgcgc ccatcacctg cagggcctcg ctcgcgacct cgaaccccgc ctggttgcag
     2521 taggccttgg cgatcgcggt gtcctcgggc gacggcaggc cgcggtcggc gttcgccgcc
     2581 gcgcggtaga gcagcagctg cgccgcatcg agctcgagct tcatgtcggc gaacttccat
     2641 tgcagtccct ggaactcgca cagcagccgc ccgaactgcc tgcgctcgag cgcccaggcg
     2701 cgagccgcct cgaacgcgta gcgcccgagc gcagccgagc gcgcggcgtt gccgatgcgc
     2761 tcgatattga agccggcgat ctgcttcttg aagccgcctt cgcccagcag caccatgtcc
     2821 tcgggcacca gaacttcctc gaagctgagc tcggcccact cctcgccgga catgaattgc
     2881 gagcgtcgtc cttgcttcac cccttgggct ttggtttcga tcaaaacaga accgatgcca
     2941 gccacacccg ggccgaagcg cacgtaagcg aggatcaggt cggcatacgc cgagtgcgtg
     3001 gtgaagatct tcgagccgtt gatgcgccag cccttgccgt cgcgcgtcgc gcgcgtcgcg
     3061 agatcggtca ccgccgagcc cgccccgggc tccgtcatgc cgacggcgat cactgcctct
     3121 cccgccagca gtttttttaa aaatcgtttt ttctgagaat cagaaccgta ttccgcgagc
     3181 acgcggatgg gaccgaaatt gcccgcctgc accacgtcgg cgctgcgcgg gcacaccgag
     3241 gccaccgcct cgatcgcgag cacggcatcc atcagcgtgc cgccctggcc gccgtcggcc
     3301 tcggcgatgg tgatgccgag aagcccctgg ctcgccatgc gctgcgccac atcccagggg
     3361 tagtcggcgg tgtgcgcacg ctgcagtgct ttttccgcca attcatttct tgcaaatcgc
     3421 agcaccgagt cgcggaactg ctgctgctcg ggcgtgagtc tgaaatccat ggtcactcct
     3481 tcggcacgac caggccgtct accgcggcca cgccgccggc ttcgacgatc agcggattga
     3541 tctccgcctc cgaaatgggt ctgtctttca gtagagaaat tgttgaaaaa gccgacacgg
     3601 ccctggccag cgcgctcacg tcgccgcgcg gcaggttgcg gtagccgcgg atcggcgcca
     3661 gtcctttcac ctcggcgatc atttcctgcg cctcgtccgc ggtgaccgga gcgaggcgga
     3721 tggcaaaatc ttcgtagagc tcggcgagcg tgccgccggc gcccagcagc acgatcggcc
     3781 cggccaccgg gtcgtcgcgg tagccgacaa tcgcctcggc gagtccgcgg accattttct
     3841 gcaccagcaa ttctttcgcg ttcatttttt tgcactgcac ttcgaaagct gcacgatcca
     3901 cgatattgag ggcgacgccg ccagtctcgg tcttgtgatc cacacccggc agctttagcg
     3961 caacgggata ggggatcgag tgcgcgaaat ccggcgcgcg agcgatctgc tgctcgacga
     4021 ccggaatgcc gagcgaagcg aacagctcga atgcgtcctt gctgggctgt ggagcatcaa
     4081 atgacgggcg ctgtcgcggc ctgcgccatg cgaaataggc ggcgaaggcg tcggcgcagg
     4141 cctcgggagt gcggaacgcg gcaatgccgc gcgcggcgag cagcgccagc gagcgctcgg
     4201 cgtgcggcgt gaggaaggcg gcgagcggct tttcgccggc ctgcgccgcg acgatcggct
     4261 ccacggcgag ctcgggatgg aactgggcgg acgagccgac cgccgcgagc acggcatcgc
     4321 atgcgggcca cgccaggagc tcgcgcagcg tttttgcata gcgctccgaa gtcgcggtca
     4381 tggtgagatc gacgatcggc gattccgggt cgggcgcgat gcattcgatg ccgtgcaggc
     4441 cgagccggtc gacgacggtg gccgcgccgc cgccggtggt cgtcaccacg gccacgcggg
     4501 gcgtgcgctt cagctcggga ggcttgcgcc cggcgacgag cggcgcaatc tcgagcagcg
     4561 tctcgagcat gtcgacgcgc aggatgccgc agtcgcggaa gaaggcgtcg agcgcgcggt
     4621 ctgggccggc gagggcgccg gtgtgcgagc gcgcgagccg ctcaccgagc gccgagcggc
     4681 cgagcttgta ggcgactacc ggcttgccgg cggcgtgcgc cttgcgcgcc gcggcggcga
     4741 gctgcgcgga attgcgtacg gtctcgagaa aaagcaggat gacgcgcgtt tccggatcct
     4801 cggcgagaag ggcgaccagc tcgcccacgc cgaggtcggc ctcgttgccc accgacacga
     4861 gcttggcgaa ccccaggccg cgcgccgcgc cccgcgagag cagcgtcccc agcatggtgc
     4921 cgctttgcga caccaggctc gtcgtgcccg caggcggtgc gtccatctcc agcaccgcat
     4981 tgaccgtcag cgcgagtcgc cccggaaggt tgacgacgcc catgctgttc ggtcccagca
     5041 ggcgcacgcc gaggctgcgg gcgcgctcga cgagccgcgc ctggcgcctg gcgccttcgg
     5101 gcccggcgtc ggcgaagccg tcgctgtaga cgctcgccac cggcacgccg cgccgcgcgc
     5161 agtcctcgag cgcctgctcg acggcctcga tcatgatgaa ggcgtggtcg atctcggcgt
     5221 caatttcgcc caggtttcta taacagcgct cgccaagcac ttcggcgcgc gacgcattga
     5281 ccggaaagat ccgcccggca tagccatgct ttctgagaaa gcgctgcggc cgcgcggtat
     5341 tcttcgccgg gtcgccggaa gcgccgatga gcgcgacggc gcgcggcgcg aacagcgctt
     5401 gcgtcagccg agcttcgctt cggcttccag ccgccacagg cgctcgagaa gtttgcgcgc
     5461 cggggcctcg ccgagcaccg gagcagccag ctcgagaaac ttgtcttcaa gctcggcatc
     5521 ggtgagcggc aggtcggggt cgcccttgcg cgtgggctgc agcagttcct cgtgccggcc
     5581 gccgcgcgcc tcgatcgcca cccgcgccgc gcgctggcgc gggaacgtgg cgtcgagctc
     5641 cggatcgagg accacatcga ttcgcttcat cagttgaaga gtttcatggt ccttgaggcg
     5701 gggtggcgcg aaggccgcca gccgcacgct gccgtgcgtc agcgcggtgg cgaccacgta
     5761 cttcatcgag aagcgcgcct ccgccggcgt gcgcggctcc tcgtaatgcg ccacctcgag
     5821 gcccgcgcgg taggtgccga ccctgacgcg ctcgatttca gcggcgcgca gattcatttt
     5881 ccttttcagc tgcagggcgc cgtcgatggc cgcgaaggta tgcccgcagc aggcgtggtt
     5941 cttgaaagtc atgcgcgtga tgttgaattc gcggcccagg cctgcaagcg cccggctcca
     6001 gtccggcgcg ccggcgtcgc cgtagcccat ggcgcggccg tagcccgcct cgccctcgat
     6061 gacgtcgagc gagccggtca cgccctcggc cgcggcgagc gcagcggtga ccccggcctc
     6121 cgcggcgcgc ccggcgtgca gcggcttcga catcgagtcc ttgcggaagg cctgctgcag
     6181 ccccgccgag aaggtcgcta ccgtcgccag agcgtgtgaa aatttttcct ggtccagctg
     6241 caagagtgcg gcgccggccg cggccgcgcc gaagcagccg atggtgccgg tgttgtgcca
     6301 gtagcggtaa tgcgcccgcc ccatcgcggc gccgatgcgc gtcgagatct cgtagccgac
     6361 gacgactgcg cgaagaaatt gtgctccgct ggagccttgc gcc
//
LOCUS       DASPXW010000007         3789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_379637, whole genome shotgun sequence.
ACCESSION   DASPXW010000007 DASPXW010000000
VERSION     DASPXW010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379637"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VEL04_00140"
     CDS             complement(<1..280)
                     /locus_tag="VEL04_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT46803.1"
                     /translation="MKRVVLCLAALLSAPFAQAAGYDLESFRLALIDDPVRVEFQGAA
                     EPGKEKFRQAVQFIGASKGWSIANETDGRMELTRTVRGQHFVKVELTYD"
     gene            complement(300..1439)
                     /locus_tag="VEL04_00145"
     CDS             complement(300..1439)
                     /locus_tag="VEL04_00145"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYT46804.1"
                     /translation="MEFSSSPEQERIREAIARLCERFGDDYWLTRDREGGFPVDFHQA
                     FARDGWLGIAMPEAYGGAGLGISSAAVMMQAIAESGAGFSGASAVHMNIFGLNPVVVF
                     GNDEQKRRMLPPLIAGKERACFAVTEPNTGLNTKKISVQAKRHAKHYLISGQKVWIST
                     AQVAEKVLLLARASAGLTLFYTDLDRRHVEVREIEKMGRHAVDSNQVFFDGLPVPEAD
                     RIGEEGKGFEYILHGMNPERILIAAELVGLGRCAVSRAAAYAKQRIVFDRPIGQNQAI
                     QHPLAANWMALEAANLMACKAAWLYDCGKPCGAEANAAKYLAGEACFDACQQAVMTLG
                     GYGYAAEYHVERYLRESLVGRIAPITPQLILCYIAERVLGLPKSY"
     gene            complement(1417..2547)
                     /locus_tag="VEL04_00150"
     CDS             complement(1417..2547)
                     /locus_tag="VEL04_00150"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HYT46805.1"
                     /translation="MSGAWLPLLGIRVVDFSMFVPGPFASAILADLGAAVVKVEPPRG
                     DPGRAYVPVQFDTENRNKRSLAVDLKQPDSAAVVEKLVKQADIVIEGFRPGVAARLGI
                     DYPNLKKHNAKIIYCSISGYGQTGPWRERPGHDVNYVAAAGGLAFAGQWLKPPARSSL
                     PIADMSGGSFAAIAVLSALHERERSGKGAYLDLSLFEAAFFLAAMRHSLDAQVDPRAH
                     IFPVNDVFETADGKRLTLGILEEHFWQSFVRCVPGFDAELFSTDAKRRANGDALSGIL
                     EKIFKKKTAAEWLELLDANDVPVDLCLTPAQSADTAQLKAREAVAGGFALFPVWADGR
                     RGGELRLGVPRLGEHSREILVELGFDDAQIADLVRHGVLVQS"
     gene            complement(2544..3254)
                     /locus_tag="VEL04_00155"
     CDS             complement(2544..3254)
                     /locus_tag="VEL04_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYT46806.1"
                     /translation="MKLLEGDTALVTGAARGIGRGIAAALEEEGARVLRADIEKADVV
                     CDLSSAEGARRLAQEAIRLLGKVSVFVHCASPRRQETQTVLATTFEQWREMLAVNLDA
                     AFVLTQVLGLHMKKERIRGRMLLITSLHAESPRNLPHYSAAKAGETMLVKELARGLGP
                     HGIRVNAIAPGAIPGGGFEAPAGMVEKIPLGRFGTPADVAAMAVAVLSERFGAYVTGA
                     SMVVDGGMALCNWIEPPQ"
     gene            complement(3254..3661)
                     /locus_tag="VEL04_00160"
     CDS             complement(3254..3661)
                     /locus_tag="VEL04_00160"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HYT46807.1"
                     /translation="MKSTLKPGLSFSKSILVDEARCIGFMGKEGMVYATPRMVSDVEY
                     ACRDFLLEHLDAGEDSVGAHVSIDHLAATPLGLKVTIEARVTEVDRRKVVFDFSVKDP
                     VEECGRGRHVRFVVETAKTRERLAAKRAKAGLS"
BASE COUNT          632 a         1291 c         1219 g          647 t
ORIGIN      
        1 ggtcgtaggt cagctcgact ttcacgaagt gctggcctct caccgtgcgc gtcaattcca
       61 tgcgcccgtc ggtttcattg gcgatgctcc agcctttcga cgccccgatg aactggaccg
      121 cctggcggaa tttctccttg cccggttcgg cggcgccctg aaactccacc cggaccggat
      181 cgtcgatcag cgccaggcgg aaactttcga gatcgtagcc ggcggcctgc gcaaagggcg
      241 ccgaaagcag ggctgcgagg cagagcacga ctcgtttcat gttctctccg gatgcatcac
      301 tagtaggatt tcggcagccc gagcacccgc tcggcaatgt agcaaaggat cagctgcggc
      361 gtaatcggcg cgatgcggcc gacgagcgat tcgcgcaggt agcgctcgac gtggtactcg
      421 gccgcgtagc cgtagccgcc gagggtcatc accgcctgct ggcaggcgtc gaagcaggct
      481 tcgccggcga gatatttcgc ggcgttcgct tccgcgccgc agggcttgcc gcagtcgtag
      541 agccaggccg ccttgcacgc catcaggttc gccgcctcga gcgccatcca gttggcggcg
      601 agcggatgct ggatcgcctg gttctggccg atggggcgat cgaagacgat gcgctgcttc
      661 gcgtacgcgg cggcgcggct gaccgcgcag cggcccaggc ccaccagctc ggcggcgatg
      721 aggatgcgct cgggattcat gccgtgcagg atgtactcga agcccttgcc ttcctcgccg
      781 atgcggtccg cctcgggcac cggcaggccg tcgaagaaca cctggttcga atcgaccgcg
      841 tgccggccca tcttctcgat ctcgcgcacc tcgacgtgcc tgcggtcgag gtcggtgtag
      901 aacagcgtca ggcccgcgct ggcgcgcgcc aggagcagca ccttttcagc gacctgcgct
      961 gtcgagatcc acactttctg cccggaaatc aggtagtgct ttgcatgcct ttttgcctga
     1021 acggagattt ttttcgtgtt cagacccgtg ttcggctcgg tgaccgcgaa gcaggcgcgc
     1081 tccttgcccg caatcagcgg cggcagcatg cggcgttttt gttcgtcatt cccaaaaacg
     1141 acgactgggt taaggccgaa gatattcata tgcacggccg aggcgccgga gaacccggcg
     1201 cccgattcgg cgatcgcctg catcatgaca gccgcgctgg agattcccag gccggcgccg
     1261 ccgtaggcct cgggcatcgc gatgccgagc catccgtcgc gtgcgaacgc ctggtggaag
     1321 tcaaccggaa agccgccctc gcggtcgcgc gtgagccagt agtcgtcgcc gaagcgctcg
     1381 cacagcctgg cgatcgcttc gcggatgcgt tcctgctcag gactggacga gaactccatg
     1441 cctcaccaga tccgcgatct gcgcatcgtc gaagccgagc tcgaccagaa tctcgcgcga
     1501 atgctcgccg agccgcggca cgcccaagcg cagctcgccg ccgcgccggc cgtccgccca
     1561 caccggaaac agggcgaagc cgcccgcgac ggcctcgcgt gccttcaatt gagccgtatc
     1621 ggccgactgc gccggagtga ggcacagatc gaccggcacg tcgttggcgt cgaggagctc
     1681 gagccactcg gccgcagttt tctttttgaa gatcttctcg aggatcccgg aaagcgcatc
     1741 gccgttggca cgccgcttcg catccgtcga aaacaattct gcgtcgaaac ccggcacgca
     1801 acgcacgaag ctctgccaga aatgctcctc gaggatgccg agcgtcagcc gcttgccatc
     1861 ggcggtctcg aacacgtcgt tcaccgggaa gatgtgagcg cgcgggtcga cttgcgcatc
     1921 gaggctgtgg cgcatcgccg ccaggaagaa cgccgcttcg aacagcgaca ggtcgagata
     1981 ggcgcccttt cccgagcgct ctctctcgtg cagcgccgac agcaccgcga tggcggcaaa
     2041 gctgccgccg ctcatgtcgg cgatcggcag cgacgagcgc gccggcggct tcagccactg
     2101 gcccgcaaac gccaggccgc ctgcggcggc gacgtaattc acgtcgtggc cgggccgctc
     2161 gcgccacggc ccggtctgcc cgtagccgga gatggagcag tagatgatct tcgcgttgtg
     2221 ctttttcagg ttcgggtaat cgattcccag gcgcgcggcg acccccggcc ggaagccttc
     2281 gatgacgatg tccgcctgct tcaccagctt ttcaaccacc gcggcggaat ccggctgctt
     2341 gagatcgacg gcaaggctgc gcttgttgcg attttccgtg tcgaactgga ccgggacgta
     2401 cgcgcgcccg ggatcgccgc gcggcggctc gaccttgacc accgcggcgc cgaggtcggc
     2461 gaggatcgcg gaggcgaacg gccccgggac gaacatcgag aagtccacca cccggatacc
     2521 caggagcggc agccaggcgc cgctcattgg gggggctcga tccagttgca cagcgccatg
     2581 ccgccgtcga ccaccatcga ggcgccggtg acataggcgc cgaagcgctc tgacaggacc
     2641 gcgaccgcca tggcggcgac gtcggccggc gtgccgaagc gcccgagcgg gattttttcc
     2701 accatccccg cgggcgcctc gaagccgccg cccgggatcg cgccgggcgc gatcgcattg
     2761 acgcggatgc cgtgcggacc gagcccccgg gcgagctcct tcaccagcat ggtctcgccc
     2821 gccttcgccg cgctgtagtg cggcaggttg cgcggcgact ccgcgtgcag ggaagtaatg
     2881 agcagcatgc gtcctcgaat tctttctttt ttcatatgca gtcccagaac ttgagtgagg
     2941 acgaacgcgg catccagatt gaccgcgagc atctcgcgcc actgctcgaa agtcgtagcg
     3001 aggacggtct gagtctcctg ccgccgcggg ctcgcgcagt ggacgaagac cgaaaccttt
     3061 cccagcaggc ggatcgcctc ctgcgcgagg cgccgcgcac cttcggcgct ggacaggtcg
     3121 cagaccacgt ccgctttctc gatgtcggcg cgcagcacgc gcgccccctc ttcctctagc
     3181 gccgcggcga tgccgcggcc gatgccgcgg gcggcgccgg tgacgagcgc ggtgtcgcct
     3241 tccagcagct tcatcaggaa aggccggcct tggcgcgctt cgcggcaagg cgctcgcgcg
     3301 tcttcgccgt ctccaccacg aagcgcacgt gccggccgcg gccgcattcc tcgaccgggt
     3361 ccttcaccga gaaatcgaac accaccttgc gccggtccac ctcggtcacc cgcgcctcaa
     3421 tggtcacttt caaacccagc ggcgtggcgg cgaggtggtc gatcgagacg tgcgcgccga
     3481 ccgaatcctc gccggcatcg aggtgctcga ggaggaaatc gcggcaggcg tactcgacgt
     3541 cgctcaccat gcgaggggtc gcgtagacca tgccttcctt gcccatgaag ccgatgcagc
     3601 gcgcttcgtc cacgaggata gatttcgaga acgataatcc cggttttaat gtgcttttca
     3661 tcggccgcca ttgtagcggt gcatcgcgag acctcatctc gcgtttcgga tcatctcggc
     3721 acgcgcctca gtcgccgccc aggccgcggc cggtgaggtg caaaatcgca agatgcagaa
     3781 tcaggacga
//
LOCUS       DASPXW010000008         2042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_632125, whole genome shotgun sequence.
ACCESSION   DASPXW010000008 DASPXW010000000
VERSION     DASPXW010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632125"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2042
                     /locus_tag="VEL04_00165"
     CDS             <1..>2042
                     /locus_tag="VEL04_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYT46808.1"
                     /translation="EAAKLRDEYTSTEHLLLGVMAQGGASLKRIFQTHGLKPDAVLKA
                     LAELRGNQRVTDPNPEDKFQTLEKYGRDLTALARQGKIDPVIGRDEEIRRVMQVLTRR
                     TKNNPVLIGEPGVGKTAIAEGLARRIVSGDVPESLKNKRLVAMDLGAMIAGAKYRGEF
                     EDRLKAFLKEVTASEGRIILFIDELHTIVGAGKAEGAVDAGNLLKPQLARGELRCIGA
                     TTLDEYRKYIEKDPALERRFQPVLVAEPTVEATIAILRGLKERYEVHHGVRIQDAALI
                     AAATLSHRYISDRFLPDKAVDLMDEAASRLRMELDSMPTEIDQLERQILQLEIEQSAL
                     KKEKDEASRTRLKKLEKQLADLKEQSARLKTQWQNEKAAINAASLVNSQIEQAKAELE
                     KAQRTGDLNLAAQIQYGKLPDLQKKLAAAEKALHEQPAGKRLLSQEVTEEDIANVVAS
                     WTGIPVTRMLEGERQKLAKMEERLAARVVGQGEAITAVSNAVRRARSGLQDPNRPIGS
                     FIFLGPTGVGKTELARALAEFLFDDENNIVRIDMSEYMEKHSVSRLIGAPPGYVGYEE
                     GGQLSEAVRRRPYCVVLFDEIEKAHHDVFNVLLQVLDAMRNHFKPEFVNRIDEIVVFH
                     PLSQEHIAEIVGLQVEQLAGRLAERGLTIELTDAAKDYLARTGYDPDYGARPLKRVL"
BASE COUNT          431 a          655 c          666 g          290 t
ORIGIN      
        1 gaggccgcca agctccgcga cgagtacacg agcaccgagc accttctctt gggtgtgatg
       61 gcgcagggcg gggcgtcgct caagcggatc tttcaaacgc acggcctcaa acccgatgcc
      121 gtgctcaagg ccctcgccga attgcgcggc aatcagcgcg tgaccgaccc gaatccggag
      181 gacaagttcc agacgctcga gaaatacggc cgggacctta cggcgctggc ccgccagggg
      241 aagatcgatc ccgtgatcgg ccgggacgaa gagatccgcc gcgtgatgca agtgctcacg
      301 cgccggacca aaaacaatcc ggtgctcatc ggcgagccgg gcgtgggcaa gacggccatc
      361 gccgagggac tggcccggcg catcgtgagc ggcgacgtgc cggagtcgct caaaaacaag
      421 cggctcgtgg cgatggatct cggggcgatg atcgcgggcg cgaagtaccg cggggagttc
      481 gaggaccggc tcaaggcgtt tctcaaggaa gtcaccgcta gtgaaggccg gatcattctc
      541 ttcatcgacg agctgcacac catcgtcggc gccggcaaag cggaaggcgc tgtggacgcg
      601 ggcaacctgc ttaagccgca actggcgcgc ggggagttgc ggtgcattgg cgcgacgacg
      661 ctcgacgaat accgcaaata catcgagaag gatcccgcgc tggagcggcg ctttcagccg
      721 gtgctggtcg ccgagccgac cgtcgaggcg accatcgcca tcctgcgcgg gctcaaggaa
      781 cgttacgagg tccaccacgg cgtgcgcatc caggacgccg ccctgatcgc ggcggccacg
      841 ctgtcgcacc gctacatcag cgaccgcttc ctgcccgaca aggcggtgga cctgatggac
      901 gaagccgctt cgcgcttgcg catggaattg gattcgatgc cgaccgagat cgatcaactc
      961 gaacgccaaa tcctgcagct tgagatcgag cagagcgccc tcaagaaaga gaaggacgaa
     1021 gcatcgcgaa cgcgattgaa gaagctggaa aaacagcttg ccgacctcaa ggaacagtcc
     1081 gccaggctca agacccaatg gcagaatgag aaggccgcca tcaacgccgc cagcctcgtc
     1141 aacagccaga tcgagcaggc caaggcggag ttggaaaaag cccagcgcac cggcgacttg
     1201 aatctcgcgg cgcaaatcca atacggaaag ctgcccgact tgcagaagaa actcgcggct
     1261 gccgagaaag cgttgcacga gcagccggcc ggcaagcggt tgctttcgca ggaagtcacc
     1321 gaggaagaca tcgcgaacgt cgtcgcgagc tggaccggga ttccggtaac gcggatgctc
     1381 gaaggcgagc gacagaaact ggcgaagatg gaggagcgcc tcgccgcgcg cgttgtgggc
     1441 cagggcgagg ccatcacggc cgtctccaat gccgtgcgcc gcgcccgcag cggactccag
     1501 gatccgaacc gcccgatcgg ctccttcatt ttcctcggcc ccaccggcgt cggcaaaacc
     1561 gaactggccc gcgcgctggc ggagtttctc tttgacgacg agaacaacat cgtgcggatc
     1621 gacatgagcg agtacatgga gaagcacagc gtgtcgcggc ttatcggcgc gccgccgggc
     1681 tacgtcggtt acgaggaggg cggccagctc agcgaagcgg tgcgccgccg gccgtattgc
     1741 gtggtgctgt tcgacgaaat cgagaaggcg caccacgacg tgttcaacgt gctgctccag
     1801 gtgctcgacg ccatgcgcaa ccacttcaag cccgagttcg tcaaccgcat cgacgagatc
     1861 gtggtgttcc acccgctctc gcaggagcac atcgccgaga tcgtcggcct ccaggtcgag
     1921 cagctcgccg gtcgtctggc cgagcgcggc ctcaccatcg agctgaccga cgcggcgaag
     1981 gactacctcg cccgcaccgg ctacgacccc gactacgggg cccggccgct caagcgggtc
     2041 ct
//
LOCUS       DASPXW010000009         2409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1767021, whole genome shotgun sequence.
ACCESSION   DASPXW010000009 DASPXW010000000
VERSION     DASPXW010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1767021"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VEL04_00170"
     CDS             complement(<1..402)
                     /locus_tag="VEL04_00170"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HYT46809.1"
                     /translation="MAVANRERVLRAATSSFLARGYGSSVDDIARRAGVAKQTVYHHF
                     PSKDSLFREVARELAMSVLVELEGDDVRAALLRFALAYRERALGAQGIAAFRTLVPAA
                     PRFKGLAKSMYANTAGELVQRLAQYLGAAMRD"
     gene            complement(447..1745)
                     /locus_tag="VEL04_00175"
     CDS             complement(447..1745)
                     /locus_tag="VEL04_00175"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HYT46810.1"
                     /translation="MSARNVVVIGTQWGDEGKGKIVDWLTDRAQGVVRFQGGHNAGHT
                     LVIAGKKTVLHLIPSGILRGTQCYIGNGVVLSPHALLQEMDELEAAGVELDGKLCISE
                     ACPLILPYHAALDLAREAAQGEKKLGTTGRGIGPAYEDKVARRALRLQDLFKPVRFAE
                     KLQGLLEFHNFVLQHFYKQPGIDFQKTLDETLALAQRIVPLVADVPRALYEANRNGKN
                     LLFEGAQGSLLDIDHGTYPFVTSSSCVAGAAAAGAGIGPHQLHYVLGITKAYTTRVGG
                     GPFPTELSDGVGEMLRQRGQEFGATTGRPRRTGWFDAAALKRSIQLNGCSGLCITKLD
                     VLDGAEAVKICVGYEVDGKLSDILPVGAEELSLCVPVYYELPGWMESTLGVKDYKGLP
                     KAARKYLRRIEELCALPIDMISTGAERDETIVMRHPFDPA"
     gene            complement(1819..>2409)
                     /locus_tag="VEL04_00180"
     CDS             complement(1819..>2409)
                     /locus_tag="VEL04_00180"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HYT46811.1"
                     /translation="LEADLEVQRLLCEALAAARVRGARLDIGHVAVFRSIARAAEVAP
                     EREAELFEALQKKDLPQLKALTKKLHAKTREALLLLPELYGGAEILETAARKLPKIDA
                     LQKALATLRALAKACTIPASFDLAELRGYRYHSGVVFDAYCDGVTGSVARGGRYDEVG
                     KAFGRARPATGFSIDLRSLASVAPLTRGARAKRAGG"
BASE COUNT          360 a          824 c          826 g          399 t
ORIGIN      
        1 atcgcgcatc gccgcgccga ggtactgcgc caggcgctgc accagctcgc cggcagtgtt
       61 ggcgtacatc gacttggcga ggcccttgaa gcgcggcgcc gccggcacca gcgtgcggaa
      121 ggcagcgatg ccctgcgcgc ccagcgcgcg ctcgcggtag gcgagcgcga agcgcagcag
      181 cgcggcgcgc acgtcgtcgc cctcgagctc gaccagcacg ctcatggcga gctcgcgcgc
      241 gacctcgcgg aacaggctgt ccttgctcgg gaagtggtgg tagacggtct gcttggccac
      301 gccggcgcgg cgcgcgatgt cgtcgacgct cgagccgtag ccacgcgcga gaaaagagct
      361 ggtggcggcg cgcagcacgc gctcgcgatt ggcgacggcc atgcaaggga ccctgttaga
      421 ctagaccgta cagtctagca gcaatgctag gccgggtcga acgggtggcg catgacgatg
      481 gtctcgtcgc gctcggcgcc ggtggagatc atgtcgatcg gcagcgcgca cagctcctcg
      541 atgcgccgca ggtacttgcg cgccgccttg ggcaggccct tgtagtcctt caccccgagc
      601 gtgctttcca tccagccggg cagctcgtag tacaccggca cgcagaggct gagctcctcg
      661 gcgccgaccg gcaggatgtc ggagagcttg ccgtcgacct cgtagccgac gcagatcttc
      721 accgcctcgg cgccgtcgag cacatcgagc ttggtgatgc acaggccgga gcagccgttc
      781 agctggatcg agcgcttgag cgccgcggcg tcgaaccagc cggtgcgccg cggccgcccg
      841 gtggtggcgc cgaattcctg gccgcgctgg cgcagcatct cgccgacgcc gtcggacagc
      901 tcggtcggga acggcccgcc gccgacgcgc gtcgtatagg ctttggtgat gcccagcaca
      961 tagtggagct gatgcggccc gatgcccgct ccggccgctg cggcgccggc gacgcagctg
     1021 gacgaggtga cgaagggata ggtgccgtgg tcgatgtcga gcagcgagcc ctgcgcgcct
     1081 tcgaacagca ggttttttcc gtttcgattg gcttcataaa gcgcccgcgg cacatccgcg
     1141 accagcggca cgatgcgctg cgccagagcc agcgtctcgt cgagcgtctt ctgaaagtcg
     1201 atcccgggct gcttgtagaa atgctgcagg acgaaattgt ggaattcgag cagtccctgc
     1261 agcttctccg cgaagcgcac cggcttgaac agatcctgca gcctgagcgc gcgccgcgcc
     1321 accttgtcct cgtaggccgg cccgatgccg cgcccggtgg tgccgagctt cttctcgccc
     1381 tgcgccgcct cgcgcgccag gtcgagcgcg gcgtggtagg gcaggatcag cgggcaggcc
     1441 tccgagatgc agagcttgcc gtcgagctcg acgccggcgg cctcgagctc gtccatttcc
     1501 tgcagcagcg cgtgcggcga gagcaccacg ccgttgccga tgtagcactg cgtgccgcgc
     1561 aggatgcccg aaggaatcag gtgcagcacg gttttcttgc ctgcgatcac cagcgtgtgg
     1621 ccggcgttgt gcccgccctg gaagcgcacc acgccctgcg cgcggtcggt cagccagtcg
     1681 acgatcttgc ccttgccctc gtcgccccac tgcgtgccga tgacgacgac gtttctcgcg
     1741 ctcatgattt tgtactgccc ccctgacaag gggggtggcc gaaggccggg gggtttcgtt
     1801 tttcggcgtg caaaatcgtc aaccccccgc tcgcttcgct cgcgcccccc ttgtcagggg
     1861 ggcgacagaa gcgagacttc gcaaatcaat cgagaatccc gtcgccgggc gggcacggcc
     1921 gaaagccttg ccgacctcgt cgtagcgacc gccgcgcgcg accgatcccg tcacgccgtc
     1981 gcaataggcg tcgaacacca cgcccgaatg gtagcggtag ccgcgcagct cggcgaggtc
     2041 gaagctcgcc gggatggtgc atgccttggc gagcgcgcgc agggtcgcca gcgccttttg
     2101 cagtgcatct attttcggca gcttccttgc ggcggtctcc aggatctcgg cgccgccgta
     2161 gagctcgggc aggagaagca gggcctcgcg cgtttttgca tgcagttttt tcgtcagcgc
     2221 cttcagctgc ggaaggtctt ttttctgcag cgcttcgaac agctcggcct cgcgctcggg
     2281 cgcgacctcg gcggcgcgcg cgatcgagcg gaacaccgcg acgtggccga tgtcgaggcg
     2341 cgcgccgcgc acgcgtgccg ccgcgagcgc ctcgcacagc agccgctgca cctcgaggtc
     2401 ggcctcgag
//
LOCUS       DASPXW010000010         4826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_885835, whole genome shotgun sequence.
ACCESSION   DASPXW010000010 DASPXW010000000
VERSION     DASPXW010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885835"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1356
                     /locus_tag="VEL04_00185"
     CDS             <1..1356
                     /locus_tag="VEL04_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT46812.1"
                     /translation="ELRGARMSMIFQEPMSALNPVMSCAGQIDEVLRFHTSLGKARRK
                     EKSIDLLREVALPDPERILASYPHQLSGGQRQRIGIAMALALEPALLIADEPTTALDV
                     TTQAQILRLLIELQRRHGMAVLFVTHDFGVVAEIAHRVAVLREGRLVELGTKAEVLTR
                     PQHDYTRMLIGAVPTLKPHGRAADQNSILVLETRNLSKTYVDRGWFAKRRQVRAASDV
                     SIGVRKGQTLGIVGESGSGKSTLARCIVRLIEPTSGEIRLGGTDLARMRPRELRPLRR
                     RVQIVFQDPYRSLNPRRRVGEAIIEGPLNYGVPREAALERARHLMELVRMDPAALGRY
                     PHQFSGGQRQRICIARALAVEPELLVADEAVSALDVSVQAQVLALLEEIRTRLDLAML
                     FITHDLRVAAQVCDQVAVMQAGRIVEYGPAQEVFLNPRHEYTRALFAAAPGRNFAFGA
                     A"
     gene            1367..2128
                     /locus_tag="VEL04_00190"
     CDS             1367..2128
                     /locus_tag="VEL04_00190"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HYT46813.1"
                     /translation="MDRLDAAAIALPGVEVRVVERCASTNSVLLNNADTSPVLLAAEE
                     QTAGRGRRGRRWHSAPGAGVTFSLARSLNRPAREAAGLPLVAGVAVARALRGLGATRA
                     ALKWPNDLVAAGAKLGGILVETRSQGHATRAVIGIGLNCRHDAGLELRLRRKLGALDQ
                     FIGRIDRNQIIARVARSLLDALDEFEAKGLDSARRDWEAMDAHAGQRLRVRLADGRVL
                     TGVASGLDADGGLRLATRAGLRLVRSGRVVSTRPA"
     gene            2125..2907
                     /locus_tag="VEL04_00195"
     CDS             2125..2907
                     /locus_tag="VEL04_00195"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HYT46814.1"
                     /translation="MILAIDAGNSRVKWGWLEAADEGRDRHDGELRWASIATVSLIEF
                     AASSDHINPFSITHADPERIVIANVAGEGAHQLLVNWTSIFDAEPLWLRGEAERCGVQ
                     NGYERPELLGPDRWAALIAARALHNGPSLVVNAGTATTVDMLSADGGFLGGAILPGVE
                     LMRFVLHEHTGRLPIQEGAYRDTPRNTVDAIETGCRHAQAGAVERLYRVFRDLAGTPL
                     CIVAGGAGRALVDQLSMPRRYVENLVLDGLARIALSERAVTR"
     gene            2904..3557
                     /locus_tag="VEL04_00200"
     CDS             2904..3557
                     /locus_tag="VEL04_00200"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HYT46815.1"
                     /translation="MRTAFLLLVLANLAFFAWSRYVAPPDAADPLPLSRQIEPGKLKI
                     IAPADLPPPAAQKAAAAQPVTASKCMEWGSFTLADAPRAEKALEPLALGARLAPRRTE
                     ELAAWWVFIPPQTNRQAAQKKAAELKGLSVDDYFIVQEDGEHRWALSLGVFRTEEAAQ
                     ARLAALRTQGVRSAQVGRRETVVPKVWLQVKAVDPALEARLKDIARQIEGSELKSCP"
     gene            complement(3567..3950)
                     /locus_tag="VEL04_00205"
     CDS             complement(3567..3950)
                     /locus_tag="VEL04_00205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYT46816.1"
                     /translation="MPASILVVDDDASIRQLLQLHLSAAGYEVHVAADAIVAGYLVLR
                     SPPDLIISDVNMPHMDGFEFIAALKADKTLPEIPVIFLTSMEEGSDRGKSLGVVGYIT
                     KPVRADRLLQLVAKHVPGGGAHPIG"
     gene            complement(3962..4037)
                     /locus_tag="VEL04_00210"
     tRNA            complement(3962..4037)
                     /locus_tag="VEL04_00210"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4002..4004),aa:Thr,seq:cgt)
     gene            4215..4685
                     /locus_tag="VEL04_00215"
     CDS             4215..4685
                     /locus_tag="VEL04_00215"
                     /inference="COORDINATES: protein motif:HMM:NF028042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HYT46817.1"
                     /translation="MRSRGPDHGCRGFTLIELLIVVAVIGILSVIAYPSYQRYLQRAH
                     RADAEQFMARIDTRQKQVMLEQRAYAQAIGALNLPGWTCTSTTTIPGTCTNAYYSITF
                     NPAVDNTTAPPSYSICAVPTGGQVSDGYLMLDSTGSKRRATAGSCAAPGADLGW"
     gene            4694..>4826
                     /locus_tag="VEL04_00220"
     CDS             4694..>4826
                     /locus_tag="VEL04_00220"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HYT46818.1"
                     /translation="MNARRARGFTILELMMVVAVVAVLAAIALPNLSDVIVRTRLKTA
                     "
BASE COUNT          757 a         1669 c         1702 g          698 t
ORIGIN      
        1 gagcttcgag gcgcgcgcat gtcgatgatc ttccaggagc cgatgagcgc gctcaacccg
       61 gtaatgagct gcgccggcca gatcgacgag gtgctgcgct ttcacacctc tctaggcaag
      121 gcccggcgga aagaaaaatc gatcgatctg ctgcgcgagg tcgcgctgcc ggaccccgag
      181 cgcatcctcg cctcgtatcc gcaccagctt tcgggcggcc agcgccagcg catcggcatc
      241 gcgatggcgc tcgcgctcga gccggcgctg ctcatcgcgg acgagccgac caccgcgctc
      301 gacgtgacca cgcaggcgca gatcctgaga ctgctgatcg agctgcaaag gcgccacggc
      361 atggcggtgc tgttcgtcac gcacgatttc ggcgtcgtcg ccgagatcgc gcaccgcgtc
      421 gcggtgttgc gcgaaggcag gctggtcgag ctgggcacca aggccgaggt gctgacgcgg
      481 ccgcagcacg actacacgcg catgctgatc ggcgcggtgc cgacgctgaa gccgcacgga
      541 cgcgctgcag accagaattc gatcctggtc ctggaaacga gaaatctctc gaaaacctat
      601 gtcgaccgcg gctggttcgc caagcgcagg caagtgcgcg cggcgagcga cgtcagcatc
      661 ggggtgcgca aaggccagac gctcggcatc gtcggcgagt cgggatcggg caagtcgacg
      721 ctcgcgcgct gcatcgtgcg gctgatcgag cccaccagcg gcgagatccg cctcggcggc
      781 acggacctcg cgcgcatgcg gccgcgcgag ctgcgcccgc tgcggcgccg cgtgcagatc
      841 gtgttccagg acccctaccg ctcgctcaac ccgcggcgcc gcgtgggcga ggcgatcatc
      901 gaagggccgc tcaactacgg cgtgccgcgc gaggcggcgc tcgagcgcgc ccgccatctt
      961 atggagctgg tgcgtatgga ccccgccgcg ctcgggcgct acccgcacca gttctccggc
     1021 ggacagcggc agcgcatctg catcgcgcgc gcgctcgccg tggagcccga gctgctggtc
     1081 gccgacgagg cggtctcggc gctcgacgtg tcggtgcagg ctcaggtgct ggcgctgctg
     1141 gaggagatcc gcacccggct cgacctcgcg atgctgttca tcacccacga cctgcgggtc
     1201 gcggcgcagg tctgcgacca ggtggcggtg atgcaggccg ggcgcatcgt cgagtacggc
     1261 ccggcgcagg aggtcttcct caaccccagg cacgagtaca cgcgggccct gttcgccgcc
     1321 gccccggggc gcaacttcgc tttcggcgcc gcgtaaacta gcggccatgg accgtctcga
     1381 cgcagcggcg atcgcgctcc ccggagtaga ggtgcgcgtg gtcgagcgct gcgcgtcgac
     1441 caattccgtc ctgttgaaca atgccgatac cagcccggtg ctgctcgccg ccgaggagca
     1501 gaccgcgggc cgcggccggc gcggccggcg ctggcacagc gcgccgggcg caggcgtcac
     1561 cttctccctc gcgcgcagcc tgaaccggcc ggcgcgcgag gccgcgggcc tgccgctcgt
     1621 cgcaggcgtc gcggtggcgc gtgccctgcg cgggcttggc gcgacccggg cggcgctcaa
     1681 gtggccgaac gacctggtcg ccgcgggcgc gaagctcggc ggcatcctgg tcgagacgcg
     1741 cagccagggg cacgcgacgc gcgcggtgat cggcatcggc ctcaactgcc ggcatgacgc
     1801 cggtctcgag cttcgcctgc gccgcaagct gggggcgctg gatcaattca tcgggagaat
     1861 agaccgcaac cagatcatcg cgcgcgtcgc gcgatcgctc ctcgatgcgc tcgacgaatt
     1921 cgaggccaaa ggactcgaca gcgcgcggcg cgactgggag gcgatggacg cgcacgccgg
     1981 gcagcgcctg cgggtgcggc tcgccgacgg ccgcgtgctc accggggtgg cgagcgggct
     2041 cgacgccgat ggcggcctgc gcttggcgac gcgcgcggga ttgcgcttgg tgcgcagcgg
     2101 ccgcgtcgta tctacgaggc cggcatgatc ctcgccatcg acgccggcaa ctcgcgcgtg
     2161 aaatggggat ggctggaagc cgccgatgaa ggcagggacc ggcacgacgg cgagctgcgc
     2221 tgggccagca tcgcgacggt gtcgctgatc gagttcgccg cctcgagcga ccacatcaac
     2281 ccgttctcga tcacgcacgc cgatcccgag cgcatcgtga tcgcgaacgt ggccggagaa
     2341 ggcgcgcatc aactgctcgt caactggacc agcatcttcg acgccgagcc gctgtggctg
     2401 cgcggcgagg ccgagcgctg cggggtgcag aacggctacg agcggcccga gctgctcggg
     2461 ccggaccgct gggcggcgct gatcgccgcg cgggcgctgc acaacgggcc gtcgttggtg
     2521 gtcaacgcgg gcaccgccac cacggtcgac atgctctcgg ccgacggcgg cttcctcggc
     2581 ggcgcgatcc tgcccggcgt ggagctgatg cgcttcgtgc tgcacgagca caccgggcgg
     2641 ttgccgatcc aggaaggcgc gtaccgcgac acgccgcgca ataccgtcga tgccatcgaa
     2701 accggctgcc gccacgccca agcaggagcg gtcgagcggc tgtaccgcgt gttccgcgac
     2761 ctcgcgggca cgccgctgtg catcgtcgcc gggggcgccg ggcgcgcgct cgtcgaccag
     2821 ctctcgatgc cgcgccgcta cgtggagaac ctggtgctcg acggcctggc gcgcatcgcg
     2881 ctgagcgagc gcgccgtcac gcgatgagga cggcttttct cctgctggtg ctcgccaacc
     2941 tggcgttttt cgcctggtcg cgctacgtcg cgccgcccga cgctgccgat ccgctgccgc
     3001 tgtcgcgcca gatcgagccc gggaagctga agatcatcgc cccggccgac ctgccgccgc
     3061 ccgcagcgca gaaagccgcc gcagcgcagc cggtaacggc gtccaagtgc atggaatggg
     3121 gcagcttcac gctcgccgac gcgccgcgcg ccgagaaggc gctcgagccg ctcgcgctcg
     3181 gcgcgcgcct cgcgccgcgc cgcaccgagg agctcgcggc ctggtgggtg ttcattccgc
     3241 cgcaaaccaa ccgccaggcg gcgcagaaga aggccgccga gctgaaaggg ctcagcgtcg
     3301 acgactactt catcgtgcag gaagacggcg agcaccgctg ggcgctgtcg ctcggcgtgt
     3361 tccgcaccga ggaagcggcg caggcgaggt tggcggcact gcgcacccag ggggtgcgca
     3421 gtgcccaggt cgggcggcgc gaaaccgtgg tgccgaaggt ctggctgcag gtgaaggccg
     3481 tcgacccggc gctcgaggcc aggctgaagg acatcgcccg ccagatcgag ggcagcgagc
     3541 tgaagagctg cccttaacct gcctttttag cctatgggat gcgcgccgcc gcccggcacg
     3601 tgcttcgcga ccagctgcag cagccggtcg gcgcgcaccg gcttggtgat gtagcccacc
     3661 acaccgagcg acttgccgcg atcgctgccc tcctccatcg acgtcaggaa gatcaccggg
     3721 atctcgggca gcgtcttgtc ggccttgagc gcggcgatga actcgaagcc gtccatgtgc
     3781 ggcatgttga cgtcggagat gatgaggtcg ggcgggctgc gtagcaccag gtagccggca
     3841 acgatcgcgt ccgcggcgac atgcacctcg tagcccgccg cggaaaggtg gagctgcagc
     3901 agctggcgga tgcttgcgtc gtcatcgact acgaggatgc tggcgggcat tgaggcattc
     3961 ttggtgctgg tggcccgact cgaacgggcg acctactgat tacgaatcag ttgctctacc
     4021 agctgagcta caccagcgat acggggagcg gcggattata cgccaggcgg tgggcggccc
     4081 cgcgggttgt gaactgcatc acgcatactg cgccgcgtcg cgccgttcgg tccgcgataa
     4141 gggccgccgg tcgccgcttt ttgcggcgcc gggcggcact ccgtagcatc ccaaccacaa
     4201 gaaagggaga cgtcatgcgc agcaggggac ccgatcacgg ctgcaggggt ttcactctca
     4261 tcgaattgct gatcgtggtg gccgtgatcg gcatcctttc ggtcatcgcc tatccgtcct
     4321 atcagcgcta cctccagcgc gcccaccgcg cggacgccga gcaattcatg gcgcggatag
     4381 acacgcggca gaagcaggtc atgctcgagc agcgcgccta tgcgcaggcc atcggcgccc
     4441 tgaacctgcc cggctggacc tgcaccagca ccacgaccat tccgggaacc tgcaccaacg
     4501 cctattactc gatcaccttc aaccctgcgg tcgacaatac cacggcaccg ccgagctatt
     4561 cgatctgcgc ggtgccgaca ggcggccagg tgagcgacgg ctacctgatg ctcgacagca
     4621 ccggctccaa gcgcagggcg acggccggca gctgcgccgc gccgggcgcg gacctgggct
     4681 ggtgaaaacc gtcatgaacg ccaggcgcgc gcgcggcttc acgatcctcg agctgatgat
     4741 ggtggtggcg gtagtcgcgg tgctcgccgc aatcgcattg ccgaacctga gcgacgtgat
     4801 agtgcgcacg cgcctgaaga ccgcgg
//
LOCUS       DASPXW010000011         3884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1012306, whole genome shotgun sequence.
ACCESSION   DASPXW010000011 DASPXW010000000
VERSION     DASPXW010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012306"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..126)
                     /locus_tag="VEL04_00225"
     CDS             complement(<1..126)
                     /locus_tag="VEL04_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HYT46819.1"
                     /translation="MPIYEYRCSSCGFQKEYLQKVTDPVKTVCPECGKKTFSKMLT"
     gene            complement(249..1955)
                     /gene="soxB"
                     /locus_tag="VEL04_00230"
     CDS             complement(249..1955)
                     /gene="soxB"
                     /locus_tag="VEL04_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfohydrolase SoxB"
                     /protein_id="HYT46820.1"
                     /translation="MSLSRREFLHTLGAAAAAGFALDARTASQASSFYDVPRFGNVSL
                     LHFTDCHAQLLPVHFREPNVNLGVGGARNRPPHLVGEALLKHFHLRPGSREAHAFTHL
                     DFARAAKTYGKLGGFAHLATLVKRLRAGRRASLLLDGGDTWQGSATSLWTRGQDMVEA
                     QKLLGVDIMTGHWEFTYGAARVKEVVERDFAGRIEFLAQNVETSDFGDPVFKPYAVRE
                     LAGVPVGIIGQAFPYTPIAHPRRFVAEWKFGIEEERLQRLADEIRGRGAQVVALLSHN
                     GMDVDLKLASRVAGIDVILGGHTHDGVPEPVVVSNAKGRTLVTSAGSNGKFLAVLDLD
                     IRGGRISGHRYRLLPVFANLLPADPEMARLIEAQRAPYAGKLSEKLAVTEAPLYRRGN
                     FNGSFDQLILDALMEVKGAEIAFSPGFRWGTTLLAGEAITLESVMNQTAITYPDVTLT
                     EMSGARLKAILEDVCDNLFNPDPYYQQGGDMVRVGGMTYSCAPGAKMGARIGDMRLGD
                     RPIDPGKTYKVAGWAPVAEGVRGEPIWDVLADYLRARKFVKPRRLSLPRLVGVKGNLG
                     IA"
     gene            complement(2083..2700)
                     /gene="soxX"
                     /locus_tag="VEL04_00235"
     CDS             complement(2083..2700)
                     /gene="soxX"
                     /locus_tag="VEL04_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HYT46821.1"
                     /translation="MRLDVVAAAALCAAMLFGCATRASDAEAERAALAMMKTSFRAQG
                     QAGVDRLDQDEVQALCSRHPNGLPGDLAEKLEKAQLATIRYPASGKLMGDWREGERIA
                     QSGVGKQFNDDPSRPSGGNCYACHRLSSQELSFGTIGPSLYRFGKERGTGEAVRRYAY
                     GKIYNPEAFSACSNMPRFGHNGILTEQQITHLVALLLDPDSPVNK"
     gene            complement(2714..3520)
                     /gene="soxA"
                     /locus_tag="VEL04_00240"
     CDS             complement(2714..3520)
                     /gene="soxA"
                     /locus_tag="VEL04_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HYT46822.1"
                     /translation="MAKAIRIGFVALAIALPCFARAQQDTQKEIERYRELLQDGNPSE
                     LFEARGAEHWKTKRGPKNASLERCDLGRGAGVVKGAYVQLPRYFADTDKVQDLESRLV
                     SCMVALQGLSSEEAKRRPYSGPGQPSVMADLVAWIASESRGMRIAPPQSHPKERLAYQ
                     AGEKIFYYRAGPHDFACATCHGEDNKRIRLQDLPNLTSAGDAQRAFGTWPAYRVSQGE
                     VRSMQWRLYDCFRQQRFPELEFTSDAAIALISFLAKQGEGGTFEAPAIKR"
     gene            complement(3513..3830)
                     /gene="soxZ"
                     /locus_tag="VEL04_00245"
     CDS             complement(3513..3830)
                     /gene="soxZ"
                     /locus_tag="VEL04_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HYT46823.1"
                     /translation="MADAMRIRANIVGDKVEVRVLMSHEMETGQRRDARGDIVPAHFI
                     QNVTVTHAGKTVLSAQWGPAISKNPFVHFRFKGGKPGEKLTISWVDNQGDKRTDEATI
                     SGG"
BASE COUNT          610 a         1312 c         1261 g          701 t
ORIGIN      
        1 cgtcagcatc ttggaaaacg tcttctttcc gcattcggga cagaccgtct tcaccgggtc
       61 ggtgactttc tgcaggtatt ccttctggaa cccgcaggat gagcagcgat attcgtagat
      121 cggcatgaag cctccgcaag ctgcggatta tacctccaag tgatctgttc cccctgcgga
      181 gatggggaac ggcgcgacga tttcaagttc cgggacgagc ccgctgcgcg gtcgcggata
      241 atcccgattc aagcgatccc gaggttccct ttgacgccga ccaggcgcgg caggctgagc
      301 ctgcgcggct tgacgaattt ccttgcgcgc aagtaatcgg ccagcacgtc ccatatgggt
      361 tccccgcgga cgccctcggc gaccggagcc cagccggcca ccttgtaggt ctttccgggg
      421 tcgatcggcc ggtcgcccag gcgcatgtcg ccgatgcgcg cgcccatctt cgctcccggg
      481 gcgcagctat aggtcattcc gcccacccgc accatgtccc cgccctgctg gtaataggga
      541 tcgggattga acaggttgtc gcagacgtcc tcgagaatcg ccttgagcct cgcgccgctc
      601 atctcggtga gcgtgacgtc cggataggtg atcgcggtct ggttcatcac gctctccagc
      661 gtgatcgctt cgcccgcgag cagcgtcgtg ccccagcgaa agccgggcga gaacgcgatc
      721 tcggcgcctt tcacttccat cagcgcatcg agtatcagct gatcgaacga gccgttgaag
      781 ttgccgcggc ggtagagcgg cgcttcggtc accgcgagtt tttccgaaag cttgcccgcg
      841 tagggagcgc gctgtgcctc gatcagccgc gccatctcgg gatcggcggg aagcaggttc
      901 gcgaacaccg ggagcaagcg gtagcgatgc cccgagatcc gcccgccccg gatatcgaga
      961 tcgagcaccg cgaggaattt tccgttcgat cccgcgctgg tgaccagcgt cctgcctttc
     1021 gcattggaca ccaccaccgg ctccggcacg ccgtcgtggg tatggccgcc gagaatgacg
     1081 tcgatcccgg cgacgcgcga agcgagcttg aggtcgacgt ccatgccgtt gtgcgagagc
     1141 agcgccacga cctgcgctcc cctgccgcgg atttcgtctg cgagcctctg caggcgctcc
     1201 tcctcgatgc cgaatttcca ctcggcgacg aaccggcgcg gatgggcgat cggcgtgtag
     1261 ggaaacgcct gaccgatgat gccgaccggc acgccggcga gctcgcgaac ggcgtagggc
     1321 ttgaacaccg ggtcgccgaa gtcgctggtc tcgacgttct gcgcgaggaa ttcgatcctg
     1381 ccggcgaaat cgcgctcgac cacctccttc acgcgggccg cgccgtaggt gaactcccag
     1441 tgcccggtca tgatgtccac gcccagcagc ttctgcgcct cgaccatgtc ctgccctcgc
     1501 gtccacagcg aggtcgccga tccctgccag gtgtcgccgc cgtcgaggag gaggctcgcg
     1561 cggcgtccgg cgcgcagcct cttcacgagc gtcgcgaggt gcgcgaagcc gcccagcttt
     1621 ccgtacgtct ttgccgcccg cgcgaagtcg agatgggtga aggcgtgcgc ttcgcggctt
     1681 cccggccgga ggtggaaatg cttcagcaat gcctcgccga ccagatgcgg cgggcggttc
     1741 ctcgccccgc cgacgccgag attgacgttc ggctcgcgga aatggaccgg aagaagctgc
     1801 gcatgacaat cggtgaaatg cagcagcgag acgttgccga agcgcggcac gtcgtagaac
     1861 gacgaagctt gcgacgcggt gcgcgcatcc agcgcgaacc cggccgcggc ggcggctccg
     1921 agggtatgca ggaattcgcg gcgggagaga gacactagat gacgtcgtct ccgcggaagc
     1981 ggggacccag tggcgttgaa agccgctgga ttcccgtccc cgataacacc attcggggac
     2041 aggcccgcgc gggggtgacg aagcgacgct cgcgtcgctc cgtcacttgt ttaccggcga
     2101 atccggatcg agcagcagcg ccacgaggtg cgtgatctgc tgctcggtga ggatcccgtt
     2161 gtgcccgaag cgcggcatgt tggagcaggc ggagaaggct tccgggttgt agatcttgcc
     2221 gtaggcgtac cgacggaccg cctcgcctgt gccgcgctcc ttgccgaatc ggtagaggct
     2281 cggtccgatg gtgccgaacg acagctcctg ggacgagagc cggtggcagg cgtagcagtt
     2341 gcctcccgag ggccggctgg gatcgtcgtt gaactgcttg cccacgccgc tttgcgcgat
     2401 gcgctcgcct tcgcgccagt cccccatcag cttgcccgaa gcgggatagc ggatcgtggc
     2461 gagctgcgcc ttctcgagct tttccgcgag gtccccgggc agcccgttcg gatgcctgct
     2521 gcacagcgcc tgcacctcgt cctggtcgag gcggtcgacg cccgcctgcc cctgcgccct
     2581 gaacgaggtc ttcatcatgg cgagcgccgc gcgctcggcc tccgcgtccg acgcgcgcgt
     2641 ggcgcagccg aaaagcatcg ccgcgcaaag cgcggcggct gcgacgacat cgagtctcat
     2701 ggctgttttc ccttcagcgc ttgatcgccg gtgcctcgaa tgtcccgccc tcgccctgtt
     2761 tcgcgagaaa gctgatgagc gcgatcgcgg cgtcggaggt gaattcgagc tccggaaagc
     2821 gctgctggcg gaagcagtcg tacaggcgcc actgcatcga gcgcacctcg ccctgcgaaa
     2881 cgcgatacgc cggccaggtg ccgaaagcgc gctgcgcgtc cccggcgctg gtcagattcg
     2941 gcaggtcctg cagccggatg cgcttgttgt cctcgccgtg gcaggtcgcg caggcgaagt
     3001 cgtgcggccc ggcgcggtag tagaagatct tctcgcccgc ctggtacgcg agccgctcct
     3061 tgggatgcga ctgcggcggc gcaatcctca tgccgcgcga ttcggaggcg atccaggcga
     3121 ccaggtcggc catcaccgaa ggctggcccg ggccggaata gggacggcgc ttcgcctctt
     3181 cggaactaag cccctgcagc gcgaccatgc agctcaccag ccgcgattcg aggtcctgga
     3241 ccttgtcggt gtcggcgaag tagcgcggca gctgcacgta cgcgcccttc accacgcccg
     3301 cgccccgccc caggtcgcac cgctcgagcg aggcgttctt cgggccgcgc ttcgtcttcc
     3361 agtgctcggc gccgcgcgct tcgaacagct ccgacggatt gccgtcctga aggagttcgc
     3421 ggtagcgctc gatctccttc tgggtgtcct gctgagcccg cgcgaagcac ggcagcgcga
     3481 tcgccagcgc cacaaacccg atccgaatcg ccttagccac cggaaatcgt cgcttcgtcg
     3541 gtgcgtttgt cgccctggtt gtcgacccag ctgatcgtca gcttctcgcc gggcttgccg
     3601 cccttgaagc ggaaatgcac gaacggattt ttcgatatcg cgggccccca ttgcgccgag
     3661 agcaccgtct ttccggcgtg ggtgacggtc acgttctgga tgaagtgcgc tgggacgata
     3721 tcgccccgcg cgtccctgcg ctggccggtc tccatctcgt ggctcatcag cacccgcact
     3781 tcgaccttgt cgcctacgat gttggcacgg atgcgcatcg cgtccgccat tttacccccc
     3841 gcacccgccg atggtgactt tgatctcctt cacggcatgg aagt
//
LOCUS       DASPXW010000012         5877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_886356, whole genome shotgun sequence.
ACCESSION   DASPXW010000012 DASPXW010000000
VERSION     DASPXW010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5877
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886356"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="VEL04_00250"
     CDS             <1..653
                     /locus_tag="VEL04_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HYT46824.1"
                     /translation="LELFRDHRVPLTVFGVAMALERNPGVVEAFLEAGHEIASHGWRW
                     INYQYVPLEAERSHMQRAIEIHKKLTGERPLGWYTGRTSPNTRRLVVEDGGFLYDADD
                     YNDELPWYDTRYGKPQLVVPYTLDANDMRFATAQGFNSGQQFFDYLKDSFDVLYEEGR
                     RVPRMMSVGLHCRLAGRPGRVASLENFLKYVKTKSKVWFARRIDIARHWLKAHPHG"
     gene            646..1884
                     /locus_tag="VEL04_00255"
     CDS             646..1884
                     /locus_tag="VEL04_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HYT46825.1"
                     /translation="MAERIDDLNPAPRLLMGPGPINADPRVLRAMSAQLLGQFDPQFR
                     AYMRETSALYRGVFQTQNRWTLLIDGTARSAIEAALASLIEPGDRVLVPVFGRFGHLK
                     VEIAKRCGAEVHAVETDWGTVFPTEILEKEIKRHKPKIVAVSHGDTSTTMAQPLAELG
                     NLCRKNDLLLYVDATATLGGMDLPTDAWQLDVVSAGLQKCLGGPSGSAPLTLNERAEK
                     LISRRRHTEQGLKDKNQEEGEGSVIRSNYFDLAMIMDYWSDKALNHHTEATSMLYAAR
                     ECARILLQEGLAKAFARHALASRALCRGIEAMGLKLFGDPKHKMPNVTGVHIPQGVDG
                     EKVRRALLADFNIEIGTSFGPLHGRVWRIGTMGYNAREDAVLVTLGALETVLSGEKFK
                     LSRGMAVDAALAVYRDGVRG"
     gene            1868..2641
                     /locus_tag="VEL04_00260"
     CDS             1868..2641
                     /locus_tag="VEL04_00260"
                     /inference="COORDINATES: protein motif:HMM:NF022834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HYT46826.1"
                     /translation="MAFAASVAGRFARDALALGEGEVCALFRRSFYLRFPGGKYACVG
                     DPSLGRGPLNALVAPFSAPALGERISVSVADASIWRPPALPQGSPDLTALKKAAQARV
                     PAEGFGCLVAGTHNALSVHAQPALEAIDRWLVGNALGAEAAQLIGLGPGLTPSGDDYL
                     GGVMVALHALGRGTQSAGLWRWLQPRLAERTSAISAAHLTAAAAGEAHEALHACLLEL
                     FKSASGWEATLPRLGAVGHGSGWDGLAGALAVLERSSLG"
     gene            complement(2633..4018)
                     /gene="fdrA"
                     /locus_tag="VEL04_00265"
     CDS             complement(2633..4018)
                     /gene="fdrA"
                     /locus_tag="VEL04_00265"
                     /inference="COORDINATES: protein motif:HMM:NF004760.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HYT46827.1"
                     /translation="MVLNRVELGRYLDSVTLMRVSRRLGALPGVEAAAVMVGTPANRG
                     LLAEAGLLAAEGERASPSDLVLAVRAADARAAAAALKAAEGLILEQNFPVSESMPHAR
                     SLAGALELLPRANLALISVPGGFAAREARSALERGLNALVFSDDVPLEEELALKRFAN
                     EKNLLLMGPDCGTALIAGTPIAFANAVPQGDIGIVSASGTGLQEVSSLIARLGGGVSH
                     GIGVGGRDLDARIGALGTLAAFDALEQDPATSKIVLISKPPAPQVARLVLKRVKRSSK
                     PCVVCFLGSREPGLARTLTDAAELAIGKKLAPAKLSLPKRRKGLVRGLYCGGTLCAEA
                     EMIFLQKKLKHHEFIDLGDDQYRRGRPHPMIEPELRDQHIVRALADPKVAVLLFDVVL
                     GYGSHLDPAGILVHALKGSSKPSVASVTGTEGDPQGWSRQGAILRNAGVIVAPSNARA
                     AKAAAALVSSA"
     gene            complement(4008..5414)
                     /locus_tag="VEL04_00270"
     CDS             complement(4008..5414)
                     /locus_tag="VEL04_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HYT46828.1"
                     /translation="MRLLAGPLRVVNIGLAAFAHDLAAHGAAVAQVDWRPPAGGKPEL
                     LSALQKLSSSNQKIEDANREALGRILAADPVLADVARAGDLIPQLDHERLILHAGPPI
                     AWEAMCGPLRGAVCGAIVFEGWANDLGAAAALAAGGGVKFAPNHDHGAVGPMTGITTR
                     SMPLFVVENRAFGNRALCAINEGLGKVMRFGGNDAEVLARLAWLRDEFAPLLQNSLKL
                     NGGIALVPLIARGLTMGDEMHQRNVACSSLLLRILSPHLSRAAGDSSSLVKAFEFMAG
                     NDQFFLNVAMAMGKCMLDPAQGTAGSTLVTAMSRNGTEFGIRVAALGGRWFTAPVEMP
                     QGLYFPGFSAADANPDMGDSAILEAVGLGAFAMAAAPAVAGFVGAGGFQDALGYSRAM
                     SEISAARNPKWAIPALEFAGTPTGIDIRRVVETGIAPAINTGIAHRRAGVGQVGAGIA
                     RAPLVCFEKALLAISNGP"
     gene            complement(5420..>5877)
                     /locus_tag="VEL04_00275"
     CDS             complement(5420..>5877)
                     /locus_tag="VEL04_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HYT46829.1"
                     /translation="QLARFLPVLALACPEARFQPVYVGDVARAMTTALAETGSFGKRY
                     DLCGPRAYTLKELVEYVCAVTGRHRLIIGLPDRLSYLQARLLEWSPGPLMTRDNYLSM
                     QVPSVCDAGGGGCLPFGLVPQALEALAPVYLAPAGARERYPQLRWRARR"
BASE COUNT         1003 a         2006 c         1977 g          891 t
ORIGIN      
        1 tgctcgagct gttccgcgac caccgcgtgc cgctcaccgt gttcggcgtc gccatggcgc
       61 tggaacgaaa cccgggcgtg gtggaagctt tcctcgaagc cggtcacgag atcgcgagcc
      121 acggctggcg ctggatcaac taccagtacg tgccgctcga agccgagcgc agccacatgc
      181 agcgcgcgat cgagatccat aaaaagctca ccggcgagcg gccgctcggc tggtacaccg
      241 ggcgcaccag tcccaacacg cggcgcctgg tggtcgagga cggcggcttc ctctacgacg
      301 ccgacgacta caacgacgag ctgccctggt acgacacgcg ttacggcaag ccgcagctgg
      361 tggtgcccta cacgctcgac gccaacgaca tgcgcttcgc caccgcgcag ggcttcaact
      421 ccggccagca attttttgac tacctgaagg actccttcga cgtgctctac gaggaaggcc
      481 gccgcgtgcc gcgcatgatg tcggtcgggc tgcactgccg gctggcggga aggccaggac
      541 gagtcgcctc gctggagaac tttctgaaat acgtgaagac gaaaagcaaa gtctggttcg
      601 cgcggcgcat cgatatcgcg cgccactggc tgaaggcgca cccgcatggc tgaacgcatc
      661 gacgatctca atccggcgcc gcggctgctg atgggcccgg ggccgatcaa tgccgacccg
      721 cgcgtgctgc gcgcgatgag cgcccagctc ctgggccagt tcgacccgca gttccgcgcc
      781 tacatgcgcg agacctccgc gctataccgc ggcgtattcc agacgcagaa ccgctggacc
      841 ctgctcatcg acggcaccgc gcgctcggcg atcgaggcgg cgctcgcctc gctcatcgag
      901 cccggcgacc gcgtgctggt gcccgtgttc ggccgcttcg gccacctcaa ggtcgagatc
      961 gcgaagcgct gcggcgccga ggtacatgcc gtcgagacgg attggggaac ggtttttccg
     1021 acagagattc tcgagaaaga gatcaaaagg cacaaaccga aaatcgtcgc cgtgtcgcac
     1081 ggcgatacct cgaccacgat ggctcagccg ctcgccgaat tgggaaatct ctgcaggaaa
     1141 aatgacctcc tgctctacgt ggacgcaacc gcgaccctgg gcggcatgga cctgccgacc
     1201 gatgcctggc agctcgacgt ggtgagcgcc gggctgcaga aatgcctcgg cgggccttcg
     1261 ggcagcgcgc cccttacgct gaacgagcgc gccgaaaaat tgatttcgag aagaaggcac
     1321 accgagcaag gtttaaaaga taaaaatcag gaagaaggcg aaggctcggt catccgctcc
     1381 aactacttcg acctcgcgat gatcatggat tactggtcgg acaaggcgct caaccaccat
     1441 accgaggcga ccagcatgct gtacgcggcg cgcgaatgcg cccgcatcct gctgcaggag
     1501 ggtctcgcca aggcttttgc gcggcacgcg cttgcttcca gggccttgtg ccggggaatc
     1561 gaggccatgg gcctcaagct cttcggcgac ccgaagcaca agatgccgaa tgtcaccggc
     1621 gtgcacatcc cgcaaggcgt cgacggcgaa aaagtgcgcc gcgcgctgct cgcggacttc
     1681 aacatcgaga tcggcacctc gttcggcccc ctgcacggca gggtctggcg catcggcacc
     1741 atgggctaca acgcgcgcga ggatgcggtg ctcgtcaccc tcggcgcgct cgaaaccgtt
     1801 ctttcgggag agaaattcaa gctgtcgcgc ggtatggcgg tggacgccgc gctggctgtg
     1861 taccgcgatg gcgttcgcgg ctagcgtcgc ggggcgcttt gcgcgcgacg cgctggcact
     1921 cggcgagggc gaagtctgcg cgctgtttcg ccgcagcttc tatctcagat ttcccggcgg
     1981 gaagtacgcc tgcgtcggcg acccgtcgct cggccgcggg ccgctcaacg cgctcgtcgc
     2041 accgttcagc gcgccggcgc tgggcgagag aatttccgtt tcagtcgccg acgcatcgat
     2101 ctggcgtccg ccggccctgc cgcaaggttc gcctgatctg acggctctga aaaaagccgc
     2161 gcaggcgcgc gttccggcgg aaggcttcgg ctgcctcgtc gccggtaccc acaacgcgct
     2221 cagcgtccac gcccagccgg cgctcgaggc gatcgaccgc tggctggtcg gcaatgcgct
     2281 cggcgccgaa gccgcgcagc tcatcggcct gggaccgggc ctcacgccct cgggcgacga
     2341 ctacctcggc ggcgtgatgg tcgcgctgca tgcgctcggg cgcggcactc agtccgcagg
     2401 gctctggcgc tggctccagc cgcggctcgc ggagcgcacc agcgcaatca gcgcagcgca
     2461 tctcactgcg gccgcggccg gggaggccca cgaggcgctg cacgcctgcc tgctggagct
     2521 gttcaagagc gccagcggat gggaggcgac gctgccgcga ctcggcgccg tcggccacgg
     2581 ttcaggctgg gacggcctcg ccggcgccct ggccgtgctg gagaggtcga gcctaggctg
     2641 aacttacgag cgcggcggcg gcctttgcgg cgcgcgcgtt cgacggcgca acgatcacgc
     2701 cggcattgcg cagaatcgcc ccttgccgcg accagccttg cggatcgccc tcggtgccgg
     2761 tcacggaagc gacgctcggc ttgctcgatc ccttcagtgc atgaaccagg attcccgcag
     2821 ggtcgaggtg ggagccgtag ccgagcacca cgtcaaacag aagaactgcc accttggggt
     2881 cggcgagcgc gcgcacgatg tgctggtcgc gcagctcggg ctcgatcatc gggtgcggcc
     2941 ggccgcgcct gtactggtcg tcccccaggt cgatgaattc atgatgcttg agctttttct
     3001 gtagaaatat catttcggcc tcggcgcaca acgtgccacc gcagtaaagc ccgcggacca
     3061 ggccctttct tcttttcgga agactcaatt tagcgggcgc cagctttttc ccgatcgcga
     3121 gctccgccgc gtcggtcagc gtgcgcgcga ggccgggctc gcgcgagccg aggaagcaca
     3181 cgacgcaggg cttggaggag cgcttcaccc gtttcaggac gagccttgcg acctgcggcg
     3241 ccggcggctt cgagatcagg acgatcttcg aggtcgcagg atcctgctcc agggcgtcga
     3301 acgcggcgag agtgccgagc gcgccgatac gcgcgtcgag gtcgcggccg ccgacgccga
     3361 tgccgtgcga gacgccgccg ccgaggcgcg cgatgagtga gctcacctcc tgcagcccgg
     3421 tgccggacgc ggagacgatg ccgatgtcgc cctgcggcac ggcgttggca aaggcgatcg
     3481 gggtcccggc gatcagcgcc gtgccgcagt caggccccat caacaagaga tttttttcat
     3541 tggcaaagcg cttcagcgca agctcttcct cgagcggcac gtcgtcggag aagaccagcg
     3601 cgttcaggcc gcgctcgagc gcgctgcgcg cttcgcgcgc ggcgaatccc cccggcaccg
     3661 agatcagggc gagattcgcc cgcggcagca attcgagcgc accggcgagc gagcgcgcat
     3721 gcggcatcga ttcggaaacc ggaaaatttt gctcgagaat cagtccctcg gccgccttga
     3781 gcgcggccgc ggcggcacgc gcgtcagccg cgcgcacggc gaggaccaga tcgctcggcg
     3841 aggcgcgttc gccttcggcg gcgagcagcc ccgcctcggc gaggagcccg cggttggcgg
     3901 gcgtgccgac catcaccgct gccgcctcga caccgggcaa cgcgccgagc cggcgcgaaa
     3961 cgcgcatcag ggtgaccgag tcaaggtagc ggccaagctc aacccggtta aggaccattt
     4021 gatattgcca atagagcttt ttcaaaacac accagcggag ctcgcgcgat acccgcgccg
     4081 acctggccga cgcccgcccg gcggtgcgcg atgccggtat tgatcgccgg cgcgatgccg
     4141 gtctccacca cccggcggat gtcgataccg gtcggcgtgc cggcgaattc cagcgccggg
     4201 atcgcccact tcggattgcg cgccgcgctg atctcgctca tggcgcggct gtagccgagc
     4261 gcgtcctgga agccgcccgc gccgacgaag cccgcgaccg ccggcgccgc cgccatggcg
     4321 aaggcgccga ggcccaccgc ctcgaggatc gccgaatcgc ccatgtcggg gttcgcatcg
     4381 gccgcgctga accccgggaa atagaggcct tgcggcattt cgaccggcgc ggtgaaccag
     4441 cggccaccga gcgccgcgac gcggatgccg aactcggtgc cgttgcggct catcgccgtg
     4501 acgagcgtcg agcccgcggt tccctgcgcc gggtcgagca tgcacttgcc catcgccatc
     4561 gcaacattga ggaagaactg gtcgttcccc gccatgaatt cgaaagcttt caccaatgaa
     4621 gacgaatctc cggctgcacg ggaaagatga ggggaaagaa ttcgaagaag cagcgacgaa
     4681 caggcgacgt ttctctggtg catctcatcg cccatcgtca ggccgcgcgc gatgagcggg
     4741 acaagcgcaa ttccgccgtt cagtttgaga ctattttgca aaagaggggc gaattcgtct
     4801 cgcagccacg caaggcgcgc cagcacttcg gcgtcgttgc cgccgaagcg catcaccttt
     4861 cccaggcctt cgttgatcgc gcacagcgcg cggttgccga aggcgcggtt ctcgaccacg
     4921 aagagcggca tgctgcgcgt cgtgatgccg gtcatcggcc cgaccgcgcc gtgatcgtgg
     4981 ttcggcgcga acttcacccc gccgccggcg gcgagcgctg cggcggcgcc gaggtcgttc
     5041 gcccagccct cgaacacgat cgcgccgcac accgcgccgc gcagcggccc gcacatcgcc
     5101 tcccaggcga tgggcggccc ggcgtgcagg atcaagcgct cgtggtcgag ctgcgggatc
     5161 aggtcgccgg cgcgcgcgac atcggcgagc accgggtccg cggcgaggat gcggccgagc
     5221 gcctcccggt ttgcatcttc gattttctgg tttgaggaag aaagtttctg caaagccgaa
     5281 agtaattcag gcttgccgcc ggccgggggc cgccagtcca cctgcgcgac cgcagcgccg
     5341 tgcgcggcga ggtcgtgcgc gaatgccgcg agcccgatat tgaccacccg cagcgggccg
     5401 gcgagcagcc gcatcgcgtt tacctgcgcg cgcgccagcg cagctgcggg tagcgctcgc
     5461 gcgcgcccgc aggcgcgagg tagaccgggg cgagcgcttc gagcgcctgc ggcaccaggc
     5521 cgaagggcag gcagccgccg ccgcccgcat cgcagaccga gggcacttgc atcgaaaggt
     5581 agttgtcgcg ggtcatcagc ggtcccggcg accattcgag aagccgcgcc tgcaggtagg
     5641 agagccggtc cggcagcccg atgatgaggc ggtgccggcc agtcaccgcg cacacgtact
     5701 cgacaagctc tttcagcgtg taggctcgcg gcccgcacag gtcatagcgc ttgccgaagg
     5761 acccggtttc cgcgagcgcg gtggtcatcg cgcgcgccac atcgccgacg tacaccggct
     5821 ggaagcgcgc ctcggggcag gcaagcgcga gcaccggcag aaagcgcgcg agctgcg
//
LOCUS       DASPXW010000013         2462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_760441, whole genome shotgun sequence.
ACCESSION   DASPXW010000013 DASPXW010000000
VERSION     DASPXW010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760441"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(236..1192)
                     /locus_tag="VEL04_00280"
     CDS             complement(236..1192)
                     /locus_tag="VEL04_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYT46830.1"
                     /translation="MRVRFWGTRGSIPVGLTAADIRDKLAQALLAASGQRFASYGDAH
                     AFAERELPFSLGHTYGGHSSCVELETGHEEYFVCDMGSGARAFGAHVLARQGGRPATI
                     NVFMSHVHWDHIMGFPFFGPAYVPRTRIRVYGCHDILEQAFRLQQAPPCFPVAFDQLG
                     AAIEFVKLAPGQTREVSGLSVTPLLQRHSGDSFGFRFAFRGKSVVYSTDSEHKLEERA
                     ETERFVAFFRDADLVIFDAMYSLADAISVKADWGHSSNVIGVELCQMARARHLVLFHH
                     EPANNDTAIEGLLRDARRLEELTRGAQQLQISAAHDGLEIEL"
     gene            complement(1200..1574)
                     /locus_tag="VEL04_00285"
     CDS             complement(1200..1574)
                     /locus_tag="VEL04_00285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HYT46831.1"
                     /translation="MPLSHRNYGTARVLAPRGRLDHDSCEAFQADLAPHVEACERDGL
                     ALVLDLSGLEYVSSAGLRCLMIAAKQAATHHSRIVVASMQPVVSEIFQISRFSLVLEV
                     FPTMREALASVSPQAAAAFDRG"
     gene            1619..2431
                     /locus_tag="VEL04_00290"
     CDS             1619..2431
                     /locus_tag="VEL04_00290"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HYT46832.1"
                     /translation="MQKPKPGESPALRAARRLHHAHQQRERFRPLPEDLAPRSVQEAY
                     AIQDAFVALRAPKLGAIAGYKISLSSAEMRRFIGVDTPQSGVLLESTLQRSPGRVRAM
                     DHVRLIVEFEVAVEIGEDLPAADAPFFRERVARAVGAVMPALEIADDRGADYSQLAKH
                     PLELIADNGWNEGAVLGAPVKAWKEIDLAQVRGIASINGAVVGEGRGAAAMGHPFDAV
                     AWIADHLASLGRGLLRDEVVITGSLITSKAVKAGDRVKFALHGLGEVELRVD"
BASE COUNT          407 a          813 c          884 g          358 t
ORIGIN      
        1 atgagctgcg cgaccacggt gcgcggccag ggccattggc cgcgcgcgtc gagcgcgcgc
       61 tcgtcgatgg cgacgaccac tgccggcgcc gtagcgcggc tgcgcggcat cagcttctgg
      121 tagccgtcga acaccgcgtc gcgcaatgcc gcgagcggcg gcggctcgag cccgatcagc
      181 aggcagcaga tcgcgagcag gcccgtgccg agcgcgcgcg ggcccgatgc gaagctcaga
      241 gctcgatctc caggccgtcg tgagccgcgg aaatctgcag ttgctgcgcg ccgcgcgtca
      301 gctcctcgag ccggcgcgcg tccctcagca ggccttcgat cgccgtgtcg ttgttcgccg
      361 gctcgtgatg gaacagcacc aggtgcctgg cgcgtgccat ctggcaaagc tccacgccga
      421 tgacgttgct ggaatggccc cagtcggcct tgaccgagat ggcatcggcg agcgaataca
      481 tggcgtcgaa gatcaccagg tcggcgtcgc ggaagaaggc gacgaagcgc tcggtctccg
      541 cgcgctcctc cagcttgtgc tccgaatcgg tgctgtagac cacgctcttg ccccggaagg
      601 cgaagcgaaa gccgaaggaa tcgccggaat ggcgctgcag cagcggggtc acgctcaagc
      661 ccgaaacctc gcgcgtctgc ccgggtgcca gcttcacgaa ctcgatcgcg gcgccgagct
      721 ggtcgaaagc caccgggaag cagggcggcg cctgctgcag gcgaaacgcc tgctcgagga
      781 tgtcgtggca gccatagacg cggatgcgcg tgcgcggcac gtacgccggg ccgaaaaacg
      841 ggaagcccat gatgtggtcc cagtgcacgt gcgacatgaa gacgttgatg gtggccggcc
      901 tgccgccctg ccgcgcgagg acatgggcac cgaaggcgcg cgcgccgctc cccatgtcgc
      961 acacgaaata ctcctcgtgc ccggtctcga gctcgacgca ggacgagtgg ccgccgtagg
     1021 tgtggccgag cgaaaagggc agctcgcgct cggcgaacgc gtgcgcgtcg ccgtaggaag
     1081 cgaagcgctg gcccgaggcg gcgagcagcg cctgcgccag cttgtcgcgg atgtcagcgg
     1141 cggtcagtcc gaccgggatg gagccgcgcg tgccccagaa gcggacgcgc atgcgcccgt
     1201 cagccccggt cgaaagcggc tgccgcctgc ggcgataccg aggccagcgc ctcgcgcatc
     1261 gtcgggaaca cttccagcac caggctgaag cggctgatct ggaagatctc cgacacgacc
     1321 ggctgcatcg acgccaccac gatgcggctg tggtgcgtgg cagcctgctt ggctgcgatc
     1381 atcaggcagc gcaatcccgc gctggacaca tattcgaggc ccgaaaggtc gagcaccagg
     1441 gcgaggccgt cgcgctcgca ggcctcgacg tgcggcgcga ggtcggcctg gaacgcctcg
     1501 caactatcgt ggtcgaggcg gccgcggggc gccagcacgc gggcggtgcc gtagtttcga
     1561 tgggacaggg gcattgaggt cgttggaagc gccgttcggc ggtattctag ggtgccagat
     1621 gcagaaaccc aagccaggcg aatccccggc gttgcgcgcg gcgcggaggc tgcaccacgc
     1681 ccaccagcag cgcgagcgct tccgcccgct gccggaggat ctcgccccgc gcagcgtaca
     1741 ggaggcttac gcgatccagg acgcgttcgt tgcgctgcgc gcgcccaagc tcggggcgat
     1801 cgccggctac aagatttcgc tttcctcggc ggaaatgcgc cgctttatcg gggtcgacac
     1861 tccccagtcg ggtgtgctgc tcgagtcgac actccagcgt tcgccgggac gcgtgcgcgc
     1921 gatggaccac gtgcggctga tcgtcgaatt cgaggtcgcg gtcgagatcg gcgaggacct
     1981 gcctgcggcc gatgcgccct ttttccgcga gcgcgtcgcc agagcggtcg gcgcggtgat
     2041 gcccgcgctc gagatcgccg acgatcgcgg cgcggattac agccaactcg ccaagcatcc
     2101 tctcgagctg atcgccgata acggctggaa cgaaggcgcg gtgctcggcg caccggtgaa
     2161 ggcgtggaag gaaatcgatc tcgcccaggt gcgggggata gcgagcatca acggcgccgt
     2221 ggtcggcgag ggccgaggtg ccgccgccat gggtcatccg ttcgacgcgg tcgcctggat
     2281 cgccgaccac ctggcatcgc ttggccgcgg gctgctgcgc gatgaagtgg tgatcacggg
     2341 cagcctgatc acttccaagg cggtgaaggc gggcgaccgg gtgaaattcg cgctgcacgg
     2401 cctcggcgaa gtcgagctgc gcgtcgactg actgccgcgc gctcgtatcg aggcgctata
     2461 ga
//
LOCUS       DASPXW010000014         3059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_4269, whole genome shotgun sequence.
ACCESSION   DASPXW010000014 DASPXW010000000
VERSION     DASPXW010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4269"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..500)
                     /locus_tag="VEL04_00295"
     CDS             complement(183..500)
                     /locus_tag="VEL04_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT46833.1"
                     /translation="MTINSASVPFTQSLISQAPEASGVFALWQSGGVVYYGRASSGRA
                     TIRKALGEHYQGRAWSARRVTGCSWEVAADPERRLNELIREYELAHRCMPLWNDPQRL
                     PTD"
     gene            550..912
                     /locus_tag="VEL04_00300"
     CDS             550..912
                     /locus_tag="VEL04_00300"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYT46834.1"
                     /translation="MSSQILVVEDDESMRELLRMHLAAAGYEVRVAGDGIEAGYAVLQ
                     TTPDLIICDVSMPNMDGFQFIAALREDKSLPRIPVIFLTSREEGDARARELGASAFLT
                     KPVLADWLLTEVQRALSA"
     gene            917..2116
                     /locus_tag="VEL04_00305"
     CDS             917..2116
                     /locus_tag="VEL04_00305"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HYT46835.1"
                     /translation="MVSVDDIRAAAQRIAGAIEHTPCVHSHTLSRLTGAQLWLKFENL
                     QFTASFKERGALNKLLSLSAAERKRGVIAMSAGNHAQAVAYHAARLRIPAVIVMPRGS
                     PNTKVKNTRVHGAEVVLEGESLADAGRHAFERAARDNLVFVHPYEDPLVIAGQGSVGL
                     ELLAAVPDLDVLVVPIGGGGLIAGIATAAKALNGRIKIYGVESKAYASMYQRLNGLPI
                     QVGGDTIAEGIAVKDVGEISFSIISRLVEEILVVEEETIERAVVALIEIEKTVAEGAG
                     AAALAALLEHPGRFAGKRVGLPISGGNIDSRMLASVLMRGLVRDARLVRLRVTMPDVS
                     GSLAKVAGLIAAGGGNIVEVLHQRIFGTSSVRSPEVEFLIETRDRDHTDLLLQSLAAS
                     GVKVSAY"
     gene            complement(2097..2492)
                     /locus_tag="VEL04_00310"
     CDS             complement(2097..2492)
                     /locus_tag="VEL04_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYT46836.1"
                     /translation="MIDHIDHIVLTTRDKDACIRFYTEVLGMKLEKFLENRLALRFGR
                     QKINLHEWGREFEPKAHAPVPGSLDLCFIALVPLEVVIKKLESAKISLIQGPVAKTGA
                     MGPMRSVYVRDPDLNLIEISVYNLSTPKP"
     gene            complement(2518..2985)
                     /locus_tag="VEL04_00315"
     CDS             complement(2518..2985)
                     /locus_tag="VEL04_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HYT46837.1"
                     /translation="MSEAVPVVGRGFSFEDLRVGFRFRTHRRTIAESDLTAFINLTWF
                     TEELFAVEENAAGDASRAIKGRAVPGALVYAFAEGLLLPTMQDTGLAFLSATIDVKAP
                     TLVGDTLHVESEVTEHRLTSKGDRGLVRFANRVVNQRGEVVLEYNPLRLLKRK"
BASE COUNT          533 a         1007 c          987 g          532 t
ORIGIN      
        1 gtgcacccag gcggcaaccg ggtttgaata cgagatgagc gtaaacccgg ctcctttgcg
       61 tcaccggtat ctgtccgcct atgccaaaag aaatgccgga cagtggccgg agtgcaacga
      121 gaagaccaag cagacccctc ccgcaaactc aagcaccagg tagtacgacg caccaggaat
      181 ttctaatcgg tgggcagccg ctgcgggtcg ttccacagcg gcatgcagcg gtgcgcaagc
      241 tcgtattccc tgatcagctc gttcaagcga cgctcgggat cggcggcgac ttcccagctg
      301 cagccggtga cgcgccgcgc gctccaggcg cgcccctggt aatgctcgcc tagcgccttg
      361 cggatcgtcg cccggccgga cgaagctctg ccgtaataca ccacgccgcc gctctgccaa
      421 agcgcgaaca cgccgctcgc ctccggcgcc tgcgagatca gggactgcgt aaaaggtacg
      481 ctcgcgctat tgatcgtcat ggcccgtttc ccttgctgaa gcggcgaatg ataaacctat
      541 aatgcggccg tgagctcgca gatcctggtg gtcgaggacg acgagagcat gcgcgaattg
      601 ctgcgcatgc atctcgcggc tgccggctat gaggtgcggg ttgccgggga cggcatcgag
      661 gcgggctacg ccgtgctgca gacgacgccc gacctcatca tctgcgacgt ctcgatgccg
      721 aacatggacg gcttccagtt catcgctgcg ctgcgcgaag acaaatcgct gccgagaatt
      781 ccggtgatct tcctcacctc gcgggaagaa ggcgatgcgc gcgcgcgcga gctgggcgcg
      841 agcgccttcc tcaccaagcc ggtgctcgcc gactggctgc tcaccgaggt gcagcgcgcg
      901 ctctccgcat agcccggtgg tttccgtcga tgacatccgc gccgccgcgc agcgcatcgc
      961 cggcgcgatc gagcatacgc cgtgcgtcca cagccacacc ctttccaggc tcactggcgc
     1021 gcagctctgg ctcaagttcg agaacctgca gttcaccgcc tcgttcaagg agcgcggcgc
     1081 gctcaacaag ctgctttcgc tctccgcggc cgagcgcaag cgcggcgtga tcgcgatgag
     1141 cgccggcaac catgcccagg cggtcgccta ccacgccgcg cgcctgcgca ttccggcggt
     1201 gatcgtgatg cccaggggct cgcccaacac caaggtgaag aacacccgcg tgcacggcgc
     1261 ggaggtagtg ctcgagggcg aatcgctcgc cgacgccggc cgccatgcct tcgagcgcgc
     1321 cgcgcgcgac aacctcgtgt tcgtgcatcc ctacgaggat ccgctggtga tcgcgggcca
     1381 gggaagcgtc ggcctcgagc tgctcgcggc ggtgccggac ctcgatgtgc tggtggtgcc
     1441 gatcggcggc ggcgggctga tagcgggcat tgcgacggcg gcaaaggctt tgaacggcag
     1501 gataaaaatc tacggcgtcg aatcgaaagc ctatgcctcg atgtaccagc ggctgaacgg
     1561 cctgccgatc caggtcggcg gcgacaccat cgccgagggc atcgcggtga aggatgtcgg
     1621 cgagatcagc ttttcgatca tcagcaggct ggtggaagag attctggtgg tcgaagagga
     1681 aaccatcgag cgcgcggtgg tcgcgctgat cgagatcgag aagacggtcg ccgaaggcgc
     1741 cggcgccgcg gcgctcgccg cgctgctcga gcaccccggg cgcttcgccg gcaagcgcgt
     1801 cggcctgccg atctcgggcg gcaacatcga cagccgcatg ctcgcctcgg tgctgatgcg
     1861 cggcctggtg cgcgacgcgc gtctcgtccg gctgcgcgtc accatgcccg acgtctcggg
     1921 cagcctcgcc aaggtcgccg gcctgatcgc cgccggcggc ggcaatatcg tcgaggtgct
     1981 gcaccagcgc atcttcggca cttcctcggt gcgcagcccc gaggtcgagt tcctgatcga
     2041 aacccgcgac cgcgaccaca ccgacctgct gcttcagtcg ctggccgcga gcggcgtcaa
     2101 ggtttcggcg tactgaggtt gtagaccgag atctcgatca gattgaggtc cgggtcacgc
     2161 acgtataccg agcgcatcgg ccccatcgcg ccagtcttcg ccaccggccc ttggataagg
     2221 ctgatttttg cagattccaa tttttttatt acgacctcga gaggcaccaa cgcgatgaag
     2281 cataaatcca gggatcccgg cacgggcgcg tgcgccttcg gctcgaactc cctgccccac
     2341 tcgtgcaggt tgatcttctg acggccgaag cgcaaagcaa gtcggttttc cagaaacttc
     2401 tccagcttca tgccgagcac ttcggtgtag aagcggatgc acgcgtcctt gtcgcgcgtc
     2461 gtgagaacga tgtggtcgat gtggtcgatc atgggaaacg tggtcggcgc ctgattttca
     2521 ctttcgcttc aagagccgca gcgggttgta ctcgagcacc acctcgccgc gctggttgac
     2581 caccctgttc gcgaagcgca ccaggccgcg gtcacccttg gaggtcaggc gatgctcggt
     2641 gacttcggac tcgacgtgca gcgtatcgcc cactagcgtc ggcgccttca catcgatggt
     2701 ggcgctgagg aaggcgagcc cggtgtcctg catcgtcggc agcagcagcc cctcggcaaa
     2761 ggcatagacc agggcgccag gcaccgcgcg gcccttgatc gcgcgcgacg catcgcccgc
     2821 ggcgttttcc tcgaccgcga acagctcctc ggtgaaccag gtgaggttga tgaacgcggt
     2881 gagatcggat tcggcgatgg tgcgccggtg ggtgcggaag cggaagccga cgcgcaggtc
     2941 ctcgaacgag aagcctcgtc cgacgacagg taccgcttcg ctcacttctt gtcgaacctg
     3001 gagcgcgcct ggttgaaggc ctcctgcatc ttcttccacg cgtcatcggt gccgcgcat
//
LOCUS       DASPXW010000015         4007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_508817, whole genome shotgun sequence.
ACCESSION   DASPXW010000015 DASPXW010000000
VERSION     DASPXW010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508817"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2073
                     /locus_tag="VEL04_00320"
     CDS             <1..2073
                     /locus_tag="VEL04_00320"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HYT46838.1"
                     /translation="GAERRLSEAKARLELALDGGNLAEWHYVVDSDELYAGDGWVRFL
                     GHDRSPPVRLGLRLFEMMHAEDRERTKAAWVRALKGEVPEFDAEFRTQTRGGEWKWLH
                     ARGRVSERAADGRALRMSGTVADIDTHKRAEQAGAERDQRFQDVAATSGEYVWETDPE
                     GRYSYLSERVEAVLGYARAELLGRAPSEFMPVGEDAALPRDGDSPVRDLVHRMITKSG
                     SVVWQSVSAVPMRDATGRWRGYRGTAADVTARKSAEARIEQLAMRDALTGLPNRALFA
                     ERAGQAILAAARGRTELALLTVDLDRFRLVNESLGHQAGDALLRAVAERLQGTLRRED
                     LSRIGGDAFVLLVHGLKSAEEAAMVARRLLSVLARPFNLEGQPLTAGASVGIALYPKD
                     GRDFVELLKNADAALYHAKESGRGTFSFFEPQLGARATERLKVENELRGALARNELVL
                     HWQPVVRGRSEIVGAEALVRWRHPSRGLLLPEQFVPLAEDCGLIGAVGEWTLERALSQ
                     AGAWQRTLRSPLWFALNVCAPELAQGQGFVEKLAASLKANALEGARIELEVTERVLMS
                     QLAGNVETMRRIGELGVRFAIDDFGTGYSSLAYLRQLPIDKLKVDRAFLRAIDTEPAD
                     EAIVRTIASLAKTLGITVAAEGVERQGQLERLLALGCDEWQGHYLSPPLAPAEFEHLL
                     TRAARRAS"
     gene            complement(2083..3063)
                     /locus_tag="VEL04_00325"
     CDS             complement(2083..3063)
                     /locus_tag="VEL04_00325"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017238.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYT46839.1"
                     /translation="MSAGMVYLGTQAGLFRAMQGKGAMRLDAVVRDSGLQPRYVEEWL
                     KGMASAGYLEYDPKAQTFTLPEEHAYFLASEGSDHFVGGLFAMVPPLLRIAPQVARAF
                     VEGGGVAFGEFGPDCVDALDLINRGQYEQRFADYWLKALPEARERLAAGGRALDVGCG
                     SGRVCIALAKAFPAAEVVGIDPDAESIRRAKAAGAKNIRFHVQTTSEVKRGSGFDLIT
                     LCDALHDLAAPLAVLREIRALLKPDGTLFIIEPRAADRLEDNRNAVATTFYGFSLFHC
                     MTQSLACGGPGLGTCLGPAATEKLVREGGFKRFEKLDIKSLTNLFYAARP"
     gene            complement(3129..3869)
                     /locus_tag="VEL04_00330"
     CDS             complement(3129..3869)
                     /locus_tag="VEL04_00330"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HYT46840.1"
                     /translation="MYWRQGCSSCVKVKEFLTGLGVEFESIDVGARPQAMDELREMGV
                     RTVPVVARGREYVFAQALEDVSRFVGRDFVPQRLAPEALVKKWLKLLAAAQRHALQLP
                     RERLGERATPGRDRSIRDLAYHVYQVPEAFLEAVEHGVEDLTAVYNAPPPANVTTVGQ
                     ISVYGKGISERLKGWWDRLPDKSCRQTVKTYYGVRSQHELLERCAWHSAQHARQIIAV
                     LGGFGIAPETPLTAEDYAGLPMPVGLWE"
BASE COUNT          602 a         1404 c         1407 g          594 t
ORIGIN      
        1 ggcgccgagc ggcggctgtc cgaggccaag gcgcgcctcg agctcgcgct cgatggcggc
       61 aacctcgccg agtggcatta cgtcgtcgac agtgacgagc tctacgccgg cgacggctgg
      121 gtgcgcttcc tcggccacga ccgctcgccg ccggtcaggc tgggcctgcg gctcttcgag
      181 atgatgcacg ctgaagaccg cgaacggacc aaggccgcgt gggtgcgcgc gctcaagggc
      241 gaggtgccgg aattcgacgc cgagttccgc acgcagacgc gcggcggcga gtggaaatgg
      301 ttgcacgcgc gcggccgcgt cagcgagcgc gccgccgacg ggcgcgcgct gcgcatgtcg
      361 ggcacggtcg ccgatatcga tacccacaag cgcgcggagc aggcaggcgc cgagcgcgac
      421 cagcgcttcc aggacgtcgc cgcgacctcc ggcgagtacg tctgggaaac cgaccccgag
      481 gggcgctaca gctatctctc cgagcgcgtc gaggcggtgc tcggctacgc gcgcgcggag
      541 ctcctcggcc gcgcgccttc cgagttcatg ccggtgggcg aggatgcggc gctgccccgg
      601 gatggagaca gcccggtgcg cgacctggtg caccgcatga tcaccaagtc gggaagcgtg
      661 gtctggcagt cggtgagcgc ggtgccgatg cgcgacgcca ccggtcgctg gcggggctac
      721 cgcggcaccg ccgccgacgt cacggcgcgc aagagcgccg aggcgcgcat cgagcagctc
      781 gcgatgcgcg atgccctgac cggccttccc aaccgggcgc tgttcgccga gcgcgccggc
      841 caggcgatcc tcgccgccgc gcgcggccgc accgagctcg cgctcctcac ggtcgatctc
      901 gaccgcttca ggctggtcaa cgaatccctc ggccaccagg ccggcgatgc gctgctgcgc
      961 gcggtggccg agcggctgca gggaacgctg cgccgcgagg acctgtcgcg catcggcggc
     1021 gacgcgttcg tgctgctggt ccacggcctc aagtcggccg aggaggcggc gatggtcgcg
     1081 cggcgcctcc tgtcggtgct ggcgcggccg ttcaacctcg agggccagcc gctcaccgcc
     1141 ggcgcctcgg tcggcatcgc gctctatccg aaggacgggc gcgacttcgt cgagctgctg
     1201 aagaacgccg acgccgcgct gtaccacgcc aaggaaagcg gccgcggcac gttcagcttc
     1261 ttcgagccgc agctcggcgc gcgcgccacc gagcgcctca aggtggagaa cgagctgcgc
     1321 ggcgcgctcg cgcgcaacga gctggtgctg cactggcagc cggtggtgcg cgggcgcagc
     1381 gagatcgtcg gcgccgaagc gctggtgcgc tggcgccatc ccagccgcgg cctgctgctg
     1441 cccgagcagt tcgtgccgct cgccgaggac tgcggcctga tcggcgcggt gggcgagtgg
     1501 acgctggagc gggcgctgtc ccaggccggc gcctggcagc gcacgctgcg ctcccccctg
     1561 tggttcgcgc tcaacgtctg cgcgcccgag ctcgcgcagg gccagggctt cgtcgagaag
     1621 ctcgcggcct cgctcaaggc gaacgcgctc gagggcgcgc gcatcgagct cgaggtcacc
     1681 gagcgcgtgc tgatgtcgca gctcgccggc aacgtcgaga ccatgcgccg catcggcgag
     1741 ctcggcgtgc gcttcgcgat cgacgacttc ggcaccggct attcgagcct cgcctacctg
     1801 cgacagctgc cgatcgacaa gctgaaggtc gaccgcgcgt tcctgcgcgc catcgacacg
     1861 gaacccgccg acgaggcgat cgtgcgcacc atcgcctcgc tcgccaagac cctgggcatc
     1921 acggtcgccg ccgagggggt ggaaaggcag gggcagctcg agcgcctgct ggcgctgggc
     1981 tgcgatgaat ggcaggggca ttacctcagc ccgccgctcg cgccggcgga gttcgagcac
     2041 ctgctcacgc gcgccgcccg ccgcgcgtcc tgaacgcgtg cttcagggcc gcgcggcgta
     2101 gaagaggttg gtgaggctct tgatgtcgag cttctcgaag cgcttgaagc cgccttcgcg
     2161 caccagcttc tcggtggccg cggggccgag gcaggtgccg aggcccgggc cgccgcacgc
     2221 gagcgactgc gtcatgcagt ggaacaggct gaagccgtag aaggtcgtgg ccaccgcatt
     2281 gcggttgtcc tcgagccggt cggcagccct gggctcgatg atgaacagcg tgccgtcggg
     2341 cttgagcagg gcgcgaatct ctctcagcac ggcgagcggc gcggcgagat cgtgcagtgc
     2401 atcgcatagc gtgatgaggt cgaagcctga tccgcgcttc acttccgacg ttgtttgaac
     2461 atgaaaccgg atattttttg cgccggcggc cttcgcgcgg cgaatcgatt ctgcgtccgg
     2521 atcgatgccg acgacctcgg cggcggggaa agccttggcg agcgcgatgc agacgcggcc
     2581 cgagccgcag ccgacatcga gcgcgcgccc gcccgccgcg aggcgttcgc gcgcctcggg
     2641 cagcgccttc agccagtaat cggcgaagcg ctgctcgtac tgcccgcggt tgatgagatc
     2701 gagcgcgtcg acgcaatcgg gcccgaactc gccgaaggcg acgccgccgc cttcgacaaa
     2761 ggcgcgcgcc acctgaggcg cgatgcgcag gagcggcggc accatggcga acaggccgcc
     2821 gacgaaatgg tcggagcctt ccgaggcgag gaaatacgcg tgctcctcgg gcagcgtgaa
     2881 agtctgcgcc tttgggtcgt actcaaggta gccggcgctc gccatgccct tgagccattc
     2941 ctcgacgtag cgcggctgca ggcccgagtc gcgcaccact gcgtcgaggc gcatcgcgcc
     3001 cttgccctgc atggcgcgga acaggcccgc ctgcgtgccc aagtacacca tgcccgcgct
     3061 catggcgccc gccatatcgc ggaacacccg gtcgagcgcc tgtcttgtct tctccttgtc
     3121 cagccgggct actcccagag gccgaccggc ataggcaggc cggcatagtc ctcggcggta
     3181 agcggggtct ccggcgcgat gccgaagccc ccgagcaccg cgatgatctg gcgcgcgtgc
     3241 tgcgccgaat gccaggcgca gcgctcgagc agctcgtgct gggagcgcac gccgtagtag
     3301 gttttcaccg tctgccggca ggatttgtca ggcagccggt cccaccagcc cttgagcctt
     3361 tcacttattc cttttccata gacagaaatc tgcccgaccg tagtcacgtt cgccggaggg
     3421 ggcgcgttgt acaccgcggt gaggtcctcg acgccgtgct cgaccgcctc gaggaaggct
     3481 tcgggcacct ggtagacgtg ataggcgagg tcgcgaatgc tgcgatcgcg cccgggagtg
     3541 gcgcgctcgc cgagccgctc gcgcggcagc tgcagggcgt ggcgctgcgc cgcggcgagc
     3601 agcttgagcc atttcttcac cagcgcctcg ggcgcgaggc gctgcggcac gaaatcgcga
     3661 ccgacgaagc gcgacacatc ctcgagcgcc tgcgcgaaca cgtattccct gccgcgcgcc
     3721 acgaccggca cggtgcgcac gcccatctcg cgcagctcgt ccatcgcctg cggccgcgcg
     3781 ccgacgtcga tcgactcgaa ctccacgccg aggccggtga ggaactcctt caccttgacg
     3841 cagctcgagc agccttgccg ccagtacacc cgcagatttt ccattggatc agggcgcgac
     3901 agagacgccg cgcaccccgt tgctgtttat aagtttttta ataaaacctg actttttcat
     3961 atcgtcggcg aacgcagaca ggtatttcgc cgccgcggca cgcgcct
//
LOCUS       DASPXW010000016        12035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1518783, whole genome shotgun sequence.
ACCESSION   DASPXW010000016 DASPXW010000000
VERSION     DASPXW010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12035
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1518783"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1126
                     /locus_tag="VEL04_00335"
     CDS             <1..1126
                     /locus_tag="VEL04_00335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VCBS domain-containing protein"
                     /protein_id="HYT46841.1"
                     /translation="PDDATAFVPQTNVAGANGTFSVAADGTWTYTANSAFDNLNVGQS
                     VSDTFTVASADGTTTTVQVTITGTDHLVVFSSQTTPGVLASASFPAAAAGLVPTPESA
                     APAAQYAAAPLGLPVDRSVAASNLAAQLTVAAVRWPDGTFVGADAGSSTVAGGFPVVR
                     LLPEQAGIMRMSDLDDIGKVTGGGQRLFAYRGIPDMRLLGGGNDSLAVPQDAFAHTDP
                     HAIVILEARLANGAPLPSWLSFEGTRGEFAGTPPEGLQGSMEVEVIARDTEGREAHAV
                     FVLQVEGLRAAAGEPVDPAGAALGLDVDKKEAEKARLEAARQATEGRGPVKPGTGAEP
                     KPQKGPAASFSDHLRNANAVRDPLLDRIVRGGRDTPGTRR"
     gene            1403..3244
                     /locus_tag="VEL04_00340"
     CDS             1403..3244
                     /locus_tag="VEL04_00340"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HYT46842.1"
                     /translation="MARAIKYQAEHRLRRMEEAASEAQLDVAKFDLLPKLMLNAGYSW
                     RNNEAFGFGFTPSGTIATTPTASQERTHTTSSIGLAWNLLDFGTSYFRARQLADQKLI
                     AEERRRKAVQTLMHDVRVAWWRAEAAERLLPAAERLLAEIEQAIDKTRYIEARRLLPP
                     VQTATLRRALLDLNQQISLRRQDLAQSRIELAALVNAAPGSELRVASPDSDAREVPDL
                     TADLEKLEILALQSRPEIAEEGYRARISADEARKALVALLPNLSLDLARNRDSNKFLL
                     NNVWTSAGVNVAFNLVKVFSLPAQNRSEEATRRADDARRHAMAMAVLTQTRIAAVRYT
                     LVADEFLVWNEAARDDDLIVQYLSSSEKVGIDTELELIRARARAMASHMNRDLSYANL
                     QATVARLFNSVGYDAVPREDEATAIAELSGRVQARFVELERATFTERAAAKKPALIPG
                     NVSGAEPRVAALLRQGIDRVLGSAGMKAADAAAADARLDLQLSVGLAGEGRRPVSIGI
                     SVVPAAAASASLRREFRTTLSEPVDDEQWRVLGEGAAYRVIGDIAPARITRPALRMQQ
                     SLHIPSPGKRVEGRNIFPGRTVEPLELRLDRLFGSVVKDGAGGDGSQ"
     gene            3495..4196
                     /locus_tag="VEL04_00345"
     CDS             3495..4196
                     /locus_tag="VEL04_00345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HYT46843.1"
                     /translation="MLTPAEKPKPAAAEIAALIVAGEETTLSSQMAGKIRRVRVGLGD
                     QVEKGAPLVEFDCLEQSAQLETANAEYRAARETHLARLRLQALGAAGELEVTVAAASA
                     DKARSQVAVRRSQLAYCSVDAPFAGNVARLRVKTAESVNLGQPLVDLVNPASLKAQMF
                     VPVAWIAWLQPGTPLSIRVSETGQTFRARVAKVNSRVDGVSQQLELEAHIEKGQGRLL
                     PGMVGSATFERPPAK"
     gene            4206..5543
                     /locus_tag="VEL04_00350"
     CDS             4206..5543
                     /locus_tag="VEL04_00350"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HYT46844.1"
                     /translation="MSDSPHPALALLEFERRIQSAASNREVAFRAVNDGSQVLRFDQA
                     VLWRLDVFSRPLVAAASGLADVSVDSPYQQWLVRLIQTITPASFEASRTLTAAELPEA
                     IAEDGADWCPAHILHCPLRGPNDAALGGILFFRAQPFTEAEHAAAEWIARSTGYALWA
                     WRSERSGIKRWLKSRKTWRQLAVAAGLAALACLIPVRLSVLAPAEITSARPIPVTSPL
                     DGAVRDILVKPNQIVKADELLVVLDDTGLRNRLELASRALDIARADLQRATFKSFSDE
                     ASRFELQVLNARTQEKLAEVTYLTELLEKSKLTAPQGGIAVFSSQEDWRGRPVQVGER
                     IMMIADPSLIDVTIYVPPEDAVELERGSEVNLLLHVDPLSPLDARIERSSYEATVTPD
                     GTLAYVVRAQLLPGQGLPRIGLRGTAKIYADRVTLGYYLLRKPLAFLRRSLGI"
     gene            5654..6511
                     /locus_tag="VEL04_00355"
     CDS             5654..6511
                     /locus_tag="VEL04_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HYT46845.1"
                     /translation="MTPFELTEPKSLREAIELLDPDDTAVRPMSGGTALMLMMKAGVL
                     RPRRLVNLKKIGLAEIGKGPAGELRIGAMARLSDIERSPIVRKDCPVIARTLLTLSNV
                     RVRNVATLGGHLAHADPHMDLPPVLSALGARATIAGPAGERTIPVEEISSGYLATTLR
                     LNELITAIEVPPLGGRRAAYMKCTTRSADDWPALGVAVVLDFQKDVLQTASLVISAAT
                     DKPTRLRSAEKLLRGKTLDESSLKQAGEAAAAEVQMESDMHGSAAYKKQLLRVYLGRA
                     IHEASSGRH"
     gene            6486..8759
                     /locus_tag="VEL04_00360"
     CDS             6486..8759
                     /locus_tag="VEL04_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007744060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HYT46846.1"
                     /translation="MKRAAGATETPQVGRSVPRLESWLKVTGRAEYVHNLRLPGMLYG
                     KIFRSSVAHGRIKRIDVSVAQAVGGVHRVVIAADLKEIVPDPFYGPAFHDQPILAVDK
                     VRYVGEPVAVVLAADPHVAEEAVHLITAEYEELPAVYDEVEAMTSKAIVHDALKPAGT
                     FPDLKHLKGRTNTNIALDFHLRRGDAARALGDAHRVFEHRFRTQQVMHTPLEPLVSVA
                     ESTENSLTIHTASQSPSFVRIEIARLLGWPENKVRVRVPFLGGGFGAKLYIKLEALVS
                     VLSLLTKKPVKVSLTMEEQFFTITKHASTFRIKSGVTRDGRIVARECEVWWNGGAYAD
                     IGPRVTQKSGFTAPGPYDIEHVAIDSYALYTNLPPAGALRGFGIPQLVWAYESHTDMM
                     ARELGIDPLEFRRMNLLRDGRPQATGTVMRDAAIEKVLDRLESLMDWETPWDKGEGSV
                     RRGRGIAIGFKACVSPTSSLAAVSVNADGGCTVYCSTVDMGQGSDTAMAQIAGEALHL
                     PAESVTVMHSDTDVTPYDMATLGSRSLFHMGNAVKLAAEEAGRKLQALREELKLPADA
                     EIPDIFRKKYGMQAGSVLGTGSFIPSYAAPDHATGQTPNATPFWMVGGTGVELEVDTE
                     TGHVRIARLVNVADVGTPINPRIVETQLSGAAIMQLGFTLFEKMVFDADGQLRNASLA
                     EYKIPGLLDLPREIINEAVTAAQRSGPFGAKGVGETGTFGVSPAIANAIHDAVGVRVT
                     SLPLNPETIFIGLQKLR"
     gene            8756..9217
                     /locus_tag="VEL04_00365"
     CDS             8756..9217
                     /locus_tag="VEL04_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007744061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HYT46847.1"
                     /translation="MRRAIQFRLNGEAVEAQVAPHESLVEVLRDAFGLYGARESCGQG
                     LCGCCTVLVDGRAVSGCLYLASFAAGAEVSTVEAGKELDAVQEAFTERAAFQCGFCTP
                     GFILMTRQLLDENPDPDDDAIRHYLSGNLCRCAAYPEIIEAVKLAARKRRK"
     gene            9217..10173
                     /locus_tag="VEL04_00370"
     CDS             9217..10173
                     /locus_tag="VEL04_00370"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYT46848.1"
                     /translation="MKLLLAALALAASLAQAQDYPNKPIHMLVPFAPGGAVDTTARVL
                     AQAMQQRVKWQFVIENRPGGNGFIATTAVAKSPADGYTLLMAHTGEFAVNPALFDNIP
                     YELERDFVPITMVSDSPMLYLANAQAPFNTFQELVAQAKAKPGAISFGSAGNGSINHL
                     AGEWLAQAAGIQLLHVPYKGGAPAAAAVAAGDVHFGVMAVPGAMPHIKSGRGKVIGIT
                     TAKKSPLDPSWATPRDAGIPDLDASIWVGLFAPKGTPQAVVDRINKEVRQTLAMPDVQ
                     KRFIESAGGEAVGMTPAEFLARIKSDAERYKRVVQKAGVKPG"
     gene            complement(10160..10750)
                     /locus_tag="VEL04_00375"
     CDS             complement(10160..10750)
                     /locus_tag="VEL04_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT46849.1"
                     /translation="MRLIAFLEYVAVIIGSVGMVAGQYFGLAKGFHLGVFVAGAGFAL
                     AGIDAIATRRMCFRPSDDVYANYAGAPALIVGMMVLVVGAGMIAAAYLLDNDQWQSTV
                     NYLMRRPAPLLGTGGLFVIGIGVLMMLNPQGRSSWIWRILVYFPRALLGLLVAAAGIA
                     IIGLGVWEWLEPQAFRAFVGTLPKRLDQLLHWISRA"
     gene            complement(10800..11918)
                     /gene="dnaJ"
                     /locus_tag="VEL04_00380"
     CDS             complement(10800..11918)
                     /gene="dnaJ"
                     /locus_tag="VEL04_00380"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006417099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HYT46850.1"
                     /translation="MAKRDYYEVLGVNRDAADGDLKNAYRKLAMKWHPDRNPENPKAE
                     ERFKEAKEAYEILSDAGKRAAYDQFGHAGVDPHAAGAAAGSMGGFGDIFSDIFGEIFG
                     GGRSGRSTVFRGADLRYNLEITLEQAAHGFETKIRIPAMAACDICKGTGARPGTQPAT
                     CPTCRGAGQVRVSQGPFSIAQTCPRCHGSGSVIPNPCAQCSGSGRVKLQKTLSVKIPA
                     GVDQGDRVRLSGEGEPGVNGGPPGDLYVQVHIKPHPVFTRDHDDLHCEMPISITAAAL
                     GGEISIPTLDGSAKIRVPAETQTGKTFRLKGKGIKGVRSQAHGDLFCHVVVETPVSLT
                     ERQRQLLREFDAITQQDSARHNPRAKGWFDKVKEFFES"
BASE COUNT         2060 a         3964 c         4051 g         1960 t
ORIGIN      
        1 tccggacgat gcgacggcgt tcgtacccca gaccaacgtg gccggggcga acggcacgtt
       61 ctccgttgca gcggacggta cctggaccta caccgcgaat tccgctttcg acaacctgaa
      121 cgtcgggcaa tcggtgtcgg atacgttcac cgtggcttcg gcagacggca ccaccacgac
      181 ggtccaggtg acgattaccg ggaccgatca cctggtggtg ttctcgtcac agacaacgcc
      241 gggcgttcta gcgagcgcat cgtttcccgc cgcggcagcg ggtcttgtcc cgacgccgga
      301 gtctgcagcc cctgcggcgc agtacgcggc agcgcccctc gggctgccgg tggatcgctc
      361 cgttgcggct tcgaaccttg cggcgcaact cacggtggcg gcagtccggt ggcccgatgg
      421 caccttcgtc ggcgcggacg ccggcagctc gaccgtcgct ggcggcttcc cggtcgtccg
      481 actgctgccg gaacaggccg gcatcatgcg catgagcgac ctggacgata tcggcaaggt
      541 caccggtggt ggtcagcgcc ttttcgcgta tcgcggcatt cccgacatgc ggctgctcgg
      601 cggcggcaac gattcgctcg cggtgccgca ggatgccttc gcccataccg acccgcacgc
      661 catcgtgatt cttgaagcgc gcctcgccaa cggcgcgcct cttccctcct ggctcagctt
      721 cgagggcacg cgaggcgagt tcgccggcac ccctcccgag ggcctgcagg gcagcatgga
      781 agtcgaggtc attgcccgcg acaccgaggg gcgcgaggcg cacgccgtgt tcgtgctgca
      841 ggtcgagggg ctgcgggcgg ccgcaggcga gccggtcgac ccggccggcg cagcgctagg
      901 cctggacgtg gataagaagg aggccgagaa ggcccgtctc gaggcggcac ggcaggcgac
      961 cgaaggacgc ggcccggtca aacccggcac cggggccgaa cccaagccgc agaaggggcc
     1021 agcggcgtcg ttcagcgacc acctgcgcaa cgcgaacgca gtgcgtgacc ccctgctcga
     1081 tcgcatcgtg cggggcggcc gagatacgcc cggcacacgc cgctagagca gcttttccta
     1141 gctcggttaa actagcgtct tagcgaccgc gcgtaaaccg gcgcgccaaa acgagaatat
     1201 gcagtttccg agccgatcat tgatgctatc aggatgggcg aagacagtgc ttgcggctgc
     1261 tcttagcgcg ctgctttacg cctgtgccgt gacgccgcgt ccgatgaatg ccgaagagcg
     1321 cgcgaagctc gccgctgacg cgcagcggca gatgttcgaa ggccaggagc cgctggaaga
     1381 gccgctcacg ctttctcagg cggtggcgcg cgccatcaag tatcaggccg agcatcgcct
     1441 aaggcgcatg gaggaagcgg cgtcggaggc gcagctcgac gtggcgaagt tcgacttgct
     1501 gccgaagcta atgctcaatg cgggctatag ctggcgcaac aacgaggcgt tcggcttcgg
     1561 cttcacgccg agcgggacga ttgccaccac gccgaccgct tcgcaggagc gcacgcacac
     1621 tacgtcgagc atcggcctcg cgtggaacct gctcgacttt ggcaccagct atttccgcgc
     1681 gcgccagctt gccgatcaga agctcatcgc cgaggagcgc cggcgcaagg cggtccagac
     1741 gctgatgcac gacgtgcgcg tcgcgtggtg gcgcgccgag gccgcggagc gcctgttgcc
     1801 ggcagccgaa cgcctgctcg cggagatcga gcaggcgatc gataagacgc gctacatcga
     1861 ggcgcgcagg ctgctgccgc cggtgcagac ggcgaccctg cgccgcgcgc tgctcgacct
     1921 caaccagcag atttccctgc gccgccagga cctcgcccaa tcgagaatcg agctggccgc
     1981 actggtcaac gctgcgccgg gcagcgagtt gcgtgtggca tcgccggaca gcgacgcgcg
     2041 cgaagttccg gacctgaccg ctgacctgga aaagctggag atcctcgcgc ttcagtcgcg
     2101 ccccgaaatc gccgaggaag gctatcgcgc ccggatctcc gccgatgagg cgcgcaaggc
     2161 gctggtggcg ctgctgccga atctgagcct cgatctcgcg cgcaatcgag acagcaacaa
     2221 gtttctcctg aacaacgtct ggacctcggc cggcgtgaac gtagccttca atctggtgaa
     2281 ggtgttttcg ctgccggcgc agaaccggtc cgaggaggcc acgcggcggg cggacgacgc
     2341 gcgtcgccac gcgatggcga tggcggtgct gacccagacg cgcatagcgg cggtccgcta
     2401 cacgttggtc gccgacgagt tcctcgtatg gaatgaggcg gcgcgcgacg acgatctgat
     2461 cgtccagtat ctgtcgtcga gcgaaaaggt cggaattgac actgagctcg agctgatccg
     2521 cgcccgcgcg cgcgccatgg ctagccacat gaaccgcgac ctgtcctacg ccaacctgca
     2581 ggccactgtt gcgcggcttt tcaactccgt tgggtacgac gcggtgcccc gggaggacga
     2641 agcgacggcc attgccgagc tatcgggccg cgtgcaagcg cgctttgtcg aactcgagcg
     2701 cgcgaccttc accgagcgcg ctgcggcgaa gaagcccgcg ctcatcccgg gcaacgtttc
     2761 cggcgcggag ccgcgcgttg cggccttgct gcgccagggc atcgaccggg tgctgggcag
     2821 cgccggcatg aaagccgctg acgcggctgc ggccgacgcg cggctggatc tgcagctctc
     2881 cgtcgggctg gccggagaag ggcgcaggcc ggtctccatc ggcatcagcg tggtgccggc
     2941 tgccgcggcc agcgccagcc tcagacgcga gttcaggacg acgctgagcg agccggtgga
     3001 cgacgagcag tggcgcgttc tgggcgaagg tgcggcttat cgcgtgatcg gcgatatcgc
     3061 gccggcgcgc atcacgcggc cggctctgcg catgcagcaa tctctgcata tcccgtctcc
     3121 gggaaaacgc gtggaaggtc gcaacatttt tcctgggcga accgtggaac cgctcgagct
     3181 acgcctggat cggcttttcg gctccgtggt caaggacggc gcgggtgggg atggcagcca
     3241 atgaaacagc gtctaatttg cggggtggct cagcaatgac actgtcccgg cgcgagtggt
     3301 tctggcgcgt attcgcagcc ctgatggtcg tcgaaatctg ctggtttgca tgggtcgcgt
     3361 accatatgtg gccgcggcag ctggcaacgg acttggcgta taaggcggaa atcgaagcgc
     3421 agtctcgccc gcaaagagtt cccgcggcgc cgccacctcc tgcggcaccg gcacttgcgc
     3481 ttaagactga tacggtgctt acccctgccg agaaaccgaa accggctgcc gcggaaatcg
     3541 cggcgctcat cgtcgccggc gaagaaacca cgctgtccag ccagatggcc ggcaagatcc
     3601 gccgcgtccg cgtggggctc ggcgaccagg tcgagaaagg cgcgcccctg gtggagttcg
     3661 attgcttgga gcaatcggcc cagctcgaga ccgcgaatgc ggagtatcgc gcggcgcgcg
     3721 agacgcacct cgctcgtctt cggctgcagg cgctcggcgc ggctggcgag ctcgaggtaa
     3781 cagttgccgc ggcgtctgcg gacaaggcgc gcagccaggt agcggtgcgt cgttcacagc
     3841 tcgcctattg cagtgtcgac gcaccctttg ccggcaatgt cgcgcgcctg cgcgtcaaga
     3901 ccgcggaaag cgtaaacctg ggccagccgc tcgtcgacct cgtcaatccc gcttccctga
     3961 aggcgcaaat gttcgttccg gtcgcctgga ttgcgtggtt acagccgggc acgcccctga
     4021 gcatccgcgt cagcgaaacc gggcagactt tcagggccag ggttgccaag gtgaactcgc
     4081 gtgtcgacgg cgtcagccag cagctcgagc tcgaggcgca catcgaaaag ggccagggca
     4141 ggctactgcc cggaatggtg ggctccgcca cattcgagcg gccgcctgcg aagtaagtgc
     4201 agccgatgag cgacagcccc catcctgccc tggcgttgct cgaattcgag cgccgtatcc
     4261 agtccgccgc ttccaaccgg gaagtggcgt ttcgcgcggt caacgatgga tcgcaggtgc
     4321 tgcgcttcga tcaggcggtg ctctggaggc tggacgtttt ttcgcgcccg cttgtcgcgg
     4381 cggcctcggg acttgccgac gtttcggtgg acagtcccta tcagcagtgg ctggtaaggc
     4441 tgatccagac cattaccccg gcttcgttcg aagcgtcaag gacgctcacc gctgcagagt
     4501 tgcccgaggc gatcgccgag gacggggcgg actggtgtcc ggcgcacatc ctgcattgcc
     4561 cgctgcgcgg gccgaacgac gccgcgcttg gcggaattct cttcttccgc gcacagccgt
     4621 tcaccgaggc ggagcacgcc gcggcggagt ggatagcgcg ctcaacgggc tatgccctgt
     4681 gggcctggcg cagcgagcgc agcggcatca agcgctggct gaagagccgc aagacctggc
     4741 gccagctcgc ggtcgccgcc gggctggccg cgcttgcttg cctcataccg gtgcggttga
     4801 gtgtcctcgc gccggcggaa atcacttccg ccaggccgat cccggtgacc tcgccgctcg
     4861 acggcgcggt gcgcgatatt ctggtcaagc cgaaccagat cgtgaaggcg gacgagctgc
     4921 tcgtggtgct ggacgatacc gggctcagga accgcctcga gctggcgtcc agagcgctcg
     4981 acatcgcgcg ggccgacctg cagcgcgcga cgttcaagtc gttcagcgac gaggccagcc
     5041 ggttcgagct gcaggtgctc aatgcccgca cgcaggagaa gctggccgag gtcacctacc
     5101 tgaccgagct gctggagaag tcgaagctca ccgcgccgca ggggggaatc gcggtcttct
     5161 ccagccagga ggattggcgc ggacgcccgg tgcaggtggg cgagcgcatc atgatgatcg
     5221 ccgacccatc cctcatcgac gtcacgatct acgttccgcc tgaagacgcc gtggagcttg
     5281 agcggggctc cgaggtcaac ctgctgctgc atgtggatcc gctgtcgccg ctggacgcaa
     5341 ggatcgagcg gtcgagctac gaggctaccg tcacgccgga cggcacgctc gcctacgtag
     5401 tgcgcgccca gcttctgccc ggccaggggc tgccgcgcat cggcctgcgg gggaccgcca
     5461 agatctatgc agatcgcgtg acgcttggat attacttgct gcgcaaaccg ctcgcctttt
     5521 tgcggcggag tctcggtatc tgagctctgt cggctcgggc gtgacgcaca atgctgccgt
     5581 gcgtcgatcc ggaggccgga atgcgagacc agcctttacg cttaggctcg ggcgacgcta
     5641 gattcgctcc tccatgactc ctttcgagct caccgaaccg aaatccctgc gcgaggcgat
     5701 cgaactgctc gatccggacg acaccgcagt gcgtcccatg tcgggaggca ccgctctcat
     5761 gctgatgatg aaagctggcg ttctacgccc gaggcgcctg gtcaatctga agaaaatagg
     5821 tcttgcagaa atcggcaaag ggccggccgg cgagcttcgc atcggcgcga tggcgcggct
     5881 ctcggacatc gagcgttcgc ccatcgtccg caaagattgt cctgtaattg cgcgcaccct
     5941 gctgactctg tcgaacgtgc gcgttcgcaa tgtcgccacc ctcggcggcc atctcgcgca
     6001 cgccgatccg cacatggacc tgccgccggt gctgagcgcg ctcggcgcgc gcgcgacgat
     6061 tgccgggccc gccggcgagc gcaccattcc ggtcgaagaa atcagctccg ggtacctggc
     6121 aaccacgctg cggttgaacg agctcatcac ggcgatcgaa gttccgccgc tcggcgggcg
     6181 tcgcgcggct tacatgaagt gcacgacgcg ttcggccgac gactggcccg ccctcggcgt
     6241 cgcagtcgtt ttggactttc aaaaggacgt attgcaaacg gcgagccttg tgatcagcgc
     6301 cgccaccgac aagccgacgc gattgcggtc tgcagagaag ctcctgcggg gaaagaccct
     6361 ggacgaaagc agcctcaagc aggccgggga agccgcagcc gcggaagtgc aaatggaaag
     6421 cgatatgcat ggctccgcgg cgtacaagaa gcagctgctg cgggtttacc tcgggcgagc
     6481 gattcatgaa gcgagcagcg ggcgccactg agacgccgca ggtcggccgc tcggtgccgc
     6541 ggcttgagtc gtggctgaaa gtcaccgggc gcgccgaata cgtccacaac ctgcgcctgc
     6601 ccggcatgct ctacggcaag attttccgct cgagcgtggc gcatggccgg atcaagcgca
     6661 tcgatgtgag cgtggcgcag gcggtgggcg gcgtgcatcg cgtggtgatc gcggcagact
     6721 tgaaggaaat cgtcccggat cctttttacg gcccggcgtt ccacgaccag ccgatcctcg
     6781 cggtcgacaa ggtgcgctac gtcggcgagc cggtggcggt ggtgctcgcc gccgaccccc
     6841 acgtggccga ggaggcggtc cacctcatca ctgccgagta cgaagagctg ccggcggtgt
     6901 acgacgaggt ggaggccatg acctcgaagg ccatcgtgca cgatgcactc aagcccgcag
     6961 gtacatttcc ggacctgaaa cacctgaaag gaagaaccaa caccaatatc gcgctcgact
     7021 ttcacctgcg ccgcggcgac gcggcgcgcg cgctgggcga cgcgcaccgc gttttcgagc
     7081 atcgattccg cacccaacag gtgatgcaca cgccgctcga gccgctggtc tcggtggccg
     7141 agtcgaccga gaactccctc accatccaca ccgcgtcgca gagcccgtcg ttcgtgcgca
     7201 tcgagattgc ccggcttctc ggctggccgg agaacaaggt cagggtcagg gtgccgttcc
     7261 tcggcggcgg cttcggcgcc aagctctaca tcaagctcga ggcgctcgtc agcgtgcttt
     7321 ctttactgac aaaaaaaccc gtcaaagttt cgctgacgat ggaggagcag ttcttcacca
     7381 tcaccaagca cgccagcacc ttccgcatca agagcggcgt gacgcgcgac gggcgcatcg
     7441 tcgcgcgcga gtgcgaggtg tggtggaacg gcggtgcgta tgccgatatc gggccgcgcg
     7501 tgacgcaaaa gtcgggcttc acggctcccg ggccctacga catcgagcac gtggcgatcg
     7561 actcctacgc gctctacacc aacctgccgc ccgccggcgc cttgcgaggc ttcggcatcc
     7621 cgcagctggt atgggcttac gagagccaca ccgacatgat ggcgcgcgag ctgggaatcg
     7681 atccgctcga attccgccgc atgaacctgc tgcgcgacgg ccgtccgcag gcgaccggca
     7741 ccgtgatgcg cgacgcggcc atcgaaaagg ttctcgatcg ccttgaatcc ctgatggatt
     7801 gggaaacacc atgggataaa ggcgaaggca gcgtgcgccg cgggcgcggc atcgcgatcg
     7861 gcttcaaggc ctgcgtgtcg cccaccagct cgctcgccgc ggtgagcgtg aatgccgacg
     7921 gcggctgtac cgtgtattgc agcacggtgg acatggggca ggggtccgat accgccatgg
     7981 cgcagatcgc gggcgaggcg ctgcacctgc cggccgagtc ggtgacggtg atgcattcgg
     8041 acaccgatgt caccccctac gacatggcga cgctcggctc gcgctcgctg ttccacatgg
     8101 gcaatgcggt caagctggcg gcggaggaag ccgggcgaaa actgcaggcg ttgagagaag
     8161 aactgaaact tcccgcggat gcggaaatcc cggacatttt cagaaagaag tacggcatgc
     8221 aggcgggcag cgtgctcggc accggcagct tcattccaag ctacgccgcg cccgaccatg
     8281 ccaccggtca gacgccgaac gcgacgccct tctggatggt gggcggcacg ggcgtggagc
     8341 tcgaggtcga taccgagacc ggccacgtgc gcatcgcgcg cctggtcaac gtcgccgacg
     8401 tcggcacgcc gattaacccg cgcatcgtcg agacgcagct ctcgggcgcc gcgatcatgc
     8461 agctcggctt cacgctgttc gagaaaatgg tgttcgacgc cgacgggcag ttgcgcaacg
     8521 cctcgctcgc cgagtacaag attcccggcc tgctcgacct gccgcgggaa atcatcaatg
     8581 aagccgtgac cgcggcgcag cgttccgggc cattcggcgc gaagggcgtc ggcgagaccg
     8641 gaaccttcgg cgtgtcgccc gccatcgcca acgcgataca cgatgcggtc ggcgtcaggg
     8701 tgacttccct gccgctcaat ccggaaacca tcttcatcgg acttcaaaaa ttgagatgag
     8761 acgcgcgatt caattccggc tgaatggcga ggctgtcgag gcgcaggtcg cgccgcacga
     8821 gagcctggtg gaggtgctgc gcgacgcgtt cggcctatac ggcgcgcgcg agagctgcgg
     8881 ccagggcctg tgcggctgct gcactgtgct ggtcgatggg cgcgcggtct cgggctgcct
     8941 gtacctggcc tcgttcgccg cgggcgcgga agtgagcacg gtggaggccg gcaaggagct
     9001 cgatgcggtg caggaagcct tcacggagcg agccgccttc cagtgcggct tctgcacccc
     9061 cggcttcatc ctcatgacgc gccagctgct ggacgagaat cccgacccgg acgacgacgc
     9121 gatccggcat tacctgtcgg gaaacctgtg ccgctgcgcg gcctatcccg agatcatcga
     9181 agcagttaag ctcgctgcaa ggaaacggag gaagtgatga agctcttgct cgccgctctg
     9241 gcgcttgctg cgtcgctggc ccaggcccag gactatccca acaagccgat ccacatgctg
     9301 gtgccgttcg cgccgggcgg cgcggtcgac acgacagcgc gcgtgctggc gcaggccatg
     9361 cagcagcgcg tgaaatggca gttcgtgatc gagaaccgcc ccggcggcaa cggcttcatc
     9421 gccaccacgg cggtcgcgaa gagcccggcc gacggttaca cgctgctgat ggcgcacacc
     9481 ggcgagttcg ccgtgaaccc ggcgctcttt gacaacatcc cctacgagct cgagcgcgac
     9541 ttcgttccca tcaccatggt aagtgactcg ccgatgcttt atctcgccaa cgcgcaggcg
     9601 ccgttcaata ccttccagga actcgtcgcg caggcaaagg ccaagcccgg cgccatcagc
     9661 ttcggctcgg cgggcaacgg cagcatcaac cacctcgctg gcgagtggct cgcgcaggcg
     9721 gcggggatcc agctcctgca cgtaccttac aagggcggcg cgccggccgc cgcggcggtg
     9781 gccgcgggcg acgtgcactt tggcgtgatg gcggtgccgg gagcgatgcc ccacatcaag
     9841 tcgggacgcg gcaaggtcat cggcatcacc acggcgaaga agtctccgct cgatccgtcg
     9901 tgggccacgc cgcgcgacgc cggcatcccg gacctcgatg cctcgatctg ggtagggctg
     9961 ttcgcgccga agggcacgcc gcaagccgtc gtcgaccgca tcaacaagga agtgcgccag
    10021 acgctggcca tgccggacgt gcagaaacgc ttcatcgaga gcgccggcgg cgaggcggtc
    10081 ggcatgacgc cggccgagtt cctggcgcgc atcaagagcg acgccgagcg ctacaagcgc
    10141 gtggtgcaga aggccggcgt caagcccggc tgatccaatg gaggagctgg tcgagcctct
    10201 ttggcagcgt gccgacgaag gcgcggaatg cctggggctc gagccattcc catacgccca
    10261 ggccgatgat cgcgatcccg gccgcggcga ccaggagacc cagcagcgct cgcggaaagt
    10321 acactaggat cctccagatc cagctgcttc gcccctgggg gttgagcatc atcaggacgc
    10381 cgatgccgat gacgaacaat cctcccgtgc cgaggagagg ggcggggcgg cgcatcaggt
    10441 agttcaccgt cgactgccac tggtcgttgt cgagaaggta ggccgccgcg atcatccccg
    10501 cgccgacgac cagcaccatc atgccgacga tcagcgccgg cgcgcccgcg tagttcgcat
    10561 acacgtcgtc ggagggacgg aaacacatgc ggcgggtggc gatggcatcg ataccggcga
    10621 gcgcgaagcc cgcgccggcc acgaacacgc caagatgaaa gcccttggcg agtccgaagt
    10681 actggccggc gaccatcccg acgctgccga tgatcactgc gacatattcg aggaacgcga
    10741 tcagccgcat ttggccggca gtttaaccgg ctcacagcga gaccgccgcg ctgcattacc
    10801 tagctttcga agaattcctt gaccttgtcg aaccagccct tggcgcgggg gttgtggcgc
    10861 gcgctgtcct gctgggtgat ggcgtcgaac tcgcgcagca actggcgctg gcgctcggtc
    10921 aggctgaccg gggtctcgac caccacgtgg cagaacagat cgccgtgggc ctggctgcgc
    10981 acgcctttta tgcccttgcc cttcaggcgg aaggtcttgc cggtctgcgt ctcggccggg
    11041 acgcggatct tcgcagagcc gtcgagcgtc gggatcgaga tctcgccgcc gagcgcggca
    11101 gcagtgatcg agatcggcat ctcgcagtgc aggtcgtcgt ggtcgcgcgt gaacaccgga
    11161 tgcggcttga tgtgcacctg gacgtagaga tccccgggcg ggccgccgtt gacgccgggc
    11221 tcgccttcgc ctgacaggcg cacgcggtct ccctggtcga cgcccgcggg gatcttcacc
    11281 gacagcgtct tctgcagctt cacgcgcccc gatcccgagc attgcgcgca ggggttcgga
    11341 atcacgctgc cgctgccgtg gcagcgcggg caggtctggg cgatcgagaa cgggccctgg
    11401 gatacgcgca cctggccggc gccgcggcac gtgggacagg tggcgggctg ggtgccgggc
    11461 cgggcgcccg tgcccttgca gatatcgcat gcggccatcg ccgggatacg gatcttggtc
    11521 tcgaagccgt gcgccgcctg ctcgagcgtg atctccaggt tgtagcgcag gtcggcgccg
    11581 cggaacacgg tcgagcggcc gctgcggccg ccgccgaaga tctcgccgaa gatgtcgctg
    11641 aagatgtcgc cgaagccgcc catgctgccg gccgccgcgc ccgcagcgtg cggatcgacg
    11701 ccggcgtggc cgaactggtc gtaggctgcg cgcttgcccg cgtcggagag gatctcgtag
    11761 gcttccttcg cttccttgaa gcgctcctcg gccttgggat tctccggatt gcggtccggg
    11821 tgccacttca tcgccagttt gcgataggcg ttcttcaggt cgccgtcggc ggcgtcccgg
    11881 ttgactccga gcacctcgta ataatcgcgc ttcgccatgc ccgaagcctt cctcccgaaa
    11941 ggagaagctc agccggtctt cttgtccttc acctcggtga actcggcatc gacgacgttc
    12001 tcgtccttct tcgccggctc ggcctgcgcc tgctg
//
LOCUS       DASPXW010000017         2930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_5775, whole genome shotgun sequence.
ACCESSION   DASPXW010000017 DASPXW010000000
VERSION     DASPXW010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5775"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2874
                     /locus_tag="VEL04_00385"
     CDS             <1..2874
                     /locus_tag="VEL04_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013204980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HYT46851.1"
                     /translation="FFEMEKIRHIERYTGGKFKALELDITYPAAWGKEAIEARLASLC
                     AQAEDAVRSGYSIVIVSDRLVNRERVAIPALLALSAIHQHLVNKGLRTSTGLVVETGS
                     AREVHHFALLGGYGAEAVHPYLALETLLAMHPEDRAAGSKAIKHYVKAVGKGLKKVMS
                     KMGISTYMSYTGSQIFEALGLAHSLVDKYFTGTTSTVEGIGVFEVADEAIRIHQAAFG
                     DRDPVLATMLDAGGEYAWRVRGEDHTWTPDAIAKLQHAARQNSYSTYKEYAALINDQA
                     KRHLTFRGLFDFKFGSRAPLPIEEVEPAAEIVKRFSTGAMSLGSISTEAHTVLAIAMN
                     RLGGRSNTGEGGEDRKRYAPVKEGESLMSRLGRERVEVDVPLRAGDSLNSKIKQVASG
                     RFGVTAEYLANAQMLQIKIAQGAKPGEGGQLPGRKVSEYIAELRYSTPGVELISPPPH
                     HDIYSIEDIAQLIHDLKNASPDAWVSVKLVSEVGVGTIAAGVSKAKSDHVTIAGHDGG
                     TGASPWSSIMHAGTPWELGLAETQQTLVLNRLRGRIAVQVDGQMKTGRDVVIGAILGA
                     DEFGFATAPLVVEGCIMMRKCHLNTCPVGVATQDPELRKKFEGKPEHVVNYLFFVAEE
                     ARELMAQLGIRKFNDLIGRADLLDTRKGIEHWKAKGLDFSKVFYTPQMPPEVARYKCE
                     AQNHALEKALDNRLIELARPALERGEKVTIETPIRNINRTVGTMLSNRIARRYGHEGL
                     PDETIHVRLAGSAGQSFGAFLARGVWLDLIGDTNDYCGKGLSGGRISVQPSPKFRGEP
                     TENIITGNVVLYGAIAGEAYFRGVAGERFAVRNSGARAVVEGVGDHGCEYMTGGAVVV
                     LGVTGRNFAAGMSGGFAYVLDVDGEFARRCNTAMVDLEPLVESDDEAAVKRLIENHAR
                     YTNSRRAAEILEKWAQYRPRFVKVFPKEYRRALAELSQKKVAA"
BASE COUNT          536 a          965 c          975 g          454 t
ORIGIN      
        1 ttcttcgaga tggagaagat ccgccacatc gagcgctata ccggcggcaa gttcaaggcc
       61 ctcgaactcg acatcaccta cccggcggcc tggggcaagg aggcgatcga ggcgcggctc
      121 gcctcgctct gcgcgcaggc cgaggacgcg gtgcgctcgg gctattcgat cgtcatcgtc
      181 tccgaccgct tggtaaatcg cgagcgggtc gcaatcccgg cgctgctcgc gctctcggcg
      241 atccaccagc acctggtcaa caagggcctg aggacctcca ccgggctcgt ggtcgagaca
      301 ggttcggcgc gcgaggtgca ccactttgcc ctgctcggcg gctacggagc ggaggccgtg
      361 cacccgtacc tcgcgctcga aacgctgctt gcgatgcacc ccgaggatcg cgcggcgggt
      421 tccaaggcga tcaagcacta cgtcaaggca gtcggcaagg gcctgaagaa ggtgatgtcc
      481 aagatgggca tctcgaccta catgtcgtat accggttcgc agatcttcga ggcgctgggg
      541 ctcgcgcact cgctcgtcga caagtacttc accggcacca cctcgacggt cgagggcatc
      601 ggcgtgttcg aggtggcgga cgaagcgatc cgcatccacc aggccgcgtt cggcgacaga
      661 gacccggtgc tcgcgacgat gctggacgcc ggcggggaat acgcctggcg cgtgcgcggc
      721 gaggatcaca cctggacgcc cgacgccatc gccaagctgc agcatgcggc gcggcagaac
      781 tcttactcga cctacaagga gtacgccgcg ctcatcaacg accaggcgaa gcgccatctg
      841 accttcagag gcttgttcga cttcaaattc ggcagccgcg cgccgctgcc gatcgaggag
      901 gtcgagcccg ccgccgagat cgtgaagcgc ttctcgaccg gcgccatgtc gctcggctcg
      961 atctcgaccg aggcgcacac ggtgctcgcc atcgcgatga accgcctcgg cggcagatcg
     1021 aataccggcg agggcggcga ggaccgcaag cgctatgcgc cggtgaagga gggtgaaagc
     1081 ctgatgtcgc ggctcggccg cgagcgcgtc gaggtggacg tcccgctccg cgccggcgat
     1141 tcgctcaatt ccaagatcaa gcaggtcgct tcgggccgct tcggcgtcac cgccgaatac
     1201 ctcgccaacg cgcagatgct gcagatcaag atcgcgcaag gcgccaagcc cggcgagggc
     1261 gggcaactcc cgggccgcaa ggtctccgag tacatcgccg agctgcgtta ctcgactccc
     1321 ggggtcgagc tcatttcacc gccgccgcac cacgacatct actcgatcga ggacatcgca
     1381 cagctcatcc atgacctcaa gaacgccagc cccgacgcct gggtgagcgt gaagctcgtg
     1441 tccgaggtcg gcgtgggcac gatcgcggcg ggcgtctcca aggcgaagtc cgatcacgtc
     1501 accatcgccg gccacgacgg cggcaccggc gcctcgccct ggtcctcgat catgcacgcc
     1561 ggaactccct gggagctcgg gctcgccgag acccagcaga cgctggtgct caaccgcctg
     1621 cgtggccgca tcgcggtgca ggtggacggc cagatgaaga ccggccgtga cgtagtgatc
     1681 ggcgcgatcc tcggcgcgga cgaattcggt ttcgccaccg ctccgctggt ggtcgagggc
     1741 tgcatcatga tgcgcaagtg ccacctgaac acctgcccgg tcggcgtggc gacccaggac
     1801 ccggaactga ggaagaagtt cgagggcaag cccgagcatg tcgtgaacta cctgtttttc
     1861 gtcgccgagg aggcgcgcga gctgatggcg cagctcggta tccgcaagtt caacgacctg
     1921 atcggccgcg ccgatttgct cgacacgcgc aaaggtatcg aacactggaa ggcgaaaggg
     1981 ctcgacttct cgaaggtttt ctacacgccg cagatgccgc ccgaagtcgc gcgctacaag
     2041 tgcgaggcgc agaaccacgc cctcgagaag gcgctcgaca accgcctcat cgagctggcg
     2101 cgcccggcgc tcgagcgcgg cgagaaagtg accatcgaga cgccgatccg caatatcaac
     2161 cgcaccgtcg gcaccatgct ttcgaaccgg atcgccaggc gctacggcca cgaagggctg
     2221 ccggacgaga cgatccacgt gcggcttgcc ggctcggcag gtcagagctt tggtgcgttc
     2281 ctcgcgcgcg gcgtgtggct cgacctgata ggcgacacca acgactactg cggcaagggg
     2341 ctctcgggcg gcaggatctc ggtgcagccc tcacccaagt tccgcggcga gcctacggag
     2401 aacatcatca ccggcaacgt cgtgctgtac ggcgccatcg ctggcgaggc gtatttccgc
     2461 ggcgtggccg gcgagcgctt tgccgtgcgc aactccggcg cccgcgcggt ggtcgaaggc
     2521 gtgggcgacc acggctgcga atacatgacc ggcggtgcgg tcgtcgtgct gggcgtcacc
     2581 ggccgcaatt tcgccgccgg catgtcgggc ggcttcgcct acgtgctcga cgtcgatggc
     2641 gagtttgcca ggcgctgcaa cacggcgatg gtggacctcg aacccttggt tgaatccgac
     2701 gacgaggcgg cggtgaagcg cctcatcgag aatcatgccc ggtacaccaa ttcgaggcgc
     2761 gccgccgaaa tcctcgaaaa atgggcgcaa taccggccgc gcttcgtcaa ggttttcccg
     2821 aaggagtacc ggcgcgcgct ggccgagctg tcgcagaaga aggtggccgc ctaaggggga
     2881 aatgagtggg taaggtcacc gggtttctcg agtacgggcg catcgaggag
//
LOCUS       DASPXW010000018         5680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1393611, whole genome shotgun sequence.
ACCESSION   DASPXW010000018 DASPXW010000000
VERSION     DASPXW010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5680
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1393611"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VEL04_00390"
     CDS             complement(<1..183)
                     /locus_tag="VEL04_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT46852.1"
                     /translation="MAEPLAGPEEDGLLVDGTFGRRIRTLARSGPLHQLVRNAAYQDG
                     DWSCYDLLALELAAIDY"
     gene            complement(421..2136)
                     /locus_tag="VEL04_00395"
     CDS             complement(421..2136)
                     /locus_tag="VEL04_00395"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HYT46853.1"
                     /translation="MKKYEHLLYSAIGLAALLLLLVAANYLISRVPGRLADLTDGRLY
                     TLSDGTKKILRGLASPVRLKLYISQGETVPVPLRSFSQRVEDLVREFKAVAGSNLSVE
                     RYNPKPDSEEEDAAQLDGIEPQQLYSGEQFYLGAAVSQLDRKQAIAAISPQRERLLEY
                     DLIRAIARVAQPERPRVGLMAGLPVLGERFNPFTRQSSDPWVLANELKREFEVKEVPM
                     SAKEIGKDIGVLLVIHPRDIQPETEYALDQFVLRGGKLIAFVDPYAYFDQTPTMPGMP
                     PQQGSSSTLPTLFKAWGVQMDPGKVVSDVVFGSGSGARYTPTVLSLNRTAFSRDDIVT
                     GTIETLLYAFGGALEINNSELKITQLVRSSPNSMLVDTANATKSGDEATRSFKPTGKP
                     LPLAVRLTGKFRTAFPEGLSIDKKPLPGTPQLRESSGENALILVADVDMLADGAAVDV
                     QDAFGRRVVVPSNGNLALALGMVELAGRAGGRLDAVFLDEGFGGLDTSSLDAAIDALE
                     TRAASGRLVAVISHVQLVAERITDVLGVRATPAGSEVDWLSTSERAEAVDQELAATIE
                     AGLLG"
     gene            complement(2141..2875)
                     /locus_tag="VEL04_00400"
     CDS             complement(2141..2875)
                     /locus_tag="VEL04_00400"
                     /inference="COORDINATES: protein motif:HMM:NF024141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYT46854.1"
                     /translation="MRIIWTIAKRELAAYFNSPVAYVFLVIFLLLTGFFTFTAGNFFE
                     RGEASLAAFFGWHPWLYLVLVPAVGMRVWAEERRSGTMELLLTMPVATWQAIVAKFLA
                     PWLFLAFALALTFPAIITVNVLGEPDNGVIVAGYLGSLLLAGAYLAITCMTSAMTRNQ
                     VVAFILSVGLCLFLILAGFNPVTDLLARWASPALVDTVAAFSVVTHFDGFQRGVIDAR
                     DLAFFLSLIGFALFATGVVIRGHRSG"
     gene            complement(2872..3807)
                     /locus_tag="VEL04_00405"
     CDS             complement(2872..3807)
                     /locus_tag="VEL04_00405"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYT46855.1"
                     /translation="MIKIENLVKAFGAKRAVDDISFSVERGEVLGFLGPNGAGKSTTM
                     RMITGFMPPTSGKISIGAHDVAESPLEAKRLIGYLPENAASYPDMTVQGFLNFAAELR
                     GLQGAARRKAVHRVVELCFLDSVLQQSIDTLSKGYKHRTCLAQALIHDPQVLVMDEPT
                     DGLDPNQKHEVRNLIRELGANKAIVFSTHILEEVDAACTRAIIIDRGKLVANGTPEEL
                     RGMSQLAGAVTLQARGASLEALSRIGKVEELDGAFRIYPADKSKSKQLAQAVIELVAR
                     EGWKVEGMYNEAGQLDEVFRRITLPDTVKQDTVKK"
     gene            3887..4846
                     /locus_tag="VEL04_00410"
     CDS             3887..4846
                     /locus_tag="VEL04_00410"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYT46856.1"
                     /translation="MRALFGSVFATLLAFSTAAQAQQYPVKPVRIVVPFAPGGGSDFI
                     ARFIAQRLGAGLGQQAIVENKPGAGGMLGIEAGVKSPPDGYTLTLIASSYTVNPSIYK
                     FSFDPVSDITPIIQLSQGPLLVVVRPSLPVKSTQELIALAKAKPGQINFASSGQGSVI
                     HMATELFQSMAGIKMNHIPYKGTGPALTDTIGGQTDVFFSSTATAVPHVQSGKLRAIA
                     VTSAKRIAALPNVLTMAESGLPGYEVTLWHGLIGPKGLPRPIVERLNGEATKALKLKE
                     TAAQLQNDGVAPAGGTPEQFLAQIKREIGVWRKVASDAGVKAE"
     gene            4863..>5680
                     /locus_tag="VEL04_00415"
     CDS             4863..>5680
                     /locus_tag="VEL04_00415"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYT46857.1"
                     /translation="MVVGAGSAGLAAARELAEAGRSVLVLEARDRVGGRCWTRRMPGL
                     EIPVELGAEFIHGGPEITLALLREAGIPAVESTREQRYLEGGRLRSIDAFAHARQAVQ
                     QVSVLERDVSFQTFLARRRLPQKTRSFARMMVQGFDAADPKRVSARSIAQEWGEAGAL
                     GASQPRPLGGYGALLDWLAHSAVKKGACLQLESTVRELRWKRGAVIVRGSFLGQAFVA
                     KAARAIVALPLGVLQSGAVHFAPPLQEKRPALKKLAVGPVIRVALCFQSAFWER"
BASE COUNT          950 a         1896 c         1909 g          925 t
ORIGIN      
        1 gtagtcgatc gccgccagct ccagcgccag caggtcatag caggaccagt cgccgtcctg
       61 gtacgcagcg ttgcggacaa gctgatgcag cgggccgcta cgcgccagcg tccggatccg
      121 ccggccaaac gtcccatcga ccagcagccc gtcctcctcc ggccctgcca acggctccgc
      181 cattccccgc accggctcct accacgttgg gtttgcctgc ttggctgcaa ccccgagaga
      241 acattcacgc ctgccgaccc gtcactggcc gcggcgtatc ccaaacctca gcgttgccgc
      301 aactatagac gtttgttccg acagtcttgg ccgatgcatt tgcggttctt cagcccgaca
      361 ccccgccccg caaagcgcac ggggtgaaat cgaacccggc acctggccac gagcctgcgg
      421 ctagccgagc aggccggctt cgatggtggc ggcgagttcc tggtcgaccg cctcggcccg
      481 ctcggaggtt gagagccagt cgacctcgct gccggccggc gtggcccgca cgccgagcac
      541 gtcggtgatc cgctcggcca ccagctggac gtggctgatg accgccacca gccggccgct
      601 ggcggcgcgg gtctccagcg cgtcgatggc ggcgtccagg ctactggtat ccagcccgcc
      661 gaagccctcg tcgaggaaca ccgcgtccag gcggccgccg gcccggccgg cgagctcgac
      721 catgccgagc gcaagcgcga gattgccgtt cgagggcacc accacccggc ggccgaacgc
      781 gtcctgcacg tcgaccgcgg cgccgtcggc gagcatgtcg acgtctgcga ccaggatcag
      841 cgcgttttcg cccgacgatt cgcgcagctg cggcgtcccc gggagcggtt ttttgtcgat
      901 cgataaaccc tctggaaacg cagtcctgaa cttgcccgtt aggcgcacgg cgagcggcag
      961 cggctttccg gtgggcttga agcttcgggt cgcctcgtcg ccggacttgg tggcgttcgc
     1021 ggtgtccacg agcatcgagt tcggcgagct tcttaccagc tgcgtgatct tcaactcgga
     1081 gttgttgatt tcgagcgcgc cgccaaacgc gtagagcaga gtctcgatgg tgccggtgac
     1141 gatgtcatcg cggctgaagg ccgtacggtt gagcgagagc acggtgggcg tgtagcgcgc
     1201 gcccgagcct gaaccgaaca ccacgtcgga caccaccttg ccggggtcca tctgcacgcc
     1261 ccaggccttg aacagcgtcg gcaaggtcga actgctgccc tgctgcggcg gcatgcccgg
     1321 catggtcggc gtctggtcga aataggcgta gggatcgacg aacgcgatga gtttcccacc
     1381 gcgcagcacg aactggtcga gcgcgtactc ggtctcgggc tggatgtcgc gcgggtggat
     1441 caccagcagc acgccgatgt ccttgccgat ctccttggcg ctcatcggca cttccttcac
     1501 ctcgaactcg cgcttgagct cgttggcgag cacccaggga tcggagctct ggcgcgtgaa
     1561 cgggttgaag cgctcgccga gcaccggcag cccggccatg agtccgacgc gcggccgctc
     1621 gggctgcgcg acgcgcgcga tggcgcggat caggtcgtat tccagcagcc gctcgcgctg
     1681 cggggagatc gcggcgatcg cctgtttgcg gtcgagctgg cttaccgccg cgccgagata
     1741 gaactgctcg cccgagtaga gctgctgcgg ctcgatgccg tcgagctgcg cggcgtcctc
     1801 ttcctccgag tcgggcttgg ggttatagcg ctcgacgctc aggttggagc ccgccaccgc
     1861 cttgaactcg cgcaccaggt cctcgacgcg ctgcgagaag ctgcgcagcg gcacgggcac
     1921 cgtctcgccc tgcgagatgt agagcttgag acgcaccggc gaagcgagcc cgcgcaggat
     1981 cttcttggtg ccgtcggaga gcgtgtagag cctgccgtcg gtgaggtcgg cgaggcggcc
     2041 ggggacgcgc gagatgaggt agtttgccgc caccagcagc agcaacagcg ccgcgaggcc
     2101 gatcgccgaa tagagcaggt gctcgtattt cttcatgctc tcagcccgag cggtggccgc
     2161 ggatcaccac tccggtcgcg aacaacgcga agccgatcag cgacaggaag aacgccagat
     2221 cgcgcgcgtc gatgacgccg cgctggaagc cgtcgaaatg cgtcaccacc gagaacgccg
     2281 ccaccgtgtc gaccagcgcg gggctcgccc agcgcgcgag cagatcggtc accgggttga
     2341 agccggcgag gatcaggaac aggcacaacc ccaccgaaag gatgaaggcg accacctggt
     2401 tgcgtgtcat cgccgaagtc atgcaggtga tggcgagata ggcgccggcg agcagcaggc
     2461 tgccaaggta gcccgcgacg atgacgccgt tgtccggctc gcccagcacg ttgaccgtga
     2521 tgatcgcggg aaaagtaagc gcgagcgcga aggcgaggaa cagccagggg gcgaggaact
     2581 tggcgacgat cgcctgccag gtcgcgaccg gcatggtgag caacagctcc atcgtccccg
     2641 agcgccgctc ttcggcccag acgcgcatgc cgacagccgg cacgaggacc aggtacagcc
     2701 agggatgcca gccgaagaac gccgccaggc tcgcctcgcc gcgctcgaag aaattgcccg
     2761 cggtgaaggt gaagaagccg gtgagcagca ggaagatcac caggaacacg tacgccaccg
     2821 gggagttgaa ataggccgcg agctcgcgct tggcgatggt ccagatgatc ctcatttctt
     2881 caccgtgtcc tgcttcacgg tgtccggcag cgtgatgcgg cggaacacct cgtcgagctg
     2941 cccggcctcg ttgtacatgc cctcgacctt ccagccttcg cgcgccacca gctcgatcac
     3001 cgcctgtgca agctgcttcg atttcgattt gtcggccgga tagatgcgga atgcgccgtc
     3061 cagttcttca actttgccta ttctggataa tgcttcgagc gaggcgcccc gcgcctggag
     3121 cgtcaccgcg ccggcgagct gcgacatgcc gcgcagctcc tcgggggtgc cgttggcgac
     3181 gagcttgccg cgatcgatga tgatggcgcg cgtgcaggcg gcgtcgacct cctccaggat
     3241 gtgggtggag aacacgatcg ccttgttcgc gcccagctcg cggatcaggt tgcgcacctc
     3301 gtgcttctgg ttgggatcga ggccgtcggt cggctcgtcc atgaccagca cctgcgggtc
     3361 gtggatcagc gcctgcgcga ggcaggtgcg gtgcttgtag cccttggaga gcgtgtcgat
     3421 cgactgctgc agcaccgagt cgaggaagca cagctcgacc acgcgatgca ccgccttcct
     3481 gcgcgccgcg ccctgcaggc cgcgcagctc ggcggcgaaa ttcagaaagc cctgcaccgt
     3541 catgtcggga tacgaggcgg cgttctcggg caggtagccg atcaggcgct tcgcctcgag
     3601 cggcgactcg gcgacgtcat gggcgccgat cgagatcttt cccgaggtcg gcggcatgaa
     3661 gccggtgatc atgcgcatgg tggtcgactt gccggcgccg ttcgggccga ggaagccgag
     3721 cacctcgccg cgctcgacgc tgaacgagat gtcgtccacg gcgcgcttgg caccaaacgc
     3781 cttcaccaga ttttcgatct tgatcatgga ctagaattgc gaagggcgcg attatcgcgc
     3841 aagttcaggc caagtccaca tcgatgttcc cccgaggaaa gagatcatgc gcgcgctatt
     3901 tggttccgtg ttcgcgactc tgcttgcctt ttcgactgcg gcccaggcgc agcagtatcc
     3961 ggtcaagccg gtgcggatcg tcgttccctt cgcgccgggc ggcggctccg atttcatcgc
     4021 ccgattcatc gcgcagcggc ttggcgcggg cctcgggcag caggcgatcg tcgagaacaa
     4081 gcccggcgct ggcggcatgc tcggcatcga ggccggagtc aagtcgccgc cggacggcta
     4141 cacgcttacc ctgatcgcct ccagctacac cgtgaatccg agcatctaca agttcagctt
     4201 cgacccggtg agcgacatca cgccgatcat ccagctgtcc caggggccgc tgctcgtcgt
     4261 ggttaggccc tctctgcccg tgaaatcgac gcaggagctg atcgcgctgg cgaaggcgaa
     4321 gccgggccag atcaacttcg cctcgtcggg gcaggggagc gtgattcaca tggcgacaga
     4381 gctgttccaa agcatggcgg gaatcaagat gaaccacatt ccctacaagg gaacgggccc
     4441 ggcgcttacc gacacgattg gaggacagac cgacgtcttc ttcagcagca ccgccacggc
     4501 ggtgccgcac gtgcagtcgg gcaagctgcg ggcgatcgca gtcacttccg cgaagcgcat
     4561 cgcggcgctc cccaacgtac tgaccatggc cgagtcgggt ttgcccggct acgaggtcac
     4621 gctgtggcac ggcctgatcg ggcccaaggg gctgccgcgc ccgatcgtgg agcggctcaa
     4681 cggcgaagcg acgaaggcgc tcaagctcaa ggagacggcc gcgcagctgc aaaacgacgg
     4741 cgtcgccccg gcgggaggca cgcccgagca gttcctcgcc cagatcaaga gagaaatcgg
     4801 cgtctggcgc aaggtcgcct ccgacgccgg cgtcaaggcg gaatagcacg gccttcgacg
     4861 taatcgtcgt cggcgccggc tccgccgggc tcgccgcggc gcgcgagctt gccgaggccg
     4921 ggcgctccgt gctggtgctc gaggcgcgcg atcgcgtcgg cgggcgctgc tggacgcgcc
     4981 gcatgccggg gctcgagatt cccgtcgagc tcggcgccga gttcatccac ggcgggcccg
     5041 aaatcacgct cgcgctgctt cgcgaggcgg gcattcccgc ggtcgaatcc acgcgcgagc
     5101 agcgctatct cgagggcgga cggctgcgtt ccatcgacgc cttcgcccac gcgcggcagg
     5161 cggtgcagca ggtatcggtg ctcgagaggg acgtatcgtt tcagaccttt cttgcccggc
     5221 gacgcctgcc gcagaaaacc cggagcttcg cgcgcatgat ggtccagggc ttcgacgccg
     5281 ccgatccgaa acgcgtgagc gcgcgctcga ttgcgcagga gtggggcgag gccggagcgc
     5341 tcggcgcctc gcagccgcgg ccgctcggcg gctacggcgc gctgctggac tggctcgcgc
     5401 attctgccgt caaaaaggga gcgtgcctgc agctcgaatc gaccgtccgc gagctacgtt
     5461 ggaaacgcgg tgccgtgatc gtgcgcggaa gcttcctcgg ccaagcattc gtcgccaaag
     5521 cggcgcgcgc catcgtcgcg ctgccgctag gggtactgca gtccggtgcg gtccatttcg
     5581 cgccgccgct gcaggaaaag cgccctgcgc tcaaaaagct ggccgtcgga cctgtgatcc
     5641 gcgttgcgct gtgcttccaa agcgccttct gggagcggca
//
LOCUS       DASPXW010000019         2438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1140805, whole genome shotgun sequence.
ACCESSION   DASPXW010000019 DASPXW010000000
VERSION     DASPXW010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140805"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2294
                     /locus_tag="VEL04_00420"
     CDS             <1..2294
                     /locus_tag="VEL04_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HYT46858.1"
                     /translation="LNMDSSLGSQFRERLEFLDFKDKLLARPRVTAIRQPYFCSGCPH
                     NTSTRVPEGSRATAGIGCHFMALWMDRSTATFTHMGAEGAPWIGQAPFTDEKHIFTNL
                     GDGTYFHSGLLAIRAAVAAKVNMTYKILYNDAVAMTGGQRHDGPLDPARISRQIAAEG
                     VSPIVVVTDEPQKYPAGVNWAPGVTVRHRDELDAVQRELRQMPGVSALIYDQTCASEK
                     RRRRKRNEYPDPAVRAVINERVCEGCGDCSEKSNCLSVEPLETEFGRKRVINQSSCNK
                     DYSCVNGFCPSFVTVEGGRLKKGKAGAVPTDEAFSLPEPENPSLQTPYGILVTGIGGT
                     GVITIGQILGVAAHLEGKGVTVLDMSGLAQKYGAVMSHVQVAARPEALHAARLDTGGA
                     SLVIGCDMVVTASNDAIAKMSAARTRAVVNASVTPTAEFVRNPNWQLPGSNLHDDIAQ
                     ACGAGSSVFIPATELASGLMGDAIATNMFMLGYAYQKSWIPVSAAALERAIELNGVAV
                     EFNRKSFTWGRRAAVDLERVRRIAVPAEVISIEQHFSRNLDEIVERREKFLIAYQDAA
                     YAARYRQLVEKVKTHEKEKLGSTKLAEAVARYYAKLLAYKDEYEVARLHAEGGFTKRI
                     RDMFEGDYHVVYHLAPPLLAGKDPLTGEPRKMRFGPWMLPVFKLLKNLKLLRGTSFDL
                     FGYTQERRTERALISEYEQTVERLLQGLSPRNHAVAVEIATLPEEIRGFGHIKMKNVV
                     AVAKKRDALLQRFGSPAAERAAA"
BASE COUNT          463 a          809 c          782 g          384 t
ORIGIN      
        1 aattgaacat ggattcttcc ctgggaagcc agtttcgcga gcggcttgag tttctcgact
       61 tcaaagacaa gctgctcgcc aggccgcgcg tcaccgcgat ccgtcagccc tacttctgct
      121 cgggctgtcc gcacaacacc tccacccgcg tgcccgaggg cagccgcgcc accgccggca
      181 tcggctgcca tttcatggcg ctgtggatgg atcgcagcac cgccaccttc acgcacatgg
      241 gggccgaggg cgcgccgtgg atcgggcagg cgccgttcac cgacgagaag cacatcttca
      301 ccaacctcgg ggacggcacc tacttccact ccggcctgct cgcgatccgc gccgctgtgg
      361 cggcgaaggt caacatgacc tacaagatcc tgtacaacga cgccgtggcg atgaccggcg
      421 ggcagcgcca tgacggcccg ctcgacccgg cgaggatctc gcgccagatc gcggcggaag
      481 gcgtgagccc catcgtcgtc gtcaccgacg agccgcaaaa atatcccgcc ggcgtgaact
      541 gggcgccggg cgtgaccgtg cgccatcgcg acgagctcga cgccgtgcag cgcgagctgc
      601 gccagatgcc gggcgtgtcg gcgctgatct acgaccagac ctgcgcttcc gagaagcgcc
      661 gtcggcgcaa gcgcaacgag taccccgacc ctgcggtgcg cgcggtgatc aacgagaggg
      721 tgtgcgaggg ctgcggcgac tgcagcgaga aatcgaactg cctgtcggtc gagccgctcg
      781 agaccgagtt cggcaggaag cgcgtcatca accagtcctc gtgcaacaag gactattcgt
      841 gcgtcaacgg cttttgcccg agcttcgtga cggttgaagg cggccgactg aagaagggca
      901 aggccggcgc ggttcctacc gatgaagctt tttctctccc ggaaccggaa aacccctccc
      961 ttcaaacccc ttatgggatt ctcgtcaccg gcatcggcgg caccggggtc atcaccatcg
     1021 gccagatcct cggcgtggcg gcgcacctcg agggcaaggg tgtcaccgtg ctcgacatgt
     1081 cgggcctagc gcagaaatac ggcgccgtga tgtcgcacgt gcaggtggcg gcgcggcccg
     1141 aggcgctgca tgcggcgcgg ctcgacaccg gcggcgcgag cctggtgatc ggctgcgaca
     1201 tggtcgtcac ggcaagcaac gatgcgatcg cgaaaatgtc ggctgcgcgc acgcgcgccg
     1261 tggtcaatgc aagcgtcacg ccgaccgccg agttcgtgcg caaccccaac tggcagctgc
     1321 cgggaagcaa tctgcacgac gacatcgcgc aggcttgcgg cgcgggcagc tccgtattca
     1381 tccccgccac cgagctcgcg agcggcctca tgggcgacgc catcgccacc aacatgttca
     1441 tgctcggcta cgcctaccag aagagctgga ttcccgtttc ggcggccgcg ctcgagcgcg
     1501 ccatcgagct gaacggcgtc gcggtagagt tcaaccggaa aagcttcacc tgggggcggc
     1561 gcgcggccgt cgacctcgag cgcgtgcgcc gcatcgccgt accggccgag gtgatatcca
     1621 tcgagcagca tttctcgcgc aacctcgacg agatcgtcga gcggcgcgag aaatttctca
     1681 tcgcctatca ggacgcggcg tacgccgcgc gctaccggca gcttgtggaa aaagtcaaaa
     1741 cgcacgaaaa agaaaagctc ggctcgacca agctcgccga ggccgtggcg cgctactacg
     1801 cgaagctgct cgcctacaag gacgagtacg aagtggcgcg cctgcacgcc gagggcggct
     1861 tcacgaagag gatccgggac atgttcgagg gcgactacca cgtggtgtat cacctcgcgc
     1921 cgccgctgct cgcaggcaaa gatccgctca ccggcgagcc gcgcaagatg cgctttgggc
     1981 cgtggatgct gcccgtgttc aagctgctga agaacctcaa gctgctgcgc ggcacgtcat
     2041 tcgacctgtt cggttacacg caggagcggc gcaccgagcg cgcgctgatc agcgaatacg
     2101 agcagaccgt cgagcgcttg ttgcaaggcc tgtcgccgcg aaatcacgca gtcgccgtcg
     2161 agatcgccac gctcccagag gagatccgcg gcttcggaca catcaagatg aagaacgtcg
     2221 tcgccgttgc aaagaagcgc gacgcgctgc tgcagcgatt cggcagcccc gcggctgagc
     2281 gcgcagcggc ctaaaacagg gacagaccct atttttctca aatgcgaaaa atggggtctg
     2341 tccctatttt tcgttgcagc tgcagctttg gcacagaccg gggagcgcct cctcgcgctc
     2401 gaggatggag ccgtggtgcg ctacaagctg ctcgacgc
//
LOCUS       DASPXW010000020        17178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1140898, whole genome shotgun sequence.
ACCESSION   DASPXW010000020 DASPXW010000000
VERSION     DASPXW010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 17178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17178
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140898"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1405
                     /gene="rpoB"
                     /locus_tag="VEL04_00425"
     CDS             <1..1405
                     /gene="rpoB"
                     /locus_tag="VEL04_00425"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012354150.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HYT46859.1"
                     /translation="KVTPKGETQLTPQEKLLRAIFGEKASDVKDTSLRVPSAISGTVI
                     DVQVFTREGIERDKRSQSIIEDELKRYKTDLADQMRIVESDTFERLERLLTGKTATGG
                     PKRLAKGTKVVKAYLETVEKYDWFEIRLSNEEAAAQVEGLKESLAQKRREFDAMFEAK
                     RKKLTQGDELPPGVLKMVKVYVAVKRRLQPGDKMAGRHGNKGVISKITPVEDMPHMAD
                     GTPVDLVLNPLGVPSRMNVGQILETHLGWAAKGLGLKIGELIKQQQKIAEIKKTIERI
                     YGSSSKDEELSKLTDEEVMQLADNLKEGVPFATPVFDGASEAEINSMLELAGLPASGQ
                     VKLYDGRTGEQFDRDVTVGYMHMLKLHHLVDDKMHARSTGPYSLVTQQPLGGKAQFGG
                     QRFGEMEVWALEAYGAAYTLQEMLTVKSDDITGRTKVYENIVKGDHKIDAGMPESFNV
                     LVKEIRSLGIDIDLERY"
     gene            1405..5607
                     /gene="rpoC"
                     /locus_tag="VEL04_00430"
     CDS             1405..5607
                     /gene="rpoC"
                     /locus_tag="VEL04_00430"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HYT46860.1"
                     /translation="MKALLELFKQVQQDEEFDAIKIGLASPEKIRSWSYGEVKKPETI
                     NYRTFKPERDGLFCAKIFGPIKDYECLCGKYKRLKHRGVICEKCGVEVTLAKVRRERM
                     GHIELASPVAHIWFLKSLPSRLGLVLDMTLRDIERVLYFEAYVVTDPGLTPLKRCQLL
                     TEDDYLAKVEEFGDDFSAAMGAEGIRALLRSLDLNKSLDELRKELEATSSDAKIKKIA
                     KRLKVLEGFKQSNIKPDWMIMEVLPVLPPELRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLELKAPEIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIKGK
                     GGRFRQNLLGKRVDYSGRSVIVVGPQLKLHQCGLPKKMALELFKPFIFNKLELLGLAT
                     TIKAAKRLVEQEVPEVWDILEEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDIVL
                     GLYYGTREHVNAKGEGMSFANLAEVTRAYDSRQVALHARVWVRIRETEVSEDGERSEK
                     VTRYETTIGRALLSEILPAGLPFELLNKPLKKKEISRLINHAFRRCGLRETVIFADKL
                     MQAGFTIATRAGISFGVHDMTIPAQKQELIKAAETEVKEIENQYTSGLVTAGERYNKV
                     VDIWSRCGEQVGKVMMEQLGSEEVENRQGKKVKQEAFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVTQDLVVTEDDCETQNGVSMKALVEGGEVVEALKERILGRVLATDLVHPETQDI
                     LYGAGSLLDEDSVDAIDNLGIDEVKVRTPLSCDTRWGVCAKCYGRDLGRGSLVNVGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRTAVQSHVEAKSAGTVRFTATMRYLSNAKG
                     DKLAISRSGEITIVDDNGRERERHKVPYGATLQVADGDTVRAGKTLASWDPHHRPIIT
                     EYAGTVKFEHVEEGATVAKQIDDVTGLSTLVVIDAKRRTTTTGKGQRPSVKLINEAGD
                     EVRIAGTDHSVNISFPVGAVIAVRDGQQVQVGEVLARIPQEVSKTRDITGGLPRVAEL
                     FEARSPKDAGILAEVTGTVSFGKDTKGKQRLVITDLDKQEHEYLIAKDKHLLVHDGQV
                     VNKGEMIVDGPVDPHDLLRLLGVEELARYIIDEVQDVYRLQGVKINDKHIEVIVRQML
                     RRIEVDDPGDTPFIQGEQVERAELLEENEKAEKDGKKAASFRYMLLGITKASLSTDSF
                     ISAASFQETTRVLTEAAIMGKRDELRGLKENVIVGRLIPAGTGLAYHNIRKAQAAEPV
                     LAELPASAAEAPPAEATTAGGS"
     gene            5639..6973
                     /locus_tag="VEL04_00435"
     CDS             5639..6973
                     /locus_tag="VEL04_00435"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HYT46861.1"
                     /translation="MPAAFSAASPEYQCDVLVIGGGPGGSTISAFLAQKGWKVVLLEK
                     ERHPRFHIGESLLPLNLPLLERLGVLEQVDRIGLVKPGAEFNYEGCEPAVYYFANAMD
                     KSHPWAYEVRRSEFDNVLLRNSAAKGTDVREGVKVTKVEFRPGSTSLVHAVDEHGHAQ
                     CWETRFVVDGSGRDTFLANHFKIKSRNPVHNSSAIFGHFKGAVRRPGRDEGNISVYWF
                     DHGWYWMIPLRDGAMSVGAVCWTYYMKSRKVGVDRFLLDTIALCPAMAARLKDAKLMA
                     PALATGNFSYRATRMTGDGYLMVGDAYAFIDPVFSSGVFLAMNSGELGAGVVDEALRN
                     GDVSARSLRHYDRTVRHGLKTFSWFIYRITTPALRKMFMSPRPAFRLEQAILSLLAAD
                     IFGKTPIRGPLLVFKMIYYVSSIINWRESWVAWRKRRRAWQSKLDFVWKSGN"
     gene            6990..7850
                     /locus_tag="VEL04_00440"
     CDS             6990..7850
                     /locus_tag="VEL04_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HYT46862.1"
                     /translation="MVLQPFRLLSPFALIAGIILAGASAAPAHAEPQPLWEVGIGATG
                     LSLPDYRGSDERRGYLYPFPYLVYRGDALRVDRQGVRGIFFRSERLELDLSIYGTPPV
                     DSSNNATRRGMPDLDPTLELGPLLGLNLMRDRENDRRLDLRFPLRAVIATDLSHAQQA
                     GYVFQPQLNLALRPAFLDGKWNLGMQLGVQYGSRRYHEYFYGVDAQFATPERPAFAAR
                     AGYSGMLALASISRRFSRYWVGGFMRYDTLKGAVFEDSPLLRRQQSTMAGIAFAWIFA
                     QSDRKVEASE"
     gene            complement(7853..8482)
                     /locus_tag="VEL04_00445"
     CDS             complement(7853..8482)
                     /locus_tag="VEL04_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT46863.1"
                     /translation="MLTLLERVLVPSLIVFLLIGGLASVALGWALIFRSAQALSFMRS
                     MNRWVSTRRALKELEVPRHVAEPRKAGRLWFGLFLLAGAGYALYVLLARIEIPRVAVV
                     LGVNLQRWFLASVALQTMKWLLVVGSVLALAVGILMLFFPQVLAAFEARMNRWYSTRQ
                     LLPAGGETMKFPLDLLVEASPRAAGWIIAAASLVVAVAMGILLVARLAG"
     gene            8506..9294
                     /locus_tag="VEL04_00450"
     CDS             8506..9294
                     /locus_tag="VEL04_00450"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF005451.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HYT46864.1"
                     /translation="MGTMRELTSAASAERAVRVDLAAAFRLAVRLELHEGVCNHFSAM
                     LADQEGKRPAESPHKARFLLNRYGLHWSEVAASNLLALDAAGKVLEGEGEIEKTAFYI
                     HSRIHLAHPRAMCVLHTHMPYATTLTLLEGGRLEMVEQNALRFHDDIAYDDTYNGLVV
                     DAAEGNRLARVLGTKRVLFLANHGVMVVGASVAEAFDALYYLERACRLQVLARSMGGK
                     LRAVRSEVVRETYRMMLADAPKYAAAHFSALKRILDREEPGYSR"
     gene            9284..9814
                     /locus_tag="VEL04_00455"
     CDS             9284..9814
                     /locus_tag="VEL04_00455"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYT46865.1"
                     /translation="MLGERVEVRPAAPGDLQAVHAIYAHHVLHGLASFEEIPPAHDEL
                     ARRYQDVVGRGLPWLVADCGGAVAGYGYCAPYRSRSAYRYALEDSVYVREDMQGRGVG
                     TALLAELIRRCEALGYRQLVAVIGDSAHAASINLHTALGFLRVGTLRSVGFKLGRWVD
                     CVIMQRPLGPGDANSP"
     gene            complement(9801..10778)
                     /locus_tag="VEL04_00460"
     CDS             complement(9801..10778)
                     /locus_tag="VEL04_00460"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008647506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HYT46866.1"
                     /translation="MKAVLCKQYGPPESLVVEELPPPRPGRGEAVVSVRAASVNFPDV
                     LIIQNKYQFKPPLPFSPGSELAGVVKELGEGVSGVRPGDRVMAFTTYGAFAEEVKLES
                     ARLVPIPQGMEFTSAAAFLLTYGTSDHALRDRGALQAGETLLVLGASGGVGLAAIEIG
                     KALGARVIACASSEDKLAVCRSHGADATIDYAREDLRERVKALTDARGPDVIYDAVGG
                     PYTEPAFRSIAWRGRLLVVGFAAGEIPKLPLNLALLKGASVVGVFWGDFARREPERFA
                     DSLRRLARWYGEGKLRPHVSQTFPLEKAAEALKLMAARQVKGKVVLTVS"
     gene            complement(10795..11196)
                     /locus_tag="VEL04_00465"
     CDS             complement(10795..11196)
                     /locus_tag="VEL04_00465"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HYT46867.1"
                     /translation="MRAVDTNVVVRFLTGDDAAQFRRARRVMQSGTVFIPKTVLLETE
                     WVLRRAYSIAPEEILHALDSLAHAAEVVIEDETAIAQALLWYRDGMDFADALHIASRG
                     AATQFLTFDRRLTSAARRAGIKGVTQPGLSR"
     gene            complement(11177..11446)
                     /locus_tag="VEL04_00470"
     CDS             complement(11177..11446)
                     /locus_tag="VEL04_00470"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HYT46868.1"
                     /translation="MDTTKLSAKGQVVLPKGVRDAHGWNPGVEFVVESTSDGVLLRPK
                     ARKKSSRVAELAGMLKRKGRKRVSLGTMAAAIESEILARRARGRY"
     gene            11546..12436
                     /locus_tag="VEL04_00475"
     CDS             11546..12436
                     /locus_tag="VEL04_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYT46869.1"
                     /translation="MPTLTTDDGVKLYYEEAGAGTPIVFVHEFAGDARSWEPQLRYFS
                     RRYRCIAYNARGYPPSDVPEDFGRYSQARARDDIRCALDALGLQRAHIVGLSMGAFAT
                     LHFGISYGARALSLVVAGGGYGAHPAQYAQFQKDSKAHAEFIRREGMAKFAATYGRGA
                     TRIQFENKDPRGFAEFIAQLAGHSAAGSANTMLGYQARRPSLYDLTAEMGRIEAPTLI
                     MAGDEEEPCLEVCLLMKRAIRSAGLAILPKSGHGINLEEPALFNQLLDDFFRQVETGR
                     WAARDPRAVVASIYGPSGKP"
     gene            12433..13212
                     /locus_tag="VEL04_00480"
     CDS             12433..13212
                     /locus_tag="VEL04_00480"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HYT46870.1"
                     /translation="MSLVVEKRGAVGWVIFDQPAKRNAINGAMWRGIAPAMQQFDADA
                     QVRCVAFRGAGSGAFSAGADISEFEAVRAQRASVSQYDGLLDQVLHAIQDSPKPSLAM
                     IHGYCMGGGLEVALACDLRYCGASAQFGIPAAKLGLAYNIEGHKRLLETVGHARAREI
                     MFLGRRYPAGEALAMGLVHRVLPDSALENFVMEVLQTLSENAPLSIANSKTIIEEYVK
                     SAGAADAARMHAAIDRCARSEDYEEGRRAFVEKRKPLFKGK"
     gene            13265..14350
                     /locus_tag="VEL04_00485"
     CDS             13265..14350
                     /locus_tag="VEL04_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HYT46871.1"
                     /translation="MKLRVRSIRARAVSAPMKRPLATSTGQVSSAALLLIDLQSDAGI
                     VGRSYLFGIGRHNLAPMARLVEAMAQMIEGDECAPLEIERKLRARYTLLGVHNMVLFA
                     MAGIDMAAWDVFGQALGQPLARLLGAAPRPIPAYNSKGLGILPIKDLVKQAAELVGEG
                     FRAVKLRLGRADAHADLDALRAVKQAIGGKIDLMVDYNQALSVAEAMRRGRMLDDEGG
                     VYWIEEPVRADDFAGCARVAREVATPIQIGENFMGPEQMAQALAAGACDYVMPDAQRI
                     GGVTGWMRAAALAQGAGVEMSSHLFPEASCHLLAATPTCHWLEFVDWAEPVLKHPVEL
                     KNGHVVIPTRPGIGMTWDEKAVKRYAI"
     gene            14377..16005
                     /locus_tag="VEL04_00490"
     CDS             14377..16005
                     /locus_tag="VEL04_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HYT46872.1"
                     /translation="MFSRHYPHWPPGLPKNFPVPQASVYENLAHCAAQHPDRPAIHYY
                     GTDVTYGELKREVDALAGFLQQRCGVRKGDRVLLYLQNSPQFVIGYYAILRADAVAVP
                     VNPMNLTGELRHYVEDAGAGTAIVGQELAARIAPLGLRLVVAAYSDYIRQPTDLPLPE
                     FVRAPRESASWNEAIGARLEPGPHQAKSDDLAAMPYTSGTTGKPKGCMHTHGSIQATT
                     VAYLEWRGSGDGSIVLSALPMFHVTGMQAGMNGPLCKGATLVVMSRWDRDCAAMLIER
                     LGVTNWSAITTMLVDFLSNSALKKYDLSSLKVLGGGGAAMPEALARKLEEVFGLPYVE
                     GYGLSETMAPTHINPPQRAKRQCGGIPIFNTDARIVDLESLKELGPNEVGEIIVHGPQ
                     VFKGYWKQDEATRQAFVEHDGKRFFRTGDLGYYDEEGYFFITDRLKRMINASGYKVWP
                     AEVEALLYAHPDIQEACVIGTPDGYRGESVKAVIVLKEASRKRLSPEDISEWARGRMA
                     AFKVPRVVEFVEQLPKTPTGKILWRELQEKERRK"
     gene            16002..16442
                     /locus_tag="VEL04_00495"
     CDS             16002..16442
                     /locus_tag="VEL04_00495"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HYT46873.1"
                     /translation="MRHALFVLCIVATPVLGQVNIENAWARATAPGAKLGAGYMTIRN
                     ASGEPDRLVAASSPRAQRVEMHVHLKDGEVMKMREVKGFDIPPRGGFELKPGGAHLMF
                     VSIGAPFKEGEKIPVKLRFQRAGEVKTEFQVGRLADAGPPHGKH"
     gene            complement(16439..>17178)
                     /locus_tag="VEL04_00500"
     CDS             complement(16439..>17178)
                     /locus_tag="VEL04_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003266172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HYT46874.1"
                     /translation="AIAWDIITWYYGIPSSSSHALIGGIIGAGVAKGGMWILVPAGVS
                     KIFLFIILSPLLGFALSSMMMLAVAWLFRRSAPSKVDRWFRRMQLVSASFYSLGHGGN
                     DAQKTAGIIWMLLIAAGYYGAKDPFPLWVAVACYITIALGTLFGGWRIVKTMGQKITK
                     LRPVGGFCAETGGAITLFTTGFLGIPVSTTHTITGAIIGVGAAQSVSAVRWGIAGNIV
                     WAWILTIPCSAFIAALAWAAARALFAG"
BASE COUNT         3206 a         5534 c         5705 g         2733 t
ORIGIN      
        1 caaggtgacg ccgaaaggcg agacccagct cacgccgcaa gagaagctgc tgcgcgcgat
       61 ttttggagaa aaagcgagcg atgtcaaaga cacatcgctg cgcgtgccat ccgcgatctc
      121 gggcacggtg atcgacgtcc aggtgttcac gcgcgaaggc atcgagcgcg acaagcgcag
      181 ccagagcatc atcgaggacg agctcaagcg ctacaagacc gacctcgccg accagatgag
      241 gatcgtggaa agcgatacct tcgagcgcct cgagcgcctg ctcaccggca agaccgcgac
      301 cggcgggccg aagcgcctgg cgaagggcac caaggtggtg aaggcctatc tcgagaccgt
      361 cgagaaatac gactggttcg aaatccgcct gtcgaacgag gaggcggcgg cgcaggtcga
      421 ggggctgaag gagtcgctgg cgcagaagcg ccgcgagttc gacgccatgt tcgaagccaa
      481 gagaaagaag ctcacgcagg gcgacgagct gccgccgggc gtgctgaaaa tggtcaaggt
      541 ctacgtcgcg gtgaagcgcc gcctgcagcc cggcgacaag atggccgggc gccacggcaa
      601 caagggcgtg atctcgaaga tcaccccggt ggaggacatg ccgcacatgg ccgacggcac
      661 gccggtagac ctggtgctga acccattggg agtgccttcg cgcatgaacg tggggcagat
      721 cctcgagact cacctcggct gggcggcgaa gggcctgggt ttgaagatcg gcgagctcat
      781 caagcagcag caaaaaatcg ccgagatcaa gaagacgatc gagcgcatct atggctcgag
      841 cagcaaggac gaggagctct ccaagctgac cgacgaggaa gtgatgcagc tcgccgacaa
      901 cctcaaggag ggcgtgccgt tcgcgacgcc ggtgttcgac ggcgcctccg aggccgagat
      961 caattccatg ctcgagctgg cggggctgcc ggcgtcgggg caggtgaagc tctacgacgg
     1021 ccgcaccggc gagcagttcg accgcgacgt gaccgtcggc tacatgcaca tgctcaagct
     1081 ccatcacctg gtcgacgaca agatgcacgc gcgctccacc gggccgtaca gcctggtgac
     1141 gcagcagccg ctcggcggca aggcgcagtt cggcggccag cgcttcggcg agatggaagt
     1201 gtgggcgctg gaagcctacg gcgcggcgta cacgctgcaa gagatgctga cggtgaaatc
     1261 cgacgacatc accggccgca ccaaggtgta cgagaacatc gtcaagggcg accacaagat
     1321 cgacgcgggc atgcccgagt cgttcaacgt gctggtgaag gaaatccgct cgctcggcat
     1381 cgacatcgat ctggagaggt actgatgaaa gcgcttctag agctcttcaa gcaggtccag
     1441 caggacgagg agttcgacgc catcaagatc gggctcgcct cgcccgagaa gatccgttcg
     1501 tggtcgtacg gcgaagtgaa gaaacccgag accatcaact accgcacctt caagccggag
     1561 cgcgacggcc tgttctgcgc caagatcttc gggccgatca aggactacga gtgcctttgc
     1621 ggcaagtaca agcgcctcaa gcaccgcggc gtcatctgcg agaaatgcgg cgtcgaagtc
     1681 acgctggcga aagtgcgccg cgagcgcatg ggccacatcg agctcgccag cccggtggcg
     1741 cacatctggt tcctgaagtc gctgccttcg cgtttgggtc tggttctcga catgacgctg
     1801 cgcgacatcg agcgcgtgct gtatttcgag gcctatgtcg tcaccgaccc cggccttacg
     1861 ccgctcaagc gctgccagct tctcaccgag gacgactatc tcgccaaggt cgaggagttc
     1921 ggcgacgact tctccgcggc gatgggcgcc gagggcatcc gcgcgctgct gcgctcgctc
     1981 gacctgaaca aatcgctcga cgagctgcgc aaggagctcg aggcgacctc gtcggacgcc
     2041 aagatcaaga agatcgccaa gcgcctcaag gtgctcgagg gcttcaagca gtcgaacatc
     2101 aagcccgact ggatgatcat ggaggtgctg ccggtgctgc cgcccgagct gcgccctctg
     2161 gtccctcttg atgggggccg atttgccacc tcagacctca atgatcttta cagaagggtg
     2221 atcaacagga acaacaggct gaaaaggctg ttggaactga aggcgccgga aatcatcgtg
     2281 cgcaacgaga agcgcatgct gcaggaagcg gtggactcgc tgctcgacaa cggccgccgc
     2341 ggcaaggcga tgaccggcgc caacaagcgg ccgctgaaat cgcttgccga catgatcaag
     2401 ggaaaaggcg gacggttccg gcaaaaccta ttaggcaagc gagtcgatta ttccggacgc
     2461 tcggtgatcg tggtcggccc gcagctgaag ctgcaccagt gcggcctgcc gaagaagatg
     2521 gcgctggagc tcttcaagcc ttttattttt aataaattgg aattgttagg actggctacg
     2581 actatcaagg cggccaagcg cctggtggag caggaggtgc ccgaggtctg ggacatcctc
     2641 gaggaggtga tccgcgagca tccggtgcta ttgaaccgtg cgccgacatt gcatcgcctc
     2701 ggcatccagg ctttcgagcc ggtgctgatc gagggcaagg cgatccagct ccatccgctg
     2761 gtgtgcgcgg cattcaacgc ggacttcgac ggcgaccaga tggcggtgca cgtgccgctg
     2821 tcgctcgagg cgcaaatgga ggcacgcacg ctgatgctcg cctcgaacaa cgtgctgtcg
     2881 cccgccaacg gcgagccgat catcgtgcct tcgcaggaca tcgtgctggg gctgtattac
     2941 ggcacgcgcg agcacgtgaa cgcgaagggc gaggggatga gcttcgccaa cctcgccgag
     3001 gtgacgcgcg cgtacgactc gcgccaggtc gccttgcatg cccgcgtctg ggtgcgcatc
     3061 cgggaaaccg aggtcagcga agacggcgag cgcagcgaga aagtgacgcg ctacgagacc
     3121 accatcggcc gggcgctgct ctccgagatc ctgcccgccg ggctgccgtt cgagctgctg
     3181 aacaagccgc tcaagaagaa ggagatctcg cggctgatca accacgcctt ccggcgctgc
     3241 ggcctgcgcg agacggtgat cttcgccgac aagctgatgc aggcgggctt caccatcgcc
     3301 acgcgcgccg gcatttcctt cggcgtgcac gacatgacca tcccggcgca gaagcaggag
     3361 ctgatcaagg ccgccgagac cgaggtgaag gagatcgaga accagtacac ctcggggctg
     3421 gtcaccgccg gcgagcgcta caacaaggtg gtcgacatct ggtcgcgctg cggcgagcag
     3481 gtcggaaaag tcatgatgga gcagctcggc agcgaggaag tcgagaaccg ccagggcaag
     3541 aaagtgaagc aggaggcgtt caactcgatc tacatgatgg ccgactcggg cgcgcgcggc
     3601 tcggcggctc aaatcagaca gctggccggg atgcgcggcc tgatggcgaa gcccgacggc
     3661 tcgatcatcg agacgccgat caccgcgaac ttccgcgaag ggctgaacgt gctgcagtac
     3721 ttcatctcga cgcacggcgc gcgcaagggc ctcgccgaca ccgcgctgaa gaccgcgaac
     3781 tccggttatc tcacgcggcg cctggtcgac gtcacccagg acctggtggt caccgaggac
     3841 gactgcgaga cccagaacgg ggtctcgatg aaggcgctgg tcgagggcgg cgaggtggtc
     3901 gaggcgctca aggagcgcat tctcggccgg gtgctggcaa cggatctggt gcatccggag
     3961 acgcaggaca ttctctacgg cgcgggcagc ctgctcgacg aggattcggt cgacgcgatc
     4021 gacaatctcg gcatcgacga agtcaaggtg cgtaccccgc tttcctgcga cacgcgctgg
     4081 ggcgtgtgcg ccaagtgcta cgggcgcgat ctcggccgcg gctcgctggt gaatgtcggc
     4141 gaggcaatcg gcgtgatcgc ggcgcaatcg atcggcgagc cagggacgca gctcacgatg
     4201 cgcaccttcc acatcggcgg cgccgcctcg cgcaccgcgg tgcaaagcca tgtcgaggcg
     4261 aagtcagccg ggacagtgcg cttcaccgcg accatgcgct acctctccaa cgcgaagggc
     4321 gacaagctgg cgatctcgcg ctcaggcgag atcacgatcg tcgacgacaa cggccgcgag
     4381 cgcgagcgcc acaaggtgcc ttacggcgcg acgctgcagg tcgccgacgg cgatacggtg
     4441 cgcgccggca agacgctcgc cagctgggat ccgcaccacc ggccgatcat caccgaatac
     4501 gccggcacgg tgaagttcga gcacgtcgag gaaggcgcca ccgtcgccaa gcagatcgac
     4561 gacgtgacgg ggctctccac gctggtggtg atcgacgcca agcggcgcac cacgaccacc
     4621 ggcaagggcc agcggccttc ggtgaagctg atcaacgaag cgggcgacga agtgcgcatc
     4681 gcgggcacgg atcactccgt gaacatcagc ttccccgtcg gtgccgtgat cgcagtgcgc
     4741 gacgggcagc aggtgcaggt gggcgaggtg ctcgcccgca tcccccagga ggtttcgaag
     4801 acgcgcgaca tcaccggcgg cctgccgagg gtggcggagc tgttcgaagc gcgctcgccc
     4861 aaagacgcgg ggatccttgc cgaagtgaca ggaaccgtga gcttcggcaa agatacgaaa
     4921 ggcaagcagc gcctggtgat tacggacctc gacaagcagg agcacgaata cctgatcgcc
     4981 aaggacaagc acctgctggt gcacgacggc caggtggtca acaagggcga gatgatcgtt
     5041 gacggcccgg tcgatccgca cgacctgctg cggctgctcg gcgtcgagga gctggcgcgc
     5101 tacatcatcg acgaggtgca ggacgtctac cgcctgcagg gcgtcaagat caacgacaag
     5161 cacatcgagg tgatcgtgcg gcagatgctg cggcgcatcg aggtggatga tccgggcgac
     5221 acgcccttta ttcagggcga gcaggtggag cgcgccgagc tgctcgagga aaacgagaaa
     5281 gccgaaaagg acggcaagaa ggcggccagc ttccgctaca tgctgctcgg catcaccaag
     5341 gcgtcgctct cgaccgactc gttcatctcg gcggcctcct tccaggagac cacgcgcgtg
     5401 ctcaccgagg cggcgatcat gggcaagcgc gacgagctgc gcgggctgaa agagaacgtg
     5461 atcgtcgggc gcctcatccc ggcgggcacg ggactcgcgt atcacaacat ccgcaaggcg
     5521 caggcggccg agccggtgct ggccgagctg ccggcaagcg cggctgaggc gcctccggcg
     5581 gaagcgacca cagccggggg aagttaacgg cccggagaat ccaaggcgct cgcacagcat
     5641 gcctgctgct tttagtgccg cttcgccgga ataccagtgc gatgtgctgg tcatcggcgg
     5701 cgggccgggc ggctcgacca tctcggcgtt tctggcgcaa aagggctgga aggtcgtgct
     5761 gctggagaag gaacgtcatc cccgcttcca catcggcgaa tcgctgctgc cgctcaatct
     5821 gccgttgctc gagcgcctgg gggtgctcga gcaagtggac cgcatcgggc tggtcaagcc
     5881 cggcgccgag ttcaattacg aaggctgcga gccggccgtc tattatttcg ccaacgccat
     5941 ggacaagtcc cacccctggg cttacgaggt gcggcgttcg gagttcgaca acgtattgct
     6001 gcggaacagc gccgccaagg gcaccgacgt gcgcgagggc gtcaaagtca cgaaagtgga
     6061 attccggccg ggctcgacct cgctcgtgca tgccgtggat gagcacggcc acgcgcaatg
     6121 ctgggagacg cgttttgtgg tggatggctc cgggcgtgac actttcctcg ccaatcactt
     6181 caagatcaag agccgcaacc cggtgcacaa cagctccgcc attttcggcc atttcaaagg
     6241 agcagtgcgc cgcccgggcc gcgacgaggg caacatcagc gtctattggt tcgatcacgg
     6301 ctggtactgg atgattccgc tgcgcgacgg cgcgatgagc gtcggtgcag tgtgctggac
     6361 gtattacatg aagagccgca aggtcggcgt ggatcgattc ctgctcgaca ccatcgcgct
     6421 gtgcccggcc atggccgcgc gcctgaaaga cgcgaagctg atggcacccg cgctcgcgac
     6481 gggcaatttc tcgtatcgcg ccacgcgcat gaccggcgac ggctatctga tggtcgggga
     6541 cgcctatgcg ttcattgatc cggtgttctc gagcggcgtg ttcctcgcca tgaacagcgg
     6601 cgagctgggc gccggagtcg tggacgaggc cttacgcaac ggcgatgtat cggcgcgcag
     6661 cctgcggcac tacgatcgga ccgtccggca cggcctcaag acattctcct ggttcatcta
     6721 ccgcatcact accccggcgc tgcgcaagat gttcatgtcg ccgcgccctg cgttccgttt
     6781 ggaacaggcg atcctgtcgc tgcttgcggc ggacatcttc ggcaagacac cgatccgggg
     6841 ccctctcctg gtcttcaaga tgatttacta cgtttcctcg atcatcaact ggcgcgagag
     6901 ctgggtcgcc tggaggaagc gcagacgtgc ttggcagtcg aagctcgatt ttgtctggaa
     6961 gagcggtaac tgagagaccc ggccggcgca tggtcctcca gccgttccgg ctgctgtctc
     7021 cgtttgcgtt aatcgctgga atcatcctgg ccggcgcatc ggctgcgccg gcgcatgccg
     7081 agccgcagcc gttgtgggag gtggggatcg gcgccaccgg gctcagcctg cccgactacc
     7141 gtggctccga cgaacgtcgc ggctacctct acccctttcc ctacctggtt tatcgaggcg
     7201 atgcgctgcg cgtcgaccgc cagggcgtgc gcggcatttt cttccgctcg gagcgcctcg
     7261 agctcgacct cagcatctac ggcacgcctc cggtggacag ctccaacaat gccacgcgca
     7321 gaggcatgcc ggacctcgat ccgaccctcg agctcggtcc gcttctgggc ctcaacctga
     7381 tgcgcgaccg ggagaacgat cgccggctcg atctgcggtt tccgctgcgc gcagtgatcg
     7441 cgacggacct ttcgcatgca cagcaggcag gctatgtgtt ccagccgcag ctcaatctcg
     7501 cgctgcgccc ggcattcctg gacggcaagt ggaacctcgg catgcagctc ggcgtgcagt
     7561 acggctcgcg ccgataccat gaatatttct atggtgtgga cgcccaattc gccacgccgg
     7621 agcggccggc ctttgcagcg cgcgccgggt actccggcat gctcgcgctc gcctcgatct
     7681 cacgccgctt cagccgttac tgggtcggcg gcttcatgcg ttatgacacg ctcaagggcg
     7741 ccgtgtttga agacagcccg ctgctgaggc gccagcagag caccatggca ggcatagcct
     7801 tcgcctggat ctttgcgcag tccgaccgca aggtcgaagc gagcgagtaa ggtcagcccg
     7861 cgagccgcgc aaccagcagg atgcccatcg cgacggcgac gacaagcgac gcggcggcga
     7921 tgatccaacc cgcggcgcgg ggggaagcct cgaccagcaa gtcgagcggg aatttcatgg
     7981 tttcgccgcc ggcgggcagc agctggcggg tcgaatacca gcggttcatg cgcgcttcaa
     8041 acgccgcgag gacctgcggg aaaaacagca tcagaatccc gaccgccaag gcgagcacgc
     8101 tgccgacgac cagcagccat ttcatggtct gcaacgcgac actggcgagg aaccagcgct
     8161 ggaggttcac gccgagcacc accgccacgc gcggaatctc gatccgggcg agcaggacgt
     8221 acagcgcata gccggcgccg gcgagcagga acagtccgaa ccagagcctg cccgccttgc
     8281 gtggttccgc cacatggcgc ggcacctcga gctccttgag cgcgcgccgc gtcgacaccc
     8341 agcggttcat gctgcgcatg aagctcagcg cctgcgcgga gcgaaaaatc agcgcccagc
     8401 caagcgccac gctggcgagg ccgccgatca gcagaaacac gatcagcgac ggcaccagca
     8461 cgcgttcgag cagggtgagc acggtcagcc actataatcc aatgcatggg gacgatgcgc
     8521 gagcttacca gcgccgccag cgccgagcgc gccgtgcggg tcgacctcgc ggccgccttc
     8581 cggctcgcgg tgcgcctcga gctgcacgag ggcgtgtgca atcatttcag cgccatgctg
     8641 gcggaccagg aagggaaacg tccggcagag tcccctcaca aggcgcgatt cctgctcaac
     8701 cgctacgggc tgcactggag cgaggtcgcc gcctccaacc tgctcgccct cgatgcggcg
     8761 ggcaaggttc tcgaaggcga gggcgagatc gagaaaacgg cgttctacat ccactcgcgc
     8821 atccatctcg cgcatccccg cgccatgtgc gtgctgcaca cccacatgcc gtacgccacc
     8881 acgctcacgc tgctcgaagg cggcagactc gagatggtcg agcagaatgc gcttcgcttc
     8941 cacgacgaca tcgcctacga cgacacctac aacgggctgg tggtcgacgc tgcggaaggc
     9001 aaccggctgg cgcgcgtgct cggcacgaag cgggtgctgt ttctcgcgaa ccatggcgtg
     9061 atggtggtcg gcgcctcggt cgccgaagcg ttcgatgcgc tctactacct cgagcgcgcg
     9121 tgccggctgc aggtgctcgc acgctccatg gggggaaagc tgcgcgcggt gcgcagcgag
     9181 gtggtgcgcg agacctaccg catgatgctc gccgacgcgc cgaaatacgc cgctgcgcac
     9241 ttcagcgcgc tcaagcgcat cctcgaccgc gaggagcccg gttattctcg gtgagcgggt
     9301 cgaggtgcgg cccgccgcgc cgggcgatct gcaggccgtg cacgcgatct acgcgcatca
     9361 cgtgctgcac ggtctcgcct ctttcgagga aatcccgccg gcgcacgacg agctcgcacg
     9421 ccgctaccag gacgtagtcg gtcgcggcct gccctggctg gtcgccgatt gcggcggcgc
     9481 ggtggcggga tacggttatt gcgccccgta ccgctcgcgc tcggcgtacc gctatgcgct
     9541 cgaggactcg gtctacgtgc gtgaggacat gcagggtcgg ggcgtcggca ccgcgctgct
     9601 cgcggagctg atccgccgct gcgaagcgct cggctaccgc cagctcgttg cggtgatcgg
     9661 cgacagcgcg cacgccgctt cgatcaacct gcacaccgca ctcggcttcc tgcgcgtcgg
     9721 cacgctgcgc tcggtcggat tcaagctcgg ccgctgggtc gactgcgtga tcatgcagcg
     9781 cccgctcggc ccgggggacg ctaactcacc gtaagcacca ccttgccctt aacctggcgc
     9841 gcggccatca gcttgagcgc ctcggcggct ttttccagcg ggaaggtctg cgagacatga
     9901 gggcggagct tgccctcgcc gtaccagcgc gcgagccggc gcaggctgtc ggcgaagcgc
     9961 tcgggttcgc ggcgcgcgaa atctccccag aacacgccga ccaccgacgc gcccttgagg
    10021 agcgcgaggt tgagcggcag cttcgggatc tcgccggcgg caaagccgac caccagcagc
    10081 cggccgcgcc aggcgatcga gcggaatgca ggctcggtat aaggtccgcc gaccgcgtcg
    10141 tagatgacgt cggggccgcg ggcatcggtg agcgccttga cgcgctcgcg cagatcctcc
    10201 ctggcgtaat cgatggtcgc gtcggctccg tggctgcggc agacggcgag cttgtcttcg
    10261 ctcgaggcgc aggcaatgac gcgcgcgccc agggccttgc cgatctcgat ggccgcgagt
    10321 ccgaccccgc ccgaagcgcc tagcaccaag agggtctcgc cggcctgcag cgcgccgcgg
    10381 tcgcgcagcg cgtggtcgga ggtgccgtag gtgagcagga acgccgcggc ggaggtgaat
    10441 tccatgcctt gcgggatcgg caccaggcgc gccgactcga gcttcacttc ctcggcgaat
    10501 gcgccgtagg tcgtgaaggc catcacccgg tcgcccggcc gcacgccgct cacgccttcg
    10561 cccagctcct tcaccacgcc cgccagctcg ctcccgggcg agaacggcag cggcggcttg
    10621 aactggtact tgttctgaat gatgaggaca tcggggaagt tgacgctcgc ggcgcgcacc
    10681 gacaccactg cctcgccgcg acccgggcgc ggtggaggca gctcctcgac caccagcgat
    10741 tccggcgggc cgtactgctt gcagagtacc gctttcatcg cgtttccaac ttattcatcg
    10801 gcttaagccg ggttgagtaa cgcccttgat gccggctcta cgggctgcgg aggtcaagcg
    10861 cctgtcgaag gtaagaaatt gcgtcgcagc acctcgtgag gcgatatgca gggcatcggc
    10921 gaagtccatc ccatcgcggt accacagtag cgcctgcgca atcgcggttt catcctcgat
    10981 gacgacttcg gccgcgtgtg cgagcgagtc cagggcgtgc agaatttctt ccggcgctat
    11041 cgaataagct cgccgcagga cccattcggt ttccagaaga accgtcttcg ggatgaaaac
    11101 ggtgccgctt tgcattaccc gccgcgcgcg tctaaactgc gcggcgtcat ccccggtgag
    11161 gaagcggact acgacattag tatcgaccgc gcgcacgccg ggcgagaatc tcggattcaa
    11221 tcgcggcggc catggttcct agcgacactc ttttcctccc cttgcgtttg agcatgccgg
    11281 ccaactctgc gacgcggctc gacttttttc ttgctttagg gcgcagcaga acgccgtcgg
    11341 atgtcgactc gaccacgaac tcaacgcccg ggttccagcc atgagcatcg cgaacgcctt
    11401 tcggcaggac cacctgcccc ttggctgaga gcttcgtcgt atccatcgcc ggtaccggta
    11461 agacgctagt aagatatcct aacgcgcgca gtgcccgccg gcaagcgctt ccctttgagg
    11521 ctcagctcca tataggcttc acactgtgcc aacgctaacc accgacgacg gcgtcaagct
    11581 ctattacgag gaagcgggcg ccggcacccc gatcgttttc gtccacgagt tcgccggcga
    11641 tgcgcgcagc tgggagccgc agctgcgcta tttctcgcgc cgctaccgct gcatcgcgta
    11701 caacgcgcgc ggctatccgc cctcggacgt gcccgaggat ttcgggcgct actcgcaggc
    11761 gcgcgcgcgc gacgacatcc gctgcgcgct cgatgcgctc ggcctgcagc gcgcgcacat
    11821 cgtcggcctg tcgatgggcg cgttcgccac gctgcacttc ggcatcagct atggcgcgcg
    11881 cgcgttgtcg ctggtcgtcg ccggcggcgg ctacggcgcg catcccgcgc aatatgcgca
    11941 gttccagaag gactccaagg cgcacgccga attcatccgc cgcgaaggca tggcgaaatt
    12001 cgccgccacc tacggccgcg gcgcgacgcg catccagttc gagaacaagg acccgcgcgg
    12061 cttcgccgag tttattgcgc agctggccgg gcactcggcg gcaggctcgg cgaacaccat
    12121 gctcggctat caggcgcgcc gcccttcgct ctacgacctg accgccgaga tggggcgcat
    12181 cgaggcgccg acgctcatca tggcgggcga cgaggaagaa ccctgcctcg aagtgtgcct
    12241 gctgatgaag cgcgccatcc gcagcgccgg gctggcgatc ctgccgaaga gcggccatgg
    12301 catcaacctg gaggagccgg cgctcttcaa ccagctgctc gacgatttct tccgccaggt
    12361 cgagaccggg cgctgggccg cgcgcgaccc gcgcgcggtg gttgcgtcga tctacggccc
    12421 gtcaggcaaa ccttgagtct agtcgtcgaa aagcgcggcg ccgtcggctg ggtgatcttc
    12481 gaccagccgg cgaagcgcaa cgcgatcaac ggcgcgatgt ggcgcggcat cgcacccgcc
    12541 atgcagcagt tcgatgccga tgcgcaggtg cgttgcgtgg catttcgcgg cgccggcagc
    12601 ggcgctttct cggccggcgc cgacatctcc gagttcgagg ccgtccgcgc gcagcgcgcc
    12661 tcggtctcgc aatacgacgg gctcctcgac caggtgctgc acgccatcca ggattcgccc
    12721 aagccttcgc tggccatgat ccatggctat tgcatgggag gcggcctgga agtcgcgctc
    12781 gcctgcgacc tgcgctattg cggcgcctcg gcgcagttcg gcattcccgc ggcgaagctc
    12841 ggccttgcct acaacatcga aggccacaag cggctgctcg agaccgtcgg ccatgcgcgc
    12901 gcgcgggaga tcatgtttct cggccggcgc tacccggccg gagaggcctt ggcaatgggc
    12961 ctggtgcacc gggtgttgcc tgattccgct ttggaaaatt tcgtgatgga agttctgcaa
    13021 acattatctg aaaatgcacc gctctcgatc gcgaactcga agaccatcat cgaggaatac
    13081 gtgaaatcgg cgggcgccgc ggacgctgcg cgcatgcacg cggcgatcga tcgctgcgcg
    13141 agaagcgaag actacgaaga aggccggcgc gccttcgtgg aaaagcgcaa gccgctattc
    13201 aagggaaaat gaaggggatt tatttataaa tccaaaatga aggggattta tttataaatc
    13261 ccaaatgaag ctcagggtcc gcagcatccg cgcccgcgcg gtgtcggcgc cgatgaagcg
    13321 cccgctcgcc accagcaccg ggcaagtgag cagcgccgcg ctcctgctca tcgatttgca
    13381 gagcgacgcc ggaatcgtcg ggcgctccta cctgttcggc atcggcaggc acaacctggc
    13441 gccgatggca aggctggtcg aggcgatggc gcagatgatc gaaggcgacg agtgcgcgcc
    13501 gctcgaaatc gagcgcaagc tgcgcgcgag gtacacgctg ctcggcgtgc acaacatggt
    13561 gctgttcgcg atggccggga tcgacatggc ggcctgggac gtattcgggc aggcgctcgg
    13621 ccagccgctc gcgcggctgc tcggtgccgc gccgcggccg atccccgcct acaactccaa
    13681 gggcctcggc atcctgccga taaaggacct ggtgaaacag gcggccgagc tggtgggcga
    13741 aggcttccgg gcggtgaagc tgcggctcgg acgtgccgat gcgcatgcgg atctggacgc
    13801 cctgcgcgcc gtgaagcagg cgattggagg caaaatcgat ctgatggtcg attacaacca
    13861 ggcgctgagc gtcgccgagg cgatgcgccg cggccgcatg ctcgacgacg agggcggcgt
    13921 ctactggatc gaggagccgg tgcgcgccga cgacttcgcg gggtgcgccc gcgtagcccg
    13981 cgaagtcgcg acgccgattc aaatcggcga gaacttcatg ggccccgagc agatggcgca
    14041 ggcgctcgcc gccggcgcct gcgattacgt gatgcccgat gcgcagcgca tcggcggtgt
    14101 caccggctgg atgcgcgccg cggcgctcgc gcagggcgcg ggcgtcgaga tgtcgagcca
    14161 cctgtttccc gaggcgagct gccatctgct cgccgcgacg ccgacctgcc actggctcga
    14221 gttcgtcgat tgggccgaac cggtgctgaa gcatcccgtc gagctgaaaa acggccacgt
    14281 cgtcatcccg acccggcccg gcatcggcat gacctgggac gaaaaagcgg tgaagcgcta
    14341 cgcgatctga tgttcacgcg gcttctagcg tcctagatgt tcagccggca ctacccgcat
    14401 tggccgcccg ggctgccgaa gaatttcccg gtgccgcagg cgagcgtcta cgagaacctg
    14461 gcgcactgcg ccgcgcagca tcccgaccgg ccggcgatcc actattacgg caccgatgtt
    14521 acctacggcg agctgaagcg cgaggtcgat gccctggcgg ggtttctcca gcagcgctgc
    14581 ggggtgcgca agggcgaccg cgtgctgctc tatctgcaga atagccccca gttcgtcatc
    14641 ggctactacg ccatcctgcg cgccgacgcg gtagcggtgc cggtgaaccc gatgaacctc
    14701 accggggagc tgcgccacta cgtcgaggac gccggggcgg gcaccgccat tgtcggccag
    14761 gagctcgcgg cgcgtattgc acccctggga ctgcgccttg ttgtcgccgc gtactccgac
    14821 tacatccgcc agccgaccga tctgccgctg cccgagtttg tgcgcgcgcc ccgcgagtcg
    14881 gcttcgtgga acgaagcgat cggggcgcgc ctcgagcccg gtccgcacca ggcgaaaagc
    14941 gacgacctcg cggcgatgcc ctatacctcg gggaccacgg gcaagccgaa aggctgcatg
    15001 catacgcacg gcagcatcca ggcgaccacg gtggcgtacc tcgagtggcg cggcagcggc
    15061 gacggctcga tcgtgctgag cgcgctgccg atgttccatg tcaccggcat gcaggcggga
    15121 atgaacggcc cgctgtgcaa gggcgcgacg ctggtcgtga tgtcgcgctg ggaccgggat
    15181 tgcgccgcga tgctgatcga gcgcttgggg gttacgaact ggtcggcgat cacgacgatg
    15241 ctggtcgatt ttttatcgaa cagtgccctg aaaaaatatg atctgtcgtc gctcaaggtg
    15301 ctcggcggcg gcggcgcggc catgcccgaa gcgctcgcgc gcaagctcga ggaggtgttc
    15361 gggctgccgt acgtcgaggg ctacgggctc tcggaaacga tggcgccgac gcacatcaat
    15421 ccgccgcagc gcgcgaagcg gcagtgcggc ggcatcccca ttttcaacac ggatgcacgc
    15481 atcgttgacc tcgaatccct gaaggaactc ggccccaacg aggtgggaga gatcatcgtc
    15541 cacggtcccc aggtgttcaa gggctactgg aagcaggacg aggcgacgcg gcaggctttc
    15601 gtcgagcacg acggcaagcg cttctttcgc accggcgatc tcggctatta cgacgaggaa
    15661 ggctatttct tcatcaccga ccgcctcaag cgcatgatca atgcctccgg ctacaaggtg
    15721 tggccggccg aggtcgaggc gctgctctac gcgcaccccg acatccagga agcctgcgtc
    15781 atcggcacgc ccgacggcta tcgcggcgag agcgtgaaag cggtgatcgt cctcaaggaa
    15841 gcatccagga aaagattgtc tcccgaagac atcagcgaat gggcgcgcgg ccgcatggca
    15901 gccttcaagg tgccgcgcgt ggtcgagttc gtcgagcagc tgccgaaaac ccccaccgga
    15961 aagatcctgt ggcgcgagct gcaggagaaa gagaggcgta aatgaggcat gccctttttg
    16021 tgctttgcat cgtcgccacg ccggttctcg gtcaggtgaa catcgagaac gcctgggcgc
    16081 gcgccacggc gccgggcgcg aagctgggcg caggctacat gacgatccgc aacgcgtccg
    16141 gcgagcctga ccgcctagtc gcggcctcgt cgccgcgcgc gcagcgggtc gagatgcatg
    16201 tgcatctgaa ggatggcgag gtgatgaaga tgcgcgaggt gaagggcttc gacatcccgc
    16261 cgcgcggcgg cttcgagctc aagccgggcg gcgcgcacct catgttcgtc agcatcggtg
    16321 cgcccttcaa ggagggcgag aaaatcccgg tgaagctgcg cttccagcgc gcgggcgaag
    16381 tgaagaccga gttccaggtc ggccggctcg ccgacgccgg gccgccgcac ggcaagcact
    16441 agcccgcgaa cagcgcgcgc gcggccgccc aggcaagcgc ggcgatgaag gccgagcagg
    16501 ggatggtgag aatccaggcc cagacgatat tgcccgcgat tccccagcgc accgccgaga
    16561 cgctctgtgc cgcacctacg ccgataatgg cgccggtaat ggtgtgagtt gttgacactg
    16621 ggatgcccag aaaacccgtg gtgaagagcg taatcgcgcc gccggtctcg gcgcagaagc
    16681 cgccgaccgg cctgagcttc gtgatcttct ggcccatggt cttgacgatg cgccagcctc
    16741 cgaacagggt ccccagcgcg atcgtgatgt agcaggccac cgcgacccac aatggaaatg
    16801 gatctttggc gccgtaatac ccagcggcga tcaggagcat ccagatgatg ccggcagttt
    16861 tctgcgcatc gttgccgccg tgtccgaggc tgtagaaaga agccgacacg agctgcatgc
    16921 gccggaacca gcgatcgacc ttggagggcg cagaccgcct gaacagccac gcaaccgcga
    16981 gcatcatcat ggagctcagg gcaaacccga ggagcggcga caggatgatg aagagaaata
    17041 tcttggacac tccggccggg actagtatcc acatgccgcc tttcgccacg cccgcgccga
    17101 tgatgccgcc gatcagcgcg tgcgaggatg aagaggggat gccgtagtac caggtgatga
    17161 tgtcccaggc gatggccc
//
LOCUS       DASPXW010000021         2395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1519864, whole genome shotgun sequence.
ACCESSION   DASPXW010000021 DASPXW010000000
VERSION     DASPXW010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1519864"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(537..1301)
                     /locus_tag="VEL04_00505"
     CDS             complement(537..1301)
                     /locus_tag="VEL04_00505"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYT46875.1"
                     /translation="MERAPSHQLAEHLGVWHRKPVLRLVYEDFYDRIAVACLSGRTIE
                     IGGGIGNLKRRLSEVVATDIQSAPWLDCVADAQRLPFADACAANIVMVDVLHHLEFPL
                     VFFREAARVLRPGGRLLMVEPAITWGSTVFYRLFHHEPVRTAADALEDGSPDPRRDPY
                     DSNQAIPTLIATRDRDRFHRLVPALRIAGVQWFALAAYPLSGGFQRWSLIGEGVARRL
                     LRIERALEPALGRLTAFRMLLTVEKAAGGTAARYVE"
     gene            complement(1301..>2395)
                     /locus_tag="VEL04_00510"
     CDS             complement(1301..>2395)
                     /locus_tag="VEL04_00510"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYT46876.1"
                     /translation="VVVDTLGALDDCQAMFANLHYLCTRETRVVVGYFSHLWYPALKL
                     AEATGMKMPQPPQNVLSRADVRALAALGDFDAVKDETRVLSPVRLFGLGRLVNRFLAP
                     LPLIGSLCLRHFTVCRSLRRSDEVRSATIVIPARNERGNIEAAVKRIPRFAETLEIIF
                     VEGHSRDGTWEEIERVAAAYPDCNIKAMRQPGTGKGDAVFAGFDAARGDVLMILDADL
                     TMPPEQLPKFWDAIRSGKGEFVNGSRLIYPMEQEAMRFLNLVANRAFSLLFTWLLSQR
                     FTDTLCGTKAMRRSDYARIKAARSYFGEFDPFGDFELIFGASKLGLKAVEIPIRYASR
                     TYGETQISRFRHGFMLLRMVLFAFMRIKAM"
BASE COUNT          444 a          780 c          815 g          356 t
ORIGIN      
        1 atagaacgcg cagccgcgct ttcggtttcc accctcgatt tgccagtctc agaagaagcg
       61 tgggggtggc aaaggcgcgt cttcccggca tgtcgatttc acgcgtgggc acgcttcgct
      121 ttgcccaccc ctactcgctg gaacctaaag ccctcctcac gacttaacac aatcgagcgt
      181 tcggcgagtt cgctctcgag gctcccgccg ggggagctcg agagcgcggc agctccaaag
      241 ggggaaccgt catgtggcaa aagaccggat attgccggca gaaggcggcg gagtgcgcgg
      301 cacgcgcgca agaagccgcc gacaaggaca cgcgcgcgct ttttctcaaa tttcgggatt
      361 catggctcag cgcggcgcac cgatacgaat ccctgcccgc gaccgccccc ggcgagacgg
      421 cggaccgcca gccgtcgttc gggcccgagc ccggcgctcg cgaatggcgt tggcgcccga
      481 gcgacgcgcg aatggatacg acggcggagc ggctcggagc ctgctgatcc tccgcgtcac
      541 tcaacgtaac gcgcggccgt tccgcctgcc gccttctcga ccgtcagcag catgcgaaac
      601 gccgtgaggc gaccgagcgc cggctcgagc gcgcgctcga tgcgcagcag ccggcgcgca
      661 acgccttcgc cgatgaggct ccaacgctgg aagccgccgc tcagcggata ggccgcgagc
      721 gcgaaccatt gcacgcccgc gatgcgcagc gcgggaacga ggcgatggaa gcggtcgcga
      781 tcacgggtgg cgatgagcgt cgggatggcc tggttggagt cgtaggggtc gcggcgcgga
      841 tcaggactgc cgtcctcgag cgcgtcggcc gcggtgcgca cgggctcgtg atgaaagagc
      901 cggtagaaca ccgtgctgcc ccaggtgatc gccggctcga ccatgagcag gcgcccgccc
      961 ggccgcagca cccgcgccgc ctcgcggaag aagacgagcg gaaattcgag atgatgcagc
     1021 acgtcgacca tgacgatatt ggcggcgcag gcgtccgcga acggcagccg ctgggcgtcc
     1081 gcgacgcagt cgagccacgg tgcggattgg atatcggttg cgaccacctc gctcagccgc
     1141 cgcttgaggt tgccgatacc gccgccgatt tcgatggtgc ggccgctcag gcaggcgacg
     1201 gcgatgcgat cgtagaaatc ctcgtagacg aggcgcagga ccggcttgcg atgccacacg
     1261 ccgaggtgct ccgcgagctg atgggatggc gcgcgctcca tcacatcgcc ttgatgcgca
     1321 tgaacgcgaa cagcaccatg cgcagcagca tgaagccgtg acggaaccgg gagatctggg
     1381 tctcgccgta ggtgcggctg gcatagcgga tcggaatctc gaccgccttg aggccgagct
     1441 tcgacgcgcc gaagatcagc tcgaaatccc cgaacggatc gaactccccg aaatagctgc
     1501 gcgcggcctt gatccgtgca tagtcggagc gccgcatcgc cttggtgccg cacagcgtgt
     1561 cggtgaagcg ctgcgacaac agccaggtga aaagcaacga gaaggccctg ttggcgacga
     1621 gattgaggaa gcgcatcgcc tcctgctcca tcggatatat gagccgggag ccgttgacga
     1681 actcgccctt gccggagcgg atcgcgtccc aaaacttcgg caactgctcc ggcggcatgg
     1741 tgagatcggc gtcgaggatc atcaggacgt cgccgcgcgc ggcatcgaag ccggcgaaca
     1801 cggcatcgcc cttgcccgtc cccggctggc gcatcgcctt gatgttgcaa tcgggataag
     1861 cggcggcgac gcgctcgatc tcctcccagg tgccgtcccg ggagtggccc tcgacgaaga
     1921 tgatttcgag cgtctccgcg aagcggggga tgcgcttgac cgccgcctcg atgttgccgc
     1981 gctcgttgcg cgcgggaatg acgatcgtcg cggatctcac ctcgtcggat cgccgcagcg
     2041 agcggcaaac ggtgaaatgg cgcaagcaca ggctgccgat gagcggcaac ggcgccagga
     2101 agcggttgac caggcggccg agcccgaaca gccgcaccgg agagagcacg cgcgtttcgt
     2161 ccttgaccgc gtcgaagtcg ccgagcgccg cgagcgcgcg cacgtcagcg cgagagagga
     2221 cgttttgcgg aggctgcggc attttcatgc ccgtagcctc ggccaatttc agcgccggat
     2281 accacaagtg cgaaaagtag ccgaccacga cgcgggtctc gcgcgtgcac aaatagtgca
     2341 ggttggcgaa catcgcctgg caatcgtcga gcgcgccgag cgtgtcgacc accac
//
LOCUS       DASPXW010000022         2201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1772611, whole genome shotgun sequence.
ACCESSION   DASPXW010000022 DASPXW010000000
VERSION     DASPXW010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1772611"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..618)
                     /locus_tag="VEL04_00515"
     CDS             complement(124..618)
                     /locus_tag="VEL04_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HYT46877.1"
                     /translation="MKAALESVEISLTVNGERVRRSVPARTNLADFLRNELELTGTHV
                     GCEHGVCGACTVRMDGEIVRGCLMFAAQADGCKVDTIEGLSDSRELEELQSAFVLTNA
                     LQCGFCTPGVLMAAQALLRDQRNPSRERIREYLAGNYCRCTGYQAIVDAIEAVAGARA
                     ARSK"
     gene            complement(615..1478)
                     /locus_tag="VEL04_00520"
     CDS             complement(615..1478)
                     /locus_tag="VEL04_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HYT46878.1"
                     /translation="MKAPRFAYARPASIAEALALLAEHKDDARVLAGGQSLVPMLNFR
                     VAAPKVLVDINRIVGLVGVKVAGNHIRIGALTRHVELERSTDIARHLPLIAAAMPHIA
                     HPAVRNRGTFGGSCALADPAAELPPCALALGATFIIASKKGERRVAAADFFKGLYATA
                     LKADELLIAAEFPLPKPGYACAFGELARRHGDYAMVGVAAHGSTDGKKFSDMRVALFG
                     VGDRPVRAARLERALDGRPAAAKTIEGALAALDSDLEPRADLQASAATKRHLAKVLAG
                     RVLRNMQGVAA"
     gene            1525..2094
                     /locus_tag="VEL04_00525"
     CDS             1525..2094
                     /locus_tag="VEL04_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYT46879.1"
                     /translation="MNYRETLPRRGISHKEMMKRVARFRKLKGSDGGLPDSKMPGCER
                     ILYNVIGFQPPEVEANGKQSPVGLKAARMAAIKISEGFNLGYCRARPGKGPMLHNHDT
                     NETFIAMSGTWRAIWEGKGGKLEHVDLKPLDVISFPPGAIRRFMNVTRGPKNKYSTLM
                     FVIGGNAPSSEFTGRSMREIEAAGALPRK"
BASE COUNT          358 a          727 c          754 g          362 t
ORIGIN      
        1 cgcttgtctt cgtcgatgtc cagtgcggtc aggatgactc gcgtcccggc gatggcgtgc
       61 aggcgcaggg cgccttgctg attgcttgct cgcattgcac ctccgaagtg ttggttgtcg
      121 gtgttacttc gatctcgccg cgcgcgcccc ggcgaccgcc tcgatcgcat cgacgatcgc
      181 ctggtatccg gtgcagcggc agtaattgcc cgcaaggtac tcgcggatgc gctcgcgcga
      241 aggattccgc tggtcgcgca ggagcgcctg cgcggccatc agcacgcccg gcgtgcagaa
      301 gccgcattgc agcgcgttgg tgagcacgaa ggcgctctgc agctcctcga gctcgcgcga
      361 gtccgacagg ccctcgatcg tgtccacctt gcagccatcg gcctgcgcgg cgaacataag
      421 gcagccgcgc acgatctccc cgtccatgcg caccgtgcag gctccgcaca cgccgtgctc
      481 gcagccgacg tgggtgccgg tgagctcgag ctcgtttctg aggaaatcgg cgaggttcgt
      541 gcgcgcgggc acgctgcgcc tcacgcgctc gccgttcacc gtgagggaga tctccaccga
      601 ctcgagggcg gccttcacgc ggcgaccccc tgcatgttcc tgaggacccg cccggcgagc
      661 accttggcga ggtgccgctt ggtcgcagcc gaggcttgca agtcggcgcg cggttcgagg
      721 tcggagtcga gcgccgcgag cgcgccctcg atcgtcttcg ccgccgcagg tcggccgtcg
      781 agggcgcgct cgagccgcgc tgcgcgaacc ggccggtcgc ccaccccgaa gagcgccacg
      841 cgcatatcgg agaacttttt cccgtcggtc gagccgtgcg ccgcgactcc caccatcgcg
      901 taatcgccgt ggcggcgcgc gagctccccg aaggcgcagg cgtagcccgg tttcggcagc
      961 ggaaactccg cggcgatgag cagctcgtcc gctttgagcg cggtggcgta aaggcccttg
     1021 aaaaaatcag cggccgcgac acgccgctcg ccctttttgc tggcgatgat gaaagtcgcg
     1081 ccgagcgcga gcgcgcaggg gggcaattcc gccgccgggt cggcgagagc gcagctgcca
     1141 ccgaaagtgc cgcggttgcg gacggcggga tgcgcgatat gcggcatcgc ggctgcgatg
     1201 agcggcaggt gccgggcgat atcggtggag cgttccagct cgacgtggcg ggtgagcgcg
     1261 ccgatgcgga tgtggttccc ggcgaccttg acccctacga ggcccacgat gcggttgatg
     1321 tcgacgagga ccttgggcgc cgcgacgcgg aagttcagca tcggcacgag gctctggccg
     1381 ccggcgagca cccgcgcatc gtccttgtgc tcggcgagca gcgcgagcgc ctcggcgatg
     1441 ctcgccggtc tcgcgtaggc aaagcgcggc gctttcatcc cttctcctcg gtagtattat
     1501 gcagcatcgg ttttggaggt tcccatgaat taccgagaga ccctgccgcg ccgcggcatt
     1561 tcgcacaagg aaatgatgaa gcgcgtcgcg cgcttcagga agctcaaagg ctccgacggc
     1621 gggttgcccg attccaagat gcccggctgc gagcgcattc tctacaacgt catcggcttc
     1681 cagccgcccg aagtcgaggc gaacggcaag caatcgccgg tgggactgaa ggcggcgaga
     1741 atggccgcga tcaagatctc cgaaggattc aacctcggct actgccgggc gcgcccgggc
     1801 aaaggtccga tgctgcacaa ccacgacacc aacgagacct tcatcgcgat gagcggaacc
     1861 tggcgcgcga tctgggaggg caagggcggc aagctcgagc acgtggacct gaagccgctc
     1921 gacgtgatct cgttcccgcc cggggcgatc cgccgcttca tgaacgtcac ccgcgggccg
     1981 aagaacaaat actcgaccct gatgttcgtg atcggcggca acgcgccgag ctcggaattc
     2041 accggtcggt cgatgcgcga gatcgaagcc gcgggcgcgc tgccgcgaaa gtgaggcgtt
     2101 tcccctcccg gaggggttca tttttgcccg gaaaccgggt atgtttcgcc tgttgtgctc
     2161 cagtgcccgc ggtccagcgg gaacaaaaaa gggagggagc c
//
LOCUS       DASPXW010000023         9567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1520305, whole genome shotgun sequence.
ACCESSION   DASPXW010000023 DASPXW010000000
VERSION     DASPXW010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9567
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1520305"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="VEL04_00530"
     CDS             complement(<1..821)
                     /locus_tag="VEL04_00530"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HYT46880.1"
                     /translation="MGRPLALFGVPAILCGAWTVFAGKDLNWDLLHYHYYIAHSLLHG
                     RFAQDYFAAGTESYLNPIGYVPFYLMVSAGWHSVLVSVLLAALHGASLALLYVLAWTL
                     FRRLPWRQRATLSALAAAVGAASAVFWATVGTSFLDPLLAVPMLGAVVLLARAQPADA
                     IQRSAWAGVLFGAAAALKYSNAFYALAAMILCVAIPGIAWPRRLRAVAAYAGGGAIAV
                     GLLAGPWLARLAREFGNPFFPHLNALFRSPEAPPFMLLAERFAPQDLAGALAFPFR"
     gene            complement(832..1980)
                     /locus_tag="VEL04_00535"
     CDS             complement(832..1980)
                     /locus_tag="VEL04_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYT46881.1"
                     /translation="MPELTILMPCLDEAATVGACVTKAHGFLQRAGIDGEVLVADNGS
                     SDGSAEIAAAHGARVISVADRGYGAALRAGVRAAKGRYVIMGDADDSYDFSRLEGFVE
                     KLRAGHPLVMGNRFKGAILPGAMPALHRYLGNPVLSFLGRLFFRTRIGDFHCGLRGFE
                     RDAVLGLGLRTRGMEFASEMVVKAVLARWHIAEVPATLAPDGRGRPAHLRSWHDGWRH
                     LRFLLLFSPRWLFLYPGCTLLVSGLALTVALYFAPLRIGGAGLDIHTMLYAAAAALLG
                     LQLCLFALFARVSAQAAGLLPRQPSLDRLLRVITLERGLLAGLGVALTGLIWSAAAFW
                     QWRDAGFGALDPRAMMRETIPASALMIGGMEMALASFLLSVLRLNESQ"
     gene            complement(1996..3648)
                     /locus_tag="VEL04_00540"
     CDS             complement(1996..3648)
                     /locus_tag="VEL04_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYT46882.1"
                     /translation="MAALGLLAAMACALYLPYLGNPAVFDDRLFFSGLGFSRYASAPF
                     GLGVRVPAYFSLAIVEVVFGRIEAHRVVSLVLHIACAWAVYALIRALSVRQACAFAGA
                     ALFAVHPVAVYGAGYLVQRSILLATLFGLLSLLLYCRGLRHGSYSDALAAAVLYSLAV
                     LSKEHAVLLPAVAAALVPLAAQTRRFAMRYTALYFLACAPAAVLVVLLSTGVIGQAYE
                     PYFDVVAAQVTNAYWSETLSSPWVGSVIAQAALFFKYAALWLVPITAGMSLDVRVDFT
                     ELWSPAIALPALFGFLAFGALAAYLLTKRVRVGIAAFGLWYVWILFFTEFATIRFQEP
                     FVLYRSYLWAPGLAIAVAAALECLQPRVMLALLMPALAVLSWQANDRLQTFSSGLAVW
                     EDAAAKLPPGAIPGGHRPLYEAGREYLYAGRVQDALQATERCMKLYPTTYDCFLARGA
                     LHIHLGQNETALPYVMKAIALRPGDGVARHHLGLILENLGCLDEAKAHYRDAVERHFP
                     GAQHRLDRIESPGKGLLPPRQAPPPRGDCSKLLVRNRAFRPG"
     gene            complement(3742..4221)
                     /locus_tag="VEL04_00545"
     CDS             complement(3742..4221)
                     /locus_tag="VEL04_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HYT46883.1"
                     /translation="MKRIQQGFTLIELMIVVAIIGILAAVALPAYQDYTVRAKMSEVV
                     LAASSCRTTISEIWQSGGSSPAANSWGCGEGGGTTTKYVGSINTSADGWITVTAVNLG
                     AANGVVSMVPMKNGTTALLSTDMSAGVAIFGWRCGNSVTDGTTVSAKYLPGTCRGTF"
     gene            complement(4425..5987)
                     /locus_tag="VEL04_00550"
     CDS             complement(4425..5987)
                     /locus_tag="VEL04_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HYT46884.1"
                     /translation="MQRLEQLNSIGASLSAERDINRLLEAILAAAKTITRADGGSLYR
                     LTEERTLRFEIVRTSSLEYYLGGTSGNPVPFYPIQLYKDGKPNHSMVAAYAALTGKTV
                     NIADAYTAEGFDFSGTRAFDSKTGYRSKSFLTVPMRNHENEAIGVLQLINAQDPRSGE
                     IVAFSASDQRLAESLASQAAIALTNRMLINQLEQLFESFINMINSAIDEKSPYTGGHC
                     QRVPVLTMLLAEAVNETKAGPLSNFNMNEKDRYELKIAGLLHDCGKVTTPVHVVDKAT
                     KLEAIYDRIHLIDTRFEVLKRDLELESLKKKIGGKQLRDRLRELEDDCRFLHACNIGG
                     ERMRDEDVERVRRIGQYRWRDISGHEANFLTDDEVKNLTIRAGTLTPEERQIINHHIV
                     ATIKMLEALPWPKHLAKVAEYAGGHHERMDGKGYPRGLTREQMSVQARCMGIADIFEA
                     LTAKDRPYKKGKTLSESLEILGRMKLNHHVDPDLFDIFVRRKVYRRYAEMFLDPEQID
                     AVDEARIPGYQP"
     gene            complement(6079..8454)
                     /locus_tag="VEL04_00555"
     CDS             complement(6079..8454)
                     /locus_tag="VEL04_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HYT46885.1"
                     /translation="MSAVLSHQAQTQTDVGARLAFQKQLQAVTNKIHATNNIDEIMLE
                     VSADVCALFNADRLTIYSVSEDKGSIVSKVKTGLNSFKDLKLPIAEHSIAGYTALAKK
                     TINIKDCYDDSELKGINPNLRFLQEVDKRTGYRTKQQLVAPIVEAGSSELIGVIQIIN
                     NRSGAPFGALAEEGVAELSQTLSIAFKQRQKPQLVKTKYDYLIADAVLSAGEFELASR
                     QARKKAIDVEQVLTDEFQVKIPAIGQALSKFFGVPYEVFKQDRIKPADLLKNLKREYV
                     ESNQWLPIDDTKEGLVVLCLDPERIRSSRIASNVFPKSRLVYKVTTQKDFRETVNQFY
                     GQEAAMDLSSVDELLSGMDDDAGGEALEGSGADEVSAAADNELVKLVNKVIVDAYNQG
                     ASDIHIEPYPGKAKTEVRFRKDGSLGPYIEVPATYRAAIVARIKIMCDLDISEKRKPQ
                     DGKIKFKKFGPLDIELRVATIPTAGGVEDIVMRILAAGEPIPLEKMGFTTRNLDELHK
                     TVSKPYGLFFVCGPTGSGKTTTLHSVLKYLNTPDTKIWTAEDPVEITQKGLRQVQVNK
                     KAGLDFAVVMKAFLRADPDIIMVGEMRDKETTSTGIEASLTGHLVLATLHTNSATESV
                     IRLLDMGMDPFNFADALLGILAQRLAKRICSCKQPYTPEAAELTSFLREYCEELMNTS
                     RFKADPKGSMEAVYKDWVKTFGNDKGQLTFYKPVGCDKCGGTGFKGRCGLHELLIATD
                     KLKKLVQEHSRVAEMLAQCLEDGMRTLKQDGMEKCLMGVTHMKEVRAVCIK"
     gene            complement(8551..>9567)
                     /locus_tag="VEL04_00560"
     CDS             complement(8551..>9567)
                     /locus_tag="VEL04_00560"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HYT46886.1"
                     /translation="AGLSRERLAELADVVAVERAPRGADPLKERSHAVQSLFLLAGEL
                     LLVFHGGGTLLIVGSTEEARQALNRRKPAVVRTKAITDVDLLAVDDDALDILATWDQV
                     AAGSGDGAAAMARAVHSDSRLAGGAFSLGSLRNGAFARLPAAHIDELLKRFERVRAAR
                     GDVVIREGDEGDHYYVVESGRFQVERLVGGARMVLAELKSGDAFGEEALVSEAKRNAT
                     VTALSAGEVLRLGRSHFNELLREPLLRRVEFTQAAQKVQRGALWLDVRYPSEYQYDKL
                     PDAINVPLAEVRNMFAVLDRSREYIAYCQSGRRSAAAAFLFSQRGFKVWLLDGGLKAT
                     QKAG"
BASE COUNT         1664 a         3116 c         3163 g         1624 t
ORIGIN      
        1 cggaacggga acgccaatgc gccggcgagg tcctgcggcg cgaagcgctc cgcgagcagc
       61 atgaacggcg gcgcctcggg cgagcggaac agcgcattga ggtgcggaaa gaacggattg
      121 ccgaattcgc gcgcaaggcg cgccagccac ggcccggcga gaaggccgac cgcgatggcg
      181 cctccgcctg cataggccgc gacggctcgc aggcgccgag gccaggcgat gcccgggatt
      241 gcgacgcaaa ggatcatcgc cgcgagcgca tagaaggcgt tggagtactt gagcgctgcc
      301 gccgcgccga agagcacgcc ggcccatgcc gagcgctgga tggcgtcggc cggctgcgcg
      361 cgagcgagca gcaccactgc gcccagcatc ggcaccgcca gcaacggatc gaggaacgac
      421 gtgccgacgg tggcccagaa caccgcgctc gccgctccaa cggcggcggc aagcgcggac
      481 agcgtggcac gctggcgcca cggaaggcgc cggaacagcg tccaggcgag tacgtagagc
      541 agcgcgaggc tagcgccgtg cagggccgca agcagcacgg acaccagaac gctgtgccaa
      601 ccggccgaaa ccatgagata gaacggcaca tagccgatcg ggttgagata gctctccgtg
      661 cccgcggcaa agtagtcctg cgcgaagcgc ccgtgcagaa gcgagtgggc tatgtaatag
      721 tggtaatgga gcaggtccca gttgagatcc ttcccggcga acaccgtcca cgctccgcac
      781 aggattgccg ggactccgaa cagcgccagc ggtctgccca tcgaagcggg gctactggct
      841 ttcgttcagc cgcagcacgc tgagcaggaa cgaggcgagc gccatttcca tgccgccgat
      901 catcagcgcg ctcgccggaa tggtctcgcg catcatggcg cgcggatcga gcgcgccgaa
      961 tcccgcgtcg cgccactgcc agaaggccgc cgcgctccag atcaggccgg tcagcgcgac
     1021 gccgagcccg gcaagcaggc cgcgctccag cgtgatcacg cgcagcagcc tgtcgagtga
     1081 cggctgccgg ggcaggagcc cggctgcctg ggccgaaacg cgcgcgaaca gcgcgaacag
     1141 gcacagctgc agcccgagca atgctgcggc agccgcatac agcatggtgt ggatatcgag
     1201 cccggcgccg ccgatgcgca gcggcgcaaa atagagcgcc acggtcagcg caagcccgga
     1261 caccagcagc gtgcagccgg gatacaggaa cagccatcgc gggctgaaga gcagcaggaa
     1321 gcgcaggtga cgccagccgt cgtgccagct cctcaggtgc gccggccggc cccggccgtc
     1381 cggcgcaagc gtggcgggaa cttccgcgat gtgccatcgg gcaagcaccg ccttgaccac
     1441 catctcgctc gcgaattcca tgccgcgagt ccgcagcccc aggccaagca cggcgtcacg
     1501 ctcgaaaccg cgcagcccgc agtggaaatc cccgatccgc gtgcggaaga acagccgtcc
     1561 caggaagctc agcaccgggt tgccgagata gcggtgcagc gccggcattg cgccgggaag
     1621 aatggcgcct ttgaagcgat tacccatcac cagaggatgg cccgcgcgca gcttttccac
     1681 gaagccttcg agccggctga agtcgtagct gtcatcggcg tcgcccatga tcacatagcg
     1741 gcctttcgct gcccgaactc cggcgcgcag cgccgcgccg tagccgcgat cggcgacgct
     1801 gatgacgcgc gcgccatggg ctgcggcgat ctcggcagag ccgtcgctcg agccgttgtc
     1861 ggcaaccagc acttcgccgt cgattccggc gcgctgcaga aaaccgtgcg ccttcgtgac
     1921 gcaagcgccg acggtcgccg cctcgtcgag gcaaggcatg agaatggtca attcaggcat
     1981 tcgcggagac gcggctcagc ccggccgaaa tgccctgttt cgcacgagca gcttgctgca
     2041 atcgcccctc ggcggcggcg cctgacgcgg cggtaacaac ccctttccgg gggattcgat
     2101 gcggtccaac cggtgctgtg cgcctgggaa atgccgctcc accgcatcac gataatgtgc
     2161 cttggcctcg tccaagcacc cgaggttctc caggatcaat cccagatgat gccgcgccac
     2221 gccgtcccca gggcgcagcg cgatcgcctt catgacatag ggcaaggccg tttcgttctg
     2281 ccccagatga atatggagcg cgccgcgggc gaggaagcag tcgtaggtgg tcggatagag
     2341 tttcatgcag cgctcagtgg cctggagcgc gtcctggacc cgcccggcgt acaagtactc
     2401 gcggcccgcc tcgtagagcg ggcggtggcc gccgggaatc gcgccgggcg gtagtttagc
     2461 cgccgcgtcc tcccataccg ccaggccact gctaaaggtt tgcaggcggt cgtttgcctg
     2521 ccacgacaac acggcgagcg ccggcatgag caatgccagc atcacgcggg gctgcaggca
     2581 ctcgagcgcc gccgccacgg cgatagcgag acccggggcc cagagatagc tgcggtacag
     2641 gacgaacggc tcctggaagc ggatcgtcgc gaattccgtg aagaacagga tccagacgta
     2701 ccagaggcca aatgccgcga tgccaaccct gacccgcttg gtgagcaggt aggcggccaa
     2761 cgccccaaag gcaaggaagc cgaaaagcgc gggaagcgcg atggcgggcg accacagctc
     2821 ggtaaagtcg acgcgcacgt caagcgacat gccggccgta atgggcacga gccatagcgc
     2881 cgcgtatttg aagaacagcg ctgcctgcgc gatgacgctg cccacccatg gcgaggacag
     2941 cgtctcgctc cagtaggcgt tcgtcacctg cgccgcaacg acgtcaaagt acggctcata
     3001 agcctgcccg atgacgcctg tgctgagcag cacgacgaga acagcggctg gcgcgcaggc
     3061 caggaagtag agcgccgtgt agcgcatggc gaagcgccgc gtctgggcgg caagcggcac
     3121 gagcgccgcg gccaccgcgg gaagcagcac ggcgtgctcc ttggacaaca ccgccagcga
     3181 atacaggacg gcggcggcga gcgcatcgga gtagctgccg tggcgcaggc cgcggcaata
     3241 gagcaggagc gaaagcagcc cgaacagtgt cgcgagcagg atcgaacgct ggacgaggta
     3301 accagcgccg tagaccgcca ccggatgcac ggcgaacagc gcggcacccg cgaacgcgca
     3361 cgcctgccgg accgacagcg cccggatcag cgcataaacc gcccacgcgc aggcgatatg
     3421 caggaccagg ctgaccacgc gatgcgcttc gatgcgcccg aagaccacct cgacgatggc
     3481 gagcgaaaaa taggccggga ctcggacgcc gaggccgaat ggcgcgctcg catagcgcga
     3541 aaagccgagg cccgagaaga acagcctgtc atcgaacacc gccggattgc cgaggtaggg
     3601 cagatacagc gcgcaggcca tcgcggcgag caggccgagc gccgccaatg cgccaagccg
     3661 tgcggatccg ggcaatgtgg aaaacacagg tcaaaaaaaa aggtaccccg aagggtacct
     3721 tttcccgcaa cgagtctgcg attagaaagt accgcggcag gtgccgggca ggtacttggc
     3781 agaaaccgtc gtgccgtcgg tgaccgagtt gccgcagcgc cagccgaaga tggcgacacc
     3841 ggccgacatg tcggtggaga ggagggcggt ggtgccgttc ttcatcggga ccatggagac
     3901 gacgccgttg gccgcaccaa ggtttaccgc agtcacggtt atccagccgt cagcgctcgt
     3961 attgatggag ccgacgtact tcgtagtcgt gccgccgccc tcgccgcatc cccagctgtt
     4021 ggcagcgggc gagctgccgc cggactgcca gatctcgctg atggtcgtgc ggcaagaact
     4081 cgccgccagg accacctcgg acatcttcgc ccgcacggtg tagtcctggt acgcgggcag
     4141 cgcgaccgcc gccagaatgc cgatgatcgc gaccacgatc atcagttcga tcaacgtgaa
     4201 gccctgctgg atgcgtttca ttagcactcc ctagttgaga atgtggtgga gattcggcca
     4261 aaaccgcttg accgtaccac tcctgcagca aggatcatgc ccgggggttt tgcgcgctca
     4321 ttcgtgagaa attcctcgct cccgggggcg tcttgcgcgg gtaatgtcca cccttggccg
     4381 gggccgccta gcccaaatct ggcagctccg cccaatctcg tcaattacgg ctggtagccc
     4441 ggaatgcgcg cttcgtcgac cgcgtcgatc tgctcggggt cgaggaacat ctcggcatac
     4501 cggcggtaca ccttgcggcg cacgaagatg tcgaacagat ccggatcgac gtggtggttc
     4561 agcttcatgc ggccgaggat ctcgagcgac tcggacagcg tcttgccctt cttgtagggc
     4621 cggtccttgg ctgtgagcgc ctcgaaaatg tcggcgatgc ccatgcagcg cgcctgcacc
     4681 gacatctgct cgcgcgtcag tcctctgggg tagcccttgc cgtccatgcg ctcgtggtga
     4741 ccgccggcgt actcggcaac cttggcgagg tgcttgggcc agggcaaggc ctccagcatc
     4801 ttgatggtgg ccacgatgtg gtgattgatg atctggcgct cttccggggt cagcgtgccg
     4861 gcgcggatgg tgagattctt cacctcgtcg tcggtcagga aattggcctc gtggccggag
     4921 atgtcgcgcc agcggtactg gccgatgcgc cgcacccgct cgacgtcctc gtcgcgcatg
     4981 cgctcgccgc cgatgttgca ggcgtgcagg aacctgcagt cgtcctcgag ctcgcgcagg
     5041 cgatcgcgca gctgtttccc gcctatcttt ttcttcagcg attccagctc gaggtcgcgc
     5101 ttcagcacct cgaagcgcgt gtcgatgagg tggatgcggt cgtagatcgc ctcgagcttg
     5161 gtcgccttgt cgaccacgtg caccggcgtg gtgaccttgc cgcagtcgtg cagcaggccc
     5221 gcgatcttca gctcgtagcg gtccttctcg ttcatgttga aattgcttag cggcccggcc
     5281 ttggtctcgt tcacggcctc ggcgagcagc atcgtcagca ccggcacgcg ctggcaatgg
     5341 ccgccggtgt agggcgattt ctcgtcgatc gcgctattga tcatgttgat gaacgactcg
     5401 aacagctgct cgagctggtt gatcagcatg cggttggtga gcgcgatcgc cgcttgcgag
     5461 gcgagcgatt ccgcgagccg ctgatcggat gcggaaaatg ccacgatctc gccgctgcgc
     5521 gggtcctggg cgttgatcag ctgcagcacg ccgatggcct cgttctcgtg gttgcgcatc
     5581 ggcacggtga ggaacgattt cgagcggtag ccggtcttgg aatcgaaggc gcgggtgccg
     5641 gagaagtcga aaccctcggc tgtgtaggcg tcggcgatat tcactgtttt gcccgtgagg
     5701 gcggcgtacg ccgccaccat gctgtggttc ggcttgccgt ccttgtagag ctggatgggg
     5761 tagaacggca ccgggttgcc ggaagtgccg ccgaggtagt actcgagcga gctggtgcgc
     5821 acgatttcga agcgcagcgt gcgctcctcc gtgagccggt acaggctgcc gccgtcggcg
     5881 cgcgtgatgg tcttcgccgc ggcaaggatc gcctcgagaa ggcgattgat gtcgcgctcg
     5941 gccgagagcg acgcgccgat cgaattgagc tgctcgaggc gctgcatcag gtcctcggcg
     6001 gcgctcggcg ccaagtccgc gaggcgcagg ccgaccgcgc tgtcgatcag gctgctcatg
     6061 cgctcggccg cggcggcgct acttgatgca cacggcccgc acttctttca tgtgggtgac
     6121 ccccatgagg catttctcca tgccgtcctg cttgagggtg cgcatgccgt cctcgaggca
     6181 ctgggcgagc atctccgcga cgcgcgagtg ctcctgcacc agcttcttca gcttgtcggt
     6241 ggcgatcagc agctcgtgca ggccgcagcg gcccttgaag ccggtgccgc cgcacttgtc
     6301 gcagccgacc ggcttgtaga aggtgagctg tcccttgtcg ttgccgaagg tcttcaccca
     6361 gtccttgtag accgcctcca tcgagccttt cgggtcggcc ttgaagcgcg aggtgttcat
     6421 cagctcttcg cagtactccc tgagaaacga cgtcagctcg gccgcctcgg gggtgtacgg
     6481 ctgcttgcag ctgcaaatgc gcttcgccag gcgctgcgcc aggatgccga gcagcgcgtc
     6541 ggcgaagttg aacgggtcca tgcccatatc gagcaggcgg atgaccgatt ccgtcgccga
     6601 gttggtgtgc aacgtggcga gcaccaggtg gccggtaagc gaagcttcga tgccggtgct
     6661 ggtggtttcc ttgtcgcgca tctcgccgac catgatgatg tcggggtcgg cgcgcaggaa
     6721 cgctttcatc accacggcga agtcgagccc cgccttcttg ttgacctgca cctggcgcag
     6781 ccccttctgc gtgatctcga ccggatcttc ggcggtccag atcttggtgt cgggcgtgtt
     6841 caggtacttg agcaccgagt gcagggtggt ggttttcccc gagccggtgg gtccgcagac
     6901 gaagaacagc ccatagggct tcgagacggt cttgtgcagc tcatccagat tgcgcgtcgt
     6961 gaaacccatt ttctccagcg ggatcggctc accggcggcg agaatccgca tcacgatgtc
     7021 ctcgacgccg cccgcggtgg ggatcgtcgc cacccgcagc tcgatgtcga gcgggccgaa
     7081 cttcttgaac ttgatcttgc cgtcctgggg tttccttttc tcggagatgt ccaggtcgca
     7141 catgatcttg atgcgcgcca cgatcgcggc gcggtaggtc gccggcacct cgatgtaggg
     7201 cccgagcgag ccatccttcc tgaagcgcac ctcggttttc gccttgccgg ggtagggctc
     7261 gatgtgaatg tccgaggcgc cctggttgta ggcatcgacg atgaccttgt tgaccagctt
     7321 caccagctcg ttgtccgccg cggcggagac ttcgtctgcg ccgctgcctt caagcgcctc
     7381 tccgccggca tcgtcgtcca ttccggagag gagctcgtcg acgctcgaca ggtccatggc
     7441 cgcttcctgg ccatagaact ggttgaccgt ttccctgaag tccttctgcg tcgtcacctt
     7501 gtagaccagg cgcgatttcg gaaacacgtt ggaagcgatg cgcgaggagc ggatgcgctc
     7561 gggatcgagg cacagcacca ccagcccttc cttggtgtcg tcgatcggca gccactggtt
     7621 ggattcgacg tactcgcgct tcaggttctt cagcaggtcg gcaggcttga tgcggtcctg
     7681 cttgaacact tcgtagggaa cgccgaagaa tttcgacagc gcctgcccga tcgccgggat
     7741 cttgacctgg aactcgtcgg tcagcacctg ctcgacgtcg atcgccttct tgcgcgcctg
     7801 ccgcgacgcc agctcgaact cgcccgcgga gagcaccgcg tcggcgatca ggtagtcgta
     7861 cttggtcttg acgagttgcg gcttctggcg ctgcttgaac gcgatcgaga gggtctgcga
     7921 aagctcggcg acgccttcct cggcaagcgc gccgaacggc gcgccggaac gattgttgat
     7981 gatctggatg acgccgatca gctcgctgct gccggcctcg acgatcggcg ccaccagctg
     8041 ctgcttggtg cggtagccgg tgcgcttgtc cacttcctgc aggaagcgca agttcgggtt
     8101 gatccccttc agctcgctgt cgtcgtagca gtccttgatg ttgatggtct tcttcgccag
     8161 agcggtgtaa cccgcgatcg agtgctcggc gatcggcagc ttcaggtcct tgaaggagtt
     8221 gaggccggtc ttgaccttgg agacgatcga ccccttgtcc tcgctcaccg agtagatggt
     8281 gaggcggtcg gcgttgaaca gcgcgcacac gtccgcgctc acctcgagca tgatctcgtc
     8341 gatgttgttg gtggcgtgga tcttgttggt gaccgcctgg agttgcttct ggaacgccag
     8401 gcgggcgccg acgtcggtct gcgtctgcgc ctgatggctc aggactgcgc tcattgcctc
     8461 cctccgtgaa tcggaatctg cttctgcgtt tcttgctgga ctttacatcc tagctgtagc
     8521 cgccctgatt ttcaacgaaa ttgtgcctat tcagccggct ttctgcgtgg ctttcagccc
     8581 cccgtccagc agccagacct tgaagccgcg ctgggagaac aggaacgccg cggcggcgct
     8641 gcgccggccg ctctggcaat aggcaatgta ctcccgggaa cggtcgagca ccgcgaacat
     8701 gttgcgcacc tcggcaagcg gcacgttgat cgcgtccggc agcttgtcgt actggtattc
     8761 cgagggataa cgcacatcga gccagagcgc gccgcgctgc actttttgcg cggcctgtgt
     8821 aaattcgacc cgccgcaaca gcggctcgcg cagcagctcg ttgaaatggc tgcggccgag
     8881 gcgcagcacc tcccccgcgc tgagcgcggt gacggtggca ttgcgcttcg cctcggatac
     8941 cagcgcctcc tcgccgaacg catcaccgct cttcagctcg gcgagcacca tcctcgcgcc
     9001 gccgaccagg cgctcgacct ggaagcggcc cgattcgacc acgtagtagt ggtcgccttc
     9061 gtcgccctcg cgaatcacta cgtcgccgcg tgccgcacgc acgcgctcga agcgcttcag
     9121 cagctcgtcg atgtgcgccg ccggcagccg ggcgaacgcc ccgttgcgca ggctgccgag
     9181 cgaaaacgcc ccgcccgcga ggcgcgagtc gctgtgcacc gcgcgcgcca tcgccgcagc
     9241 gccatcgcca ctgcccgcgg cgacctgatc ccaggtcgcg agaatgtcga gcgcatcgtc
     9301 gtcgaccgcc agcaggtcga catcggtgat ggccttggtc cggaccacgg ccggcttgcg
     9361 ccggttgagc gcctggcgcg cctcctcggt gctgccgacg atgagcagcg tgccgccgcc
     9421 gtgaaaaacg agcagcagct cgccggcgag cagaaacagg gactgcacgg cgtgcgagcg
     9481 ctccttgagc gggtcagcgc cgcgcggcgc gcgctcgacc gcgaccacgt cggccagctc
     9541 ggcgaggcgc tcgcgcgaca gcccggc
//
LOCUS       DASPXW010000024         7175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_764175, whole genome shotgun sequence.
ACCESSION   DASPXW010000024 DASPXW010000000
VERSION     DASPXW010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7175
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764175"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /locus_tag="VEL04_00565"
     CDS             <1..1155
                     /locus_tag="VEL04_00565"
                     /inference="COORDINATES: protein motif:HMM:NF014961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HYT46887.1"
                     /translation="GPVIYVPDASRSVPVVQGLLSTESREAFVAQVRADYEKLRAVHR
                     DKKGPGPLHPLQEARRRGLKIAWHAYRPPAPRKPGITVLQRYPLAELVRYIDWTPFFQ
                     AWELSGPYPKILEDPVVGEPARKLFAEAQETLTRIVTENWVKANGVFGLFPAAQVNCD
                     DIEICRDVSGREPLMTWHNLRQQNHKPTGRDNLCLADFVAPRESGARDWIGAFAVSAG
                     GIEQRVAELEAKNDDYGAIMLKVLADRLAEAFAEHLHERVRREFWGYAADEQLSSAEL
                     IAEAYRGIRPAPGYPACPDHRPKGPLFRLLDAERNAAMRLTESFAMLPSASVSGFYLS
                     HPQSSYFAVGKIGRDQIEDYARRAGLTTAEAEKWLAPYLAYEPETTAAPL"
     gene            1164..1265
                     /locus_tag="VEL04_00570"
     CDS             1164..1265
                     /locus_tag="VEL04_00570"
                     /inference="COORDINATES: protein motif:HMM:NF028380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HYT46888.1"
                     /translation="MGKGDKRTRRGKIYRGSYGKKRPQRAKKTKKTA"
     gene            complement(1266..1463)
                     /locus_tag="VEL04_00575"
                     /pseudo
     CDS             complement(1266..1463)
                     /locus_tag="VEL04_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006759755.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(1719..2513)
                     /locus_tag="VEL04_00580"
                     /pseudo
     CDS             complement(1719..2513)
                     /locus_tag="VEL04_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(2518..4593)
                     /locus_tag="VEL04_00585"
     CDS             complement(2518..4593)
                     /locus_tag="VEL04_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYT46889.1"
                     /translation="MKLLLIIGGIVAAAGLFLLATASADVTLFARHYPLALGLNTALA
                     GLLFAIVVYQLIALARRHRARVFGARLTLRLFVRFALLTFVPGLIVYGVSVLFITRSI
                     ESSFSVKVDAALEGGISLGQQAIDQMILDIQSKARAAAIELADRPSAQLAVQLERARE
                     QLAVEEAVIVAANGRLVAGASADVTKFVPELPGAQALRQARIGRGYTSVDAVAGRPLS
                     LRVIVPINSRSFADEARFLQLRQSVPPSFSNSAEAVEAAYRDYKELAISRDGLKRIYI
                     LTLTLSLLMALFVAVSVAVTQSNLLAEPLAHLAQAMQAVARGNFSRQAPVTSRDELGI
                     LTESFNSMTRQLDEARHVVEANRVALEAAKARLESILANLSAGVLVFDHELKLSISNN
                     GAAAILADELASFAEGMREHFVEHGDKDWQFELQLKGTGKMLHARGARLPKETGGGYV
                     LVFDDITQLIQAQRATAWAEVARRLAHEIKNPLTPIQLSAERLEMKLADRLPHEEADT
                     LRRSTQTIVNQVAALKAMVDDFTEYARLPAPELAQLDLNALVGEVLGLYENSIAPIIT
                     RLAPGLPPVWADGAQIRQVIHNLVQNAQDSLDHTRSASANGAIEVRTELAGDRVRLAV
                     SDNGGGFPEELMARIFEPYVTTKPRGTGLGLAIVKKIVDEHHGSVAIENRPSRGASVS
                     VLLPLSKVA"
     gene            complement(4590..5213)
                     /locus_tag="VEL04_00590"
     CDS             complement(4590..5213)
                     /locus_tag="VEL04_00590"
                     /inference="COORDINATES: protein motif:HMM:NF025692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HYT46890.1"
                     /translation="MRRIALLTGFLALLGTAAGPARADTIEVRDAALRAVEDGLVLDA
                     DFAFELTPRLAEVVANGVPLYFRVEFELTRRRWYWFDERSASSLMQMRLSYHALSRHY
                     RLSTGLQQQNLLQQNFATLEEALNVLKRVRNWLVVDRTVAFSRSDYEAAVRMRLDTTL
                     LPKPFQLSALTSRELHLESPWKRFVVRSPQQLPAPVESREPKRPEER"
     gene            complement(5223..6497)
                     /gene="rsmB"
                     /locus_tag="VEL04_00595"
     CDS             complement(5223..6497)
                     /gene="rsmB"
                     /locus_tag="VEL04_00595"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF008149.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HYT46891.1"
                     /translation="MTTGALAQALAKAATVVARVAAGASLSGQIGRLDDDGSATPRAA
                     LIDLTHGTLRRYGRVHAIVRELSHRGRPDALVEALLWCALYALESGRYAEYTVVDHAV
                     RACALLEKWTAKGYVNALLRSAARERASLESRIGADLEARFQHPRWWAETLRSAYPEC
                     WGNVLLAGNSHPPMCLRVNRRRVGTADYQARLAASGISARRVAEHALLLERPVPVERL
                     PGFDAGEVSVQDAGAQRAAPCLDLVSGQHVLDACAAPGGKTSHILETADVEVTAIDSD
                     DERRGRMGRNLDRLRLHATLMAADCTHLAGWWNGERFDRILADVPCSASGIARRRPDL
                     KWLRRAQDIPAFAARQASILDALWQVLGPDGKLLYVTCSVFPQENDAVIDAFVMRTPD
                     ALRLPLPDGAAAQWLPDAEHDGFYYALIQKQA"
     gene            complement(6494..>7175)
                     /gene="fmt"
                     /locus_tag="VEL04_00600"
     CDS             complement(6494..>7175)
                     /gene="fmt"
                     /locus_tag="VEL04_00600"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HYT46892.1"
                     /translation="ALAVAAYGLLLPPQVLELAPFGAWNIHASLLPRWRGAAPIQRAL
                     LAGDRETGISIMRMNAGLDTGPVLAQHRLPIAADDDAGTLHDKLAALGAQAIVAALAA
                     LHAGHAHAVEQSEAGATYARKIDRKDTILNWSLPAAELERAVRAFRPAPGATTLFQGE
                     PIKIWRAGIVSGRDEPGTVLAAGDALVVACRDGALAITELQRAGGRRLAAAHFLRGHA
                     LSPGARLG"
BASE COUNT         1243 a         2392 c         2383 g         1157 t
ORIGIN      
        1 ggtcctgtca tatatgtccc tgacgcctcg cgcagtgtgc cggtggttca ggggctgctt
       61 tcgaccgaaa gtcgcgaagc cttcgttgca caggtacgcg ccgactatga aaagttgcgt
      121 gccgtacacc gggacaagaa agggcccggg ccgctgcatc cgctgcagga agcccgccga
      181 cgcggcttga agatcgcctg gcacgcctac cggccgccgg cgccgcgcaa gccgggcatt
      241 accgtgctgc aacgctatcc gctcgccgag ctggtgcgct acatcgactg gacgccgttc
      301 ttccaggcat gggagctctc gggtccatac ccgaagatcc tggaggatcc ggtcgtcgga
      361 gagcccgccc gcaagctctt cgccgaggcc caagagacgc tcacgcgcat cgtgacggaa
      421 aactgggtca aggcaaacgg cgtcttcggc ctgtttcccg cggcgcaggt caactgcgac
      481 gacatcgaga tctgtcgcga cgtgagcggg cgcgaaccgc tcatgacctg gcataacctg
      541 cggcagcaga accacaagcc aaccgggcgg gacaacctct gccttgccga tttcgttgcc
      601 cctagggaaa gcggcgcccg cgactggatc ggcgcgtttg ctgtcagcgc cggcggcatc
      661 gagcagcgcg tcgcagagct ggaggcgaag aatgacgatt acggcgccat catgctgaag
      721 gtactcgccg accggctggc ggaagccttc gccgagcacc tgcatgagcg cgtgcgacgc
      781 gaattctggg gctatgcagc cgacgagcag ttgagcagcg ccgagctgat tgccgaggcc
      841 tatcgcggca tccggcccgc gcccggctac ccggcatgtc ccgaccacag gccgaagggg
      901 ccgctatttc gtctgctcga cgcggaacgc aacgccgcca tgcggcttac ggagtcattc
      961 gctatgctgc cgtccgcctc ggtatccggg ttttacctgt cgcacccgca gtcgagctat
     1021 ttcgcagtag gcaaaatcgg acgcgaccag atcgaggact acgcgcgccg cgctgggctg
     1081 accactgccg aggcggagaa atggctcgcg ccgtaccttg cgtacgaacc ggagacgacc
     1141 gcggcgccgc tataatccgc ggcatgggta aaggcgacaa acgcactcgg cgcggcaaga
     1201 tctatcgcgg cagctacggc aagaaacggc cacagcgcgc gaaaaaaacc aagaagaccg
     1261 cctgatcagc tctcctcgcg ccggccgacc ggcaggccga gcgccttgag cttgcggtac
     1321 aggtgcgtgc gctcgagtcc cgagcgctcc gcgacgcgcg acatgctgcc gtgctcccgt
     1381 gtcagcaact gctcgaaata cacgcgttca aaggcctcgc gcgcctcgcg gtaagggcgg
     1441 tcgaaagcca cgtcgggcag cgcgccccgc gtcccgcgcg ccgccagcac gcgctcgacg
     1501 tcgtcgagct ggatttcctc ctcgagcgtc gtgagcgcca ggtccttgac cacgctttcg
     1561 agctcggcaa gattgcccgc ccaggcatgg tggcgcaagg cgttcagtgc cgcgatcgcc
     1621 agccgccgcg acgggcaggc acgcgcttcg acgagttggg cgagcatcag cgaagcgaga
     1681 tcgggcacgt cctcggcaaa ctcgcgcagc gccggaagcc tgagcgtcag ctctgccaga
     1741 cgcatcccga gctcggcgtc gaactcgtgc ttttccgaaa gcgtgcgcgc gtccaaggaa
     1801 gagaatgaaa ccacccgcgc cttgtgcttt tcggcgcgcg caagcaggaa ctcaaggttg
     1861 cgctgctcga cccggtcaag acgcgtcagg tccggaatga agaggatgcc gcccgccgcc
     1921 ctggcgagca gctccgaaag cggttcggca agcagctccg cgccggccag gaacggtgca
     1981 cccggcgcgc cgaggagccg tgcgaacagt tccggccgca tgccgcgctc gccgcgcaac
     2041 atgagtggcg cggccgactg ggcgagtagc gcgagccgct tcttggcatc ggcaagcgcc
     2101 ggcgagcgtc cgagggtcgc gagggcaagc tgcggagtcg cagcggtttc cggatgccgc
     2161 agggcccgct tcacggtggc gagcagacgc tgcagggcga tcggcttctc gagaaaatcg
     2221 agcgcgccga tgcgcgtcgc ctcgaccgcg gtctcgatcg tgccgtggcc cgacatcatg
     2281 accaccggca tggtgagctg accgcttgcc gcccattcct tgagcagtgt gatgccatcg
     2341 gtgtccggca tccagatgtc gagcagaacc aggtcgggcc gttgccgctc gcgcaatcgc
     2401 ctcgcgctgc ccgcgttctc cgcgagcatc acctggtggc cttcgtcggc caggatttcc
     2461 gagagcagct cgcggatgcc cacttcgtcg tcaacgacga ggatctgggc catctcgtca
     2521 cgccactttg gatagtggca ggagaacgct caccgaggcc ccgcggctgg ggcgattctc
     2581 gatcgcgacg ctgccatggt gttcgtcgac gatcttcttc acgatcgcca ggccgaggcc
     2641 cgtgccgcgg ggtttggtgg tcacgtacgg ctcgaaaatt cgcgccatca gctcttcggg
     2701 gaatccgccg ccattgtcgg agacggcaag gcgcacgcgg tcgccggcga gctcggtgcg
     2761 cacctcgatt gccccattgg cgctggcgga tcgagtatgg tcgagcgagt cctgcgcgtt
     2821 ctgcaccaga ttgtggatta cctggcgtat ttgcgcgccg tcggcccaga ccggcggcag
     2881 tcccggcgcg agccgcgtaa taatcggcgc gatcgaattc tcgtacaggc cgagcacctc
     2941 tcctaccagt gcattcagat cgagttgcgc cagttcgggc gccggcagcc gcgcgtattc
     3001 cgtgaaatca tcgaccatcg ccttcagcgc ggcgacctgg ttgacgatgg tctgggtcga
     3061 gcgccgcagg gtatcggctt cctcgtgcgg cagacggtca gcaagcttca tctcgaggcg
     3121 ttcggccgaa agctggattg gggtgagggg gttcttgatc tcgtgcgcca ggcgccgcgc
     3181 cacttcggcc caggcggtcg cgcgctgcgc ctgaatgagt tgcgtgatgt cgtcgaacac
     3241 cagcacgtag ccgcctcctg tctccttggg cagccgggcg ccacgcgcat gcagcatctt
     3301 gccggtgccc ttgagctgaa gctcgaattg ccagtccttg tcgccatgtt cgacgaaatg
     3361 ctcccgcatg ccttcagcga acgatgcgag ctcatcggcg aggatcgccg ccgcgccgtt
     3421 gttcgagatc gagagcttga gctcgtgatc gaataccagc accccggccg acaggttggc
     3481 aagtatgctc tcaagacgtg ccttggccgc ctcgagcgcc acgcggttcg cttccacgac
     3541 gtgccgcgcc tcgtccagct ggcgtgtcat cgagttgaat gattcggtaa gtatcccgag
     3601 ctcgtcgcgg ctggtgaccg gggcctgcct cgagaaattg ccgcgcgcca cggcttgcat
     3661 ggcctgcgcg agatgcgcga gcggttcggc gagcaggttt gactgcgtca ccgccaccga
     3721 caccgcgacg aacagcgcca tcagcaatga cagcgtgagc gtcaggatgt aaatccgttt
     3781 gagcccatcg cgcgaaatcg ccagctcctt gtagtcgcga taagccgctt cgaccgcttc
     3841 cgcgctgttg gaaaatgacg gcggcacgct ttgtcgcaac tgcagaaaac gcgcttcatc
     3901 ggcgaagctc cggctgttga tcggcacgat cacgcgcagc gacagtggtc gccctgcgac
     3961 cgcatcgacc gaggtgtagc cgcgcccgat gcgcgcctgg cgtagcgctt gggcccccgg
     4021 cagctccggg acgaatttcg tgacatcggc gctcgccccc gccaccaggc gcccgttcgc
     4081 cgccacgatg acggcctcct cgactgccaa ttgttcgcgt gcgcgttcga gctgcacggc
     4141 cagctgtgca gacggtcgat cggcgagctc gattgctgcg gcgcgcgctt tcgactgtat
     4201 atccaggatc atctggtcga tcgcctgttg accaaggcta atgccaccct cgagcgcggc
     4261 atccaccttg acgctgaacg acgactcgat gctgcgggtg ataaacagaa ccgacacccc
     4321 gtacacgatc agaccgggca cgaaagtaag cagggcgaac cgcacgaaaa gccggagtgt
     4381 tagccgcgcg ccgaacaccc gcgcgcggtg acgccgtgcg agcgcgatca gctggtagac
     4441 gacgatcgcg aacagcaggc ctgccagcgc agtgttcaga ccgagcgcca gcggatagtg
     4501 cctggcgaac aaggtcacat cggcgcttgc ggtcgcgagc aggaacagtc cggccgccgc
     4561 gactatcccc ccgatgatga gcagcagctt catcgctcct ccggccgctt cggctcacgg
     4621 ctttccaccg gagccggcaa ttgctgcggc gagcgcacca cgaaccgttt ccacggcgat
     4681 tcgaggtgca attcgcggct ggtgagcgcc gagagctgga acggcttggg cagaagcgtg
     4741 gtatccagtc gcatgcgcac tgccgcctca tagtccgatc tggagaaggc gaccgtgcgg
     4801 tcgaccacga gccagttgcg tacgcgcttg agcacgttga gcgcttcctc gagcgtggca
     4861 aaattctgct gcagcaggtt ctgctgctgc agcccggtag aaagccggta gtgtcgcgac
     4921 agcgcatgat aggacagccg catctgcatc aggctcgagg cactgcgctc gtcgaaccag
     4981 taccagcggc ggcgcgtcag ctcgaactcg acgcggaaat acagcggcac gccattggcc
     5041 accacttcgg cgaggcgcgg cgtcagctcg aatgcgaaat cggcgtccag caccagcccg
     5101 tcctccacgg cgcgcaaggc cgcgtcacgc acctcgatcg tgtcggcgcg cgcgggcccg
     5161 gctgcagtcc caagtaacgc aaggaatccg gtgagtaggg cgatgcggcg catcgcgctg
     5221 tctcaggctt gcttctggat cagcgcgtag tagaaaccgt cgtgctcggc atccggcagc
     5281 cattgcgcgg cggcgccgtc aggcagcggc agccgcagcg cgtcgggagt gcgcatgacg
     5341 aatgcatcga tcactgcgtc gttttcctgc ggaaacacag agcaggtgac atacagcaat
     5401 ttaccatccg ggccgagcac ctgccaaagt gcgtcgagga tcgaagcctg gcgcgcggcg
     5461 aaggcgggga tgtcctgcgc gcggcgcagc cactttaggt ccgggcggcg ccgcgcgatc
     5521 cccgacgccg agcaggggac gtcggcgagg atgcggtcga aacgctcgcc gttccaccag
     5581 ccggcaaggt gggtgcagtc cgctgccatc agcgttgcgt gcagccgcag ccgatcgaga
     5641 ttacgcccca tacgcccgcg acgctcgtca tcggagtcga ttgccgtgac ttcgacgtcg
     5701 gctgtttcga ggatatgaga agttttcccc ccaggtgcgg cgcaggcgtc caggacgtgc
     5761 tggcccgaga ccagatcgag gcagggcgcg gcgcgctgcg cgccggcgtc ctgtaccgaa
     5821 acctcgccgg catcgaatcc gggcaggcgc tcgaccggca ctggccgctc gagcagcagc
     5881 gcgtgctcgg caacgcgccg cgctgagata cccgatgccg cgagccgtgc ctggtaatcg
     5941 gccgtgccga cccgtcgccg gttgacgcgc aggcacatgg ggggatgcga attgcccgca
     6001 aggagcacat tcccccagca ctcggggtac gcgctccgca aggtctcggc ccaccatcgc
     6061 ggatgctgaa atcgtgcctc aaggtcggcg ccgatgcgcg actccaagga tgctcgctcg
     6121 cgtgcggcgc tacgcagcag cgcgttgacg tagccttttg ccgtccattt ttcgagcagc
     6181 gcgcaggcac gcacggcgtg gtcgaccacg gtgtactcgg cgtaccgacc cgactcgagg
     6241 gcatagagcg cgcaccacag caaagcttct accagggcat cgggacgccc gcggtgcgag
     6301 agctcgcgca cgatggcgtg cacgcgcccg tagcgccgca gcgttccatg cgtgagatcg
     6361 ataagcgcgg cgcgcggcgt ggcgctgccg tcgtcatcga gccggccgat ctgcccggaa
     6421 aggcttgctc cggccgcaac gcgagcgacc acggttgcgg ccttcgcgag cgcctgcgcc
     6481 agcgcgccag tggtcatccc aagcgcgcgc ccggcgaaag cgcatggccc cgcaggaaat
     6541 gcgcggcggc aaggcgtctg ccgcctgcac gctgcaattc tgttatggcg agcgcgccgt
     6601 cgcgacatgc cacgaccagt gcatcacccg ccgcaagcac agttccgggc tcgtcccgac
     6661 cggaaacgat gccggcgcgc catatcttga tcggctcgcc ttgaaacagg gtcgtcgctc
     6721 cgggtgcggg acgaaacgcg cgcaccgcgc gctcgagctc ggccgccgga agtgaccagt
     6781 tcaggatcgt gtccttgcgg tcgatcttgc gcgcataggt cgcgccggcc tcggactgct
     6841 cgaccgcgtg cgcgtggcca gcgtgaagcg cggcgagcgc ggcgacgatc gcttgcgcac
     6901 cgagcgcagc gagcttgtcg tgcagggttc cggcatcgtc gtcggcagcg ataggtaggc
     6961 ggtgctgcgc tagcaccggg cctgtgtcga gcccggcgtt cattcgcatg atggaaatgc
     7021 cggtctcgcg gtcgccggcg agcagggcgc gctggatcgg tgccgctccc cgccagcggg
     7081 gcagcagcga ggcatgaatg ttccacgcgc cgaacggcgc cagctcgagc acttggggcg
     7141 gcagcagcag tccataggcc gcgacggcga gcgcc
//
LOCUS       DASPXW010000025         2668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_764322, whole genome shotgun sequence.
ACCESSION   DASPXW010000025 DASPXW010000000
VERSION     DASPXW010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764322"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="VEL04_00605"
     CDS             <1..778
                     /locus_tag="VEL04_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020693804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYT46893.1"
                     /translation="WIAGRRGPVLESCSQELVSKYGGTVRTHAVDIRDAAAVDAMVQH
                     IWDEAGPLTGLVNNAAGNFISPTQSLTTNGFNAIANIVFHGTFYVTQAVGKRWIEGKH
                     KGSVVSILVTWVHTGSPYVVPSAMSKAGLHAMTKSLAVEWGRFGIRLNAIAPGPFPTE
                     GAAKRLRPGAGGFEDTTHMNPMRRIGRMEELQNLAAFLMADGCEWLTGETIAVDGGGY
                     LATGSGGYFLQLDKMSDADWEKARAMIKAQNDKDRAGRTA"
     gene            778..2394
                     /locus_tag="VEL04_00610"
     CDS             778..2394
                     /locus_tag="VEL04_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYT46894.1"
                     /translation="MVLVPRPAASLILLRDSAAGPEALMLQRTPSAVFLGGAHVFPGG
                     SLDEADADPRVLRRLLDLDAKDPPAPYRVAAVRECFEEAGILLAWDENRKQITAQRAD
                     SLMRYRNRPFVELLEAENLFIPASALAYYGHWITAPGRARRFDTRFFVAVAPEGQAGS
                     HDAAETVHDIWISPREALERGARGELELVFATQHTLHELKSFSSARQAFEQVFALPEV
                     EVNRACWAQGKEGAKLFRRADAAYFEIHWSDPDESGETTYDLAPGVPKRLDRWVQRII
                     APNPGVMTGPGTNTYLVGERDLAIIDPGPAIASHIEKILQFKNIKWILCTHTHMDHSP
                     AAAALKAATGAQVLGRPAPEGQDATFKPDFVVESGQRIQLGEASGSISLRAIHTPGHA
                     SNHLCYFLEETRMLFTGDHVMQGSTVVINPPDGDMRAYLASLEKLLAEELAIIAPGHG
                     YLIGAPHKELRRLIAHRLGREQKVVRALEQLGDASVEDLLPLVYDDVPARIHRVAARS
                     LTAHLEKLAADGAVRAAGERYALVKSRRAG"
     gene            2421..>2668
                     /locus_tag="VEL04_00615"
     CDS             2421..>2668
                     /locus_tag="VEL04_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT46895.1"
                     /translation="MANEEKTLRMVTNLDRGAIEAKLAEVRKTAQSKSLAELASMFAG
                     VEGMPRAQIETRVGNALKWLADKPQHQSISAQLELVEL"
BASE COUNT          468 a          914 c          888 g          398 t
ORIGIN      
        1 ctggatagct gggcggcgcg ggcctgttct ggagtcttgt tcgcaagaac ttgtttcaaa
       61 atatggcgga acggtgcgga cccatgccgt cgacatccgc gacgccgccg cggtcgacgc
      121 gatggtgcag cacatctggg acgaagccgg cccgctcacc ggcctggtca acaacgccgc
      181 cggcaacttc atcagcccga cccagtcgtt gaccacgaac ggcttcaacg ccatcgccaa
      241 catcgtgttc cacggcacgt tctatgtgac gcaggcggtc ggcaaacgct ggatcgaggg
      301 caagcacaag ggctcggtgg tctcgatcct ggtgacctgg gtgcataccg gctcgcccta
      361 tgtggtgccc tcggcgatgt cgaaggccgg gctgcacgca atgaccaagt cgctcgcggt
      421 cgaatggggc cgcttcggca tccggctcaa cgccatcgcg ccgggaccct tcccgaccga
      481 aggcgcagcg aagcgcctgc gccccggcgc cggcggtttc gaggacacga cccacatgaa
      541 cccgatgcgc cgcatcggaa gaatggagga gctgcagaat ctcgccgcct tcctcatggc
      601 cgacggctgc gagtggctca ccggcgagac catcgcagtg gacggcggcg gctacctcgc
      661 caccgggtcg ggcggctact tcctgcagct cgacaagatg agcgacgccg actgggaaaa
      721 ggcgcgcgcc atgatcaagg cgcagaacga caaggatcgc gccggccgca ccgcctgatg
      781 gtattggttc cgcgtcccgc ggcgtccctc atcctgctgc gcgactcggc cgcaggaccc
      841 gaagcgctga tgctgcagcg cacgccgagc gcagtatttc tcggcggcgc ccatgtcttc
      901 cccggcggct cgctcgatga agccgatgcc gatccgcgcg tcctgcgccg gctcctcgac
      961 ctcgacgcca aggacccgcc tgcgccctac cgggttgcgg ccgtgcggga atgtttcgag
     1021 gaagcaggga ttcttctggc ctgggatgaa aaccgaaagc agatcacggc gcagcgcgcg
     1081 gattcattga tgcgctatcg caaccggccg ttcgtcgagc tgctcgaggc cgaaaacctg
     1141 ttcatcccgg cgagcgcgct cgcctattac ggccactgga tcacggcgcc gggacgcgcg
     1201 cggcgcttcg atacgcgttt tttcgtcgcc gtcgcgcccg agggccaggc gggctcgcac
     1261 gacgcggcag agaccgtgca cgacatctgg atcagcccgc gcgaggcgct cgagcgcggc
     1321 gcgcgcggcg agctcgagct ggtgttcgcg acgcagcaca cgctgcacga gctgaagagc
     1381 ttctcgagcg ccaggcaggc cttcgagcag gtgttcgcgc ttcccgaggt cgaggtgaac
     1441 cgcgcctgct gggcgcaggg caaggagggg gcgaagctgt tccgccgcgc cgacgcggcg
     1501 tatttcgaga tccactggag cgacccggac gagagcggcg agacgaccta cgacctcgcg
     1561 ccgggcgtgc cgaagcggct cgatcgctgg gtacagcgca tcatcgcgcc gaaccccgga
     1621 gtgatgaccg gccccggcac caatacctac ctcgtgggcg agcgcgacct cgccatcatc
     1681 gaccccgggc ccgcgatcgc ttctcacata gaaaagattt tgcagttcaa aaacatcaag
     1741 tggatcctgt gcacgcacac tcacatggac cattcccccg ccgcggctgc gctcaaggcg
     1801 gcgaccggcg cgcaggtgct cgggcgcccg gcgcccgagg gccaggacgc caccttcaag
     1861 cctgatttcg tggtcgagag tggccagcgc atccagctgg gagaagcgag cggctcgata
     1921 agcctgcgcg ccatccacac gcccgggcat gcgtcgaatc atctttgcta cttcctcgaa
     1981 gagacgcgca tgctgttcac cggcgaccac gtcatgcagg gctccaccgt ggtgatcaat
     2041 ccgcccgacg gcgacatgcg ggcgtatctc gcctcgctcg agaagctgct cgccgaggag
     2101 ctcgcgatca tcgccccggg gcacggctat ctcatcggcg cgccgcacaa ggagctgcgg
     2161 cggctgatcg cgcaccgcct cggccgcgag cagaaggtgg tgcgcgcgct cgagcagctc
     2221 ggcgacgcct cggtcgagga cctgctgccg ctggtctacg acgacgtgcc ggcgcgcatc
     2281 caccgcgtgg cggcgcgctc gctcacggcg cacctcgaga agctcgccgc cgacggcgcg
     2341 gtgcgcgcgg cgggcgagcg ttacgcgctg gtaaaatccc gccgggctgg ctaagatcgc
     2401 tgctcgagga ggtttgcgcc atggctaacg aagagaagac gctgcggatg gtcaccaatc
     2461 tcgatcgcgg cgcgatcgaa gccaagctcg ccgaagtgcg caagacggcg caatcgaaga
     2521 gcctcgcaga gctcgcctcc atgttcgccg gggtcgaagg catgccgcgc gcgcagatcg
     2581 agacgcgggt cggcaacgcg ctcaagtggc tcgccgacaa gccccagcac cagagcatca
     2641 gcgcgcagct cgagctcgtg gagctcaa
//
LOCUS       DASPXW010000026         2147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_638130, whole genome shotgun sequence.
ACCESSION   DASPXW010000026 DASPXW010000000
VERSION     DASPXW010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638130"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VEL04_00620"
     CDS             <1..324
                     /locus_tag="VEL04_00620"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HYT46896.1"
                     /translation="EAEEEIGLPAGRVEIVARLPDYLTRTGFRVTPVVGLLTPPLELA
                     PDRREVEEVFEVPLAFLLDARNHLRETRELGGETVGYYVMQYRERRIWGATAGMLVNL
                     YRMLA"
     gene            321..1226
                     /locus_tag="VEL04_00625"
     CDS             321..1226
                     /locus_tag="VEL04_00625"
                     /inference="COORDINATES: protein motif:HMM:NF005792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobD/CbiB family protein"
                     /protein_id="HYT46897.1"
                     /translation="MSVLAIIAALVVEQWRPLGDRKGVAAALAGWAAWLEHSFNGGEH
                     RHGVVAWLVAVLPAVAAAIFLHILFSQMSALLALAFNIAVLYLTLGFRQFSHYFTDIQ
                     IAIKSGDVDRAGALLDAWRGAPGVVRTHEEVIRLAIEGALVASHRHVFGVLLWYLLLP
                     GPSGAILYRLAAYLASRWKDLGAFGRFAQAAFHVLEWPAVRLTAAAFAVVGDFEDAVY
                     CWRTQARSWPDPDAGIVLAAGAGAMGVRLGMALQEVDGIQARPELGVGEPAEGPFLDS
                     TVGLLWRALVLWVFTLLIVSVARLL"
     gene            complement(1228..>2147)
                     /locus_tag="VEL04_00630"
     CDS             complement(1228..>2147)
                     /locus_tag="VEL04_00630"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HYT46898.1"
                     /translation="SDVLFAGAVLVWLANTLASALRGSGNTLAPAAALALAALAQIPL
                     SGVLTLGWGPFPGLGIRGAALAYACAFGLATAAMGIYLCVGTLRPRGADWRLERRLFG
                     EILRVGALSSVSALQTVLTAVILTGCVGAFGTAALAGYGVGVRLELLQVPIVFAIGQA
                     LVVLVGTHVGAGRGARAKRIAWVGTLAAMSVSLAIGLAAALFPAAWVRIFSDDPGVLA
                     AGSLYLRIVAPFYLLFAAGMALYFAAQGAGRVGLPMLSGTARLAVVAAGGLMVIHAGA
                     PLAMLFAVIACGIALFGALTAAAVYKAPW"
BASE COUNT          317 a          769 c          775 g          286 t
ORIGIN      
        1 gaggccgagg aggagatcgg gctgccagcc gggcgcgtgg agatcgtggc gcggctcccg
       61 gactacctga cgcgcaccgg ctttcgcgtc acgccggtgg tcgggctgct cacgccgcca
      121 ctcgagctcg cccccgaccg ccgcgaggtc gaggaggtgt tcgaggtgcc actcgcattc
      181 ctgctcgatg cgcgcaatca cctgcgcgag acccgcgagc tgggcggcga gacggtcggc
      241 tactacgtca tgcagtaccg ggagcgcagg atctggggcg cgaccgccgg gatgctggtg
      301 aacctttata gaatgctcgc atgagcgtgc tcgcgatcat tgccgcgctg gtggtcgagc
      361 agtggcggcc gctcggcgac cgcaagggcg tcgccgccgc gctcgccggc tgggcggcct
      421 ggctcgagca ttcgttcaac ggcggcgagc acaggcacgg cgtggtggcc tggctggtgg
      481 cggtgctccc cgcggtcgct gccgcgatct tcctgcatat cctgttttcc cagatgagtg
      541 cattgcttgc gctcgccttc aacatcgcgg tgctgtacct gacgctcggc ttccgccagt
      601 tcagccatta cttcaccgac atccagatcg ccatcaagtc gggcgacgtc gaccgcgcgg
      661 gcgcgctgct cgacgcgtgg cgcggcgccc cgggggtggt gcgcacacac gaggaggtga
      721 tccgcctggc gatcgagggg gcgctggtcg cctcgcaccg gcacgtgttc ggcgtgctgc
      781 tctggtacct gctgctgccc gggccgagcg gcgccatcct gtaccggctc gcggcctacc
      841 tcgcctcgcg ctggaaggat ctcggcgcct tcggccgctt cgcgcaggcc gcgttccacg
      901 tgctcgagtg gcccgcggtg cgcctcaccg cggcggcgtt cgcggtcgtc ggcgacttcg
      961 aggacgcggt gtactgctgg cgcacgcagg cgcgctcgtg gcccgacccg gacgccggca
     1021 tcgtgctcgc cgccggtgcc ggcgccatgg gcgtgcgcct cggcatggcg ctgcaggaag
     1081 tcgacggcat acaggcccgg ccggagctcg gcgtcggcga gcccgccgag ggaccatttc
     1141 tcgacagcac cgtgggcctg ctttggcgcg cgctggtgct gtgggtcttc acgctgctca
     1201 tcgtcagcgt ggcgcggctg ctctgaatca ccacggcgcc ttgtagacgg cggcggcggt
     1261 gagcgcgccg aagagcgcaa tgccgcaagc gatgaccgcg aacagcatcg cgagcggcgc
     1321 cccggcatgg atcaccatca ggcctcccgc ggccaccact gccagacgcg ccgtgccgga
     1381 gagcatcggc agcccgacgc ggcccgcacc ctgcgcggcg aaatacagcg ccatgccggc
     1441 ggcgaacagc aggtaaaaag gcgcgacgat gcgcaggtac aggctgcccg ccgcgagcac
     1501 gccgggatcg tcgctgaaga tgcgcaccca cgccgccgga aacagagcgg ccgcgagacc
     1561 gatcgcgagg ctcacggaca tggcggccag cgtgccgacc caggcgatgc gcttcgcgcg
     1621 cgcgccgcgg ccggcgccga catgggtgcc gacgagcacg accagcgcct ggccgatcgc
     1681 gaacacgatc ggcacctgca gcagctcgag ccgcacgccc acgccgtagc cggcgagcgc
     1741 cgcggtgccg aaggcgccga cgcagccggt gaggatcacc gcggtcagca ccgtctgcag
     1801 ggcggagacc gaggacagcg cgcccacccg caggatctcg ccgaacaggc ggcgctcgag
     1861 ccgccagtcc gcaccccgcg gccgcagcgt tcccacgcac agatagattc ccatggcggc
     1921 cgtggcgagg ccaaacgcgc acgcataggc gagcgcggcg ccgcggatgc cgaggcctgg
     1981 gaacgggccc cagccgagtg tcagcacgcc cgagagcgga atctgcgcga gtgcagcgag
     2041 cgccagcgcg gccgccggag ccaaagtgtt gccgctgccg cgcagcgcgc tcgcgagcgt
     2101 attcgcgagc cacaccagca ccgcgccggc gaacagcacg tcggaat
//
LOCUS       DASPXW010000027         3207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1646859, whole genome shotgun sequence.
ACCESSION   DASPXW010000027 DASPXW010000000
VERSION     DASPXW010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1646859"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VEL04_00635"
     CDS             complement(<1..559)
                     /locus_tag="VEL04_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT46899.1"
                     /translation="MKRFVAAVVSLACLLVLARGMLYLLTSAEMGEVARLQGQERLWA
                     QSKAKVEEYTKQKLATQRQLIALDELRGRDRLRLFLKAVDAAHVENVWFDEIRYYRRD
                     NLPAGNLDSPPAVARAGIIVVPDKNVPAPGTAPKAPDVEQRVGIVGHAINHARLAQFM
                     RRLESQRGIADVSLVDTSPRSYPNAL"
     gene            complement(607..1740)
                     /gene="pilM"
                     /locus_tag="VEL04_00640"
     CDS             complement(607..1740)
                     /gene="pilM"
                     /locus_tag="VEL04_00640"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HYT46900.1"
                     /translation="MRDALRQSLGSLWPGRSRGASFRIGLQLGHERLNVAQMRQTASG
                     IAFRAVASASFGCTWDALQAEPRRLKQVVKRVWAAQPFSGKEVVACMPHDQLKIFSVD
                     YTSAEGQSDAEAIARKIKERVKGNVDGMVVDFAPVRQANADDPVKEAVVAAATREHVT
                     AFLELLAEAGLTVTALDIDPMALTRVVPWAGKGAGGEMRNVLLINFGSACSYLTVMWG
                     RRLMLDRAVEFSEQRLLARVKTVMDLPEPMAQQLLLEHGVDTTAGGADGIGQFSAAAK
                     EVLRPELLALKAEVSKTLVYAASKTRGESVDKIFLVGGVARYPGIAQLMGEDLAKPVE
                     VLNPFAAFPHRLTPTELAELWPHSGVAIATGLALRGVPGSWQN"
     gene            complement(1737..2870)
                     /locus_tag="VEL04_00645"
     CDS             complement(1737..2870)
                     /locus_tag="VEL04_00645"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HYT46901.1"
                     /translation="MKSLRTMWSSTDFGASELTRYASAVMCQEARQGIFVLGILTMLL
                     MAGLAALYHALGLGGAYPYTFGVLALLALHVAVSARAVTDIKVLHLLGMMLLAVSGLA
                     FVLLAHRYGVFTATLFSTVVLLFMVIPLVPWGLREALAAVGVIYLIFTGSTLSVAGRF
                     AHEALWTLQFLMLSAAVISLALVAFAIVVRKEHLEARFKLTAANEKLAEVSLQDPLTG
                     AWNRRFLDEHFGAIVARHAARGQACALGVLDVDEFKGLNDSYGHACGDRVLQRLVKAL
                     TQALGADEYVVRIGGDEFVLIMQEETAKPRLERALKGFGEREKGFPDLPAMPSVSIGM
                     ARVPREAPIPLREAYLLADKSLYAAKAGGRGRVIESTLRQEVA"
     gene            complement(2867..>3207)
                     /locus_tag="VEL04_00650"
     CDS             complement(2867..>3207)
                     /locus_tag="VEL04_00650"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HYT46902.1"
                     /translation="SGNIYGATWASRDYCCQWTPSWGGTYDTATTYATGRVESSGVSP
                     LPVGFSSGWVTWDLTALAQGWVDGVIENDGILVKSRYPGDITYKLEFLTKENNSVGGT
                     HRPQLVVVYQ"
BASE COUNT          537 a         1090 c         1042 g          538 t
ORIGIN      
        1 ccagagcgtt gggatagctc cgcgggctcg tatcgaccag gctcacgtcg gcgatgccgc
       61 gctggctctc cagcctgcgc atgaattgcg ccagcctggc gtgattgatc gcatgcccga
      121 cgattccgac gcgctgctcg acgtcgggcg ccttgggggc cgttcccggc gctggaacgt
      181 ttttgtcggg caccacgatg attccggcgc gcgcgacggc cggcggcgaa tccaggttgc
      241 ccgcgggcag gttgtcgcgg cggtaatacc tgatctcgtc gaaccagacg ttttcgacgt
      301 gcgcggcatc caccgctttc aggaagagcc gcaaacggtc ccgtccccga agctcgtcga
      361 gagcgatgag ctgcctctgc gttgcgagct tctgctttgt gtactcctcc accttggctt
      421 tgctctgcgc ccagagccgc tcctgcccct gcagccgggc cacttccccc atttcggccg
      481 aggtgaggag gtacagcatt cccctcgcca gcacgagcag gcaggcgagc gaaacgacgg
      541 cggcgacaaa ccgcttcacc cggcggagca gaatctggcg ctgggcgtag tccgcgggga
      601 tcaggtctag ttctgccatg acccggggac gccccgcaac gccaggccgg tggcgatcgc
      661 tacgcctgaa tgcggccaga gctccgccag ttccgttggc gtcaggcggt gcgggaatgc
      721 cgcgaaagga ttgagcacct ccaccggttt cgccaggtcc tcgcccatca attgcgcaat
      781 gcccggatag cgcgcgacgc cgccgaccag gaagatcttg tccacgctct cgccgcgcgt
      841 cttcgaggcg gcatagacga gggtcttgct cacctccgcc ttcagcgcca gcaattcggg
      901 ccgcaggact tccttcgccg ccgcgctgaa ctggccgatg ccgtcggcgc cgccggccgt
      961 ggtgtcgacc ccgtgctcga gcagcagctg ctgcgccatc ggttcgggca ggtccatcac
     1021 cgtcttcacg cgggcaagca gccgctgctc ggagaattcg acggcccggt cgagcatcag
     1081 gcgccgtccc cacatgaccg tgagatagct gcacgccgag ccgaagttga tgagcaggac
     1141 gttgcgcatc tcgccgcccg cgcccttgcc ggcccagggc accactcgcg tgagcgccat
     1201 ggggtcgata tcgagcgccg tgaccgtcag cccggcctcg gcaagcagct cgaggaaggc
     1261 cgtcacgtgc tcgcgggtgg cggccgccac cacggcctct ttcaccgggt catcggcatt
     1321 cgcctgtcgc acgggcgcga aatcgaccac catgccatcg acgtttccct tcacgcgttc
     1381 cttgatcttg cgcgcgatgg cttccgcatc cgattgccct tcggcgctgg tgtagtcgac
     1441 gctgaagatt ttcagctggt cgtgcggcat gcaggccacc acctccttgc cgctgaaagg
     1501 ctgcgccgcc cagacgcgtt tcaccacctg cttcagccgg cgcggctcgg cttgcagcgc
     1561 atcccaggtg catccgaagc ttgcggaagc gacggcgcgg aaagcgatgc ccgacgcggt
     1621 ctgccgcatc tgcgccacgt tcagccgttc gtggccgagc tgcaagccga tgcggaagct
     1681 cgcgccgcgc gatcgcccgg gccagagcga gccgagcgac tgccgcagcg catccctcat
     1741 gcgacctcct ggcggagggt ggactcgatc acccggcccc gccccccggc tttagcggcg
     1801 tacaaggact tgtctgcaag cagatacgcc tccctcagcg ggattggagc ttcgcgcggc
     1861 acgcgcgcca tgccgatgct gacgctcggc atcgccggca gatcggggaa gcctttttcg
     1921 cgttccccga accctttgag cgcgcgttcg aggcgcggct tcgccgtttc ctcctgcatg
     1981 atcaggacga actcgtcgcc gccgatgcga accacgtatt cgtcggcgcc cagcgcctgc
     2041 gtgagcgctt tgaccaggcg ctgcagcacg cggtctccgc aggcgtggcc gtagctgtcg
     2101 ttcagcccct tgaactcgtc cacgtccagg acgcccaggg cacaggcttg accgcgggcg
     2161 gcgtggcgcg cgacgattgc gccgaaatgc tcgtcgagaa aacggcggtt ccacgcgccc
     2221 gtgagcgggt cctggagaga gacctcggcg agcttttcgt tggctgccgt cagcttgaac
     2281 ctcgcctcga ggtgctcctt gcgcacgacg atcgcgaacg cgaccagcgc gagcgagatc
     2341 accgccgcgc tcagcatcag gaactgcagc gtccagaggg cttcgtgggc gaagcgtccc
     2401 gccacgctca gggtcgagcc ggtgaagatg agatagatga cgccgacggc ggcgagcgcc
     2461 tcgcgcagtc cccagggaac cagcggaatg accatgaaca gcagcacgac ggtgctgaac
     2521 agggtcgcgg tgaaaacgcc gtagcgatgg gcgagaagca cgaaggcgag cccgctcacc
     2581 gcgagcagca tcatgccgag aaggtggagg accttgatgt cggtgaccgc cctggcggaa
     2641 accgcgacgt gcagggcgag cagcgccagc acgccgaagg tgtacgggta cgccccgccc
     2701 aggcccaggg cgtggtagag agccgcgagc cccgccatca agagcatcgt cagtatgccg
     2761 aggacgaaaa tgccctgccg cgcctcctgg cacatcacgg cgctcgcata gcgcgtcagc
     2821 tcgctcgcgc cgaaatcggt ggaggaccac atggtgcgca gggatttcat tggtagacga
     2881 cgaccagttg cggccggtga gtgccgccaa cggagttgtt ctccttggta aggaactcta
     2941 acttgtacgt tatatcgccg gggtagcggc tcttcacgag gattccatcg ttctcgataa
     3001 cgccatcgac ccacccttga gcgagcgccg tcaagtccca ggtcacccag ccgctactga
     3061 agccgactgg cagcggggag acgccgctgc tttccacgcg accggtggcg tacgtggtgg
     3121 cggtgtcata ggtaccgccc caagaaggcg tccattggca acagtagtcg cgagatgccc
     3181 aggtggcgcc atagatgttt cccgatg
//
LOCUS       DASPXW010000028         2945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_765107, whole genome shotgun sequence.
ACCESSION   DASPXW010000028 DASPXW010000000
VERSION     DASPXW010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765107"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1380)
                     /locus_tag="VEL04_00655"
     CDS             complement(67..1380)
                     /locus_tag="VEL04_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HYT46903.1"
                     /translation="MPPLEAGLILLSLLLVLLIGGVWIAIALAACAWFGLQFFTSTPP
                     DVNLFQSFWGSSASWTLAALPLFVWMGEILYRTKLSDEMFAGLAPWLGWLPGRLMHVN
                     ILGCAIFGTVSGSSAATCATIAKVALPELKRRGYDEKTCLGSLAAAGTLGILLPPSII
                     MVVYAVAAEVSIIKVFLAGILPGIVLTLLFSGYIVAWALLNPGKTPKDDLQLSLREKL
                     YRSRNLVPCTVLIVFIVWVMVIGWATATEAAAYGVLGALVIAGWSGGLNRDSFWASLM
                     GATRLSCMIMFILAGASFLSSCMAFTGIPRALAGWVASLAPNRYMLIAILAGVYILLG
                     TALDGVSMIVLTTSIVIPMVEKAGFDLVWFSIFIVLLVEIAELTPPLGFNIFVLQTMT
                     GRDSNYVALASLPFFFMMVLCVALITLFPELATWLPDTLVGVTRK"
     gene            complement(1392..1925)
                     /locus_tag="VEL04_00660"
     CDS             complement(1392..1925)
                     /locus_tag="VEL04_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HYT46904.1"
                     /translation="MRRFLDRLYYGSGALAAACLASIAVLMLAQAFGREAGILIRGAE
                     DFVAWLCAASAFFALGHTFRNGELVRVGLWLDLLGARGRWYAELFSLGFTALFGAYMV
                     WAVARFVFESWKFNEVAQGLVRIPIWMPQMSFVLGVTIFLVAVIDELIVVLKKGKPSY
                     QVAEEARRAAGDFSETL"
     gene            complement(1930..2898)
                     /locus_tag="VEL04_00665"
     CDS             complement(1930..2898)
                     /locus_tag="VEL04_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HYT46905.1"
                     /translation="MMLRTAFVAAAALGALAAQAQTKWDMPTPYSDGEFHTRNVVLFV
                     EDVKKASNGALDIVVHANGSLIKHPDILRAVSTGQVNIAEFLLGQFGNEDPVFAVDNL
                     PFLAAGYDNAWKLYQAQKPTLEKKLQGRGLKLLYSVAWPGQGIYTKEPLKSVADLKGS
                     KFRTYSPQTARLAELLGASPTVVQVPDVPQMFATGAIQAMITSSATGTSTKAWEFVKN
                     YYTTNAMHPKNVVAVNTRAFERLSDAERKALTTAASKAETRGWAMSKVRELEANETLA
                     KNGMSVRDPDAAMKAAFTKVGEQMAVEWRKSAGAEGEAILKAYRAK"
BASE COUNT          552 a          958 c          978 g          457 t
ORIGIN      
        1 cggtgaagct cgcgtggctc atgcaccgtc cgatgccgca gccgacggcc gcgagcgggc
       61 tgcgccctat tttcgcgtga cgccgaccag cgtgtccggc agccaggtcg cgagctccgg
      121 gaagagggtg atcagcgcca cgcacaatac catcatgaaa aagaacggca gcgaggcgag
      181 ggcgacatag ttgctgtcgc gcccggtcat ggtctgcagc acgaagatgt tgaagcccag
      241 gggcggcgtc agctcggcga tctcgaccag cagcacgatg aagatgctga accagaccag
      301 gtcgaagccc gccttctcga ccatcgggat cacgatcgag gtggtgagca cgatcatcga
      361 gacgccgtcg agcgcggtgc cgaggaggat atagaccccg gcgaggatgg cgatcagcat
      421 gtagcggttc ggcgcgagcg aggcgaccca cccggcgagc gcgcgcggga tcccggtgaa
      481 cgccatgcac gaggacagga agctcgcccc ggcgaggatg aacatgatca tgcacgaaag
      541 ccgcgtcgcg cccatcaggc tcgcccagaa gctgtcgcgg ttcagcccgc ccgaccaccc
      601 ggcgatgacc agcgcgccca gcaccccgta ggcggccgcc tcggtcgcgg tcgcccagcc
      661 gatcaccatc acccagacga tgaacacgat cagcaccgtg cagggcacca ggttgcggga
      721 gcggtagagc ttttcccgca acgaaagctg aagatcatcc ttcggcgtct tgcccggatt
      781 cagcagcgcc caggccacga tgtagccgga gaacagcagg gtgaggacga tgccgggcag
      841 gatgccggcg aggaacacct tgatgatcga gacctcggca gcgaccgcgt acaccaccat
      901 gatgatcgac ggcggcagca ggatgccgag ggtgccggcg gcggcgagcg agccgaggca
      961 ggttttctcg tcgtaaccgc ggcgcttgag ctcgggcagc gccaccttgg cgatggtggc
     1021 gcaagtcgcg gccgaagagc ccgaaaccgt cccgaagatg gcgcagccga gaatgttgac
     1081 gtgcatcagc ctgccgggca gccagccgag ccagggcgcg aggcctgcga acatctcgtc
     1141 cgagagcttg gtgcggtaca ggatctcgcc catccagacg aacagcggca gcgcggcgag
     1201 cgtccacgag gcgctcgagc cccagaacga ctggaacagg ttgacgtcgg gaggcgtgct
     1261 ggtgaagaac tgcaggccga accatgcgca ggcagcgagc gcgatcgcga tccacacccc
     1321 gccgatcagc agcaccagca acaaagagag cagaatcagt cccgcctcga gcggcggcat
     1381 cagagcctca tttatagtgt ttccgagaag tctccggcgg cgcggcgcgc ttcttccgcg
     1441 acctggtagg agggtttgcc ctttttcagc acgacaatca gttcgtcgat gacggcgaca
     1501 agaaaaatcg tcaccccgag cacgaagctc atctgcggca tccagatcgg gatgcgcacc
     1561 aggccctgcg ccacttcgtt gaatttccag ctctcgaaca cgaagcgggc gaccgcccag
     1621 accatgtagg cgccgaacag cgcggtgaag ccgagcgaga acagctccgc gtaccagcgc
     1681 ccgcgcgcgc cgagcaggtc gagccacagc ccgacgcgca ccagctcgcc gttgcggaaa
     1741 gtgtggccga gcgcgaagaa cgcggacgcg gcgcacagcc aggcgacgaa gtcctcggcg
     1801 ccgcggatca ggatccccgc ctcgcggccg aacgcctgcg cgagcatcag cacggcgatc
     1861 gaagcgaggc aggcagcggc gagggcgccg ctgccgtagt aaaggcggtc gaggaaccgc
     1921 ctcaaactac tacttggccc ggtaggcttt gaggatcgcc tcgccttcgg cgccggcact
     1981 cttccgccat tccactgcca tctgctcgcc gaccttggtg aacgcggcct tcatggcggc
     2041 gtcgggatcg cgcacgctca tgccgttctt ggcgagcgtt tcattggctt cgagctcgcg
     2101 caccttggac atggcccagc cgcgcgtctc ggctttcgag gcggcagtgg tgagcgcctt
     2161 tctctccgcg tcggacaggc gctcgaacgc gcgcgtgttg accgcgacca cgttcttcgg
     2221 gtgcatggcg ttggtcgtgt agtagttctt gacgaattcc caggccttgg tcgaggtgcc
     2281 ggtcgccgac gaggtgatca tcgcctggat cgcgccggtg gcgaacatct gcggcacatc
     2341 gggcacctgc accacggtcg ggctggcgcc caggagctcc gccagccgcg cggtctgcgg
     2401 gctgtaggtg cggaacttgc tgcccttgag atcggcgacg ctcttcagcg gctccttggt
     2461 atagatgccc tggccgggcc acgccaccga gtagaggagc ttgagcccac gtccctgcag
     2521 cttcttctcg agcgtcggct tctgcgcctg atagagcttc cacgcgttgt cgtagcccgc
     2581 agcgaggaag ggcaggttgt cgaccgcgaa caccgggtct tcgttgccga actggccgag
     2641 caggaactcg gcgatgttga cctggccggt cgacaccgcg cgcaggatgt cgggatgctt
     2701 gatgagcgag ccgttggcgt gcaccacgat gtcgagcgcg ccgttgctcg ctttcttcac
     2761 gtcctcgacg aacaacacca cgttcctggt gtggaactcg ccgtcggagt agggcgtggg
     2821 catgtcccac ttggtctgcg cctgggccgc caacgcgcca agcgccgcgg cggcgacaaa
     2881 tgctgttcga agcatcatcg gtctcctcat ccggaacggg gcaatcttag ctcacttccc
     2941 tcaat
//
LOCUS       DASPXW010000029         4895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1647184, whole genome shotgun sequence.
ACCESSION   DASPXW010000029 DASPXW010000000
VERSION     DASPXW010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4895
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1647184"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3423)
                     /gene="hrpA"
                     /locus_tag="VEL04_00670"
     CDS             complement(<1..3423)
                     /gene="hrpA"
                     /locus_tag="VEL04_00670"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HYT46906.1"
                     /translation="MTHSLRFDPALPISARREEIRAAIEAHPVVIVCGETGSGKTTQL
                     PKILLEMGRGAAGRIGHTQPRRIAARSVAARIAEELGPRLAPLVGYKVRFDDALRPEA
                     VIKLMTDGILLAETQGDRELRQYGSIILDEAHERSLNIDFLIGYLQKLVKARSDLKLV
                     ITSATIDAERFSRHFEGAPVIEVSGRLYPVEVRHRPVSGDAEDTTREEEEQALADAVE
                     ELCREGPGDVLVFLPGEREIRDAAETLRKKALRNLEILPLYSRLSAAEQDRVFKPAGA
                     RRVVLATNVAETSLTVPRVRYVVDTGYARIKRYSYRNKVEMLRVEPISRAAAQQRAGR
                     CGRVANGVCVRLYSEEDFARRPAFTDPELLRSSLAAVILRAKSLGLGSVEEFPFLDPP
                     GPRAIADGYALLAELGAVDDKNELTETGKELARLPLDPRLARMLVAARTEGSLEQLLV
                     IAAALSVQDPRQRPMERAAAADERHARFADERSDFLALLKLWKLHAEAGLRRLCRDNF
                     LSYPRMREWRDVHAQLKKTLEEMRWPNSSANPQQPEGYRAIHRALLAGLLGNIGLKEG
                     DESAYLGARGIKFWLHPGSWARKPGRWIVAAELVETTRLYARTVAGIEPKWLEELGGH
                     LLKRDRQQPHWEKSRGQVVALERGALYGLPVYAQRRVNFAAFDPVGARDIFIRSALVE
                     GELETRAPFFAHNRRLVVEIERLEHKSRRPDILVDDALIHAFYDVRIPTAVASGADFE
                     RWRKQAEREQPKLLYLSREDLMRHEAAGITTRNFPPELELGANRFALEYRFEPGSPRD
                     GVTLIVPLALLNQVPAARCEWLVPGLLKEKVKALARSIPQRQRHKLGPLEEFAEVFCA
                     ALPPADIPLAAALARYVRSELDLEVPVDAFRPDSAPPHLQMNFRVMDDDGRQLAAGRK
                     LADLKLELGSRTEEILKHETPLSDGERFTGWTMGDLPEIMQIERGAQTLIGYPALVDA
                     GAGGQGGVSLQVFDSPEKAREMHRAGVRRLLAIAFRERIRDIEKTLARDIALATLKDD
                     VVAAALERTFLGDSLPMTQADFAQRVEEGRSRFSLIAQELARAAAGMLAEQAVLHRKI
                     DALEKSFPAVAQDVRRQLERLLAPGWLARTSWERLQHLPRYLKAASLRLDKLR"
     gene            complement(3420..3977)
                     /locus_tag="VEL04_00675"
     CDS             complement(3420..3977)
                     /locus_tag="VEL04_00675"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HYT46907.1"
                     /translation="MFTGIVQAVGKIVRLNPLEIDCGTLDLSDVEVGDSVCVQGVCLT
                     VTAITPRGFTTDVSAETLRVTTGFERPGAVNLEKSLALGDRLGGHLITGHVDGVGEVI
                     SRGGGILTFRLPKELSRYVARKGSICVDGVSLTVNRVQDDRFEVNLIPHTLEVTTLGR
                     LAAGSKVNLEVDLIARYLERMLPGG"
     gene            4013..>4895
                     /locus_tag="VEL04_00680"
     CDS             4013..>4895
                     /locus_tag="VEL04_00680"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HYT46908.1"
                     /translation="MQRQVEIIGAAWGLGGADPGCADAPAVLAPLLARRLAECGVAAA
                     AGPMLRPLAGERRKQFAVGKLCGLLASAVADSRRRGRLPCVLGGDHSCAGGTWSGVAR
                     TLHERGGGSLGLIWVDAHMDSHTPGTSHSGRLHGMPLAWLLGQDDDPLYGLASGVVQP
                     QHVCIVGVRSFEPEEAERLERLGVRVFLMDEIHGRGLEAVFEEALGIATTGTDSFGVS
                     IDLDVISPEEVPHVGTPVAGGLASVDLARVLQRFGGDPHLAAVELVEYSPRLDRDGHS
                     ARAAVDLLAAALCGPRED"
BASE COUNT          747 a         1700 c         1654 g          794 t
ORIGIN      
        1 gcgcagcttg tcgaggcgca gcgacgccgc cttgaggtag cgtggcaggt gctgcaaccg
       61 ctcccacgac gtgcgggcga gccatcccgg cgcgagcagc cgctcgagct gccgcctgac
      121 gtcctgcgcg accgccggga agcttttttc gagcgcatcg attttcctgt gcaagacagc
      181 ctgctcggca agcatgccgg ccgcggcgcg cgcgagctcc tgcgcgatca ggctgaagcg
      241 gctgcggccc tcttcgacgc gctgcgcgaa gtccgcctgc gtcatgggaa gagagtcccc
      301 gagaaacgtg cgctcgagcg ccgccgcaac gacgtcgtcc ttgagcgtgg caagcgcgat
      361 gtcgcgcgcc agggtctttt cgatgtcgcg aatgcgctcg cgaaacgcga ttgcaagcag
      421 gcggcgcacc ccggcgcgat gcatctcgcg cgctttctcc ggcgagtcga atacctgcag
      481 gctcacgccg ccctgcccgc cagcgccggc atcgaccagc gccggatagc cgatgagcgt
      541 ttgcgcgccg cgctcgatct gcatgatttc gggcaggtcg cccatcgtcc agcccgtaaa
      601 acgctcgccg tccgagaggg gggtttcgtg tttcagaatc tcctcggtgc gagatccgag
      661 ctccagcttc aaatccgcga gcttgcggcc cgctgcaagc tgcctgccgt cgtcgtccat
      721 caccctgaag ttcatctgca ggtgaggcgg cgcggaatcc ggccggaaag cgtctaccgg
      781 gacctcgaga tccagctcgc tgcggacgta gcgcgcgagc gccgcagcga ggggtatgtc
      841 cgccggcggc agcgccgcgc agaatacttc ggcgaactcc tcgagcggac cgagcttgtg
      901 gcgctggcgc tgcggaatcg atcttgcgag cgccttgacc ttttccttca gcaacccggg
      961 caccagccat tcgcagcgcg ccgccggcac ctggttgagc agcgcgagcg gcacgatgag
     1021 cgtcacgccg tcgcgcggcg agcctggctc gaagcgatac tcgagcgcga agcggttcgc
     1081 tccgagctcg agctctggcg ggaagttgcg cgtggtgatc cccgccgcct cgtgccgcat
     1141 caggtcctcg cgcgacaggt aaagcagctt cggctgctcg cgctcggcct gcttcctcca
     1201 gcgctcgaaa tcggcgccgc ttgcgaccgc cgtgggaatg cgcacgtcgt aaaaggcgtg
     1261 gatcagcgcg tcgtcgacca ggatgtccgg ccggcgcgac ttgtgctcga ggcgctcgat
     1321 ctccaccacc aggcgccggt tatgcgcgaa gaacggcgcg cgcgtctcga gctcgccttc
     1381 gaccagcgcc gagcggatga agatatcgcg cgcgcccacc ggatcgaagg ctgcgaagtt
     1441 cacgcggcgc tgggcgtaga ccggcaagcc gtacaacgcg ccgcgctcga gcgccaccac
     1501 ctggccgcgg cttttctccc aatgcggctg ctgccggtcg cgcttgagca ggtggccgcc
     1561 gagctcttcg agccacttcg gctcgatacc cgctacggtg cgtgcgtaca ggcgcgtggt
     1621 ctcgaccagc tcggccgcga cgatccatct gccgggcttc ctggcccacg accccggatg
     1681 gagccagaac ttgatgccgc gggcgccgag atacgcgctt tcatcgcctt ctttcagacc
     1741 tatattgccg agcagtccgg caagcagcgc gcgatggatc gcgcgatagc cctccggctg
     1801 ctgcggattc gccgacgagt tcggccagcg catttcctcc agtgttttct tcagctgcgc
     1861 gtggacgtcg cgccattcgc gcatccgcgg gtaggagaga aagttgtccc ggcacaggcg
     1921 ccgcaggccc gcttcggcgt gcagcttcca cagcttgaga agcgcgagga aatcggagcg
     1981 ctcgtcggcg aagcgcgcgt ggcgctcgtc ggccgcggcc gcgcgctcca tcggccgctg
     2041 gcgcgggtcc tgcaccgaga gggctgccgc gatcaccagc agctgctcga gcgagccctc
     2101 ggtgcgtgcc gcgaccagca tgcgcgcgag gcgcgggtcg agcggcaaac gcgcaagttc
     2161 ctttcctgtc tctgtcaatt cattcttgtc gtcaactgcc ccgagttcgg ccagcaacgc
     2221 ataaccgtcg gcgatcgcgc gcggccccgg cgggtcgagg aacgggaact cctcgacgct
     2281 ccccagtccc aggcttttcg cgcgcaggat cacggcggcg agcgacgagc gcagcagctc
     2341 cgggtcggtg aatgccggcc gccttgcgaa atcctcctcg gaatagagcc gcacgcagac
     2401 gccgttcgcc acccgcccgc agcgcccggc gcgctgctgc gccgccgccc gcgagatcgg
     2461 ctcgacgcgc agcatctcca ccttgttacg gtagctgtag cgcttgatgc gcgcgtagcc
     2521 cgtgtcgacg acgtagcgca cgcgcggcac cgtgagcgag gtctcggcga cgttggtcgc
     2581 aagaaccacg cggcgcgctc ccgcgggctt gaagacgcgg tcctgctccg cagccgacag
     2641 gcgtgaatac agaggaagaa tctcgagatt cctgagagcc ttttttctga gtgtttcggc
     2701 cgcgtcccgg atctctcttt ccccgggcag aaacaccagc acgtcacccg ggccttcgcg
     2761 gcaaagctcc tcgaccgcgt cggcgagcgc ctgctcctcc tcctcgcgcg tcgtgtcctc
     2821 ggcatcgccc gagaccggac ggtggcgcac ttcgaccggg tagagccggc cggagacctc
     2881 gatcaccggc gcgccttcga aatggcgcga gaagcgctca gcgtcgatcg ttgcagacgt
     2941 tatgacaagt ttcaggtcgc tcctggcctt gaccagcttc tgcagataac cgatgaggaa
     3001 atcaatgttg aggctgcgct cgtgcgcctc gtcgaggatg atcgagccgt actgccgcag
     3061 ctcgcggtcg ccctgggtct cggcgagcag gatgccgtcg gtcatcagct tgatcaccgc
     3121 ctccggccgc agggcatcgt cgaagcgcac cttgtagccc accagcggag cgagccgcgg
     3181 accgagctcc tccgcgatgc gcgccgcgac cgagcgcgcg gcgatgcgcc gcggctgcgt
     3241 atggccgatg cggccggctg cgccgcgccc catctcgagc aggatcttgg gaagctgcgt
     3301 ggttttcccc gacccggtct cgccgcagac gatgaccacc ggatgcgcct cgattgcggc
     3361 gcggatttcc tcgcgccggg cgctgatcgg aagcgcggga tcgaaacgca ggctgtgcgt
     3421 catcccccgg gaagcatgcg ctcgaggtaa cgcgcgatga ggtccacctc gaggttgacc
     3481 ttcgagcccg ccgcgagccg gcccagcgtg gtgacttcca gcgtatgcgg gatgaggttc
     3541 acctcgaacc tgtcgtcctg gacgcggttc accgtcaggc tcacgccatc gacgcagatc
     3601 gagcccttgc gggcgacgta tctcgaaagc tccttgggca gcctgaacgt gaggatgccg
     3661 cctccacggc tgatgacttc gccgacgccg tccacatgcc cggtgataag atggccgccg
     3721 agcctgtccc ccagggcgag cgatttttcc aggttcaccg cgcccggccg ctcgaagccg
     3781 gttgtgacgc gcagcgtctc ggcggagaca tcggtggtga agccgcgcgg ggtgatcgcc
     3841 gtcacggtca ggcagacgcc ctgcacgcag accgaatccc cgacttcgac gtcggacagg
     3901 tcgagcgtgc cgcagtcgat ttccagcgga ttaaggcgca cgatcttacc caccgcctgc
     3961 acgatgcccg tgaacatggc ggggaaggtt accgcaaagg acttgtctgg cgatgcaacg
     4021 gcaggtggaa atcatcggcg cggcatgggg cctcggcggg gcggaccccg gatgcgccga
     4081 tgcccctgca gtgctggcgc cgctgcttgc caggcggctg gctgaatgcg gcgtggccgc
     4141 cgccgccggg ccgatgcttc gcccgctcgc cggcgagcga cgcaagcagt tcgcggtagg
     4201 caagctgtgc ggcctgctcg cctcggcagt cgccgactcg cggcggcgcg ggcggctgcc
     4261 gtgcgtgctc ggcggcgatc acagctgcgc cggaggcacc tggagcggcg ttgcgcgcac
     4321 cctccacgaa agaggcggcg gctcgctcgg cctcatctgg gtcgatgccc acatggacag
     4381 ccatactccc ggaaccagcc acagcggacg gctgcacggc atgccgctcg cctggctgct
     4441 cggccaggac gacgatccgc tctacggcct cgcctcgggc gtggtccagc cgcagcacgt
     4501 ctgcatcgtc ggcgtgcgca gcttcgagcc ggaagaggcc gagcgcctcg aacggctcgg
     4561 cgtgcgggtg tttctgatgg acgaaatcca cggccggggg ctcgaggcgg tgttcgagga
     4621 agcgctcggc atcgccacta ccggcaccga cagcttcggt gtcagcatcg acctggacgt
     4681 gatcagcccc gaggaagtgc cgcacgtcgg caccccggtc gccgggggcc tcgcgagcgt
     4741 cgacctggcg cgcgtcctgc agcgattcgg cggcgatccg catctcgccg cggtcgagct
     4801 ggtcgaatat tcgccgcgtc tcgaccgcga cggccacagc gcgcgcgcgg cggtcgactt
     4861 gcttgccgcg gcgctatgcg ggccgcgcga ggatg
//
LOCUS       DASPXW010000030         2554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1270617, whole genome shotgun sequence.
ACCESSION   DASPXW010000030 DASPXW010000000
VERSION     DASPXW010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1270617"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            775..>2554
                     /locus_tag="VEL04_00685"
     CDS             775..>2554
                     /locus_tag="VEL04_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HYT46909.1"
                     /translation="MSAPSFRRDFISKPIFRWAQQVLPTLSDTEREAIEAGDVWWDAD
                     LFSGNPDWGKLLAVPPATLSEEEQAFLDGPVDELCRMLDEWRISWELRDLPPEVWNFL
                     KRHKFFAMIIPKTYGGLGFSAYAHSEVIRKLSTRSLTAAVTAMVPNSLGPGELLLHFG
                     TKAQQDYWLPRLAEGKEIPCFGLTSPEAGSDAASMTDSGVVCRGLWQGREVLGIRLNW
                     HKRYITLCPIATVIGLAFKLYDPDHLIGERHEIGITLALVPTSLPGVEIGRRHLPALM
                     VFQNGPTRGRDVFIPIDNVIGGVAQAGKGWKMLMSALAAGRGISLPSLSAAGAAFAAH
                     TTGAYARIREQFHVPIGRFEAIQERLGRMAATAYLIDAARRLTCAGIDHGHKPAVVTA
                     IMKEQATERLRVVANDAMDVHAGKAVQEGPLNYLGGFYRSVPIGITVEGANIVTRSLI
                     QFGQGAIRSHPYLLKEMTALEDPDRARGLEEFDRAFWGHLGHSLANAFRAWWRAWTGG
                     VLAPAPAAGRARGFYQQLGRYAAAFALAVDMALLTLGGALKRQEMLSARFGDILSELY
                     LLSAVLKRWDEEGRQRADLPLVAWCME"
BASE COUNT          408 a          872 c          828 g          446 t
ORIGIN      
        1 ggtggagcgc cgagaggcga gcgtcccgcg ttatgggacg caaggcgcct cgctaggcgc
       61 ctggcgtatc gcgtcataag cgcgttcacg cgcgtctgtg acgcacatga tacgccccac
      121 gggtgcccgc tggcacccga acgactctcg gcgctccgcc cacccctcga ttcggggtga
      181 gtgaagcaaa gatgcaaacc ccgggcgcag aaatgcgccg cgggaaggag gaaggctgtt
      241 tgtatggaga gttctgcagt acgcgctgct cgtcctcgtc ctgaggagag cgcatgtcaa
      301 agacgttcca cgaattcaaa ggcgcgtgcg cgcgtctcga aggatgagga cgagcgactc
      361 ggtttgccct catgcttcga gacgcatcgc agcgcgcttg ggctgtggaa gcgccagcgc
      421 tcgcgtcgcg ctgcgatgct cctcagcatg agggtgaggg tgcgcggcgc attttggcga
      481 aacgaagcca agcgggagcc tgcgtgtttg gtccgagcga acaccaacct gcggatgtgc
      541 gaaacgaccg ccggcgcgat ttcattgtta ccgattgtta tttacaatga gttttgcaac
      601 tgcaacgtgt cggtcgcatt tgagcgctca aattcccttc acagcgatgt ggttgaaaga
      661 gcccatgcgg cacgctgcgt cgtggtttag ggtggctttt ggacttgaag ctcccatgcc
      721 cactcgccgc atccgcgcag caccttcaga tccacccccc gggccgaggc ctcgatgagc
      781 gctccttcat tccgccgcga ctttatctcc aagccgatct tccgctgggc gcagcaggtg
      841 ctgcccacgc tctccgatac cgagcgcgag gcgatcgaag cgggcgacgt gtggtgggac
      901 gccgacctct tcagcggcaa tcccgactgg gggaagctcc tggccgtccc gccggcgacg
      961 ctgtcggaag aagagcaggc gtttctcgac ggccccgtcg acgagctctg ccgcatgctc
     1021 gacgagtggc gcatcagctg ggagctgcgc gacctcccgc ccgaggtgtg gaactttctc
     1081 aagcgccaca agttcttcgc gatgatcatt ccgaagacct acggcggctt gggcttttcc
     1141 gcctatgccc attcggaggt cattcgcaag ctctccacgc gctcgctcac cgcggcggtc
     1201 accgccatgg tgccgaactc gctcggaccc ggcgagctct tgctccactt cggcaccaag
     1261 gcccagcagg attactggct gccgcgcctc gccgaaggga aggagatccc ctgcttcggg
     1321 ctcaccagcc cggaggcggg ctcggatgcc gcctcgatga ccgattccgg cgtcgtgtgc
     1381 cgcggcctct ggcagggccg cgaggtcctc ggcatcaggc tcaactggca caagcgctac
     1441 atcacgctct gcccgatcgc gaccgtgatc gggctcgcgt tcaagctcta cgatcccgat
     1501 cacctcatcg gcgagcgcca cgagatcggc atcacgctcg cgctggtgcc gacgagcttg
     1561 ccgggcgtcg agatcggccg gcgccatctt cccgcgctca tggtgttcca gaacggcccg
     1621 acccgggggc gcgacgtctt catccccatc gacaacgtca tcggcggcgt cgcgcaggcc
     1681 ggcaagggct ggaagatgct gatgagcgcg ctcgccgccg gccgcggcat ctcgctgccg
     1741 tcgttgtccg ccgcgggcgc ggcgtttgcc gcccacacca ccggcgccta cgcgcgcatc
     1801 cgcgagcagt tccacgttcc catcggccgc tttgaagcga ttcaggagcg cctcggccgc
     1861 atggccgcga ccgcctatct catcgacgcg gcgcgccgcc tcacctgcgc cggcatcgat
     1921 cacggccaca agcccgcggt ggtgaccgcc atcatgaagg agcaggcgac ggagcggctg
     1981 cgcgtggtgg cgaacgacgc catggacgtg catgccggca aggccgtgca ggaaggaccc
     2041 ctcaactatc tcggcggctt ctatcgttcg gttcccatcg gcatcaccgt cgaaggcgcc
     2101 aacatcgtca cgcgcagcct catccagttc ggacaaggcg cgatccgcag ccatccctac
     2161 ctgctcaagg aaatgacggc gctcgaggat cccgaccgcg cccgcgggct cgaggagttc
     2221 gatcgcgcgt tctggggcca tctcgggcac agcttggcca acgcgtttcg cgcttggtgg
     2281 cgcgcctgga ccggcggcgt cctcgcgccg gcgccggcgg cgggacgcgc gcgcggcttc
     2341 taccagcagc tcggacgcta cgcggcggcc tttgcgctcg ccgtcgacat ggcgctgttg
     2401 acgctgggcg gcgcgctcaa gcgccaggag atgctctccg cgcgcttcgg cgacattctc
     2461 tcggagcttt atctcctctc cgccgtgctc aagcgctggg acgaggaggg gcgccagcgc
     2521 gccgacctgc cgctcgtggc gtggtgcatg gaat
//
LOCUS       DASPXW010000031         2275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_513372, whole genome shotgun sequence.
ACCESSION   DASPXW010000031 DASPXW010000000
VERSION     DASPXW010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513372"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..>2275)
                     /locus_tag="VEL04_00690"
     CDS             complement(30..>2275)
                     /locus_tag="VEL04_00690"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYT46910.1"
                     /translation="AEGVSFMSMAIGKDKSQLAGVKAVTENYPLRGRLRIAPRPGAPD
                     APAARGPARGTVWLEERLVTALGAPVGSRIRLGGAALEVAAVLTQEPERSANFFNIAP
                     RLLMHGADVTATGLIRPGSRAWYYLYAAGAPQQVSLFENWARPRLERGQRIDTLESGR
                     PEVRAAIDRAERYLGLSALLAAVLAGVAIALATRRFVERHLDGCAVMRCLGATQAKLV
                     RLFGMEFMLLGLASAAAGLALGLAAQQAVAATLESLLRASLPAPGALPALQGFLVGLV
                     LLLGFALPPLIQLKNVPAVRVLRRESGAPRRGTIGAYAAGLASLAALLLWQAGDLKLG
                     GYVVGGFGAALAVFFVVSWGALRLLASRVLISKIRNRSLRYGLANLRRHAHGNAVQVA
                     SLALGLAAVLLLSFTQKDLVDAWRRAAPPDAPNRFLVGVQPGELQPVQRFLAAHGIAP
                     AEFYPMVRGRLMAVNGKPVSEADFSDERAKRLVEREFNLSYRDELPSHNSLAAGRWLK
                     LDENELSAEEGIAKMLGWKLGDELTLSVGGEPFSARITSLRKLRWDSMKVNFFVIAPP
                     RLLQGYPASFVSAFRVEPQNAAALDQLAARHPTLAVVDVGAAVRQLQGVIEQLIGAVR
                     FLFLFTLGAGLLVLYSALVATEDERRREAAVMRVYGASRAQVAGSQRAEFLAMGALAG
                     LLATLGAAAIGEVVAQRVFELDLPPSLGLWLAGPLAGLLLLSLNAWLSSRKVLRASPA
                     LTLRDSV"
BASE COUNT          364 a          825 c          819 g          267 t
ORIGIN      
        1 cgccacgcag gccgccgcgt caaccgggat cacaccgaat cgcgcagcgt cagcgccggc
       61 gaggcgcgca gcaccttgcg cgaggagagc caggcgttga gcgaaagcag cagcaggccg
      121 gcgagcggac cggcgagcca caggccgagg ctcggcggca ggtcgagctc gaacacgcgc
      181 tgcgcgacca cttcgccgat cgcggcggcg ccgagcgtcg cgagcagccc ggcgagcgcg
      241 cccatggcga ggaactcggc gcgctggctg cccgcgacct gggcgcgcga ggcgccgtag
      301 acgcgcatca ccgccgcctc gcggcggcgc tcgtcctcgg tcgcgacgag ggcggaatac
      361 agcaccagca gcccggcacc gagcgtgaag agaaacagga agcgcaccgc gccgatcagc
      421 tgctcgatca cgccctggag ctggcgcacc gctgcgccca cgtcgaccac ggcgagggtg
      481 ggatggcgcg cggcgagctg gtccagcgca gcggcgttct gcggctcgac gcggaacgcg
      541 ctcacgaagc tcgccggata gccctgcagc aggcgcggcg gcgcgatgac gaagaagttc
      601 accttcatcg agtcccagcg cagcttgcgc agcgaagtga tgcgcgcgct gaagggctcg
      661 ccccccaccg agagcgtgag ctcgtcgccg agcttccagc cgagcatttt ggcgatgccc
      721 tcctccgccg acagctcgtt ttcatcgagc ttgagccagc gtcccgcggc cagcgagttg
      781 tggctcggaa gctcatcgcg gtaagacagg ttgaattcgc gctcgaccag gcgcttggcg
      841 cgctcgtcgc tgaaatcggc ctcgctcacc ggcttgccgt tcaccgccat gagccgcccg
      901 cgcaccatcg ggtagaactc ggcaggtgcg atgccgtgcg cggcgaggaa gcgctgcacc
      961 ggctgcagct cgcccggctg cacgccgacc agaaagcggt tgggcgcgtc gggcggcgcg
     1021 gctcgccgcc aggcatccac caggtccttt tgcgtgaagc tgaggagcaa taccgcggcg
     1081 agcccgagcg ccaggctcgc cacctgcacc gcgttgccgt gcgcgtggcg cctgagattg
     1141 gcaagaccgt agcgcaagct ccgatttctt atcttcgaga tcaaaacgcg ggaggctaaa
     1201 agcctgagcg cgccccacga cacgacgaag aacacggcga gcgcagcgcc gaagccgccg
     1261 acgacgtagc cgccgagctt caggtcgccc gcctgccaga ggagcagcgc cgcgagggaa
     1321 gcgagccccg cggcgtaagc gccgatcgtc ccgcgtcgcg gcgcgcccga ttcgcgccgc
     1381 agcacgcgca ccgccggcac gttcttcagc tggatgagcg gcggcagcgc gaagccgagc
     1441 agcagcacca gcccgacgag aaatccctgc agcgcgggaa gcgcgccggg cgcgggcagg
     1501 ctcgcgcgca gcaggctttc gagcgtcgca gcgacggcct gctgggccgc cagcccgagc
     1561 gccaggcccg ccgcggcgga ggcgaggccg agcagcatga attccatgcc gaagagccgc
     1621 accagcttcg cctgcgtcgc gccgaggcag cgcatcaccg cgcagccgtc gaggtggcgc
     1681 tcgacgaagc gccgcgtggc gagcgcgatg gcgaccccgg cgagcaccgc ggcgagcagc
     1741 gcgctgaggc cgagatagcg ctcggcgcga tcgatcgccg cgcgcacttc gggccggccg
     1801 ctttccagcg tatcgatgcg ctgcccgcgc tcgaggcgcg gcctggccca gttctcaaaa
     1861 agtgaaacct gctgcggagc gccggccgca tacaggtagt accaggcgcg gctgccgggg
     1921 cggatcagcc cggtcgcggt gacatcggcg ccgtgcatca agaggcgcgg cgcgatgttg
     1981 aagaagttgg cgctgcgctc gggctcctgc gtcagcactg ccgcgacctc gagcgcggcg
     2041 ccgccgaggc ggatgcgcga gcccaccggc gcgccgagcg ccgtcaccag gcgctcctcg
     2101 agccataccg tgccgcgcgc cgggccgcgc gccgccggcg cgtcgggcgc tcccgggcgc
     2161 ggcgcgatgc gcaggcgccc gcgcagcggg tagttctcgg tcaccgcctt gaccccggcg
     2221 agctggctct tgtccttgcc gatcgccatg ctcatgaagc tgacgccctc ggcgc
//
LOCUS       DASPXW010000032         3993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_387566, whole genome shotgun sequence.
ACCESSION   DASPXW010000032 DASPXW010000000
VERSION     DASPXW010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387566"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1448)
                     /locus_tag="VEL04_00695"
     CDS             complement(<1..1448)
                     /locus_tag="VEL04_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HYT46911.1"
                     /translation="MERATTQQLAMLPIEFKLNGETVVGRPDEPIITTAKKHGVSIPH
                     LCHGGNQRSDGNCRACVVEIKGERVLAPACCRFPAAGMEVTSDSPRALTSQKMVLELL
                     LSDMSEEAYTLENKVLTWAKRLGVGKPRFSPRKQPKADLSHPAIAVNLDACIQCTRCV
                     RACREVQVNDVIGYAGRGDHSKIVFDFDDPMGESTCVACGECVQACPTGALMPARKVG
                     LQKIDKTVDSVCPFCGVGCLLTYHVKENKILFVTGKDGPSNQERLCVKGRYGFDYAHH
                     PHRLTKPLIRKNNVKKAADFKVDPGNWSAVFREASWDEALDLAASGLQRIKQKAPGAL
                     AGFGSAKGSNEEAYLFQKLVRTGFGTNNVDHCTRLCHASSVAALLEGIGSGAVSNQVN
                     DVANAEVIFLIGANPTSNHPVAATWMKNAAKSGVKLIVADPRRGELARHATHVLQFKA
                     DTDVALLNAMIHTIIDENLVDKKFVADRTSGF"
     gene            complement(1448..3115)
                     /locus_tag="VEL04_00700"
     CDS             complement(1448..3115)
                     /locus_tag="VEL04_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HYT46912.1"
                     /translation="MKRKGRFSPKGRALDPQALEEVRALLGDASRQRDLLIEHLHRLN
                     DRYGSLSAARLAALAQEMRLPLAEVYEVATFYHHFEVVKEGPDGKMRPPPALTVRVCD
                     SISCELAGSEALLAKLPSLLGKEIRVLRAPCVGRCEVAPCVVVGQNPIGNATIEAVQK
                     AASSGKTSCEETRYINFTAYRKQGGYQLIADCLAGKRSRDELTKIMEDSGLRGLGGAG
                     FPAGRKWRIVAAEPEPRLMAVNIDEGEPGTFKDRWFLERDPHRFLEGMLVAAWCAGVS
                     EIYIYLRDEYAGCRSVLERELQRLRADPPCALPPIFLRRGAGAYICGEESAMIESIEG
                     KRGMPRLRPPYVAQVGLFGRPTLEHNMETVYWVREIVEKGAAWFAGQGRNGRKGLRSF
                     SVSGRVKKPGVHVAPAGITVVQLINEYCGGMLDGHEFYGYLPGGASGGILPASKADIA
                     LDFDTLQPHGCFIGSAAVVILSNKDKASKAAVNLMKFFHDESCGKCTPCRVGTGKAAA
                     LMVERKWNQPLLNELSQAMMDASICGLGQAAPNPALSVMKYFPHEVS"
     gene            3185..>3993
                     /locus_tag="VEL04_00705"
     CDS             3185..>3993
                     /locus_tag="VEL04_00705"
                     /inference="COORDINATES: protein motif:HMM:NF024628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HYT46913.1"
                     /translation="MCRALLYLGEPVLLDNLLFQPDSALVRQSYMPKMLNLLNLAGFG
                     LRAWDPGSHAPGEPYFYGSSSLPVFDRNLKNLAEKVRAACVLAHVRGVAYSTAVDISL
                     QNVHPFQFEGLQWSLAHNGDLAGIQEMKPLLSPEIKPRYLARIRGTTDSEWIYALFVS
                     QLPDPSGPASDRQVFDALEKTLGIIRRARERLGIALSSSVNLFIANGSQLAALRYCFD
                     FGCYRTDDPARVHEANLSFLSLWYTLGRDYGLHDEEWKMTGGAERADSILVA"
BASE COUNT          683 a         1325 c         1227 g          758 t
ORIGIN      
        1 tcaaacccgc tagttctatc ggcaacaaat ttcttgtcaa ccagattctc atcgatgatc
       61 gtgtggatca tcgcgttgag gagcgcgacg tcggtatcgg ccttgaactg caggacgtgc
      121 gtggcgtggc gcgcgagctc gccgcggcgc gggtcggcga cgatcagctt gacgccgctc
      181 ttcgccgcgt ttttcatcca ggtcgcggcg accggatggt tcgaggtcgg gttggcgccg
      241 atcaggaaga tcacctcggc gttcgccacg tcgttcacct gattggacac ggcacccgag
      301 ccgatgccct ccagcagcgc cgccaccgac gaggcgtggc aaaggcgcgt gcaatggtcg
      361 acgttgttgg tgccgaagcc ggtgcgcacc agcttctgga acaggtacgc ctcctcgttc
      421 gagcccttcg ccgagccaaa gcccgccagc gcaccggggg ccttttgctt gattcgttgc
      481 aacccggacg cagccagatc cagcgcttcg tcccagctcg cctcgcggaa cacggccgac
      541 cagtttcccg ggtcgacctt gaagtcagca gcttttttta cattattttt tcttattaag
      601 ggcttcgtaa ggcggtgtgg atggtgggcg taatcgaagc cataacgccc tttcacgcac
      661 aggcgctcct ggttcgacgg gccgtccttg ccggtgacga aaaggatctt gttttccttg
      721 acgtggtagg taagcaggca gcccacgccg cagaacgggc acaccgagtc gaccgtcttg
      781 tcgattttct gcaagccgac cttgcgcgcc ggcatcagcg cgccggtcgg gcaggcttgc
      841 acgcattcgc cgcaggcaac gcaggtggac tcgcccatcg ggtcgtcaaa gtcgaacacg
      901 atcttcgaat ggtcgccccg tccggcatag ccaatgacgt cgttcacctg cacttcgcgg
      961 caggcgcgca cgcagcgcgt gcactggatg caggcatcca ggttcaccgc gatcgcggga
     1021 tgggaaaggt ctgccttggg ctgtttacga ggcgaaaagc gcggcttgcc aacccccaaa
     1081 cgcttcgccc aggtgaggac cttgttctcc agcgtgtagg cctcctcgga catgtccgag
     1141 agcagcagct cgagcaccat cttctgcgag gtgagcgcgc gcggcgaatc gctcgtcact
     1201 tccatgccgg cagcaggaaa gcggcagcag gcaggcgcca aaactctttc gcccttgatc
     1261 tcgaccacgc aggcgcggca attcccgtct gagcgttgat ttccgccgtg gcatagatga
     1321 ggaatcgaaa caccgtgttt tttcgcggtg gtgatgatcg gctcgtcggg ccggccgacc
     1381 acggtctcgc cgttgagctt gaactcgatc ggcagcatcg ccagctgctg agtggtggct
     1441 cgctccatca cgacacctcg tgcggaaaat acttcatgac gctgagcgcc gggttcggcg
     1501 ccgcctgccc caggccgcag atcgacgcgt ccatcattgc ctgtgagagt tcgttgagca
     1561 gcggttggtt ccacttgcgc tcgaccatca gcgcggccgc cttgcccgtg ccgacgcggc
     1621 agggcgtgca cttgccgcag gactcgtcgt ggaagaactt catcaggttc accgccgcct
     1681 tcgaagcctt gtctttgttg gaaagaatca ctactgccgc ggagccgatg aagcagccgt
     1741 ggggctgcag cgtatcgaag tccagcgcaa tgtcggcttt cgacgccggc aggatgccgc
     1801 ccgaggcgcc gcccggcaga tagccgtaga actcgtgccc gtcgagcatc ccgccacaat
     1861 attcatttat cagttgcaca acggtaattc ctgctggagc gacatgcacg ccgggttttt
     1921 ttacccgccc ggagaccgag aacgagcgca ggcccttgcg gccgttcctg ccctggcccg
     1981 caaaccacgc ggcgcccttc tccacgatct cgcgcaccca gtagaccgtc tccatgttgt
     2041 gctcgagggt gggacggccg aacaggccga cctgcgcgac gtagggcggc cgcagccgcg
     2101 gcatgccgcg cttgccctcg atcgactcga tcatcgccga ctcctcgccg cagatgtacg
     2161 ctcctgctcc tctccttaag aatatcgggg gaagcgcgca aggcggatcg gcccgcaacc
     2221 tctgcaattc cctttcgaga accgagcggc atcctgcata ttcatcacgc aggtagatgt
     2281 agatctcgct gaccccggcg caccaggcgg cgaccagcat gccttcgaga aagcggtgcg
     2341 gatcgcgctc gagaaaccag cggtccttga acgtgcccgg ctcgccctcg tcgatgttca
     2401 ccgccatcag gcgcggctcg ggctcggccg cgacgatgcg ccacttgcgc ccggcgggaa
     2461 agccggcgcc gccgaggccg cgcaagccgg aatcttccat gatcttggtg agctcatcgc
     2521 ggctgcgctt tccggcgagg cagtcggcga tcagctggta gccgccctgc ttcctgtacg
     2581 cggtgaagtt gatgtagcgc gtttcctcac agctcgtttt ccctgaagaa gctgcctttt
     2641 gaacagcttc gatcgtcgca ttccctatcg ggttctgtcc caccaccacg cagggcgcca
     2701 cttcgcatct gccaacgcag ggagcgcgaa gcacgcggat ttcctttccc aacagggaag
     2761 gcaatttggc aagaagagct tcagagcccg ccagctcgca ggagatcgaa tcgcaaacgc
     2821 gcactgtgag cgcgggaggc ggccgcattt tgccgtccgg gccttccttc accacctcga
     2881 agtgatggta gaaggtcgcg acctcgtaca cctcggcgag cggcaggcgc atttcctgcg
     2941 ccagcgcggc gaggcgcgcg gccgacaggc tgccgtagcg gtcgttcagc ctgtgcaggt
     3001 gctcgatcag caggtcacgc tgccgggaag cgtcgccgag cagggcgcgc acctcttcga
     3061 gcgcctgcgg atcgagcgcc ctgcccttgg ggctgaacct tcccttgcgt ttcatggaga
     3121 acgctattat cggccagcgg cgagcgcggg ttcttgactg cggtcaagcg catatacttc
     3181 ggcgatgtgc cgcgcgctgc tctacctcgg cgagcccgtg ctgctcgaca acctgctgtt
     3241 ccagcccgat tcggccctgg tgcggcagag ctacatgccg aagatgctga accttctcaa
     3301 cctggcggga ttcggcctgc gcgcctggga ccccggctcg cacgcgcccg gcgagcccta
     3361 tttctacggc tcgtcctcgc tgccggtgtt cgaccgcaac ctgaagaacc tggcggagaa
     3421 agtgcgcgcc gcctgcgtgc tcgcgcacgt gcgcggcgtc gcgtattcca ccgcagtcga
     3481 catttcgctg cagaacgtcc acccgttcca gttcgagggc ctgcagtggt cgctcgcgca
     3541 caacggcgac ctcgccggca tccaggaaat gaagcccctg ctttcaccgg aaatcaagcc
     3601 tcgatacctg gcgcgcatcc gcggcaccac cgacagtgaa tggatctatg cgctgttcgt
     3661 ctcgcagctt cccgacccgt ccggcccggc gagcgaccgg caggtgttcg acgcgctcga
     3721 gaaaaccctg ggcatcatcc ggcgcgcgcg cgagcggctc ggcattgcgc tatcttcgtc
     3781 ggtgaacctg ttcatcgcga acggcagcca gctcgcggcg ctgcgctact gcttcgactt
     3841 cggctgctac cgcaccgacg acccggcgcg cgtgcacgag gcgaacctca gcttcctttc
     3901 gctctggtac acgctcgggc gcgactacgg gctgcacgat gaggagtgga aaatgaccgg
     3961 cggcgccgaa cgcgccgatt cgatcctggt cgc
//
LOCUS       DASPXW010000033         2522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1271231, whole genome shotgun sequence.
ACCESSION   DASPXW010000033 DASPXW010000000
VERSION     DASPXW010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271231"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..116
                     /locus_tag="VEL04_00710"
     CDS             15..116
                     /locus_tag="VEL04_00710"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT46914.1"
                     /translation="MGDAKLEAEGKADKAAGKVQNAVGGLKDALRGK"
     gene            158..622
                     /locus_tag="VEL04_00715"
     CDS             158..622
                     /locus_tag="VEL04_00715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYT46915.1"
                     /translation="MKIAQIAPLYEAVPPSLHGGTERVVAHLTNALVELGHEVTLFAV
                     AEAHTRATLVPVRDRAIRLDPCPLKSDLAAHLSMLHELHSRRHEFDLLHFHVDLIHFP
                     FFEDFAARTVTTLHGRLDLEDLPEVYSRWRDYPLVSISDDLRTQIGAPRYRS"
     gene            684..2219
                     /locus_tag="VEL04_00720"
     CDS             684..2219
                     /locus_tag="VEL04_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYT46916.1"
                     /translation="MAQDQAKPSGPDLVQGIALAELQEGRMLAGHAAGEQVLLVRRGT
                     EVFAVGAHCTHYHAPLVDGLVVDGTVRCPWHHACFDLRTGEALRAPALSPVESWLVEQ
                     RDGRIFVREKRAQPEPKPGGKPAGAAPQKIVVVGGGAAGFAAVEMLRREQYQGTIVML
                     SNDDAPPVDRPNLSKDYLAGKAPEDWVPLRPDSFYSESGIDLRLNVDVTGIDVRSREV
                     VLADKSKVPYDRLLLATGAEPIRLSIPGADKPHVRMLRSLADCRAIIERAKTARRTVV
                     LGASFIGLEVAAALRARELEVHVVAPDKRPMERILGPQMGDFVRALHEEHGVVFHLEE
                     TAGAIDGKQVKLKGGGTLEADFVVAGIGVRPRIELAERAGLAVDRGVVVNAYLETSAP
                     GFFAAGDIARWPDPHSGESIRVEHWVVAERQGQTAARNMLGHREKFTAVPFFWSQHYD
                     IPINYVGHAEKWDELAIEGDIAGKDCLLRFRRDRRTLAVASIFRDIESLRAEAAMERE
                     VAL"
BASE COUNT          454 a          796 c          828 g          444 t
ORIGIN      
        1 cggcaggcaa agcgatcggc gatgccaagc tcgaagccga aggcaaagcc gacaaggcgg
       61 caggcaaggt gcagaacgcc gtcggcggct tgaaggacgc gcttcgcggc aaatagtagc
      121 gagtgaggcc ggctgccgat cggattcacg gcatcttatg aagatcgctc agatcgcgcc
      181 gctgtatgag gcggtgccgc cgagcctgca tggcggcacc gagcgcgtcg tagcgcacct
      241 gaccaacgct ctcgtggagc tcgggcacga ggttacgctg ttcgcggttg cggaggccca
      301 tacacgggcg acgctggttc cggttcgcga tcgagccatt cggctcgacc cgtgtccgct
      361 gaaatcggac ctggccgctc acctgtccat gctgcatgaa cttcattcgc gccggcacga
      421 attcgatctt ctgcatttcc atgtcgatct catccatttt ccgttcttcg aagattttgc
      481 ggcgcgaacc gtgaccacgc tgcacggtcg actcgacctg gaggacctgc cagaggtcta
      541 ttcgcgttgg cgcgactatc ccctcgtctc gatatcggat gatctccgca ctcagattgg
      601 cgcgccgaga tatcgttcgt gaagaaactg cggttatcgg cgtcacggac gaaaccgatc
      661 caggaacctc agggagaaga gtaatggctc aagatcaagc taagcccagc ggccccgatc
      721 tggtgcaggg cattgcgctc gctgagcttc aggagggccg catgcttgcc ggtcatgcgg
      781 ccggagagca ggttctgctg gtgcgccgcg gtacggaggt gttcgccgtt ggggcccatt
      841 gcacgcacta ccacgcgccg ctcgtcgatg gactcgtcgt tgatggcacc gtgcgctgtc
      901 cctggcatca cgcctgcttc gacctgcgga cgggcgaagc cttacgcgcg ccggcgttga
      961 gcccggttga gtcgtggttg gtcgagcagc gcgatggcag gatcttcgtg cgcgagaagc
     1021 gcgcacagcc ggaaccgaag cccggcggca agccggccgg cgcagcgccg cagaagatcg
     1081 tggtcgtcgg cggaggtgcc gccggattcg cggccgtaga gatgctccgg cgcgagcaat
     1141 accagggcac tatcgtcatg ctgagcaatg acgacgcgcc gcccgtagac cggccgaacc
     1201 tctccaagga ctaccttgcc ggcaaggcgc cggaggattg ggtcccgctg cgcccggaca
     1261 gcttctattc cgagagcggc atcgacctgc gcctcaatgt ggacgtcacc ggtatcgacg
     1321 tgcgttcgcg ggaagtcgtg ctcgccgaca agagcaaggt tccttacgac cggctgctgc
     1381 tggcaaccgg cgccgagccg atccgcctgt ccatcccggg cgccgataag ccgcacgtgc
     1441 gcatgctgcg ctcgctcgcc gactgtcgag ccatcatcga gcgtgccaag acggcgcgcc
     1501 ggacggtcgt gctgggtgcg agctttatcg gactcgaggt ggcggccgcc ctgcgcgctc
     1561 gtgaactcga agtccatgtc gtcgcgccgg acaagcggcc gatggaacga attctaggcc
     1621 cgcagatggg cgacttcgtg cgcgcgctcc acgaggaaca cggtgtcgtt ttccacctcg
     1681 aggagacggc gggcgccatc gacggcaagc aggtgaagct caaaggcgga ggcacgctcg
     1741 aggcggactt cgtggtcgcc ggtatcggcg tacgcccgcg aatcgagctc gccgaaaggg
     1801 ccgggctcgc cgtcgatcgc ggtgtcgtcg taaatgccta tctcgaaacg agcgctcccg
     1861 ggttcttcgc ggccggcgac attgcacgct ggcccgatcc ccacagcggc gagagcattc
     1921 gcgtcgagca ttgggtggtt gccgagcgcc aggggcaaac agcggcgcgt aacatgctcg
     1981 ggcaccgcga gaaatttacc gccgtcccct tcttctggag ccagcactac gacattccga
     2041 tcaactatgt cggccatgcc gagaaatggg acgaactcgc gatcgagggc gacatcgccg
     2101 gcaaggactg cctcctgcgc ttcaggcgcg ataggcgcac gctcgccgtc gcctcgatct
     2161 tccgcgacat cgaaagcctc cgggccgaag cagcaatgga gcgggaggtg gcgctttagc
     2221 gggtttcctt ggccggcgta gtgcgcaaga tcgacctggg cgaaggcatg tgctttgcgt
     2281 tttcacgcac aaaatcgtcg tggtgattcc cgcctgagcc cgatcgggca acccttcggc
     2341 cgatcagaag ggcggagcgc cccgcgccga ttccatgaac gcaaagcccg ggaaaacaga
     2401 tttgtttgtg gtcaatttta tacagacggc ccgcgatcat ggcgcctatc ctgacaacga
     2461 gccggtgccg tcctctgccc tgacgagatc gcgcccccga agctccggtt ctggcaggct
     2521 gg
//
LOCUS       DASPXW010000034         2960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_262163, whole genome shotgun sequence.
ACCESSION   DASPXW010000034 DASPXW010000000
VERSION     DASPXW010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262163"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VEL04_00725"
     CDS             complement(<1..516)
                     /locus_tag="VEL04_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSHA biogenesis protein MshE"
                     /protein_id="HYT46917.1"
                     /translation="MGRPEKIRLGDTLIAQKIISQEQLKIGLEQQKKSGRRLGRVLIE
                     LGFTNDEQICEAISRQLNVPYVNLKFYNFNNDLVRRLPEAQARRFRAVVLEERRNAYL
                     VGMADPTDLFAFDELARLLKRDVETAVVSEPMLLQNIDRIYRHTDQISGLAKELERDL
                     GDAYIDFGALGA"
     gene            736..1689
                     /locus_tag="VEL04_00730"
     CDS             736..1689
                     /locus_tag="VEL04_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HYT46918.1"
                     /translation="MADAFQEMAVFARVVAAGSLSAAARELGLSPALISRRLAGLEAR
                     LGVRLINRTTRSLYLTDEGASYHETCARLLAEIGEADAAVSAGSVEPQGSLRVALPAS
                     FGQQHVAPLIPDFAARHPKVQLALSLSDRNVNVIEEGFDLAVRIAELEDSSLAARKLA
                     PNRRVVCASPAYLALHGAPRLPEDLARHNCLTTSDFAMNWEYKDPRGKRASVRVAGRY
                     ACDNWSVLREWALAGLGVALKSTWDVYRHLRDGSLVALFPGYSFHSDVAIYAVYPHRR
                     HLPAKTRVFIEFLAASLGPEPYWDQPGARSPKPRIKPRATV"
     gene            1712..>2960
                     /locus_tag="VEL04_00735"
     CDS             1712..>2960
                     /locus_tag="VEL04_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HYT46919.1"
                     /translation="MNAPADRAVLAAASLEDKYTLERGRVFMTGTQALIRLLMLQRER
                     DRAAGLNTAGFVSGYRGSPLGGLDQALWRAQKHLESHHIRFQPGVNEDLAAASIWGTQ
                     QVNLFAGAKYDGVFAMWYGKGPGVDRCGDVFKHANAAGTAQHGGVLVLAGDDHAAKSS
                     TLPHQSEHIFKACLIPVFNPSSVQDYLDLGLHGIAMSRYSGCWVAFKCVTDVVESGAS
                     VVVDANRINVKIPQDFALPGGGLNIRWPDGILEQEARILDWKVYAALAYVRANGLDQI
                     VWDAPRARLGIVTTGKSFGDVMQALADLGIDERVARDIGLRVYKVAMSWPLEPQGARR
                     FAEGLEEILVVEEKRQVIEYQIKEELYNWKEGVRAPRVVGKFDDSGEWSRSSGQPAGT
                     WLLPAHYEHSPAMVARAIAQRLSK"
BASE COUNT          514 a          972 c          972 g          502 t
ORIGIN      
        1 ggcgccgagc gccccgaagt cgatgtacgc atcgccgaga tcgcgctcga gctccttggc
       61 gaggccgctg atttggtcgg tgtggcggta tatccggtcg atgttctgca gcagcatcgg
      121 ctcgctcacc acggcggtct cgacatcgcg cttgagcagc cgggcgagct cgtcgaacgc
      181 gaacagatcc gtcggatccg ccatgccgac gagataagcg ttgcgccttt cttccagcac
      241 cacggcgcgg aagcggcgcg cctgggcttc cggcaggcgc cgcaccaggt cgttgttgaa
      301 gttgtagaac ttgaggttga cgtacggcac gttgagctgg cgcgagatcg cctcgcagat
      361 ctgctcgtcg ttggtgaagc ccagctcgat cagcacgcgc ccgagccggc ggccggactt
      421 tttctgctgc tcgagcccga tcttcagctg ctcctgcgaa atgatcttct gcgcgatcag
      481 cgtgtcgccc agccggatct tctcgggacg ccccatcggt ttctccctct gcaggcgatt
      541 atcgcggatg gacccgaagg cgatcgcctg aaaaaataag gggcagaacc ctttcgggcc
      601 cgccccttag cgcaacccgg cggaaagagg aggaggagga tccgcccggg ctgctgcaac
      661 gcacaaatcc tacgtctttt acccgagggt tagaatacta cacgtttgta aaacactttt
      721 gcatattttg caaagatggc ggatgcattt caagagatgg cagtatttgc gcgggtagtg
      781 gccgcaggaa gcctgtcggc cgccgcccgc gagcttggcc tttcgcccgc gctgatcagc
      841 cggcggctcg ccggcctcga ggcgaggctc ggcgtgcgcc tcatcaatcg gactacccgc
      901 agcctctacc tcaccgatga gggcgcgagc tatcacgaga cctgcgcgcg cctgctggcc
      961 gagatcgggg aggccgatgc cgcggtgtcc gctggaagtg tcgagccaca gggatcgctg
     1021 agggttgcgc tgccggcttc gttcgggcag cagcacgtcg cgccgctgat cccggatttc
     1081 gccgcgcgcc atccgaaggt ccagcttgcg ctcagcctgt cggatcgcaa cgtcaacgtg
     1141 atcgaggagg gattcgacct cgcggtgcgc atcgccgagc tcgaggactc ctcccttgcg
     1201 gcgcgcaagc tggcgccgaa ccggcgggtg gtgtgcgcga gccccgccta cctcgccctg
     1261 cacggcgcgc cgcggctgcc cgaggatctc gcccgccaca attgcctcac gaccagcgac
     1321 tttgcgatga actgggagta caaagacccc cgcggcaagc gcgcatcggt gcgcgtggcg
     1381 ggacgctatg catgcgataa ctggagcgtg ctgcgcgaat gggcgctcgc cggcctcggt
     1441 gtcgcgctga aatcgacctg ggatgtgtac cgccacctgc gggacggctc gttggtggcg
     1501 ttattcccgg gttacagctt tcacagcgac gttgcgatct acgccgtcta tccgcaccgc
     1561 cggcatctcc ccgcgaagac gagggtcttt atagagttcc tcgccgcgtc gctcggaccc
     1621 gaaccttatt gggaccagcc cggcgcgcgc tcgccgaagc ccagaatcaa accgcgcgcc
     1681 acggtctaac tcttatacta aaagagcctc gatgaacgcc ccagccgacc gcgccgtcct
     1741 cgccgcagcc tcgctcgaag acaagtacac gctcgagcgc ggccgggtat tcatgaccgg
     1801 aacgcaggcg ctgatccgcc tgctgatgct gcagcgcgag cgcgaccgcg cagcaggcct
     1861 gaacacggcc ggattcgtct ccggctaccg cggctcgccg ctcggcgggc tcgaccaggc
     1921 gctgtggcgc gcgcaaaagc atctcgagag ccatcacatc cgcttccagc ccggcgtcaa
     1981 cgaggacctg gccgccgcct cgatctgggg cacgcagcag gtgaatctct tcgccggggc
     2041 gaaatacgac ggcgtattcg cgatgtggta cggcaaggga ccaggggtgg atcgctgcgg
     2101 cgatgtcttc aagcacgcca acgccgcagg caccgcgcag cacggcggcg tgctggtgct
     2161 cgcgggcgac gaccatgcgg cgaaatcgtc gacgctgccg caccagtccg agcacatctt
     2221 caaggcctgc ctgatcccgg tgttcaatcc gtcgagcgtg caggactacc tcgacctcgg
     2281 gctgcacggc atcgcgatga gccgctactc gggctgctgg gtggcgttca aatgcgtcac
     2341 cgacgtggtc gagtcaggcg cgtccgtagt agtggatgca aaccgaatca acgtaaaaat
     2401 accgcaggac ttcgcgcttc ccgggggcgg cctcaacatc cgctggcccg acggcatcct
     2461 cgagcaggaa gcgcgcatcc tcgactggaa ggtatacgcc gccctggcgt acgtgcgcgc
     2521 gaacggcctc gaccagatag tgtgggatgc gccgcgagcg cggctgggta tcgtcaccac
     2581 cggcaagtcg ttcggcgacg tgatgcaagc gctcgccgac ctcggcatcg acgagcgcgt
     2641 cgcgcgcgac atcggcctgc gcgtctacaa ggtggcgatg tcctggccgc tcgagccgca
     2701 gggcgcgcgg cgctttgccg aggggctgga ggagatcctg gtggtcgagg agaaacgcca
     2761 ggtgatcgag taccagatca aggaggagct ctacaactgg aaggaaggcg tgcgcgcgcc
     2821 gcgcgtcgtc ggcaagttcg acgacagcgg cgaatggagc cgctcgagcg gccagcccgc
     2881 cggcacctgg ctgctgcctg cacattacga gcactcgccg gcaatggtgg cgcgcgcgat
     2941 tgcgcagcgc ctttcgaaat
//
LOCUS       DASPXW010000035         8090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1776177, whole genome shotgun sequence.
ACCESSION   DASPXW010000035 DASPXW010000000
VERSION     DASPXW010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8090
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1776177"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VEL04_00740"
     CDS             complement(<1..193)
                     /locus_tag="VEL04_00740"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYT46920.1"
                     /translation="MPERALNRICYVEDDEDIQRIVRMSLERIGKMTVEVVGDPTVAI
                     EKMAVFRPDLVMLDWMMPVM"
     gene            complement(207..2003)
                     /locus_tag="VEL04_00745"
     CDS             complement(207..2003)
                     /locus_tag="VEL04_00745"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HYT46921.1"
                     /translation="MNHTGTANGAAIRASEQGVAAGIRDRADPRLVLADRVEQLYSQV
                     PLGVVATLVTGLIAAIELREGRFIELVFFWSALVVLVSVLHALLYHQYRRNAANRDQA
                     EQWMRWLGIGAFAAGAIWGFAAAVFFPSHADERQVFLAFLLAGVLSGGIPMYAASWPM
                     FAAYAAGIALPFTYVLATFGNRLFAEIALLIPLFYGVSVAIAYRLNRVFDSGYRLRHA
                     YGRLTEDYSGLNQRLEQQLVELEEARRQVEASGRKLALFAERAPIAVLELDPDGIVKE
                     VNHAAELLFGYAAAELVGGHVKRLVGPKYHADYDRDWAKLIATREPVAGVTVRNPRRD
                     GIDLVCEWTVTPLVNPEGKLLAVIAQGRDITAQREAERMKKEFTSTLSHELRTPLTSI
                     IGSLQLINSGVLGDIVPDVAELTAIAERNGQRLLDLINDILDIEKIESGKLILSPEVM
                     RVDELVSEALLLNKAFGERFKVRFQSHGDLPARTVNADRKRLQQVMTNLLSNAAKFSP
                     ENDVVEVTTEDRGANVRVAIHDRGSGIPEGFRNRIFGRFTQADSTTARQKGGTGLGLA
                     ICKRLVEMMQGHIGFEDRPGGGTSFWFELPRH"
     gene            complement(2066..2695)
                     /gene="lexA"
                     /locus_tag="VEL04_00750"
     CDS             complement(2066..2695)
                     /gene="lexA"
                     /locus_tag="VEL04_00750"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HYT46922.1"
                     /translation="MDELTARQTEILRLVRELTEVSGYPPTRAEIAERMGFRSVNAAE
                     QHLRALERKGAIQIASGASRGIRVRDGRPRGQLSRLLELPVIGRVAAGSPMLAEEHVQ
                     GRYQVDPNLFTPRADYLLRVRGMSMRDAGILEGDLLAVHRTQEARSGQIVVARLTDEV
                     TVKRWRRRGHMVQLVPENPEFQPIEVDLRSETLAIEGIAVGVIRNGRSL"
     gene            complement(2727..3347)
                     /locus_tag="VEL04_00755"
     CDS             complement(2727..3347)
                     /locus_tag="VEL04_00755"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HYT46923.1"
                     /translation="MCGRYALHSHPEVVALQFGLDVTPRFDARYNICPSTQILVVRTN
                     GERKRVADTYRWGLGNRLANARGETLAQTPAFRNAFRYSRCLVPASGFYEWQTLAGKK
                     QPWYMRPKDAAVFGLAAIAELWNGVRSVALITTRANQLMQSIHDRMPVIVLPENYGTW
                     LDRGNGNISELQNLIRPYASERMQAYPVSLRVNTPQHDDAALIERI"
     gene            3374..4558
                     /gene="pncB"
                     /locus_tag="VEL04_00760"
     CDS             3374..4558
                     /gene="pncB"
                     /locus_tag="VEL04_00760"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006476526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0019358 - nicotinate nucleotide salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HYT46924.1"
                     /translation="MIIQSLLDTDLYKFTMMQVVLHHFPGAQVEYRFRCRTEGVDLRP
                     HLDDIRGAIEGLCALRFGDDELAYLRGLRFMKSDFVDFLALFQFNQKYLRIGVGEGAG
                     GIEITIRGPWLHTILYEIPVLAIVSEVYFRRTQPHPDLAEGRRRLKAKIDLVRTVEPE
                     LDFRISDFGTRRRFSLAWQEEVLQAFKREVPQYFAGTSNVWFAMRNRLTPLGTMAHEY
                     MQACQALGPRLRDSQIFAFDKWAQEYRGDLGIALSDTYGFDAFLRDFDMYFCKLFDGA
                     RHDSGDPFDWGEKMIAHYRRNRIDPRSKTLIFSDQLSVPLAISIARRFHGRTRTAFGI
                     GTNLTNDLGYEPINIVIKMTECNGQPVAKVSDSPGKTVSKDAGYLRYLRQVFGLEPVE
                     KI"
     gene            4594..5760
                     /locus_tag="VEL04_00765"
     CDS             4594..5760
                     /locus_tag="VEL04_00765"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYT46925.1"
                     /translation="MNMFGRGLGDTYAVFLLPLEREFGWSRSELTGVYSIYLLVNGFT
                     APLVGLAFDRLGPRWVYGAGTALLGAAFFLAGGLVTLWQFYLFVGALVGLGVSFNGMV
                     PGSALLVRWYRARLSTAIGVAFSAVGVGTIVFVPLAQYLVAQYDWRVAYRTLGALLLG
                     LLPLVLFAVPWRTFAAGDPLFRPDARQKASGEGWTLRAALGTPVYRGLAQLFFCTAAA
                     MFSIVVQLVAFLIDAGFSPLTAATAYGLVGMMSAASIMGSGLASDRFGYRQTVSVSFM
                     GTASGMVLLLFITGFPSILLLIAFVPVFGLCMGVRGPIVASVAARYFAGPNVATIYGT
                     IYSTNALGAAFGSWMGGLLHDLTDGYRAGLGLALAFIALAAAPFWTVSGLRNFR"
     gene            complement(5735..6892)
                     /locus_tag="VEL04_00770"
     CDS             complement(5735..6892)
                     /locus_tag="VEL04_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HYT46926.1"
                     /translation="MVDLGSVIRPGDGIIWGQACAEPQTLVEALVAQRASFPGCSVFL
                     GSSYSGLVRPEHADHLRLSSYCGTGTNRALAEAGVLDINPAPYSQLGLLIEARKIKCD
                     VVMLQVSPPNGRGEYSLGLGVEFLAPALQAARAVIAEVNDQVPWTHTEPLLRSNDFAL
                     LVHSSRPPVSLPYRLDELELAIAGNAAEFIPERAVLECGIGNLPNAVLAALSGHRDLR
                     FHSGVIPDGVVPLARAGVLKSVLGGALMGTRQLFDWARENPAVQLRSSTYTHGAATLA
                     KIERFVAINTAVEVDFTGQVNGELARGSYVGAVGGALDFMRAANQSPGGVSITALPSS
                     RIVAKLSGPVSVPRSELGVVVTEKGAADLRGCSLREREQRMRAISGNFSAR"
     gene            6903..7484
                     /locus_tag="VEL04_00775"
     CDS             6903..7484
                     /locus_tag="VEL04_00775"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HYT46927.1"
                     /translation="MTDERTLDLLLLKARTHNDFQDQPVPEDLLRKAHDIMKWGPTSA
                     NSQPARLVYLRSRESREKLRPALSATNLEKTMKAPLAAIVAYDTRFWEHLPRMFHNPE
                     AINWFRDKGAHTEVTAFRNGTLQGGYYLLALRAVGLDCGAMSGFDNAKVDAAFFPDGR
                     LKSNFLVNIGYGSGKNMAPRNPRLTFEEQCRIL"
     gene            complement(7453..7821)
                     /locus_tag="VEL04_00780"
     CDS             complement(7453..7821)
                     /locus_tag="VEL04_00780"
                     /inference="COORDINATES: protein motif:HMM:NF024515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HYT46928.1"
                     /translation="MAKNALFLIALLVAAPAQAQRLQAALECKFAGTDFIYDCIIRLT
                     PPLSGARITVGAEMPSMPMAHNIKPVKAAPGKAPGEYRARLDLEMRGEWAVKLRISGP
                     LREQLILHYDFTESGTAPRK"
     gene            complement(7797..>8090)
                     /locus_tag="VEL04_00785"
     CDS             complement(7797..>8090)
                     /locus_tag="VEL04_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYT46929.1"
                     /translation="AAIGGCLYAHLNTYIEPRNFDIMLGVHSLAYALIGGLGSILGPL
                     LGVALDLGLLEGTRFLHGYRMIVFGGLVALLLVFRPRGLLDERVTHWLRTRFS"
BASE COUNT         1287 a         2680 c         2693 g         1430 t
ORIGIN      
        1 ccatcaccgg catcatccag tcgagcatca ccagatcggg ccggaacacg gccatcttct
       61 cgatcgccac ggtgggatcg cccaccacct ccacggtcat cttgccgatg cgctcgagcg
      121 acatgcgcac gatgcgctgg atgtcctcgt cgtcctcgac gtagcagatg cggttgagcg
      181 ctcgttcggg catcgcgtgc tcctggtcag tggcgcggca gctcgaacca gaagctggtg
      241 ccgccgcccg ggcggtcctc gaagccgatg tgcccctgca tcatctctac cagccgcttg
      301 cagatggcga ggccgaggcc ggtgccgccc ttctggcgcg cggtggtcga gtcggcctgc
      361 gtgaagcggc cgaagatgcg gttgcggaag ccttccggaa tgccgctgcc gcggtcgtgg
      421 atcgcaacgc gcacgttggc gccgcggtcc tcggtggtga cctcgacgac gtcgttttcg
      481 ggggagaact tggcggcatt cgacagcagg ttggtcatca cctgctgcag ccgcttgcgg
      541 tcggcgttca ccgtgcgcgc tggaagatcg ccgtgcgact ggaagcgcac cttgaagcgc
      601 tcgccgaagg ccttgttgag cagcagcgcc tcgctcacca gctcgtcgac gcgcatcact
      661 tcgggcgaga ggatcagctt gccggactcg atcttctcga tgtcgaggat gtcgttgatc
      721 aggtcgagca gccgctggcc gttgcgctcc gcgatcgcgg tcagctcggc gacgtcgggc
      781 acgatgtcgc cgagcacgcc ggaattgatg agctggagcg agccgatgat cgaggtgagc
      841 ggcgtgcgca gctcatggct gagcgtcgag gtgaattcct tcttcatgcg ctcggcttcg
      901 cgctgggcgg tgatatcgcg gccctgcgcg atcacggcga gcagcttgcc ttcggggttg
      961 acgagcgggg tcacggtcca ttcgcacacc agatcgatgc cgtcgcggcg cgggttgcgc
     1021 acggtgacgc cggcgaccgg ctcgcgggtg gcgatcagct tggcccagtc gcggtcgtag
     1081 tcggcgtggt atttcggccc caccagccgc ttcacgtggc cgccgacgag ctccgcggcc
     1141 gcgtaaccga acagcagctc ggcggcgtga ttgacttcct ttacgatgcc gtcggggtcc
     1201 agctcgagca ccgcgatcgg cgcgcgctcg gcgaacagcg cgagcttgcg tccgctcgcc
     1261 tcgacctggc ggcgcgcctc ttcgagctcg accagctgct gctccagccg ctggttgagc
     1321 ccgctgtaat cctcggtcag ccgcccgtag gcgtggcgca ggcggtagcc tgaatcgaac
     1381 acgcggttga gccggtacgc gatcgccacg ctcacgccgt agaacagcgg aatcagcagc
     1441 gcgatctcgg cgaacaggcg gttgccgaac gtcgccagca cgtaggtgaa cggcagcgcg
     1501 atgccggcgg cgtatgcggc gaacatcggc cacgaggccg cgtacatcgg gatgccgccc
     1561 gacagcacgc ccgccagaag gaaggcgagg aacacctggc gctcgtcggc gtgcgagggg
     1621 aaaaacaccg cggcggcgaa tccccagatc gcgcccgcgg cgaaggcgcc gatgccgagc
     1681 cagcgcatcc attgctcggc ctggtcgcgg ttcgcggcat tgcgccggta ctggtggtag
     1741 aggagggcgt gcagcacgct gaccagcacc accagcgcgc tccagaagaa caccagctcg
     1801 atgaagcggc cttcgcgcag ctcgatggcg gcgatcagcc cggttaccag cgtcgcgacc
     1861 acgccgagcg gcacctggct gtagagctgc tccacgcgat cggcgagcac cagtcgcgga
     1921 tcggcccggt cgcggattcc cgctgcaacg ccctgttcgg acgcccggat cgcggcgccg
     1981 tttgcagtgc cggtgtgatt catctgcgca ggcattctcc ggcgccaatt ctagcgggaa
     2041 agaccggact gactgacagg agttgctaaa ggctgcggcc gttgcggatc acgccaaccg
     2101 caatgccttc gatggcgagt gtctcgctgc gcaggtcgac ttcgatcggc tggaactcgg
     2161 ggttctcggg gaccagctgc accatgtggc cgcggcggcg ccagcgcttg accgtcacct
     2221 catcggtgag ccgggccacc acgatctggc cgctgcgtgc ctcttgcgtg cgatgcacag
     2281 cgagcaggtc gccctcgagg atgccggcat cgcgcatcga catgccgcgc acccgcagca
     2341 gatagtcggc gcgcggcgtg aacaggttcg gatccacctg gtagcgcccc tgcacgtgct
     2401 cttcggcgag catcgggctt cccgcggcga cccggccgat gaccggcagc tcgagcaggc
     2461 gcgagagctg tccgcgcggg cggccgtcgc gcacgcggat gccgcgcgag gcgcccgacg
     2521 cgatctggat cgcgcccttg cgctctagcg cgcgcaggtg ctgctcggcg gcgttcaccg
     2581 agcgaaaacc cattctttcg gcgatctcgg cgcgcgtcgg cggataaccc gaaacttcgg
     2641 tcagctcgcg caccaggcgc aggatctcgg tctgtcgggc agtgagctca tccatacatg
     2701 gcattatatc cagtatgggc ggggcgtcaa atgcgctcga tcagcgcggc gtcgtcgtgc
     2761 tggggcgtat tcacccgcag gctgaccgga taggcctgca tgcgctcgga ggcgtagggc
     2821 cggatcaagt tttgtaactc tgagatattg ccgtttccac ggtcgagcca agtgccatag
     2881 ttctcgggca aaacgatgac cggcatgcgg tcgtggatgc tctgcatgag ctggttcgct
     2941 cgtgtggtga tcagggcgac gctgcgcacg ccgttccaca gctcggcgat tgccgcgagg
     3001 ccgaagaccg cggcgtcctt cgggcgcatg taccacggct gctttttccc ggccagcgtc
     3061 tgccattcgt agaagccgct cgccggcacc aggcagcgcg agtagcggaa ggcgttgcgg
     3121 aacgccggcg tctgcgccag cgtctcgccg cgcgcgttgg cgagcctgtt gcccagcccc
     3181 cagcggtaag tatccgcaac ccgctttcgc tcgccgttgg ttctgacaac gagaatctgc
     3241 gtgctcgggc agatgttgta gcgggcgtcg aaacgcggag tgacatcgag gccgaactgc
     3301 agcgcgacga cctcgggatg gctgtggagc gcgtaacggc cgcacatcgg ccgaaggtta
     3361 caatgcctcc ggcatgatca tccagtcgct gctcgacacc gacctgtaca agttcaccat
     3421 gatgcaggtg gtgctgcatc attttcccgg cgcgcaggtc gagtaccgct tccgctgccg
     3481 caccgagggc gtcgatctgc ggccgcacct cgacgacatc cgcggcgcga tcgaaggtct
     3541 ctgcgcgctg cgcttcggcg acgacgagct cgcctacttg cgcggcctgc gcttcatgaa
     3601 atcggacttc gtcgactttc tggcgctgtt ccagttcaac cagaagtacc tgcgcatcgg
     3661 cgtgggcgag ggcgcgggcg gcatcgaaat cacgattcgc ggtccctggc tgcacaccat
     3721 cctgtacgag atcccggtgc tcgcgatcgt ctccgaggtg tatttccgcc gcacgcagcc
     3781 gcatcccgat ctggccgagg gcaggcggcg gctcaaggca aagatcgacc tggtgcgcac
     3841 cgtcgagccc gagctcgact tcaggatctc ggatttcggc acgcgccggc gcttctcgct
     3901 cgcctggcag gaggaggtgc tgcaggcctt caagcgggag gtgccgcagt atttcgccgg
     3961 cacgtcgaac gtctggttcg cgatgcgcaa ccgcctcacg ccgctcggca ccatggcgca
     4021 cgagtacatg caggcctgcc aggcgctcgg gccgcggctg cgcgattcgc agattttcgc
     4081 gttcgacaag tgggcgcagg agtatcgcgg cgacctcggc atcgcgctct ccgataccta
     4141 cggcttcgac gccttcctgc gcgatttcga catgtacttc tgcaagctgt ttgacggcgc
     4201 gcgccacgac tcgggcgacc cgttcgactg gggcgagaag atgatcgcgc attaccgccg
     4261 caaccgcatc gatccgcgct cgaagacgct gatcttttcc gaccagctct cggtgccgct
     4321 cgccatcagc atcgcgcggc gctttcatgg ccggacgcgc accgccttcg gcatcggcac
     4381 caacctcacc aacgacctcg gctacgagcc gatcaacatc gtcatcaaga tgaccgagtg
     4441 caacggccag ccggtggcga aggtctcgga ctcgcccggc aagacggtga gcaaggacgc
     4501 cggatacctg cgttatctgc gccaggtttt tgggctggag ccggtcgaaa agatctgaaa
     4561 acagccactg cagtcctcgc ggcctgcttc ctgctgaaca tgttcgggcg ggggctgggc
     4621 gacacttacg ccgtgttcct gctgccgctc gagcgcgagt tcggctggtc gcgctccgag
     4681 ctcaccggcg tgtactcgat ctacctgctg gtgaacggct tcaccgcgcc gctggtcggc
     4741 ctcgccttcg accggctcgg cccgcgctgg gtttacggcg ccggcacagc cctgctcggc
     4801 gccgcctttt tcctcgccgg ggggctggtg accctgtggc agttctacct tttcgtaggt
     4861 gccctggtgg gattgggcgt cagcttcaac ggcatggtgc cggggtccgc gctgctcgtg
     4921 cgctggtatc gcgcgcggct gtcgactgcg atcggcgtcg cgttctccgc agtcggcgtt
     4981 ggaacgatcg tgttcgtgcc cctcgcgcag tacctggtcg cccagtacga ctggcgcgtg
     5041 gcgtaccgga ctctcggcgc gctgctgctc gggctgctgc cgctggtcct gttcgcggtg
     5101 ccatggagga ctttcgccgc cggcgatcct ctgttccggc cggatgccag gcaaaaagcg
     5161 tcaggcgaag gctggacgct gcgcgccgcg ctcggcacgc cggtctaccg gggcctggcg
     5221 cagctcttct tctgcaccgc ggctgcgatg ttctcgatcg tcgtccagct ggtggctttc
     5281 ctgatcgacg caggcttttc gccgctcacc gcggccaccg cttacggcct ggtcggcatg
     5341 atgtcggcgg cgagcatcat gggcagcggg cttgcctccg accgcttcgg ctaccgccag
     5401 accgtgagcg tcagtttcat gggtacggcc agcggaatgg ttctgcttct attcatcacc
     5461 ggatttcctt caattctgct gctgatcgca ttcgtgccgg tattcggcct gtgcatgggg
     5521 gtgcgcgggc cgatcgtcgc ctcggtcgcg gcgcgctact ttgccgggcc caacgtggcc
     5581 acgatctacg gcacgatcta ttcgaccaac gcgctcggcg ccgcgttcgg ctcgtggatg
     5641 ggagggctgc tgcacgacct caccgacggc tatcgcgccg gcctcggcct ggcgctcgct
     5701 ttcatcgcgc tcgccgcggc gccgttctgg accgtcagcg ggctgagaaa tttccgctga
     5761 tcgcccgcat gcgctgctct ctttcgcgca gcgagcagcc gcgaagatcc gcggcgcctt
     5821 tctcggtgac caccaccccg agctcgctgc gcggcacgga cactggcccg gacagctttg
     5881 cgacgatcct tgatgaaggt agggccgtga tggaaacgcc gccgggcgac tggttggcgg
     5941 cgcgcatgaa gtcgagcgcg ccgcccaccg cgccgacata gctgccgcgg gcgagctcgc
     6001 cgttgacctg gccggtgaag tcgacttcca cggccgtatt gatggcgacg aaacgctcga
     6061 tttttgcgag cgtcgcggcg ccgtgcgtgt aggtggaaga gcgcagctgg accgcgggat
     6121 tctcgcgcgc ccagtcgaac agctgtcgcg tgcccatcag cgcgccgcca aggacgcttt
     6181 tcaacacccc ggcgcgcgcg agcggcacga cgccgtccgg aatcacaccc gagtggaagc
     6241 gcaggtcgcg gtgtccggac agggcggcga gcaccgcgtt gggcaggttg cctatgccgc
     6301 attccaagac tgcgcgctcg ggaatgaatt ccgcggcatt cccggcgatg gcgagttcca
     6361 gctcatccag cctgtaaggc agggataccg gcggacgcga gctgtgcacc agcagcgcga
     6421 aatcgttgct gcgcagcagc ggctcggtgt gagtccacgg cacctgatcg ttcacctccg
     6481 cgatcaccgc acgcgccgct tgcagcgcgg gagcgaggaa ctcgacgccc agccccaggc
     6541 tgtactcgcc gcgcccgttc ggcgggctga cctggagcat cacgacgtcg catttgattt
     6601 tccgggcctc gatcagtagt ccgagctggg agtagggcgc cggattgatg tcgagcaccc
     6661 cggcttcggc gagcgcgcgg ttggtcccgg tgccgcagta ggacgagagc ctcaggtgat
     6721 cggcatgctc gggtctgacg aggccggagt agctcgagcc gaggaagacg ctgcagcccg
     6781 ggaatgacgc gcgctgcgcg accagcgctt cgaccagggt ttgcggctcg gcgcaggcct
     6841 ggccccagat gattccgtca cccggacgaa ttacactacc gagatccacc atcgggaaga
     6901 ttatgaccga cgaaagaact ctcgatctgc tgcttctgaa ggcacgcacg cacaacgatt
     6961 tccaggacca gccggtgccc gaagatctgc tgcgcaaggc tcacgacatc atgaaatggg
     7021 gtccgaccag cgccaacagc cagccggcgc gcctggttta tttgcgttcc agagaatcga
     7081 gggaaaagct gcgccccgct ctcagcgcga ccaaccttga gaagaccatg aaggcgccgc
     7141 tggcggcgat cgtcgcctac gacacgcgct tctgggagca cctgccgcgc atgttccaca
     7201 accccgaggc gatcaactgg ttcagggaca agggcgcgca taccgaggtc acggcgtttc
     7261 gcaacggcac gctgcagggt ggctactacc tgcttgcgtt acgcgcagtc ggactggatt
     7321 gcggcgccat gtccggcttc gacaacgcca aggtcgacgc ggcgttcttc cccgacggcc
     7381 gtttgaagtc gaatttcctc gtcaacatcg gctacggctc gggcaagaac atggcgccgc
     7441 gcaacccgcg cctcactttc gaggagcagt gccggattct gtaaagtcgt agtgcaggat
     7501 cagctgctcg cgcagcgggc cggagatcct gagcttcacc gcccattcgc cgcgcatctc
     7561 caggtcgagc cgcgcccggt attccccggg cgccttgccc ggtgcggcct tcaccggctt
     7621 gatgttgtgc gccatcggca tcgatggcat ctcggcgcct accgtgatgc gcgcgccgga
     7681 aagcggtggg gtgagacgaa tgatgcagtc gtaaatgaaa tcagtgccgg cgaatttgca
     7741 ttccagggct gcctgcaggc gctgcgcctg tgccggcgcg gcgaccagca gcgcgatcag
     7801 gaaaagcgcg ttcttagcca atgcgtcacc cgctcgtcga gcagcccgcg cggccggaac
     7861 accagcagca gcgcgaccag gccgccgaac acgatcatgc gatagccatg caggaacctg
     7921 gtgccctcga ggaggccgag gtcgagcgcg acgccgagca gcggacccag gatgcttcca
     7981 agcccgccga tcagcgcgta cgccaggctg tgcacgccga gcatgatgtc gaagttgcgc
     8041 ggctcgatgt aggtgttgag atgcgcgtaa aggcaccccc cgatcgccgc
//
LOCUS       DASPXW010000036         3131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_388673, whole genome shotgun sequence.
ACCESSION   DASPXW010000036 DASPXW010000000
VERSION     DASPXW010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388673"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..165
                     /locus_tag="VEL04_00790"
     CDS             <1..165
                     /locus_tag="VEL04_00790"
                     /inference="COORDINATES: protein motif:HMM:NF021510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 family protein"
                     /protein_id="HYT46930.1"
                     /translation="RGLKHGDRWCLHVLRWVEAWEAGRAPRVVLEATHENVLKYAPLS
                     ALKRHALDLN"
     gene            352..2073
                     /locus_tag="VEL04_00795"
     CDS             352..2073
                     /locus_tag="VEL04_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT46931.1"
                     /translation="MAVALALAGMAAVPVAALADGVNQNCPEESVYYNPSNGEDIAVP
                     QGFRVEAFITGLNFPTDIAFVGGANDFKVYVLESGTGLPGKCNNNVGVYGGPFAPANP
                     FTPDIVIFDRHGHKTGGPLGKPTLAGGGFQPDGPAIGLAFENNFDGGTLYATDSNQGA
                     RGAPGKGNNTSRVLRVSLPAGTITPLIVGLPTGDHPTEQIVAKDGWLYWSQGSATNSG
                     VTGHDNGAGGNQHDIACQQITLSQNVWDSGDGHVTSGYSNHGVARPGAVVPAFEDATS
                     KGMCTGAILRAKISDPQNTIEPVAWGFRNPFGIRFSPVDHPLKGQMMISENGEDERGA
                     RPTNNAPDRLAVARQNPDGTPEWHGWADRFGFLDSTQSVFNPKGSSGDDNPAAIANKP
                     VLPVFAFFPQQPVAPLALEPADVAAVGLDFVPDSFVGGVVKKNAALVSREGDFGFSQA
                     NGTPEAGHDVELVNFTGQTPSELQLSRFAFNCRQREQFQDPDGTPKCSGETEQAFVAR
                     MRGINRPVTIRFGPDGAAYLVDYGAVRDPGGSDPDSRFVNPDNAPLVQIPGTGTIWKI
                     SRIGGGH"
     gene            2154..2765
                     /gene="ccmA"
                     /locus_tag="VEL04_00800"
     CDS             2154..2765
                     /gene="ccmA"
                     /locus_tag="VEL04_00800"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HYT46932.1"
                     /translation="MLEANDLECERGGRTLFSHLSFAVGKGELLRIAGTNGSGKTSLL
                     RILCGLLSPDAGEVRWQGSAILRLREEYSKQLLYLGHAPAVKDDLTPAENLRITCTLA
                     GVAVVEYAIEESLGRFGLSGIDPPVKKLSQGQKRRAALARLLLSESIPLWLLDEPFAA
                     LDASATRLVGELIDGHVARGGAVVFTTHQDTGVGASRVIDLDA"
     gene            2758..>3131
                     /locus_tag="VEL04_00805"
     CDS             2758..>3131
                     /locus_tag="VEL04_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HYT46933.1"
                     /translation="MLDAARCVVVRDLLVAWRRRSDVATALLFFVIVASLFPLGVGAE
                     PNLLRAIAPGVIWVAALLSSMLSLGRLFAADHADGTLEQLLLGAAPLGVIAAAKAFAH
                     WLVSGLPLALIAPLIALQYDLPS"
BASE COUNT          518 a         1019 c         1044 g          550 t
ORIGIN      
        1 cgcggcctca agcacggcga ccgctggtgc ctgcacgtgc tgcgctgggt cgaggcctgg
       61 gaggccggtc gcgcgccgcg cgtggtgctc gaggctaccc acgaaaacgt gctgaagtac
      121 gcgccgctct cggcgctcaa gcgccatgcg ctcgacttga actgaatgcc gctggatcgc
      181 aggatctgtt gacctgagct tcagcaaagg atcagactga acctgcctca acctttcgag
      241 aggagagatg ttgagcgcgc gcagtcgcag cacaccataa atcagcgaac agttgaacca
      301 gtgggagagg atatggatcc attctttgca cccaagcaga aactgtcttg catggctgtc
      361 gcgctggctc ttgccggcat ggcggctgtg cccgtcgcgg ccctggccga tggcgtgaac
      421 cagaattgcc ctgaggagag cgtgtactac aacccgtcga acggcgaaga catcgccgtg
      481 ccgcagggct tcagggtgga agccttcatc accggactga atttccccac cgatatcgct
      541 ttcgtcggcg gcgcgaacga tttcaaggtg tacgtgctgg agtccggcac cggcttgccg
      601 ggcaagtgca ataacaatgt gggcgtgtac ggcgggccgt tcgccccggc caatcccttt
      661 acgcccgaca tcgtgatctt cgaccggcac gggcacaaga ccggcggccc cctgggcaag
      721 cccacgcttg caggcggcgg cttccagccc gacggtccgg cgatcgggct tgcgttcgag
      781 aacaatttcg atggcggaac gctgtacgcc accgactcca atcagggtgc gcgcggcgcg
      841 ccaggcaagg gcaacaacac gtcgcgcgtc ctgcgcgtca gcctgcctgc gggaacgatt
      901 acgccgctca tcgtcgggct gccgaccggc gatcacccca ccgagcagat cgtggccaag
      961 gacggctggc tctactggtc gcagggctcc gccacgaatt ccggcgtcac cggccatgac
     1021 aacggcgccg gcggaaacca gcacgacatc gcctgccagc agatcacgct cagccagaac
     1081 gtctgggatt cgggcgacgg ccacgtcacg agcggctatt ccaaccacgg cgtggcccgg
     1141 cccggcgcgg tggtgccggc attcgaagac gcgaccagca agggcatgtg caccggcgcc
     1201 atcttgcgcg cgaagatttc ggatccgcag aacaccatcg agccggtcgc ctggggattc
     1261 cgcaatccgt tcggcatacg gttttctccg gtcgatcatc cgctcaaggg ccagatgatg
     1321 atttccgaga acggcgagga tgagcgcggc gcccggccga ccaacaatgc gcccgaccgg
     1381 cttgcggtcg cgcgccagaa ccccgacggc acgccggaat ggcatggctg ggccgaccgc
     1441 ttcggtttcc tcgattcgac gcagtcggtc ttcaatccca agggcagctc gggcgatgac
     1501 aatccggcgg cgatcgcaaa caagccggtg cttccggtgt ttgccttttt cccgcagcag
     1561 ccggtggcgc ctctcgcgct cgagcccgcc gacgtcgcgg cagtcgggct ggacttcgtg
     1621 cccgactcgt tcgtcggcgg cgtggtgaag aaaaatgcgg cgctcgtgtc gcgcgagggc
     1681 gatttcggct tctcgcaggc aaacggcacg cccgaagccg gccatgacgt cgagctggtc
     1741 aacttcacgg gccagacgcc gagcgagttg cagctttccc gcttcgcctt caattgcagg
     1801 cagcgcgagc agttccagga ccccgacggc acgccgaaat gctcgggcga aaccgagcag
     1861 gcgtttgtcg cgaggatgcg tggcatcaat cgcccggtca ccattcgctt cgggccggac
     1921 ggcgccgcct acctggtgga ctacggcgcg gtgcgcgatc cgggcggttc cgaccccgac
     1981 tcgcgcttcg tcaatccgga caatgcgccg ctggtgcaga ttccgggcac cggaaccatc
     2041 tggaagatct cgcgcatcgg gggcggccat taagtagaag agcgcggcgg catcacggtg
     2101 cccgcaacgc gagttgcggg catttttttt cccgcctctg caagaatagc ggtgtgctgg
     2161 aagcgaatga cctggagtgc gagcgcggcg gacgcacgct gttcagccat ctttcgttcg
     2221 cagtcggcaa gggcgagctg ctgcgcatcg ccggcacgaa cggcagtggc aagaccagcc
     2281 tgctcaggat cctgtgcggg ctgctgtcgc cggacgcggg cgaagtgcgc tggcaaggaa
     2341 gcgcaatctt gcggctgcgc gaggagtact cgaagcagct gctttacctc gggcatgcgc
     2401 cggcggtaaa ggatgacctc acgccggccg agaatctgcg catcacgtgc acgctcgccg
     2461 gcgtggctgt ggttgaatat gccattgaag aatccctggg gcggttcggc ctgtcaggca
     2521 tcgatccgcc tgtaaaaaag ctttctcagg gccagaagcg gcgggccgcg ctggccaggc
     2581 tgctgttgag cgagtcgatc cccctgtggc tgctcgacga gccgtttgcc gcgctcgacg
     2641 cgtccgcgac ccggctggtc ggggagctga tcgacggcca cgtggcgcgc ggcggggccg
     2701 tggtgttcac gacgcaccag gacaccggtg tcggcgcctc gcgggtcatc gatttagatg
     2761 cttgatgcgg cgcgctgcgt tgtggttcgc gacctgctcg tcgcgtggcg gcgccgcagc
     2821 gatgtcgcca ccgcgctgct cttcttcgtc atcgtggcga gtctttttcc gctcggcgtc
     2881 ggcgccgagc cgaacctgct gcgcgcgatc gctcccgggg tgatctgggt ggcggcgctg
     2941 ctctcctcga tgctttcgct gggtcgtctg ttcgccgccg atcacgccga cggcacgctc
     3001 gaacagctgc tgcttggcgc ggcgccgctc ggcgtgatcg cagccgcgaa ggcctttgcc
     3061 cactggctcg tgtcgggatt gccgcttgcg ctgatagcgc cgctgatcgc gctgcagtac
     3121 gacctgccct c
//
LOCUS       DASPXW010000037         4507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1650573, whole genome shotgun sequence.
ACCESSION   DASPXW010000037 DASPXW010000000
VERSION     DASPXW010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1650573"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VEL04_00810"
     CDS             <1..580
                     /locus_tag="VEL04_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HYT46934.1"
                     /translation="AYANWKDGERASAIAAADRFIKLYPNHPNVDYAWYLKGLINFNE
                     NLSVLWWLTTPDLSDRDPRGSRDAFDAFKEVVQRFPESKYAADSAARMRYLVNALASY
                     EVHVARYYMRRGAYLAAANRAQYAIKSYPQAPALEEAVFVLVKAYDALDMGELRDAAA
                     RVMRVNFPESRYLKVGDVRRDAPWWRIWDPDW"
     gene            550..1563
                     /gene="denD"
                     /locus_tag="VEL04_00815"
     CDS             550..1563
                     /gene="denD"
                     /locus_tag="VEL04_00815"
                     /EC_number="1.1.1.410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011659522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-erythronate dehydrogenase"
                     /protein_id="HYT46935.1"
                     /translation="MVADLGSGLVKPGARRILVTGAAGMIGRKLTQRLVREGAGELIL
                     HDVVPFGENTVVSDLATPGEAEKLLERRPDLIFHLAAIVSGEAEADFEKGYRVNLDGT
                     RRLFEAIRKIQNGYKPRLVFTSSIAVFGAPFPEAIGDEFLSAPLTSYGTQKAISELLL
                     ADCSRRGFFDGIGIRLPTICVRPGKPNLAASGFFSGIIREPLNGEEAMLPVPETVRHW
                     FASPRAAIGFLIHASKINTEHLGARRNLSMPGVAATVAEQIEALRRVAGERVARLIRR
                     RPDPAILRIVEGWPRNFDAQRALALGFRADTSFEEIIRIHIQDEMQTDELPGTPRPRI
                     GSA"
     gene            complement(1484..3436)
                     /locus_tag="VEL04_00820"
     CDS             complement(1484..3436)
                     /locus_tag="VEL04_00820"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HYT46936.1"
                     /translation="MSKRALQRIFAPDGPLAAAVPGFKLRPQQVQMAEAIHEAIERLG
                     VLVAEAGTGTGKTFAYLVPALLAGGKVVVSTGTKTLQDQLFDRDLPAVREALGSGASA
                     ALLKGRANYVCRYRLRRATAEARFATREEASQLQRIERFAAVSTTGDRADLADVPEDA
                     AVWTHATSTRDNCLGQACPDYSECFVMQARRNALAADVVVINHHLFFADVVLRDEGIA
                     ELLPACNTLVFDEAHQLPETARLFFGDTVSSAQLVELSRDARLELRAAGGASPELDRL
                     AASLERAARDLRLSLGEAGARLGWNQAMRLAEFPDAIEGLNASLKKLQAPLETQSERS
                     EGLASCARRAGAALALLVRLRESEARDEVRWVEVFGGAARLHLTPLSPAELFCRQMSD
                     HPRAWIFTSATLAVGDDFAHFTRELGIPQAAARRWASPFDFGRQALLYLPQGLPADPN
                     DAAFTDAVIEAAAPVLEASAGRAFLLFTTLRALRRAHELLRGRLRFPLLVQGTGSRSE
                     LLERFRALGNAVLLGSQSFWEGVDVRGEALSLVVIDKLPFAPPDDPVLAARIDAIRVQ
                     GGNPFTELQLPQAVLQLKQGAGRLIRDETDRGVLMLCDPRLASRAYGRQILRSLPAMK
                     LTRNLSEVEGFLAAHPSASHPECESG"
     gene            complement(3455..4195)
                     /locus_tag="VEL04_00825"
     CDS             complement(3455..4195)
                     /locus_tag="VEL04_00825"
                     /inference="COORDINATES: protein motif:HMM:NF015891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HYT46937.1"
                     /translation="MKLLRSTLAVAAACAASLSAAQHALEIIPLRHRTVEQVLPTLRP
                     LVEPGGTLTGQGNQLIVRASPANLADIRRALDAIDRPLRRLQISVRFDDALEASSEGV
                     EASGRISNRGSRLEVQARQSSSRADERVDQRVQVLEGGRAMIFTGQSRPVVAREVVAV
                     QDLATGFEVVPRISGENVYLELAPQRETPQSFERASSSVTGRLGEWFELGAVGSSATR
                     ADRGIASASGARALQSRRIWVKVEEVRN"
     gene            complement(4318..>4507)
                     /locus_tag="VEL04_00830"
     CDS             complement(4318..>4507)
                     /locus_tag="VEL04_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT46938.1"
                     /translation="IGVAVAANGAAALNLKASSGYQILDQQALEMFRQATQAVPVPLA
                     LRGKEFAFEVRAVYGLED"
BASE COUNT          723 a         1578 c         1515 g          691 t
ORIGIN      
        1 cgcctacgca aactggaagg acggcgagcg cgcttccgcg atcgcggcgg ccgaccgctt
       61 catcaagctc tatccgaacc accccaacgt cgactacgcc tggtacctga aaggcctgat
      121 caacttcaac gagaacctga gcgtcctgtg gtggctcacc acgccggacc tgtcggatcg
      181 cgatccgcgg ggctcgcgcg atgccttcga tgccttcaag gaagtggtac agcgttttcc
      241 ggaatcgaag tacgccgcgg attccgcggc gcgcatgcgc tacctggtca acgccctcgc
      301 cagctacgag gtgcacgtcg cgcgctatta catgaggcgc ggcgcctatc tcgccgccgc
      361 caaccgggcg cagtacgcga tcaagagcta cccgcaggcg ccggcgctgg aagaggcggt
      421 gttcgtgctg gtgaaagcct acgacgcgct cgacatgggc gagctgcgcg acgctgcggc
      481 gcgcgtcatg cgcgtcaatt ttcccgagag ccgctacctc aaggtcggcg acgtcaggcg
      541 cgacgcaccg tggtggcgga tctgggatcc ggactggtga agcccggggc gaggcggatt
      601 ctcgtcaccg gcgcggccgg gatgatcggc cgcaagctca cgcagcggct cgtcagggaa
      661 ggtgccggcg aactcatcct gcatgacgtc gtgccgttcg gcgagaacac cgtggtatcg
      721 gacctggcaa cgcccggaga agcggaaaag ctgctcgagc ggcgccccga cctcatcttt
      781 catctcgccg ccatcgtctc cggcgaagcg gaagccgatt tcgagaaggg ctaccgcgtc
      841 aatctcgacg gcacgcggcg ccttttcgaa gccattcgaa aaatacagaa tggctataag
      901 ccgcgcctgg tcttcacttc gtccatcgcg gtgtttggcg cgccgtttcc ggaggcgatc
      961 ggcgacgagt tcctcagcgc gccgctcacc agctacggca cgcagaaggc gatcagcgag
     1021 ctgctgcttg ccgactgcag ccgccgcgga ttcttcgacg gcatcggcat acggctgccg
     1081 accatctgcg tgcgcccggg caagcccaac ctggccgcgt ccggcttttt ctccggcatc
     1141 atccgcgagc cgctgaacgg cgaggaagcg atgctgccgg tgccggaaac cgtgcggcac
     1201 tggttcgctt cgccgcgcgc cgccataggg tttttgattc atgcatcaaa aatcaatact
     1261 gaacacctcg gcgcgcgccg caacctcagc atgccgggcg tcgccgcgac ggtcgcggag
     1321 cagatcgagg ccctgcggcg cgtcgccggc gagcgggtgg cgcgcctgat ccgccgccgg
     1381 cccgacccgg cgatcctgcg catcgtcgag ggctggccgc ggaatttcga tgcgcagcgc
     1441 gcgctcgcgc tcggctttcg cgccgacacg agcttcgagg agatcatccg gattcacatt
     1501 caggatgaga tgcagacgga tgagctgcca ggaacccctc gacctcggat aggttccgcg
     1561 tgagcttcat cgccggcagg ctgcgcagaa tctgcctgcc ataggcgcgc gacgcgagcc
     1621 gcggatcgca cagcatcagc acgccgcggt cggtctcgtc gcggatcagg cgccccgcgc
     1681 cctgcttgag ctgcagcacg gcctgcggca gctgcagctc ggtgaatgga ttgccgccct
     1741 gcacgcggat ggcgtcgatg cgcgccgcga gcaccggatc gtcgggcggc gcgaacggca
     1801 gcttgtcgat caccaccagc gacagcgcct cgccgcgcac gtcgacccct tcccagaacg
     1861 actggctgcc gagcagcacc gcgttgccga gcgcgcggaa gcgctcgagc agctcgctgc
     1921 gcgacccggt gccctgcacc agcagcggaa agcgcagccg cccgcgcagc agctcgtgcg
     1981 cccggcgcag cgcgcgcagc gtggtgaaaa gcaggaacgc gcgccccgcg ctcgcctcga
     2041 gcaccggagc cgcggcctcg atcacggcgt cggtgaacgc ggcgtcgttc gggtcggccg
     2101 gcagcccctg cggcaggtac agcagcgcct ggcggccgaa atcgaacggg ctcgcccagc
     2161 ggcgcgcagc ggcctgcggg atgccgagct cgcgcgtgaa atgcgcgaag tcgtcgccga
     2221 ccgcgagtgt cgccgaggtg aagatccagg ctcggggatg atcgctcatc tggcggcaga
     2281 acagctcggc cggcgaaagc ggcgtgaggt gcaggcgggc cgcgccgccg aaaacctcga
     2341 cccagcgcac ctcgtcgcgc gcttccgact cgcgcaggcg cacgagcagg gcgagcgccg
     2401 cgcccgcgcg ccgcgcgcac gaggcgagcc cttccgagcg ctcggactgc gtctcgagcg
     2461 gcgcttgaag tttctttaag gaagcattca atccttcaat cgcgtccgga aattcagcga
     2521 ggcgcatcgc ctggttccag cccaggcgcg cgccggcctc gccgagggag aggcgcagat
     2581 cgcgcgccgc gcgctcgagg ctcgccgcca gccgatcgag ctcgggcgat gcgccgcccg
     2641 cggcgcgcag ctccaggcgc gcgtcgcgcg agagctcgac cagctgggcg ctcgagacgg
     2701 tgtcgccgaa gaacaggcgc gccgtctcgg gcaactggtg cgcctcgtcg aacaccagcg
     2761 tgttgcacgc cggcagcagc tcggcgatgc cctcgtcgcg cagcaccacg tcggcgaaga
     2821 acaggtgatg gttgatgacc accacgtcgg cggcaagcgc gttcctgcgc gcctgcatca
     2881 cgaagcactc ggaatagtcg gggcaagcct ggccgaggca gttgtcgcgc gtcgatgtgg
     2941 cgtgcgtcca gaccgcggcg tcctcgggca cgtcggcgag atcggcgcga tcgccggtgg
     3001 tgctcactgc cgcgaagcgc tcgatgcgct gcagctgcga ggcttcttcg cgggtcgcga
     3061 agcgcgcttc ggcagtcgcg cggcgcaggc ggtagcggca cacgtagttg gcgcggccct
     3121 tgagcagcgc cgcgctcgcg cccgagccga gcgcctcgcg cacggcgggg aggtcgcggt
     3181 cgaagagctg gtcctgcagc gttttggttc cggtcgacac cacgaccttg ccgcccgcga
     3241 gcagcgccgg caccaggtag gcgaaggtct tgcctgtccc ggtccccgcc tcggccacca
     3301 gcacacccag gcgctcgatc gcctcgtgga tcgcctcggc catctgcacc tgctgcggac
     3361 gcagcttgaa acccggcacc gccgcggcga gcgggccgtc aggcgcaaag atccgctgca
     3421 gcgctcgctt agacaagaag cgctgcgcgc attttcagtt gcgcacctct tcgaccttca
     3481 cccagatgcg gcgcgactgc aaggcgcgcg cgccgcttgc cgaagcgatg ccgcggtccg
     3541 cgcgcgtcgc gctcgagccg acggcgccga gctcgaacca ttctccaagg cggcccgtca
     3601 cgctgctcga ggcgcgctcg aacgactgcg gcgtctcgcg ctgcggcgcg agctcgagat
     3661 atacgttctc gccggatatg cgcggcacca cctcgaagcc ggttgccagg tcctgaaccg
     3721 cgacgacttc gcgggctacc acggggcgcg attgcccggt gaagatcatg gcgcgaccgc
     3781 cctcgagcac ctgcacgcgc tgatcgacgc gctcgtccgc ccgcgagctc gactgccgcg
     3841 cctgcacctc caggcgcgag ccacgattgc tgatgcgacc gctcgcttcg acgccctcgc
     3901 tcgatgcttc gagcgcgtcg tcgaagcgca ccgagatctg caatcgtcgc aatggccggt
     3961 cgatggcgtc gagggcgcgc cggatgtcag cgagattggc ggggctagcg cgcactatca
     4021 actggtttcc ctggccggtg agcgtgccgc caggctcgac gagcggccgt aacgtcggca
     4081 atacctgctc gacggtacgg tgcctgagcg ggatgatttc cagcgcgtgc tgcgctgcgc
     4141 tcaagctcgc ggcacacgcg gccgcgactg cgagcgtcga tcggaggagt ttcattcgat
     4201 tcaccttcac atcatgaacg cgcgagctgc gggcgcgtct acgccgtttg cacaagccat
     4261 cgcctgcagg ctatcgtcag acacaagctt ctgtttctgt gctgctttct cgcggcttca
     4321 gtcctcgagc ccgtataccg cccgcacctc aaaagcgaat tccttgccgc gcaacgcaag
     4381 cggcaccggc acggcttgcg tcgcctgcct gaacatctcg agcgcctgct ggtcgaggat
     4441 ctggtagccg gagctcgctt tcaggtttag cgccgctgct ccgttcgcag caacggcgac
     4501 gccgatc
//
LOCUS       DASPXW010000038         2843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_12252, whole genome shotgun sequence.
ACCESSION   DASPXW010000038 DASPXW010000000
VERSION     DASPXW010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12252"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="VEL04_00835"
     CDS             complement(<1..474)
                     /locus_tag="VEL04_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYT46939.1"
                     /translation="MSPLLKASGITKRFGSRTLFNRASLELETGNGYVLTGPNGSGKT
                     TLLRVLAGLDTAEQGSISYRGASASAAGRYPEPWRREIVYVHQQPYLFRTSLQGNVEY
                     GLARRGVANERRRAVAREALAWAGLAAREHVPPHRLSGGEKQRAALARAKVLDPVL"
     gene            complement(471..1166)
                     /locus_tag="VEL04_00840"
     CDS             complement(471..1166)
                     /locus_tag="VEL04_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYT46940.1"
                     /translation="MDLLEPTRQAFALLFSGDRELWTIIWLSVWVSVAALALLAPLCV
                     PLGFVIAHVAFPGRRALVVLMQGFLSFPTVVVGLVLYLLLSRRGPLGAFELLFTPGAI
                     LMGYMVIALPILMVFTVSAVQGADARIYETARCLGAHRVRAMLTTLWEVRFAVTAGFV
                     NAFGRVVSEVGCALLVGGNIAGLTRNIPTAIALETSKGEFAFGIALGFVLMVIAVGVN
                     IALAWLQGEGGLE"
     gene            complement(1173..1970)
                     /locus_tag="VEL04_00845"
     CDS             complement(1173..1970)
                     /locus_tag="VEL04_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HYT46941.1"
                     /translation="MMIFRSLLLGIALLATAAHAQTVRMATTTSTANTGLLDDLLPKF
                     RERTGIAVQYVAVGTGAALKIGEQGDADLVLVHDRESEERFLAKGYGVNRRDLMYNDF
                     IIVGSPRDPAGIRSVRDAAEALKRIRAAGAAFVSRGDQSGTHLRELSLWRAAGVQPKW
                     EGYYLSIGQGMGTALIMTYEKRGYTLSDRGTFLAYRGKTDLEIMVEGDRRLHNPYGLI
                     AVNPARHPKINYSGAMQLIGWMTGPEGQKAIADFKIGGAQLFFPAAK"
     gene            2109..2513
                     /locus_tag="VEL04_00850"
     CDS             2109..2513
                     /locus_tag="VEL04_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HYT46942.1"
                     /translation="MDAGITPAALRQAIGSEVSPLLIDVRRRQSFLESDCIEFSHVGE
                     RCSFDAFLAKFHLKDPALDQLALIVRGADTNRLDLAPQAAGLAAISLGLSRIDENDHA
                     MLEHGVIVYDALYRWCKEGKQETHTWNPEAYK"
     gene            2510..>2843
                     /locus_tag="VEL04_00855"
     CDS             2510..>2843
                     /locus_tag="VEL04_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HYT46943.1"
                     /translation="MKPAILPLPFDAARLRGLSERLLQSHHQNNYAGAVKRLHAIRAE
                     LAALDWGSASGFRINGLKREELIAANSMILHEIYFDGLGGGGAPRGTLAETIARDFGG
                     LDRWRTQFS"
BASE COUNT          497 a          970 c          929 g          447 t
ORIGIN      
        1 cagcaccggg tcgagcacct tggcgcgggc gagcgccgcg cgctgcttct cgccgcccga
       61 gaggcggtgg ggcggcacgt gctcgcgcgc cgcaagcccc gcccaggcga gcgcctcgcg
      121 cgcgacggcg cgccgccgct cgttcgccac gccgcgccgc gccagcccgt actcgacatt
      181 gccctgcagc gaggtgcgaa acagataggg ctgctggtgc acgtaaacga tttcgcgccg
      241 ccatggctcc gggtagcggc cggccgcgct ggccgatgcg ccgcggtaac tgatggatcc
      301 ctgctccgcg gtatcgaggc ccgccaggac gcgcagcagc gtcgtcttgc cgctgccgtt
      361 cgggccggtg agcacgtagc cgttgccggt ctcgagctcg agcgacgccc gattgaacaa
      421 cgtacggctg ccgaagcgct tggtgatgcc cgaggctttg aggagcgggc tcattccagg
      481 ccgccctcgc cctgcagcca cgcgagcgcg atgttgacgc ccaccgcaat caccatcagc
      541 acgaagccga gcgcgatgcc gaaggcaaac tcgcccttcg aggtctcgag cgctattgca
      601 gttggaatgt tacgtgtgag cccggcgatg ttgccgccga cgagcagcgc gcagcccacc
      661 tccgagacca cgcgaccgaa ggcattcacg aaaccggccg tcacggcgaa gcgcacttcc
      721 cacagtgtgg tcagcatcgc gcgcacgcgg tgcgcgccga ggcagcgcgc ggtctcgtaa
      781 atgcgcgcat ccgcgccttg caccgccgac accgtgaaca ccatcaggat cggcagcgcg
      841 atcaccatgt agcccatcag gatcgccccc ggcgtgaaga gcagctcgaa agccccgagc
      901 ggtccgcggc gagaaagcag caggtataga acgaggccga ccaccacggt aggaaacgaa
      961 aggaacccct gcatcaggac gacgagggcg cgccgccccg gaaacgccac gtgcgcgatg
     1021 acgaagccga gcggcacgca cagcggcgcg agcagcgcga gcgcggcgac cgacacccac
     1081 accgagagcc agatgatggt ccacagctcg cggtcgccgc tgaaaagcag cgcaaaggcc
     1141 tggcgggtgg gctcgagcag atccatcggc cgctatttcg ccgccgggaa aaacagctgc
     1201 gcgccgccga tcttgaagtc ggcaatcgcc ttctggccct cgggcccggt catccagccg
     1261 atcagctgca tcgctccgga gtagttgatc ttcgggtggc gcgcaggatt gacggcgatc
     1321 aggccgtagg ggttgtgcag ccgcctgtcg ccttcgacca tgatctcgag gtcggtcttg
     1381 ccgcggtagg cgaggaaggt gccgcggtcg gaaagcgtat agccgcgctt ctcgtaggtc
     1441 atgatgagcg cggtgcccat gccctggccg atcgacaggt agtagccttc ccatttcggc
     1501 tgcacccctg ccgcgcgcca cagcgacagc tcgcgcaggt gcgtgcccga ctggtcgccg
     1561 cgcgagacga aagcggcgcc ggccgcgcgg atgcgcttca gcgcctcggc cgcgtcgcgc
     1621 acactgcgga ttcctgccgg atcccgaggc gagccgacga tgatgaagtc gttgtacatc
     1681 aggtcgcggc ggttcacccc atagcccttg gcgagaaagc gttcctccga ctcgcggtcg
     1741 tggaccagca ccaggtcggc gtcgccctgc tcgccgatct tcagcgccgc gccggtgccc
     1801 accgcgacgt actgcaccgc gatgccggtg cgctcgcgga atttcggcag caggtcatcg
     1861 agcaggccgg tgttggcggt gctggtcgtg gtcgccatcc gcaccgtttg cgcgtgggcg
     1921 gcggttgcga gcaatgcaat acccagtaaa aggctacgaa agatcatcat ggcaagcggc
     1981 gattgtactt gccgccttga caaccccagc cggccgagag cactctatgc ggcctcgggg
     2041 atcgcaggct ccccgcatgt caaagacgct gtgcgtccaa gctctgcaag actctgaagg
     2101 agagagccat ggacgctggc atcacccccg ccgctctgcg gcaagcaatc ggttccgaag
     2161 tttccccgct gctcatcgac gtacgccgca ggcagtcgtt tctcgaatcg gattgcatcg
     2221 agttctcgca cgtcggcgag cgctgcagct tcgacgcttt tctcgcaaaa tttcatctca
     2281 aggatcccgc gctcgaccag ctcgcgctca tcgtgcgcgg cgcggacacc aaccggctcg
     2341 atctcgcgcc ccaggcggcg ggactggccg cgatctccct tggactgtct cgtatcgatg
     2401 aaaatgatca tgcgatgctc gagcacggcg tgatcgtcta cgacgcgctc taccgctggt
     2461 gcaaggaagg caagcaggaa acccacacct ggaatccgga ggcatacaaa tgaaaccggc
     2521 gatccttccc ctgcctttcg atgcggcgcg gctgcgcggt ctctcggagc gtctgctgca
     2581 atctcatcat cagaacaact atgccggggc ggtcaagcgg ctgcacgcga tccgcgccga
     2641 gctcgcggcg ctcgactggg ggtcggcgtc cggcttccgc atcaatggcc tgaagcgcga
     2701 ggagctgatc gccgccaact cgatgatcct gcatgagatc tatttcgacg ggttgggcgg
     2761 cggtggtgcg ccgcgcggca cgctcgccga gaccatcgcg cgcgatttcg gcggcctcga
     2821 tcgctggcgc acccagttct cgg
//
LOCUS       DASPXW010000039         3592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1274500, whole genome shotgun sequence.
ACCESSION   DASPXW010000039 DASPXW010000000
VERSION     DASPXW010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274500"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="VEL04_00860"
     CDS             <1..674
                     /locus_tag="VEL04_00860"
                     /inference="COORDINATES: protein motif:HMM:NF015818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HYT46944.1"
                     /translation="AEADGLAALRNAGLRSPEPLAHGVAGEQAFLLMEFLQLQSNGDF
                     AALGKMLAAAHRHTGPRFGWRRDNYIGATPQANGWCDDWAEFWRERRLLPQLELARRN
                     AFDLKADSLDFALEKHRPQASLLHGDLWSGNAAFTAVGEPLVFDPAVYYGDREADLAM
                     TELFGGFPGDFYRAYREAWPLDPGYEKRKHLYNLYHLLNHLNLFGGGYLGQVKTTLGL
                     LLRAS"
     gene            complement(648..971)
                     /gene="grxD"
                     /locus_tag="VEL04_00865"
     CDS             complement(648..971)
                     /gene="grxD"
                     /locus_tag="VEL04_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HYT46945.1"
                     /translation="MDTQNRIKQQVTGNHVVLYMKGTPQMPQCGFSALACQVLNACGV
                     QDFFTVNVLADAEIREGIKQYANWPTIPQLYVNGEFVGGSDIMREMYQSGELQKILRG
                     SKQQA"
     gene            complement(981..1769)
                     /gene="prmC"
                     /locus_tag="VEL04_00870"
     CDS             complement(981..1769)
                     /gene="prmC"
                     /locus_tag="VEL04_00870"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HYT46946.1"
                     /translation="MTVQEALSSSGIDPREARLLLAQACGFSEAAVVAFPERKLPQDT
                     ENCFADFVSRRRSGEPIAYIIGHKEFYGLELAVNPAVLIPRPETELLVELALRHAFSS
                     VADLGTGSGAVALALKKHRPEAQVVAVEASAAALATARRNSTRHGLEIRLVHGLWFQP
                     LAGERFDLIVANPPYVAVGDPHLSELRFEPQGALIAGADGLDAMREIAREAANHLLPG
                     GRLLLEHGQGQEEAVRLLLDAAGLEEIATWRDLAGIARITGGKR"
     gene            complement(1766..2851)
                     /gene="prfA"
                     /locus_tag="VEL04_00875"
     CDS             complement(1766..2851)
                     /gene="prfA"
                     /locus_tag="VEL04_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HYT46947.1"
                     /translation="MKPSLRAKLDGLAGRLEDLNRLLAAEDAAKNLEQFRKLSREHSE
                     LSGIVSLYGRWRQSERDADAAKEMTSDAAMKTFADDELKSARSSMEKLEAELHKALLP
                     KDPNDERNIFLEIRAGAGGDESALFAGELFRMYARYAERQGWQVEIISESPSELGGYK
                     EVIARIVGQRPYSKLKFESGGHRVQRVPETEAQGRIHTSACTVAVLPEADAVDEVVLN
                     PAELRIDTFRASGAGGQHVNKTESAIRVTHLPTGIVVECQDSRSQHKNREKALAVLAA
                     RIRDKQLREQQAKTASTRKSLIGSGDRSERIRTYNYPQGRVTDHRINLTLYKIDRILD
                     GELDELIGALEAEHRTEQLSQLAEEAA"
     gene            complement(2871..>3592)
                     /gene="hemA"
                     /locus_tag="VEL04_00880"
     CDS             complement(2871..>3592)
                     /gene="hemA"
                     /locus_tag="VEL04_00880"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HYT46948.1"
                     /translation="LKDQSVLLIGAGEMIELCATHFAAQGPARITVANRTLERAERLA
                     HRFNGRAIELRSLAEQLHEYDIVVSCTASSLPILGKGLLERALRTRRRRPIFMVDLAV
                     PRDIEQEVAELDDVFLYTVDDLGEIVSKNLDTRRSAVEQAEAIIDTQVGQFMHWMQVR
                     ESVPLIRMLREQAEAARRHELERALKLLARGEDPAKVLETLSQAITNKLMHAPTRALN
                     ESAGEEHRALAEALSRLLRTK"
BASE COUNT          547 a         1196 c         1192 g          657 t
ORIGIN      
        1 ccgccgaggc cgacggcctg gccgcgctgc gcaatgccgg cttgcgctcg cccgagccgc
       61 tggcccacgg cgtggccggc gagcaggcat tcctcctaat ggagtttctg cagctgcaat
      121 cgaatggcga ctttgcggcc ctggggaaaa tgctcgccgc ggcgcatcgc cacaccgggc
      181 cgcgcttcgg ctggcggcgc gacaactaca tcggcgccac cccgcaggcg aacggctggt
      241 gcgacgactg ggccgaattc tggcgcgagc ggcggcttct gccgcagctc gaactcgcaa
      301 ggcgcaacgc gttcgacctc aaagcggatt ccctggattt cgctctggaa aagcaccgcc
      361 cgcaggcctc actgctgcat ggcgatctat ggagcggaaa cgccgctttc actgccgttg
      421 gcgagccgct ggtgttcgac ccggcggtgt attacggcga ccgcgaggcc gatctcgcga
      481 tgaccgagct gtttggtgga tttcccgggg atttctaccg cgcctaccgt gaagcctggc
      541 cgctcgatcc cgggtacgag aagcgaaagc acctctacaa cctgtatcac ctgctcaacc
      601 acctgaacct tttcggcggt ggctacctcg ggcaggtgaa gacgacttta ggcctgctgc
      661 ttcgagcctc gtaggatctt ctgcagctcg ccggactggt acatctcgcg catgatgtcc
      721 gagccgccga cgaattcgcc gttcacatag agctgcggga tcgtcggcca gttggcgtac
      781 tgcttgatgc cttcgcggat ctcggcatcg gcgagcacat taacggtaaa gaagtcctgc
      841 acgccgcagg cattcagcac ctggcaggcg agcgccgaaa agccgcattg cggcatctgc
      901 ggtgtgccct tcatatagag cacgacgtgg ttgccggtga cctgctgctt gatgcgattt
      961 tgggtgtcca tggtgcgatt ttatctcttt ccgccggtga tccgtgcgat tcctgccaga
     1021 tcgcgccagg tcgcgatttc ttcaagccct gctgcatcga gcagcaagcg cacggcctcc
     1081 tcctgcccct ggccgtgctc gagcagcagg cgccctcccg gcaggaggtg gttcgcggcc
     1141 tcgcgcgcaa tctccctcat ggcatcgagg ccgtcggcgc ctgcgatcag cgccccctgc
     1201 ggctcgaagc gcagctcgga aagatgcgga tcgcccacgg cgacgtaagg cgggttcgcg
     1261 acgatcagat cgaagcgctc gccggcgagc ggctgaaacc agagcccgtg aaccagccga
     1321 atttccagcc cgtgtctggt ggaatttcgc cgtgccgtgg cgagcgcggc agcgcttgcc
     1381 tccacggcca cgacctgcgc ctcgggccga tgctttttga gcgcgagcgc gactgcgccc
     1441 gatcccgtgc ctaaatcggc aacgctcgag aacgcgtgtc gcagcgctag ctcgacgagg
     1501 agctcggtct cggggcgcgg gatgaggacg gccggattga cggccagctc gaggccgtag
     1561 aactccttgt ggccgatgat gtaggcgatc ggttcaccgc ttctgcgccg ggaaacaaaa
     1621 tcggcaaagc agttttccgt gtcttgcgga agctttcgct ctggaaaggc gaccacggcc
     1681 gcttcggaaa aaccgcaggc ctgggcaagc agcagacgcg cctcgcgcgg gtctatgccg
     1741 ctcgagctca acgcttcctg aacggtcaag ccgcttcttc ggcgagctgc gagagctgct
     1801 cggtccggtg ctcggcctcc agcgcgccga tcagctcgtc gagctccccg tccaggatgc
     1861 ggtcgatctt gtagagcgtg aggttgatgc ggtggtcggt gacgcggccc tgaggatagt
     1921 tgtaggtgcg gatgcgctcc gagcgatccc ccgagccgat caggctcttg cgcgtggagg
     1981 cggtcttcgc ctgctgctcg cgcagctgct tgtcgcggat gcgcgcggca agcaccgcga
     2041 gcgccttctc gcggttcttg tgctgcgagc gcgagtcctg gcactcgacc acgatcccgg
     2101 taggcaggtg cgtgacgcgg atcgcggact cggtcttgtt gacgtgctgc ccgcccgcgc
     2161 ccgaagcgcg gaacgtgtca atgcgcagtt ccgccggatt cagcaccacc tcgtcgaccg
     2221 cgtcggcctc gggcagcacc gccaccgtgc acgccgaggt atggatgcgc ccctgcgcct
     2281 cggtctcggg cacgcgctgc acgcgatggc cgcccgactc gaacttgagc ttcgaatagg
     2341 gccgctgccc gacgatgcgc gcgatgacct ccttgtagcc gccgagctcc gaggggctct
     2401 ccgagatgat ctcgacctgc cagccctggc gctcggcata gcgagcgtac atcctgaaca
     2461 gctcgcctgc gaacagcgcc gattcgtcgc ctccggcgcc ggcgcggatc tcgaggaaga
     2521 tgttgcgctc gtcgttcggg tccttcggca gcagcgcctt gtgcagctcg gcctcaagct
     2581 tttccatgct cgagcgcgcg cttttcagct cgtcgtcggc gaatgtcttc attgcggcgt
     2641 cggaggtcat ttcctttgcc gcgtcggcat cgcgctccga ctgcctccag cgcccgtaaa
     2701 gcgagacgat cccgctcagc tcggagtgct cgcgcgacag cttgcggaac tgctcgaggt
     2761 tcttggccgc gtcctcggcg gcgagcagcc ggttgaggtc ctcgaggcgc ccggcgaggc
     2821 cgtcgagctt cgcgcgcagc gaaggtttca cctggtgcgc aggagcggat tcacttcgta
     2881 cgaagcaggc gcgacagcgc ttcggcgagc gcgcgatgct cctcgcccgc gctctcgttg
     2941 agcgcccgcg tcggcgcgtg catcagcttg ttggtgatcg cctgcgagag cgtttccagg
     3001 accttcgccg gatcctcgcc gcgcgcgagc agcttgagcg cgcgctcgag ctcgtggcgg
     3061 cgcgccgcct cggcctgctc gcgcagcatg cggatgaggg gcacgctttc ccgcacctgc
     3121 atccagtgca tgaactggcc gacctgggtg tcgatgatcg cctcggcctg ctcgacggcg
     3181 gaacggcgcg tgtcgaggtt cttcgagacg atctcgccga ggtcgtcgac cgtgtacagg
     3241 aacacgtcat cgagctcggc gacctcctgc tcgatgtcgc gcggcacggc aagatcgacc
     3301 atgaagatcg gccggcgccg ccgcgtgcgc agcgcgcgct cgagcagccc cttgccgagg
     3361 atcggcagcg acgaggccgt gcaggaaacg acgatgtcgt attcgtgcag ctgctcggcg
     3421 agcgagcgca gctctatggc gcggccgttg aagcggtgcg cgaggcgctc ggcgcgctca
     3481 agggtgcggt tggcgacggt gatgcgcgcc ggcccctgcg cggcaaagtg cgtcgcacac
     3541 agctcgatca tctctcctgc gccgatgagc agcacgctct ggtccttgag gc
//
LOCUS       DASPXW010000040         5685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_895306, whole genome shotgun sequence.
ACCESSION   DASPXW010000040 DASPXW010000000
VERSION     DASPXW010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5685
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_895306"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VEL04_00885"
     CDS             complement(<1..443)
                     /locus_tag="VEL04_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT46949.1"
                     /translation="MLAARAAAGAPDTVRVALSGGDPAAVSRQLHYLGYLSRAAAALI
                     GGYRSGLAELERLARDADARRARLRAVEQASRADRERILKERRARRGVLERIAGDIRA
                     SRKQIRVLRADEARLGRLIEGIGRVLARVEATPEPGSAKEAFSTLR"
     gene            complement(749..1489)
                     /gene="gpmA"
                     /locus_tag="VEL04_00890"
     CDS             complement(749..1489)
                     /gene="gpmA"
                     /locus_tag="VEL04_00890"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015877095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HYT46950.1"
                     /translation="MPKLVLLRHGESDWNRENRFTGWTDVDLSQRGVEEARAAGRSLA
                     AGGYRFDLAFTSVLKRAIRTLWLALDELDQMWLPVEKSWRLNERHYGALQGLNKAEMA
                     AKFGEQQVLVWRRSYDTRPPELNPNDSRHEAKDPRYAGVAVPATECLKDTVARVIPYW
                     ASAIAPAVSAGRRVLIAAHGNSLRALVKHLDNISDAGIVKENIPTGIPLVYELDDRLR
                     PLRHHYLGDPDEVAKRIAAVSAQGKAKV"
     gene            1517..1918
                     /locus_tag="VEL04_00895"
     CDS             1517..1918
                     /locus_tag="VEL04_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003278638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HYT46951.1"
                     /translation="MKFIQDNLLLIAVAFASGAMLIWPLVRRTTGGPWVNPARATELI
                     NREDALVLDVREPGEYGAGHILGAKNVPASRLEAAELGKRMERPLIVYCDGSERSAKA
                     AAALKRMGFTRVANLSGGLAAWQQAGLPVEK"
     gene            1935..2195
                     /gene="grxC"
                     /locus_tag="VEL04_00900"
     CDS             1935..2195
                     /gene="grxC"
                     /locus_tag="VEL04_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004200200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HYT46952.1"
                     /translation="MARVLMYCTSACPFCESAERLLVGKGARVEKIRVDLEPARRAEM
                     MQRSGRRSVPQIWIGARHVGGCDELYALEREGKLAPLLEASP"
     gene            2192..2683
                     /gene="secB"
                     /locus_tag="VEL04_00905"
     CDS             2192..2683
                     /gene="secB"
                     /locus_tag="VEL04_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018911771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HYT46953.1"
                     /translation="MTEAQPPNSPAQPQQPATFQIEKLYVKDLSLEIPHAPQVFAEKA
                     QPQIEVHINTGESQFSEGYYEVTVSATVTARAGERTLFLAEAVQAGIFALRNVPAAEL
                     GALLGIACPTILFPYLRETISDLVIRGGFPPLLLSPISFEQLYLQRLQQQGGEGPRIE
                     VAQ"
     gene            2687..3664
                     /locus_tag="VEL04_00910"
     CDS             2687..3664
                     /locus_tag="VEL04_00910"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HYT46954.1"
                     /translation="MRIAVLGAGAWGTSIASVLGARLEVTLWARDAAQARAIEQTRRN
                     ERYLPGIPVPASVVVSSDLSKTVAGARLVLAATPVAGLRELLGKIRSVTSLVWLCKGF
                     EEGSGALPHQIAAEVLGEAARCGALSGPSFAEEVARGLPCALTLASRDAAFARETAAL
                     LHGGRLRVYYSADLVGVEIGGAVKNVMAIAAGISDGLGLGHNARAALITRGLAEIARL
                     GAALGGAPQTFMGLAGAGDLILTATGDLSRNRRVGLELAQGRTLNQVLKNLGHVAEGV
                     RSAKEVARLAAARGVDMPVTDAVNAVLAGKLTPAAAVERLLSRDPKKER"
     gene            complement(3661..4125)
                     /locus_tag="VEL04_00915"
     CDS             complement(3661..4125)
                     /locus_tag="VEL04_00915"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HYT46955.1"
                     /translation="MFDIVLVHPEIPPNTGNVIRLAANTGARLHLVRPLGFSLDDKQL
                     KRAGLDYHALASLTVHADWNACRDALGGRRMFALTTRAARNYAEVKFADDDVFVFGAE
                     TAGLPHELLDQFETEMRLRLPLLPGNRSLNLSNAVAVVVFEAWRQLGFRGAG"
     gene            complement(4118..4792)
                     /locus_tag="VEL04_00920"
     CDS             complement(4118..4792)
                     /locus_tag="VEL04_00920"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HYT46956.1"
                     /translation="MHAKLAAALFGGTCFLCRGAARAPLCAACDADLPRLAGPLCPRC
                     ALGSPSGELCGRCLAEAPHYDATVAALAYRFPADALVHALKFRGELALVSLFGNLLLQ
                     KIPADQAVDCVIPVPLSPHRLRERGYNQALEIARHVKPGKIDLAACVRQDNAPPQTGL
                     PLEERQRNVRGAFRCTRSLAGARVAVLDDVMTTGATLDEMARTLKGSGAAHVVNWVVC
                     RTPADV"
     gene            4807..>5685
                     /locus_tag="VEL04_00925"
     CDS             4807..>5685
                     /locus_tag="VEL04_00925"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HYT46957.1"
                     /translation="MWTVAQLAADLASGATTSRELVEQAFTRIVDPAGEGARAFVKMY
                     ADSARAEADFSDRLRHAGVLRSPVDGLPVSVKDLFDVAGDVTRAGSRILETASPAKAD
                     AAAVARLRAAGTVLIGRTNMVEFAFGGVGLNPHFGTPKNPYDRKTGRVPGGSSSGAAV
                     AQADGMCVMALGSDTRGSIRQPAALCGVAGFKPTARRVPREGAFPLSFTLDSIGPLAN
                     SAACCATYDAILAGEPPLPLPEMPAKGLRLLLPRSSALEDLDPQVGKAFDVALARLSR
                     AGMLVIEIPVPAFDRQN"
BASE COUNT          837 a         1954 c         2024 g          870 t
ORIGIN      
        1 cgcagcgtgg aaaaggcttc ctttgcggag cccggctcgg gcgtggcctc gacgcgcgcg
       61 aggacgcggc cgattccctc gatcaggcgt cccagccggg cctcgtcggc acgcagcacc
      121 ctgatttgct tgcgactggc gcggatgtcg ccggcgatgc gctcgagcac gccgcgccgc
      181 gcgcgacgct ccttgaggat tctttcacgg tcggcgcggc tcgcctgctc caccgcgcgc
      241 aggcgcgcgc gcctggcgtc ggcgtcgcgc gcgagccgct cgagctcggc gagcccgctg
      301 cggtagccgc cgatcagggc cgcggcggcg cgcgaaaggt agccgaggta atgcagctgc
      361 cgcgagaccg cagccggatc cccgccggag agcgccacgc gcacggtgtc gggagcgcct
      421 gcggcggcgc gcgcggcgag catgcgctcg agcgcggcgc gccgctcggc gagctggcgc
      481 tcggtgccgc ggcggcgctc ggcgatgcga gcggcgtcgg cgcgcaggcc gcgcgcctcg
      541 gactgcagcg cgaccagggt gcggttcgca tccgagatgg cgcgctcgga atcgcgcagc
      601 gcgtcgcgcg cttcgcgccg cgactcttcc ttggtttcca gttcccggct taaggacgcg
      661 atgcgggagc gcagtacctg cagatcttca tcgcgcgacg ctgcctgtgc ggcgcctgcc
      721 gcaatggcga ggaggaatag cgcgtgcctc agaccttcgc cttgccttgc gccgagaccg
      781 ccgcgatgcg cttcgccact tcgtcgggat cgcccagata atggtgccgc agcggcctca
      841 gccggtcgtc gagctcgtac accagcggga tgccggtggg aatgttttcc ttcacgatgc
      901 ctgcgtcgga aatgttgtcc agatgcttga ccagcgcgcg cagcgagttg ccgtgcgccg
      961 cgatcagcac ccggcgcccg gcgctcaccg ccggcgcgat cgcgctcgcc cagtaaggaa
     1021 tgacgcgcgc cacggtgtcc ttcaggcatt cggttgccgg gaccgcgacg ccggcgtagc
     1081 gtggatcctt cgcttcatgc cttgagtcat tggggttgag ttcagggggg cgcgtgtcgt
     1141 agctgcgccg ccagacgagc acctgctgct cgccgaattt cgccgccatc tcggccttgt
     1201 tcaggccttg cagagcgccg tagtgccgct cgttcagccg ccagctcttc tcgaccggca
     1261 gccacatctg gtcgagctcg tcgagcgcga gccacagcgt gcggatggcg cgcttcagca
     1321 ccgaggtgaa ggcgagatcg aagcgatagc cgcccgcggc gaggctgcgg ccggcagcgc
     1381 gcgcctcctc gacgcctctc tgcgaaagat cgacgtcggt ccagcccgtg aagcggttct
     1441 cgcggttcca gtccgattcg ccgtggcgca ggaggacgag cttcggcatc gcgctgaatt
     1501 ataatgtccg cccctcgtga aattcatcca ggacaacctt ctgctgatcg ccgtcgcgtt
     1561 cgcctccggc gcgatgctga tctggccgct cgtgcgccgc accaccggcg gaccctgggt
     1621 gaatcccgcc cgcgccaccg agctcatcaa ccgcgaggac gcgctggtgc tcgacgtgcg
     1681 cgagccgggc gagtacggcg cgggccacat cctcggagcg aagaacgtgc ccgcttcgcg
     1741 cctcgaggcg gccgagctcg gcaagcgcat ggagcggccg ctgatcgtgt actgcgacgg
     1801 cagcgaacgc tcggcgaagg cggccgcggc gctcaagcgc atggggttca cgcgcgtggc
     1861 caatctctcg ggcggcctcg cggcctggca gcaggcggga ttgccggtgg agaaataacc
     1921 ggagaaatga gggcgtggct cgggtcctca tgtactgcac ctcggcgtgc ccgttctgcg
     1981 agtcggccga gcggctgctg gtcgggaaag gcgcgcgggt cgagaaaatc cgcgtcgacc
     2041 tcgagcccgc gcggcgcgcg gagatgatgc aaaggagcgg ccggcgcagc gtgccgcaga
     2101 tctggatcgg cgctcgccat gtcggcggct gcgacgagtt gtacgccctc gagcgcgagg
     2161 gcaagctcgc ccccttactc gaggcctcgc catgaccgaa gcgcagccac caaactcgcc
     2221 ggcacagccg cagcagccgg cgacgttcca gatcgagaag ctctacgtca aggacctgtc
     2281 gctggaaatc ccgcatgccc cgcaggtatt cgccgagaag gcacagccgc agatcgaagt
     2341 gcacatcaac accggcgaat cgcaattttc cgagggctac tacgaagtga cggtgtcggc
     2401 gacggtcacc gcgcgcgccg gcgagcgcac gctgttcctc gccgaggcgg tgcaggccgg
     2461 catcttcgcg ctgcgcaacg tgcccgcggc cgagctcggc gcgctgctcg gcatcgcctg
     2521 cccgacgatc ctgtttccat acctgcgcga gacgatctcg gatctcgtca tccgcggcgg
     2581 cttcccgccg ctcctgctca gcccgatctc gttcgagcag ctctacctgc agcggctgca
     2641 gcagcaaggc ggcgagggcc cccgcatcga agtggcgcaa taggccatgc gtattgcggt
     2701 gctcggcgca ggcgcctggg gcacctcgat cgcctcggtg ctcggcgcgc gcctcgaggt
     2761 gacgctctgg gcgcgcgacg ccgcccaggc gcgagcgatc gagcagacgc gccgcaacga
     2821 gcgctacctt ccaggcatac cggttcctgc ctcggtggtt gtttcgtcag atctttccaa
     2881 gacggttgcc ggggcgcggc tggtgcttgc cgcgacgccg gtcgccggct tgagagagct
     2941 tcttgggaaa atccgttccg tgacctcgct ggtgtggctg tgcaaggggt tcgaggaagg
     3001 cagcggcgcg ctgccgcacc agattgccgc ggaagtcctg ggcgaggcgg cgcgctgcgg
     3061 cgcgctgtca ggaccgtcct tcgccgaaga agtggcgcgc ggcctgccct gcgcgctgac
     3121 gctcgcctcg cgcgatgccg cgttcgcgcg cgagacggcc gcgctgctgc acggcgggcg
     3181 gctgcgcgtg tattacagcg ccgacctggt gggggtcgag atcggcggcg cggtgaagaa
     3241 cgtgatggcg atcgccgcgg gaatctcgga cggcctcggc ctcggtcaca acgcgcgcgc
     3301 cgcgctgatc acccgcgggc tcgccgagat cgcgcgcctt ggcgccgcgc tcggcggcgc
     3361 gccccagacc ttcatggggc tcgccggcgc cggcgacctg atcctcaccg cgaccggcga
     3421 cctgtcgcgc aaccggcgcg tcggccttga gcttgcgcag ggaagaactt tgaatcaagt
     3481 gctgaaaaat ctaggccacg tcgcggaagg cgtgcgctcg gcgaaggaag tggcgcgcct
     3541 ggctgccgcg cgcggcgtcg acatgccggt gaccgatgcg gtgaacgccg tgcttgccgg
     3601 caagctcacg cccgccgcgg cagtcgagcg tcttctctcg cgcgacccga agaaggagcg
     3661 ctagcccgcg ccgcgaaagc cgagctggcg ccaggcctcg aacaccacca cggctacggc
     3721 gttcgacagg ttcaggctgc ggttccccgg cagcagcggc agcctcaggc gcatttcagt
     3781 ttcaaattga tccagcagct cgtgcggcaa gcctgcggtt tccgcaccga agacgaagac
     3841 atcgtcgtcg gcgaatttca cttcggcgta atttcttgcc gctcgggtcg tcagcgcaaa
     3901 catgcggcgg ccgccgaggg catcgcggca ggcgttccag tcagcgtgaa ctgtgagcga
     3961 agcgagagcg tgatagtcga gcccggcgcg cttgagctgc ttgtcgtcca gagaaaaccc
     4021 gagcggccgg acgaggtgca ggcgcgcgcc ggtattggcg gcgaggcgga tgacgttgcc
     4081 ggtgttcggc ggaatctccg gatgcacgag gacgatgtca aacatcggcc ggcgtgcggc
     4141 acacgaccca gttgaccacg tgcgccgcgc cggagccctt cagcgtgcgc gccatctcgt
     4201 cgagcgtcgc gccagtggtc atgacgtcgt cgagcaccgc gacgcgcgcg ccggcgagcg
     4261 agcgcgtgca gcggaacgcg ccgcgcacgt tgcgctggcg ctcctccagg ggcaggccgg
     4321 tctgcggcgg cgcgttgtcc tgccgcacgc aggccgccag atcgattttc ccgggcttga
     4381 cgtggcgcgc gatctcgagc gcctggttgt agccgcgttc gcgcagccgg tgcggggaga
     4441 gcggcacggg gatgacgcag tccacggcct gatctgccgg aattttttgc agcagcaaat
     4501 ttccgaacag tgaaaccaat gcgagctccc cccggaactt cagcgcgtgc accagcgcgt
     4561 cggctgggaa gcggtaggcg agcgccgcga cggtggcgtc gtagtgcggc gcctcggcga
     4621 ggcagcggcc gcacagctcg cccgagggcg agccaagcgc gcagcgcggg cacagcgggc
     4681 cagcgaggcg cggcagatcg gcatcgcagg ccgcgcagag cggagcgcgc gccgcgcccc
     4741 ggcacaggaa gcaggtgccg ccgaaaagcg cggctgcgag cttggcgtgc atgctatcgt
     4801 ctccgcatgt ggactgtagc gcagctcgcc gccgacctgg catcgggcgc caccaccagc
     4861 cgcgagctcg tcgagcaggc gttcacgcgc atcgtcgacc cggccggcga gggcgcgcgc
     4921 gccttcgtca agatgtacgc cgactcggcg cgcgccgagg ccgacttctc cgaccggctg
     4981 cgccacgcgg gcgtgctgcg ctcgccggtc gacggcctgc cggtgtcggt gaaggacctg
     5041 ttcgacgtcg cgggggacgt gacgcgcgcc ggatcgagaa ttcttgaaac agcttcgccg
     5101 gcgaaagcag acgcggcggc cgtcgcccgg ctgcgcgccg cgggcacggt gctcatcggc
     5161 cgcaccaaca tggtcgagtt cgctttcggc ggcgtcggat tgaatccgca tttcggcact
     5221 ccaaagaacc cctacgacag gaaaacgggg cgcgttcccg gaggctcgtc ttccggcgcc
     5281 gcggtggcgc aagccgatgg catgtgcgtg atggcgctcg gctccgacac gcgcggctcg
     5341 atccgccagc cggcggcgct gtgcggcgtc gcgggattca agcccacggc gcggcgcgtg
     5401 ccgcgcgaag gcgcctttcc gctttccttc acgctcgact ccatcgggcc gctagccaac
     5461 tcggcggcct gctgcgcgac gtacgacgcg attctcgccg gggagccgcc gctgccgctg
     5521 ccggagatgc cggccaaggg cctgaggctc ctcctgcctc gctcctcggc gctcgaggat
     5581 ctggatcctc aggtggggaa ggcattcgac gtcgccctgg cacgtctttc cagggcggga
     5641 atgctggtga ttgaaattcc agtaccagcc ttcgaccggc agaac
//
LOCUS       DASPXW010000041         2138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_769974, whole genome shotgun sequence.
ACCESSION   DASPXW010000041 DASPXW010000000
VERSION     DASPXW010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769974"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..155)
                     /locus_tag="VEL04_00930"
     CDS             complement(<1..155)
                     /locus_tag="VEL04_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HYT46958.1"
                     /translation="MRFIVLVKASKDSEAGVLPTQEMLTEMGKFNDELVKAGVMLAGE
                     GLQDSSKG"
     gene            307..573
                     /locus_tag="VEL04_00935"
     CDS             307..573
                     /locus_tag="VEL04_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HYT46959.1"
                     /translation="MCRNIKTLANFEPPATNDEIRASALQFVRKLSGTTHPSHANEAA
                     FNSAVDDVTTIARRLIDSLSIKAPPRNREEEARKARERAAKRFG"
     gene            619..700
                     /locus_tag="VEL04_00940"
     tRNA            619..700
                     /locus_tag="VEL04_00940"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:653..655,aa:Leu,seq:gag)
     gene            1023..2024
                     /locus_tag="VEL04_00945"
     CDS             1023..2024
                     /locus_tag="VEL04_00945"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYT46960.1"
                     /translation="MLHGSPLDPERARALVLLHGEVRRAAEITSHFLELAAHPAGAAE
                     PCDAAVVINGVLAERESTFRELGVKVIRSIPNDLPMVACPMAQLTEMLTKLLDFSLRR
                     LRAAQPPRELRLEVIATGPSVVASMWDSGTPLAVEAEQRLTSPFRFTQSGGGGEVEFA
                     LARALAQSAGGSLRLRPRGGSAGAEVVATLPRSVLGAQTPFAAPAESLPRMRILVVDD
                     DAVNRQAMVLLLEREGHQIVAVENGLEAMERLGDRDNTFDAIVTDLQMPRLGGRAMYE
                     QLVTSRPLVARRFVFVTGDQARGETRSFLENCGQPSVTKPYDLGDLINAIGTVSRKK"
BASE COUNT          364 a          693 c          698 g          383 t
ORIGIN      
        1 cccttggacg agtcctgcag cccctcacct gccaacatca cccccgcctt caccagctcg
       61 tcgttgaact tgcccatctc ggtcagcatc tcctgcgtcg gcagcacgcc ggcttcggag
      121 tctttgctgg ccttcaccag cacgatgaat cgcatggccg tctcgcttta tagaggttca
      181 ggtctgcgct actttcactt aggcgtcgaa cgagcttccc ggaaatcgac acgccgtaat
      241 cccattgtgg taggcctact tagcggatag actcgggcac gacctttgcg atgatcagga
      301 gccgttatgt gcagaaacat caagacgctc gccaacttcg agccgcctgc gaccaacgat
      361 gagattcgcg cgtcggcgct gcaattcgtg cgcaagctga gcggcacgac gcatccctcg
      421 cacgcgaacg aggcggcttt caacagcgcg gtagatgatg tgacaacgat tgcccggcgg
      481 ctcattgatt ccctgtcgat caaggcccca cctcggaacc gggaggagga ggcgcgcaag
      541 gcgcgggaac gcgcggcgaa gcgatttggc taaggaccgc ctgtacttca tggcggaacg
      601 tttctatgtt tctggcccgc cctggtggtg gaactggtag acacgctgtc ttgagggggc
      661 agtgccgcaa ggcgtctcgg ttcgaatccg agccagggca tcgcgtgacg ggggtcttgt
      721 tgtagtcgtg acgccgggct gctcgcgcaa attcgcgcgg tgcatagctt tccggcatgg
      781 agctatgggt cgccttcgga tttggtgcgg cggtcggcgc actggtcggc tacctgctgg
      841 gacgtctgag ggaggtccca acagcagcgc gggcccgccc tgcgcccccc tccccacctc
      901 caccattgag atcctccagc ccggcctcgc cggacgttaa gcacgtcgtt ctcgacgaag
      961 gctctgccaa cgtattgaac gcgctgaaca accgtctctc gggcatcggc gcgctcgccg
     1021 acttgctgca cggcagccca ctcgaccccg aacgcgcccg cgcactggtg ctgttgcatg
     1081 gcgaggtgcg gcgtgccgcc gaaatcacca gccacttcct cgagctcgcc gcgcatccgg
     1141 cgggggctgc cgaaccgtgc gatgcggcgg tcgtgatcaa cggcgtcctg gcggagcgcg
     1201 aatccacgtt ccgcgagctg ggcgtcaaag tgattcgatc gatccccaac gacctgccca
     1261 tggtcgcctg cccgatggcg cagctcaccg agatgttgac gaagctgctg gatttctcgc
     1321 tgcgccgctt gcgcgcggcg cagccaccgc gagaactgcg actggaggta atcgcgacgg
     1381 gaccttcggt ggtggcatcg atgtgggatt cggggacgcc tttggccgtc gaagcagagc
     1441 agcggctcac gtcgccgttc cgctttacgc agtccggcgg cggcggtgaa gtggagttcg
     1501 cgctcgcccg cgcgctcgcg cagtcggccg gaggcagcct gcggctcagg cctcggggcg
     1561 gcagcgccgg cgccgaagtt gtggcgacat tgccgcggag cgtgctgggt gcgcagaccc
     1621 cgttcgccgc gcccgccgag agcctgccgc gcatgcgcat cctcgtggtg gacgacgacg
     1681 ccgtgaaccg gcaagcgatg gtgctgctgc tcgagcgaga agggcaccag atcgtcgctg
     1741 tcgagaatgg gctcgaggcg atggagcgcc tgggcgatcg cgacaacacg ttcgacgcta
     1801 ttgtcaccga cctgcagatg ccgcggctcg gcgggcgggc aatgtacgag cagctcgtca
     1861 ccagccggcc gctcgtcgcg aggcgattcg tgttcgtcac cggagaccag gcccgcggcg
     1921 agacgcgctc gttcctggag aattgcggac agccgtcggt gacgaagccc tatgatttag
     1981 gggatttgat caacgcaatc gggacagtgt cgagaaaaaa gtagactagt tcggcgcgtg
     2041 caccctcggc ccgccgtaat cgcgatcgcc gccgatgccc ggaccggcga gctcgagccc
     2101 accccgatca ttatcgatcc gcgcgatgaa ctgacgaa
//
LOCUS       DASPXW010000042         2597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1401920, whole genome shotgun sequence.
ACCESSION   DASPXW010000042 DASPXW010000000
VERSION     DASPXW010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1401920"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="VEL04_00950"
     CDS             complement(<1..630)
                     /locus_tag="VEL04_00950"
                     /inference="COORDINATES: protein motif:HMM:NF023222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HYT46961.1"
                     /translation="MLKTGAEHLKQLRDGRVVYIGSERVDDVTTHPGFRNAARSIAAI
                     YDMKAADREFSFADKGERYSAYFLRAKTKDDLLKRTNLHSAIAAMSYGLLGRSPDHVS
                     SFVTGMAMHPSVFGKFSENLVRYYEHMRREDVYGVYAVLPPQAARNPDFYQKQNLPVP
                     SLRVVREDDDGVVISGMKMLATGAVFANEIWIGNLLPLAPTQLAESVTCA"
     gene            complement(611..1021)
                     /locus_tag="VEL04_00955"
     CDS             complement(611..1021)
                     /locus_tag="VEL04_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011548302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HYT46962.1"
                     /translation="MKPKSHLEFVKVDLQTGWERPPGYPPGFFQKILATDLDEARKKG
                     SRTRLLKIEPGAFSAEPFVHDHWEEVYVVQGDLVVGNDQQGRGGEQCFAPTYACRPPG
                     VHHGPFTSRGGCILHEIHYYETSGKEAGHAKDRR"
     gene            complement(1018..1362)
                     /locus_tag="VEL04_00960"
     CDS             complement(1018..1362)
                     /locus_tag="VEL04_00960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HYT46963.1"
                     /translation="MSVSYFVRYEIAVTELKGFLERYRKLHVPAVTCWPGLRRMVMHT
                     PADWQDPFPVNRGKAVLMAQLEFDSEQAMNRAFASPERAAAREDFKRFMTYEGTVTHQ
                     AMGSEEVWRRAK"
     gene            complement(1359..1895)
                     /locus_tag="VEL04_00965"
     CDS             complement(1359..1895)
                     /locus_tag="VEL04_00965"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HYT46964.1"
                     /translation="MRKSRKRAVPLTVSRPALLVKSSDAEFRRLIHDLIAYGHRLDAC
                     RDAFAAIVGISGVQYEILMLVSRAEGLAIGEVAARLHRSGAFITIEANKLVERGILQK
                     ASDPADGRRVLLKNTSKSLQLLERLAPHQRRINDVLFDCLDAKRFGQLRELAGDLLAC
                     GDRAVALLEFLMHEAEAA"
     gene            1960..2307
                     /locus_tag="VEL04_00970"
     CDS             1960..2307
                     /locus_tag="VEL04_00970"
                     /inference="COORDINATES: protein motif:HMM:NF014687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HYT46965.1"
                     /translation="MSDLNDPRVLFAAERTLLAWGRTAAGLMAFGFLIERAGLLVQGK
                     AAAREFGLWIGIGFVLLGVLLSGLSVVQYRRSIAGLRPVEIPPGYMVNLSVFMSIAIG
                     FLGLALAAYMALS"
     gene            2304..2567
                     /locus_tag="VEL04_00975"
     CDS             2304..2567
                     /locus_tag="VEL04_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT46966.1"
                     /translation="MKVDRKGLRAALAHLERGDWRAAHEIVQQDEASPLACWAHGIVH
                     LMEGDLPNARYWYREAKRAFPGKAGATAEIAALRRSLQEKTGG"
BASE COUNT          426 a          865 c          841 g          465 t
ORIGIN      
        1 ggcgcaggtc acggactcgg cgagctgggt gggcgccagc ggcagcaggt tgccgatcca
       61 gatctcgttg gcgaacaccg cgccggtggc gagcatcttc atgcccgaga tcaccacgcc
      121 gtcgtcgtcc tcgcgcacca cgcgcagcga cggcaccggg aggttctgct tctggtagaa
      181 gtccgggttg cgcgccgcct gcggcgggag gaccgcgtac acgccgtaca cgtcttcgcg
      241 gcgcatgtgc tcgtaatacc gaaccagatt ttcagagaat tttccaaaca cggaaggatg
      301 catcgccata cccgtcacaa agctcgagac atggtcgggc gagcgcccga gcaggccgta
      361 gctcatcgca gcgatcgcgg aatggagatt tgttctcttg agaagatcat ccttcgtctt
      421 ggcgcgcagg aagtaggcgc tatagcgctc gcctttgtcg gcgaaactga actcccgatc
      481 agccgccttc atgtcgtaga tcgccgcgat gctgcgcgcg gcgttgcgga aaccagggtg
      541 cgtggtgacg tcgtccaccc tttcggagcc gatatagacg acgcgcccgt ccctcagctg
      601 cttcagatgc tcagcgccgg tctttagcat ggcccgcctc tttccccgag gtctcgtaat
      661 agtggatttc atggaggatg cagccgccgc gcgaggtgaa cggaccgtgg tgcacgcccg
      721 gcggccggca ggcataggtc ggcgcgaagc actgctcgcc gcccctgccc tgctggtcgt
      781 tgccgaccac caggtcgccc tgcaccacgt acacctcttc ccagtggtcg tggacgaacg
      841 gctcggcgga gaacgcgccc ggctcgatct tcaggagccg cgtgcggctg cccttcttcc
      901 gggcttcatc gagatccgtc gccaggatct tctggaaaaa gcccggcgga tatcccggcg
      961 gccgctccca tccggtctgc agatcgacct tcacgaactc aagatgcgac ttgggtttca
     1021 tttcgccctc ctccagactt cctcgctgcc catcgcctga tgcgttaccg tcccctcgta
     1081 cgtcatgaag cgcttgaaat cctctctcgc cgccgcacgc tcgggcgacg caaaagcccg
     1141 gttcattgcc tgttcgctgt cgaattcgag ctgcgccatc agcaccgcct tgccgcggtt
     1201 caccggaaac ggatcttgcc agtcggccgg cgtgtgcatc accatgcgcc gcaggccggg
     1261 ccagcaggta acggccggca cgtggagctt ccggtagcgc tcgagaaagc ccttcagttc
     1321 ggtcaccgcg atctcatagc gcacgaaata ggaaacgctc atgcggcctc cgcctcgtgc
     1381 atcaggaact cgagcagcgc cacggcgcgg tcgccgcacg cgagcagatc gccggccagc
     1441 tcgcgcagct ggccgaagcg cttcgcgtcg aggcaatcga acagcacgtc attgatgcgc
     1501 cgctggtgag gcgccagcct ttccagcagc tgcagacttt tggaagtatt tttcagcaaa
     1561 acccggcggc catccgccgg atccgaggcc ttctgcagaa tcccccgctc gaccagcttg
     1621 ttcgcctcga tggtgatgaa ggcgcccgag cggtgcaggc gcgccgcgac ctcgccgatg
     1681 gcgaggcctt cggcgcggct caccagcatc aggatctcgt actgcacgcc ggagatgccg
     1741 acgatggcgg cgaaggcgtc gcggcaggcg tcgagccggt gcccgtaggc gatcaggtcg
     1801 tggatcaggc ggcggaactc ggcgtcggag ctcttcacca gcagcgccgg gcgcgaaacg
     1861 gtgagcggca cggcgcgttt tctcgatttg cgcattgact gcggtgattt tcggcccgac
     1921 aattagcttt gtcaattagc tttgtacgca aggaggagca tgagcgacct caacgacccg
     1981 cgcgtcctgt tcgccgccga gcgcacgctg ctcgcctggg gccgcaccgc cgccggcctg
     2041 atggcgttcg gcttcctcat cgagcgcgcc ggcctgctgg tccagggcaa ggccgcggcc
     2101 cgggaattcg gcctgtggat cggcatcggc ttcgtgctgc tcggcgtgct gctgtccggg
     2161 ctctcggtcg tgcagtaccg ccgctcgatc gccgggctgc ggccggtcga gatcccgccg
     2221 ggctacatgg tcaacctgtc ggtgttcatg agcatcgcga tcggcttcct cgggctggcg
     2281 ctcgccgcct acatggcgtt gtcttgaagg tcgaccgcaa aggcctgcgc gcggcgctgg
     2341 ctcatctcga gcgcggcgac tggcgcgcgg cgcacgagat cgtgcagcag gacgaggcat
     2401 cgccgctcgc ctgctgggcg cacggcatcg tgcacctgat ggaaggcgat ctgccgaacg
     2461 cgcgctactg gtaccgcgaa gcgaagcgcg cctttccagg gaaagcaggc gccacggcgg
     2521 aaatcgcggc gctgagacga tccctgcagg aaaagaccgg gggctgagcc cggttactgt
     2581 cggcgcgaag cccggcg
//
LOCUS       DASPXW010000043         2655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_13952, whole genome shotgun sequence.
ACCESSION   DASPXW010000043 DASPXW010000000
VERSION     DASPXW010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13952"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..521
                     /locus_tag="VEL04_00980"
     CDS             171..521
                     /locus_tag="VEL04_00980"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HYT46967.1"
                     /translation="MCTPRDVALENDLAYARYESNSLSRGHVIVVPKRHVADFFEMDE
                     AEQAAVLQLLNRARHFIQKNHSPDGYNIGANIGKAAGQSRMHVHLHLIPRYQGDVPDP
                     RGGIRCVLQAARAP"
     gene            complement(508..1236)
                     /locus_tag="VEL04_00985"
     CDS             complement(508..1236)
                     /locus_tag="VEL04_00985"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HYT46968.1"
                     /translation="MVERLITELVIANRILADQDVVDAYGHVSVRHADDPGRYLLACS
                     RSPALVERADIMEFDLEGRPLNPDGRPLYLERFIHGAIYEARPDVLAVVHAHAEDVLP
                     FGITAVPLRPVIHSGAFIGAEVPVWDIAERFGERTNLLVTNMAQGRDLARRLASHNVA
                     LMRGHGFAAAARSLIDVVRMSVYLPRNARVQLKAMQLGEPKPLSRGEIEARTSGYQPQ
                     SPETQRAWQYWAARAGCAELLKEP"
     gene            complement(1221..2618)
                     /locus_tag="VEL04_00990"
     CDS             complement(1221..2618)
                     /locus_tag="VEL04_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HYT46969.1"
                     /translation="MRPIYLTSDIRRIEQAAGEVAPPLMERAGTAAAELAAALASDKS
                     KDILVLAGPGNNGGDARVAAARLKEQFFRVTVISSPKELSEKPWSLVIDGLFGIGLAR
                     EVSGDYAQLIDYANRQACPVLALDMPSGIDSDTGRVLGCAVRASHTISFIALKPGLLT
                     LDGPDHCGEVSVADLGLDLKSLLPASAWVASPGLFKGMLKPRPRNFHKGMAGSLAILG
                     GAAGMTGAALLAGRAALKLGAGRVYVGLLEENSPSADLGALELMLRHPDDALGEDLDA
                     LVVGPGIGDGERAGTLVGAALASDIPCVLDADALNLVSENSDLRKACARRSAETLVTP
                     HPAEAARLLGSTVAAVQADRVKAAKLIAGNLNASAVLKGNGSIVVARDGHWFINASGN
                     AGMASAGMGDVLAGMLGALLAQRYTGETALVLGVHLHGAAADELVRGGTGPVGLTASE
                     VSDAARRLWNSWLSA"
BASE COUNT          406 a          939 c          910 g          400 t
ORIGIN      
        1 aagtactacg tgcacccggc gctgctgcag gcctacctga tggggctgat ggtgcccacg
       61 ccgccgccgc tctcacgccg cgcgcgtcgc aagcagcccg gcgccgcgct gcggcgcgac
      121 gaggtgctgg tgctgcagtt cctgcaggag cgtgtctgag cgcctgcgag ctgtgcacgc
      181 cgcgcgacgt cgcgctcgag aatgatctcg cctatgcgcg ctacgaaagc aattcgctca
      241 gccgcggcca cgtcatcgtg gtgccgaagc gccatgtggc cgatttcttc gagatggacg
      301 aggcggaaca ggccgcggta ctgcagcttc tgaaccgcgc acggcatttc attcaaaaaa
      361 accactcgcc agacggctac aacatcggcg cgaacatcgg caaagccgcg gggcagtcac
      421 gcatgcacgt ccacctgcat ctcattccgc gttaccaggg cgatgtgccc gatccgcgcg
      481 gcggcatacg ctgcgttctg caggccgcta gggctccttg aggagctcgg cgcagccggc
      541 gcgcgcggcc cagtactgcc aggcgcgctg cgtctcgggc gactgcggct ggtagccgct
      601 ggtgcgcgcc tcgatctcgc cgcgcgacag cggcttcggc tcgccgagct gcatcgcctt
      661 gagctgcacg cgcgcgttac gcggcaggta gacggacatg cgcaccacgt cgatgagcga
      721 gcgcgccgcg gcggcgaagc cgtggccgcg catcagcgcg acattgtgcg aggcgaggcg
      781 ccgcgccaga tcgcgcccct gcgccatgtt cgtcaccagc aggttggtgc gctcgccgaa
      841 gcgctcggcg atgtcccaca ccggcacctc ggcgccgatg aaggcgccgg agtggatcac
      901 cggccgtagc ggcacggcgg tgatgccgaa gggcagcacg tcctccgcat gcgcatgcac
      961 caccgccagc acgtcgggac gcgcctcgta gatggcgccg tggatgaagc gctcgaggta
     1021 gagcgggcgg ccgtcgggat tgaggggcct gccttccagg tcgaactcca tgatgtcggc
     1081 gcgctcgacc agcgccgggc tgcgcgagca cgcaagcagg tagcgccccg ggtcgtccgc
     1141 atggcgcacg ctgacatggc cataggcatc gaccacgtcc tggtccgcca ggatgcggtt
     1201 ggcgattacc agctcggtga tcaggcgctc aaccatgaat tccacagcct gcgggccgcg
     1261 tcgctgactt cgctcgccgt gaggccgacc ggtccggtcc cgccgcgcac cagctcgtcg
     1321 gcggcagcgc catgcaggtg cacgccgagc accagcgcgg tttcgccggt atagcgctgc
     1381 gcgagcaggg cgccgagcat cccggcgagc acatcgccca tgcccgcgct cgccatgccg
     1441 gcattgcccg atgcgttgat gaaccagtgg ccgtcgcgcg ccacgacgat gctgccgttg
     1501 cctttcagca cggcgctcgc gttcaggttc ccggcgatca gtttcgcggc cttgacgcgg
     1561 tcggcctgca ccgccgcgac cgtcgagccg agcagcctcg cggcctcggc ggggtgcggg
     1621 gtgacgagcg tctcggcgga gcgccgcgcg cacgccttgc gcagatcgct gttctcggaa
     1681 accaggttga gcgcgtcggc atcgagcacg cacggaatgt cgctcgcgag cgccgcgccg
     1741 accagcgtgc cggcgcgctc gccgtcgccg attcccggcc cgacgaccag cgcgtcgagg
     1801 tcttcgccga gcgcgtcgtc cggatggcgc aacatgagct cgagcgcgcc gagatccgcc
     1861 gacggcgagt tctcctccag cagcccaaca tagacgcgcc cggcgccgag cttgagcgcg
     1921 gcgcgtccgg cgagcagcgc cgcgccggtc atgccggctg cgccgccgag gatggcgagc
     1981 gatcccgcca tgcccttgtg gaagttgcgc ggccggggct tgagcatgcc cttgaaaagc
     2041 ccggggctcg cgacccaggc cgacgcaggc agcaagctct tcaaatccaa tcccagatcg
     2101 gcgacgctga cctcgccgca gtggtccggg ccgtcgagcg tcagcaaccc cggcttgagc
     2161 gcgatgaagc tgatggtgtg gctcgcgcgc accgcgcaac cgagcacgcg gccggtatcg
     2221 gagtcgatgc cgctcggcat gtcgagcgcg agcaccgggc aagcctggcg attcgcgtag
     2281 tcgatgagct gcgcgtagtc gcccgaaact tcgcgcgcca gcccgatgcc gaacaggccg
     2341 tcaatcacga ggctccatgg tttttcggaa agttcttttg gacttgaaat caccgtgaca
     2401 cggaagaact gctccttcaa ccgtgccgcc gcgacgcgcg cgtcgccgcc gttgttgccc
     2461 ggcccggcga gcaccagaat gtccttgctc ttgtcggaag cgagcgccgc cgcgagctcc
     2521 gcggccgccg tcccggcgcg ctccatcagc ggcggcgcca cctcgcccgc ggcctgctcg
     2581 atgcgccgga tgtcgctggt gagataaatc ggtctcattt tttggcgacg ccgagcagcg
     2641 cgtcgggcag ccagg
//
LOCUS       DASPXW010000044         6626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_644970, whole genome shotgun sequence.
ACCESSION   DASPXW010000044 DASPXW010000000
VERSION     DASPXW010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6626
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644970"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            208..1038
                     /locus_tag="VEL04_00995"
     CDS             208..1038
                     /locus_tag="VEL04_00995"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYT46970.1"
                     /translation="MLKPLGLTHGHYECRSLDETLPVLTDLLAMTVLERNNGEAVLRH
                     PNTAWRLVVHEGGPNAPDKPHNNHYGVRVAHAPEVDAAHAYLEANKARFGLRRVSPPR
                     SQHFARSVYFEEPGGNTLEIEYYEPQAAAEGRTIARPHWSRELKQSDFPGRGYVPQAL
                     THGTLECDDKEASAAFYSGVLGLQIAGGGRLSVYVKHAATPWYLVVLPIKRRKKFLRP
                     ANRFTLTLASAKEVEQAHAELRSMKAVTEVQPLQRADGGASFLFCDLNRNWWELSAPG
                     "
     gene            1044..1424
                     /locus_tag="VEL04_01000"
     CDS             1044..1424
                     /locus_tag="VEL04_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT46971.1"
                     /translation="MKRLLIIPLLSLCALAEAQTASPAENEAVSGMAPCLVQGLPDDW
                     VRIYMVVELARPGDESGGVRYLVSRRDTPDQAEPYTPCDIRAPARALLAVRAQQSPER
                     SRWTGARLVLHPSGNFELRFDYPQ"
     gene            complement(1428..3128)
                     /locus_tag="VEL04_01005"
     CDS             complement(1428..3128)
                     /locus_tag="VEL04_01005"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HYT46972.1"
                     /translation="MTSGNKKGLARGLTNYGDPAFSLYLRRSFAKSMGYSGEMLARPV
                     VGIADSRSGFNNCHRHFPELIEAVKRGVLAAGALPLEFPTVSLGEVFLSPTSMMFRNL
                     MSIDVEEMIRAQPMDAVVLVGGCDKTVPAQLMGAASADVPAVQLLAGPMMPTSFRGER
                     LGACTDCRRFWALHRAGKVDSHEIESIEGNLATTAGTCAVMGTASTMASIAEALGMAL
                     PGSAAIPAVHADRLRAAEETGRRAAEMTQAPIRPSQVINAKSVENALRVLLAIGGSTN
                     AIVHLTAIAGRAGIKIDLKRLNQLSDSTPVLVDLKPTGQHYMSDLYASGGIGAVLREL
                     KPLLHLECMTVTGFSLKEILEKQPAWVDRAVVKSFDDPLSKQGGLVALFGSLAPNGAI
                     FKRSAADPQLFEKEGRAVVFASLDDLAARIDDPALDAKPEDFLVLQNAGPKSGYAMPE
                     AGYLPIPAKLARAGVKDMVRISDARMSGTAYGTIVLHVSPEAAIGGPLALVKNGDRIR
                     LSVKQRNLQLLVPENELQKRKAAWKPPASMPTRGYAKLYMDHVLQAEHGCDFDFLRKA
                     "
     gene            complement(3112..4212)
                     /locus_tag="VEL04_01010"
     CDS             complement(3112..4212)
                     /locus_tag="VEL04_01010"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005085.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HYT46973.1"
                     /translation="MAPPFHVEHIGSFVRPGRLLEAARARKESRLDETGFREIQDESI
                     RGIVAFQESLGLPSITDGEFRRRSWSAGFIDAVEGFGLRDGSLTFRNDSRVVGVAASP
                     YAKAPLKRRRRIVADDYRFLKSAVKRGVPKVTIASPAVMHYFLGPKAFDGGVYREREA
                     YFADLVRIYREEIADLGKEGCTYLQLDETALPCNCDPHAREDVAARGENPDELTGRYV
                     RLMNDCLEGKPGKLTVAIHLCRGNLKGAWMAEGGYEPIADAIFNQLDVGIYCLEYDTA
                     RAGDFSPLRFVPKGKRVILGLVSTKTPVLEKRDSLKKRIEMAAKHVPLDQLGIGPQCG
                     FSSGGGGGQAVTQDDTRRKLELVMEVARDVWQ"
     gene            complement(4220..4873)
                     /locus_tag="VEL04_01015"
     CDS             complement(4220..4873)
                     /locus_tag="VEL04_01015"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HYT46974.1"
                     /translation="MLVVSVVVSGCASDGGGVGDRPLTKMEGGALLGALGGAVVGAMA
                     YHQNRTKGAVVGAVGGGLAGGAVGAYMDSQRRDLEKNLGREIQAGEARVETLPNHVVR
                     ITMTSHTAFDTDSTQIKPDFHSTMDKLADVMVRYGKTTLTIVGHTDNVGSDQYNQKLS
                     ERRALSVAQYLESRRVNPLRLATSGKGESQPLASNASESGRQANRRVEIYVEPVVEG"
     gene            complement(4891..5913)
                     /locus_tag="VEL04_01020"
     CDS             complement(4891..5913)
                     /locus_tag="VEL04_01020"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HYT46975.1"
                     /translation="MLFPTTLVGSYPQPEWLIDRKKLAGRFPPRVRARELWRIPEALL
                     AEAQDDATLLAIKAQEDAGLDIITDGEIRRESYSNRFATALEGVDIDHPGTALDRSGH
                     PNPVPRIVGKIRRKHPVEVEDLLFLKKHTKKSTKITVPGPFTLSQQAQNDYYKSEEEA
                     AMDYAAAVNAEIRDLLAAGADVVQVDEPYMQARPEKARQFGLKALNRALDGIKGVTAV
                     HICFGYAAIIHQRPSGYSFLPELADCQCKQVSIETAQSNLDCAVLARLPGKKIMVGCI
                     DLSDMSVETPQKVAERVRKALKYVKPDDVIVAPDCGMKYLPREIAFAKMKAMVEGARL
                     LRTEAL"
     gene            complement(5924..>6626)
                     /locus_tag="VEL04_01025"
     CDS             complement(5924..>6626)
                     /locus_tag="VEL04_01025"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HYT46976.1"
                     /translation="GANSFVRRAHGEAFGTTLTHQQNKFAWFGTTRVFDTLTQTFVRS
                     RHGTFNAHHYRHSPEMSTFVVECDAATWERAGFASLNEAATLRYCETVFAGTLGGHRL
                     VSNKSIWRNFPNLRNERWRAGNTVLIGDALRTAHFSIGSGTRLAMEDAIALERALRKH
                     PKDLEKGLMAYEAARRPIVEKFAAAADASGAWYERFPQHMRLEPREFAWSYIQRSGRI
                     DPERLRRISPKFVDG"
BASE COUNT         1076 a         2214 c         2132 g         1204 t
ORIGIN      
        1 gcgcatcgcg cgcgcactgc atcgcgcccg gccggttggc cagcgcgtcg atcacctcca
       61 gcatgctgta gccgggcttg aggtgcccgg cggcgacgag cgcggcgccg atcggatgga
      121 cgcgctcgcc gatcacggcg ccggcctggt tgtcgttgta gtggcagatc ttcatcgccg
      181 ccctccagtc attgtaagct tggccgcatg ctcaagccgc tcggactgac gcacggacat
      241 tacgaatgcc gctcgctgga cgagacgctt ccggtgttga ccgatctgct cgccatgacg
      301 gtgctcgagc gcaacaacgg agaggccgtc ctcaggcatc cgaacacggc atggcggctg
      361 gtggtgcacg agggcggccc gaatgcgccg gacaagccgc acaacaacca ttacggcgtg
      421 cgcgtcgcgc atgcgccaga ggtcgatgcg gcgcatgcct acctcgaggc gaacaaggcg
      481 cgctttgggc tgcggcgcgt ttcccctccc cgcagccagc acttcgcgcg ctcggtgtat
      541 ttcgaggagc cgggcggcaa cacgctcgag atcgagtact acgagccgca ggccgcggcc
      601 gagggccgca ccatcgcgcg gccgcactgg agccgcgagc tgaagcagag cgattttccg
      661 ggacggggtt atgtgccgca ggcgctgacg cacggcacgc tcgagtgcga cgacaaggaa
      721 gcgagcgccg cgttctatag cggcgtgctc ggcctccaga tcgccggcgg cgggaggctg
      781 tcggtgtacg taaagcacgc cgcgacgcca tggtacctcg tggtgctgcc gatcaagcgg
      841 cgcaagaagt tcctgaggcc ggccaaccgg ttcacgctga cgctcgcttc ggcgaaggag
      901 gtcgagcagg cgcacgccga gctgcgctcg atgaaagccg ttacggaagt tcagccgctc
      961 cagcgagccg atggcggcgc gtcgttcctc ttctgcgatc tcaaccgcaa ctggtgggag
     1021 ctcagcgctc ccggatagct cggatgaaac ggctgctaat tattccgctg ctgtcgctgt
     1081 gcgcgctcgc cgaggcgcag accgcatcgc ccgccgaaaa cgaggcggtg tcggggatgg
     1141 cgccctgcct ggtccaaggc ctgcccgacg actgggtgcg catttacatg gtcgtcgagc
     1201 tggcgcggcc gggcgatgaa tcgggcggcg tgcgctacct cgtgtcgcgc cgggacacgc
     1261 cggaccaagc cgagccttac accccctgcg acatccgggc gccggcgcgc gcgctgctcg
     1321 ccgtgcgcgc gcagcagagc cccgagcgca gccgctggac cggcgcgcgc ctggtgctgc
     1381 atcccagcgg caatttcgag ctgcgcttcg actacccgca gtaggcctca ggccttgcgc
     1441 aggaaatcga agtcgcagcc gtgctccgcc tgcagcacgt ggtccatgta gagcttggcg
     1501 tagccgcgcg ttggcatcga ggccggcggc ttccaggctg cctttctctt ctgcaattcg
     1561 ttttccggaa ccaggagctg caaatttctt tgcttgactg aaagacggat gcggtcgccg
     1621 ttcttcacca gcgcaagcgg cccgccgatc gcggcttcgg gcgagacgtg cagcacgatg
     1681 gtgccgtagg cggtgccgct catcctcgcg tcggagatgc gcaccatgtc cttcacgccg
     1741 gcgcgcgcca gcttcgccgg gatcggcagg tagcccgcct cgggcatcgc atagcccgac
     1801 ttcggtccgg cgttctgcag caccaggaaa tcctccggct tcgcgtccag cgccgggtcg
     1861 tcgatgcgcg ccgcgagatc gtcgagcgag gcgaacacca cggcgcggcc ttctttctcg
     1921 aacagctgcg gatcggccgc cgaacgcttg aagatcgccc cattgggagc gagcgagccg
     1981 aacagcgcga ccaggccacc ctgcttcgac agcgggtcat cgaagctctt caccaccgct
     2041 cgatcgaccc atgccggctg tttttccagg atctccttca gggagaaccc cgtgaccgtc
     2101 atgcactcga ggtgcagcag cggcttcagc tcgcgcagca ccgcgccgat accgcccgag
     2161 gcgtagaggt ccgacatgta atgctggccg gtgggcttca ggtcgaccag aacgggcgtg
     2221 gagtccgaaa gctggttcaa tcgctttaaa tctattttta ttcctgccct gcccgcaatc
     2281 gccgtcaagt gcacgatggc attggtcgag ccgccgatgg cgagcagcac gcgcagcgcg
     2341 ttctcgaccg atttcgcgtt gatgacctgc gaaggacgga tcggcgcctg cgtcatttcg
     2401 gcggcgcggc gccccgtctc ttcggcagcc cgcagccggt ccgcatgcac cgcgggtatc
     2461 gccgcgctgc cgggaagcgc catgccgagc gcctcggcga tcgatgccat ggtcgaggcg
     2521 gtgcccatca ccgcgcaggt ccccgcagtg gtcgccaggt tgccttctat gctttcaatt
     2581 tcatgagaat ccacttttcc tgcgcgatgc agggcccaga agcgccggca gtcggtgcag
     2641 gcgccgaggc gctcgccgcg aaacgacgtc ggcatcatcg gcccggcgag cagctgcacc
     2701 gcgggaacgt cggccgaagc cgcccccatg agctgcgccg gcacggtctt gtcgcagccg
     2761 ccgaccagca ccacggcgtc catcggctgg gcgcggatca tctcctcgac gtcgatcgac
     2821 atcaggttgc ggaacatcat cgaggtcggg ctgaggaaca cctcgccgag cgacacggtc
     2881 gggaattcga gcggcagcgc gcctgcggca agcacgccgc gcttcactgc ctcgatgagc
     2941 tctgggaagt gccggtggca gttgttgaag ccgctcctcg agtcggcgat gccgaccacc
     3001 ggccgcgcca gcatctcgcc cgaatagccc atgctcttgg cgaacgagcg gcgcaggtaa
     3061 agcgagaaag cgggatcgcc gtagttcgtc aggcctcgcg ccaggccctt cttattgcca
     3121 gacgtcacgc gccacctcca tgacgagctc gagcttgcgc cgggtatcgt cctgcgtcac
     3181 cgcctgcccg ccgccgccgc cgctcgagaa gccgcactgc ggcccgatgc caagctggtc
     3241 gagaggtacg tgtttggccg ccatctctat cctctttttc agggaatctc ttttctccag
     3301 cacgggcgtc ttcgtgctga ccaagccaag gatcacgcgc tttcctttcg gcacgaagcg
     3361 cagcggcgag aaatcgccgg cgcgggcggt gtcgtactcc aggcaataga tgccgacgtc
     3421 cagctggttg aagatcgcat cggcgatcgg ctcgtagccg ccctcggcca tccaggcgcc
     3481 tttcaggttg ccgcggcaaa ggtggatcgc aaccgtgagc ttgccgggtt ttccctccaa
     3541 gcagtcattc atcagccgga cgtagcgccc ggtgagctcg tcgggattct cgccgcgcgc
     3601 cgcgacgtcc tcgcgcgcat gcgggtcgca gttgcacggc agcgccgtct cgtcgagctg
     3661 cagataggtg cagccctcct ttccgagatc cgcgatctct tcgcggtaga tacgcaccag
     3721 gtccgcgaaa taggcctcgc gctcgcggta gacgccgccg tcgaacgcct tcgggccgag
     3781 aaagtagtgc atcaccgccg gcgaggcgat cgtcaccttc ggcaccccgc gtttcaccgc
     3841 cgacttgaga aagcggtaat cgtccgcgac gatccgccgg cggcgcttga gcggcgcttt
     3901 cgcatacggc gaggcagcga cgccgaccac ccggctgtcg ttacgaaagg tcaaagaccc
     3961 gtcgcgcagg ccgaagccct ccaccgcatc gatgaacccc gccgaccagc tgcggcggcg
     4021 gaactcgccg tcggtgatcg aaggcaggcc gagcgactcc tggaacgcca cgatgccgcg
     4081 gatggattcg tcctgtattt cccgaaagcc tgtttcatcg agacgacttt ccttgcgcgc
     4141 acgcgcggcc tcgagcaggc ggccgggccg cacgaagctg ccgatgtgct cgacatgaaa
     4201 cggcggcgcc atcggccggt cagccctcga ccaccggctc gacgtagatc tcgacgcggc
     4261 ggttcgcctg ccggccgctt tccgaggcgt tgctcgccag cggctggctt tcacccttgc
     4321 cggaggtagc gagccgcagc gggttgacgc gcctggactc cagatattgc gctaccgaca
     4381 gcgcgcgacg ctcggagagc ttctggttgt attgatcgga tccgacattg tcggtgtgcc
     4441 cgacgatcgt cagcgtggtc ttgccgtagc gcaccatcac atcggcgagc ttgtccatgg
     4501 tgctgtgaaa atcgggcttg atctgggtcg agtcggtgtc gaacgcggta tggctggtca
     4561 tggtgattcg caccacgtgg ttcggcaggg tctcgacgcg cgcctcgccc gcctggatct
     4621 cgcggccgag gtttttctcc aggtcgcgcc tctggctgtc catgtaagcg cccaccgcgc
     4681 ccccggcaag accgccgccc acggcgccga cgacggcgcc cttggtgcgg ttctggtggt
     4741 aggccatcgc acccacgacg gcgccgccca gcgcgccgag gagagccccg ccctccatct
     4801 tggtcagcgg gcgatcaccg accccgccgc catcgctcgc gcaaccggaa accacgacac
     4861 tcaccaccag catgcatatc aaaaacgatt tcatagtgcc tccgttctca gaagtcttgc
     4921 gccctctacc atagccttca tcttggcgaa agcgatctcg cgcggcagat atttcatgcc
     4981 gcagtcgggc gccacgatga cgtcatcggg cttcacgtat ttcaacgcct tccttacccg
     5041 ttcggcaact ttttgtggag tctccacgct catgtcggaa aggtcgatgc agccgaccat
     5101 gatcttcttg cccgggagcc tggcgagcac ggcgcaatcg aggttcgact gcgcggtctc
     5161 gatcgaaacc tgcttgcatt ggcagtcggc aagctccgga aggaaggaat accccgacgg
     5221 ccgctggtgg atgatcgccg cgtagccgaa gcagatatgc actgccgtta cccccttgat
     5281 tccgtccagg gcgcggttga gcgccttgag gccgaattgg cgggccttct cgggacgcgc
     5341 ctgcatgtaa ggctcgtcca cctgcaccac gtcggcgccg gcggcgagca ggtccctgat
     5401 ctccgcgttc accgccgcgg cgtagtccat cgccgcttcc tcttccgatt tgtaatagtc
     5461 gttctgcgcc tgctgcgaca gggtgaacgg gccgggaacg gtgattttcg tgctcttttt
     5521 cgtatgcttt ttcaggaaca gcagatcctc gacttcgacc gggtgctttc tgcggatctt
     5581 gccgacgatg cgcggtacgg ggttgggatg tcccgagcgg tcgagcgccg tgcccgggtg
     5641 gtcgatgtcg acgccttcaa gcgcggtggc aaagcggttg gagtagctct cgcggcggat
     5701 ctcgccgtcg gtaatgatgt cgagtcccgc gtcctcctgc gccttgatgg cgagcagcgt
     5761 ggcgtcgtcc tgcgcctcgg cgaggagcgc ttcggggatg cgccatagct cccgcgcgcg
     5821 cacgcgcggc ggaaaccggc cggcgagctt ctttctgtcg atcagccact cgggctgggg
     5881 atagctgccg acgagcgtgg tgggaaacag catgatttta gtttcatcca tcaacaaact
     5941 tgggggaaat ccttctcagt cgttcggggt cgatgcgccc gcttctctga atatagctcc
     6001 aagcgaattc gcgcggctcg aggcgcatgt gctgcgggaa gcgctcgtac cacgcgccgc
     6061 tcgcgtcggc cgccgctgcg aatttttcca caatgggacg gcgggccgct tcgtacgcca
     6121 tcaatccttt ctccaaatcc ttcggatgtt tgcgcagcgc tctttcgagc gcaattgcgt
     6181 cctccatcgc gagccgggtg ccggagccga tggagaaatg cgccgtgcgc agcgcgtcgc
     6241 cgatgagcac ggtattcccc gcccgccacc gctcatttcg cagattcgga aagttccgcc
     6301 agatcgattt gttcgacacg agacggtgcc ctcccagggt cccggcaaat accgtttcgc
     6361 agtaccgcag ggtagcggct tcgttcaatg aagcgaaccc ggcgcgctcc caggtcgcgg
     6421 catcgcactc tacgacgaag gtgctcatct caggcgagtg ccggtagtga tgcgcgttga
     6481 acgtgccgtg ccggctgcgc acgaaggtct gggtcagcgt gtcgaacacc ctggtggtgc
     6541 cgaaccaggc gaatttgttc tgctggtggg tgagcgtcgt gccgaacgcc tcgccatgcg
     6601 cgcgccgcac gaacgaattg gcgccg
//
LOCUS       DASPXW010000045         3355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1150044, whole genome shotgun sequence.
ACCESSION   DASPXW010000045 DASPXW010000000
VERSION     DASPXW010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150044"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VEL04_01030"
     CDS             complement(<1..319)
                     /locus_tag="VEL04_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HYT46977.1"
                     /translation="MEATANLEALSEDTGTVTLHHTPRRNIAKVPTKCSSCNLRELCL
                     PCGLTGQDADRVDALVYTRRRVKRGESLYRAGDEFSSLYAVRSGFFKTVQTLEDGRDQ
                     VTGF"
     gene            complement(342..752)
                     /locus_tag="VEL04_01035"
     CDS             complement(342..752)
                     /locus_tag="VEL04_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYT46978.1"
                     /translation="MENTPSEPPTNVFIVEDSAPIRERLIGLLGDIEGVTVVGEADTA
                     QAAVEGILRTKPDSVVLDIQLSGGSGLEVLRKIHPLAPEVVFIVLTNHSNPQYRRICT
                     EAGASHFLDKSSEFARVMKIIAGLVGAEQPRLRN"
     gene            complement(883..1536)
                     /locus_tag="VEL04_01040"
     CDS             complement(883..1536)
                     /locus_tag="VEL04_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYT46979.1"
                     /translation="MIRIVVADDHTIVREGLKQVLSAASDLSIVGEAQNGHEVLQRVR
                     ELEFDVLLLDMSMPGKSGIELIKQVRAEKPKLRVLVLSMHQEQQYAVRAIKAGASGYL
                     TKESASAQLVSAIRKVAGGGAFITEAVAEQLALGAMPQADGPPHSALSDREFQVFRLL
                     VSGKAVSSIAGELNLSVKTVSTHKARIMQKMNMSNPTELIRYALQHRLIDDPDSGNA"
     gene            complement(1533..2834)
                     /locus_tag="VEL04_01045"
     CDS             complement(1533..2834)
                     /locus_tag="VEL04_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HYT46980.1"
                     /translation="MTPLEDRGRGRTELLLIVFGPVLALGAFTALLALLASLFGWHPD
                     QMLVTAAGVGAAAVLLALALAYRQVARREAAEGALHNIETRVTGIVESAMDAIITVDE
                     NQHVVLFNAAAESLFGCPRKQAIGAPLAWFIPERFRAEHERHIRGFGETGTSSRRMGG
                     ARIVTGLRRNGEEFPIDASISQITESGKKFYTVILRDVTERVRAEEALRRSQSELREL
                     AYAMNSVREQEKRRIARELHDELGQALTALKLDVAWLSERLPAGDTAIAEKLNAMQAM
                     IDGTVAATRRISSDLRPLILDDLGLVPAAEWLVQEFTQRTGIPCELVVRSPNLELKDP
                     HATAIFRILQESLTNVARHAKAQRVEVALGRSDGTVTLNVRDNGRGFNLDDPRRPNAF
                     GLMGLRERVSLLGGDVRIESQPGQGTVLHVQIPLEEEAQTR"
     gene            2954..>3355
                     /locus_tag="VEL04_01050"
     CDS             2954..>3355
                     /locus_tag="VEL04_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HYT46981.1"
                     /translation="MRAGLVIDSDLIRKYGGSGPRYTSYPTADRFVEAFDAAAYRHWL
                     QNRNIGGFARPLGLYVHLPFCGTICYYCACNKIITKDHGRSAKYLKYLEREARVVAEC
                     LGKDRAIGQTHWGGGTPTFLSDKELGDLTRML"
BASE COUNT          562 a         1081 c         1075 g          637 t
ORIGIN      
        1 ggaagcccgt gacctggtcg cgcccgtcct cgagcgtctg cacggtcttg aagaaaccgc
       61 tgcgcacggc gtagagcgag ctgaattcgt ccccggcacg gtaaaggctt tcgccgcgct
      121 tgacgcgccg gcgcgtgtag accagcgcgt ccacccggtc cgcgtcctgc ccggtgagac
      181 cgcagggcag gcacagttcg cgcagattgc agctcgagca cttcgttgga accttggcga
      241 tgttccgccg gggcgtgtgg tgcagcgtca cggttccggt atcctccgag agcgcttcca
      301 ggtttgctgt agcttccatg gcaattctcc gtcttggtgc ttcaatttct caggcgcggt
      361 tgctctgcgc caaccagtcc tgcgatgatt ttcatgactc tcgcgaattc actgctcttg
      421 tcgagaaagt gactcgcgcc cgcttccgtg cagatgcgtc ggtattgggg gttggagtgg
      481 ttcgtcagga ctatgaaaac cacctccggc gcgagcggat ggatcttgcg caatacctcg
      541 aggccgctgc cgccgctcag ctgaatgtcc agcacgaccg aatccggctt cgtccgcaga
      601 atcccttcga ccgcggcttg cgccgtgtcc gcctcgccga ctaccgtcac gccttcgata
      661 tcgccaagca ggccgataag gcgctcgcgg atcggagccg agtcctctac gatgaagacg
      721 ttcgtgggag gctctgacgg cgtgttttcc atgccgccag tgtgcgagcc ggcatcgccg
      781 ccaactatcg gcggtgcctg agattagccg taggcggctg ctgaaaaacc ttgtaggagt
      841 agcgcggggg cgtctaggaa tcgtcctaca gggaaagaga gctcaggcgt tccccgagtc
      901 cggatcgtcg atcagccggt gctggagggc gtagcggatg agctcggtcg ggttgctcat
      961 gttcatcttc tgcatgatgc gcgccttgtg ggtgctcacc gtcttgaccg agagattcag
     1021 ctcgcccgcg atactcgaca cggccttgcc ggatacgagc agccggaaaa cctgaaactc
     1081 gcggtccgaa agcgcgctgt gcggcgggcc gtcggcctgc ggcatcgccc cgagggcgag
     1141 ttgctcggcc accgcttcgg tgatgaaggc gccgccgccc gccaccttgc ggatcgccga
     1201 tacgagctgc gccgaggcgc tttccttggt aaggtagcct gaggcgcccg ccttgatcgc
     1261 ccggaccgcg tattgctgct cctggtgcat gctgagaacg aggacgcgca gcttgggctt
     1321 ctccgcgcgc acctgcttga tcagctcgat gccgcttttt cccggcatgg acatgtcgag
     1381 cagcagcacg tcgaactcga gctcgcgcac ccgctgcagc acttcgtggc cgttctgcgc
     1441 ctcgcccacg atagagagat cggatgccgc cgagagcacc tgcttcagcc cctcgcgcac
     1501 gatggtatgg tcgtcagcga cgacaatccg gatcatcgcg tctgcgcttc ttcttccagc
     1561 gggatctgca cgtggagaac tgtcccctgc cccggttggc tttcaatgcg gacgtcgcct
     1621 ccgagcaggg acacgcgctc gcgcaggccc atcaagccga aagcattcgg cctgcgcgga
     1681 tcgtcgagat tgaagccgcg gccgttgtcc cggacgttca gcgtcacggt accgtcggag
     1741 cgcccgagcg cgacttcgac gcgctgcgcc ttggcgtgcc gggcgacgtt ggtgagcgac
     1801 tcctgcagga tgcggaatat cgcggtggcg tgcggatcct tgagttcgag attgggcgag
     1861 cggacgacca gctcgcacgg gattcccgtg cgctgggtga attcctgcac cagccactcg
     1921 gcggccggta ccagccccag gtcgtcgagt atcagtgggc gcaaatccga ggagatgcgc
     1981 cgagtcgcgg cgacggtgcc gtcgatcatc gcctgcattg cgttgagctt ctcggcgatc
     2041 gcggtgtccc ctgccgggag tcgctcagag agccaggcga catcgagctt cagggcggtg
     2101 agcgcctgcc ccagctcgtc gtggagttcc cgggcgatgc gcctcttctc ctgttccctg
     2161 accgaattca tggcatacgc gagctcgcgc agctcggact gtgagcgacg cagcgcctct
     2221 tccgcgcgga cccgctcggt gacgtcgcgc agaatcaccg tgtaaaactt ctttccgctt
     2281 tctgtgattt gcgagatcga ggcatcgatc gggaattcct cgccgttgcg cctcaagccg
     2341 gtcacgatgc gcgcgccgcc catccgccgc gaagaagttc cggtttcgcc gaagcccctg
     2401 atgtgtcgtt cgtgctcggc gcggaatcgc tcggggatga accaggcgag cggtgcgccg
     2461 atcgcctgct tgcgcgggca gccgaacagg gactcggccg ccgcgttaaa aagcacgaca
     2521 tgctgatttt cgtcgacggt gatgatggca tccatcgccg attcgacgat gccggtcacc
     2581 cgggtctcga tgttgtgcag cgcaccctcg gcggcctcgc gcctcgcgac ctgccggtac
     2641 gccagcgcga gcgccagcag gaccgccgcg gcgccgacgc ccgcagcggt gaccaacatt
     2701 tgatcgggat gccagccgaa caggctggcg agcagggcga gaagggcggt gaaggcccct
     2761 agcgccagca ccgggccgaa gacgatcagg agcagctcgg tcctgccccg gccgcggtct
     2821 tctaacggag tcatcgagca gcgccctcca cgatcaggcg cacggccttt tcaacgggtt
     2881 ttgacccata tcaacatcgc gggagacaaa tgcttcatat tcaccgggga ataccctgac
     2941 actgaacgca cccgtgcgcg ccggcctcgt catcgattcg gacctgatcc gcaaatacgg
     3001 cgggagcggc ccgcgctaca cttcgtatcc gactgccgac cgcttcgtcg aggcgttcga
     3061 tgcggccgct taccgccatt ggctgcaaaa ccggaatatc ggcggattcg cccgaccgct
     3121 gggattatac gttcacctgc ctttctgcgg gacgatctgc tactactgcg cctgcaacaa
     3181 gatcatcacc aaggaccacg ggagatccgc aaagtatttg aaataccttg agcgcgaggc
     3241 tcgggtggtc gccgagtgtc tggggaagga ccgcgccatc ggccagacgc actggggcgg
     3301 ggggacaccc acgtttctga gcgacaagga gcttggcgat ttgacgcgca tgctg
//
LOCUS       DASPXW010000046         3126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_393562, whole genome shotgun sequence.
ACCESSION   DASPXW010000046 DASPXW010000000
VERSION     DASPXW010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393562"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VEL04_01055"
     CDS             complement(<1..551)
                     /locus_tag="VEL04_01055"
                     /inference="COORDINATES: protein motif:HMM:NF026200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HYT46982.1"
                     /translation="MAADADTAPLFSAPYAPPDEAIAPRLLREATRANDAERRIDAYA
                     RRLIEGIRARAGGLGGIEDFLHAYSLSTKEGLALMVLAEALLRVPDAATADRLIEDKL
                     ATGDWSSHHVKSSALLVSASAWTLGVTARIIHPGETPDTILESLIKRLGIPAVRAATR
                     QAMRLLGSHFVLGQTIEEALRRAG"
     gene            824..1858
                     /locus_tag="VEL04_01060"
     CDS             824..1858
                     /locus_tag="VEL04_01060"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYT46983.1"
                     /translation="MGLLNLRQIVRPAHLFAAAIALMAEGPAAAQTSIKFSLDGRLEG
                     LAATFFFPQDKGYFRSEGLDVTLDEAASVLDPITRVASGAYDMGLADINTLIKYRDQH
                     PSPPVRAVFMLYNKPPFAIVARRSRGITEPKQLENKKLGAPPAGITFGEWPLFAKLNN
                     IDVSKVTIETIGIPVRAPMLAAGQIDAALGYSFRLYVDLKDRGVPVDDIVLMPMADYG
                     LKLYGNTIIINPKFAADKPEAVRAFLHAFLKGLKEIVRRPVDAVDAVVRRDETLKKEV
                     ELERLRMAIRDNIVTPEVKADGYGAVDFARLGESIDQIGLVYTFKAKPKAADIFDASF
                     LPPADERKVH"
     gene            complement(1876..2940)
                     /locus_tag="VEL04_01065"
     CDS             complement(1876..2940)
                     /locus_tag="VEL04_01065"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HYT46984.1"
                     /translation="MLRLTQSQLSTHSRRCEYEVGRRPERGRRGLALRARRRPNAQER
                     RGGMVNIPTELLRTLVFVVDQRSFTKAAQSLGVTQPAVSAQIKRLQFLLGYELLDKSA
                     PGVSLTPRGEMVVAHARRMLSINDQIAQLGGGQPTAQTVRVAMPGDFAGTRIPATLAR
                     FRKRWPGIRFAASATSFENTLRGLRQGDIDLAVAIASSKPAIEAQHLWIDQAVWVRSD
                     ATQLDPVGPVPLVSFGEDCACRYTAVNALHRIGRECDFVFTSRSIASLEAAVLAGLGI
                     MVLPRSRVQQTSLSIWEDAPLPELPQLYCGIYLRQGGNRAALEELAEDIAAVLRPQLQ
                     VKKGGAASDIAPARASGSGQ"
     gene            complement(2928..>3126)
                     /locus_tag="VEL04_01070"
     CDS             complement(2928..>3126)
                     /locus_tag="VEL04_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT46985.1"
                     /translation="LLLATHPRAVIAAADRDRALSRRELSQADETRLCAMIVSHQACL
                     RSIDDTIFADFERADNVYCCA"
BASE COUNT          550 a         1025 c         1013 g          538 t
ORIGIN      
        1 cccgcccgcc gcaacgcctc ctcgatggtc tgtccgagca cgaaatgcga gcccaggagc
       61 cgcatcgcct gccgcgtggc agcgcgcacg gcaggaatgc cgaggcgttt gatgagactt
      121 tcgagaatcg tatccggggt ctcgccggga tggatgatgc gggcggtgac gccgagcgtc
      181 caggccgagg ccgacaccag cagcgcgctc gatttgacgt ggtgcgacga ccagtcgccg
      241 gtggcgagct tgtcttcgat cagccggtcg gcggtcgcgg catcgggcac gcgcagcagc
      301 gcctccgcca acaccatgag ggcgaggccc tccttggtgg acagcgaata ggcatgcagg
      361 aaatcctcga tgccgccgag ccccccggcc ctggcccgga tcccctcgat gaggcgccgc
      421 gcatacgcgt cgattcgccg ctcggcgtcg ttcgcgcggg tggcctcacg gagcagtcgg
      481 ggcgcgatcg cctcgtcggg cggcgcgtag ggagcgctga agaggggtgc tgtgtctgca
      541 tccgcggcca aggatatcgt cgtatctggg atcatgaccg gtccacactc gactggctcc
      601 ggctcgggcc tgcggcgtta atcttaacag ccgttcatgc catcaggctg aagaataact
      661 cgcatgcggg cacaaagtgg cagggagcgg tgtttgaaat ttgtgaaggg cgggctcgaa
      721 ctgcgatctc gccttgcgct ttgcacgctc atgttcttta tctggctgcc tcagccgcgg
      781 tgcagggcgg ggtggcgcgg gggccgacgc agagggagca gccatgggcc tcctcaacct
      841 caggcagatc gttcggcccg cccacctctt cgcggcggcg atcgcattga tggccgaagg
      901 gccggccgcg gcgcagacca gcatcaagtt ctccctcgac gggcgcctcg agggactggc
      961 tgcgactttt ttctttcccc aggacaaggg ctacttcagg agcgagggtc tggacgtgac
     1021 cctcgacgag gcggcgagcg tgctcgaccc gatcacccgc gttgcatcgg gcgcctatga
     1081 catgggcctc gccgatatca ataccctcat caaatatcgc gatcagcatc cgtcgccacc
     1141 ggtgcgagcg gtattcatgc tctacaacaa gccgccattc gcgatcgtgg cgcgcaggag
     1201 ccgcggcatt accgagccca agcagctcga gaacaagaaa ctcggcgccc cacccgccgg
     1261 catcaccttt ggcgagtggc cgctattcgc caagctcaac aacatcgatg tgtcgaaggt
     1321 gacgatcgaa accatcggca taccggtgcg cgcgcccatg ctcgccgccg gccagatcga
     1381 cgccgcgctc ggctactcgt tccggctcta tgtcgatctc aaggaccgcg gcgtgccggt
     1441 cgacgatatc gtgctgatgc cgatggcgga ttacgggctc aagctctatg gcaacaccat
     1501 catcatcaat cccaagtttg cggccgacaa gccggaggcg gtgagagcgt ttctgcacgc
     1561 ctttctcaag ggcctgaagg agatcgtcag acgaccggtc gatgcggtcg atgcggtcgt
     1621 gcggcgcgac gagaccctga aaaaggaggt cgagctcgag cggctgcgca tggccatccg
     1681 cgacaacatc gtcacgccgg aggtgaaagc cgacggctac ggcgccgtcg acttcgcgcg
     1741 gctcggggaa tcgatcgatc agatcgggtt ggtctacacc ttcaaggcca agcccaaggc
     1801 ggccgacatt ttcgacgcct cgttcctgcc gcccgccgac gagcgcaagg tgcattgagt
     1861 tcgttgcaac gtgccttact gccccgatcc gctcgcccgc gcgggagcga tatcgctcgc
     1921 agccccgccc ttcttcacct gcagctgcgg acgcaacacc gccgcgatgt cctcggcgag
     1981 ttcctcgagc gccgcccggt tgccgccttg gcgcaaataa atcccgcaat agagctgggg
     2041 tagctcggga agcggcgcgt cctcccagat cgagagcgac gtctgctgca cgcggctgcg
     2101 cggtagcacc atgatgccga gcccggcgag gaccgcggcc tccaggctcg cgatgctgcg
     2161 cgaggtgaac acgaagtcgc actcgcgccc gatgcgatga agcgcgttca ccgccgtgta
     2221 gcgacaggcg caatcttcgc cgaaggacac gagcggcacc ggcccgaccg ggtcgagttg
     2281 ggtcgcgtcg ctgcgcaccc acaccgcctg gtcgatccac aaatgctgcg cttcgatcgc
     2341 gggcttcgag cttgcaatcg caaccgcaag atcgatgtct ccttggcgca atccgcgcaa
     2401 cgtgttttca aagcttgtcg cgcttgccgc gaaacgaatg cccggccagc gcttgcggaa
     2461 ccgcgccaag gtcgcgggaa tcctggttcc ggcgaagtcg ccgggcatgg cgacgcgcac
     2521 ggtctgcgcc gtcggctgcc cgccgccgag ctgcgcgatc tgatcgttga tcgacagcat
     2581 gcgccgcgcg tgagcgacga ccatctcccc gcgcggggtc aggctcacgc ccggggcgct
     2641 cttgtcgagg agctcgtagc cgagcagaaa ttgcagccgc ttgatctgcg cgctgaccgc
     2701 cggctgggtg acgccgagcg actgcgccgc tttggtaaag ctgcgctgat cgacgacgaa
     2761 cacgagtgtc cgcagaagtt cagtcgggat attgaccatg ccccctcgcc gttcttgcgc
     2821 gttcgggcgc cgccttgccc gcaaggccaa cccccgccga ccccgttcgg gccggcggcc
     2881 gacctcatat tcgcaccgtc gcgaatgtgt gctcaattgc gattgagtca agcgcagcaa
     2941 taaacgttgt cagcacgttc aaaatcggca aatattgtgt catcaatcga tcgtaggcat
     3001 gcctggtgcg atacaatcat tgcgcacaag cgcgtttcat cggcttgcga gagttcccgg
     3061 cgggagagcg cgcgatcccg atcggcagcg gcgatgaccg cgcgtgggtg cgttgcgagc
     3121 aggagg
//
LOCUS       DASPXW010000047         2152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1023975, whole genome shotgun sequence.
ACCESSION   DASPXW010000047 DASPXW010000000
VERSION     DASPXW010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023975"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..849)
                     /locus_tag="VEL04_01075"
     CDS             complement(61..849)
                     /locus_tag="VEL04_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase"
                     /protein_id="HYT46986.1"
                     /translation="MLLESGVDVPLVVTHRDDPGERIWFASVAELARGRGIEVLEDPQ
                     QDELLRKIKAATSDFIFSFYYRRMLAPELLKIAKRGAFNMHGSLLPKYRGRAPVNWAV
                     LNGETETGATLHEMVAKPDAGRIVDQQRVPIGPDDLAVEVFRKVTGAAEIVLQRNIKK
                     ILEGKTTLRPQDLSRGSYFGGRRPEDGRIDWSKSARQIHNLVRAVAPPYPGAFSGKMK
                     IHRTRMEEGKKGPARLGPYNEGGQWFAVCGDGKVLRLLEVQADK"
     gene            complement(867..1844)
                     /locus_tag="VEL04_01080"
     CDS             complement(867..1844)
                     /locus_tag="VEL04_01080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYT46987.1"
                     /translation="MNLSVVIPVYNEAAGLPQLFGRLYPALDGLGVAYELIFVNDGSR
                     DRSAALLREQFAKRPDVTRVVLLNGNFGQHMAIMAGFERCRGERVVTLDADLQNPPEE
                     IGKLLGKMDAGHDYVGGVRRERRDAALRRLLSRLMNRVRERITRIRMTDQGCMLRAYS
                     RDIVEAINACQEVSTFIPALAYTFARNPAEVDVAHEERAAGVSKYPFYRLVRLNFDLI
                     TGFSLVPLQLFSLFGILVSVGAVLVYLVVMVQRILLGEPLNALRTFWDRDLLQFFLIG
                     IVLFGVGLVGEYVGRIYQQVRHRPRYLVRAVLEKDSSWEAKRSSSATTT"
     gene            complement(1841..>2152)
                     /locus_tag="VEL04_01085"
     CDS             complement(1841..>2152)
                     /locus_tag="VEL04_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HYT46988.1"
                     /translation="PPEDFENSNWHMFQVVLPERSERGREPSLRADFIRRMRDAGIGV
                     GVHYPAMHLFALYRRLGWRAGQFPHAERIGRSIVTLPLFSLMQDQDVDRVCDAARKAL
                     K"
BASE COUNT          390 a          696 c          700 g          366 t
ORIGIN      
        1 tggccgatga agccgttgac gcccaggatc aggatccgct tcataaggtc aggatgcgtt
       61 tcatttatcc gcttggacct cgaggaggcg caggacttta ccatcgccgc agacggcgaa
      121 ccattgaccg ccttcgttgt agggaccgag cctcgcaggg cccttctttc cttcctccat
      181 cctggtgcgg tggattttca tttttcctga aaaagcacct gggtaggggg gcgccacggc
      241 gcgcaccagg ttgtggatct ggcgcgcgct cttcgaccag tcgatgcgcc cgtcctcggg
      301 cctgcggccg ccgaagtagc tgccgcgcga caggtcttgc gggcgcaagg ttgtttttcc
      361 ttcaagtatc ttttttatgt tcctctgcaa cacgatttcg gcagcgccgg tcaccttgcg
      421 gaacacctcc accgcgagat cgtccgggcc gatcggcacg cgctgctggt cgacgatgcg
      481 gccggcgtcg ggcttcgcca ccatctcgtg cagcgtcgcg ccggtctcgg tctcgccgtt
      541 gaggactgcc cagttgaccg gggcgcggcc acggtatttc ggcagcagcg agccgtgcat
      601 gttgaacgcg ccgcgcttcg ctatcttgag gagttcggga gccagcatgc ggcggtagta
      661 gaacgagaag atgaagtccg atgtcgccgc tttgatcttt cgcagcaatt catcctgctg
      721 aggatcttcg agaacctcga tgccgcggcc gcgcgccaac tcggcgacgc tcgcgaacca
      781 gatgcgctcg ccgggatcgt cgcggtgcgt gacgacgagg ggcacgtcga cgcccgactc
      841 gagcagcacg cgcaggcagc gtacgcctac gtcgtggtag ccgaagacga ccgctttgct
      901 tcccaggaag aatccttttc cagcactgcc cgaaccaggt agcgcgggcg gtggcgcacc
      961 tgctggtaga tgcgcccgac gtattcgccg accagcccga cgccgaacag cacgatgccg
     1021 atgaggaaga actgcagcag gtcgcgatcc cagaacgtgc gcagcgcgtt gagcggctcg
     1081 ccgagaagga tgcgctggac catgaccacc agatagacca gcaccgcgcc aaccgacacc
     1141 aggataccga acagcgagaa cagctgcagc ggcaccagcg aaaagccggt gatgaggtcg
     1201 aaattgaggc gcaccaggcg gtagaacgga tacttcgaga cgccggctgc gcgctcctcg
     1261 tgcgcgacgt cgacctcggc cggattcctc gcgaaggtat aggcgagcgc ggggatgaag
     1321 gtgcttacct cctggcaggc attgatggcc tcgacgatat ccctgctata ggcgcgcagc
     1381 atgcatccct ggtcggtcat gcggatccgg gtgatgcgct cgcgcacccg gttcatgagg
     1441 cgcgagagca ggcggcgcag cgccgcgtcc cggcgctcgc gccgcacgcc gccgacgtag
     1501 tcgtggcccg cgtccatctt gccgagcagc ttgccgatct cctcgggcgg gttctgcagg
     1561 tcggcgtcga gcgtcacgac gcgctcgccg cggcagcgct cgaagcccgc catgatcgcc
     1621 atgtgctggc cgaaattgcc gttgagcagc accacccggg taacatccgg gcgcttggcg
     1681 aactgctcgc gcagcagcgc cgcggaacgg tcgcggctgc cgtcgttgac gaagatcagc
     1741 tcgtaagcga cgccgagccc atcgagcgcc gggtacaggc ggccgaacag ctgcggcagc
     1801 ccggctgcct cgttgtacac cgggatgacg accgagagat tcatttcagg gccttgcgcg
     1861 cggcatcaca gacacggtcc acatcttgat cttgcatcag agaaaacaaa ggaagagtaa
     1921 cgatcgaacg ccctatgcgc tcggcgtgcg gaaactggcc tgcgcgccag cccaggcgcc
     1981 ggtacagcgc gaacagatgc atcgccgggt agtgcacgcc gacaccgatg ccggcgtcgc
     2041 gcatgcgccg gatgaaatcg gcgcgcaggg aaggctcgcg cccgcgctcg gagcgctccg
     2101 gcagcaccac ctggaacatg tgccagttcg agttctcgaa atcctccggc gg
//
LOCUS       DASPXW010000048         7208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_267302, whole genome shotgun sequence.
ACCESSION   DASPXW010000048 DASPXW010000000
VERSION     DASPXW010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7208
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267302"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..585
                     /locus_tag="VEL04_01090"
     CDS             130..585
                     /locus_tag="VEL04_01090"
                     /inference="COORDINATES: protein motif:HMM:NF018717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HYT46989.1"
                     /translation="MNQSLILTKVPSAVRTIQRGESMKTRYTVTLAAIAGFGLGAIAI
                     EGLHAQAKPPVYYIAEIDVTNLDAYLKEYTPMAQATIKKAGGRVLAAGQKVTAIEGQP
                     PKPRVAVLMWDSMEKMQAWRNLKEFKENRKIGDKYAKFRSFAIEGVPQK"
     gene            complement(651..1088)
                     /locus_tag="VEL04_01095"
     CDS             complement(651..1088)
                     /locus_tag="VEL04_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HYT46990.1"
                     /translation="MHSIKREFVAAAGALLLGVAGYAAGAGPQDKVVYHFSDGLEQAS
                     NGLRNIGNHLEVNPTAKIIVVSHARGVDFLMKGAKDKNGNQYEDLVEQLAIQGVQFDV
                     CEITLRNRRLDKKQFIADVKYVPSGVAEITRLQQREGYAYLKP"
     gene            complement(1105..2214)
                     /locus_tag="VEL04_01100"
     CDS             complement(1105..2214)
                     /locus_tag="VEL04_01100"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HYT46991.1"
                     /translation="MKRWSTIAVVLSFSCMTASAATFVYVSNAEDGDIGTYRMTDTGE
                     LQSGPRAKAASVVMPMAVSPDRRFLYAGVRSKPYSVIAYSIDSGTGELRQLSTSPLAE
                     SFPYISLDKTGRFLFGASYAGHLISVNAVGRDGRVAAEPLQVIPVGRNAHSIRSDESN
                     KFVYVPTLGTDQIFKFVFDARSGRLTSNTPAVFLMKAGAGPRHFITSRDNKFLYALSE
                     LQGTVTTFSLDGKTGLLTEVSVASGLPPDTKLVPGAPRPSSRNTENDVWAADLHMTPD
                     GKLLYMSERTSSSLGAFRVDAETGKLAYLGSTPTEKQPRGFAIDAKGRFLVASGEKSA
                     TLSVYAIDPASGALRPVGQFPAGKGANWVEIVRFD"
     gene            complement(2268..3077)
                     /locus_tag="VEL04_01105"
     CDS             complement(2268..3077)
                     /locus_tag="VEL04_01105"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HYT46992.1"
                     /translation="MRSRRRRFRIAPTRLRARSRRALRVFLAVPLAVQLAIGAFVIVA
                     LWLALNWVYQVVRKPAELLFPVSGVLSKAPAETWRQYESIFREHSTAVVTPDLLAALA
                     QVEGAGNPAARTYWRWRLTQEPFEVYRPASSAVGMYQITDATFREAKRYCIRDHGVVE
                     DGPWHDISSCWFNSLYTRVVPSHAVEMTSAFLDRRVAGILQRRGIAAATLQQKQDLAA
                     LIHLCGATAGDEYAKRGFRLAASQRCGDHDARGYLAQLNAMKRVFSRLAAS"
     gene            complement(3074..4822)
                     /locus_tag="VEL04_01110"
     CDS             complement(3074..4822)
                     /locus_tag="VEL04_01110"
                     /inference="COORDINATES: protein motif:HMM:NF024732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HYT46993.1"
                     /translation="MPLLNALYARVKAQARLHLSIISALSLEVPRASGELERRFLEPY
                     TRRAFAGVPELDYMRDLRSGRLPANVELSEFYFRPGAMLGVPAAQQSYISSNYTHVAR
                     DMRARGVNAVLVMVAERGGRYSLSCNPDLTLDVARAMRAGGRPCVVAGLVNRNLPFML
                     HDAEVGGDYFDVLVDAPRWEHRLFGVPNPAAEPVDHAIGMHAAALVKDGGTLQLGIGS
                     LGDAVAHWLRLRHTDPGSFGAALQALEAEHALIEREGGRGRFAEGLFASSEMFTWGLM
                     TLYRAGVIRRRANGADGPVLQGAFFLGPSEFYRALHELSEEERGLFEMTSVSRINDLY
                     GEEALGRRQRLHARFINICMKVTLFGAAVSDGLADGRVVSGVGGQYNFVAMAHELEGA
                     RSILLLRATRESGGSVESNIVFNYGHETIPRHLRDVVVTQYGIADLRGRTDAEVAAAL
                     IAIADRRFQGGLAARAKAAGKLPRDWRVPERALGNTEERLAARLAPLAGRGLLPMFPL
                     GTDFDADEQRLVRALLWLQKNAAGWRNRLALAGALLGARPQQGDAAALARMSLADPRG
                     VRERLLRRAVALALSR"
     gene            complement(4924..5400)
                     /locus_tag="VEL04_01115"
     CDS             complement(4924..5400)
                     /locus_tag="VEL04_01115"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HYT46994.1"
                     /translation="MRELLPHAERVAALLKERKETVAVAESSAGGLITAALLGVPGAS
                     AYCLGGTVIYTRQAWRSLRDFREDLLEGKRSATEGNALIRARLARERFAADWGIGETG
                     ATGPAGNRYGDPAGHACLAVSGRSERAITLKTGSSDRVRNMYAFAAAALDLLAESL"
     gene            5495..6271
                     /locus_tag="VEL04_01120"
     CDS             5495..6271
                     /locus_tag="VEL04_01120"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HYT46995.1"
                     /translation="MRTNTTLVMNPKGGSGKTTVATNLASYFASNNLATTLMDYDPQG
                     SSLNWLRLRPPHAPRIHGANGAPPKAGRLRSIDMHVPPETRQLIIDAPAGASGLLLQE
                     LLSRAHCILIPVAPSVIDIHATANFIRDLLIAGKIRANNIRLAVVASRVRRSMPVYQP
                     LERFLNSLNLPLLARLGDSDVYVGAAESGIGIFEMDFAASCAERQQFLPIAEWVDSLQ
                     LIVASVQTPLPSRPAPILAPARPGLRVIGNGTSSFPGIRR"
     gene            6326..6937
                     /locus_tag="VEL04_01125"
     CDS             6326..6937
                     /locus_tag="VEL04_01125"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HYT46996.1"
                     /translation="MAVLRIAKMGNPVLSQVAAPVADPTAPGIKKLAADMRDTIEDIG
                     ASGLAAPQVFENKRVVVYRIIASRIPEGSGLEPRPWTVMVNPVIKPKTDQKTLVWERC
                     LSVPGLHGKVPRFSHITISYQDLDGRSASHDASSSWAALLQHECDHLDGIVYPMRMTD
                     LRLLAYNDEPGPLAREVAADPKGIDPLFIDLVERWPGRERWIG"
     gene            6967..>7208
                     /locus_tag="VEL04_01130"
     CDS             6967..>7208
                     /locus_tag="VEL04_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HYT46997.1"
                     /translation="MTENQGVTQMEIDQRVFELYDEYCHGRIDRREFLKRASALSVAG
                     LAMAQALLPRYAQAQTISFTDARIKAQYVIYPSPGG"
BASE COUNT         1272 a         2420 c         2344 g         1172 t
ORIGIN      
        1 cgcggcggcc gccgaccaca acttcgcggc gcctcgcggg cgcctgcgca cgcgccgcct
       61 cgagcaaggc acagcttgtg aaaaccattc ctttgatgaa gtccctgcgt gttgtcaacg
      121 tgttcctcct tgaatcagag cctgatctta actaaagtgc ccagtgcagt acgaaccatc
      181 caaaggggag aaagcatgaa aactcgttat accgttacgc tggcggcaat tgccggtttc
      241 gggctcggcg ctatcgcgat cgaggggctc cacgctcagg ccaagccgcc cgtctactac
      301 atcgcggaga tcgacgtgac caacctcgac gcgtatctga aggaatacac gccgatggcg
      361 caggctacga tcaagaaagc cggcggtcgt gtcctggctg cgggacaaaa agtgacggca
      421 atcgaagggc agccgccgaa accacgcgtc gccgttctga tgtgggacag catggaaaaa
      481 atgcaggcct ggcgcaactt gaaagaattc aaggagaacc gcaagatcgg cgacaagtac
      541 gcgaaattcc gttcatttgc catcgagggc gtgccgcaga agtagcgcag ccgttttatg
      601 ctggaggcag gatcgaggcc gccccgttgg cggcctctct ttttgcagct tcaaggtttg
      661 aggtaagcgt atccctcgcg ctgctgcagg cgggtaatct ccgcgactcc cgacggcacg
      721 tatttcacgt cggcgatgaa ctgcttcttg tcgagcctgc ggttgcgcaa ggtgatctcg
      781 cacacatcga actgcacgcc ctggattgcc agctgctcga cgagatcctc gtactggttg
      841 ccgttcttgt ccttcgcgcc cttcatcaga aaatccacgc cacgggcgtg cgacacgacg
      901 atgattttcg ccgtcggatt cacttcgagg tgattgccaa tgttgcgcag tccgtttgac
      961 gcctgctcga ggccgtcgct gaagtggtac accaccttgt cctgcggtcc tgcgcccgcc
     1021 gcgtagcccg cgacgccgag cagaagggcg cccgctgcgg cgacaaattc ccgcttgatg
     1081 gaatgcatct cgcctccggt tacttcaatc gaacctgacg atttcgaccc agttggcgcc
     1141 tttccctgcc ggaaactggc cgaccggccg aagcgcgcca ctcgccgggt cgatcgcata
     1201 caccgaaagc gtcgccgact tttcgccgct cgcaaccagg aatcgtccct tggcatcgat
     1261 ggcgaagccg cgcggctgct tctcggtagg cgtgcttccc agataggcga gcttgccggt
     1321 ttccgcgtcg acgcggaacg cgcccaggct gctgctggtg cgctcggaca tgtacaacag
     1381 cttgccgtcc ggggtcatgt gaaggtccgc agcccagaca tcgttttcgg tattgcggga
     1441 cgacgggcgt ggcgcgccgg gcacgagttt cgtatcgggt ggcaggcccg acgccacgct
     1501 cacttcggtc agcaatccgg tcttgccgtc cagcgagaag gtggtgactg tgccctgcag
     1561 ctcgctcagc gcgtacagga acttgttgtc gcgcgaagtg atgaagtggc gcgggccggc
     1621 ccctgctttc atgaggaaaa ccgcgggcgt gttcgaggtc agccggccgg acctggcgtc
     1681 gaaaacgaac ttgaagatct ggtcggtgcc gagcgtgggg acatacacga acttgttgct
     1741 ctcgtcgctg cggatcgagt gcgcattgcg ccccaccggg atcacctgca gcggctcagc
     1801 tgcaacgcgc ccatcgcggc ctaccgcgtt gacgctgatg agatgaccgg cataggacgc
     1861 gccgaacagg aaccgtccgg tcttgtcgag cgagatgtac gggaagctct ccgccagcgg
     1921 cgaggtcgac aattgccgca gctcgcccgt gccggagtcg atggaatagg cgatcaccga
     1981 atacggcttg gagcggactc cggcatagag aaagcggcgg tccgggctta cggccatcgg
     2041 catcactacg ctagcggcct tggcgcgcgg cccggactgc agctcgcccg tatcggtcat
     2101 gcgataggta ccgatgtcgc catcctcggc gttcgatacg tagacgaatg ttgcggcgga
     2161 tgccgtcatg caggagaaac ttagcaccac ggcgatagtg gaccagcgtt tcattgtgaa
     2221 ctccccgctt gaccggccgg aaagatgggg cacccagcag tcgactgtca agacgccgcc
     2281 agccgggaaa acacccgctt catcgcattg agctgggcga gatagcctcg cgcgtcgtga
     2341 tcgccgcagc gctggctggc ggccaggcgg aaaccgcgct tcgcgtactc atcgcccgcc
     2401 gtggcgccgc acaggtggat cagcgcagcc aggtcctgct tctgctggag cgtcgcggcg
     2461 gcgatgcccc ggcgctgcag gatgcccgcc acgcggcgat cgaggaatgc cgaggtcatc
     2521 tcgacggcgt ggcttgggac gacgcgtgtg tacaggctgt tgaaccagca cgagctgatg
     2581 tcgtgccagg gaccatcctc gaccacgccg tggtcgcgaa tgcaatagcg cttcgcttcg
     2641 cggaacgtcg cgtcggtgat ctggtacatg ccgaccgcgc tcgacgccgg ccgatagacc
     2701 tcgaagggtt cctgcgtcag gcgccacctc cagtacgtcc gggctgccgg gttgcccgcg
     2761 ccctcgacct gagcgagcgc tgcgaggagg tcaggcgtga cgacggcagt cgaatgctcg
     2821 cgaaagatcg actcgtactg ccgccaggtc tcggccggcg ccttcgagag cacgccgctc
     2881 accggaaaga gcagctccgc gggcttacgg accacctgat acacccagtt gagcgcaagc
     2941 cagagcgcga cgatcacgaa cgcgccgatg gcgagctgaa ccgccagcgg gacggccaga
     3001 aatacccgca gcgcgcggcg cgaccgggca cgcagccgcg tcggtgcgat gcggaagcgg
     3061 cggcgtcgcg acctcacctg gacaatgcca gcgccacggc acggcgcagc agccgctcgc
     3121 ggactccccg cgggtcagcg agactcatgc gcgcgagcgc cgcagcgtcc ccctgctgcg
     3181 gccgcgcgcc gagcagcgcg ccggcgagcg cgagacggtt gcgccagccc gcggcattct
     3241 tctgcagcca cagcaacgcg cgcaccaggc gctgctcgtc tgcgtcgaag tcggtgccga
     3301 gcggaaacat cggcagcaag ccgcgccccg caagcggcgc aagccgcgca gcgagccgct
     3361 cctcggtatt accgagcgca cgttcgggga cgcgccagtc gcgcggcagc ttgccggcgg
     3421 ccttggcgcg cgcggccagc ccgccctgga agcggcggtc ggcgatcgcg atcagcgccg
     3481 ccgcgacttc ggcgtcggtg cgaccgcgca gatcggcgat gccatattgc gtgacgacca
     3541 cgtcgcgcag gtggcgcggg atcgtctcgt ggccgtagtt gaacacgatg ttcgattcca
     3601 cgctgccgcc cgattcacgg gtcgcgcgca gcagcaggat cgagcgggcg ccctcgagct
     3661 cgtgcgccat cgcgacgaag ttgtactggc cgccgacgcc gctcaccacc ctgccgtccg
     3721 cgaggccgtc cgagaccgcc gcgccgaaca gcgtcacctt catgcagatg ttgatgaaac
     3781 gcgcatgcaa tctctgccgc ctgccgagcg cttcctcgcc gtacaggtcg ttgatgcgcg
     3841 aaaccgaggt catttcgaac agcccgcgtt cctcctcgct cagctcgtgc agtgcccggt
     3901 agaactcgct gggcccgagg aagaacgcgc cttgcagcac cgggccgtcg gcgccgttgg
     3961 cccggcgccg gatcacaccc gcgcggtaga gcgtcatcag gccccaggtg aacatttcgc
     4021 tcgaagcgaa cagtccctcg gcgaaacgcc cgcgcccgcc ctcgcgctcg atgagcgcat
     4081 gctcggcctc cagcgcctgc agcgctgccc cgaacgatcc cggatcggtg tggcgcaggc
     4141 gcagccagtg cgcgaccgcg tcgccgagcg agccgatacc gagctgcaga gtgccgccat
     4201 ccttcaccag cgccgcggca tgcatgccga tcgcatggtc gacgggctcg gcggcggggt
     4261 ttggcacgcc gaagagccgg tgctcccagc gcggcgcgtc caccaggaca tcgaagtagt
     4321 ccccgcctac ctcggcatcg tgcagcatga aaggcaggtt gcggttgacc aggccggcta
     4381 cgacgcaagg ccggccgccc gcgcgcatcg cccgcgccac gtcgagcgtg aggtccgggt
     4441 tgcagctcag gctgtagcgg ccgccgcgct cggcgaccat gacgagcacc gcgttgacgc
     4501 cgcgcgcgcg catgtcgcgc gcgacgtggg tgtagttgct gctgatataa ctttgctgcg
     4561 cggccggcac gccgagcatc gctcccggcc ggaagtagaa ctcgctcagc tcgacgttcg
     4621 cgggcagccg gcccgagcgc aggtcgcgca tgtagtcgag ctcgggcact cccgcgaagg
     4681 cgcggcgggt atagggctcg agaaagcgcc gctcgagctc acccgaggcg cgtggcacct
     4741 cgagggagag cgcgctgatg atcgacaggt gcagccgcgc ctgcgccttg acgcgggcgt
     4801 acagggcgtt gagcaacggc accggctttc ccagcccgag gggcgtcccg cagaccacac
     4861 gtttccccgt gcgcgagacg attgcatcaa ccgcggcgcc caagtccggc gttcgcagga
     4921 ctgtcagagg ctctccgcga gaaggtccag ggctgcggcg gcgaacgcat acatgttgcg
     4981 gacccggtcg ctgctgccgg tcttcagggt gatggcgcgc tcgctgcgtc cggaaacggc
     5041 caggcacgcg tgcccggcgg gatcgccgta gcggttgccg gctgggccgg tggcgcccgt
     5101 ctcgccgatc ccccagtcgg ccgcgaagcg ctcgcgggcg aggcgtgccc ggatcagcgc
     5161 gttgccctcg gtcgccgagc gcttgccctc gagcagatct tcccggaaat cgcgcagcga
     5221 gcgccacgcc tggcgcgtat agatgaccgt gccgcccagg cagtaggccg aagcgcccgg
     5281 gacccccaac aaagcggcgg tgatcagtcc acctgccgag gattcggcga ctgccacggt
     5341 ttccttgcgc tccttcaaca gggccgcgac ccgttcggca tgaggcagga gctcgcgcat
     5401 cggcatattt tgccgaatgg gcaggaatta acacccgaaa gacgcacttc ggggtacgct
     5461 tccaaaagag ggcagtcgaa agggcacgtc gttcatgaga acgaacacca cgctggtgat
     5521 gaatcccaag ggcgggtcgg gtaagaccac cgtcgccacc aatctcgcca gctacttcgc
     5581 cagcaacaat cttgcgacca ccctcatgga ctacgaccct caagggtcca gcctgaactg
     5641 gctgcggctg cgtccgcccc acgcaccgag gatccacggc gccaacggcg cgccgccgaa
     5701 ggccgggcgg ctgcgcagca tcgacatgca cgttccgccg gagacccggc agctgatcat
     5761 cgatgcgccc gcgggcgcct cgggcctgct gctccaagag ctgctctcca gggcccattg
     5821 catcctcatc cccgtggcgc cttccgtaat cgacatccac gccaccgcga acttcatcag
     5881 ggacttgctc atcgcgggca agatccgggc caacaacatc aggctggcag tcgtggcgag
     5941 ccgggtgcgc aggtcgatgc cggtctacca gccgctcgag cgattcctca attccctcaa
     6001 cctgccgctg ctcgcgcgcc tcggcgattc ggacgtctac gtcggcgccg ccgagtccgg
     6061 gatcggcatt ttcgagatgg atttcgcggc ctcgtgcgcc gagcgccagc aattcctgcc
     6121 gatcgccgaa tgggtggaca gcctgcagct gatcgtggcc agcgtgcaga ccccgctgcc
     6181 gtcccgccct gccccgattc ttgcgccagc gcggcccgga ttgcgcgtga taggcaacgg
     6241 cacttcttct tttccaggca ttcgccggta ataaaaaggc ccgggcgatt taggctcggg
     6301 ccttagtgtc tagaattatc cggatatggc tgtcctgcgt atcgccaaga tgggcaatcc
     6361 ggtgctgtcg caggtcgcgg ctccggttgc agatccaacc gcgcccggaa tcaaaaagct
     6421 cgccgccgac atgcgggaca cgatcgagga catcggcgcg agcggcctgg ccgcgccgca
     6481 ggtcttcgaa aacaaacgcg tggtggtgta ccgcatcatc gcttcgcgca tcccggaagg
     6541 ttccgggctc gagccgcggc catggacggt gatggtcaac ccggtgatca agcccaagac
     6601 ggaccagaag acgctggtgt gggagcgctg cctgtcggtt cccggcctgc acggcaaggt
     6661 gccgcgtttt tcgcacatca cgatcagcta ccaggacctc gacggccgca gcgcgagcca
     6721 tgacgcgagc agttcgtggg cggcgctgct gcagcatgaa tgcgaccacc tcgacggcat
     6781 cgtctatccg atgcgcatga ccgatctgcg gctcctcgcc tacaacgacg agcccggtcc
     6841 gctcgcgcgc gaggtcgctg ccgacccgaa gggcatcgac cccttgttca tcgacctggt
     6901 cgagcgctgg ccggggcgcg agcgctggat tggctaaact atcgatactc acgaagggaa
     6961 ccggccatga ctgaaaatca gggcgtcacg cagatggaaa tcgaccagcg cgtcttcgaa
     7021 ctgtatgacg agtactgcca cggccgcatc gaccggcgcg aattcctgaa acgggcgagc
     7081 gcgctcagcg tcgccgggct ggcaatggcg caggcgctgc tgccgcgcta cgcgcaggcg
     7141 cagacgatct ccttcaccga cgcgcgaatc aaggcgcagt acgtgattta cccgtctccg
     7201 ggcggcaa
//
LOCUS       DASPXW010000049         8101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_519008, whole genome shotgun sequence.
ACCESSION   DASPXW010000049 DASPXW010000000
VERSION     DASPXW010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8101
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519008"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VEL04_01135"
     CDS             <1..380
                     /locus_tag="VEL04_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT46998.1"
                     /translation="HFDRDKARELRDDNWFNYGLGARYRLPGEGLDWFFDAGAYHDSG
                     RNTAVLAGAGGFWHATANLRLGAALAFLHSKTYNDGQAFVAPLPVAAYEWRSATLNMV
                     YLPKVHEINSINTLGFWLTVWLR"
     gene            394..1185
                     /locus_tag="VEL04_01140"
     CDS             394..1185
                     /locus_tag="VEL04_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HYT46999.1"
                     /translation="MSTVPNHALRQLRAGKLAVGLGLRQARTVDIAQIARTAGFDWLF
                     IDCEHGSMDADTAAQISAASLAVGITPIVRVPGLEHWHASRLLDNGAQGIVVPHVDTA
                     AEAKRVADTCRYPPLGKRSMGGGLQQLGFEPVPVGEAARIINEETLVVVMIESPQGLE
                     NCEAIAAVKGIDALLIGTNDLCLELGIPGKFDDPKVAEAYRKVIAACRKHGKFPGMGG
                     MYTPELLERHIAMGVQLVLSGSDYSLLMQGAAARAKLVRGFEGKA"
     gene            1209..1445
                     /locus_tag="VEL04_01145"
     CDS             1209..1445
                     /locus_tag="VEL04_01145"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HYT47000.1"
                     /translation="MGKAVKKTISLPADLAREAEARAKAEGKTLSAVIEEALRQTRIE
                     RRAQELGRIQGFWSRKARERGILTEQDLERYLAK"
     gene            1442..2326
                     /locus_tag="VEL04_01150"
     CDS             1442..2326
                     /locus_tag="VEL04_01150"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYT47001.1"
                     /translation="MKNPRVFLDYDQKALDDAYDQSAYAPNREQLLARFARASRIIRE
                     RLGEPQRVSYGPGEIEKLDVYKTRKPDAPVNVFIHGGAWRAGLARDYAFPAEMLVRAG
                     AHFVVVDFNNVLETKGDLLPMADQVRRAVAWVFGNCSLFGGNAARLYVSGTSSGAHLG
                     GCLAATDWQKDFGLPADMVKGYVLASGMYDLRAPRLSRRSSYVKFTDAMEAALSPQRH
                     IGNIKAPLVLLYGSLETPEFQRQSRDFAAALGQAGKPFELIVAEGYNHFELTETLGNP
                     YGAVGRAVLAQMALAPTP"
     gene            complement(2362..2880)
                     /locus_tag="VEL04_01155"
     CDS             complement(2362..2880)
                     /locus_tag="VEL04_01155"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HYT47002.1"
                     /translation="MTALVFVDADVLVYARDGRDARKQARAAQWLTGLWRERTGRTSM
                     QVLSEYYATVTRKLAPAVPAHKAWDDVRELLSWQPHPVDAELLVRAREAEARWRLSWW
                     DSLIVAAAQLQNCTVLLTEDLQDGMAFGTVRVRSPFTLAAGEPEADYAVQDVPVLLHR
                     PRGRPRRVSARA"
     gene            complement(2852..3103)
                     /locus_tag="VEL04_01160"
     CDS             complement(2852..3103)
                     /locus_tag="VEL04_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HYT47003.1"
                     /translation="MKNVTITLDEETAARARVHAAERGMSLSRYVGEALREHLRSSDE
                     YEHAMKRWLARGPFKELTGPPQRYPKREELYDRPGLRRR"
     gene            complement(3152..3520)
                     /locus_tag="VEL04_01165"
     CDS             complement(3152..3520)
                     /locus_tag="VEL04_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47004.1"
                     /translation="MNKIACFAGALALTAGTAFAQAPAEKGEVRRDTRDIRKDRRDIR
                     EDRRDIAKDKADIRKDRRKLREERREGDKAGAAATRADIAKDRQDLRRDEKDLRRDKR
                     DLRKDKRERREDVRENRKGG"
     gene            complement(3678..4175)
                     /locus_tag="VEL04_01170"
     CDS             complement(3678..4175)
                     /locus_tag="VEL04_01170"
                     /inference="COORDINATES: protein motif:HMM:NF019416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HYT47005.1"
                     /translation="MAINRALTPNSGAAFSRCRRWRYLLWRRWDARGPVANFLMLNPS
                     TADEVSLDPSCTRARSYAERWGFGAVLITNLFAWRATDPEEMKRSRDPVGRGNDAAIL
                     RASREAGVVVCAWGNHGAHLGRAAKVVADLRRAGVRLHFLKINGTGEPAHPLYLPGAL
                     EPVRW"
     gene            complement(4209..4373)
                     /locus_tag="VEL04_01175"
     CDS             complement(4209..4373)
                     /locus_tag="VEL04_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47006.1"
                     /translation="MKSSSRIGPIVYEAAQETLALLKILARGNRQIEQGRTIPAAEAF
                     RRLRGKKPRR"
     gene            4443..5402
                     /locus_tag="VEL04_01180"
     CDS             4443..5402
                     /locus_tag="VEL04_01180"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYT47007.1"
                     /translation="MQRFSKAAAALALLLCAGSALPQYPAKPIRFIVGFPPGGSADPT
                     TRIIGQALSEQMGQPFVVENRPGGDSAIAAEAVIRVAADGYTIFFASNSAMTAAVALR
                     KQPAYDPLKDFTPISMVGRATVIFYSHPSVPGTTLGEFIAHAKANPGKLNYGTGNPLS
                     ILNNVQLMSSTGISMVHVPYKGEGPLMPDLLAGRVHSSFLSTGAAIPHAKDGRLRALA
                     VLLDRRSPLLREVPTFDEAGVPQVTVRQWAGVFGPPKMPREIVARLNREVNAAVRRPE
                     VIEKLQSYGYAAEGSTPERLLEINRDDLALWRKLVNEAGIPLE"
     gene            5399..6787
                     /locus_tag="VEL04_01185"
     CDS             5399..6787
                     /locus_tag="VEL04_01185"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYT47008.1"
                     /translation="MKPPDSHPLLVPAIAASQFAPPFMISGVAVALPALGTDLAAGAT
                     ALSLVETLFLAAAVALLLPAGRLGDAADKATLYKLGMAAFALSSILAGLASSMPAILA
                     LRFVQGVTSAAVQATGPAIIADAVPPERRGRAYGITIGSIYAGLTLGPICAGFLVDLW
                     GWRAVFLAGGALVLVLLAPIHFMLRAAWRRPPAGAVHWPSTVLVTAAMLALVGGAATV
                     REGAVGYGAMLLGLFLFFPFVYWQRRLAQPLLNVELLLRNHVLWKALLVQWLLYCNAF
                     GTVFLLSLYMQTVLAHGANTAGQVLAVGTLLMAAIAPFAGSLADRIRPAVIASCGVAV
                     VLIAALMATALGAGSHLVFVGLVLAVQGVGFAFFSSPNMTMVMNAVPPNRTSIASALS
                     ATARSLGMVSGMLIVAALVSLNLGHDPVGAHPARYVATMHTSFWILAAVTFAALAASL
                     LNKKRSHPEVAS"
     gene            complement(6990..7259)
                     /locus_tag="VEL04_01190"
     CDS             complement(6990..7259)
                     /locus_tag="VEL04_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HYT47009.1"
                     /translation="MKLQMHRNSLFAVLLRSPWWGSALVACKRWKAVRTGVEPLREFH
                     AAAAQREAHERIYIAAGEVTDNARAFAAEKSIRLLQGEELAALLH"
     gene            complement(7306..7767)
                     /locus_tag="VEL04_01195"
     CDS             complement(7306..7767)
                     /locus_tag="VEL04_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HYT47010.1"
                     /translation="MTLDNLARIGKLKAHAADEREIGRLLESAARALKDASVATLSSD
                     SRLELAYRALMQAALAAILANGYRPSTSEPGHHQLLIQMLPKTIGIAPGRVQVLEAYR
                     KARNQSDYRGVPVSDAVARDCAEEARLLLADVRAWIEARHTKPPKPPRPAS"
     gene            complement(7712..>8101)
                     /locus_tag="VEL04_01200"
     CDS             complement(7712..>8101)
                     /locus_tag="VEL04_01200"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HYT47011.1"
                     /translation="DLRNILKKTVGVADVLREALAPLAARIRAAFVYGSIARGEERAG
                     SDLDLMVVGEVRFAEVVSALAPAQELLRREVNPSVYPALEFRRKRAAGEPFLKRVLAD
                     RKIFIIGGEHDLGQPGAHRKAEGARRR"
BASE COUNT         1195 a         2936 c         2676 g         1294 t
ORIGIN      
        1 accacttcga ccgcgacaag gcgcgcgagc tgcgcgacga caactggttc aactacggcc
       61 tgggcgcgcg ctaccggctg ccgggcgagg gcctcgactg gttcttcgac gccggcgcct
      121 accacgactc ggggcgcaac accgcggtgc tcgccggcgc gggcggcttc tggcacgcca
      181 ccgcgaacct gcgcctcggc gcggcgctcg cctttctgca cagcaagacc tataacgacg
      241 gccaggcctt cgtcgcgccg cttccggtcg cggcctacga atggcgctcg gcgacgctca
      301 acatggtgta cctgccgaag gtgcacgaga tcaacagcat caacacgctc ggcttctggc
      361 tgaccgtttg gctgcgctaa ggagaaaact tccatgagca ccgtcccgaa ccacgccctg
      421 cggcagctgc gcgccggcaa gctcgccgtc ggcctggggc tgcgccaggc gcgcaccgtc
      481 gacatcgcgc agatcgcgcg caccgcgggc ttcgactggc tgttcatcga ctgcgagcac
      541 ggctcgatgg acgccgacac cgcggcgcag atctccgccg cctcgctggc ggtgggcatc
      601 acgccgatcg tgcgcgtgcc gggcctcgag cactggcacg cctcgcgcct gctcgacaac
      661 ggcgcgcagg gcatcgtggt gccgcatgtc gatacggccg ccgaggcgaa gcgcgtcgcc
      721 gacacctgcc gctacccgcc gctcggcaag cgctcgatgg gcggcgggct gcagcagctc
      781 ggcttcgagc ccgtccccgt gggcgaggcg gcgcgcatca tcaacgagga gacgctggtg
      841 gtggtgatga tcgagtcgcc gcaggggctg gagaactgcg aggcgatcgc cgcggtgaaa
      901 ggcatcgacg cgctgctcat cggcaccaac gacctgtgcc tcgagctcgg catcccgggg
      961 aagttcgacg acccgaaggt cgccgaggcc taccgcaagg tgatcgccgc ctgccgcaag
     1021 cacggcaagt tccccggcat gggcggcatg tacacccccg agctcctcga gcgccacatc
     1081 gccatgggcg tgcagctcgt cctcagcggc tcggactaca gcctgctcat gcaaggcgcc
     1141 gccgcgcggg cgaagctggt gcggggtttt gaaggcaagg cttaggcggg cggtggcatg
     1201 atctagtcat gggcaaagca gtgaagaaga ccatctcgct tcccgccgac ctcgcgcggg
     1261 aagccgaggc gcgcgcgaag gctgaaggca agactctcag cgcggtcatc gaggaagcct
     1321 tacgccagac gcgcatcgag cgccgggcgc aggagctcgg ccggatccag ggcttctgga
     1381 gccgcaaggc gcgcgagcgc ggcatcctca ccgagcagga tctcgaacgc tacctcgcca
     1441 agtgaaaaac ccgcgcgtat ttctcgacta cgatcagaag gcgctcgacg acgcctacga
     1501 ccagtcggcg tacgcgccga accgcgagca gctcctcgcg cgatttgcgc gcgcgagccg
     1561 gatcatccgg gaacggctgg gcgaaccgca gcgggtttca tatggtcccg gagaaatcga
     1621 aaagctcgac gtttacaaga ctcgaaaacc ggatgccccg gtcaacgtct tcatccacgg
     1681 cggcgcctgg cgcgccgggc tcgcgcgcga ctacgccttc cccgcagaga tgctggtgcg
     1741 cgcaggcgcg catttcgtcg tcgtcgactt caacaacgtg ctcgaaacga aaggcgatct
     1801 cctgccgatg gccgaccagg tgcgccgcgc agtggcgtgg gtcttcggga attgctctct
     1861 cttcggagga aacgcagccc ggctttacgt ctccggcacc tcatcaggcg cgcacctggg
     1921 cggctgcctc gccgccaccg actggcagaa ggacttcggc ctgcccgcgg acatggtgaa
     1981 gggctacgtg ctcgcgagcg gcatgtacga cctgcgcgcc ccgcggcttt ccaggcgctc
     2041 gagctacgtg aagttcaccg acgcgatgga agccgcgctc tcgccgcagc ggcatatagg
     2101 aaacatcaaa gcgcccttgg tgctgctcta cggctcgctc gagacgcccg agttccagcg
     2161 ccagtcgcgc gacttcgccg ccgcgctcgg gcaggcgggg aagcccttcg agctgatcgt
     2221 cgccgagggc tacaaccact tcgagctcac ggagacgctc ggcaatccct acggcgccgt
     2281 cggccgcgcg gtgctggcgc agatggctct ggccccgacc ccctgataag ggggcgcgag
     2341 cttttgcgag cgggggtttc gctaggctcg cgcgctgacg cgcctcggcc gcccgcgcgg
     2401 gcgatgcagc aataccggta catcctgcac cgcgtagtcg gcttcgggct cgcccgcggc
     2461 gagcgtgaac gggctgcgca ccctgaccgt gccgaacgcc atgccgtcct ggaggtcctc
     2521 cgtgagcagc accgtgcagt tctggagctg cgcagccgcc acgatcaggc tgtcccacca
     2581 ggagagacgc cagcgcgcct cggcttcccg cgcacgcacc aaaagctcgg cgtccacagg
     2641 atggggctgc catgagagga gttcgcgcac atcgtcccac gccttgtggg ccggcacggc
     2701 cggagccagc ttgcgcgtga cggtggcgta atactccgaa agcacctgca tgctcgtgcg
     2761 tcccgtgcgc tcgcgccaca gcccggtcag ccattgcgcc gcccgcgcct gcttgcgcgc
     2821 atcgcgtcca tcgcgcgcgt aaacgaggac gtcagcgtcg acgaagacca gggcggtcat
     2881 agagctcttc ccgcttggga tagcgctgcg gcggcccggt gagttccttg aaagggcctc
     2941 tcgcgagcca gcgcttcatc gcgtgctcgt attcgtcgga gctccgcaga tgctcgcgca
     3001 gcgcctcgcc gacgtagcgc gacaggctca tgccgcgctc cgccgcatgc acgcgcgctc
     3061 gcgccgcagt ctcctcgtcg agcgtgatgg tcacattctt cacggtccac cttaattaca
     3121 cggagaccgt gtattttaaa gcaacccggg gttacccgcc cttgcggttt tcccggacgt
     3181 cctcgcgacg ctcgcgcttg tccttgcgca ggtcgcgctt gtcgcggcgc aggtccttct
     3241 cgtcgcggcg caggtcctgc ctgtctttcg cgatgtccgc gcgcgtcgcg gccgcgcccg
     3301 ccttgtcgcc ctcgcggcgc tcttcgcgca gcttgcgccg gtccttgcgg atgtccgcct
     3361 tgtccttggc gatgtcgcgg cgatcctcgc ggatgtcgcg ccggtccttg cggatgtcgc
     3421 gggtgtccct gcgcacttcg cccttttcag cgggcgcctg cgcaaaagcg gtaccggcgg
     3481 tcagcgccag cgcgccggcg aagcaagcaa tcttattcat catggccaaa ctcccgaatg
     3541 gttgaatgga acgcccgcgg gtcgccccgc aggctgccca tctaacgcgc cgccggctca
     3601 ggggttgtcc tgggattgtt gcaaggcgta actcggcccc ctgacaaggg ggcagcgagc
     3661 tctttgcgag cgggggttta ccagcgcacc ggctcgagcg ctccgggcag ataaagcgga
     3721 tgcgccggct cgcccgtgcc gttgattttc aggaaatgca gcctcactcc ggcgcggcgc
     3781 aggtccgcca ccaccttcgc ggcgcgcccg agatgcgcgc catggttgcc ccatgcgcac
     3841 accaccacgc ccgcctcgcg cgacgcacgc aggatcgccg cgtcgttgcc gcgccctacc
     3901 ggatcgcgcg agcgcttcat ctcctccgga tcggtcgcgc gccaggcgaa caggttggtg
     3961 atcagcaccg ctccaaaacc ccagcgctcg gcgtagctcc gcgcccgcgt gcacgacgga
     4021 tcgaggctca cctcgtccgc ggtgctcggg ttgagcatca ggaagttcgc gacgggcccg
     4081 cgcgcgtccc agcgccgcca caggaggtag cgccagcgcc ggcagcgcga gaacgccgcg
     4141 ccggaattcg gggtcagagc ccgattgatt gccacatcac ccggtcagca gccggcgcgc
     4201 ggccagtatc agcgccgcgg tttcttgccg cgcagcctgc gaaacgcctc tgccgccgga
     4261 atcgtcctgc cctgctcgat ctgccggttg ccgcgcgcga ggatcttcag caacgcgagc
     4321 gtctcctgcg cggcctcgta gacgatcggc ccgatcctgg aagaggattt catcgcaccg
     4381 aatttagtcc tgtttcggtc ctggcgctag aacgactaca gttacgactc gaggaggtcc
     4441 ccatgcagag attctcgaag gccgcggctg cgctcgctct gctgctctgc gcgggctccg
     4501 cactacctca gtaccccgcg aagccgatcc gcttcatcgt cggcttcccg cccggcggct
     4561 cggccgatcc cacgacgcgc atcatcggcc aggcgctctc cgagcagatg gggcagcctt
     4621 tcgtggtcga gaaccggccc ggcggcgaca gcgcgatcgc cgccgaggca gtgatccgcg
     4681 tggccgccga cggctacacc atcttcttcg cctccaacag cgcgatgacc gcggcagtgg
     4741 cgctgcgcaa gcagccggct tacgacccgc tcaaggactt cacgccgatc tcgatggtcg
     4801 ggcgcgcgac ggtgatcttc tattcgcacc cgagcgtgcc ggggaccacg ctcggcgagt
     4861 tcatcgcgca cgcgaaggcc aatcccggca agctgaacta cggcaccggc aatccgctct
     4921 cgatcctgaa caacgtgcag ctcatgagct ccaccggcat cagcatggtg cacgtgccct
     4981 acaagggcga ggggccgctg atgccggacc tgctcgccgg ccgcgtgcac tcgagcttcc
     5041 tcagcaccgg cgccgcgatc ccgcacgcca aggatgggcg cctgcgcgcc ctggcggtgc
     5101 ttctcgaccg caggagcccg ctcctgcggg aggtgccgac cttcgacgag gccggcgtgc
     5161 cgcaggttac cgtgcgccaa tgggccggcg tgttcggccc gccgaagatg ccgcgcgaga
     5221 tcgtcgcgcg gctcaacagg gaagtgaacg ccgccgtcag gcgccccgaa gtgatcgaga
     5281 agctgcagag ctacggctac gccgccgagg gctcgacgcc cgagcgcctg ctggagatca
     5341 accgcgacga cctcgcgctg tggcgcaagc tggtgaacga agcgggaatc ccgctcgagt
     5401 gaagcccccg gacagtcatc cgctgctggt gccggcgatc gccgcgtcgc agttcgcgcc
     5461 gccgttcatg atctcgggcg tcgcggtggc gctgccggcc ctgggcacgg acctcgccgc
     5521 cggcgccacg gcgctctcgc tggtcgagac gctgttcctc gccgcggccg tcgcgctgct
     5581 gctgcccgcc gggcgcctgg gcgacgccgc cgacaaggcg acgctctaca agctcggcat
     5641 ggcggcgttc gcgctgtcgt cgatcctcgc cgggctggcg tcctcgatgc ccgcgatcct
     5701 cgccctgcgc ttcgtgcagg gcgtcacctc ggcggcggtg caggccaccg gccccgcgat
     5761 catcgccgac gccgtgccgc ccgagcgccg cggccgcgcc tacggcatca ccatcggctc
     5821 gatctacgcc ggcctcaccc tcgggccgat ctgcgccggc ttcctcgtcg acctgtgggg
     5881 ctggcgcgcc gtgttcctcg ccggcggcgc gctggtgctc gtcctgctcg cgccgatcca
     5941 cttcatgctg cgcgcggcgt ggcggcggcc gccagccggc gcggtgcact ggccgagcac
     6001 agttctcgtc accgcggcga tgctcgccct ggtcggcggc gcggcgaccg tgcgcgaggg
     6061 cgcggtgggc tatggcgcca tgctgcttgg cctgtttcta ttcttcccgt tcgtctactg
     6121 gcagcgccgc ctcgcgcagc cgctcctcaa cgtcgagctc ctgctgcgca accacgtgct
     6181 ctggaaagcg ctcctcgtgc agtggctgct ctactgcaac gccttcggca cggtgttcct
     6241 gctctcgctc tacatgcaga ccgtgctcgc ccacggcgcg aacaccgcgg gccaggtgct
     6301 cgccgtcggc acgctcctca tggccgcgat cgcgcccttc gccggcagcc tcgccgaccg
     6361 catccgcccg gccgtcatcg cgagctgcgg cgtcgctgtc gttctcatcg ccgcgctgat
     6421 ggcaactgcg ctcggcgccg gctcgcacct cgtcttcgtc ggcctggtgc tcgcggtgca
     6481 gggcgtcggc ttcgccttct tctcctcgcc caacatgacg atggtgatga acgccgtgcc
     6541 gcccaaccgc accagcatcg cctcggcgct cagcgccacc gcgcgctcgc tcggcatggt
     6601 gagcggcatg ctgatcgtcg ccgcgctggt ctcgctcaac ctcggccacg accccgtcgg
     6661 cgcccacccg gcgcgctacg tcgcgaccat gcacacctca ttctggatcc tcgccgccgt
     6721 gaccttcgcc gcgctggcgg cgagcctgct gaacaaaaaa agaagccacc cggaggtggc
     6781 ctcttagggg tgagccgacc gcctagggag gggaaggcag aggctcaggg ggagcccatt
     6841 gtggaaagcc gccgcggccc tgtctgtcgc acgcgcctgc tttgcgtgta gacggagcgc
     6901 tacaggagaa ccggaacaaa tcgaggattt tgtggccccc tgacaagggg gccgtgagcc
     6961 gcggcaacgc cgcggcgagc ggggggttct cagtgcagga gcgccgccag ctcctcgccc
     7021 tgcagcagcc ggatgctctt ctcggcggcg aaggcgcgcg cgttatccgt gacctccccg
     7081 gcggcgatgt agatgcgctc gtgcgcctcg cgctgcgcgg ccgcggcgtg gaactcgcgc
     7141 agcggctcga cgccggtgcg caccgccttc cagcgcttgc aggcgacgag cgcgctcccc
     7201 caccacggcg aacgcagcag gacggcgaac agcgaattgc ggtgcatctg cagcttcatg
     7261 ccgcgtcgat tttagcggtc tcagaaaatc ggggtcagag cccgattagc tcgccggccg
     7321 cggcggcttc ggcggtttcg tgtgccgggc ttcgatccag gcacgcacgt cggcgagcag
     7381 gagccgggcc tcttcggcgc agtcgcgcgc caccgcgtcg gacaccggca ccccgcgata
     7441 gtcgctctgg ttcctggcct tgcggtaagc ctcgagcacc tgcacgcgcc cgggcgcgat
     7501 gccgatcgtt ttcggcagca tctggatcag aagctggtga tggccaggtt cgctagtcga
     7561 cggccgatag ccgttcgcga ggatcgccgc gagcgccgcc tgcatcagcg cccggtaggc
     7621 gagctccagc cgcgagtcgc tgctcagcgt cgcgaccgag gcgtccttca gcgcccgggc
     7681 agccgattcc agcagccgcc cgatttcccg ctcatcggcg gcgtgcgcct tcagctttcc
     7741 gatgcgcgcc aggttgtcca aggtcatgtt cgccgccgat aatgaagatc ttgcggtccg
     7801 cgagcacgcg cttcaggaac ggctcgcccg ccgcgcgctt tctcctgaac tccaacgccg
     7861 gatagacgct cgggttgacc tcgcggcgca ggagctcctg cgccggcgcg agcgccgaga
     7921 cgacctcggc gaagcggact tcgcccacca ccatgaggtc gaggtcgctg cccgcgcgct
     7981 cctcgccgcg tgcgatcgag ccgtagacga acgccgcgcg gatcctggcg gcaagcggcg
     8041 caagcgcctc gcgcagcacg tccgccacgc cgaccgtctt cttcaggatg ttgcgcaggt
     8101 c
//
LOCUS       DASPXW010000050         2466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_394625, whole genome shotgun sequence.
ACCESSION   DASPXW010000050 DASPXW010000000
VERSION     DASPXW010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394625"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1428
                     /locus_tag="VEL04_01205"
     CDS             <1..1428
                     /locus_tag="VEL04_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008453016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HYT47012.1"
                     /translation="LSAEKCTQHVALLPGTDGALALGMMHVLISENLLDRDYIERYTL
                     GFAELEKRALEDYPPARVAAVCGISTEEVVQLAREYGTVKPAAIRLNYGMQRHAGGGV
                     AARTIACLPALVGAWRDAAGGIVLTTADFYGFDHAALERPDLLAGRRPRAINQSKLGD
                     ALLNARPEIEAAIVYNNNPVAVCPDSEKVVAGFSREDLFTVVLDHFQTDTADYADFVL
                     PATTQLEHWDVHKSYGHLYVLANNPAIPPLGESLPNSEVFRRLAARMGFDEPCFRDSD
                     ENICKTALRSKFDWNQLKKEGWQRLAVPERFAPFAAGGFPTPSGKCEFRSAWLEAQGV
                     DPVPFYNPPAELSDAELARRYPLAFLSPPARHFLNSSFANLARFRQFEREPHLDMHPR
                     DAAERRIGDGDRVRVFNDRGSYELRARVNGKPRPGVVVAPSVWWKKYSPDGRNANDVT
                     SQRTTDLGGGATFYDCRVQVERLGS"
     gene            1425..>2466
                     /locus_tag="VEL04_01210"
     CDS             1425..>2466
                     /locus_tag="VEL04_01210"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HYT47013.1"
                     /translation="MTRLARFLLPHRLRIAAALLALLVAAGCVLALGQGLKHVIDAGF
                     GSGDPRLLNQALAAMIGVAVTLATATWFRFYLMMSTGERVVTDLRRAVFDHVLGLDAA
                     FFEAARTGEVISRLTNDTTLLQQVIGYGLSMFVRNALMMTGAAVMLFVTSWKLAAFVL
                     LGVPATLIPILVLGERVRRLSRASQDRVADVSAYVDEAVHEIRTVQAYAHEDADRDGF
                     ARHAEAAYRVGVARIGQKAFLISAVMLIAFSAVGVILWVGGHDVLAGRLTAGELSAFV
                     FYAIVVATGAGTISEVWGELQRAAGATERLMELLETPPRIVAPATPLAAPPRALRGVE
                     FDAVTFAYPARPG"
BASE COUNT          352 a          861 c          844 g          409 t
ORIGIN      
        1 ctgtcggcgg agaagtgcac gcagcacgtt gcgctgctgc cggggaccga tggcgcactt
       61 gcgctcggca tgatgcacgt gctgatctcg gaaaatcttc tcgaccgcga ctacatcgag
      121 cgctacacgc tcggttttgc cgagctcgag aaacgggcgc tcgaggatta tccgccggcg
      181 agagtcgcgg cggtgtgcgg catttccact gaggaagtag tgcagctggc gcgtgaatac
      241 ggcaccgtca aacccgccgc gatccggctc aactacggca tgcagcgcca tgccggcggc
      301 ggcgtcgccg cgcgcaccat cgcctgcctg cctgcgctgg tcggcgcctg gcgcgacgcg
      361 gcgggcggca tcgtgctgac caccgcggac ttctacggct tcgaccacgc cgcgctcgag
      421 cggcccgatc ttctggccgg gcggcgcccg cgcgccatca accagtcgaa gttgggcgac
      481 gctctgctga acgcccgtcc cgagatcgag gcagccatcg tttacaacaa caacccggtc
      541 gcggtatgcc cggattcgga gaaagtcgtc gcaggctttt cgcgcgagga cctgttcacg
      601 gtggtgctgg accacttcca gaccgacacc gccgactacg cggacttcgt gctgcccgcg
      661 accacccagc tcgagcactg ggacgtgcac aagtcctacg gccacctgta cgtgctcgcg
      721 aacaacccgg cgatcccgcc gctgggcgag tcgctgccga attcggaggt gttcaggcgg
      781 ctggcggcgc gcatgggctt tgacgagccg tgcttccgcg attcggacga gaatatctgc
      841 aagaccgcgc tgcgctcgaa gttcgactgg aaccagctca aaaaagaggg ctggcagcgg
      901 cttgccgtgc ccgagcgctt cgcgcccttc gccgccggcg gcttcccgac gccctcgggc
      961 aaatgcgagt tccgcagcgc atggctcgaa gcgcagggcg tggacccggt gcctttctac
     1021 aatccgccgg ccgaactgtc ggatgcggag ctcgcccggc gctacccgct cgcgttcctc
     1081 tcgccgcctg cgcgccactt cctcaactcg agcttcgcca acctggcgcg ttttcggcaa
     1141 ttcgagcgcg agccgcacct ggacatgcat ccgcgcgacg cggccgagcg ccgcatcggc
     1201 gacggcgacc gggtacgcgt gttcaacgac cgcggcagct acgagctgcg cgcgcgcgtc
     1261 aacggcaagc cgcgccccgg cgtggtggtc gctccctcgg tgtggtggaa gaaatactct
     1321 ccggacggcc gcaacgcgaa cgacgtcact tcacagcgca ccaccgatct cggcggcggc
     1381 gccacgttct acgattgccg ggtgcaagtc gagcgtctgg gctcttgacg cgcctcgcgc
     1441 gctttctcct gccgcaccgc ctgcgcatcg ccgcggcgct gctcgcgctg ctggtcgcgg
     1501 caggctgcgt gctcgcgctc ggccaggggc tgaagcatgt gatcgacgcg ggcttcggca
     1561 gcggcgatcc gcggctgctc aatcaggcgc tcgcggcgat gatcggggtc gcagtgacgc
     1621 tcgcgaccgc gacctggttc cgcttttacc tgatgatgag caccggcgag cgcgtggtga
     1681 ccgacctgcg gcgcgcggtg ttcgaccacg tgctcggcct cgatgcggcg ttcttcgagg
     1741 cggcgcgcac cggagaggtg atatcgcgcc tcaccaacga caccacgctg ctgcagcagg
     1801 tgatcggcta cgggctttcg atgttcgtca ggaacgcgct gatgatgacc ggcgccgcgg
     1861 tgatgctgtt cgtcaccagc tggaagctcg cggccttcgt gctgctcggc gtgccggcga
     1921 cgctgatccc gatcctggtc ctcggcgagc gcgtgcggcg cctgtcgcgc gcgagccagg
     1981 accgcgtcgc cgacgtctcg gcctatgtcg acgaggcggt gcacgagatc cgcaccgtgc
     2041 aggcgtatgc gcacgaggac gccgaccgcg acggcttcgc ccgccatgcc gaggccgcct
     2101 accgcgtcgg cgtcgcgcgc atcggccaga aggcgttcct gatctcggcg gtgatgctga
     2161 tcgcgttcag cgcggtgggc gtgatcctgt gggtcggcgg ccatgacgtg ctcgccggcc
     2221 gcctgaccgc gggcgagctc tcggcattcg tgttctacgc catcgtggtg gcgaccgggg
     2281 cggggaccat ctcggaagtc tggggcgagc tgcagcgcgc cgccggcgcc accgagcggc
     2341 tgatggagct gctcgagacg ccgccgcgca tcgttgcgcc tgcgacgccg ctcgctgctc
     2401 cgccgcgcgc gctgcggggg gtggaattcg acgccgtcac tttcgcctat ccagcgcgcc
     2461 ctggca
//
LOCUS       DASPXW010000051         7163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_646825, whole genome shotgun sequence.
ACCESSION   DASPXW010000051 DASPXW010000000
VERSION     DASPXW010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7163
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646825"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VEL04_01215"
     CDS             complement(<1..293)
                     /locus_tag="VEL04_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYT47014.1"
                     /translation="MDLGIRGRTALVCAASKGLGRGCALSLAREGVNLVITARGREAL
                     EATAEDLRMSFKIKVSTVAGDITTPQGREAALKACASPDILVNNAGGPPPGDFR"
     gene            complement(318..1211)
                     /locus_tag="VEL04_01220"
     CDS             complement(318..1211)
                     /locus_tag="VEL04_01220"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYT47015.1"
                     /translation="MAGYRGWGIAFALVGVLAFSFRPILIKLSYAAHPVSPNTLLFLR
                     MALSLPFFLIIAWWLRGQQPRLTARDWGGVAGLGFIGYYAASFLDFIGLQYVGAGVGR
                     LILFLYPTLVLVISSLFLRNNPTLRQVGALVLSYAGIALVVSDQVGATPEGKLFVLGV
                     MLVFASALCYAIYLVAGSELVKRIGSMRFTAYSMVVATLPAIAQFLVLEPRGALELPP
                     SVWTYAIILATVSTVLPLFLQAEALRRIGATEFALVGAVSPVSVAVMSALGLDEPFGL
                     RQAAGAALVICGVLLVSLKRN"
     gene            1227..1949
                     /locus_tag="VEL04_01225"
     CDS             1227..1949
                     /locus_tag="VEL04_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HYT47016.1"
                     /translation="MSGHSRWANIKHRKAAADAKKGKVFTRLIKEITVAAKLGGGDAD
                     ANPRLRLAMDKAREANMAKDSVQNAVKRGAGQLDGVSYEEARYEGYGPGGAAVMVDCL
                     TDNRTRIVAEVRHAFTKNGGNLGSDGSVAYLFKHCGQFVFAPGTSEDRVMEATLDAGA
                     DDIFRNEDDSVELVCAPEDFPAVKAALAKAGLKPELAELSMKPTIDNPLAGDEAARMR
                     RLLEALESLDDVQNVYTTAVID"
     gene            1946..2461
                     /gene="ruvC"
                     /locus_tag="VEL04_01230"
     CDS             1946..2461
                     /gene="ruvC"
                     /locus_tag="VEL04_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010393321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HYT47017.1"
                     /translation="MIILGIDPGLQVTGFGVIETSGTRLRYVTSGCVKSGAGDLAARL
                     KAILQGLNQVIAAHSPAEVAVEKVFVNANPQSTLALGQARGTAICAAVLAGLPVAEYT
                     ALQVKKAVVGNGHAAKQQVQHMVKRLLALPGDPAADAADALACAICHAHGASLGHLKT
                     SNFRLRKGRLV"
     gene            2458..3024
                     /gene="ruvA"
                     /locus_tag="VEL04_01235"
     CDS             2458..3024
                     /gene="ruvA"
                     /locus_tag="VEL04_01235"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HYT47018.1"
                     /translation="MIGRLTGRLAAKHPPQVLVDVGGVAYELDVPMSTFYGLPATGES
                     VTLFTHLVVREDAHTLYGFGSNDERAAFRRLIRISGVGARTALAVLSGLSVADLAQAV
                     ALQEAARLTKIPGIGKKTAERLLLELKGKLGELATAPSGEKASDVLNALVALGYSEKE
                     ALAATRSLPPGVSVAEGIRAALKALAKS"
     gene            3082..3669
                     /locus_tag="VEL04_01240"
     CDS             3082..3669
                     /locus_tag="VEL04_01240"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HYT47019.1"
                     /translation="MKKSKSVLVILGFAATAVCAQAAAQDTGVYLGGSAGGSYAKNGC
                     TGAAAYSASVPCDSSGTTWGVHAGYQAQYLGFEIGYRNLGTISEQNGGVGQHAEVKSR
                     LGEALLVAGLPVERLRVYGKGGIYRAKSTLKSDFLTEGSSTRAGWTLGAGLSYDITRH
                     LVVRLEYQRYNDLGGDAVGFRTDVDVKTLGVSLRF"
     gene            3666..4703
                     /gene="ruvB"
                     /locus_tag="VEL04_01245"
     CDS             3666..4703
                     /gene="ruvB"
                     /locus_tag="VEL04_01245"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HYT47020.1"
                     /translation="MSVETDRLISAKPSSGEEAQIERSLRPGTLAEYVGQDKVRAQLD
                     IFIQAAKGRSEALDHVLLFGPPGLGKTTLAHIIARELGVSLRQTSGPVLERAGDLAAI
                     LTNLEPRDVLFIDEIHRLPPVVEEILYPALEDFQIDIMIGEGPGARSIKLDLPPFTLA
                     GATTRAGMLTNPLRDRFGIVARLEFYNEAELATIVRRSSRLLKIPLHEDGASEIARRA
                     RGTPRVANRLLRRVRDYAEVKGDGTVSKAVAEAALKMLDVDALGLDVMDRKLLLAVIE
                     KFGGGPVGLDNLAHVVGEDPETIEDVLEPYLIQQGHLQRTPRGRIATLAAYRHFGIAA
                     PAASRDLLSDA"
     gene            4700..5098
                     /gene="ybgC"
                     /locus_tag="VEL04_01250"
     CDS             4700..5098
                     /gene="ybgC"
                     /locus_tag="VEL04_01250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HYT47021.1"
                     /translation="MSRRFSIPLRVYYQDTDAGGVVYHAQYLSFMERARSELLNHAGI
                     DLARFTGESRILFLVFRITIQYHQPARLNDQLTVSAEVVKMGRASLVFRQRVERGAEL
                     LTEADVTLALVDRDSMKPARMPAQIEQALR"
     gene            5095..5775
                     /gene="tolQ"
                     /locus_tag="VEL04_01255"
     CDS             5095..5775
                     /gene="tolQ"
                     /locus_tag="VEL04_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010091489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HYT47022.1"
                     /translation="MTFTQDLDIWTLILHASFVVKVVLAILLLVSFMSWMFIFQKWFA
                     IRRANRKTDEFEREFWSGKDMNALYQGAVNSRHTIGSLERIFEAGFREFAKLRGQRGS
                     ETSDMVDGARRAMRATFQREMDFLERHLAFLASTGSVSPYVGLFGTVWGIMHSFRSLA
                     NVQQATLAQVAPGIAEALVATAIGLFAAIPAVVAYNRYAHDIDRLANRFESFMEEFSN
                     ILHRQAVK"
     gene            5779..6207
                     /locus_tag="VEL04_01260"
     CDS             5779..6207
                     /locus_tag="VEL04_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HYT47023.1"
                     /translation="MPALQRRRGPMHEINIVPYVDVMLVLLVIFMVTAPLVTPAIIDL
                     PSVDKASAPRVVPIEVFVKADQELVVRQRDAAGAIVTERTVSRAELASFIKGARAKGG
                     EPAVLIGGDRNARYEAVLSVMDELHRQGIQKVGLQVKTTK"
     gene            6215..6964
                     /locus_tag="VEL04_01265"
     CDS             6215..6964
                     /locus_tag="VEL04_01265"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HYT47024.1"
                     /translation="MTAATAAQAFSFPQRREPAFVRSFVLAFAVHAILIGVMFLGVRL
                     QSSRPESVTVELWEAPAPPPPPAPVVEAPKPPPPQVEPERPVAKPEIVTVEKPKPKAK
                     PKPEPKRDREFEKRMREQVALEQKAVENQRRASDEQRKERELRELIARQQADARAKAL
                     AAWQEKIRAKIRGNIILPRDLAGNPEAVFDVALLPTGEVLTVKQRKSSGHPGYDEAVY
                     RAILKSSPLPKPEIPGLFDRQLELHFRPQDK"
     gene            6966..>7163
                     /locus_tag="VEL04_01270"
     CDS             6966..>7163
                     /locus_tag="VEL04_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47025.1"
                     /translation="MGLAEKGQTEDALYKRWSLIAAALLALWPPLAGAQLSIEITGAG
                     AQRIPVAIVPFAGEAALPPGLS"
BASE COUNT         1257 a         2417 c         2428 g         1061 t
ORIGIN      
        1 cggaaatcgc cgggcggcgg cccgccggcg ttgttgacca ggatgtcggg cgaagcgcag
       61 gccttgagcg ccgcctcgcg gccttgcggc gtggtgatgt cgcccgctac cgttgagact
      121 tttattttaa agctcattct cagatcttcc gcagtggcct ccagcgcttc ccggccgcgc
      181 gccgtaatga cgaggttgac gccttcccga gcgagcgaca acgcgcagcc cctgcccagc
      241 cccttcgaag ctgcgcacac cagcgcggtc ctgccgcgaa ttccgagatc catggcctag
      301 ctcctgcctt tttagggtca attccttttc agcgatacca gcaagactcc gcaaatcacc
      361 agcgccgcgc ccgcggcctg gcgcaggccg aagggctcgt cgagcccgag cgcgctcatc
      421 accgccacgc ttacggggct gacggctccg accagcgcaa actcggtcgc gccgatgcgt
      481 ctcagcgcct ccgcctggag aaagagcggc agcaccgtgc tgaccgtggc gagaatgatg
      541 gcgtaagtcc acacgctcgg cggcagctcg agcgcgccgc gcggttcgag caccaggaac
      601 tgcgcgatgg cgggcagcgt cgcgacgacc atgctgtaag cggtgaagcg catcgagccg
      661 atgcgcttca ccagctcgct tcccgcaacc aggtagatcg cgtagcaaag cgcggacgca
      721 aacaccagca tcaccccgag cacgaacagc tttccctccg gagtggcgcc cacctggtcc
      781 gagacgacca gtgcgatccc tgcgtagctc agcaccagcg cgccaacctg cctgagggtc
      841 gggttgttgc gcaggaaaag cgatgaaatc accagcacga gcgtcgggta gaggaacagg
      901 atcagcctgc cgacgcccgc gccgacgtac tgcagcccga tgaaatcgag gaagctcgcg
      961 gcgtagtaac cgatgaagcc gaggccggcg acacctcccc agtcgcgagc ggtgagccgc
     1021 ggctgctggc cgcgcagcca ccaggcgatg atgagaaaga acggcagcga cagcgccatg
     1081 cgaagaaaca gcagggtgtt gggacttacc gggtgcgccg cataggagag cttgatcagg
     1141 atcgggcgga acgagaacgc cagcacgccg acgagcgcga acgcgatccc ccagccgcgg
     1201 tagcccgcca tgcgtaaaat catactatgt cggggcacag cagatgggcg aacatcaagc
     1261 accgcaaggc ggctgcggac gcgaagaaag gcaaggtgtt cacgcggctg atcaaggaga
     1321 tcacggtcgc ggcgaagctc ggcggcggcg acgcggatgc caacccgcgc ctgcgtctcg
     1381 cgatggacaa ggcgcgcgag gccaacatgg cgaaggacag cgtgcagaac gccgtcaagc
     1441 gtggcgcggg ccagctcgac ggggtgtcct acgaggaggc gcgctacgag ggctatgggc
     1501 ccggcggcgc ggcagtgatg gtcgactgcc tgaccgacaa ccgcacgcgc atcgtggcgg
     1561 aagtgcgcca cgcgttcacc aagaacggcg gcaatctcgg ctcggacgga tccgtggcgt
     1621 atctcttcaa gcattgcggg cagttcgtgt tcgcgcccgg cacgagcgag gaccgggtga
     1681 tggaggccac gctcgacgcc ggcgcggacg atatcttcag gaacgaagat gattcggtcg
     1741 agctggtgtg cgcgccggag gattttccgg cggtgaaggc ggcgctcgcc aaggccgggc
     1801 tcaagccgga gctcgccgag ctcagcatga agccgaccat cgacaatccg ctcgccggcg
     1861 acgaggcggc gcgcatgcgg cgcctgctcg aggccctcga gagcctcgac gacgtgcaga
     1921 acgtctacac cacggcggtg atcgactgat catcctcggc atcgatcccg ggctgcaggt
     1981 caccggattc ggcgtcatcg agacgagcgg cacgcgcctg cgctacgtca cgagcggctg
     2041 cgtgaaatcc ggcgcgggcg atctcgccgc gcgcctgaag gcgatcctgc agggcctgaa
     2101 ccaggtcatc gccgcccatt ccccggccga ggtggcggtc gagaaggtgt tcgtcaacgc
     2161 caacccgcag tcgacgctcg cgctcgggca ggcgcgcggc acggcgatct gcgcggcggt
     2221 gctcgccggg cttccggtcg ccgaatacac tgccctgcag gtgaagaagg cggtggtcgg
     2281 caacggccac gcggcgaaac agcaggtcca gcacatggtg aagcgcctgc tcgcgcttcc
     2341 gggcgatcct gcggcggatg ccgccgacgc gcttgcctgc gccatctgcc acgcgcacgg
     2401 cgcgagcctg ggtcatctca agaccagcaa cttccggctc aggaaaggca ggctcgtatg
     2461 atcggccggc tcaccgggcg cctcgcggcg aagcacccgc cgcaagtgct ggtcgacgtc
     2521 ggcggcgtcg cctacgagct cgacgtgccg atgagcacct tctacgggct gccggcgacc
     2581 ggcgaaagcg tgacgctttt cacccacctc gtggtgcggg aagacgcgca tacgctctac
     2641 ggcttcggca gcaacgacga gcgcgccgcc tttcgccggt tgatccgcat ctcgggcgtc
     2701 ggggcgcgca ccgcgctcgc cgtgctgtcg ggactttcgg tcgccgacct tgcccaggcg
     2761 gtcgcgctgc aggaagcagc gcggctgacc aagatccccg gcatcggcaa gaagaccgcc
     2821 gagcggctgc tgctcgagct gaagggcaag ctcggcgagc tggcgaccgc gccgagcgga
     2881 gaaaaggcct ccgatgtgct gaacgcgctg gttgcgctcg gctacagcga aaaggaggcg
     2941 ctcgccgcga cgcgcagcct gccccccggc gtctcggttg ccgagggcat ccgcgccgcg
     3001 ctgaaggcgc tcgccaagtc ctgagtttgc gctgcgcagg tctggttgct attgtgcgcg
     3061 gtaccacacg ggggcatggt catgaaaaaa agcaaatcgg tactcgtcat cctcggtttc
     3121 gcggcgacgg cggtctgcgc gcaggcggcc gcgcaggata ccggcgtcta tctgggaggc
     3181 tcggctggcg gctcgtatgc gaagaacggc tgcaccggag cggctgcata cagcgcgagc
     3241 gtcccctgcg acagcagcgg tacgacctgg ggcgtgcacg ccggttacca ggcccaatat
     3301 ctcggcttcg agatcggcta tcgcaatctg ggcacgatca gcgagcagaa cggcggcgtc
     3361 ggccagcatg ccgaggtgaa gtcccgctta ggcgaggcgc tgctggtcgc cggcctgccg
     3421 gtggaacggc tccgggtgta cggcaaaggc ggcatttacc gcgccaaatc caccctcaaa
     3481 tccgatttcc tcaccgaagg cagcagcacg agagccggct ggaccctggg cgccggcctg
     3541 agctacgaca tcacccgcca tctggtggtt cgcctcgagt atcagcgcta taacgacttg
     3601 ggcggtgacg ccgtcggctt caggaccgac gtcgacgtca agaccctcgg cgtcagcctc
     3661 aggttttgag cgtagaaacc gaccgtctga tctcggccaa gccctcctcg ggagaggagg
     3721 cgcagatcga gcggtcgctg cgcccaggca cgctcgccga gtatgtcggg caggacaagg
     3781 tgcgtgcgca gctcgacatt ttcatccagg cggccaaggg ccgaagcgaa gcgctcgacc
     3841 acgtgctgct gttcgggccg cccggcctgg gcaagaccac cctcgcccac atcatcgccc
     3901 gggagctggg cgtgagcctg cgccagactt ctggcccggt gctcgagcgc gccggcgatc
     3961 tcgccgcgat cctcaccaat ctcgagccgc gcgacgtgct gttcatcgac gagatccacc
     4021 gcctgccgcc ggtggtggag gagatcctct acccggcgct ggaggacttc cagatcgaca
     4081 tcatgatcgg cgaaggcccc ggggcgcgct cgatcaagct cgacctgccg cccttcaccc
     4141 tcgcgggggc gaccacgcgc gccggcatgc tcaccaaccc gctgcgcgac cgctttggca
     4201 tcgtggcgcg cctcgagttc tacaacgagg ctgagctggc gacgatcgtg cgccgttctt
     4261 cacgtctttt gaaaatccct ttgcatgagg atggggcgag cgagatcgcg cgccgcgcgc
     4321 gcggcacgcc acgcgtcgcc aaccggctgc tgcgccgcgt gcgcgactat gccgaggtga
     4381 agggcgacgg cacggtgtcg aaggccgtgg cggaggccgc gctcaagatg ctcgacgtcg
     4441 acgcgctcgg cctcgacgtc atggatcgca agctgctgct cgcggtgatc gagaagttcg
     4501 gcggcggccc ggtcgggctc gacaacctcg cccatgtcgt cggcgaggac cccgagacca
     4561 tcgaggacgt gctcgagccc tatctcatcc agcagggcca cctgcagcgc acgccgcgcg
     4621 gccgcatcgc cacgctcgcc gcctaccgcc acttcggcat cgcggcgccc gcggcgagcc
     4681 gcgacctgct ctcggacgct tgagccgacg cttcagcatc ccgcttcgcg tctactacca
     4741 ggacacggac gcgggcggcg tcgtctacca cgcgcagtac ctctctttca tggagcgcgc
     4801 tcgcagcgag ctcctcaacc acgccggcat cgacctcgcg cgcttcaccg gcgagagccg
     4861 catcctgttc ctggtgttcc gcataaccat ccaataccat cagcccgcgc ggctgaacga
     4921 ccagctaacg gtgagcgccg aagtggttaa aatgggccgc gcgagcctgg tgttccggca
     4981 gcgcgtggaa cgcggcgccg agcttctgac cgaggccgac gtgacgctcg cgctggtcga
     5041 ccgcgatagc atgaaacccg cgcgcatgcc cgcacaaatc gaacaggcgc tgagatgacg
     5101 ttcacgcagg atctggacat ctggacgctg atcctccacg cgagcttcgt ggtgaaggtg
     5161 gtgctggcga tcctgctgct ggtgtcgttc atgtcctgga tgttcatctt ccagaagtgg
     5221 ttcgcgatcc ggcgcgccaa ccgcaagacc gacgagttcg agcgcgagtt ctggagcggc
     5281 aaggacatga acgcgctcta ccagggggcg gtcaacagcc gccacaccat cggcagcctc
     5341 gagcgcatct tcgaggcggg ctttcgcgag ttcgccaagc tgcgcgggca gcgcggcagc
     5401 gagaccagcg acatggtcga cggcgcgcga cgcgcgatgc gcgccacgtt ccagcgcgag
     5461 atggatttcc tcgagcggca cctggccttc ctcgcctcga ccggctcggt gagcccgtac
     5521 gtgggccttt tcggcacggt ctggggcatc atgcactctt tcaggagcct ggcgaacgtg
     5581 cagcaggcga ccctcgccca ggtcgcgccg ggcatcgccg aggcgctggt ggccactgcg
     5641 atcggcctgt tcgcggcgat ccccgcggtg gtcgcgtaca accgctacgc gcacgatatc
     5701 gaccgcctcg ccaaccgctt cgagagcttc atggaggagt tctccaacat cctgcaccgc
     5761 caggcggtga agtaagcaat gcctgccctc cagcgcaggc gcgggccgat gcacgagatc
     5821 aacatcgtgc cttacgtcga cgtcatgctg gtgctgctgg tgatcttcat ggtcacggcg
     5881 ccgctggtca cgccggcgat catcgacctg ccgagcgtcg acaaggcgag cgcaccgcgg
     5941 gtcgtgccga tcgaggtgtt cgtcaaggcc gaccaggagc tggtggtgcg ccagcgcgac
     6001 gccgccggcg cgatcgtcac ggagcgcacg gtctcgcgcg ccgagcttgc gagcttcatc
     6061 aagggtgcgc gcgcgaaggg cggcgagccc gcggtgctga tcggcggcga caggaacgcg
     6121 cgctacgagg cggtgctgtc ggtgatggac gagctgcacc ggcaggggat ccagaaggtc
     6181 ggcctgcagg tgaagaccac caagtagggg cgcgatgacc gccgcgacgg ccgcgcaggc
     6241 cttcagcttc ccgcagcggc gcgagccggc tttcgtacgc tcgttcgtgc tcgctttcgc
     6301 ggtgcacgcg atcctcatcg gtgtcatgtt tctcggcgtg cggctgcaga gcagccggcc
     6361 cgaaagcgtg accgtagagc tttgggaggc gccggcgccc ccgccgccgc ccgcgccggt
     6421 ggtcgaggcg ccgaagccgc cgccgcccca ggtcgagccc gaacggccgg tggcgaaacc
     6481 cgagattgtc acggtcgaaa aacccaagcc gaaggctaag cccaagcccg agccgaagcg
     6541 cgaccgggaa ttcgagaagc ggatgcgcga gcaggtggcg ctggagcaga aggcggtcga
     6601 gaaccagcgg cgcgcctccg acgagcagcg caaggaacgc gagctgcgcg agctgatcgc
     6661 ccgccagcag gcggacgcac gcgcgaaagc gctcgccgcc tggcaggaga agatccgcgc
     6721 caagatccgc ggcaacatca tcctgccgcg cgatctggcc ggaaacccgg aggcggtatt
     6781 cgacgtcgcg ctgctgccca cgggcgaggt gctgacggtg aagcagcgca aatcgagcgg
     6841 ccaccccggc tacgatgagg cggtgtaccg cgcgatcctg aagtcctcgc cgctgcccaa
     6901 gcccgaaatc ccggggctgt tcgatcgcca gctggaactt cacttcaggc cgcaggacaa
     6961 ataacatggg tctggcagaa aaaggacaaa cggaggatgc cctgtacaag cgctggtctc
     7021 tgattgccgc ggcgcttctc gcgctgtggc cgccgcttgc cggggcgcag ctttcgatcg
     7081 agatcaccgg cgccggcgcg cagcgcattc cggtcgcgat cgtgcccttc gccggcgagg
     7141 cggcgctccc cccaggcctg agc
//
LOCUS       DASPXW010000052         2447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1530589, whole genome shotgun sequence.
ACCESSION   DASPXW010000052 DASPXW010000000
VERSION     DASPXW010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1530589"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(281..2227)
                     /locus_tag="VEL04_01275"
     CDS             complement(281..2227)
                     /locus_tag="VEL04_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYT47026.1"
                     /translation="MIRLSQVTLRRGTKTLLEGADVALNPGDKIGLIGANGSGKSSLF
                     ALLRGELHADKGEVDYPARWRVAYVAQETPALERTALDYAIDGDTTLRRLERELAAAE
                     AVDDGHLIGELHAAMGDADAYTVRSRAEQLLHGLGFTHEQMGEPVASFSGGWRMRLNL
                     AQALMCPSDLLLLDEPTNHLDLDAIIWLEEWLKRYAGTLLIVSHDRDFLDGVVEVVVH
                     IDAKKLRRYSGNYSSFERQRAAAVVLAASQYENQQRERAHLESFINRFRAKATKAKQA
                     QSRMKMLAKMEELAPLHVSAPFSFEFREPRKAPDPLLVLEDADAGYKTEGEPDKVVVR
                     GINFSLQSGQRYGLLGINGAGKSTLIKTIAEELAPLAGKAIFNKGLAVGYFAQHQIEM
                     LRHDESPLWHLSRIATNVREQELRNYLGSFNFPGDTATTSIATFSGGEKALLALALIV
                     WQRPNLLLLDEPTNHLDLETREALTVALAQFEGTLVLVSHDRHLLRATTDQFLIIAEG
                     RLAPFDGDLEDYRDWLFKTKLAPTEEAVPLPEAKKPAAAAPPAAPARREQKRREAEAR
                     QRQSAARKPIESRIKRLEEKMAKLNAQKAAIDAQLADPASYGEARKDALKTLILDRAY
                     VARELAQLEAEWLEQQQRLGQLAA"
BASE COUNT          370 a          802 c          828 g          447 t
ORIGIN      
        1 ttcctgctcc gcggcaagcg cgtcacccac aaaggccctg cgcttctccg cgagcgcggt
       61 atgcgcttcc agcgcctcga tcatccacgc gtgcggcgtc ttgcccgcgg atttcgcgag
      121 cggcgcgatg cgctgcttca atcgctcgga aagcttgagc gtggtggacg cggccatgaa
      181 aattctctcg agtaacacga aggtaacacc tgcgcgcgag gcgagtcaag cgcggccgcg
      241 atgcgccgtg gatatttgtg gaagagctga tcctctcaga ctaggccgcc agctgcccca
      301 gcctctgctg ctgctccagc cattcggctt cgagctgcgc cagctcccgc gcaacgtagg
      361 cccggtcgag gatcagcgtc ttcagggcgt cctttcgcgc ttcgccatag ctcgccgggt
      421 cggcgagctg cgcgtcgatc gcggccttct gcgcgttgag cttcgccatt ttctcttcca
      481 gccgcttgat gcgcgactcg atcggcttcc tcgccgcgga ctggcgctgg cgcgcttccg
      541 cttccctgcg cttctgctcg cggcgggccg gagcggccgg cggcgccgcg gcggcaggtt
      601 tcttcgcctc gggcaaaggc accgcttcct ccgtgggtgc gagcttggtc ttgaacagcc
      661 agtcgcggta gtcctcgagg tcgccgtcga agggcgcgag gcggccctcg gcgatgatga
      721 ggaactggtc ggtggtggcg cgcaagaggt gccggtcgtg cgagacgagc acgagcgtgc
      781 cttcgaactg ggcgagcgcc accgtcagcg cctcgcgcgt ctccagatcg aggtggttgg
      841 tcggctcgtc gagcaggagc aggttggggc gctgccacac gatgagcgcg agggcgagca
      901 gcgccttctc gcccccggag aaggttgcga tcgaggtcgt cgccgtgtcg ccggggaagt
      961 tgaagctgcc gagatagttg cgcagctcct gctcgcgcac gttcgttgca atgcgcgaga
     1021 ggtgccagag cggcgattcg tcgtggcgca gcatctcgat ctggtgctgc gcgaaatagc
     1081 cgaccgcgag gcccttgttg aagatcgcct tgccggcgag cggtgcgagc tcctcggcga
     1141 tggtcttgat gagcgtcgat ttccccgcgc cgttgatgcc gagaaggccg tagcgctggc
     1201 cgctctgcag cgagaagttg atgccgcgca cgacgacctt gtccggctcg ccttccgttt
     1261 tgtagccggc gtccgcgtct tccagcacca gcagcggatc gggcgctttc cgcggttcgc
     1321 ggaactcgaa cgagaagggc gcagagacgt gcaagggggc cagctcctcc atcttcgcca
     1381 gcatcttcat tcggctttgc gcctgcttcg ccttggtggc cttcgccctg aagcggttga
     1441 tgaaggattc caggtgggcg cgctcgcgct gctggttttc gtactgcgac gcggcgagca
     1501 ccaccgcggc ggcgcgctgg cgctcgaagc tcgagtagtt gcccgagtag cgcctgagct
     1561 tcttcgcgtc gatgtgcacg acgacctcca ccacgccgtc caggaagtcg cggtcgtgcg
     1621 agacgatgag aagcgtcccg gcgtagcgct tgagccactc ctcgagccag atgatggcgt
     1681 cgaggtcgag gtggttggtc ggctcatcca gcagcagcag gtccgaggga cacatcagcg
     1741 cctgcgcgag attcagccgc atgcgccagc cgccggagaa gctcgccacg ggctcgccca
     1801 tctgctcgtg cgtgaaaccg aggccgtgca gcagctgctc cgcgcgcgag cggaccgtgt
     1861 acgcatcggc gtcgcccatc gctgcgtgca gctcgccgat caagtggccg tcatcaaccg
     1921 cttcggccgc ggcgagctcg cgttcgagcc ggcgcagcgt cgtatcgccg tcgatcgcgt
     1981 agtcgagcgc cgtgcgttcc agcgcgggcg tttcctgcgc gacgtaggcg acgcgccagc
     2041 gcgccgggta atcgacctcg cccttgtccg cgtgcagctc gccgcgcagg agcgcgaaca
     2101 ggctcgactt ccccgagccg ttcgcgccga tcaggccgat cttgtcgccg ggattgagcg
     2161 cgacatcggc gccttcgagc agggtcttcg tgccgcggcg gagcgtgact tgggagagac
     2221 ggatcatggg atttcaattc gcaaggcgcg cattgtacgc ggaacggcgc gctcaaccgc
     2281 tcggacgatc ctaactcaaa ccccggcgac ggacgggaga caaaccaggt ttcaccgagt
     2341 gccgcggcag gttttcgcgc cggcggttat cctgtgaatg caatatcatc ccgagaacaa
     2401 cccgaaggaa gacctcgatg caacgatccc agccgtttac gctcgcc
//
LOCUS       DASPXW010000053         3189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_143432, whole genome shotgun sequence.
ACCESSION   DASPXW010000053 DASPXW010000000
VERSION     DASPXW010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143432"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VEL04_01280"
     CDS             <1..186
                     /locus_tag="VEL04_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005882785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HYT47027.1"
                     /translation="WRFGIMGYSCRPDNVMLCLSALGSVLNDMGLAVHVGDAEAAAHG
                     AYAQMHVKDAQRKKRAA"
     gene            218..1261
                     /locus_tag="VEL04_01285"
     CDS             218..1261
                     /locus_tag="VEL04_01285"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HYT47028.1"
                     /translation="MDTLTHALSGALLARATASRSAPPRSIPRRVAAGFFACAAPDLD
                     FIFGFAGPVEYLLNHRGITHSLVLLPLWAFIFSWILAKVLREPGGWRALYGVTALALG
                     AHIAGDVITSYGTMVLAPFSDWRAAIGTTFIIDLWFSGVILAGLAGSAVLFRSRIPSA
                     AGCALLVAYVGFQYVQRQHALEFAEQFVMTKGISGARISAQPRPVTPFNWTVFVSDEE
                     AHRFAHVNLLRTEPKPYRPGDGFVAKLDAPYLPLAQAIWVTRSRYGESDQDFIRQAWN
                     SDALDFFRWFAELPAFDGLTQGSTCAWFVDLRFLTPGRDVMPFQFGACRESPGTPWRA
                     YERIGAGGRMPLR"
     gene            complement(1262..2104)
                     /locus_tag="VEL04_01290"
     CDS             complement(1262..2104)
                     /locus_tag="VEL04_01290"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HYT47029.1"
                     /translation="MRFCIAALLFSLLAAPAQAQFNLDKLNINKLVDTVKNVGKATTE
                     ISEPDEIAIGRDMAARLLGAAHLVADARLQRYVNRVGRWLASQTERPDLPWQFGVLDA
                     PQLNAFAVPGGTIFVTRGLVQRMRSESELAGVLAHEIAHVLRKHHLKAIQKSARTGLA
                     GEALTHALKNSRPEARDKLIGFGTEMYTRGLDKSDELEADRLGVVIAARAGYDSYGLP
                     SVLQTLQAMNAQDSALALMFKTHPAPAERLSALAEKMQPTLDAYASQPQVAERFAAEA
                     RAAK"
     gene            complement(2101..2571)
                     /locus_tag="VEL04_01295"
     CDS             complement(2101..2571)
                     /locus_tag="VEL04_01295"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HYT47030.1"
                     /translation="MRFLLLFAFLLPIACAAEPATVIRATELKQGPATDAPTVAQLPV
                     NTAVDALERKGGWTRVKAPGGEGWVKMLSLRYGGAAKAGDSGVSQLFNVARTGSSGTQ
                     VTTGVRGLDAEQIAAARPNAAELKKLQSFAETRDASSAFAEGGRLQPQRVAYPK"
     gene            complement(2568..>3189)
                     /locus_tag="VEL04_01300"
     CDS             complement(2568..>3189)
                     /locus_tag="VEL04_01300"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYT47031.1"
                     /translation="VNPEVVKQLIERGGIEGAGQQREVTLLFSDIRGFTSLSEKRRPE
                     EVVALLNRYFSLQVDVVFRHGGSLDKFIGDCIMALWGAPLDDREHARRAVACALDMAD
                     TLAVFKREFGEGAKDFDVGIGVHSGPAVVGLIGSEKRREYTAIGDTVNLASRIEGLTK
                     DAKRRILVSRDTMERCADGFDFVSCGTFPVKGRAQPVELFEPRRKP"
BASE COUNT          459 a         1088 c         1063 g          579 t
ORIGIN      
        1 tggcgcttcg gcatcatggg ctactcgtgc cgtccggaca acgtgatgct ctgcctctcg
       61 gcgctcggct cggtgctgaa cgacatgggc ctggcggtgc acgtcggcga cgccgaggcc
      121 gccgcgcacg gcgcgtacgc gcagatgcac gtcaaggacg cccagcgcaa gaagcgcgcc
      181 gcgtaaatcg aaatcgggct ctgaccccga ttttctgatg gacacgctga cccacgcgct
      241 ctcgggcgcg ctgctggcgc gcgcgacggc ctcgagaagc gcgccgccgc gttcgattcc
      301 gcgccgcgtc gccgcagggt ttttcgcttg cgcggcgcct gatctcgatt tcatcttcgg
      361 cttcgccggg ccggtcgaat acctgctcaa ccatcgtggc atcacgcatt cgctcgtgct
      421 gctgccgctt tgggcattta ttttttcctg gatactggca aaagtgctgc gcgagcccgg
      481 ggggtggcgc gcgctctacg gcgtcaccgc gctcgcgctc ggcgcgcata tcgcaggcga
      541 cgtcatcacg agctacggca cgatggtgct cgcgcccttc tcggactggc gtgcggcgat
      601 cggcaccacg ttcatcatcg acctgtggtt cagcggcgtc atcctcgccg gcctggcggg
      661 ttctgccgtc ttgtttcgtt ccaggatccc ttcggcggcg ggctgcgcgc tgctcgtcgc
      721 ttacgtaggc ttccagtacg tgcaacgaca gcatgcgctc gaatttgccg aacaatttgt
      781 catgacgaag ggaatttcag gggcgcgcat ctccgcgcag ccgcggccgg tcacgccttt
      841 caactggacc gtgttcgtga gcgacgagga agcgcaccgc ttcgcgcatg tcaatctgct
      901 caggaccgag ccgaagccct accgtcccgg cgacggcttc gtcgcgaagc tcgacgcgcc
      961 ttacctgccg ctcgcgcagg cgatctgggt gacgcgctcg cgttacgggg aaagcgacca
     1021 ggacttcatc aggcaggcat ggaattcgga cgcgctggac tttttccgct ggttcgcgga
     1081 gctgccggct ttcgacggcc tgacgcaggg ctcgacttgc gcgtggttcg tggacctgcg
     1141 cttcctgacg cctgggcgcg acgtcatgcc gttccagttc ggcgcctgcc gggaatcgcc
     1201 cggcactcca tggcgtgcct atgagcgcat cggcgccggc ggcaggatgc cgctgcgcta
     1261 gctactttgc ggctctcgct tcagcggcaa agcgctcggc gacctgcggc tgcgaggcgt
     1321 aggcatcgag cgtgggctgc atcttctcgg caagcgcgct cagccgctcg gccggcgcgg
     1381 ggtgggtctt gaacatcagt gcgagcgccg aatcctgcgc gttcatcgcc tgcagcgtct
     1441 gcagcaccga cggcaggccg taggagtcgt agccggcgcg cgccgcgatc accacgccca
     1501 gcctatcggc ctcgagctcg tccgatttgt cgaggccgcg ggtgtacatc tccgtgccga
     1561 agccgatcag cttgtcgcgc gcctcggggc gcgagttttt cagcgcgtgc gtcagggctt
     1621 cgccggcaag cccggtgcgc gcgcttttct ggatcgcctt caggtggtgc ttgcgcagca
     1681 cgtgcgcgat ctcgtgcgcg aggacgccgg cgagctcgga ctcgctgcgc atgcgctgca
     1741 ccaggccgcg cgtgacgaag atggtgccgc ccggcaccgc gaaggcgttg agctgcggcg
     1801 cgtcgagcac gccgaactgc caaggcagat cgggccgctc ggtctgggaa gcgagccagc
     1861 ggccgacgcg gttcacgtag cgctgcagcc gcgcgtccgc taccaggtgc gcggcgccca
     1921 gcaggcgcgc cgccatgtcg cggccgatgg cgatttcgtc cggctcgctg atctcggtgg
     1981 tggccttgcc cacgttcttc accgtgtcca ccagcttgtt gatgttcagc ttgtcgagat
     2041 tgaactgcgc ctgcgccggc gcggccaaaa gggaaaagag cagggcggcg atgcagaatc
     2101 tcatttcggg tatgccacgc gctggggctg cagcctgccg ccctcggcga atgcgctcga
     2161 ggcgtcccgg gtttccgcga aggactgcag cttcttcagc tcggcggcgt tgggacgcgc
     2221 ggcggcgatc tgctccgcgt ccagcccgcg caccccggtc gtgacctggg tgccgctcga
     2281 gccggtgcgc gcgacattga agagctgcga gacgccgctg tcgccggcct tggcggcacc
     2341 cccgtagcga agcgagagca tcttcaccca gccttcgccg cccggcgcct tgacgcgtgt
     2401 ccagccgccc ttgcgctcga gcgcgtcgac cgcggtgttt accggaagct gcgccacggt
     2461 cggcgcatcc gttgccggac cttgttttag ttctgttgcc cttattaccg tcgccggctc
     2521 ggcggcgcag gcgatcggca gcaaaaaagc gaataacagc aaaaatctca tggcttgcgc
     2581 cttggctcga agagctccac cggctgcgcc cgccctttca ccgggaaggt tccgcatgat
     2641 acgaaatcga agccgtccgc gcacctttcc atggtgtcgc gcgacaccag gatgcggcgc
     2701 ttggcgtcct tggtgaggcc ctcgatgcgg ctcgcgaggt tcaccgtgtc gccgatggca
     2761 gtgtactcgc ggcgcttctc cgacccgatc agcccgacca ccgcgggacc cgagtgcacg
     2821 ccgatgccga cgtcgaagtc cttcgcgccc tcgccgaact cgcgcttgaa gaccgcgagg
     2881 gtatcggcca tgtcgagggc gcaagccact gcgcggcggg cgtgctcgcg gtcatcgagc
     2941 ggcgcgcccc acagcgccat gatgcagtcg ccgatgaact tgtcgagcga gccgccgtgg
     3001 cggaacacga cgtccacctg cagcgagaag tagcggttga gcaacgccac cacttcctcc
     3061 gggcggcgct tctcggagag cgaagtgaag ccccggatgt cggaaaagag cagcgtcacc
     3121 tcgcgctgct ggcccgcgcc ctcgatgccg ccgcgctcga tgagctgctt caccacctcc
     3181 gggttcacg
//
LOCUS       DASPXW010000054         5224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1028115, whole genome shotgun sequence.
ACCESSION   DASPXW010000054 DASPXW010000000
VERSION     DASPXW010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5224
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028115"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="VEL04_01305"
     CDS             <1..971
                     /locus_tag="VEL04_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009977249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYT47032.1"
                     /translation="HPDDRDLTDAARARVHDGELDSVRFEKRYIRKNGTAVWVELAVA
                     LARDPHGHPLYEVALFDDITARKQAEAELKEAHLELKRSNAELEQFAYVASHDLQEPL
                     RMMSSYTQLLLRRYGDKFDQDAREFMHYVVDGAARMKQLIEDLLAYSKVGTRAKDFGD
                     ADVEAALRRAITNLKAAIGETGAAVTWDPLPKVKADDAQLAQLFQNLIGNALKFRSAS
                     VPRVHVSVSQKDSEWEFSVRDNGIGIEPQYFERIFMVFQRLHDKAEYPGTGIGLAICK
                     KVVERHGGRIWVESQPGAGSAFHFTLPKGFTLPKGFKSSEAEGERP"
     gene            968..1420
                     /locus_tag="VEL04_01310"
     CDS             968..1420
                     /locus_tag="VEL04_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004188466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYT47033.1"
                     /translation="MTDGGSPVEILLVEDNPGDERLTREALREGKVYHNLHWVTDGVQ
                     AMEFLRRQGKYASVPRPDIVLLDLNLPKKDGREVLEEIKTDEDLKRIPVVVLTTSKAE
                     EDVLRTYNLHANCYVTKPVDLEKFIVVVKSIDVFWLTVVTLPPDGHGK"
     gene            1420..3123
                     /locus_tag="VEL04_01315"
     CDS             1420..3123
                     /locus_tag="VEL04_01315"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HYT47034.1"
                     /translation="MHNVLLIEDNPGDARLIAEMIAEHPGAPFVLHTADRLSLGLEHL
                     SAGSTGLVLLDLSLPDSFGLDTFAKVYAHSPTVPIIVLTGNDDHMLALSAVKSGAQDY
                     LVKGRLDRELLLRSMQYSIERKRYQVQLEHQANYDALTGLPNRNLLHDRLRQAVYAQR
                     SPRNIAVVFMDLDHFKFVNDSLGHGTGDKLLKSMGERLRAVLREGDTVGRVGGDEFVL
                     ILNDQSNEEVIFRAMQRISAKVSESITIEGKELYVTCSAGISLYPQDGRDVDTLLKNA
                     DAAMYRAKEHGRNNFQFYTSEMNERVNERLSLEAALRRALERREFLLHYQQKIDLRSG
                     AIIGAEALVRWNHPEWALVRPARFIPLAEETGLIVPLGEWVLHEACRQAREWLDQGLR
                     PGVVSVNLSVRQFRQEGLVRTVSRILEETRLDASQLEMELTESMVMQNVESAIATLQG
                     LKSLGVALSVDDFGTGYSSLSYLKQLPFDTLKIDRSFVRNIGAGEDPDEGVIAQAIIS
                     LGHSLRLKVIAEGVETDAQVRFLKRHGCDEVQGFLYGEPVAAEDYAKLLDKAKRKGKR
                     A"
     gene            3120..3845
                     /locus_tag="VEL04_01320"
     CDS             3120..3845
                     /locus_tag="VEL04_01320"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HYT47035.1"
                     /translation="MRFFLFITLGIFSLAGFCDEIKVLSGGAARGFVEPLAASFQAHA
                     VKLEYQTMGRLMKTMASAEAARYDMLIVTTEVAPKLQLADVKPLARVGIGVAVNEKAP
                     LPDISTVDSFKRTLLAAKSIVYINPQTGTSGKYVVEAFRKLGIEGEMRAKTTLVDSGY
                     AVAPVGRGEIELGIHQISEILPVKGVKLVGPLPPELQKYTVYVAAALPGTQKKPAVDA
                     FIAHLTGTEARSRLAQAGYTSPE"
     gene            3842..5035
                     /locus_tag="VEL04_01325"
     CDS             3842..5035
                     /locus_tag="VEL04_01325"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HYT47036.1"
                     /translation="MSDKARPVILAVDDATDLLALMAQALGGEYEVITASDAGTAIEK
                     AFGEPRPDLILLDIDMPDISGFEVCRALKDEAITTAIPVIFLTGRTEAQAQLEGFELG
                     AVDYVTKPINAAVLMARVRMHLALSNQRQALEHMVQERTGQLDRTRAELIKRLSRAME
                     LHESAAVGNRVMRLGHYAKLIAQAAGAKPEIAEMMLKAAPLHDIGKLGVPAEILRKAE
                     KLSVPDWERVKRHPQLGAEIIGEHDDPLLKLARQLALAHHERWDGSGYPQGLKAEAIP
                     WGGRVMAIVDSFESMTSTQFFRDAIPVDQAAAEIEKGAGTRYEPKLVEAFRKALPVMK
                     KVRETYADKLGDLINLDFAPKGAAKPAAAPAAAAPKPPPAPPKPAKPVVSAAQLAKAA
                     AAKRK"
     gene            5032..>5224
                     /locus_tag="VEL04_01330"
     CDS             5032..>5224
                     /locus_tag="VEL04_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47037.1"
                     /translation="MSKLFEKLKGAERERLEKDRSGAEVAQARAGEERRALDLARERQ
                     QAEVELRRIAHARAEAEAKA"
BASE COUNT          960 a         1754 c         1764 g          746 t
ORIGIN      
        1 cccatcccga cgaccgcgac ctcaccgacg ccgcgcgcgc gcgcgtgcac gatggcgagc
       61 tcgactcggt gcgcttcgag aagcgctaca tccgcaagaa cggcaccgcg gtctgggtcg
      121 agctggcggt cgcgctggcg cgcgacccgc acggccatcc gctgtacgag gtcgcgctgt
      181 tcgacgacat caccgcgagg aagcaggccg aagccgagct caaggaggct cacctggagc
      241 tgaagcgctc caacgccgag ctcgagcaat tcgcctacgt cgcctcgcac gacctgcagg
      301 agccgctgcg catgatgtcg agctacaccc agctgctgtt gcgccgctac ggcgacaagt
      361 tcgaccagga cgccagggag ttcatgcact acgtggtcga cggcgccgcg cgcatgaagc
      421 agctgatcga ggacctgctc gcgtattcca aggtcggcac gcgcgccaag gatttcggtg
      481 acgccgacgt cgaagcggcg ctgcgccgcg ccatcaccaa cctcaaggcc gcgatcgggg
      541 aaacgggcgc cgcggtcacc tgggacccgc tgcccaaggt gaaggccgac gacgcgcagc
      601 tcgcgcagct gttccagaac ctgatcggca acgcgctgaa gttccgttca gcttccgtgc
      661 cgcgcgtgca cgtttctgtt tctcaaaagg attcagaatg ggaattctcg gtgcgcgaca
      721 acggcatcgg catcgagccg cagtacttcg agcgcatctt catggtgttc cagcgcctgc
      781 acgacaaggc cgaatacccg ggaaccggga tcggcctcgc gatctgcaag aaggtggtcg
      841 agcgccacgg cggccgcatc tgggtcgaat cgcagccggg cgcgggcagc gcctttcact
      901 tcacgctacc gaagggcttc acgctaccga agggcttcaa atccagcgag gcagaggggg
      961 aacgcccatg actgacggcg gatcgccggt cgagatcctg ctggtggagg acaaccccgg
     1021 cgacgagcgc ctgacgcgcg aggcgctgcg cgagggcaag gtctatcaca acctgcactg
     1081 ggtgacggac ggcgtgcagg cgatggagtt cctgcgccgc cagggcaagt acgcaagcgt
     1141 gccgcgcccc gacatcgtgc tgctcgacct caacctgccg aagaaggacg gccgcgaggt
     1201 gctcgaggag atcaagaccg acgaggacct gaagcgcatc ccggtggtgg tgctcaccac
     1261 ctcgaaggcc gaggaggacg tgctgcgcac ctacaacctg cacgccaact gctacgtcac
     1321 caagcccgtc gacctggaga agttcatcgt ggtggtgaaa tcgatcgacg tgttctggct
     1381 cacggtggtc acgctgccgc ccgatgggca cggcaagtag tgcataacgt cctcctcatc
     1441 gaggacaacc ccggcgacgc gcggctcatc gccgagatga tcgccgagca cccgggcgcg
     1501 ccgttcgtgc tgcacaccgc cgatcgcctc tcgctcgggc tcgagcacct gtcggccggc
     1561 agcaccgggc tggtgctgct cgatctctcg ctgcccgaca gcttcgggct cgataccttc
     1621 gccaaggtgt atgcccattc ccccaccgtg ccgatcatcg tgctcaccgg caacgacgac
     1681 cacatgctcg cgctctcggc ggtgaagagc ggcgcgcagg actacctggt gaagggccgc
     1741 ctcgaccgcg agctgctgct gcgctcgatg cagtattcga tcgagcgcaa gcgctaccag
     1801 gtgcagctcg agcatcaggc gaactacgac gcgctcaccg ggctgcccaa ccgcaacctg
     1861 ctgcacgacc ggctcaggca ggcggtgtac gcgcagcgca gcccgcgcaa catcgcggtc
     1921 gtgttcatgg acctcgacca cttcaagttc gtcaacgaca gcctcggcca cggcaccggc
     1981 gacaagctgc tgaaaagcat gggagagcgc ctgcgcgccg tgctgcgcga aggcgatacc
     2041 gtgggccgcg tcggcggcga cgagttcgtg ctgatcctca acgaccagtc gaacgaggag
     2101 gtgatcttcc gcgccatgca gcgcatcagc gccaaggtgt cggagtcgat caccatcgag
     2161 ggcaaggagc tctacgtcac ctgcagcgcc ggcatcagcc tgtacccgca ggacgggcgc
     2221 gacgtcgata cgctgctcaa gaacgccgat gccgcgatgt accgcgcgaa ggagcacggc
     2281 cgcaacaact tccagttcta cacctcggag atgaacgagc gggtgaacga gcgcctgtcg
     2341 ctcgaggccg cgctgcgccg cgcgctcgag cggcgcgagt tcctgctgca ctaccagcag
     2401 aagatcgacc tgaggtcggg cgccatcatc ggcgccgagg cgctggtgcg ctggaaccac
     2461 ccggaatggg cgctggtgcg gccggcgcgc ttcatcccgc tcgccgagga gaccggcctg
     2521 atcgtgccgc tcggcgaatg ggtgctgcac gaggcctgcc gccaggcgcg cgagtggctc
     2581 gaccagggcc tgcggcccgg cgtcgtgtcg gtgaacctgt cggtgcgcca gttcaggcag
     2641 gaggggctgg tgcgcaccgt gtcgcgcatt ctcgaggaga cgcggctcga tgcgtcacaa
     2701 ctcgaaatgg agctgaccga aagcatggtg atgcagaacg tcgaatcggc gatcgccacg
     2761 ctgcagggcc tgaagtcgct cggcgtcgcg ctgtcggtag acgacttcgg caccggctac
     2821 tcgagcctgt cgtacctcaa gcagctgccg ttcgacacgc tcaagatcga ccgctcgttc
     2881 gtgcgcaata tcggcgccgg cgaggacccc gacgaaggcg tgatcgcgca ggcgatcatc
     2941 tcgctcggcc acagcctgcg cctcaaggtg atcgccgagg gcgtcgagac tgatgctcag
     3001 gtgcgcttcc tcaagcgcca cggctgcgac gaggtgcagg gcttcctgta cggcgagccg
     3061 gtggccgccg aggattacgc gaagctgctg gacaaggcga aacgcaaagg aaagcgcgca
     3121 tgagattttt tcttttcatc acattgggaa tcttttcgct ggccggcttt tgcgacgaga
     3181 tcaaagtgct gtcgggcggc gccgcccggg ggttcgtcga gccgctcgcc gcgtcgttcc
     3241 aggcgcacgc ggtgaagctc gaataccaga cgatggggcg cctgatgaag accatggcct
     3301 ccgccgaggc ggcgcgctac gacatgctca tcgtcaccac cgaggtggcg ccgaagctgc
     3361 agctcgcgga cgtaaagccg ctcgcgcgcg tcggcatcgg cgtggcggtc aatgagaagg
     3421 cgccgctacc tgatatttcg acagtcgatt cgtttaaaag gacgctgctc gccgcgaagt
     3481 ccatcgtcta catcaatccg cagaccggga ccagcggcaa atacgtcgtc gaggctttcc
     3541 gcaagctggg gatcgagggc gagatgaggg cgaagaccac gctggtcgac tcgggatacg
     3601 cggtcgcgcc ggtcggccgc ggcgagatcg agctcggcat ccaccagatc agcgagatcc
     3661 tgccggtgaa gggcgtcaag ctggtcgggc cgctgccgcc cgagctgcag aaatacacgg
     3721 tctacgtggc cgctgcgctg ccgggcacgc agaaaaagcc cgccgtggac gcctttatcg
     3781 ctcatctcac cgggaccgag gcacgcagcc gcctggcgca ggcgggatac acttccccgg
     3841 agtgagcgac aaggcacgcc ccgtgatcct cgcggttgac gatgcgaccg acctgctcgc
     3901 gctcatggcg caggcgctgg gtggcgagta cgaggtcatc accgccagcg acgcgggcac
     3961 ggcgatcgag aaggcgttcg gcgagccgcg ccccgacctg atcctgctcg acatcgacat
     4021 gcccgacatc tcgggcttcg aggtgtgccg cgcgctgaag gacgaggcga tcaccaccgc
     4081 gatcccggtc atcttcctga ccggcaggac cgaggcgcag gcgcagctcg aaggcttcga
     4141 gctcggcgcg gtggactacg tcaccaagcc gatcaacgcc gcggtgctca tggcgcgcgt
     4201 gcgcatgcac ctcgcgctca gcaaccagcg ccaggccctc gagcacatgg tgcaggagcg
     4261 caccgggcag ctcgaccgca cgcgcgccga gctgatcaag cgcctgtcgc gcgcgatgga
     4321 gctgcacgag agcgccgcgg tcggcaaccg cgtcatgcgc ctgggccact acgccaaact
     4381 catcgcccag gcggccggcg cgaagcccga gatcgccgag atgatgctga aggccgcgcc
     4441 gctgcacgac atcggcaagc tcggcgtgcc cgcggagatc ctgcgcaagg ccgagaagct
     4501 cagcgtcccc gactgggagc gggtcaagcg ccacccgcag ctcggcgccg agatcatcgg
     4561 cgagcacgac gacccgctgc tcaagctcgc gcgccagctc gccctcgcgc accatgagcg
     4621 ttgggacggc tcgggctacc cgcaagggct gaaggcggag gcgattccgt ggggcggccg
     4681 cgtcatggcg atcgtcgaca gcttcgagtc catgaccagc acccaattct tccgcgacgc
     4741 gattcccgtc gaccaggcgg ccgccgagat cgagaaaggt gcgggcacgc gctacgagcc
     4801 gaagctggtc gaggcgttca gaaaggcgct gccggtaatg aagaaggtgc gcgagaccta
     4861 cgcggacaag ctcggcgacc tgatcaacct ggatttcgcg ccgaaagggg ctgccaagcc
     4921 cgctgcggcg ccggccgccg ctgcccccaa gccgccgcct gcgcctccca agccggccaa
     4981 gccggtcgtc agcgccgcgc agctcgccaa agccgccgcg gcgaagagga aatgagcaag
     5041 ctgttcgaga agctgaaggg cgccgagcgc gagcggctcg agaaggaccg cagcggcgcg
     5101 gaagtggcgc aggcgcgcgc cggcgaggag cggcgcgcgc tcgatctggc gcgcgagcgc
     5161 cagcaggccg aggtcgagct gcggcgcatc gcgcacgcgc gcgccgaagc cgaggccaag
     5221 gcgg
//
LOCUS       DASPXW010000055         2506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_775191, whole genome shotgun sequence.
ACCESSION   DASPXW010000055 DASPXW010000000
VERSION     DASPXW010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775191"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..1064)
                     /locus_tag="VEL04_01335"
     CDS             complement(84..1064)
                     /locus_tag="VEL04_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYT47038.1"
                     /translation="MKRREVLGLGAALAALGVAGTLRAQDAAAWPARPVRLILPFTPG
                     GATDFQGRILCDVLTRHFGQNFFIDYKPGAGSNIGVAEVARSAPDGYTLGWITVASHA
                     INPTLYAKLPFDHIRDFAPVSMIGMFPNLLVVNNALPVHTVAELIALLKREPGKYAFA
                     SSGQGTSLHLSGELFKMMTGTDMLHVPYKGGGPAITDVIAGQVHMTFGNMPTVLPHVK
                     AGRVRAIAVTSAERWFTAPEIPTVAETVPGFLSMSWHGVAFPARTPGAIVDKLAAAIH
                     RALATPEMQERLAAGGSKAMPMSPREFAAFIREDTARWAPAIRASGARVD"
     gene            1085..1423
                     /locus_tag="VEL04_01340"
     CDS             1085..1423
                     /locus_tag="VEL04_01340"
                     /inference="COORDINATES: protein motif:HMM:NF045268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47039.1"
                     /translation="MNKRPHDMGGEPGGPIDTADHGMKFWEKQANALRSTLTAGKVLR
                     LDELRRAAEDLGERYLQLEYFERTTEALLMLRLVDFKPLERIALPDIGELTTSGLTVL
                     IGPRPMSRRW"
     gene            1431..1733
                     /locus_tag="VEL04_01345"
     CDS             1431..1733
                     /locus_tag="VEL04_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47040.1"
                     /translation="MKRVALLAVWSCLAFAQDSGQEALESYKRALEEERRASQQKRSE
                     AEASRGDLGKPVEHKRDAALCERSRQYAQMACGAPYSGRSRSARCTEAKILLQQDC"
     gene            complement(1742..>2506)
                     /locus_tag="VEL04_01350"
     CDS             complement(1742..>2506)
                     /locus_tag="VEL04_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47041.1"
                     /translation="GGATTVDYDGKGRIWVSAPDGVLRFDPDTQKFTDYKTATYKTPN
                     GTGITYGAAGDRNGNGYWAVMIHDIIGVADGRTPAGEKGKSSEILLAPVKSETARVTP
                     EARKVYAAYSQPDFNNPLPWSQGPRRMGTDKNADVLWVGNSWGASLARIDTLTHEVTY
                     VPLPGRMQPYHVHVDSRHRPWTNLWGADRVMRYDPATKTWTAFDLPTRGSEARYISVH
                     EKDGQMQVVLPYFRARKIAVITMRSEAQIQALAKPQ"
BASE COUNT          394 a          832 c          875 g          405 t
ORIGIN      
        1 ccgagctcgt gacgcgcatc gagcgcggcg agctcgagcc ttcgccttca catttgggag
       61 cttgagagaa gtaagaccgg cccctaatcg acccgcgcgc cggaggcgcg gatcgccggc
      121 gcccagcgcg cggtgtcctc gcggatgaag gcggcgaact cgcgcggcga catcggcatc
      181 gccttagagc cgccggcggc gaggcgctcc tgcatctccg gggtcgccag cgcgcgatgg
      241 atcgccgcgg cgagcttgtc cacgatggcg ccgggcgtgc gcgcaggaaa cgcgaccccg
      301 tgccacgaca tcgagagaaa gcccggcacc gtctccgcca ccgtggggat ctccggcgcc
      361 gtgaaccagc gctcggctga agtcaccgcg atggcgcgca ccctgcccgc tttcacgtgc
      421 ggcagtaccg tgggcatgtt gccgaaggtc atgtgcacct ggccggcgat cacgtccgtg
      481 atggccggtc cgccgccctt gtaagggacg tgcagcatgt cggtgccggt catcatcttg
      541 aagagctcgc ccgagaggtg cagcgacgtg ccctgccccg acgaggcgaa ggcgtacttg
      601 ccgggctccc tcttcaggag cgcgatcagc tccgccaccg tgtgcaccgg aagggcgttg
      661 ttgaccacga gcagattcgg gaacatgccg atcatcgaca ccggcgcgaa gtcccggatg
      721 tggtcgaatg gcagcttcgc gtaaagcgtc gggttaatcg cgtgcgaggc gaccgtgatc
      781 cagccgagcg tgtagccgtc gggcgcgctg cgcgcgactt cggcgacgcc gatgttgctg
      841 ccggcgccgg gcttgtagtc gatgaagaag ttctgcccga agtggcgcgt gagcacgtcg
      901 cacaggatcc ggccctggaa gtccgtcgcg ccgccgggcg tgaacggcag gatgaggcgc
      961 accggccggg cgggccacgc cgcagcatcc tgcgcgcgca gcgtccccgc gacaccgagc
     1021 gccgcgagcg ccgcaccgag cccgaggact tctcttcgtt tcaacccggt ttagtataga
     1081 actcatgaac aagcgtccgc acgacatggg cggcgagccg ggcggcccga tcgacaccgc
     1141 cgatcacggc atgaagttct gggagaagca ggcgaacgcc ctgcgcagca ccctcaccgc
     1201 cggcaaggtc ctgcgcctcg acgagctccg ccgcgccgcc gaggacctcg gtgagcgcta
     1261 cctccagctc gaatacttcg agcgcaccac cgaggcccta ctcatgctac ggcttgttga
     1321 cttcaagccc ttagaacgta ttgctctgcc tgacattggc gagctgacga catctggcct
     1381 taccgtactt attggcccgc ggccgatgag ccgccgatgg tgaaccgggg ttgaagcgcg
     1441 tcgcgctgct cgccgtctgg tcctgcctgg cattcgccca ggacagcggc caagaagcgc
     1501 tcgagtcgta caagcgcgcg ctggaagaag agcgccgcgc cagccagcag aagcgcagcg
     1561 aagccgaagc gtcgcgcggc gacctgggca agccggtgga gcacaaacgc gatgccgcgc
     1621 tttgcgagcg cagccggcag tacgcccaga tggcgtgcgg cgcgccctac tcgggccgct
     1681 cgcgcagtgc gcgctgcacg gaggcgaaga tactgctgca gcaggactgc tgagcggatc
     1741 gctactgcgg cttcgccagc gcctggatct gcgcttcgct gcgcatcgtg atgaccgcga
     1801 tcttgcgcgc gcggaagtac ggcagcacga cctgcatctg tccgtctttt tcatgaacgg
     1861 aaatatagcg cgcctcggag ccgcgcgtcg ggaggtcgaa ggcggtccag gtcttggtcg
     1921 ccgggtcgta gcgcatcacg cggtcggcgc cccacaggtt ggtccacggc cggtgccggc
     1981 tgtcgacgtg cacgtggtag ggctgcatgc ggcccggcag cgggacgtag gtcacctcgt
     2041 gcgtgagcgt gtcgatgcgc gcgaggctcg cgccccagga gttgccgacc catagcacgt
     2101 cggcgttctt gtcggtgccc atgcggcgcg ggccctgcga ccagggaagc gggttgttga
     2161 agtcgggctg gctgtaggcc gcgtagacct tgcgcgcttc gggcgtgacg cgcgccgttt
     2221 cggacttcac cggggcgagg aggatctcgg aggacttgcc cttctcgcca gcaggcgtgc
     2281 ggccgtcggc gacgccgatg atgtcgtgga tcatcaccgc ccagtagccg ttgccgttgc
     2341 ggtcgccggc cgcgccgtaa gtgatcccgg tgccgttcgg cgtcttgtag gtggcggtct
     2401 tgtagtcggt gaatttctgc gtgtcgggat cgaagcgcag cacgccgtcg ggcgccgaaa
     2461 cccagatcct gcccttgccg tcgtagtcca ccgtggtggc gccgcc
//
LOCUS       DASPXW010000056         3579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_775346, whole genome shotgun sequence.
ACCESSION   DASPXW010000056 DASPXW010000000
VERSION     DASPXW010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775346"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..1234)
                     /locus_tag="VEL04_01355"
     CDS             complement(86..1234)
                     /locus_tag="VEL04_01355"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYT47042.1"
                     /translation="MKPWLLGALLAFAPLALAQSWGPRTLDELKQETVRRAERNMVPL
                     TGIKAEDAREAMAGLKSLEPDEWAAAWSRIGERYFAQGDYFHAWDIFNVARWPSEKLS
                     PGKAKAYARALEAFQLYGKSLSPAIETVRIPFEGKEIVAYLRLPQGVRPAPLVFGING
                     LDSRKEEVIARADRYLKAGVGVFAIDMPGTGQAPILIDVGAERMFSRALDYLQTRPEL
                     DAKRIVVQGRSWSGYWAAVMAYTEKDRIRGAVVHGVGIHEYYAPDWQKKAFSTREYLF
                     DIYPARAAVYGTKSMEEFLAYGPRLSLVARGMLDKPSAPMLLVNGERDSQQPIADLYL
                     LMKHGDPKDVWVNPEGGHMGRSERWPQGKVLSEVVEPWVLRRLGVVPH"
     gene            complement(1258..1539)
                     /locus_tag="VEL04_01360"
     CDS             complement(1258..1539)
                     /locus_tag="VEL04_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47043.1"
                     /translation="MRKALAACVLVAAAGAAWPQSPTPEKPAAQAPERPATAKEAPLN
                     LRLDNPARFVSRDQAPAEKPLPSLGGTPQTSPRSFERPSPFPKDTNPGI"
     gene            complement(1536..2030)
                     /locus_tag="VEL04_01365"
     CDS             complement(1536..2030)
                     /locus_tag="VEL04_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HYT47044.1"
                     /translation="MSDKRLKELGIQLPAVTPPVANYVNAVRIGNLLFLAGKGPAEVD
                     GMRPQGKLGKDFNVEQGYKFARSVGLALLAVMQAELGSLDRVKRVVKVLGMVNGTPEF
                     TDQPKVINGCSDLFVEVLGERGKHARSAVGMGSLPNGIPVEIECIVEIGGAAPKKTAK
                     KKKK"
     gene            complement(2027..3415)
                     /locus_tag="VEL04_01370"
     CDS             complement(2027..3415)
                     /locus_tag="VEL04_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HYT47045.1"
                     /translation="MNQAREFDASVLDELKALLGERVSTSGAVREHHGKDESYFPYAL
                     PDAVVFVETTEEVRDVVNLCRRHRVPMIPFGVGTSLEGHILAIRGGVSIDLSRMNQVL
                     TVHEEDLDAVVQAGVTRKQLNEYIKHTGLFFPIDPGADATLGGMAATRASGTNAVRYG
                     TMRENVLSLKVVLADGRVLQTSRRAKKSAAGYDLTRLFIGSEGTLGIITEVTVKLYPV
                     QEAMSAAVCAFRSIDGAANTVIQTLQMGIPVARSELLCATTMAALNKHNKLNYRESPT
                     LLLEFHGTQASVVEQAKSVQEIAKDNGGLDFQWATRPEERSKIWEARHNAYFACLQLK
                     PGARALSTDVCVPISRLAECITETEKDIASASLPIPLFGHVGDGNFHLVILIDPANDA
                     EIAEAKALNVRLVRRALAMEGTCTGEHGIGIGKQSYLVEELGEAVELMRDLKRTFDPD
                     NLMNPGKVVSLP"
     gene            complement(3421..>3579)
                     /locus_tag="VEL04_01375"
     CDS             complement(3421..>3579)
                     /locus_tag="VEL04_01375"
                     /inference="COORDINATES: protein motif:HMM:NF039472.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6494 family protein"
                     /protein_id="HYT47046.1"
                     /translation="VGVNSQREIEQAVQKALEAGKLKGGESFSAKMTLELPSLGVKVP
                     FDGEIRLK"
BASE COUNT          551 a         1223 c         1151 g          654 t
ORIGIN      
        1 tcaagcaggg caagcacgag gcgctcgccg acatcctcga atcgatcgag gagctgaagt
       61 actaccgcaa gacggtgatg agcatctaat gcggcacgac gcctagccgg cgcagcaccc
      121 acggttcgac gacctccgag agcaccttgc cctgcggcca gcgctcggag cgacccatgt
      181 ggccgccctc gggattgacc cacacgtcct tgggatcgcc gtgcttcatc agcaggtaca
      241 gatcagcgat cggctgctgg ctgtcgcgct cgccgttgac cagcagcatc ggcgccgagg
      301 gcttgtcgag catgccgcgc gcgacgagcg acaggcgcgg cccgtaggcg aggaactcct
      361 ccatgctctt cgtgccgtac accgccgcgc gcgccggata gatgtcgaac aggtactcgc
      421 gggtcgagaa cgccttcttc tgccaatcgg gcgcgtaata ctcgtggatg ccgacgccgt
      481 gcaccaccgc gccgcggatg cggtccttct cggtgtaggc catgaccgcg gcccagtagc
      541 cgctccagct gcgcccctgt accacgatgc gcttcgcgtc gagctcggga cgggtctgca
      601 ggtagtcgag cgcgcgcgag aacatgcgct cggcgccgac gtcgatcagg atcggcgcct
      661 gcccggtgcc gggcatgtcg atggcgaaca cgccgacgcc cgccttgagg tagcggtcgg
      721 cgcgcgcgat cacctcttcc ttgcgcgagt ccaggccgtt gatgccgaag accagcggcg
      781 ccgggcgcac gccctgcggc agccgcaggt aggcgacgat ctccttgccc tcgaacggaa
      841 tgcgtaccgt ctcgatcgct ggactcaaag attttccata aagttgaaaa gcttcaaggg
      901 cgcgcgcgta agcctttgcc ttgcccggcg agagcttctc gctcggccag cgcgcgacgt
      961 tgaagatgtc ccaggcatgg aaatagtcgc cctgcgcgaa gtagcgctcg ccgatccggc
     1021 tccacgccgc ggcccattcg tcgggctcga ggctcttcag gccggccatc gcctcgcgcg
     1081 cgtcctcggc cttgatgccg gtgagcggca ccatgttgcg ctcggcgcgg cgcacggtct
     1141 cctgcttcag ctcgtcgagc gtgcgcggcc cccaggactg ggcgagcgcc aacggcgcga
     1201 aggcgaggag ggcgccgagg agccacggct tcatttcttt ttctcctttc ccgcctgcta
     1261 gatgcccggg ttggtgtcct tcgggaacgg cgacggccgc tcgaaggaac gcggcgaggt
     1321 ctgcggcgtc ccgccgagcg atggcaatgg cttctcggcc ggggcctggt ctcgtgaaac
     1381 gaaccgggca gggttgtcca gccgcaagtt gagcggggct tccttggccg tcgccggacg
     1441 ctccggcgcc tgcgccgccg gcttctcggg cgtgggcgac tgcggccagg ctgcgcccgc
     1501 tgcggcgacg agtacgcaag ctgcgagcgc ctttctcatt tctttttctt cttcgccgtc
     1561 ttcttcggcg cggcgccgcc aatctcgacg atgcactcga tctcgaccgg gatgccgttg
     1621 ggcagcgatc ccatgccgac cgccgagcgc gcgtgcttgc cgcgctcgcc cagcacctcg
     1681 acgaacagat ccgagcagcc gttgatcacc ttgggctggt cggtgaattc cggtgtgccg
     1741 ttcaccatgc cgagcacctt caccacgcgc ttcacgcggt cgaggctgcc gagctcggcc
     1801 tgcatcaccg cgagcagcgc caagccgacc gagcgggcga acttgtagcc ttgttctaca
     1861 ttgaaatctt tacccagctt cccctgtgga cgcataccgt cgacttcagc aggacctttg
     1921 ccggcgagaa acagcaggtt gccgatgcgc accgcgttga cgtagttcgc caccggcggc
     1981 gtcaccgcgg gcagctggat gccgagctcc ttgaggcgct tgtcgctcat ggcagggaaa
     2041 cgacctttcc ggggttcatc aggttgtcgg gatcgaaggt gcgcttgaga tcgcgcatca
     2101 gctcgacggc ctcccccagc tcttccacca ggtaggactg tttgccgatg ccgatgccgt
     2161 gctcgccggt gcaggtgccc tccatcgcca gcgcccgcct gaccaggcgc acgttcaacg
     2221 ccttcgcctc ggcaatttcc gcgtcgttgg ccggatcgat caggatcacg agatggaagt
     2281 tgccgtcgcc gacatgcccg aacagcggga ttggcagcga cgcgctggcg atgtccttct
     2341 cggtctcggt aatgcactcg gcgagccgcg agatcggcac gcagacgtcg gtcgaaagtg
     2401 cgcgcgcgcc aggcttgagc tgcaagcacg cgaagtaggc gttgtggcgc gcttcccaga
     2461 tcttggagcg ctcctccggc cgggtggccc actggaagtc gaggccgccg ttgtccttgg
     2521 cgatctcctg gaccgatttc gcctgctcga cgacgctcgc ctgggtgccg tggaactcga
     2581 gcagcagcgt cggcgactcg cggtaattca gcttgttgtg cttgttgagc gccgccatgg
     2641 tggtcgcgca cagcagctcg ctgcgcgcca ccgggatgcc catctgcagc gtctggatca
     2701 ccgtgtttgc ggcgccatcg atgctgcgaa aggcgcacac cgccgccgac atcgcctcct
     2761 gcaccggata gagcttgacc gtcacctcgg tgatgatgcc gagcgtgcct tccgagccga
     2821 tgaacagccg cgtcaggtca tagcccgcgg ctgacttctt cgcgcgccgc gaagtctgca
     2881 gcacccggcc atccgccagc accaccttca gcgacagcac gttctcgcgc atcgtgccgt
     2941 agcgcaccgc gttggtgccc gaggcgcggg tcgccgccat gccgccgagg gtggcgtcgg
     3001 cgcccgggtc gatggggaag aacagcccgg tgtgcttgat gtattcgttc agctgcttgc
     3061 gcgtcacgcc ggcctgcacc acggcgtcga ggtcctcctc gtgcaccgtg agcacctggt
     3121 tcatgcgcga caggtcgatc gacacgccgc ccctgatggc gaggatgtgg ccttccagcg
     3181 aggtgccgac gccgaacggg atcatcggca cgcggtggcg gcggcagagg ttgaccacgt
     3241 cgcgcacctc ctccgtggtt tccacgaaca ccactgcatc cggcagcgcg taggggaaat
     3301 acgactcgtc cttgccgtgg tgctcgcgca ccgcgccgga agtcgagacg cgctcgccga
     3361 gcagcgcctt cagctcgtcg agcaccgacg cgtcgaattc tctcgcctgg ttcatgcgat
     3421 ttattttagt ctgatttcgc cgtcgaacgg cacctttacc ccgagcgacg gcagctccag
     3481 cgtcatcttg gcggaaaagc tctcgccgcc tttcagcttt cccgcttcaa gcgctttttg
     3541 aaccgcctgc tcgatctcgc gctgcgagtt gacgccgac
//
LOCUS       DASPXW010000057         2946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_648874, whole genome shotgun sequence.
ACCESSION   DASPXW010000057 DASPXW010000000
VERSION     DASPXW010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648874"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="VEL04_01380"
     CDS             <1..723
                     /locus_tag="VEL04_01380"
                     /inference="COORDINATES: protein motif:HMM:NF023428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47047.1"
                     /translation="VQAARCGCDRAGARPRRGGRRQKPAARPGGAGGVDPHVGRRRAP
                     IYDKSQDGHYNLISALHKSVRGSDPDAALYYLCRMLDAGEAPLYIARRVVRMAIEDVG
                     LADPQALVVANAAKDAYDFLGSPEGELAIAQAVIYVATAPKSNAAYAAYGQAMQVAKE
                     AGSLLPPKHILNAPTRLMQQEGYGRGYAYDHDEPEAFSGQDYFPETLSRRVFYDPPER
                     GFEREIRKRLEYWAKLRKERKP"
     gene            815..1036
                     /locus_tag="VEL04_01385"
     CDS             815..1036
                     /locus_tag="VEL04_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47048.1"
                     /translation="MNKRLDEAIARVKALPEDRQGEIAELLFEFIANEHPDAYLTPEQ
                     IAEIERCMSDDEPYASDEKVRAVFGRLTK"
     gene            1612..2556
                     /locus_tag="VEL04_01390"
     CDS             1612..2556
                     /locus_tag="VEL04_01390"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HYT47049.1"
                     /translation="MRVDRFLEARFPGLAFSHIQRVIRKGQLRVNGRRVEPKDRLEAG
                     QNVRIPPLRLEAPPPRGEQGRDAETRAFLHSITLYEDADVLVLDKPMGLAVQGGSGTV
                     RHVDGMLEVLRDAKGQRPRLVHRLDKDTAGCLLVAKTRFAASALAKSFRSRAARKIYW
                     ALVAGVPKPRQGRISTFLVKEELESDSRMRVARHGEEGASHAVTYYAVVETAARQLAW
                     LSLKPVTGRTHQLRAHCAHIGHPIVGDPKYFSKENWELPGGLQNRLHLLARRIVVPHP
                     RGEKIDVTAQLPPHMVQTWNLLGFDAARYDPIEAAPEE"
     gene            complement(2614..>2946)
                     /locus_tag="VEL04_01395"
     CDS             complement(2614..>2946)
                     /locus_tag="VEL04_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HYT47050.1"
                     /translation="NRLDFITGRAPITVDYAPGAVEIIEQHDGSKLALRKLAPDYDPH
                     DRLAATAYLQERAAQGEIVTGLLFVEPEAEDLHGHLDTVDAPLNALGEAELCPGSAAL
                     AKLNASLR"
BASE COUNT          529 a          941 c         1009 g          467 t
ORIGIN      
        1 gttcaagccg ctcgatgcgg atgcgatcga gcaggtgctc gcccgcgccg aggcgggcga
       61 aggcaaaagc ctgccgctcg acccggaggc gcgggcggcg ttgatccgca tgtcggacgg
      121 cgacgcgcgc cgatctacga caaatcgcag gacggccact acaacttgat ctcggcgctg
      181 cacaaatcgg tgcgcggctc cgatcccgac gccgcgctct attatctctg ccgcatgctg
      241 gacgcgggcg aggccccgct ctacatcgcg cggcgggtgg tgcgcatggc gatcgaggac
      301 gtaggcctcg ccgacccgca ggcactcgtg gtggcgaacg cggcgaagga cgcctacgat
      361 ttcctcggct cgcccgaggg cgagctcgca atcgcgcaag cggtgatcta cgtcgcgacc
      421 gcgccgaaat cgaacgcggc ctatgcggcc tacggccagg cgatgcaggt cgccaaggaa
      481 gcgggctcgc tcttgccgcc caagcacatc ctcaatgcgc ccacccggct gatgcagcag
      541 gagggctacg gccgcggcta tgcgtacgat cacgacgagc ccgaggcttt ctcggggcag
      601 gactattttc ccgagacgct cagccgccgc gttttttacg atccgccgga gcgcggcttc
      661 gagcgcgaga tccggaaacg cctggaatat tgggcgaagc tgcggaaaga gcggaagccg
      721 tagcgccggc ggagccggcg cgtaatccgg gatcccggat tgcgcttcgc tccatgcggg
      781 gtacaggcta tgatcttgga gaaaaggtgc atccatgaac aagcgtctcg atgaagccat
      841 cgcccgtgtg aaggcgctcc cagaggatcg gcagggcgag attgccgagc tgctgttcga
      901 attcatcgcc aacgagcatc cggatgccta tttgacgccg gaacagatcg ccgagatcga
      961 gcggtgcatg tcggatgatg agccgtatgc cagcgacgag aaggtccgag ctgtgtttgg
     1021 ccgtctgacg aaatgacggt ccgctacaag gcgcgcgctc aggcacatca aactattcgt
     1081 gatcatatcg cccaagacga tcagatagcg gcagcgcgcg tcatctcacg catcgaacat
     1141 gcgattgaac ggctcgccgt gtttcctctc tctgcgaggc cggggagcgt gccaggaact
     1201 cgggtccttg ctgtgccggg cctcccttat gtggtgatcc accgcgtacg cggcgattca
     1261 gtcgatatca tcgccgtgct ccacactgcg cgaggtcgac ggagctgacc ggtaacattt
     1321 gcaagtttga gatatcacgt gaaacgcggc ggcacaggac cgaggcagcc gggaggcggg
     1381 cggcagatcg ccgggccgcg caatcatgat cgtggcgcgt cctttacgca gcggcgcgga
     1441 aatcgatttt ccggcagatc gcagcgaagc tggaaggaac gccataagtc tggatacaaa
     1501 tctgacgcgg cgaaaaaact cgaggaatct tcttcgcggc aactggctga accaaaccag
     1561 gcatccacga ccagcgtcca gacgacggcc gttgcgccgg acgaagccgg catgcgcgtc
     1621 gaccggttcc tcgaggcgcg gttccccggc ctcgcgttct cccacatcca gcgcgtcatc
     1681 cgcaaaggcc agctgcgggt gaacggccgc agggtcgagc cgaaggatcg gctcgaggcg
     1741 gggcagaatg tgcgcattcc gccgctgcgg ctcgaagcgc cgccgcctcg cggcgagcag
     1801 gggcgggacg ccgagacgcg ggcattcctg cattccatca cactctacga ggatgccgac
     1861 gtgctggtgc tcgacaagcc gatggggctc gcggtgcagg gcggctcggg cactgtgcgg
     1921 catgtcgacg gcatgctcga ggtgttgcgc gacgccaagg gccagcggcc gcgcctcgtg
     1981 caccgcctcg acaaggatac cgcgggctgc ctgctcgtcg ccaagacgcg gtttgccgcg
     2041 agcgcgctgg cgaagagttt tcgctcgcgc gcggcgcgga aaatctactg ggcgctggtc
     2101 gccggcgtgc cgaagccgcg ccagggccgc atctcgacgt ttctcgtcaa ggaggagctc
     2161 gagagcgact cgcgcatgcg cgtcgcgcgc cacggcgagg agggcgcgag ccatgccgtg
     2221 acctattacg ccgtggtcga gaccgcggct cgtcagcttg cctggctctc gctcaagccg
     2281 gtgaccggcc gcacgcatca actgcgcgcg cattgcgcgc atatcggcca tccgatcgtc
     2341 ggcgatccga aatatttttc gaaggagaat tgggagctgc cgggcggact gcagaaccgg
     2401 ctgcatctgc tcgcgcgccg catcgtcgtg ccgcatccgc gcggggaaaa aatcgacgtg
     2461 accgcgcagc tgccgccgca catggtgcaa acctggaacc tgctcgggtt cgacgccgcg
     2521 cgctacgacc cgatcgaggc cgcgccggag gaataggagg ggcgagaggg ccgagcgaaa
     2581 tttcgcctct ccccgcaagt cgggagaggg agtctaccgc aggctcgcgt tgagcttcgc
     2641 cagcgccgcg gagccggggc acaactcggc ctcgcccagc gcgttgagcg gggcgtccac
     2701 cgtatcgaga tggccgtgca gatcctcggc ctccggctcg acgaaaagca ggccggtgac
     2761 gatctcgccc tgcgcggcgc gctcctgcag ataggcggtg gcggcgagcc ggtcgtgcgg
     2821 atcgtagtcc ggggcgagct tgcgcagcgc gagcttcgag ccgtcgtgct gctcgatgat
     2881 ctccaccgcg cccggcgcgt agtcgacggt gatcggcgcc cgcccggtga tgaagtcgag
     2941 acggtt
//
LOCUS       DASPXW010000058         3148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1784479, whole genome shotgun sequence.
ACCESSION   DASPXW010000058 DASPXW010000000
VERSION     DASPXW010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1784479"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2981
                     /gene="dnaE"
                     /locus_tag="VEL04_01400"
     CDS             <1..2981
                     /gene="dnaE"
                     /locus_tag="VEL04_01400"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HYT47051.1"
                     /translation="ESAERLAGSWSKRFPGRYYIELQRAGHANAEALVSRSVALATRL
                     ALPVVATHPVQFLAPGDFKAHEARVCIAQGYVLGDQRRPRRFTPEQYFKSQEEMARLF
                     ADMPQALDNAVEIARRCNLEIELGRSRLPAFPTPPGVTVDDYLKSQSQAGLERRVAGS
                     LLQTAQGARYRERLDFELRTIVQMGFAGYFLIVADFISWARTNGVPVGPGRGSGAGSL
                     VAYALGITDLDPLRYDLLFERFLNPERVSMPDFDIDFCQDGRDRVIDYVRRKYGADSV
                     SQIATFGTMAARAVVRDVGRVLDLGYNFCDQLAKLIPVQPGRTITLEMARRMEPLLAE
                     RARKEDEVKELLTLAEKLEGLVRNVGMHAGGVLIAPGRLTDYCALYAAEGTANVISQL
                     DKDDVEAVGLVKFDFLGLTTLTILELAARYVRELGDRDFSLEKISLDDADTYQLLSSG
                     NTTAVFQLESRGMRDLIKRARPDRFEDVIALVALFRPGPMELIPEFIARKRGEQRVEY
                     LDKRLEPILAPTYGIMVYQEQVMQIAQVIGGYTLGSADLLRRAMGKKKPEEMAQQRDI
                     FVAGAQKNGLARLKAMQLFDLMEKFAGYGFNRSHAAAYALLAYHTAYMKAHHAAAFSA
                     ANLSAVMDDTDKVRQLHEDTLANGLVILPPDINASQHRFVPVDRSTVRYGLGAVRGTG
                     EAAISALLEARRAAPFDSLFDLCRRVDKRIVNRRVVEALVRAGAFDSLDAQRARLLAS
                     VGRSLEAAEQAERQGSQTSLFGEAAAPRGEHATVEAAPWDLKQTLMEEKSALGFYLSG
                     HLFSVYETNLAGFARTPLARLAASEQRVWMAGIVASARSQMTRRGRMMVVMLDDGTAQ
                     VEISVFNELYDKHRDKLKEDALLIVAGKVQRDDFSGGLRVAADELLDLDALRGRYGAR
                     LRIAMNGAADAKRLQQVLAPYRASGQGACEVVVRYENERAGCEVVLGEAWRVRPDSRL
                     LAELSEWLTPQNVQLVYTSA"
BASE COUNT          512 a         1041 c         1085 g          510 t
ORIGIN      
        1 ccgagtcggc cgagcgcctt gcaggcagct ggtcaaaacg ctttccgggc cgctactaca
       61 ttgagctgca gcgcgcgggc cacgctaatg ccgaggccct ggtttcgcgc tcggtcgcgc
      121 tcgcgacacg gctcgccctt cccgtcgtag cgacccaccc cgtgcagttc ctcgcgcccg
      181 gggacttcaa ggcgcacgaa gcgcgcgtct gcatcgctca gggctacgtg ctcggcgacc
      241 agcgcaggcc gagacggttc acaccggagc agtatttcaa gagccaggag gaaatggcgc
      301 gcctgttcgc cgacatgccc caggcgctcg acaacgcagt cgagatcgcg cggcgctgca
      361 acctcgagat cgagctgggc aggagccgcc tgccggcgtt cccgaccccg cccggagtga
      421 cggtcgacga ttatctgaag agccagtcgc aggcgggact ggagagaagg gtcgcgggca
      481 gcctgttgca gacggcccag ggggcgcgct atcgcgagcg gctcgacttc gagctgcgca
      541 ccatagtcca gatgggcttc gccgggtact tcctgatcgt cgccgatttc atcagttggg
      601 cgcgcacgaa cggcgtcccg gtcgggccgg gccgcggctc gggcgccggg tcgctggttg
      661 cctacgcgct tggcatcacc gatctcgacc cgctgcgcta cgacctgctg ttcgagcgct
      721 ttctcaatcc cgagcgcgtg tcgatgccgg acttcgacat cgatttctgc caggacggcc
      781 gcgaccgcgt catcgactat gtgcgccgca agtatggcgc cgacagcgtc tcgcagatcg
      841 ccaccttcgg caccatggcg gcgcgcgccg tggtgcgcga cgtggggcgc gtgctcgatc
      901 tcggctacaa cttctgtgac cagctcgcga agctgatccc ggtgcagccc ggccggacca
      961 ttaccctcga gatggcgcgc cgcatggagc cgctgctagc cgagcgggcg aggaaggaag
     1021 acgaggtgaa ggagctcctc acgctcgccg agaagctcga ggggctggtg cgcaatgtcg
     1081 gcatgcacgc cgggggcgtg ctgatcgcgc ccggccggct gaccgactac tgcgcgctgt
     1141 acgcagccga gggcacggcg aacgtgattt cccagctcga caaggacgat gtcgaggcgg
     1201 tcgggctggt gaaattcgat ttcctcggcc tgaccacgct caccatcctg gagcttgccg
     1261 cgcggtacgt gcgcgagctc ggtgaccgcg acttttcact ggaaaagatt tccctggatg
     1321 acgcggatac ctaccagctt ctctcctcgg gcaacaccac cgcggtgttc cagctggaat
     1381 cgcgcggcat gcgcgatctc atcaagcgcg cccgcccgga ccgcttcgag gacgtgattg
     1441 cgctggtcgc gctgttccgc cccgggccga tggagttgat cccggaattc atcgcgcgca
     1501 agcgcggcga gcagcgcgtc gagtacctcg acaagcgcct ggagccgatt cttgcgccga
     1561 cttacggcat catggtgtac caggagcagg tgatgcagat cgcgcaggtg atcggcggct
     1621 acacgctggg cagcgccgac ctgctgcgcc gcgcgatggg caagaaaaaa cccgaggaaa
     1681 tggcgcagca gcgcgacatc ttcgtcgccg gcgcgcagaa aaacggtctc gcgcgcttga
     1741 aggcgatgca gctcttcgac ctgatggaga agttcgcggg ctacggcttc aaccgctcgc
     1801 atgccgcggc ttatgcgctg ctcgcctatc acacggccta catgaaagcg caccacgccg
     1861 cagccttcag cgccgcgaac ctttcggccg tgatggacga taccgacaag gtgcgccagc
     1921 tccacgagga caccctggcg aatggcctgg tcatcctgcc tcccgacatc aacgcctcgc
     1981 agcatcgctt cgtcccggtg gatcgcagca cggtgcgcta cggcctcggc gcggtgcgcg
     2041 gcaccggcga agcggcgatc tcggcgctgc tggaggcgcg ccgcgcggcg ccgttcgaca
     2101 gcctgttcga tctctgccgc cgcgtggaca agcgcatcgt caaccggcgc gtggtcgagg
     2161 cgctggtgcg cgccggcgcc ttcgattcgc tcgatgcgca acgcgcgcgg ctgctcgcct
     2221 cggtcgggcg ctcgctcgag gcggccgagc aggccgagcg ccaggggtcg cagaccagcc
     2281 tgttcggcga ggcagccgca ccgcgcggcg agcacgccac cgtcgaggcg gcgccgtggg
     2341 atctgaagca gaccttgatg gaggaaaaga gcgcgctcgg gttttacctg tccgggcatt
     2401 tgttctcggt ctatgaaacg aacctcgccg gcttcgcgcg cacgccgctc gcgcgcctcg
     2461 cggcctccga gcagcgggtc tggatggcgg gaatcgtcgc ttcggcgcgc agccagatga
     2521 cgcgccgcgg ccgcatgatg gtggtgatgc tcgacgacgg caccgcgcag gtcgagatct
     2581 cggtgttcaa cgagctctac gacaagcacc gcgacaagct gaaagaggac gcgctgctga
     2641 tcgtcgccgg caaagtgcag cgcgatgatt tttccggcgg gctgcgcgtc gcggcagacg
     2701 agctgctgga tctcgatgcg ctgcgcggtc gctacggtgc gcggctgcgg atcgcgatga
     2761 acggcgcggc cgacgccaag cgcctgcagc aggtcctcgc gccgtatcgc gcgagcgggc
     2821 agggtgcctg cgaggtggtg gtgcgctatg aaaacgaacg cgccgggtgc gaagtggtac
     2881 tgggcgaggc gtggcgcgtg cggccggaca gccgcctgct cgccgagcta tccgaatggc
     2941 tgacgccgca gaacgttcag ctcgtctata cctcggccta gcgagccggc tcgcgcgcgt
     3001 tgcgcatgaa catgcgccgg aaatggcgat accagggatt aacaatccgc tcgagcgggt
     3061 ccagcctgcg cttctcggcg aggaagctct tcaatcgcgg gtagcaggct tcgatctggg
     3121 cgcgcgtggc ctcaggggtg caggcgat
//
LOCUS       DASPXW010000059         3447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_20738, whole genome shotgun sequence.
ACCESSION   DASPXW010000059 DASPXW010000000
VERSION     DASPXW010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20738"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1884)
                     /locus_tag="VEL04_01405"
     CDS             complement(<1..1884)
                     /locus_tag="VEL04_01405"
                     /inference="COORDINATES: protein motif:HMM:NF006196.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aldolase/short-chain dehydrogenase"
                     /protein_id="HYT47052.1"
                     /translation="MKSLWSDAEAAKFEAQFPGPLGVRVYTSRLLGRDKSLVLHGGGN
                     TSVKLREKNLFGEPEEVLYVKGSGWDLETIEPAGFTPVALGYVRRLAGLAKLPDPQMV
                     NELNTHMLRAGAPSPSVETILHAILPHPYVDHTHADAVLSVSNAPGGEERIREIYGDR
                     VVVIPYVMAGFDLAAYCAREFPKQARGNTAGMVLLSHGIFSFGADAQESYERMIELVS
                     MAERYLEKKQAWQVGFADGKAPGIKREEIVALRRSIADCAGFPVILKLNDGLKFAGFA
                     RHGELARLSQKGPATPDHLLFTKPVPMLGRDVAGYARKYREYFERQAPQAKEKKTMLD
                     AAPRMVLDPAFGFAAVGRTARDAAIVEDLYDHTIDVILRAEALGGWRALDERHIFDIE
                     YWDLEQAKLRKAGAPPPFTGEVAIVTGAASGIGKACAEAFLKRGAAVVGLDRNPAIET
                     LWQRADFLGITCDLTDSKAIENSLDEAVRRFGGVDMLVLNAGIFPSSQPLQEIAAETW
                     KQTMSVNVEAQLRVMQLTHPLLRLAPRGGRIVVIGSRNVSAPGPGAAAYSASKAALNQ
                     LARVAALEWAKDGIRVNSLHPDAVYDTGLWTEALLAARAKAYNLSVEQYKTKNLLKTE
                     VSSK"
     gene            1909..2328
                     /locus_tag="VEL04_01410"
     CDS             1909..2328
                     /locus_tag="VEL04_01410"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYT47053.1"
                     /translation="MVRIELMRWEQARTIAGPIRFSIFVGEQHVPPGIELDDKDADCI
                     HAIAYDEAGKAVGTGRLLPDGHIGRMAVVKDWRRRGVGAAILEALTEEARKRGHAEVV
                     LSAQLQALEFYRGQGFTAEGKVYEEAGILHQLMRRRI"
     gene            complement(2325..3008)
                     /locus_tag="VEL04_01415"
     CDS             complement(2325..3008)
                     /locus_tag="VEL04_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HYT47054.1"
                     /translation="MCLIVLAWRVHPRFPLVVAANRDEFHARPAAAASFWSDRPGILA
                     GRDLEALGTWMGVSRAGRFAAVTNYRGAREPRAAESRGALVSRFLADGPAPAEYMTGL
                     RENSGLYSGFNLLAADRNNLWWISNRDGAPRALAPGIYGLGNLLLDSPEVEPAKARLR
                     GALEPAPAVDALFTLLAASRILNQEYGTRCSTVMLRAEDDRIRYAERSFAPDGAEGET
                     LQFDFPCSS"
     gene            complement(2971..>3447)
                     /locus_tag="VEL04_01420"
     CDS             complement(2971..>3447)
                     /locus_tag="VEL04_01420"
                     /inference="COORDINATES: protein motif:HMM:NF013916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47055.1"
                     /translation="QYEHQSIRNLVQLIDTGNRYGIPTMAVTGVGADMKRDERYFGLA
                     TRIAAELGAHYVKSYFVEPGFERVCAGCPVPIVIAGGKKLPELDALTMAYRAIDQGAA
                     GVDMGRNIFQSDDPIAMIQAVGAVVHQKEKPEKAHQLYLQIKARNVPDRSRLARSS"
BASE COUNT          561 a         1147 c         1158 g          581 t
ORIGIN      
        1 cttcgatgag acctcggtct tgaggaggtt cttcgtcttg tattgctcca cgctcaggtt
       61 ataggccttg gcgcgcgccg cgagcagagc ctcggtccac agccccgtgt cgtagaccgc
      121 gtccggatgc agcgagttga cgcggatgcc gtccttggcc cattcgagcg ccgcgacgcg
      181 cgcaagctgg ttgagggccg ccttggaggc cgaataggcc gccgcgccgg ggcccggcgc
      241 ggacacgttg cgcgagccga tcaccacgat gcgcccgccg cgcggcgcca gcctgaggag
      301 cggatgggtg agctgcatca cgcgcagctg cgcctcgaca ttgaccgaca tggtctgctt
      361 ccaggtctcg gcggcgattt cctgcagcgg ctgggaggag gggaagatgc cggcgttcaa
      421 caccagcatg tcgaccccgc cgaagcgccg cactgcttca tccaggctgt tttcaatggc
      481 tttcgaatcc gtgagatcgc aggtgatgcc aagaaaatcg gcgcgctgcc acaaggtctc
      541 gatggccggg ttgcggtcga ggccgacgac tgcggcgccg cgcttgagga acgcctcggc
      601 gcaggccttg ccgatcccgg aggcggcgcc ggtgacgatc gctacctcgc ccgtgaacgg
      661 cggcggcgcc ccggccttgc gcagcttggc ctgctcgagg tcccagtact cgatgtcgaa
      721 gatgtggcgc tcgtcgagcg cgcgccagcc gccgagcgcc tcggcgcgca ggatcacatc
      781 gatggtatgg tcgtagagat cctcgacgat cgccgcgtcg cgcgccgtgc gacccaccgc
      841 cgcgaagccg aacgccggat cgagcaccat gcgcggcgcc gcatcgagca tggtcttctt
      901 ttccttcgcc tgcggcgcct ggcgctcgaa gtactcgcgg tacttcctcg cgtagccagc
      961 gacgtcgcgt cccagcatcg gcacaggctt ggtgaaaagc aggtgatccg gcgtcgccgg
     1021 tcccttctgc gacagccgcg caagctctcc atgcctcgca aatccggcga atttgaggcc
     1081 atcgttgagc ttcaggatca ccggaaaacc ggcgcagtcg gcgatcgagc gccgtaatgc
     1141 gacgatctcc tcgcgcttga taccgggcgc tttcccatcc gcgaagccga cctgccaggc
     1201 ctgcttcttc tcgaggtagc gctcggccat cgacaccagc tcgatcatgc gctcgtagga
     1261 ctcctgcgcg tcggcgccga acgagaagat gccgtgcgag agcagcacca tgccggcggt
     1321 gttgccgcgt gcctgcttcg gaaattcccg cgcgcagtac gcggcgaggt cgaacccggc
     1381 catgacgtac gggatcacca ccacgcggtc gccgtagatc tcgcgaatcc gctcctcgcc
     1441 gccgggcgcg ttcgacacag agagcaccgc atccgcgtgg gtgtgatcga cataggggtg
     1501 cggcaggatg gcgtgcagga tcgtctcgac cgaaggcgag ggcgcgccgg cgcgcagcat
     1561 gtgggtgttc aactcgttca ccatctgcgg atcgggcagc ttcgccagcc cggcgagccg
     1621 gcgcacgtag ccgagcgcga ccggggtgaa gccggccggc tcgatggtct cgaggtccca
     1681 gccgctgccc ttgacgtaga gcacctcctc gggctcgccg aacaggttct tctcgcgcag
     1741 cttcaccgag gtattgccgc cgccgtgcag caccagcgac ttgtcgcggc cgagcagccg
     1801 cgaggtatag acgcgcacgc cgagagggcc ggggaactgc gcttcgaact tcgccgcctc
     1861 ggcgtcgctc cagagacttt tcatcgccgc ggattatact ttccgctgat ggtccgcatc
     1921 gaactgatgc gctgggagca ggcgcgaacc atcgcgggtc cgatccgctt ctccattttc
     1981 gtcggcgagc agcacgtgcc gcccggaatc gagctcgacg acaaggacgc cgactgcatc
     2041 cacgccatcg cctacgacga ggccggcaag gcggtcggca ccggccgcct gcttccggac
     2101 ggccatatcg gccgcatggc ggtagtaaag gactggcgcc gccggggtgt cggcgccgcg
     2161 atcctggagg cgctcacgga ggaggcgcgc aagcgcggcc atgcggaggt cgtgctttcc
     2221 gcgcaattgc aggcgctgga gttctatcgc ggccagggct tcaccgcgga aggcaaggtt
     2281 tatgaggaag ccggcatcct tcaccagctg atgcgtcggc ggatctagct gctgcaggga
     2341 aagtcaaact gcagcgtttc gccttcggcg ccgtcgggtg cgaagctgcg ctcggcatag
     2401 cgaatccgat cgtcttcggc gcgcagcatc acggtcgagc agcgcgtgcc gtactcctgg
     2461 ttgagaatcc gcgatgccgc gagaagcgtg aacagcgcat cgactgcggg cgccggctcg
     2521 agcgcgccgc gcaggcgcgc cttcgccggc tcgacttccg gggaatcgag cagcagattt
     2581 cccagtccat agatgccggg tgcgagcgct cgcggcgcgc cgtcgcggtt cgagatccac
     2641 cacagattgt tgcggtcggc cgcgagcaga ttgaagccgc tgtaaaggcc cgaattctcc
     2701 cgaagacccg tcatgtattc tgccggcgcc gggccatcgg cgagaaagcg cgataccagc
     2761 gcgccgcgcg attcagccgc ccgcggctct cgagcacccc ggtagttcgt gaccgcggcg
     2821 aatctgccgg cacgggagac gcccatccag gtaccgagcg cctcgagatc gcggccggcg
     2881 aggatccctg ggcgatcgct ccagaacgag gcagcggctg ccgggcgggc gtggaactcg
     2941 tcgcggttgg cggcgacgac cagggggaac ctaggatgaa cgcgccaggc gagaacgatc
     3001 aggcacattc ctggccttga tctgcagata gagctggtgc gccttctccg gcttctcttt
     3061 ttggtggacc accgcgccga ccgcctggat catggcgatc ggatcgtcgg attgaaagat
     3121 attgcgtccc atatcgacgc cggctgcgcc ctgatcgatc gccctgtagg ccatggtcag
     3181 ggcgtcgagc tcgggcagct tcttgccgcc tgcgatgacg atgggcaccg ggcaaccggc
     3241 gcagacgcgt tcgaaacccg gctccacgaa gtagctcttg acgtagtgcg cgcccagctc
     3301 cgccgcgatg cgcgtcgcca ggccgaaata gcgctcgtcc cgcttcatgt cggcacccac
     3361 gcccgtgact gccatggtcg ggatgccgta gcggttgccg gtgtcgatca gctggaccaa
     3421 gttcctgatc gactggtgct cgtactg
//
LOCUS       DASPXW010000060         7706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_271695, whole genome shotgun sequence.
ACCESSION   DASPXW010000060 DASPXW010000000
VERSION     DASPXW010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7706
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271695"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="VEL04_01425"
     CDS             complement(<1..832)
                     /locus_tag="VEL04_01425"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYT47056.1"
                     /translation="MEVFDVFLLEAVLNGLLLGGLLALLSLGLNLVFGVIDVVWICYA
                     ELIMCGMYAVYYLHVQYHWPLFYAMPAAVIVVAALGAALHQFVIRPVLSAEPINQLLV
                     TGGVLFFLQAAATLVFGIEFRNLGVTLPSVRLGEISFSGARLIAFGVALAGMVGLYLF
                     LSKTYLGTAIRAISQDRAIMPLMGVSPARIFAVTSALAGLAACLLVLQYDVHPAIGLS
                     FGPITFLICVLGGLGSMAGGFIAAFVFAQFISVGGFFFHLEWGYVIAFVFFIAMMFWR
                     P"
     gene            complement(832..1536)
                     /locus_tag="VEL04_01430"
     CDS             complement(832..1536)
                     /locus_tag="VEL04_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT47057.1"
                     /translation="MLTLSKVSAAYGSFQALFDVSIDVKAGESVAVIGPNGAGKTTLL
                     RVISKLIPATGGEIRMQDESLAAVPPHEVVARGIAQVPENRRLFPRLTVEENLRMGAY
                     PAACRKKFPERIEFVYGLFPRLKERGRQLAGTLSGGEQQMCAIGRALMSGPKLLLLDE
                     PSAGLAPVLVQSIMELVRRICAEGYTVLIVEQNIRQVLKVVGRAYLLEAGRIKLSGSS
                     EQFLSSEEIRKAYLGL"
     gene            complement(1523..2260)
                     /locus_tag="VEL04_01435"
     CDS             complement(1523..2260)
                     /locus_tag="VEL04_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT47058.1"
                     /translation="MFLEVSKLTKAFGGFTAVNGVSFEVAEGEFLGLIGPNGSGKSTI
                     FNLVAGALRPSAGAVRFRGAEIAGLPAYRVAHAGIARTYQIPRPFRKLSVIENVALAA
                     FYGSNEKLDRQKAFALAAESLRLVNLPVSESLRVEGLGAAGLKKLELARALATQPKLL
                     LADESLGGLDHAEMEQAAEMLAAIRAERGITIVWIEHIMGVLMRLVDRCVVLDHGVVI
                     ARGRPAEVARDPKVIEVYLGADADAYA"
     gene            complement(2261..3547)
                     /locus_tag="VEL04_01440"
     CDS             complement(2261..3547)
                     /locus_tag="VEL04_01440"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYT47059.1"
                     /translation="MRNTRILAAMALCAFAALSQAQQKEIKIGFIYDVTGPFAGGGSE
                     PAQLGTKAIVDYFNEKGGVEGYRINALFVDAQSKVDVAINEMTRLIDQEKVDIIAGIY
                     SSAQCVPMVQQADARKKFVWVNVCIATAVLKDRKLQYVFRPQAHSDQFGAWSCDYLNY
                     YAKSKFGVEPKNLRVAIIHEDGPYGVGVASGNEAQCRKHGLDIVLKEGYSATAPDLSS
                     LVTKLKRARPDVLLHTGYNPDITLFLRQAKEQGLRFKAVIGHGAGHSQIDKLRQTFGD
                     DVNYFHTIDPAAAQLLDPKTLKPGVWAMTQEMVKRYQAVKGPGELPPHVSMGFNNTWI
                     LLNDVLPRAIRKYQGIDSEALRKAALDTDIPVGGTMQGYGVKFFPPGHPLAGQNERAF
                     PVVIQFVGGKGSIVWPKEIQTREPVLPFPKGHAYAP"
     gene            3616..4584
                     /locus_tag="VEL04_01445"
     CDS             3616..4584
                     /locus_tag="VEL04_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYT47060.1"
                     /translation="MHSALIAVLCALLALLPGTAAAAYPERVITLLVAYPPGGGTDVV
                     ARAIAPFIEKYLGGGAKLLIVNRSGAGGEVGMGALANAPADGYTIGFVNTPPLMTIPI
                     ERAAIFGGPQRFELLGNIIDDPCNFAVHADTPVKNLQQLAAWARANPGKLTVGTTGVG
                     SDDHLLMLMFERAARVKLTHVPFKGSSEVRAAIMGKQIDVAAMNVGEALQSIRGGAPM
                     RNLGQFGPARSNLAPDLPTGREQGFPFEMVALRGMAAPKGMPADILERLVKAVAQAAA
                     DPEFQAQAVKVFAPLRYLAPAQFDAEIRAGDAQFRQLWRELPWTDK"
     gene            complement(4630..6303)
                     /locus_tag="VEL04_01450"
     CDS             complement(4630..6303)
                     /locus_tag="VEL04_01450"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYT47061.1"
                     /translation="MACDLLIRNGSLIDGTGAPARRADVAVAGGRIVEIGSISGASRK
                     TIDASGLVVAPGFIDPHTHYDAQICWDGAVTPSSWHGVASVVMGNCGVGIAPCRPEAR
                     EIAMRDLVNVEAIPFEVLDAGITWDWESFPQYMDAAARRKPSLNLAFLAPLTPFRHYV
                     MGEASMERGATESELSKISFLLGEAVDAGAFGFSSTLLNQHMGYGARPLACRNASRAE
                     IKAYCNVLRDRGKGSIEYAMTRQIAVLEEPEVELLGFMLEESRRPVTFIAMFDRDDLP
                     EAARETLRKVRHLIAKGARPQTSPLPLTREVNMRNPFGFAGFPSWKRAFEDKSKAAQA
                     AVYRDPDFRKQFREDLKRPASFGNWARITLHEVRSPELKKWEGKTVAEIAAEQGKDGV
                     DALLDITLQDDLENEFTIQSYNNRVDRMAEILNDRSVLLGLGDGGAHLDMLCDSGYPT
                     YVLGTWVRERKVLTIEEAVRRMTSDPADFFGIRDRGRLKPGLAADIAIFDPATVGSGG
                     RPERLYDLPGGAKRMVMRSRGIEYTIVNGELTWEKGALTGAAAGKVLRS"
     gene            6391..7368
                     /locus_tag="VEL04_01455"
     CDS             6391..7368
                     /locus_tag="VEL04_01455"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYT47062.1"
                     /translation="MNTIRRTVVLALAALALLPRIGRGQRALRRIAWFGVGLPETVLP
                     YLGALQAGLRDSGWEEGRNLTIDRYSSTRAPEDFERIVREIVDAKPEVVVTQEFATLA
                     MLRFAASVPVVFGFSGDPVEAKLVKSLARPGTNFTGISYLASELVGKRIEFLKEALPG
                     IRRIGILARPQHPGEHGERTASEDAARKLGLAVAYFPIRDPAEIDQAFVAMRDAKCDA
                     LVVFPDFTMFTNRERLARLAAAARLPAVSGWSSFADSGLLFNYGPNLRDIYRSLAPFV
                     DRILRGAKPEELPVELPRSIELVVNLRTARDLGIKVPQSILVRADRVIE"
     gene            7365..>7706
                     /locus_tag="VEL04_01460"
     CDS             7365..>7706
                     /locus_tag="VEL04_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47063.1"
                     /translation="MTKRRAFLAVIGCSALAASLKSFAQQPSGKGHRIGFLGSGSAVA
                     VSKPLEEFRAGLRDLGYVEGRNIAIEYRWGDGKFERLPGLAAELIRLKIDLIAVWGTP
                     AALAAKRATSTL"
BASE COUNT         1281 a         2596 c         2545 g         1284 t
ORIGIN      
        1 gcggccgcca gaacatcatc gcgatgaaga acacgaaggc gatgacgtag ccccactcga
       61 ggtggaagaa gaacccgccc accgagatga actgcgcgaa cacgaaggcg gcgatgaagc
      121 cgccggccat gctgccgagg ccgcccagca cgcagatgag aaaggtgatc ggcccgaagg
      181 acaggccgat cgccggatgg acgtcgtact gcagcaccag caggcaggcg gcgagcccgg
      241 cgagcgccga ggtgacggcg aagatgcgcg ccgggctcac gcccatcagc ggcatgatcg
      301 cccggtcctg gctgatggcg cgaatcgccg ttcccagata cgttttgctt aaaaagagat
      361 acaggcccac catccccgcc agcgcgacgc cgaaggcgat gagccgcgca ccggagaacg
      421 agatctcgcc gaggcgcacc gagggcagcg tgacgccgag gttcctgaac tcgatgccga
      481 acaccagggt cgccgccgcc tgcaggaaga acagcacgcc gccggtcacc agcagctggt
      541 tgatcggctc ggcggagagc accggccgga tcacgaactg gtgcagcgcc gcgccgagcg
      601 ccgcgaccac gatcacggcc gcgggcatcg cgtagaagag cggccagtgg tactgcacgt
      661 gcaggtagta caccgcgtac atgccgcaca tgatcagctc cgcgtagcag atccacacca
      721 cgtcgatgac cccgaagacc aggttgaggc cgagcgaaag cagcgcgagc agcccgccga
      781 gcagcaggcc gttcaagacg gcctccaaca ggaagacatc aaagacttcc atcacagtcc
      841 caggtaggcc ttgcggattt cctcagaaga caggaattgc tccgagcttc cggagagctt
      901 gatgcggccg gcctcgagca ggtaggcgcg gccgaccacc ttcagcacct ggcggatgtt
      961 ctgctcgacg atcagcaccg tgtagccctc ggcgcagatg cgccgcacca gctccatgat
     1021 cgactgcacg agcaccggcg cgaggcccgc cgagggctcg tcgagcagca gcagcttcgg
     1081 ccccgacatc agcgcgcggc cgatggcgca catctgctgc tcgccgcccg agagcgtgcc
     1141 ggcgagctgg cggccgcgct ccttcaggcg cggaaacaag ccataaacga actcgatcct
     1201 ctccgggaat ttttttctgc aagcagcagg gtaagcgccc atccgcaggt tttcctcaac
     1261 agtaagccgc ggaaacaggc gccggttctc cggtacctgc gcgatgccgc gcgccaccac
     1321 ttcatggggc ggcaccgcgg cgagcgactc gtcctgcatg cgaatctcgc cgccggtggc
     1381 cgggatcagc ttcgagatca cgcgcagcag cgtcgtcttg ccggcgccgt tcgggccgat
     1441 caccgcgacc gattcgccgg ccttcacgtc gatgctgacg tcgaacagcg cctggaagct
     1501 gccgtaggcc gcggaaacct tgctaagcgt aagcatcggc gtcggcgccg agatagacct
     1561 cgatcacctt cggatcgcgc gccacctcgg cgggcctgcc gcgcgcgatc accacgccgt
     1621 ggtcgagcac cacgcagcga tcgaccaggc gcatcagcac tcccatgatg tgctcgatcc
     1681 agacgatggt gatgccgcgc tcggcgcgga tcgcggcgag catctccgcc gcctgctcca
     1741 tctcggcgtg gtcgaggccg cccagcgact cgtcggcgag cagcagcttc ggctgcgtgg
     1801 cgagcgcccg cgcgagctcc agcttcttca gccccgctgc ccccagccct tccactctca
     1861 gagattcgct gactggaaga ttgaccagcc ggagcgactc ggcggcaagg gcaaacgcct
     1921 tttgccgatc gagcttttca ttgctgccat agaaagcggc gagcgcgacg ttctcgatca
     1981 cgctcagctt cctgaacggg cgcgggatct ggtaggtgcg cgcgatcccg gcgtgcgcga
     2041 cgcggtacgc cggcagcccg gcgatctcgg cgccgcgaaa gcgcaccgct ccggcgctcg
     2101 gccgcagcgc gccggcgacg aggttgaaga tggtgctctt gcccgagccg ttcgggccga
     2161 tgaggccgag gaactcgccc tcggccacct cgaatgaaac cccgtttacg gcggtaaacc
     2221 cgccgaaggc tttcgtgagt tttgagactt cgagaaacac ctatggcgcg tacgcatgac
     2281 ccttcgggaa cggcagcacc ggctcgcgcg tctggatctc cttcggccag acgatgctgc
     2341 ccttgccgcc gacgaactgg atcaccaccg ggaaggcgcg ctcgttctgc ccggcgagcg
     2401 ggtgcccggg cgggaagaac ttgacgccgt agccctgcat cgtgccgccg accgggatgt
     2461 cggtgtcgag cgcagccttg cgcagcgcct cggagtcgat gccctggtat ttgcggatgg
     2521 cgcgcggcag cacgtcgtta agcaggatcc aggtgttgtt gaagcccatc gagacgtgcg
     2581 gcggcagctc gcccggcccc ttcaccgcct ggtagcgctt caccatctcc tgcgtcatcg
     2641 cccagacgcc cggcttgagc gtcttcggat cgagcagctg cgcggcggcc gggtcgatgg
     2701 tgtggaagta gttgacgtcg tcgccgaagg tctggcgcag cttgtcgatc tggctgtggc
     2761 cggcgccgtg gccgatcacc gccttgaagc gcaggccctg ctccttcgcc tggcgcagga
     2821 acagcgtgat gtccgggttg tagccggtgt gcagcagcac gtcgggccgc gcgcgcttca
     2881 gcttggtgac caggctggag aggtctggcg cggtggccga gtagccctcc ttgaggacga
     2941 tgtcgaggcc gtgcttcctg cactgcgcct cgttgccgct cgccacgccg acgccgtagg
     3001 ggccatcctc gtggatgatc gccacgcgca ggttcttcgg ctcgacgccg aacttggatt
     3061 tcgcgtagta gttgaggtag tcgcacgacc aggcgccgaa ctggtcgctg tgcgcctgcg
     3121 ggcggaagac gtactgcagc ttccggtcct tcaacaccgc cgtggcgatg cagacgttga
     3181 cccagacgaa cttcttgcgc gcgtcggcct gctgcaccat cggcacgcac tgcgcgctag
     3241 agtagatgcc agcgatgatg tcgaccttct cctggtcgat gaggcgcgtc atctcgttga
     3301 tcgcgacgtc gaccttcgac tgcgcgtcga cgaacagcgc gttgatccgg tagccctcga
     3361 cgccgccttt ctcgttgaag tagtcgacga tcgccttggt gccgagctgc gccggctccg
     3421 agccgccgcc ggcgaacggg ccggtgacgt cgtagatgaa gccgatcttg atttccttct
     3481 gctgcgcttg cgacagggcc gcgaacgcgc acagcgccat cgcagccaga atccgggtat
     3541 tcctcatggg acgcctcctc tgggcgcaag tctatccgaa ggcttcataa ttggaacact
     3601 gaggaggcag ccgacatgca ttcagccctg atagcagtct tgtgcgcgct gctcgcgctc
     3661 ctgccgggaa cggccgcggc ggcttatccg gagcgcgtga tcacgctgct cgtcgcctat
     3721 ccgcccggcg gcggcaccga cgtcgtcgcg cgcgcgatcg cgccgttcat cgagaaatac
     3781 ctgggtggcg gcgccaagct gctgatcgtg aaccgctcgg gcgccggagg cgaagtgggc
     3841 atgggcgcgc tcgccaacgc accggcggac ggctacacca tcggcttcgt caacacgccg
     3901 ccgctcatga cgatcccgat cgagcgcgcg gcgatcttcg gcggcccgca gcgctttgag
     3961 ctgctgggca acatcatcga cgacccctgc aacttcgccg tgcacgccga cacgccggtg
     4021 aagaacctgc agcagctcgc cgcctgggcg cgcgccaatc ccggcaagct gacggtcggg
     4081 acgacgggcg taggctccga cgaccacctc ctcatgctca tgttcgagcg cgcggcgcgc
     4141 gtgaagctca cgcatgtgcc cttcaagggc tcctccgagg tgcgcgccgc gatcatgggc
     4201 aagcagatcg acgtcgcggc gatgaacgtc ggcgaggcgc tgcaatcgat ccgcggcggc
     4261 gcgccaatgc gtaacctcgg ccagttcggc ccggcgcgct cgaacctcgc gccggacctg
     4321 cccacgggcc gcgagcaggg ctttcccttc gagatggtcg cgctgcgcgg gatggcggcg
     4381 ccgaagggca tgccggcgga catcctcgag cgcctcgtga aggcggtggc acaggctgcg
     4441 gccgaccccg agttccaggc ccaggcggtc aaggtcttcg cgccgctgcg ctacctcgcg
     4501 ccggcacaat tcgatgccga gatccgcgcg ggcgacgccc agttcaggca gctgtggagg
     4561 gagctgccct ggacggacaa gtaggtttgc ccccctgaca aggggggagc gagcttgcga
     4621 gcggggggtt caggaacgca gcactttccc cgccgcggcg ccggtgagcg cgcccttttc
     4681 ccacgtcagc tcgccgttca cgatggtgta ctcgatgcct cgcgagcgca tcaccatgcg
     4741 cttggcgccg cccggcaggt cgtaaagccg ctcgggccgg ccgccggagc cgacggtcgc
     4801 cggatcgaag atcgcgatgt cggccgcgag gcccggcttc aggcgcccgc ggtcgcggat
     4861 gccgaagaaa tccgccgggt ccgacgtcat gcgccgcacc gcttcctcga ttgtgaggac
     4921 ctttctctcc ctcacccagg tgccgagcac gtaggtcgga tagcccgagt cgcacagcat
     4981 gtcgaggtgc gcgccgccgt cgcccaggcc gagcagcacc gagcgatcgt tgaggatctc
     5041 cgccatgcgg tcgacgcggt tgttgtacga ctggatggtg aattcatttt ccagatcatc
     5101 ttgcaaggta atatccagaa gggcgtcgac cccgtccttg ccctgctccg cggcgatttc
     5161 cgcgacggtt tttccttccc atttcttcag ctctggcgag cggacttcat gcagcgtgat
     5221 gcgcgcccag ttgccgaatg aagcggggcg cttcagatcc tcccgaaatt gttttctgaa
     5281 atcaggatcc cggtacacgg cggcttgcgc ggccttcgac ttgtcctcga aggcgcgctt
     5341 ccacgacggg aacccggcga agccgaaggg gttgcgcatg ttgacctcgc gcgtcagcgg
     5401 cagcggcgag gtctgcggcc gcgcgccctt cgcgatcagg tgtcgcacct tgcgcagcgt
     5461 ctcgcgcgcg gcctcgggca ggtcgtcgcg gtcgaacatg gcgatgaagg tgaccgggcg
     5521 gcggctctcc tccagcatga agccgagcag ctcgacctcc ggctcctcca gcaccgcgat
     5581 ctggcgcgtc atcgcgtatt cgatcgagcc cttgccgcgg tcgcgcagca cgttgcagta
     5641 ggccttgatt tcggcccggc tggcgttgcg gcaggcgagc ggccgcgcgc cgtagcccat
     5701 gtgctggttg agcagcgtgc tcgagaagcc gaaggctccg gcatccacgg cttccccgag
     5761 gaggaaactt atttttgaaa gctcggattc agtagcgccg cgctccatcg aggcttcgcc
     5821 catcacgtag tgcctgaacg gcgtgagcgg cgcgaggaac gccaggttca gcgacggctt
     5881 gcggcgcgcc gccgcatcca tgtactgcgg aaagctttcc cagtcccacg tgatcccggc
     5941 atccagcacc tcgaacggga tcgcctccac gttcaccagg tcgcgcatcg cgatctcccg
     6001 cgcctcgggc cggcacggcg cgatgccgac gccgcaattg cccatcacca ccgacgccac
     6061 cccgtgccac gacgaaggcg tcaccgcgcc gtcccagcag atctgcgcgt cgtaatgcgt
     6121 gtgggggtcg atgaaccccg gcgcaacgac caggccgctc gcatcgatgg ttttgcggga
     6181 agcacccgaa atcgacccga tctctactat cctcccgccc gcaaccgcca cgtccgcgcg
     6241 ccttgcgggc gcccctgtgc cgtcgatgag gctgccgttc ctaattagca gatcgcaggc
     6301 catgattcgg cagagtctac tctggtggat cagcgctttc gaccagaagc aggcactcgg
     6361 ggcgtagact gacgactcga gggggttcga ttgaatacga ttcgcaggac cgtggttttg
     6421 gcgctcgcgg cgcttgccct attgcccagg atcggtcgcg gccagcgtgc tctgcgccgt
     6481 attgcgtggt tcggggtagg gctgcccgaa acggtgttgc cgtacctggg tgcgctgcag
     6541 gccggtttgc gcgactcagg ctgggaggag ggccgcaacc tgacgatcga ccgttacagc
     6601 tccacgcgcg cgcccgagga cttcgagcgc atcgtgcgcg agatcgtcga cgcgaagccg
     6661 gaagttgtgg tgacgcagga attcgcgacg ctcgccatgc tgcgtttcgc ggcgagcgtt
     6721 ccagtggtgt ttggctttag cggcgacccg gtcgaggcga agctcgtgaa gagcctcgcc
     6781 cgccccggca ccaatttcac gggcataagc tacctcgcga gcgagctcgt cggcaagcga
     6841 atcgagttcc tgaaggaagc gctgcccggc atccggcgca tcgggatcct cgcgcggccg
     6901 cagcacccgg gcgagcacgg cgaacgtacg gcctcggagg acgcagcgcg caaattgggc
     6961 ctcgctgtag cctatttccc gatccgggac cccgccgaga tcgatcaggc gtttgtcgcg
     7021 atgcgcgatg caaaatgtga tgcgctggtg gtgtttcccg actttacgat gtttacgaac
     7081 cgcgagcggc tcgcccggct cgccgccgcg gcgcggctgc ccgccgtctc ggggtggtcg
     7141 tcgtttgccg atagcggcct gctgttcaat tacggtccaa acctgcgcga catttaccgg
     7201 agtctggctc ccttcgtgga ccgcatcctg cgtggagcga aacccgagga gcttcccgtg
     7261 gagctcccgc gcagcatcga gctcgtcgtc aacctgcgca cggcgcgcga tctcggcata
     7321 aaagtcccgc agtcgatact cgtgcgcgcc gaccgggtga tcgagtgacg aagcgacggg
     7381 ctttcctcgc cgtgatcgga tgcagtgcgc tcgccgcgtc gctcaagtcg ttcgcgcagc
     7441 aaccatcggg caaggggcac cgcattggat tcctgggctc gggttcggcc gtcgcggtgt
     7501 cgaagccgct cgaggagttt cgggcggggc tgcgcgacct cggctacgtc gagggcagga
     7561 acattgccat cgaataccgc tggggggatg ggaagttcga gcggctgccg ggacttgccg
     7621 ccgagctgat ccgcctcaag attgacctga tcgccgtctg gggcactccc gcggcgctcg
     7681 ccgccaagcg ggcgacctcg accctg
//
LOCUS       DASPXW010000061         2087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_146735, whole genome shotgun sequence.
ACCESSION   DASPXW010000061 DASPXW010000000
VERSION     DASPXW010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146735"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VEL04_01465"
     CDS             complement(<1..367)
                     /locus_tag="VEL04_01465"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HYT47064.1"
                     /translation="MNKKLMALAVAGAFGVPAAAMAQVEIYGRANVFMDSYQATGATA
                     ANSDFKSRTRVNDQGSRLGVRGREDLGRGLRAVYQIEAGVNIDTGSQSTSTGSIGASN
                     GASNSATGFLASRDSFVGVE"
     gene            complement(569..1363)
                     /gene="trpC"
                     /locus_tag="VEL04_01470"
     CDS             complement(569..1363)
                     /gene="trpC"
                     /locus_tag="VEL04_01470"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HYT47065.1"
                     /translation="MADILGRILAVKVEEIAQAKQVRSEAALRREAEAAERPRDFVGA
                     LRAKISAGRAAVIAEIKKASPSKGLLREHFDPAGIARSYARHGAACLSVLTDEPFFQG
                     RAEHLGEARAAGGLPALRKDFLIDPYQVYESRALGADCILLIVAALAPPQMRELEAAA
                     ASLGMAVLVEAHDRAELELALELATPLIGINNRDLRSFETRLDTTLSLSREVPASKIV
                     VAESGILAPEHVAQLRQAGVNAFLTGEALMRAEDPGMELERLFLTA"
     gene            complement(1356..>2087)
                     /gene="trpD"
                     /locus_tag="VEL04_01475"
     CDS             complement(1356..>2087)
                     /gene="trpD"
                     /locus_tag="VEL04_01475"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HYT47066.1"
                     /translation="VCAAAGGRVAKHGNRAVSSQSGSADVLEALGANINLKPEQVSRC
                     IDEVGVGFMFAPAHHAAMKHAAPVRRELGVRTIFNILGPLTNPAGAPTQVMGVFHPDL
                     VGIQIRVLQRLGARRAMTVYGLEGLDEISVSGQTMIGELKDGRIHEYLVHPEKFGLPV
                     HDPRALRVATVEESRNVVVAALENKAGPARDIVGLNAGAAIYVAGKAGSFEDGVEKAF
                     GTIKSGAARAKLDQFVKLTQQLARG"
BASE COUNT          352 a          707 c          639 g          389 t
ORIGIN      
        1 cttccacgcc gacaaaggag tcgcgcgacg ccagaaagcc cgtcgctgaa ttagaagcgc
       61 cattactagc accaatagac cccgtcgacg tcgactggct gcccgtatcg atgttcacgc
      121 cggcctcgat ctggtaaaca gccctgagac cgcggcccag atcttccctg ccgcgaacgc
      181 cgaggcgcga gccctggtcg ttcacgcgag tgcgcgactt gaaatcgctg ttggccgcgg
      241 tcgcaccggt cgcctggtag ctatccatga agacattggc gcgtccgtag atctccactt
      301 gcgccatggc cgccgcagga acgccaaacg caccagcaac tgccagtgcc atcagtttct
      361 tgttcatcga gtatttctcc tcttcaattt gattcggggg cagccgaccg cgccgcccgc
      421 tacaacccag tttgagggtt gttgcaattc tgctaatcga agccggcaat ctcaatagga
      481 aacgtgaatt tttgccgctt ctggaagccc gattgttgca tttttacaac accggttacc
      541 gccttccttt aagggtggtt gtttaaaatt aagcagttag aaataggcgt tccagttcca
      601 tcccaggatc ttcggcgcgc atcaaggctt ccccggtcag aaaagcgttg actccagcct
      661 gtcgcaactg cgcgacatgc tcgggggcga gaatcccgct ttcggccacc acgattttcg
      721 acgcaggaac ttcccgggac aagctgaggg tcgtatcgag ccttgtctcg aagctgcgaa
      781 ggtcgcggtt gttgatgccg atgagcggag tggcgagctc aagagcgagc tcgagctcgg
      841 cgcgatcgtg cgcttcgacg agcaccgcca tgccgagcga ggcggcggcc gcctcgagct
      901 cccgcatctg cggcggtgcg agcgcagcga cgatgagcag gatgcaatcg gcgccgaggg
      961 ctcgagattc gtacacctgg tacggatcga tcagaaaatc cttgcgcagc gccggcagcc
     1021 cgccggccgc gcgtgcttcg cccaagtgct cggcccggcc ctggaagaac ggctcgtcgg
     1081 tgagcaccga caggcaggct gcgccgtgcc gcgcgtacga ccgggcgatc ccggccggat
     1141 cgaagtgctc acgcagcaat cctttgctcg ggctcgcctt cttgatctcg gcgatcaccg
     1201 ccgcccgacc ggccgagatt ttcgcgcgca gggcaccgac gaagtcgcgc ggccgctccg
     1261 ccgcctccgc ctcgcgccgc agcgcggcct cgctgcgaac ctgcttcgcc tgcgcgattt
     1321 cctctacctt gacggcgagg attctcccca ggatgtcagc cacgagcgag ctgctgggtg
     1381 agcttgacga actggtcgag cttggcgcgg gccgctccgc tcttgatggt cccgaatgcc
     1441 ttttccactc cgtcctcgaa gcttccggcc ttcccggcga cgtagatggc cgcgcccgcg
     1501 ttgagcccca cgatgtcgcg cgccggtccg gccttgtttt cgagcgcggc gacgaccacg
     1561 ttgcgcgact cctccaccgt cgccacgcgc agcgccctgg gatcgtgcac cggcaggccg
     1621 aatttttccg ggtgaacgag gtactcgtga atcctgccgt ccttcaactc tccgatcatg
     1681 gtctggccgc tcacggagat ctcgtccagc ccctccaggc cgtagacggt catcgcgcgg
     1741 cgcgcaccga gccgctgcag gacccggatc tggattccga ccagatcggg gtgaaacacg
     1801 cccatcacct gggtcggagc gcccgccgga ttggtgagcg gtccgaggat gttgaaaatg
     1861 gtgcgcacgc ccagctcgcg ccgcaccggc gcggcgtgct tcatcgccgc gtggtgcgcc
     1921 ggcgcgaaca tgaagccgac gccgacctcg tcgatgcagc ggctcacttg ctcgggcttg
     1981 aggttgatgt tcgctccgag cgcctccagc acgtcggcgc tgcccgactg ggaagacacc
     2041 gcgcggttgc cgtgcttggc gacgcgcccc cccgctgcgg cgcaaac
//
LOCUS       DASPXW010000062         3729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1156469, whole genome shotgun sequence.
ACCESSION   DASPXW010000062 DASPXW010000000
VERSION     DASPXW010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156469"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="VEL04_01480"
     CDS             complement(<1..1045)
                     /locus_tag="VEL04_01480"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYT47067.1"
                     /translation="MRERLCRWLRAGPHELPALAWAFAYFFLLLCSYYILRPVRDALA
                     VDAGTLTLQRLFSVTFVVMLALLPLFGWLCARLPRARLLPVVYGFFALNLVAFSFSLN
                     SSIFFVWLSVFNLFAVSVFWSFMSDLFDSAQAARLYGSIAAGGSCGAIAGPAIAASAV
                     GWMGVAGLMLVSAAVLAASIVCIALLARWASAHPRSGDAPPEAPIGGSILAGARAALT
                     SPFLAAICGYLLCYTALSTALYFQQIDIVKQAVPDGEERTRLFAVIDLAVNALTLIIQ
                     IFAFSRLTALLGPSWMLALMPLVSAAGFLWLGAAPTLAALVAFGITRRVGEFAISKPV
                     REALFTLVPREERY"
     gene            complement(1052..2029)
                     /locus_tag="VEL04_01485"
     CDS             complement(1052..2029)
                     /locus_tag="VEL04_01485"
                     /inference="COORDINATES: protein motif:HMM:NF033920.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA2778 family cysteine peptidase"
                     /protein_id="HYT47068.1"
                     /translation="MRGRKARSSARVAAGVFILACAALVSGCASFLPQTAALSEGLPS
                     GAAERVELTAVPFFPQSEYQCGPAALATALAAAGVSVTPEDLVPEVYLPERKGSLQIE
                     MLAAARRHGLVSYRLAPSFADLLREISAGTPVIVLQNLGLFDGWHYAVAIGYNYERGG
                     LILRSGTNQRQVLPFAMHELAWKRSGYWAMVAVPPDRIPASAEESRWLASIAALERAG
                     DAKSARIAYSTFLKRWPDNTNAAIGLANAHHALGELDAAEHVLREAMRREPESVIALN
                     NLAQTLSDLGRNEEALAVIERAAAAGGPFAEAVQQTRQTILEKISEKAR"
     gene            complement(2035..2397)
                     /locus_tag="VEL04_01490"
     CDS             complement(2035..2397)
                     /locus_tag="VEL04_01490"
                     /inference="COORDINATES: protein motif:HMM:NF033919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HYT47069.1"
                     /translation="MKSVWARTICRLLVVLMVWTPYQFAQAGMIGTDQVVSAASQADR
                     AALLNFFSRSDVASRLQALGIDSASARDRVATLTDQEVQSLAAQIDSMPAGADSALGA
                     ILIIALLAVAVWYFWKRM"
     gene            2487..>3729
                     /locus_tag="VEL04_01495"
     CDS             2487..>3729
                     /locus_tag="VEL04_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HYT47070.1"
                     /translation="MADFQRLERELRSAVAGEVRFDEGARAVYASDASNYRQVPIGVV
                     LPRSTDDIVAAVALCRAHGAPLLARGGGTSQCGQGVNVAVVIDCSKYLHQVLGVDAQA
                     GLARVEPGAACDALRDAAEQHGLTFAPDPATHSRCTLGGMIGNNSCGPHSLMAGKTVE
                     NIERLEVLTYDGARFWCGATAPQNLQKIIFEGGRRGKIYSDLKAIADKYAALVRSGFP
                     KIKRRVSGYNLDQLLPENGFNLARALVGSEGTCALTLQAEARLVKSPRERVLVVLGFP
                     DIFAAGDAVPRLLESGPIACEGLDERIIGGLRERRLALEEIALLPPGNGWLMLEFGGD
                     SREEVLSRAKQLPGLLIEDRALMARLWSIRETGASATALSLGGSGRDQLVGWEDAAVD
                     PMRIGDYLREFRALIARFGYRT"
BASE COUNT          650 a         1242 c         1316 g          521 t
ORIGIN      
        1 tgtagcgctc ctcgcgcggc accagcgtga aaagcgcttc gcgcaccggc ttcgagatgg
       61 cgaattctcc gacccgccgc gtgatgccga aggcgaccag cgcggcgagc gtcggcgccg
      121 ccccgagcca gaggaatccc gcggcggaga cgagcggcat cagcgcgagc atccaggacg
      181 ggccgagcag cgcggtcagg cgcgagaacg cgaatatctg gatgatcagg gtcagcgcgt
      241 tgaccgccag gtcgatgacg gcgaagaggc gggtgcgctc ctcgccgtca ggcaccgcct
      301 gcttcactat gtcgatctgc tggaagtaga gcgccgtcga aagcgcggtg tagcacagca
      361 aatagccgca gatggcggcg aggaacggcg aggtgagcgc cgcgcgtgct ccggcgagga
      421 tcgagccgcc gatcggcgcc tccggcggcg cgtcgccgct gcgcgggtgg gcgctcgccc
      481 agcgcgcaag cagcgcaatg cagacgatgc tcgcggcgag gacggctgcg gagaccagca
      541 tcaggccggc aacccccatc caccccacag cgcttgccgc gatcgctggc ccggcaatcg
      601 cgccgcagct gccgccagcg gcgatgctgc cgtacaggcg tgccgcctgg gcgctgtcga
      661 aaagatccga catgaagctc cagaacaccg agaccgcgaa cagattgaac acgctcagcc
      721 agacgaaaaa tatcgaagaa ttcagtgaaa aggagaaggc gacgaggttc agggcgaaaa
      781 aaccgtaaac caccggcagc agccgcgcgc gcggcagccg cgcgcaaagc cagccgaaca
      841 gcggcagcag cgcgagcatg acgacgaagg tcacgctgaa aagccgctgc aacgtcagcg
      901 tgccggcatc gaccgcgagc gcatcgcgca ccggccgcag gatgtagtag ctgcaaagca
      961 gcaggaagaa gtaggcgaac gcccacgcga gcgccggcag ctcgtgcggc ccggcgcgca
     1021 gccagcggca gaggcgctcg cgcattgccg gctagcgcgc tttctcggag atcttctcga
     1081 ggatcgtctg ccgtgtctgc tgcacggcct cggcaaacgg cccgcccgcg gccgccgcac
     1141 gctcgatcac cgcgagggct tcctcgttgc gcccgagatc cgagagcgtc tgggcgaggt
     1201 tgttgagggc gatgaccgac tccggctcgc ggcgcattgc ttcgcgcagc acgtgttcgg
     1261 ctgcgtcgag ctcgccgagc gcatggtgcg cgttggcgag cccgatcgcg gcgttcgtgt
     1321 tgtccggcca gcgcttgagg aaggtcgagt aggcgatgcg cgcgctcttc gcgtcgccgg
     1381 cgcgctcgag cgcggcgatc gaggcgagcc agcgcgactc ctccgcgctc gccgggatgc
     1441 ggtcgggcgg caccgcgacc atcgcccagt agccgctgcg cttccaggcg agctcgtgca
     1501 tcgcgaaggg caagacctgc cgctggttcg tgcccgagcg caggatcagc ccgccgcgct
     1561 catagttgta gccgatcgcg accgcgtagt gccagccgtc gaacaggccc aggttctgca
     1621 gcacgatcac cggcgtgccg gccgagatct cgcgcagcag gtcggcgaag ctcggcgcga
     1681 gccgatagga cacgaggccg tgccgccgtg ccgccgccag catctcgatc tgcaggctgc
     1741 ctttgcgctc gggcaggtaa acctcaggca cgaggtcctc gggcgtcacg cttacgccgg
     1801 cggcggcgag cgcggtggcg agcgcggcag gtccgcactg gtactcgctt tgcgggaaga
     1861 agggcaccgc ggtaagctcg acgcgctcgg cggcgccgct cggcaagcct tcgctgagcg
     1921 ccgcggtctg cggcaggaag ctcgcgcagc ccgaaaccag ggccgcgcaa gccaaaatga
     1981 aaacgcccgc ggcgacgcgg gcgctcgagc gggccttgcg gccgcgcacg gatatcacat
     2041 gcgcttccag aagtaccaga ccgctacggc gagcagcgcg atgatcagga tcgcgcccag
     2101 cgcggaatcg gcgccggcgg gcatggagtc gatctgtgcg gcgagcgact gcacttcctg
     2161 gtcggtgagg gtggcgacgc gatccctggc gctggcggag tcgatgccga gcgcctgcag
     2221 ccggctcgcg acatcgctgc ggctgaagaa gttgagcagc gccgcgcgat cggcctgcga
     2281 cgcggcagac acgacctgat cggtgccgat cattccggcc tgcgcgaact ggtacggtgt
     2341 ccagaccatc agcactacca gcaagcggca gatcgttctt gcccagacgg atttcatgtg
     2401 ccctcctaag ggagtgagag ggagtgacag cctagtattc gaccggtgcg aagtcaaaga
     2461 attcccgtat gccagaatca ctgcgaatgg cagattttca acgacttgag cgggagctgc
     2521 gcagcgccgt cgcaggcgag gtccggttcg acgaaggcgc gcgcgcggta tacgcatcgg
     2581 atgcatcgaa ttaccgccag gtgccgatcg gcgtggtgct gccgcgcagc accgacgaca
     2641 tcgtcgctgc cgtagcgctg tgccgcgcgc acggcgcgcc cctgcttgca cgcggcggcg
     2701 gcacttcgca gtgcggccag ggcgtgaatg tcgccgtggt gatcgactgc tcgaaatacc
     2761 tgcaccaggt tctcggcgtc gacgcgcagg caggccttgc gcgcgtcgag cccggcgcgg
     2821 cctgcgacgc gttgcgcgac gcggccgagc agcacggcct gactttcgcc cccgacccgg
     2881 cgacgcactc gcgctgcacg ctcggcggca tgatcggcaa caactcctgc ggcccgcatt
     2941 cgctgatggc gggcaagaca gtcgagaaca tcgagcgcct cgaagtcctg acctacgacg
     3001 gcgcgcgctt ctggtgcgga gcgaccgcgc cgcagaatct tcaaaagata attttcgaag
     3061 gaggacgcag ggggaagatc tactccgacc tgaaggcgat tgcggacaag tatgccgcgc
     3121 tcgtgcgcag cggctttccg aaaatcaagc ggcgcgtctc gggctacaac ctcgaccagc
     3181 tgctgcccga aaacggcttc aacctcgcgc gcgcgctggt cggcagcgag ggcacctgcg
     3241 cgctgacgct ccaggccgaa gcgcggctgg tgaaaagccc gcgcgagcgg gtgctggtgg
     3301 tgctcggctt tcccgacatt ttcgccgccg gcgacgcggt gccgcggctc ctcgagagcg
     3361 ggccgatcgc gtgcgaaggg ctcgacgagc gcatcatcgg cggtctgcgc gagcggcggc
     3421 tcgcgctcga ggagatcgcg ctgctgccgc cgggcaacgg ctggctgatg ctcgagttcg
     3481 gcggcgactc gagggaagaa gttctttccc gggcgaagca gctgcccgga cttctgatcg
     3541 aggaccgcgc gctcatggcg cgcctgtggt cgatccgcga aaccggcgcc tcggcgactg
     3601 cgctcagtct cggcggcagc gggcgcgatc agctggtcgg ctgggaagac gccgccgtgg
     3661 acccgatgcg catcggcgac tacctgcgcg aattccgcgc gctcatcgcg cgcttcggct
     3721 accgcacct
//
LOCUS       DASPXW010000063         3092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1787203, whole genome shotgun sequence.
ACCESSION   DASPXW010000063 DASPXW010000000
VERSION     DASPXW010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1787203"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="VEL04_01500"
     CDS             <1..611
                     /locus_tag="VEL04_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47071.1"
                     /translation="LMIGLGGGSLAKFFFKRFRKTRVRVVELDARVVAAARAHFGLPP
                     DDARLSVEIGDGAEALSPECCDLLVVDAFHDEQHVPKLASAEFYAAAFLALAEPGAMV
                     VNFMDDDPRFDQTLQRLEAAFGGAVLAMPALYDPNIVAFAFKGLAPAFEWQALRRRAE
                     RLEARFGLPFTRYVSRLRAMNRSTARELLISLSQKFSFPGAR"
     gene            625..1326
                     /locus_tag="VEL04_01505"
     CDS             625..1326
                     /locus_tag="VEL04_01505"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HYT47072.1"
                     /translation="MAFLGALVFGITGFGAALVTIPLATHLVPLKFALALFAISDLAM
                     ALRVGFENPRNAVRAEWLRLMPMIVAGTALGVTLLVNLPREAGMLLLGVFVAAYAVYA
                     LMRHEHLKPVCVAWAWLAGFAGGITSTLFGAGGPPYAIYLSQRGLSKEQFRATLGFAT
                     MTSISLRVLAFLLTGLLLDARVWLTAVAVVPAALAGIAFARKIFLRISRDVLMRVIAL
                     LLLASGGSLVWRALG"
     gene            complement(1356..2120)
                     /locus_tag="VEL04_01510"
     CDS             complement(1356..2120)
                     /locus_tag="VEL04_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HYT47073.1"
                     /translation="MRRAFLLVLLALPLLTGLAAAAPPDRQLVEDLVAANRILAMEGV
                     LDGYGHVSVRHDQDPSRYLMSRYIAPALVTAADIVEYDLDSNALEPKGAPSYTERFIH
                     GEIYKMRADVKAIVHNHSPALIPFGISGVPLRPVFHMSAFIGQGVPVFEIRESGGMTD
                     MLVRSAPLGQALARMLGNHPAALMRGHGAVVVGPTLPLAVGRSVYLETNARLQAQAAA
                     LGSSLTFLDAEEVRKVEARQDYKRAWELWKRKAMTK"
     gene            complement(2120..>3092)
                     /locus_tag="VEL04_01515"
     CDS             complement(2120..>3092)
                     /locus_tag="VEL04_01515"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYT47074.1"
                     /translation="KKITAKHILVATGSWPVLPQIPGREHAISSNEAFHLERLPARAL
                     VVGGGYIALEFASIFHGLGVKTTLSYRGKRLLRGFDAELGSRIAEEMTAKGIAICFGC
                     EPAAMRKRADGSLQIEFTDGRLQEAELVMFATGRRPNAAKLGLESAGVKLAADGAVIV
                     DRFSQTTVDSIHAIGDVTNRLNLTPVATAEAMWLARTLWREPTPVDHDNVPTAVFANP
                     NLAAVGLSEERARERHGEVDIYKASFRALKLTLAESKERTFMKLVVDRASQRVVGVHM
                     IGADAGEVIQGMAIAVKLGATKAQFDATLGIHPTAAEEFVTMREKAA"
BASE COUNT          419 a         1055 c         1046 g          572 t
ORIGIN      
        1 cgctgatgat cggtctcggc ggcggttcgc tggcgaagtt ttttttcaag cgtttcagaa
       61 aaaccagagt gcgcgtggtc gagctcgatg cgcgcgtcgt agccgccgcc cgcgcgcatt
      121 tcgggctgcc gcccgatgac gcgcgcctct cggtcgagat cggcgacggc gccgaggccc
      181 tgtcgcccga atgctgcgac ctgctggtgg tcgatgcgtt tcacgacgag cagcacgtgc
      241 cgaagctcgc gagcgccgag ttctacgccg ccgcgttcct cgcgcttgcc gagcccggcg
      301 cgatggtggt caatttcatg gacgacgacc cgaggttcga ccagaccctg cagcgcctcg
      361 aggccgcatt cggcggcgcg gtgctcgcca tgcccgcgct ctacgatccg aacatcgtcg
      421 cgtttgcttt caagggcctt gcgccggcct tcgaatggca agcgctgcgc cgccgcgccg
      481 agcggctcga agcgcgcttc ggcctgccct tcacccgtta cgtttcgcgc ttgcgggcca
      541 tgaatcgctc tactgcgcgc gagctgctta tttccctctc acagaaattc tcattccctg
      601 gggcgaggtg acgctgcttg cggcattgcc ttccttggcg cgctggtctt cggcatcacc
      661 ggcttcggcg cggcgctggt cacgatcccg ctcgccacgc acctcgtgcc gctcaagttc
      721 gcgctggcgc tgttcgcgat ctcggacctg gcgatggccc tgcgcgtggg tttcgagaac
      781 ccgaggaacg cggtgcgcgc cgaatggctg cgcctcatgc cgatgatcgt cgccggcact
      841 gcgctcggcg tcacgctgct cgtgaatctg ccgcgcgagg cggggatgct gctgctcggg
      901 gtttttgtgg ccgcctatgc cgtctatgcg ctgatgcgcc acgagcacct gaagcccgtt
      961 tgcgtcgcct gggcgtggct cgccggattc gccggcggta tcaccagcac gctgttcggc
     1021 gccggcggcc cgccatacgc gatctacctt tcgcagcgcg ggctctcgaa ggagcagttc
     1081 cgcgccacgc taggcttcgc cacgatgacc agcatctccc tcagagtgct cgctttcctt
     1141 ctcaccggat tgctgctcga tgcgcgggtc tggctgaccg ccgtcgcggt ggtgccggcc
     1201 gcgctcgcag gcatagcctt tgccagaaag atttttctga ggatctcgag ggacgtgctg
     1261 atgcgggtga ttgcgctgct gctgctcgcc tcaggcggct cgctggtgtg gcgtgcgctc
     1321 ggctgaaggc agccgccggc ccgggaccgg tgctcttatt tcgtcatcgc cttgcgcttc
     1381 cagagctccc aggcgcgctt gtagtcctgc cgcgcctcga ccttgcgcac ttcctcggcg
     1441 tcgaggaagg tgagcgagct gcccagcgcc gcagcctggg cctggagcct ggcgttggtc
     1501 tcgagataaa cgctgcgccc gacggcgagc ggcaacgtcg ggcccaccac caccgcgccg
     1561 tgcccgcgca tcagcgccgc gggatgattg ccgagcatgc gagcgagcgc ctgcccgagc
     1621 ggggcgcttc gcaccagcat gtcggtcatg ccgcccgact cgcggatctc gaacaccggc
     1681 accccctgcc cgatgaaggc gctcatgtgg aacaccggcc tgaggggtac gccgctgatg
     1741 ccgaagggga tcagcgccgg cgaatggttg tggacgatcg ccttcacgtc ggctcgcatc
     1801 ttgtagatct cgccgtggat gaagcgctcc gtataggagg gcgcgccttt cggctcgagc
     1861 gcgttgctgt ccagatcgta ttcgacgatg tccgccgcgg tcaccagcgc gggcgcaatg
     1921 tagcgtgaca tcaggtagcg gctggggtcc tggtcgtggc gcacgctgac gtgcccatag
     1981 ccgtcgagca cgccctccat cgcgaggatg cggttcgccg ccaccaggtc ctccaccagc
     2041 tgtcgatccg gcggtgcggc cgccgcgagg ccggtgagca ggggcagcgc cagcaggacg
     2101 agaagaaacg cccgtctcat caggcggcct tctcccgcat ggtgacgaac tcctcggcgg
     2161 cggtcggatg gatgccgaga gtggcgtcga actgcgcctt ggtggcgccg agcttcaccg
     2221 cgattgccat gccctggatc acctcgccgg cgtcggcccc gatcatgtgc acgccgacca
     2281 cgcgctggct cgcccggtcg accaccagct tcatgaaggt gcgctccttg ctctcggcga
     2341 gcgtcagctt gagggcgcgg aacgacgcct tgtagatgtc gacctcgccg tggcgctcgc
     2401 gcgcgcgctc ctcgctcaag cccaccgcgg cgaggttcgg attggcgaac accgcggtcg
     2461 gcacgttatc gtggtcgacg ggcgtgggct cgcgccacag cgttcttgcg agccacatcg
     2521 cttccgcggt ggcgaccggc gtgaggttga ggcggttggt cacgtcgccg atggcgtgga
     2581 tcgagtcgac ggtagtttgc gagaagcgat cgacgatcac cgcgccgtcg gcggcgagct
     2641 tcacgcccgc gctctcgagc ccgagcttgg cggcgttcgg tcgccgcccg gtggcgaaca
     2701 tcaccagctc tgcctcctgc agcctgccgt cggtgaattc gatctgcagg ctgccgtcgg
     2761 cgcgcttgcg catcgcggcc ggctcgcagc cgaagcagat ggcgatgcct ttcgccgtca
     2821 tctcctcggc gatgcggctg ccgagctcgg cgtcgaagcc gcgcagcagg cgcttgccgc
     2881 gataggagag cgtggtcttc acgcccaggc cgtggaagat cgacgcgaac tcgagcgcga
     2941 tgtagccgcc gccgacgacc agcgcgcgcg ccggcagccg ctcgagatgg aaggcctcgt
     3001 tcgagctgat ggcgtgctcg cgccccggga tctgcggcag caccggccac gagccggtgg
     3061 cgacgagaat atgcttcgca gtgatcttct tt
//
LOCUS       DASPXW010000064         2114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_273460, whole genome shotgun sequence.
ACCESSION   DASPXW010000064 DASPXW010000000
VERSION     DASPXW010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273460"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VEL04_01520"
     CDS             complement(<1..188)
                     /locus_tag="VEL04_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HYT47075.1"
                     /translation="MIVYLHGFNSSPQSHKAQLLGRYLEARGLGAEYACPALPPLASD
                     AVGEIEKLIKQNAGVGFVG"
     gene            complement(185..1549)
                     /gene="mpl"
                     /locus_tag="VEL04_01525"
     CDS             complement(185..1549)
                     /gene="mpl"
                     /locus_tag="VEL04_01525"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HYT47076.1"
                     /translation="MAAHLHILGICGTFMGGIAAIAKAAGYRITGCDANVYPPMSEQL
                     EGLGIELVKGFGEEQLALRPDLWVIGNVVTRGNPLMEAILDRGERYVSGPQWLAEHVL
                     AGRHVLAVAGTHGKTTVAAMLAWLLERAARNPGFLIGGVPLDFRVSARYTESKYFVIE
                     ADEYDTAFFDKRSKFVHYSSRTAVLNNLEFDHADIFDDLAAIERQFHHFVRTVPSSGL
                     LVVNGADQALERVLTQGCWTPVERFGVPQGWAAGEPDAEGGFEVAWQGRALGRVRWQQ
                     FGAHNRLNALAAIAAARHVGVEPAQAIEALGTFSGVRRRMQVRGLARGVTVYDDFAHH
                     PTAIRVTLEGLRQRIGGARILAVLEPRSNTMKRGVMKEALPASLAQADRVFVYTVGLG
                     WDAQAVFSPLGERARCIDELDRLVAAVAAEARTGDQVLVMSNGGFGGIHDKLLERLRR
                     GVAA"
     gene            1663..>2114
                     /locus_tag="VEL04_01530"
     CDS             1663..>2114
                     /locus_tag="VEL04_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47077.1"
                     /translation="MRARIAAAAARIMAEDGIDDFALAKRKAAKQLGAGDLQALPAND
                     EVELELRAYRALYQANEHPQRISGLRRVALDAMRALERFNPYLTGPILTGTAGPYAQI
                     DLQLFPESTKEVELFLLDRNVAYSTQEARRYSGDRAHAVSVLSLDWQGT"
BASE COUNT          396 a          714 c          667 g          337 t
ORIGIN      
        1 ccgacgaaac caacccctgc attttgcttg atgagttttt cgatttcgcc gaccgcgtcg
       61 gaggcgagcg gcggtagcgc cgggcaggca tattcggccc cgagaccccg tgcctccagg
      121 tagcgcccga gcagctgcgc cttgtgcgat tgcggcgagg agttgaagcc atgcaggtag
      181 acaatcacgc cgccacgccc cgccgcagcc gctcaagcag cttgtcatgg atgccaccga
      241 agccgccgtt ggacatcacg agcacctggt cccccgtgcg agcctcggcg gcaaccgccg
      301 ccacgagccg gtcgagctcg tcgatacagc gcgcgcgttc cccgagcggc gagaacacgg
      361 cctgcgcgtc ccagccaagc ccgacggtat agacaaacac gcggtcagcc tgggcgaggc
      421 tggcgggcag tgcttctttc atgacgccgc gcttcattgt gttggagcgc ggctcgagga
      481 cggcgagaat acgcgcgccg ccgatccgct gccgcaggcc ctcgagggtg acgcggattg
      541 cggtcgggtg gtgcgcgaag tcgtcgtaga cggtgactcc ccgggcgagg ccccgtacct
      601 gcatgcgccg gcgcaccccg gagaaagtgc caagcgcctc gatcgcctgc gccggctcga
      661 ccccgacatg gcgtgccgcg gcgatcgcgg cgagcgcatt cagccggttg tgcgcgccaa
      721 actgctgcca gcgcacccgg ccgagagccc tgccctgcca cgctacttcg aagccgcctt
      781 cggcatccgg ctcgccagcc gcccagcctt gagggacgcc aaagcgctcg actggcgtcc
      841 agcagccttg ggtgagtact cgctccaacg cctgatcggc accattcacc accagcaaac
      901 ccgagctcgg gacagttcgc acaaaatgat ggaattgccg ctctatcgct gcaagatcgt
      961 cgaagatgtc tgcatgatcg aactcaagat tattgaggac agcggttctg gagctgtaat
     1021 gcacaaattt ggagcgttta tcgaaaaagg ccgtgtcgta ctcatcggct tcaataacga
     1081 agtacttact ctcggtgtac ctggctgaaa cccggaagtc gagcggcact ccgccaatga
     1141 ggaagccagg attccgcgcc gcgcgctcaa gcagccaggc gagcatcgcg gccactgtgg
     1201 tcttgccatg tgtacccgcg accgccagca catgccgtcc cgcgagcaca tgctcggcga
     1261 gccactgcgg tcccgaaacg tagcgctcgc cccggtccag gatcgcttcc atcagcgggt
     1321 tgccccgggt caccacgttg ccgatgaccc agagatccgg gcgcagcgcg agctgctcct
     1381 cgccgaaccc cttgacgagc tcaatgccaa ggccttcgag ttgctcgctc atcggcgggt
     1441 agacgttggc atcgcagccc gttatgcgat agccggctgc cttcgcaatc gcagcaatgc
     1501 cacccatgaa ggtgccgcag atcccgagga tgtggaggtg cgccgccatg ggggaagcgc
     1561 cagattctat tagatggatg ggagcgcccc tacgctatga ttgccgcgcc ccatgagccg
     1621 caaagcgagg ccggaatgag ccacaagagc aggcagaccg gcatgcgcgc ccgcatcgct
     1681 gcggcggcgg cgcgtatcat ggctgaggac ggcattgacg atttcgcgct cgccaagcgg
     1741 aaggccgcga agcagcttgg ggcgggtgac ctgcaggcgc ttccggcaaa cgatgaggtc
     1801 gaactggagc tgcgcgcgta ccgtgcgctc taccaggcca acgagcatcc gcagcggatc
     1861 agcggcttgc gccgcgtcgc gctcgacgcc atgcgagcgc tcgagcgctt caacccctac
     1921 ctcaccggcc caatactgac cgggaccgcc ggaccgtacg cgcagatcga cctgcaatta
     1981 ttcccggaga gcacgaaaga ggtggagttg tttctgctcg accgcaacgt cgcctacagc
     2041 acgcaggaag cgcgccgcta ctctggcgac cgcgcccacg ctgtgagcgt gctctcgctc
     2101 gactggcagg gaac
//
LOCUS       DASPXW010000065         5071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1410203, whole genome shotgun sequence.
ACCESSION   DASPXW010000065 DASPXW010000000
VERSION     DASPXW010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5071
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410203"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1071)
                     /gene="guaB"
                     /locus_tag="VEL04_01535"
     CDS             complement(<1..1071)
                     /gene="guaB"
                     /locus_tag="VEL04_01535"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HYT47078.1"
                     /translation="MRVIQKALTFDDVLLLPAHSRVLPREVSLKTRFTRKLELNSPLV
                     SAAMDTVTEARLAIAMAQEGGIGIIHKNFPPQAQAAEVAKVKRFESGVLRDPMTIPPE
                     MTVRQAIALQQQHKISGFPVVKQGKVVGIVTNRDLRFETQLDQPVRAIMTPQRKLITV
                     REGVSREEARTLMHRHRLERVLVIDDQFHLKGLITVKDILKETEHPNACKDAQGKLRV
                     GAAVGVGEGTDERVAALVEAGADVLVVDTAHGHAQGVLDRVKWIKTRFPQVEVVGGNI
                     GTGDGARALVDHGADGVKVGIGPGSICTTRLVAGVGVPQITAIQHVAEALRDAGIPCI
                     ADGGIRYSGDIAKAIAAGANSVM"
     gene            1135..1590
                     /locus_tag="VEL04_01540"
     CDS             1135..1590
                     /locus_tag="VEL04_01540"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HYT47079.1"
                     /translation="MNKILLFVCMMGVAFGGMAQQYKWVDKDGKVQYGDTPPPGVKPS
                     PLRPPPGPSQAPPAAAKKDAEKALSPEAAFRKRQQEAKEKDEKAGKERAEAETRRTNC
                     DGAQVTLRQLQSGVRMSTMNAAGERVFIDDEQRAKETERAQRAVSEWCK"
     gene            complement(1592..1864)
                     /locus_tag="VEL04_01545"
     CDS             complement(1592..1864)
                     /locus_tag="VEL04_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006764044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HYT47080.1"
                     /translation="MRVEVVYALAGREDAVAVVLPSGATVRDAVLASGIARRHPEIDL
                     GSSKLGIFGKTVAPAARVADGDRVEIYRTLAMDPKEARRRRVKRKL"
     gene            complement(1854..2273)
                     /locus_tag="VEL04_01550"
     CDS             complement(1854..2273)
                     /locus_tag="VEL04_01550"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HYT47081.1"
                     /translation="MKRTARSAIVEHAAREMFDLVEDIESYPKFLPWCLGATVHERSA
                     TGTRATLTVGLRGLKQSFTTQNANRAGESIEMRLLRGPFRHFSAAWRFRAAGEHACHV
                     EFSMEYEFASRTLGKLLAPLFDHIADTMVDAFTRRAG"
     gene            2289..2735
                     /gene="smpB"
                     /locus_tag="VEL04_01555"
     CDS             2289..2735
                     /gene="smpB"
                     /locus_tag="VEL04_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HYT47082.1"
                     /translation="MSIVQNRKAFHDYFIEERYEAGLALEGWEVKSVRGGRAQLAEGY
                     VIVRGGAELYLIGAHISPLPTVSTHFTPDPTRSRKLLLHAEEIRRLIGKVEQRGYTLV
                     PLDLHYSKGRIKLEIGLARGKLKHDKRAAERDKQWNREKQRLLRSR"
     gene            complement(2721..>5071)
                     /gene="ppsA"
                     /locus_tag="VEL04_01560"
     CDS             complement(2721..>5071)
                     /gene="ppsA"
                     /locus_tag="VEL04_01560"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HYT47083.1"
                     /translation="DLPRVGGKNASLGEMIGSLTAAGIRVPGGFATTADAFREFLSAS
                     GLDAPIAQCLRKLDPKDVTALAAGGAEIRSWILKAPFPAAFEQEIRSYFQTLEKQTSG
                     DTSYAVRSSATAEDLPDASFAGQQETFLNIRGVDNVLEAIRHVFASLYNDRAISHRAH
                     QGFEQGVALSAAVQRMVRSDLGASGVSFTLDTESGFRDVVFITASYGLGEMLVQGAVN
                     PDEFYVHKPMLERGKPAIVRRRLGSKLQKMVFSQSAAAGRSTRVTEVAEAERNSFSLK
                     DDEVLELARYAAVIERHYGRPMDIEWGKDGADGRIYVLQARPETVRSRKREVEQRFAL
                     KGSSRVLASGRAIGHKIGSGTVRFVPDASQMARVKQGDVLVTDMTDPNWEPVMKLAAA
                     IVTNRGGRTCHAAIIARELGIPAVVGCGDATSRLRDGQAVTVSCADGDTGNVFEGLLP
                     FEVSARERGEMPRIPVKIAMNVGNPQLAFEFAQLPNAGVGLARLEFIINHEIGAHPRA
                     CLEYETLPDELKSKLAAKSRGYADPRTFFREKMVEGVATIAAAFWPKPVIVRLSDFKS
                     NEYKKLIGGERYEPDEENPMLGFRGASRYIAPAFRECFELECAALKKVRDELGLTNVE
                     LMVPFVRTLGEAQQVMSLLEKNGLKRGLNGLRVVMMCELPSNALLAEEFLELFDGFSI
                     GSNDLTQLTLGLDRDSGLVAETFDERDAAVKLMLAMAIRACRKHGKYVGICGQGPSDH
                     PDLARWLMEQGIESLSLNPDTVVSTWMALAENPPPQKKSATA"
BASE COUNT          857 a         1679 c         1693 g          842 t
ORIGIN      
        1 catcacgctg ttggctcctg cggcgatcgc cttggcgatg tccccggagt agcgaatgcc
       61 gccgtcggcg atgcacggga tgccggcgtc gcgcaacgcc tcggccacgt gctggatggc
      121 ggtgatctgc ggcacgccca cgcccgccac gaggcgcgtg gtgcagatcg agcccggccc
      181 gatgccgacc ttgacgccgt cggcgccgtg gtcgaccaac gcgcgcgcgc cgtcgccggt
      241 gccgatattg ccgcccacca cctcgacctg cggaaagcgc gtcttgatcc atttcacccg
      301 gtcgagcacg ccctgcgcgt ggccgtgggc ggtgtcgacc accagcacgt cggcgcccgc
      361 ctcgaccagg gccgcgacgc gctcgtcggt gccttctccg accccgaccg cggcgccgac
      421 gcgcagcttc ccctgcgcgt ccttgcaggc gttcggatgc tcggtttcct tgaggatgtc
      481 cttgacagtg atcaggccct tgaggtggaa ctggtcgtcg atcaccagca cgcgctcgag
      541 gcggtgccgg tgcatcaggg ttctggcctc ctcgcggctc acgccctcgc gcaccgtgat
      601 gagcttgcgc tgcggcgtca tgatggcgcg caccggctgg tcgagctggg tctcgaagcg
      661 caggtcgcgg ttggtgacga tgccgaccac cttgccctgc tttaccaccg gaaagcccga
      721 gatcttgtgc tgctgctgca gcgcgatcgc ctggcgcacc gtcatttccg ggggaatggt
      781 catcgggtcg cgcagcacgc cggactcgaa gcgcttcacc ttggcgactt cggccgcctg
      841 cgcctgcggg ggaaaattct tgtggatgat gccgatgccg ccctcctgcg ccatcgcgat
      901 cgcgagccgc gcctcggtga ccgtgtccat ggcggcagac accagcgggc tgttcagctc
      961 gagcttgcgg gtgaagcggg ttttcaggga gacttcgcgc ggcaggacac gcgagtgggc
     1021 gggaagaagg aggacgtcgt cgaaggtcag cgccttctgg atcacccgca tgatgtccgc
     1081 tcgcaaagcg gcattatact gtcatccatc gatcgctccg gacgttgagt atccatgaac
     1141 aaaattctgc tgttcgtctg catgatggga gtcgccttcg gcggcatggc ccagcaatac
     1201 aagtgggtcg acaaggacgg caaagtccag tacggcgaca cgccgccgcc cggggtcaag
     1261 ccgtcgcctc tcaggccgcc gccgggaccg agccaggcgc cgccagcggc ggcgaagaag
     1321 gacgccgaaa aggcactcag cccggaggcc gcgttccgca agcgccagca ggaggcgaag
     1381 gaaaaagacg agaaggcggg caaagagcgc gccgaggccg agaccaggcg gacaaattgc
     1441 gatggcgcgc aagtcacgct gcgccagctt cagagcggtg tgcgcatgag caccatgaac
     1501 gccgcggggg agcgggtatt catcgacgac gagcagcggg cgaaggagac ggagcgcgcg
     1561 cagcgcgccg tcagcgagtg gtgcaaataa gctagagctt cctcttcacg cgccggcgcc
     1621 gggcctcctt cgggtccatc gccagcgtcc ggtagatctc gacgcggtcg ccgtcggcga
     1681 cgcgcgccgc cggcgccacg gtcttgccga agatcccgag cttgctgctg ccgaggtcga
     1741 tctccgggtg gcgccgcgcg attcccgaag cgagcaccgc gtcgcgcacc gtcgcgccag
     1801 acggcaggac gacggccacg gcgtcctcgc ggccggcaag cgcgtagacg acttcaaccc
     1861 gcacggcgcg tgaaggcgtc gaccatggtg tcggcgatat ggtcgaaaag cggtgcgagc
     1921 agcttgccga gggtgcggct cgcgaactcg tactccatcg agaactcgac gtggcaggca
     1981 tgctctcccg ccgcgcggaa gcgccaggcg gcagaaaaat ggcggaacgg cccgcgcagc
     2041 aggcgcatct cgatcgactc gccggcgcgg ttcgcgttct gcgtggtgaa cgactgcttc
     2101 aagccgcgca agcccacggt gagcgtggcg cgggttcccg tcgcgctgcg ttcgtgaacc
     2161 gtcgcaccga ggcaccaggg caggaatttc gggtacgact cgatgtcttc caccaggtcg
     2221 aacatctccc gggccgcgtg ctcgactatg gcggaacggg cggtgcgctt catgaactag
     2281 aattcttcat gtccatcgtg cagaaccgca aggcgtttca cgattacttc atcgaggagc
     2341 gctacgaggc ggggctggcg ctcgagggct gggaagtcaa gtcggtgcgc ggcgggcgcg
     2401 cgcagctcgc agaaggctac gtcatcgtgc gcggcggcgc cgagctctac ctgatcggcg
     2461 cgcacatcag cccgctgccc acggtgtcga cccatttcac gcccgacccg acccgcagcc
     2521 gcaagctgct gctgcacgcc gaggagatcc gcaggctgat cggcaaggtt gagcagcgcg
     2581 gctacacgct ggtgccgctc gacctgcatt acagcaaagg ccgcatcaag cttgagatcg
     2641 ggctcgcgcg cggcaagctc aagcacgaca agcgcgccgc cgagcgcgac aagcagtgga
     2701 accgcgagaa acagcgcttg ctacgcagtc gctgattttt tttgcggcgg agggttctcg
     2761 gccagcgcca tccaggtcga gaccacggtg tcggggttga gcgagaggct ctcgatgccc
     2821 tgctccatca gccagcgggc gagatcggga tggtctgagg gcccctggcc gcagatgccg
     2881 acgtacttgc cgtgcttgcg gcaggcgcgg atcgccatgg cgagcatcag cttcaccgcc
     2941 gcgtcgcgct cgtcgaaggt ttcggcgacc agcccagaat cgcggtcaag gccgagcgtg
     3001 agctgcgtca ggtcgttgga gccgatcgag aagccgtcga acagctcgag aaactcctcg
     3061 gcgagcagag cgttggacgg cagctcgcac atcatgacga cgcgcaggcc gttcagaccc
     3121 cgcttcaggc cattcttttc cagcagactc atcacttgct gcgcttcgcc gagcgtgcgc
     3181 acgaacggca ccatcagctc gacgttggtg aggccgagct cgtcgcgcac tttcttcagc
     3241 gccgcgcatt ccagctcgaa gcattcgcgg aaggcgggcg cgatgtagcg cgacgccccg
     3301 cgaaagccga gcatcgggtt ctcctcgtcg ggctcgtagc gctcgccgcc gatgagcttc
     3361 ttgtactcgt tcgacttgaa gtccgacagc cgcacgatca ccggcttcgg ccagaaagcc
     3421 gcggcgatgg tggcgacgcc ctcgaccatt ttctcgcgga agaaagtccg cgggtcggcg
     3481 tagccacggc tctttgcagc cagcttgctt ttcaattcat caggcagtgt ttcgtattcg
     3541 aggcaggcac gcggatgtgc cccgatctcg tggttgatga tgaactcgag ccgcgccagg
     3601 cccacccctg cgttgggcag ctgcgcgaac tcgaaggcga gctgcgggtt gccgacgttc
     3661 atcgcgatct tgaccggaat acgcggcatc tcgccgcgct cgcgcgctga cacctcgaaa
     3721 ggcagcaggc cctcgaacac gttgccggtg tcgccgtcgg cgcaggacac ggtgaccgcc
     3781 tggccgtccc tgaggcgcga ggtggcgtcg ccgcagccca cgacggcggg gatgccgagc
     3841 tcgcgcgcga tgatcgcggc gtggcaggtg cgcccgcccc ggttggtgac aatcgccgcg
     3901 gcgagcttca tcaccggctc ccagttcgga tcggtcatgt ccgtgaccag cacgtcgccc
     3961 tgcttcaccc gcgccatctg cgacgcatcg ggcacgaagc gcaccgtgcc gctgccgatc
     4021 ttgtggccga tggcgcgtcc gctcgcgagc acgcgcgagc tgcccttgag cgcgaagcgc
     4081 tgctcgactt cgcgctttcg cgacctgact gtctcgggcc gcgcctgcag cacgtagatg
     4141 cggccatcgg cgccgtcttt gccccattcg atgtccatcg gccgcccata gtggcgctcg
     4201 atgaccgccg cgtagcgtgc caattccaga acttcatcat ctttcaagga aaaggaattc
     4261 cgctccgcct cggcgacctc ggtcacgcgc gtcgaccgcc cggcggcggc ggactgcgaa
     4321 aacaccattt tctgcagctt ggagcctagc ctgcggcgca cgatcgccgg cttgccgcgc
     4381 tcgagcatcg gcttgtggac gtagaactcg tcgggattca ccgcgccctg gaccagcatc
     4441 tcgcccagcc cgtaggacgc tgtgatgaag accacgtcgc ggaagcccga ttcggtatcg
     4501 agggtgaaac tgacgccgct cgcgccgaga tcgctgcgca ccattcgctg gaccgcggcg
     4561 gagagcgcca caccctgctc gaagccctgg tgggcgcggt gggaaatcgc tcggtcgttg
     4621 tagagcgagg cgaagacatg ccggattgcc tccagcacgt tatcgacacc gcggatgttg
     4681 aggaaggtct cctgctggcc ggcgaacgag gcgtcaggca ggtcttcggc cgttgcggag
     4741 gaccgcacag catacgaggt atcacctgag gtctgcttct caagcgtctg aaaatatgac
     4801 cttatctctt gttcaaaggc ggcaggaaaa ggcgccttta aaatccatga gcggatttcc
     4861 gccccgcccg ctgcgagcgc ggtgacgtcc ttggggtcga gctttcgcag gcattgcgca
     4921 attggcgcat caagcccgct cgccgagagg aattcgcgga acgcgtcggc cgtggtggca
     4981 aagccgcccg gaacgcgaat gccggccgcg gtcaggctgc cgatcatctc gccaagggaa
     5041 gcgttcttgc cgccgacccg cggtaaatcc g
//
LOCUS       DASPXW010000066         2460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_24624, whole genome shotgun sequence.
ACCESSION   DASPXW010000066 DASPXW010000000
VERSION     DASPXW010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24624"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VEL04_01565"
     CDS             complement(<1..389)
                     /locus_tag="VEL04_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003806951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HYT47084.1"
                     /translation="MDFIRIRGARTHNLKNLSLELPRNRLVVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLQLMEKPDVDLIEGLSPAIAIEQKAASHNPRSTVGTVT
                     EIHDYLRLLYARVGTPYCPEHALALQA"
     gene            490..1686
                     /locus_tag="VEL04_01570"
     CDS             490..1686
                     /locus_tag="VEL04_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYT47085.1"
                     /translation="MSRAELRAGVSLAGVFGLRMLGLFLILPVFAVHAPQLAGGDNLL
                     LVGIALGAYGLSQAILHIPFGMASDRWGRKPVIYAGLVIFAAGSFLAAGADDIWTVIA
                     GRTLQGAGAISSAVVALAADLTREQHRTKTMAMIGASIGSVFALSLVAAPALYRWIGM
                     GGLFSLTGLLSLCAIWTVKRMVPDAAPSVHAAPSGGAAMLREALVQPDLLRLNLGIFV
                     LHLVQMAMFVVVPRMLVDAGLALDDHWMLYLPVVLLSFALMLPPVLYADRRNRAKPVL
                     LGAVALMLAVQVALAAAGGAPAALAALLVAFFAAFNVLEALLPALVSRLAPARSRGTA
                     IGVYNTTQTLGLFCGGLLGGAVAKNFGSGALFLGCAVLCGLWLAAAAGMRAPLLPGQR
                     TFESHV"
     gene            1717..2166
                     /gene="ssb"
                     /locus_tag="VEL04_01575"
     CDS             1717..2166
                     /gene="ssb"
                     /locus_tag="VEL04_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012354545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HYT47086.1"
                     /translation="MASVNKVIIVGNLGADPETRFLPSGEAVANIRVATTDRWKDKAS
                     GEMKEATEWHRISFFGRLAEIAGEYLKKGSQVYVEGSLRTRKWQDKDGQDRYSTDIRG
                     EVMQMLGKREGSGSVETREPAAPRAEAKPAAKKPAGKFDDMEDDIPF"
     regulatory      2187..2248
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
BASE COUNT          366 a          793 c          866 g          435 t
ORIGIN      
        1 tgcgcctgca gagccagagc gtgctcggga cagtacggcg tgcccacgcg cgcgtagagc
       61 agccgcaggt aatcgtggat ctccgtcacc gtgccgactg tggagcgcgg gttatggctc
      121 gccgccttct gctcgatggc aatcgccggc gacaggccct cgatcaggtc gacgtcgggc
      181 ttttccatca gctgcaggaa ctggcgcgcg tacgccgaga ggctctcgac gtagcggcgc
      241 tgcccttcgg cgtacagcgt atcgaaggcg agcgagctct ttcccgagcc cgacaggccg
      301 gtgatcacca ccaggcgatt gcgcggcagc tcgaggctga ggttcttcag gttgtgcgtg
      361 cgtgcgccgc ggatgcggat gaaatccatc agggcaggat gcggtccatt gacacgccga
      421 tgcaaaagcg aaacttgcta ctatagcgcg cttcgcattt ccgcctccag attttccatg
      481 aaagaaacga tgagccgcgc cgagctgcgc gccggcgtaa gcctcgcagg cgtgttcggg
      541 ctgcgcatgc tcgggctgtt cctcatcctg ccggtattcg ccgtgcatgc gccgcagctc
      601 gcgggcggtg acaacctgct gctcgtcggc attgcgctcg gcgcctacgg cctgtcgcag
      661 gcgatcctgc acattccctt cggcatggcg tcggaccgct gggggcgcaa gcccgtcatc
      721 tacgccggct tggtgatctt cgccgcgggc agctttctcg ccgccggggc ggacgacatc
      781 tggaccgtga tcgccggccg cacgctgcag ggcgcgggcg cgatctcatc ggcggtggtg
      841 gccctcgccg cggatctcac gcgggagcag caccgcacca agaccatggc gatgatcggc
      901 gcctcgatcg gcagcgtatt cgcgctctcg ctggtcgcgg cgccggcgct gtaccgctgg
      961 atcggcatgg ggggactgtt ctcgctcacc ggcctgctct cgctttgcgc gatctggacg
     1021 gtcaagcgca tggtgccgga cgcggcgccg agcgtgcacg cagcgccctc gggcggcgcg
     1081 gcgatgctgc gcgaggccct ggtgcagccg gatcttctgc ggctcaacct gggcatcttc
     1141 gtgcttcacc tggtgcagat ggcgatgttc gtcgtcgtgc cgcgcatgct ggtagacgcc
     1201 gggctcgcgc ttgacgatca ctggatgctg tacctgccgg tggtgctgct gtcgttcgcg
     1261 ctgatgctgc cgccggtttt gtacgccgat cggcgcaacc gggcgaagcc ggtgctcctc
     1321 ggcgcggtcg cgctgatgct cgcggtgcag gtcgcgctcg ccgcggcggg cggcgcgccc
     1381 gcggcgctgg ccgccctgct ggtggcgttt ttcgccgcct tcaacgtgct cgaggcgctg
     1441 ctgcctgcgc tggtctcgcg cctcgcgccg gcgcgctcgc gcggcaccgc catcggcgtg
     1501 tacaacacga cgcagacgct ggggcttttc tgcggcgggc tgctgggcgg cgcggtggcg
     1561 aaaaatttcg gcagcggcgc actgttcctc ggttgcgccg tactatgcgg tctgtggctc
     1621 gcggccgcgg ccgggatgcg tgcaccgctg ctccccggtc aacggacttt cgagtctcac
     1681 gtttaacact gcaggtggtg acgggggaga tttgatatgg catcggtcaa caaggtgatc
     1741 atcgtcggca acctgggcgc cgacccggaa acgcgctttc tgcccagcgg cgaagcggtg
     1801 gccaacatcc gcgtggcgac caccgaccgc tggaaggaca aggcgagcgg cgagatgaag
     1861 gaggcgaccg agtggcaccg catctcgttt ttcggccgcc tggcggagat cgccggggag
     1921 tacctgaaga aaggctcgca ggtctacgtc gagggcagcc tgcgcacgcg caagtggcag
     1981 gacaaggacg gccaggatcg ctatagcact gacatccgcg gcgaggtgat gcagatgctc
     2041 gggaaaaggg aaggcagcgg cagcgtcgag acgcgcgagc ccgcggcgcc gcgcgccgag
     2101 gcgaagcccg cggccaagaa acccgccggc aagttcgacg acatggagga cgacatcccg
     2161 ttctgatgct tcgggtatca ttccaaccga agcagggaga tggcatacct cccctaaccg
     2221 ccgcaaggct gatgatgcct acgcgagacg cctcggaagg gcggcagcgt aggcattttt
     2281 gtatccctcc gatgcgtcgc acgcggtacg acgaccgtcg gaggtattgc aatgactgaa
     2341 acctgggtgc tcgcggcgct gtggatcggc ctcgggctgt tcgccgcgct cgcttcgata
     2401 tggctgcgcg tcgccaccgc gctcaccgag atcgtagtcg gggcgctcgc gcagggcgtg
//
LOCUS       DASPXW010000067         3398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_24668, whole genome shotgun sequence.
ACCESSION   DASPXW010000067 DASPXW010000000
VERSION     DASPXW010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24668"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VEL04_01580"
     CDS             complement(<1..277)
                     /locus_tag="VEL04_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HYT47087.1"
                     /translation="MLVAFAAFLGLGFAAFTTLNIEAYPDPAPPIVEIIAQRPGQSPE
                     EMERYVTIPIEIAVASTPGLRYIRSNTVYALSFIRLQFEYGRDYHFVR"
     gene            575..1063
                     /locus_tag="VEL04_01585"
     CDS             575..1063
                     /locus_tag="VEL04_01585"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HYT47088.1"
                     /translation="MAVQVDMNIELKLKVEDLNARYAQAIDDDKLEAWPNFFTEQGRY
                     RVTTAENFERGLPLGMIYATSRAMLRDRVRSLREANVYEAQRYRHVIGVPLIAPAEGS
                     AVRAQTSFIVARIMHSGETTLFATGRYHDHIVLDDGEARFAEKIVILDSRLIDTLLAI
                     PF"
     gene            1074..2309
                     /locus_tag="VEL04_01590"
     CDS             1074..2309
                     /locus_tag="VEL04_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYT47089.1"
                     /translation="MPEPAAALLTRHRSFVLFWCARTFTNGAYMMQGVAVGWQIYELT
                     NDPFDLGLVGLVQFVPLVALSVVIGQVADRFDRRIVVATCQVGKALAALALALGTARG
                     SLTRDAMLAILFVSGTARAFEMPSIHALLPGLVPVTLLPRAIAASVSAQQTAVICGPS
                     LGGLLYMLSPTGVYATCTAVFIAASVLISLVHVTRRSDERKPINLETLFAGFRYIRSR
                     PILLGAISLDLFAVLLGGVTALLPIYARDILQAGPWALGLLRSAPAVGALATSVVLAR
                     RAIGGSAGRIMFAAVGTFGLASLAFGLSTSVALSFLALVIYGASDAISVVIRQSLVQL
                     RTPYDMLGRVLAVNSMFTGSSGSLGEFRAGAVAAALGAVPSALIGGLGAIVVMLIWIW
                     AFPQLRRVDKLTPEAGAAL"
     gene            2663..>3398
                     /locus_tag="VEL04_01595"
     CDS             2663..>3398
                     /locus_tag="VEL04_01595"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HYT47090.1"
                     /translation="MAEPSRPARLWYQSFVHPIEQAPYIERLQALLDAAASPGIRFEV
                     HGLDPPDHLFHPLTEFRCAAQTIRNALDAERAGYDAFVIGHFQEPGLLEIRGAVDIPV
                     IGLGEATLLAALSMGRQLGLVTIDPVFIDWHERQVRAHGLEQRVTGVRAIHADLAGFM
                     RAFTDDASYAQVRADFVEQVRPLVAAGAEVIIPAGGLPMLLFARERPFIIDGALVLNG
                     IAVVAKAAEMALALRRLTGSVVSRRGT"
BASE COUNT          516 a         1124 c         1121 g          637 t
ORIGIN      
        1 ggcgtacgaa gtggtagtcg cggccgtatt cgaactgcag ccgaatgaaa ctgagcgcgt
       61 agacggtgtt ggagcggatg tatctgagcc caggcgtgct ggcgaccgcg atctcgatcg
      121 ggatcgtgac gtaacgttcc atctcctcgg gcgactgccc tggccgctgc gcgatgatct
      181 cgactatcgg gggcgcagga tcgggatagg cctcgatgtt gagtgtggtg aaggccgcaa
      241 agcctagccc gagaaatgcc gcgaacgcga ccagcacgag cggccgtcgc aacaggcaaa
      301 aggcaatcag gccacgcagc atagtgcttc gatcggctca gggattgact ggccgcagtt
      361 ggccagtgcc accgcgcaga acgcaatcag accgttcgtc gcagtttgcc cccgatcgcg
      421 gactttcagc aagatcaggc tgcgcgcctt ttgtgagtcg acgctctagt attcggaatc
      481 acaatgcgct gagatgttca gccgcaagcg taaccgtcgt tagagcccga attcggtgcg
      541 cgagagggcc cgagcggcgc cgtcggcgtc cgtggtggcg gtccaggttg acatgaacat
      601 cgagctcaag ctcaaggtcg aagacctcaa cgcccgctat gcccaggcga tcgacgacga
      661 caagctcgaa gcatggccga atttcttcac cgagcagggc cgctaccgcg tcaccacggc
      721 ggagaatttc gagcgcggcc tgccgctcgg catgatctat gcgacctcgc gcgccatgct
      781 gcgcgaccgc gtgcgctcgc tgcgcgaggc caacgtctac gaggcccagc gctatcgcca
      841 cgtgatcggc gtgccgctga tcgctcccgc cgagggcagc gccgtgcggg cgcagacgag
      901 cttcatcgtt gcccggatca tgcatagcgg cgaaaccacg ctgttcgcca ctggccgcta
      961 tcacgaccat atcgtgctcg acgacggcga ggctcgtttt gccgagaaga tcgtgatcct
     1021 cgacagccgg ctgatcgaca cattgctcgc cattccattc tagcccgcac cccatgcccg
     1081 agcccgccgc cgccttgttg acgcgccacc gttcgttcgt gctgttctgg tgcgcgcgca
     1141 ccttcaccaa cggcgcctac atgatgcagg gggtagcggt aggctggcag atctacgagc
     1201 tcaccaacga tccgttcgac ctcggcctgg tcgggctggt gcaattcgtg ccgctggtgg
     1261 cattgtcggt tgtgatcggg caggtcgccg accgcttcga tcggcgcatc gtcgtcgcta
     1321 cctgtcaggt gggcaaagcg ctggcggcgc tcgcgcttgc gctcggcacc gcgcgcggct
     1381 cgctcacccg cgacgccatg ctggccatcc tgttcgtgtc gggcaccgcg cgcgcgttcg
     1441 aaatgccgag tatccacgcg ctgctgcccg gcctcgtgcc ggtgacgctg ttgccgcgcg
     1501 cgatcgccgc ctcggtgagc gcgcagcaga ccgccgtcat ctgcgggccc tcgctcggcg
     1561 ggctgttgta tatgctcagc cccaccgggg tctacgcgac ctgcaccgcg gtcttcatcg
     1621 cggcgagcgt gctgatcagc ctcgtccacg tgacgcggcg cagcgacgag cgcaaaccga
     1681 tcaacctgga gacgctgttc gccggctttc gctatatccg cagccgtccc atcctgttgg
     1741 gcgcgatctc gctcgacctg ttcgccgttc tgctcggcgg ggtgaccgcg ctgttgccga
     1801 tctacgcccg cgacatcctg caggccgggc cgtgggcgct cggcctgttg cgctcggcgc
     1861 cggcggtcgg ggcgctcgcc acctcggtcg tgctggcgcg ccgcgccatc ggcggcagcg
     1921 ccggacgcat catgtttgcc gcggtcggaa cgttcgggct agcgagcctc gctttcggac
     1981 tgtcgacctc ggtcgcgctg tcgttcctcg cgctcgtgat ctacggcgcc tccgacgcga
     2041 tcagcgtcgt catccgccag tcgctggtgc agttgcgcac cccctatgac atgctgggcc
     2101 gggtgctggc ggtcaactcg atgttcacgg gttcctcggg ctcgctcggc gaatttcgcg
     2161 ccggcgccgt cgcggcggcg ctgggcgcgg tgccgtccgc gctgatcggc ggcttgggcg
     2221 cgatcgtggt gatgctgatc tggatatggg cgttcccgca acttcgccgc gtcgacaaat
     2281 tgacgccgga ggcgggagcc gcgctgtgac cgcgacgggc gcgagcgcag ccctcgagtg
     2341 tccgccaagc gggcatcgcg gaagagatgg actcgggctg agttcttcgg agcatctcac
     2401 ccgctcattc ccgcgcatag cgcgtcgaag acgcgcgtga acgcgcttat gctgggaatc
     2461 caggcgaggg ctgggtcccg gctttcgcga ggacgagcgg agagaaaatt cgtcttggtt
     2521 tgagccgctg cgactttcgc tgttcgcgcc gccgatgatg ctcttttccc aatttgaacg
     2581 attgcctaat cttccgcagc gtgtgtgggc gatgggacaa agcgcgtcgc ccaggcgcag
     2641 tgttgatgct ggaggatagc gtatggccga accttcccgt cccgcccggc tgtggtatca
     2701 gagcttcgtc catccgatcg agcaggcgcc ctacatcgag cggctgcagg cgctgctcga
     2761 cgcagccgcc agtcccggca tccggttcga ggtccatggc ctcgatcctc ccgaccacct
     2821 cttccatccg ctgacggaat tccgctgcgc ggcgcagacc atccgcaatg ccctggacgc
     2881 ggagcgcgca ggatacgacg ccttcgtcat cggtcacttc caggaacccg gcctgttgga
     2941 aatccgcggt gcggtcgaca tccccgtgat tggtctcggc gaagcgacac tgcttgcggc
     3001 attgagcatg gggcgccaac ttggtctcgt gaccatcgat ccggtcttca tcgactggca
     3061 tgagcggcag gtgcgagcac acggcctcga gcagcgcgtc acgggcgtgc gggcgattca
     3121 cgcggacctc gctggtttca tgcgcgcctt caccgacgat gcaagctacg cccaggtccg
     3181 cgccgatttc gtggaacagg ttcgtccgct ggtcgcggcg ggggcggagg tcatcatccc
     3241 ggcaggcggc ctgccgatgc tgctgtttgc gcgcgagcgc ccattcatta tcgacggcgc
     3301 gctggtgctc aacggcatcg cggtagtggc aaaggccgcg gagatggcgc ttgcgctacg
     3361 ccgcctcacc ggctcggtgg tcagccgccg cggtactt
//
LOCUS       DASPXW010000068         2934 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_779710, whole genome shotgun sequence.
ACCESSION   DASPXW010000068 DASPXW010000000
VERSION     DASPXW010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779710"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VEL04_01600"
     CDS             <1..404
                     /locus_tag="VEL04_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014616234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HYT47091.1"
                     /translation="KAQQRGLKPIPALYQAMDAVLDIQTDKLAIPRRFTAVMKEIWAL
                     QPRFEQRSGRRPFALMTHERFRAGYDFLVLRAASGEAPAELAQWWEKFQHAREDERNA
                     MLLAPQPDEHRRRRRRRRRKAAPLPPDAAPA"
     gene            406..894
                     /gene="folK"
                     /locus_tag="VEL04_01605"
     CDS             406..894
                     /gene="folK"
                     /locus_tag="VEL04_01605"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HYT47092.1"
                     /translation="MPSVKAYVGLGANLGEPEAQVRSALTALEAIPGTRLLAASSLYR
                     SAPVGVGEQPDFINAVAAIDTRLSARALLDELLGIEARFGRRRESPGAPRTLDLDLLL
                     YGDQLIDEPGLVVPHPRMHGRAFVLAPLAEIAPDTVVPGKGPVATLLAACKDQNVERI
                     RG"
     gene            1018..>2934
                     /locus_tag="VEL04_01610"
     CDS             1018..>2934
                     /locus_tag="VEL04_01610"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYT47093.1"
                     /translation="MSAETLLETLRQLAAELHPARQAPPASLDARLEHDYGFDSLGRV
                     ELFLRIERRFGVSLPESVMGSAETPRDLLRAALAAGAAHPSHAAAVGRAAALAAESAA
                     PDEARTLAEVLEWHLARHSDRLHLVLQDDQGREQSVTYAQLHGEARAIAAGLHERGLE
                     PGRAVAIMLPTSAEYFYSFLGVLLAGGIPVPIYPPARASQIEEHVRRHVGILSNALTE
                     FLITVPQAKPVVLLLKPRVATLKSVVTPQELARPGASGTPYKPKPSDIAMLQYTSGST
                     GNPKGVILTHQNLLTNIRAMGHALRVTPNDVFVSWLPLYHDMGLIGVWMGSLIYGFKC
                     PVMSPLSFLARPDRWLWSIHHHAGTLSAGPNFAYELCLRKVQDSDIQGLDLSSWRFAF
                     NGAEPVSPETIDAFIKRFARYGFKPGAMAPVYGLAEATLGLAFPPPGRGPKVDRVERD
                     SFTRGARAVPASESASNALSFVACGLPLPGHQIRIVDASDRELGEREEGHIQFSGGSA
                     TSGYFRNPDETRRLLHGEWVDTGDYGYIAEGEIYITGRVKDVIIHAGRNIYPYELEEA
                     VSELEGIRKGCVAVFGAKDARFGTERIVVLAETRERDPARREQLQARINELAVSLIGM
                     PVDDIVLASPHTVLK"
BASE COUNT          474 a         1075 c          955 g          430 t
ORIGIN      
        1 aaaaagccca gcagcgggga ctgaagccga tcccggcgct ctaccaggcg atggacgcgg
       61 tgctcgacat ccagaccgac aagctcgcca tcccgcgccg cttcaccgcc gtgatgaagg
      121 agatctgggc gctgcagccg cgcttcgagc aaaggagcgg ccgccgcccc ttcgccctca
      181 tgacgcacga aagattccgc gccggatacg atttcctcgt cttgagggcc gcaagcggcg
      241 aggcaccggc agagctcgca caatggtggg agaaattcca gcacgcgcgc gaagacgagc
      301 gcaacgccat gctgcttgcg ccgcagccgg acgagcaccg gcgccgccgg cgcaggcggc
      361 gcaggaaggc ggcgcctctg ccgcccgacg ccgcaccggc gtgaaatgcc gtcggtcaag
      421 gcctacgtcg ggctcggggc gaacctcggc gagcccgaag cccaggtgcg cagcgccctc
      481 accgcgctcg aagccattcc agggactcgc cttctcgccg cctcctcgct ctaccgcagc
      541 gcgccggtcg gcgtcggcga gcagcccgac ttcatcaacg cggtcgccgc aatcgatacg
      601 cgcttgagcg cccgggcgct gctcgatgag ctgctcggaa tcgaggcgcg cttcggccgc
      661 aggcgcgagt cccccggggc cccgcgcacg ctcgacctcg accttctcct ctacggcgat
      721 cagcttatcg acgagcccgg gctggtcgtt ccccacccgc gcatgcacgg gcgcgcattc
      781 gtgctcgcgc cgctcgccga aatcgcacct gacaccgtcg ttccgggcaa agggccggtc
      841 gcgacgcttc tcgccgcctg caaggatcaa aacgtggaga gaatccgggg ctgacgggac
      901 cgccccgcct caagcccgcg ccgcacgggc tgtgctaaca tcgcccgctc ccttcgatcg
      961 ccgggcccgt gcgtggaaac ttccgtcgct aggcaagaca ggggcggcgg cgctccggtg
     1021 agcgccgaaa cgctgctcga gaccctgcgg cagctcgccg ccgagctgca tcccgcgcgt
     1081 caggctccac cggcgtcgct cgacgctcgc ctcgagcacg attacggatt cgacagcctc
     1141 ggacgcgtgg agctctttct gcgcatcgaa cggcgcttcg gcgtgagtct tccggaaagc
     1201 gtcatgggaa gcgccgagac cccgcgcgac ctgttgcgcg ccgcgctcgc cgcgggagcc
     1261 gcgcacccct cgcacgccgc ggcagtcggg cgcgctgcgg cgctcgctgc ggaatccgct
     1321 gcgcccgacg aggcgcgcac gctcgccgaa gtgctcgagt ggcacctcgc ccggcactcc
     1381 gaccgcctgc atctcgttct gcaggacgac cagggacgcg agcagtccgt tacttacgcg
     1441 cagctccacg gcgaggcccg cgccatcgcc gcagggctcc acgagcgcgg cctggagcct
     1501 ggccgcgccg tagccatcat gctgccgacc agcgccgaat atttctacag cttcctcggc
     1561 gtcctgctcg cgggaggcat acccgtgccg atctatccgc cggcgcgcgc ttcacagatc
     1621 gaagagcatg tgcgccggca cgtcggcatt ctctccaacg cgctcaccga attcctcatc
     1681 acggtgccgc aggcgaagcc cgtggtgctg ctgctcaagc cgcgagtcgc gactctcaag
     1741 agcgtcgtga cgccgcagga gctcgcgcgg ccgggagcgt cgggcacgcc ctacaagccc
     1801 aagcccagcg acatcgcgat gctccagtac acctccggga gcacgggcaa tcccaagggt
     1861 gtcatcctca cccaccagaa cctcctcacc aatatccggg cgatgggcca cgcgctgcgc
     1921 gtgacgccca acgacgtgtt cgtgagctgg ctgccgctct accacgacat ggggttgatc
     1981 ggcgtgtgga tgggcagcct catatacggc ttcaagtgcc cggtgatgtc gccgctcagc
     2041 ttccttgccc ggccggaccg ctggctgtgg tccatccacc accacgcagg cacgctctcg
     2101 gccgggccga atttcgccta cgagctgtgc ctgcgcaagg tgcaggactc ggacatccag
     2161 ggcctggatt tgagcagctg gcgttttgcg ttcaacggcg ccgagcccgt gtcgcccgag
     2221 accatcgacg cgttcatcaa gcgcttcgcc cgctacggct tcaagcccgg cgcgatggcg
     2281 cccgtatatg gactcgccga ggccacgctg gggctcgcgt ttccgccgcc ggggcgcggc
     2341 ccgaaagtcg atcgcgtcga gcgcgacagc ttcacccggg gcgctcgggc ggttccggcc
     2401 tccgaatccg cgtccaacgc gctgtccttc gtcgcgtgcg gactgccgct gcccggccat
     2461 cagatccgga tcgtcgatgc gtccgaccgc gagctcggcg agcgcgagga agggcacatc
     2521 cagttcagcg gaggctccgc gacgagcggc tatttccgca atccggacga aacgcgccgc
     2581 ctgctgcacg gggaatgggt ggacaccggc gactacggct acatcgccga gggcgagatc
     2641 tacatcaccg gccgcgtcaa ggacgtcatc atccacgcag gccgcaacat ctatccctac
     2701 gagctggagg aagcggtgag cgagctggaa ggcatccgca aagggtgcgt ggcggtgttc
     2761 ggcgcgaagg acgcgcgctt cgggaccgaa cgcatcgtgg tgctcgccga gacccgcgag
     2821 cgggaccccg cgcggcgcga gcagctgcag gcccgcatca acgagctcgc ggtttccctg
     2881 atcggcatgc cggtggacga catcgttctc gcgtcgccgc acaccgtgct caag
//
LOCUS       DASPXW010000069         5855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1286717, whole genome shotgun sequence.
ACCESSION   DASPXW010000069 DASPXW010000000
VERSION     DASPXW010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5855
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1286717"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..1575)
                     /gene="gshA"
                     /locus_tag="VEL04_01615"
     CDS             complement(64..1575)
                     /gene="gshA"
                     /locus_tag="VEL04_01615"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HYT47094.1"
                     /translation="MTIQERLRGLAPEALKGMRRGIEKESLRVRPDGSLSAAPHPQAL
                     GSALTHPHITTDFSESQLELITGVHEGVDACLEELTGIHQMVYRAIGDEALWCASMPC
                     NLPADDAIPIGRYGASNVGRAKTVYRTGLSHRYGRRMQTISGIHYNLSMPGVTSEQYF
                     ALIRNFRRRSWLLLYLFGASPAVCSTFVAGRQHELQALGPGTLYAPHGTSLRMGRLGY
                     QSAAQASLEVSYNSLKSYTASLYDALTKPYPPYEKIGILAGGEYRQLNTTLLQIENEF
                     YGTIRPKRRIRRGERPLHALRERGVEYVEVRLMDLDPFVAIGIGASTCRLLDVFLLHC
                     LLDDSPPDTPQEIAAIKTNQQRVAVRGREPGLKLERDSEEVSVSQWGTEVLDQCRPIA
                     EALDRLHGGAAFRDALLAAGAVVKDPGATPSARVLGAMARNHGDTYVRFVLSESLLHA
                     GHLRGLPLDPRVERRFAHLAGESLAEQARIEAGDKVDFETFRKQYLSPDLLKV"
     gene            1669..1878
                     /locus_tag="VEL04_01620"
     CDS             1669..1878
                     /locus_tag="VEL04_01620"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HYT47095.1"
                     /translation="MEVDVKYRIALNKSEEGYSVSVPGLPGCWSQGATEHEAVENIRD
                     AIREYLSVVNEQLRGADVREIEVVI"
     gene            1991..3952
                     /locus_tag="VEL04_01625"
     CDS             1991..3952
                     /locus_tag="VEL04_01625"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HYT47096.1"
                     /translation="MTSATAFERLNDKQREAARFGSGPLLILAGAGTGKTNTLAHRVA
                     HLVLEGAAPERILLLTFSRRAALEMTRRAQRIVGEVVKNVRLPWSGTFHSVANRLIRR
                     NASRVGLDASFSVLDRGDAADLMDVVRHDLGLSKTEKRFPRKDTCLAIYSHRVNTRDA
                     LAGTLGMFPWCAEWEGELNRLFRNYVERKLENQALDYDDLLLYWHAMMADERLAAEIG
                     GAFDHILVDEYQDTNLLQAEILRGLRPGGEGVTVVGDDAQAIYSFRAATVENIRNFSS
                     AYQASVVTLEDNYRSTQPILDAANALTGKGLVSSKGKGDKPRYVTVADDAAQAGYVVS
                     QVLQHREEGIALKRQAVLFRSSHHSDVLELELVRRNIPYVKYGGLKFLEAAHVKDLLA
                     VLRWADNPKNRLAAWRALQLLPGVGPATAGHSFARFELQGWKALHDLDAALEALMHSL
                     AAPETPWAGQVGRVREWFEPHLERIYEEAQVRAGDLLQLERISINFSTREEFLSELAL
                     DPPAATGDFSGAPLLDEDYLVLSTVHSAKGQEWDAVYVLNVADGSFPSEFATGRAELI
                     EEERRLLYVAMTRAKSSLDLMAPVKYYVTQQSRMGDRHVYGARSRFITGAVMACLEQI
                     GWSDASTAGERAGRGTHSVDVAAKLRGMW"
     gene            3966..4832
                     /locus_tag="VEL04_01630"
     CDS             3966..4832
                     /locus_tag="VEL04_01630"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HYT47097.1"
                     /translation="MGALALLLGLMAVGFVAAGDGRSYHGPKLIEEQVRIPAAGGRYE
                     IAATILRPEGAGPYGAMVLNHGVSASARERAEESSELLIGAASVFARRGYVVVMPLRR
                     GFGRTGGEMAEDPGSCADPDFRKAEQAASDDVMAAYDYARALPYVDASRMILAGQSAG
                     GMASLYTAGTRSPQGLIAVLSFAGGRGGDPDSGAGVPCAVEPVAKLLDALGKTTRVPV
                     LFHYSENDLFFSPKTSRLWFERYAAGGADAEYALQPAFGKNGHYLFSDVAGVRSWLPT
                     VEQFFARHGLPF"
     gene            complement(4878..5033)
                     /locus_tag="VEL04_01635"
     CDS             complement(4878..5033)
                     /locus_tag="VEL04_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47098.1"
                     /translation="MCYEFEYWTQRQRAEEARKELLKKAEERAKQKPVEPALPETGVK
                     QPDPVPV"
     gene            5086..>5855
                     /locus_tag="VEL04_01640"
     CDS             5086..>5855
                     /locus_tag="VEL04_01640"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HYT47099.1"
                     /translation="MEAVRDDSPEAKEEALRDDRRLLGRLLGEVIREQVGAGMLERIE
                     RIRQAAVGFRRAASEKSELDALLNALDTEQTLHVVRAFSFFSHLLNIAEDEQQHRRRR
                     AHAAAGSPRRPGSLSFALDQVRSLGFDPLMAWFGRAHVSPVLTAHPTEVQRQSILDCE
                     REIARLLSRSQNSERDEALHAEILRLWLTAMLRRARLAVADEIANGIAYFRLTFLNEL
                     PRLYAEFEHALKERYGLERPPRLAPFLTVSSWIGGDRDG"
BASE COUNT          948 a         1915 c         2038 g          954 t
ORIGIN      
        1 ggagtggacg gtcatgggca atgtcataag aaaaatcagc atgaagcgag caatcattgc
       61 ccatcaaacc tttagcaggt cgggtgagag gtactgcttg cggaaggtct cgaagtcgac
      121 cttgtcgccg gcctcgatgc gcgcctgctc ggcaagcgac tccccggcga ggtgggcgaa
      181 gcgccgctcg accctgggat ccagcggcag gccgcgcagg tggccggcgt gcagcagcga
      241 ctccgagagc acgaaacgca cgtaggtgtc gccgtggttg cgcgccatgg cgccgagcac
      301 gcgcgccgag ggcgtggcgc caggatcctt caccactgcg ccggcggcga ggagcgcgtc
      361 gcggaaggcg gctccgccgt gcaggcggtc tagcgcctcg gcgatgggcc ggcactggtc
      421 cagcacttcg gttccccact gggaaacgga cacttcttcg gagtccctct ccagcttcag
      481 gccgggctcg cggccgcgca cggcgacgcg ctgctggttc gtcttgatcg ccgcgatctc
      541 ctgcggcgta tcgggcgggc tgtcgtcgag caggcagtgc agcaggaaga cgtcgagcag
      601 gcggcaggtg gacgcgccga tgccgatggc gacgaagggg tcgaggtcca tcaggcgcac
      661 ttcgacgtac tcgacgccgc gctcgcgcag ggcgtgcagc ggccgctcgc cgcgcctgat
      721 gcggcgcttg gggcggatgg tgccgtagaa ctcgttctcg atctgcagca gcgtggtgtt
      781 gagctggcgg tactcgccgc cggccaggat gccgatcttc tcgtagggcg gatacggctt
      841 ggtcagcgca tcgtagagcg aggcggtgta gctcttcagg ctgttgtagc tcacttcaag
      901 cgaggcctgg gcggcgctct ggtagcccag gcgccccatg cgcagcgagg tgccgtgcgg
      961 cgcgtacagg gtcccgggcc cgagcgcctg cagctcgtgc tggcggccgg cgacaaaagt
     1021 ggagcatacc gcgggcgagg cgccgaagag atagagcagc agccacgagc ggcgccggaa
     1081 gttgcggatc agcgcgaagt actgctcgct cgtcacgccg ggcatcgaga ggttgtagtg
     1141 gatgcccgag atggtctgca tgcgccggcc gtagcggtgc gagagcccgg tgcggtacac
     1201 ggtctttgcg cgcccgacgt tggaggctcc gtagcgcccg atcgggatgg cgtcgtcggc
     1261 gggcaggttg cacggcatcg acgcgcacca cagcgcctcg tcgccgatgg cgcggtacac
     1321 catctggtgg atcccggtca gctcctcgag gcaggcgtcg acgccttcgt gcacgccggt
     1381 gatgagctcg agctgcgact cgctgaagtc ggtggtgatg tgcgggtgcg tgagcgccga
     1441 gccgagcgcc tgcggatgcg gcgcggcgga gagcgagccg tcgggccgca cccgcaggct
     1501 ctccttctcg atgccgcgcc gcatgccctt cagcgcctcg ggcgcgagcc cgcgcagccg
     1561 ttcctggatg gtcatggaga gctagaaggt aacttgaaaa cagggacaga ccacgttttc
     1621 tctcgaaacc ggggtcagag cccaattgac cgcgggctta taatcgcggt ggaggtcgac
     1681 gtgaaatatc ggatcgcact aaacaagtcg gaggaagggt acagcgtttc cgtcccgggg
     1741 cttccgggct gctggtccca aggcgcaacc gagcatgaag cagtggaaaa catccgcgac
     1801 gccatccgcg aatatctttc cgtcgtaaat gagcagcttc gaggtgccga cgtccgagaa
     1861 attgaagtag tcatctagtc ttgcccaaga tccccggcgt caatcacctt gacgccgtgc
     1921 gcgcattgca aaaggccgga ttccgcgtgg ggctggacga atatacagta ttcgcatatt
     1981 gtgtcgcccc gtgaccagcg ccaccgcctt cgagcggctc aacgacaagc agcgcgaagc
     2041 ggcgcgcttc ggcagcgggc cgctgctgat cctcgccggc gccggcaccg gcaagaccaa
     2101 cacgctcgcg caccgcgtcg cgcacctcgt gctcgagggc gcggcgcccg agcgcatcct
     2161 gctgctcact ttctcgcggc gcgcggcgct cgagatgacg cgccgggcgc agcgcatcgt
     2221 cggcgaggtg gtgaagaacg tgcggctgcc ctggtcgggc accttccact cggtcgcgaa
     2281 ccgcctcatc cgcaggaacg cctcgcgcgt cgggctcgac gcgagcttca gcgtgctcga
     2341 ccgcggcgac gcggcggacc tgatggacgt ggtgcgccac gacctcggtc tctccaagac
     2401 cgagaagcgc ttcccgcgca aggacacctg cctcgcgatc tactcgcacc gcgtgaatac
     2461 tcgggacgcg ctcgccggca cgctcggcat gttcccgtgg tgcgcggaat gggagggaga
     2521 gctgaaccgg ttgttcagga attacgtcga aagaaagctc gagaaccagg cgctcgacta
     2581 cgacgatctc ctgctctact ggcacgcgat gatggccgac gagcgcctcg cggccgagat
     2641 cggcggcgcc ttcgaccaca tcctggtcga cgaataccag gacacgaacc tgctgcaggc
     2701 ggagatcctc aggggcctgc gcccgggcgg cgagggcgtg acggtggtgg gcgacgacgc
     2761 gcaggcgatt tattcgtttc gcgccgcgac ggtcgagaac atcaggaatt tttcgtccgc
     2821 gtaccaggca tcggtggtaa ccctggaaga caattaccgc tcgacgcagc ccatcctcga
     2881 cgccgccaat gcgctgacag gaaaaggcct ggtctcgagc aaaggcaaag gcgacaagcc
     2941 gcgctacgtc accgtggcgg acgacgcggc ccaggcgggt tacgtggttt cgcaggtcct
     3001 gcagcacagg gaagaaggca tcgcgttgaa gcgccaggcg gtgctgttcc gcagctcgca
     3061 ccactccgat gtcctcgagc tggagctggt gcgccggaac atcccgtacg tgaagtacgg
     3121 cggcctcaag ttcctcgagg cggcgcacgt caaggacctg ctggcggtgc tgcgctgggc
     3181 ggacaacccg aagaaccgcc tcgctgcctg gcgcgcgcta cagctcctgc cgggcgtcgg
     3241 cccggcgacg gcggggcata gcttcgctcg ctttgaattg caggggtgga aggctttgca
     3301 tgacctggac gccgcacttg aagcgctgat gcattcgctg gcggcgcctg aaaccccctg
     3361 ggcggggcag gtcgggcgcg tgcgcgagtg gttcgagccg cacctcgagc gcatctacga
     3421 agaggcccag gtgcgcgccg gggatcttct ccagctcgag agaatttcaa taaatttttc
     3481 caccagagag gaattcctga gcgagctcgc cctggacccg cccgccgcga ccggcgactt
     3541 ctcgggcgcg ccgctgctcg acgaggacta cctcgtgctt tcgaccgtgc actcggcgaa
     3601 aggccaggag tgggacgcgg tctacgtgct gaacgtcgcc gacggcagct tcccgtccga
     3661 attcgccacc ggccgcgccg agctgatcga ggaagagcga cggctgctct acgtcgcaat
     3721 gacgcgggcg aaatcgagcc tggacctgat ggcgccggtc aagtattacg tgacgcagca
     3781 gtcgaggatg ggcgatcgcc acgtctacgg cgcgcgcagc cgcttcatca cgggcgccgt
     3841 catggcttgc ctggagcaga tcggctggag cgacgcatcc acagcaggcg agcgcgccgg
     3901 ccgcggcacg catagcgtgg atgtcgcggc gaaattgaga ggcatgtggt aattttgtag
     3961 cctctatggg cgcgctggcg ctgctgcttg gcctcatggc cgtcggattc gtggcggccg
     4021 gcgacgggcg ttcgtaccat ggccccaagc tcatcgaaga gcaggtgcgc atcccggcgg
     4081 ccggcggccg ctacgagatc gccgccacca tcctgcgtcc cgaaggcgcc ggcccctacg
     4141 gcgcgatggt gctcaatcac ggcgtctcgg cgagcgcgcg cgagcgcgct gaggaatcct
     4201 cggagctcct gatcggcgcc gcctcggtgt tcgcgcgccg cggctacgtg gtggtgatgc
     4261 cgctgcggcg cggcttcggc cggaccggcg gcgagatggc ggaggacccg ggcagctgcg
     4321 ccgatcccga tttccgcaag gccgagcagg ccgcttccga cgacgtcatg gccgcctacg
     4381 actacgcgcg cgcgctaccg tacgtcgacg cctcgcgcat gatcctcgct ggtcaatcgg
     4441 cgggcggcat ggcgtcgctc tacaccgcgg gcacgcgctc gccgcagggc ctcatcgcag
     4501 tgctttcctt cgccggcggc cgcggcggcg atcccgactc gggcgcgggc gtgccctgcg
     4561 cggtcgagcc ggtcgcgaag ctgctcgacg cgctgggcaa gacgacgcgc gtgccggtgc
     4621 tcttccacta ctccgagaac gatctcttct tcagcccgaa gacttcgcgg ctctggttcg
     4681 agcgctatgc ggccggcggg gccgatgcgg agtacgcgct gcagcccgcc tttggcaaga
     4741 acgggcacta cctcttcagc gacgtcgcgg gcgtgcgctc ctggctgccc acggtagagc
     4801 agttcttcgc gcgccacggc ctgccgtttt agcggctgga ttccgccgtg gacgaccccc
     4861 ttgtcagggg gtcggggtca aacaggaaca ggatcgggct gcttgactcc ggtctcgggc
     4921 agcgccggct cgaccggctt ctgcttcgcg cgctcctcgg ccttcttcag tagctccttg
     4981 cgcgcctctt cggcgcgttg ccgctgggtc cagtactcga actcgtagca catgaggaat
     5041 ctcctgtcgg ttcgagctgc tgatcctacg ccttagaatc gtcgcatgga agcggtgcgg
     5101 gacgactcgc cggaagccaa ggaggaggcg ctgcgcgacg accggcggct gctcgggcgg
     5161 ctgctgggcg aggtgatccg cgagcaggtg ggcgccggga tgctcgagcg catcgagcgc
     5221 atccgccagg cggcggtcgg cttcaggcgc gcggcaagcg agaaatccga gctcgacgcg
     5281 ctgctgaacg cgctcgatac cgagcagacg ctgcacgtgg tgcgggcgtt cagcttcttc
     5341 tcccacctcc tcaacatcgc cgaggacgag cagcagcacc gccgccgccg cgcgcacgcc
     5401 gcggcgggct cgccgcgccg ccccggcagc ctgtcattcg cgctcgacca ggtgcgcagc
     5461 ctcggtttcg acccgctgat ggcatggttt ggccgggcgc acgtcagccc cgtgctcacc
     5521 gcgcatccga ccgaagtaca gagacagagc atcctggatt gcgaacggga gatcgcccgc
     5581 cttctttcca gaagtcagaa ttcagaacgc gacgaggcgt tgcatgcgga aattctgcgg
     5641 ctgtggctca ccgcgatgct gcgccgcgcg cggctcgcgg tcgccgacga gatcgccaac
     5701 ggcattgcct acttcaggct cactttcctg aacgagctgc cgcggcttta tgccgagttc
     5761 gagcacgcgc tgaaggagcg ctacggcctc gagcggccgc cgcggctcgc gccctttctc
     5821 accgtgtcga gctggatcgg cggcgaccgc gacgg
//
LOCUS       DASPXW010000070         3085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1662017, whole genome shotgun sequence.
ACCESSION   DASPXW010000070 DASPXW010000000
VERSION     DASPXW010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1662017"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1564)
                     /gene="recJ"
                     /locus_tag="VEL04_01645"
     CDS             complement(<1..1564)
                     /gene="recJ"
                     /locus_tag="VEL04_01645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HYT47100.1"
                     /translation="MISPRWILTATADPDATQRLASELRIPDALAAILVQRGLASPEL
                     AKAFLRPDLERLSDPHSWADMPAALELLERAVHEKRPILVHGDYDVDGQCASAMLTRM
                     LRSAGATVHPFVPNRIRDGYDFGPAGLAEAQRVGARLIVTCDCGITAVQAVAAARAAG
                     IDVIVTDHHLPGDELPPANAVLDPRRPDCKSEDKNLCGTGVAFKLAQALVEVLGLSHN
                     LPLHFLDYVALATIADVVPLVGENRILVRYGLKKLADSRWVGLRALIETAGLGGKPLR
                     GAHVGFILAPRLNAAGRIGDANDGLRLLLTEDPKEAAKLARELETINARRQEMDQLIL
                     DEAIELVDKTLNPADAAIVLGAETWHPGVIGIVASRLVDRYGRPTFLIGWDEAGEFGR
                     GSGRSIPGFDLHGALHKVGTHLEKYGGHTMAAGFTIRRDKFDDFRAAFLGVAGELLTP
                     DDLAPSQRVDLELPLAEVSPDLERLIRHLEPCGAGNPAPVFGVRNARAVGARRVGTNH
                     LRFTLDDGSGLLP"
     gene            complement(1561..2265)
                     /locus_tag="VEL04_01650"
     CDS             complement(1561..2265)
                     /locus_tag="VEL04_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47101.1"
                     /translation="MHPDLEALLVLQDADVAVSSCDARLKALEPEVRRLDEQIAAAER
                     VVEQARAGIQAALDRRDGLEGKIQSYRTMQEQRRQRLEWVRGAKEASTLMAELDLARS
                     VLAKEEAEFMRSGDAVGEAERKTAEAENALQNVRAAQAAQREALAAKRQEMAAERESA
                     ALEREQASRGVNPALLARYDRIRRGKAPLAIYPLHGSSCGNCFTAVPTQRKALIQRGA
                     TIEGCEACGVLLYAKE"
     gene            complement(2249..>3085)
                     /locus_tag="VEL04_01655"
     CDS             complement(2249..>3085)
                     /locus_tag="VEL04_01655"
                     /inference="COORDINATES: protein motif:HMM:NF021895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47102.1"
                     /translation="GLRATFRAGDVLLRHKLRVSVASLPEGEDPDTLVQNKGAAALEQ
                     ALKDSVDVLERKIQILDRKGFFGSLPGRRRALDRLLPTIRAAADPITKELYISRVAEV
                     AGIRKDVLERETAGKGDWGMAPKTTHSPVPIPPMPAGAEKALLLLMLSGEPWKSRVQD
                     AVDSEEFAFEPYRLVFEALADDAPDRLDETAARAYESLQAEGLGREQPDALFERAVNQ
                     LEARRLDREIERINRALPFASDAEKATLAHEKVRLAAERNAKRPTYGIIQSARRKGAP
                     GS"
BASE COUNT          483 a         1084 c          998 g          520 t
ORIGIN      
        1 ccgggagcag gcccgagcca tcgtccaacg taaaccgtag gtgattcgtg cccacgcggc
       61 gcgcccccac cgcgcgcgcg ttgcggacgc cgaacaccgg cgccggattc cctgccccgc
      121 acggctccag gtgtcggatc aggcgttcca gatccgggct cacttcagcc agtggcaact
      181 cgagatccac gcgctgactc ggcgccaggt cgtcgggggt cagaagctcg cccgccacgc
      241 cgaggaacgc agcgcggaaa tcgtcgaact tgtcgcgccg aatcgtaaag ccggccgcca
      301 tcgtgtgacc gccgtacttc tcgaggtgcg tgccgacttt gtgcagtgcg ccatgcagat
      361 cgaagcccgg gatgctgcgc cccgagccac gcccgaactc ccccgcttca tcccaaccga
      421 tcaggaacgt cggccggccg tagcggtcca cgagccgcga ggcgacgatc ccgatgacgc
      481 ccggatgcca cgtctcggcg ccgagcacga tggcggcgtc ggcgggattc agcgtcttgt
      541 ccacgagctc gatcgcctcg tcgagaatca gctgatccat ctcctggcgg cgagcgttga
      601 tcgtctcgag ctcgcgcgcg agcttcgccg cttccttcgg gtcttccgtg agcagcaggc
      661 gcagaccgtc gttcgcgtcg ccgatgcggc ccgcggcgtt gaggcgcggc gcgaggatga
      721 accccacgtg cgccccgcgc agcggcttgc cgccgagccc ggcggtctcg atcagggccc
      781 gcagcccgac ccagcgcgag tcggcgagct tcttgagccc gtagcgcacc aggatgcggt
      841 tctcacccac gagcgggacg acgtcggcga tcgtcgcgag ggcgacgtaa tcgaggaaat
      901 gcagcggcag attgtgcgac aggccgagca cctcgaccag cgcctgcgcg agcttgaagg
      961 cgacaccggt gccgcagagg ttcttgtcct ccgacttgca gtcggggcgg cgcgggtcga
     1021 ggacggcgtt cgcgggcggc agctcgtccc ccggcaggtg gtgatcggtg acgatcacgt
     1081 cgattcccgc ggcgcgcgcg gcggcaacgg cctgcacggc ggtgatgccg cagtcgcagg
     1141 tgacgatcaa ccgcgcgccc acgcgctgcg cctccgccag gcccgccggc ccgaagtcgt
     1201 agccgtcacg gatgcgattg ggcacgaacg gatgcacggt cgcgcccgcg ctgcgcagca
     1261 tgcgcgtgag catcgccgag gcgcactggc cgtcgacgtc gtaatcgccg tgcaccagga
     1321 tcggccgctt ctcgtggacc gcgcgctcca gcagctccag cgcggccggc atgtcggccc
     1381 aggagtgcgg gtcggacagg cgctcgaggt cgggacgaag gaacgccttg gcgagctccg
     1441 gcgacgctag tccgcgctgt acgagaatgg cggcgagggc gtcggggatg cgcagctcgg
     1501 acgcgagccg ttgggtcgcg tcgggatcag ccgtggctgt gaggatccag cgaggtgaga
     1561 tcattccttg gcgtacaata atacgccgca ggcttcgcag ccttcgatcg tggcgccgcg
     1621 ctggatcagt gccttgcgct gcgtcgggac cgccgtgaag cagttcccac acgagctgcc
     1681 gtgcaacgga tagatcgcca gcggcgcctt gccgcgccga atgcgatcgt aacgcgccag
     1741 cagcgcaggg ttcacaccgc ggctcgcctg ttcgcgctcg agcgccgcgc tctcgcgttc
     1801 ggctgccatc tcttggcgct tggccgcgag cgcctcgcgc tgcgccgcct gcgcagcgcg
     1861 cacattctgg agcgcgttct cggcttcggc ggtcttgcgc tccgcctccc ccaccgcgtc
     1921 acccgagcgc atgaactcgg cttcttcctt cgcaagcacg gaacgggcga ggtcgagctc
     1981 cgccatcaac gtggacgctt ctttcgcgcc gcgcacccac tcgagccgct ggcggcgttg
     2041 ctcctgcatc gtgcgataac tctggatctt cccttccagt ccgtcgcgcc gatcgagtgc
     2101 cgcctggatc cccgcgcgcg cctgctccac cacccgctcg gccgcggcga tctgctcgtc
     2161 cagccgcctg acctccggct ccagcgcctt cagccgggcg tcgcagctgg acaccgcgac
     2221 gtccgcatct tgtagaacca acaacgcttc aagatccggg tgcacccttc ctccttgcag
     2281 actgaatgat tccataggtc ggtcgcttgg cattacgctc agcggcgagg cggacttttt
     2341 catgcgcgag ggtcgctttc tcagcatcgc tcgcaaacgg tagcgcgcga ttgatacgct
     2401 cgatttcccg atccaggcgc cgggcttcaa gctggttcac ggcgcgctcg aatagcgcat
     2461 ccggttgctc gcgaccgagc ccttccgctt gtaacgattc gtaggcgcgg gcagcagttt
     2521 cgtcgagccg gtcgggagca tcatcggcga gggcttcaaa gaccaaccga tacggctcga
     2581 atgcaaactc ctcgctatcc acggcatcct ggacacgtga cttccacggc tcgcccgaca
     2641 acatcaggag caggagcgct ttttcggcgc ctgccggcat tgggggaatg gggactgggg
     2701 aatgggtggt cttcggtgcc attccccagt cccctttccc cgccgtttct ctctccagca
     2761 catccttcct tatccccgcc acctctgcga cgcgcgatat gtagagttcc tttgtgatgg
     2821 gatcagccgc cgcacgaatc gtcggcaata accgatcaag ggcacgccga cgccccggca
     2881 aacttccaaa aaatcccttg cgatcgagga tctggatctt gcgctcgaga acatccacgg
     2941 agtccttgag cgcctgctcg agggccgccg cgcccttgtt ctgaaccagc gtatcagggt
     3001 cctcgccctc gggcagcgag gcgacgctga cccgcagctt gtgacgcagc aacacatcgc
     3061 cggcgcggaa agtggcgcga aggcc
//
LOCUS       DASPXW010000071         2882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_529002, whole genome shotgun sequence.
ACCESSION   DASPXW010000071 DASPXW010000000
VERSION     DASPXW010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529002"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1194
                     /locus_tag="VEL04_01660"
     CDS             <1..1194
                     /locus_tag="VEL04_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HYT47103.1"
                     /translation="DSSGLAKAVEMCNNNGHCRKFDAGTMCPSYRATLDEAHLTRGRA
                     NTLRLALSGQLGPEADFMVNEALDLCVSCKGCKRECPTGVDMARMKIEHLAHYKAKHG
                     WTARDRAIAYLPRYAPWAASWAPVANLLSSLTKSLIGFSAKRDLPKWRRDIFDEVGST
                     DCDVVLFVDTFNRYFEPENARAALKVLQAAGNRVYAPQGLCCGRTFLSVGLVDEAKRE
                     ARKLLHALAPYAERAIPVVGLEPSCLYSLRDEFPVLLPGADKLAKSALLLEEFLARNP
                     GRLTFKETNRQVLLHGHCHQKAFDAMPAVEKVLRSIPGIGLKTIQASCCGMAGSFGYE
                     AEHYDVSMKMAEASVLPEIRKSPDAIVVADGTSCRHQIADGTGRVAWHVARVLEMALA
                     STPSE"
     gene            complement(1171..1941)
                     /gene="lgt"
                     /locus_tag="VEL04_01665"
     CDS             complement(1171..1941)
                     /gene="lgt"
                     /locus_tag="VEL04_01665"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HYT47104.1"
                     /translation="MLVHPDIDPIAFAIGPVAVRWYGLMYLAGFAAGWWLGMSRIKRG
                     AAPVSRIQFDDLLFLVVLGVILGGRLGYVLLYKPGYYASDPLEILALWRGGMSFHGGL
                     LGVMIAMLFAARRHGVDYLRLMDFVVPLVPPGIAAGRLGNFINGELWGRVTDLPWGMV
                     FRGAGDAPRHPSQLYQFALEGLALFALLWWFSSRPRPRGQVSAVFLIGYGAFRFLAEF
                     AREPDAFLGTLAFGMTMGQWLCVPMIAGGAWLFAWSRR"
     gene            complement(1951..2847)
                     /locus_tag="VEL04_01670"
     CDS             complement(1951..2847)
                     /locus_tag="VEL04_01670"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HYT47105.1"
                     /translation="MNLPEMWQLRYFIAVAECLHFGRAAAALHISQPPLSRAIRALED
                     RVGVALFTRSRRRVELTPEGLRFLGAARRVADQLGRAVSELRGMASGEHGRLRIGFVS
                     LADYGMLPGLLKAYKAARPGVALALREMLSPEQAAALAAGELDFGLLLPPVRGTSGLD
                     HVVVQRERFVAALPSRHRLASARGGLAMSALAGESFVMVPREIAPGLYDIVTGLATRA
                     GFSLNIAQEAIQMQTIVSLVSSGLGAAIVPASIANLGRRGVVYRELADRHPALELWLA
                     WCRGALGAAGREFVAHARRIAP"
BASE COUNT          550 a          942 c          969 g          421 t
ORIGIN      
        1 gactcgagcg ggctcgcgaa ggcggtcgag atgtgtaaca acaacggcca ctgccgcaaa
       61 ttcgacgccg gcaccatgtg cccatcgtat cgggcaacgc tggacgaagc gcacctcacg
      121 cgtgggcgcg ctaacacgct gcgcctggca ctttcaggcc aattgggccc ggaggccgat
      181 ttcatggtca acgaagccct ggacttgtgc gtgtcatgca agggatgcaa gcgcgagtgc
      241 ccgactggtg tcgacatggc gcgcatgaag atcgaacacc tcgcgcacta caaggcgaaa
      301 catggctgga cagcgcgcga tcgcgcgatc gcctatttgc cgcgctacgc gccttgggcg
      361 gcgagttggg cgccggttgc caatctgctc tcctctttaa cgaaatcttt aatcggtttt
      421 tcagccaaac gcgacctgcc gaaatggcgc cgcgacatct ttgacgaggt gggcagcacc
      481 gactgcgacg tggtgctttt cgtcgacacc ttcaatcgtt atttcgagcc cgagaacgcg
      541 cgcgccgcgc tgaaggtgct gcaggccgcc ggcaatcgag tctatgcgcc gcaaggcctg
      601 tgctgcgggc gtacgtttct ttccgtcggg ctggtcgatg aggcgaagcg cgaggcgcgc
      661 aagctgctcc acgcgcttgc gccgtacgcg gagcgcgcaa ttccggtggt cgggctcgaa
      721 ccctcgtgcc tctactcgct gcgcgacgag ttccccgtgt tgctgccggg cgccgacaag
      781 ctcgcgaaga gcgcgttgct gcttgaggaa ttcctcgccc gcaatccggg acgattgaca
      841 ttcaaagaaa caaacagaca ggttctcttg cacggccact gccatcagaa ggcgttcgac
      901 gccatgcctg cagtggaaaa ggtattgcgc tcaattccag gcatcgggct gaagacaatc
      961 caggcgagct gctgcgggat ggccgggagc ttcggctatg aggccgagca ttacgacgta
     1021 tcgatgaaga tggccgaggc gagcgtgctt ccggaaatca ggaaatctcc ggatgcgatc
     1081 gtggttgccg acggcacgag ctgccggcat cagattgcgg acggcaccgg ccgggtcgcc
     1141 tggcacgtgg cgcgcgtgct ggagatggcg ctagcgtcga ctccaagcga atagccacgc
     1201 cccgcctgcg atcatcggca cgcacagcca ttggcccatg gtcatgccga acgcgagcgt
     1261 accgaggaag gcgtcgggct cccgggcgaa ttccgcgagg aagcggaatg ccccgtagcc
     1321 gatgagaaac acggccgaca cctggccgcg cgggcgtggc ctggaggaaa accaccacag
     1381 cagcgcgaac aacgcgaggc cctcgagggc gaactggtag agttgcgaag ggtgccgggg
     1441 cgcatcgccc gcgccgcgga ataccatgcc ccatggcaga tcggtgacgc ggccccagag
     1501 ctcgccgttg atgaagttcc cgaggcgccc ggccgcaatg ccgggcggca ctagcggcac
     1561 gacgaagtcc atcaggcgca gataatcgac gccgtggcgc cgggccgcga acagcatcgc
     1621 aatcattacc cccagcagcc cgccatggaa cgacatgccg cctcgccaca gcgcgaggat
     1681 ctcgagcgga tccgaagcgt agtaccccgg cttgtagagc agcacgtagc cgagccggcc
     1741 gcccaggatc acgccgagca cgacgagaaa cagcagatcg tcgaactgga tgcgcgacac
     1801 cggtgcggcg ccgcgcttga tgcggctcat gccgagccac cagccggcag cgaacccagc
     1861 caggtacatc agaccgtacc agcgtacagc gaccgggccg atcgcgaagg cgatcgggtc
     1921 gatatcggga tgcaccagca tggccgggtg ctatggcgcg atgcgtcgcg cgtgcgccac
     1981 aaattcgcgg cctgccgcgc cgagcgcacc gcgacaccag gcgagccaca gctccagcgc
     2041 cgggtgcctg tcggcgagct cgcggtacac cactccgcgc ctgccgaggt tggcgatcga
     2101 ggccgggacg atcgctgcgc ccagtccgct cgacaccagg ctcactattg tctgcatctg
     2161 tattgcctct tgggcgatgt tcagggagaa gcccgcccgc gtggcgaggc cggtcacgat
     2221 atcgtacagg ccgggcgcga tctcgcgcgg caccatgacg aagctttcgc cggcgagcgc
     2281 gctcatggcg agaccacctc gcgcggatgc gaggcgatgc cgggagggga gcgcggcgac
     2341 gaaccgctca cgctgcacga ccacatggtc caggccggag gtgccacgca ctggcggcag
     2401 cagcaggccg aagtcgagct cgcctgccgc gagggcagcc gcctgctccg gagagagcat
     2461 ctcccgcaat gcgagcgcaa cgccgggccg ggcggctttg taggccttca gcagcccggg
     2521 aagcatgccg tagtcggcca gcgatacgaa gccgatgcgc agccgcccgt gctcgccgga
     2581 ggccatgccg cgcagctcgc tcaccgcgcg cccgagctgg tccgccacgc gtcgcgccgc
     2641 gccgaggaag cgcaggccct cgggggtgag ctcgacgcgc cggcgggagc gcgtgaacaa
     2701 ggctacgccg acccggtcct ccagggcacg gatggcgcgc gacagcggcg gctgcgagat
     2761 gtgcagtgcc gcggcggcgc ggccgaagtg caggcactcg gcgacggcga tgaaataccg
     2821 caactgccac atttcaggca gattcatacc catagagtat tacagcagga cccagattgc
     2881 at
//
LOCUS       DASPXW010000072         3636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_905903, whole genome shotgun sequence.
ACCESSION   DASPXW010000072 DASPXW010000000
VERSION     DASPXW010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905903"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VEL04_01675"
     CDS             <1..397
                     /locus_tag="VEL04_01675"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HYT47106.1"
                     /translation="SFKPAGTLMLVPASVNGVEATLLLDTGASVTLNRPALASRAGVE
                     VPARARRMYVRVLGGERFLVPFVKVQSLRVGDAAVEGIDVGVFEGLGSFPRSVDGVLG
                     GNFLNHFKVTIDRQGRRLTLEPSRTAAAQ"
     gene            417..797
                     /locus_tag="VEL04_01680"
     CDS             417..797
                     /locus_tag="VEL04_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011659979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HYT47107.1"
                     /translation="MVVHAGGCHCGRVRFEVTAPAAISVAECNCSICSKSGYLHLTVP
                     KSRFRLLQGEDFLTRYGFNTGAARHLFCKVCGIKSFYVPRSDPEGISVNARCLDPGTV
                     TRMELRPFDGQNWEASIKGKSWQG"
     gene            785..1009
                     /locus_tag="VEL04_01685"
     CDS             785..1009
                     /locus_tag="VEL04_01685"
                     /inference="COORDINATES: protein motif:HMM:NF018369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HYT47108.1"
                     /translation="MARLIIAALVLGLAGTAWAQRKDCDELKGEIEAKIKKNGVDKFT
                     LDIVDADKQAEGKVVGTCDGGTKKIVYKRG"
     gene            complement(1006..2202)
                     /locus_tag="VEL04_01690"
     CDS             complement(1006..2202)
                     /locus_tag="VEL04_01690"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYT47109.1"
                     /translation="MLVVFCPFAAGYYLSYFYRYVNAVIARDLVSDFGLAPGDLGWLT
                     SAYFLSFALAQVPLGVALDRFGPRRCVAALMCVAAAGALTFGLARDLAMLSSGRALIG
                     LGVSAGLMGSIKAFTLWFPRERLTALNGWMIGIGSIGALSATAPVQALLGPFGWRALF
                     IALALLSLAAALLIFFVVPERELPGRGETWSEQFRTIAKIFSRVDFWRLAAPLVLCQA
                     SFQALQGLWFAPWLADVHGFSRATAANYLFASALAYLVASALLGQLTELLAKRGVSEL
                     RLYQAGMLACAASFAPIALGAASGTLLWLVLFAATSIAAITAYTLLTQLVPTAQTGRV
                     TTSSNVLLFATSFVFQWGVGTLLGLWPSSEGRYQPEAYRAAFGMLLALQAGAALWLLT
                     ARQETR"
     gene            2249..2839
                     /locus_tag="VEL04_01695"
     CDS             2249..2839
                     /locus_tag="VEL04_01695"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HYT47110.1"
                     /translation="MKKRLIVGITGATGAVYGIHLLEVLKGLKDWESHLVLTDAGLLN
                     LWHELKMKRKDAERLADVAYHPKDIAATIASGSFLTAGMVIAPCSMKTLAAVAHAHAD
                     DLVSRAADVVLKERRRLVLMPRETPLNLAHLRNMVAVSEMGAIVFPPVPAFYAAPKSL
                     DDLVAHSVARVLDLFGVHSARLRRWQGLKGTPAERG"
     gene            complement(2811..3578)
                     /gene="pcaD"
                     /locus_tag="VEL04_01700"
     CDS             complement(2811..3578)
                     /gene="pcaD"
                     /locus_tag="VEL04_01700"
                     /EC_number="3.1.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate enol-lactonase"
                     /protein_id="HYT47111.1"
                     /translation="MRATVNGNETNYEIHGKEGAPWLTFSHSLACSVRMWDGQIAAFK
                     ERWHILVYDTRGHGASTAPAGPYALETLVEDLHALLKHLKIARTRFVGLSMGGMIGQL
                     FALGHPGIFERLVLADTGHAQTPETQKQWQERIQTAQAKGMQALVQPTLERWFTEPFR
                     KSEPARKIGELIAKTPVAGYVGCCQAISKLSTTARLQDIKLPVLALAGEQDVAAAGTR
                     YIGEHVPGAKLVMIPQAAHIANVEQPEAFNRALREFL"
BASE COUNT          653 a         1143 c         1274 g          566 t
ORIGIN      
        1 ctcgttcaaa ccggccggaa ccttgatgct ggtaccggct tccgtcaacg gcgtcgaggc
       61 gacgttattg ctcgacaccg gggcgagcgt caccctcaac cggccggcgc tggcaagccg
      121 ggcaggggtc gaagtgcctg cccgcgcgcg gagaatgtac gtgcgcgtat tgggcggcga
      181 gcgattcctg gtgcccttcg tgaaagtgca gtcgctacgg gttggcgacg cagccgtgga
      241 aggcatcgac gtcggggtct tcgaggggct ggggtcgttt ccgaggagcg tggacggtgt
      301 gctgggcggc aacttcctaa atcacttcaa ggtgacgatt gacagacagg gccgccggct
      361 cacgctcgaa ccgtcgcgca cggccgcggc gcagtgactc gcgggcgtcg ccgataatgg
      421 tggtgcacgc aggcggctgc cattgcggcc gcgtgcgctt cgaggtgacg gcgccggcgg
      481 caatcagcgt cgcagagtgc aactgctcga tctgcagcaa atccggctac ctgcacctga
      541 ccgtcccgaa atcgcgcttt cgcctgctcc agggcgaaga cttcctgacc cggtacggtt
      601 tcaataccgg cgccgcccgg cacctttttt gcaaagtgtg cggaatcaag tcattctatg
      661 tgccgcggtc ggacccggag gggatcagcg tcaacgcgcg ctgcctcgat ccggggacgg
      721 tgacgcgcat ggagctgcgg cctttcgacg ggcagaactg ggaagcttct ataaagggaa
      781 aatcatggca aggctgatca ttgcggcact ggtgctggga cttgcgggaa ccgcctgggc
      841 gcagaggaag gactgcgacg agctcaaggg cgagatcgag gccaagatca agaagaacgg
      901 cgtcgacaag ttcacgctcg acatcgtcga tgccgacaag caggccgagg gcaaggtggt
      961 cggcacctgc gacggcggca cgaagaagat cgtctacaag cgcggctagc gcgtctcctg
     1021 ccgagccgtc agcagccaca aggctgcgcc ggcttgcaga gcgagcagca tcccgaacgc
     1081 ggcgcggtag gcctcgggct ggtagcgtcc ttcggagctt ggccacaggc ccagcagggt
     1141 gccgacgccc cactggaaga cgaaggacgt cgcaaaaagg agcacgttcg aggacgtggt
     1201 gacgcgcccg gtctgcgctg tgggcacgag ctgcgtgagg agcgtgtaag ccgttatcgc
     1261 ggcgatgctc gtcgcggcga agagcacgag ccagagaagc gtgccgctcg ccgcgccgag
     1321 cgcaatcggg gcgaagcttg ccgcgcaggc gagcatccca gcctggtaga ggcgaagctc
     1381 cgatacgccg cgcttcgcca gcagttcggt tagctggccg agcagtgccg aagcgacgag
     1441 gtaagcgagc gccgaggcga agaggtaatt ggctgccgtt gcgcgactga agccgtggac
     1501 gtcggcgagc caaggcgcaa accacaagcc ctgcagcgct tggaagctcg cctggcaaag
     1561 cacaagcggc gcggcgaggc gccagaagtc cacgcgcgag aaaatcttcg cgatcgtgcg
     1621 gaactgctcg ctccaggtct cgccgcggcc gggaagctcg cgctcgggga cgacgaaaaa
     1681 aatgagcagc gccgcggcga ggctcaagag agcgagcgca atgaacaacg cccgccagcc
     1741 gaaaggcccg agcagcgcct gcaccggcgc ggtggccgac agcgcgccga tgctgccgat
     1801 gccgatcatc cagccgttga gcgccgtcag ccgctcgcgc gggaaccaca gcgtgaaagc
     1861 cttgatcgag cccatcaggc cggccgagac gcccaggccg atcagcgcgc ggccgctcga
     1921 gagcatcgcg agatcgcgcg cgaggccgaa ggtgagagcc cccgcggctg cgacgcacat
     1981 cagggccgcg acgcagcgcc gcgggccgaa gcggtcgaga gccacgccga gcggcacctg
     2041 ggcgagcgcg aacgaaagga agtacgcgct cgtcagccag cccagatcgc ctggcgcgag
     2101 gccgaagtcg ctcaccaggt cacgcgcgat gacggcgttg acgtagcggt agaagtagga
     2161 gaggtaatac ccggccgcga acgggcagaa caccacaagc atcaaccgag cttcccagcg
     2221 agccatttag gcaggtaggc tacgggccat gaagaagcga ctgatcgtcg gcatcaccgg
     2281 cgccaccggc gcggtgtacg gcattcacct gctcgaagtc ctgaaagggc tcaaggactg
     2341 ggaaagccat ctcgtgctca cggacgcagg cttgctcaac ctgtggcatg agctgaagat
     2401 gaagcgcaag gacgccgagc gcctcgccga cgtcgcctac cacccgaagg acatcgcggc
     2461 gacgatcgcg agcggctcgt tcctcactgc ggggatggtg atcgcgcctt gctcgatgaa
     2521 gacgctcgcg gcggtggcgc acgcgcacgc cgacgatctg gtgtcgcgcg ccgccgacgt
     2581 ggtgctgaag gagcgccggc gcctcgtgct gatgccgcgc gagacgccgc tcaacctcgc
     2641 ccacctgcgc aacatggtgg cggtgagcga gatgggcgcg atcgtgttcc cgccggtgcc
     2701 cgcgttctac gccgcgccga agtcgctcga tgatctggtc gcgcactcgg tggcgcgggt
     2761 gctcgatctg ttcggcgttc actccgccag gctcaggcgc tggcaggggc tcaaaggaac
     2821 tcccgcagag cgcggttgaa ggcctcgggc tgctcgacgt ttgcgatgtg cgcggcctgc
     2881 ggaatcatca ctaatttcgc ccccggcacg tgctcgccga tgtagcgcgt gcccgccgcg
     2941 gcgacgtcct gctcgccggc aagcgcgagc accggcagct tgatgtcctg cagccgcgcg
     3001 gtggtgctga gcttcgagat cgcctggcag cagccgacat agcctgcgac cggggttttc
     3061 gcgatgagct cgccgatttt cctcgccggc tcgcttttcc tgaaaggttc ggtgaaccat
     3121 cgttcgaggg tgggctgaac gagcgcctgc atgcccttcg cctgcgcggt ctggatgcgc
     3181 tcctgccact gcttctgggt ttcaggcgtt tgcgcatgac cggtgtcggc cagcaccagg
     3241 cgctcgaaga tgcccggatg gccgagcgcg aacagctggc cgatcatgcc gcccatcgac
     3301 aggccgacga accgtgtgcg cgcgattttc aggtgcttga gcagggcgtg gaggtcctcg
     3361 accagggtct cgagggcgta ggggcccgcc ggcgcggtgc tcgcgccgtg gccgcgcgtg
     3421 tcgtagacca agatatgcca gcgctccttg aacgcggcga tctgcccgtc ccacatgcgc
     3481 acgctgcagg cgagcgagtg gctgaaggtg agccagggcg cgccttcctt gccgtgaatt
     3541 tcgtagttgg tctcgttgcc gttgacggtc gctctcatgt ttgctccatg atcaggggga
     3601 tcagcatttc atcttcgctg gtgccgccgt ggttgc
//
LOCUS       DASPXW010000073         4045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_780412, whole genome shotgun sequence.
ACCESSION   DASPXW010000073 DASPXW010000000
VERSION     DASPXW010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780412"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VEL04_01705"
     CDS             complement(<1..186)
                     /locus_tag="VEL04_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine phosphorylase"
                     /protein_id="HYT47112.1"
                     /translation="MLAVIGGSGVYNVDGLRNPRWVKAESSFGSPSDEILVGELEGLE
                     VAFLPRHGRGHRLPPGEI"
     gene            240..1181
                     /locus_tag="VEL04_01710"
     CDS             240..1181
                     /locus_tag="VEL04_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004544211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYT47113.1"
                     /translation="MNRLAGVWMDDYTYGAPIGRPIISSALEAPDVIPYAPDYQFQRR
                     VRVLVTGISGFIGSTLEPALAERGHEVRGVTRQTVSLKDAFAGCDAVVHLANLAHAPA
                     NRSLLWRVNVEGTRRAAELASAAGVRRFVYLSSIKASAPDDDYARAKLAAEQAVAGVA
                     ARSGLQAVILRPPLVYGPRVKANFLALMRAIDRGWPLPFARIENRRSLLYVGNLCDAI
                     ARCLEAEAPDGRTFCVADGAPLSTPQLCHAIGNALGRPARLFPFPVPLLELIPALKKL
                     TRSLEADDAAIRRELDWRAPYSFEQGLLATADWYRSR"
     gene            1192..2163
                     /locus_tag="VEL04_01715"
     CDS             1192..2163
                     /locus_tag="VEL04_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYT47114.1"
                     /translation="MLGAVVSFVVAFVAVRVLLSRFGRFALDEPNARSLHERPVPRTG
                     GIAVLLGVAVSLGFGALQLWLPMALALVLAIVSFLDDVRGMPTVARLVIHLAAAATLV
                     WYVLSPMHPLELALLIFAVAWITNLYNFMDGSDGLAAGMAIIGFGAYALAAWIAGHDS
                     LAVLTVSITAASTAFLLHNFHPARIFLGDVGSIPLGFLAAALGIVGWRDDVWPLWFPL
                     LAFGPFIGDASLTLLKRLVRRDRIWQAHREHYYQRMVRMGLGHRATAWTGYAVMAACA
                     GAALLGRDQPPALQAAAFAAASLLLAALAVWVDLRWARFLRGTQEAA"
     gene            2160..>4045
                     /locus_tag="VEL04_01720"
     CDS             2160..>4045
                     /locus_tag="VEL04_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007736750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HYT47115.1"
                     /translation="MNVRSLIVFLHDVTAAGLAWLASFWLRFNLDIPGEYARLMLDLL
                     PGVLVIHAAAFWALGLYRGLWRFASLPDLRRILIAVGIAALAVPTLMALLRVGSPVPR
                     TVYLLTPLLLILIMSGSRIAYRAGKEGHFAAMLAKPQSTPVIVIGAGSAGAALLKDLA
                     ASRTWRVLGVLDDDVGKQGGEILGVKVLGRLERIGNAAEALGVTQAIIAMPEATHGER
                     KRAVELCTAAGLSVMTVPALSDIVSGKVSVSALRAIELDDLLGRDPVQLDEAGLHTFL
                     DGKSVLVTGAGGSIGAELCRQIARFRPARIVLLENSEFALYSIEQEFRDQSPGVPIAA
                     AIGDAKDERRVNEVFARHRPAVVFHAAAYKHVPLMEDENCFQAVGNNVLSTVVVARAA
                     ESAGAEKLVLVSTDKAVNPANVMGASKRLAEMACQSLQAASRMQFVMVRFGNVIGSNG
                     SVVPRFREQIARGGPVTVTHPEIQRYFMSIPEAAQLVLQAAMMGRGGEIYVLEMGEAV
                     RIADLARQMIRLSGFGENDIRIEYTGLRPGEKLYEEPLADAERTLATPHPKLRVAQAR
                     VPQNGRFLDEVLQWLERPDDTSASGVRVRLRGWVPEYQPPIAAAPTASLSPGASGGGA
                     ETPR"
BASE COUNT          554 a         1351 c         1404 g          736 t
ORIGIN      
        1 gatctcgccg ggagggaggc gatggccgcg gccgtggcgc ggcagaaatg cgacctccag
       61 tccctccaat tcgccgacga gtatttcgtc cgacgggctg ccgaacgaag actcggcttt
      121 cacccagcgc gggttgcgca ggccgtctac gttatagacc ccgctgccgc cgatgaccgc
      181 gagcatttct tttcctcgcc tcttttgttc gatcgggccc tgggcgccgc gcgaatgcgg
      241 tgaaccgcct ggcgggtgtc tggatggatg actatacata cggtgctccc attggcaggc
      301 cgattatatc gagcgcgctc gaggcgcccg atgtaatacc atacgcgccc gattatcaat
      361 ttcagcgccg ggtgcgcgtc ctcgtcaccg gaatttccgg attcatcggc agcacgctcg
      421 agcccgccct cgccgagcgc gggcacgagg tgcgcggcgt gacgcgccag acggtttcgc
      481 tgaaggatgc cttcgccggc tgcgatgcgg tggtccatct cgcgaatctc gcgcacgcgc
      541 ctgctaatcg gtcgttgctg tggcgggtga acgtcgaagg aacgcgccgg gctgctgagc
      601 tcgcgagcgc cgcaggcgtg cgccggtttg tgtatctcag ctcgatcaag gcgagcgccc
      661 cggacgacga ttacgcacgc gccaagctcg ccgccgagca ggccgtagcg ggcgtcgccg
      721 cgcgctcggg cctgcaggcg gtgatcctgc ggccgccgct cgtgtatggg ccgcgagtga
      781 aggccaactt tcttgccctg atgcgcgcga tcgaccgcgg ctggccgctg ccctttgccc
      841 gcatcgagaa ccgccgcagc ctgctctatg tcggaaacct ctgcgacgcc attgcgcgct
      901 gtctcgaggc cgaggcgccc gacgggcgga cgttctgcgt cgccgacggc gcgccgctct
      961 cgacgccgca gctttgccac gccatcggca atgcgctcgg ccggccggcg cgtctttttc
     1021 cttttccggt gccgttgctg gaattgatcc ccgcgttgaa aaagttgacg cgttcgctgg
     1081 aggcggacga cgccgccatc cggcgcgagc tcgactggcg agcaccttac tcgttcgagc
     1141 agggattgct tgcaacggcc gattggtacc ggtcccgcta gaatgccgcc gatgctcggg
     1201 gcggtcgtgt cattcgtggt tgcgttcgtt gcggtgcgcg ttctgctgtc gcgcttcggc
     1261 cgcttcgcgc tcgatgagcc gaacgcacgc tcgctgcacg agcgcccggt gccgcgcacc
     1321 ggcggcatcg cggtattgct cggcgtcgcg gtttccctgg gatttggcgc cctgcagctg
     1381 tggctgccga tggcgctggc gttggtcctg gccatcgttt cgttcctcga cgatgtgcgc
     1441 ggcatgccga ccgtggcgcg gctcgtgatc catctcgcgg ccgcggccac cctggtgtgg
     1501 tacgtcctga gcccgatgca tccgctcgag ctcgcgcttc tgatcttcgc ggtggcatgg
     1561 atcaccaacc tctacaactt catggatggc tccgacggcc tcgccgcggg catggcgatc
     1621 atcggcttcg gcgcctacgc gctcgccgca tggatcgccg gccatgactc gcttgcagtg
     1681 ttgacggttt cgatcactgc ggcatcgacg gcctttctgc tgcataactt tcacccggcg
     1741 cggattttcc tcggcgacgt cggctcgatc ccgctcggct ttctcgccgc ggcgctcggc
     1801 atcgtcggct ggcgcgacga cgtctggccg ctctggtttc cgctgctcgc cttcgggccg
     1861 ttcatcggcg acgcgagcct cacgctgctg aagcgccttg tacggcgcga tcgcatctgg
     1921 caggcgcacc gcgagcacta ctaccagcgc atggtgcgca tgggccttgg ccaccgcgcc
     1981 accgcctgga ccggctacgc ggtgatggcg gcctgcgcgg gcgccgcgct tctgggacgc
     2041 gatcagccgc cggcgctgca ggccgcggcg ttcgcggctg ccagcctgct gctcgcggcg
     2101 ctggcggtgt gggtcgacct gcgctgggcg cgctttctgc gcggcacgca agaagccgca
     2161 tgaacgtgcg cagcctgatc gtctttctgc atgacgtaac cgccgcgggg ctggcgtggc
     2221 tggcgagctt ctggctgcgt ttcaacctcg acatccccgg cgagtacgcg aggctgatgc
     2281 tcgacctgct gccgggagta ttggtgatcc atgcggctgc gttctgggcg ctcggcctgt
     2341 accgcgggct gtggcgcttc gccagccttc ccgacctgcg gcgcattctc atcgcggtgg
     2401 ggatcgcggc gctggcagtg cccacgctca tggcgttgct gcgggttgga tcgccggtgc
     2461 cgcgcaccgt gtacctgctg acgccgctgc tgctgatatt gatcatgagc ggcagccgta
     2521 tcgcctatcg cgccgggaag gaggggcatt tcgcggcgat gctcgccaag ccgcagtcga
     2581 cgccggtaat cgtgataggg gcaggctcgg ccggcgcggc gctgctgaag gacctcgcag
     2641 cgagccgcac ctggcgcgtg ctgggggtgc tggatgacga cgtcggcaag cagggcggcg
     2701 aaatcctcgg cgtcaaggtg ctcggccgcc tcgagcgcat aggcaatgcc gccgaggcgc
     2761 tcggcgttac gcaggcgatc attgccatgc ccgaggcgac gcacggcgag cgcaagcgcg
     2821 cggtggagct ttgcaccgcc gccgggcttt ccgtgatgac cgtgcccgcg ctgtcggaca
     2881 tcgtgtcggg gaaggtgagc gtctccgcat tgcgcgcgat cgagctcgat gacttgctcg
     2941 gccgcgaccc ggtgcagctc gacgaggccg ggctgcatac ctttctcgac ggcaagtcgg
     3001 tccttgtgac cggcgctggc ggatcgatcg gagcggagct gtgccggcag atcgcgcgtt
     3061 tccggccggc acgcatcgtg ctcctcgaga actcggaatt cgccctttac tccatcgagc
     3121 aggagttccg cgaccagtct ccgggagtgc cgatcgccgc ggcgatcggc gacgcgaagg
     3181 acgagcggcg cgtgaacgag gtgttcgccc gccaccgccc ggcggtggtt tttcatgccg
     3241 cggcctacaa gcacgtgccg ctcatggagg acgagaattg tttccaggcg gtgggaaaca
     3301 acgtgctttc cacggtcgtc gtggcgcgtg ccgcggaaag cgccggcgcg gaaaagctcg
     3361 tcctggtgtc gaccgacaag gcggtcaatc cggccaacgt catgggagcc tccaagcggc
     3421 tcgccgagat ggcctgccag tccttgcagg cggcgagccg catgcagttc gtcatggtgc
     3481 gcttcggcaa cgtgatcggc tcgaacggca gcgtggtacc gcgctttcgc gagcagatcg
     3541 cgcgcggcgg cccggtcacc gtgacgcacc ccgagatcca gcgctatttc atgtcgatcc
     3601 ccgaggcggc gcagctggtg ctgcaggccg ccatgatggg caggggcggc gagatctatg
     3661 tcctcgagat gggcgaggcg gtcaggatcg ccgacctcgc gcggcagatg atccgcctct
     3721 ccggcttcgg cgagaacgac atccgcatcg agtacacggg tctgcgcccc ggcgagaagc
     3781 tctacgagga accgctcgcc gacgccgaac gcacgctggc gacgccgcat cccaagctgc
     3841 gcgtcgcgca ggcgcgggtg ccgcaaaacg gccggtttct cgacgaggtg ctgcagtggc
     3901 tcgaacgtcc ggacgacacg agcgcaagcg gcgtgcgtgt gcggctgcgc ggctgggtac
     3961 cggaatacca gcctcccata gcggccgccc ctacagccag tctgtcaccg ggggcgtcgg
     4021 gcgggggagc ggagaccccg cggtg
//
LOCUS       DASPXW010000074         2869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1536492, whole genome shotgun sequence.
ACCESSION   DASPXW010000074 DASPXW010000000
VERSION     DASPXW010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1536492"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..1163
                     /gene="dprA"
                     /locus_tag="VEL04_01725"
     CDS             39..1163
                     /gene="dprA"
                     /locus_tag="VEL04_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HYT47116.1"
                     /translation="MTRDPGLASWLQLTLTPGLGNSAVRGMLRQFGLPQSILARKDTE
                     LSGFAVPAALEALRSEAVQRAVARALEWTEQPGNRLITLADETYPRMLLEIPDPPPVL
                     YARGRADLLQRPSLAIVGSRNATAQGAANAESFARSLSDSGLTIVSGLALGVDGAAHR
                     GGLAGASSTIAVLGTGIDVVYPRRNAELAAEIAARGLLVSEFGLGTEAVAHNFPRRNR
                     LISGLAQGCLVVEAALASGSLITARAAADQGREVFAIPGSIHSPLSKGCHALIKSGAK
                     LVESAEDVLAELAGFRASGYASTTLQPSSARGASAAPPSAFGLLEHMGHDPVDVDSLC
                     ARAGMSAEQVSSELLRLELAGRVTALPGGLFQRLEKGSGG"
     gene            1205..1663
                     /locus_tag="VEL04_01730"
     CDS             1205..1663
                     /locus_tag="VEL04_01730"
                     /inference="COORDINATES: protein motif:HMM:NF016264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HYT47117.1"
                     /translation="MYDILVYLFENCQQAELTYDRERVAKKLSAAGFEHSDISEALHW
                     LAGVLRAPQSGLDALPDSRRTFRAFAPRELAKLDAECRGFLLTLEHSGILTPQTRELV
                     IERSLAASGQYLTLEQLKLIVLMVLWNRQTPTSQLVAEDIFSAPHTRLPS"
     gene            1777..>2869
                     /locus_tag="VEL04_01735"
     CDS             1777..>2869
                     /locus_tag="VEL04_01735"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HYT47118.1"
                     /translation="MAKKLIIAEKPSVAADIARALGGFTRKGDYFESDRHVLSSAVGH
                     LLELAVPQDYEVKKGKWSFKHLPVIPPHFDLSPIERSQQRLNVLLRLIKRKDVTGVIN
                     ACDAGREGELIFRYLLQHARAKKPIERLWLQSMTQQSIRDGFAKLRPDKDLLPLADAA
                     KSRSEADWLVGINGTRAMTAFNSKEGGFYLTTVGRVQTPTLAILVEREDKIRAFTPRN
                     YWEVHARFGAKNGEYAGRWLNSGFKKDEDAERKPERLWNAAAAAKIVAACRGKKGIAT
                     EESKPTTQLSPLLFDLTSLQREANGRFGFPARMTLSLAQALYEKHKVLTYPRTDSRAL
                     PEDYLPTVKDALKALGETTAFSAFSRQILK"
BASE COUNT          501 a          979 c          902 g          487 t
ORIGIN      
        1 aaaagcgggg ccggcttgcc cgcttcgccg ccgccgccat gacgcgcgac cccgggctgg
       61 cgagctggct gcaactcacg ctcactcccg ggctcggcaa ctccgccgtc cgcggcatgt
      121 tgcgtcagtt cggtttgccg cagtcgattc tcgctcgaaa ggacaccgag ctcagtgggt
      181 tcgccgtgcc ggcggctctc gaggcattgc gttccgaggc ggtgcagcgc gcggtcgcac
      241 gtgcgcttga atggaccgag cagcccggca atcggctcat cacgctcgcc gatgaaactt
      301 atccgcggat gctgctcgag attcccgacc cgccgccggt actctacgca cgcggccgcg
      361 ccgatcttct gcagcgaccc tcgctcgcga tcgtcggcag ccgcaacgcc accgcccagg
      421 gggcagccaa cgcggagagc ttcgcgaggt cgctaagcga ctccggactc acgatcgtgt
      481 cggggcttgc tctcggtgtc gatggggccg cgcaccgtgg cggactcgct ggggcatcct
      541 cgaccatcgc ggtgctcggc accgggattg acgtcgtgta cccgagacgc aacgccgagc
      601 tcgccgccga aatcgcggcg cgcggcttgc tcgtgtcgga gttcgggctc ggcaccgagg
      661 cggtggcgca caattttccg aggcgcaatc gcctcatcag cgggttggcg cagggctgcc
      721 tggtcgtcga ggcggcgctc gcctcaggct cgctcatcac ggcgcgcgcc gccgccgatc
      781 agggccggga ggtgttcgcg atacccggct cgatccattc gccgctctcc aagggatgcc
      841 acgccctcat caaatcggga gcaaaacttg tcgagtccgc cgaagacgtg ctcgccgagc
      901 tcgccggctt tcgcgccagc ggctacgcct cgaccaccct gcagccgagt tccgctcgcg
      961 gcgcgagcgc cgcgccgccg agcgcattcg gtttgctgga acacatggga cacgacccag
     1021 tggacgtgga ctcgctttgc gcgcgcgccg gcatgtctgc cgaacaagta tcctccgaac
     1081 tgctgcggct cgaactcgcg ggtcgcgtga cggccctgcc aggcggccta ttccagcgcc
     1141 tggaaaaagg tagcggcggg taaacccgat acgggtcgcc gcgttataaa aagaaaggga
     1201 ctttatgtac gacatcctgg tctacctgtt cgagaactgc cagcaggcgg agttgaccta
     1261 tgaccgcgag cgtgtggcta agaaactttc cgccgccggc ttcgagcact cggacatcag
     1321 cgaggcgttg cactggctcg ccggcgtgct gcgcgcgccg cagagcgggc tcgacgcgct
     1381 gccggattct cgtaggacct tccgggcatt tgcgccgcgc gagctcgcga agctcgatgc
     1441 cgaatgtcgc ggtttcctgc tcacgctgga gcattctggc atcctgaccc cgcaaacccg
     1501 cgagctggta atcgagcgct cgctcgccgc ttcggggcaa tacctcacct tggagcagct
     1561 caaactgatc gtgctgatgg ttctctggaa ccggcagacc ccaaccagcc agctcgtggc
     1621 cgaagacatc ttcagcgcgc cgcacacgcg tctaccgagc taactcttcc ctcgcgtgcg
     1681 cgcgcccgcc cgcgcgcgca tagcaccgct tcacgggtgc tgcgtggttg aagccgcgca
     1741 gcgtttgcac ttatcatctg cggcctttcg cgccgcatgg caaaaaaact gatcatcgcc
     1801 gagaaacctt cggtcgccgc cgacatcgcg cgcgcgctcg gcggtttcac gcgcaagggc
     1861 gattacttcg aaagcgatcg ccatgtactc tcttcggcgg tcggtcactt gctcgagctc
     1921 gcggtgccgc aggactacga ggtaaagaag ggcaagtggt ccttcaagca ccttcccgtg
     1981 atccctccgc atttcgacct gtcgccgatt gagaggagtc agcagcgcct caacgtgctg
     2041 ctacgcctga tcaaacgcaa ggacgttacc ggagtcatca acgcctgcga cgcggggcgc
     2101 gaaggcgagc tgatcttccg ttaccttctg cagcatgccc gggcgaaaaa gccgatcgag
     2161 cgcctctggc tgcaatccat gacacagcag tcgatccggg atggcttcgc aaaactacgg
     2221 cccgacaagg atctgctgcc cctcgccgac gccgcgaaga gccgctccga ggccgactgg
     2281 ctggtcggca tcaacggcac gcgggccatg actgccttca attccaagga aggcggcttc
     2341 tatctgacga cggtaggccg cgtgcagaca ccgacgctcg cgatcctcgt cgagcgcgag
     2401 gacaagatcc gcgcctttac gccgcgcaac tactgggaag tgcatgcccg cttcggcgca
     2461 aagaacgggg aatacgccgg acgctggctc aattccggct tcaagaagga tgaggatgcc
     2521 gagcgcaagc cggaacggct gtggaatgct gcggccgcgg cgaagatcgt cgctgcctgc
     2581 cgaggcaaga agggcatcgc gaccgaggaa tccaagccga ccacgcagct ctcgccgctg
     2641 ctgttcgacc tcacgagcct gcagcgcgaa gcgaacgggc gcttcggctt tccggcgcgc
     2701 atgacgcttt cgcttgcaca agcgctgtac gagaagcaca aggtcctcac ttatccgcgc
     2761 accgactcgc gggccctgcc cgaggactac ctgccgacgg tgaaggacgc gctgaaggcg
     2821 cttggcgaaa ccaccgcgtt ctccgcgttc tcgcgccaga tcctcaagc
//
LOCUS       DASPXW010000075        11720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_275900, whole genome shotgun sequence.
ACCESSION   DASPXW010000075 DASPXW010000000
VERSION     DASPXW010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11720
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275900"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /gene="ftsW"
                     /locus_tag="VEL04_01740"
     CDS             <1..950
                     /gene="ftsW"
                     /locus_tag="VEL04_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HYT47119.1"
                     /translation="TVFLFPVRLWQKLAPWLFVGGVVLLAAVLLPGLGREVNGARRWL
                     DVPVVSVQPSELMKLAAVLYAADYTVRKHAVLKSFKRGLLPMLGVMLLVSWLLLREPD
                     FGALVVIAVTAFGILFLGGMNGRHFAALVGMLAAGSALLVVSSPYRMQRIFGFMDPWS
                     DPFGRGYQLSHALIAFGRGEWLGVGLGASVEKLHYLPEAHTDFLLAVIAEELGFAGVA
                     LVIALFVWIVARAFAIGRRASLHERHFAALAAQGIGIWIGFQALVNMGVNMGLLPTKG
                     LTLPLMSYGGSGLLANFVALAILLRIDWENRQLTRGLHA"
     gene            947..2002
                     /gene="murG"
                     /locus_tag="VEL04_01745"
     CDS             947..2002
                     /gene="murG"
                     /locus_tag="VEL04_01745"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HYT47120.1"
                     /translation="MSHTILIMAGGTGGHIFPGLAVAEQLRAAGWEVVWMGARGGMEE
                     RLLPRYGYRAAWIRARAARGKGIVQKLLLPANLLFSFWESARHIRRLKPSVVLGLGGY
                     VAFPGGMMASLLDRPLALHEQNAIPGLANRVLARVCDRVMVAFPDALPGAEWTGNPVR
                     AEIAAIAPPEERLQGRDGPLRLLVVGGSLGAQALNNALPEALALLDAKFLVVHQSGEK
                     HLEALQANYRAAGVEGELVAFIEDMARRYAEADLVICRAGAVTIAELSAGGMASILVP
                     FPHAVDDHQTANAHFLADRGAAILLPQKELSAEKLAELLRMLDRPKLLEMAKKARALG
                     KPEAAHVVARRCMELAR"
     gene            1999..3375
                     /gene="murC"
                     /locus_tag="VEL04_01750"
     CDS             1999..3375
                     /gene="murC"
                     /locus_tag="VEL04_01750"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HYT47121.1"
                     /translation="MKHKVRRVHFVGIGGSGMSGIAEVLVNLGYQVSGSDLAENAATK
                     RLAGLGVKVKVKHHPENVNGADAVVVSTAVRPDNPEVIAARERRIPLVPRALMLAELM
                     RLKQGIAIAGTHGKTTTTSLVASILAEDGLDPTFVIGGRLIAAGSNARLGAGDFIVVE
                     ADESDASFLHLQPVIAVVTNIDADHMDTYQHDFGRLRQAFVQFLQNLPFYGAAILCAD
                     DPNVRAILPLVSKPVLAYGTASDASVRAEAIEHLAGRMRFRAHRAGAAALEVTLNLPG
                     RHNVLNALAAIAVASELGIAEKAILKALSEFRGVGRRFQVYGEVPAQSGGVYTLIDDY
                     GHHPAEMAATLEAARGAFPGRRVVLAFQPHRYSRTRDLFEDFVRVLSKADALLLAEVY
                     AAGEAPIVAADSRSLARALRVAGKVEPVFVEDIGALAEAIRRVALDGDVVVTMGAGSI
                     GNVPAQLT"
     gene            3378..4283
                     /gene="murB"
                     /locus_tag="VEL04_01755"
     CDS             3378..4283
                     /gene="murB"
                     /locus_tag="VEL04_01755"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HYT47122.1"
                     /translation="MSETLQFQGLRGQLRKDEPMARHVSWRAGGPADRLYVPADLDDL
                     AAFLRQLPAGEALLFAGLGSNLLVRDGGWRGTVVLTHAASRRPLMDAGLVYAEAGVAS
                     PKVARFAALHQLEGAEFLAGVPGTVGGALAMNAGCYGGETWDIVERVTTVDRSGRIYQ
                     RTRQDFDISYRQCAPKTAREEWFAAAWFQLKPGDGDTSRGVMKELLARRIATQPLSLP
                     NAGSVFRNPPRDHAARLIEASGLKSFARGGARVSEKHANFIVNPNGAASAADIEWLIQ
                     HIQRMVYQMKGVVLATEVRIVGEPA"
     gene            4280..5200
                     /locus_tag="VEL04_01760"
     CDS             4280..5200
                     /locus_tag="VEL04_01760"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HYT47123.1"
                     /translation="MSPGAKFGKVAVLLGGKSAEREVSLKSGGMILKALQSRSVDAHA
                     FDPAERGLEALIDERFEQVFIALHGRFGEDGTVQGILEWLGIPYTGSGVLASALAMDK
                     LRTKRLWLAEGLPTPPYEVLTKETNLRAVARKLGLPLMVKPANEGSSIGMSKVLAAAG
                     LEEAYALAVNYDPVVIAEKFVDGAELTVGILGEQVLPIIKLETPRDFYDYDAKYIADD
                     TRYLIPCGLSEKKEKELQTLCMKAFRTLGCEGWGRVDLMLNRRGRPYLLEVNTVPGMT
                     DHSLVPMAARAAGLSYPDLCLRILELAHVG"
     gene            5190..5900
                     /locus_tag="VEL04_01765"
     CDS             5190..5900
                     /locus_tag="VEL04_01765"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HYT47124.1"
                     /translation="MWDNPRQLNALAGFLVGLAGLALALAAMQLVLHSGLWQLREVSV
                     RGELKHTTRVQIEAVLQGRVTGNFFDIALPEVRAGLEQLPWVRQASVRRVWPDRLEVS
                     VEEQVPLARWGDGALVNTFGERFAGRSDAPLPQFIAPAGSEGEVARRYARFAQTLAPL
                     GAEIEQVVLTPRFAWQLRLSNGLHLVLGRDADAAQARLERFAEVFAATLGKTGRRYDY
                     VDLRYPNGFALRAPGLRG"
     gene            5908..7137
                     /gene="ftsA"
                     /locus_tag="VEL04_01770"
     CDS             5908..7137
                     /gene="ftsA"
                     /locus_tag="VEL04_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014898179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HYT47125.1"
                     /translation="MAKDNKNLIVALDIGTAKVTCLVAELRAGGALEILGMGGHESKG
                     LKKGVVVNIEATVSAIQRALEEAELMADCKIASAFVGIAGSHIRSFNSTGMVAIKERE
                     VSALDVERAIDTARAVNIPTDQQILHVLRQEFIIDGQEDVREPIGMSGVRLEVKVHIV
                     TGAVSAAQNILKCVRRCGIEVNDLILQPLASSRAVLLEDEKDLGVCLLDVGGGTTDIA
                     IFTHGAIRHTAVVPIAGDQITNDIAMALRTPTGDAENIKTRHGVALRQLADPNQMIEV
                     PGIGERAPRSMSRQTLAEVIEPRVEELYSLVQQVLRESGFEELLSSGVVLTGGSSVMQ
                     GMVELGEEIFHMPVRVGAPRYGGGLAEVVRTPRYATAVGLLMEGVSQVQHGKLSRQSG
                     SLRAVLARMREWFQRNF"
     gene            7183..8337
                     /gene="ftsZ"
                     /locus_tag="VEL04_01775"
     CDS             7183..8337
                     /gene="ftsZ"
                     /locus_tag="VEL04_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HYT47126.1"
                     /translation="MFEIVDSQATGPIIKVIGVGGAGGNAINHMIDQDVQGVEFIAVN
                     TDAQVLSRNKARNQIQLGSSGLGAGAKPEEARAVALAERDRIEEAIAGAHMVFITAGM
                     GGGTGTGAGPVIAEVARSLGILTVAVVTKPFTFEGSKRMKIAEQGLEELAPHVDSLIV
                     ILNDKLEEVLGEDVTQEDAFRAADDVLNNAVAGIAEIINLPGLVNVDFQDVRTVMSEQ
                     GMAMMGSSAASGIDRARIAAEQAIACPLLEGVNLAGARGVLVNITASRASLKLRETKE
                     VMNIIRSFAAEDATIIYGGVYDDALAENLRVTVVATGLGMAAAARQPKLVVSQKTGTD
                     NLPGPGPVDYTQLDAVPAVIRRNRASTVEALQQSGVDKYDIPAFLRKQAD"
     gene            8417..9331
                     /gene="lpxC"
                     /locus_tag="VEL04_01780"
     CDS             8417..9331
                     /gene="lpxC"
                     /locus_tag="VEL04_01780"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HYT47127.1"
                     /translation="MLRQRTLKSLISASGVGLHTGQKVRMTLRPAPADTGIVFRRIDL
                     ASPVDLPARAEHVGDTRMASCLVRDGVRISTVEHLMSALGGLGVDNAYVDLDAPELPI
                     MDGSASPFVLLIQQAGIEEQAALKRFLRVIRRVEVRNGDKWARLEPYDGFKLSFSIDF
                     RHPVIERSTQSVSVDFAETSYLKEIARARTFGFMHEVEDLRESGFALGGRLDNAVVLD
                     EYRVLNAEGLRFADEFIRHKLLDAMGDLYLLGRPLLGAFSAHKSGHALNNRLLRALLA
                     DQTTFETVTFERAEEAPAGVIRLLAQYG"
     gene            complement(9345..9785)
                     /locus_tag="VEL04_01785"
     CDS             complement(9345..9785)
                     /locus_tag="VEL04_01785"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HYT47128.1"
                     /translation="MAEGELQPLMAKARDLRALAGLLDAFLPPDLVGQATVANFREGE
                     LVLLGANAAAAAKLRLLAPSLSGYLSKQHFQVNSVSIRVQPNASRALPVATQKNVRLS
                     THTINTLRALHDRMRASPAREALGRLLERQGGTAALPQTKKPRG"
     gene            9845..10777
                     /locus_tag="VEL04_01790"
     CDS             9845..10777
                     /locus_tag="VEL04_01790"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HYT47129.1"
                     /translation="MQIILISDRLAKARSVSLSLRHLIGTALFALLLVCGATAGLYWL
                     TLRYASEVRAPALQRLVLAAQQAEAERGRTFVQQNLNAMAVKLGEMQAQLTRLDALGE
                     RLSALSGIREFRFSEVPGLGGAAPTLLPPQNLSLTEFNDKLSLLSRQVETRNDMLGVL
                     EAQLFEQAVKKKLTPTMMPVSAPFNASGFGRRIDPFSGQWAMHEGIDFLADIGSPVVA
                     AAGGIVQFSGFHPQYGYMIDIDHGNDLVTRYAHLSKLFVKEGDVVQRARRIALSGNTG
                     RSTGPHVHFEVRFRGTAQNPAKFVAATSTVARAK"
     gene            10913..>11720
                     /locus_tag="VEL04_01795"
     CDS             10913..>11720
                     /locus_tag="VEL04_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HYT47130.1"
                     /translation="MPTRLFGSRNERLLKQYSRAVAQTNALEPEVAALSDAALAGKTD
                     ELKKRYADGATLDDVLPEAFAVVREAATRTLRMRHFDVQLLGGMVLHHGKIAEMKTGE
                     GKTLVATLPAYLNALTGKSVHIVTVNDYLAKRDAEWMGKIYRFLGLSVGVNLPHMDPE
                     DKRKAYAADITYGTNNEFGFDYLRDNMTMHVGERFQRGLHYAIVDEVDSILIDEARTP
                     LIISGMVADSAKWYVTFARLVPRLKPEVDYEVDEQKHTVAVLETGVEKVER"
BASE COUNT         1957 a         3757 c         4018 g         1988 t
ORIGIN      
        1 tcaccgtttt cctgttcccg gtgcgcctgt ggcagaagct cgcgccgtgg ctgttcgtcg
       61 gcggggtggt gctgctcgcc gcggtgctcc tgcctggcct cgggcgcgaa gtgaacggcg
      121 cgcgccgctg gctcgatgta cccgtggtga gcgtccagcc ctcggagctc atgaagctcg
      181 ccgcggtgct ctatgcggcc gactacacgg tgcgcaagca cgccgtcctg aagagtttca
      241 agaggggtct tctgccgatg ctcggcgtga tgctgctcgt ctcgtggctc ctgctgcgcg
      301 agcccgactt cggcgcgctg gtggtgatcg cggtcacggc cttcggcatc ctgttccttg
      361 gcggaatgaa cggcaggcac ttcgcggcgc tggtcggcat gctggcggcg ggatccgcgc
      421 tgttggtggt gtcctcgccc taccggatgc agcgcatttt cgggttcatg gatccctggt
      481 cagacccgtt cgggcgcggc taccagcttt cgcatgcgct gatcgccttc ggccgcggcg
      541 aatggctcgg cgtcgggctc ggagcgagcg tcgagaagct gcactacctg cccgaagcgc
      601 acaccgattt cctgctcgcc gtgatcgccg aggagctcgg cttcgcgggc gtcgcgctgg
      661 tgatcgcgct gttcgtctgg atcgtggcgc gcgccttcgc catcgggcgc cgcgcgagcc
      721 tgcacgagcg gcatttcgcc gcgctcgccg cgcagggcat cggcatctgg atcggattcc
      781 aggcgctggt caacatgggc gtaaatatgg gtctgctgcc gaccaagggc ctgacgcttc
      841 cactcatgag ctacggcggc tcggggctgc tggcgaactt cgttgcgctc gccatcctgc
      901 tgcgcatcga ctgggagaac cgccagttga cgcgaggact gcacgcatga gccacaccat
      961 cctgatcatg gcgggcggca ccggcggtca cattttcccc ggtctggcgg ttgccgagca
     1021 gttgcgcgcc gcaggctggg aggtggtgtg gatgggggcg cgcggcggca tggaggagcg
     1081 gctcctgccg cgttacggct accgcgcggc gtggatccgc gctcgcgccg cgcgcggcaa
     1141 ggggatcgtg cagaagctgc tgcttccggc caacctgctg ttctcgttct gggaaagcgc
     1201 gcgccacatc cggcgcctca agcccagtgt agtgctcggc ctgggcggct atgtcgcgtt
     1261 cccgggcggc atgatggcct cgctcctgga ccggccgctg gcgctgcatg agcagaacgc
     1321 cattcccggg cttgccaacc gcgtattggc gcgagtctgc gaccgggtga tggtcgcgtt
     1381 tccggatgcg cttcccggcg ccgagtggac cggcaacccc gtgcgcgcag aaatcgcggc
     1441 gattgcgccg cccgaggagc gcctgcaggg acgcgacggc ccgcttcgcc tgctcgtggt
     1501 gggcggcagc cttggcgcgc aggctttgaa caatgcgttg cccgaggcac ttgccttgct
     1561 cgatgcgaaa ttccttgtgg ttcaccagtc cggcgagaag catctcgaag cgctgcaagc
     1621 gaattaccgc gcggccggcg tcgagggcga actcgtcgcg ttcatcgagg acatggcgcg
     1681 ccgctatgcc gaggccgacc tcgtcatctg ccgcgccggc gccgtgacca tcgccgagct
     1741 ctcggcgggc ggcatggcca gcatcctggt gccgttcccc cacgccgtcg acgaccacca
     1801 gaccgcgaac gcccatttcc tggcggatcg cggcgccgcg attctcctgc cacagaaaga
     1861 attgagtgcg gagaagctcg cagagctcct gcgcatgctc gatcggccga agctcctgga
     1921 aatggcaaaa aaggcgcgcg ctctcggcaa gccggaggcg gcccacgtgg tcgcgcggcg
     1981 ctgcatggag ctggcgcggt gaagcacaag gtgcgtcgcg tccatttcgt cggcatcgga
     2041 gggtctggca tgagcggcat cgccgaggtg ctcgtcaacc tcggctacca ggtgagcggt
     2101 tcggacctcg ccgagaacgc ggccaccaag cggctcgccg gactgggggt aaaagtaaaa
     2161 gtcaaacacc atccggaaaa tgtcaacggc gccgacgcgg tggtggtgtc gaccgcggtg
     2221 cggcccgaca accctgaagt gatcgcggcg cgcgagcggc gcattccgct tgtgccgcgc
     2281 gccctgatgc tcgccgagct gatgcggctg aagcagggca tcgcgatcgc cggcacacac
     2341 ggcaagacca ccaccaccag cctggtcgcc agcatcctcg ccgaggacgg gctcgacccc
     2401 acgttcgtga tcggcggccg cctcatcgcc gcgggctcga acgcgcggct cggcgccggc
     2461 gatttcatcg tcgtcgaggc ggacgaatcc gacgcctcgt ttctgcacct gcagccggtg
     2521 atcgccgtgg tcaccaacat cgacgccgac cacatggata cctatcagca cgacttcggc
     2581 cgcctcagac aggcgttcgt gcagttcctg cagaaccttc ccttctacgg cgctgcgatc
     2641 ttgtgcgcag acgacccgaa cgtgcgcgcg atcctgccgc tcgtttcgaa gccggtgctt
     2701 gcctacggca cggcgagcga tgcgtcagta cgcgccgagg cgatcgagca cctggccggc
     2761 cggatgcgct ttcgtgccca ccgcgctggc gcggctgcgc tcgaggtgac gctcaacctt
     2821 cccgggcgcc acaacgtgct gaatgcgctt gccgcgatcg cggttgccag cgaactgggc
     2881 atcgccgaaa aggcgatcct caaggcgctg agcgaattcc gcggcgtcgg acgtcgcttc
     2941 caggtctatg gcgaggtgcc ggcgcagtcc ggcggggtct acaccctcat cgatgattac
     3001 ggccatcacc ccgccgagat ggcggcgacg ctcgaggcgg cgcgcggtgc ctttccgggc
     3061 cggcgcgtgg tgctcgcatt ccagccgcat cgctactcgc gcacgcgcga cctgttcgag
     3121 gatttcgtga gagtgctgtc gaaggcggac gcgctgctgc tcgccgaggt ctacgcggcc
     3181 ggcgaagcgc cgatcgtagc ggcggacagc cgctcgctgg cgcgagcatt gcgcgttgcc
     3241 ggcaaggtcg agccggtgtt cgtcgaagac atcggcgcgc tcgctgaagc aattcggcgc
     3301 gtcgcgctgg atggcgacgt cgtcgtcacg atgggcgcgg gctccatcgg caacgtgccg
     3361 gcacaactga catgaacatg agcgaaacgc tccaattcca agggctgcgc gggcaactaa
     3421 ggaaggacga gccgatggcc aggcacgtga gctggcgcgc cggcggaccg gccgaccggc
     3481 tctacgttcc cgcggacctg gacgatctcg cggccttcct gcggcagctt cccgctggcg
     3541 aggcgctgct tttcgccggg ctcggctcga acctgctggt gcgcgacggc ggctggcgcg
     3601 gcaccgtggt gctgacgcat gccgccagcc ggcgcccgct catggacgca ggtttggtgt
     3661 atgcggaagc cggcgtcgcg agcccgaagg tagcgcgctt cgccgcgctg catcagctcg
     3721 agggcgccga atttcttgct ggcgttccgg gcacggtcgg cggggcgctt gccatgaacg
     3781 ccggatgcta tggcggcgag acctgggaca tcgtcgagcg cgtcaccacc gtcgatcgca
     3841 gcggcaggat ctaccagcga acacggcagg acttcgatat cagctaccgg caatgcgcgc
     3901 caaagacggc gcgcgaagaa tggtttgccg cggcctggtt ccagctcaag cccggggacg
     3961 gcgacacgtc gcgtggcgtg atgaaggagc tgctggcgcg gcgcatcgcc acgcagccgc
     4021 tgtcgctgcc gaacgcggga agcgtcttcc gcaacccgcc gcgcgatcac gccgcacggc
     4081 tgatcgaggc cagtgggctc aagagctttg cgcgcggcgg cgcgcgcgtt tccgagaagc
     4141 acgcaaattt catcgtcaat ccgaacggcg cggcgagcgc tgcggacatc gagtggctca
     4201 tccagcacat ccagcgcatg gtgtaccaga tgaaaggcgt ggtgcttgcc accgaagtgc
     4261 gcattgtcgg ggagccggca tgagtccggg ggcgaagttt ggaaaagtgg cggtgctact
     4321 gggtggtaaa tcggccgaac gggaggtctc gctgaagagc ggcggcatga tactcaaggc
     4381 gctgcagagc cgcagcgtgg acgcgcatgc cttcgatccc gccgagcgcg gcctggaagc
     4441 gctgatcgac gagcgcttcg agcaggtgtt catcgcgctg cacggccgct tcggcgaaga
     4501 cggcacggtg cagggcatcc ttgagtggct gggcatccct tacaccggca gcggcgtgct
     4561 cgcctcggcg ctggccatgg acaagctgcg taccaagagg ctgtggctgg ccgaggggct
     4621 gccgacgcct ccctacgaag tgttgaccaa ggaaaccaac ctccgcgccg tggcgaggaa
     4681 gctaggactg cccctgatgg tgaagccggc gaacgaaggc tcctcgatag gcatgtccaa
     4741 ggtgctcgct gcggcgggac tggaagaggc atatgcgctc gcggtgaact acgacccggt
     4801 ggtgatcgcc gagaagtttg tcgacggcgc cgaactgacg gtggggatcc tcggcgagca
     4861 ggtgttgccg atcatcaagc tcgagacgcc gcgcgatttc tacgactacg acgcgaaata
     4921 catcgccgac gatacgcgct acctcatccc ctgcggtctt tcggaaaaga aggaaaagga
     4981 gctgcagacg ctttgcatga aggcattccg caccctcggc tgcgagggct ggggaagggt
     5041 tgacctgatg ctcaaccggc ggggacgccc gtacctgctg gaagtgaaca ccgtgcccgg
     5101 catgaccgac cattcgctgg tgcccatggc ggcgcgcgcg gcaggcctgt cctatccgga
     5161 cttgtgcttg cgcatcctgg agcttgcaca tgtgggataa ccctcgccag ttgaacgccc
     5221 tggcgggttt cctcgtcggc ctcgccgggc tcgcgctcgc actcgcagcg atgcagcttg
     5281 tactgcactc cggcctgtgg cagctgcgcg aagtgtcagt gcggggcgag ctcaagcaca
     5341 ccacgcgcgt gcaaatcgag gccgtgctgc agggtcgcgt cacgggcaat ttcttcgata
     5401 tcgcgcttcc cgaggtgcgc gcggggctcg agcagctgcc ctgggtgcgg caggcgagcg
     5461 tacgccgcgt ctggcccgac cggctcgagg tgtcggtcga ggagcaggtc ccgctcgcgc
     5521 gctggggcga tggcgccctc gtcaacacgt tcggtgaacg cttcgccggc cgcagcgatg
     5581 cgccgctgcc gcagttcatc gcgcccgccg gcagcgaagg cgaggtggcg aggcgctatg
     5641 cgcgttttgc gcagaccctg gcgccgctcg gcgccgagat cgagcaggtg gtgctgacgc
     5701 cgcgctttgc ctggcagctg cggctatcaa acggcctgca tctggtcctc ggacgcgatg
     5761 ccgacgccgc gcaggcgcgc ctcgagcgct tcgcggaggt attcgcagcg acgttgggca
     5821 agaccgggcg gcgctacgac tacgtggacc tgcgctatcc gaacggcttc gccttgcgcg
     5881 cgccggggct caggggctag ggcgcggatg gccaaggaca acaagaacct catcgtggct
     5941 ctcgacatcg gaaccgccaa ggtaacctgc ctcgtcgcgg agctgcgggc cggcggcgcg
     6001 ctcgagatcc tcggcatggg cggccacgaa tccaaggggc taaagaaggg cgtggtggtc
     6061 aatatcgagg ccacggtcag cgccatccag cgcgcactgg aggaggccga gctgatggcc
     6121 gattgcaaga tcgcctctgc tttcgtcggc atcgctggca gccacatccg cagcttcaac
     6181 tctacgggca tggtggcgat caaggagcgg gaagtcagcg cgctcgatgt cgagcgcgcc
     6241 atcgataccg ctcgcgccgt gaatatcccc accgaccagc agatcctgca cgtcctgcgg
     6301 caagaattca tcatcgacgg ccaggaagac gtgcgcgagc cgatcggcat gtcgggcgta
     6361 cggctcgagg tcaaggtgca catcgtcacc ggcgcggtgt cggcggcgca gaacatcttg
     6421 aagtgcgtgc gccgctgcgg catcgaggtg aacgacctca tcctgcagcc gctcgcgtcc
     6481 tcgcgcgcgg tgctgctgga ggacgagaag gatctcggcg tctgcctgct ggacgtcggc
     6541 gggggcacca cagacatcgc gatcttcaca catggcgcaa tccgccacac cgcggtggtg
     6601 cccatcgccg gggaccagat caccaacgat atcgccatgg cgctgcgcac ccccacgggg
     6661 gacgccgaga acatcaagac ccgtcacggc gtcgcgctgc gtcagctcgc cgacccgaac
     6721 cagatgatcg aggtgccggg catcggcgag cgggcaccgc gctcgatgtc gcgccagacg
     6781 ctcgccgaag tgatcgagcc gcgggtcgag gagctctact ccctcgtgca gcaggtgctg
     6841 cgcgagtcgg gcttcgagga gctgctgtcg agcggagtgg tgctgaccgg cggctcttcg
     6901 gtgatgcagg gcatggtcga gctcggcgag gagatcttcc acatgccggt gcgcgtaggc
     6961 gcgccgcgct acggtggagg gctcgccgag gtcgttcgca cgccgcgcta tgcgaccgcg
     7021 gtggggcttt tgatggaagg cgtgtcgcag gtgcagcacg gaaagctgtc gcgccagagc
     7081 ggctcgctgc gggccgttct ggcgcgcatg cgagagtggt tccaacgcaa tttttaaggg
     7141 gcaagcgcaa cttctgatgt caaccggcaa ggagataaca acatgttcga gatcgtagac
     7201 agtcaggcaa cggggccgat catcaaagtg atcggggttg gcggcgcagg cggtaacgcc
     7261 atcaaccaca tgatcgacca ggacgtccag ggcgtggagt tcatcgccgt gaacaccgac
     7321 gcgcaggtgc tgtcgcgcaa caaggcgcgc aaccagatcc agctcggctc gagcggtctg
     7381 ggcgcaggcg ccaagcccga ggaggcgcgc gcggtagcgc tcgccgagcg cgatcgcatc
     7441 gaggaagcga tcgccggcgc acacatggtg ttcatcaccg cggggatggg aggtggtacg
     7501 ggcaccgggg caggcccggt gatcgccgaa gtcgctcgtt cgctcggcat cctgaccgtg
     7561 gcggtggtca ccaaaccgtt caccttcgaa ggctccaagc ggatgaagat cgcggagcag
     7621 ggtctcgaag agctcgcgcc acacgtcgac tcgctgatcg tgatcctcaa cgacaagctc
     7681 gaggaggtac tcggcgagga cgtgacgcag gaagacgcgt tcagggccgc cgatgacgtg
     7741 ctgaacaacg cggtggcggg aatcgccgag atcatcaacc tgccgggcct ggtcaacgtc
     7801 gacttccagg acgtgcgcac ggtaatgtcc gagcagggca tggcgatgat gggttcgtcg
     7861 gcggcgagcg gcatcgaccg cgcgcgcatc gcggccgagc aggccatcgc ctgtccgctg
     7921 ctcgagggcg tcaatctcgc aggcgcacgc ggcgtgctcg tcaacatcac ggcgtcgcgc
     7981 gcgagcctga agctgcgcga gacgaaggag gtgatgaaca tcatccgcag ttttgccgcg
     8041 gaagacgcca ccatcatcta cggcggcgtg tacgacgacg cgctcgcgga aaacctgcgc
     8101 gtgacggtcg ttgccaccgg gctcggaatg gcggccgcgg cgcggcagcc gaagctggtg
     8161 gtatcgcaga agaccgggac cgacaacctt cccggccccg ggccggtcga ctatacgcag
     8221 ctcgatgcgg tgcccgcggt gatccgccgc aaccgggctt ccaccgtcga ggcgttgcag
     8281 caaagcggcg tcgacaagta cgacattccg gcctttttgc gcaagcaggc cgactagccg
     8341 ggcgcggcag cggggcaccc caccttgccg gggggtgccc cgtatgcggt gtctgctaga
     8401 atccgcgcgc ccgaccatgc ttcggcaacg cactctcaag tccctgatca gcgcttccgg
     8461 cgtcggtctg cataccggcc agaaagtcag gatgacgttg cgcccggcgc cggccgacac
     8521 cggcatcgta ttcaggcgca tcgatctcgc ctcgccggtc gaccttccgg cccgcgccga
     8581 gcatgtcggc gacacccgta tggcctcctg cctggtgcgc gatggcgtca ggatcagcac
     8641 ggtcgaacat ctgatgtcgg cgctcggcgg actcggtgtc gacaacgctt atgtggatct
     8701 cgacgcgccc gagctgccga tcatggacgg cagcgcctcg ccgttcgtgc tgctcatcca
     8761 gcaggcggga atcgaggaac aggccgcgct gaagcggttc ctgcgcgtga tacggcgcgt
     8821 tgaggtgagg aacggcgaca aatgggcgcg gctcgagcct tacgacggct tcaagctttc
     8881 attctcgatc gatttcaggc acccggtgat cgagcgttcg acgcaatccg tctcggtcga
     8941 cttcgccgag acttcgtatc tcaaggaaat tgcgcgcgcc cggaccttcg gcttcatgca
     9001 cgaggtcgag gatctcaggg aaagcggctt cgcgctcggc ggccggctgg acaatgcggt
     9061 ggtgctagac gagtaccggg tcctcaacgc cgaggggctg cgcttcgccg acgagttcat
     9121 ccggcacaag ctgctggatg ccatgggcga tctgtatctt ctcggcaggc ctctgctggg
     9181 tgcgttcagc gcgcacaagt cgggtcacgc cctcaacaat cgcctgttgc gcgcgctgct
     9241 cgccgaccag acaacgttcg aaaccgtgac gttcgagcgc gccgaggaag cccccgccgg
     9301 ggtcattcgc cttcttgcgc agtacggctg attccagccg tacgtcaacc ccgaggtttc
     9361 ttggtttgcg ggagggccgc ggtgccaccc tggcgctcta gcagcctgcc cagcgcctcg
     9421 cgcgctggtg aggcccgcat ccgatcgtgc aaagcgcgca aggtattgat cgtatgtgtg
     9481 gataaacgca cgtttttttg cgtcgcaaca ggcaatgcgc gtgacgcgtt tggttgcacc
     9541 cgtatggaaa ctgaattaac ctgaaagtgc tgttttgaca aataaccgct cagggaaggg
     9601 gcgaggagcc tgagtttggc tgccgctgcg gcgttcgcgc caagcagcac tagttctccc
     9661 tccctgaaat tggccaccgt ggcctgacca acaaggtccg gcggcaagaa ggcgtcaagc
     9721 agcccggcga gcgcgcgcag gtcccgcgct ttcgccatga gtggctgcag ttcgccctcg
     9781 gccaagagaa tgcggtcgag ttttggaagt cgcacgtggg atcgcgtact cgggagggag
     9841 acgtgtgcag attatcctga tctccgaccg cctggccaag gcgaggtccg tcagcctgtc
     9901 gttgcgtcat ctcatcggca cggcgctgtt tgcgctgctg ctggtttgcg gcgcgacagc
     9961 ggggctgtac tggctgacgc tgcgctatgc ctcggaggtt cgcgctccgg cattgcaacg
    10021 gctggtgctc gccgcccagc aggccgaagc cgagcgcggc cgcactttcg tgcagcagaa
    10081 tctgaatgcc atggcggtaa agctcggcga aatgcaggcg cagctcaccc gccttgacgc
    10141 gctcggcgag cggctctcgg cgctctcggg catccgcgaa ttccgctttt ccgaggtgcc
    10201 ggggctgggc ggcgccgcac ccaccctgct gccgccgcag aatctttcgc tcaccgagtt
    10261 caacgacaag ctttcattac tgtcacgcca ggtcgaaacc cgcaatgaca tgctgggtgt
    10321 gctcgaggcg cagctgttcg agcaggcggt gaaaaaaaag ctcacgccga cgatgatgcc
    10381 ggtgagcgcc ccgtttaacg cttcgggatt cggccgccgc atcgacccgt tcagcggaca
    10441 gtgggcgatg cacgagggca tcgacttcct cgcggatatc ggctcgcccg tggtcgctgc
    10501 cgcgggcggc atcgtgcagt tctccggctt tcatccgcaa tacggctaca tgatcgacat
    10561 cgaccacggc aacgacctgg tgacgcgcta tgcgcatctt tccaagctgt tcgtgaaaga
    10621 aggcgatgtc gtgcagcgag cgcggcgcat cgcgctatcc ggcaacaccg gtcgctccac
    10681 cgggccgcac gtgcatttcg aggtgcggtt ccgtggcacg gcgcagaacc cggccaagtt
    10741 cgtcgccgct accagcacgg ttgcgcgcgc gaagtaggcc tggcgcgtcg gggcgttccg
    10801 ccccgcgtga taaactctcg aattttccgg gggttggtac acctagatcc ccgcgttcat
    10861 gagcgccaaa atccttcacg ttctgcgtag gttgttggaa gttctctgga gcatcccgac
    10921 gcggttgttt ggcagccgca acgagcgcct gctcaagcag tactcccgcg ccgtggcgca
    10981 gaccaacgca ctggagcccg aggtagcagc gctttcggac gccgccctcg ccggcaagac
    11041 ggacgagttg aagaagcgtt atgcagacgg cgccacgctc gacgatgtgc tgccggaggc
    11101 atttgccgta gtgcgcgagg cggcgacgcg cactctgcgc atgcgccatt tcgacgtcca
    11161 gctgctcggc ggcatggtgc tgcaccacgg gaagatcgcc gagatgaaga ccggcgaggg
    11221 caagacgctg gtcgccacgc tgccggctta tctgaatgcg cttaccggca agagcgtgca
    11281 tatcgtcacg gtgaacgact acctggcgaa gcgcgacgcc gagtggatgg gcaagatcta
    11341 ccgcttcctc ggcctgtctg tcggcgtcaa cctgccgcac atggatcccg aggacaagcg
    11401 caaggcgtac gcggccgata ttacatacgg taccaacaac gagttcggct tcgactacct
    11461 gcgcgacaac atgacgatgc atgtcgggga gcgcttccag cgcgggctgc actacgccat
    11521 cgtcgacgag gtggactcga tcctcatcga cgaggcgcgg accccgctga tcatcagcgg
    11581 catggtggcg gactcggcca agtggtacgt cacgttcgcc cggctggtcc cccggctgaa
    11641 gcccgaggtg gactacgagg tggacgagca gaagcacacg gtggcggtcc tcgagaccgg
    11701 ggtggagaag gtggagcgag
//
LOCUS       DASPXW010000076         3234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1537038, whole genome shotgun sequence.
ACCESSION   DASPXW010000076 DASPXW010000000
VERSION     DASPXW010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1537038"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VEL04_01800"
     CDS             <1..194
                     /locus_tag="VEL04_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HYT47131.1"
                     /translation="IGYLRTVEHPTEGRIKSLAVPSEWSESAPEYRRHAPRLGEHTRE
                     VLREAGFAEEKIDQIIKSI"
     gene            257..1393
                     /locus_tag="VEL04_01805"
     CDS             257..1393
                     /locus_tag="VEL04_01805"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HYT47132.1"
                     /translation="MNAPHDASSVGAVTAQKARFDSPIKLRGGAELPSFELAYETYGE
                     LNAARSNAVLVCHALNASHHVAGFYEHDPGNVGWWDNLVGPGKPLDPRRFFVVGNNYL
                     GSCFGSTGPASTNPATGKRWGADFPVVTVEDWVEAQARLADHLGIERFAAVIGGSLGA
                     MQALQWALSYPERIRHAIVVAAAARLTPQNIAFNEVARQAIMTDPDFHGGHYYEKGVV
                     PVRGLRLARMIGHITYLSGEAMAEKFGRLLRRGAPGFDFDVDFEIESYLRHQGDKFST
                     YFDANTYLRITKALDYYDPAADFGGDLSAALARAKAAFLLVSFESDWRFPPPRSREIV
                     RALLDNRRIVSYLELDAPGGHDAFLLEDARYHAALRAYFGNIEL"
     gene            1390..2016
                     /gene="metW"
                     /locus_tag="VEL04_01810"
     CDS             1390..2016
                     /gene="metW"
                     /locus_tag="VEL04_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003262992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HYT47133.1"
                     /translation="MNSAERTLLAGRADFAAIAQWVAPAARVLDLGCGDGSLLRSLWQ
                     EREAPGYGVEIDDDSVIACVANDVNVLQVDLESGLSLFADGSFDCVILSETLQTIHRT
                     ELLMREMLRVGREVIVSFPNFGHWSARLQVALGRMPVSEALPYEWYETPNVHHCTISD
                     FEDLCRRLGVRIRERLVLHQGKPVAFLPNLFGSLAVYRCNSSITRGAA"
     gene            2013..2474
                     /locus_tag="VEL04_01815"
     CDS             2013..2474
                     /locus_tag="VEL04_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HYT47134.1"
                     /translation="MTRLFFGLRAIALFEAAKGALVLIAGFGLAALVHRDAQHIAERL
                     VHRLHLDAAKRYPRIFLDLMSNVSDAQLWALAALAAVYAVLRFAEAYGLWFGRRWGEW
                     IAAVSGGIYVPVELYELSRGVSWIKLGALLLNTAVVGYMCYILWRGARRQI"
     gene            2474..2671
                     /locus_tag="VEL04_01820"
     CDS             2474..2671
                     /locus_tag="VEL04_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47135.1"
                     /translation="MRTYLVVSGVIFGAVALMHILRLSLNWQVEVAGWSVPLWFSWLG
                     LLVAGALCVWGLRLAAKGTAP"
     gene            complement(2672..>3234)
                     /locus_tag="VEL04_01825"
     CDS             complement(2672..>3234)
                     /locus_tag="VEL04_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HYT47136.1"
                     /translation="IGDTLANLTLRLLFQDLGFDNDEVASYDVAVGFAALLVGIFVGG
                     ILYVRLGMKRSVLSSLVLMAVSNLSFAALAAAGHTNFGMAAAISFENFASGIGGVTVV
                     AYLSALCNLRFTATQYALLSALASIAGRFLTGTSVGALIDAIGYVNFYLLTALVALPG
                     VILFWFMIRSGLADLSVGSAGRDSVS"
BASE COUNT          545 a         1065 c         1048 g          576 t
ORIGIN      
        1 cgatcggcta cctgcgcacc gtggagcatc cgaccgaagg ccgcatcaag tcgctcgctg
       61 tgccctcgga gtggtccgag tcggcgccgg agtatcgccg tcacgcgccg cgcctgggcg
      121 agcacacgcg cgaggtattg cgcgaagccg ggttcgctga agagaaaatc gatcagatca
      181 taaaatcgat ataatctgca gcgcgccgat ttgatgttca gcttgcgggc gcctaacaaa
      241 tgccgctaaa gagaagatga acgctccaca cgatgcttcc tccgtaggcg cggtcacggc
      301 gcagaaggca cgctttgact ctcccatcaa gcttcgcggg ggagccgagc tgccatcctt
      361 cgagctcgcg tatgaaacct acggcgagct gaacgcggcg cgctcgaacg ccgtgctggt
      421 ctgccacgcc ctgaacgcgt cccaccacgt tgccgggttt tacgagcatg acccgggcaa
      481 cgtcggctgg tgggacaacc tggtcggccc cggcaaaccg ctcgacccgc ggcgcttctt
      541 cgtcgtcggc aacaattacc tgggcagctg cttcggctcg accgggccgg cgtcgaccaa
      601 ccccgcgacc ggcaagcgct ggggagccga tttccccgtg gtcacggtgg aagactgggt
      661 cgaggcgcag gcgcggctcg ccgaccacct cggcatcgag cgtttcgccg cggtgatcgg
      721 cggctcgctc ggagcgatgc aggcgctgca atgggcgctc tcctaccccg agcgcatccg
      781 ccacgcaatc gtggtcgcgg ccgcggcccg cctcacgcct cagaacatcg ccttcaacga
      841 ggtcgcgcgc caggcgatca tgaccgaccc tgatttccac ggcggccatt actacgagaa
      901 gggcgtggtg ccggtgcggg gcttgcgact cgcgcgcatg atcgggcata tcacttatct
      961 ttccggcgag gcgatggccg agaaattcgg ccgcctgctg cggcgcggcg cgcccggctt
     1021 cgacttcgac gtcgacttcg agatcgagtc ctacctgcgc caccagggcg acaagttttc
     1081 gacctacttc gacgccaaca cctacctgcg catcaccaag gcgctcgact attacgaccc
     1141 tgccgccgat ttcggcggcg acctgtcggc ggcgctcgcc agggcaaagg cggcgtttct
     1201 gctggtttcg ttcgaatccg attggcgctt cccgccgcca cgctcgcgcg agatcgtgcg
     1261 cgcgctgctc gacaaccggc gcatcgtctc gtatctcgag ctcgacgcgc ccggcgggca
     1321 cgatgccttc cttctcgaag acgcgcgcta ccacgcggcg ctgcgcgcct acttcggcaa
     1381 catcgagctg tgaattctgc cgagcggacg ctgctcgcgg gtcgcgcgga cttcgccgcg
     1441 atcgcgcagt gggtcgcgcc cgcggcgcgc gtgctcgacc tcggctgcgg cgacggctcg
     1501 ctgctgcgct cgctgtggca ggagcgcgag gcgccgggct acggcgtcga gatcgacgac
     1561 gacagcgtga tcgcgtgcgt cgcgaacgac gtcaacgtgc tgcaggtcga tctcgagagc
     1621 gggctgtcgc tgttcgcgga cggctcgttt gactgcgtga tcctttccga gacgctgcag
     1681 accatccatc gcaccgagct cctgatgcgc gagatgctgc gcgtcgggcg cgaggtcatc
     1741 gtcagcttcc ctaacttcgg gcactggagc gcgcgcctgc aggtcgcgct cggccgcatg
     1801 ccggtatccg aggcgctgcc ctacgagtgg tacgagacgc cgaacgtgca ccactgcacc
     1861 atcagcgact tcgaggacct gtgccgccgc ctcggcgtgc gcatccgcga gcgcctggtg
     1921 ctgcaccagg gcaaaccggt ggctttcctg cccaatctct tcggcagcct ggccgtgtat
     1981 cgctgtaaca gctcgatcac gcgcggcgca gcgtgaccag gcttttcttc ggcttacgcg
     2041 ccatagcgct gttcgaagct gccaaaggcg cgctcgtcct gatcgccgga ttcggtctcg
     2101 cagccctggt tcaccgcgac gcccagcaca tcgccgagag gctggtgcac cgcctgcatc
     2161 tcgatgcggc aaagcgctac ccgcgcatat ttctggatct catgagcaac gtctcggacg
     2221 cccagctctg ggcgctcgcc gcgctcgccg cggtgtatgc ggtgctgcgc tttgccgagg
     2281 cgtatggcct gtggtttggc cggcgctggg gagagtggat cgcagcggtg agcggcggca
     2341 tttacgtccc ggtggagctc tacgagcttt cacgcggggt atcgtggatc aagctcggcg
     2401 cactactgct caataccgct gtcgtaggct acatgtgcta catcctctgg cgtggtgctc
     2461 ggagacaaat ctgatgcgaa cttatcttgt ggtttccggc gtcatcttcg gcgccgtcgc
     2521 gctcatgcat atcctcaggc tgtcgctgaa ctggcaagtc gaggtggcgg gctggtcggt
     2581 gccgctctgg ttctcatggc tgggtttact ggtcgctggc gcgctttgcg tctgggggtt
     2641 gcggctcgcc gctaaaggga ccgccccgta atcaggaaac agaatccctg ccagccgagc
     2701 cgactgacag gtcggcaagc ccggagcgga tcatgaacca gaagaggatc acgccgggaa
     2761 gcgcgaccag cgcggtcagc agataaaagt tcacgtagcc aattgcatcg ataagcgccc
     2821 ccacgctcgt tccggtgagg aatcggccgg cgatgctcgc gagcgcagaa agcaaggcat
     2881 attgggttgc agtgaagcgc aagttgcaca atgccgaaag gtaggcgacc accgtcaccc
     2941 cgccgatgcc gctggcgaaa ttctcgaaac tgatcgccgc cgccatgccg aaattggtat
     3001 gaccggccgc ggccagcgcg gcgaagctca ggttcgacac cgccatcagg accaggctgc
     3061 tcagcaccga gcgcttcatg cccagccgca cgtagaggat gccgccgacg aagatgccga
     3121 ccaggagcgc ggcgaagccg acagcgacgt cgtaagaggc gacctcgtcg ttgtcgaagc
     3181 cgaggtcctg gaagagaagc cgcagcgtca ggttggcgag tgtgtcgccg atct
//
LOCUS       DASPXW010000077         5669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1034336, whole genome shotgun sequence.
ACCESSION   DASPXW010000077 DASPXW010000000
VERSION     DASPXW010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5669
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034336"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1217
                     /locus_tag="VEL04_01830"
     CDS             <1..1217
                     /locus_tag="VEL04_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020723075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HYT47137.1"
                     /translation="AEDLLNTIGQLFGPVRGGLAYAVIFVGALLAATTGVVAASVISM
                     GLISLPIMLRYGYDRRLASGVIAASGTLAQIIPPSLVLIVLADQLGRSVGDMYAGAII
                     PGLILTALYASYVLGVSFFYPQAVPALPPEARLLRGWKLVGRVVFVLIPPLALIFLVL
                     GTIFLGWATPTEGGAMGATGALILALINRRLSFALLKQAMNSTAKLTSFVVFILVGAT
                     VFSLTFRGVNGDLWVEHLLTSLPGGQLGFLIVVNILTFLLAFFLDFFELAFIIVPLLA
                     PVADKLGIDLIWFGVLLGVNMQTSFMHPPFGFALFYLRSVAPTREYIDKLSGKPTAPV
                     TTGQIYWGAVPFVVIQVIMVAIVIAFPGMVTGSLEKSTVDPSKVQIEIPQEEGERAPP
                     PADDDLQKQFGK"
     gene            complement(1223..2326)
                     /locus_tag="VEL04_01835"
     CDS             complement(1223..2326)
                     /locus_tag="VEL04_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015421618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HYT47138.1"
                     /translation="MERRSFLKKAAAGVAAGAVAAPAIAQPQPNLQWRLAASWPKSLD
                     TLYGGPDLMARRVAEITDGKFQIRVFAAGEIVPALQVLDAVQAGTVEMGHTAPYYYFG
                     KDPAFAFGTAVCFGGNVRQQNAWWYFGGGSEAMAPLFKDYGCVALLAGNTGAQMGGWW
                     RKEIKTLADMKGVKMRIGGMAGLIMAKIGVVPQQLGAPDIYPSLEKGTIDAAEWVGPY
                     DDEKLGFNKVAKFYYYPGFWEGGPMLMSLVNEKKWNELPKSYQSALHAACGEVNSWMP
                     AKYDAQNPPALRRLIASGTQLRAFPRAVLEAAEKAAYEVYDEMSAKSAHFKRIYGDWR
                     KFRDEQFLWFRVAEGSYDNYTFSSRIGAAPAKK"
     gene            complement(2372..3226)
                     /locus_tag="VEL04_01840"
     CDS             complement(2372..3226)
                     /locus_tag="VEL04_01840"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYT47139.1"
                     /translation="MGIALQSFESVVNKEDARIDLARACLMIAQDAYPGLEVERYLGE
                     IERMAIRLRGRQPDTGGPEERVATLNQFLFDDLGFWGNTEDYYDPRNSYLNEVIDRKT
                     GIPITLAILYMELGRRVGLPLEGVSFPGHFLVRLRLRGGVLVLDPFTGGAMQSEAELR
                     QRLQRVIPEGVADNLPVAELPLDQFLEPASNRQILARVLRNLKGIYRDTDKPERMLDV
                     LNRMLLVAPDASAELRDRGLVYHRLECYRAALKDLSDYAEREPEAPDLDDVRVKLMEL
                     SALCARLN"
     gene            complement(3226..4764)
                     /gene="murJ"
                     /locus_tag="VEL04_01845"
     CDS             complement(3226..4764)
                     /gene="murJ"
                     /locus_tag="VEL04_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003272000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HYT47140.1"
                     /translation="MNLLRTLFTVSSLTLVSRILGYVRDFVIARAFGAGLATDAFFVA
                     FRIPNLLRRLFAEGAFSQAFVPVLAEQKNQTSLEETKKLIDGVATLLGLALVVAAALG
                     IAGATLIVYLSAPGFAADAAKFDLTVKLLQITFPYIAFISLVAFAAGVLNTWNRFSVP
                     AITPALLNVSFIAGALAFADFFDPPILVLAWAVFAGGALQLAFQVPFLSRLGLLPRWR
                     LDFSHPGVRRVLKLMAPAAFGVSISQLSLLINTIFASFLVSGSVSWLYYADRLMEFPA
                     GVLGVALGTILLPSLSKYHADANHEEYGRLLDWGLRLTLLLAVPSAAALAVIALPLIA
                     ALFHYGRFSAGDAWMTREALVAYSIGLVGMILVKILAPGFYARQNIVTPVKIGIVTLA
                     ATQLMNLAFIGPLQHAGLALAIGLGACLNAALLFRMLRKHGIYAPQPGWAIFLLKIAA
                     AAAFMAIVLFTTMGEADWWLAASWQRKLPAVIGLALLGATAYGACLYLFGLRLSQFYR
                     RGAG"
     gene            4801..5064
                     /gene="rpsT"
                     /locus_tag="VEL04_01850"
     CDS             4801..5064
                     /gene="rpsT"
                     /locus_tag="VEL04_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HYT47141.1"
                     /translation="MANIKSARKRARQAIVRRSHNLSLRTAVRSAIKNVKKAVAAGNK
                     DTAAKVLRASQRAIDRVVAKGVMHRNAGDRHKSRLAHAVKAMQ"
     gene            complement(5072..5401)
                     /locus_tag="VEL04_01855"
     CDS             complement(5072..5401)
                     /locus_tag="VEL04_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3579 domain-containing protein"
                     /protein_id="HYT47142.1"
                     /translation="MPDLEIVEFVIQGLTRDGRRFRPSDWAERLCGVMSAFGGDHRMQ
                     YSPFVHPVSANGVRCVVVDARLGQVEPMAYRFLLSFAKDNELQMRDGRVPERTKLAQL
                     QRVGDTA"
     gene            5426..>5669
                     /locus_tag="VEL04_01860"
     CDS             5426..>5669
                     /locus_tag="VEL04_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47143.1"
                     /translation="MAKPSCRILVINDEHLVLREFVKGLNAAARALDNPLGISFTGVA
                     TAKEALAVIESEGNLQAVLVDDRLYTLGSNSKKKLQM"
BASE COUNT          976 a         1909 c         1860 g          924 t
ORIGIN      
        1 tggccgagga tctgctcaat accatcggac agctcttcgg tccggtccgg ggagggctcg
       61 cctacgccgt gattttcgtc ggcgcgctgc tcgccgcgac gaccggcgtg gtcgccgcat
      121 cggtgatctc gatgggcctg atctcgctgc cgatcatgct gcgctacggt tatgaccggc
      181 ggcttgcgag cggcgtgatc gccgcctccg gaaccctcgc ccagatcatc ccgccctcgc
      241 tggtgctgat cgtgctcgcc gaccagctcg ggcgctcggt cggcgacatg tacgccggcg
      301 ccatcattcc gggtctcatc ctgaccgcgc tgtacgcctc ctatgtgctg ggcgtgagct
      361 ttttttatcc gcaagcggta ccggcgctgc cgcccgaagc gcgcctgctg cgcggctgga
      421 agctggtggg ccgcgtggtg ttcgtcctga tcccgccgct cgcgctcatc ttcctggtgc
      481 tcggcaccat ctttctcggc tgggccacgc cgaccgaggg cggggcaatg ggcgcaaccg
      541 gagcgctcat cctcgcgctg atcaatcgcc gcctcagctt cgcgctcctc aagcaggcga
      601 tgaactcgac cgcgaagctc acttcgttcg tagtgttcat cctggtcggc gcgacggtct
      661 tcagcctcac cttccgcggc gtgaacggcg acctgtgggt cgagcacctg ctcaccagcc
      721 tgccgggcgg gcagctcggc tttctcatcg tggtcaacat cctgaccttc ctgctcgcgt
      781 tcttcctcga tttcttcgag ctggcgttca tcatcgtgcc gctgctcgcc ccggtggccg
      841 acaagctcgg catcgacctg atctggttcg gcgtgctgct cggggtcaat atgcagacct
      901 cgttcatgca tccgccgttc ggcttcgcgc tgttctacct gcgcagcgtc gcgccgacga
      961 gagaatacat cgacaagctg tccggcaagc cgaccgcgcc ggtcaccacc ggccagatct
     1021 actggggcgc ggtgcctttc gtggtgatcc aggtgatcat ggtcgcgatc gtgatcgcgt
     1081 tccccggcat ggtgaccggc agcctggaga agagcaccgt cgatccttcg aaggtgcaga
     1141 tcgagatccc gcaggaagaa ggcgagcggg cgccgccgcc cgccgatgac gacctgcaga
     1201 agcagttcgg aaagtaaaag ctctacttct tcgccggcgc ggcgccgatc ctcgagctga
     1261 aggtgtagtt gtcgtagctg ccctcggcga ccctgaacca caggaactgc tcgtcgcgga
     1321 acttcctcca gtcgccgtag atgcgcttga agtgcgcgct cttcgcgctc atctcgtcgt
     1381 acacctcgta ggccgccttt tccgccgcct cgagcaccgc gcgcgggaac gcgcgcagct
     1441 gcgtgccgct cgcgatcagc cggcgcagcg ccggcgggtt ctgcgcgtcg tacttcgccg
     1501 gcatccagga gttgacttcg ccgcaagcag cgtgcagcgc ggactggtag gacttgggca
     1561 gctcgttcca cttcttctcg ttcaccaggc tcatcagcat cgggccgcct tcccagaaac
     1621 ccgggtagta gtagaacttg gcgaccttgt tgaagccgag cttctcgtcg tcgtaggggc
     1681 cgacccattc cgcggcgtcg atggtgccct tctcgagcga ggggtaaatg tccggagcgc
     1741 cgagctgctg cggcaccacg ccgatcttcg ccatgatcag gcccgccatg ccgccgatgc
     1801 gcatcttcac gcccttcatg tcggcgagcg tcttgatctc cttgcgccac cagccgccca
     1861 tctgcgcgcc ggtattgccg gcgagcagcg cgacgcagcc gtaatccttg aagagcggcg
     1921 ccatcgcctc gctgccgccg ccgaaatacc accaggcgtt ctgctggcgc acgttgccgc
     1981 cgaagcacac cgcggtgccg aaggcgaagg cgggatcctt gccgaaatag taataggggg
     2041 cggtgtggcc catctcgacc gtgccggcct gcaccgcgtc cagcacctgc agcgcgggaa
     2101 cgatctcgcc cgccgcgaag acgcggatct ggaatttgcc gtcggtgatc tcggccacgc
     2161 gcctggccat gagatcgggg ccgccgtaga gcgtgtccag gctcttgggc cagctcgccg
     2221 cgaggcgcca ctggaggttc ggctgcggct gcgcgatcgc gggtgctgcg accgcgcccg
     2281 cagcgacgcc ggcagccgcc tttttcagaa aacttctgcg ttccatgtcg ttccccatga
     2341 aggacaaatt tgcggagccg cgattgtatg cctaattaag ccgggcgcag agcgccgaaa
     2401 gctccatcag cttgacgcgc acgtcgtcga ggtcgggcgc ctcgggctcg cgctcggcgt
     2461 agtcggacag gtccttgagc gccgcgcggt agcactccag ccggtggtag accaggccgc
     2521 ggtcgcgcag ctccgcgctc gcgtccggcg ccaccagcag catccggttc agcacgtcga
     2581 gcatgcgctc cggcttgtcg gtgtcgcggt aaatgccctt caggttgcgc agcacgcgcg
     2641 ccaggatctg gcggttcgag gccggctcga ggaactggtc gagcggcagc tcggcgaccg
     2701 gcaggttgtc ggccacccct tcagggatca cgcgctgcag ccgctggcgc agctcggctt
     2761 ccgattgcat cgccccgccg gtgaacgggt cgagcaccag caccccgccg cgcagccgca
     2821 ggcgcaccag gaagtggccc gggaacgaaa ccccttcgag cggcaggccg acgcggcgcc
     2881 cgagctccat gtagaggatc gcgagcgtga tcgggatgcc ggtcttgcgg tcgatgacct
     2941 cgttcaggta gctgttgcgg gggtcgtagt agtcctcggt attgccccag aaacccaggt
     3001 cgtcgaagag gaactggttg agcgtcgcga cgcgctcctc gggtccgccg gtgtccggct
     3061 ggcggccgcg caggcgaatc gccatgcgct cgatctcacc gagatagcgc tcgacctcga
     3121 gcccggggta ggcatcctgg gcgatcatca ggcaggcgcg ggcgagatcg atgcgtgcat
     3181 cttccttgtt gaccaccgat tcaaaagact gcagcgcgat gcccatcatc ctgctccgcg
     3241 gcggtagaac tggctcaggc gcaagccaaa caggtagagg caggcgccgt aagcggtcgc
     3301 gccgagcagc gcgaggccga tcacggccgg gagcttgcgc tgccagctcg cagcgagcca
     3361 ccagtccgcc tcgcccatgg tggtgaacag cacgatggcc atgaatgctg ccgcggcggc
     3421 aatcttgagg agaaagatcg cccaacccgg ctgcggagcg taaatgccgt gctttctcag
     3481 catgcggaac aacagcgccg cgttcaggca ggcgccgagc ccgatcgcga gcgcgagccc
     3541 ggcgtgctgc agcggcccga tgaacgccag gttcatgagc tgggtggcgg cgagggtgac
     3601 gatgccgatc ttcaccggcg tgacgatgtt ctgcctggca tagaaacccg gcgcgagaat
     3661 cttcaccagg atcatgccga cgaggccgat gctgtaagcg acgagcgcct cgcgcgtcat
     3721 ccaggcatcg cccgcgctga accgcccgta gtggaacagc gcggcgatca gcggcagggc
     3781 gatcacggcg agcgccgccg cgctcggcac ggcgagcagc agcgtgagcc gcaggcccca
     3841 gtcaagcagc cgcccgtatt cctcgtgatt ggcgtcggca tggtatttcg acaggctcgg
     3901 cagcaggatg gtgccgagcg cgacgccgag cacccctgcc ggaaactcca tcaggcggtc
     3961 ggcatagtac agccacgaca cgctgcccga gaccaggaac gaagcgaaga tcgtgttgat
     4021 gagcagcgac agctggctga tcgagacgcc gaacgccgcg ggcgccatca gcttcaggac
     4081 ccggcgcacg ccgggatgcg aaaagtccag gcgccagcgc ggcagcaaac ccagcctgga
     4141 cagaaaaggc acctggaacg caagctgcag cgcgccgccg gcgaacaccg cccaggcgag
     4201 cacgagtatg ggcgggtcga agaaatcggc gaatgcgagc gcgccggcga tgaacgacac
     4261 gttcagcagc gcgggcgtga tcgccggcac cgagaagcgg ttccaggtat tcagcacccc
     4321 ggcggcgaat gccaccagcg agatgaacgc gatgtacggg aaggtgatct gcagcagctt
     4381 caccgtgagg tcgaacttgg ccgcatcggc ggcgaacccc ggcgcggaca ggtacacgat
     4441 cagcgtcgcg cccgcgatgc ccagagccgc ggcaaccacc agcgccaggc cgagcagcgt
     4501 cgccacgccg tcgatgagtt tcttggtttc ttccagggaa gtctggttct tttgttcggc
     4561 caggacggga acgaaagcct gggagaacgc accctcggcg aacagccgcc gcagcaggtt
     4621 ggggatgcgg aaggcgacga agaaagcgtc ggtcgcgagc ccggcgccga aggcgcgcgc
     4681 gatcacgaag tcgcgcacgt aaccgaggat cctgctgacc aaggtcaggc tgctcacggt
     4741 gaacagggtg cgcaggaggt tcatgctaaa atccgccccc tttcaggggc ccccgagttc
     4801 atggcaaaca tcaagtccgc gcgcaagcgc gcgcgccagg cgatcgtgcg ccgcagccac
     4861 aatctcagcc tgcgcaccgc ggtgcgcagc gcgatcaaga acgtgaaaaa ggcggtcgcc
     4921 gcgggcaaca aggacaccgc cgcgaaggtg ctgcgtgcgt cgcagcgcgc catcgaccgc
     4981 gtggtcgcca agggcgtgat gcaccgcaac gccggcgacc gccacaagag ccgcctcgcc
     5041 cacgccgtca aggcgatgca gtaagcctgc actaggcagt gtcgccgacg cgttgcagct
     5101 gcgcgagctt ggtgcgctcc ggaacgcggc cgtcgcgcat ctgcagctcg ttgtccttgg
     5161 cgaagctgag caggaaccgg taggccatgg gctccacctg ccccagccgc gcgtcgacca
     5221 ccacgcagcg cacgccgttc gcgctcaccg ggtgcacgaa cggcgagtac tgcatgcggt
     5281 gatcgccgcc gaacgccgac atcaccccgc acaggcgctc cgcccagtcg ctcggccgga
     5341 accgcctgcc gtcacgcgtg agcccctgga tgacgaactc gacgatctcg agatcgggca
     5401 tgcgcgatta tagttataac gactaatggc caagccctcg tgccgcatcc tggtcatcaa
     5461 cgacgagcac ctcgtgctgc gcgagttcgt caagggcctg aacgcggcgg cgcgcgcgct
     5521 cgataacccg ctcggcatca gcttcaccgg cgtcgccacc gcgaaggaag cgctcgcggt
     5581 gatcgagtcc gagggcaacc tgcaggcggt gctggtggat gaccggctct atacgctcgg
     5641 ctccaattca aaaaagaagc tgcaaatgt
//
LOCUS       DASPXW010000078         2333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1791134, whole genome shotgun sequence.
ACCESSION   DASPXW010000078 DASPXW010000000
VERSION     DASPXW010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1791134"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /gene="tadA"
                     /locus_tag="VEL04_01865"
     CDS             complement(<1..334)
                     /gene="tadA"
                     /locus_tag="VEL04_01865"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HYT47144.1"
                     /translation="MTDEEYMRRALELAQRAQEEGEVPIGAVVVMQDEIVGEGWNQPI
                     AAADPTAHAEIQAMRAAAAKLGNYRLSGATLYVTLEPCDMCLGAMFHARIARAVFGAT
                     DPKKPVLKN"
     gene            complement(392..754)
                     /locus_tag="VEL04_01870"
     CDS             complement(392..754)
                     /locus_tag="VEL04_01870"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HYT47145.1"
                     /translation="MLNVDTHILLYAVTGQLTPRERKLLQNDTWSISAIVLWEIAKLA
                     RLGRIGVDLEDADVVRTLARLHIWPLTPEIARASTQLDIRGDPADELIAATSAIHNVP
                     LVTRDLGLLRSKIVPLAR"
     gene            complement(741..959)
                     /locus_tag="VEL04_01875"
     CDS             complement(741..959)
                     /locus_tag="VEL04_01875"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HYT47146.1"
                     /translation="MKYVPAAKFKAQCLSLIDRVDKDGIVITKRGKPVAKLIPLGGDS
                     ASLIGSLKGKVEIKGDLLSTGVAWNAQR"
     gene            complement(1042..>2333)
                     /gene="guaA"
                     /locus_tag="VEL04_01880"
     CDS             complement(1042..>2333)
                     /gene="guaA"
                     /locus_tag="VEL04_01880"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HYT47147.1"
                     /translation="GGRVEAGKVREFGYAEVRARGQTKLLNGIEDSRTPEGHGMLQVW
                     MSHGDKVTQLPPGFKLMASTEACPIAAMADEQRRFYGVQFHPEVTHTRQGTRLLQRFV
                     HDIARCSGDWNMPGYVSEAVAKIREQVGDEEVILGLSGGVDSSVAATLIHRAIGERLT
                     CVFVDHGLLRLDEAAQVMESFAAHKHMKVVHVDAAAEFFAALQGISDPEQKRKIIGKH
                     FVEVFQREAKKIKNAKWLAQGTIYPDVIESAGAKTKKAMTIKSHHNVGGLPESLHLKL
                     LEPLRELFKDEVRELGLALGLRREMVFRHPFPGPGLGVRILGEIKKEYVRLLQRADDI
                     FIQELRNSGWYDKTAQAFAVFLPVRSVGVMGDGRTYENVVALRAVQTTDFMTAEWAPL
                     PHELLARVSSRIINEVRGINRVTYDISSKPPATIEWE"
BASE COUNT          387 a          759 c          730 g          457 t
ORIGIN      
        1 ggttcttgag cactggcttc ttcgggtcgg tggcgccgaa taccgcgcgc gcgatgcgcg
       61 catggaacat cgcgcccagg cacatatcgc agggctcgag cgtgacgtac agggtcgcgc
      121 cgctcagccg gtagttgccg agcttcgccg cggcggcgcg catggcctgg atctcggcat
      181 gcgccgtggg atcggcggcg gcgatcggct ggttccagcc ttcgccgacg atctcgtcct
      241 gcatcaccac gacggcgccg atcgggactt ctccctcttc ctgcgcgcgc tgcgccagct
      301 cgagcgcgcg gcgcatgtac tcctcgtcgg tcactgacgc cggggatgga cctgatggca
      361 cgcgtattcg ttgacggcga cgcgagctgc gtcagcgtgc aagcggcacg attttcgatc
      421 gcagcaagcc aaggtcgcgc gttacgaggg gaacattgtg gatggcgctc gtggcagcaa
      481 tcagctcatc ggctggatcg ccgcggatgt ctagctgcgt gctggcgcgg gcgatctccg
      541 gcgtcaaagg ccagatatgc aagcgcgcga gcgtgcgtac gacgtcggcg tcttccaggt
      601 cgacgccgat gcgcccgagt cgagcgagtt tggcgatctc ccagagaacg atcgcggaaa
      661 tgctccaggt atcgttttgc agcagcttcc tttcgcgcgg ggtcagctgc cccgtcaccg
      721 catagagcag gatatgcgta tcaacgttga gcattccagg cgactccggt cgagagaagg
      781 tcgcctttga tttcgacctt tcccttcagc gagccaatca agctcgccga gtcaccgccg
      841 agcgggatca gcttggcgac gggtttgccc cgcttcgtga tgacaatgcc gtccttgtcg
      901 acccgatcga taagcgatag gcactgcgcc ttgaactttg ccgccggcac gtatttcatg
      961 gacacattat atgactagtc acatcaaatg accacattcg aacggcgccc acccgcggtc
     1021 gtcgcggaaa ggtccgccgg ttcattccca ctcgatggtc gccggcggct tcgacgagat
     1081 gtcgtaggtg acgcggttga tgccgcgcac ctcgttgatg atgcggctcg agaccctcgc
     1141 gagcagctcg tgcggcagcg gcgcccactc ggcagtcatg aagtcggtgg tctgcacggc
     1201 gcgcagggcg accacgttct cgtaggtgcg gccgtcgccc attacgccga ccgagcgcac
     1261 cggaaggaac accgcgaacg cctgagcggt cttgtcatac cagcctgaat tcctgagctc
     1321 ctgaatgaag atatcgtccg cacgctgcaa gagccgcacg tactctttct tgatctcgcc
     1381 gaggatgcgc acgccgagcc ccggtcccgg gaaaggatgg cggaacacca tctcgcgccg
     1441 caggccaagt gcgaggccca gctctctgac ttcgtccttg aacagctcgc gcagcggctc
     1501 gaggagcttg aggtggagcg actcgggcaa ccccccgacg ttgtggtgcg acttgatggt
     1561 catcgccttc ttggtcttcg cgccggccga ctcgatcacg tcggggtaaa tcgtgccttg
     1621 tgccagccat ttggcgttct tgatcttctt cgcttcccgc tggaagacct ccacgaaatg
     1681 cttgccgatg atttttcgct tctgctccgg gtcggaaatg ccctgaagcg cagcgaaaaa
     1741 ttccgccgca gcgtcgacgt ggacaacttt catgtgctta tgggcggcaa agctctccat
     1801 cacctgggct gcctcgtcga gtcgcagcag gccgtggtcg acgaacacgc aggtgagccg
     1861 ctcgccgatt gcgcggtgga tgagcgtggc ggccaccgac gaatcgaccc cgcccgaaag
     1921 cccgaggatg acttcctcgt caccgacttg ctcgcggatc tttgcaacgg cctcggagac
     1981 gtaccccggc atgttccagt cgccgctgca gcgcgcgatg tcgtggacga agcgctgcag
     2041 cagtctggtt ccctgccgcg tatgcgtgac ctccggatgg aactgcacgc cgtagaagcg
     2101 ccgctgctcg tcggccatcg cggcgatcgg acaggcctcg gtcgatgcca tcagtttgaa
     2161 ccccggcggc agctgcgtga ctttgtcgcc gtggctcatc cagacctgga gcatgccgtg
     2221 gccttccggg gttcgcgagt cctcgatgcc gttcagcagc ttcgtctgcc cgcgcgcgcg
     2281 cacttccgca tagccgaact cccgcacttt ccctgcttcg acgcgcccgc cca
//
LOCUS       DASPXW010000079         4940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_530790, whole genome shotgun sequence.
ACCESSION   DASPXW010000079 DASPXW010000000
VERSION     DASPXW010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4940
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530790"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="VEL04_01885"
     CDS             complement(<1..691)
                     /locus_tag="VEL04_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HYT47148.1"
                     /translation="MRYVTDGVQGIRRRRVGKGWAYYTPSGRRIGDSRVRKRLNRLAI
                     PPAWTDVWICPDPGGHIQATARDARGRKQYRYHHSYREARDRSKFSRMLQFSEVLPRV
                     RERIERDLRSADLGRRQMLATVVRLLDRTLIRVGNDEYVRENRSYGLTTLRRRHVRVD
                     GAMLRFEFRGKSGVDHTVTIADPRLARIIQRCRDLPGQELFQYLGASRKRQSVSSEDV
                     NAYLRELTGRDI"
     gene            complement(724..981)
                     /locus_tag="VEL04_01890"
     CDS             complement(724..981)
                     /locus_tag="VEL04_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47149.1"
                     /translation="MSITKELEQLQCEKRKLRREIALAEAEQKDSKELRKRFAVLDKD
                     PRTSKLEKRLGQLEPRLSKSSDAEDLQAISRQQVEAFYRCE"
     gene            complement(1056..2543)
                     /locus_tag="VEL04_01895"
     CDS             complement(1056..2543)
                     /locus_tag="VEL04_01895"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HYT47150.1"
                     /translation="MIKDVLSYGLGTLTALVVAALLVFVFIRDITQKKHSVLRNYPIV
                     GRLRYFFEQLGEYFRQYFFAGDREEMPFNRATRGWVYRLAKNEGGIIGFGSTYDLHQP
                     GALIFVNHPFPVLEEERQPTPTLILGEGYCKQPFEAKSIVNISGMSFGAISAPAVRSL
                     SRGAAVAGCWMDTGEGGLSSYHLEGGCDVIMQIGTAKYGVRDAQGSLSNERLTELAQS
                     VKAFEIKLSQGAKPGKGGVLPGKKVTAEIAQIRGIPEGRDSISPNRHRDISNMNELLE
                     RIAWMRELTGRPVGVKTAVGGWRFMNEMADIVHRRGLEFAPDFLVIDGGEGGSGAAPQ
                     ALADHMGLSIEEALPRVVDSLIESGLKGRVRVVASGKIVTSARAAWALCVGADFINTA
                     RGFMFSLGCIQALRCHQNTCPTGVTTHNKRLQRALVVEEKYLRVANYANNMNREIDMI
                     AHSCGVRHARELKREHVRIVQASGQSVALNMLYPYPEPKAKMKVA"
     gene            complement(2644..3117)
                     /locus_tag="VEL04_01900"
     CDS             complement(2644..3117)
                     /locus_tag="VEL04_01900"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HYT47151.1"
                     /translation="MLQPGQAAPWFELPDADMQTVNLSAFKGKKHVVLYFYPRDGTPG
                     CTMVTTEFSDHEDQFGKHDCVVLGVSRDDCLTHADFRDKNGVTIALLSDPEGEVCRKY
                     GVLQEKETQDGARRECLVRSTFVIDKKGVIRHATYGVNPRGHAAEVFGLIKRLRA"
     gene            3171..4238
                     /locus_tag="VEL04_01905"
     CDS             3171..4238
                     /locus_tag="VEL04_01905"
                     /inference="COORDINATES: protein motif:HMM:NF042820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYT47152.1"
                     /translation="MNSRLLGGLTSREFLRRHWQKRPLLVRRALQGALRLLDERALTA
                     LARRDDVESRIVERRANRWDTAHGPFRKKFHAKKKNWTLLVSGVNLHLAAADALLRRF
                     DFVPQARLDDVMVSFASPGGGVGPHVDSYDVFLVQGPGRRRWRISAPGKSKVQTFIAE
                     PGDLLYLPPGWRHHGVALEPCFTYSIGFRAPRGAELGAAFLDWLHERGLPDAAYRDAG
                     LSPVKNAALIPGEMVVFVTQLLRRIRWSRADAQRFLGEYLTLPKAHVVFRPAPAPGRP
                     APPARGGSPVRLDPKTRLLYRGNRFFMNGDTVTVTGRDARWLRNLADQRRASGAPSGR
                     TRIARLLRSWQELGYLRLERP"
     gene            4239..4724
                     /locus_tag="VEL04_01910"
     CDS             4239..4724
                     /locus_tag="VEL04_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47153.1"
                     /translation="MDEAIYERFDTSAGFQDAVDRLLEQPGRELRVFDPDGAALRLND
                     APRIEGFERFLLASRTRRLYMALHDTEYLTRRCPRMMALLTRFSHAIQINRTHEEIRE
                     LQDAFLVLDAAHYVRRPVAAFFRGAMGLGDETDGLAMRSRFLEIWAASYPAVPATTAG
                     L"
     gene            complement(4737..>4940)
                     /locus_tag="VEL04_01915"
     CDS             complement(4737..>4940)
                     /locus_tag="VEL04_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYT47154.1"
                     /translation="GHLDNRDSERLLGALDRSRLRHVIGAHLSQQNNRPELARAALAR
                     AMGCDESWIGLATQDHGFDWRQA"
BASE COUNT          779 a         1672 c         1621 g          868 t
ORIGIN      
        1 tgatgtcgcg gccggtgagc tcgcgcagat aggcgttgac gtcttcggat gacaccgact
       61 ggcgcttgcg cgaggcgcca aggtactgga acagctcctg cccgggcagg tcgcggcagc
      121 gctggatgat gcgcgcaagg cgcggatcgg cgatcgtcac cgtatgatcg accccgctct
      181 tgccgcggaa ttcgaagcgc aacattgcgc cgtcgacacg cacgtgccgg cggcgcagcg
      241 tggtgaggcc gtaagagcgg ttctcgcgca cgtactcgtc gttgccgacg cggatcagcg
      301 tgcggtcgag cagccgcacc accgtggcga gcatctggcg ccggccgaga tcggccgaac
      361 gcaggtcgcg ctcgatgcgc tcgcgcacgc gcggcagcac ctcgctgaac tgcagcatgc
      421 ggctgaactt cgagcggtcg cgcgcctcgc ggtacgagtg gtggtagcgg tactgcttgc
      481 ggccgcgcgc atcgcgcgcc gtggcctgga tatggccgcc agggtccggg cagatccaca
      541 cgtcggtcca cgccggcgga atcgcgagcc ggttgaggcg cttcctgact ctcgaatccc
      601 caatccgcct gccgctgggc gtgtaatacg cccagccctt gccgacgcgg cggcgccgga
      661 ttccctgcac cccgtcggtg acgtagcgca acccggcgcc gaccgcggcc ttgcggtgct
      721 cggttattcg cagcggtaga acgcctccac ctgctggcgg ctgatggcct gcaggtcctc
      781 ggcatcggag gatttcgaaa ggcgcggctc gagttgcccc agccgctttt ccagcttgga
      841 agttctcgga tccttgtcga gcactgcgaa gcgtttgcgg agctccttcg aatccttttg
      901 ctcggcctcg gcgagtgcga tctcgcgccg cagcttgcgc ttctcgcact gcaactgctc
      961 gagttctttc gtgatgctca tgaattcctc ggcttgcggg gtcaatgccc acgctgggaa
     1021 accaatacct aggaaaaaac tcagggcaat gcgcttcaag cgaccttcat cttcgctttc
     1081 ggttcggggt acggatagag catgttgagg gccaccgact ggccgctcgc ctgcacgatg
     1141 cgcacatgct cgcgcttcaa ttcacgcgca tgacgcacgc cgcaggagtg cgcgatcatg
     1201 tcgatctcgc ggttcatgtt gttggcgtag ttcgctacac gcaggtattt ctcctcgacc
     1261 accagcgcgc gctgcaggcg cttgttatgg gtggtgacgc cggtcgggca ggtgttctgg
     1321 tggcagcgca gcgcctggat gcagccgagc gagaacatga agccgcgtgc agtgttgatg
     1381 aagtcggcgc cgacgcaaag cgcccacgcc gcgcgcgccg aggtgacgat cttgcccgag
     1441 gcgacgacgc gcacgcgccc cttgagcccg ctttcgatca gcgaatcgac cacgcgcggc
     1501 agcgcctcct cgatcgacag ccccatgtgg tcggcgagcg cctgcggcgc cgctccggag
     1561 ccgccctcgc cgccgtcgat aacgaggaag tcgggcgcga actccaagcc gcggcggtgc
     1621 acgatgtcgg ccatctcgtt catgaagcgc cagccgccga ccgcggtctt gacgccgacc
     1681 ggcctgccgg tgagctcgcg catccacgcg attctctcga gcagctcgtt catgttcgag
     1741 atgtcgcggt ggcggttggg gctgatagaa tcgcgcccct cggggatgcc gcggatctgc
     1801 gcaatctcgg cagtgacctt ctttcccggc agcacgccgc ccttgccggg cttggcgcct
     1861 tgcgagagct tgatctcgaa tgccttcacc gattgcgcca gctcggtgag gcgctcgttc
     1921 gagaggctgc cctgcgcatc gcgcacgccg tatttcgccg tgccgatctg catgatgacg
     1981 tcgcagccgc cctcgaggtg gtacgacgag agcccgccct cgccggtatc catccagcac
     2041 ccggccaccg cagcgccgcg cgacagcgag cgcaccgccg gcgcggagat ggcgccgaag
     2101 ctcatgccgc tgatgttgac gatcgacttc gcctcaaagg gctgcttgca gtagccctcg
     2161 ccgagaatca gcgtcggcgt cggctgcctc tcttcctcta gcaccgggaa cgggtggttg
     2221 acgaagatca gcgctcccgg ctggtgcagg tcgtaggtcg agccgaagcc gatgatgccg
     2281 ccctcgttct tcgccaggcg atacacccag ccgcgcgtgg cgcggttgaa cggcatctcc
     2341 tcgcggtcgc cggcgaagaa gtactgcctg aaatactcgc caagctgctc gaagaagtag
     2401 cgcagccgcc cgacgatcgg gtagttgcgc agcacgctgt gcttcttctg cgtgatgtcg
     2461 cggatgaaga cgaacaccag cagcgcggcc accaccagcg cggtcagcgt gccgagcccg
     2521 tacgacagga cgtccttgat catgcccctc ccccgggatc agaacctaaa acttcaggct
     2581 atgaacctaa acttcaggca atgatactcc gcggcgcaac catgctgtcc cggccaatcg
     2641 ggatcacgct cgcaggcgtt tgatgaggcc gaatacctcg gcggcgtggc cgcggggatt
     2701 gacgccgtac gtggcgtggc ggatcacgcc cttcttgtcg atgacgaagg tcgagcgcac
     2761 cagacattcg cgccgcgccc cgtcctgcgt ctccttctcc tgcagcacgc cgtacttgcg
     2821 gcacacctcg ccctccggat cggagagcag cgcgatggtg acgccgttct tgtcgcggaa
     2881 gtcggcgtgc gtgaggcaat cgtcgcgcga cacgccgagc accacgcagt cgtgcttgcc
     2941 gaactggtcc tcgtggtcgg agaactcggt cgtcaccatg gtgcagcccg gcgtgccgtc
     3001 gcgcgggtaa aaatacagta ccacgtgctt cttgcccttg aacgccgaca ggttgacggt
     3061 ctgcatgtcg gcgtcgggaa gctcgaacca cggcgcggct tgtcccggtt gcagcatctg
     3121 cgccgattct attccagatt ttttgaaaga ttgctacata taatcgctcg atgaattcgc
     3181 gattgctggg cggcttgacg tcacgcgaat tcctgcggcg tcattggcag aagcgcccgc
     3241 ttctcgtgcg acgcgcgctg cagggcgcgc tgcggctgct cgacgagcgc gcactaaccg
     3301 cgctggcgag gcgcgatgac gtcgaatccc gcatcgtgga gcgccgcgcc aaccgctggg
     3361 atacggcgca cggcccgttc agaaaaaaat ttcacgccaa gaaaaaaaac tggacccttc
     3421 tggtgagcgg cgtgaacctg cacctcgccg cggcggacgc attgctgcgg cgcttcgact
     3481 tcgtgccgca ggcgaggctc gacgacgtca tggtgagctt cgcgagcccc ggcggcggcg
     3541 tcggcccgca tgtcgattcc tacgatgttt tcctggtgca aggtccggga aggcggcgct
     3601 ggaggatttc cgctcctgga aaatcgaaag tacagacttt cattgcagag ccgggcgatc
     3661 tgctctacct gccgcccggc tggcgccacc acggcgtcgc gctggagccc tgcttcacct
     3721 actcgatcgg ctttcgcgcg ccgcgcggcg cggagctcgg cgccgccttc ctcgactggc
     3781 tgcacgagcg cgggctgccg gacgccgcct accgcgatgc gggcctttca ccagtaaaaa
     3841 acgcggcctt gattccgggc gagatggtgg tattcgtgac gcagctgctg cgccgcatcc
     3901 gctggtcgcg ggccgacgcc cagcggttcc tgggagagta cctcaccctg cccaaggccc
     3961 acgtggtatt cagaccggcg cctgcgccgg gccggccggc gccgcccgcc cgcgggggct
     4021 cgccggtgcg cctcgacccg aagacccgcc tgctgtaccg cggcaaccgg tttttcatga
     4081 acggtgatac tgttaccgtg accgggcgcg atgcgcgctg gctgcgcaac ctcgccgatc
     4141 agaggcgcgc gagcggcgcc ccgtcaggcc gcacgcgaat tgcccggctg ctgcgcagct
     4201 ggcaagagct cggctacctg cgcctcgaaa ggccatgaat ggatgaagcg atctacgagc
     4261 ggttcgacac cagcgccggc ttccaggacg cggtcgaccg gctgcttgag cagccggggc
     4321 gcgagctgcg cgtattcgat cccgacggcg cagcgctgcg gctcaacgat gcgccgcgca
     4381 tcgagggctt cgagcggttt ctcctcgcca gccgcacccg ccggctgtat atggcgttgc
     4441 acgacaccga gtacctcacg cggcgctgcc cccgcatgat ggcgctgctc acacgcttct
     4501 cgcacgcgat ccagatcaac cgcacccacg aggagatccg cgaactgcag gacgcgttcc
     4561 tcgtgctcga cgcggcgcac tacgtgcgcc gcccggtggc ggcgttcttc cgcggcgcca
     4621 tgggcctggg cgacgagacc gacggcctgg cgatgcgctc gcgcttcctg gagatctggg
     4681 cggcatccta ccccgccgtc ccggcgacca cggccgggct gtaggaccgc gcccttctag
     4741 gcctgccgcc agtcgaagcc gtggtcctgg gtagcgaggc cgatccagct ttcgtcgcag
     4801 cccatcgcgc gcgcgagcgc cgcgcgtgcg agctcgggcc ggttattctg ctgcgagaga
     4861 tgggcgccga tcacgtgcct gaggcgcgag cggtcgagcg cgccgagcag ccgctcggaa
     4921 tcacggttgt cgaggtggcc
//
LOCUS       DASPXW010000080         2299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1538283, whole genome shotgun sequence.
ACCESSION   DASPXW010000080 DASPXW010000000
VERSION     DASPXW010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1538283"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1677)
                     /locus_tag="VEL04_01920"
     CDS             complement(<1..1677)
                     /locus_tag="VEL04_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515115.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HYT47155.1"
                     /translation="MTDLSDSAKLAYKAKHKIRFVTAASLFDGHDASINIMRRILQAS
                     GAEVIHLGHNRSVDEIVSAALQEDVQGVAVSSYQGGHLEFFKYMVDLLRSRGGAHIKA
                     FGGGGGVIVPAEISELQEYGVARIYSPEDGQRMGLQGMINDMLARCDFDLSQYAPSEL
                     SENQPRALAQVITALELGVCSFKNSLHEKAKAHPAPVLGITGTGGAGKSSLTDELVRR
                     FRLDQEEALKIAVLSVDPTRRKTGGALLGDRIRMNAIHGENVFMRSLATRDTGREVSA
                     ALGDAIAACKAAGYNLLFVETAGIGQGDAAIVPHVDLSLYVMTPEFGAASQLEKIDML
                     DFADFVAINKFDRRGAEDALRDVRKQVQRNREAFKASPEEMPVYGTIAARFNDDGVTA
                     LYHGIAQKLVEKGLELRKGRLPKPASRVSSSVHVVIPPKRQRYLAEIAECVRGYHAHA
                     AEQSKLGRERQQLMAAKAMLQKAIPEIDALIEKRTKALDASSKELLESWPDTVKAYSG
                     DEQVVRVREREIRTRLTSLSLSGTHLPKVALPKFADHGEILHWRMRENLPG"
     gene            complement(1718..>2299)
                     /locus_tag="VEL04_01925"
     CDS             complement(1718..>2299)
                     /locus_tag="VEL04_01925"
                     /inference="COORDINATES: protein motif:HMM:NF005451.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HYT47156.1"
                     /translation="GLHYREVTASNLVLIDIDGNPVRETQWPINLAGYVIHSAIHAAV
                     ERAHCVMHTHTTNGSAVACLKGGLSHDNFYGAMLKGRVAYHDFEGVTVEPDEKERLVR
                     DIGDKSAVILRHHGLLAWGGSVAEAFLTMWTLQRACDIQVTASRAGEVLPMPPEALNR
                     MVRESSTSEQRTCDDVFAALQRKLDSIDPSYRN"
BASE COUNT          337 a          776 c          756 g          430 t
ORIGIN      
        1 gccgggcagg ttctcgcgca tccgccagtg caggatctcg ccatggtccg cgaacttcgg
       61 cagcgccacc ttcggcaggt gggtgccgga aagcgacagg ctggtgagcc gcgtacgaat
      121 ttcccgctcg cgcacgcgca ccacctgctc gtcgcccgag taggccttga cggtgtcggg
      181 ccagctctcg agcagctctt tcgagctcgc atcgagcgcc ttcgtcctct tttcgatcag
      241 cgcgtcgatt tccggaatcg ccttttgcag catcgctttc gcggccatca gctgctggcg
      301 ctcgcggcca agcttcgact gctccgccgc atgcgcgtga tagccgcgta cgcattccgc
      361 gatctcggcg aggtagcgct ggcgcttggg cgggatgacg acatgcacgc tcgaggagac
      421 ccggctcgcg ggcttcggca accgtccctt gcggagctcg aggccctttt ccaccagctt
      481 ttgcgcgata ccgtgataca gcgccgtgac gccgtcgtcg ttgaagcgcg cggcgatggt
      541 gccgtacacc ggcatttcct ctggcgaggc cttgaacgcc tcgcggtttc tctgtacttg
      601 cttgcggaca tcgcgcagcg cgtcctcggc gcccctgcgg tcgaacttgt tgatggcgac
      661 gaaatccgcg aagtcgagca tgtcgatctt ctcgagctgg ctcgcggcgc cgaattccgg
      721 cgtcatcacg tacagcgaca ggtcgacgtg cggcacgatc gcggcgtcgc cctggccgat
      781 gcccgcggtc tccacgaaca ggaggttata gcctgccgcc ttgcaggcgg cgatggcgtc
      841 gcccagcgcc gccgacacct cgcggccggt atcgcgcgtc gccagcgagc gcatgaagac
      901 gttctcgccg tggatcgcgt tcatgcggat gcggtcgccg agcagcgcgc cgccggtttt
      961 cctgcgcgtc gggtcgaccg aaaggacggc aattttcagc gcctcttcct gatccagccg
     1021 gaagcggcgc accagctcgt cggtgagcga gctcttgccc gcgcccccgg tgccggtgat
     1081 tcccagaacc ggggccgggt gtgccttcgc cttttcatga agggagtttt tgaaactgca
     1141 aacccccaat tccagggcgg tgatgacctg cgccagggcg cgcggctggt tctcgctcag
     1201 ctcgctgggc gcgtactgcg agagatcgaa gtcgcagcgc gccagcatgt cgttgatcat
     1261 gccctgcagg cccatcctct gcccgtcctc gggcgagtag atgcgcgcga cgccgtactc
     1321 ctgcagctcg ctgatctcgg cgggcacgat cacgccgccg ccgccgccga acgccttgat
     1381 gtgcgcaccg ccccggctgc gcagcagatc gaccatgtac ttgaagaact cgaggtgccc
     1441 gccctgatag gaggacaccg cgaccccctg cacgtcctcc tgcagcgccg cgctcacgat
     1501 ttcgtcgacc gagcggttgt ggccgaggtg gatcacctcg gcgcccgagg cctgcaggat
     1561 cctgcgcatg atgttgatcg acgcgtcgtg gccgtcgaac aggctcgccg cggtgacgaa
     1621 acggatcttg tgcttcgcct tgtaggcgag cttcgccgag tcggagagat ccgtcatccg
     1681 gcgaggctac ttgacgttta cgtcaacgtc aacttgctca gttccggtac gacggatcga
     1741 tcgaatcgag cttgcgctgg agcgccgcga agacgtcgtc gcaggtgcgc tgctcgctgg
     1801 tgctcgactc acgcaccatg cggttcaacg cctcgggcgg catcggcagc acctcgccgg
     1861 cgcggctcgc ggtcacctgg atgtcgcagg cgcgctgcag cgtccacatg gtgaggaagg
     1921 cctcggccac gctgccgccc caggcaagca ggccgtggtg gcgcaggatc accgccgact
     1981 tgtcgccgat gtcgcgcact aggcgctcct tctcgtccgg ctcgacggtg acgccctcga
     2041 agtcgtggta ggcgacgcgc cccttcagca tcgcgccata gaagttgtcg tgcgaaaggc
     2101 cgcccttcag gcaggcgacc gcgctgccgt tggtggtgtg cgtgtgcatc acgcagtggg
     2161 cgcgctcgac cgcggcgtgg atcgcggagt ggatgacgta gccggcgagg ttgatcggcc
     2221 actgcgtctc gcgcaccggg ttgccgtcga tgtcgatcag cacgaggttc gatgccgtca
     2281 cctcgcggta gtgcaggcc
//
LOCUS       DASPXW010000081         3621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1538503, whole genome shotgun sequence.
ACCESSION   DASPXW010000081 DASPXW010000000
VERSION     DASPXW010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1538503"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="VEL04_01930"
     CDS             <1..858
                     /locus_tag="VEL04_01930"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYT47157.1"
                     /translation="PVISFGAGGVAVEAVRDTAIALPPLNAALARELMARTRVHRLLV
                     GYRNVPPADLEALAAVLCGVSRMVCMLPWLKEMDLNPLLAHPGGAVIADARVVIDPAQ
                     PPRAGPHYPHMAIHPYPVELEGEIALRDRRKVKVRPIRPEDAELEQRFFNGLSERSRY
                     QRFMERMRELPPNLLARFTQLDYDRELALVALHPENEDFIAVGRYAPNPDGVSAEFAL
                     TVADAWQGKGVGRALLERLCDAARNAGYKALYGYILDANREMLDLAERLGFVQSSRSG
                     HEVSVVRRL"
     gene            925..1368
                     /locus_tag="VEL04_01935"
     CDS             925..1368
                     /locus_tag="VEL04_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HYT47158.1"
                     /translation="MFKHILIPTDGSEVAAKAIRAGVALAKEMGARVTGYHAQEPLPT
                     HIYGEGYVADKQLVAEFERRAGEFAERCVGAVAVAAKAAGVAFEPLVAKSPVPYKGIV
                     DAAKKQKCDAIFIASHGHRGLTRLLLGSVTQEVLTHSKIPVLVFR"
     gene            1468..1890
                     /locus_tag="VEL04_01940"
     CDS             1468..1890
                     /locus_tag="VEL04_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004190903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HYT47159.1"
                     /translation="MKALKGTRTHDNLKAAFAGESQANRRYLYFAAKADVEGQNDVSA
                     LFRSTAEGETGHAHGHLEYLEAVGDPATNLPIGRTRDNLKAAVAGETHEYTDMYPGMA
                     KQARDEGFGEIADWFETLAKAERSHANRFQKALDGLSD"
     gene            1890..3215
                     /locus_tag="VEL04_01945"
     CDS             1890..3215
                     /locus_tag="VEL04_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HYT47160.1"
                     /translation="MREGSLEAPTRHPLDWKSAEFKDPAKIEKELGRIFEICHGCRRC
                     VNLCTTFPTLFDLVDASPTLEVDGVKQDDYWKVVDQCYLCDLCYMTKCPYVPPHPWNV
                     DFPHVMLRAKSFKFDQGTRFRDRLLSSTDAIGKLAAIPLVAQTVNAVNKNPLARSALQ
                     TVLGVHKDRELPPYAGKKFRSHAVASKPFPVKDGKNTPGKVAIFATCYANYNEPGMAH
                     DLLAILEHNEIPYVLAEKEVCCGMPKLELGDLEGVDALKQKNMPVLAKLAREGYAILA
                     PVPSCALMFKQELPLMYPDDADTLAVRDAMFDPFEYFVLRKKEGLLKSDFKQPLGKIS
                     YHIPCHSRVQNMGQKTREMLESVPGTEVTVVERCSGHDGTWGVKTEHFDNSMKIGRPV
                     FRQMAAAAPDYVSSDCPIAARHIMQGMGEEAGNAVKAHPLSLLRKAYGI"
     gene            3223..>3621
                     /locus_tag="VEL04_01950"
     CDS             3223..>3621
                     /locus_tag="VEL04_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47161.1"
                     /translation="MLLFLLAGCAAQLPAGGVAFVLMGDTPYSASEVERLDLAIEELN
                     GQDPAFIAHVGDITSGRGPCTDEWFEARKRQFARLRHPFVLLPGDNDWTDCHRSGFDP
                     VERLGKWRMLFCVQVVVQDFERQNGAYCEHV"
BASE COUNT          676 a         1198 c         1144 g          603 t
ORIGIN      
        1 ccggtgatct cgttcggcgc cggcggcgtc gcggtcgaag cggtgcgcga caccgcgatc
       61 gcgctgccgc cgctcaacgc cgcgctcgcc cgcgagctga tggcgcgcac gcgcgtgcac
      121 cggctgctcg tcggctatcg caacgtcccg cccgccgacc tggaggcgct cgccgcggtg
      181 ctctgcggcg tgtcgcgcat ggtctgcatg cttccctggc tcaaggagat ggacctcaat
      241 ccgctgctcg cgcatccggg cggcgccgtc atcgccgacg cccgcgtcgt gattgaccct
      301 gcgcagccgc cgcgcgcagg cccgcattac ccccatatgg cgattcaccc ctatccggtc
      361 gagctcgaag gcgagatcgc cctgcgcgac cgccgcaagg tgaaagtgcg gccgatccgc
      421 ccggaggatg ccgagctcga gcagcgcttc ttcaacggcc tgtcggagcg ctcgcgctac
      481 cagcgcttca tggagcgcat gcgtgagctg ccgccgaatc tgcttgcccg gtttacccag
      541 ctcgactacg accgcgagct cgccctcgta gctttgcatc ccgaaaacga ggacttcatc
      601 gccgtcgggc gctacgcccc caatcccgac ggcgtgagcg ccgagttcgc tctcaccgtg
      661 gccgacgcgt ggcaaggcaa aggggtgggc cgcgcgctcc tcgagcgcct gtgcgatgcg
      721 gccaggaacg cgggctacaa ggcgctctat ggctacattc tcgatgccaa ccgggagatg
      781 ctcgacctcg cggagcgcct cggcttcgtc cagagctcgc gtagcggcca cgaggtttcg
      841 gtcgtgcgcc gcctttgatc tgcgtcaaaa cgccgtcctc gcggctgtct aacctgaagc
      901 tgttcaatta ccgaggaaag cgccatgttc aagcacatcc tgatacccac cgacggctcg
      961 gaggtcgcgg ccaaggccat ccgcgcgggc gtcgcgctcg ccaaggaaat gggcgccagg
     1021 gtgaccggct atcacgccca ggagcccttg ccgacgcata tctatggcga aggctatgtc
     1081 gccgacaagc agctggtcgc ggagttcgag cgccgcgccg gtgagttcgc cgagcgctgt
     1141 gttggagcgg tcgccgtggc agcgaaggcc gcaggagtcg cgttcgagcc gctggtcgcc
     1201 aagtcgccgg ttccttacaa gggcatcgtc gacgccgcga agaagcaaaa gtgcgacgcc
     1261 atcttcatcg cctcgcacgg tcaccgcggc ctcacgcgcc tgctgctcgg cagcgtgact
     1321 caggaagtgc tgacccactc caagatcccg gtgctggtgt tccgctagcg ttccattagg
     1381 cgccatcaat cgacaagatc atgattttcc atttgatcta ttgaacgtag gcgcttacgc
     1441 tctgtctccc acccgaagga gactgccatg aaagcgctca aaggcaccag gacgcacgac
     1501 aatctcaagg ccgcgttcgc cggcgaatcg caggccaacc gcagatacct ctactttgct
     1561 gcgaaggcag atgtagaagg ccagaatgac gtttcagccc tgttccgctc gaccgccgag
     1621 ggcgagaccg ggcacgcgca cgggcacctc gagtatctcg aggcggtggg cgacccggcg
     1681 accaatctgc cgatcgggcg cacgcgcgac aacctgaagg cggcggtggc cggcgagacc
     1741 cacgagtaca ccgacatgta ccccggcatg gcgaagcagg cgcgcgacga gggcttcggc
     1801 gagatcgccg actggttcga aacgctggcg aaggccgaga gaagccacgc caaccgcttc
     1861 cagaaggcac ttgacgggct cagtgactga tgcgcgaagg ttcgctcgaa gccccgaccc
     1921 ggcatccgct cgactggaag agcgccgagt tcaaggaccc ggcgaagatc gagaaggagc
     1981 tcgggcggat cttcgagatc tgccacggct gcaggcggtg cgtgaacctg tgcaccacct
     2041 tcccgacgct gttcgacctg gtcgacgcct cgccgacgct ggaggtcgac ggcgtcaagc
     2101 aggacgacta ctggaaggtg gtcgaccagt gctacctctg cgacctgtgc tacatgacga
     2161 agtgcccgta cgttccgccg catccgtgga acgtcgattt cccgcacgtc atgctgcgcg
     2221 cgaaatcgtt caagttcgac caagggaccc ggtttcgcga ccggctgctc tcctcgaccg
     2281 acgccatagg caagctcgcc gcgatcccgc tggtcgcaca gaccgtcaat gcggtcaaca
     2341 agaatccgct cgcgcgcagc gcgctgcaaa ctgtccttgg agtacataag gaccgggagc
     2401 ttccgcctta cgcgggcaaa aaattcagaa gccatgccgt cgcgtccaag ccttttcccg
     2461 taaaggacgg caagaacact cccggcaagg tcgcgatctt cgccacctgc tacgccaact
     2521 acaacgagcc agggatggcg cacgacctgc tcgccatcct cgagcacaac gagatcccct
     2581 acgtcctggc ggaaaaagag gtgtgctgcg gcatgccgaa gctcgagctg ggggatctcg
     2641 aaggggttga tgccctgaag cagaagaaca tgccggtgct ggcgaagctg gcgcgcgagg
     2701 gctacgccat cctggcgccc gtgccttcgt gcgcgctgat gttcaagcag gagctgccgc
     2761 tcatgtaccc ggacgacgcc gacaccctgg cggtgcgcga tgccatgttc gatccgttcg
     2821 aatattttgt tttaagaaaa aaagaaggcc tgttgaaaag cgacttcaag caaccactcg
     2881 gaaaaatctc ctatcacatc ccgtgccact cccgcgtgca gaacatgggc cagaagacgc
     2941 gcgagatgct ggagagcgtg ccgggcaccg aggtgacggt ggtcgagcgc tgctcgggtc
     3001 acgacggcac ctggggcgtg aagaccgagc acttcgacaa ctcgatgaag atcggccggc
     3061 cggtgttccg ccagatggcc gccgccgcgc ccgactacgt cagctccgat tgcccgatcg
     3121 cggcaagaca catcatgcag ggaatgggag aggaagccgg taatgcagtg aaagcgcatc
     3181 ccctgtctct cctgcgcaag gcttacggca tctagggccc tattgctgct gtttctgctg
     3241 gctggctgcg cagcccagct gcccgccggc ggcgtcgcct ttgtcctgat gggcgatacg
     3301 ccgtactccg catccgaggt cgagcgcctg gacctcgcga tcgaggagct gaacggccag
     3361 gacccggcgt tcatcgcgca cgttggcgac atcacctcgg ggcgcgggcc ctgcaccgac
     3421 gagtggttcg aggcgcgcaa gcgccagttc gcgcggctgc ggcacccctt cgtcctgctg
     3481 cccggcgaca acgactggac cgactgccac cgcagcggct tcgacccggt ggaaagactg
     3541 gggaaatggc gcatgctttt ctgcgttcag gtggtagttc aggattttga aagacagaac
     3601 ggggcctact gcgaacacgt c
//
LOCUS       DASPXW010000082         2081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_782754, whole genome shotgun sequence.
ACCESSION   DASPXW010000082 DASPXW010000000
VERSION     DASPXW010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782754"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..980
                     /locus_tag="VEL04_01955"
     CDS             <1..980
                     /locus_tag="VEL04_01955"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYT47162.1"
                     /translation="HSMGGAIVLAYAAAYPRRVRRLIAVDAAGVLQASVYAEFLGRAT
                     AGRAVGMEMRWFDSLVRTIQDRAERLPGRSELILENAEARRRLLRGEPTAIAGLALAE
                     HDLSRALRSIAAPTLVIWGGNDKVAPLRTGQAVAGVIPGARLAVIPGAGHEPQLEAPQ
                     RFNAIVLDELGGRLEAKPYALPRAKIQGGRSARCAGERGQEFSGDYERLILESCLEVQ
                     IADARIGYLRATSSTVRIVNSQIRDGIGASSSRLELTAGVVGGPLMLYATSVDAAGTR
                     FESDDVIAANRGSAPLTLRLSVTELSRAGAAPRYVHDIIRLAPGQRLAD"
     gene            complement(1159..>2081)
                     /locus_tag="VEL04_01960"
     CDS             complement(1159..>2081)
                     /locus_tag="VEL04_01960"
                     /inference="COORDINATES: protein motif:HMM:NF019285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HYT47163.1"
                     /translation="ILAFRALPLVLVVSALSALLFYWRVLPAVVRAISWLLERSMRVG
                     GVVGLSTAANVFVGMVEAPLFVRPYLGRVSRGELFAIMVGGMASIAGTVLFLYGAILG
                     PVLPDAVAHLLIASILSAPAALVIAFVMVPPHAATGGDIVFRSEASSSVDALTRGTLD
                     GAQLLLNIVAMLVVFVALVALVNFAIAPYTLQGMLGWALAPLAWLAGVPWSEARAAGA
                     LLGTKTVINELVAYVELAKMSELSERSRVLMTYALCGFANFGSLGIMLGGLGTMCPER
                     RGEVVALGMKSIVAGTLATCLTAATVALIF"
BASE COUNT          368 a          723 c          729 g          261 t
ORIGIN      
        1 gtcattcgat gggcggcgcc atcgtgctcg cctatgcggc ggcctacccc cggcgcgtgc
       61 gccgcctgat cgcggtggac gccgccggcg tgctgcaggc ctcggtgtac gccgagtttc
      121 tcggccgcgc cacggcggga cgcgccgtcg gcatggaaat gcgctggttc gattcgctgg
      181 ttcgcaccat ccaggatcgg gccgagaggc tgcccggcag gagcgagctg attctcgaaa
      241 acgcggaggc gcgccggcgg ctgctgcgcg gcgagcccac ggccatcgcc ggcctggcgc
      301 tcgccgagca cgatctcagc cgggccttgc gcagcatcgc cgcgcccacc ctggtgatct
      361 ggggcggcaa cgacaaagtc gcgccgctgc gcaccgggca ggcggtggcc ggcgtcattc
      421 cgggagcgcg gctggcggtg attccgggcg cgggccacga gccgcagctg gaggcgccgc
      481 agcgcttcaa tgccatcgtg ctcgacgagc tgggcgggcg gctcgaggcg aaaccctatg
      541 ccctgccaag ggcaaagatc cagggaggcc gcagcgcgcg ctgcgcgggc gagcgcgggc
      601 aggagttcag cggcgactac gagaggctga tcctggagag ctgcctggag gtgcagatcg
      661 ccgatgcccg catcggctac ctgcgcgcca ccagctccac ggtgcgcatc gtgaattcgc
      721 agatccgcga cggcatcggc gcgagcagct cgcgcctgga gctcaccgca ggcgtcgtcg
      781 gcggccccct catgctctac gccaccagcg tggacgccgc ggggacgcgc ttcgaatcgg
      841 acgatgtcat cgccgccaac cgcggcagcg cgccgctcac cctgcgtctt tcagtgaccg
      901 agctgtctcg cgccggcgcg gcgccgcgtt acgtgcacga catcatccgg ctggcgccgg
      961 gccagaggct tgcggactag cgtgcgggca gaaaaaaaac cccggtaggg ggaccggggt
     1021 cggttacctg catacgcaca caaaatggga gggaggcgcg tatgcaaggg gggaggctcg
     1081 atgctagaag cccgctccgg ccgtggctat ccccgaaagc gtgatcctga tgtagtggac
     1141 cagctacagc catctcgcct agaaaatcaa cgcgaccgtc gcggcggtga ggcaggtggc
     1201 gagcgttccc gcgacgatcg acttcatgcc gagcgccacc acctcgccgc gccgctccgg
     1261 gcacatggtg ccgagcccgc cgagcatgat gccgaggctg ccgaaattgg cgaagccgca
     1321 cagcgcgtag gtcatcagca cgcggctgcg ctccgagagc tcggacattt tcgccagctc
     1381 gacgtaggcg acgagctcgt tgatcacggt cttggtgccg agcagcgcgc ccgccgcgcg
     1441 cgcttcgctc cagggcaccc cggcgagcca ggcgagcggc gcgagcgccc agccgagcat
     1501 gccctgcagc gtgtagggcg cgatggcgaa attcaccagc gcgaccaggg cgacgaacac
     1561 caccagcatc gccacgatgt tgaggagcag ctgcgcgccg tcgagcgtgc cgcgcgtcag
     1621 cgcatcgacg ctgctcgagg cttcggatcg aaacacgatg tccccgcccg ttgcggcatg
     1681 aggcggaacc atcacaaacg cgatcaccag cgccgcgggg gccgacagga tcgaggcgat
     1741 gagaagatgc gcgaccgcat ccggcagcac cggtccgaga atcgcgccgt agagaaacag
     1801 caccgtcccg gcgatcgagg ccatgccgcc caccatgatg gcgaagagct cgccgcgcga
     1861 cacgcggccg agatagggcc gcacgaacag cggcgcctcg accatgccga cgaacacgtt
     1921 cgccgcggtg gaaagcccga ccacgccgcc gacgcgcatc gatctttcga gcagccacga
     1981 aatggcgcgc accaccgccg gcagcacccg ccagtagaac agcagcgcgg aaagcgcgct
     2041 caccaccagc accagcggca gcgcgcggaa agcgaggatg a
//
LOCUS       DASPXW010000083         2909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1538613, whole genome shotgun sequence.
ACCESSION   DASPXW010000083 DASPXW010000000
VERSION     DASPXW010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1538613"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VEL04_01965"
     CDS             complement(<1..300)
                     /locus_tag="VEL04_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HYT47164.1"
                     /translation="MSDKASSGEKLLYCSFCGKSQHEVRKLIAGPSVFICDECIELCN
                     DIIREETASDKGSKGAKSDLPTPREIRQILDQYVIGQELAKKILSVAVYNHYKRLE"
     gene            complement(304..930)
                     /gene="clpP"
                     /locus_tag="VEL04_01970"
     CDS             complement(304..930)
                     /gene="clpP"
                     /locus_tag="VEL04_01970"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HYT47165.1"
                     /translation="MKWQQSDPDVKDLGLIPMVIEQSGRGERAYDIYSRLLKERVVFL
                     VGPVTEITANLIVAQLLFLESENPDKDIFFYINSPGGSVSAGLAIYDTMQFIKPDVST
                     LCVGQAASMGALLLAAGDKDKRFCLPNSRVMIHQPMGGFQGQATDIEIHAKEILYLRS
                     RLNEILAKHTGKPIDVIAHDTERDFFLGAEDSVKYGLVDKVLVSRENT"
     gene            complement(927..2228)
                     /gene="tig"
                     /locus_tag="VEL04_01975"
     CDS             complement(927..2228)
                     /gene="tig"
                     /locus_tag="VEL04_01975"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HYT47166.1"
                     /translation="MANVETLGSLERRVSMTLPISEIDRQVDERLKKIARSVRMPGFR
                     PGKVPLKLVAQTYGPQVRSEVLGDAVQKAFSDAVKEAKLKVAGYPRIEKKDGANEKSL
                     EFSATFEVYPEVKLGDISAASIERPQVAVDDAALDRTIEILRRQRTRFAAAGRPARDG
                     DRLTVDFHGTIDAQPFEGGKAENFPFVLGEGRMLPEFEAAARGMLEGESKSFELRFPD
                     DYHGKEVAGKKACFNLSLKKIEQPQLPELDAEFAKQLGVADGDVAKMRSEIRANVERE
                     VAKRVDARVKAQALQTLLDATPIELPKSLVNLESQQLVERAGADLQARGIKPGQVPLN
                     AASFEDTAKRRVALGLVIGELARAENLQPKPAEVRALVEQEAQSYESPAEVVKWFYMQ
                     PQRLSEIEGVALEANVVKWVLSKAKVVDKQVPFDELMGSAA"
     gene            complement(2264..2348)
                     /locus_tag="VEL04_01980"
     tRNA            complement(2264..2348)
                     /locus_tag="VEL04_01980"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2312..2314),aa:Leu,seq:tag)
     gene            2401..>2909
                     /locus_tag="VEL04_01985"
     CDS             2401..>2909
                     /locus_tag="VEL04_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HYT47167.1"
                     /translation="MPVGHYENFPVASLLLPAPLRAPIEVIYRFARSADDIADEGNDA
                     ADMRLAKLSVYQARLAKIAGGETPDDPLFRDVAGIVRSHRLPLQLFRDLLDAFAQDVT
                     KKRYASFAELLDYCRRSANPVGRLLLHLFKRTTDSDLARSDAICSALQLINFWQDIEI
                     DYAKGRIYLP"
BASE COUNT          476 a          957 c          882 g          594 t
ORIGIN      
        1 ttcgagccgc ttgtagtggt tgtagacggc gaccgaaagg atcttcttcg cgagctcctg
       61 gccgatcacg tactggtcga ggatctgccg gatctcgcgc ggcgtcggca ggtcggactt
      121 cgcgcccttg ctgcccttgt cggaggccgt ctcctcgcga atgatgtcgt tgcacagctc
      181 gatgcactcg tcgcagatga atacagacgg accggcaatg agctttctta cctcgtgctg
      241 gctcttgccg cagaacgagc agtacagcag cttctcgccc gaggacgcct tgtcagacat
      301 cgcctaggtg ttctcccttg ataccagcac tttgtccacc agcccgtact ttaccgaatc
      361 ttcggccccc aggaaaaagt cgcgctcggt gtcgtgggcg atgacgtcga tcggcttgcc
      421 cgtgtgcttg gccaggatct cgttcaggcg actgcgcagg tacaggatct ccttggcatg
      481 gatctcgatg tcggtggcct ggccctggaa cccgcccatc ggctggtgga tcatgacgcg
      541 cgagttgggc aggcagaagc gcttgtcctt gtcgccggcg gcgagcagca gcgcgcccat
      601 gctggccgcc tggccgacac aaagggttga gacgtcgggt ttgatgaact gcatggtgtc
      661 gtagatcgcg aggccggccg acaccgagcc gccgggcgag ttgatgtaga agaagatgtc
      721 cttgtcgggg ttctccgact cgaggaacag cagctgggcg acgatcaggt tggcggtgat
      781 ctcggtcacc ggcccgacca ggaacaccac gcgctctttc agcagccgcg aatagatgtc
      841 gtaggcgcgc tcgccgcgcc ccgactgctc gatgaccatc gggatcagcc cgaggtcttt
      901 cacatcagga tctgattgtt gccatttcat gccgcgctcc ccatcagttc atcgaagggg
      961 acctgcttgt cgacgacctt cgctttggac aaaacccact tgacgacgtt ggcttccagg
     1021 gccactcctt cgatctcgga caagcgctgc ggctgcatgt agaaccattt tactacctcg
     1081 gcgggactct cgtagctctg cgcttcctgc tccaccagcg ctctgacttc ggcgggcttg
     1141 ggctgcagat tttccgcgcg cgccagctcg ccgatcacca ggccgagcgc gacgcggcgc
     1201 ttcgccgtat cctcgaacga cgcggcattg agcggcacct gcccgggctt gatgccgcgc
     1261 gcctgcaggt cggcgcccgc ccgctcgacg agctgctgcg actccaggtt gaccagcgac
     1321 ttcggcagct cgatcggcgt agcgtcgagc agcgtctgga gcgcctgcgc cttcacccgg
     1381 gcgtcgacgc gctttgccac ctcgcgctcg acgttggcgc gaatctcgct ccgcatcttc
     1441 gcgacgtcgc cgtcggcgac gccgagctgc ttggcaaatt cggcgtcgag ctcgggcaac
     1501 tgcggttgtt cgattttttt cagtgaaagg ttgaaacaag ccttctttcc ggcgacttcc
     1561 ttgccgtggt agtcatcggg aaacctgagc tcgaagctct tcgactcgcc ctccaacatg
     1621 ccgcgcgcgg ccgcctcgaa ctcgggcagc atgcggccct cgccgagcac gaacgggaag
     1681 ttctcggcct tgccgccttc gaagggctga gcgtcgatcg tgccgtggaa atcgaccgtc
     1741 aggcggtcgc cgtcgcgcgc cggccggcct gccgcggcga agcgcgtgcg ttgcctgcgc
     1801 aggatctcga tggtcctgtc gagcgccgcg tcgtcgaccg cgacctgcgg gcgctcgatg
     1861 ctcgccgcgc tgatgtcgcc gagcttcact tccgggtaga cctcgaaggt ggcgctgaac
     1921 tccaatgatt tttcatttgc accatctttc ttctcaatcc gcgggtagcc ggcgaccttg
     1981 agcttcgctt ccttcaccgc gtcgctgaat gctttctgca ccgcgtcgcc gaggacttcc
     2041 gagcgcacct gcggcccgta ggtctgcgcg acgagcttga gcggcacctt gccggggcga
     2101 aagccgggca tgcgcacgct gcgcgcgatc ttcttcaggc gctcgtcgac ctgccggtcg
     2161 atctcggaga tgggcagggt catcgacacg cgccgctcga gcgatcccag ggtttccacg
     2221 ttggccattc ccttctagtt cctgtctgcg ctcttcatgt cggtggtgcg aagggaggga
     2281 ctcgaaccca catgcctcgc ggcgccggct cctaaggccg gtgcgtctac caattccgcc
     2341 accctcgccg cagtcgcgtg cacaaaaaag cgggataaaa acaacgtatt atacccgcgc
     2401 gtgccggtcg gacattacga gaacttcccc gtcgcctcgc tgctgttgcc ggcgccgctg
     2461 cgcgctccaa tcgaagtcat ttaccgcttt gcgcgcagcg cggacgacat cgccgacgag
     2521 ggaaacgacg ctgccgatat gcggcttgca aaattgagcg tctatcaggc gaggctggcg
     2581 aaaattgcag gcggcgaaac gcccgacgac ccgctatttc gcgacgtggc gggcatcgtt
     2641 cgcagccatc ggcttccgct ccagcttttc agggacctgc tcgacgcctt tgcgcaggat
     2701 gtgacgaaaa aacgctacgc aagcttcgcc gagctgctcg actactgccg ccgctcggcg
     2761 aacccggtcg gccgcctgtt gctgcatctt ttcaaacgaa cgactgactc agacctggct
     2821 cgatcggatg ccatctgctc ggcgctgcaa ctcatcaact tctggcagga catcgagatc
     2881 gactacgcca agggccgcat ttacctccc
//
LOCUS       DASPXW010000084         4944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1414777, whole genome shotgun sequence.
ACCESSION   DASPXW010000084 DASPXW010000000
VERSION     DASPXW010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4944
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414777"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="VEL04_01990"
     CDS             <1..846
                     /locus_tag="VEL04_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011400980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HYT47168.1"
                     /translation="FYIYYSMFGFQRVGDLIWAAADSRARGFLVGGTAGRTTLAGEGL
                     QHQDGSSHLTASTVPNCRAYDPCFAYELAAIVQEGARRMLDAQEDEFYYLTVGNENYP
                     HPAMPPGAGEGILKGMYLLKKADQPALQLLGSGAILREVIAAAELLEKDWGVAANVWS
                     VTSFTELRRDGMQAARARRFGGTAASWVEECLKNTQGPVIAASDYVSAVADLIRPYAP
                     GKFVALGTDGFGRSDTRAALRAFFEVDARNIAVAALAALQSPSTAEALNRYGIDCVSR
                     PPWER"
     gene            complement(1134..2099)
                     /locus_tag="VEL04_01995"
     CDS             complement(1134..2099)
                     /locus_tag="VEL04_01995"
                     /inference="COORDINATES: protein motif:HMM:NF014042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase CofD family
                     protein"
                     /protein_id="HYT47169.1"
                     /translation="MGSVVALAGQVGAAKLADGLYRLRGKNLAVIVNTGDDYEHLGLG
                     FSPDLDTMLYTLAGIASPAAGWEPEGESHAVHGMLKSLGGPERPQLGDRSLAAPLLRS
                     DGLANDRRLTQITLDFCRGLGVAARILPMSDDAVRTHVLTDDGAVSFHEYFLQLGCEP
                     AVRSFQYAGADEARISDEVLDALHAADLEAVVICPANPYHVIRPILEVRGMKQLLRKR
                     GAPVIAVTPIVGGKALKGTAGKMMRELGREPSVRSVAGEYLHFIDGFVIDRVDESAAE
                     SIRSLGIQVLATAAVMRSIDDRTALARAVLEFAGSIREARALEVR"
     gene            complement(2116..2793)
                     /locus_tag="VEL04_02000"
     CDS             complement(2116..2793)
                     /locus_tag="VEL04_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYT47170.1"
                     /translation="MSATGVALIGGAGEGLGAALGRRFTQGGYEVVLASRSRDGPGKV
                     DLREEAEVVALFDRVEKRAPVEVAVFNAGANFRASILDTPAEMFEKVWRLGCYAGFLF
                     GREAARRMAPRGKGSILFTGATASLRGAAQFAAFAAAKGGLRQVAQSMARELGPKNIH
                     VAHVVIDGMIDNPRTRSRMAERVKDLPPDGMLATDAIAELFWQLHAQPRSAWTFEADV
                     RPWAEKF"
     gene            complement(2803..3651)
                     /locus_tag="VEL04_02005"
     CDS             complement(2803..3651)
                     /locus_tag="VEL04_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYT47171.1"
                     /translation="MTLRDSTRLVLLAAIWGASFLFNRITAPLLGPVLTAELRTLIAG
                     IALAIWFAATGFNPEWRRWGRQYVIVGMLTSGLPFLLWAYAALSLTAGLMSVLNATSP
                     MWGALWSALLLGERLNARRVLGLVIGVVGVALVTRPEPGATLVYPAIIAALAAAFCYG
                     LAGAYMKRWASGVPSRGMAVGTQLAAGVLLIPFIALWPPAAAPTPLVAASMLALGLVC
                     GAVAYLLYFRLVTDIGPTRALTVTYLIPIFGVCWGALFLGETISLSMLAGAALVVLGT
                     VCVLRR"
     gene            3688..>4944
                     /gene="soxC"
                     /locus_tag="VEL04_02010"
     CDS             3688..>4944
                     /gene="soxC"
                     /locus_tag="VEL04_02010"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR04555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HYT47172.1"
                     /translation="MENMLPVAGNGLLDRRAFLRGGAALAAVMTGYTVARPAAAAPLA
                     DEPWSLAPGAISPAYEKRSRFEEKVARTLSNPKGETRTSHARTPHHLINGSFTPNGLH
                     FTIVHSGTPDIDPDRHRLVIHGLVKRPLAFSLDALGRYPMVSRMTFVECGGNSAPLFS
                     PEPVQASVQALHGLASCAEWTGVMLSSLLEECGVEPKARWLIAEGADSLALSRSVPLG
                     KALDDAMVALYQNGERLMPGNGYPMRLLLPGWEGNMNVKYLRRIKLVEQPAMSYYEAR
                     TYSQILPGGKAYKFYFVQEVKSFVTHPSPGLKLNGPGFYEVSGIAYSGNGRISKVMVS
                     ADGGRSWAQAALQEPVLSKAFTRFRVPWRWDGGPAVLQSRAWDEAGYAQPTRAQFVAV
                     RGEASSVPPVTAFPNQHFNSITSWAVD"
BASE COUNT          844 a         1683 c         1687 g          730 t
ORIGIN      
        1 ttctacatct attactcgat gttcggtttc cagcgcgtcg gcgacctgat ctgggctgcg
       61 gccgactcgc gcgcgcgcgg cttcctggtc ggcggcacgg cggggcgcac cacgcttgcc
      121 ggcgaaggcc tgcagcacca ggacggctcc tcgcatctca ccgcgtcgac ggtgcccaac
      181 tgccgcgctt acgatccgtg cttcgcctac gagcttgcgg cgatcgtcca ggagggcgcg
      241 cgacgcatgc tcgatgccca ggaggatgag ttctactacc tcacggtcgg caacgaaaac
      301 tatccgcacc cggcaatgcc cccgggagcc ggggaaggca tcctgaaagg gatgtacctg
      361 ctcaagaagg ccgaccagcc ggcgctgcag ctgctcggca gcggagcgat cctgcgcgag
      421 gtgatcgcag cagcggagct gctcgagaag gactggggcg tcgcggcgaa tgtctggagc
      481 gtcacctcgt tcaccgagct gcggcgcgac ggcatgcagg ccgcccgcgc acggcggttt
      541 ggcggtacgg ccgcgagctg ggtggaagag tgcttgaaaa atactcaagg tccggtgatc
      601 gcggcaagcg actatgtcag cgcggtcgcc gacctcatcc gtccctacgc gccggggaag
      661 ttcgttgcgc taggcaccga cggcttcggc cgcagcgaca cgcgcgcagc gctgcgcgcg
      721 ttcttcgagg tcgatgcgcg caacatcgca gtcgcggcgc tcgccgcgct gcaaagcccg
      781 tcaaccgccg aagccttgaa ccggtatggg atcgactgcg tgagccggcc cccctgggag
      841 cggtagcggc gcccggaatc cggggtcaga gcccgatttc tggatggggg gagaaaaacg
      901 caggacgaag gcgtcgcgct cgcgtgccat gcggccgcgc tggtaggaaa gcggccgcgc
      961 ggtgcccagt tcgagaagtt gacgttgatg aagagcctcg agtgaatgtc cctggagccg
     1021 agcggcaaat tccgccgact gctgatcgcg ctggataagg gcgcgctgca cctcgcgctc
     1081 gtcgcctgct gccgcgccgg cgatcgatac gaggatcgtc atgagaacag ctctcaccgc
     1141 acctccagcg cgcgcgcctc gcggatcgag ccggcgaact cgagcacggc acgtgcgagc
     1201 gcagtccggt catcgatgga gcgcatcacg gcagccgttg ctaggacctg gatgccgagc
     1261 gaacgtatgc tctcggcggc tgactcgtcc acgcggtcga tgacgaagcc gtcgatgaag
     1321 tgcaggtatt ccccggcgac cgagcgcacc gagggctcgc ggccgagctc gcgcatcatt
     1381 tttccagccg tgcctttaag cgccttgccg ccgacgatcg gcgtcacggc gatcaccggc
     1441 gcgccgcgct tgcgcagcag ctgcttcatg ccgcgcacct ccaggatcgg gcggatgacg
     1501 tggtacgggt tggcgggaca gatcaccacc gcctcgagat cggctgcgtg cagcgcgtcg
     1561 agcacctcgt cggaaatgcg cgcttcgtcg gcgcccgcat attgaaagct gcgcacggcc
     1621 ggctcgcaac cgagctgcag gaagtattcg tggaaggaaa ccgcgccgtc atcggtgagc
     1681 acgtgggtgc gcaccgcatc gtcgctcatc ggcaggatgc gcgccgcgac gccgaggccg
     1741 cggcagaaat cgagcgtgat ctgcgtcagg cgccggtcgt tggcgagacc atcgctgcgc
     1801 agcagcggcg cggcgagcga gcgatcgccg agctgcggcc gttccggccc gccgagcgat
     1861 ttcagcatgc cgtgcacggc gtggctctcg ccttcgggct cccagcccgc cgcgggactg
     1921 gcgatgccgg cgagcgtata cagcatggtg tcaaggtcgg gcgagaaccc cagaccgaga
     1981 tgctcgtagt cgtcgccggt gttgacgatc accgcgaggt ttttgccgcg cagccggtac
     2041 aggccgtcgg cgagcttcgc ggcgccgacc tgcccggcga gtgcgaccac gcttcccatg
     2101 cggcgattct agtcgctaga acttctctgc ccaagggcga acatcggcct cgaaggtcca
     2161 ggccgagcga ggctgggcgt gcagctgcca gaacagctcc gcgatggcgt cggtggcaag
     2221 catgccgtcg ggcggcagat cctttacccg ctccgccatg cggctgcgcg tgcgggggtt
     2281 gtcgatcatg ccgtcgatca cgacgtgcgc cacgtggatg ttcttcggcc cgagctcgcg
     2341 cgccatcgat tgcgccacct gccgcaggcc gccttttgcc gccgcgaagg cagcgaactg
     2401 cgccgcgccg cgcagcgatg cggtggcgcc ggtgaagagg atcgagccct tgccgcgcgg
     2461 cgccatgcgg cgcgcggctt cccggccaaa gaggaatccc gcgtagcagc ccagccgcca
     2521 gactttctcg aacatctccg ccggcgtgtc gaggatcgag gcacggaaat tggcgccggc
     2581 gttgaacacc gcgacctcga ccggcgcgcg tttctcgacc cggtcgaaca gcgcaacgac
     2641 ctcggcctcc tcgcgcaggt ccactttccc cgggccgtcc ctgcttctgg acgccaggac
     2701 gacctcgtaa ccgccttgcg tgaaccgtct gccgagcgcc gcgcccagcc cttcgcccgc
     2761 tcccccgatc aatgccacgc ccgtcgccga catgcggcga ttctatcttc gcagcacgca
     2821 cacggtgccg agaacgacga gcgcggcgcc cgccagcatc gagagcgaaa tcgtttcgcc
     2881 gaggaacagg gctccccagc acacgccgaa aatcgggatc agataggtga cggtgagcgc
     2941 gcgcgtcggc ccgatgtcag tgaccaggcg aaagtagagc aggtaggcga cggcgccgca
     3001 gaccagccca agcgcgagca tgctggcagc aaccagcggc gtgggcgccg cagccggcgg
     3061 ccaaagcgcg atgaatggaa tgagcaggac gcctgccgcc aattgcgtgc cgacggccat
     3121 gccgcgcgag ggcaccccgc tcgcccagcg cttcatgtaa gccccggcga ggccgtagca
     3181 aaacgcggcg gccagcgcgg cgatgatcgc cggatagacg agagtcgccc ccggctccgg
     3241 gcgggtgacg agcgcgactc ccaccactcc gatgaccagc ccgagcacgc ggcgcgcgtt
     3301 caggcgctcg cccagcagca gggccgacca gagcgcgccc cacatcggag aagtggcatt
     3361 gagcaccgac atcagcccgg ccgtgaggct gagagcggcg taagcccaca gcaggaatgg
     3421 caggcccgaa gtcagcatgc cgacgatcac gtactggcgg ccccagcggc gccattcggg
     3481 gttgaacccc gtggctgcga accagatcgc gagagcgatg ccggcgatga gcgtgcgcag
     3541 ttccgccgtc agcaccgggc cgagcagcgg ggccgtaatg cggttgaaca ggaacgatgc
     3601 gccccagatc gcggcgagca ggacgagccg ggtactgtct cggagcgtca tcgaggacta
     3661 gaattctctc aaggagagga gaacgccatg gaaaacatgc tcccagttgc gggcaacgga
     3721 ctgctggacc gccgcgcttt tcttcgcggc ggcgccgcgc tcgccgctgt catgaccggc
     3781 tacaccgtcg cccgacccgc ggcagcggcg ccgctcgcgg atgagccctg gagcctcgcg
     3841 cccggggcga tctcgcccgc ttatgaaaag cgctcgaggt tcgaggagaa agtggcgcgc
     3901 acgctgagca atccgaaggg cgagacgcgc acctcgcacg cccgcactcc gcaccacctg
     3961 atcaacggca gcttcacgcc gaacggcctg catttcacca tcgtgcactc gggcacgccg
     4021 gacatcgacc ccgaccggca ccgcctcgtg atccacggcc tggtgaagcg gccgctcgcc
     4081 ttcagcctcg acgcgctcgg gcgctacccg atggtctcgc gcatgacgtt cgtcgaatgc
     4141 ggcggcaaca gcgcgccgct gttctcgccc gagccggtgc aggcgagcgt gcaggcgctg
     4201 cacggcctcg cctcctgcgc cgagtggacc ggcgtgatgc tctcctcgct gctggaggaa
     4261 tgcggcgtcg agccgaaagc gagatggctg atcgccgaag gcgcggactc gctcgccctg
     4321 agccgcagcg tgccgctcgg caaggctttg gacgacgcga tggtggcgct ctaccagaac
     4381 ggcgagcgcc tgatgccggg aaacggctat ccgatgcgcc tgctgctgcc gggctgggaa
     4441 ggcaacatga acgtcaaata cctgcggcgc atcaagctgg tcgagcagcc ggcgatgagc
     4501 tactacgagg cgcgcactta ctcgcagatc ctgccgggcg gcaaggcgta caagttctac
     4561 ttcgtgcagg aagtgaaatc gttcgtcacc catccctctc cgggactgaa gctgaacggc
     4621 cccggcttct atgaggtctc cggcatcgcc tattccggca acggcaggat ttcgaaggtg
     4681 atggtttcgg ccgacggtgg ccgcagctgg gcgcaggcgg cgctgcagga gccggtgctc
     4741 tccaaggcct tcacccgctt ccgcgtgccc tggcgctggg acggcggtcc ggcggtattg
     4801 caaagccgcg cctgggacga ggccggctat gcgcagccga cgcgcgcgca gttcgtggcc
     4861 gtgcgcggcg aggcgtcgag cgtcccgccc gtcacggcgt tcccgaacca gcacttcaac
     4921 agcatcacca gctgggcagt cgat
//
LOCUS       DASPXW010000085         4605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1414884, whole genome shotgun sequence.
ACCESSION   DASPXW010000085 DASPXW010000000
VERSION     DASPXW010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4605
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414884"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VEL04_02015"
     CDS             <1..441
                     /locus_tag="VEL04_02015"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HYT47173.1"
                     /translation="LDAVIYRGRTRFGGNVFPRQAGLRPALRAIKSGAIYYYLPDMDL
                     RPKRQLFVPFFGVPAATVTGLSYIARLTGAAIVPCVTRMLPGGRGYVVRLYPAWNDFP
                     SGDDAADARRMVAFIEERVREMPEQYLWMHKRFKSRPQGEAEFY"
     gene            438..1331
                     /locus_tag="VEL04_02020"
     CDS             438..1331
                     /locus_tag="VEL04_02020"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYT47174.1"
                     /translation="MKVAVIGAGHMGRYHAEKFARLPGVELAAVVDPDPAKAGISDYR
                     TILAKVEAAVVAVPTDRHHEVARTCLESGLHVLVEKPLAATLEQADELVDLAFKKKLT
                     LQVGHVQRYSTAFQALARRVQKPQFIDAERLAGFKQRGAEVDVILDLMIHDLDLALSL
                     ARAEVSGVSACGFPVLTEDIDIASARVDFANGCVADLSASRVSQAVVRKFRVFQAGLY
                     ASADLQAGKLRYVKQADGVIQENEETHAGGDALEAQAQAFVDAVRERKPPAVDGTEGR
                     RVLKLALDIGRLVRERLRRFE"
     gene            1328..2428
                     /locus_tag="VEL04_02025"
     CDS             1328..2428
                     /locus_tag="VEL04_02025"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HYT47175.1"
                     /translation="MKSPIAMVDTAAEYRALKSEIDAAVGRVLASGRYVLGPEGEALE
                     CELAAFTGAPHAVGVNSGTDALHFALVAVGIGPGDEVVVPSFTFFATAEAVSYTGATP
                     VFADIDSPTFNLSPASFEKTITAKTRAVIAVHLFGQSAALDEISRICTQRDLILVEDC
                     AQALGADYKGRRVGSWGDFGCFSFYPTKNLAAVGDAGLVTMREKRHADTIRMLRHHGS
                     RQTYLHERVGWNSRLDELQAAVLRVKLRKLDEFNAARRRVAQRYRERLAGAALELPAE
                     DARGTHVYHQFTIRAERRDAIREALARQGIASSIFYPLPLHRQPAYAAANAGLALPVS
                     EAVAKTVLSLPIHPLLDDAAVDRVCQAVRSCS"
     gene            2442..3242
                     /gene="dapF"
                     /locus_tag="VEL04_02030"
     CDS             2442..3242
                     /gene="dapF"
                     /locus_tag="VEL04_02030"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HYT47176.1"
                     /translation="MKLPFTKMQGAGNDFVVLDFTARRFDLTASQIRRIADRHLGVGC
                     DQVLVLERPAGSGADFRYRIFNADGGEVEQCGNGARCFVKFAHAKGLTTKREIRVETL
                     GGTIRPRLEPDGEVSVDMGAPGFSESFIEKLELNGETVELAVLSMGNPHAVQLVADAE
                     KAPVTTQGPRIERHARFPDRVNAGYMQVLGRHRIALRVWERGAGETLSCGTAACAAAV
                     SGIMRKMIDSPVRVETRGGALTISWAGGDNAVWMKGPAETVFEGTIEA"
     gene            3250..3894
                     /locus_tag="VEL04_02035"
     CDS             3250..3894
                     /locus_tag="VEL04_02035"
                     /inference="COORDINATES: protein motif:HMM:NF016243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HYT47177.1"
                     /translation="MNAEDVFLFLRGHPQFFDQHPELLEKIYVPHPHGGRAIPLAERQ
                     ILALRDKVKLLEGKLGELIRFGEENDAISEKVHRLSVALVGARDFPALAHSLYFHLRE
                     DFAVPHVALRIWGKSVPADFDEAQAVGEAQRSHAATMGAPQCGPAAGNAFVPWFREAA
                     EHVRSVALVPLGQTAVFGLLALGSEDAQRFFPEMGTLYLRRIGELCAAGVTARL"
     gene            3948..>4605
                     /locus_tag="VEL04_02040"
     CDS             3948..>4605
                     /locus_tag="VEL04_02040"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HYT47178.1"
                     /translation="MRNYAHALDLLFFLLDQRNLDSLDAAQVRRFIGVLHAKGLSART
                     LALTLSAWRGCYRWLVRQRGFRSNPVLGIRAPKAARPLPKALSVEAAQRLLEDGEEDS
                     VVSLRDRAMFELLYSSGLRLGELVALNVDDGRLDLSQGEVTVTGKGAKTRTVPVGARA
                     RDALRQWLLVRNNLATQSEKAIFVGSRGKRIAPAVVGVRLRAWARRRGLNQPVHPHML
                     R"
BASE COUNT          736 a         1519 c         1597 g          753 t
ORIGIN      
        1 ctcgacgccg tcatctaccg cgggcgcacg cgcttcggcg gcaacgtgtt tccgcgccag
       61 gcgggcctgc ggccggcgct gcgcgcgatc aagtcgggcg ccatctacta ctacctgccc
      121 gacatggatc tgcgcccgaa gcgccagctt ttcgtgccgt ttttcggcgt gcccgccgcc
      181 acggtgaccg gactgtcgta tattgcgcgc ttgaccggcg ccgccatcgt gccgtgcgtg
      241 acgcgcatgc ttccgggggg ccgcggctac gtggtgcggc tctacccggc ctggaacgac
      301 ttcccgagcg gcgacgacgc ggcggatgcg cgccgcatgg tggccttcat cgaggagcgc
      361 gtgcgcgaga tgcccgagca gtacctctgg atgcacaagc ggttcaagtc caggccccaa
      421 ggggaagcgg aattctattg aaggtcgcag tcatcggcgc cggccacatg gggcgctacc
      481 acgccgagaa gtttgcgcgc ctgccgggcg tcgagctcgc ggcggtggtg gatcccgatc
      541 cggcgaaggc cgggatctcc gattaccgga cgatcctggc caaggtcgaa gccgcggtgg
      601 tcgcggtgcc gaccgaccgc caccacgaag tggcgcgcac ctgcctggaa agcgggctcc
      661 acgtcctggt ggagaagccg ctcgccgcga cgctcgagca ggccgacgag ctcgtggatt
      721 tggctttcaa gaagaaactt actcttcagg ttgggcacgt acagcgctat agcaccgcct
      781 ttcaagcgct cgcccggcgc gtgcaaaagc cccagttcat cgacgccgag cgcctcgccg
      841 gcttcaagca gcgcggcgcc gaagtcgacg tcatcctcga cctcatgatc catgacctcg
      901 acctcgcgct gtcgctcgcg cgcgccgagg tgagcggcgt cagcgcgtgc ggctttccgg
      961 tgctgaccga agacatcgat atcgcgagcg cgcgcgtcga cttcgccaat ggctgcgtgg
     1021 cggacctgtc ggcgagccgg gtgagccagg ccgtggtccg caagtttcgc gtctttcagg
     1081 caggtctcta cgcctcggcc gatctgcagg ccggcaagct tcgctacgtg aagcaggccg
     1141 acggcgtcat ccaggagaac gaggagacgc atgccggcgg cgatgcgctc gaggcacagg
     1201 cgcaagcctt cgtcgacgca gtgcgcgaac gcaaaccgcc tgcggtcgat ggcaccgaag
     1261 gccgtcgcgt gctcaagctc gccctcgaca tcggccgcct ggtgcgcgag cgcctgaggc
     1321 gcttcgaatg aaaagcccga tcgccatggt cgatacggcc gccgagtacc gcgcgctcaa
     1381 gagcgagatc gacgccgcag tgggccgcgt gctcgcgtcg ggccgctacg tcctcggacc
     1441 cgagggcgag gcgctggagt gcgaactggc ggcgttcacc ggcgcgccgc atgccgtcgg
     1501 cgtcaactcg ggaaccgacg cgctgcattt cgcgctggtc gcggtcggca tcggccccgg
     1561 agacgaggtg gtcgttccgt cgttcacctt tttcgccacc gccgaggcgg tgtcgtacac
     1621 cggcgcgacg ccggtatttg ccgacatcga ttcgccgacg ttcaatcttt cgcctgcctc
     1681 ctttgaaaaa accattacgg cgaaaacgcg cgcggtcatc gccgtgcatc tgttcgggca
     1741 atctgcggcg ctggacgaga tcagcaggat ttgcacgcaa agagatctca ttctggttga
     1801 ggactgtgcc caggcgctcg gcgcggatta caagggacgg cgggtgggca gctggggcga
     1861 tttcggctgc ttctcgttct acccgacgaa aaacctcgcc gcggtcggcg acgcggggct
     1921 ggtgacgatg agggagaaga gacacgccga caccatccgc atgctgcgcc accacggcag
     1981 ccgccagacc tacctgcacg agcgcgtcgg ctggaacagc cgcctggacg agctgcaggc
     2041 cgcagtgctg cgcgtgaagc tgcgcaagct cgacgagttc aacgccgcgc gccgccgcgt
     2101 cgcgcagcgc tatcgcgagc ggctcgccgg cgcggcgctc gaactgcccg cggaggacgc
     2161 gcgcggcacg cacgtctatc accagttcac tatccgggcc gagcgccgcg acgcgatccg
     2221 cgaggcgctc gcgcgccaag gcattgccag ctcgatcttc tatccgctgc cgctgcaccg
     2281 ccagccggcc tacgcggccg cgaacgcggg gctcgccctg cctgtttcgg aggcagtcgc
     2341 gaagaccgtg ctctcgctgc cgatccatcc gctgctcgac gacgccgcgg tcgatcgggt
     2401 gtgccaggca gtgcgatcgt gttcgtgaat ctgctgcgcg gatgaaactt ccgttcacga
     2461 aaatgcaggg agcgggaaat gattttgtgg tcctggactt cacggcaagg cgttttgatc
     2521 tgacggcaag tcagatacga agaatcgccg accgccatct cggggtcggc tgcgaccagg
     2581 tgctggtgct cgagcggccg gccggcagcg gcgccgattt ccgctaccgt atcttcaatg
     2641 ccgacggcgg cgaggtggag cagtgcggca acggcgcgcg ctgcttcgtg aagtttgcgc
     2701 acgccaaggg ccttaccacg aagcgcgaga tccgcgtcga gacgctcggc ggcacgatcc
     2761 ggccgcgcct cgagcccgac ggcgaggtga gcgtggatat gggtgctcca gggttttccg
     2821 aatctttcat tgagaaactg gagctgaacg gcgaaaccgt cgagctcgcc gtgctctcga
     2881 tgggcaaccc gcacgcggtg cagctggtcg ccgatgccga gaaagccccg gtgaccacgc
     2941 agggtccgcg catcgagcgc cacgcgcgct tccccgaccg cgtcaacgcg ggctacatgc
     3001 aggtgctcgg ccggcatcgc atcgcgcttc gcgtgtggga gcgcggcgca ggcgagacgc
     3061 tttcctgcgg cacggcagcc tgcgcagcgg ccgtttcggg gatcatgcgg aagatgatcg
     3121 attctccggt gcgcgtcgaa acgcgcggcg gagcgttgac catctcctgg gccggcggcg
     3181 acaatgcggt gtggatgaag ggccccgccg aaacggtgtt cgaaggcacc atcgaagctt
     3241 gaaacaacaa tgaatgcaga agacgtcttc ctgttcctcc gcggccaccc gcagttcttc
     3301 gaccagcatc ccgagctgct cgagaagatc tacgtgccgc acccgcacgg cgggcgcgcg
     3361 atcccgctcg ccgagcgcca gattctggcg ctgcgcgaca aggtgaagct gctcgagggc
     3421 aagctcggcg agctgatccg cttcggcgag gagaacgacg ccatcagcga gaaggtgcat
     3481 cgcctgtcgg tcgcgctggt cggcgcgcgc gatttcccgg cgctcgcgca ttcgctgtac
     3541 ttccatctgc gagaggactt cgcggtgccg cacgtggcgc tgcgcatctg ggggaagagc
     3601 gtgccggccg atttcgacga ggcgcaggcc gtgggcgagg cgcagcgcag ccacgccgcg
     3661 accatggggg cgccgcagtg cgggcctgcc gcgggcaatg cgttcgtgcc gtggtttcgc
     3721 gaagctgcgg agcatgtgcg ctcggtggcg ctggtgccgc tcggccagac cgcggtattc
     3781 gggctgctcg cgctcggcag cgaggacgcg cagcgctttt tccccgagat ggggaccctt
     3841 tacctgcgac gcatcggcga gctatgcgcg gccggagtga cggcgcggct ttagtcgaag
     3901 aataccttga tgcgctgaag catcagcgcc ggctttcgcc agcgacgttg cgaaactacg
     3961 cgcacgccct cgacctgctg ttctttctcc tggaccagcg aaacctggat tcgctcgacg
     4021 ccgcgcaggt gcggcgcttc atcggggtgc tgcatgcgaa agggctgtcg gcgcgcacgc
     4081 tcgcgctgac gctttcggcc tggcgcgggt gctatcgctg gctggtgcgc cagcgcggct
     4141 tccgctcgaa cccggtgctc ggcatccgcg cgccgaaggc ggcgcgcccg ctgccgaagg
     4201 cgctctcggt cgaggcggcg cagcgcctgc tcgaagacgg ggaagaagac tcggtcgtct
     4261 cgctgcgcga ccgggcgatg ttcgagctgc tgtactcgtc gggattgcgc ctcggcgagc
     4321 tcgtcgccct caacgtcgac gatggcaggc tcgacctctc gcagggcgag gtgacggtga
     4381 ccggcaaggg agcgaagacg cgtaccgtgc cggtcggcgc cagggcgcgc gacgcgctca
     4441 ggcagtggct gttggtcaga aataatttgg caactcaaag tgaaaaagca atcttcgtgg
     4501 gaagccgcgg caagagaatc gcgcccgccg tggtcggcgt gcgcctgcgg gcctgggcgc
     4561 ggcgccgcgg cctgaaccag ccggttcatc cgcacatgct gcgcc
//
LOCUS       DASPXW010000086         6400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1289767, whole genome shotgun sequence.
ACCESSION   DASPXW010000086 DASPXW010000000
VERSION     DASPXW010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6400
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289767"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..975)
                     /locus_tag="VEL04_02045"
     CDS             complement(103..975)
                     /locus_tag="VEL04_02045"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYT47179.1"
                     /translation="MVPQAPGSATDNIARLIAPELARQLGQPAVVDNRPGGALTIGID
                     AVAKAPPDGYTIGMGPVGALAITRHMVARLPYDIERDLQPVALTTTGYMLLAASPKLP
                     VRSVEELIDYAKKNPGKLSNASSSNGSPGHVSGELFKSMTGTDIVHIPYKGGAAAIAD
                     LIAGNVQLMFESTNSIAPHVVAGRVRALAVTGLKRATSLPGVPTLDEAGVPGYEVTAW
                     TGIIAPAGLPRPVLDRLNAAVNAAISEPQAKQRLAQLGSEGGGGTPEDYAALIRRDSA
                     KWAEVIRRSGARID"
     gene            complement(1056..1502)
                     /locus_tag="VEL04_02050"
     CDS             complement(1056..1502)
                     /locus_tag="VEL04_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HYT47180.1"
                     /translation="MKTRIAKRLAIFAAVFALAACAGMRDKMPDWMPGSGATSVKLSG
                     AEEVPAASTSGSGKGSFRIAGDGSISGSVSTKGVQGTAAHIHMGAKGQNGPVILPLSK
                     DGDTYSVPEGKKLTEAQMKAFKSGSLYVNVHSNAHKGGEVRAQLQP"
     gene            1571..2764
                     /locus_tag="VEL04_02055"
     CDS             1571..2764
                     /locus_tag="VEL04_02055"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003808961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HYT47181.1"
                     /translation="MPAIAASAALKRFRILDLSRVRAGPTCVKQFADFGADVIKIESP
                     PGVDPNEAMGGPRHGPDMQNLHRNKRAMTLNLKLPEAREVLVRLVKTADVLVENYRPD
                     VKFRLGIDYESLKKINQRIVLASISGFGQDGPYRDRPGFDQIAQGMSGLMSVTGLPGQ
                     GPVRTGAAIADMAAGLFAALGIMTALLERESSGEGQWVQSSLLQSGIALLDFQAARYL
                     MKGEVPPQVGNDHPTSMPTSAYKTADGYINVGAAGEGMWKRLCNRIGREDLLAMDEFK
                     TNEGRAKHRARLNAILNESFSTRASEEWVEELNRAGVPCGPIYAMDRVFSDPQVEHLG
                     AAAEVEHPKLGRFKVLNQAVKLSRTPAAVKTATPEPGQHTDEILMELGYQKPEITRLR
                     ARGVV"
     gene            2770..3558
                     /locus_tag="VEL04_02060"
     CDS             2770..3558
                     /locus_tag="VEL04_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HYT47182.1"
                     /translation="MAGLTERIITRKAGAAGWLIFNNPERRNAVSVDMWEAIPSVLAK
                     FESDAEVRVVVLAGAGDKAFVSGADISQFESQRSSAEAVQHYEEIAETAQVRLQSFDK
                     PLLAMIRGYCLGGGLNIANICDLRIAAEDARFGIPAAKMGLGYRASSMKNLVEVVGAA
                     FAREIMITGRQFSAAEALHMGLVHKVVPVAELEKATREYCDMIAANAPLTMRAAKRII
                     REVSKKDYDVALCKSWVKECFESEDYAEGRRAFMEKRKPVFKGK"
     gene            3582..4529
                     /locus_tag="VEL04_02065"
     CDS             3582..4529
                     /locus_tag="VEL04_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYT47183.1"
                     /translation="MVFFIAALLASGAAAAQAFPSRTVTIVVPYPPGGLIDIVARLIQ
                     PRMQAELGQPVVVENRSGAGGNVGAEWVARATPDGHTLLLANPSLGISPSMYPKLNYS
                     PLKDFAYVGLYGTVPNVLVVHPSLPANTVQELIAYLKKNPGKLNYASPGYGTSPQMSM
                     ELFKGMTGTFIVHIPFRGSGPAQAAMLAGETQLMFDNLPPQMPHIKSGKVRPLAVTSL
                     KRSAAVPEVPTLDEAGLKGYEVTAWFGLAAPAATPREAVMRLNQALNRTTQDAQVRDT
                     LESRGATVIQGTPEACLAFVRNEVEKWGPVVKRAGVVPE"
     gene            4540..5496
                     /locus_tag="VEL04_02070"
     CDS             4540..5496
                     /locus_tag="VEL04_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYT47184.1"
                     /translation="MRCFAAVFALAFAAGAPAQGGYPSKPVTTVVGFEPGGGTDTVAR
                     IVAKTLGENLGQQMVVENRAGAGGNIAVDNVAKSAPDGYTLVLANVGALAVNPHILKT
                     PYDPLRDLTPITMAVVFANVLVVQPALAVNSLADYVKLAREKPDTITYASSGIGGAGH
                     LAGELLKRVAHIDIVHVPYKGGGPAMQGFLGAQVDSFFATPVSSISQIKAGKARPIAT
                     TGSRRAALLPNVPTVAESGYPGYEALNWYAYLGPARMPKELVDRLNRELVKVLGTREV
                     IALLDKQGVEAQPGTPEELARYIEREYRTWGKVVKEAGIKAQ"
     gene            5724..>6400
                     /locus_tag="VEL04_02075"
     CDS             5724..>6400
                     /locus_tag="VEL04_02075"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYT47185.1"
                     /translation="MRELGYVEGKNLVIEWRFADGNYDRFPALASELAMLKVDVLVTH
                     VTATMRALQRATSTIPIVVTSLTDPVGGGYAASLARPGGNITGLSNVTIDATSKRFEL
                     LTAMLPKLSRLAVLLNPGNPSRAVMLQSVKDAAHRTRIKIVPVDARTPQEIERAFAAM
                     AQQRVQALIVANDGFFLGQARTLAALTLKNRLPAMFPYREIVTMGGLMSYGQNYVDTY
                     RQAAMYV"
BASE COUNT         1113 a         2074 c         2175 g         1038 t
ORIGIN      
        1 gcgcccgcct gcgaataggt ggcgatgccg ctggggtcgt cgtcggccgc cccggtcacc
       61 agccccggcc cgagccgctt gagcagcggc ttgcggcgcg cttcagtcaa tcctggcgcc
      121 ggagcggcgg atcacctcgg cccatttcgc cgaatcgcgc cggatgagcg cggcgtaatc
      181 ctccggcgtg ccgccgccgc cttcgctgcc gagctgcgcc aggcgctgct tcgcctgcgg
      241 ctcggaaatc gcggcattga ccgcggcatt cagcctgtcg agcaccggcc ggggcaagcc
      301 tgcgggggcg attatcccgg tccaggcggt aacctcgtag cccggcacgc ccgcctcgtc
      361 gagcgtcggc accccgggca ggctggtggc acgcttcagc ccggtgaccg cgagcgcgcg
      421 cacgcgtccg gcgacgacat gcggcgcaat cgagttggtg ctctcgaaca tgagctgcac
      481 gttgcccgcg atgaggtcgg cgatcgcggc cgcgccgccc ttgtagggaa tgtgcacgat
      541 atccgttcct gtcatcgact tgaacagctc gccgctcacg tgccccgggg agccgttgct
      601 cgacgacgcg ttggaaagct tgccgggatt ctttttcgca tagtcgatca attcctcgac
      661 gctgcgtacc gggagcttcg gtgaagcggc aagcagcata tagccggtgg tggtcagcgc
      721 caccggctgc aggtcgcgct cgatgtcgta gggcagcctc gcgaccatgt ggcgtgtaat
      781 cgccaatgct cccaccggtc ccatcccgat ggtgtagccg tcgggcggcg ccttggcgac
      841 ggcatcgatg ccgatggtga gcgcgccgcc cggacggttg tcgaccacgg cgggctgacc
      901 gagctgcctg gcgagctcag gcgcgatcag gcgcgcgatg ttgtcggtgg cgctgcccgg
      961 cgcctgcggc accacgaagc ggatcggccg ctcgggatat tgagcggccg ccgcgtgcgc
     1021 ccagagggcg cacgctacgg caatgcagaa aaacttcagg gctgcagctg tgcgcggact
     1081 tcgccgccct tgtgggcgtt gctatgcacg ttcacgtaca ggctgcccga tttgaacgct
     1141 ttcatctgcg cctcggtcag tttcttgcct tcgggcacgc tgtaggtgtc gccgtccttg
     1201 ctgagcggca ggatcaccgg gccgttctgc cctttggcgc ccatgtggat gtgcgccgcc
     1261 gtgccctgta cgcccttagt gctgacgctg ccgctgatcg agccgtcgcc ggcgatgcgg
     1321 aagctgcctt tgcccgagcc cgaggtgctc gcggccggca cttcttccgc gccgctcagc
     1381 ttcacgctgg ttgcgccgct acccggcatc cagtcgggca ttttgtctct catcccggcg
     1441 caagctgcga gcgcgaagac ggctgcgaag atcgccaaac gcttggcaat gcgggtcttc
     1501 atggtcattc tcctcctcat gatatgaacc aaaccgtaac cgaaacaccc ggacggctag
     1561 aatattcctc atgccggcca tcgctgcctc ggcagcgctg aagcgctttc gcattctaga
     1621 cctgtcgcga gtgcgggcgg gaccgacctg cgtgaagcag ttcgccgact tcggcgccga
     1681 tgtcatcaag atcgagtcgc cgcccggggt cgacccgaac gaggccatgg gcgggccgcg
     1741 ccacggcccg gacatgcaga acctgcaccg caacaagcgc gcgatgacgc tcaacctcaa
     1801 gctgccggag gcgcgcgaag tgctggtgcg gctggtgaag accgcggacg tgctggtcga
     1861 gaactaccgc cccgacgtca agttccgcct cggcatcgac tacgagtcgc tgaagaaaat
     1921 aaaccagcgc atcgtcctgg cgagcatttc gggcttcggc caggacggcc cgtaccgcga
     1981 ccgcccggga ttcgaccaga tcgcgcaggg catgagcggg ctcatgtcgg tgaccgggct
     2041 gccagggcaa ggcccggtgc gcaccggcgc ggcgatcgcc gacatggcgg ccggcctgtt
     2101 tgccgcgctc ggcatcatga cggcgctgct cgagcgcgag tcttcgggcg agggccagtg
     2161 ggtgcagagc tcgctgctgc agtcgggcat cgcgctgctc gacttccagg cagcgcgcta
     2221 cctgatgaag ggcgaggtgc cgccgcaggt gggaaacgac caccccacca gcatgccgac
     2281 ctccgcgtac aagactgccg acggctacat caacgtcggg gccgcaggcg agggcatgtg
     2341 gaagcggctg tgcaatcgca tcggccgcga ggatctgctg gcgatggacg agttcaagac
     2401 taacgaaggc cgggcgaagc accgcgcccg gttgaacgcc atcctgaacg agagcttctc
     2461 gacccgcgcc agcgaggaat gggtcgaaga gctcaatcgg gccggagtgc cttgcggccc
     2521 gatctacgcc atggaccgcg tgttctccga tccgcaggtc gagcacttgg gcgccgcggc
     2581 cgaggtcgag catccgaagc tcggccgctt caaggtcctg aaccaggcgg tgaagctctc
     2641 gcgcacgccg gcagcggtga agaccgccac gcccgagccc ggccagcaca ccgacgagat
     2701 cctgatggaa ctgggttatc aaaaaccgga gatcacaagg ctgcgggctc ggggggtggt
     2761 ttgactatcg tggcaggact gaccgaacgc atcatcacgc gcaaggcggg cgcggcggga
     2821 tggctgatct tcaacaatcc ggagcggcgc aacgccgttt cggtcgacat gtgggaggcc
     2881 attccttcag ttcttgcaaa atttgaatcg gatgcagagg tgcgagtggt ggtgctcgcc
     2941 ggggcgggcg acaaggcgtt cgtctcgggc gccgacattt cgcagttcga aagccagcgc
     3001 tccagcgccg aggcggtgca gcattacgag gagatcgccg agaccgcgca ggtgcgcttg
     3061 cagagcttcg acaagccgct gctggcgatg atccgcggct actgcctcgg cggcgggctg
     3121 aacatcgcca acatttgcga cctgcgcatc gctgcggagg acgcgcgctt cggcatcccg
     3181 gcggcgaaga tggggctcgg ctaccgcgcg agctcgatga agaacctggt ggaggtggtc
     3241 ggcgccgcgt tcgcgcgcga gatcatgatc accggccgcc agttcagcgc cgccgaagcg
     3301 ctgcacatgg gcctggtgca caaggtagtg ccggtcgccg agctcgagaa ggcgacgcgc
     3361 gaatactgcg acatgatcgc ggccaacgcg ccgctcacca tgcgcgcagc gaagcgcatc
     3421 atccgcgagg tgtcgaagaa ggattacgac gtcgcgctgt gcaagtcgtg ggtgaaggaa
     3481 tgcttcgaga gcgaggatta tgccgaaggc cggcgcgcct tcatggaaaa gagaaagccc
     3541 gtattcaagg gtaagtagca cgaggaggaa atatgggaag gatggtcttt ttcatcgcgg
     3601 ccctgctcgc gagcggcgcg gcggcggcgc aggcatttcc gagccgcacg gtcacgatcg
     3661 tggtgccgta tccgcccggc gggctgatcg acatcgtggc gcgcctcatc cagccccgca
     3721 tgcaggccga gctcggccag ccggtggtgg tcgagaaccg ctcgggggcc ggcggcaacg
     3781 tcggcgccga atgggtggcg cgcgcgacgc ccgacggcca tacgctgttg ctcgccaacc
     3841 cttcgctcgg catcagcccg agcatgtatc ccaagctgaa ttattcaccg ctcaaggact
     3901 tcgcctacgt cgggctgtac ggcacggtgc cgaacgtgct cgtggtgcac ccatcgctgc
     3961 cggcgaatac agtgcaggag ctgatcgcct acctgaagaa gaatccgggc aagctcaact
     4021 acgcctcgcc cggctatggc acctcgccgc agatgtcgat ggagctgttc aagggcatga
     4081 ccggcacctt catcgtgcac atccccttcc gcggcagcgg gcccgcgcag gcggcgatgc
     4141 tcgccggcga gacgcagctc atgttcgaca acctgccgcc gcagatgccg cacatcaagt
     4201 cgggcaaggt gcggccgctc gccgtgacca gcctcaagcg ctcggcggcg gtgcccgagg
     4261 tgccgacgct ggacgaggcg ggactcaagg gctacgaggt cactgcctgg tttggccttg
     4321 cagcgccggc ggcgacgccg cgcgaggcgg tgatgcggct caaccaggcg ctcaaccgca
     4381 cgacgcagga cgcgcaggtg cgcgatacgc tcgagtcgcg cggcgcgacc gtgatccagg
     4441 gcacgcccga ggcctgcctc gcgttcgtga ggaacgaagt cgagaagtgg gggccggtag
     4501 tgaaacgcgc cggcgtcgtc ccggagtagg aacacggcca tgagatgctt cgcggcagtt
     4561 tttgcattgg ccttcgcagc gggcgccccg gcgcagggcg gttatcccag caagccggtc
     4621 accacagtcg tgggcttcga gcccggcggc ggcaccgata ccgtggcgcg catcgtcgcc
     4681 aagacactcg gcgagaacct cgggcagcag atggtggtcg agaaccgcgc cggcgcgggc
     4741 ggcaacatcg cggtcgacaa cgtcgcgaag agcgcgcccg acggctacac cctcgtgttg
     4801 gcgaacgtgg gcgcgctcgc ggtgaatccg cacatcctga agacgcctta cgacccgctg
     4861 cgcgatctca cgccgatcac catggcggtg gtgttcgcca acgtgctggt ggtgcagccg
     4921 gcgctcgcgg tgaattcgct cgccgactac gtcaagctgg cgcgcgaaaa gccggacacg
     4981 attacctacg cgtcctccgg gatcggcggc gccgggcatc tggcgggcga gctgctgaag
     5041 cgcgtcgcgc acatcgacat cgtgcacgtg ccgtacaagg gcggcgggcc ggcgatgcag
     5101 ggctttctcg gtgcccaggt ggattcgttc ttcgccacgc cggtgtcgtc gatctcgcag
     5161 atcaaggccg gcaaggcgcg gccgatagcc acgacgggtt cgaggcgcgc tgcgctgctg
     5221 ccgaacgtgc cgacggtcgc cgaatccggc tatcccggct atgaggcgct caactggtac
     5281 gcctacctcg gtccggcgcg catgccgaag gagctcgtcg atcgcctgaa ccgcgagctg
     5341 gtgaaagtcc tgggcactcg cgaggtgatc gcgctgctcg acaagcaggg cgtcgaggcg
     5401 cagcccggca cccccgagga gctcgcgcgc tacatcgagc gcgagtaccg gacctggggc
     5461 aaggtggtga aagaggcggg catcaaggcg cagtaaaacc gaggacagac cacgctaatc
     5521 ggaaaaatcg gggtcagacc ccgaataggg gagggtggat ggaaagacgc agaaagctgc
     5581 tgatcgcgct cagcgcaggc gcgtttctcc cctgcgcgtc tttcgccgag caagggaaag
     5641 tctggcgcat cggctttctc gccgcgcgtt cgcgttcgac accgtcgaag cccgatatct
     5701 attacgacgc cttcgtggag acgatgcgtg agctcggcta cgtcgaggga aagaatctgg
     5761 ttatcgagtg gcgctttgcc gacgggaatt acgatcgctt tcccgcattg gcgtccgagc
     5821 tggccatgct gaaagtcgac gtgctcgtga cgcatgtgac cgcgacgatg cgggcactgc
     5881 aacgcgcgac gagcacaatt ccgatcgtcg tcacatcact caccgacccc gtgggcggcg
     5941 gatatgccgc cagcctggcc aggcccggag gcaacatcac cgggctctcg aacgtcacga
     6001 tcgacgcgac ctcaaaacgc ttcgagctgc tgacggcgat gctgcccaag ttgtcgcgcc
     6061 tcgctgtcct gctcaatccg gggaacccgt cgcgcgccgt catgttgcaa agcgttaagg
     6121 acgccgcgca ccgcacccgg atcaaaatcg tgcccgtgga tgcaaggact ccgcaggaaa
     6181 tcgagcgcgc cttcgccgcg atggcgcagc aacgggtgca ggcgctcatc gtcgcgaatg
     6241 acgggttctt cctcggccaa gcacgcacgc tcgcggcgct gacccttaag aacaggctac
     6301 cggcgatgtt tccttatcgc gagattgtca cgatgggcgg cctgatgagc tatggccaga
     6361 actacgtcga cacctaccgc caggctgcaa tgtacgtgga
//
LOCUS       DASPXW010000087         4526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1539339, whole genome shotgun sequence.
ACCESSION   DASPXW010000087 DASPXW010000000
VERSION     DASPXW010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1539339"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..1428
                     /locus_tag="VEL04_02080"
     CDS             223..1428
                     /locus_tag="VEL04_02080"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYT47186.1"
                     /translation="MKRREILKIGAAAASLGFPAIIRAQPKEIVMLGLWDQTGAFADV
                     GPINDRGMKMALEERNMQILGRPIKYITRDGGTQAGTSTRRTEEAVDAEGAKFVIGPW
                     SSGVALAVSEVAKRKKVLHFFSGGTEDISGARCHRYSFQWAASPYTAAKTVVDNFMKA
                     NPKAKRWHLLVADYAFGWSVEKYVKEVGKQHGLEFVGADRHPLGEREFSNYVQKAAAN
                     KPDVVAMINFGLDAVSGAREIFNFGLTPKIPLIMTWSSGVEELVQLTPEIRENIWVGS
                     NFYYTADTPVAKAFVKSYNAKFGNPPGYAPSAAYAMTRLMLAGFDKAKSAEVPDVIRA
                     LEGIEVTDLVGRMRVDPKTHQSLRPYFFMRCKPKSAMKHPTDFADIIATGDAPLPAEY
                     AACKDIGSF"
     gene            1432..2289
                     /locus_tag="VEL04_02085"
     CDS             1432..2289
                     /locus_tag="VEL04_02085"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYT47187.1"
                     /translation="MNLLFSQILNGIATGMLYALMGLGLSLITGVLNIPNFAHGALFA
                     IGAYLLVTTVNVIGNFWLALVVAPVGVALLGLAIEYAGIRRLYAAGHDYQLLFTFGLA
                     LILSEGIILVWGPVGYSYLPPPALRGGLDLGFTMYPKYRIFVMVAAGLLVLATWLFLE
                     KTRYGAIMRAGIENKEMVSLLGIDIHRLFTVAFALGAYLAGIAGALTAPIRGINPGMG
                     VDMLGIAFVVVALGGLGNLLGAIAAGLLIGVAQAIVAAYWTEASTAVIFAVMAAVLLV
                     RPQGLFGIR"
     gene            2286..3212
                     /locus_tag="VEL04_02090"
     CDS             2286..3212
                     /locus_tag="VEL04_02090"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYT47188.1"
                     /translation="MSRASIAVLVSIILLPLVVRHAIATEIWIFAIFGLGLNLLLGYT
                     GLLSFGQSTFFGSAAYVAGWLLKHYAIDVFLALGVGVAVGAVSALLVGYLCVQRSGLY
                     FIMLTFALNQLFYFIAYQWTSVTGGEDGMAGIPRPALLGIDFKDPLNYYLFVSLLFLI
                     SLWLMQRIVESPLGRILQAIRENEVRAEAVGYNVPRFKLLAFVIGGAFSGLAGVLYAM
                     LFGIMPLEAISFVFSGNVVFATLIGGSGSLYGPIIGSFVFIWLSESVSVLWARWPLLL
                     GLAFVIVVLFLRGGVVEAWSRFWLWHSSKQRT"
     gene            3185..3934
                     /locus_tag="VEL04_02095"
     CDS             3185..3934
                     /locus_tag="VEL04_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT47189.1"
                     /translation="MAFLETKNLTKAFGALAAVNDVSLSIEAGTLHSIIGPNGAGKTT
                     LFNLLTGSFPPTSGKIVFDQKEITGTPAHRIAHLGLARSYQRTSVFPAFSLLDNVWTA
                     AFATGRSWNGLFFKNTNQYPEAAERARQALSDVGLSDKSSQLAREISHGEQRQLELAI
                     ALAAAPRVLLLDEPAAGLSPEETRRMVALVRTLKGRYTIVLIEHNIDIIMSVSDRISV
                     MHFGAIIAEGTPGEIQRNAEVRRAYLGGVAA"
     gene            3931..>4526
                     /locus_tag="VEL04_02100"
     CDS             3931..>4526
                     /locus_tag="VEL04_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT47190.1"
                     /translation="MILEIKAIDTYYGLGHILHGLSLGVGEGEVVAVLGRNGAGKTTT
                     LRSVTGLTPPRAGEIRYKGRDIAGLDPHRISRMGIALVPETRDIFSYLTVSENLAIAR
                     RNASRWQMDTVLERFPNLKERMNNKGRQLSGGEQQMLAIARALLTGPDLLLLDEPSQG
                     LAPLVVDAVMGTIRTLKQEHVSMLLIEQNAEMALQLADR"
BASE COUNT          721 a         1550 c         1425 g          830 t
ORIGIN      
        1 ccggcgtcga gggcaagctt cttggcgctg cgcgaccggc catccatcag cgcgagcacc
       61 atcgcggcgc ggctgggatc cgcgagcatg gcggcggtgg tggcgacacg ttggggctgc
      121 acggcgcgat tgtcccgctc cctgcgcgtg cgcgtccatt cggaacactt cgaccgccgt
      181 cgaactgttt ggggtaggct cgcctcttcc agggaggagc acatgaaacg cagagagatc
      241 ctgaagatcg gggccgcagc cgcgagcctc gggttccccg ccatcattcg cgcgcagccg
      301 aaggagatcg tgatgctcgg cctgtgggac cagaccggcg ccttcgccga cgtcgggccg
      361 atcaacgatc gcggcatgaa gatggcgctg gaagagcgca acatgcagat cctcggccgg
      421 ccgatcaagt acatcacgcg cgacggcggc acgcaggccg gcacctccac ccggcgcacc
      481 gaggaggcgg tcgacgccga aggcgcgaag ttcgtcatcg gcccctggtc ctcgggcgtg
      541 gcgctcgcgg tttcggaagt cgccaagcgc aagaaagtgc tgcatttctt cagcggcggc
      601 accgaggaca tctccggggc gcgctgccat cgctattcct tccagtgggc ggcgagcccc
      661 tacaccgcgg cgaagaccgt ggtcgacaat ttcatgaagg cgaacccgaa ggcgaagcgc
      721 tggcacctgc tggtggcgga ctacgccttc ggctggtcgg tggagaagta cgtcaaggag
      781 gtcggcaagc agcacggcct cgagttcgtc ggcgccgacc ggcatccgct gggcgagcgc
      841 gagttctcca actacgtgca gaaggcggcg gcgaacaagc ccgacgtcgt ggcgatgatc
      901 aacttcggcc tcgacgcggt ctcgggcgcg cgcgagatct tcaacttcgg cctgacgccg
      961 aagatcccgc tcatcatgac ctggtcatcc ggcgtcgagg agctggtgca gctcacgccc
     1021 gagatccgcg agaacatctg ggtcggctcc aacttctact acaccgccga cacgccggtg
     1081 gcgaaggcct tcgtcaagag ctacaacgcc aagttcggca acccgcccgg ctacgcgccg
     1141 tcggccgcct acgcgatgac gcggctgatg ctcgccggct tcgacaaggc gaaatcggcc
     1201 gaggtgccgg acgtgatcag ggcgctcgaa ggcatcgagg tcaccgatct ggtgggccgg
     1261 atgcgggtgg atcccaagac gcaccagtcg ctgcggccgt acttcttcat gcgctgcaag
     1321 ccgaagtcgg ccatgaagca cccgaccgac ttcgccgaca tcatcgccac cggcgacgcc
     1381 ccgctgccgg ccgagtacgc cgcctgcaag gacatcggca gtttttagtt tttgaatctc
     1441 cttttcagtc agatccttaa cgggatcgcg acgggcatgc tgtatgcctt gatggggctc
     1501 ggcctgtcgc tcatcacggg ggtgctgaac atccccaact tcgcccacgg cgccctcttc
     1561 gccatcggcg cgtacctgct ggtcaccacg gtcaatgtca tcggcaactt ctggctcgcg
     1621 ctcgtggtgg cgccggtggg cgtggcgctg ctgggcctcg ccatcgaata cgcggggatc
     1681 cgccggctct acgcggcggg gcacgactac cagctgctct tcaccttcgg cctggcgctg
     1741 atcctctccg agggcatcat cctggtctgg gggccggtcg gctacagcta cctgccgccg
     1801 ccggctctgc gcggcgggct cgacctcggc ttcacgatgt atccgaagta ccgcatcttc
     1861 gtcatggtag cggccgggct gctggtgctc gccacctggc tgtttctcga gaagacgcgc
     1921 tacggcgcga tcatgcgcgc cggcatcgag aacaaggaaa tggtctcgct gctcggcatc
     1981 gacatccaca ggctgttcac cgtcgccttc gccctcggcg cctacctcgc cggcatcgcg
     2041 ggcgcgctca ccgcgccgat ccgcggcatc aaccccggca tgggcgtgga catgctcggc
     2101 atcgccttcg tggtggttgc gctcggcggc ctcggcaacc tgctcggcgc gatcgccgcg
     2161 ggcctgctga tcggcgtcgc ccaggcgatc gtggcggctt actggaccga agcttccacc
     2221 gcggtgatct ttgccgtgat ggccgccgtg cttctcgtcc gccctcaggg cttgttcggc
     2281 atccgatgag ccgtgcatcc atagcagttc tggtttcaat aattcttctt cccctcgtcg
     2341 tgcggcatgc catcgccacc gagatctgga tcttcgccat cttcggcctc gggctgaacc
     2401 tgctgctcgg ctataccggc ctgctgtcct tcggccagtc gacgttcttc ggctccgcgg
     2461 cctacgtcgc cggctggctc ctcaagcact acgcgatcga cgtcttcctc gcgctcgggg
     2521 tcggcgtcgc ggtgggcgcg gtgtcggcgc tgctggtggg ctacctctgc gtgcagcgct
     2581 cgggcctgta cttcatcatg ctcaccttcg cgctgaacca gctcttctac ttcatcgcct
     2641 accagtggac cagcgtcacg ggcggcgagg acggcatggc cggcatcccg cggccggcgc
     2701 tcctcggcat cgacttcaag gacccgctca attactacct cttcgtctcg ctgctattcc
     2761 tgatttcgct ttggctgatg cagcgcatcg tcgagtcgcc gctcggcagg atcctgcagg
     2821 cgatccgcga gaacgaagtg cgcgccgagg cggtgggcta caacgtgccg cgcttcaagc
     2881 tgctcgcctt cgtcatcggc ggcgccttct cgggcctcgc cggggtgctc tacgccatgc
     2941 tgttcggcat catgccgctg gaggcgatca gcttcgtgtt ctcggggaac gtggtgttcg
     3001 cgacgctgat cggcggcagc ggctcgctct acggcccgat catcggctcc ttcgtcttca
     3061 tctggctgtc cgagtcggtg agcgtgctgt gggcgcgctg gccgctgctc ctcgggctgg
     3121 cgttcgtaat cgtggtgctc ttccttcgag ggggagtggt ggaggcctgg tcgcgtttct
     3181 ggttatggca ttcctcgaaa caaagaacct gacgaaagcg ttcggcgccc ttgccgccgt
     3241 caacgatgtc agcctcagca tcgaggccgg cacgctgcac tcgatcatcg gccccaacgg
     3301 cgcgggcaag accacgctgt tcaacctgct caccggcagc ttcccaccta cgtccggaaa
     3361 aattgttttt gatcaaaagg aaattaccgg cacgccggcg caccgcatcg cgcacctcgg
     3421 tctcgcgcgc tcctaccaac gcacaagcgt ttttccggcc ttctcgctgc tcgacaacgt
     3481 ctggaccgcc gccttcgcca ccggccgctc atggaatggc ctttttttca agaatacaaa
     3541 tcagtacccc gaggctgcgg agcgcgcgcg gcaggcgctc tccgacgtgg gtctatccga
     3601 taagagcagc caattggccc gtgaaatctc gcatggcgag cagcgccagc tcgagctcgc
     3661 catcgcgctc gccgccgcgc cgcgcgtgct cttgctcgac gagcccgccg cgggcctctc
     3721 gcccgaggag acgcgccgga tggtggcgct cgtgcgcacg ctcaagggcc gctacaccat
     3781 cgtgctcatc gagcacaaca tcgacatcat catgagcgtc tcggaccgca tctcggtgat
     3841 gcacttcgga gcgataatcg ccgagggcac gcccggcgag atccagcgca atgccgaggt
     3901 gcggcgcgcc tacctgggtg gggtggccgc ttgattctcg aaatcaaggc tatcgacacc
     3961 tactacggcc tgggtcacat cctgcacggc ctctccctcg gtgtcggcga aggcgaagtg
     4021 gtggcagtcc tcggccgcaa cggtgccggc aagaccacta ccctgcgcag cgtcacgggc
     4081 ctcacaccgc cgcgcgcggg cgaaatccgc tacaagggcc gcgacatcgc cgggctggac
     4141 ccgcaccgca tctcccgcat gggcatcgcc ctggttcctg aaacgcgcga catattcagc
     4201 tatttgacgg tgagcgagaa tcttgcgatc gcgcggcgca acgcctcccg ctggcaaatg
     4261 gacacggtgc tcgagcgctt tccaaacttg aaagaaagaa tgaacaacaa aggacgccag
     4321 ctctcgggcg gcgagcagca gatgctcgcc atcgcccgcg ccctcctcac cggccccgat
     4381 ctcctgctcc tcgacgagcc gtcgcagggg ctcgccccgc tggtcgtcga cgcggtgatg
     4441 ggcacgatcc gcaccctcaa gcaggagcac gtgagcatgc tgctcatcga gcagaacgcc
     4501 gagatggccc tgcagctcgc cgatcg
//
LOCUS       DASPXW010000088         2411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1415423, whole genome shotgun sequence.
ACCESSION   DASPXW010000088 DASPXW010000000
VERSION     DASPXW010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1415423"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VEL04_02105"
     CDS             <1..202
                     /locus_tag="VEL04_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HYT47191.1"
                     /translation="SRSYEAMARAGRQLAAAHGGRLVDDNGNSLDEHALASIGAQLEA
                     LQQELARRGIEPGSPLALRLFS"
     gene            199..2295
                     /gene="ligA"
                     /locus_tag="VEL04_02110"
     CDS             199..2295
                     /gene="ligA"
                     /locus_tag="VEL04_02110"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HYT47192.1"
                     /translation="MSKAWRSRAAELRAEIERHNRLYYVEDSPEVSDAEYDRLFRELQ
                     ELEEKHPELRSADSPTQRVGGAPLEALSQVRHAVPMLSIRTETDTEDSGAANFDARLR
                     RELDLGADAPPLEYAAELKFDGVAISLRYQRGTLVLAATRGDGRVGEDVTQNVRTIRS
                     IPLRLSGKPPKLIEVRGEIYMKREDFDRMNERQRAAGDKTFVNPRNAAAGAVRQLDAR
                     ATARRPLSFSAHGFGRVEGWKTFSKHSELLDALEGLGLPVHAERAVVHGADGLIKFHR
                     RIGERRGELPFDIDGVVYKVNSLELQRRLGYVAREPRWAVAHKFPAQEESTEVLAIDV
                     QVGRTGTLTPVARLKPVFVGGVTVTNATLHNEDELRRKDVWVGDTVVVRRAGDVIPEV
                     VAVRAKGARKPESRFEMPLACPVCGSPVVRVSGEAATRCTGGLYCKAQRKQSLMHFAG
                     RRAMDIEGLGEKLVDQLVERELVKNPADLYRLDAATLAALERMGEKSALNLVSNIDKS
                     RHGELHRFIFALGIPGVGEEVAKILARHFGTLDALLGANWSKAAADKEAIRKQNAQRR
                     KRSEPLAEVPLEGVGPELMESIEKFLGEPHNREVIGRLTDTQSGVRLRHAGAAARPEA
                     QPKTFVLTGSLSAMSRGQAQAAIEAKGHKVAASVSKKTDFVVAGEEAGGKLEKARTLG
                     VTVLDEKQFLDLLHRL"
     gene            2301..>2411
                     /locus_tag="VEL04_02115"
     CDS             2301..>2411
                     /locus_tag="VEL04_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019719781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HYT47193.1"
                     /translation="MHAKLRKAVFPVAGFGSRFLPVTKASPKEMLPIVDKP"
BASE COUNT          438 a          785 c          845 g          343 t
ORIGIN      
        1 cagccgcagc tacgaggcga tggcgcgcgc cgggcgccag ctcgctgcgg cgcacggcgg
       61 ccgcctggtc gacgacaacg gcaactcgct cgacgagcac gcgctggcct cgatcggcgc
      121 acagctcgaa gcgctgcaac aggagcttgc caggcgcggc atcgaacccg gcagcccgct
      181 cgctttgcgc cttttttcat gagcaaggcg tggcgcagcc gcgccgccga gctgcgcgcc
      241 gagatcgagc gtcataaccg gctttactac gtcgaagaca gtcccgaggt cagcgacgcg
      301 gaatatgacc gcctgttccg cgagctccag gagctcgaag aaaagcatcc cgagctgcgc
      361 agcgcggatt ctccgaccca gcgggtgggc ggggcgccgc tggaggcgct ctcccaggtg
      421 cgccacgccg tgccgatgct ctcgatccgt accgagaccg acaccgagga cagcggcgcc
      481 gccaacttcg acgcgcgttt gcgcagagaa ctcgacctcg gcgccgatgc gccaccgctt
      541 gaatatgccg ccgagctcaa gttcgacggt gtcgccatca gcctgcgcta ccagcgcggc
      601 acgctggtgc ttgccgccac gcgcggcgac ggtcgggtgg gcgaggatgt gacgcagaac
      661 gtgcgcacca tccgcagcat cccgctcaga ctgagcggca agccgcccaa gctgatcgaa
      721 gtgcgcggcg aaatctacat gaagcgcgag gatttcgacc ggatgaacga gcgccagcgc
      781 gccgccggcg acaagacctt cgtcaatccg cgcaacgccg ccgccggcgc cgtgcggcag
      841 ctcgatgcca gggccacggc gcgccgtccg ctgtcgttct ccgcgcacgg cttcggccgg
      901 gtcgagggct ggaagacatt ctcgaagcac agcgaactgc tcgatgcgct ggaaggactc
      961 ggcctgccgg tccacgccga gcgcgcggtg gtgcacggcg ccgatggcct catcaagttc
     1021 caccgcagga tcggcgagcg gcgcggtgag ctgccgttcg acatcgacgg tgtggtctac
     1081 aaggtcaaca gcctcgagct gcagcgccgc ctggggtacg tggcgcgcga gccgcgctgg
     1141 gcagtcgcgc acaagttccc ggcgcaggag gagagcaccg aggtcctcgc catcgacgtg
     1201 caggtcgggc gcaccgggac gttgacgccg gtcgcacggc taaagccggt attcgttggc
     1261 ggggtcacgg tcaccaacgc gaccctgcac aacgaggacg agctgcgtcg caaggacgta
     1321 tgggtcggcg acaccgtcgt ggtgcgtcgc gcgggcgacg tcatccccga agtcgtcgct
     1381 gtgcgcgcca agggagcgcg caagcccgag agccgcttcg agatgccgct tgcctgcccg
     1441 gtctgcggct cgccggtggt gcgcgtctcc ggagaggcgg cgacgcgctg caccggcggg
     1501 ctttattgca aggcgcaacg caagcagagc ttgatgcatt ttgcaggcag gcgcgcgatg
     1561 gacatcgagg gcctgggaga gaagctggtc gaccagctgg tcgagcgcga gctggtgaaa
     1621 aatcccgccg acctgtaccg gctcgatgcg gcgacgctcg cggcactcga gcgcatgggg
     1681 gagaagtcgg cgctcaacct ggtttccaat atagacaaat cccgccatgg ggagcttcac
     1741 cggttcatct tcgcgctggg cattccgggc gtcggcgagg aggtggcgaa gatcctggcg
     1801 cgccatttcg gaaccctgga cgcgcttctc ggggcgaact ggtccaaggc cgcggctgac
     1861 aaggaagcca tccgcaagca aaatgcgcag cgcaggaagc gtagcgagcc gctcgccgaa
     1921 gtgcctctgg aaggggtcgg gcctgagctc atggagagca tcgaaaaatt cttgggtgag
     1981 ccgcataacc gcgaggtcat tgggcggctc accgacacgc agagcggcgt gcggctgcgg
     2041 cacgccggag ccgccgcgcg cccggaggcg cagcccaaga ccttcgtgct gaccggcagc
     2101 ctctcggcaa tgtcgcgcgg gcaggcgcag gcagcgatcg aggcgaaggg ccacaaggtc
     2161 gcggcttcgg tgtcgaagaa aaccgatttc gtggtcgcgg gcgaagaagc gggcggcaag
     2221 ctggaaaaag cgcgcacact cggggtcaca gtgctcgacg aaaagcagtt cctcgacctt
     2281 ttgcacaggc tctagagccc atgcacgcca agctcagaaa ggcggtgttc ccggtggcag
     2341 gtttcggcag ccggttcctg cccgttacca aggcgagccc gaaggaaatg ctgccgatcg
     2401 tcgacaagcc g
//
LOCUS       DASPXW010000089         4027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_783670, whole genome shotgun sequence.
ACCESSION   DASPXW010000089 DASPXW010000000
VERSION     DASPXW010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783670"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VEL04_02120"
     CDS             <1..607
                     /locus_tag="VEL04_02120"
                     /inference="COORDINATES: protein motif:HMM:NF023165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HYT47194.1"
                     /translation="QQVYDDGGAIAVQPLYPNVVYVPYYDPFVVYGAWWWPAYRPVFW
                     RPWPVQRVFISVGFFPNRFDWHRRQIVVAHRPTVVVNRPTQVRVTQPQVAPGHWRRDA
                     PPRTWNGPPSPAARMQQQQQQQFLQRVPELQRRPIVQGGPVVQRVPQSLRQPIVQSRQ
                     MMVQSAPHANWRSDARPGFQRQGPSGQAQHFGRSGGHGHRG"
     gene            654..1991
                     /locus_tag="VEL04_02125"
     CDS             654..1991
                     /locus_tag="VEL04_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HYT47195.1"
                     /translation="MPRIVRDTLVTLREVALAWGPFALIALALLVAAYFVLDPTPPRR
                     VVLATGPERSAYDEFGKRYAADLKRYGLEVVLKPSRGSFENLRLLRDAKHEVDFAFVQ
                     GGSSEAERLVDEDKSGVPLVSIGSLFYEPVWVFYRSQAARPLGQIAGLKGRRVNIGAR
                     GSGTPGLMAKLLAANHLEREDLKRSNLDETPAVMALLAGELDAAVLVSAPESQMVQML
                     LQTPGVRLFEFSQAEAYARRYPFISPVTLPRGVADVARDVPPRDLSLIAATTSLVARE
                     DTHPALVQLFVQAAARIHGRAGWIARAGEFPSATHTEFPLAKDAERFYRNGPPLLQRY
                     LPFWLANLIDRMWVALFSIVVVLLPVSRLVPPLYRFRVRSRVFRWYRQLRQIEDAVGS
                     RDPQELRAELDKLDARAARIVVPLSYADELYSLRSHIELVRARLQKENARVTR"
     gene            1988..2398
                     /locus_tag="VEL04_02130"
     CDS             1988..2398
                     /locus_tag="VEL04_02130"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYT47196.1"
                     /translation="MTKPRARLRHFAVVVRDLERAAAFYEKVFGLRRVGREDLEMGSG
                     IYLSDGVINLALLKYKGESGSGLKDAASFVGAHHFGFQVDDLEAARKRIEAAGGTFFF
                     TLGKTKDDANFEMKFKDPDGVIFDISEKGWSGGP"
     gene            complement(2395..3507)
                     /locus_tag="VEL04_02135"
     CDS             complement(2395..3507)
                     /locus_tag="VEL04_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HYT47197.1"
                     /translation="MKGKVEIVGGGFAGLTAACALARRGWRVRLHERADRLRTAGAGI
                     NVYENGLRVLEALGAYQDTIRDGARARLRETRDQHDRLLSTHPWDIRVYGVLRQRMID
                     ALAVAALRAGAEIMTDSEGVSATRSGELTLADGRRLKADLIVAADGVNSQIRDSLGLV
                     SRRRYLRDGCIRLLIPTRTVEEGTDGKTIEYWSGSRRLLYNPCSTTHLYLALSMLHED
                     DAARSLPVDKAVWKRSFPRLAPLIDRIGDDGRYDRFEYIRLKRWSSGRVAVIGDAAHA
                     IPPNIGQGAGCAMMNALSLGVFLERGHDVPSALDAWERAERPLTNHTQRISLFFGMPT
                     FWPPGLRALFYSVAGRSRWLTRQRTRTARHLPTGSA"
     gene            3534..>4027
                     /locus_tag="VEL04_02140"
     CDS             3534..>4027
                     /locus_tag="VEL04_02140"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYT47198.1"
                     /translation="MLDERIARFPRRKSKAISYREAGAGQALVLLHGIGSSSASWLFQ
                     LESLKGYRLIAWDAPGYGDSDFLNEKNPRAENYAAALHGFLERLLLKDVVLVGSSLGC
                     LMAAAYAAAHPERVRSMLLLGPAAGFAGDQTKLEERLAEIDQLGPEGMAEKRSPTLVA
                     KASPP"
BASE COUNT          629 a         1364 c         1384 g          650 t
ORIGIN      
        1 gcagcaggtc tacgacgacg gcggcgcgat cgcggtgcag cccctctatc ccaacgtggt
       61 ttacgtgccg tactacgatc ccttcgtggt gtatggcgcg tggtggtggc cggcctatcg
      121 gcccgtgttc tggaggccat ggccggtgca gcgcgtgttc atctcggtcg gtttcttccc
      181 caacaggttc gactggcatc gccggcagat cgtggtcgcg catcgcccga ccgtggtcgt
      241 gaatcgcccg acgcaagtgc gcgtgacgca gccgcaggtt gcgccaggac actggcggcg
      301 cgacgcgccg ccgcgaacct ggaacggccc gcccagcccc gcggcgcgga tgcagcagca
      361 acagcagcag cagtttctcc agcgcgtccc cgagttgcag cggcggccga tcgtccaggg
      421 cggcccggtg gtgcagcgcg ttccgcagtc gctgcgccag ccgatcgtgc agagccgtca
      481 gatgatggtg cagagcgcgc ctcatgcgaa ttggcgttcc gacgcgagac cgggattcca
      541 gagacagggc ccgagcgggc aagcacaaca cttcggccgc agcggcggcc acggccaccg
      601 cggttaaacc gcgctagact ctcctcttcc ccaaggagag cctggaaagt tcggtgccgc
      661 gaattgtccg cgacacgctg gtcacgctgc gcgaggttgc gctcgcctgg gggccgttcg
      721 cgctcatcgc gctggcgctg cttgtcgcgg cgtatttcgt tctcgacccg acgccaccgc
      781 ggcgcgtggt gctcgccacc ggcccggagc gcagcgccta cgacgagttc ggtaagcgct
      841 acgcggcgga tctcaagcgc tacggcctcg aggtggtgct caagccttcg cgcggctcct
      901 tcgagaacct gcgcctgctg cgcgatgcga agcacgaggt cgacttcgcc ttcgtccagg
      961 gaggctccag cgaggcggag cggctggtcg acgaggacaa aagcggcgtg ccgctcgtct
     1021 cgatcggcag cctgttctac gagccggtgt gggtgttcta ccgcagccag gcggcgcgcc
     1081 cgctcggcca gatcgccggg ctgaagggcc ggcgcgtgaa catcggggcg cgcggcagcg
     1141 gcacgccggg tctgatggcg aagctcctcg ccgcgaatca cctcgagcgc gaagacctca
     1201 agcgcagcaa cctcgacgag accccggcgg tgatggcgct gctcgcgggc gagctcgacg
     1261 ccgcggtgct ggtgtcggcg ccggaatcgc agatggtgca gatgctgctg cagacgccgg
     1321 gagtgcggct gttcgagttt tcccaggccg aggcgtatgc gcggcgctac ccgttcatca
     1381 gcccggtcac cctgccgcgc ggcgtcgccg acgtggcgcg cgacgtgccg ccgcgcgacc
     1441 tgtcgctcat cgccgccacc acctcgctcg tcgcgcgcga ggatacgcac ccggcgctgg
     1501 tgcagctctt cgtgcaggcg gcggcgcgca ttcacgggcg ggcggggtgg attgcccgcg
     1561 cgggcgagtt tccgagcgcg acgcacaccg agtttccgct tgccaaggac gccgagcgct
     1621 tctaccgcaa cggcccgccg ctgctgcagc gctatctgcc gttctggctc gcgaacctca
     1681 tcgaccgcat gtgggtggcg ctgttctcga tcgtcgtggt gctgctcccg gtctcgcggc
     1741 tggtgccgcc gctgtaccgc ttccgggtgc gctcgcgcgt gtttcgctgg taccggcagc
     1801 tgcgccagat cgaggacgcc gtgggcagcc gcgacccgca agagctgcgc gccgagctcg
     1861 acaagcttga cgcgcgcgcg gcgcgcatcg tggtgccgct ctcctatgcc gacgagctct
     1921 actcgctgcg ctcgcacatc gagctggtgc gcgctcgcct gcaaaaggaa aatgctagag
     1981 ttacgcgatg acgaaaccgc gcgcgaggct gcggcatttt gcggtggtgg tacgcgatct
     2041 cgagagagcc gcggcgttct acgaaaaggt gttcggcctg cggcgcgtcg gccgcgaaga
     2101 cctcgagatg ggatcgggca tctatctgag cgacggcgtg atcaacctcg cgctcctcaa
     2161 gtacaagggc gagtccggct cagggttgaa ggacgcggcc agcttcgtcg gcgcgcatca
     2221 ttttggcttc caggtcgacg accttgaggc ggcgcgcaag cgcatcgagg cggccggcgg
     2281 aacatttttc ttcacgctcg gcaagaccaa ggacgatgcc aatttcgaga tgaagttcaa
     2341 ggaccccgac ggagtgatct tcgatatctc cgagaagggc tggagcggcg gcccctaggc
     2401 gcttccggtc ggaaggtgcc tcgcggtccg cgtgcgctgg cgcgtcagcc accgcgagcg
     2461 cccggcgacc gaataaaaga gcgcgcgcag cccgggaggc cagaacgtgg gcatgccgaa
     2521 aaagagcgag atgcgctgcg tgtgattggt gaggggccgc tctgcgcgct cccatgcatc
     2581 gagcgcagac ggtacgtcgt gacctcgctc caggaacacg cccagcgaca gcgcattcat
     2641 catcgcgcag cccgctccct gcccgatatt cggcgggatc gcatgcgccg cgtcgccgat
     2701 cacagccacc ctgcccgacg accagcgctt gaggcgaatg tactcgaagc gatcgtagcg
     2761 gccgtcgtcg ccgatgcggt cgatgagcgg cgcgagccgg ggaaacgaac gcttccagac
     2821 tgccttgtcg acgggcagcg agcgcgccgc gtcgtcctcg tggagcatcg agagcgcgag
     2881 atagaggtgc gtcgtgctgc acgggttgta gagcagccgg cggctgcccg accagtactc
     2941 gatggtcttg ccgtccgtgc cctcctcgac cgtccgcgtc gggatcagca ggcgaatgca
     3001 gccgtcccgc agatagcgcc ggcggctgac caggccgagc gaatcgcgga tctgcgaatt
     3061 gacgccgtcg gcggcgacga tcaagtctgc cttgagccgc cgtccgtcgg cgagcgtgag
     3121 ctcgcccgac cgcgtggccg aaacgccttc ggagtcggtc atgatctccg cgcccgcccg
     3181 cagcgcggcc accgccaagg catcgatcat ccgctgccgc aagacgccgt agacgcgaat
     3241 atcccaggga tgcgtcgaca acagccggtc gtgctgatcg cgagtttcgc gcagcctggc
     3301 tcgggccccg tcgcgtatcg tgtcctggta cgcaccgagc gcctcgagca cgcgcaagcc
     3361 gttctcgtag acgttgattc cagcgcctgc ggtgcgcagc cggtcggcgc gctcgtgcag
     3421 ccgcacgcgc cagccgcgcc gggcgagcgc gcaggccgcg gtcaatcctg cgaaaccgcc
     3481 gcctacaatt tctaccttgc ctttcacctg ctccagaata cgcgatagcc gccatgctcg
     3541 acgaacgcat cgccaggttt ccccgcagga aatcgaaggc catctcctac cgcgaggcgg
     3601 gcgcggggca ggcgctggtc ctgctgcacg gcatcggctc gagctccgcg agctggctgt
     3661 tccagctcga gagcctgaag ggttaccggc tgatcgcctg ggacgccccc ggctacggtg
     3721 attctgattt tttgaatgaa aagaacccca gagcggagaa ctacgccgcc gctctgcacg
     3781 gcttcctcga gcgcttgctc ctcaaggacg tggtgctggt cggcagctcg ctcggctgcc
     3841 tgatggccgc ggcctatgcg gccgcacatc cggagcgcgt gcgcagcatg ctgcttctcg
     3901 ggccggcggc cggtttcgcg ggcgaccaga cgaaactgga agaaaggctc gcggagatcg
     3961 accagctcgg ccccgaaggc atggcggaga agcgctcgcc gaccctggtc gcgaaggcat
     4021 cgccgcc
//
LOCUS       DASPXW010000090         2713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_532387, whole genome shotgun sequence.
ACCESSION   DASPXW010000090 DASPXW010000000
VERSION     DASPXW010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532387"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VEL04_02145"
     CDS             complement(<1..511)
                     /locus_tag="VEL04_02145"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYT47199.1"
                     /translation="MLIDGRASVAQIIKAAAPSIAPEAVLVVLEKLQKAGHIAAAAGV
                     ASGGIDAGNFFTRPVAPGAAGAQVASESEAGLASLQEQGYFVRIARRGAARKLAKDEK
                     LTVLVVEDDPQLAKLLRTYLAMEGLVPRLAANRGEIEAALSQAPKPDLVLLDVTLPDV
                     DGFEVLARIR"
     gene            741..887
                     /locus_tag="VEL04_02150"
     CDS             741..887
                     /locus_tag="VEL04_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47200.1"
                     /translation="MPAGSFVVHHANQIHYDGAKDEEVLLQIIGVGPSATIRVDESGQ
                     PKPF"
     gene            complement(884..1909)
                     /locus_tag="VEL04_02155"
     CDS             complement(884..1909)
                     /locus_tag="VEL04_02155"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47201.1"
                     /translation="MNQLLPTTVVGSYPQPGWLIDRAKLGSKVPRVRAPEIWRMAGPL
                     LEEAQDDATLLAIRDMERAGIDIITDGEMRRESYSNRFATALEGVDIENPGTTVNRTG
                     ARSVVPRISGPIRRIRPVEVQDLLFLKSHTSRATKITLPGPFTMAQQAQDDHYRDEEA
                     LALAFAAAVNEEMRDLKAAGADVIQLDEPWLQARPERAMRYGVKAINRALEGIPGTTV
                     VHLCFGYAAAVSDKPSGYSFLPQLADSSASQISIEAAQPRLDLAVLRELGKKTVMLGV
                     IDLGNPAVETPEAVAGRIRAALKHVPAERLVAAPDCGMKYLPRDTAFGKLAALVQGAA
                     LVRRSLG"
     gene            1949..>2713
                     /locus_tag="VEL04_02160"
     CDS             1949..>2713
                     /locus_tag="VEL04_02160"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF005089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HYT47202.1"
                     /translation="MKICVFGAGAVGGHIAARLAAAGNEVSVVARGEHLAAMKEKGLR
                     LLHGEETIRGRVRAAERSADLGRQEVVFVALKANLLALFAEHAAPLLGAETPVVFAQN
                     GIPWWYGIGRQAPDLSPLDPGGKLARVIAPERIMGGVIYSANEVIEPGVILNHVPGRN
                     MLVIGEADDRDSSRIKTLRNILEQSGMSSPPAPDIRAAIWSKLLQNLGNSTLCTLTGA
                     TVAELRGDAGLGKLAARIGAEGRAIAQAHGIDLEAAP"
BASE COUNT          429 a          902 c          944 g          438 t
ORIGIN      
        1 cgcggatgcg ggcgagcacc tcgaagccgt cgacgtcggg cagcgtcacg tcgagcagca
       61 ccaggtcagg cttgggcgcc tggctcagcg cggcctcgat ctccccccgg ttcgccgcga
      121 ggcggggcac caggccttcc atcgccaggt aggtgcgcag cagcttggcg agctgcgggt
      181 cgtcctcgac caccaggacc gtgagcttct cgtccttggc gagcttgcgc gcggcgccgc
      241 gccgcgcgat gcgcacgaaa tagccctgct cctgcagcga cgccagcccg gcttccgact
      301 cggaagccac ctgcgcgccc gcagcgcccg gtgccaccgg ccgggtgaag aagttgccgg
      361 cgtcgatccc ccccgaggcg acgccggcag cggcggcgat gtgcccggct ttctgcagct
      421 tttcgagcac cacgagcacc gcttcgggcg caatcgatgg cgccgcggcc ttgatgatct
      481 gcgccacgct cgcgcgcccg tcgatcagca cgagcagctt gagctccgca gtggagagcg
      541 aggtgccggc ggcggcgttg agctccgctt tcccgttact ggtgagggcg tagacggctt
      601 tctggtccag ctggctcctc ccgagagttt cactataccg ttgatgcgca acgtcagctt
      661 tttgtcaggg gcggcggctt agcaaaggga cctggtggat cggctccggt ccgaaatggg
      721 acaaggaagc gaccaccccg gtgcccgccg gcagcttcgt cgtgcatcac gcgaaccaga
      781 tccactacga cggcgcgaag gacgaggaag tgctgctgca gatcatcggc gtcggcccga
      841 gcgcgaccat ccgggtcgac gagtccggac aaccaaagcc cttctagccg agcgagcggc
      901 gcaccagcgc cgctccctgc accagggcag ccagttttcc gaaggcggtg tcgcgcggca
      961 ggtatttcat gccgcagtcg ggcgcggcga ccaggcgttc agccggaaca tgcttgagcg
     1021 cggcgcgaat gcgcccggcc accgcttcgg gcgtttcgac tgcggggttg ccgaggtcga
     1081 tgacgccgag catgacggtt tttttcccca gttccctcaa cacagccaga tcgagcctgg
     1141 gctgcgccgc ctcgatcgag atctgcgagg cgctcgagtc tgcgagctgc ggcaggaagg
     1201 aatagcccga gggcttgtcg ctgaccgccg ctgcatagcc gaagcacagg tgcacgacgg
     1261 tggtgccggg tatgccttcg agcgcgcggt tgatcgcctt gacgccgtag cgcatcgcgc
     1321 gctcggggcg cgcctgcagc cacggctcgt cgagctggat cacgtcggcg ccggcggcct
     1381 tcaggtcgcg catctcctcg ttcaccgccg cggcgaaggc gagggcgagc gcttcctcgt
     1441 cgcggtaatg gtcgtcctgc gcctgttgcg ccatggtgaa cggacccggc agggtgatct
     1501 tcgtcgctct ggaagtatgg cttttcaaaa atagaaggtc ctggacctcg acgggacgga
     1561 tgcggcgaat cgggcccgag atgcgcggta ccaccgagcg cgccccggtg cggttgacgg
     1621 tggtgccggg gttctcgatg tcgaccccct cgagcgcggt ggcgaagcgg ttcgaatagc
     1681 tttcgcggcg catctcgccg tcggtgatga tgtcgattcc cgcgcgctcc atgtcgcgga
     1741 tggcaagcag cgttgcgtcg tcctgggctt cctccagcag gggtcctgcc atcctccaga
     1801 tctccggggc tcgcacgcgc ggcaccttcg agccgagctt cgcgcggtcg atcagccagc
     1861 ccggctgcgg atagctgccg acgacggtgg tgggaagaag ctgattcact tgtacgatct
     1921 tacatggagt gtttaggctg gaaaccaaat gaaaatctgc gtattcggcg ccggcgcggt
     1981 cggcggccac atcgccgcaa ggctcgcggc ggccggcaac gaagtgtcgg tggtggcgcg
     2041 aggcgagcac ctcgcggcca tgaaggagaa aggcctcagg ctgctgcacg gcgaggagac
     2101 catccgcggc cgcgtgcggg cagccgagcg ctcggccgac ctcggcaggc aggaggtggt
     2161 cttcgtggcg ctgaaggcca acctgcttgc gctgttcgcg gagcatgctg cgcccttgct
     2221 gggcgccgaa acgccggtgg tcttcgcgca gaacggcatc ccgtggtggt atggcatcgg
     2281 caggcaagcg cccgacctgt cgcccctcga ccccggcggc aagctcgcgc gcgtcatcgc
     2341 gcccgagcgc atcatgggcg gcgtgatcta ttcggcgaac gaagtgatcg agcccggggt
     2401 aatcctgaat cacgtgcccg ggcgcaacat gctggtgatc ggggaagccg atgaccggga
     2461 ttccagcaga ataaaaacgc tgcggaacat actcgagcaa tccggcatgt cgtctccccc
     2521 ggcgccagac atccgcgccg cgatctggag caagctgctg cagaacctcg gcaactcgac
     2581 cctgtgcacg ctcaccggcg ccacggtggc agagctgcgc ggcgacgcgg ggctcggcaa
     2641 gctcgccgcg cgcatcggcg ccgagggccg cgccatcgcg caggcgcacg gcatcgacct
     2701 cgaggccgcg ccg
//
LOCUS       DASPXW010000091         3752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1037717, whole genome shotgun sequence.
ACCESSION   DASPXW010000091 DASPXW010000000
VERSION     DASPXW010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037717"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..856)
                     /locus_tag="VEL04_02165"
     CDS             complement(140..856)
                     /locus_tag="VEL04_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014749681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HYT47203.1"
                     /translation="MSVLYSVSQGVATLALNRPEARNALNLEMCEALLEVTKNLLEKE
                     VRVVFVRGNGPVFCGGADLKERGGKSEAWVRARRMKAFAAYGALEALPMPCVALVHGA
                     AIGSGCEIAAACDFIVATPEATFRTPEAQRGTVGATQRLPRILGKRLAKDMMFTGRRL
                     SAAEAREAGLVSRLLQKDKLEVEIAQIAAAIAAAPPAAVALAKRCINRTVELDRKGAL
                     QVEIEAIDEQLASGTWLGKK"
     gene            complement(853..1989)
                     /locus_tag="VEL04_02170"
     CDS             complement(853..1989)
                     /locus_tag="VEL04_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HYT47204.1"
                     /translation="MKPLEHVTILDLSRVLACPFASMILAELGARVIKVEQPGSGDET
                     RSFEPQLAADSAYYFACNRSKESITVDLRSAEGKKIIRELAQKADVLVENFPVGTLGR
                     HALDFASLGALNEKLIYVSCTGFGQSGPYAQKKGYDTVFQAMGGIMSLTGERGGPPVK
                     PGLPVADLTSGLWVAIAILAALAGRSRSGKGCQVDFSMLDGQVALLTLAAARYFALGE
                     IPPRLGTEHPGRVPSATFRCKDGKYAHITASDQHWMPLCKALGLEQWGLPFDSNSIRV
                     DRREEIMAVLTREIQKLPRAELLEKLDAADVPVGPVNDVAEILRDPHVRARRLVGSFD
                     YPGVGEFRALALPYKFLGWDNPEIGRPPLLGEHTGKILKELGLA"
     gene            complement(1986..2714)
                     /locus_tag="VEL04_02175"
     CDS             complement(1986..2714)
                     /locus_tag="VEL04_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006385514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HYT47205.1"
                     /translation="MNLLVEDKGAVRILTLNRPEKRNALDTALSRELLGALRAADADE
                     TVRCVVLTGAGPAFCAGADLAEFKGWSDPNAADTRAELTMNLHLIFSKMITPVVTAIN
                     GAAMGGGAGLAIAGDLAVMAQSAKLGYPETRHGIVAAIVMANLVRQVGRKAAFELVAL
                     AEPIAAARALELGMVNRVVPSSKLMKEALSLAGKLAAVARPAMAQTKKLFHEVADLAL
                     PQALERGRDANKRMRNFEKLDRKK"
     gene            complement(2711..3655)
                     /locus_tag="VEL04_02180"
     CDS             complement(2711..3655)
                     /locus_tag="VEL04_02180"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HYT47206.1"
                     /translation="MKTRITELLGIEHPIFQAAMSWASSNSALVIAVSNAGGMGVLAA
                     GPLRLDDFSRVLKEIRNGTSKPFGLNIPLNNPKAGEMLDLAYQARIPVMVASQGGPRE
                     HLARFRGIGTKWLHVVASVEHAKKAEAAGVDALVVDGAEAGGHPPPSEVGTLVLVRRV
                     LQAVKLPVVASGGVADGAGVAALLALGADAVQLGTRFIATPEASVHDNYKRAVLDAEV
                     DQTTLVGRGMHPIRQLKNAFSAKYQAAERAGASRDELETLFQEYSLKQAALRGDIEWG
                     KVEAGQSAGLVHEILPAAEVMRRLVNELEAARQRLAKL"
BASE COUNT          584 a         1295 c         1200 g          673 t
ORIGIN      
        1 aggccgagcg cctgcatcgc gcccgcggcg ctctgcgctt tttcgaacag ttccttgcat
       61 gaacaacggg ccaccggcgt gatcagcgcc tcggcgtcag gccgctcgag ggaatttctt
      121 ttcaggaact ccccgaggat catttcttgc cgagccaggt tccgctggcg agctgctcgt
      181 cgatcgcctc gatctcgacc tgcagcgcgc ccttgcggtc gagctccacg gtgcggttga
      241 tgcagcgctt ggcgagcgcg acggccgccg gcggtgctgc ggcgatggcg gccgcgatct
      301 gcgcaatttc gacttctaat ttgtcttttt gcaaaaggcg ggacaccagc cccgcttcgc
      361 gcgcctctgc ggcgctcaac cgccgtccgg tgaacatcat gtccttggcg aggcgcttgc
      421 cgagaatgcg cggcaggcgc tgcgtggcgc cgaccgtgcc gcgctgcgct tcgggggtgc
      481 ggaacgtcgc ttccggcgtg gcgacgatga agtcgcaggc cgcggcaatc tcgcagcccg
      541 agccgatcgc ggcgccgtgc accagcgcga cgcacggcat cggcagcgcc tcgagcgctc
      601 catacgcggc gaacgccttc atgcgccgcg cgcgcaccca tgcctcgctc ttgcctccgc
      661 gctccttgag gtcggcgccg ccgcagaaca ccggcccgtt gccgcgcacg aagaccacgc
      721 gcacctcctt ttcaagcaga ttttttgtaa cttccaacag cgcttcgcac atctccaggt
      781 tgagcgcgtt gcgcgcctcc gggcggttga gcgcgagggt ggcgacgcct tgcgacacgg
      841 aatacaggac gctcatgcca ggccgagctc cttgaggatt ttcccggtgt gctcgcccag
      901 gagcggcggg cggccgattt ccggattgtc ccagccgaga aacttgtacg gcagcgcgag
      961 cgccctgaat tcgcccacgc ccgggtaatc gaagctgccg acgagccgcc gcgcgcgcac
     1021 gtgcggatcg cgcaggatct cggcgacgtc gttgaccggc ccgaccggga catccgcggc
     1081 atcgagcttt tccagcagct cggcgcgagg gagcttctgg atttcacggg tcaataccgc
     1141 catgatctct tcgcgccggt cgactcgaat cgagttggaa tcgaacggca aaccccactg
     1201 ctccaacccg agagccttgc acagcggcat ccagtgctgg tcgctcgccg tgatgtgggc
     1261 gtacttgccg tccttgcagc ggaaggtcgc cgacggcacg cggccggggt gctcggtgcc
     1321 cagtcgtgga ggtatttcgc ccagggcgaa atacctggcg gcagccaagg taagcagcgc
     1381 gacctgcccg tcgagcatcg agaaatcgac ctggcagccc ttgccgctgc ggctgcggcc
     1441 ggcaagcgcg gcgaggatcg cgatcgccac ccacagcccc gaggtgaggt cggcgaccgg
     1501 caggccgggc ttcaccggcg ggccgccgcg ctcgccggtg aggctcatga tgccgcccat
     1561 cgcctggaac acggtgtcgt agcctttttt ctgcgcatag ggaccggatt gaccgaagcc
     1621 ggtgcaagac acatagatca atttttcatt taatgcaccg agcgaggcga aatcgagggc
     1681 atggcggccg agcgtgccga cgggaaagtt ctcgaccagc acgtccgcct tttgcgcaag
     1741 ctctctgatg attttcttgc cttctgcgga acgcaggtcg accgtgatgg attccttcga
     1801 gcggttgcag gcgaagtaat aggcgctgtc ggctgcgagc tgcggctcga agcttcgcgt
     1861 ctcgtcgccg ctccccggct gctcgacctt gatcacgcgc gcgccgagct cggcgaggat
     1921 catcgaggcg aacgggcagg cgagcacgcg cgacagatcg aggatcgtca catgctctag
     1981 cggcttcatt ttttcctgtc cagcttctcg aaattgcgca tgcgcttgtt ggcatcgcgg
     2041 ccgcgctcga gcgcttgcgg cagcgcgagg tcggccacct cgtggaacag cttcttggtc
     2101 tgcgccatcg ccggccgggc gactgcggcg agctttcccg ccagcgacag ggcctccttc
     2161 atcagcttcg atgaaggcac gacgcggttc accatgccga gctcgagcgc gcgcgcagcc
     2221 gcgatcggct cggcgagcgc caccagctcg aaggcggcct tacggccgac ctggcgcacc
     2281 aggttggcca tcacgatcgc agccacgatg ccgtgcctgg tctcgggata gccgagcttc
     2341 gcgctctggg ccatgaccgc gagatcgccg gcgatggcga ggcccgcgcc gccgcccatc
     2401 gcggcgccgt tgatcgccgt aaccactggc gtgatcatct tggagaagat gagatgcaga
     2461 ttcatcgtca gctcggcgcg cgtgtcggcg gcattgggat ccgaccatcc cttgaactcc
     2521 gcaagatcgg cgccggcgca gaatgccggc cccgcgccgg tgagcacgac gcagcgcacc
     2581 gtctcgtcgg catccgcggc gcgcagcgcc ccgagcagct cgcggctgag cgccgtgtcg
     2641 agcgcgtttc tcttctccgg gcggttgagc gtgagaatgc gcaccgcccc tttatcttcg
     2701 accagcaggt tcatagtttc gccaggcgct gacgcgccgc ctccagttcg ttgaccaggc
     2761 ggcgcatcac ttccgccgca ggcaggatct cgtgcaccag gcccgcgctc tgcccggctt
     2821 ccaccttgcc ccattcgatg tcgcccctga gcgcggcctg cttcaatgaa tattcctgga
     2881 acagcgtctc gagctcgtcg cgcgatgcgc cggccctctc ggctgcttgg tacttggcgg
     2941 agaacgcgtt cttcagctgc cggatcggat gcatgccgcg cccgacgagc gtggtctgat
     3001 ccacttcggc atcgagcacc gcgcgtttat agttgtcgtg cacgctcgcc tcgggcgtgg
     3061 cgatgaagcg cgtgccgagc tgcaccgcat cggcgccgag cgcaagcagc gccgcaacgc
     3121 cggcgccatc ggccacgccg ccgctcgcca ccaccggaag cttcaccgcc tgcagcaccc
     3181 gccgcaccag caccagcgtt cccacttccg acggcggcgg atggccgccc gcctccgcgc
     3241 cgtcgaccac cagcgcatcg acgcccgcgg cttcggcttt cttcgcgtgc tcgaccgacg
     3301 ccaccacgtg cagccacttc gtgccgatgc cgcggaagcg cgccaggtgc tcgcgcgggc
     3361 cgccttgcga ggcgaccatc accggaatgc gagcctggta cgcgaggtcg agcatttcgc
     3421 cggccttcgg attgttcagg ggaatgttga gtccgaaggg tttcgaggtg ccgtttctta
     3481 tttcctttaa tacccgtgaa aaatcgtcta gccgcagcgg tcccgcggcg agcacgccca
     3541 tacctccggc gttcgacacg gcgatcacca gcgccgagtt ggacgaggcc cagctcatcg
     3601 ccgcctggaa gatagggtgc tcgatgccga gcagctcggt gatgcgcgtc ttcaatgttt
     3661 ttcctgttga atgaaaaccc tggcctgcat cgcgagcttg accagcgcgc gctcgaagac
     3721 ctgcttttcc ctctgcgaca ggcagcccct ga
//
LOCUS       DASPXW010000092         2763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1038353, whole genome shotgun sequence.
ACCESSION   DASPXW010000092 DASPXW010000000
VERSION     DASPXW010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038353"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..977
                     /locus_tag="VEL04_02185"
     CDS             108..977
                     /locus_tag="VEL04_02185"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYT47207.1"
                     /translation="MLVVDCDVPWIPKEGKPRAQAKVIHIGTDPLFARYPLRGFRTDI
                     ALTGAVAPTLQALRRSAQKQVVSPKQLEERRKAVSQLSSDIRSKGRSGFEPMPANITG
                     KWLSACMNRLLDDQTILINEYPIVLEEMVIKEPGRYFGNAAAGGLGWGMGAALGAKLA
                     SPDRTVICALGDGSYMFGNPTATHYVSEAMRLPVFFVIANNARWAAVHRSTLSTYPKG
                     FASENKQPPFATLEPSPRFEHVIQASGGHGERVSEPKELMPALQRALKVVKEEKRQAL
                     VNVCLEASYVKTS"
     gene            complement(984..1736)
                     /locus_tag="VEL04_02190"
     CDS             complement(984..1736)
                     /locus_tag="VEL04_02190"
                     /inference="COORDINATES: protein motif:HMM:NF023984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3750 domain-containing protein"
                     /protein_id="HYT47208.1"
                     /translation="MLLFLSTAASAAAQDWRAASLEPVGLAPNPRTTPEAVVQVYGAR
                     TRGLKGLFGVHTWVAVKATDASAWTVYEVIGWRLRYSDSAVVVRTRDPDGRWFGSEPE
                     LYAEKRGPGVDELIRRVDQAARSYPYAKEYTLWPGPNSNTFTAWIGRAVPELELDLPA
                     TAIGKDYIGSSVVSRAPSGTGFQFSLGGVLGLTASKVDGVELNILSLNFGVGPSGLKL
                     PLIGRIGTPHFGAAVAAPAAGAAAAAEAPLQP"
     gene            complement(1757..2548)
                     /gene="ttcA"
                     /locus_tag="VEL04_02195"
                     /pseudo
     CDS             complement(1757..2548)
                     /gene="ttcA"
                     /locus_tag="VEL04_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
     gene            complement(2545..>2763)
                     /locus_tag="VEL04_02200"
     CDS             complement(2545..>2763)
                     /locus_tag="VEL04_02200"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYT47209.1"
                     /translation="PRVRVNGVAPGAVAWPEDGQLESAERSRILATTPLGRMGTPEDI
                     AQAVHFLACAPYITGQILAVDGGRSIFL"
BASE COUNT          502 a          883 c          936 g          442 t
ORIGIN      
        1 gcctgcatca accagctgct cggcgaggac tcgatcctcg tcaacgaata cccgatcgtg
       61 ctcgaggaga tgacgctccg cacgccctgc tcaaggaagc ggacgtagtg ctggtcgtgg
      121 actgcgacgt gccgtggatc ccgaaggaag gcaagcccag ggcgcaggcc aaggtgatcc
      181 acatcggtac cgacccgctg ttcgcgcgct acccgctgcg cggcttccgc accgacatcg
      241 cgctgaccgg cgccgtggcg ccgacgctgc aggcgctccg gcgcagcgcg cagaaacagg
      301 tcgtctcgcc gaagcagctc gaggagcggc gcaaagcggt ttcacaatta tccagtgaca
      361 taagaagcaa aggccgcagc ggcttcgagc cgatgcccgc gaacatcacc ggcaagtggc
      421 tctcagcgtg catgaaccgc ctgctcgacg accagacgat cctgattaac gagtacccca
      481 tagtgctgga agaaatggtg atcaaggagc cgggccgcta tttcggcaat gccgcggcgg
      541 gaggcctggg ctggggcatg ggcgccgcgc tcggcgccaa gctcgcctcg cccgacagga
      601 cggtaatttg cgcgctcggc gacggctcct acatgttcgg caacccgacc gcgacgcact
      661 acgtctcgga ggcgatgcgc cttccggtgt tcttcgtcat cgccaacaac gcacgctggg
      721 cggcggtgca caggtccacg ctctcgacgt atccgaaggg atttgcttca gaaaacaaac
      781 aacccccctt cgccacgctg gaaccctcgc cgcgcttcga gcacgtgatc caggcctcgg
      841 gcggccacgg cgagcgcgtc agcgagccga aggagctgat gccggcgctg cagcgcgcgc
      901 tcaaggtggt gaaggaagag aagcggcagg cgctcgtcaa cgtctgcctc gaggcgagct
      961 acgtcaaaac cagctagtcg caatcagggt tgcagcggcg cttcggcggc ggccgcggca
     1021 cccgcggccg gggcggcgac ggcggcgccg aagtggggcg taccgatccg gccgataagc
     1081 ggcagcttaa ggccagaggg gccgacaccg aagttgaggc tcaggatgtt gagctccacg
     1141 ccgtcgacct tgcttgcggt gaggccgagc acaccgccga gggaaaactg gaatccagta
     1201 ccgctcggtg ccctcgagac gaccgagctg ccaatgtagt ccttgccgat agcggtggcc
     1261 gggagatcca gctctagctc gggcaccgcg cgcccgatcc aggcggtgaa ggtattggag
     1321 ttgggcccgg gccacagggt gtattccttg gcatacgggt agctgcgcgc ggcctggtcc
     1381 acgcgccgga tcagctcgtc gaccccgggg ccgcgctttt cggcgtacag ctcgggctcg
     1441 gagccgaacc agcggccgtc ggggtcgcgc gtgcgcacca ccacggccga atcggagtag
     1501 cgcagccgcc agccaatgac ttcgtacacg gtccatgcgg aggcatcggt cgccttaacc
     1561 gccacccagg tgtgcacgcc gaacaagccc ttcaagccgc gggtgcgcgc gccgtagacc
     1621 tggacgaccg cctcgggcgt ggtccgggga ttgggtgcaa ggccaaccgg ctcaaggctc
     1681 gcggcgcgcc agtcctgagc tgcggccgac gctgcggtag agagaaaaag cagcatgacg
     1741 atagaacgca tattcaaggt tgcaggttga cgaagtcgaa gagcttgcgg tcgagcaggt
     1801 gcgaccggct cacgtcggtg agggcgcgca gaatcgactc aatgcgcccg gggtggcgtt
     1861 tctgccattc ccgcagcatc tcgcccacca gctttctttg caggttctcc tgcgagccgc
     1921 acagcgtgca cggaatgatg ggaaagacct tcatctcggc atattcgatc agatcttctt
     1981 ctgccacata ggcgagcggc cggatgacca catgcttgcc gtcgtccgac agcagcttcg
     2041 ccggcatcgc cttcagcttg ccgccgtgga acaggttgag aaagaaggtg gcgaggatgt
     2101 cgtcgcgatg gtgaccgagc gcgatcttgg tggcgccgag ctcgcccgcg acgcggtaga
     2161 gcgcgccgcg ccgcagccgc gagcacagcg agcacatcgt gcttccctcg gggatcacgc
     2221 gcttcacgac cgagtaggtg tcctgctcga cgatgcggta gggcaccccg cgcgcctcga
     2281 ggtatccggg tagcacctgc gccggaaagc cgggctgctt ctggtcgagg ttgaccgcga
     2341 tcagatcgaa gttgacaggt gccttggttt tcaagctgat aagcaaatcg agaagagcgt
     2401 ggctgtcctt gccgcctgag aggcacacca tgacgcggtc gccctgctcg atcatgctga
     2461 agtcggcgat cgcctcgccc acctgccggc gcaggcgctt cgcgagcttg gcgtgctcgt
     2521 attcgatttt tcgaaaatcg tctttcatag gaagatcgag cgaccgccgt ccacggcaag
     2581 aatctgcccg gtgatgtagg gcgcgcaggc gaggaagtgt accgcctggg cgatgtcttc
     2641 gggcgtgccc atgcggccga gcggggtggt ggcgaggatg cggctgcgct cggcggactc
     2701 gagctggccg tcctcgggcc aggcgacggc gccgggcgcg acgccgttca cccgcactcg
     2761 cgg
//
LOCUS       DASPXW010000093         2993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_785279, whole genome shotgun sequence.
ACCESSION   DASPXW010000093 DASPXW010000000
VERSION     DASPXW010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785279"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VEL04_02205"
     CDS             <1..236
                     /locus_tag="VEL04_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYT47210.1"
                     /translation="KMPKVDGIEVLRRLKADPQTRPVPVVVMTSSNEERDVVESYRLG
                     VNSYIVKPVQFDAFLETVAKIGLYWVLTNRVPQ"
     gene            233..2431
                     /locus_tag="VEL04_02210"
     CDS             233..2431
                     /locus_tag="VEL04_02210"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HYT47211.1"
                     /translation="MTTAPPIRVLLTEDVLSDAELEVRELKRAGLRIAHRIADTEQAF
                     AVALLEFDPDVILSDFSMPAFDGMAALALARSLRPETPFIFVSGTIGEEYAIRALKNG
                     ATDYVLKTNLVRLPAAVERALAEARQRQRITRLSRIREFSSGINAALVRLRERQPLFE
                     EICRIAVEVGGFKGAHVGRADPETGEVRWVCQHGWITQPTEVPVSARADVEAGQGLVG
                     MALRNAKPAIWNDVRHEPGVRNREHFLAHGVAAVGTFPLMVEAQPIGVFSLHAGEPGF
                     FDQEEIALLEELASNVSFALELMAKQERLNYLAYYDALTELPNHALFHDRLTRAIESA
                     KSSGKKMALMVLDLERFKAINDTLGRQAGDRVLQAVARRLHEAVGDLNRVARLGGNTF
                     AVMFPDIAGAEDAARRLEKDPAQFFGLPFEIDEREVRLAVKAGIAVYPADGPDADALF
                     RNAEASLKQAKERGERCLFYTPQINARLAEQLELEHRLRKAVEHGELFLHFQPKYDLL
                     DRRIVGLEALMRWQSPDGCGLVSPAKFVPVLEQTGLILEAGRQVIAGATAKYRDWRGR
                     GLKAPRIAVNVSALQLRRKSFVQDVQAALGESSADGGGIDLEITESLLMTEADESIRK
                     LRELREMGMQLALDDFGTGYSSLAYLSKLPVDSLKIDRGFVRGMTEKADDTSIVSAII
                     SLAQALRLKVIAEGVETEQQAQLLRLLRCDQAQGYLFSPPVPAEKLETLL"
     gene            2458..>2993
                     /locus_tag="VEL04_02215"
     CDS             2458..>2993
                     /locus_tag="VEL04_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HYT47212.1"
                     /translation="MSLYALGMNHQTAPLAVRERVVFHVERLREALTEIKGALAREAA
                     ILSTCNRTELYVAGEQPARLAQWLARYHRLEPNELSPYLYTLPSEQAVRHAFRVASGL
                     DSMVLGEPQILGQMKDAARAAESAGTLGTVLHKLFQRSFAVAKEVRSTTRLGAQSVSM
                     AAAAVKLAARIFPSLKDQ"
BASE COUNT          529 a          956 c         1003 g          505 t
ORIGIN      
        1 tcaagatgcc gaaggtcgat ggcatagaag tgctgcggcg gctgaaggca gatccgcaaa
       61 cacgccccgt tccggtggtg gtaatgacct cgtcgaacga ggagcgcgac gtggtggaaa
      121 gctaccggct cggcgtcaac agctacatcg tcaagccggt gcagttcgac gcatttctcg
      181 agacggtagc caagatcggg ctgtattggg tgctcaccaa ccgcgtgccg caatgaccac
      241 ggcgccgccc atccgggtgc ttctgaccga ggacgttttg tcggatgccg agctcgaggt
      301 gcgcgagctc aagcgcgcgg ggctgaggat cgcgcaccgc atcgccgaca ccgagcaggc
      361 gttcgccgtg gcgctgctcg agttcgatcc cgacgttatt ttgtcggact tctcgatgcc
      421 cgccttcgac ggcatggcgg cgctcgcgct cgcgcgctcg ctgcgtccgg agacgccgtt
      481 catcttcgtc tccggcacca tcggcgagga atacgccatc cgcgccctca agaatggcgc
      541 taccgattac gtgctcaaga ccaacctggt gcggctgccg gccgccgtcg agcgcgcgct
      601 cgccgaggcg cgccagcgcc agcgcatcac gcggctgtct cgcatccgcg aattctcgag
      661 cgggattaac gctgcgctgg tccgcttgcg tgagcgtcag ccgctattcg aggagatctg
      721 ccgcatcgcc gtagaagtcg gcggtttcaa gggcgcacac gtcggtcgcg cggatccgga
      781 gacaggagaa gtgcggtggg tctgccagca cggatggatt acgcagccca cggaggtccc
      841 ggtctcggcg cgagcggacg tggaggcggg ccaagggctc gtaggaatgg cgctgcgcaa
      901 cgcaaaacct gcgatctgga acgacgtgag gcacgaaccc ggggtaagga accgagagca
      961 ttttctcgcg cacggcgtcg cggcggtggg cactttcccg ttgatggtcg aagcacaacc
     1021 gatcggggtt ttcagcctgc acgccggcga gcccggattc ttcgatcagg aggagatcgc
     1081 gctgctcgaa gagctcgctt cgaacgtgtc gtttgcgctc gagctgatgg ccaagcagga
     1141 gcggctcaac tacctcgcat actacgacgc gctgaccgaa ttgccgaacc acgccctgtt
     1201 ccatgaccgc ttgacccgcg cgatcgagag cgcgaagagc tcaggcaaga agatggcgct
     1261 tatggttctc gacctggagc gcttcaaagc gattaacgac accctaggcc ggcaggctgg
     1321 cgaccgcgtg ctgcaggctg tggcgcgccg tctacatgaa gcggtcggcg accttaaccg
     1381 cgttgcgcgc ctcgggggaa acacgttcgc ggtgatgttt ccggacatcg caggtgccga
     1441 ggacgccgcg cggcgtctcg aaaaagatcc agcccagttc ttcggcctgc ctttcgagat
     1501 cgacgagcgc gaggtccggc tcgcggtgaa ggccggcatc gcggtctatc cagccgacgg
     1561 ccctgatgcc gacgcgctgt tccgcaatgc cgaggcgtcg ctgaagcagg cgaaggaaag
     1621 gggcgagcgc tgcctgttct acacgccgca gatcaacgcg cgcctcgccg agcagctcga
     1681 gctcgagcat cggctgcgca aggcggtcga gcatggcgag ctgttcctgc acttccagcc
     1741 gaaatatgac ctcctcgacc ggcgcatcgt cgggctcgag gcgctgatgc gctggcagtc
     1801 gccggacggt tgcgggctgg tctcacccgc gaagttcgtg ccggtgctcg agcagaccgg
     1861 cctgattctc gaagccgggc gccaggtcat cgccggcgcc acggcgaagt acagggattg
     1921 gcgggggcgc ggcctgaaag cgccacgcat tgcggtcaac gtctcggcat tgcagctgcg
     1981 ccgcaaaagc ttcgtccagg atgtgcaggc ggcgctcggc gaatcgagcg ccgacggcgg
     2041 cggaatcgac ctcgagatca ccgagagcct gctgatgacc gaggccgatg agagcatccg
     2101 caagctccgc gagctgcgcg agatggggat gcagctcgcg ctcgacgact tcggcaccgg
     2161 ttactcgtcg ctcgcgtact tgagcaagtt gccggtcgac tcgctgaaga tcgaccgcgg
     2221 cttcgtccgc ggcatgaccg agaaggccga tgacaccagc atcgtctcgg ccatcatttc
     2281 gctcgcgcag gcactgcggc tcaaggtgat tgccgaaggg gtcgagaccg agcagcaggc
     2341 gcaactgctg cggctgctgc gctgcgacca ggcgcaaggc tatcttttca gtccaccggt
     2401 acccgccgag aaattagaaa ctctcttata atctgcggcc ttagcgcatc aggccgcatg
     2461 tccctctacg ccctaggaat gaatcaccag acggcgccgc tcgcggtgcg cgagcgcgtg
     2521 gtgttccatg tcgagcgtct gcgcgaggcg ctgaccgaga tcaagggcgc gctcgcgcgc
     2581 gaagcggcga ttctttcgac ctgcaaccgc accgagctct atgtcgcggg cgagcagccg
     2641 gcgcggctcg cgcagtggct cgcccgctac caccggctcg agccgaacga gctttcgcct
     2701 tatctgtaca ccttgcccag cgaacaggct gtgcgtcatg ctttccgcgt cgcttcggga
     2761 ctcgactcga tggtgctagg cgagccgcag atcttgggcc agatgaagga cgcggcgcgc
     2821 gccgccgaat ccgcggggac gctcggcacg gtgctgcaca agctgttcca gcgctcgttc
     2881 gcggtagcca aggaggtacg ctcgacgacg cgccttggcg cgcagagcgt ctcgatggcg
     2941 gcggccgcgg tgaagctcgc cgcgcgcatt ttcccaagcc tcaaggacca gag
//
LOCUS       DASPXW010000094         2266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1795012, whole genome shotgun sequence.
ACCESSION   DASPXW010000094 DASPXW010000000
VERSION     DASPXW010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1795012"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..553
                     /locus_tag="VEL04_02220"
     CDS             146..553
                     /locus_tag="VEL04_02220"
                     /inference="COORDINATES: protein motif:HMM:NF042483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HYT47213.1"
                     /translation="MITRDQRFVASLLIVSLTSLGLPPPVYAGMVPTDAVATSAVAAT
                     GDRGRVASFLDRAEVRAQLEANGVNPAQLKARVAALTDDEAAQLAAQLDTLPAGGDGV
                     GGILFLAVFVFLVLLVTDILGFTKIFPFTRPVR"
     gene            606..>2266
                     /locus_tag="VEL04_02225"
     CDS             606..>2266
                     /locus_tag="VEL04_02225"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HYT47214.1"
                     /translation="MPNRLARETSPYLQQHAANPVDWYPWGPEALERARREDRPILLS
                     VGYSACHWCHVMAHESFEDAAVAEVMNRLFVNVKVDREERPDLDQIYQSAHQMLSQRS
                     GGWPLTMFLSPDGAPFFGGTYFPKDARHGLPAFADLCERIAALWREKRADIAAQNSEV
                     LNALARTLPQRGVARPDFSADVVRAMLDNLRANFDARLGGFGAAPKFPHPTDLELCLR
                     DGEVEMVHTTLKRMCEGGIYDQLGGGFCRYSVDAQWMIPHFEKMLYDNGPLLALLADA
                     WLVTQDELYVRCAGETAGWIMREMQSPEGGYYSSLDADSEHEEGKFYVWSREEAQRLL
                     TEKEYRAFSARFGLDQAPNFENLHWHLYASRPLEPGSETLVNDARRKLLAAREKRVRP
                     GRDEKVLVSWNALAIRGMARAGRVFGRPEWTASARRAFDFIRGRMWRDGRLLATYKDG
                     RAHLNAYLDDYAFLIAAVLELMQADFATADLEFARQLAGVLLEQFEDREGGGFFFTAR
                     DHEPLIHRPKPGHDHATPSGNAVASWALARLAALTGEERYTRAAER"
BASE COUNT          354 a          773 c          748 g          391 t
ORIGIN      
        1 gccgcgtcct tttcgaacca gcgcttgtga cgctttccat agtcgggccc ctcgccgaac
       61 cagaacagca acacgtccga gggggtgcga tagttgctca cgtaacacga tggtaagctg
      121 cccaccctaa accacggaga cttgcatgat tacgcgcgat caacgcttcg tcgccagcct
      181 gctcatcgta tcgctcacca gcctcggttt gccgccgccc gtgtacgcgg gcatggtgcc
      241 caccgatgcg gtcgctacgt ccgccgttgc agccaccggt gatcgcggcc gggtggccag
      301 cttcctggat cgggcggagg tgcgcgctca actcgaggcg aacggtgtca atccggcgca
      361 gctcaaggcg cgcgtcgccg cgcttaccga cgatgaggcg gcgcagcttg ccgcgcagct
      421 cgataccctg cccgccggcg gcgacggcgt cggcggcatc ctgttcctcg ccgtcttcgt
      481 attcctggtg ctgctggtca ccgatatcct gggttttacg aagatctttc cgttcacgcg
      541 cccggtgcgc taggcgcgaa gcgcctctca ctatcccccg ctttggcggg ggttgttttt
      601 tggacatgcc gaaccgactc gcccgcgaga catcccccta tcttcagcag cacgccgcga
      661 accccgtcga ctggtatccc tgggggcctg aggcgctcga gcgcgcgcgc cgcgaggaca
      721 ggccgatcct gctttcggtc ggctactcgg cctgccactg gtgccacgtc atggcgcacg
      781 agagcttcga ggatgctgcg gtcgccgagg tgatgaaccg cctgttcgtc aacgtgaaag
      841 tcgaccgcga agagcggccc gacctcgacc agatctacca gagcgcgcac cagatgctgt
      901 cgcagcgctc cggcggctgg ccgctcacga tgttcctttc gcccgacggc gcgccgttct
      961 tcggtggcac ctacttccca aaggacgcgc gtcacggcct gccggctttc gccgatctgt
     1021 gcgagcgcat cgcggcgctc tggcgcgaga agcgggctga catcgccgcg cagaactcgg
     1081 aagtgctgaa tgcgctcgcg cgcacgctgc cgcagcgcgg cgttgcgcgg ccggattttt
     1141 ccgccgatgt ggtgcgcgcg atgctggaca acctgcgcgc caatttcgat gcgcgtctcg
     1201 gcggcttcgg tgccgcgccg aagttcccgc atccgaccga cctcgagctg tgcctgcgcg
     1261 atggcgaggt cgagatggtg catacgacgc tgaaacgcat gtgcgagggc ggcatctatg
     1321 accagctcgg cggcggcttc tgccgctaca gcgtcgacgc gcaatggatg atcccgcatt
     1381 tcgagaaaat gctctacgac aacggcccgc tgctcgcgct gctcgccgac gcctggctcg
     1441 tcacgcagga cgagctctat gtgcgctgcg ccggcgagac cgcggggtgg atcatgcgcg
     1501 agatgcaatc tccggaaggc ggctactact cctccctcga tgcagacagc gagcacgagg
     1561 agggcaagtt ctacgtctgg agccgcgaag aggcgcagcg gctcctgacc gagaaggaat
     1621 accgggcgtt ctctgctcgc ttcggcttgg accaggcgcc gaatttcgag aacctgcact
     1681 ggcatctata cgcatcgcgc ccgctcgagc cgggcagcga gacgctcgtc aacgacgcca
     1741 ggcgaaaact gctggcggcg cgtgagaagc gggtgcgccc tggccgcgac gaaaaagtgc
     1801 tggtgtcgtg gaacgcgctc gcgatccgcg gcatggcgcg agccgggcgc gtgttcggtc
     1861 ggcccgagtg gaccgcgtcg gcgcgccgcg cgttcgattt cattcgcggg cgcatgtggc
     1921 gcgacgggcg gctgctcgcc acctacaagg atggacgcgc gcacctcaac gcctacctcg
     1981 acgactacgc ctttctcatc gccgcagtgc tggagctgat gcaggcggat ttcgcgaccg
     2041 cggacctcga gtttgcgcgc cagcttgccg gggtgctgct ggaacagttc gaggaccgcg
     2101 agggcggcgg attcttcttc acggcgcgcg accacgagcc cctcatccac cggccgaagc
     2161 ccgggcatga ccacgccacg ccgtccggaa acgcggtcgc gtcctgggcg ctcgcgcggc
     2221 tcgccgcgct caccggcgag gagcgttaca cgcgcgcggc ggagcg
//
LOCUS       DASPXW010000095         2797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_408055, whole genome shotgun sequence.
ACCESSION   DASPXW010000095 DASPXW010000000
VERSION     DASPXW010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408055"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1228)
                     /locus_tag="VEL04_02230"
     CDS             complement(<1..1228)
                     /locus_tag="VEL04_02230"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HYT47215.1"
                     /translation="MAGPKYPGFDTLALHAGQSPDPATGSRAVPIYQTTSYVFRDTEH
                     AASLFNMERAGHVYSRISNPTVAVLEERIAALEGGVGAIATASGQAALHLAIATLMGA
                     GSHIVASTALYGGSHNLLAYTLKRFGIDTTFVSPREPDSFKQAIKADTRLLFGETLGN
                     PGLDVLDVPAIAAIAHQAGIPLLVDATFTTPYLMKPFVHGADLLYHSATKFLGGHGVA
                     IAGVLVDSGRFDWEASGKFATLTAPYEGFHNMDFEEESGTRAFLLRARREGLRDFGAC
                     MAPMTAFQILQGVETLHLRMPRHVENTRKVVAFLSSHSAVEKVIYPELQNHPDNALAK
                     KLLPLGCGAVFSFEIRGGRDAGKKFIEALRVFSHLANVGDAKSLVIHPATTTHYRMSD
                     EDLKKAGITAGTVRLSI"
     gene            complement(1241..1795)
                     /locus_tag="VEL04_02235"
     CDS             complement(1241..1795)
                     /locus_tag="VEL04_02235"
                     /inference="COORDINATES: protein motif:HMM:NF042855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6806 family protein"
                     /protein_id="HYT47216.1"
                     /translation="MRHEVHLHADIPILEGVSRRQIELAFAPLLDYLDADGIADVKSL
                     EPDEPGIKYDEKELIVYICWTGETGRSFQGALEAALENVGPYCYEAVEVDLTLYLENG
                     DQESKLLFVGPTAHAIHEAQRRCMIEDVAAILARQFEKDKVGEVTTLVNDLFDRDWKE
                     KASQQKKPERAFESYTRPSRKNLH"
     gene            1856..>2797
                     /locus_tag="VEL04_02240"
     CDS             1856..>2797
                     /locus_tag="VEL04_02240"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HYT47217.1"
                     /translation="MRPRVLPDYGGGSLVNLLASIVAACGGKPLHPPLRELRIEELSA
                     ARNIVLLIIDGLGDNFLRRRGAGGELGRRRRAAITSVFPSTTASAITTSYTGCSPLEH
                     GLTGWFTYFGEVGCVAATLPFRSRGDNRSLLERGFSPERAYTAHPVFETLPLRSIVVT
                     HKEIVDSAYNQRHCRGAERRAYETVGELVSEVEAAVKSGPERKFVYAYWPEYDTVSHR
                     YGSESADALQKFMLIDEAVGKLLSRLSGTESAVIATADHGFIDAPPEQCLEPPAAISA
                     LLRFPLCGERRVAYCHVHSENEFMKRSIDWLGENAEVR"
BASE COUNT          470 a          900 c          911 g          516 t
ORIGIN      
        1 cgatcgacag gcgcacggtg cccgcggtga tgcccgcctt cttcaggtcc tcgtcgctca
       61 tgcggtagtg cgtggtggtc gccgggtgga tcaccaacga tttcgcgtcg ccgacgttgg
      121 cgaggtgcga gaagacgcgc agcgcctcga tgaatttctt tcccgcatcg cgcccgccgc
      181 ggatctcgaa cgagaacacc gcgccgcagc ccaggggcag caatttcttg gcgagcgcat
      241 tgtccgggtg attttgaagt tcgggataaa tgactttttc aactgcggaa tgcgatgaaa
      301 gaaaggcaac caccttccgc gtgttctcga catgccttgg catgcgcagg tgcagcgtct
      361 cgacgccctg caggatctgg aacgcggtca tcggcgccat gcaggcgccg aagtcgcgta
      421 atccctcgcg tcgcgcgcgc agcaggaacg cccgcgtgcc cgattcctcc tcgaaatcca
      481 tgttgtggaa gccctcgtag ggcgcggtga gggtggcgaa cttgcccgag gcctcccagt
      541 cgaagcgccc cgagtcgacc agcacgccgg cgatcgccac gccgtgcccg ccgaggaact
      601 tggtggccga gtggtagagc agatcggcgc catgcacgaa cggcttcatc aggtaaggcg
      661 tggtgaaggt cgcgtcgacc agcagcggaa tcccggcctg gtgggcgatg gcggcaatgg
      721 cggggacgtc gagcacgtcg agccccggat tgcccagcgt ttcgccgaac agcagccgtg
      781 tatcggcctt gattgcctgc ttgaagctgt ccggttcccg gggggagacg aaagtcgtgt
      841 cgatgccgaa gcgtttcagc gtataggcaa gcaggttgtg cgagccgccg tagagcgcgg
      901 tggaagccac gatgtggctg cctgcgccca tcagcgtcgc gatggcgagg tgcagcgccg
      961 cctgtccgga ggccgtcgcg atcgcgccca ctccgccctc gagcgccgcg attctttcct
     1021 ccaggacggc aaccgtggga ttcgagatgc gcgaatacac gtgcccagcg cgctccatgt
     1081 tgaagagcga cgcggcgtgc tcggtgtcgc ggaagacgta cgaggtggtc tgatagatcg
     1141 gcacggcgcg gctgccggtc gccgggtcgg gcgactggcc ggcgtgcagg gccagcgtgt
     1201 caaaaccggg atacttggga cccgccactc ggtcccgctc tcagtgcagg tttttcctgg
     1261 acggccgggt gtagctctcg aacgcgcgct cgggcttttt ctgctgcgaa gccttttcct
     1321 tccagtcgcg atcgaacagg tcgttcacca gcgtcgtgac ctcgcctacc ttgtccttct
     1381 cgaactggcg cgcgaggatc gccgcgacgt cctcgatcat gcagcggcgc tgcgcctcgt
     1441 ggatcgcgtg cgcggtaggc ccgacgaaca gcagcttcga ttcctggtcg ccgttctcca
     1501 ggtaaagcgt caggtcgacc tcgactgcct cgtagcagta gggtccgacg ttctcgagcg
     1561 cggcctcgag cgcgccctgg aagctgcgcc cggtctcgcc ggtccagcag atgtacacga
     1621 tcagctcttt ctcgtcgtac ttgatgccgg gctcgtcggg ctcgaggctc ttcacatccg
     1681 cgatgccgtc ggcgtcgaga tagtcgagca acggcgcgaa cgccagctcg atctgccgcc
     1741 gcgaaacgcc ctcgaggatc ggaatgtcgg cgtggagatg cacttcgtgc cgcatggcgc
     1801 gaattacctc ggagctcttg gaaggggagt gccgattcta cccctaaaat cgggcatgcg
     1861 accccgcgtg ttacccgact acggcggcgg cagcctggtc aatctcctcg cttcgatcgt
     1921 cgcggcctgc ggcggcaagc cgcttcaccc gccgctcagg gaattgcgca ttgaagagtt
     1981 gagcgccgcg cgaaacatcg tgcttctcat catcgacggc cttggcgaca acttcctgcg
     2041 gcgccgcggc gccggcggcg agctcggccg gcgcaggcgc gccgcgatca cctccgtctt
     2101 tccatcgacc actgcgtcgg cgatcaccac ctcgtacacc ggctgctcgc cgctcgagca
     2161 cgggctcacc ggctggttca cgtatttcgg cgaagtcggg tgcgtcgccg ccaccctgcc
     2221 tttcaggagc cgcggggaca acaggtcttt actggaaaga gggttttcgc ctgaaagggc
     2281 ctatacggca cacccggtgt tcgagacgct gccgcttcgc tcgatcgtcg tcacgcataa
     2341 ggagatcgtc gattcagcgt acaaccagcg ccattgccgc ggcgccgagc ggcgcgccta
     2401 cgagacggtc ggggagctgg tatctgaagt cgaagccgcg gtcaaatcgg ggccggaacg
     2461 aaaattcgtc tacgcttatt ggccggagta cgacacggtg tcgcaccgct acggctcgga
     2521 aagcgccgac gctctgcaga aattcatgct catcgatgaa gcagtgggca agctgctctc
     2581 gcgcctgtcg ggcaccgagt cggccgtgat cgcgaccgcc gatcacggct tcatcgacgc
     2641 cccgccggag cagtgtctcg agccgcctgc tgcgatctcc gcgctactca ggtttccgct
     2701 ctgcggcgag cgccgcgttg cctactgcca cgtccactct gaaaatgaat tcatgaaaag
     2761 aagcattgac tggctcggcg agaacgcgga ggtccga
//
LOCUS       DASPXW010000096         2666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1667822, whole genome shotgun sequence.
ACCESSION   DASPXW010000096 DASPXW010000000
VERSION     DASPXW010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1667822"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="VEL04_02245"
     CDS             complement(<1..670)
                     /locus_tag="VEL04_02245"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HYT47218.1"
                     /translation="MAKVSRKIGLAALTVLELPHHEQVSVAAQAGYSHVGLRLNPVAG
                     QPFHHTLDVNEVEKRLADTGVRVFDVEVFRLTPETKINELESIIATAARLRATELLVH
                     GADPDAIRLTESFARLCDLAAGYGLSANLEPMPWVDVSNLAKAQRVLDAAGRKNSGLL
                     VDAIHFFRAGDSPGALAKVGREVLRYMQLCDAPPERPSDMQEIIRQARSDRLFPGEGG
                     LDLRG"
     gene            complement(658..1545)
                     /locus_tag="VEL04_02250"
     CDS             complement(658..1545)
                     /locus_tag="VEL04_02250"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HYT47219.1"
                     /translation="MRKLLLGLIGAGIQRSLSPALQEEEARQHGLRLHYQLIDLDAAG
                     LDVEVLPRLIAASRVMGFAGLNITYPCKQAVIPLLDALSEEARAVGAVNTVVREGDRL
                     VGYNTDGSGWSWGFRRALPGADLSRVVLLGAGGAGSACADAVLRLGAQRLVIFDRDAP
                     RAESLTKRLNEYLHGDRASASPDLRAALHRASGLIHATPTGMAQLPGMPMPEEWLRPA
                     IWVSDIVYVPLETPLLKAARRIGCETADGGHMNVGQAVGAFKLFTGLDADPARMDAHF
                     RRLVSSPAAAAQQEETWPR"
     gene            complement(1542..1670)
                     /locus_tag="VEL04_02255"
     CDS             complement(1542..1670)
                     /locus_tag="VEL04_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47220.1"
                     /translation="MSKPKTIRRVVTGHRADGGDAVCRLGMVFIDAEEPAELAALR"
     gene            complement(1667..2287)
                     /locus_tag="VEL04_02260"
     CDS             complement(1667..2287)
                     /locus_tag="VEL04_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47221.1"
                     /translation="MLGRAAVAMWWDVASEMRAEWEDWHSSEHMPERLAIPGFLRGTR
                     WVAESGEPSYFVLYEVTHLATITGGAYLERLNNPTPWSRRMMPHHRNMVRSLCALRAG
                     WGGGLPQALATVRFSPQAAALPKLPQRKGLTGAHLLESQPMAGAPQTAEQKIRGGDGT
                     ADWVLLVGGYDVEAVKAVVTDELDAQPERFVGLYRPAYSLSSGDIA"
     gene            complement(2294..>2666)
                     /locus_tag="VEL04_02265"
     CDS             complement(2294..>2666)
                     /locus_tag="VEL04_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HYT47222.1"
                     /translation="KAYELSPFVFLLAANILLLILGCFLEGTTILLVIVPVLLPTAQA
                     LGIDPVHFGVVVVVNIMIGLVTPPYGLLLFMMVKIAEVPLKDLVRDVLPFLWVMMGAL
                     ALMTFIPEIVLFLPRLLGYKS"
BASE COUNT          465 a          917 c          867 g          417 t
ORIGIN      
        1 ggccgcggag gtcgagcccg ccctcgcccg gaaacagccg atccgagcgc gcctggcgga
       61 tgatctcctg catgtccgaa gggcgttccg gcggcgcatc acaaagctgc atgtagcgca
      121 gaacctcgcg gcccaccttc gccagcgctc ccgggctgtc tccggcacgg aagaaatgaa
      181 tggcgtcgac cagcagccct gaattctttc gtccagctgc atccagaacg cgttgcgcct
      241 tggccaagtt cgagacatcc acccacggca tcggctccag gttcgccgac agcccatagc
      301 ctgcggcgag atcgcaaagg cgcgcgaagc tctcggtcaa ccggatcgcg tcgggatcgg
      361 cgccgtgcac cagaagctcg gtggcgcgaa ggcgcgctgc cgtcgcaata atggattcaa
      421 gctcattgat ctttgtttcc ggcgtcaggc ggaacacctc gacgtcgaac acgcgcaccc
      481 cggtgtcggc aagccgtttt tcgacttcgt tgacgtcgag cgtatggtga aagggctggc
      541 ccgctacggg attcagccgc aggccgacat ggctgtagcc tgcctgcgcc gcgacgctga
      601 cctgctcgtg gtgcggcagc tccagcacgg tcagcgcggc gaggccgatt ttccggctca
      661 ccttggccat gtttcttctt gctgagcggc ggcagcgggc gacgaaacca ggcggcggaa
      721 atgcgcgtcc atccgcgccg ggtcggcgtc gaggccggtg aacagcttga aagcgccgac
      781 ggcctgacca acgttcatgt gtccgccgtc ggcggtttcg cagccgatgc gtcgcgccgc
      841 cttcaaaagc ggcgtctcaa gcggcacata cacgatatcc gatacccaga tcgcgggtcg
      901 gagccattcc tccggcatcg gcatgccggg cagttgcgcc attccggtgg gcgtcgcgtg
      961 gatcaatccg ctcgcgcggt gcaatgccgc gcgcaggtcg gggctggcgc tcgcgcggtc
     1021 gccgtggaga tattcgttca gccgctttgt cagcgactcg gcacgcggcg cgtcgcggtc
     1081 gaagatgacg aggcgttgcg cgcccaagcg cagcaccgcg tcggcgcacg ccgacccggc
     1141 gcctccggcg ccgaggagca ccacgcgcga caggtcagcc cccggaagcg cgcgcctgaa
     1201 tccccagctc cagcctgagc cgtcggtgtt gtagccgacc aggcggtcgc cttcgcgcac
     1261 cacggtgttg accgcgccga ccgcgcgcgc ctcctcggac agcgcgtcga ggagcgggat
     1321 caccgcctgc ttgcacgggt aggtgatgtt cagccccgcg aagcccatga cgcgcgacgc
     1381 tgcgatcagg cgcggcagta cttcgacatc taagcccgca gcatcgagat cgatgagctg
     1441 gtaatgcagg cgcagcccat gctggcgggc ttcctcctcc tgcagcgcgg gagagagcga
     1501 gcgctggatg ccggccccga tgaggccgag gagcagcttc ctcaccggag cgccgccagt
     1561 tccgccggct cctcggcgtc gatgaacacc atgccgagcc ggcacaccgc gtccccgccg
     1621 tcggcgcgat gaccggtgac gacacgcctg atcgtcttcg gcttgctcat gcgatgtctc
     1681 ccgaactcag cgaatacgcg ggacggtaga ggccgacgaa gcgctcgggc tgcgcgtcga
     1741 gctcgtccgt cacaaccgcc ttcacggcct cgacatcgta gccgccgacg agcagcaccc
     1801 agtctgcggt tccgtccccg ccgcggattt tctgctcggc agtctgcggc gcgccggcca
     1861 tcggctgcga ctcgagcaga tgcgcgccgg tcaggccctt gcgctgcggc agcttaggca
     1921 gcgcggccgc ttgcggcgag aaccggaccg tcgccagcgc ctgcggcaac ccgccgcccc
     1981 agcctgcccg aagcgcgcac aggctgcgca ccatgttgcg atggtggggc atcatccttc
     2041 gcgaccacgg cgtgggattg ttcagtcgct cgagatatgc cccgccggtg atcgtagcca
     2101 agtgtgttac ttcataaaga acaaaatagg agggctcacc tgactcggcg acccagcgcg
     2161 tgccgcgcag gaatcccggt atcgcgagcc gttcgggcat gtgctcgctc gaatgccagt
     2221 cctcccactc ggcccgcatc tcagacgcta cgtcccacca catggcgact gcagcccggc
     2281 ccagcatgcc gacttagctc ttgtagccca ggagccgggg caggaagagc acgatctccg
     2341 gtatgaaggt catgagcgcg agcgcgccca tcatcaccca gaggaacggc agcacgtcgc
     2401 gcaccaggtc cttgagcggc acctcggcga tcttcaccat catgaaaagc agcaggccgt
     2461 agggcggcgt caccaggccg atcatgatgt tgaccaccac cacgacgccg aaatgcaccg
     2521 gatcgatgcc gagcgcctgc gcggtcggca gcaggaccgg cacgatcacc agcaggatgg
     2581 tggtgccttc gaggaaacag ccgaggatca gcaacaggat gttcgccgcg aggaggaaaa
     2641 cgaagggcga gagctcgtac gccttc
//
LOCUS       DASPXW010000097         3796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_660495, whole genome shotgun sequence.
ACCESSION   DASPXW010000097 DASPXW010000000
VERSION     DASPXW010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660495"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /gene="argB"
                     /locus_tag="VEL04_02270"
     CDS             complement(<1..817)
                     /gene="argB"
                     /locus_tag="VEL04_02270"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HYT47223.1"
                     /translation="MNAPFNHSPQAAEKARILAEALPYIQRFHGRTIVVKFGGNAMTD
                     DELKAGFARDVVLLKLVGMNPVVVHGGGPQIEEMLARLGKKGEFVQGMRVTDRETMDV
                     VEMVLGGQVNKEVVELVNQAGGKAVGLTGQDGAFIRARKMLLPSKDNGTIDIGHVGEI
                     DSIDPSVIEALESAGFIPVVAPIGTGADGTTYNINSDLVAGKLAEILRAEKLVVLTNT
                     PGVLDKNGKLLTGLTPRKIDDLVTKGVISGGMLPKIGSALDAARSGVKSVHIID"
     gene            complement(845..1273)
                     /locus_tag="VEL04_02275"
     CDS             complement(845..1273)
                     /locus_tag="VEL04_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HYT47224.1"
                     /translation="MNTITRYNPFNELVDDLFNGFLVRPVAYEGLGDVLPRVKVDVAE
                     KNGAYKVTAELPGVKKEDIQVSIDGAQVTLSAEVRQEKEASPEERVLHTERVFGKVSR
                     SFTLPQELDEAKAEAKFRDGVLELTLPKKAEAARKQISIQ"
     gene            1371..1910
                     /locus_tag="VEL04_02280"
     CDS             1371..1910
                     /locus_tag="VEL04_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYT47225.1"
                     /translation="MSDFRSRVEGERAYLLRYASLQLRDRHSAEDVVQEALLAALAGE
                     AGFAGRSNLRTWLTGILKHKIVDAIRRSSREASVAPDGDEFESLFDERGHWVDAPAAW
                     TDPGASLEQKQFFAALEDCLARLPVKTAQAFTLREHLGLDTGEICKELSVTATHCWVL
                     LYRARMALRECLEASWFKK"
     gene            1910..2101
                     /locus_tag="VEL04_02285"
     CDS             1910..2101
                     /locus_tag="VEL04_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HYT47226.1"
                     /translation="MLSCKEVTRLVSQGLDRRLGFGERLQLHIHLAICDGCANFRRQM
                     LFLRKAMARLADLESPAES"
     gene            2358..2666
                     /locus_tag="VEL04_02290"
     CDS             2358..2666
                     /locus_tag="VEL04_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47227.1"
                     /translation="MALAVVLVALLPVAPASASQHAANTTVSQSPCGSEGAACAEVCK
                     PDSAPAIEHLQAPMSPPASTEVDARDKILPDPAHFIASIASLAQAGPPVYLRYHCFLL
                     "
     gene            2770..>3796
                     /locus_tag="VEL04_02295"
     CDS             2770..>3796
                     /locus_tag="VEL04_02295"
                     /inference="COORDINATES: protein motif:HMM:NF012612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HYT47228.1"
                     /translation="MNRRDFLRNAAALGGLALLPPVAAAVPRVIQAKLATASLVGAQY
                     PATRVWAYAGTVPGPELRFRQGERLRVELENLLPEDTTVHWHGVRLPNAMDGVPHVTQ
                     PPIAANGGRFLYEFELRDAGTYWYHPHLGSSGQVGRGLYAPLIVEEQEPPAVDRDVTW
                     MLGDWRLDRRARIVEDFGNFMDASHDGRIGNTVAVNGAIREAFQLRGGERIRLRLVNA
                     ANARIFALEFRGHDPIVIALDGHPVEPHRPDGGRVVLGPAMRADVLLDATGAPGGKYA
                     VLDDFYPRRAYRVLDLRYAKERLRSGRAGAPLPKLAANALPEPDLARAERHRIEFGGG
                     MMGGMMGM"
BASE COUNT          578 a         1291 c         1263 g          664 t
ORIGIN      
        1 cgtcgataat gtgcacgctc ttcacgccgc tgcgtgcggc gtcgagcgcc gagccgatct
       61 tcggcagcat gccgcccgag atcacgccct tggtcaccag gtcgtcgatc ttgcgcggcg
      121 tgaggccggt gaggagcttg ccgttcttgt cgagcacgcc gggagtattg gtgagcacca
      181 cgagcttctc ggcgcgcagg atctcggcca gcttgccggc gacgaggtcg gagttgatgt
      241 tgtaggtggt gccgtcggcg ccggtgccga tcggcgccac caccggaatg aaacccgccg
      301 actcgagggc ctcgatcacc gacggatcga tcgagtcgat ctcgccgacg tggccgatgt
      361 cgatggtgcc gttgtccttg gagggcagca gcatcttgcg ggcgcggatg aaggcgccgt
      421 cctgccccgt aaggcccacc gccttcccgc cggcttgatt aacgagctcg accacttcct
      481 tgttgacctg gccgccgagc accatctcga ccacgtccat ggtctcgcgg tcggtgacgc
      541 gcatgccctg cacgaactcg cccttcttgc ccaggcgcgc gagcatctcc tcgatctgcg
      601 ggccgccgcc gtgcaccacc accgggttca tgccgaccag cttcaggagc accacgtcgc
      661 gcgcgaaccc ggccttcagc tcgtcgtcgg tcatggcgtt gccgccgaac ttgacgacga
      721 tggtcctgcc gtggaagcgc tggatgtaag gcagcgcctc ggcgaggatg cgcgcttttt
      781 cggcggcctg gggactgtgg ttgaaggggg cgttcatgcg cgccccccga tactacagaa
      841 aaacttactg gatcgagatc tgcttgcgcg ccgcctcggc cttcttgggc agcgtcagct
      901 ccagcacgcc gtcgcggaac ttggcttcgg ctttcgcctc gtcgagctcc tgcggcagcg
      961 taaagctgcg actcaccttg ccgaacacgc gctcggtgtg gagcacgcgc tcctccgggg
     1021 aagcttcctt ctcctgcctc acctcggccg acagggtgac ctgcgcgccg tcgatcgaga
     1081 cctggatgtc ttccttcttc acccccggca gctccgcggt gaccttgtag gcgccgttct
     1141 tctcggcgac gtccactttc acgcggggca gcacatcgcc cagaccttca taggcgaccg
     1201 ggcgcacgag gaaaccgttg aacaggtcgt cgaccagctc attgaatggg ttatagcgag
     1261 taatggtgtt catagtcctc tcctggctga atgactgggg gctctcgccc catgcaagcg
     1321 atatgggggt tgcttcgaca gcttcaagcc cccggtaaaa tccccgttcc atgagcgact
     1381 tccgttcccg ggtggagggc gagcgggcct acctgctgcg ctacgccagc ctgcagctac
     1441 gggaccgcca cagcgccgag gacgtggtgc aggaagccct gctcgcggcg ctcgccgggg
     1501 aggcgggatt cgccgggcgc tcgaacctgc gcacctggct caccggcatc ctcaagcaca
     1561 agatcgtcga cgcgatccgg cgctcgagcc gggaagccag cgtcgctccg gatggagatg
     1621 aattcgaatc tttgttcgac gagcgcggcc attgggtcga tgcgccggcc gcctggaccg
     1681 atcccggcgc ctcgctcgag cagaagcagt tcttcgccgc gctcgaggac tgcctggcgc
     1741 gccttccggt gaagactgcg caggcgttca cgctgcgcga gcacctcggg ctcgacaccg
     1801 gggaaatctg taaggaactc agcgtcacgg cgacgcattg ctgggtgctc ctctaccgcg
     1861 cgcgcatggc gctgcgcgag tgcctcgaag ccagctggtt caagaaatga tgctttcgtg
     1921 caaggaagtg actcgcctgg tatcgcaggg cctcgaccgc cgcctcggct tcggcgagcg
     1981 gctgcagctt cacatacacc tcgcgatctg cgacggctgc gcgaatttca ggcgacagat
     2041 gctcttcctg agaaaggcca tggcgcgtct tgccgacctc gaatcccccg cagagtcctg
     2101 acgcgcgctg cgccgcctgc ggcgcgccgt ttccttgcgg caaggacgaa gccgagtgct
     2161 ggtgcgcggc gctgccgccg ctcaccccca ctacggggcg cggctgcctg tgcaggaagt
     2221 gcctggaaga ggagctgaaa gccgcttctc agaggacgta gggatttggt cgtctccaag
     2281 gcgctaccgc tgcggcgggc cgggttatga ctgaacttgc gtgccggggt agactccaaa
     2341 tcccgatgcg tatcgtaatg gccctcgccg tagtgctcgt ggcgctgctc cccgtcgcac
     2401 cggcttccgc ttcgcaacac gctgccaaca ctactgtttc gcaaagtcct tgtggcagcg
     2461 aaggcgcggc ttgcgccgaa gtctgcaagc cggattcggc gcccgccatc gagcatctgc
     2521 aggccccaat gtccccgccc gcctcgaccg aggtggacgc gcgcgacaag atcttgccgg
     2581 acccggcaca cttcatcgcg tcgattgcgt cgctcgcaca agccgggccg ccggtctatc
     2641 tgcgctacca ctgcttcctc ctctgagcgc caccagccgc tgaacgaatg cggcgcgtgc
     2701 ggcttgcgcg cgccggctgg ccgcgtcgcg gccatgcagg gagagcagag gatcatggat
     2761 cgggctggga tgaatcgcag ggatttcctt cgcaacgctg ccgcgctcgg cgggctcgcg
     2821 ctgctgccgc cggtcgcggc ggcggtgccg cgcgtaatcc aagcgaagct tgcgaccgca
     2881 tcgctcgtcg gagcgcagta cccggcgacc cgcgtctggg cgtacgcggg aaccgtgccc
     2941 ggtcccgagc tgcgcttcag gcagggcgag cggctgcgtg tcgagctgga aaatcttctg
     3001 ccggaggaca ctaccgtgca ctggcacggc gtgcggctgc cgaacgcgat ggacggcgtg
     3061 ccgcacgtga cgcagccgcc gatcgcggcg aacggcggcc gcttcctgta cgagttcgag
     3121 ctgcgcgacg ccggcaccta ctggtaccac ccgcatctcg gcagctcggg ccaggtcgga
     3181 cgcgggctgt acgcgccgct catcgtcgag gaacaggagc cgccggcggt cgaccgtgac
     3241 gtcacttgga tgcttggcga ctggcgcctc gatcgccggg cgcgcatcgt cgaggacttc
     3301 ggcaacttca tggatgcgag ccacgatggc cgcatcggca acacggtcgc cgtaaacggc
     3361 gcgattcggg aggcgttcca gctgcgcggc ggcgagcgca tccggctgcg gctcgtcaac
     3421 gccgcgaacg ctcgcatctt cgcgctcgag tttcgcggcc acgatccgat tgttatcgcg
     3481 ctcgacggcc atccggtgga gccgcacagg ccggacggcg ggcgcgtcgt gctcggcccg
     3541 gcgatgcgcg ccgatgtgct gctcgacgcg actggcgcgc caggcggcaa gtatgcggtg
     3601 ctcgacgatt tctatccgcg ccgcgcctat cgcgtgctcg acctgcgcta cgcgaaggag
     3661 cggcttcgca gcggacgcgc cggcgcgccg ctgccaaagc tcgccgccaa cgcgcttccc
     3721 gagccggacc tggcgcgcgc cgagcgccac cgcatcgagt tcggcggcgg aatgatgggc
     3781 ggcatgatgg gcatgg
//
LOCUS       DASPXW010000098         5393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1039638, whole genome shotgun sequence.
ACCESSION   DASPXW010000098 DASPXW010000000
VERSION     DASPXW010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5393
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039638"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..513
                     /locus_tag="VEL04_02300"
     CDS             244..513
                     /locus_tag="VEL04_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47229.1"
                     /translation="MASDLTFTGERFLPDCSGEIAYEHWHRYPFARRFAVGRRGLDAA
                     CGEGYGTALLETIEHLHAAGTRSLPARPEAVARRASGTLVNGRRT"
     gene            906..3896
                     /locus_tag="VEL04_02305"
     CDS             906..3896
                     /locus_tag="VEL04_02305"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYT47230.1"
                     /translation="MLANGAAYRRWDNLAITLVAAPPSAARSAFLLELGGSRISEFPP
                     LLLALTAEEMIASGAFEQAREVLARTELLAQTIYDPEVLRRLALAEASSGSSKSWAER
                     YALRCVELVASTPQVAWPLRTAGEPLRIAYLITPGTPIVIGGVSVEPGAYLRAVVAAH
                     PRERFAARVYVVGDAGMESLAELLPATVPVEKLVVPVELAVARRVAESDPDALIDLTG
                     MRAPLGLLLAWRPARMLWTYPRLAGAHVAPLPMHALPALAASDEPALAQHRLALERAL
                     GEACAGELALSAARTRTAADLATAWRSAVAAHETGEVDAAIAGYREVLAEQPDYAPAQ
                     HLLGVLLRDRGQRREAEQAFEAALAAAPAYPEARIALANLYRENGRARTAGKLCVEGI
                     ALTPNEVSLWRALGQARFAQGRGRAAWKAFKNALKLAPADGETHYNEGVALQMLHRRG
                     LALRAYQRALALSPELIAADFNIGVIFREQGRAEAAISAFAHVLAHDPRHVAAHKALA
                     ETLLAAGRIDDWLKTFERFEAACPDALPLAVMALEACQYRGDFAALDRYLDRLERDEF
                     RAAGDTELADCLEELLYLLLFFDVDPEWHFSLYKRYDAVAGRVYGRPSPLPEERRPGK
                     IRIGYLSGDLRDHVMGKMMWQAIERHDRERFEIFFYSLSTASDEWTARYRGLSEHFEI
                     IAGLSEREAAKRIAADDLDLLVDLATNTAGAKPGILALKPARVQLTHVASAGVVGLSA
                     VDFKLTDAYADLPENQQFQLETLLPMQGCVYPYRHVAPAPEHPFHRDRIGIAADKIVI
                     GAFVNQLKLSRRCLALWCEILERVPNAVLAVSPRSPEHRAIYGRLFSAAGIAHSRVCV
                     VPQGRDDAENRARYNIVDFVLDPMPFGSANGTLEPLDMGVPVVTLAGRRHGERCAYSI
                     LGNLGVTQTVAHSGSEYVEIAVRLATDAPFRTEVSAAIRNALEHSALTDMDAHTRHLE
                     RAYLEALEQRYPAALRTGRDGTDG"
     gene            3889..>5393
                     /locus_tag="VEL04_02310"
     CDS             3889..>5393
                     /locus_tag="VEL04_02310"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYT47231.1"
                     /translation="MADIAAASSGIERARMRLAEGDLATARSEAEAIIGSAAGNEERC
                     AAYLVLAVCCDRAGDAAAALEHTRAAVDSAPGDPVAHYAHAEQQEASGDKAGAIASLR
                     RAIEIEPRFARALRYLGILLGEDGDAEGAIAALERALAFEPNHSRAWNNLGNAQRTLG
                     RLAEAERSFARALALQPDYPLAAANLGEVLRDQGEVERAEATLRAALARQAGGPPFRP
                     QVVLLAGLLRERGALDEAESLYRQAIDLAPRESGGQWASLAWVLSERGETAAAREAYA
                     TARTVDASDLRNLLGKHLTLPMIYADAGHLASERAAFAAGLTALQHELPAAASALAEE
                     KLLDGFRWTNFFLAYQGQDDRVLQAAYASLVAQAIDAGAPRWRPRIEARRTTGRRLRV
                     GFASAFFHVGTCGRYFRSWITDLDRDRFEVFVYHLYPGMDEIASAIVRRADCFRGFAG
                     MRARPSVVAPTIRADELDVLVYPELGMDACSFALAALRLAPRQYAGWGHPVT"
BASE COUNT          833 a         1809 c         1844 g          907 t
ORIGIN      
        1 cgcttggcgg gtatcgattc gtcgcgattg aaaaagattt tcccgggacg ggttgaacgc
       61 ttcgaagtcg gcagaacgca ccccgaagtg ctcgatcagc acaatcgtgc attcgccaag
      121 gcgttggaac gctgtctgag cgtgatccac gaatacgcgg acgacccgcg gcttccccat
      181 ctcgcgcagc gtgcccgccg gtaggggcga atttcattcg gccgcggcgc tactcggctc
      241 tcgatggcct ccgatctgac cttcacggga gaacgcttcc tgcctgactg cagcggcgaa
      301 atcgcctatg agcactggca ccgctacccc ttcgcgcggc gcttcgccgt cggccggcgc
      361 ggcctggatg cggcctgcgg cgaaggctac ggcaccgcgc tcctcgagac catcgagcac
      421 ctgcacgccg ccgggacgcg ctctctaccg gctcgccccg aggccgtcgc tcgacgcgct
      481 tccggaacgc ttgtgaacgg aaggcgcaca tgatccgcgc ggattacgca aagtggctgg
      541 cgcgtggcca cgagcacgag caggcggggc ggtcgatcga cgccatcgtg tgctatcggc
      601 gtgcgctcaa ttccaaccgg cactcggtgg aggcgcagta tcgcctcggt caactgctgc
      661 gcggcctcgg ccgtcataag gaagcgcgcg cggcgtggct ggcgggcctc gcgcttagcc
      721 caggggacga gcgcttgctc ctgggtgtgg cggccacagc tcggcgggca ggggctcatt
      781 ccgaggcgat cgacgcctac gagcggatcc tggcgggcga tcccgagcac gttggcgcgc
      841 gcatgggagt cgcattgtct cgagtcgcgc agggcgacga agcagcctat gcagacttga
      901 gcacggtgct cgccaatggc gcggcgtacc ggcgctggga caatctggca attaccctgg
      961 tggccgcgcc gccgtccgcc gcgcgcagcg cattcttgct cgagcttggt gggtcgcgga
     1021 taagtgagtt cccgcctctg ctcctcgccc tgaccgcaga agagatgatc gcctccggcg
     1081 cattcgagca ggcgcgcgag gtgctggcgc gaaccgaact acttgcgcag acgatctatg
     1141 atccggaggt tttgcgccgg ctggcgcttg ccgaggcttc gagtggatcc tccaagtcct
     1201 gggcagagcg ttacgcgctc cgctgcgtgg agctagtcgc gtccactccg caggtggcct
     1261 ggccgcttcg taccgcgggc gagccgctcc ggatcgcgta tctgatcacg ccgggcacgc
     1321 ctatcgtcat cgggggtgtc tccgtcgagc ccggagcgta tctgcgagca gtcgttgcag
     1381 cgcatcctcg ggagcgcttc gcggccaggg tatacgttgt cggcgacgcc gggatggagt
     1441 ccttggcaga actactgcca gcgaccgtcc ccgtcgaaaa actggtcgta cccgtagagc
     1501 tggcggtggc acgcagggtg gcagagtccg acccggacgc gttgatcgat cttaccggca
     1561 tgagggcgcc gttgggattg ttgcttgcgt ggcgtcccgc ccgaatgcta tggacctatc
     1621 ccaggctcgc gggtgcacac gtcgctccgc tccctatgca tgcgttgccc gcgctcgcgg
     1681 ctagcgacga gccggcgctg gcgcagcatc ggctggcgct ggaacgagcg ctcggcgagg
     1741 cgtgcgcggg agagttggcg ctttcggcag cgcgcacacg tactgccgcg gatctcgcca
     1801 ccgcttggcg ctcggcggtt gccgcgcatg agacgggcga ggtggacgcg gcgatcgccg
     1861 gctatcgcga ggtgctggcg gaacagcccg actatgcgcc agcccagcat ctgctcggcg
     1921 tgcttctgcg cgaccgcggg caacgccgcg aggcggagca agcattcgaa gccgcgctcg
     1981 ccgcagcgcc ggcctatcca gaagcgcgca ttgcgctggc caacctgtat cgcgagaatg
     2041 gacgggcgcg tacggccggc aagctctgcg tcgaaggaat cgcgctcacg ccgaatgagg
     2101 tatcgctgtg gcgcgcgctc ggacaggcgc ggtttgcgca gggccgcgga cgcgcggctt
     2161 ggaaggcatt caagaacgcg ctcaagctgg cgccggcaga cggcgagacg cactacaacg
     2221 agggcgtggc gctccagatg ctgcatcggc gcggtctcgc gttgcgcgcc tatcagcgag
     2281 cgctggcgct ctcgcctgag ctgatcgcgg ccgacttcaa catcggcgtg atctttcgcg
     2341 agcagggtcg agcagaggct gcgatcagcg cgttcgcgca cgttcttgca cacgaccctc
     2401 ggcacgtcgc cgcgcacaag gcgctcgccg agacgctgct cgccgccgga cggatcgacg
     2461 actggttgaa gacattcgag cgcttcgagg ccgcgtgtcc cgacgcactc ccgctcgccg
     2521 taatggcgct cgaggcctgt cagtatcgag gcgatttcgc tgcgctcgat cgctatctcg
     2581 accgcctgga gcgagacgaa ttcagggcgg cgggcgacac cgaactggcc gactgcctcg
     2641 aagagttgct ctatctgctg ctgttcttcg acgtcgatcc cgagtggcat ttctcgctgt
     2701 acaagcgtta cgatgccgtg gcggggcgcg tctacggtcg cccgtcgccg ctgccggagg
     2761 aacgccggcc gggaaagatc cgcatcggct acctctcggg ggacttgcgt gaccatgtca
     2821 tgggcaagat gatgtggcag gccatcgagc gccacgaccg ggagcgcttc gagattttct
     2881 tctactcgct ctccaccgcg agtgacgaat ggactgcgcg ctatcgcggg ctttccgagc
     2941 atttcgagat catcgcaggg ctctcggaaa gagaggcagc caagcgcatc gctgccgacg
     3001 atctcgatct gctggtcgat ctggctacca acacggcggg cgcgaagccg ggcatcctgg
     3061 cgttgaagcc tgcgcgcgtg caactcacgc atgtcgcgag cgcgggggta gtcggtctct
     3121 ccgctgtcga cttcaagctg accgacgcct atgccgactt gcccgagaat cagcagttcc
     3181 agctcgagac gctgctgccg atgcagggct gcgtctatcc ctatcggcat gtcgcgcctg
     3241 cgcccgagca cccgtttcac cgtgaccgaa ttggcatcgc tgcggacaag atcgtgatcg
     3301 gcgccttcgt caatcagctc aagctctcgc ggcgttgcct cgcgctgtgg tgcgagatcc
     3361 tggagcgagt tcccaacgct gtcctcgccg tgtccccgcg ctcccccgag caccgtgcga
     3421 tctacggacg gctcttctcc gcggccggca tcgcgcattc gcgcgtgtgc gtggtgccgc
     3481 aggggcgtga cgacgcggag aatcgggctc gctacaacat cgtcgacttc gtgctcgatc
     3541 cgatgccctt cggcagcgcc aacggcacat tggagccgct cgacatgggc gttcccgtcg
     3601 tcacgcttgc cggacgcagg cacggcgaac gctgcgcgta cagtattctc ggcaaccttg
     3661 gcgtgacgca gactgtcgcc cacagcggga gcgagtacgt cgagatcgcg gtccggctcg
     3721 ccaccgacgc gccgttcaga accgaggtca gcgccgccat ccgcaacgcc ctcgagcact
     3781 cggcgttgac cgacatggat gcgcacacgc ggcacctaga gcgggcttac ctggaggcgc
     3841 tcgaacaacg ctatcccgcc gcgcttcgca ccgggcgcga tgggactgat ggctgacatc
     3901 gcagcggcgt cctccggcat cgagcgtgcg cggatgcggc tcgccgaagg cgaccttgcc
     3961 acagcgcgca gcgaagccga ggcgatcatc ggcagcgctg ccggcaacga ggagcgttgc
     4021 gcggcgtatc tcgtcctcgc ggtttgctgc gacagggctg gtgatgctgc cgcagcgctc
     4081 gagcacactc gcgcggccgt agacagcgcg ccgggagatc cggtcgcgca ctacgctcac
     4141 gccgagcagc aagaagcatc cggcgacaag gctggcgcaa tcgcgagctt gcgccgcgcg
     4201 atcgagatcg agccgcgctt tgcgcgggcg cttcgctacc tcggcattct cctcggggag
     4261 gacggcgacg ccgaaggggc gatcgcagcc ctcgaacgcg cgctcgcctt cgaacccaat
     4321 cactcgcgcg cctggaacaa tctgggcaat gcgcagagaa ccctcggtcg gctcgcggaa
     4381 gcggagcgct cgttcgcccg cgcgctcgct ttgcagccgg actatccgct cgccgctgcc
     4441 aacctcggtg aggttctgcg cgaccagggc gaagtcgaac gcgcggaggc cacgctgcgc
     4501 gcggcgctcg cgcgacaggc gggcggtccg cctttccggc cccaggtcgt gctgctcgcg
     4561 gggctcttgc gcgagcgcgg cgcgctcgac gaagcggaga gcttgtatcg gcaggcgatc
     4621 gacctcgcgc cgcgcgaatc gggcgggcaa tgggcgagcc tcgcctgggt cttgagcgag
     4681 cgcggggaga cggctgcggc gcgcgaggcc tatgcaaccg cgcggacggt cgatgcaagc
     4741 gacctgcgga atctgctggg caagcacttg acgctaccga tgatctacgc cgacgcgggc
     4801 cacctcgcct cggagcgcgc ggctttcgcg gccgggctga ccgcgcttca acacgagcta
     4861 cccgcggccg catcggcgct cgccgaggaa aaactcctcg acggctttcg ctggaccaac
     4921 ttcttcctcg cctatcaggg gcaggacgac cgcgtgctgc aggcggccta cgcgagtctg
     4981 gtggcgcagg cgatcgacgc aggcgcgccg agatggagac cgcggatcga agcgcgacgc
     5041 accaccggac ggcggctgcg cgtcggcttc gcctccgcgt tttttcacgt cggcacctgc
     5101 ggacgctatt tcaggagctg gatcaccgac ctcgatcgcg atcgcttcga ggtcttcgtc
     5161 taccatctct atccgggcat ggatgagatc gcgagcgcga tcgtgcggcg cgccgattgc
     5221 tttcgcggct tcgccggtat gcgcgcgcgg ccatcggtgg tcgctccgac gatccgcgcg
     5281 gacgagctcg atgttctcgt ctaccccgag ctgggcatgg acgcctgcag ctttgcgctc
     5341 gcggcgctgc ggctcgcgcc gcggcagtac gcgggctggg gtcaccccgt gac
//
LOCUS       DASPXW010000099         2668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1039936, whole genome shotgun sequence.
ACCESSION   DASPXW010000099 DASPXW010000000
VERSION     DASPXW010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039936"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="VEL04_02315"
     CDS             <1..963
                     /locus_tag="VEL04_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYT47232.1"
                     /translation="THMLNQAWLGLLKKHGGAYSVKKVRGGQTGIHRDGAHFMTLTPG
                     MFDYEARIRAMDAAGVDIAIVTLTSPNVFWGSARTSLQAAKLMNDDMAAQQKRYPERI
                     RFMCSLPWQHAKLAVAELKRACDQLGAVGVMVLANIGGASLTDRIFSSIWKEIDRRAL
                     PVLVHPTAPPGTQQLDVAKYNLIASVGFMFDTSLAVSRMIFDGFFDRHPNLKLIAAHG
                     GATLPYIAGRLDICFENMPACREKISQRPSSYLKKIYYDSVVFQQESLELVLRVGGED
                     KVLYGSDYPHNIGDMKGCLARVDALPAAQRDAVRGGNAQRIFRL"
     gene            complement(967..1914)
                     /locus_tag="VEL04_02320"
     CDS             complement(967..1914)
                     /locus_tag="VEL04_02320"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYT47233.1"
                     /translation="MTRFLLFTFGIIFCFTASAQFPSKPVRIIVPYTPGGGTDIISRQ
                     LAVKMQESWGQQVLVENRPGANGIIGTEVMVKSPPDGHTVAMVVGAHVVNAVLQSKMP
                     FDPIADTTAVSLVATSPWVIGIYPGVPAQNLREFIAYAKANPGKLRFGSSESSSRLAG
                     EQFKQVAGVDLLHVPYKGGAQIVQDMLGGHVEVGFTSVLTFLAHHKSGKLRILAVGGK
                     TRAGAMPDVPTAAEAGLPGYETYVWYGMYAPKATPRGVVGRIQQEIARIVRLPDSGER
                     LAALGADAVASTPEEFAAFTRAEYEKFAKLVKASGIQPE"
     gene            complement(1911..>2668)
                     /locus_tag="VEL04_02325"
     CDS             complement(1911..>2668)
                     /locus_tag="VEL04_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYT47234.1"
                     /translation="LQAIRMRARTDGGDYILNGTKTWISNGIQGSCFAVLAKTDPQAE
                     PRHRGMTMFLCEKGPGFTAGRKLEKLGYKGIDSAELVFQDYRVPAANRIGEEGRGFQQ
                     AVGGLELGRINVAARGVGIAQAALAEAVRYSQQRETFGKPICEHQAIQLKLGEMATRT
                     EAARLLVRQAAEIYDRGERCDMEAGMAKYFATEAAFENAHEAVRIHGGYGYSKEFMVE
                     RLYRDAPLLCIGEGTNEMQRIIIARELIRRNKA"
BASE COUNT          427 a          904 c          868 g          469 t
ORIGIN      
        1 acgcacatgc tgaaccaggc ctggctcggc ctcctgaaaa agcatggcgg cgcttacagc
       61 gtgaagaagg tgcgcggcgg gcagaccggc atccaccgcg acggcgcgca cttcatgacg
      121 ctcacgccgg ggatgttcga ctacgaggcg cgcatccgcg ccatggacgc ggcgggcgtc
      181 gacatcgcca tcgtcacgct caccagcccc aacgtgttct ggggctcggc gcgaaccagc
      241 ttgcaagcag caaaattgat gaatgatgat atggcggcac agcagaaacg ctacccggag
      301 cgcattcgct tcatgtgctc gctgccgtgg cagcacgcca agctcgcggt agcggaactg
      361 aagcgcgcct gcgaccagct cggcgcggtc ggcgtgatgg tgctggcgaa tatcggcggc
      421 gcctcgttga ccgataggat tttcagttca atttggaaag aaatcgaccg ccgcgcgctg
      481 ccggtgctgg tgcacccgac cgcgccgccg ggcacgcagc agctcgacgt cgcgaagtac
      541 aacctgatcg cctcggtcgg cttcatgttc gacacctcgc tcgccgtgag ccgcatgatc
      601 ttcgacggct tcttcgaccg tcatccgaac ctgaagctca ttgccgcgca cggcggcgcc
      661 acgctgccgt acatcgcggg acgcctcgac atctgcttcg aaaacatgcc cgcgtgccgc
      721 gagaagattt cccagcggcc cagcagttat ttgaagaaga tttattacga ctcggtggtc
      781 ttccagcagg aatcgctcga gctcgtcctt cgtgtagggg gtgaagataa agtcctttac
      841 ggctcggact atccgcacaa catcggcgac atgaagggct gcctcgcgcg cgtggatgcc
      901 ctgccggcgg cgcagcgcga cgcggtgcgc ggcggcaacg cgcagcggat cttccggctc
      961 tgagcgctat tccggctgga tgccagaggc cttgaccagc ttcgcgaact tctcgtactc
     1021 ggcgcgcgtg aatgcggcga attcctccgg cgtgctcgcg actgcatcgg cgccgagcgc
     1081 cgccagccgc tcgcccgagt cggggaggcg aacgatgcgc gcgatctcct gctggatgcg
     1141 cccgaccacg ccgcgcggcg tcgccttcgg cgcgtacatc ccataccaca cgtaggtctc
     1201 gtagccgggc aggccggcct cggccgcggt cggcacgtcc ggcatcgcgc cggcgcgcgt
     1261 cttgcccccg acggcgagga tgcgcagctt cccggacttg tggtgggcga ggaaggtcag
     1321 cacgctggtg aagcccacct cgacgtggcc gccgagcatg tcctgcacga tctgcgcgcc
     1381 gcccttataa ggcacgtgca ggaggtcgac gcccgccacc tgcttgaact gctcgcccgc
     1441 cagccgcgac gaggattcgg agctgccgaa gcgcagcttc cccgggtttg ccttggcgta
     1501 ggcgatgaac tcgcgcaggt tctgcgccgg taccccggga tagatgccga tcacccaggg
     1561 ggaagtcgcg accaggctca ccgcggtggt atcggcgatc gggtcgaagg gcatcttcga
     1621 ctgcagcacc gcgttcacca cgtgcgcccc gaccaccatc gccacggtgt ggccgtccgg
     1681 cggcgacttc accatcacct cggtgccgat gatgccgttg gcgccgggcc ggttctcgac
     1741 cagcacctgc tggccccagc tttcctgcat cttgaccgcg agctgccggc tgatgatgtc
     1801 ggtgccgccg ccgggggtgt acggcacgat gatgcggacc ggtttggacg ggaactgcgc
     1861 gctcgcggtg aaacaaaaaa tgattccgaa tgtgaaaagc aaaaagcgcg tcatgccttg
     1921 ttcctccgga tgagctccct ggcgatgatg atgcgctgca tctcgttggt gccctcgccg
     1981 atgcagagca gcggcgcgtc gcgatacagg cgctcgacca tgaattcctt cgagtagccg
     2041 tagccgccgt ggatgcgcac tgcctcgtgc gcgttctcga acgccgcttc ggtggcgaag
     2101 tacttcgcca tgccggcctc catgtcgcag cgctcgcccc ggtcgtagat ctcggccgcc
     2161 tgcctgacca gcagccgcgc cgcctcggtg cgcgtcgcca tctcgccgag cttgagctgg
     2221 atcgcctggt gctcgcagat cggcttgccg aaggtctcgc gctgctgcga gtacctgact
     2281 gcctcggcga gcgccgcctg cgcgatgccg acgccgcgcg ccgcgacgtt gatccgcccg
     2341 agctcgaggc cgccgaccgc ctgctggaag ccgcggccct cctcgccgat ccggttcgcg
     2401 gccggcacgc ggtaatcctg gaacaccagc tcggcggaat cgatgccctt gtagccgagc
     2461 ttctcgagct tgcgtccggc ggtgaagccc gggcccttct cgcacaggaa catcgtcatg
     2521 ccgcgatggc gtggctcggc ttgcgggtcg gtcttggcga gcacggcgaa gcacgagccc
     2581 tggatgccat tcgagatcca ggtctttgtt ccgttgagga tgtagtcgcc gccgtccgtt
     2641 cgcgcgcgca tccggatcgc ctgcaggt
//
LOCUS       DASPXW010000100         6045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1040180, whole genome shotgun sequence.
ACCESSION   DASPXW010000100 DASPXW010000000
VERSION     DASPXW010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6045
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040180"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VEL04_02330"
     CDS             complement(<1..312)
                     /locus_tag="VEL04_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004280929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYT47235.1"
                     /translation="MSAWRPDALLAGFEALELELPGDYDGAVVATLVRLPVPQAPRGA
                     VLYVHGFSDYFFQRHMAERFGLEGFAFHALDLRKHGRSLKPHQHPNFCKSVAEYYDDI
                     TQ"
     gene            complement(293..1339)
                     /gene="queG"
                     /locus_tag="VEL04_02335"
     CDS             complement(293..1339)
                     /gene="queG"
                     /locus_tag="VEL04_02335"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HYT47236.1"
                     /translation="MDYAALARRIKDWGAELGFQAVGIADADLSAAEPRLLEWLAQGW
                     HGEMEYMARHGALRSRPAELKPGTLRVIACRMDYLNDASREISGKGGKAQIARYARGR
                     DYHKVMRGRLQALCERIEGEVGAFGHRVFSDSAPVMEVELAARAGIGWRGKHTLLLSR
                     DAGSWFFLGEIYCDLPLPVDSPEKERCGTCQKCIDICPTQAIRGPYRLDARRCISYLT
                     IELKGAIPEELRPLIGNRVYGCDDCQLVCPWNRFAKLTPEKDFEVRNGLDNASLIELF
                     TWSETEFDERMRGSPIRRIGYARWLRNLAVGLGNAPTSPEVVKSLLSRKDHPSALVRE
                     HVAWALARHERLAA"
     gene            1338..1802
                     /gene="tsaE"
                     /locus_tag="VEL04_02340"
     CDS             1338..1802
                     /gene="tsaE"
                     /locus_tag="VEL04_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HYT47237.1"
                     /translation="MSLDLNLPDAAATHALGEALAAGVAPGRVLHLRGDLGAGKTTVV
                     RGLLRGLGYRGTVKSPTYTLVEPYSISRLNLYHFDLYRLKNRSEWSSSGFREYFGQDA
                     ACIVEWPERAGDALAPPDLEVRLEFAGEARRAALEARTPHGEAWLASLRLPA"
     gene            1835..2938
                     /locus_tag="VEL04_02345"
     CDS             1835..2938
                     /locus_tag="VEL04_02345"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HYT47238.1"
                     /translation="MWPAPEYTRLTLESTEELKFSLFSVKHPGRLVLDLETSELSVAL
                     VELNGKVAEEDPYIQGLRVGRNRPGVVRLVLDLKTEVKPQVFSLKPIGEYGHRLVLDI
                     YPVVPVDPLAALIEQAEKPKPGERPKVARLATIVIDAGHGGEDPGAIGRRGSREKHIT
                     LTIARRLKALVDAEPSMRAVLTREGDYFLPLDVRVEKARQVKADLFVSIHADAFVRPH
                     ARGSSVFALSDRRATSELARLLAKKENESDLIGGVNLAGKDDYLKRTIIDLSQTATID
                     YSLRLGNSVLQRLGSVNELHKPRVEQASFAVLKSPSVPSILVETAFISNPQEEKRLND
                     QAYQAKLARAILEGIRDYIARHPPRPASPLALN"
     gene            complement(2918..3553)
                     /locus_tag="VEL04_02350"
     CDS             complement(2918..3553)
                     /locus_tag="VEL04_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HYT47239.1"
                     /translation="MEFAASLWDLAVHLDVHLAAFVARHGAWVYGLLFAIVFCETGLV
                     VTPFLPGDSLLFVTGTLAAAGGMDIAVVMATLVGAALCGDNVNYWIGRWTGTRLFSKF
                     INPVYLQRTHDFYARHGGKTIVIARFVPIIRTYVPFVAGLGAMPYLRYLAFCVLGAAL
                     WVGSICLAGYFFGNIPAVKGNLTLVIIGIVALSISPGVIAWLRSRSVQRQG"
     gene            3515..5263
                     /gene="mutL"
                     /locus_tag="VEL04_02355"
     CDS             3515..5263
                     /gene="mutL"
                     /locus_tag="VEL04_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HYT47240.1"
                     /translation="MHREIPQRGGKFHGPILYTRRVRAIRVLPELLVSQIAAGEVVER
                     PASVLKELLENSLDAGARAIAVTLDAGGAKRVQVEDDGEGIGAAELRLALARHATSKI
                     ASLEDLEAVATMGFRGEALASIASVSRLSITSRSPQAQRASVVRAEGSRIAAVEPAAR
                     AQGTTVAVEDLYFNTPARRKFLRSEATEFGHCDEVFRRIALARPEIAFSLKHNARMSR
                     SLRAQPLAERMAALLGRELVEASVVVEVQAGALRLLGLAGTPQAAKARADSQYFFVNG
                     RFVRDRLLGHAAREAYSGLLHGERQPAYVLYLELDPRGVDVNVHPAKIEVRFRDARSV
                     HQFVRHALERALAPSAAEAPVAYASVSPGSPIQASFGLAQPAAAYESFMSVEKKAPPL
                     GYPLAQLHGVYILAQNDAGLVLVDMHAAHERIVMEKLKRDLDAGAVARQSLLVPAVLS
                     AEALDIATAEENREALERLGLEVSVSGPNELAVRAAPAPLAGGDVVELTRNVLREIRE
                     YGASQVLSARQNELLATMACHAAVRANRMLTTAEMNALLREMEETERSGSCNHGRPTW
                     YQLSLADLDRLFLRGR"
     gene            5265..6011
                     /locus_tag="VEL04_02360"
     CDS             5265..6011
                     /locus_tag="VEL04_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYT47241.1"
                     /translation="MPKVMLVTGAGRGIGAATARLAAERGYSVCVNYLRDEESARKVI
                     RELEKKGAKAIAVAGDVASEPDVIRLFDTVDRSLGRITALVNNAGIVDVRARVEEMTL
                     ERLSRMFAVNVIGSFLCAREAVKRMSLRRGGAGGAIVNLSSAAARLGSPGDYVDYAAS
                     KAAIDTFTLGLAKEVAGEGIRVNAVRPGVIRTEIHATSGDPGRLERIGATAPLARPGE
                     PEEVARAILWLLSDEASYTTGSVVDVTGGR"
BASE COUNT         1015 a         2091 c         2014 g          925 t
ORIGIN      
        1 ctgcgtgatg tcatcgtagt actcggcgac gctcttgcag aagttcggat gctggtgcgg
       61 cttgagcgag cggccgtgct tgcgcaggtc gagcgcgtgg aaggcgaagc cttccaggcc
      121 gaagcgctct gccatgtggc gctggaagaa ataatcgctg aagccgtgca catacagcac
      181 ggcgccgcgc ggcgcctgcg gcaccggcag gcgtaccagc gtcgccacca ccgcgccgtc
      241 gtaatcgccg gggagctcga gctcgagcgc ctcgaagccg gcgagcagcg cgtcaggccg
      301 ccaggcgctc atgcctggcc agggcccatg cgacgtgctc tcgtaccagg gcggacggat
      361 gatccttcct tgaaagaagc gacttcacca cttcaggcga cgtcggggcg ttgccgaggc
      421 caacggcgag gtttctcagc cagcgcgcgt agccgatgcg ccggatcggc gagccgcgca
      481 tgcgctcgtc gaactcggtt tcgctccacg tgaacaattc gatcagcgaa gcgttatcca
      541 atccattcct tacttcgaag tccttctcag gggtgagttt cgcaaaacgg ttccatgggc
      601 ataccagctg gcagtcatcg cagccgtaaa cgcggttgcc gatgagcggc cgcagctcct
      661 cggggatcgc gcctttcagc tcgatcgtaa ggtacgagat gcagcgccgc gcgtcgagcc
      721 ggtaaggccc gcggatcgcc tgcgtcgggc agatgtcgat gcacttctgg caggtgccgc
      781 agcgctcctt ctcgggggaa tccaccggca gcggcaggtc gcagtaaatc tccccgagga
      841 aaaaccacga gcccgcgtcc cgcgacagca gcagcgtgtg cttgcctcgc cagccgatcc
      901 cggctcttgc cgcgagctcg acttccatca ccggcgccga gtccgagaac acgcggtggc
      961 cgaaggcgcc gacctcgcct tcgatgcgct cgcacagcgc ctgcagccgg ccacgcatca
     1021 ccttgtggta gtcgcggccg cgagcatagc gggcgatctg cgctttgccg cctttcccgg
     1081 agatctccct cgaagcatcg ttcaggtaat ccatgcggca agcgatcacg cgcaaggtgc
     1141 cgggcttgag ctcggccggc ctcgagcgta gcgcgccatg gcgcgccata tactccatct
     1201 ccccatgcca gccctgcgcc agccattcca gcaaacgcgg ctcggccgcc gacagatcgg
     1261 catcggcgat gcccaccgcc tggaagccga gctcggctcc ccagtccttg atgcgtcgcg
     1321 ccagcgcggc gtagtccatg tcgctcgatc ttaatttgcc ggatgcggcc gcgacgcatg
     1381 cgctgggcga agcgcttgcc gcaggagtcg cgccaggccg ggtgctgcac ctgcgcggcg
     1441 atctgggcgc cgggaaaacc accgtggtgc ggggcttgtt gcgcggcctc ggctaccgcg
     1501 gcacggtcaa aagtcctacc tacaccttgg ttgaacctta tagcatttcg agattaaact
     1561 tgtatcactt tgatttatat cggctgaaga atcgatcaga atggtccagc tcaggcttca
     1621 gggaatactt cgggcaagac gcggcctgca tcgtcgaatg gccggagcgt gccggggacg
     1681 cgctcgcccc gccggatctc gaagtgcgcc tcgagtttgc cggcgaagcg cggcgcgcgg
     1741 cgctcgaggc gcgcacgccg catggcgaag cctggcttgc gtccttgcgg ttgcctgcgt
     1801 aagcgcggca caggcgcagg tcgtcgcgac acgcatctgg cccgcgcccg aatacacgcg
     1861 cctcacgctc gagtcgaccg aggagctgaa attcagcctc ttcagcgtca agcaccccgg
     1921 gcggctggtg ctcgatctcg aaacctccga gctgtcggtg gcgctcgtcg agctcaacgg
     1981 caaggtcgcc gaggaagacc cctacatcca gggcctgcgc gtcggccgca accggcccgg
     2041 cgtggttcgc ctggtgctcg acctcaagac cgaggtgaag ccgcaggtgt tcagcctgaa
     2101 gccgatcggc gagtacggtc atcgcctggt gctcgacatc taccccgtgg tgccggtcga
     2161 tccgctcgcc gcgctgatcg agcaggctga aaagccgaag cctggcgagc ggcccaaggt
     2221 cgcgcgcctc gcgaccatcg tgatcgacgc cggccacggc ggcgaggacc ccggggccat
     2281 cggccgccgc ggctcgcgcg agaagcacat cacgctcacc atcgctcgcc gcctgaaggc
     2341 gctggtcgac gccgagccca gcatgcgggc cgtgctcacc cgcgagggcg attacttcct
     2401 gccgctggac gtgcgcgtcg agaaagcgcg gcaggtcaag gccgatctct tcgtttcgat
     2461 ccacgccgac gccttcgtgc ggccgcatgc gcgcggctcc tccgtgttcg cgctctcgga
     2521 ccggcgcgcg acctccgagc tggcgcgcct cctggcgaag aaggaaaacg agtccgacct
     2581 gatcggcggc gtcaacctgg cgggcaagga cgactacctg aagagaacca tcatcgatct
     2641 ctcccagacc gcgaccatcg actacagcct gcggctcggc aactccgtgc tgcagcggct
     2701 cggctcggtc aacgagctgc acaagccccg cgtcgagcag gcgagcttcg ccgtcctcaa
     2761 atcgcccagc gtgccgtcga tcctggtcga gaccgccttc atttccaatc cgcaggaaga
     2821 aaagcggctc aacgaccagg cttaccaggc caagctcgcg cgtgcgatcc tcgaaggcat
     2881 ccgcgactac atcgcacgcc acccgccgcg gccggcctca cccctggcgc tgaactgagc
     2941 ggctgcgcag ccaggcgatc acgcccggcg atatcgagag cgcgacgatg ccgatgatca
     3001 ccagggtgag gttgcccttc accgccggaa tgttgccgaa gaaatagccg gcgaggcaga
     3061 tcgaccccac ccacagcgcc gcgccgagca cgcagaacgc gaggtagcgc aggtacggca
     3121 tcgctcccag gccggcgacg aacggcacat aggtgcgaat gatcggcacg aacctcgcga
     3181 tgacgatggt cttgccgccg tggcgcgcgt aaaagtcatg cgtgcgctgc aggtagacgg
     3241 ggttgatgaa ttttgaaaac aacctggttc ccgtccagcg cccgatccag tagttgacgt
     3301 tgtcgccgca cagcgccgcg ccaaccagcg tcgccatgac gaccgcgatg tccatgccgc
     3361 ccgcggcggc cagggtgccg gtgacgaaca gcagcgaatc ccccggcagg aacggcgtca
     3421 ccaccagccc ggtttcgcag aacacgatcg cgaacagcag cccgtaaacc catgcgccgt
     3481 ggcgcgcgac gaacgccgcg agatgcacgt cgagatgcac cgcgagatcc cacagcgagg
     3541 cggcaaattc catgggccga ttctatatac tcggcgcgtg cgcgccattc gcgtcctgcc
     3601 cgagctgctg gtcagccaga tcgccgccgg cgaggtggtc gagcggccgg catcggtgct
     3661 gaaggagctg ctcgagaaca gcctcgatgc cggcgcgcgc gcgatcgccg tcacgctcga
     3721 cgcaggcggc gcgaagcgcg tgcaggtcga ggacgacggc gagggcatcg gcgccgcaga
     3781 gctgcgcctc gcgctcgcgc gccacgccac cagcaagatc gcgagcctcg aggatctcga
     3841 ggcggtggcg accatggggt ttcgcggcga ggcgctggcc tcgatcgcct cggtgtcgcg
     3901 cctttcgatt acctcgcgca gcccgcaggc gcagcgtgcg agcgtggtcc gcgcggaagg
     3961 ctcgcgcatc gccgcggtcg agcccgcggc ccgcgcccag ggcaccacgg tcgcggtcga
     4021 ggacctctat ttcaataccc cggcgcggcg caagttcctg cgcagcgagg cgaccgagtt
     4081 cggccactgc gacgaagtgt tccgccgcat cgctctggcg cgtcccgaga tcgcgttcag
     4141 cctgaagcac aatgcgcgca tgagccgcag cctgcgcgcc cagccgctcg ccgagcgcat
     4201 ggccgctttg ctcgggcggg agctggtcga ggcgagcgtc gtggtcgagg tccaggccgg
     4261 tgcgctgcgc ctgctcggtc tggccggcac gccgcaagcg gcaaaagcgc gcgccgactc
     4321 gcagtatttc ttcgtcaacg gccgcttcgt gcgcgaccgc ctgctcgggc acgccgcgcg
     4381 cgaggcctac tcgggcctgc tgcacggcga gcgccagccg gcgtacgtgc tctacctcga
     4441 gctcgatccg cgcggggtcg acgtcaacgt gcacccggcc aagatcgagg tgcgctttcg
     4501 cgacgcgagg agcgtgcacc agttcgtgcg ccacgccctc gagcgcgcgc tcgcgccgag
     4561 cgccgccgag gcgcccgtcg cctacgccag cgtttccccg ggctctccca tccaggcctc
     4621 gttcggcctt gcacagcccg ctgccgctta tgagtcattc atgtctgttg aaaaaaaagc
     4681 cccgcccctt ggctatccgc tcgcccagct gcacggcgtc tacatcctgg cccagaacga
     4741 cgccgggctg gtgctggtcg acatgcacgc cgcgcacgag cgcatcgtga tggaaaagct
     4801 gaagcgcgat ctcgacgccg gtgcagtggc gcgccagagc ctgctggtcc ccgcggtgct
     4861 gagcgccgag gcgctcgaca tcgccaccgc cgaggagaac cgcgaggccc tcgagcgcct
     4921 cgggctggaa gtcagcgtca gcgggccgaa cgagctcgcg gtgcgcgccg cgcctgcgcc
     4981 ccttgccggc ggcgatgtgg tcgaattgac gcgcaacgtg ctcagggaga tcagggagta
     5041 cggcgcgagc caggtgttgt cggcgcggca aaacgagctg ctcgcgacca tggcctgcca
     5101 tgccgcggtg cgcgccaacc gcatgctgac cacggcggag atgaacgccc tcctcaggga
     5161 aatggaggag accgagcgct caggaagctg caaccacggc cggccgacgt ggtaccagct
     5221 gtcgctcgcc gacctcgacc ggctgttcct gcgcgggcgc tgagatgccc aaggtgatgc
     5281 tcgtgaccgg cgcaggacgc ggcatcggcg cggcgaccgc gcgccttgcg gctgaacgcg
     5341 gttattcggt ctgcgtcaac tatttgcgcg atgaagagtc ggcccgcaag gtcatcagag
     5401 agctcgaaaa gaaaggagca aaggcaattg cggtagcggg cgacgtcgct tcggagcccg
     5461 atgtcatacg cctgttcgat accgtcgacc gctcattggg acgcattacg gcgctggtca
     5521 ataacgcggg gatcgtcgat gtgcgcgcac gcgtcgagga gatgacgctc gagcggctga
     5581 gccgcatgtt cgcggtgaac gtgatcggca gcttcctctg cgcgcgcgag gccgtgaagc
     5641 gcatgtcgct gcgccgcggt ggcgccggcg gcgcgatcgt caacctttcg tcggccgccg
     5701 cgcggctcgg cagcccaggg gactacgtgg actacgccgc gagcaaggcc gcgatcgaca
     5761 ccttcacgct cggcctggcg aaggaagtcg ccggcgaagg catccgggtg aatgccgtgc
     5821 ggccaggggt gatccgcacc gagatccatg cgacgagcgg cgatccgggc cggctcgagc
     5881 gcatcggcgc gaccgccccg ctcgcccggc ccggggaacc cgaggaagtc gcgcgcgcca
     5941 ttctctggct gctgtccgac gaggcgtcct acacgaccgg ctcggtggtg gacgtcaccg
     6001 gcggccgcta gccttgccgc gcaccgcctt cgccctgctc ggccc
//
LOCUS       DASPXW010000101         5288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_282846, whole genome shotgun sequence.
ACCESSION   DASPXW010000101 DASPXW010000000
VERSION     DASPXW010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5288
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282846"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VEL04_02365"
     CDS             <1..237
                     /locus_tag="VEL04_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYT47242.1"
                     /translation="YDWQAGDVAIVHNNCVHQHFNASPDKPARALVIKTKPMYMFMNM
                     LFQQSVIPRPREPAGPAGAGFEPRHGETSFNHDQ"
     gene            234..1349
                     /locus_tag="VEL04_02370"
     CDS             234..1349
                     /locus_tag="VEL04_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045735 - nutrient reservoir activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYT47243.1"
                     /translation="MKTAAVSEDFARKFASEKETPYTRWVRDQGLEIISSFYVPDLHA
                     VELKPWPRRGGRGVFLNHDASRTSNDCYVCEIPRGKALAPQRQLYEEMIYVLDGRGST
                     AVWNNAGKKISFEWKAGAIFAIPLNAWHQHFNGSGENAARYVAVTNAPVIINAFGDLD
                     FVFNTDYDFKDRFDGEPEYFANKGEQKGLLLDTNFVADAVNLPLFAAKERGAGGGHTR
                     FSMAKGSMNSHISQFPVGTYKKAHAHGPGAHVIVLSGEGFSLMWPEGEEPRRYDWREG
                     AMIVPPNLWFHQHFNTGTTPARYLAFKAEGVAIRNAQGVPKAWISKRLGGDQIDYADE
                     RHEIRQWFADALAKHGLKSNMDKAYTAERGELPEKAA"
     gene            1438..2091
                     /locus_tag="VEL04_02375"
     CDS             1438..2091
                     /locus_tag="VEL04_02375"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HYT47244.1"
                     /translation="MISDLSVHVVQRGHDRRACFFDVGDYLAYLDYLQFFAARFGCSV
                     HAYCLMTNHVHLLMTPGTSDGCALLMKHLGQRYVQRINRKLNRTGTLWEGRFHSCLVG
                     SEHYALACYRYIELNPVRAGMAGHPREYRWSSYGVNSEGQGDGFLRPHPAYVALADDY
                     RRRGYAYSTLCETSLEDSVIEELRKATRGGYLVGAVRRRRGRPAVMRKIGSVPIFKP"
     gene            complement(2081..3361)
                     /locus_tag="VEL04_02380"
     CDS             complement(2081..3361)
                     /locus_tag="VEL04_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HYT47245.1"
                     /translation="MRAEQNEFLTRTGPGTPMGELFRRYWLPALLAEELPAPDCPPLR
                     VKLLSERLIAFRDTQNRLGLVDEFCAHRGVSLWFGRNEENGLRCSYHGWKYDVTGQCV
                     EIPSEPDNPKLCRRMKLKSYPLIERGGVLWTCMGPADQRPPLPEHEWAMVPAPNRFVS
                     KRWQESNWLQAMEGGIDSSHVSFLHRHTMTVDPLFKGAKGNEYNLRDLKPHFEVVESP
                     GGLYIGARRDTGDGKYYWRITQWVMPSFTLIPPRGDHPIGGHSWVPMDDENCWAWSTN
                     HHAMRPLTHAEREALEAGKGIHVPLIPGTFRPLANKDNDYLMDRSAQKDGGSFSGVEG
                     FAMQDASVQESMGPIQDRTRENLVPTDQGIIMARRRLVKAAEQLGKGVTPPGTDPSQQ
                     RVRSVSIILPQGVAFSEAAEAALVPGPGSAHATV"
     gene            complement(3358..4512)
                     /locus_tag="VEL04_02385"
     CDS             complement(3358..4512)
                     /locus_tag="VEL04_02385"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYT47246.1"
                     /translation="MAVYLVVLLSVLSSISQRGAKVAVSLYALELGAGSLAVGVLAAM
                     FAAFPLLLAVHAGRASDRFGVRYPIVLGAATMALGLAAPSVAGGFAALLLCAALVGLG
                     HIFFHVSIHNLVGSLGTASERTRNFSTFALGSSIAAFIGPSAAGFGIELGGYPATFLL
                     LAVVAAFPALLVIAKRNLVPAHVGHEEAERARSAWDLLANRGLRHTLIMSGVTLTGIE
                     LFSFYLPIYGRAIGLAPSSIGMILSTYAVAGFIVRAFMHRLAERFTEVGVLTGSLFVA
                     AVAYAAVPGLTDGMFLAAAAFALGLALGCAQPLTIILTYNHAPRGRSGEALGMRIMAN
                     KVTQISMPLLFGGLGAALGSVPVFLATGAFLLTGGVLSLVNFLRMREPRS"
     gene            4647..5111
                     /locus_tag="VEL04_02390"
     CDS             4647..5111
                     /locus_tag="VEL04_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47247.1"
                     /translation="MIALQPAAQRACGSCTACCDGWIAGTIYGHEMKPGKACHFRGEG
                     CCTIYDKRPADPCRNFVCGWLAADSPFPESFRPDRLGVMIVGTRWRRQPAFILCSAGR
                     DPDERLLAWMREFADRSGAPFFYEQSGERFGFGPPEFQQEMLGKLERGERLW"
BASE COUNT          951 a         1711 c         1788 g          838 t
ORIGIN      
        1 tacgactggc aggcgggcga cgtcgcgatc gtgcacaaca actgcgtgca ccagcacttc
       61 aacgccagcc ccgacaagcc ggcgcgcgcg ctggtgatca agaccaagcc gatgtacatg
      121 ttcatgaaca tgctgttcca gcaatccgtg atcccgcggc cgagagagcc agccggtcct
      181 gccggcgccg gcttcgagcc gcggcacggc gagacgagct tcaaccacga ccaatgaaga
      241 ccgctgcagt ctcggaagac ttcgcccgca agttcgcctc cgagaaagag acgccttata
      301 cgcgctgggt gcgcgaccag ggtctggaga tcatcagctc gttctacgtg ccggatctgc
      361 acgccgtgga gctcaagccc tggcccaggc gcggcgggcg cggcgtgttc ctcaaccacg
      421 acgcctctcg tacctcgaac gactgctacg tctgcgagat ccctcggggg aaagcgcttg
      481 cgccgcagcg gcagctctac gaggagatga tctacgtgct ggatgggcgc ggctcgaccg
      541 cggtctggaa caatgcgggg aagaaaatca gcttcgagtg gaaggcggga gcgatcttcg
      601 ccatcccgct caatgcctgg caccagcact tcaacggctc gggcgagaac gcggcgcgct
      661 acgtcgcggt gaccaacgcg ccggtgatca tcaatgcctt cggcgatctc gatttcgtct
      721 ttaacaccga ttacgacttc aaggaccgct tcgacggcga gcccgagtac ttcgccaaca
      781 agggcgagca gaagggcctg ctgctcgaca ccaatttcgt cgccgacgcg gtgaacctgc
      841 cgctgttcgc cgcgaaggag cgcggcgcgg gcggcgggca cacgcgcttc agcatggcga
      901 agggctcgat gaacagccac atctcgcagt tcccggtcgg cacctacaag aaggcgcatg
      961 cccatggccc cggcgcgcac gtcatcgtgc tgagcggcga gggcttcagc ctgatgtggc
     1021 cggaaggcga ggagcccagg cgctacgact ggcgcgaggg cgcgatgatc gtgccgccga
     1081 acctctggtt ccaccagcac ttcaacaccg gcaccacgcc ggcgcgctat ctcgccttca
     1141 aggccgaagg cgtcgcgatc cgcaatgcgc agggcgtacc gaaagcatgg atcagcaagc
     1201 gccttggcgg cgaccagatc gactacgccg acgagcgcca tgaaatcagg cagtggttcg
     1261 ccgatgcgct ggcgaagcac ggcctgaagt cgaacatgga caaggcctac acggccgaac
     1321 gcggcgagct gccggaaaaa gccgcttaaa tggggacaga ccccattttt ctcatttagc
     1381 cgtcaacctc cagcgctgtt gtgccgtttc ggcgttatgc cgcgtacggc gcgcgtagtg
     1441 atctccgacc tcagcgtgca cgtcgttcag cgcgggcacg atcgccgcgc gtgcttcttc
     1501 gacgtgggcg attaccttgc ctacctggac taccttcagt ttttcgcagc acgctttggg
     1561 tgctcggtgc acgcgtactg cttgatgacc aaccacgtgc atctgctgat gacgcccggc
     1621 acgtctgatg gctgcgctct tctcatgaag catcttggcc agcgctatgt gcagcggatc
     1681 aacagaaaac tcaaccggac aggcactctc tgggaaggac gtttccattc ttgcctcgtg
     1741 ggctcggagc actatgcgct cgcctgctac cgctatatcg aactcaatcc ggtgcgcgcc
     1801 ggcatggcag gacacccgcg cgaatatcgg tggtcaagct acggcgttaa ctccgaaggg
     1861 cagggcgatg ggttcctaag gccgcatccg gcgtatgtcg cgcttgcgga tgattaccga
     1921 cgccgcgggt acgcttacag cacactctgc gagacgtcgc tcgaagatag cgtcatcgag
     1981 gaacttcgca aagccactcg cggcggctat ctcgtcggtg cggtgcgcag gcgccggggt
     2041 cggccagcgg taatgagaaa aatagggtct gtccccattt tcaaaccgta gcgtgggcgg
     2101 agccgggccc gggaacgagc gcggcttcgg cggcttcgct gaacgccacg ccttgcggca
     2161 ggatgatcga gaccgagcgc acgcgctgct ggctcggatc ggtacccggc ggcgtgacgc
     2221 ccttgccgag ttgctcggcg gccttcacca ggcgccggcg cgccatgatg atgccctggt
     2281 cggtcggcac caggttctcg cgcgtgcggt cctggatcgg ccccatgctc tcctggactg
     2341 aagcgtcctg catcgcgaag ccctcgacac ctgagaagct gccgccgtcc ttctgggcgc
     2401 tgcggtccat cagatagtcg ttgtccttgt tggcgagcgg gcggaaggtg ccggggatga
     2461 gcggcacgtg gatgcctttt ccggcctcga gcgcctcgcg ctccgcatgc gtgagcgggc
     2521 gcatggcgtg atggttggtg ctccatgccc agcagttctc gtcgtccatc ggcacccagg
     2581 aatggccgcc gatcggatgg tcgccgcgcg gcgggatcaa tgtgaagctc ggcatcaccc
     2641 actgcgtgat gcgccagtag tacttgccgt cgccggtgtc gcgccgcgca ccgatgtaca
     2701 gcccgcccgg ggactcgacc acctcgaagt gcggcttcag gtccctgagg ttgtactcgt
     2761 tgcccttggc gcccttgaac agcggatcga ccgtcatggt gtggcggtgc aggaacgaga
     2821 cgtggctcga gtcgatgccg ccttccatcg cctgcagcca gttggactcc tgccagcgct
     2881 tggacacgaa gcggttcggc gccggcacca tcgcccactc gtgctcgggc aggggcgggc
     2941 gctggtcggc cgggcccatg caggtccaca gcacgccgcc gcgctcgatc agcggatagg
     3001 acttgagctt catccgccgg cacagcttcg gattgtcggg ctccgagggg atctcgacgc
     3061 actggccggt gacgtcgtac ttccagccgt gataggagca gcgcaggccg ttttcctcgt
     3121 tgcggccgaa ccatagcgag acgccgcggt gcgcgcagaa ctcgtcgacc aggccgaggc
     3181 ggttctgcgt gtcgcgaaac gcgatcaggc gctcggacag cagcttcacg cgcagcggcg
     3241 ggcagtcggg cgccggcagc tcctcggcga gcagcgccgg cagccagtag cgcctgaaaa
     3301 gctcacccat cggcgtgccg gggccggtgc gcgtaaggaa ttcgttctgt tccgctctca
     3361 tgaacgcggt tctcgcatac gcaggaagtt taccagcgac agcacgccgc ccgtgagcag
     3421 gaaggcgccg gtggcgagga acaccggcac cgagccgagc gcggcgccga ggccgccgaa
     3481 cagcagcggc atgctgatct gcgtcacctt gttcgccatg atgcgcatgc cgagcgcctc
     3541 gcccgagcgt ccccgcggcg cgtggttgta ggtgaggatg atggtgagcg gctgcgcgca
     3601 gccgagcgcg aggccgagcg cgaacgcggc tgcggcgagg aacatgccgt cggtcaggcc
     3661 gggcaccgcg gcataggcga ccgcggcgac gaacaacgac ccggtgagca cgccgacctc
     3721 ggtgaagcgc tcggcgaggc gatgcatgaa ggcccgcacg atgaagccgg ccacggcgta
     3781 ggtcgacagg atcatgccga tcgaggaggg cgcgagcccg atggcgcggc cgtagatcgg
     3841 caggtagaac gagaacagct cgatcccggt gagcgttacc ccgctcatga tcagcgtatg
     3901 ccgcaggccg cggttcgcga gcaggtccca cgcgcttcgc gcgcgctccg cttcttcgtg
     3961 ccccacgtgc gcgggaacca gatttctttt ggcgattaca agcaaggcgg gaaacgcggc
     4021 cacgaccgcg agcagcagga aggtcgccgg gtagccgccg agctcgatgc cgaatccagc
     4081 ggccgaaggt ccgatgaacg ccgcgatcga gctgccgagc gcgaacgtcg agaagtttct
     4141 ggtgcgctcg gatgccgtgc ctagcgagcc gaccaggtta tggatcgaga cgtggaagaa
     4201 gatgtggccg agcccgacca gcgcggcgca cagcaggagg gcggcgaagc cgccggcgac
     4261 actcggtgca gcgaggccga gcgccatggt ggctgcaccg agcacgatcg gatagcgtac
     4321 gccgaaccgg tcggaggccc tgccggcgtg caccgcgagc agcagcggaa acgcagcgaa
     4381 catcgccgcg agcacgccca ccgcgagcga gcccgcgccg agctcgagcg catagagcga
     4441 caccgcgacc ttggctccgc gctggctgat cgagctcagc accgacagca ggacgacgag
     4501 gtagacagcc atcggtgcgg cgttccccta tgcgggacgt gattctgagg gacggcacga
     4561 gtatactgaa cgccgccatg caggtggcgc gaaacgatcc atgtccttgc ggcagcggga
     4621 aaaaatacaa gaagtgttgc ggcgcggtga tcgcgctgca gccggccgcg caacgcgcct
     4681 gcggcagctg caccgcgtgc tgcgacggct ggatcgcggg cacgatttac ggtcacgaga
     4741 tgaagcccgg caaggcctgc cacttccgtg gcgagggctg ctgcacgatc tacgacaagc
     4801 gcccggccga tccctgccgc aatttcgtgt gcggctggct cgcagccgac agcccgttcc
     4861 cggagagctt ccggccggac cggctcggcg tgatgatcgt aggcacgcgc tggcgcaggc
     4921 agccggcgtt catcctgtgc tcggcgggcc gcgatccgga cgagaggctg ctcgcctgga
     4981 tgcgcgagtt cgcggaccgc agcggcgcgc cgttcttcta cgagcagagc ggcgagcgct
     5041 tcggcttcgg tccgcccgag ttccagcagg agatgctcgg caagctggag cgcggcgaga
     5101 ggctgtggtg acgcaagatg gcaactgaat cgcactatca cagcaagaag gaccgcatct
     5161 tcgtgcgcgc catccagggc cagtacagcc tgcgcgagga gctggcgcgg ctgcgcgcca
     5221 tgccgcgcgt gcgcaagggc aggcagatca agttcgtcga cggcccgcag acctacagcc
     5281 ggcattac
//
LOCUS       DASPXW010000102         4377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1420064, whole genome shotgun sequence.
ACCESSION   DASPXW010000102 DASPXW010000000
VERSION     DASPXW010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1420064"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1105
                     /locus_tag="VEL04_02395"
     CDS             <1..1105
                     /locus_tag="VEL04_02395"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYT47248.1"
                     /translation="GFQSFIGQSCQKGAELAPGVIRDMLGVDIELMNADTETNVDTAR
                     TRAERLIQEGAHVLVGPFDSGAAAAIAQVAEQRGIPFVINIAAAPQITEQGYKFVFRN
                     FQTAPDIVRNGLALIGDLFQATNTVPRSAVFMHVNDTFGQAMAKGIGAFMPRLTQLPF
                     KIVDTISYDPAAKDLTVEVSKAKAANADFLLLVCRLNDAILLRREMVKQRWNPMGIVS
                     PGSPGMYEEQYFKTLGKLSEHSISNVPWYNPRAELTKVVEKAFKKLNPKDRLEFHGLN
                     AGFTFEAILIAADAFKRARSTDPKTLADAIRGTHITNKMSLGGPIRFNDKGQVEGIGS
                     ACVQNLNLTPTVVLPAASATAKPVFPVPGYKKA"
     gene            1106..1975
                     /locus_tag="VEL04_02400"
     CDS             1106..1975
                     /locus_tag="VEL04_02400"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYT47249.1"
                     /translation="MSFDLVANVVIAGVLTGLVYGLMALGLSVIFGVVRVVNFAHGEM
                     MTIAMYAATVLFAALKLDPFLAVLPVAAAFFVFGCALQASLINPFITRPEHSQFMLLV
                     AVAIILVNAMLMVFGPDARNVQVDYQLESFELGRILVDEARFYAAAAAIVAAAALFAF
                     FKFSLTGKAIRACADNYLGARIVGLNVKRLYSLTFGIGASCVAVAGCAMVLLVEVTPA
                     LGPAYTLLAFVIVIVGGLGSMAGALLGGVLIGVSEALAGLLIAPSAKSMFSFGLLILV
                     LLFRPQGLLGRKS"
     gene            1972..2931
                     /locus_tag="VEL04_02405"
     CDS             1972..2931
                     /locus_tag="VEL04_02405"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYT47250.1"
                     /translation="MKRHLALGILLAVLVALPAFCGNYALSVATLILYFAYTGQAWNV
                     MMGFAGQLSLGHALYVGVGGYAAGALFFHWGIGPWAGLWVAMLLCAALGVVIGFLAFR
                     FGISGVYFALLTIAFAEFTRIGFDHFDWLGGPGGMFLKVAQRDSWDLANFRGPPLMYY
                     YAMLALAAGAFVLCFFLLKSRAGYYWQAIRENEEAAQALGINTFRWKMLAVALSAAMT
                     SIAGVFSAFYYNNLFPEQVFSIGRSIEIILGPVIGGVGTLFGPILGAVALTVLSDGIT
                     EALAALGWEIPGIKQVFYGVVLLAVIMFLPHGIWPALARKLRL"
     gene            2928..3650
                     /locus_tag="VEL04_02410"
     CDS             2928..3650
                     /locus_tag="VEL04_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT47251.1"
                     /translation="MSLEIRGLDKSFRGLKAVSQASFSVAHGDIVGLIGPNGAGKTTI
                     FNMVAGVYAPDSGEIIFNRRKIQGLRADQVCAAGIGRTFQIVKPFAGLSVLDNVMVGG
                     FLREKNTSGARALSISILDQLGLGPKRDLPASSLTLPDRKRLEVARALATRPRLLLLD
                     EVMAGLRPTECDQMVEVFRELNRADGLTIVLIEHVMRAVMALARDIVVLHHGEVIARG
                     TPAEVVRDRAVLECYLGEETEI"
     gene            3647..4360
                     /locus_tag="VEL04_02415"
     CDS             3647..4360
                     /locus_tag="VEL04_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT47252.1"
                     /translation="MTAFLQIENLNLYYGDAQALSDVSLEVPRGEIVAIVGANGAGKS
                     SLIRTIAGIEKRRSGKIVFNEADISVLESHAICNLGIGQVAEGRQVFPSLTVFENLEV
                     GAMLPRARRSARRLLDQVFALFPRLRERTAQVAGTLSGGEQQMLAIGRCLMGAPELVM
                     FDEPSLGLAPTVVQAVLATIRDLNRDGLTCVLVEQNVAVSLRLANRAYVLENGRITLA
                     GSGAELLADDRVRQAYLGL"
BASE COUNT          673 a         1475 c         1398 g          831 t
ORIGIN      
        1 cggattccag agcttcatcg gccagtcgtg ccagaaaggc gccgagctcg ccccgggcgt
       61 gatcagggac atgctcgggg tcgacatcga gctgatgaac gccgacaccg agactaacgt
      121 cgataccgcg cgcacgcgcg ccgagcggct gatccaggaa ggcgcccacg tgctggtcgg
      181 gccgttcgac tcgggcgccg ccgcggcgat cgcgcaggtc gccgagcagc gcggcatccc
      241 gttcgtcatc aacatcgccg cagcgccgca gatcaccgag cagggctaca agttcgtgtt
      301 tcgcaacttc cagaccgcgc ccgacatcgt gaggaacggg ctggcgctga tcggcgacct
      361 gttccaggcg accaacaccg tgccgcgcag cgcggttttc atgcacgtca acgatacttt
      421 cggccaggcg atggcgaagg gcatcggcgc cttcatgccg cgcctcacgc agctgccgtt
      481 caagatcgtc gataccattt cctacgaccc cgcggcgaag gacctgaccg tggaagtctc
      541 caaggcgaaa gccgccaacg ccgactttct ccttttggtg tgccggttga acgacgccat
      601 cctcctgcgg cgcgagatgg tgaagcagcg ctggaacccg atgggcatcg tcagcccggg
      661 ctcgccggga atgtacgagg agcagtactt caagaccctc ggcaagctct ccgagcattc
      721 gatctccaac gtgccctggt acaacccgcg cgccgagctc accaaggtgg tcgaaaaggc
      781 gttcaagaag ctgaacccga aggaccgcct tgaattccac ggcttgaacg ccggcttcac
      841 cttcgaggcg atcctgatcg ccgccgacgc cttcaagcgc gcgagaagca ccgacccgaa
      901 gacgctggcc gacgcgattc gagggaccca catcaccaac aagatgtcgc tcggcgggcc
      961 gatcaggttc aacgacaaag gccaggtcga gggcatcggc tcggcctgcg tgcagaacct
     1021 gaatctgacg cccaccgtgg tgctgccggc cgcgtcggcg acggcgaagc cggtgttccc
     1081 ggttccgggg tacaagaagg cgtagttgtc ttttgatctc gtagcaaacg tcgtcatcgc
     1141 cggcgtcctg acggggctgg tgtacggcct gatggcgctc ggcctgtcgg tgatcttcgg
     1201 cgtggtgcgc gtggtcaatt tcgcccacgg cgagatgatg acgatcgcga tgtacgcggc
     1261 gacggtgctg ttcgcggcgc tgaagctcga tcccttcctc gcggtgctgc cggtggcggc
     1321 ggcgttcttc gtcttcggct gcgcgctgca ggcgagcctc atcaacccgt tcatcacgcg
     1381 tcccgagcac tcgcagttca tgctgctcgt cgcggtggcg atcatcctgg tgaacgccat
     1441 gctgatggtg ttcggccccg acgcgaggaa cgtgcaggtc gactaccagc tcgaatcgtt
     1501 cgagctcggc aggatcctcg tcgacgaggc gcgcttctat gccgccgccg ccgcaatcgt
     1561 cgctgctgcg gcgcttttcg cctttttcaa attcagcttg accgggaaag ccatccgcgc
     1621 gtgcgccgac aactatcttg gcgcccgaat agtgggtctg aatgtaaaga gactttattc
     1681 attgaccttc ggcatcggcg cgagctgcgt cgcagtcgca ggctgcgcca tggtgctgct
     1741 cgtcgaggtg acgccggcgc tcggcccggc ctatacgctg ctcgccttcg tcatcgtgat
     1801 cgtcggcggc ctcggctcca tggctggagc gctgctcggc ggcgtgctga tcggcgtttc
     1861 cgaggcgctc gccgggctgc tgatcgcgcc ttccgcgaag agcatgttca gcttcggcct
     1921 gctgatcctg gtgctgctct tccgcccgca gggattgctc ggcaggaagt catgaaaagg
     1981 catctggcgc tggggattct tctcgccgtt ctcgttgccc tgccggcctt ttgcggcaat
     2041 tacgccctgt cggtcgccac gctgatcctg tacttcgcct acaccggcca ggcgtggaac
     2101 gtgatgatgg gcttcgccgg ccagctctcg ctcgggcatg cgctctatgt cggcgtcggc
     2161 ggctatgccg cgggggcgct gttcttccac tggggcatcg ggccctgggc cgggctctgg
     2221 gtggcgatgc tgctgtgcgc ggcgctcggc gtggtgatcg gctttctcgc cttccgcttc
     2281 ggcatctccg gcgtgtactt cgcgctcctc accatcgcat tcgccgagtt cacgcgcatc
     2341 ggcttcgacc acttcgactg gctcggcggc cccggcggca tgttcctcaa ggtcgcgcag
     2401 cgcgacagct gggacctcgc caatttccgc ggcccgccgc tgatgtatta ctacgcgatg
     2461 ctcgcgcttg ctgccggcgc tttcgtcctg tgtttttttc tccttaaatc aagggcgggt
     2521 tattactggc aggccatccg cgaaaacgaa gaggccgcgc aggcgctcgg catcaacacc
     2581 ttccgctgga agatgctcgc ggtcgcgctg agcgccgcga tgacctcgat cgcgggcgtg
     2641 ttctccgcct tctactacaa caaccttttc cccgagcagg tattcagcat cggccgctcg
     2701 atcgagatca tcctcgggcc ggtgatcggc ggcgtgggaa cgctgttcgg cccgatcctc
     2761 ggcgccgtcg cgctcaccgt cctgtccgac ggcatcaccg aggcgctcgc cgcgctcggc
     2821 tgggagatcc cggggatcaa gcaggtgttc tacggcgtgg tgctgctcgc ggtcatcatg
     2881 ttcctgccgc acggtatctg gcctgccctc gcccggaagc ttcgcctgtg agcctggaaa
     2941 tcagagggct cgacaagtcc tttcgcgggc tgaaggcggt ctcgcaggcg agcttcagcg
     3001 tggcgcacgg cgacatcgtc ggcctgatcg ggccgaacgg cgccgggaaa accaccattt
     3061 tcaacatggt cgcgggcgtg tacgcgcccg actcgggcga gatcattttc aaccggagga
     3121 aaattcaggg cctgcgtgcc gaccaggtgt gcgccgcggg catcggccgc accttccaga
     3181 tcgtcaagcc tttcgccggc ctttcggtgc tcgacaacgt catggtgggt ggttttctaa
     3241 gagaaaaaaa tacctccggc gctcgcgcgc tctccatctc gatcctcgac cagctcggcc
     3301 tcggacccaa gcgcgacctg cccgcctcct cgctcaccct tcccgaccgc aagcggctgg
     3361 aggtcgcgcg cgcgctcgcc acgcggccgc ggctgttgct gctcgacgaa gtgatggcgg
     3421 gcctgcgccc gaccgagtgc gaccagatgg tcgaggtgtt ccgcgagctg aaccgcgctg
     3481 acggcctcac catcgtcctc atcgagcacg tgatgcgcgc cgtgatggcg ctcgcgcgcg
     3541 acatcgtcgt gctccaccac ggcgaagtca tcgcccgcgg cacgccggcc gaggtggtgc
     3601 gcgaccgggc ggtgctcgag tgctacctgg gcgaggaaac ggaaatatga ctgcgtttct
     3661 tcaaattgag aatctcaatc tgtactacgg cgatgcccag gcgctctccg acgtctcgct
     3721 tgaagtgccc aggggggaga tcgtcgccat cgtcggcgcc aacggcgccg gcaagtcctc
     3781 gttgatccgc accatcgccg gcatcgagaa gcgccgttcc ggaaagatcg ttttcaatga
     3841 agcggacatc tccgtcctgg aatcccacgc catctgcaac ctcggcatcg gccaggtcgc
     3901 cgaaggccgc caggtgttcc ccagcctcac cgtgttcgag aacctcgagg tgggcgccat
     3961 gctgccgcgc gcgcggcgca gcgcccgccg tttgctcgac caggtgttcg ccctgttccc
     4021 gcgcctgcgg gagcgcacgg cgcaggtcgc cggcaccctg tccggcggcg agcagcagat
     4081 gctcgccatc ggccgctgcc tgatgggcgc gcccgagctc gtcatgttcg acgagccctc
     4141 gctcggcctt gcgccgaccg tggtccaggc cgtgctcgcg accatccgcg acctcaaccg
     4201 ggatgggctg acctgcgttc tggtcgagca gaacgtcgcg gtgtcgctca ggctcgccaa
     4261 tcgcgcctac gtgctggaga acgggcgcat cacgctcgcc ggcagcggcg ccgagctctt
     4321 ggccgacgat cgcgtgcggc aggcctatct cggactgtga gccgcctcgt ctccggt
//
LOCUS       DASPXW010000103        11261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_283227, whole genome shotgun sequence.
ACCESSION   DASPXW010000103 DASPXW010000000
VERSION     DASPXW010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11261
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283227"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1209)
                     /gene="aceB"
                     /locus_tag="VEL04_02420"
     CDS             complement(<1..1209)
                     /gene="aceB"
                     /locus_tag="VEL04_02420"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017331735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HYT47253.1"
                     /translation="MQLPLARPQDASVNARVSAEFAEILTPEALAFVARLHRKFESSR
                     QELLSRRTARQQEFDAGKLPDFLPETSDIRKQEWAVAPQPRDLLERRVELTGPTDRKM
                     VINALNSGASTFMADFEDANCPTWHNMIDGQVNLRDAVRRTISFEQSGKRYRLNENTA
                     VLIPRPRGWHLDEKHVRVDGRAVSGSLFDFALFFFHNAKELMARGSGPYFYLPKMESR
                     LEARLWNDVFVFSQKELDVPRGSIKATALIETLLAAFEMDEILYELREHSAGLNIGRW
                     DYIFSCIKKFRANQDFCLADRSQVTMTAPFMRAYSLLLVKTCHRRGAPAMGGMAAQIP
                     IKNDAAANEAALEKVRQDKLREVTDGCDGTWVAHPGLVPVAKQVFDEHMRGPNQYGRQ
                     RPDVAVSAKHL"
     gene            1308..2417
                     /locus_tag="VEL04_02425"
     CDS             1308..2417
                     /locus_tag="VEL04_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47254.1"
                     /translation="MKSICKILILGASYGSLLATKLLLAGHTVKLVCLPAEAELINKE
                     GTRVRMPVKGRDGLVEVDSRKLPGKLSADIPGAIKPSDYDLVALAMQEPQYRSPGVRE
                     LLDAVAKAGVPCMSIMNMPPLPYLARIPGVSVDACRSCYTDAAVWDSFDPALMTLCSP
                     DPQAFRPPEEKINVLQVRLPTNFKSARFPSDAHTAMLRRLEADIEAARFDNVELPVKL
                     KVHDSVFVPLAKWAMLIAGNYRCITKDGIRPIKEAVHSDLEASRAVYNWVVRLCVLLG
                     AEEKDLVPFEKYAAAAQGLASPSSAARALFGGAPHIERVDRLVQTIAAQKGLRLDALD
                     EVVRLVDAKLDANRKAAQPSVATAGLSPAKTKKRA"
     gene            complement(2414..3814)
                     /gene="ntrC"
                     /locus_tag="VEL04_02430"
     CDS             complement(2414..3814)
                     /gene="ntrC"
                     /locus_tag="VEL04_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HYT47255.1"
                     /translation="MKPVWIVDDDRSIRWVIEKALSREGIAFSSFSTAQEALEALSGG
                     APEVLVSDIRMPGLSGLELLQAVKTRHPAVPVIVMTAYSDLDSAVAAFQGGAYEYLPK
                     PFDVDQAVELIRRALDESRKEAQAMEPLGETPEILGQAPAMQEVFRAIGRLSQSSATV
                     LITGESGTGKELVARALHRHSARAAKPFIPINTAAMPKDLLESELFGHERGSFTGAQQ
                     QRRGRFEQAEGGTLFLDEIGDMPAELQTRLLRVLSDGTFYRVGGHQQLKVNVRVIAAT
                     HQNLEERVRDGSFREDLYHRLNVIRLRLPSLHERPEDIPLLARHFLAASGKQLGVEPK
                     RFSEDALAHLSRLAFPGNVRQLENLCHWLTVMAAGQVIDTGDLPTEFRDQAAGAAPDW
                     LAALEQEVERRLARGDTGILDALGRQFERALIVRALARTAGRRIDAANLLGMGRNTIT
                     RKIQELGIEDDSAPES"
     gene            complement(3811..4845)
                     /gene="glnL"
                     /locus_tag="VEL04_02435"
     CDS             complement(3811..4845)
                     /gene="glnL"
                     /locus_tag="VEL04_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HYT47256.1"
                     /translation="MASLGPEFAGLELLPTAVVALDGTLVVRYANPAAENLLATGAKS
                     LLGQPFPQLFAEREELERALREARVTHWDYSAQHVSYIRTGREPMPLACVVTGIEAQG
                     LVLLVELRPIEQQLRLAREERMVFEQQSNRELIRNLAHEIKNPLGGLRGSAQLLEREL
                     DKPSLREYTQVIIKESDRLQALMDRMLTPHRAPQIEPVSVHEVLERVRSLVKAEFGAE
                     IERDYDPSLPEVLGDREQLIQAVLNIARNAAQAGGKQVTFKTRALRQVTILRQRYRLA
                     LELLVVDDGPGVPEEIQDRIFNPLVSGREGGTGLGLSLAQTFVRYHQGVIEFESRPGR
                     TVFRILLPLT"
     gene            complement(4846..5316)
                     /locus_tag="VEL04_02440"
     CDS             complement(4846..5316)
                     /locus_tag="VEL04_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47257.1"
                     /translation="MNWVRALIFVTFVLPAGPACAQVTEIYKCVEKSGRPLYTSDRRE
                     TRGKKCELVTREINVAPAQRPRAGNPSSFPKETAAERANAKGRQREILEKEFVAEQQA
                     LAKARQDLAEQESVRGGDERNFARVEQRLQPYKDSVELHEKNIEALRRELNNLR"
     gene            complement(5394..6809)
                     /gene="glnA"
                     /locus_tag="VEL04_02445"
     CDS             complement(5394..6809)
                     /gene="glnA"
                     /locus_tag="VEL04_02445"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HYT47258.1"
                     /translation="MAMTPDDVLKLIKDKEVKFVDLRFTDTRGKEQHVTVPVKYFGKD
                     KFELGHAFDGSSIAGWKGIQASDMLLMPDASSARMDPFTDEPVLNISCDVYEPMDGKP
                     YDRCPRSIAKRAEAYLKSTGLGDTSYFGPEPEFFIFDGITWSVEMSGCFVKIKSEEAP
                     WSSGIDYESGNMGHRAPIKGGYFPVPPTDTLLDIRNAMALAMEQQGVEVEVHHHEVGA
                     MGQNEIGTRFAPLVQRADWMQIYKYTVWNVAHSYGKTATFMPKPVVGDNGSGMHVHQS
                     VWKGGKNLFAGNGYAGLSEFALYYIGGIIKHAKALNAITNPGTNSYKRLVPGFEAPIN
                     LAYSARNRSAACRIPYVPSPNSRRVEVRFPDPTTNAYLAFAAMLMAGLDGVQSKTHPG
                     DPIDKDMYHLPPEEAARVPHVCASLEEALGCLHEDHAFLTKGGVFSEDFLESYVTLKE
                     DELTRFRMTTHPVEFDMYYSS"
     gene            6977..7417
                     /locus_tag="VEL04_02450"
     CDS             6977..7417
                     /locus_tag="VEL04_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HYT47259.1"
                     /translation="MDEIKKSAAERARRMGLNYAGALLPAEAHALMQAGGAKLVDVRT
                     RPELQFVGRIPGSIALEWQTYPGNRPNPEFLGELAALAPKETPLMFICRSGARSHGAA
                     EAATQAGWRECYNVLEGFEGDKDQNEHRNSVAGWRKAGLPWVQS"
     gene            complement(7419..8207)
                     /locus_tag="VEL04_02455"
     CDS             complement(7419..8207)
                     /locus_tag="VEL04_02455"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HYT47260.1"
                     /translation="MTDLFTALILGIVEGLTEFLPISSTGHLILAADLLGASGERWQV
                     FNIVIQTGAMLAVAWEYRARLFRVDTKLWLNLIVAFVPAAVLGLAFGSFIKSHLFHAV
                     PVAIAFIVGALIIFFVERKKLASRVESTQAMTWRDALKVGIAQCFALIPGTSRSGASI
                     IGGMLFGLSRPAATEFSFFLAVPTLIAAGGYDLLKHRALFSAQDLAPLGVGLVVAFAS
                     AFIVIRWLIRYVATHDFRPFAWYRIVFGLLVLLTAYFGWVDWKD"
     gene            complement(8200..8982)
                     /locus_tag="VEL04_02460"
     CDS             complement(8200..8982)
                     /locus_tag="VEL04_02460"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYT47261.1"
                     /translation="MRILATLAVGLPLVMYLLQERLIFYPQPLSDARREALAQRSPNV
                     ESVFVQASDGTRLHAWRVKAAPGAPLVLYFGGNAEEVSWMLDEIGRRAPAVGWLLTDY
                     RGYGASAGSPSEAALVSDALTWYDFSDKKLQTKSVYVFGRSLGSGVAVQLAAERPVAG
                     VILVAPFDSLVEVGKRHYPYLPVSWMLKHRFDSVSRAPGVRAPLLCLVATRDRIIPAA
                     HAKILYDAWGGPKRWVALEGADHNSTDDHPDYWPSITAFLRD"
     gene            9102..10271
                     /locus_tag="VEL04_02465"
     CDS             9102..10271
                     /locus_tag="VEL04_02465"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HYT47262.1"
                     /translation="MSELRFGMYIAELDRPWTDTPFRFQGFVLSTEQQLEALQKHCKT
                     VFVDLERSESPDAPRAAPLPLGRTLYPEKATVEQELGPARVAYTGSQLLMNAALSAVR
                     IGRTLDAERVRAAIDSLTASVLRNPDAMLLFSQLREKGEYAVSHALDVSIYMTAFGRF
                     LQLEPGQIEFLGYVGLLQDIGKVRLPKPLLEKRGRLNAEEFEQAKLHVNYSVEILRAT
                     PGIPRDLPRIAVLHHERQDGSGYPNHLRGSQIGMIGSIAGIIDTFDAMTVRRPHADPV
                     APSAAISNLYKWRGAFFDTQLVEQFIRFIGIFPVGSLVELNSGEIAVVMSQNPAKRLQ
                     PRVMVIRDSKGNPLRPQKLLDLSRSPKATPEEPYRILRTLEHGHVPVTSDELFMQ"
     gene            complement(10261..>11261)
                     /gene="thiC"
                     /locus_tag="VEL04_02470"
     CDS             complement(10261..>11261)
                     /gene="thiC"
                     /locus_tag="VEL04_02470"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase"
                     /protein_id="HYT47263.1"
                     /translation="TVPVYQALELAKDTKRLTAADLIDILEHQAKQGVDYFTIHAGVR
                     LPFIPLTAKRMTGIVSRGGSILAKWCLAHHKESFLYTHFEDICEIMAAYDVSFSLGDG
                     LRPGSIYDANDEAQLAELRTLGELTKKAWARDCQVMIEGPGHVPMQLIKENMELQLKY
                     CDEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPNKKDVK
                     DGIMAYKIAAHAADLAKGHPGAQLRDNALSKARFEFRWADQFNLGLDPDTAREFHDET
                     LPQDGAKVAHFCSMCGPHFCSMKITQDVRDYVEQGLKQKAVEFVKKGGELYQKPTA"
BASE COUNT         1956 a         3661 c         3669 g         1975 t
ORIGIN      
        1 gagatgcttc gcgctcacgg cgacgtcggg ccgctggcgg ccgtactggt tgggcccgcg
       61 catgtgctca tcgaacacct gcttcgcgac cggcaccagg cccgggtgcg ccacccaggt
      121 gccatcgcag ccgtcggtga cctcgcgcag cttgtcctgg cgcaccttct ccagcgccgc
      181 ctcgttcgcc gccgcatcgt tcttgatcgg gatctgcgct gccatgccgc ccatcgccgg
      241 cgcgccgcgc ctgtggcagg tcttgacgag cagcagcgag taggcgcgca tgaacggcgc
      301 ggtcatggtg acctgcgagc ggtcggcgag gcagaaatcc tgattggcgc ggaatttctt
      361 gatgcaggaa aagatgtagt cccagcgtcc gatgttgagt ccggccgaat gctcgcgcag
      421 ctcatacagg atctcgtcca tctcgaaggc cgcgagcaga gtctcgatca gcgcagttgc
      481 cttgatgctt ccccggggaa cgtccagttc tttctgggaa aaaacgaaaa catcgttcca
      541 cagccgcgcc tcgaggcgcg attccatctt cggcaggtag aaatacggcc ccgagccgcg
      601 cgccatcagc tctttggcgt tgtggaagaa gaacagcgcg aaatcgaaca gcgagcccga
      661 caccgcccgg ccatcgaccc gaacgtgctt ttcgtccagg tgccagccgc gcggccgcgg
      721 gatcagcacc gcagtattct cgttcagccg atagcgcttg ccgctctgct cgaaactgat
      781 ggtgcggcgc acggcgtcgc gcaggttcac ctgcccgtcg atcatgttgt gccaggtcgg
      841 gcagttcgcg tcctcgaaat cggccatgaa ggtcgatgcg cccgagttga gagcattgat
      901 caccatcttg cggtcggtcg ggccggtaag ctcgacgcgc cgctcgagca ggtctctcgg
      961 ctgcggcgcc accgcccatt cctgctttct gatgtcgctg gtctcgggaa gaaaatccgg
     1021 cagcttgccg gcgtcgaact cctgctggcg cgcggtgcgg cgcgagagca gctcctggcg
     1081 cgaggactcg aactttcggt gcagcctggc gacgaaagcc agcgcctccg gggtcaggat
     1141 ctcggcgaat tcagccgaga cgcgagcgtt gacggaggcg tcctggggac gcgcgagagg
     1201 caactgcatc ggcccattct atttgcggaa atgccgcact gcaacatgaa atcgcggacc
     1261 gaacggagta ggctcgcttt ccgaccagcg gggctgtatt tcacatcatg aaatccatct
     1321 gcaagattct aatcctgggc gcctcttacg gctccctctt ggcgaccaag ctcctgctcg
     1381 cggggcacac ggtcaagctc gtatgcctcc ctgccgaggc cgagctgatc aacaaggaag
     1441 gtacgcgggt gcgcatgcct gtcaaaggcc gtgacgggct ggtcgaggtc gactcgcgca
     1501 agctgccggg caagctttcc gccgacattc caggcgcgat caagccctct gactacgacc
     1561 tggtggcgct ggcgatgcaa gagccccagt atcgttcgcc cggcgtacgc gagctgctcg
     1621 acgcggtggc aaaggccggg gtgccttgca tgtcgatcat gaacatgccg ccgttgccgt
     1681 acctcgcgcg gattccgggc gtgtcggtcg atgcgtgcag gagctgctac accgatgccg
     1741 ctgtctggga cagtttcgac ccggcgctga tgacactgtg cagcccggac ccgcaggctt
     1801 tccgcccgcc cgaagaaaaa atcaacgtgc tgcaggtcag gctgccgacc aacttcaaat
     1861 cggcgcgctt tccgtcggac gcacataccg caatgctgcg tcgcctcgag gctgacatcg
     1921 aagcggcgcg cttcgacaac gtcgagctgc cggtaaagct caaggtgcac gactcagtct
     1981 tcgtgccgct cgcgaagtgg gcgatgctga tcgcgggcaa ttaccgctgc atcacaaagg
     2041 acggcatacg gcccatcaag gaagccgtgc acagcgatct cgaagcatca cgtgccgtgt
     2101 acaactgggt cgtcaggctg tgcgtgctgc ttggcgctga ggagaaggac ctggttccct
     2161 tcgagaagta tgccgcagcg gcgcaggggc tggcgagccc ttcttcggcg gctcgcgccc
     2221 tgttcggcgg cgcgccccat atcgagcgtg tcgatcgcct ggtccagacc atcgcggcac
     2281 agaagggcct gcgcctggac gcgctggacg aggtggtgcg tctcgtcgat gcgaagctcg
     2341 atgccaatcg caaggcggcg cagccgtccg tcgccacagc cggattgtct ccggccaaga
     2401 caaagaaaag agcctagctc tccggcgcgg aatcgtcttc gattccgagc tcctggattt
     2461 tcctggtgat ggtgttgcgg cccatgccga ggaggttggc ggcgtcgatg cgccggccag
     2521 cggtccgggc tagcgcccgg acgataagcg cgcgctcgaa ttggcgcccc agggcatcga
     2581 gaattccggt gtcgccgcgc gccaggcgcc gctcgacctc ctgctcgagc gcagcgagcc
     2641 agtcgggcgc cgcgcccgcc gcctgatcgc ggaattcggt cggcaggtcg ccggtgtcga
     2701 tcacctgccc ggcggccatc acggtgagcc agtggcacag gttctcgagc tggcgcacgt
     2761 tgccggggaa cgcaaggcgc gagagatgcg ccagcgcatc ctcggagaag cgcttgggct
     2821 cgacgccgag ctgcttgccg ctcgcagcga ggaaatggcg cgcgagcagc gggatgtcct
     2881 cggggcgctc gtgcaggctg ggcagccgca ggcggatcac gttcaggcgg tggtacaaat
     2941 cctcgcggaa cgagccgtcg cgcacgcgct cctcgaggtt ctggtgcgtg gccgcgatga
     3001 cgcgcacgtt caccttgagc tgctggtggc cgccgacgcg atagaaagtg ccgtcggaaa
     3061 gcactcgcag cagacgcgtc tgcagctcgg cgggcatgtc gccgatctcg tcgaggaaca
     3121 gcgtcccgcc ctcggcctgc tcgaagcggc cgcgccgctg ctgctgcgcg ccggtgaacg
     3181 agccgcgctc atggccgaag agctcggatt cgaggagatc cttcggcatc gcggcagtgt
     3241 tgattgggat gaacggcttc gcggcgcgtg cactgtggcg gtgcaacgca cgcgccacga
     3301 gctcctttcc ggttcctgat tcccccgtga tcagcaccgt cgcgctcgac tgcgacagcc
     3361 gcccgatcgc gcggaatacc tcttgcatgg caggtgcctg ccccaggatc tcaggcgtct
     3421 cgcccaaggg ctccattgct tgagcttcct ttctactttc gtcgagcgcg cggcgaatca
     3481 gctccaccgc ctggtccacg tcgaaaggtt tgggcaggta ctcgtaggca ccgccctgga
     3541 aagcggcgac cgcggaatcg agatcggagt aagcggtcat gacgatcacc ggcaccgccg
     3601 gatgtcgcgt cttgactgcc tgcagcagct cgaggcccga gagtcccggc atgcggatat
     3661 ccgacaccag tacctccggg gcgccgcccg agagcgcctc gagcgcctcc tgggcggtgg
     3721 agaacgagct gaaggcaatg ccttcccggc tgagcgcctt ctcgatcacc cagcggatgg
     3781 agcggtcgtc gtcgacgatc cagaccggtt tcatgtcagc ggcagcagaa tcctgaatac
     3841 ggtgcggccc ggccgcgact cgaactcgat cacgccctgg tggtagcgga cgaaagtctg
     3901 cgcgagcgag agccccaagc ctgttccgcc ttcccgtccc gaaacgagcg gattgaagat
     3961 ccgatcctgg atctcctccg gtacccccgg gccgtcgtcg actaccagca attctaatgc
     4021 cagcctgtag cgctgccgca ggatggtgac ctgcctcagt gctctggtct tgaatgtcac
     4081 ctgcttgccg cctgcctggg cggcattgcg ggcaatgttg agcaccgctt gaatcagctg
     4141 ctcgcgatcg ccgagcactt cgggcaggct cgggtcgtag tcgcgctcga tctcggcgcc
     4201 aaactctgcc ttcaccaggc tgcgcacccg ctcgagcacc tcgtggacgc tcaccggctc
     4261 gatttgcggc gcgcggtgcg gcgtgagcat gcggtccatc agcgcctgca ggcgatcgga
     4321 ttccttgatg atcacctgcg tgtactcgcg cagcgagggc ttgtcgagct cgcgctcgag
     4381 cagctgcgcc gagccgcgca ggccgccgag cgggttcttg atctcgtgcg cgagattgcg
     4441 gatcagctcg cggttcgact gctgctcgaa caccatgcgc tcttcgcgcg ccagccgcag
     4501 ctgctgctcg atcgggcgca gctcgacgag caacacgagc ccttgcgcct cgattcccgt
     4561 gaccacgcag gcgagcggca ttggctcgcg cccggtgcgg atgtagctga cgtgctgcgc
     4621 cgagtagtcc cagtgggtga cgcgcgcctc acgcagcgcg cgctcgagct cctcgcgctc
     4681 ggcgaagagc tgcgggaacg gctggccgag caggcttttc gcgccggtgg cgagcaggtt
     4741 ttccgccgct ggattggcgt agcgcaccac cagcgtccca tcgagcgcca cgacggccgt
     4801 gggcagcagt tcaaggccgg cgaattctgg cccgagggaa gccacttagc gcaggttgtt
     4861 gagctcgcgc ctgagcgctt ctatgttctt ctcgtggagt tctacgctgt ccttgtaggg
     4921 ctgcagccgc tgctccaccc gcgcaaagtt gcgctcgtcg ccgccgcgca ccgactcctg
     4981 ctcggcaagg tcctgtctcg ccttggcaag cgcctgctgc tcggcgacga actccttttc
     5041 gaggatttcg cgctggcgcc ccttggcgtt ggcgcgctcg gctgccgttt ccttgggaaa
     5101 gctggaggga ttgccggcgc gaggccgctg cgccggcgcc acgttgatct cgcgcgtcac
     5161 cagctcgcat ttcttgcccc gggtctcgcg cctgtcgctg gtgtagagcg gccggccgct
     5221 cttctcgacg catttgtaga tctcggtaac ctgggcgcag gccggccccg caggcaagac
     5281 gaaagtcacg aaaatcaagg ccctaaccca attcatgggg ccagtctacc caaaaaaaga
     5341 aaggggtgcg cgaagcacac cccggttccg gcctcaccga aaaggctgat ttcctaggag
     5401 gagtaataca tgtcgaactc gaccgggtgg gtggtcatgc gaaaccgcgt cagctcgtct
     5461 tccttgagcg tcacgtacga ctcgaggaaa tcctcggaga acacgccgcc cttggtgagg
     5521 aaggcatgat cttcgtgcaa gcagccgagc gcctcctcaa gcgatgcgca cacgtgcggt
     5581 acccgcgcgg cttcctcggg aggcaggtgg tacatgtcct tgtcgatcgg gtcaccgggg
     5641 tgggtcttgc tctgcacgcc gtcgaggccg gccatgagca tcgccgcgaa cgcgaggtag
     5701 gcattcgtgg tggggtcggg gaagcgcacc tcgacgcgtc gggagttcgg gctgggcacg
     5761 taaggaatgc ggcaggccgc cgagcggttg cgcgcggagt aggcgagatt gatcggcgcc
     5821 tcgaaaccgg gaaccagccg cttgtaggag ttggtgccgg gattggtgat ggcgttcagc
     5881 gccttcgcgt gcttgatgat gccgccgatg tagtacagcg cgaattccga cagcccggca
     5941 tagccgttgc ccgcgaacag gttcttgccg cccttccaca ccgactggtg cacgtgcatg
     6001 cctgagccgt tgtcgccgac taccggcttc ggcatgaaag tcgcggtctt gccgtaggaa
     6061 tgcgccacat tccataccgt gtacttgtag atctgcatcc agtcggcgcg ttgcaccagc
     6121 ggcgcgaagc gggtgccgat ctcgttctga cccattgcgc ccacttcgtg gtggtgcact
     6181 tcgacctcga cgccctgctg ctccatcgcg agcgccatgg cattgcggat gtcgagcagt
     6241 gtgtcggtcg gcggcaccgg gaagtaaccg cccttgatcg gcgcccggtg acccatgttg
     6301 cctgactcgt agtcgatccc tgaggaccag ggtgcctcct cggatttgat cttcacgaag
     6361 cagcccgaca tctcgacgct ccaggtaatg ccgtcgaaaa tgaagaactc gggctcgggc
     6421 ccaaaatagg atgtgtcgcc aaggccggtc gatttcaggt acgcctcggc gcgcttcgcg
     6481 atgctgcgcg ggcagcggtc gtagggcttg ccgtccatcg gctcgtagac gtcgcaactg
     6541 atgttgagca ccggctcgtc ggtgaacgga tccatgcggg ccgacgaggc gtccggcatg
     6601 agcagcatgt ccgaggcctg gatccccttc cagccggcga tcgacgagcc gtcgaacgcg
     6661 tgcccgagct cgaacttgtc cttgccgaag tacttcaccg gcaccgtgac gtgctgctcc
     6721 ttgccgcggg tgtcggtgaa cctgaggtcg acgaacttga cctccttgtc cttgatgagc
     6781 ttcaggacgt catcgggcgt catcgccatc gggaaaactc ctcgctgagt tggtgtagta
     6841 gtagtgagtc cgcggggcag cacattccat gccaaaacga accgcccaga aggcgggcat
     6901 tttacccaac tgatatgcac tgtatagggg catccggccg cattctgcac gacagcggtg
     6961 cttctataat tccgggatgg acgagatcaa aaagagcgct gcggaacgtg cccgcaggat
     7021 gggcctcaac tacgccggcg ccctgctgcc cgccgaggcg cacgcgctga tgcaggccgg
     7081 cggcgccaag ctggtcgacg tgcgaaccag gcccgagctg cagttcgtcg ggcgcattcc
     7141 aggctcgatc gccctggaat ggcaaacgta ccctggcaac cggccgaacc ccgagttcct
     7201 cggcgagctc gccgcgctcg cgcccaagga aaccccgctg atgttcatct gccgctcggg
     7261 ggcgcgctcg cacggcgcgg ccgaggccgc cacccaggcc ggctggcgcg agtgctacaa
     7321 cgtgctcgag ggattcgagg gcgacaagga ccagaacgag caccgcaaca gcgtcgccgg
     7381 ctggcgcaag gccggcctgc cctgggtgca aagctgattc agtccttcca gtcgacccag
     7441 ccgaagtagg cggtcagcag caccagcagc ccgaagacga tccggtacca ggcaaagggc
     7501 cggaagtcgt gcgtcgccac atagcggatc agccagcgga tcacgatgaa ggctgaagcg
     7561 aacgcgacca ccagtccgac gccgagcggc gcgagatcct gcgccgagaa cagcgcgcgg
     7621 tgcttcagca ggtcatagcc gcctgcggcg atgagcgtcg gcaccgcgag gaagaaggag
     7681 aactcggtcg ccgccgggcg cgacaggccg aacagcatgc cgccgatgat gctcgcgccc
     7741 gagcgcgacg tgcccggtat gagcgcgaag cattgcgcga tgccgacttt cagcgcgtcg
     7801 cgccaggtca tcgcctgggt gctttccacc cgcgacgcaa gtttttttct ttcgacaaaa
     7861 aaaatgatca gcgcgccgac gatgaaggcg atcgcaacgg gcacggcatg aaacagatgg
     7921 cttttgataa aagaaccaaa agcgaggccc agaactgccg ccggaacgaa ggcgacgatc
     7981 agattcagcc agagcttcgt atcgacgcga aacaagcgcg cccggtattc ccaggctacc
     8041 gcgagcatcg cgccggtctg gatcacgata ttgaagacct gccagcgctc gccgctggcg
     8101 ccgagcaagt cggcggcgag gatcaggtga ccggtgctcg agatcggcag gaactcggtc
     8161 agcccctcga cgatgccgag gatgagcgca gtgaacaggt cagtcacgca ggaaggccgt
     8221 gatgctgggc cagtaatccg ggtgatcgtc ggtgctgttg tggtccgcgc cttcgagcgc
     8281 gacccagcgc ttcggcccgc cccacgcgtc gtacagaatc ttcgcgtgcg cggcgggaat
     8341 gatccgatcg cgcgtagcga ccaggcacag gagcggcgcg cgcacgcccg gcgcgcgcga
     8401 caccgaatcg aagcggtgct tcagcatcca gctcaccggc aggtagggat agtggcgctt
     8461 tcccacttcc accaggctgt cgaacggcgc taccaggatc actccggcga ccggccgctc
     8521 ggccgcgagc tgcaccgcga cgccgctgcc gaggctgcgg ccgaagacat agacgctctt
     8581 ggtttgtaat ttcttgtcag aaaagtcata ccacgtgagg gcgtcggaga cgagggccgc
     8641 ctccgaaggc gagcctgcgc tcgcgccgta gccgcggtaa tcggtgagca gccagccaac
     8701 cgccggagcg cgccggccga tctcgtcgag catccaggag acttcctcgg cgttaccgcc
     8761 gaagtacagc acgagcggtg cgccaggcgc cgccttgacg cgccaggcgt gcagccgcgt
     8821 gccgtcggac gcctggacaa acacgctttc aacattgggg gagcgctgcg cgagcgcttc
     8881 ccggcgcgcg tccgaaaggg gctggggata aaagatcagg cgctcctgca gcaaatacat
     8941 gacaagcggc aggccgaccg ccagcgtcgc gaggatcctc aataccgagc tcaaagcctc
     9001 tgtctccact tcgccacccg cctgccgcca cgctatccct gcggccagcg gaagggctaa
     9061 tcttagggct ctagggaggt ggtttgaaaa aaagtatccc ggtgtccgag ctgcgcttcg
     9121 gcatgtacat cgccgagctc gatcggccgt ggaccgacac gccgttccgc tttcagggct
     9181 tcgtcctaag caccgagcag cagctcgagg cgctgcaaaa gcactgcaag accgtattcg
     9241 tcgacctgga gcgcagcgag tcgccggacg cgccgcgagc cgccccgctg cctctcggca
     9301 ggaccctcta tcccgagaag gcgacggtcg agcaggagct cggcccggcc cgtgtcgcct
     9361 acaccggcag ccagctgctg atgaatgcgg cgctgtcggc ggtgcgcatc gggcgcacgc
     9421 tcgatgccga gcgggtgcgc gcggccatcg acagcctgac cgcgagcgtg ctgcgcaacc
     9481 ctgacgcgat gctgctgttc tcccagctca gggaaaaagg cgaatacgcg gtgtcccatg
     9541 cgctcgacgt ctcgatctac atgaccgcct tcggccgctt cctgcagctc gagccgggcc
     9601 agatcgagtt tctcggctac gtcgggttgt tgcaggacat cggcaaggtg cgcctgccga
     9661 agccgctgct cgaaaaacgc ggccggctga atgccgagga attcgagcag gcgaagctgc
     9721 atgtcaacta ttcagtcgag atcctgcggg cgacgcccgg aattccgcgc gatctgccgc
     9781 gcatcgcggt gctgcaccac gagcggcaag acggcagcgg ctatccgaat catctgcgcg
     9841 gctcgcagat cggcatgatc ggatcgatcg caggcatcat cgacaccttc gacgcgatga
     9901 ccgtgcggcg tcctcacgcc gatcccgtcg cgccgtcggc cgccatcagc aatctgtaca
     9961 aatggcgcgg cgcgttcttc gacacccagc tggtcgagca gttcatccgc ttcatcggca
    10021 tcttcccggt gggcagcctg gtcgagctga acagcggcga gatcgcggtc gtcatgtcgc
    10081 agaaccccgc caagcggctg cagccgcggg tgatggtgat ccgcgactcc aagggcaatc
    10141 cgctgcggcc gcagaagctg ctggatctgt cgcgctcgcc caaggcgacg cccgaagagc
    10201 cttaccgcat cctgcgcacg ctcgagcacg gccacgtgcc ggtgacgagc gacgagctgt
    10261 tcatgcagta ggtttctggt acagctcgcc gcccttcttc acgaactcga ccgccttctg
    10321 cttcagcccc tgctcgacgt aatcgcgcac gtcctgcgtg atcttcatcg agcagaagtg
    10381 cggcccgcac atcgagcaga agtgcgccac cttggcgccg tcctgcggca gcgtctcgtc
    10441 atgaaattcc cgcgcagtat ccggatccag cccgaggttg aactggtcgg cccagcggaa
    10501 ctcgaagcgc gctttcgata gcgcgttgtc gcgcagctgc gccccgggat ggcccttggc
    10561 gaggtcggcg gcgtgcgcgg cgatcttgta agccatgatg ccgtccttca cgtccttctt
    10621 gttcggcagg ccgaggtgct ccttcggcgt cacgtagcac agcatcgcgg tgccgtacca
    10681 gccgatcatc gcggcgccga tggcgctcgt gatgtggtcg tagcccggcg cgatgtcggt
    10741 ggtgagcggc ccgagggtgt agaacggcgc ctcgtcgcag tatttcagct gcagctccat
    10801 gttctccttg ataagctgca tcggcacgtg gcccgggcct tcgatcatca cctggcagtc
    10861 gcgcgcccac gccttttttg tcaattcccc caaagtacgg agttcggcca gctgagcctc
    10921 atcgttggcg tcgtagatcg agcccggccg cagcccgtcg ccgagcgaga acgagacgtc
    10981 gtacgcggcc atgatctcgc agatatcctc gaagtgcgtg tagaggaagc tctccttgtg
    11041 gtgcgcgagg caccacttcg ccaggatcga gccgccgcgc gacacgatgc cggtcatgcg
    11101 cttcgcggtg agcggaatga agggcagacg cacgcccgcg tggatggtga agtagtcgac
    11161 gccctgcttg gcctggtgct cgaggatgtc gatgaggtcg gccgccgtca ggcgcttggt
    11221 gtccttggcc agctcaaggg cctggtagac gggcacggtg c
//
LOCUS       DASPXW010000104         3649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1167485, whole genome shotgun sequence.
ACCESSION   DASPXW010000104 DASPXW010000000
VERSION     DASPXW010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1167485"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VEL04_02475"
     CDS             complement(<1..506)
                     /locus_tag="VEL04_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HYT47264.1"
                     /translation="MFGFLRSYFSNDLAIDLGTANTLIYVRGKGIVLNEPSVVAIRQE
                     AGPNGKKTIQAVGKEAKMMLGKTPGNIVAIRPMKDGVIADFTVTEQMLKQFIKKVHDN
                     KLFSPSPRIIICVPCGSTQVERRAIRESAMGAGAGQVFLIEEPMAAAIGADLPVAEAT
                     GSMVVDVGG"
     gene            645..953
                     /gene="gatC"
                     /locus_tag="VEL04_02480"
     CDS             645..953
                     /gene="gatC"
                     /locus_tag="VEL04_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HYT47265.1"
                     /translation="MSLSHDEVLRIARLARIAVRPDDSRAVVERLNRVLALVDQMRAV
                     DTAGIEPMAHALDIVQRLRPDQVTEKDRREAYQAVAPAGPDGTPQVQEGFYLVPRVIE
                     "
     gene            957..2423
                     /gene="gatA"
                     /locus_tag="VEL04_02485"
     CDS             957..2423
                     /gene="gatA"
                     /locus_tag="VEL04_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018441276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HYT47266.1"
                     /translation="MLNSTLTELSAALAAKKISSVELTRLCLERIGRLNGELNAFIWP
                     EKTQDFALAQARRADERRACGDAGPLTGIPIAFKDIYCTKGIHTTCGSKMLETFVSPY
                     DAHVVEQFDRAGSVLLGKCNMDEFAMGSSNESSHFGPVHNPWDRRRVPGGSSGGSAAA
                     VAARLAPAATGTDTGGSVRQPAAFSGVCGLRPTYGLVSRYGLVAFASSLDQAGPIARS
                     AADLALLIGVMAGFDARDSTSVERPKEDYSKKLDLKLQGLRIGFPKEYFGDGIEPGVR
                     QAVDEAVRWFESQGARKVELAMPNTKLAVAVYYVIAPAEASSNLSRFDGVRYGHRAKQ
                     YKDLNDMYARTRDEGFGAEVKQRILVGTYVLSHGYYDAYYLQAQKLRRLIARDFAAAF
                     ERCDLVLGPTAPSTAFEIGANADDPVQMYLGDIFTVPQPLAGLPALSIPCGFDAKGLP
                     VGLQLTGNYFSEAALLGAAHRYQQATDWHLRVPTEAPL"
     gene            2420..3148
                     /locus_tag="VEL04_02490"
     CDS             2420..3148
                     /locus_tag="VEL04_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47267.1"
                     /translation="MRACALLFLAAFPVALFSQQQTETREMVGHIGSRSALLVLHATR
                     ATDGGWQLAGEYVLLPTLVRRFLEGEASPEIGVTTLKEGTTPILFGRSPTAELRGSLR
                     GGSFKGTRYGPGGQERERFEFSEEFPSMASYSAAVRCEAGDERYASSLSYTVDAGTLK
                     SLEWRSKVAPGGHSCMLAGAEQRPFKGGLRFVSGRCSVTLRDLGEYVRASVENCSELC
                     GSQAYLEPLLIRRGNCRLLRPETR"
     gene            3145..>3649
                     /gene="gatB"
                     /locus_tag="VEL04_02495"
     CDS             3145..>3649
                     /gene="gatB"
                     /locus_tag="VEL04_02495"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419089.1"
                     /note="allows the formation of correctly charged
                     Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation
                     of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms
                     which lack either or both of asparaginyl-tRNA or
                     glutaminyl-tRNA synthetases; reaction takes place in the
                     presence of glutamine and ATP through an activated
                     phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HYT47268.1"
                     /translation="MTRGWEIVVGIETHTQLLTRSKMFSGAANAFGAAPNTYASAVDI
                     ALPGTLPVANRAAVEHAIRFGLAAKGTISRRSIFARKNYFYPDLPKGYQISQYESPIV
                     QGGEVQIEGKTVRLTRAHLEEDAGKSLHEDFHGMTGIDLNRAGTPLLEIVTEPELRGA
                     KDAVAYAR"
BASE COUNT          588 a         1257 c         1209 g          595 t
ORIGIN      
        1 ccgccgacgt ccaccaccat cgagcccgtg gcttccgcga ccggcaggtc cgcgccgatc
       61 gccgcggcca tcggctcctc gatcaggaac acctggcccg cgcccgcgcc catcgcgctt
      121 tcccttattg cgcggcgctc gacctgggtc gagccgcagg gcacgcagat gatgatgcgc
      181 gggctgggcg agaacagctt gttgtcatgc actttcttga tgaactgctt cagcatctgc
      241 tcggtgacgg tgaagtctgc gatcaccccg tccttcatcg ggcggatggc gacgatgttg
      301 ccgggggttt tcccgagcat catcttggct tccttgccga ccgcctggat ggtcttcttg
      361 ccgttcggcc ctgcttcctg ccggatggcg acgacggagg gctcgttgag cactatccct
      421 ttgccgcgca cgtagatgag cgtgttggca gttccgaggt cgatcgcgag atcgtttgag
      481 aagtagctgc gaaggaatcc gaacatgagc aaggtaccaa ccctttttcg tttcttaacc
      541 gggccttagg gcgctccgcc caggccgttc aaagtggccg ctatgatacg ctaacgacgc
      601 ccggggagat aagggcattc tcccttggcc cccggtccga gcgaatgtcg ctatcccacg
      661 acgaagtgtt gcgtatcgcg cgcttggcgc gcatcgcggt tcgtcccgac gattcccggg
      721 cggtcgtcga gcgcctgaac cgggtcctcg ccctcgtcga ccagatgcgc gcggtcgaca
      781 ccgccggcat cgagccgatg gcgcacgcgc tggacattgt tcaacggtta cgccccgacc
      841 aggtgaccga aaaggaccgg cgggaggctt atcaggccgt cgccccggcg ggtcccgacg
      901 gaacaccgca agtgcaggag ggcttctacc tggtgccgag agtcatagag taaagcttgc
      961 tgaactcgac cctcacggag ctctcggcgg cgctcgcggc aaagaagatt tcctcggtcg
     1021 agctgacccg cctctgcctg gagcgcatcg gccgtctgaa cggcgagctg aatgccttca
     1081 tctggccgga gaagacgcaa gacttcgcgc tcgcgcaggc gcggcgcgcc gacgagcgcc
     1141 gcgcctgcgg cgacgccggc ccgctcaccg gcatcccgat cgccttcaag gacatctact
     1201 gcaccaaggg aatccacacc acctgtggct cgaagatgct ggaaaccttc gtcagtccct
     1261 acgacgctca cgtcgtcgag cagttcgacc gcgccggcag cgtgctcctc ggcaaatgca
     1321 acatggacga gttcgcgatg ggctcgtcga acgagagctc gcacttcggc ccggtgcaca
     1381 acccctggga ccggcgccgc gtcccaggcg gctcctccgg cggctcggcg gcggcggtgg
     1441 cggcgcggct ggcgccggcg gcaaccggca ccgacaccgg cggctcggtc cggcagccgg
     1501 cggcgttctc cggggtctgc ggcctgcggc cgacctacgg cctggtgtcg cgctacggcc
     1561 tggtcgcgtt cgcctcgtcg ctcgaccagg ccgggccgat cgcgcggagc gccgccgacc
     1621 tggcgctcct cataggcgtc atggccggct tcgacgcccg cgattccacc agcgtcgagc
     1681 gacccaagga agattattcg aagaagcttg atctgaagct gcaggggctg cggatcgggt
     1741 ttcccaagga gtattttggc gacggcatcg agcccggcgt gcgccaggcg gtggacgagg
     1801 cggtgcgctg gttcgaatcg cagggcgcgc gcaaggtcga gctggcgatg ccgaacacga
     1861 agctggcggt cgccgtgtac tacgtcatcg cgccggccga ggcgtcgtcc aacctgtcgc
     1921 gcttcgacgg cgtgcgctac ggccaccgcg cgaagcagta caaggatctc aacgacatgt
     1981 acgcgcgcac gcgcgacgag ggcttcggcg ccgaggtgaa gcagcgcatt ctggtcggca
     2041 cctacgtgct gtcgcacggc tactacgacg cctactatct gcaggcgcag aagctgcgcc
     2101 gcctcatcgc gcgcgacttc gccgcggcgt tcgagcgctg cgacctggtc ctcggcccga
     2161 cggcgccgag caccgcgttc gagatcggcg ccaacgccga tgacccggtg cagatgtacc
     2221 tcggcgacat cttcaccgtt ccgcagcccc tcgccggcct gccggccctg tcgattccct
     2281 gcggcttcga cgcaaaaggc ctgccggtag gtctgcagct gaccggcaac tatttctccg
     2341 aggcggcgct gctcggcgcg gcgcaccgct atcagcaagc gaccgactgg catctgcgcg
     2401 tgcccacgga ggcaccgcta tgagggcttg cgcactgtta ttcctcgcgg catttcccgt
     2461 tgcgctcttt tcgcagcagc agaccgagac gcgcgagatg gtgggacaca tcggcagccg
     2521 cagcgcgctg ctcgtattgc acgccacccg cgccacggac ggcggctggc agctcgccgg
     2581 cgagtacgtg ctgctgccga cgctcgtgcg ccgctttctc gagggcgagg cgagccccga
     2641 gatcggcgtc accacgctca aggaaggcac caccccgatc ctgttcgggc gctcgcccac
     2701 cgccgagctg cgcggcagcc tgcggggcgg cagcttcaag ggcacgcgct acgggccggg
     2761 ggggcaggag cgcgagcgct tcgagttcag cgaggagttc ccttcgatgg ccagctacag
     2821 cgcggcagtg cgctgcgagg cgggcgatga gcgctatgca tcgagcctga gctacacggt
     2881 ggacgccggc acgctcaagt cgctcgagtg gcgctcgaaa gtggcgccgg gcggccatag
     2941 ctgcatgctc gccggcgccg agcagcgccc cttcaaaggc ggcctgcggt tcgtgtcggg
     3001 ccgctgcagc gtgacgctgc gcgacctggg cgagtacgtg cgcgcgtcgg tggaaaactg
     3061 cagcgagctg tgcggctcgc aggcctatct cgagccgctg ctcatccggc gcggcaactg
     3121 caggctgctg cggccggaga cgcgatgacg cgcggctggg aaatcgtcgt cggcatcgag
     3181 acgcacaccc agcttctcac gcgcagcaag atgttctcgg gcgccgccaa cgccttcggc
     3241 gccgccccga acacatacgc ctccgcggtc gacatcgcac tccccgggac gcttccggtc
     3301 gccaaccgcg ccgccgtcga gcacgcgatc cgcttcggcc tggcggcgaa gggaaccatc
     3361 agccgccgct cgatcttcgc cagaaagaat tacttctatc cggacctgcc caaggggtac
     3421 cagatcagcc aatacgagtc gccaatcgtg cagggtggcg aggtgcagat cgagggaaag
     3481 accgtccgcc ttacgcgcgc ccacctcgag gaagacgccg ggaagtctct ccacgaagat
     3541 tttcatggca tgaccggcat cgacttgaac cgcgcgggca cgccgctgct cgagatcgtc
     3601 accgagcccg agctgcgcgg cgccaaggac gcggtcgctt acgcgcgca
//
LOCUS       DASPXW010000105         5470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1167972, whole genome shotgun sequence.
ACCESSION   DASPXW010000105 DASPXW010000000
VERSION     DASPXW010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5470
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1167972"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1137)
                     /locus_tag="VEL04_02500"
     CDS             complement(<1..1137)
                     /locus_tag="VEL04_02500"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYT47269.1"
                     /translation="MKRIFYGWYMVAAGSGLQFLQAGLMTQAFGAYVAVLQEERGWSK
                     TALSGAAALQQMEVAILGPVLGWFIDRFGPQGVIRVGVLVFGCGLMLLSRTDSLPGFY
                     AAFIVIALGSSLCGFFPVNVALINWFERWRARALSSMSLGLALGGVSVPLVAWSLQAF
                     GWRATAFGSGVVAIAVGLPLAFVIRRRPEDHGETMDGISQQPPPGRTDLENATAEATR
                     DFTAREALRTPAFWLLALGHGFALFVVHAVTVHSITHMNQGLGYSVEQASLVYLLLTV
                     SQIGGVMLGWLIGDRFDKRLIAAACMLAHMTALLLLTYAVALPMVLAFALLHGAAWGL
                     RGPFMQALRADYFGRSAIGMILGLSFMIIVIGQIGGPMIAGILAD"
     gene            1156..1662
                     /locus_tag="VEL04_02505"
     CDS             1156..1662
                     /locus_tag="VEL04_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HYT47270.1"
                     /translation="MDISMYQASAPRFAGMLRNLSALLDNGQAYCDAKKIEPLVITGA
                     RLFPDMYPLTRQVQIACDTAKGAVARLAAIEVPKHEDTEQTFAELKGRIAKTLDFIES
                     VKAEKIDGAEDREIVLKMRGGERKFSGLQYLLGFAYPNFYFHVTTAYNILRHNGVEIG
                     KTDFIGKS"
     gene            complement(1757..2056)
                     /locus_tag="VEL04_02510"
     CDS             complement(1757..2056)
                     /locus_tag="VEL04_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47271.1"
                     /translation="MSAAAKEGSKPSGKEPYVELAEKIFIGLATRVYGTLAGSEQKKP
                     DPKALAVFSFKLADAFEQATKETDRAVAAAEIANKAKVTLADVDLSSVFATTKKP"
     gene            complement(2053..3840)
                     /locus_tag="VEL04_02515"
     CDS             complement(2053..3840)
                     /locus_tag="VEL04_02515"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HYT47272.1"
                     /translation="MEHGNLFLIQASVYFAAAVLAVMLAHQLGLGSVAGYLLAGIAIG
                     RWGFGLVEQVEDVRAFAELGVVFLLFLIGLELEPNRLWSMRSRLIGLGLSQVLGSIAA
                     IVAVAWALGVDLRVALVAGMALSFSSTALALQPLTERGGLGTQGGQATFAILLFQDLA
                     VIPMLAVLPLLGAAGGRSGFSWLALLYAVAAIVATLVLGHYLARPVFRHIARTRLREV
                     FTAFALLLVLAIALVFEAAGLSMALGAFLAGVLLADSEYRHEIEAAIDPFKGLLLGLF
                     FISVGMSVDFAVLLAKPWIVVGLIAGLFIVKGAVLWFIARFARLPADQRPLFILLVAQ
                     GGEFAFVLLGLGAARGAIPAETAHAITLAVALSMLATPFMLLFHDRVLAPRLARAAEK
                     PADTPEAAKMIVAGLGRVGQVVARLLNASGYHPTVLDDDPDHVEQCRKFGFRVFYGDA
                     TRLDLLHAAGADSADFLIITLDEPETITRLARTAKANFPRLRIIARAHDMRHMFELRD
                     LGIEASERETWLSALRLGETALAEISADPERARRAARAFAEHDGEVLAKLYEVHRSAP
                     DAHVTVSNELRDQLARTLSEDERSNISVR"
     gene            3873..>5470
                     /locus_tag="VEL04_02520"
     CDS             3873..>5470
                     /locus_tag="VEL04_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HYT47273.1"
                     /translation="MKIDWDIPIRMDDGLELRADLFRPQAEGRCPAILSYGPYGKGLA
                     FQEGYKSAWEIMARDHPDALAGSSNQYQNWEVVDPERWVPEGYACIRVDSRGAGRSPG
                     YLCHNNARETRDIHLCIEWVAQQPWCSGRIGMNGISYYAANQWRAAASQPPHLAAVCA
                     WEGWNDAYRDGNRHGGIICTFRKHWQDMQVKTVQHGQGERGAKSRVTGELVCGPDTLA
                     EKELVENRENMWGEILAREMDGPYYRERSADFSKITVPLLSAANWGGQGLHTRGNFEG
                     FVRASSRNKWLEVHGGSHWAPFYTDYGVRLQKRFFDFYLKSTPNGWDRQPRVQLNIRH
                     PGEKFVLRHEDEWPLARTQWTRFYLHKNLSLSEKPQKENGALSYDAMGDGVTFSMVAE
                     QQTEITGPSALKLLVSSSTTDADIFAVLRVFDRKGKEVLFQGALDPKTPVAQGWLRAS
                     HRKLDPQLSLPYRPWHSHQEKQPLEPGKPVELDVEIWPTCIVVPAGYRIALSIRGRDY
                     EHDGEAASLSNMKNPMKGCGPFVH"
BASE COUNT         1008 a         1817 c         1883 g          762 t
ORIGIN      
        1 gtcggcgagg atgccggcga tcatcggtcc gccgatctgc ccgatgacga tgatcatgaa
       61 cgacaggccg aggatcatgc cgatcgcgct gcggccgaag tagtcggcgc gcagcgcctg
      121 catgaacggg ccgcgcagcc cccaggcggc gccgtgcagc agcgcgaagg cgagcaccat
      181 gggaagcgcc accgcatagg tgagaagcag cagcgccgtc atgtgcgcca gcatgcaggc
      241 cgccgcgatc aggcgcttgt cgaagcggtc gccgatcagc cagccgagca tgacgccgcc
      301 gatctgcgac acggtgagca gcaggtagac gagcgaggcc tgctccacgc tgtatcccag
      361 cccctggttc atgtgggtga tcgagtgcac cgtgaccgca tgcaccacga acagcgcgaa
      421 gccgtggccg agcgcaagca gccagaaagc cggcgtgcgc agcgcctcgc gcgcggtgaa
      481 gtcgcgggtc gcctccgctg ttgcattctc aagatccgtt ctccccgggg gaggttgttg
      541 agagattcca tccatcgtct caccgtggtc ctcgggccgt ctgcggatca cgaaggcgag
      601 cggcaggccg acggcgatcg ccaccacgcc ggagccgaac gcggtggcgc gccagccgaa
      661 ggcctgcagc gaccaggcca ccagcggcac cgacacgccg ccgagcgcga gcccgagcga
      721 catcgacgac agcgcgcggg cgcgccagcg ctcgaaccag ttgatgagcg cgacattcac
      781 cgggaagaag ccgcacaggc tcgagccgag cgcgatgacg atgaaggcgg cgtagaagcc
      841 cggcagcgag tcggtgcggc tgaggagcat cagcccgcag ccgaacacca gcacgccgac
      901 gcggatcacg ccctgcggcc cgaagcggtc gatgaaccag ccgaggaccg ggccgaggat
      961 tgccacctcc atctgctgca gcgccgccgc gcccgagagc gcggtcttgc tccagccgcg
     1021 ctcctcctgc agcaccgcga cgtaggcgcc gaaagcctgg gtcatgaggc cggcctggag
     1081 gaactgcagc cccgagccgg ccgccaccat gtaccagccg tagaagatgc gcttcatgcc
     1141 ggtagaatgg ctgtcatgga catctcaatg tatcaggcga gcgcgccgcg cttcgccggc
     1201 atgctgcgca atctgtcggc gctcctcgac aatggccagg cgtattgcga tgcgaagaag
     1261 atcgagccgc tcgtgatcac cggcgcgcgc ctgttccccg acatgtaccc gctgacgcgc
     1321 caggtgcaga tcgcctgcga cacggcaaag ggcgcggtgg cgcggctggc ggcgatcgag
     1381 gtgcccaagc acgaggacac cgagcagacg tttgccgagc tgaagggccg catcgcaaaa
     1441 accctcgatt tcatcgagtc ggtgaaggcc gagaaaatcg acggcgccga ggaccgggag
     1501 atcgtgctca agatgcgcgg cggcgagcgc aagttcagcg gcctgcagta cctgctcggc
     1561 ttcgcctacc cgaacttcta cttccacgtc acgaccgcct acaacatcct gcgccacaac
     1621 ggcgtcgaga tcggcaagac ggacttcatc ggcaagtcct agccgtaccg cgagaggatc
     1681 gcggcggtct cgcgcggcag cgaaatttgc ggatcgtggt gcgtcggcag ctaggcgccg
     1741 tgcaccagga cgaaagtcag ggcttcttgg tcgtcgcgaa cacgctggag agatcgacgt
     1801 ccgcgagggt gaccttcgcc ttgttggcga tctcggcggc tgccacggcg cggtcggttt
     1861 ccttcgtcgc ctgctcgaac gcgtcggcga gcttgaagct gaacactgcg agggccttcg
     1921 ggtcgggctt tttctgctcg ctgccggcga gcgtgccgta gacccgcgtg gcgagtccga
     1981 tgaagatctt ctcggccagc tcgacgtagg gttccttgcc tgacggtttt gacccttcct
     2041 tggctgcagc gctcatcgga ccgagatgtt actacgctcg tcctcgctca gggtgcgggc
     2101 gagctggtcg cgcagctcgt tcgacaccgt gacgtgagca tcgggcgcgc tgcggtgcac
     2161 ctcgtagagc ttcgccagca cttcgccgtc gtgctcggcg aaggcgcgcg cggcgcggcg
     2221 cgcgcgctcg gggtcggcgc tgatctccgc gagggctgtc tcgccgagcc tgagcgccga
     2281 cagccaggtc tcgcgctcgc tcgcctcgat gccgaggtcg cgcagctcga acatgtggcg
     2341 catgtcatgc gcgcgcgcga tgatgcgcag ccgcgggaaa ttggccttcg cggtgcgcgc
     2401 caggcgggtg atcgtctcgg gctcgtcgag cgtgatgatc aggaagtccg cgctgtcggc
     2461 gcccgcggcg tgcagcaggt cgagccgcgt cgcgtcgccg tagaacaccc tgaagccgaa
     2521 cttgcggcac tgctcgacgt ggtcgggatc gtcgtcgagc acggtcgggt ggtagccgct
     2581 ggcgttcagc agccgcgcca ccacctggcc gacgcggccc aggccggcga cgatcatctt
     2641 ggccgcttca ggcgtatcgg ccggcttctc ggccgcgcgc gcgaggcgcg gcgccagcac
     2701 ccggtcgtgg aacagcagca tgaaaggggt ggcgagcatc gagagcgcga ccgcgagggt
     2761 gatggcgtgc gcggtctcgg cggggatcgc gccgcgcgcg gcgccaaggc cgagcagcac
     2821 gaaagcgaat tccccgccct gcgcgaccag caggatgaag agcggccgct ggtccgcggg
     2881 caggcgggcg aagcgcgcga tgaaccacag caccgcgcct ttcacgatga acagcccggc
     2941 gatcagcccg accacgatcc acggcttcgc cagcaggacg gcgaaatcga cgctcattcc
     3001 gaccgagatg aagaacaggc cgagcagcag ccccttgaag gggtcgatgg cggcttcgat
     3061 ctcgtggcgg tactccgagt cggcgagcag cacgccggcg aggaaggcgc ccagcgccat
     3121 cgacagaccg gcggcttcga acaccagcgc gatcgccagc acgagcagca gcgcgaaggc
     3181 ggtgaagacc tcgcgcagcc gggtgcgcgc gatgtggcgg aacaccggcc gcgccaggta
     3241 gtggccgagg accagggtgg cgacgatcgc cgccacggcg tagagcagcg cgagccagga
     3301 aaacccggag cgcccgcccg cggcgccgag cagcggcagc acggcgagca tcgggatcac
     3361 cgccaggtcc tggaacagca ggatggcgaa ggtcgcctgg ccgccctgcg tgccgagccc
     3421 gccgcgctcg gtgagcggct gcagcgcgag cgcggtcgag gagaacgaga gcgccatgcc
     3481 ggcgaccagc gcgacgcgca ggtcgacgcc gagcgcccag gccaccgcca cgatcgccgc
     3541 gatcgaaccc agcacctgcg acaggcccag gccgatcagc cgcgagcgca tgctccagag
     3601 ccggttcggc tcgagctcga ggccgatgag gaacagcagg aacaccaccc cgagctcggc
     3661 aaacgcgcgc acgtcctcga cctgctcgac gagcccgaat ccccagcggc cgatggcgat
     3721 gcccgcgagc aggtagcccg cgaccgagcc caggccgagc tggtgggcga gcatcacggc
     3781 aagcaccgcc gccgcgaaat agacgcttgc ctggatcagg aagaggttgc cgtgctccat
     3841 tggcgaagta tagttgggcc gaaattcagc gcatgaaaat cgactgggac attcccatca
     3901 ggatggacga cggcctcgag ctgcgcgcgg acctgttccg cccgcaagcc gagggccgct
     3961 gccccgcgat cctcagctac gggccgtacg gcaaggggct cgcgttccag gagggctaca
     4021 agagcgcctg ggagatcatg gcgcgcgacc atcccgacgc gctcgccggc tcctcgaacc
     4081 agtaccagaa ctgggaagtg gtcgacccgg agaggtgggt gcccgagggc tacgcctgca
     4141 tccgcgtcga ttcgcgcggc gccggccgct cgccgggcta tctgtgccac aacaatgcgc
     4201 gcgagacgcg cgacatccac ctgtgcatcg aatgggtggc gcagcagccc tggtgcagcg
     4261 gcaggatcgg catgaacggc atctcgtact acgcagccaa ccagtggcgc gccgcggcct
     4321 cgcagccgcc gcacctcgcc gcggtgtgcg cctgggaagg ctggaacgac gcctaccgcg
     4381 acggcaaccg ccacggcggc atcatctgca ccttccgcaa gcactggcag gacatgcagg
     4441 tgaagacggt gcagcacgga cagggcgagc gcggcgcgaa aagccgcgtg accggcgagc
     4501 tggtgtgcgg gcccgacacg cttgccgaaa aagaactggt cgaaaatcgc gagaacatgt
     4561 ggggcgagat cctggctcgc gagatggacg ggccctatta ccgcgagcgc agcgcagact
     4621 tctcgaaaat caccgtgccg ctgctctcgg ccgccaactg gggcgggcag ggattgcaca
     4681 cgcgtggcaa tttcgagggc ttcgtgcgtg cttcatccag gaacaagtgg ctggaagtgc
     4741 acggcggctc gcactgggcg ccgttctaca ccgattacgg cgtgcggctg cagaagcgct
     4801 tttttgactt ttatttgaag agcacaccca acggctggga caggcagccg cgcgtacagc
     4861 tcaatatccg tcatcccggc gagaagttcg tgctgcggca cgaggacgaa tggccgctcg
     4921 cgcgcacgca gtggacgcgc ttttatcttc acaaaaatct ttccctcagt gaaaaaccgc
     4981 aaaaggaaaa tggcgcgctc agctacgacg caatgggcga cggcgtcacg ttctccatgg
     5041 tggcggagca gcaaaccgaa atcaccgggc cgtcggcgct gaagcttctg gtttcctctt
     5101 cgacgaccga tgcggacatt ttcgccgtgc tgcgcgtatt cgatcgcaag gggaaggagg
     5161 tgctcttcca gggggcgctc gacccgaaga cgccggttgc gcagggatgg ctgcgcgcct
     5221 cgcaccgcaa gctcgatccg cagctgagcc tgccttaccg cccctggcac agccaccagg
     5281 aaaagcaacc gctcgagccc ggcaagcccg tcgagctcga cgtcgagatc tggccgacct
     5341 gtatcgtcgt gcccgcgggc taccgcatcg cgctcagcat ccgcggcagg gattacgagc
     5401 acgacggcga ggcggcgagc ctttccaaca tgaaaaatcc gatgaagggc tgcgggccgt
     5461 tcgtgcacga
//
LOCUS       DASPXW010000106         4113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1798291, whole genome shotgun sequence.
ACCESSION   DASPXW010000106 DASPXW010000000
VERSION     DASPXW010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1798291"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2811
                     /locus_tag="VEL04_02525"
     CDS             <1..2811
                     /locus_tag="VEL04_02525"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HYT47274.1"
                     /translation="LAAKECDLKLILGCEILLEEKLKLVLLAADRRSYGAISALITTG
                     RRRSRKGAYSLSRSDVETLSGSGALLLWVPGEDHAPASWVKRHFAGNAWIAAELHCGP
                     NDHAKLDALRKLSLESGLPLVAAGDVHMHLRSRKPLQDVLTAIRLGRPVAQCGQALQP
                     NAERSLRLRMRLAQLYAADLLAESVKISARCRFSLDELRYEYPQELVPPGETPASWLR
                     RLTQEGLRWRFHEDIPRQVQELAGHELGLIAELGYEPFFLTVHDIVRFARGQGILCQG
                     RGSAANSVVCYALGITEVDPARMSMLFERFVSKERNEPPDIDVDFEHQRREEVIQYVY
                     RKYGRARAALAATVICYRPRSAVRDAGKALGLPLEEAARLAKSFTFWDPGIEEACSPV
                     LKMAAALVGFPRHLSQHVGGFVISRGPLAELVPVENAAMPERTVIQWDKDDLEALGLL
                     KVDVLALGMLSAIRRALEMVGMQMQGIPAEDPAVYAMIQKADTIGVFQIESRAQMSML
                     PRLRPATFYDLVIEVAIVRPGPIQGGMVHPYLRRRRGLEPVAYPSPEVEKVLERTLGV
                     PIFQEQVMQLAVVAAGFTPGEADRLRRSMAAWKKRGGLEHFELKLKSGMQRNGYSAEF
                     ADSIYRQILGFGEYGFPESHSASFALLVYVSSWLKRHHPAAFCAALLNSQPMGFYAPS
                     QLVQDAQRHGVEVRPPDVNASGWDCTLEDGALRLGLRMVGGLSETAGQRIASGRPYAS
                     VTELDLNRKDLRSLAAAGALQSLAGHRRLAHWAAAGAVRRAPLDAPALERLPALAPPR
                     EGEDIVADYASLGLTLGRHPLALLRKHLQGLHLITAEDLKFKHHGSAVRVAGLVTCRQ
                     RPDTASGVVFVTLEDETGCVNVVVWASLVDSQRRELLGARLLGVQGAIERDGDVVHVV
                     ARRLVDYSALLGPLAAPSRDFH"
     gene            2824..3342
                     /locus_tag="VEL04_02530"
     CDS             2824..3342
                     /locus_tag="VEL04_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47275.1"
                     /translation="MPTYADILGLIAARRSRRPARNLPFLDALFRRLAACGSSEAPRT
                     EERIWEVWMDHPHRAAAHALDLATRDIAARRYDIAETRLTRLIRSAPGFAEAWHKRAA
                     LYYLLGRDRECLDDIRRTLELEPRHFCAMLHFAEILLGAGAPAEARFAFRAALSLHPH
                     QPRARQALSDKR"
     gene            3377..3682
                     /locus_tag="VEL04_02535"
     CDS             3377..3682
                     /locus_tag="VEL04_02535"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HYT47276.1"
                     /translation="MNHDPYRVIVARDLEDLIPVFMSNRRKELDALRVALAAADFEQL
                     RQLGHRMKGVGNSYGFARVSDLGKHVEDGARSGDRASLEAIITSYGEYLAKVQVAYE"
     gene            3675..3917
                     /locus_tag="VEL04_02540"
     CDS             3675..3917
                     /locus_tag="VEL04_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47277.1"
                     /translation="MSDLAAAAGRLAPAQAAFRVLVVDDDPDMAVFLARLLEGEGMIA
                     DTVFGGDAALVYVMATPPDLVLLGKAGETSIYSIKL"
     gene            3921..>4113
                     /locus_tag="VEL04_02545"
     CDS             3921..>4113
                     /locus_tag="VEL04_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47278.1"
                     /translation="MRVLLIDDDPDLRALLAHYIRQRWEDAKVEEYDPLSRELPDEAF
                     PLGSYDVVILDYMLGRGDGL"
BASE COUNT          646 a         1378 c         1403 g          686 t
ORIGIN      
        1 ctcgccgcta aggaatgcga tttgaagctg attctcgggt gcgaaatcct cctcgaggag
       61 aagctgaaac tcgtgctgct tgccgccgac cgccgctcct acggcgcgat ctcggcgctg
      121 atcaccaccg ggcgacgccg cagtagaaaa ggcgcctatt ctctttctcg ttctgatgtc
      181 gaaacgcttt cgggaagcgg cgcgctgctt ctgtgggtgc cgggggaaga ccacgccccg
      241 gcgagctggg tcaaaaggca tttcgcgggc aatgcctgga tcgcggccga gctgcattgc
      301 gggccaaacg atcacgccaa gctcgatgct ttgagaaagc tttcgctgga aagcggcctg
      361 ccgctagtcg ccgcgggcga cgtgcacatg cacctgcgct cgcgcaagcc actgcaggac
      421 gtgctgacgg cgatccggct cgggcggccc gtcgcgcaat gcggacaggc actgcagccg
      481 aacgccgagc gcagcctgcg attgcgcatg cggctggcgc agctttatgc cgcggatttg
      541 ctggcggaga gcgtgaagat ctccgcgcgc tgcaggtttt cgctcgacga gctgcgctac
      601 gagtatccgc aggagctggt gccgccgggg gagacgccgg cgagctggct gcgccggctc
      661 acgcaggagg gattgcgctg gaggttccat gaggacatcc cgcggcaggt gcaggagctc
      721 gccgggcacg agctcgggct gatcgccgag ctcggttacg agccgttttt cctcacggtg
      781 cacgacatcg tgcgcttcgc gcgcgggcag ggcatcctct gccagggccg cggctcggcc
      841 gccaactcgg tggtctgcta cgcgctcggc atcaccgaag tcgaccctgc caggatgagc
      901 atgctgttcg agcgcttcgt gtcgaaggag cgcaacgagc cgcccgacat cgacgtcgac
      961 ttcgagcacc agcggcgcga ggaagtgatc cagtacgtct accgcaagta cggccgcgcg
     1021 cgtgccgcgc tagccgcgac ggtgatctgc taccggccga ggagcgcggt gcgcgatgcc
     1081 ggaaaagcgc tcggcctgcc cctcgaggag gccgcgcggc tggcgaagag cttcacgttc
     1141 tgggacccgg ggatcgagga ggcctgcagc ccggtgctga agatggcggc ggccctggta
     1201 ggctttcccc ggcacctttc gcagcacgtc ggcggtttcg tcatctcgcg cgggccgctc
     1261 gccgagctgg tgccggtcga gaacgcggcg atgcccgagc gcaccgtgat ccagtgggac
     1321 aaggacgacc tcgaggcgct agggctcctg aaagtcgatg tactggcgct cggcatgctt
     1381 tccgccatca gaagagccct ggaaatggtt ggaatgcaaa tgcagggcat cccggccgag
     1441 gaccccgcgg tctacgccat gatccagaag gctgacacca tcggcgtgtt ccagatcgaa
     1501 tcgcgcgcac agatgagcat gctgccgcgc ctgcggcccg cgaccttcta cgacctggtg
     1561 atcgaggtgg cgatcgtgcg cccggggccg atccagggcg gcatggtgca tccctacctg
     1621 cgccgccggc gcgggctcga gccggtcgcc tatcccagcc ccgaggtaga gaaagtcctg
     1681 gaaagaaccc tgggcgtgcc gatcttccag gagcaggtga tgcagctcgc cgtggtcgct
     1741 gccggtttca cgcctggcga ggccgaccgc ctgcgccgct ccatggcggc gtggaaaaag
     1801 cgcggcggcc tggagcattt cgagctgaag ctgaagagcg gaatgcagcg caacggctac
     1861 agcgcggagt tcgccgactc gatctaccgc cagatcctcg gcttcggcga atacggcttt
     1921 ccggaatcgc actcggcaag cttcgcgctg ctggtgtacg tctcgtcctg gctcaagcgc
     1981 catcatccgg cggccttctg cgcggccctg ctcaacagcc agcccatggg tttttacgcg
     2041 ccttcccagc tggtccagga cgcgcagcgc cacggcgtgg aggtacgccc gcccgatgtc
     2101 aatgcgagcg gctgggactg cacgctcgag gacggcgcgc tgcgtctggg attgcgcatg
     2161 gtcggcggcc tgtcggagac ggctgggcag cggatcgctt cgggcagacc ttacgcttcg
     2221 gttacagaat tggacttgaa tcgaaaagat ctgcgctcgc tcgctgccgc cggcgcgctg
     2281 cagtctctcg ccggccaccg gcgcctcgcg cactgggccg cagccggggc cgtgcgccgc
     2341 gccccgctcg atgcgccggc gctcgagcgc ctgcccgcgc tcgcgccgcc gcgcgaaggc
     2401 gaggacatcg tcgccgatta cgcgagcctc ggcctgacgc tcggccggca cccgctggcg
     2461 ttgctgagaa agcatttgca gggtttgcat ctcattactg cagaagattt gaaattcaag
     2521 catcatggaa gcgcggtccg cgtggccggg ctcgtcacct gccggcagcg ccccgacacg
     2581 gcgagcggcg tggtgttcgt gaccctcgag gacgaaaccg gctgcgtcaa cgtcgtggtc
     2641 tgggcaagcc tcgtcgacag ccagcgccgc gagctgctcg gcgcgcgcct gctcggcgtg
     2701 cagggcgcga tcgagcgcga cggcgacgtg gtgcacgtcg tcgcgcggcg ccttgtggac
     2761 tacagcgcgc tgctcggccc cctcgcagcg ccttcgcgcg atttccatta agatttccag
     2821 gcgatgccga cctacgccga cattctcggc ttgatcgcgg cgcggcgcag ccgccgcccg
     2881 gcgcgcaacc tgccgtttct cgatgcgctg ttccggcggc tcgctgcctg cggcagcagc
     2941 gaagccccgc gcaccgagga gcgcatctgg gaggtgtgga tggaccatcc gcatcgcgcc
     3001 gcggcgcatg cgctcgatct cgcgacgcga gacatcgcgg cccgccgcta cgatatcgcc
     3061 gagacgcgcc ttaccaggct gatccgcagc gcgcccggct tcgccgaggc ctggcataag
     3121 cgcgccgcgc tctattacct gctcggccgc gatcgcgaat gcctcgacga catccgccgc
     3181 acgctcgagc tcgagccgcg ccatttctgc gccatgctgc atttcgccga gatcctgctg
     3241 ggcgctggcg cgccggccga ggcccgcttc gcgtttcgcg ccgcgctgtc gctgcacccg
     3301 caccagccga gggcccgcca ggcgctgtct gacaaacgct gacgggctgc aaagcgccgc
     3361 cccctataat aaaaacatga atcacgatcc ataccgggtc atcgtggcga gggacctcga
     3421 ggacctgatc ccggtcttca tgagcaaccg caggaaggag ctcgacgccc tgcgcgtggc
     3481 gctcgcggcg gcggatttcg agcagctgcg ccagctcggg catcgcatga agggcgtggg
     3541 gaactcctac ggctttgcgc gcgtctcgga cctcggcaag cacgtcgagg acggcgcgcg
     3601 cagcggcgac cgtgcctcgc tggaggccat catcacgtca tacggtgagt acctggcgaa
     3661 ggttcaagtc gcctatgagt gacctcgccg ctgccgccgg gcgcctcgcg ccggcgcagg
     3721 cggcgttccg ggtgctggtg gtggatgacg atcccgacat ggcggtgttt ctcgcacggc
     3781 tgctcgaggg tgagggcatg atcgccgaca ccgtgttcgg cggcgatgcg gcgttggtat
     3841 atgtcatggc cacgccgccc gacctggtgc tgctcggcaa ggccggagaa acctcgatct
     3901 actcgatcaa gctttaaccg atgcgggttc tgctcatcga tgacgacccg gacctgcgcg
     3961 ccttgctcgc gcattacatc cggcagcgct gggaagacgc caaagtggag gagtacgacc
     4021 cgctctcgcg cgagctgccc gacgaggcct tcccgctcgg ctcctacgat gtggtgatcc
     4081 tcgattacat gctcggccgg ggcgacgggc tgg
//
LOCUS       DASPXW010000107         2652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_538020, whole genome shotgun sequence.
ACCESSION   DASPXW010000107 DASPXW010000000
VERSION     DASPXW010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538020"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VEL04_02550"
     CDS             <1..301
                     /locus_tag="VEL04_02550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HYT47279.1"
                     /translation="KRERHVLQTVSIDLEIGLPGEHVFASDRVADTIDYEQVALRIRA
                     LAASGHFRLVETFADRIAALLVKDFSAPWVKVSAAKIGILANAKFVGVTIERRKS"
     gene            complement(303..1181)
                     /gene="xerD"
                     /locus_tag="VEL04_02555"
     CDS             complement(303..1181)
                     /gene="xerD"
                     /locus_tag="VEL04_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HYT47280.1"
                     /translation="MSDAAARLLDAFCDALWLEDGLSRNTISAYRVDLEQLSSFVKKD
                     LSEIGEADLFAFLASRRGRASSAARRVSSLKRFFQYCMRERRIAADPTLKLDAPKRAP
                     RFPKTLSESDVEALLAAPEVAAALGLRDRAMLETLYATGLRVSELVAMKIFEADLAAG
                     VVRVLGKGSKERLVPLGEEAVHWLRRYIEVSRKDLLRKKASDALFVTARGAGMTRQAF
                     WHLVRRYGARTIPGKRLSPHVLRHAFATHLINHGADLRVVQMLLGHADISTTQIYTHV
                     ARERLKQLHAKHHPRG"
     gene            complement(1178..1630)
                     /locus_tag="VEL04_02560"
     CDS             complement(1178..1630)
                     /locus_tag="VEL04_02560"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HYT47281.1"
                     /translation="MKVGVSTRNDRVVEICYLPSSTAVIAPRNPLAERAARQLERYRA
                     DPDATFDLPLLIEGSPLQRAVWEAMCSIPRGQTRTYGDLAQQLGAEARAVGQACGDNR
                     LPIVIPCHRVVAANGIGGFAHATEGFLIETKRWLLRHETNPDEFELRP"
     gene            complement(1665..>2652)
                     /locus_tag="VEL04_02565"
     CDS             complement(1665..>2652)
                     /locus_tag="VEL04_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HYT47282.1"
                     /translation="DLFRLARTALERAGAHARMAQAAARHLVRAEEQGLPTHGISRVP
                     FYCSMLRKGRADGAASPALLAERGGACLIDNRDGLPYVSVEWAVEEVIQRARRNGIGF
                     AGVRNSAHVGVLGIHLLPVAQARLAGFAFTNSPAAIPPWGGKKALFGTNPVAAVFPRE
                     RQHPIVIDLALTTVVRGKIMMAMRRGEKIPEGWALDRHGKPTTDPKEAIERGSLFPIG
                     GAKGAMLALMFELICAALTGAAIGPEADSFFSDEGNRPRIGQAFIAIDPGALAGLDRY
                     LERVETVVATMQADPGVRLPGARRFASEKSARSQGIEVPDELLVQIEKLAAA"
BASE COUNT          425 a          901 c          896 g          430 t
ORIGIN      
        1 caagcgcgag cgccacgttc tccagacggt atccatcgac ctcgagatcg gcctgccggg
       61 cgagcacgtg ttcgcgagcg acagggtggc cgacaccatc gattacgagc aggtggcgct
      121 gcgcatccgc gctctcgcgg cgagcgggca tttccgcctc gtcgagacct tcgccgaccg
      181 catcgccgcg ctgctcgtca aggatttcag cgccccctgg gtgaaagtga gcgcggccaa
      241 gatcggcatc ctggcgaacg ccaagttcgt cggcgtcacc atcgagcgcc gcaagagctg
      301 agctagcccc ttgggtggtg cttggcgtgc agctgcttca ggcgctcgcg cgcgacgtgc
      361 gtgtagatct gcgtggtcga gatgtcggca tgcccgagca gcatctgcac cacgcgcagg
      421 tcggcgccgt ggttgatgag atgggtagca aaggcgtggc gcaggacatg cggtgaaagg
      481 cgcttgcccg ggatggtgcg cgcgccgtag cgccgcacca ggtgccaaaa cgcctgccgc
      541 gtcatgccgg cgccgcgcgc ggtgacgaaa agcgcgtcgc tggctttttt tctcagcaga
      601 tcctttcgtg aaacctcaat atatcttcgc agccagtgca ccgcctcttc gccaagcggc
      661 accaggcgct ccttggaccc cttgccgagc acgcgcacca cgcccgccgc gaggtcggct
      721 tcgaaaatct tcatcgccac cagctcggaa acgcgcagtc cggtggcgta cagcgtttcg
      781 agcatcgcgc ggtcgcgcag cccgagcgcg gctgccacct ccggcgctgc gagcagcgcc
      841 tcgacgtccg actcggatag cgttttcgga aagcgcggcg cgcgcttggg cgcatcgagc
      901 ttcagcgtcg ggtcggcagc gatgcgccgc tcgcgcatgc agtactggaa gaagcgcttg
      961 aggctcgaca cccgccgcgc cgcgctcgag gcgcggcccc tgcgcgaggc gaggaacgcg
     1021 aacaagtccg cttcgccgat ctctgaaaga tctttcttta caaaactgga aagctgctcg
     1081 agatccaccc ggtaggcgct gattgtgttc ctcgacagcc cgtcctcgag ccagagcgcg
     1141 tcgcagaacg cgtcgagcag ccgcgcggcg gcgtcgctca gggccgcagc tcgaactcgt
     1201 cgggattcgt ctcgtgccgc agcagccaac gcttggtctc gatcaaaaag ccttcggtcg
     1261 catgcgcgaa gccgccgatg ccattggcgg cgacgacgcg atggcaagga atgacgattg
     1321 gcagccggtt gtcgccgcag gcctggccga cggcgcgcgc ttcagcgccg agctgctgcg
     1381 cgagatctcc gtaagtgcga gtctggccgc gcgggatcga gcacatcgct tcccatacgg
     1441 cgcgctggag cggcgagcct tcgatcagca acggcagatc gaacgtggcg tcaggatcgg
     1501 cgcggtatcg ctcgagctgg cgcgcggcgc gttcggcgag cgggttgcgc ggcgcgatta
     1561 cggcagtgga agaaggcaag tagcagatct cgaccacgcg atcgtttcgc gtgctcacgc
     1621 cgaccttcat gatggggaat tcgataacgg cgtcccagcg catgtcaggc tgccgccagc
     1681 ttctctatct gcacgagcag ttcgtccggg acctctatgc cttgcgagcg cgcgctcttt
     1741 tcgcttgcga agcgccgggc gcccggcagg cgcaccccgg gatcggcctg catcgtcgcc
     1801 acgacggttt ccaccctctc cagatatctg tccaggccgg cgagcgcgcc gggatcgatg
     1861 gcgatgaacg cctggccgat gcgcggccgg ttgccctcgt cggaaaagaa cgaatcggcc
     1921 tcgggcccga tcgcggcgcc ggtgagcgcg gcgcagatca gctcgaacat cagggcgagc
     1981 atcgcgcctt tcgcgccgcc gatggggaac aggctgccgc gctcgatcgc ctccttcggg
     2041 tcggtggtcg gcttgccatg ccggtcgagc gcccagccct cgggtatctt ctcgccgcgg
     2101 cgcatcgcca tcatgatctt gccgcgcacc accgtcgtca gcgccaggtc gatgacgatc
     2161 ggatgttgtc tttcgcgagg gaaaactgcc gcgaccgggt tggtgccgaa gagcgctttc
     2221 ttcccgcccc agggcgggat cgccgccggc gaattggtga acgcgaagcc ggcgagccgc
     2281 gcctgcgcga cgggaagcag gtggatgccg agcacgccga cgtgagcgct gttgcgcacc
     2341 ccggcgaagc cgatgccatt gcgccgcgcg cgctggatca cctcctcgac cgcccactcc
     2401 accgacacgt aaggcagccc gtcccggttg tcgatcaggc aggcgccacc ccgctccgcg
     2461 agcagcgcgg ggcttgccgc gccgtcggcg cgccctttgc gcagcatcga gcagtaaaaa
     2521 ggcacgcgcg atatgccgtg cgtgggaagc ccctgctcct cggcccgcac caggtggcgg
     2581 gcggcggctt gcgccatgcg cgcgtgggcg cccgcgcgct caagcgcagt gcgcgcgagt
     2641 cgaaacaggt ct
//
LOCUS       DASPXW010000108         7570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_914379, whole genome shotgun sequence.
ACCESSION   DASPXW010000108 DASPXW010000000
VERSION     DASPXW010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7570
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914379"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..573
                     /locus_tag="VEL04_02570"
     CDS             76..573
                     /locus_tag="VEL04_02570"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HYT47283.1"
                     /translation="MSHNGQPRVYLVAAVAANGTIGAAGQLPWRLPEDLTHFKRVTMG
                     HPVIMGRRTWESLGRPLPGRENIVVSTRRGYEAPGAAVASSLDAALALCAGEPVAFVI
                     GGTRLFEAALPLASGLVMTEIKRDYAGDAWFPQYDRSQWRESQREAHTAADGTRFDFV
                     LYEKI"
     gene            complement(570..1784)
                     /locus_tag="VEL04_02575"
     CDS             complement(570..1784)
                     /locus_tag="VEL04_02575"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HYT47284.1"
                     /translation="MGDRPPSSITRTRQNALARQQPQENLFDTHAEVSLDFEIVRRAA
                     RHKYTQPFLILDTAIVRSKVRRFRAAMPRVRPHYAVKANPERRVLKVLAQEGTGFEIA
                     STAELDLLLSLGVNATDVFYSNPVKSRESIAYAAGRGVEWFVVDSVDELRRVFEVKSD
                     AKQYLRIAAPNIGSDWPLSGKFGAGAADAREIVAAAAKLGADLAGITFHVGSQCRNPE
                     NWRVALEKARNLFDAMAKTGLKPRLLNIGGGFPVRHVKPIPSIEVIGQVVNEGLKAFP
                     EEVQVIAEPGRYLVSDAGYFVCRVLGTATRAGKRWMHWDAGLFGGVIEASEGLKYRIR
                     TDRSGPDVSWTVGGPTCDSVDIVMRDEPLPSDLQEGDFVYLSNAGAYTTAYASQFNGF
                     PLPEVRVFESRS"
     gene            complement(2031..2446)
                     /gene="dcd"
                     /locus_tag="VEL04_02580"
                     /pseudo
     CDS             complement(2031..2446)
                     /gene="dcd"
                     /locus_tag="VEL04_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013522100.1"
                     /note="Catalyzes the formation of dUTP from dCTP in
                     thymidylate biosynthesis; frameshifted; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
     gene            complement(2447..3532)
                     /gene="apbC"
                     /locus_tag="VEL04_02585"
     CDS             complement(2447..3532)
                     /gene="apbC"
                     /locus_tag="VEL04_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HYT47285.1"
                     /translation="MSLTEAQIQETLRTLVDPNTGKDFVATRSARNIKVSGDEVALEI
                     ELGYPGKSQQEPIRRQVIQGLKASGAGKVSVSVSSKVVAHAVQRGVKLIPGIKNIIAV
                     ASGKGGVGKSTTAVNLALGLAAEGASVGMLDADIYGPSQPMMLGIAGRPESKDGKHLE
                     PMEGHGLQAISIGFLIDVDTPMVWRGPMVTQALEQLLKDTRWRELDYLVVDLPPGTGD
                     IQLTLAQKVPVTGAVIVTTPQDIALIDARKGLKMFEKVGIPILGVVENMSFHVCPKCG
                     HESHIFGSGGAERMSREYGTELLGQLPLDEAIRRQADSGKPTVVSDPDGKAAEIYRRI
                     ARRCAVKIAESQRDMTSKFPNIVVQNT"
     gene            3589..5805
                     /gene="metG"
                     /locus_tag="VEL04_02590"
     CDS             3589..5805
                     /gene="metG"
                     /locus_tag="VEL04_02590"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF001100.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HYT47286.1"
                     /translation="MPRRMFVTTALPYANGPFHIGHVMEYIQADIWVRFQRMQGHAVH
                     FMGADDAHGAPIMLAAEKAGKSPEAFVAEIARGRKQYLDGFHIAFDNWHSTHSPENTQ
                     LSQDVFRKLKAAGLVYAKPVEQFYDPVKGMFLADRYIKGECPNCGARDQYGDACENCS
                     TVYSATALKNPYSALSGAKPVLESSEHYFFRLSDRKCRAFLKTWLEKPGRLQPQVVNK
                     AKEWLEGTGDKALSDWDISRDPPYFGIRVPDIKEEKYLYVWLDAPIGYFASLKNYCDR
                     ENLDFDAFVKPGGNTQMIHFIGKDIIYFHTLFWPAMLEYSGYKTPDYVFVHGFIGVSG
                     EKMSKSRGTGIDPLRYLELGMNPEWLRYYIAAKLNANVEDLDFNPDDFLARVNSDLVG
                     KYVNIASRAAPFLRNKFGNKLGNLHYSKDVLAWSRTTSAIGIFDFSPGDETVDRLEQE
                     DMIKVVSEGDFVVDPARLLSVGMVASKKIAALYENREFGRATREVMFLADLTNRYVDA
                     TRPWEIAKDPNRLKELQDYCIVAINLFRLLTIYLKPILPSLSEKAEQFLNVRPLIWKD
                     AYSLLEGHSVGNYHHLLTRVEGKQLDQLFDIESVPQAAAPQRHAERQQHAAKEHPVNE
                     PITIDDFNKLDLRVAKIVRAESIEGADRLLKLTLDLGNGTRTVLAGIKSSYAPDKLEG
                     RLTVLVANLAPRTMKFGVSEGMVLAASWDKNEKAGEGPGIFLISPDVGAQPGMRVK"
     gene            5846..6979
                     /locus_tag="VEL04_02595"
     CDS             5846..6979
                     /locus_tag="VEL04_02595"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYT47287.1"
                     /translation="MEGSGRTLVCSVLFLDIAEYTKKPVAEQLQLKQAFNQALGATLE
                     QVAQRDRIILDTGDGAAVTFLGDPEDALFASIAMRDNVASLSVRLGVNLGPVRLVKDL
                     NGQVNIIGDGINVAQRVMSFAQPGQLLVSRSFFEVVSCLSRDYVTLFHHEGSRTDKHV
                     RDHDVYSVVGGTPATRRFADTDPQIRTGGRALSWLRSLGPFGLRHSSLVAAPLAFALV
                     VGGGLVARAMTELPASTSAVGAPAKLAALPAAPAKPAAAPAKPANTPEAPAKPGDASA
                     PKAAAAADKPQPVPKNLIASLEKKLEKGAAAAPSTRLELAVAPWGEVLVDGKSRGVSP
                     PLRVLKVSPGPHTIEIRNSTFPPYIEKVQAKEGEAVKIRHRFR"
     gene            6980..>7570
                     /locus_tag="VEL04_02600"
     CDS             6980..>7570
                     /locus_tag="VEL04_02600"
                     /inference="COORDINATES: protein motif:HMM:NF038027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TssQ family T6SS-associated lipoprotein"
                     /protein_id="HYT47288.1"
                     /translation="MLCRLTAAVLLIALAGCASLFQTSKGAAALSSGLRQYEEGEYVQ
                     SAQNLQGALDEGLSDAERARAHKHLAFIHCASGRERACRSEFRKALAFAPSLELSPSE
                     MGHPAWGPIFRSVKAEATPLNIGLQQFEDGDYVESAKNLQGAIDQGLSDAERARAHKH
                     LAFIHCASAREGACRDEFRKALVLEPSLELAPSEAGH"
BASE COUNT         1399 a         2409 c         2378 g         1384 t
ORIGIN      
        1 gagcaggccg aagaacgacc gcgggtacgc catgtcccta taatttcacg caaattttcg
       61 tgcgagggag gaccaatgtc acacaatggg cagccgcgcg tctacctcgt cgcggccgtc
      121 gctgccaatg gaaccatcgg cgccgcaggc cagctacctt ggcggctgcc cgaggatttg
      181 acgcatttta agcgcgtcac gatggggcac ccggtgatca tggggcggcg cacctgggag
      241 tctctcggca ggccgcttcc cggacgcgag aacatcgtgg tgtcgacccg gcgtggctac
      301 gaggctcccg gcgcggcggt ggcgagctcg ctcgacgccg cgctcgccct gtgcgccggc
      361 gagccggtcg ctttcgtgat cgggggtacg cgtctgttcg aggcagcgct accgctcgcg
      421 agcggactcg taatgaccga gatcaagcgc gactatgccg gcgatgcctg gttcccgcag
      481 tacgaccgtt cccaatggcg cgagtcgcag cgcgaagcgc ataccgccgc ggacggcacg
      541 cgcttcgact tcgtcctgta cgagaagatc tagctcctgg attcgaagac cctcacctcg
      601 ggcaacggga agccgttgaa ctggctcgca taggccgtgg tgtaagcgcc ggcgttgctg
      661 aggtagacga aatcgccttc ctgcaggtcc gagggcagcg gttcgtcgcg catcacgata
      721 tcgaccgaat cgcaggtcgg cccgccgacc gtccaggaga cgtctggtcc cgagcggtcg
      781 gtgcggatac ggtatttcag gccttcgctc gcttcgatca cgccgccgaa caggcccgcg
      841 tcccagtgca tccagcgctt gccggcgcgc gtagcggtgc ccagcacccg gcagacaaaa
      901 tagccggcgt ccgacaccag gtaacgcccc ggttcggcga tcacctggac ctcctcgggg
      961 aaggccttca ggccttcgtt gacgacctgg ccgatgactt cgatggaagg gatgggcttg
     1021 acatggcgca ccgggaagcc gccgccgatg ttgagaaggc gcggctttag accggttttc
     1081 gccatcgcat cgaaaagatt gcgcgccttt tccagggcca cgcgccagtt ttccgggtta
     1141 cggcactgcg agccgacgtg gaacgtgatg cccgcgaggt ccgcgcccag cttcgccgca
     1201 gcggcgacga tctcgcgggc gtcggcagct ccggcgccga acttgccgga caacggccag
     1261 tcgctgccga tgttcggcgc ggcgatccta aggtactgtt ttgcgtcgga ctttacctcg
     1321 aaaacgcggc gcagctcgtc aacgctgtcg actacgaacc actcgacgcc cctgccggcg
     1381 gcgtaggcaa tcgattcgcg cgacttgacc gggttggagt agaacacgtc ggttgcattc
     1441 accccgaggg aaagcaacag gtccagctcg gcggtggagg cgatctcgaa gcccgtgccc
     1501 tcctgcgcca ggaccttgag cacgcgccgc tccgggttcg ccttgaccgc gtagtgcggc
     1561 cgcacgcgcg gcatggccgc gcggaagcgc ctgactttcg agcgcacgat cgccgtgtcg
     1621 aggatcagga aaggctgggt gtacttgtgg cgtgcggccc ggcggacaat ctcgaagtcg
     1681 aggctgactt cggcatgggt gtcgaagagg ttttcttgcg gctgctgcct ggctaaagcg
     1741 ttttgtcttg tgcgtgtgat gctactagga gggcgatcgc ccatatcgaa tttctcccgg
     1801 aatggaccta ggtctcagga atgtcccgaa cgcagatgtc gtacaccggg cacgagttcc
     1861 ttttgcttgt catgatgacc tccttcccct cggtgaaaac aaaaaaactt gcgccgatta
     1921 tagggatgac ccccgcggag tcaagagcct gaacaaagat ttttacggtc cctatagcgt
     1981 cccaggggga cgcgaaccac aatatctagt aggaaggcaa aacccgcgga ctagatacgc
     2041 ggcagggtca cccctttttg cccctggtac ttgcggtcgc ggtacgaggt ctcgcacacc
     2101 tcgtcggact cgaggaagat cacctgcgcc acgccctcgt tggcgtagac ccgcgccggc
     2161 agcggcgtgg tgttggagaa ctccagcgtg acgtgcccct cccattcggg ctcgagcggc
     2221 gtgacgttga cgatcacccc acagcgcgcg taggtcgact cgtcgaattg atgttggtga
     2281 agatcttgaa ctcggtcgag cagcggacat cgtagccgta gctcgaggtg ccgtaggaga
     2341 ccacccggcg tcccgccact tccttcacct gcccgggctc gaacggctcg atcagcttgt
     2401 gctggagcgc catgcggcgg atccagcgat cggacttgat cgtcatctaa gtgttttgca
     2461 cgacgatgtt agggaatttg gaggtcatgt cgcgctgcga ctccgcgatc ttgacggcgc
     2521 agcgccgggc gatgcggcga tagatctccg ccgccttgcc gtcgggatcc gacaccaccg
     2581 tcggcttgcc cgagtcggct tgccggcgga tcgcctcgtc gagcggcaac tggccgagaa
     2641 gctcggtgcc gtattcccgg ctcatgcgct cggcgccgcc cgagccgaag atatggctct
     2701 cgtggccgca tttcgggcag acatggaacg acatgttctc gaccacgccg aggatcggga
     2761 tgccgacctt ctcgaacatc ttcaggccct tgcgcgcatc gatgagcgcg atgtcctgcg
     2821 gcgtggtgac gatcacggcg ccagtgaccg gtaccttctg cgcaagcgtc agctgtatgt
     2881 cgccggtccc cggcggcagg tctaccacca ggtagtcgag ctcgcgccag cgcgtgtcct
     2941 tcaggagctg ctcgagcgcc tgggtgacca tcggcccgcg ccacaccatg ggcgtgtcga
     3001 cgtcgatcag gaagccgatc gagatcgcct gcagaccgtg gccctccatc ggctcgaggt
     3061 gcttgccgtc cttcgattcg gggcggccgg caatgccgag catcatgggc tgcgacggac
     3121 cgtagatgtc ggcgtcgagc attcccaccg aggcgccctc tgcggcgagc ccgagcgcga
     3181 ggttgaccgc ggtggtcgac ttgcccacgc cgcccttgcc cgaggcgacg gcgatgatgt
     3241 tcttgatccc cgggatgagc ttcacgccgc gctgcacggc atgcgccacc actttggagc
     3301 tcacgctgac cgacaccttg cctgcgccgc tagccttgag cccctggatg acctggcggc
     3361 ggatcggctc ctgctggctc ttgcccggat agccgagctc gatctcgagc gcgacctcgt
     3421 cgcccgagac cttgatgttg cgcgccgatc gcgtggcgac gaaatccttg ccggtgttcg
     3481 gatcgaccag cgtgcgcagc gtttcctgaa tttgcgcttc agtgagggac atgcttgaag
     3541 tgtaagctaa aattcgcgcc ttccaagcag atttccagag ccgaatccat gccgcggcgc
     3601 atgttcgtca ccaccgccct gccgtacgcc aacgggccgt tccacatcgg tcacgtcatg
     3661 gaatacatcc aggccgacat ctgggtgcgg ttccagcgga tgcaaggcca tgcggtgcat
     3721 ttcatggggg ccgacgatgc ccacggcgcg ccgatcatgc tggctgcgga aaaagccggc
     3781 aagtcgcccg aagcattcgt cgcggaaatc gcgcgcggcc gcaagcagta cctcgacggc
     3841 tttcacatcg cgttcgacaa ctggcattcc acgcattcgc ccgagaacac gcagctctcg
     3901 caggacgtgt ttcgcaagct caaggcggcc ggcttggtct acgcgaagcc ggtcgagcag
     3961 ttctacgacc cggtgaaagg catgttcctc gccgaccgct acatcaaggg cgagtgcccc
     4021 aactgcggcg cgcgcgacca gtacggcgat gcttgcgaga actgcagcac ggtgtattcc
     4081 gcgaccgcgc tgaaaaatcc ctattcggct ctctcgggcg ccaagccggt gctcgagtct
     4141 tcggagcact attttttcag gctgtccgat cggaagtgca gggccttcct aaagacctgg
     4201 ctcgagaaac cagggcgcct gcagccgcag gtggtgaaca aggccaagga gtggctcgag
     4261 ggaaccggcg acaaggcgct ctcggactgg gatatctcgc gcgacccgcc ctacttcggc
     4321 atccgcgtcc cggacatcaa ggaagaaaag tacctgtatg tgtggctcga cgcgccgatc
     4381 ggctacttcg ccagcctgaa gaactattgc gaccgcgaga atctcgactt cgacgccttc
     4441 gtgaagcccg gcggcaacac gcagatgatt catttcatcg gcaaggacat catctatttc
     4501 cacaccctgt tctggccggc gatgctcgaa tactcgggct acaagacgcc cgactacgtg
     4561 ttcgtacacg gcttcatcgg cgtgtcgggc gaaaagatgt ccaagtcacg cggcactggc
     4621 atcgaccccc tgcgctacct cgagctcggc atgaacccgg agtggctgcg ctactacatc
     4681 gcggccaagc tcaacgccaa cgtcgaggac ctcgacttca atcccgacga cttcctcgcg
     4741 cgcgtcaaca gcgatctcgt cggcaagtac gtcaacatcg cgagcagggc tgcgccattc
     4801 ctgcggaaca aatttggaaa caaactaggc aatttgcact actccaagga cgttcttgca
     4861 tggtctcgca ctacgagcgc gattggtatt tttgacttta gtccgggcga cgagacagtc
     4921 gatcgtctcg aacaagagga catgatcaaa gttgtttctg agggagactt tgttgttgac
     4981 cctgctcgac tgctctctgt cggtatggtt gcttccaaaa agattgcggc cttatacgag
     5041 aatcgtgaat tcgggcgcgc aactcgcgaa gttatgttcc tcgcggactt gaccaataga
     5101 tatgttgacg cgacgcggcc atgggaaatt gcaaaagacc cgaacagact taaagaactg
     5161 caagactact gcattgtagc gataaatctc tttcgattat tgacgatcta tttaaagcca
     5221 atactgccct cattgagcga aaaagccgaa caattcctta acgttcgtcc acttatatgg
     5281 aaggatgcat attcgcttct cgaaggccac agcgttggca actaccatca cctgttgacc
     5341 agggtcgagg gaaagcaact cgatcaactc ttcgatattg aatccgtacc ccaggccgcc
     5401 gcgccgcagc ggcacgccga gcggcagcag catgccgcca aggagcaccc cgtgaatgag
     5461 ccgattacga ttgacgactt caacaagctc gacctgcgcg tggctaagat tgtgcgcgca
     5521 gagtcaatcg agggcgcaga ccggctgctg aagctgactc tggatcttgg caacggcacg
     5581 cgaacggttc tcgcgggtat caaatccagc tacgctccgg acaagctcga gggcaggctg
     5641 accgtcctgg tggcgaatct cgcgccgcgc acgatgaaat tcggcgtctc cgagggcatg
     5701 gtgctcgccg cttcttggga caagaacgaa aaagcaggcg agggaccggg tatatttctt
     5761 atatcccccg atgtgggggc gcagcccggc atgcgcgtaa aataggaccg tagcggccag
     5821 acgcgccgca gagggggtag taccgatgga ggggtccggc cgcacgctgg tctgcagtgt
     5881 gctgtttctc gacatcgccg agtatacgaa gaagccggtt gccgagcagc tgcagctcaa
     5941 gcaggcgttc aaccaggcgc tcggagccac gctcgagcag gtggcgcagc gcgaccgcat
     6001 catcctcgac accggcgacg gtgccgcggt caccttcctt ggcgatccgg aggacgcgct
     6061 gttcgcttcg attgcaatgc gcgacaacgt ggcttccctc tcggtgcgcc tcggcgtcaa
     6121 tctcggaccg gtgcgcctgg tgaaggacct caacggccag gtgaacatca tcggcgacgg
     6181 catcaacgtg gcgcagcggg tgatgagctt cgcgcaaccc ggccagctgc tggtttcgcg
     6241 ttcgttcttc gaggtggtgt cgtgcctgtc gcgcgactac gtgacactgt tccatcacga
     6301 aggttcgcgc accgacaagc atgttcgcga ccacgacgtg tactcggtgg tcggcggcac
     6361 gccagcgacg cggcgtttcg ccgatacgga tccgcagatc cgcaccggcg gccgggcctt
     6421 gagctggctg cggagtctcg ggccgttcgg tctgcgccac tcgtcgctag ttgctgcgcc
     6481 gctcgccttt gcccttgtcg tcggcggcgg cctcgttgcc cgcgccatga cggaactgcc
     6541 ggccagcacg agcgcggtcg gggcgcccgc gaagcttgcc gcgttacccg cggcgccagc
     6601 gaagcccgcc gcagcgccgg cgaagcctgc caacacgcct gaagccccag cgaagcccgg
     6661 cgacgcgtcc gcgccgaagg ccgctgccgc cgcggacaag ccgcagccag tacccaaaaa
     6721 cctcatcgcc agcctcgaga agaagctcga gaaaggcgcc gcagcagcgc cgtcgacccg
     6781 gctcgagctg gcggtggcgc cgtggggcga ggtgctggtc gacggcaaga gccggggcgt
     6841 gagcccgccc ctgcgcgtgc ttaaggtctc gcccggcccg catacgatag agatccgcaa
     6901 ttcgactttt cccccgtaca tcgagaaagt gcaggcgaag gagggggaag cggtgaagat
     6961 tcgccacagg ttcaggtaaa tgctctgcag actgacggcc gcggtgctgc tcatcgccct
     7021 cgcgggctgt gcctcgctgt ttcagacgag caagggtgcg gccgcgctca gcagcggctt
     7081 gcggcaatac gaggaggggg agtacgtcca atcggcgcag aacctgcagg gcgcgctcga
     7141 cgaggggctc agcgacgccg agcgcgcgcg cgcccacaag cacctcgcct tcatccattg
     7201 cgcctcgggc cgcgagcgcg cctgccgcag cgagtttcgc aaagcgcttg ccttcgcgcc
     7261 ctcgctcgag ctctcgcctt cggagatggg tcacccggcg tgggggccga tttttcgctc
     7321 cgtgaaagcc gaggccacgc cgttaaacat cggcttgcag caattcgaag acggcgatta
     7381 cgtcgagtcg gcgaagaacc tgcagggcgc gatcgaccag gggctcagcg acgccgagcg
     7441 cgcgcgcgcc cacaagcacc tcgccttcat ccactgtgcc tcggcccgtg agggcgcctg
     7501 ccgcgacgag tttcgcaaag cgctcgtcct cgagccctcg ctcgagctcg cgccttcgga
     7561 ggcgggtcac
//
LOCUS       DASPXW010000109         2770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_34719, whole genome shotgun sequence.
ACCESSION   DASPXW010000109 DASPXW010000000
VERSION     DASPXW010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34719"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /gene="msrA"
                     /locus_tag="VEL04_02605"
     CDS             complement(<1..339)
                     /gene="msrA"
                     /locus_tag="VEL04_02605"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008342768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HYT47289.1"
                     /translation="MTSQTQAGKEVATLAGGCFWCLEAVYDDLKGVESVESGYMGGKT
                     ANPGYEDVCSGETGHAEVVQLTFDPKSVSFKEILEVFFVIHDPTTPDRQGNDVGTQYR
                     SAIYTYSPEQL"
     gene            443..1051
                     /gene="msrA"
                     /locus_tag="VEL04_02610"
     CDS             443..1051
                     /gene="msrA"
                     /locus_tag="VEL04_02610"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HYT47290.1"
                     /translation="MSLLRNGLAILLAASAFTCAAQQPGGGQAVATFAGGCFWCMEPP
                     YDALPGVISTTSGYMGGTKKNPTYEEVSSGSTGHAEAVQVAYDPKKVGYEKLLEVFWK
                     NIDPTVRDQQFCDVGNQYRTAIFYHTEEQKRLAEASKAALMKTKPFKQEIVTPVVPAG
                     EFWPAEAYHQDFYIKKPVRYKFYRTGCGRDARLKQLWGSAPH"
     gene            1068..2705
                     /locus_tag="VEL04_02615"
     CDS             1068..2705
                     /locus_tag="VEL04_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYT47291.1"
                     /translation="MNAVRPHTASEGANQPPPLAGYNAWSQDRILKAAVGREGGDWIA
                     ERAERMGELVGSERMQALASQANRYTPELKTHDRFGNRIDAVEYHPAYHELMALAFGS
                     GLHSLAWTEKRHGARVARAALNYLWNQGENGVACPVTMSFAAVRVLRNDPGLAAEWEP
                     KLLACDYDPRPLPLALKRAVTIGMAMTERQGGSDLRANSTQAAPLGGGGYELSGHKWF
                     CSAPMSDAFFTLARTGAGLTCFFVPRSLPDGTRNPFFIQRLKDKCGNRSNASSEIEYG
                     GTRARIIGEEGRGIATLIEMAHLTRSDIVVACAGMMRAALNQALHHAAYRSAFGKRLA
                     EHPLMQNVLADLSLESEAATLLAFRLAGAFDRSDDDERERLLMRLMTPIAKYWLCKRV
                     PALAVEAMECLGGNGYVEEAPLARLYREAPLNGIWEGSGNVICLDVLRSIQREPGAVQ
                     TLLEEIGRGGERLRTHAASIALTLKDLGSAESRARRIVETIALALQASLMVQHAEKEP
                     ADAFCASRLEGDWGRAFGTLPAGTRCEAIVRRAQLAF"
BASE COUNT          479 a          939 c          897 g          455 t
ORIGIN      
        1 aagctgctcg ggcgaatagg tgtagatcgc cgagcggtac tgcgtcccga catcgtttcc
       61 ctgccggtcg ggcgtcgtcg ggtcgtggat gacgaagaac acctctagga tttccttgaa
      121 ggacacggac ttcgggtcga aagtgagctg gaccacctcg gcgtggccgg tctcccccga
      181 gcacacgtcc tcgtaaccgg ggttcgcggt ctttcctccc atatagccgg attcgaccga
      241 ctccacgccc ttcaggtcgt cgtatacggc ctccaggcac cagaagcagc cgcccgccag
      301 ggtggcgact tccttccccg cttgcgtctg gctcgtcatg gcgtctgcct gtcctttccg
      361 atttgtcggg acccgacggt gtccattaca gtatgacgat ttccgccgat gtccaagccc
      421 ggcgttctcc ggaggaattg acatgagcct cttgcgaaat ggtctcgcga tcctgttagc
      481 agccagcgcg ttcacctgcg ccgcccagca gcccggcggc ggacaagccg tcgccacctt
      541 cgccggcggc tgcttctggt gcatggaacc accttatgat gccttgccgg gggtgatttc
      601 gacgacctcc ggctacatgg gagggaccaa gaagaacccc acatacgaag aagtctcctc
      661 cggctcgacg ggtcacgcgg aagcggtgca ggtcgcgtac gacccgaaga aggtcggcta
      721 tgagaaactt ctggaagtgt tctggaaaaa catcgatccc acggtgcgcg accagcagtt
      781 ctgcgacgtc ggcaaccaat accgcaccgc gattttctat cataccgagg agcagaagcg
      841 cctcgccgag gcgtccaagg cggcgctcat gaagacgaaa cccttcaagc aggagatcgt
      901 cacgccggtc gtgcccgccg gcgaattctg gcccgcggag gcctaccatc aggacttcta
      961 catcaagaag ccggtgcgct acaagttcta ccgcaccggc tgcggccgcg acgcgaggct
     1021 gaagcagttg tggggaagcg ctccgcacta gagcgccggt tgaattcgtg aacgccgtcc
     1081 gcccgcatac cgcctccgag ggcgccaatc agccgccgcc gctcgcgggc tacaatgcct
     1141 ggtcgcagga tcggatcctg aaggcggcgg tcggacgcga gggcggggac tggatcgccg
     1201 agcgcgccga gcgcatggga gagctggtcg gcagcgaacg catgcaggcg ctcgcctcgc
     1261 aggcgaaccg ctacaccccc gagctgaaga cgcacgaccg cttcggcaac cgcatcgacg
     1321 cggtcgaata ccaccccgcg taccacgaac tgatggcgct cgctttcggc tcgggactgc
     1381 attcgctcgc ctggacggag aagaggcatg gcgcccgggt cgcgcgcgcc gcgctcaatt
     1441 atctctggaa ccagggcgag aacggcgtgg cctgcccggt gacgatgtcc ttcgccgcgg
     1501 tgcgggtgct gaggaacgac cccgggctcg ccgccgaatg ggagcccaag ctcctcgcct
     1561 gcgactacga tccgcggccg ctgccgctcg cgctaaagcg cgcggtgacg atcggcatgg
     1621 cgatgaccga gcggcagggc ggctcggacc tgcgcgcgaa ctcgacccag gccgcgccgc
     1681 tcggcggcgg cgggtacgag ttgtccggac acaagtggtt ctgctccgcg ccgatgagcg
     1741 acgccttctt tacgctcgcg cgcaccgggg ccgggctcac ctgcttcttc gtgcctcgct
     1801 cgcttcccga cggaacccgc aatccttttt tcatccagcg tttgaaggac aaatgcggca
     1861 accgctccaa cgcctcctcc gaaatcgagt acggcggcac gcgcgcgcgc atcatcggcg
     1921 aagaagggcg cgggatcgcc accctgatcg agatggcgca cctgacgcgc tccgacatcg
     1981 tcgtcgcctg cgccgggatg atgcgcgccg cgctcaacca ggcgttgcac catgccgcct
     2041 atcgcagcgc gttcggcaag cggctcgccg agcacccgct catgcagaac gtgctcgccg
     2101 atctttcgct cgagtcggaa gcggccacgc tgctcgcgtt ccgcctggcc ggcgccttcg
     2161 accgctcgga cgacgacgag cgcgagcggc tgttgatgcg cctcatgacc ccgatcgcca
     2221 agtactggct gtgcaagcgc gtgcctgcgc tcgccgtcga ggcgatggaa tgcctcgggg
     2281 gaaacggcta tgtcgaggag gcgccgctcg cgcggctgta ccgcgaggcg ccgctgaacg
     2341 gcatctggga aggctccgga aacgtcattt gcctggacgt gctgcgctcg atccagcgcg
     2401 agccgggagc cgtgcaaaca ctgctggagg agatcgggcg cggcggcgag cgcttacgga
     2461 cgcatgcggc gtcgatcgcg ctgacgctca aggacctcgg cagcgccgaa tcccgggcgc
     2521 gccgcatcgt cgagacgatt gcgctcgcgc tgcaggcgag cctcatggtg cagcacgctg
     2581 aaaaagaacc ggccgacgca ttctgcgctt cgcgcctcga aggcgactgg ggccgcgcat
     2641 tcggcaccct gcccgccggc acccgctgcg aggcgatcgt caggcgggcg cagcttgcat
     2701 tctgaaatac acgacacatc gaccttcgag gcgtcgttcc ctcagaagcg ggattctctc
     2761 ggcccgggcc
//
LOCUS       DASPXW010000110         6368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1042957, whole genome shotgun sequence.
ACCESSION   DASPXW010000110 DASPXW010000000
VERSION     DASPXW010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6368
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042957"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VEL04_02620"
     CDS             complement(<1..312)
                     /locus_tag="VEL04_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47292.1"
                     /translation="MKHFPAVSRLAAAAAFLVAAANACAQPPQKDRKEIRELNEPTLY
                     EFLLGEIALQRGEAALAAQTYLDLAKRTRDPRVARRAVEVASQARQPELAIEAAKIWH
                     EI"
     gene            376..1185
                     /gene="mutM"
                     /locus_tag="VEL04_02625"
     CDS             376..1185
                     /gene="mutM"
                     /locus_tag="VEL04_02625"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HYT47293.1"
                     /translation="MPELPEVEVTRRGLAPRLVGRVISSVAVHQPRLRWPVSQDVRGL
                     AGRTVRSVRRRGKYLLVDCGDGHLILHLGMSGSLRLVPPRTPAGKHDHFDLLLGDRML
                     RLRDPRRFGAVLWTTQRIEAHPLLAHLGVEPLSRALDPARLHALARPHRTSIKQFLMD
                     ARRIVGVGNIYASEALFRARINPRTPSNRVSLEKCKKLAVAIKRTLRAAIRAGGSSLR
                     DFVGADGSPGCFQSRYWVYDREGKACRRCKTKIRRIVQGQRSTYFCPRCQR"
     gene            complement(1182..1664)
                     /gene="coaD"
                     /locus_tag="VEL04_02630"
     CDS             complement(1182..1664)
                     /gene="coaD"
                     /locus_tag="VEL04_02630"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006385942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HYT47294.1"
                     /translation="MIKAVYPGTFDPLTRGHEDLVRRAATLFDSLVLAIADSKAKRTF
                     FSLEERIAMAREVLGGVKHVEVVGFSGLLTDFVRKQGARVVLRGLRAVSDFEYEFQLA
                     GMNRNLYPEMETMFLTPSEQHMFISATLVREIAALGGDVSEFVHPLVAKRLKQKAKSG
                     "
     gene            complement(1633..2223)
                     /gene="rsmD"
                     /locus_tag="VEL04_02635"
     CDS             complement(1633..2223)
                     /gene="rsmD"
                     /locus_tag="VEL04_02635"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HYT47295.1"
                     /translation="MHSNKGRIRIIAGEYRGRRIAVPPRPDLRPTPDRVRETLFNWLG
                     QWLDGHACLDLFAGSGALGFEAASRGAARVVMVENDRGVFTALERTRNLLGARQVELV
                     FGDAFDYLKKAKDRFDVVFLDPPFRQNALPAVMERLPARLSPRARVYAEAAAPLEPRP
                     PWGELKRARAGQVSYQLLQWEGGVKAHDQGGLPGNV"
     gene            2284..3090
                     /gene="ftsY"
                     /locus_tag="VEL04_02640"
                     /pseudo
     CDS             2284..3090
                     /gene="ftsY"
                     /locus_tag="VEL04_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004633434.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
     gene            3083..3733
                     /gene="ftsE"
                     /locus_tag="VEL04_02645"
     CDS             3083..3733
                     /gene="ftsE"
                     /locus_tag="VEL04_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HYT47296.1"
                     /translation="MVSFSAVAKRYPGGKAALHDVSFAVEAGELAFITGRSGAGKSTL
                     LKLIPAIERPSSGSVIVHGHNVGALRRAAIPYLRRNIGLVFQDQKLLYDRSVYDNVML
                     PLAFSDHAPNEAARRARAALDKVGLLAREKANPIELSGGEQQRLAIARAVVNRPALLV
                     ADEPTANLDAESAARVLDIFVAFNQVGVTVLIASHDRGLVERYGKRVLQLSEGKLA"
     gene            3730..4623
                     /gene="ftsX"
                     /locus_tag="VEL04_02650"
     CDS             3730..4623
                     /gene="ftsX"
                     /locus_tag="VEL04_02650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HYT47297.1"
                     /translation="MKAWARQHVAAFRSALRRLSRPAGVLSALVIGVAVSLPAGGYVL
                     LESLREVAARVTLEPQISVFLRPEAKRAEADALGMALRADPRISAVRFIPREEALKQL
                     GAVEGLPDVIASLGRNPLPDAFVVTAAGESLEPLAAELAKLPGVAHVQADTVWARRLT
                     ALARIGRLGLGLLAALLGAGLVAVTFNTIRLQILTLRDEIEVSKLIGATDGFIRRPFY
                     YLGLLQGIAGGAIALGIVAGIIALLNREVRLLAESYGSSFRFALLSGDDAGAVLLLAG
                     LLGWIGAHLSVSRHLRQIEPR"
     gene            4743..5651
                     /gene="rpoH"
                     /locus_tag="VEL04_02655"
     CDS             4743..5651
                     /gene="rpoH"
                     /locus_tag="VEL04_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HYT47298.1"
                     /translation="MDMQATLPLPVSVPLPLSTGSLEAYIQSVSRFPILSAEEERALA
                     RRFREHDDVEAARQLVLSHLRLVVAVARGYLGYGLPHADLIQEGNIGLMKAVKRFDPE
                     RGVRLVSFAIHWIKAEIHEYILRNWRLVKIATTKAQRKLFFNLRSMKSGLAPLTSADV
                     ERVATELKVKPEEVVEMETRLSGHDIGFEADRDDEDAYAPVDYLAADSDSEPYRVLEA
                     EESKRVHAAGLERALASLDPRSRRIIQARWLKEKNPATLHDLAAEFKVSAERIRQIEL
                     KALQKMRKSIGSLDAPRAALPAPAAA"
     gene            complement(5656..>6368)
                     /locus_tag="VEL04_02660"
     CDS             complement(5656..>6368)
                     /locus_tag="VEL04_02660"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HYT47299.1"
                     /translation="LHYQPKVDLETRAIVGVEALIRWQSPELGLVPPMKFIPLLEETG
                     LILQVGSWALKRAALDHRAWVEAQLKAPRVAVNVSPIQLRQRDFVRIVEQAIMEGVAP
                     AGIDLEITESLIMEDIHANIERLLLVRKLGVAVAIDDFGTGYSSLAYLARLPVETLKI
                     DRSFVITMLEDPNTATLVQTMITLAHSFRLKVVAEGVESEEQAKMLRLLRCDQMQGYL
                     FSKPLPNDALVALLRKSS"
BASE COUNT         1033 a         2196 c         2101 g         1038 t
ORIGIN      
        1 gatctcgtgc cagattttcg cggcctcgat cgcaagctcg ggctggcgcg cctggctcgc
       61 gacttcgacc gcgcggcgcg cgacgcgcgg gtcgcgcgtg cgcttggcga gatcgagata
      121 ggtttgcgcg gcgagcgccg cctcgccgcg ctgcagcgcg atctcgccga gcaggaactc
      181 gtacagcgta ggctcgttga gctccctgat ctccttgcga tccttctgcg gcggctgggc
      241 gcaggcgttt gccgcggcca cgaggaacgc ggctgcggcg gcaagcctgg aaactgcggg
      301 gaaatgtttc atcttcggcc ttcgctgcaa gccatcttag gtatatttgg tgccccgcac
      361 aagcgacgct tttcgatgcc ggaactgcct gaagtcgaag taacccgtcg aggtctcgcg
      421 ccgcgccttg tcggccgcgt catctcgtcg gtcgccgtgc accagccgcg cctgcgctgg
      481 ccggtatcgc aggacgtgcg cggccttgcg ggccgcaccg tgagatcggt gcgccggcgc
      541 ggcaagtacc tgctggtcga ttgcggcgac ggccacctca tcctgcatct gggaatgtcc
      601 ggaagcctgc gcctggtgcc gccgcgcacg cccgcgggca agcatgacca cttcgatctt
      661 ctgctcggcg accgcatgct gcggctgcgc gacccgcggc gcttcggcgc cgtgctgtgg
      721 accacgcagc gcatcgaggc gcacccgctg ctcgctcacc tcggggtcga gccgctctcg
      781 cgcgcgctcg acccggcgcg gctgcatgcg ctggcgcggc cgcaccgcac ctcgatcaag
      841 cagttcctga tggacgcgcg gcgcatcgtc ggcgtgggca acatctacgc cagcgaagcc
      901 ctgttccgcg ccaggatcaa tccgcgcacg ccgagcaacc gagtgtcgct tgaaaaatgc
      961 aaaaaacttg ccgtcgcgat caagcgcacc ctgcgcgcag cgatccgcgc cggcggctcg
     1021 agcctgcgcg acttcgtcgg cgccgacggc tcgcccggct gtttccagag ccgctactgg
     1081 gtctacgacc gcgaaggcaa agcgtgtcgt cgatgcaaga cgaaaatccg ccgtatcgtg
     1141 cagggccagc gctcgaccta cttctgcccg cgctgccagc gctaaccgct cttcgctttc
     1201 tgtttcaagc gcttcgccac cagcgggtgg acgaattcgg agacgtcgcc gccgagagcg
     1261 gcgatctcgc gcaccagggt cgccgagatg aacatgtgct gctccgaggg cgtgagaaac
     1321 atggtttcca tctcgggata tagattgcgg ttcatgccgg cgagctggaa ttcgtattcg
     1381 aagtccgaca ccgcgcgcag gccgcgcagc accacgcggg cgccctgctt gcgcacgaaa
     1441 tcggtgagca ggccgctgaa tcccaccacc tcgacgtgct tcacgccgcc gagcacctcg
     1501 cgcgccatcg cgatgcgctc ctccagcgag aaaaacgtcc gcttcgcctt gctgtcggca
     1561 atggcgagaa ccagcgagtc gaacagcgtc gccgcgcggc gcaccaggtc ctcgtggccg
     1621 cgcgtcaacg gatcaaacgt tcccgggtag accgccttga tcatgcgctt ttaccccgcc
     1681 ctcccactgc agaagctggt agctcacctg gccggcgcgg gcgcgcttga gctcgcccca
     1741 tggcggccgc ggctcgagcg gcgccgccgc ttcagcatag acgcgggcgc gcggcgagag
     1801 ccgcgccggc agccgctcca tcaccgccgg cagggcattc tgcctgaacg gcggatcgag
     1861 gaaaaccaca tcgaagcggt ccttcgcctt cttcagatag tcgaaggcgt cgccgaacac
     1921 gagctctacc tgcctcgcgc caagcaggtt tctcgtcctt tcaagagcag tgaagacgcc
     1981 gcggtcgttt tccaccatca ccacgcgcgc agcaccgcgc gaggcggcct cgaagccgag
     2041 cgcgccgctg ccggcaaaaa ggtcgaggca ggcgtggccg tcgagccact ggccgagcca
     2101 gttgaacagc gtctcgcgca cccggtccgg cgtcgggcgc agatcgggcc gcggcggcac
     2161 ggcgatgcgg cggccgcggt attccccggc aataattcga attcgccctt tgttagaatg
     2221 caccgatcca tggtagcagc gctttcctgg gccgcgcgcc tcaaggctgg cctcgcgcgg
     2281 cagcgcgtgg acgagtcgct ctacgcggag ctggaagcgg cgctgctgca ggccgactgc
     2341 ggggtcgatg cgacgcaaac gctggtcgcg tcgcttcgca gccgcaggct cgaggacggc
     2401 gaagcgctca agcgcgcgct caaggatgac attacagaat tattgattcc gttggagcgt
     2461 ccgctgcagc cctcgggtgc aaaacccttt gtcatcatga tcgcgggcgt caacggctcg
     2521 ggcaagacca cctcgatcgg caagctcgcg agatggctgc agctgcaggg caagagcgtg
     2581 ctgctcgccg cgggcgacac gtggcgggct gcagctaggg aacagctcgt cgcctggggc
     2641 gagcgcaaca gcgtgccggt gatcgcgcag gtctcgggcg atccgggtgc ggtggtgttc
     2701 gacgcgcttt cggcggcgcg cgcgcgcggc atcgacgtcg tcatcgccga caccgcgggg
     2761 cggctgccga cccagctcca cctgatggag gaaatccgca aggtgaagcg cgtcgccgcg
     2821 aaggcgctcg agggcgcgcc ccacgaggtg ctgctggtgc tggacgcgaa ctccgggcag
     2881 aactcgctcg cccaggtcag ggcgttcgac gctgcattgg ggcttaccgg gctgatcctc
     2941 acgaagctcg acggcacggc caaaggcggg gtggtttgcg cgatcgcaaa acagaagccg
     3001 gtgccgatcc tgttcgtcgg cgtcggcgaa gacatcgacg acctgcgccc gttcgcggcg
     3061 cgcgatttcg ccgaggcgct cgttggttag cttctccgcg gtcgccaagc gctacccggg
     3121 cggcaaggcg gcgctgcacg atgtgagctt cgccgtggag gcaggcgagc tcgcgttcat
     3181 caccgggcgc tcgggcgccg gcaagtcgac ccttctcaag ctgatcccgg cgatcgagcg
     3241 gccgagctcg ggctcggtga tcgtccacgg ccacaacgtc ggcgctctca ggcgcgcggc
     3301 cattccctac ctgcggcgca acatcgggct ggtgttccag gaccagaagc tgctctatga
     3361 ccgcagcgtc tacgacaacg tgatgctgcc gctcgccttc tccgaccacg cgcccaatga
     3421 agcggcgcga cgcgcacgcg ccgcgctgga caaagtcggc ctgctcgcgc gcgagaaggc
     3481 gaacccgatc gagctctccg ggggcgagca gcagcgcctg gcgatcgcgc gcgcggtggt
     3541 gaaccggccg gcgctgctgg tcgccgacga gccgaccgcg aacctcgacg ccgaatcggc
     3601 ggcgcgcgtg ctcgacatct tcgtcgcctt caaccaagtc ggcgtgacgg tcctgatcgc
     3661 gagccacgac cgcggactcg tcgaacgcta tggaaagcgc gttttgcaac tctcggaagg
     3721 aaagctggcg tgaaggcctg ggcgcgccag catgtcgcgg cgttccgctc ggcgctccgc
     3781 cgcctgtccc ggcccgcggg cgtgctgagc gcgctcgtga tcggggtcgc cgtgtcgctg
     3841 cccgcgggcg gctacgtgct gctcgagagc ctgcgcgagg tggcagcccg cgtcacgctc
     3901 gagccgcaga tttccgtgtt cctgcgaccc gaggcgaagc gtgccgaggc cgatgcgctc
     3961 ggcatggcgc tgcgcgccga cccgcggatc agcgccgtgc gcttcatacc gcgcgaggaa
     4021 gccttgaagc agctcggcgc ggtcgaagga cttcccgacg tcatcgcatc gctcggccgc
     4081 aatccgctgc ccgacgcatt cgtcgtcacg gcggcgggcg agtcgctcga gccgctggcc
     4141 gcggagctgg cgaagctccc cggcgtggcg catgtccagg ccgacacggt gtgggcgcgg
     4201 cggctcactg cgctcgcgcg catcggccgg ctcggcctcg ggctgcttgc ggcgctcctt
     4261 ggcgcggggc tggtcgcggt gaccttcaac accatccggc tgcagatcct cacgctgcgc
     4321 gacgagatcg aggtctcgaa gctgatcggc gccaccgacg ggttcatccg ccggccgttc
     4381 tattacctcg ggctgctgca gggcattgcg ggcggcgcga tcgcgctcgg catcgtcgcc
     4441 gggataatcg cgctcctcaa ccgcgaggtg cggctgctcg cggagtccta tggctcgagc
     4501 ttccgcttcg cgctcctttc gggcgatgat gccggcgccg tgctgctgct cgcaggtctg
     4561 ctaggctgga tcggcgccca cctctcggtg agccgccacc tgcgccagat cgagccgcgc
     4621 taaggatgta cagtaactga acacttacgt tatatcggcc atccgatatg atgaaattca
     4681 cggaaccgga agacctcttt agcactctaa ttacgcgagt gctaaaatct tcctagggag
     4741 aaatggacat gcaagcgacg ctacctctgc ccgtctcagt gcccctgccg ctatccacgg
     4801 ggagcctcga ggcctacatc cagagcgtca gccgcttccc gatcctcagc gccgaagagg
     4861 aacgcgcgct cgcacgccgc tttcgcgaac atgatgacgt tgaagccgcg cgccagctgg
     4921 tgctctcgca cctgaggctg gtggtcgcgg tggcgcgcgg ctacctcggc tacggcctgc
     4981 cgcacgccga cctgatccag gaaggcaaca tcggcctgat gaaggcggtg aagcgcttcg
     5041 acccggagcg cggcgtgcgc ctggtgtcgt tcgcgatcca ctggatcaag gccgagatcc
     5101 acgaatacat cctgcgcaac tggcggctgg tgaagatcgc caccaccaag gcgcagagga
     5161 aactcttctt caacctgcgc tcgatgaaga gcgggctcgc gccgctcacg agcgcagatg
     5221 tcgagcgcgt cgccaccgag ctcaaggtca agcccgagga agtggtcgag atggaaacgc
     5281 gcctctcggg ccacgacatc ggcttcgagg ccgaccgcga cgacgaagac gcctatgcgc
     5341 cggtggatta cctcgccgcc gacagcgact ccgagcccta tcgcgtgctc gaggccgagg
     5401 aatcgaagcg cgtccacgcc gcgggcctcg agcgagcgct cgccagcctc gacccgcgca
     5461 gccgccgcat catccaggcg cgctggctga aggagaagaa ccccgcgacg ctgcacgacc
     5521 tcgccgccga gttcaaggtc tccgccgagc gtatccgcca gatcgagctc aaggcgctgc
     5581 agaagatgcg caaatcgatc ggcagcctcg acgctccgcg cgccgccctc cccgcgcccg
     5641 ccgcggccta ggtttctagg agctcttccg cagcagcgcg acgagcgcgt cgttcggcag
     5701 cggcttgctg aacaggtagc cctgcatctg gtcgcagcgc aggagccgca gcatcttcgc
     5761 ctgctcttcc gactctacgc cttccgcgac caccttcagc cggaacgaat gcgcgagtgt
     5821 gatcatcgtc tgcacgagcg tagcggtgtt cgggtcctcg agcatcgtga tgacgaacga
     5881 acggtcgatc ttgagcgtct cgaccggaag cctggccaga tacgcgagcg aggagtaccc
     5941 ggttccgaag tcatcgatcg ccacggccac gccgagcttg cgcaccagga gcagcctctc
     6001 gatgttcgcg tgaatgtcct ccatgatgag gctctcggtg atctccagat cgatccctgc
     6061 cggagccacc ccttccatga tcgcctgctc cacgatgcgg acgaaatcgc gctgccgaag
     6121 ttggataggc gaaacattga ccgcgacccg cggcgccttg agctgcgcct cgacccacgc
     6181 gcggtgatcg agcgctgccc gcttcagcgc ccaggaaccg acttgcagaa tcaggccggt
     6241 ttcttccagc agcgggatga atttcatcgg cggcacgagc cccagctccg ggctttgcca
     6301 gcgaatcagc gcctcgacgc cgacgatggc cctcgtctcg agatccacct tgggctggta
     6361 gtgcagga
//
LOCUS       DASPXW010000111         6519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_284402, whole genome shotgun sequence.
ACCESSION   DASPXW010000111 DASPXW010000000
VERSION     DASPXW010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6519
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284402"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /gene="dacB"
                     /locus_tag="VEL04_02665"
     CDS             <1..1180
                     /gene="dacB"
                     /locus_tag="VEL04_02665"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HYT47300.1"
                     /translation="LELLGPAYRWKTEVYLDGDNLILKGYGDPKLNYESFWMLLRSLR
                     GRGMREIRGDIVLDRSYFGPGTDERIDDETFRPYNVTPDALLVNFKSLRFTFLPEAER
                     GAVRVFAEPALPGLEIVNSLKIGEGGCPEGRAFRDLIEAAFQAKPPRAAFTGLYPAAC
                     GERDLNVALYSPEDYVAAMIRQLWSEMGGSWNGTVREGVVPPTARLVYVHESETLGEV
                     VRGINKYSNNVMARQLFLTLGAESVGPPAQTENAARAVRQWLALKNIAAPELVLENGS
                     GLSRAERISAANLAALLQAAWKSPVMPEFVSSLPVVATDGTMKKRLRGERIAGYAHIK
                     SGLLSEARAIAGYVLDRNGKRHTVVMIVNHPRAPEADAAIDALLAWVYASRLQSQLAP
                     "
     gene            complement(1157..2617)
                     /gene="ubiD"
                     /locus_tag="VEL04_02670"
     CDS             complement(1157..2617)
                     /gene="ubiD"
                     /locus_tag="VEL04_02670"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041322210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HYT47301.1"
                     /translation="MSYGDLREFLSQLEAKGELKRIAAQVSPRLEMTEICDRVLRSAG
                     PALLFERPAGHSMPVLANLFGTVGRVARAMGVEHAGALREVGKLLAYLKEPEPPKGLR
                     DLWDKFPVFKQVLNMAPKERSSAPVHEVVWEGNQVDLGRLPVQTCWPGDAGPLITWGL
                     TVTRGPVKRRQNLGIYRQQVIGRNRLIMRWLAHRGGALDFQDHVASSNEPFPVAVALG
                     ADPATMLAAVTPIPDTLGEYQFAGLLRGARTEVVKCLTSDLQVPATAEIVLEGAIHPG
                     DTAEEGPHGDHTGYYNEAERFPVLTLERISMRRAPIYHSTYTGRPPDEPAILGMALNE
                     LFVPLLARQFPEIVDFYLPPEGCSYRLAVVSIKKQYPGHAKRVMFGVWSYLRQFMYTK
                     FIVVCDDDIDVRNWKEVVWAIATRVDPARDTLIADNTPIDYLDFASPVSGLGSKMGID
                     ATGKWPGESARRWGRPIVMDAEVKRRVDSLWRELGL"
     gene            complement(2614..3147)
                     /locus_tag="VEL04_02675"
     CDS             complement(2614..3147)
                     /locus_tag="VEL04_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47302.1"
                     /translation="MALSDPLARFEFASGSLRGTQLTLYRSCLLHRGGSELETLPLAA
                     IGSLRVAFERDNRKFGWGAALVVLALLLLAIAAPLSGLASQAVADLTAAGGQGVARAL
                     VSFFRLLEALASLLPVLALACALGGAALAVRGWQGATTLALGLSGFERVYASRGRDAL
                     LLDFAESVSERLMVLER"
     gene            complement(3138..4049)
                     /gene="ubiA"
                     /locus_tag="VEL04_02680"
     CDS             complement(3138..4049)
                     /gene="ubiA"
                     /locus_tag="VEL04_02680"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HYT47303.1"
                     /translation="MDLTPIAKRLDAYERLIRLDKPIGALLLLWPTLWAIWLACQGRQ
                     PTLELLWIFVLGTLLMRSAGCAINDYADRDFDAHVARTRARPLAAREIRAGEALAVAA
                     ALALAAFALVLFLNRLAILLSLVGAVIAASYPFAKRFFALPQLVLGVAFGFGIPMAYA
                     AIQGRLPLECWLLLAANVCYSFAYDTEYAMVDRDDDAKIGIRTSALTLGRWDVAAVMA
                     SYGSMLAILAFLGIRISVSWPYFAGLAVAGAMMAYHWRLIRGRSREGCFKAFMHNNWV
                     GGVIFAGIVASYPGVWPGIWPGVWPWR"
     gene            complement(4063..6090)
                     /gene="recG"
                     /locus_tag="VEL04_02685"
     CDS             complement(4063..6090)
                     /gene="recG"
                     /locus_tag="VEL04_02685"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HYT47304.1"
                     /translation="MKPAVAGLRPGSLERLGLRRGFDFVLHLPLRYEDETALTAPQAA
                     PPGRPVQIEAKVERAKVEFRPKRQLIVHAGNVMLRFFHFYGSQVKQFQRAAEEGLRVR
                     AFGEVRAGWFGAEMAHPRYRIVREGEPLPDALTPIYPATAGLSQAKLRERVLQELDSA
                     QLDDTLPDALRRRYALAGFAESVKLLHRPPPGTDLALLAGRSHPAWRRMKFDELLAQQ
                     LSMRFAYRERRARQAPALPGNGPLLKSFFKSLPFKLTRSQSRAAGEVLRDLAVAHPMQ
                     RLLQGDVGSGKTIVAAIACLAAVDAGGQAAVMAPTEILSEQHFRKFREWLAPLGEQIA
                     WLHGALSRKEKKAALSAIATGKAQVVIGTHALVQEGVEFKRLALVVVDEQHRFGVQQR
                     LMLRKKSGSVVPHQLMMSATPIPRTLSMTYYADLDVSVIDEMPPGRRPVKTRLFSSAR
                     RGEVLTRIRGACAEGQQAYWVCPVIEESKEGDVQTALDTFDKLKKELKNIRVGLLHGR
                     LSAAEKAAVMDDFTKRKTQLLVCTTVIEVGVDVPNASLMVIESAERFGLAQLHQLRGR
                     IGRGASASDCILLYDEPLSGLARDRLKVIYESNDGFKIAQRDLELRGPGEYLGARQSG
                     DPLLRFADLEGDVELVEAAKQAAEGLIKNDLGPARTHVERWLGSRQELTHA"
     gene            complement(6087..6464)
                     /locus_tag="VEL04_02690"
     CDS             complement(6087..6464)
                     /locus_tag="VEL04_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HYT47305.1"
                     /translation="MKSVIQTRDAPEAIGTYSQAVRAGDTVYLSGQIGLDPSTMQLAE
                     GIDAQTHRAFRNLAAVAAAAGLSLDEAVRVTVYLTDLAHFARVNEIMAQYVREPYPAR
                     AALGVAGLPRGALVEIDAILHAG"
BASE COUNT         1132 a         2224 c         2147 g         1016 t
ORIGIN      
        1 gctcgagctc ctcggtccgg cctaccgctg gaagaccgag gtctatctcg atggagacaa
       61 cctcatcctc aagggctatg gcgatccgaa gctcaattac gagagcttct ggatgctgct
      121 gcgcagcctg cgaggacgcg gtatgcgcga aatccgcggc gacatcgtgc tcgaccgcag
      181 ttacttcggt ccagggaccg acgagcgcat cgacgacgag acgttccgcc cgtacaacgt
      241 cacgcccgac gcactgctag tcaatttcaa gtcgctgcgc ttcactttct tgccggaagc
      301 cgagcgcggc gcggtgcgcg tattcgccga gccggcgctg ccaggcctcg agatcgtcaa
      361 ctccctgaaa atcggcgagg gcggctgccc ggaaggacgc gcgtttcgcg acctgatcga
      421 ggctgcgttc caggcgaaac cgccgcgcgc cgcttttacc ggcctctacc ctgcggcctg
      481 tggcgagcgt gatctcaacg tcgctctgta tagccccgag gactatgtcg cagccatgat
      541 ccgccagctt tggagcgaaa tgggcggcag ctggaacgga acggtgcggg agggcgtcgt
      601 gccgccgacg gcgcggctcg tctacgtgca cgagtccgag acgctcggcg aggtcgtgcg
      661 cggcatcaac aagtactcga acaacgtgat ggcgcggcag ctgttcctca cgcttggcgc
      721 tgaatcggtc ggcccgccgg cgcagaccga gaatgcggcg cgcgcggtgc gccagtggct
      781 cgcgctcaag aacatcgccg ctcccgagct ggtgctggaa aacggctccg ggctctcgcg
      841 cgccgagcgc atcagcgcgg cgaacctggc cgcgctgctg caagccgcat ggaaaagccc
      901 ggtgatgccc gaatttgtct cgtcgctacc ggtggtcgca accgacggca ccatgaaaaa
      961 acgcctgcgc ggggagcgca tcgccggata cgcgcacatc aagtccgggc tgctgtcgga
     1021 ggcgcgcgcg atcgcgggct acgtgctcga ccgcaacggg aagcgccaca cggtggtgat
     1081 gatcgtcaat caccccagag cgccggaggc cgacgcggcg atcgacgcgc tgctcgcatg
     1141 ggtgtacgcc tctcgcctac agtcccagct cgcgccatag ggaatccacg cggcgcttca
     1201 cttcggcgtc catgacgatc ggccggcccc agcgccgcgc gctctcgccc ggccacttgc
     1261 cggtcgcatc gatgcccatc ttcgagccga gaccggaaac cgggctggca aagtcaaggt
     1321 agtcgatcgg cgtgttatcc gcgatcagcg tgtcgcgcgc cgggtcgacg cgcgtcgcaa
     1381 tcgcccacac cacctctttc cagttgcgca cgtcgatgtc gtcgtcgcat accacgatga
     1441 acttggtgta catgaactgc ctgagatagc tccagacgcc gaacatcacc cgcttcgcgt
     1501 gcccgggata ctgcttcttg atgctgacca ccgcgagacg gtacgagcag ccctcgggcg
     1561 gcaggtagaa atcgacgatc tcggggaact gcctggcgag cagcggcacg aacagctcgt
     1621 tcagcgccat gcccaggatc gcgggctcgt cgggcggtcg tccagtatag gtcgagtggt
     1681 agatcggcgc gcggcgcatg ctgatgcgct cgagcgtcag caccggaaaa cgctccgcct
     1741 cgttgtagta cccggtgtgg tcgccgtgcg ggccctcctc ggccgtgtcg cctggatgga
     1801 tcgcgccctc gagcacgatt tcggccgtgg cgggaacttg aagatcgctg gtcaagcatt
     1861 tcacgacttc ggtgcgggcg ccgcgcagca accccgcgaa ctggtattcg ccgagcgtat
     1921 cgggaatcgg cgtcacggcg gccagcatgg tcgccggatc ggcgccgagc gcgacggcta
     1981 cagggaaggg ctcatttgaa gaggcaacat gatcctgaaa atcgagcgcg ccgccgcggt
     2041 gcgcaagcca gcgcatgatc aggcggttgc gtccgattac ctgctgccgg tagatgccga
     2101 ggttctgccg gcgcttcacg ggtccgcgcg tgactgtgag gccccaggtg atgagcggac
     2161 ccgcgtcccc gggccagcag gtctgcaccg gcaggcggcc cagatcgacc tggtttcctt
     2221 cccagacgac ctcgtggacc ggcgccgacg agcgttcctt cggcgccatg ttgagcacct
     2281 gcttgaacac gggaaacttg tcccacagat cgcgcagccc cttgggaggc tcgggctcct
     2341 tcaggtaggc cagcagcttt ccgacctcgc gcagggcgcc ggcatgctcc acccccattg
     2401 cgcgagcgac gcgccccacg gtgccgaaca ggttcgcgag caccggcatc gagtgcccgg
     2461 ccggccgttc aaacagcagc gccgggccgg cgctgcgcaa cacccgatca cagatctcgg
     2521 tcatctcgag ccggggcgaa acctgggcgg cgatgcgctt cagctccccc ttcgcctcga
     2581 gctgggaaag gaactcccgc agatcgccat agctcaccgc tcgagcacca tcagccgctc
     2641 gctcacggat tcggcgaagt cgagcagcag cgcgtcgcgc ccgcgtgacg cgtagacgcg
     2701 ctcgaagccc gacaggccca gcgcgagcgt cgtcgcgccc tgccagccac gcacggcgag
     2761 cgcggcgccg ccgagcgcgc aggcgagcgc aagcaccggc agcaggctcg ccagcgcttc
     2821 gagcaggcga aagaaactca ccagcgcgcg cgccacgccc tggcctccgg cggccgtcaa
     2881 atccgcgacc gcttgcgagg ccaggcccga gagcggggcg gcgatcgcga gcagcagcag
     2941 cgcgagcacc accagcgccg cgccccagcc gaactttcga ttgtcgcgct cgaacgcgac
     3001 gcgcagcgag ccgatcgcgg cgagcggcaa agtctcgagc tcgctgccgc cgcggtgcag
     3061 gaggcagctg cggtacagcg tcagctgcgt gccgcgcagc gagccgctcg cgaattcgaa
     3121 gcgcgccagg ggatcactca gcgccatggc cagactccgg gccagatccc gggccaaacc
     3181 ccggggtagc tcgccacgat gccagcgaag attacgccgc cgacccagtt gttgtgcatg
     3241 aaggccttga agcagccttc gcggctgcgg ccgcggatca gccgccagtg gtaggccatc
     3301 atcgcaccgg cgacagcgag ccccgcgaag tacggccagg aaacggaaat acgaattcca
     3361 agaaacgcaa gtattgccag catcgatccg tagctcgcca ttaccgcggc gacatcccag
     3421 cggcccagcg tgagcgcgga ggtgcggatg ccgattttcg catcgtcgtc gcgatcaacc
     3481 atggcgtact cggtgtcgta ggcgaaggaa tagcagacat tcgctgcgag aagcagccag
     3541 cactcgagcg gcagccgccc ctggatcgcc gcgtaagcca tcggaatgcc gaagccgaag
     3601 gcgacgccga ggaccagctg gggaagcgcg aagaagcgtt tggcgaacgg atagctcgcc
     3661 gcaatcacgg cgccgacgag cgaaagcagg atcgcgagcc ggttgagaaa aagcaccagc
     3721 gcgaaggcgg cgagcgccag cgccgccgcc accgcgagcg cctcgccggc acggatctcc
     3781 cgggcagcga gcgggcgcgc gcgcgtgcgc gcgacgtgcg catcgaagtc gcgatcggcg
     3841 tagtcgttga tggcgcaacc cgccgagcgc atgagcagcg tcccgaggac gaagatccaa
     3901 agcagctcga gcgtcggctg ccggccttgg cacgcgagcc agatcgccca cagcgtcggc
     3961 cacaacagga gcagcgcgcc gatcggcttg tcgagacgga tcagccgctc gtaggcgtcg
     4021 agacgcttcg cgatgggtgt caggtccatg cccaagcata actcacgcgt gcgtgagctc
     4081 ctgcctcgat ccgagccaac gctccacgtg cgttcgggcc ggccccaaat cattcttgat
     4141 gaggccttca gccgcctgct tcgcagcttc gacgagctcg acgtcgccct caaggtcagc
     4201 aaagcgcagc agcggatcgc cgctctgccg cgctcccagg tattctcccg ggccgcgcag
     4261 ctcgagatcg cgctgcgcga tcttgaagcc gtcgttactc tcgtagatca ccttgagccg
     4321 gtcgcgcgcc aggcccgaaa gcggttcgtc gtaaagcagg atgcagtcgc ttgcgctggc
     4381 accgcgcccg attcgtccgc gcagctggtg cagctgcgcg aggccgaaac gctcagcgct
     4441 ctcgatcacc atcagcgagg cgttcggtac atcgactccg acttcgatta ccgtggtgca
     4501 aaccaggagc tgggttttcc gctttgtgaa atcgtccatc accgcggcct tttccgcggc
     4561 cgacaaccga ccgtggagca gcccgacacg gatatttttg agttccttct tgagcttgtc
     4621 gaatgtgtcg agcgcggttt gcacgtcgcc ttccttcgat tcctcgatca ccggacacac
     4681 ccagtacgct tgctggcctt cggcgcaggc accgcggatg cgcgtaagaa cctcgccgcg
     4741 ccgcgctgag gagaacaggc gagttttcac cggccggcgc cccggtggca tctcatcgat
     4801 gaccgagaca tcgaggtcgg cgtagtaggt catcgaaagc gtgcgcggga tcggcgtcgc
     4861 gctcatcatc agttggtgcg ggaccacgct tccgctcttt tttcgcagca tgagacgctg
     4921 ctggacgccg aagcggtgct gctcatcgac cacgacgagc gccaagcgct tgaattccac
     4981 accctcctgc accagcgcgt gcgtgccgat gacgacctgc gccttgcctg tggcgatcgc
     5041 cgagagcgct gcctttttct ctttcctcga cagggcaccg tgcaaccagg cgatctgttc
     5101 gccgagcggc gcgagccatt ctctgaactt gcgaaagtgc tgctcggaga ggatctcggt
     5161 cggggccatc actgcggcct gcccgccggc gtccaccgcg gcgaggcagg cgatcgccgc
     5221 gacgatggtc ttgccgctgc cgacatcgcc ttgcagcagc cgctgcatcg ggtgcgcgac
     5281 ggcgagatcg cgcagcactt cgcccgcggc gcgcgactgg ctgcgggtca gtttgaacgg
     5341 cagggacttg aaaaacgact tgaggagcgg tccgtttccc ggcagcgcgg gcgcctgtct
     5401 cgcccgccgc tcgcggtatg cgaagcgcat cgagagctgc tgcgcgagca gctcgtcgaa
     5461 cttcatgcgg cgccacgccg gatggctgcg gccggcaagc aaggcgaggt cggtccccgg
     5521 cggcgggcga tgcagcagct tcacgctctc ggcgaaaccg gcaagcgcgt agcgccgccg
     5581 cagtgcatcg ggaagcgtat cgtccagctg cgcgctgtcg agctcctgca acacccgctc
     5641 gcggagcttt gcctgcgaca ggcccgcggt cgccggatag atcggcgtca gcgcatcggg
     5701 cagcggctcg ccctcgcgca cgatgcggta gcgcgggtgc gccatctcgg cgccgaacca
     5761 gccagcgcgc acctcgccga atgctcgcac gcgcaagccc tcctcggccg cgcgctggaa
     5821 ctgcttcacc tggctgccgt agaaatggaa gaagcgcagc atcacgttgc cggcgtgcac
     5881 gatgagctgg cgcttcggcc ggaactccac ttttgcgcgc tcgactttgg cctcaatctg
     5941 aaccgggcgt cccggcggtg ccgcttgcgg cgccgtgagc gcagtttcat cctcatagcg
     6001 caacggcaga tgcagcacga agtcgaagcc ccggcgcagc ccgagccgct cgagcgagcc
     6061 cggtctcaaa cccgctacgg caggtttcac cccgcgtgga ggatggcgtc gatctcgacc
     6121 agcgcgccgc gcggcaggcc ggcgacgccg agcgcggcgc gcgccgggta cggctcgcgc
     6181 acgtactgcg ccatgatctc gttgacgcgc gcgaagtgcg cgagatcggt gaggtagacg
     6241 gtgacgcgca cggcctcgtc caggctgagc ccggccgcgg ccgcgaccgc ggccaggttg
     6301 cggaacgcgc gatgcgtctg ggcgtcgatg ccctcggcga gctgcatcgt cgacggatcg
     6361 agaccgatct gccccgagag atacacggtg tcgcctgcgc gcaccgcctg cgaataggtg
     6421 ccaatcgcct cgggcgcgtc gcgcgtctgg atcacgctct tcatggcgag tatcttagcg
     6481 taatgatttc cagcctagag aagctgctcg acacgccgc
//
LOCUS       DASPXW010000112         4773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1297111, whole genome shotgun sequence.
ACCESSION   DASPXW010000112 DASPXW010000000
VERSION     DASPXW010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297111"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..1113)
                     /gene="arsS"
                     /locus_tag="VEL04_02695"
     CDS             complement(148..1113)
                     /gene="arsS"
                     /locus_tag="VEL04_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012732795.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM
                     (seleno)protein ArsS"
                     /protein_id="HYT47306.1"
                     /translation="MHATLPLMRRYGFPALGRARLETLQVNLGYKCNQSCVHCHVNAG
                     PQRTELMSRETITDVLVFLQVSGVKTLDVTGGAPELNPHFRDLVLRARQQGAHVIDRC
                     NLTILEEPGQEGLAEFLAAQHVEVIASLPCYTEELVDRQRGKGVYQKSIRALQTLNRL
                     GYGLPGSGLALNLVYNPQGPSLPPAQDKLEADYKRVLQAEHGIVFNRLYTLANMPIQR
                     FGSMLVSKGQLDDYMALLQGSHSAANLEAVMCRTLISVDWQGYVYDCDFNQMLGLPLV
                     VDGRKRVHLSELIGRDLSGNPIAVRDHCYGCTAGQGSSCAGALHG"
     gene            complement(1154..1699)
                     /locus_tag="VEL04_02700"
     CDS             complement(1154..1699)
                     /locus_tag="VEL04_02700"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HYT47307.1"
                     /translation="MPDRPQQLAHLEFLADATKFAEQIRDLIPKCRLLGNFSPAEVLL
                     LAHFMDVYRAEPGVEIIREGDGGDFMLMLIEGKVEVHKRDRWNTPQLLAVVEAGRTLG
                     EMSMIDGEARFATCIAVEPTLVAVLDRENLARIIVEQPLLGAKILMELVLMLSQRLRT
                     TSQRLLGLLDEQSHGAAPNML"
     gene            complement(1728..2834)
                     /locus_tag="VEL04_02705"
     CDS             complement(1728..2834)
                     /locus_tag="VEL04_02705"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HYT47308.1"
                     /translation="MTTPIDSTLERTPDKTSLTDDERIENIVPLPPPEHLIRFFPIQG
                     TAVETLVTETRRKLRQILHGRSERLLVVIGPCSIHDPVAALEYAKKLQEERKRHSDEL
                     EVLMRVYFEKPRTTVGWKGLINDPYMNGSFRINEGLRIARDLLVRINQAGVPAGCEFL
                     DVISPQYIGDVVSWGAIGARTTESQVHRELASGLSAPVGFKNGTDGNVRIAVDALLAA
                     RQKHHFLSVHKNGQVAIVETRGNQDCHIILRGGKQPNYDAGSVGAACEELARAKLPER
                     LMIDCSHANASKDYRRQAGVAADIAAQIGSGEKRIIGVMVESHLVEGRQDLSRGKPLK
                     FGQSITDACLGWQDSAKLLEILAEAVRKRRKHRK"
     gene            3315..4280
                     /locus_tag="VEL04_02710"
     CDS             3315..4280
                     /locus_tag="VEL04_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007749790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYT47309.1"
                     /translation="MSANVAIPIAGFRDVYDVAGVEKALQELSPGANEALRAVYEKML
                     RLGGQRFTVKPSALPAMQAMFDELPNFTAVLEDIRKHLALCIDSDDFIELPPMLLLGE
                     PGIGKTHFARKIAQLLGTGFGFVPMSSLTAGWVLSGASSQWKNAKPGKVFDTFLNGEY
                     ANPVIVVDELDKASSDGQYDPLGALYELLEVETATRFVDEFVELPIDASGAVWLATAN
                     DASRIPEPLLNRLSVYEIEAPDAEGAARIAMSIYREIRNAHDWGRQFPETPSEAALAK
                     LASLPPREMRRAIHSAFGNAKLAERAEVSADDIQDTRGRKARIGF"
     gene            complement(4277..>4773)
                     /locus_tag="VEL04_02715"
     CDS             complement(4277..>4773)
                     /locus_tag="VEL04_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47310.1"
                     /translation="IDTTALRSRAWVSFYRGRWREAYADAAKYVHDTEAKEPDAAYAV
                     ILGALALRRAGKQQEATKFISEWRPRLDAARWPAPVIEYLDGRMQESKLLTAARGPGE
                     RTEALAYAGASLLAAGQQQKGIDVLQRVLREGEPGYLEYDLAYHELRRLGLAAPSDRR
                     EPKR"
BASE COUNT          786 a         1558 c         1574 g          855 t
ORIGIN      
        1 tacaggtcga gcagacgctc cagggcgaac cgattgccgt acaggccgcc ggcgacccac
       61 agcgtgtcga catgcaactg agcggttgca cgcagcgcgg ccgcgccgta gcggtacgaa
      121 agcgggcagc cgcgcccagg cgccgcgcta tccatgcaag gcgcccgcgc acgaagaccc
      181 ctgtccggcg gtgcagccgt agcaatggtc gcgtacggcg atcggattgc ccgagagatc
      241 gcgcccgatg agctccgaga ggtgcacgcg cttgcgccca tcgacgacca gcggcaggcc
      301 gagcatctgg ttaaagtcgc agtcgtagac gtagccctgc cagtcgaccg agatgagcgt
      361 gcggcacatc accgcttcga ggttcgctgc cgagtgcgag ccctgcagca gcgccatgta
      421 gtcgtcgagc tgccctttcg acaccagcat cgagccgaaa cgctggatcg gcatgttggc
      481 gagcgtatac aggcggttga agacgatgcc gtgctcggct tgcaggacgc gcttgtagtc
      541 agcctcgagc ttgtcctgcg ccggcggcaa cgacggcccc tgcgggttgt aaaccaggtt
      601 gagcgcgagg ccgctgccgg gcaggccgta gcccaggcgg ttcagggtct gcaatgccct
      661 gatgcttttc tgatagacac ccttgccgcg ctgccggtcg accagctcct cggtatagca
      721 aggcagcgaa gcgattacct cgacgtgctg cgcagcgagg aactcggcca ggccttcctg
      781 cccgggctcc tccaggatgg tgaggttgca gcggtcgatg acgtgcgcgc cctgctggcg
      841 cgcgcgcagc acgagatcgc gaaaatgagg attcagctcg ggcgcgccgc cggtgacgtc
      901 gagtgttttt actcccgaga cctggagaaa gaccagcaca tcggtgatcg tctcgcgcga
      961 catcagctcg gtgcgctgcg ggccggcgtt gacgtggcag tgcacgcagc tctggttgca
     1021 cttgtagccg agattgacct gcagcgtttc caggcgcgcg cgcccgagcg ccgggaagcc
     1081 gtatcgccgc atcaggggga gcgtggcgtg catgcgcttg tagtcgctct gccggggcgc
     1141 agccttacgg cgcctagagc atgttcggcg cggcgccgtg cgactgctcg tcgagcagtc
     1201 cgagcagccg ctggctggtg gtgcgcaggc gctgcgacag catcagcacc agctccatga
     1261 ggattttcgc gccgagcagc ggctgctcga cgatgatccg cgccaggttt tcgcggtcga
     1321 gcaccgcgac cagcgtcggc tcgaccgcga tgcaggtcgc aaagcgcgcc tcgccgtcga
     1381 tcatcgacat ctccccgagc gtcctgccgg cctcgaccac cgcgagcagc tgcggcgtgt
     1441 tccagcgatc gcgcttgtgc acctcgacct tgccttcgat cagcatcagc atgaagtcgc
     1501 cgccgtcgcc ctcgcggatg atctcgacgc cgggctcggc gcggtagacg tccatgaagt
     1561 gcgccagcaa cagcacttcg gccggcgaga aattccccag cagccggcat ttcgggatca
     1621 ggtcgcggat ctgctccgcg aacttggtgg cgtcggcgag gaactcgagg tgcgcgagtt
     1681 gttgcgggcg gtctggcatg gggctcgcgt ccgattatgc cgaatcccta cttccggtgc
     1741 ttgcggcgct tgcgtacggc ctcggcgagt atttcgagca gcttcgccga gtcctgccag
     1801 ccgaggcagg cgtcggtaat gctctggccg aacttgagcg gcttgccccg gctcaggtcc
     1861 tggcggcctt cgaccaggtg cgactcgacc atcacgccga taatgcgctt ctcgccggag
     1921 ccaatctgcg cagcgatgtc ggccgcgacg ccagcttggc gcctgtagtc cttggaagca
     1981 ttggcatgcg agcaatcgat catcagccgc tcgggcagct tggcgcgcgc cagctcctcg
     2041 caggccgcgc cgacgctgcc cgcgtcgtag ttgggctgct tgccgccacg caggatgatg
     2101 tggcagtcct ggttaccgcg cgtctcgacg atcgccacct gcccgttttt gtggaccgag
     2161 aggaaatggt gcttttgacg cgcggcaagc agggcgtcca cggcaatgcg gacattgcca
     2221 tcggtgccgt tcttgaaccc cacgggcgca gatagcccgg aggcgagctc gcggtgcacc
     2281 tggctttccg tggtgcgcgc gccgatggcg ccccaggata cgacatcgcc gatgtactgc
     2341 ggcgaaatca cgtcgaggaa ctcgcagccc gccggcaccc ccgcctggtt gatgcgcacc
     2401 agcaggtcgc gcgcgatccg caacccctcg ttgatgcgga agctgccgtt catgtacggg
     2461 tcgttgatca ggcccttcca gccgaccgtg gtgcgcggct tctcgaaata cacccgcatc
     2521 agcacctcga gctcgtcgct gtgacgcttt ctttcttcct gcagcttttt tgcatattcc
     2581 agtgccgcaa cagggtcgtg gatcgaacac ggcccgatca cgaccagcag ccgctcggag
     2641 cggccgtgca ggatctgccg cagcttgcgc cgcgtctcgg tgaccagcgt ctccaccgcc
     2701 gtgccctgga tcgggaaaaa gcggatcagg tgctcgggcg gcggcagcgg gacgatgttc
     2761 tcgatgcgct cgtcgtcggt gagcgaggtc ttgtcgggcg tccgctcgag ggtgctgtcg
     2821 atgggcgtgg tcatgtgtgt gcgccttggt tggcccaaaa aaaaaccgcc aggcttatgg
     2881 cgctggcggt ttctcgggat tcgcgggtgt gtggctagcc gttcccctcc gccagctggc
     2941 gcgggaagta gaagaaaaat ccgaaaaagc agcggctcat gtcaccctcg gaacggattc
     3001 aagcacgaac cgcccgccgc tggcaaatgc gcactatttc ggccgcatct tcgggaagcc
     3061 tcgggcgtca ccgtgattat ttccacgccg acgctctgaa agatgccctt cgccgaaacc
     3121 attgaagtac tcgcgcgttc ttatagtcat gccgggctac tgtaccctgc tgttgcgtct
     3181 ttcctccgaa ttggcactca tcgccgagga ctgccaggca aggagtttgc ttggtggtaa
     3241 gcatcatgct agcgttttca ctcggattgg cagcaccggc gaaaaaaagc caaaaaaagg
     3301 aagtgaggtg acaaatgtcg gctaacgtcg ctattccgat cgcgggtttc agggacgtct
     3361 acgacgtcgc gggcgtggaa aaagccctgc aggagctttc gcccggcgcc aacgaggcgc
     3421 tgcgcgcggt gtacgagaaa atgctgcgcc tcggcggcca gcgcttcacc gtgaagccct
     3481 cggcgcttcc cgcgatgcag gcgatgttcg acgagctgcc caacttcacc gcggtgctcg
     3541 aggacatccg caagcacctc gcgctgtgca tcgactcgga cgatttcatc gagctgccgc
     3601 cgatgctgct gctcggcgag cccggcatcg gcaagaccca tttcgcgcgc aagatcgcgc
     3661 agctcctcgg aaccggcttc ggcttcgtgc cgatgagctc gctcactgcc gggtgggtgc
     3721 tctcgggagc ttcctcgcaa tggaagaacg ccaagcccgg caaggtgttc gacaccttcc
     3781 tcaacggcga gtacgccaat ccggtgatcg tggtggacga gctggacaag gcgagctcgg
     3841 acgggcagta cgacccgctg ggcgcgctct acgagctgct cgaggtggaa acagccaccc
     3901 gattcgtgga tgagttcgtg gaactgccga tcgacgcttc aggcgcggtg tggctcgcga
     3961 ccgcgaacga cgcctcgcgc atccccgagc cgctgctcaa ccggctttcg gtctacgaga
     4021 tcgaggcgcc ggacgcagaa ggcgccgcgc gcatcgccat gtcgatctat cgcgagatcc
     4081 gcaacgccca cgactgggga cggcagttcc ccgagacgcc ctcggaggcc gcgctcgcga
     4141 agctcgcctc gctgccgccg cgcgagatgc ggcgggcgat ccactccgcc ttcggcaacg
     4201 cgaagctcgc cgagcgcgcc gaggtaagcg ccgacgatat ccaggacacg cgcgggcgca
     4261 aggcgcgcat cggcttctag cgcttcggct cgcgccggtc ggagggcgcg gcaaggccga
     4321 ggcggcgcag ctcgtggtaa gcgaggtcgt actccagata gcccggctcg ccctctcgca
     4381 atacgcgctg cagcacgtcg attcctttct gctgctgtcc cgcggctagc aggctcgcgc
     4441 cggcatacgc cagcgcttcg gtgcgctcgc caggtcctcg ggcggcggtg agcagcttgc
     4501 tttcttgcat cctgccgtcc aggtattcga ttaccggcgc gggccatctg gcagcgtcga
     4561 ggcgcggcct ccattccgag atgaatttcg tcgcctcctg ctgcttgccc gcgcgccgca
     4621 gggcgagtgc gccaaggatg accgcgtagg cagcgtcggg ttccttcgcc tcggtgtcgt
     4681 gcacgtattt cgcggcgtct gcataggcct cgcgccagcg tccgcggtag aagctcaccc
     4741 aggcgcggga gcgaagcgcc gtcgtgtcga tgc
//
LOCUS       DASPXW010000113         4193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_539080, whole genome shotgun sequence.
ACCESSION   DASPXW010000113 DASPXW010000000
VERSION     DASPXW010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539080"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VEL04_02720"
     CDS             <1..348
                     /locus_tag="VEL04_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013206616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HYT47311.1"
                     /translation="KRTALGEYPRHGRGGQGVIAIQTSERNGSLVGAVLVDDHDEVML
                     ISTGGVLIRTGVAQIRSQGRSTQGVTLIALGDGEKLAGLERIEEGETNGNGDGSDGES
                     SPPQVSPAPPTLH"
     gene            369..1445
                     /gene="serC"
                     /locus_tag="VEL04_02725"
     CDS             369..1445
                     /gene="serC"
                     /locus_tag="VEL04_02725"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HYT47312.1"
                     /translation="MQRIFNFSAGPAMLPAEVLSRAGDEMLDWRGSGMGVMEMSHRGK
                     QFIGIAADAERDLRELLAVPQSCKVLFLQGGATLQFAQVPMNLLHGRGKADYVSTGEW
                     SKRAIKEAKAYCDVHIAASSEDKNFTYAPKRWNVRKDAAYVHYCSNETIGGVEFHSVP
                     YQGEVPVVADASSHFLSRPIDVAKFGLIYAGAQKNVGPAGLSIVIVREDLIGRAAKGT
                     PSVMDYKLQADADSMLNTPPSYSIYIAGLVFKWLKQLGGVAEIEKRNIQKAKLLYEFL
                     DASAFFSNPVSREDRSRMNVPFTLKDASLDETFLKGAAERGMVQLKGHRSVGGMRASI
                     YNAMPLEGVQRLVAYMQEFEKKRG"
     gene            1438..2625
                     /locus_tag="VEL04_02730"
     CDS             1438..2625
                     /locus_tag="VEL04_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HYT47313.1"
                     /translation="MAKAKKCEILVINQIAQVGLKRFPAERYAVVKESKDPWAILVRS
                     QDLHQYEFGPALRALGRAGAGVNNIPVAALSRRGIPVFNAPGANANAVKELVLAGMLI
                     AARNLAPALDFVREIDSSENLDQRVESGKKQFAGFELPGHTLGVVGLGKIGSLVADAA
                     IRLGMNVLGYDPHITVEAAWSLPSQVRRATSVDEILKSSHFVSLHVPFGDKTRHLVSA
                     KNIEQFRSGAVLLNFSREGVVADDAVLKGLASHKLQWYVTDFPSAALIGRPGVIALPH
                     LGASTGEAEDNSAVMVVDEVRDYLENGNLQNAVNFPDASIARGAPFRLAIANANVPDM
                     LGRISHALGRRKINIHDMLNKSRGEMAYTLVDVDSPVPGEALDELSKLGGVLAVRYLP
                     VDE"
     gene            2618..3688
                     /gene="pheA"
                     /locus_tag="VEL04_02735"
     CDS             2618..3688
                     /gene="pheA"
                     /locus_tag="VEL04_02735"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HYT47314.1"
                     /translation="MSNDIDRLRREIDALDDELVALLNRRAGLAANIGSLKSGALAYR
                     PERESEILRRVTKHQGLLSAERIAAVFREVISACRGLEQAMRVAYLGPEGTFSEQAVR
                     KHFGRAVGALPAASVDEAFRRCESGATQFTVVPVENSTEGVVGRTLDLLLATPLRICA
                     EIELRVQQNLLSKENALKKIKRIYSHSQSLAQCNAWLAQNLPAVEKVPVASNAEAAQR
                     AAKEKGAAAIAGEAAAERYGLAALARAIEDSPNNTTRFLVLGNLDPAPTGNDRTSLVM
                     SAENRPGAVHALLTPLAEHRVSMTRIESRPSRSRGSLWEYVFFIDLEGHQQDEKVAAA
                     LAGLRQKAPFLKILGSYPVAVA"
     gene            3693..>4193
                     /locus_tag="VEL04_02740"
     CDS             3693..>4193
                     /locus_tag="VEL04_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HYT47315.1"
                     /translation="MLIDLCELSPSYVRSIAPYQPGKPIGELAREMGLDEAGIVKLAS
                     NENPRGIGPRTRAAIEAAIGEVARYPDGNGFELKSALAARYAVDMASIVLGNGSNDVL
                     ELVAAAFLGPGRAAVMSQHAFAVYPLATQARGARSIVVPAANYGHDLEAMVRAVDDES
                     YVIWVAN"
BASE COUNT          759 a         1365 c         1415 g          654 t
ORIGIN      
        1 aagcgcaccg cgctcggcga atatccacgc catggccgcg gcgggcaggg cgtgatcgcg
       61 atccagactt cggagagaaa cggcagcctg gtcggcgcgg tgctggtcga cgaccacgac
      121 gaggtgatgc tgatctcgac cggcggggtg ctgatccgca ccggcgtggc gcagatccgc
      181 agccaggggc gctcgaccca gggcgtgacg ctgatcgcgc tcggcgacgg cgaaaagctg
      241 gcgggcctgg agcgcatcga agagggcgag accaacggca acggcgacgg ctcggacggc
      301 gaatcctcgc cgccgcaggt gagccctgcg ccgcccacgc tgcactgaag tcgcgggtct
      361 ggtccctgat gcagcgcatc ttcaatttca gtgccggccc ggcaatgctg ccggccgaag
      421 tgctgtcgcg cgccggcgac gagatgctcg actggcgtgg cagcggcatg ggcgtgatgg
      481 agatgagcca tcgcggcaag caattcatcg gcatcgccgc cgatgccgag cgcgacctgc
      541 gcgagctgct cgccgttccg cagagctgca aggtgctgtt tctccagggt ggcgcgacgc
      601 tgcaattcgc gcaggtgccg atgaacctgc tgcacggcag gggcaaggcc gactacgtct
      661 cgaccggcga gtggtcgaag agggcgatca aggaagcgaa agcgtattgc gacgtgcaca
      721 tcgcggcgag ctcggaggac aagaacttca cttacgcgcc gaagcgctgg aacgtcagga
      781 aagacgcggc gtacgttcac tactgctcga acgagaccat cggcggcgtg gaatttcatt
      841 ctgtgccgta ccaaggcgag gttcccgtgg ttgccgacgc ctcctcgcat ttcctgtcgc
      901 ggccgatcga cgtagcgaaa ttcggcctga tctacgccgg cgcgcagaag aacgtcggcc
      961 cggcgggcct gtcgatcgtg atcgtgcgcg aggacctgat cgggcgcgcc gccaaaggca
     1021 cgccgagcgt catggactac aagctccagg ccgatgccga ctcgatgctc aacacgccgc
     1081 ccagctactc gatctacatc gccgggctgg tattcaagtg gctgaagcag ctcggcggtg
     1141 tcgccgagat cgagaagagg aatatccaga aagcgaagct gctgtacgag tttctcgatg
     1201 cgagcgcatt tttcagcaat ccggtctcgc gcgaagaccg ctccaggatg aacgtgccgt
     1261 ttacgctgaa agatgcaagc ctggatgaaa ccttccttaa aggcgcagcc gagcgcggca
     1321 tggtgcagct caaagggcac cgctcagtcg gcggcatgcg cgcgtcgatc tacaacgcga
     1381 tgcctctcga gggcgtgcag cgcctggtcg cctacatgca ggagttcgag aaaaaacgtg
     1441 gctaaagcga agaagtgcga gatcctggtg atcaaccaga tcgcgcaggt cggcctgaag
     1501 cgcttcccgg ccgagcgcta cgcggtggtg aaggagtcga aggacccctg ggcgatcctg
     1561 gtgcgctcgc aggacctgca ccaatacgag ttcgggcctg cgctgcgcgc gctcggccgc
     1621 gccggcgccg gcgtcaacaa catccccgtg gcggcgctct cccggcgcgg catcccggtg
     1681 ttcaacgccc ccggcgccaa cgccaacgcg gtcaaggagc tggtgctcgc cggcatgctg
     1741 atcgcagcgc gcaatctcgc gcccgccctg gattttgtgc gtgaaatcga cagttctgaa
     1801 aatctcgacc agcgggtcga gagcggcaag aagcagttcg ccggcttcga gctgccggga
     1861 cacacgctgg gggtggtcgg cctgggcaag atcggcagcc tggtggccga cgcggcgatc
     1921 cgcctgggca tgaacgtgct cggctatgac ccgcatatca cggtggaagc cgcctggagc
     1981 ctgccctcgc aggtgcgccg cgccacctcg gtcgacgaga tcctgaagtc gagccacttc
     2041 gtcagcctgc atgtgccgtt cggcgacaag accaggcacc tggtcagcgc gaagaacatc
     2101 gagcagttcc gcagcggcgc ggtgctgctc aatttctcgc gcgaaggcgt ggtcgccgac
     2161 gacgccgtgc tgaaaggcct cgccagccac aagctgcagt ggtacgtcac cgatttcccc
     2221 agcgccgcgc tgatcggccg gcccggcgtg atcgccctgc cgcacctggg cgcatcgacc
     2281 ggcgaagcgg aggacaattc ggcggtcatg gtggtcgatg aggtgcgcga ctatctcgag
     2341 aacggcaacc tgcagaatgc ggtcaacttc ccggatgcgt cgatcgcgcg cggcgcgccc
     2401 tttcgcctcg cgatcgccaa cgccaacgtg ccggacatgc tggggcggat ctcgcacgcc
     2461 ctcgggcggc gcaagatcaa catccacgac atgctgaaca agtcccgggg cgagatggcc
     2521 tacacgctgg tcgacgtcga cagcccggtg ccgggcgagg ctcttgacga gctgtcgaag
     2581 ctcggcgggg tactcgcagt gcgctatctt ccggttgatg agtaacgaca tcgacaggct
     2641 gcgtcgcgag atcgatgcgc tcgacgacga gctcgtcgcg ctgctgaacc gccgcgcagg
     2701 gcttgccgcg aacatcggca gcttgaaaag cggggcgctg gcctatcgtc cggagcgcga
     2761 aagcgagatc ctgcgccggg tcacgaaaca ccagggactg ctttccgccg agcgcatcgc
     2821 cgccgtcttc cgcgaagtga tctcggcctg ccgcgggctc gagcaagcca tgcgtgtcgc
     2881 ctacctcggc cccgagggga cgttcagcga gcaggcggtg cgcaagcatt tcgggcgcgc
     2941 cgtcggggcg ctgcccgccg cctcggtcga cgaggcgttc agacgctgcg aatcgggcgc
     3001 cacccaattc accgtggtgc cggtggagaa ctcgaccgaa ggagtggtcg gccgcacgct
     3061 cgacctgctg ctcgccacgc cgctcaggat ctgcgccgag atcgagctca gggtgcagca
     3121 gaacttgctt tcgaaagaaa atgcattgaa gaagatcaaa cgcatctact cgcattccca
     3181 gtcgctcgcg cagtgcaacg cctggctggc gcagaatctt ccggcagtgg aaaaagtccc
     3241 cgtcgcatcg aatgcagaag ccgcgcagcg cgcggcgaag gagaagggcg cagcggcgat
     3301 cgccggcgag gctgccgcgg aacgctatgg cctcgccgcg ttggcgcgcg ccatcgaaga
     3361 cagtccgaac aacaccacgc gcttcctggt gctcggcaac ctcgatcccg ctccgaccgg
     3421 caacgaccgc acctcgctcg tgatgtcggc cgagaaccgg ccgggcgccg tgcacgcgct
     3481 gctcacgccg cttgccgagc accgcgtcag catgacgcgc atcgagtcgc ggccctcgcg
     3541 cagccgcggc tcgctctggg aatacgtgtt cttcatcgac ctcgagggtc accagcagga
     3601 cgagaaagtc gcggcggcgc tggccggcct gaggcaaaaa gcgccatttt taaaaatctt
     3661 gggctcttat cccgtcgcgg tggcataaag aaatgctgat cgacctgtgt gagctttcgc
     3721 cctcgtacgt gcgctcgatc gcgccgtacc agccgggcaa gccgatcggc gagctggcgc
     3781 gcgaaatggg gctcgacgag gccggcatcg tcaagctcgc ctccaacgag aacccgcgcg
     3841 gcatcggccc gcgcacgcgc gcggcgatcg aggccgcgat cggcgaggtc gcgcgctatc
     3901 ccgacggcaa cggcttcgag ctgaagtcgg cgctcgcggc gcgctatgcc gtcgacatgg
     3961 cctcgatcgt gctcggcaac ggctcgaacg acgtgctcga gttggtggcg gctgccttcc
     4021 tcggaccggg acgcgccgcg gtgatgtcgc agcatgcctt cgcggtgtat ccgctcgcca
     4081 cgcaggcgcg cggcgcgcgc tcgatcgtcg tgcccgcagc gaactacggc cacgacctgg
     4141 aggcgatggt gcgcgccgtg gacgacgagt cctacgtcat ctgggtcgcc aat
//
LOCUS       DASPXW010000114         2077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1422199, whole genome shotgun sequence.
ACCESSION   DASPXW010000114 DASPXW010000000
VERSION     DASPXW010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1422199"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..850
                     /locus_tag="VEL04_02745"
                     /pseudo
     CDS             1..850
                     /locus_tag="VEL04_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
     gene            912..>2077
                     /gene="nuoG"
                     /locus_tag="VEL04_02750"
     CDS             912..>2077
                     /gene="nuoG"
                     /locus_tag="VEL04_02750"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HYT47316.1"
                     /translation="MLKVEVDGRQVEVPHGSTVMDATNKLGVYVPHFCYHRKLSIAAN
                     CRMCLVEIEKAPKPLPACATPVTDGMKVWTHSPAAQKAQNGVMEFLLINHPLDCPICD
                     QGGECQLQDLAVGYGHSNSRYTEEKRVVSRKELGPLVAAEEMSRCIQCTRCVRFGQEV
                     AGIMELGLAGRGENAEIVSFVGRAVESELSGNMIDICPVGALTSEPFRYTARTWELSR
                     RKSVSPHDGLGSNLVVQVKQNRVMRVLPLENEAINECWLSDKDRFSYEGLNSPERLQR
                     PMLKKDGEWQEAEWEAALDFAVSKLQGKALGILASPHATLEELYLTSKLASAADFRLR
                     QADFSADGARQGIPWLGMAIPELEKLDRVLVVGSFLRKEHPLIAHRLRQAAKRGA"
BASE COUNT          372 a          680 c          698 g          327 t
ORIGIN      
        1 ctacgccgcg ggctacctcg gcaagaacat tctgggtgcc gaattttcct atgagctgta
       61 tgcgcatccc ggttacggcg cctacatctg cggcgaggaa accgcgctgc tcgagtcgct
      121 cgagggcaag aagggcatgc cgcgcttcaa gccgccgttc ccggcgagct atggcctgta
      181 cggcaagccc acgaccatca acaacaccga gaccttcgcc gcggtgccgt ggatcgtgcg
      241 ccacggcggc gaggcgttcc tcgcgctcgg gcgaccgaac aacggcggca ccaagctgtt
      301 ctcggtcacc gggcacgtca gccggccggg caactacgag gtgaggctcg gcacgccgtt
      361 cgccaggctg ctcgagatgg cgggcggggt gcgcggcggc caccagctga agggcgtgat
      421 cccgggcggc tcgtcgatgc cggtgctgcc cgccgacatc atgatgaaga ccgacatgga
      481 ctacgactcg atcgccaagg cgggctcgat gctcggctcg ggcgcggtga tcgtgatgga
      541 cgactcgact tgcatggtgc gcgcgctcga gcgcctctcc tacttctact tcgaggagtc
      601 ttgcggccag tgcacgccct gccgcgaagg cacgggctgg ctctaccggg tggtgaaccg
      661 catcgagaac ggccagggcc gcatcgagga cctcgcgctg ctcgacaacg tcgccgacaa
      721 catcgccggc cgcaccatct gcgcgctcgg cgacgccgcg gcgctgccgg tgaagagctt
      781 catcaagcac tacaggagcg agttcgtgca ccacatcgag cacaagagat gtctggttgc
      841 caaaaatatc gagcccagat ggggtcgtag cgaggcgcac cgttcgaaag ccatgcggga
      901 agccgcctag gatgctgaaa gtcgaagtcg acggcaggca ggtcgaggtg ccgcacggct
      961 cgaccgtgat ggacgcgacc aacaagctcg gcgtctacgt gccgcacttc tgctaccacc
     1021 gcaagctctc gatcgctgcc aactgccgca tgtgcctggt cgagatcgag aaggcgccca
     1081 agccgcttcc cgcctgcgcc acgccggtca ccgacggcat gaaggtctgg acccattcgc
     1141 cggcggcgca gaaggcgcag aacggcgtca tggagttcct gctcatcaac catccgctcg
     1201 actgcccgat ctgcgaccag ggcggcgaat gccagctgca ggacctcgcg gtcggctacg
     1261 gccacagcaa ctcccgctac acggaagaga agagggtagt ttcaagaaaa gaattaggtc
     1321 ccctggtcgc tgccgaggag atgagccgct gcatccagtg cacgcgctgc gtgcgcttcg
     1381 ggcaggaggt cgccggcatc atggagctcg gcctcgccgg gcgcggcgag aacgccgaga
     1441 tcgtgtcctt cgtcggccgc gcggtggagt ccgagctctc gggcaacatg atcgacatct
     1501 gtccggtggg cgcgctcacg tccgagccct tccgctacac cgcgcgcact tgggagctgt
     1561 cgcgccgcaa gtcggtctcg ccgcacgacg gcctgggctc gaacctggtg gtgcaggtga
     1621 agcagaaccg cgtcatgcgc gtgctgcccc tcgagaacga ggcgatcaac gaatgctggc
     1681 tgtcggacaa ggaccgcttc tcctacgagg ggctgaactc gcccgagcgc ctgcagcggc
     1741 cgatgctgaa gaaggacggc gagtggcagg aggcggaatg ggaagcggcg ctggacttcg
     1801 ccgtctcgaa gctccagggg aaggcgctgg gcatcctcgc ctccccgcat gccacgctcg
     1861 aggagcttta tttgacttcg aaactggcaa gtgcggctga cttccggctc aggcaagctg
     1921 acttttcggc cgacggcgcg cgccaaggca ttccctggct cggcatggcg atcccggagc
     1981 tcgagaagct cgaccgcgtg ctggtcgtcg ggtcgttcct gcgcaaggag catccgctca
     2041 tcgcgcaccg gttgcgccag gcggcgaagc gcggcgc
//
LOCUS       DASPXW010000115         3074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1672466, whole genome shotgun sequence.
ACCESSION   DASPXW010000115 DASPXW010000000
VERSION     DASPXW010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1672466"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="VEL04_02755"
     CDS             complement(<1..909)
                     /locus_tag="VEL04_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HYT47317.1"
                     /translation="MSVATQGLVRLPVWRARIVLAALAAAFGVLTVRSLYLQAMKTDF
                     LQEKGDARYSRVLEVPATRGRILDRNGEALAVSTPVKSVWAIPADVELSRTQRKKLAT
                     LLTLEPAELDRKLADASRDFVYLKRQVSPDTAQAVAALGVKGIYQHPEYRRYYPGGEV
                     TAHVIGFIGVDGGGQEGVELAYEATLAGKAGSRRVIKDRLGGIVEDVEAIRAAQDGTD
                     LRLSIDSKLQSLAYAALKGAVEAHRARAGGVVALDVRTGEILALVNVPSYNPNNRARL
                     TGAQIRNRVITDLFEPGSTLKPFTIAL"
     gene            complement(906..1178)
                     /gene="ftsL"
                     /locus_tag="VEL04_02760"
     CDS             complement(906..1178)
                     /gene="ftsL"
                     /locus_tag="VEL04_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HYT47318.1"
                     /translation="MAKLNLLLLAVLVACALGLVTSQHKARKLFSELEREHDRARELD
                     VEYGRLQLEASTWGLHSRVEKIATGALGMRPADARRVRIVGRDAGR"
     gene            complement(1166..2128)
                     /gene="rsmH"
                     /locus_tag="VEL04_02765"
     CDS             complement(1166..2128)
                     /gene="rsmH"
                     /locus_tag="VEL04_02765"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006163490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HYT47319.1"
                     /translation="MHQPVLEQEAMQALAIRPDGIYVDGTFGRGGHSRALLARLGPRG
                     RLVAFDRDPEAMTAAGAIPDARFSFVRSNFGALNQALDQAGVGLAHGMLFDLGVSSPQ
                     LEDAARGFSFRADGPLDMRMDPAHGVSAAQWLAAAEEQEIREVIRSYGEERFAQQIAA
                     AIVAARRRQPLLRTRQLADLVAQAVRTREPGQDPATRTFQAVRIHVNRELEEVSLMLP
                     QAVARLAPGGRLVVISFHSLEDRLVKRFMQGAARPDVPREVPLRQAEMPQPALRLVGR
                     AMRASQQELEANPRARSATLRVAERTAAPHSPNIRIGPGARWRN"
     gene            complement(2138..2587)
                     /gene="mraZ"
                     /locus_tag="VEL04_02770"
     CDS             complement(2138..2587)
                     /gene="mraZ"
                     /locus_tag="VEL04_02770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HYT47320.1"
                     /translation="MGTAFHGAAVITLDAKGRVAIPTRHRSALLDAGKSLVVTAHPDG
                     CVLIYPAAEWEPVRARVEAFPSFHPQASWWKRLLLGFEEHVAPDSSGRILLPSALRVH
                     AKLERDAMMVGQGRYFELWDSGVWAAKLGQALAGPTAPPPGMEDFSL"
     gene            2803..>3074
                     /locus_tag="VEL04_02775"
     CDS             2803..>3074
                     /locus_tag="VEL04_02775"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HYT47321.1"
                     /translation="MIDKFFPSAEAALADIADGATIMIGGFGNAGMPEALIDALIAQG
                     AHELTIVNNNAGNADTGLAALLKAKRVRRILCSFPRQADSWHFDAL"
BASE COUNT          518 a         1041 c          988 g          527 t
ORIGIN      
        1 gagtgcaatc gtgaatggct tgagcgtcga gccgggctcg aacagatcgg tgatgacgcg
       61 attgcgaatc tgcgcccccg tgagccgcgc gcggttattg gggttgtacg atggcacgtt
      121 gacgagggcg aggatctcgc cggtgcgcac atcgagcgcc acgaccccgc cggcccgggc
      181 gcggtgggcc tcgaccgcgc ctttcagggc ggcgtaggcc aggctctgca gcttgctgtc
      241 gatcgacagc cgcaggtcgg tgccgtcctg cgccgcgcga atcgcctcga cgtcctcgac
      301 gatgccgccg aggcggtcct tgatgacgcg ccggctgccc gccttgccgg caagcgtcgc
      361 ctcgtacgcg agctcgacgc cttcctgccc gccaccgtcg acgccgatga atccgatcac
      421 gtgcgccgtc acttctccgc ccggatagta gcggcggtat tcgggatgct ggtagatgcc
      481 cttcacgccg agcgccgcaa ccgcctgcgc ggtgtcgggc gagacctggc gcttgagata
      541 gacgaaatcg cgcgaggcat cggcgagctt gcgatcgagc tcggctggct caagggtgag
      601 cagcgtggca agcttttttc tttgcgtgcg ggacagctcg acgtcggcgg ggatcgccca
      661 caccgatttc accggcgtcg acactgcgag cgcttcgccg ttgcggtcga ggatgcgacc
      721 gcgcgtcgcc gggacctcga gcacgcggga gtaacgcgcg tcgcccttct cctgcaggaa
      781 gtcggtcttc atcgcctgga gatagagcga gcgcacggtg agcacgccga aggccgccgc
      841 gagcgccgca agcacgatgc gcgcgcgcca gaccggcagc ctgaccaggc cctgcgtcgc
      901 cacgctcacc ggcccgcgtc tcgaccgacg attcgcacgc ggcgcgcgtc cgcgggccgc
      961 atgccgagcg ccccggtggc gatcttctcg acgcgcgaat gcaggcccca ggtgcttgcc
     1021 tcgagctgca ggcgcccgta ttcgacatcg agctcgcggg cgcgatcgtg ctcgcgctca
     1081 agctctgaga acagtttgcg cgccttgtgc tgagaagtga cgagcccgag cgcgcaggcg
     1141 acgagaaccg cgaggaggag gaggttcagt ttcgccaccg cgcgccgggc ccgattcgga
     1201 tgttggggga gtgaggcgcc gcggtgcgct cagccaccct caaggtggcg ctgcgtgccc
     1261 gcgggtttgc ttcgagttct tgctgtgatg cgcgcatggc gcggccgacg agcctgagcg
     1321 caggctgcgg catctcggcc tgccgcagcg gcacttcgcg cggcacatcg gggcgcgccg
     1381 cgccctgcat gaagcgcttg acgagacggt cttccagcga gtgaaagctg atcaccacga
     1441 gccggccgcc gggcgccagc cgcgctaccg cctgaggcag catcagcgac acttcctcaa
     1501 gctcccgatt gacgtgaatc cgtacagcct gaaaggtgcg cgtcgccggg tcctggcccg
     1561 gctcgcgtgt gcggaccgcc tgagccacga gatcggcaag ttgccgggtg cggaggagtg
     1621 gttggcggcg gcgagcagca acaatcgccg ctgcaatctg ttgagcaaac cgttcttcgc
     1681 cataggaccg gatgacctcc ctgatttctt gttcttctgc cgccgcgagc cattgcgccg
     1741 ccgacacgcc gtgcgccgga tccatgcgca tgtcgagcgg gccgtcggcg cgaaaggaga
     1801 agccgcgcgc tgcatcctcg agctgcggcg aggacacgcc cagatcgaac agcatgccgt
     1861 gcgccagacc taccccagcc tgatcgagcg cttggttcag cgccccgaaa ttgctcctca
     1921 cgaatgaaaa ccgcgcatcg ggaatcgccc cggcagcagt cattgcctcg ggatcgcggt
     1981 cgaacgccac cagccgcccg cgcggcccga gccgcgcgag cagcgcgcgg ctgtgcccgc
     2041 cacgtccgaa cgtgccgtcg acgtagattc cgtccggccg gatcgcgagc gcctgcatcg
     2101 cctcctgctc cagcaccggc tgatgcatga tcggcgctca cagcgagaaa tcctccatcc
     2161 cgggaggcgg tgcagtcgga ccggcgagtg cctgcccgag cttcgccgcc cacacgcccg
     2221 agtcccacag ctcgaaatag cggccctgcc cgaccatcat cgcatcgcgc tcgagcttgg
     2281 cgtgtacgcg cagcgcgctc ggcagcagaa tccgcccgga actgtcgggc gcaacgtgct
     2341 cctcgaatcc gagcagcagc cgcttccacc aactcgcctg cggatgaaag ctcggaaatg
     2401 cctctacccg agcgcgtacc ggctcccatt ccgctgccgg atagatcagc acgcagccgt
     2461 ccggatgcgc cgtgacgacg agcgacttgc ccgcatcaag cagcgcggag cgatggcgcg
     2521 tgggaatagc gacgcgtccc tttgcgtcga gcgtgatgac cgccgctcca tgaaatgctg
     2581 tccccatatg gcttgtgcca cttttttcta ccttttacca ctctaatctt aacagatatg
     2641 gcgggtcaag ccttttttct tgccgggaca acgacttagc atatttttag gaggcgattt
     2701 ctcgctgttt tgatgttata taaatcaaat acttatttta ttaagtaaaa gtcacttatt
     2761 aacagaaagc ggctggaacg cgatgcgata atttctgccc ccgtgatcga caagttcttc
     2821 cccagcgccg aggctgcgct cgccgatatc gccgacggtg caacgatcat gatcggcggc
     2881 tttggcaacg ccggcatgcc ggaggcgctg atcgacgcgc tgatcgccca gggcgcccac
     2941 gagctgacca tcgtcaacaa caacgccggt aacgccgaca ccgggcttgc ggcactgctc
     3001 aaggccaagc gcgtgcgcag gatcctgtgc tcgttcccgc gccaggccga ctcctggcat
     3061 ttcgatgcgc tcta
//
LOCUS       DASPXW010000116         2918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1672516, whole genome shotgun sequence.
ACCESSION   DASPXW010000116 DASPXW010000000
VERSION     DASPXW010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1672516"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..2003
                     /locus_tag="VEL04_02780"
     CDS             144..2003
                     /locus_tag="VEL04_02780"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HYT47322.1"
                     /translation="MNAPAPAHADQSNAPSWVRHQALRRWVAEVARLAKPERIVWCDG
                     SQSEYDRLCDELVEAGTFTRLNAKLRPDCYLARSHASDVARMEDRTFICSRSKDDAGP
                     TNNWVEPREMRATLERLFDGCMRGRAMYVIPFSMGPIGSHIAHIGVEISDSAYVAVSM
                     RIMTRMGRAVVEWLGETGKFVPCVHSVGAPLAVKPGARIKDAAWPCNEDEKWIVHFPE
                     TREIWSYGSGYGGNALLGKKCLALRIASVMARDEGWLAEHMLILGIESPAGEKSYVAA
                     AFPSACGKTNLAMLIPPSAFKGWKVTTIGEDIAWIKPGRDGRFHAINPEYGLFGVAPG
                     TSEHTNLNAMRMLRENVIFTNVAMTPEGDVWWEGMTDEPPARLIDWQGRPWTPADGKR
                     GRLAAHPNARFTVAAAQCPSMDSKWEDPEGVPISAFVFGGRRSSTVPLVVEAPSWEEG
                     VYMAATLGSETTAATTGKVGVVRRDPMAMLAFCGYNMGDYFAHWLKMGRTVQPPPRIF
                     RVNWFRRDAQGKFIWPGFGENMRVLQWIVERCQSRSRGADTPLGTMPRFEDLNWSGLE
                     TLSPAQYAELTRIDAASWKDELVSHDEFFGKLGAHLPAALEARRGRMRERLAA"
     gene            2162..>2918
                     /locus_tag="VEL04_02785"
     CDS             2162..>2918
                     /locus_tag="VEL04_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYT47323.1"
                     /translation="MSFPSEFDVIVIGGGHAGTEAALASARLGCRTLLLTHSIETLGQ
                     MSCNPSIGGIGKGHLVKEIDALGGAMAITTDEAGIQFRTLNSSKGPAVRATRAQADRV
                     LYKQAIRGRLEQQSNLSLFQQAVDDIEVVGDRVSAVVTQLGLRFRGRAVVLTAGTFLA
                     GRVHVGLENYQAGRAGDPASITLAARLREMRLPVGRLKTGTPPRLDGRSIDYSQLEMQ
                     PGDAPEPVFSFLGRRDMHPRQLPCWIAHTNERTH"
BASE COUNT          503 a          906 c          984 g          525 t
ORIGIN      
        1 ttgtacttac atgtttttat ctgttcggct cacccctcgg gagacctgct ttctcgagct
       61 tgggtgctaa aattcgatgc gacacagtcc ctatccgacc ggcccagccc cgctgggccg
      121 tttgcctttt tggagtgaga ccaatgaatg ctccagcacc ggctcatgcc gaccaatcca
      181 acgcgccgag ttgggttcgc catcaagcgc tgcggcgttg ggtggcggag gtggcccggc
      241 ttgccaagcc ggaacgcatc gtctggtgcg acggttcgca gagcgagtat gaccggttgt
      301 gcgatgagtt ggtggaggcg ggaaccttca cgcgcctgaa cgcgaagctg cgccctgact
      361 gctacctcgc gcgctcgcac gcgagcgacg tcgcccgcat ggaagaccgc actttcatct
      421 gcagccggtc gaaggacgac gccgggccga cgaacaactg ggtcgagccg cgcgaaatgc
      481 gcgccacgct cgagcgccta ttcgacggtt gcatgcgcgg gcgcgcgatg tacgtaattc
      541 cattttcgat ggggcccatc ggctcgcaca tcgcgcacat tggcgtcgag atctccgact
      601 cggcgtacgt cgcggtgagc atgcggatca tgacgcgcat gggacgcgca gtcgtcgagt
      661 ggctcggcga aaccggcaag ttcgtgcctt gcgtccattc ggtcggcgcg ccgctcgcgg
      721 tcaagcccgg cgcgcgcatc aaggacgcag cctggccgtg caacgaggac gaaaagtgga
      781 tcgtgcattt tcccgagacg cgcgagatct ggtcgtacgg ctcgggctac ggcggcaatg
      841 cgctgcttgg aaagaaatgc ctggcgctgc gcatcgcctc ggtgatggcg cgcgacgagg
      901 gctggctcgc ggagcacatg ctgattctgg gcatcgagtc gcctgcggga gagaagagct
      961 atgttgccgc cgcgttccct tctgcgtgcg gcaaaaccaa tctcgcgatg ctgattccgc
     1021 cctccgcgtt caagggctgg aaggtgacca cgatcggcga ggacatcgcc tggatcaagc
     1081 cggggcgcga cggtcgcttc catgcgatca atccggaata cggcctgttc ggcgtagcgc
     1141 ccggaacctc cgagcacacc aacctgaatg cgatgcgcat gttgcgcgag aacgtcatct
     1201 tcacgaatgt cgcgatgacg cccgagggcg atgtctggtg ggaaggcatg accgacgagc
     1261 cgccggcgcg cctcattgac tggcaaggca gaccctggac gcctgcggac ggcaagcggg
     1321 gcaggctcgc tgcgcatccc aacgcgcgct tcaccgttgc ggcggcgcaa tgcccgtcga
     1381 tggattcgaa atgggaggat ccagaagggg tgccgatctc tgcgttcgtc ttcggcggcc
     1441 gacgctcgtc gacggtaccg ttggtggtgg aagcgccgag ctgggaagaa ggcgtttaca
     1501 tggcggcaac gctcggttcc gagactaccg cggcaaccac cggcaaggtc ggcgtggtga
     1561 ggcgcgaccc gatggcgatg ctggcattct gcggctacaa catgggcgac tatttcgccc
     1621 attggctgaa gatgggccgc accgtgcagc cgccgccgcg gattttccgc gtcaactggt
     1681 tccggcgcga cgcgcagggc aagttcatct ggcccgggtt tggcgagaac atgcgcgtgc
     1741 tgcagtggat cgtcgagcgc tgtcagagcc gctcgcgcgg cgccgacacg ccgcttggca
     1801 cgatgccgcg tttcgaggac ctgaactgga gcggtctgga gacgctctcg cctgcgcagt
     1861 acgccgagct gacgcgcatc gacgcggcgt catggaagga cgagcttgtc tcgcacgatg
     1921 aattcttcgg caagctcggc gcgcacctgc cggcggcgct ggaggcgcga cgcgggcgca
     1981 tgcgcgagcg gctcgcagcc tgaggcgccg ctagagccct tcccccagcg tagggttacg
     2041 aaaggcggcg attcccccac ggcgcctggt gatgctgcgc tgcagcaaca agtctttgtt
     2101 ccacgtgaaa caagggtgaa actggtttca cgtggaaccg tcgcgtaaaa ttcgcagccc
     2161 gatgtctttc ccgagcgaat tcgacgtcat tgtcattggc ggtggccatg ccggcaccga
     2221 ggccgcgctc gcttcggccc gcctgggctg ccgcaccctg cttttgaccc acagcatcga
     2281 gaccttgggg cagatgtcct gcaatccatc cattggcggc atcggcaagg gccatttggt
     2341 caaagagatc gacgccttgg gtggagccat ggcgatcacc actgacgagg ccgggatcca
     2401 gttccgcacg ctcaattcat ccaaaggacc ggcggtgcgt gccacccgcg cgcaggccga
     2461 tcgcgtgctt tacaagcagg cgattcgcgg gcggctagag cagcagtcga acctgagtct
     2521 gttccagcag gcggttgacg acatagaggt ggtgggcgat cgcgtgagtg cggtcgtgac
     2581 gcagcttggc ctgcgctttc gaggccgggc cgtagtgctg acggcaggta cctttctcgc
     2641 ggggcgcgtt catgtcggcc tcgagaacta ccaagccggt cgcgccggcg atccggcatc
     2701 gatcacgctc gccgcgcggc tgcgggagat gcgattgccg gtcggccggc tgaaaaccgg
     2761 gaccccgccg cgactcgacg ggcgtagcat cgattattcg cagctcgaga tgcagcccgg
     2821 cgacgcgcct gagccggtgt tttcattttt ggggcggcgg gacatgcacc cgcgccaatt
     2881 gccgtgctgg attgcgcaca ccaacgagcg cacccacg
//
LOCUS       DASPXW010000117         3402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_36223, whole genome shotgun sequence.
ACCESSION   DASPXW010000117 DASPXW010000000
VERSION     DASPXW010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36223"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /locus_tag="VEL04_02790"
     CDS             complement(<1..1096)
                     /locus_tag="VEL04_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HYT47324.1"
                     /translation="MSTPFSLSLAAIVALSLLGLPIGHAMIAGSILYLYAAGLDMGTA
                     AEQLLNGMYSSYLLMAIPLFILAAEFMNSGSIMDRLLRFCNAMVGHFRGGLAHVNVVQ
                     SIIFASMSGSALADAAGSGKLMQSLMTRDGKYTASFAAALTAVSSVIGPIIPPSIPLV
                     LYALVSDTSIGYLFLGGVIPGLLIGAVQMALIAFTAKKNNFPVEAAVPMREWPRVTRE
                     AFPALMMPVILLTLLYSGVTTPTEAAAAAAAYALLVSAILYRSITWSDVYRSLATSAR
                     ITVSIGMLIAGALVFNYVVTIENIPKTLSAMLTASHMSPLMYLMFVNVLLLILGCLLE
                     GTTIILIILPVLLPTATALGIDPVHFGVVAV"
     gene            complement(1093..1605)
                     /locus_tag="VEL04_02795"
     CDS             complement(1093..1605)
                     /locus_tag="VEL04_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HYT47325.1"
                     /translation="MSLQKAGAWLHRRAENIAAALLAVMFIAFIVQIVFRYLFNFPIG
                     WSTELTIVTWLYLVLWGSAFVLTEKEEIRFDLIYGSVGRRGRSAMAIVFSVSVVVAYG
                     AALPATYAYVSFMKVEKSSYLKIPLNWLYSIYVVFCVAMIVRYLWLLWQLVRGRDPEA
                     ADLTKVSSGL"
     gene            complement(1613..2605)
                     /gene="dctP"
                     /locus_tag="VEL04_02800"
     CDS             complement(1613..2605)
                     /gene="dctP"
                     /locus_tag="VEL04_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HYT47326.1"
                     /translation="MDFKIDRRSALKAGAALAAASTLPAWAQAKPTLRFAAVFSDKDI
                     RADMVRMLAKDVGGDYSVETFFNASLFKQGTELVALQRDNLEMGNIAPQDISKQVPAW
                     TILTSAYLFRDADHLSKFWSGDLGAQFKKQAEEQVKIKILGPTFFGTRQVGLKVKKKI
                     NTPTDMAGIKLRMPPGDAWQLLGRSLGANPTPMAFAETYTGLQTGAVDGQDNPLPNVN
                     AVKFYEVMSQIVLTGHLIGYDLLSMNLKTWNAMSPARQKAFQAAVDKALAWSTAEHLK
                     REAELADIFRKQHGLEIYTPDVNAFRTYAQKVYLASDEAKDWPKGMLEKINALK"
     gene            complement(2639..3259)
                     /locus_tag="VEL04_02805"
     CDS             complement(2639..3259)
                     /locus_tag="VEL04_02805"
                     /inference="COORDINATES: protein motif:HMM:NF037007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HYT47327.1"
                     /translation="MRKPKAPEANRASIVRAAIDEFAARGFKGASMDAIAARTHTTRA
                     LINYYFGSKEKIYLAVLEHVYSEIREAESRLDLDHLPPAEAIRRIVEFTFDYYLDHEG
                     FVRLVVAENQARGRHLKKSKAMRTLNRPVIETLARVIERGRRDGSFGRDVDPVEIHKA
                     IAALGMFNVTNQYTFGAIFQRDMGTKGDVRRRREMVAEVILSYLSA"
BASE COUNT          626 a         1053 c         1129 g          594 t
ORIGIN      
        1 ccacggcgac cacgccgaag tgcactgggt cgatgccgag cgccgtcgcg gtcggcagca
       61 gcaccggcag gatgatgagg atgatcgtcg tgccttccag caggcagccg aggatcagca
      121 gcaggacgtt tacgaacatc aggtacatca gcggcgacat atgcgacgcc gtcagcatgg
      181 cgctgagcgt cttcggaatg ttctcgatcg tcacgacgta gttgaacacc agcgcgccgg
      241 cgatcagcat gccgatcgag accgtgatgc gcgcgctcgt tgccagcgag cggtagacgt
      301 cgctccaggt gatgctgcga tagagaatcg ccgagaccag cagcgcgtag gcggcagcgg
      361 ccgccgcggc ctcggtgggc gtggtcaccc cgctatagag cagggtcagc aggatcaccg
      421 gcatcatcag cgccgggaag gcctcccggg tgacgcgcgg ccattcgcgc atcggaacgg
      481 ccgcctcgac cgggaagttg ttcttcttgg cggtaaacgc gatcagcgcc atctgcacgg
      541 cgccgatcag gagccccggg atgacgccgc cgaggaacag gtaaccgatg gaggtgtcgg
      601 agaccagggc gtacagcacc agagggatgg agggcgggat gatcggcccg atcaccgaag
      661 agaccgccgt caacgccgcg gcgaagctcg cggtgtactt gccgtcgcgc gtcatgagcg
      721 actgcatcaa cttgcccgag cccgccgcgt ccgcgagggc ggagcccgac atgctggcga
      781 agatgatgct ctgcactacg ttgacgtgcg cgaggccgcc gcggaagtgc ccgaccatcg
      841 cgttgcagaa gcgcagcagg cgatccatga tgctgccgct gttcatgaac tcagcggcga
      901 ggatgaagag cggaatcgcc atcagcaggt agctcgagta catgccgttc agcagctgct
      961 cggcggcggt gcccatgtcg aggccggccg cgtacaggta gaggatcgat ccggcgatca
     1021 tcgcgtggcc gatcggcagg ccgagcaggc tcagggccac gatcgccgcg agggagagcg
     1081 agaagggggt gctcacaggc ccgagctcac cttggtcaga tcggccgctt caggatccct
     1141 gccgcgcacg agctgccaca gcaaccagag gtagcgcacg atcatcgcga cgcaaaacac
     1201 cacgtagatg gaatacagcc agttgagggg gatcttgaga taggaggact tctcgacctt
     1261 catgaagctg acgtacgcgt acgtcgccgg cagcgcggcc ccatacgcga ccacgactga
     1321 taccgagaag acgatcgcca tggcgctgcg cccgcggcgg cccaccgacc cgtagatgag
     1381 gtcgaagcgg atctcctcct tctccgtaag gacgaaagcc gaaccccaca gcacgaggta
     1441 aagccacgtg actatggtca gctcggtgga ccagccgatc gggaagttga acaggtagcg
     1501 gaaaacgatc tgcacgatga aggcgatgaa catcaccgcg agcagcgccg cggcgatgtt
     1561 ctcggcccgg cggtgcagcc aggcgcccgc tttctgcaac gacaccccgg ggctacttca
     1621 gggcgttgat cttctcgagc atgcccttcg gccagtcctt cgcctcgtcg gatgccaggt
     1681 agaccttctg cgcatacgtg cggaaggcgt tgacgtccgg ggtatagatc tcgaggccgt
     1741 gctgcttcct gaagatatcg gccagctccg cctcgcgctt caggtgctcc gccgtgctcc
     1801 aggccagcgc cttgtccacc gcggcctgga acgccttctg ccgcgccggc gacatcgcgt
     1861 tccaggtctt caggttcatc gacagcaggt cgtatccgat caggtggcca gtgagcacga
     1921 tctgcgacat cacttcgtag aacttgaccg cgttgacgtt gggcagcggg ttgtcctggc
     1981 cgtccactgc gccggtctgc aggcccgtgt aggtctcggc gaaggccatc ggcgtcggat
     2041 tcgcgccgag cgagcgcccg aggagctgcc aagcgtcgcc cggaggcatc ctgagcttga
     2101 tgccggccat gtcggtgggc gtattgatct ttttcttcac cttcaggccc acctgccgcg
     2161 tgccgaagaa ggtcggtccc aggatcttga tcttcacctg ctcctcagcc tgcttcttga
     2221 actgcgcgcc gaggtcgccc gaccagaact tgctcagatg gtcggcgtcg cggaaaagat
     2281 acgccgaggt gaggatggtc cacgccggga cctgcttgga gatgtcctgc ggcgcgatgt
     2341 tgcccatctc gaggttgtcg cgctgcaagg cgacgagctc ggtgccctgc ttgaaaagcg
     2401 acgcattgaa gaacgtctcg acgctgtaat cgcctccgac atccttggcg agcatgcgga
     2461 ccatgtccgc gcggatgtcc ttgtctgaga acaccgccgc aaagcggagg gtgggcttgg
     2521 cctgcgccca ggcgggcagg gtagatgcgg cagcgagggc ggcgcccgcc ttgagtgcgg
     2581 aacgacgatc gattttgaag tccatgctat tcctcctcct atgaagaagc gttgacagct
     2641 aagcgctcag gtagctgaga atcacttccg cgaccatttc gcggcgtcgg cgcacgtctc
     2701 ccttggtgcc catgtcccgc tggaagatgg cgccgaacgt gtactggttg gtgacgttga
     2761 acatgccgag cgcggcgatg gccttgtgga tctccaccgg gtcgacgtcg cgcccgaagc
     2821 tgccgtcgcg ccggccgcgc tcgatgacgc gcgctagggt ctcgatcacc ggacggttga
     2881 gcgttcgcat ggctttcgat ttcttcaagt ggcggccccg cgcctggttc tcggcgacca
     2941 ccaggcgcac gaaaccctcg tggtcgaggt agtagtcgaa ggtgaactcc acgatgcgcc
     3001 ggatcgcctc ggccggcggc aggtgatcga ggtcgagccg gctctccgct tcgcggatct
     3061 ccgaatacac gtgctcgagg accgccaggt agatcttctc tttgctgccg aagtagtagt
     3121 tgatcagcgc ccgcgtggta tgcgtgcgcg ccgcgatggc gtccatgctc gcgcccttga
     3181 agccgcgcgc agcgaactcg tcgattgccg cccggacgat ggaggcgcgg ttggcctcgg
     3241 gcgccttggg cttgcgcatg gcagcccagc cggaagatcg ggcgcgcgac ggggggatgg
     3301 cgccggtcgc tttcattgtg tagggtctgg aattgacgaa cctgcaggaa ttaacgaacc
     3361 tgcaaatttc aatttaacag agccgcctgc aaatgactag cg
//
LOCUS       DASPXW010000118         2757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_412921, whole genome shotgun sequence.
ACCESSION   DASPXW010000118 DASPXW010000000
VERSION     DASPXW010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412921"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..549
                     /locus_tag="VEL04_02810"
     CDS             49..549
                     /locus_tag="VEL04_02810"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HYT47328.1"
                     /translation="MTAAIRIAFAGLTCALAGTFAAPASAQTVTHKDISVDAAVTIAT
                     TAMADCKAKGWPVSVAVVGRSGELLVHMRGDGTGPHTMDNSFRKAYTSRTFRIPSGEM
                     VERLKANPQLGLIHLNNVIANQGALPIKVGEDTIGAAGASGAPGGEKDEACVKAGIDK
                     VAGMLK"
     gene            570..1874
                     /gene="hflX"
                     /locus_tag="VEL04_02815"
     CDS             570..1874
                     /gene="hflX"
                     /locus_tag="VEL04_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HYT47329.1"
                     /translation="MQEPGQKRTRAIVAAVQRSSVSDAEFDASLTELRQLAKTLGFEV
                     VGRFTQKRAGFDKTAYFGTGKREELKEQILENHAEIILIDHEISPSQSHNLEKEVGCE
                     VMDRTMVILEIFHRHASSRFARAQVEIARLGYMGPRLREAARQAGPKDRARSGTGGRS
                     GGESHGELDRRKIRDRITELQKELDGMALERKTQRARRLERQGLARVALVGYTNAGKS
                     TLMRALTGNEALVADKLFATLDTTVRALHPESIPRVLVSDTVGFIKNLPHGLVASFKS
                     TLDEALEASLLAHVVDASDPGFERQLAVTEAVLDEIGAQEVPRLLVFNKIDRGGDDAT
                     LHAKYPGCIVMSAKREGDIAKLREAINAFFQRRLVEAELFLPWSAQNLRGQIFATCTV
                     IEERSGDAGAFLRVRGEPEAVERLIAQFGEQFGRVDSPSSGS"
     gene            1882..>2757
                     /locus_tag="VEL04_02820"
     CDS             1882..>2757
                     /locus_tag="VEL04_02820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HYT47330.1"
                     /translation="MAQKKTALQTLELQLREAQSLAHFGSWEWDLREDRVTWSEALYR
                     MMGVDPATFRPSVDAFLGCVHPEDRERVARAAAESRASGLPYTAPLRIVRPDGSVRTV
                     RGGSHAVTDAAGHPVRVVGILQDISDETGAQEALRRYAEQVVVLARRLVEVQEIQGRR
                     LARELHDRLGATLTALSINLRLIEDALPPALRQGLADTLADSRGQIDEASVAMRDVIG
                     ELRPHELDDHGLPAALRALAAGFEKRTGIRTAVSVNASERAIDGRELPLYRIAQEALN
                     NVVKHSRAPSVRIRYA"
BASE COUNT          469 a          953 c          956 g          379 t
ORIGIN      
        1 ccttccttgc atgccgccgg acagagcggc cccaagggag gatcattcat gactgctgcc
       61 attcgtattg cgtttgccgg cctgacctgc gctctggccg gcacgttcgc cgctccggca
      121 tcggcacaga cggtcacgca caaggacatc tcggtcgacg ccgcggtgac catcgccacc
      181 accgcgatgg ccgactgcaa ggccaagggc tggccggtgt cggtggccgt ggtcggccgc
      241 agcggcgaac tgttggtgca catgcgcggc gacggcaccg gcccgcacac gatggataac
      301 agcttccgca aggcctatac ctcgcgcacc ttccgcatcc cgtcgggcga gatggtggag
      361 cggctcaagg ccaacccgca gctcggcctg atccacctca acaacgtgat cgcaaaccag
      421 ggcgcgctgc cgatcaaggt gggcgaggac accatcggcg cggccggcgc ctccggcgcg
      481 ccgggcgggg agaaggacga ggcctgcgtc aaggccggca tcgacaaagt cgccggcatg
      541 ctgaagtagt tccgcccggg ccgcaggtga tgcaggaacc cggccagaag cggacgcgcg
      601 ccatcgtagc ggccgtgcag cggtccagcg tgagcgatgc cgagttcgac gcatcgctga
      661 ccgagctgcg ccagctcgcg aagacgctgg gcttcgaggt cgtcggcaga ttcacgcaga
      721 agcgcgccgg cttcgacaag acggcctact tcgggaccgg taaaagggaa gaacttaaag
      781 aacaaatttt agaaaaccac gccgaaatca tcttgatcga ccacgagatc tccccgtcgc
      841 agtcgcacaa cctggagaag gaggtcggct gcgaggtgat ggaccgcacc atggtgatcc
      901 tcgagatctt ccaccggcac gcgagctcgc gcttcgcgcg cgcgcaggtc gagatcgccc
      961 gcctgggcta catggggccg cgcctgcgcg aagccgccag gcaggccggg ccgaaggacc
     1021 gggcgcgcag cggcaccggc ggccgcagcg gcggcgagtc gcacggcgag ctcgaccggc
     1081 gcaagatccg cgaccgcatc accgagctgc agaaggagct cgatggcatg gccctcgagc
     1141 gcaagacgca acgcgcccgg cgccttgagc gccaggggct cgcgcgcgtg gcgctcgtcg
     1201 gctacacgaa cgcgggcaag tccacgctga tgcgcgcgct gacgggaaac gaggcgctgg
     1261 tcgccgacaa gctcttcgcg acgctcgaca ctacggtgcg cgccctccac cccgagagca
     1321 tcccgcgcgt gctggtcagc gacacggtcg gcttcatcaa gaacctgccg cacgggctcg
     1381 tcgcatcgtt caagtcgacg ctcgatgaag cgctcgaggc gtcgctgctc gcccacgtcg
     1441 tcgatgcgag cgatccaggg ttcgagcggc agctcgcagt gaccgaggca gtgctcgacg
     1501 agatcggcgc gcaggaggtg ccgcgcctcc ttgtgttcaa taagatcgac cgcggtgggg
     1561 atgatgcgac gttgcatgcc aagtaccccg gctgcatcgt gatgagcgcg aaaagggagg
     1621 gcgacatcgc gaagctgcgc gaggcgatca acgcgttctt ccagcggcgc ctcgtcgagg
     1681 ccgagctgtt cctcccctgg tcggcgcaga acctgcgcgg gcagatcttc gccacctgca
     1741 cggtgatcga ggagcgctcc ggcgacgccg gagcattcct cagggtgcgc ggcgagcccg
     1801 aggccgtgga gcgcctcatt gcacaattcg gcgagcagtt cggccgggta gactcccctt
     1861 cctctggaag ctgaatcggg tatggctcaa aagaaaaccg ccctgcagac gctcgagctg
     1921 cagctgcgcg aggcgcagtc gctggcgcat ttcggcagct gggaatggga cctgcgcgag
     1981 gaccgggtga cctggtcgga ggcgctgtac cgcatgatgg gggtcgatcc cgcgaccttc
     2041 cggccctccg tcgacgcatt cctgggctgc gtgcatcccg aggaccggga gcgcgtcgcg
     2101 cgcgcggccg cagagtcgcg cgcgtcgggc ctgccctaca cggcgccgtt acgcatcgtg
     2161 cgccccgacg gcagcgtgcg caccgtacgc ggcggctcgc acgcggtgac cgacgccgcc
     2221 gggcacccgg tgcgcgtcgt cggcatcctc caggacatct ccgacgagac cggggcgcag
     2281 gaagcgttgc gccgctacgc cgagcaggtg gtggtgctcg cgcgccggct ggtcgaggtg
     2341 caggaaatcc agggccgccg cctcgcccgc gagctgcacg accgccttgg cgcgaccctc
     2401 accgcgctca gcatcaacct gcggctcatc gaggacgcac tgccgcccgc gctgcggcag
     2461 ggactcgccg acacgctggc ggattcgcgc gggcagatcg atgaggcctc ggtggcgatg
     2521 cgcgacgtga tcggggagct gcgcccgcac gagctcgacg accacggcct gcccgccgcc
     2581 ctgcgcgcgc tcgccgccgg attcgagaag cgcaccggga tccgcacggc ggtgtcggtg
     2641 aacgcctccg agcgcgcgat cgacggccgc gagctgccgc tctaccgcat cgcgcaggag
     2701 gcgctcaaca acgtcgtcaa gcactcgcgc gccccgagcg tgcgcatccg ctacgcc
//
LOCUS       DASPXW010000119         3281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_916147, whole genome shotgun sequence.
ACCESSION   DASPXW010000119 DASPXW010000000
VERSION     DASPXW010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916147"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VEL04_02825"
     CDS             complement(<1..706)
                     /locus_tag="VEL04_02825"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HYT47331.1"
                     /translation="MQRNPQLNAKGELQHLLSIEGLPREVLVQILDTAESFVGVTERE
                     VKKVPLLRGKSVFNLFFEPSTRTRTTFEIAAKRLSADVINLNIAVSSQSKGESLLDTV
                     ANLSAMQADIFVVRHNSAGAPYLIARHVKPHEHVVNAGDGRHAHPTQGLLDLFTIRHY
                     KKDFSRLTVAIVGDILHSRVARSLIHGLTTLGAPEVRVIGPQTLMPAGVEALGVRVFH
                     DMRAGLRDCDVVVMLRL"
     gene            complement(696..1202)
                     /gene="pyrR"
                     /locus_tag="VEL04_02830"
     CDS             complement(696..1202)
                     /gene="pyrR"
                     /locus_tag="VEL04_02830"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HYT47332.1"
                     /translation="MTKLPDAERLCAELLKPLKPMVGPKTAMIGIYTGGAWLAERLHA
                     RLGIAAPLGLMDIAFYRDDYHAQGLHHDPKRTKIPFDVNGRELLLVDDVLFTGRTVRA
                     AMNEVFDYGRPASISLVVLADRGGRQLPICAQHCGARVEVPSGMRLRLKRDAAGNLAL
                     ALEPEHAA"
     gene            complement(1186..1578)
                     /gene="ruvX"
                     /locus_tag="VEL04_02835"
     CDS             complement(1186..1578)
                     /gene="ruvX"
                     /locus_tag="VEL04_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HYT47333.1"
                     /translation="MPERAARTVLAFDFGLKRIGVAVGEPELRTAHPLPAVAGFAQIE
                     KLVAEWKPARLVVGLPVREAGEHPLARRVERFARQLHGRFRIPVERVDERFSSVEAES
                     RLRGVRRKAVDSVAAQLILEQFFDDETA"
     gene            complement(1571..2131)
                     /locus_tag="VEL04_02840"
     CDS             complement(1571..2131)
                     /locus_tag="VEL04_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HYT47334.1"
                     /translation="MSSINLTHHFLIAMPNMADPYFSKTLTYICEHNDQGALGLVVNR
                     PIDMTLQALFERLSLTLRQADLAGAPIYFGGPVQTDRGFVLHAPMGNWQSTLRVRDLI
                     GLTTSKDILEAVGRGEGPDKMLVTLGYAGWSPGQLEHELSQNAWLTVEARDGIIFDTP
                     AEERLPAAMELLGLDYARLQDEAGHA"
     gene            complement(2193..2378)
                     /locus_tag="VEL04_02845"
     CDS             complement(2193..2378)
                     /locus_tag="VEL04_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HYT47335.1"
                     /translation="MTENQVQFKTWMCLICGFVYDEAAGLPDEGIAPGTRWEDLPMNW
                     TCPECGARKEDFEMVEI"
     gene            2415..3191
                     /locus_tag="VEL04_02850"
     CDS             2415..3191
                     /locus_tag="VEL04_02850"
                     /inference="COORDINATES: protein motif:HMM:NF020132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HYT47336.1"
                     /translation="MPSSPPLVLTFAASDPTGGAGLQADVLTLASLGCHPLSVVTALT
                     VQDTSGVEQLEAVDAELVRRQASRLLAESRVAAFKLGVLGSEANVRAIARVVAEHARV
                     PLVLDPVLASGRGDVLASEAMIEALLELIVPRATLATPNSLEARRLGGEKRLLERGCR
                     YVLVTGTHEPGAEVVNRLFGPQGLVREDRWPRLPGSYHGSGCTLASAIAAALASGLDV
                     PAAVRDAQEYTWQALSSGFRPGKGQYIPDRFFRARETESR"
BASE COUNT          535 a         1096 c         1106 g          544 t
ORIGIN      
        1 gcagccgcag catcaccacc acatcgcaat ccctgaggcc ggcgcgcatg tcgtggaaca
       61 cccgcacgcc aagcgcctcg actccggcgg gcatcaaggt ctgcggcccg atcacacgca
      121 cttcgggcgc gccgagcgtg gtgagcccgt ggatcagcga gcgggcgacg cgcgaatgca
      181 ggatgtcgcc gacgatcgcc accgtcaggc ggctgaaatc cttcttgtaa tgccggatgg
      241 tgaacaggtc gagcagcccc tgcgtcgggt gcgcatgccg gccgtcgccc gcgttcacca
      301 cgtgctcgtg cggcttgaca tgccgcgcga tgagatacgg cgcccccgcc gaattgtggc
      361 gaacgacgaa aatgtcggct tgcatcgccg acagattcgc caccgtgtcg agcagcgact
      421 cgcccttcga ctgcgaggac acggcgatgt tgagattgat gacgtcggcc gagagccgct
      481 tggcggcgat ctcgaaggtg gtgcgggtgc gcgtcgatgg ctcgaagaac agattgaaca
      541 cgctcttgcc gcgcaacagc ggcacctttt tcacctcgcg ctcggtgacg ccgacgaacg
      601 actcggcggt gtcgaggatt tgcaccagca cctcgcgcgg cagaccttcg atggacagca
      661 gatgctgcag ctcccctttc gcattgagct gggggttacg ctgcatgttc cggctcgagg
      721 gcgagggcaa ggtttcccgc agcgtcgcgt ttcagccgca ggcgcatccc cgagggcacc
      781 tcgacgcgcg cgccgcaatg ctgcgcgcag atcggcagct gccgcccgcc gcggtcggcg
      841 agcacgacga gcgagatgct cgccgggcgg ccatagtcga acacctcgtt catcgccgcg
      901 cgcaccgtgc gaccggtgaa cagcacgtcg tcgaccagca gcagctcgcg gccgttgacg
      961 tcgaacggga tcttggtgcg cttcgggtcg tgatgcaggc cttgcgcgtg atagtcgtcg
     1021 cggtagaacg cgatgtccat cagccccagg ggcgccgcaa tgccaagcct ggcatgcagc
     1081 cgctccgcga gccacgcgcc gcccgtgtag atgccgatca tagccgtctt ggggccgacc
     1141 atcggcttca acggcttcaa caattccgcg cagaggcgct cggcgtcagg cagtttcgtc
     1201 atcgaaaaac tgctcgagga tcagttgcgc cgcgaccgag tcgacggctt ttcgcctgac
     1261 tccgcgcagc cggctctcgg cctcgaccga gctgaagcgc tcgtccacgc gctcgaccgg
     1321 aatgcggaat ctgccgtgga gctgccgcgc gaagcgctcg acgcgccgcg cgagcgggtg
     1381 ctcgcccgcc tcgcgcaccg gcagcccgac gacaaggcgt gccggcttcc actccgcgac
     1441 cagcttctcg atctgcgcga agcccgcaac cgccggcaag ggatgcgccg tccgcaattc
     1501 gggctcgccg accgcgacac caatgcgctt taaaccgaaa tcgaaggcga ggaccgtgcg
     1561 cgcggcgcgc tcaggcatgt cccgcttcgt cctgcagcct cgcgtagtcg aggccgagga
     1621 gctccatcgc cgccggcagc cgctcctcgg cgggcgtgtc gaagatgatg ccgtcgcgcg
     1681 cctcgacggt gagccaggca ttttgcgaca gctcgtgctc gagctgccca ggcgaccatc
     1741 ctgcgtagcc gagggtgacg agcatcttgt ccgggccttc gccccgccca accgcttcaa
     1801 ggatgtcctt cgaggtggtg agcccgatca ggtcgcgcac gcgaagcgtc gactgccagt
     1861 tgcccatcgg cgcgtgcagc acgaagccgc ggtcggtctg caccggcccg ccgaagtaga
     1921 tcggcgcgcc cgcgaggtcg gcctggcgca gcgtgagcga cagtcgctcg aacaaggcct
     1981 gcagcgtcat gtcgatcggg cgattcacca ccaggccgag cgcgccctga tcgttgtgct
     2041 cgcagatata ggtgagggtc ttcgagaagt acggatcggc catgttgggc atggcgatca
     2101 ggaaatgatg cgtcaggttg atcgaggaca tgctcccacc ccgtatttta ccgcagcagg
     2161 gagctttgag cttttcatgg cgcgaaagtt ttctagattt ccaccatctc gaaatcttcc
     2221 ttgcgggcgc cgcattcggg acaggtccag ttcatcggca ggtcttccca gcgcgtgcct
     2281 ggcgcgatgc cctcgtcggg cagcccggca gcttcgtcgt acacgaagcc gcagatcagg
     2341 cacatccagg tcttgaattg aacttgattt tcggtcattg gccgcggcag tagaatgggc
     2401 ccggatatta gcaaatgccg tcatcgccgc cgctcgtgct caccttcgcc gcatcggatc
     2461 cgaccggcgg cgcgggattg caggccgacg tcctgacgct cgcgagcctg ggatgtcatc
     2521 cgctctcggt cgtcaccgcg ctcaccgtgc aggacaccag cggcgtcgag cagctcgaag
     2581 ccgtcgacgc ggagctcgtc aggcgacagg cgagccggct gctcgcggaa tcgcgcgtcg
     2641 cggcgttcaa gctcggggtg ctcggctcgg aagcgaacgt gcgcgcgatc gctcgggtcg
     2701 tcgccgagca tgcgcgcgtg ccgctggtgc ttgacccggt gctcgcatcg ggtcgcggcg
     2761 acgtgctcgc gagcgaagcg atgatcgaag cgctgctcga gctgatcgtg ccgcgcgcca
     2821 cgctcgccac gccgaacagc ctcgaagcgc gccgcctcgg cggtgagaag cggctgctcg
     2881 agcgcggctg ccgctacgtg ctcgtcaccg gaacgcacga accgggcgcg gaggtggtca
     2941 accggctgtt tggcccgcaa gggctggtgc gggaggaccg ctggccgcgg ctgcccggca
     3001 gctatcacgg ctcgggctgc acgctcgctt cggcgattgc ggcggcgctg gcgagcggcc
     3061 tcgacgtgcc ggcggcggtg cgcgatgcgc aggaatatac ctggcaggcg ctctcgagcg
     3121 ggttccgccc cggcaagggg cagtacattc ccgatcgctt tttccgggcg cgcgagaccg
     3181 agagcaggtg aagctgcgcg ggttgtacgc gatcaccccg gaaggccctg gactgactca
     3241 gaaggttggc gacgcgctta aaggaggcat tgccctgttg c
//
LOCUS       DASPXW010000120         4009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1800463, whole genome shotgun sequence.
ACCESSION   DASPXW010000120 DASPXW010000000
VERSION     DASPXW010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1800463"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VEL04_02855"
     CDS             complement(<1..313)
                     /locus_tag="VEL04_02855"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HYT47337.1"
                     /translation="MKSPPRAAGCVVFRRTPQGVRYLVLRAFKNWDFPKGLVEAGEEQ
                     LECAKREVEEETGLTALDFPFGDEFRETLPYAGRKVARYYLAETEQVEIELPVSPELG
                     RP"
     gene            complement(430..624)
                     /locus_tag="VEL04_02860"
     CDS             complement(430..624)
                     /locus_tag="VEL04_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47338.1"
                     /translation="MLKTAFWQNAARSLHPSIRQRYIRYFELAERWELALDAALEVWS
                     RAKAGLARLFQTPPRAKHEH"
     gene            complement(617..790)
                     /locus_tag="VEL04_02865"
     CDS             complement(617..790)
                     /locus_tag="VEL04_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47339.1"
                     /translation="MTKLRYPTPDELYALEIAARRARSKEMARLVRAGARALRAGIAR
                     IAAVLSARKVGHA"
     gene            888..1835
                     /gene="gcvA"
                     /locus_tag="VEL04_02870"
     CDS             888..1835
                     /gene="gcvA"
                     /locus_tag="VEL04_02870"
                     /inference="COORDINATES: protein motif:HMM:NF008352.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HYT47340.1"
                     /translation="MIYRLPPLNALRVFEAAARHLSFKEAASELNITQAAVSHQVKSL
                     EEYLGVELFRRSGRGVQLTEAARACLPRLREGFDALAAAVELIRERGAETDLMITAPP
                     VFTARWLMPRLADFSKRHPKIEVRVVGSSKMVDAGALDSAALVGNLDLRDDTSGVEIH
                     LGLGEYPGYRADRLFGVSMVVVASPELVKGEAALRQPADLARHVLLHDDAMDLVAHGN
                     AWQKWLEAAGVADRVDAAHGPHFSSNILSLEAASQKLGVALALRPLVDADIASGRLVA
                     PFAVGVKPLGAYYLVCPEVIAERPAVAAFRKWLLEKAGT"
     gene            1863..2129
                     /gene="infA"
                     /locus_tag="VEL04_02875"
     CDS             1863..2129
                     /gene="infA"
                     /locus_tag="VEL04_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HYT47341.1"
                     /translation="MAKEELIEMDGTVQEVLPNTQFRVTLSNGASVLAYAGGKMRQRH
                     IRIIAGDRVTLEISPYDVTRGRINFRHLDVRPAPQRRKMPYRKK"
     gene            2221..2631
                     /locus_tag="VEL04_02880"
     CDS             2221..2631
                     /locus_tag="VEL04_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HYT47342.1"
                     /translation="MTSRDFSRWMWSEALSMLDRAERLHRQFFTHAAVAWEPPVDIIE
                     AGDALQVQVALPGVAADAIAVSLDGAGVTISALRAFPCRDDTSQVHRIEIPYGRFERH
                     IGLPLGGPDAPLELAGKSLADGVLTLTFRKKEGT"
     gene            2628..>4009
                     /locus_tag="VEL04_02885"
     CDS             2628..>4009
                     /locus_tag="VEL04_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HYT47343.1"
                     /translation="MNSKVLADGKNGVSAAAADKARAGAAKPLPDDALIVIPMRNTVL
                     FPGVISPITVGRATSVAAAQEAVRTEKRIGFLLQRDPQKNDVKPEDLFWVGTAGLVVR
                     YITGNEGAHHLVVQGQSRFRALEFLEGWPFLVARVSMVEAKDAMTPELEARFLQLKEQ
                     ATEAIQLLPNAPEELAVVVQGVSSPGQLADMVANLLDVKNEDKQALLETFDLRRRLDK
                     VLELLGERVGVLRISREIGEKTKKQFDERQREHVLREQMRQIQKELGEDEDGETGIAE
                     LKKAIEDAGMPEETHKYALKELKRLQRMGEGSAENSMLRTYLEWLSELPWKTEEGKPI
                     DIAAARKVLDEDHYGLDKIKRRILEHLAVRKLNPAGKSPILCFVGPPGVGKTSLGQSI
                     ARATGRKFQRIALGGVHDEAEIRGHRRTYIGALPGSVIQALRRAESRNTVLMFDEIDK
                     LGMGGFHGDPS"
BASE COUNT          677 a         1333 c         1384 g          615 t
ORIGIN      
        1 cggggcggcc aagctcgggc gacaccggaa gctcgatctc gacctgctcg gtttccgcga
       61 ggtaatagcg cgcgaccttg cggccggcgt agggcagggt ttccctgaat tcgtcgccga
      121 aggggaagtc gagggcggtg agcccggttt cctcctcgac ctcgcgcttg gcgcactcga
      181 gctgctcctc gcccgcttcg accaggccct taggaaagtc ccagttcttg aacgcgcgca
      241 gcacgaggta gcgcacgccc tgcggggtgc ggcgaaacac cacgcagccc gcggcgcggg
      301 gaggggattt cacaccccca atgataggaa tgtttcggca ataaaagaat gctcgccgcc
      361 cgggcgcgcc ctggccagag gaggaggagg acggcttcgg attcgcccgg acggcgaggc
      421 aagcgtggat cagtgctcgt gcttcgcgcg cggcggcgtt tgaaacagcc ttgcaagccc
      481 ggccttcgcg cgcgaccaga cttcgagggc ggcgtcgagc gcgagttccc agcgttccgc
      541 aagttcgaag tagcggatgt agcgctggcg gatcgacggg tgcagcgagc gcgcggcgtt
      601 ttgccagaat gcggtcttaa gcatggccaa ccttccttgc gctgagcacc gccgcaatgc
      661 gtgcgatgcc cgcccgcagt gcgcgcgcac cggcgcggac caggcgcgcc atttccttgc
      721 tgcgcgcgcg ccgcgccgcg atctcgagtg cgtacaattc gtccggggtc ggataacgca
      781 gtttcgtcat ggtggttctc cttttccttt tccttcgaca tgcaacatgg ggccttgtca
      841 ctgctgaaac aaacgatgat ttcttacgtc ttgcataaga aaaactcatg atctatcgcc
      901 tgccgcccct gaacgcgctg cgggtattcg aggccgccgc gcgccacctc tcgttcaagg
      961 aggcggcgag cgagctcaac atcacgcagg ccgcggtgag ccatcaggtg aagagcctcg
     1021 aggaatacct cggcgtggag ctgttccgcc gctcgggccg cggcgtgcag ctcaccgagg
     1081 cggcgcgcgc ctgcctgccg cggctgcgcg agggcttcga cgcgctcgcc gccgcggtcg
     1141 agctgatccg cgagcgcggc gccgagaccg atctgatgat caccgcgccg ccggtgttca
     1201 ccgcgcgctg gctgatgccg cggctcgccg acttctcgaa gcgccatccg aagatcgagg
     1261 tgcgcgtggt cggctcgagc aagatggtcg acgccggcgc gctcgattcc gccgcgctgg
     1321 tgggaaacct ggatctgcgg gacgacacct cgggcgtcga gatccacctg ggactgggcg
     1381 aatatcccgg ctaccgggcc gaccgcctgt tcggcgtctc gatggtggtg gtggcgagcc
     1441 ccgaactggt gaagggcgaa gcggcgctca ggcagcccgc ggacctcgcg cgccacgtgc
     1501 tgctgcacga cgacgcgatg gacctggtcg cccacggcaa cgcctggcag aagtggctcg
     1561 aggcggccgg agtcgccgac cgcgtggatg cggcgcacgg gccgcatttc tcctcgaaca
     1621 tcctgtcgct cgaggcggcg tcgcagaagc tcggcgtggc gctcgcgctt cgccctctcg
     1681 tcgacgcgga tatcgcgtcg ggccgactgg tggcgccgtt cgcggtcggg gtgaagccgc
     1741 tcggcgcgta ttacctggtg tgtcccgagg tgatcgccga gcggcccgca gtggcggcat
     1801 tcagaaaatg gctgctggaa aaagccggaa cctaggcgcc atgcgatata ctgcgcgacc
     1861 ccatggcgaa agaagagctc atcgagatgg acggcaccgt gcaggaggtg ctgccgaaca
     1921 cgcagttccg cgtcacgctg tcgaacggcg cgagcgtgct cgcgtatgcc ggcggcaaga
     1981 tgcgccagcg ccatatccgc atcatcgcgg gcgaccgcgt caccctcgag atctcgccct
     2041 acgacgtcac ccgcgggcgc atcaatttcc gccacctcga cgtccgcccg gcgccccagc
     2101 gccggaaaat gccctaccgc aagaagtaat tcctctttgg tttcaggtag ctcggtgtag
     2161 gctcggcgcg ttcactgctg cgcccttgat tcgtgcggcg gctgccgcca cattacgcca
     2221 atgacctccc gggacttctc gcgctggatg tggagcgagg cgctgtcgat gctcgaccgc
     2281 gccgagcgcc tgcaccggca gttcttcacc catgccgcgg tggcctggga gccgccggtc
     2341 gacatcatcg aggccggcga cgcgctgcaa gtgcaggtcg cgctgcccgg cgtggcggcc
     2401 gacgccatcg ccgtcagcct ggacggcgcg ggcgtcacca tctccgcgct gcgcgcgttt
     2461 ccctgccgcg acgacacctc gcaggtgcac cgcatcgaga tcccctacgg caggttcgag
     2521 cgccacatcg gcctgccgct cggcggtccc gacgcgccgc tcgagctcgc cggcaagtcg
     2581 ctcgccgatg gcgtcctcac cctgaccttc aggaagaagg aaggcacatg aacagcaaag
     2641 ttcttgcaga cgggaagaat ggcgtttcgg ccgccgccgc cgacaaggcc agggcgggcg
     2701 ccgcaaagcc gctgcccgat gacgcgctga tcgtcatccc gatgcgcaac accgtgctgt
     2761 tccctggcgt gatctcgccc atcacggtcg ggcgcgcgac gtcggtcgcg gcggcgcagg
     2821 aggcggtgcg caccgagaag cgcatcggct tcctgttgca gcgcgatccg cagaaaaacg
     2881 acgtcaagcc ggaagacctg ttctgggtcg gcacggcggg cctggtggtg cgctacatca
     2941 ccggcaacga gggcgcgcac cacctcgtgg tgcaggggca gagccgcttc cgcgcgctcg
     3001 agttcctcga gggctggccg ttcctggtgg cgcgcgtctc gatggtcgag gcgaaggacg
     3061 cgatgacgcc cgagctcgag gcgcgcttcc tgcagctcaa ggagcaggcg accgaggcga
     3121 tccagctgct gcccaacgcg cccgaggagc tcgcggtggt ggtacagggc gtgagctcgc
     3181 cggggcagct cgccgacatg gtcgcgaacc tgctcgacgt gaagaacgag gacaagcagg
     3241 ccctgctcga gaccttcgac ctcaggcgcc ggctcgacaa ggtgctcgag ctgctcggcg
     3301 agcgcgtcgg cgtgctgcgc atctccaggg agatcggcga gaagaccaag aagcagttcg
     3361 acgagcgcca gcgcgagcac gtgctgcgcg agcagatgcg ccagatccag aaggagctcg
     3421 gcgaggacga ggacggcgag accgggatcg ccgagctgaa gaaggcgatc gaggacgccg
     3481 gcatgcccga ggagacgcac aagtacgccc tcaaggagct caagcgcctg cagcgcatgg
     3541 gcgagggcag cgccgagaac tcgatgctgc ggacttacct ggaatggctt tcagagctgc
     3601 cgtggaaaac ggaagagggc aaaccgatcg atatcgccgc agcgcgaaaa gtcctcgacg
     3661 aggaccacta cggcctcgac aagatcaagc gccgcattct cgagcacctc gcggtgagga
     3721 agctgaatcc ggccggcaag agcccgatcc tgtgcttcgt cgggccgccg ggggtcggca
     3781 agacctcgct cggccagtcg atcgcgcgcg ccacggggcg caagttccag cgcatcgcgc
     3841 tcggcggtgt gcacgacgag gccgagatcc gcggccaccg gcgcacctac atcggcgcgc
     3901 tgccgggcag cgtgatccag gcgctgcgcc gcgccgagtc gaggaacacg gtgctgatgt
     3961 tcgacgagat cgacaagctc ggcatgggcg gcttccacgg cgacccgtc
//
LOCUS       DASPXW010000121         2707 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1423061, whole genome shotgun sequence.
ACCESSION   DASPXW010000121 DASPXW010000000
VERSION     DASPXW010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1423061"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /gene="mltG"
                     /locus_tag="VEL04_02890"
     CDS             complement(<1..838)
                     /gene="mltG"
                     /locus_tag="VEL04_02890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HYT47344.1"
                     /translation="MRILQRPHLKTLFKTFRTLLSFAVLTAGGYGVWYLMTPLGVRVF
                     PVEVEIPRGAGFRAAAAVLEKAGVELRHREFELLARALGRERDIKAGNYQLTERPRPL
                     ELLEKLTRGDVTQAEVRLIEGWTFGQFRAALDASAELRHDTTGVDETQILALLQAAEP
                     HPEGLFFPDTYLFARGSSDLAVLRRAYRAMQRHLQTEWEARDPGAPYRSPYEALIMAS
                     IIEKETGRAEERDQIGGVLANRLRIGMRLQADPTVIYGLGDAFNGNLKKSHLLDDGPY
                     NTY"
     gene            845..1591
                     /locus_tag="VEL04_02895"
     CDS             845..1591
                     /locus_tag="VEL04_02895"
                     /inference="COORDINATES: protein motif:HMM:TIGR03317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HYT47345.1"
                     /translation="MERARLTRYGLLSVSGDDAREFLHAQLTNDIRALPADRAALAGW
                     CSAKGRLLASFLVIPAPEGFLLQVARDLAAGVAKRLAMFVLRSKVKIADQSESWIQHG
                     VWDADLTQPEVSWQGRIAAVRVGERRYLQLGPAADMTAPPTTGEENWTLREIRAGRPL
                     ITSATQDQFVPQMVNLEKLGGIDFQKGCYPGQEIVARAQYRGQVKRRMVHAHAPAGTQ
                     LAPGQDYNGGTVVDAAPAQHGTELLAVMPL"
     gene            1588..1854
                     /locus_tag="VEL04_02900"
     CDS             1588..1854
                     /locus_tag="VEL04_02900"
                     /inference="COORDINATES: protein motif:HMM:NF027614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4936 family protein"
                     /protein_id="HYT47346.1"
                     /translation="MNYYVYYKIEPQRLEELRRAVEGLFDVVERKFGVRGRWMRRRDD
                     PATYMEVYEDVEGGKAFEALLEREGAKLGVPRKLECFISAGKPA"
     gene            complement(1829..2101)
                     /locus_tag="VEL04_02905"
     CDS             complement(1829..2101)
                     /locus_tag="VEL04_02905"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HYT47347.1"
                     /translation="MICFDRPDSAALRDAHRAAHVEFLNANAARIVFGGPLKSAADGP
                     STGALIVVDCTTRQEADAFIGADPFYRGGVYESVSVRAFKRVFPQK"
     gene            2129..>2707
                     /gene="lsrF"
                     /locus_tag="VEL04_02910"
     CDS             2129..>2707
                     /gene="lsrF"
                     /locus_tag="VEL04_02910"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: protein motif:HMM:NF006081.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HYT47348.1"
                     /translation="MADKDDIKEGKDFGIGIPQENRPFYVKGGGALDWGMQNRLARIF
                     NPASGRTVMLAFDHGYFQGPTTGLERIDLSIVPLIPHADVLMCTRGALRSVIPPSATK
                     AVVLRCSGGQSILTELSREAIAVDIEDAIRVNAAAITTQIYIGAQYEHQSIRNLVQLI
                     DTGNRYGIPTMAVTGVGADMKRDERYFGLATRI"
BASE COUNT          459 a          866 c          924 g          458 t
ORIGIN      
        1 cataggtgtt gtacgggccg tcatcgagca ggtggctttt cttcaggttg ccgttgaagg
       61 catcgccgag gccgtaaatc accgtcgggt cggcctgcag ccgcatgccg atgcgcaggc
      121 ggttggccag cacgccgccg atctggtcgc gctcctccgc gcggccagtc tccttttcga
      181 tgatcgacgc catgatgagc gcttcgtacg gcgagcggta cggggcgcct ggatcgcgcg
      241 cttcccattc cgtttggaga tggcgctgca ttgcgcggta ggcacgccgc agcaccgcga
      301 ggtcgctcga gcccctggcg aaaagatacg tgtcggggaa gaacaggcct tccggatggg
      361 gctctgccgc ctgcaggagc gcgagaatct gcgtctcgtc cacgcccgtg gtgtcgtgcc
      421 gcagctcggc gctcgcgtcg agcgcggcgc ggaactggcc gaaagtccag ccttcgatca
      481 gcctcacctc ggcctgggtg acatcgcctc gcgtcagctt ttcgagcagc tcgagcggcc
      541 tcgggcgctc cgtgagctgg tagtttccgg ccttgatgtc gcgctcgcgc ccgagcgcgc
      601 gcgcgagcag ctcgaactcg cggtgtctca gctcgacgcc cgctttttcg agcaccgcgg
      661 cggcagcgcg gaagccggca ccgcgcggaa tctcgacttc gaccggaaat acgcgcacgc
      721 cgagcggcgt catcagatac cagacgccgt agccgcccgc ggtgagcacc gcgaaagaca
      781 gcagcgtgcg aaaggttttg aagagggttt tcaggtgagg tcgctgtaaa attctcattc
      841 tacgatggag cgcgcgcgcc tcacccgcta tggattgctg tcggtttcgg gcgacgacgc
      901 gcgtgaattc cttcacgccc agctgaccaa cgacatccgc gcgctgccgg ccgatcgcgc
      961 cgccctcgcc ggctggtgct cggcgaaagg gcgcctgctc gcgagcttcc tggtcattcc
     1021 ggcaccggag ggcttcctgc tccaggtggc gcgcgatctc gccgccggcg tggcgaagcg
     1081 gctggcgatg ttcgtgctgc gctcgaaggt gaagattgcc gaccagagcg agtcctggat
     1141 ccagcacggg gtgtgggacg ccgacttgac gcagcccgag gtcagctggc agggccgtat
     1201 cgccgcggtg cgggtgggcg agcgccgtta cctgcaactc gggccggccg ccgacatgac
     1261 tgctccgccc acgaccggcg aagagaactg gactctgcgg gaaatccgcg ccggccggcc
     1321 gttgatcacc agcgcgacgc aggaccagtt cgtgccgcag atggtcaacc tggagaagct
     1381 cggcggcatt gatttccaga agggctgcta cccggggcag gaaatcgtcg cgcgcgcgca
     1441 gtatcgcggc caggtcaagc gccgcatggt gcatgcgcac gcgcccgccg gcacgcagct
     1501 cgcgcccggg caggactaca acggcggcac ggtggtcgac gcggcaccgg cgcagcacgg
     1561 caccgagctg ctcgcggtga tgccgctttg aattattacg tctactacaa gatcgagccg
     1621 cagcggctgg aagagctgcg gcgcgcagtc gagggcctgt ttgatgtcgt cgagaggaaa
     1681 ttcggcgtgc gcggccgctg gatgcgccgg cgcgacgacc cggcgaccta catggaggtt
     1741 tacgaggacg tcgagggcgg caaagcgttc gaggcattgc tcgagcgcga gggcgcgaag
     1801 ctcggcgtgc cgcgcaagct cgagtgcttc atttctgcgg gaaaacccgc ttgaaggcgc
     1861 gcaccgagac gctttcgtag accccgcccc ggtaaaacgg gtctgcgccg atgaatgcgt
     1921 cggcttcctg gcgtgtcgtg caatcgacga cgatcagcgc ccccgtgctc ggaccgtcag
     1981 ccgcgctttt caggggaccg ccgaagacga tgcgagccgc gttggcgttg aggaattcta
     2041 cgtgcgcggc tcggtgcgcg tcgcgtagcg cggccgagtc gggacggtcg aaacagatca
     2101 cggcgtacat gatatggtag gaggaattat ggctgacaaa gacgatatca aggaaggcaa
     2161 ggacttcggc atcggcattc cccaggagaa ccgccccttc tacgtcaagg gcggcggcgc
     2221 gcttgactgg gggatgcaga accgtctcgc acgcattttc aatccagctt ccggccggac
     2281 cgtgatgctt gcgttcgacc acggctactt ccaggggccg acgaccgggc tcgagcgcat
     2341 cgatctcagc atcgtgccgt tgatcccgca tgccgatgtg ctgatgtgca cgcgaggcgc
     2401 cctgcgttcg gtgatcccgc cctcggcgac caaagccgtg gtgctgcgct gctcgggcgg
     2461 acagagcatc ctcaccgagc tttcgcgaga ggccatcgcc gtcgacatcg aggacgcaat
     2521 ccgggtcaat gccgcggcga ttaccacgca gatctacatc ggcgctcagt acgagcacca
     2581 gtcgatcagg aacttggtcc agctgatcga caccggcaac cgctacggca tcccgaccat
     2641 ggcagtcacg ggcgtgggtg ccgacatgaa gcgggacgag cgctatttcg gcctggcgac
     2701 gcgcatc
//
LOCUS       DASPXW010000122         2198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1674453, whole genome shotgun sequence.
ACCESSION   DASPXW010000122 DASPXW010000000
VERSION     DASPXW010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1674453"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VEL04_02915"
     CDS             complement(<1..477)
                     /locus_tag="VEL04_02915"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HYT47349.1"
                     /translation="MVTVVQLRQSSSAAALGPIAEGLAGAEHALIERALQFAEPLYGA
                     QRLSTGEPTWAHALGIAANLAAIDLDAPGRAAGILFAAPKHLSDFEDLRKQFGEEIAS
                     LAAGVEKLYQLRVATRANPQEQSEVLRKMVLGMVEDVRVVLIRLASRTQTLRFFAKN"
     gene            494..1261
                     /locus_tag="VEL04_02920"
     CDS             494..1261
                     /locus_tag="VEL04_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006409107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HYT47350.1"
                     /translation="MGWLTDWRRRRVLARHRIDDALWADATRGLEFIKSLSASETGKL
                     RRLVVLFLAEKEFAAAHGLEITDAMRLSIAAQACLPILELGLDWYAGWSGVVVYPGDF
                     RVRRTEVDEHGVVHEWEDELAGEAMPGGPVVLSWDAAAHDPAMNVVIHEFAHKLDMLS
                     GAADGLPPLHAGMERRAWHAAFEEAYQGFCDAVERGKDTWLDPYAAEHPSEFFAVMSE
                     AFFEEPGETQRRYPTVYEQLKLFYRQDPALRQAAAEK"
     gene            complement(1235..1960)
                     /locus_tag="VEL04_02925"
     CDS             complement(1235..1960)
                     /locus_tag="VEL04_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT47351.1"
                     /translation="MEAEILALEEVVGGYGKLTVLNGTSFKVARGAITTLIGPNGAGK
                     STVFKTVFGLLPVERGRIVFEGREITNLDPQRLLKLGISYVPQGRNVFPELSVRHNLE
                     LGATAAGEGTPIAARIESLMDRFPMLRRKAGAQASTLSGGEQKLLEIARGLLLEPKLM
                     LIDEPSIGLSPRLVQDIFGILQDLRAKGVTILMVEQNAKRALEISDFGVVLELGHTRV
                     EGAAHAILADPRVGQLFLGGGLA"
     gene            complement(1950..>2198)
                     /locus_tag="VEL04_02930"
     CDS             complement(1950..>2198)
                     /locus_tag="VEL04_02930"
                     /inference="COORDINATES: protein motif:HMM:NF023816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT47352.1"
                     /translation="RLVLIDEPAGGVNPTMLAGLRERLQAINQTEGATFVVIEHNMEF
                     VMSLCTRILVLAEGRLIAEGAPEAIRKDRGVIEAYLGS"
BASE COUNT          354 a          732 c          754 g          358 t
ORIGIN      
        1 attcttggcg aaaaaccgca aggtctgcgt cctgctcgca aggcggatca gcaccacgcg
       61 tacgtcctcc accatgccga ggaccatctt gcgcagcacc tcgctctgct cctgcggatt
      121 ggcgcgcgtc gcgacgcgca gctggtagag cttctccacg cccgcggcga ggctggcgat
      181 ctcttcgccg aattgctttc tcaagtcctc aaagtcagaa agatgcttcg gtgcggcaaa
      241 caggatcccg gcggcgcgcc cgggcgcatc gaggtcgatc gccgcgaggt tcgccgcaat
      301 gccgagcgcg tgcgcccagg tcggctcgcc ggtcgaaagc cgttgcgcgc catatagcgg
      361 ctcggcgaat tgcagagcgc gctcgatcag ggcatgctcc gccccggcca gcccttccgc
      421 gatcgggccg agcgccgcgg cgctgctact ctggcgaagc tggacgacag tgaccatgcg
      481 gtattttacg gggatgggct ggctgaccga ctggcgccgc aggcgggtgc tcgcaagaca
      541 ccgcatcgat gatgcgcttt gggctgacgc cacgcggggc ctggaattca tcaaaagcct
      601 gtctgcctcg gaaaccggga agttgaggcg tctggtcgtc ctgttcctcg ccgagaaaga
      661 gttcgcagcg gcacacggcc tggagatcac cgatgccatg cggctgtcga tcgccgcgca
      721 ggcctgcctg ccgattctcg agctcggcct ggactggtac gcgggctgga gcggcgtggt
      781 ggtgtacccc ggcgactttc gcgtgcgccg cacggaggtg gacgagcacg gcgtggtgca
      841 cgaatgggaa gacgagctcg cgggcgaggc gatgcccggc gggccggtgg tgctgtcctg
      901 ggacgccgcg gcgcatgatc cggcgatgaa cgtcgtgatc cacgagttcg cgcacaagct
      961 cgacatgctg agcggcgcgg ccgacggcct gccgccgctg catgcgggga tggagcggcg
     1021 cgcgtggcac gcggcgttcg aggaggccta ccagggcttt tgcgacgccg tggagcgcgg
     1081 caaggacacc tggctcgacc cctacgccgc cgagcaccca tccgagttct tcgctgtgat
     1141 gagcgaggcg ttcttcgagg aaccggggga gacgcagcgc cgctacccga cggtgtacga
     1201 gcagctcaag ctcttctacc gccaggaccc ggcattacgc caggccgccg ccgagaaata
     1261 gctgtcccac gcgcggatcg gcgaggatcg catgtgccgc gccctcgacg cgcgtgtggc
     1321 cgagctccag cacgacgccg aagtccgaga tctcgagcgc gcgcttggcg ttctgctcga
     1381 ccatcaggat ggtgacgccc ttggcgcgca gatcctgcag gatgccgaag atgtcctgca
     1441 ccagccgcgg cgacaggccg atcgacggct cgtcgataag catgagcttc ggctccagca
     1501 gcaggccgcg cgcgatctcg agcagcttct gctcgccgcc ggacagcgtc gaggcctgcg
     1561 cgccggcctt gcgtcgaagc atggggaagc ggtccatgag cgattcaatg cgcgcggcga
     1621 tgggcgtccc ctcgcccgcg gcggttgcgc cgagctcgag gttgtgccgc acgctcagct
     1681 ccgggaacac attgcggccc tgcggcacgt agctgatccc cagcttgagc agccgctgcg
     1741 gatcgagatt ggtgatctcc cgaccctcga acacgatgcg gccgcgctcg accggcagca
     1801 ggccgaacac ggtcttgaac acggtggatt tgccggcgcc gttcggcccg atgagcgtgg
     1861 tgatggcgcc gcgcgccacc ttgaagctcg tgccgttgag cacggtcagc ttgccgtagc
     1921 cgccgaccac ctcctctagc gcgagaatct cagcttccaa gataagcctc gatcaccccg
     1981 cgatccttgc gaatggcctc gggcgcgccc tcggcgatga ggcggccttc ggcgagcacc
     2041 aggatgcggg tgcatagcga catcacgaac tccatgttgt gctcgatgac cacgaaggtc
     2101 gcgccttcgg tctggttgat cgcctgcagg cgctcgcgca gacccgcgag catcgtcggg
     2161 ttgaccccgc cggcgggctc gtcgatgagc accaggcg
//
LOCUS       DASPXW010000123         2469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_163101, whole genome shotgun sequence.
ACCESSION   DASPXW010000123 DASPXW010000000
VERSION     DASPXW010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163101"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /gene="hflK"
                     /locus_tag="VEL04_02935"
     CDS             <1..954
                     /gene="hflK"
                     /locus_tag="VEL04_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HYT47353.1"
                     /translation="YIVVEGQRGVVLTFGRFSQETGPGLRWRVPWPIQSNEIVNLFQV
                     RTLEVGYRNNVRTKVLKESLMLTDDENIIDLQFAVQYVVKDSRDFIFKVRRPDESAMQ
                     IAETAMREVIGKNRMDAILYETQVDVANKARDLMQAIHDRYGTGIQISTVTIQNAQPP
                     EQVQAAFDDAVKAGQDRERQKNEGQAYANDVIPRARGTASRLLQEAEGYKQRVIASAE
                     GDASRFKQVLAEYAKAPAVTRERIYIDTMQQILSSTNKIMMDYRGPGSLLYLPLDKIM
                     QQSGAGPLTESQAAQRPSEPTPPEPGPRSRETLRNRDRGER"
     gene            977..1864
                     /gene="hflC"
                     /locus_tag="VEL04_02940"
     CDS             977..1864
                     /gene="hflC"
                     /locus_tag="VEL04_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HYT47354.1"
                     /translation="MRALGPVVAILIVVALLASGTLFTVDQRQNAIVFQLGEVTDVVQ
                     RPGLHFKWPLLQNVRLFDMRILTFDDAEPLRFLTQGNRPVLVDSFVKWRIADVKQYYV
                     SVQGDEFRAMTRIKQTVAGVLRDEFGVRTVHEVVSGEREQIMARVRDKVDQDLKRIGV
                     EIIDVRLKRVDLPPDVSESVYRRMEAERKRIANELRSTGAAEAEKIRADADRQREVIL
                     AEAFRDSQRTRGEGDAKAANIYAGAFSQNPEFFSFYRSMEAYRSTFRGRNDLMLLETN
                     SDFLRYFRDSLGRAPATRK"
     gene            1868..2056
                     /locus_tag="VEL04_02945"
     CDS             1868..2056
                     /locus_tag="VEL04_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008349392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HYT47355.1"
                     /translation="MGTLLLMALALMLILEGVLPFLAPNLWRDTFRRITQMSDGQIRF
                     VGLSSMLVGLLLLLWARS"
     gene            2053..>2469
                     /locus_tag="VEL04_02950"
     CDS             2053..>2469
                     /locus_tag="VEL04_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HYT47356.1"
                     /translation="MTVKWLLPEFIEDILPAEAARIEALRRTILDLLFKNKFQLVIPP
                     LLEYIDSLLTGSGHDLELRTFKVVDQLSGRMMGLRADITPQVARIDAHLLNRRGVTRL
                     CYCGAVLHTRPASPGATREPMQIGAEIFGHAGLEADL"
BASE COUNT          432 a          833 c          823 g          381 t
ORIGIN      
        1 tacatcgtgg tcgagggcca gcgcggcgtg gtgctcacct tcggccgctt ctcgcaggag
       61 accgggccgg gcctgcgctg gcgggtaccc tggccgatcc agtcgaacga gatcgtcaac
      121 ctgtttcagg tgcgcaccct cgaagtcggc taccgcaaca acgtgcgcac caaggtgctg
      181 aaagagtcgc tgatgctgac cgacgacgag aacatcatcg acctgcagtt cgcggtgcag
      241 tacgtggtga aggattcgcg cgacttcatc ttcaaggtgc gccgcccgga cgagtcggcg
      301 atgcagatcg ccgagaccgc gatgcgcgag gtgatcggca agaaccgcat ggacgcgatc
      361 ctctacgaga cccaggtcga cgtcgccaac aaggcgcgcg acctgatgca ggcgatccac
      421 gaccgctacg gcaccggcat ccagatcagc accgtgacga tccagaacgc ccagccgccc
      481 gagcaggtgc aggcggcgtt cgacgacgcg gtgaaggcgg gccaggaccg cgagcggcag
      541 aagaacgaag gccaggccta tgccaacgac gtcattccgc gcgcgcgcgg cacggcgtcg
      601 cgcctgctgc aggaggccga gggctacaag cagcgcgtga tcgcgagcgc cgagggtgac
      661 gcctcgcgct tcaagcaggt gctcgccgag tacgcgaagg cgccggccgt cacccgcgag
      721 cgcatctaca tcgacaccat gcagcaaatc ctgtcctcga ccaacaagat catgatggac
      781 taccgcggcc ccgggagcct gctgtacctg ccgctcgaca agatcatgca gcagtcgggc
      841 gcggggccgc tcaccgagtc gcaggccgcg cagcggccct ccgagccgac gccgccggag
      901 cccgggccgc gctcgcgcga gacgctccgc aaccgcgaca ggggggagcg ctagtgagag
      961 ccgaagggag tcgcccatga gggctctggg gccggtcgtc gccatcctga tcgtggtcgc
     1021 gctgctcgcg tcgggaacgc tgttcaccgt cgaccagcgc cagaacgcga tcgtgttcca
     1081 gctcggcgag gtgaccgacg tggtgcagcg cccgggcctg cacttcaagt ggccgctgct
     1141 gcagaacgtg cgcctgttcg acatgcgcat cctcaccttc gacgacgccg agccgctgcg
     1201 cttcctcacg cagggtaacc ggccggtgct ggtcgattcg ttcgtcaagt ggcgcatcgc
     1261 cgacgtcaag cagtactacg tgagcgtgca gggcgacgag tttcgcgcca tgacgcgcat
     1321 caagcagacc gtcgccggcg tgctgcgcga cgagttcggc gtgcgcacgg tgcacgaggt
     1381 ggtttcgggc gagcgcgagc agatcatggc gcgcgtgcgc gacaaggtcg accaggacct
     1441 caagcgcatc ggcgtcgaga tcatcgacgt gcgcctgaag cgcgtcgacc tgccgccgga
     1501 tgtcagtgag tcggtgtacc ggcgcatgga ggccgagcgc aagcgcatcg ccaacgagct
     1561 gcgctcgacc ggcgcggccg aggccgagaa gatccgcgcc gacgccgatc gtcagcgcga
     1621 ggtgatcctg gcggaggcgt tccgcgactc gcagcgcacc cgcggcgagg gcgacgccaa
     1681 ggcggcgaac atctacgccg gcgcgttctc gcagaacccc gagttcttct ccttctaccg
     1741 cagcatggag gcctaccgct cgaccttccg cggccgcaac gacctgatgc tgctcgaaac
     1801 caattccgat ttcctgcgct acttcaggga ttccctcgga cgagctcccg cgacgagaaa
     1861 atgagtgatg ggcaccttac tcttgatggc gctcgccctc atgctgatac tcgagggggt
     1921 gctgcccttc ctcgcgccta atctctggcg cgacacgttt cgccgcatca cccagatgag
     1981 cgacggccaa atccgcttcg tagggctgtc gtcgatgctg gtcgggctgc tgctgctcct
     2041 ctgggcgcga agctgacagt gaagtggctt ctcccggagt tcatcgagga catcctgccc
     2101 gccgaggcgg cgcgcatcga ggccttgcgc cgcaccatcc tggatctgtt gttcaagaac
     2161 aaattccagc tggtcatccc cccgctgctg gagtacatcg attcgctcct caccggcagc
     2221 gggcacgacc tggagttgcg caccttcaag gtggtggacc agctctcggg caggatgatg
     2281 ggtttgcgcg ccgacatcac gccgcaggtc gcgcgcatcg acgcgcacct cctcaaccgc
     2341 aggggcgtga cgcggctctg ctattgcggc gcggtgctgc atacgcggcc ggcgagcccg
     2401 ggcgcgacgc gcgagccgat gcagatcggc gccgagatct tcgggcacgc cggcctcgag
     2461 gccgacctc
//
LOCUS       DASPXW010000124         5202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_163901, whole genome shotgun sequence.
ACCESSION   DASPXW010000124 DASPXW010000000
VERSION     DASPXW010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5202
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163901"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VEL04_02955"
     CDS             <1..367
                     /locus_tag="VEL04_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HYT47357.1"
                     /translation="KEREAATLYQKSKDDLENARRSAALAARQSYLSVINGIAQVSAL
                     EQARVSSQSALASNKLGYEVGVRINIDVLNAQQQLYSTERDLAVARYNTITNQLRLKA
                     AAGSLRDEDLEEVNRALSL"
     gene            complement(345..1577)
                     /locus_tag="VEL04_02960"
     CDS             complement(345..1577)
                     /locus_tag="VEL04_02960"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HYT47358.1"
                     /translation="MRLLYTVCLRLALPFILARLWLRGRRERGYREAVTERFGMYATP
                     RPGKLIWLHAVSVGEARAAAPLVRALQELLPDHRMLLTCTTAAGRDTLQQLYGETVLA
                     AYLPYDFPESVQGFLEHFAPRLGVLIETEVWPNLLAGCAQRGVPVLLASARMSEKSAR
                     AYRRWRALTAPAFRALAAACAQSAADAERLRALGARHVEVTGNLKFDSRPDAAQLAAG
                     RAWRAALGRPVLLIASSREGEEALLLQALPENENILVVVVPRHPRRFDEVAQWAQSRR
                     TLQPSPAPAHRVYLGDTMGEMAFYYGACDVAVIGGSFAPLGGQNLIEALAAGAPVIVG
                     PSMFNFADATRLALEAGAALQSPDAATAVREALKLIEDSTRREAMGRAGKALCAEHRG
                     ATQRHLAVCKRIIETGPG"
     gene            complement(1574..2506)
                     /gene="waaC"
                     /locus_tag="VEL04_02965"
     CDS             complement(1574..2506)
                     /gene="waaC"
                     /locus_tag="VEL04_02965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HYT47359.1"
                     /translation="MPRILFVKTSSLGDVVHNCPAVSDAARKLPGAEIDWVVEQSFAG
                     VAAMHAAVRRVIPVAMRRWRAAPWNPAVWSEIGAFRRLLRAERYDAVIDTQSLLKSAL
                     VSAQSRGTRHGMDRASAREPLAARFYDVGHAVPRELHAVERNRRLSAAALGYTVDGSV
                     DYGLRAASDTPSKSAAPYMVLLTMTSRADKLWAEASWIELGRALATQVVLPWGSEAER
                     GRAERIAAAIGRASVPPRLQLEQLTGLFLKAGSVVGVDTGLTHLAAALGVPTVGVYCG
                     SDPALTGLYGAARAKNVGALGRAPQAAEVLKSLS"
     gene            2546..3313
                     /locus_tag="VEL04_02970"
     CDS             2546..3313
                     /locus_tag="VEL04_02970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYT47360.1"
                     /translation="MTAAPRAPLSAVLITRNAAAVLDACLESLAFADEIVLVDSASGD
                     GTAEIAQQRGARVVQKEWLGFGRQKQFAVDQAKHDWVLCLDADERVSPELAASIRAAL
                     ASPVSPVYRMARRNRFLGRWLAHGEGYPDWSPRLFNRQNARWSDDLVHEKVLFAVTPG
                     TLEGDLLHDSSDDLTAYLERQNRYTTLAARQAYERGLSAGIAHLLASPLVRFFKFYVL
                     RLGFLDGIPGLLHISIGCMNSYLKYAKLIELRKADSR"
     gene            3390..4340
                     /locus_tag="VEL04_02975"
     CDS             3390..4340
                     /locus_tag="VEL04_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYT47361.1"
                     /translation="MKVLVTGVAGFIGMHCAKRLLARGDEVIGIDNLSPYYSVQLKKD
                     RVAQLKHKAFTFRELDIAEAKSLQELFEKEKPQLVLHLAAQPGVRYSLENPAAYVQAN
                     VVGFANLLERCRRDAPRHLVFASSSSVYGANPKLPWSETDNVDHPISLYAATKKSNEL
                     MAHVYHRLHGLQITGLRYFTVYGPWGRPDMSPFLFARAIMEGSPIPVFNHGDMQRDFT
                     YVDDVVEGTLRVLDRPAAYAIYNIGNHAPVGLLDYIAALERAFGRKAKLEMKPMQPGD
                     VKSTYADTRALAKAVGFAPSTPLEEGLVRFAEWFKAYYGT"
     gene            4337..>5202
                     /locus_tag="VEL04_02980"
     CDS             4337..>5202
                     /locus_tag="VEL04_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47362.1"
                     /translation="MKKIYISNFSGLGNRLEALVLASMIQDRFGHSIFLDWPEKESLQ
                     IDGTGAGRIAPWERIGSVKLRDFDEPGLKALGKVRIINLRSTYGPRELQRRYVLPTAA
                     RLHPHPRISAAIRETLARFGDRPAVAVHIRQGDFHVSGDLYDATAHRHPAPALWWYEH
                     VMGAYVRAFPDVYFVLGHSGETQALARLQEMFEIVSLPGVFDYRGLFPGHRSTGHPVV
                     DLFGLASCTTLIATPTSSFSHWAANLLGPRTRTILPAPRTTRSAPSFGAAELWGCVVL
                     DWREAAEQGLRVA"
BASE COUNT          867 a         1797 c         1712 g          826 t
ORIGIN      
        1 caaggagcgg gaagccgcga cgctctacca gaaatccaag gacgacctgg aaaacgcgcg
       61 ccgctccgcc gcgcttgccg cccggcagtc atacctgtcg gtgatcaacg gcatcgcgca
      121 agtgagcgcc ctcgagcagg cgcgggtatc gtcgcaaagc gcgctcgctt ccaacaagct
      181 cggctacgag gtcggcgtgc gcatcaacat cgacgttctc aacgcgcagc agcagctcta
      241 ttccaccgag cgcgacctcg cggtggcgcg ctacaacacc atcaccaatc aactgcgcct
      301 gaaggccgcg gccggcagcc tgcgcgacga agacctcgaa gaggtcaacc gggccctgtc
      361 tctataattc ttttgcagac agcgaggtgc cgctgggtcg caccgcggtg ctcggcgcac
      421 agtgcttttc ctgcgcgtcc catcgcttca cgtcttgtgg aatcttctat taatttcaaa
      481 gcttctctga ctgccgtagc ggcatcgggg ctctgaagcg cggcgccggc ctcgagcgcg
      541 agccgggtgg cgtcggcgaa gttgaacatg ctcggcccga cgatgaccgg cgcgccggcg
      601 gcgagcgcct cgatcaggtt ctggccgccg agcggcgcga acgagccgcc gatcacggcg
      661 acatcgcaag cgccgtaata gaacgccatc tcgcccatcg tgtcgccgag gtacacgcgg
      721 tgcgcgggcg caggcgaggg ctgcagtgtg cggcgtgact gcgcccactg cgccacctcg
      781 tcgaagcgcc ggggatggcg cggcacgacg acgacaagga tgttttcgtt ctcgggcagc
      841 gcctgcagaa gcagggcttc ctcgccctcg cgcgagctcg cgatcagcag caccggccgg
      901 ccgagcgcgg cgcgccaggc gcggccggcg gcgagctgcg cggcatcggg tcggctgtcg
      961 aacttgaggt tcccggtcac ttcgacatgg cgcgcgccga gcgcacgcag ccgctcggca
     1021 tcggcggcgc tctgcgcgca ggcggcggcg agggcgcgaa acgccggcgc cgtcagcgcg
     1081 cgccagcgcc ggtaggcgcg cgccgacttc tccgacatgc gcgcgctcgc gagcagcacg
     1141 gggacgccgc gctgcgcgca gccggcgagc aggttcggcc agacctcggt ctctatcagc
     1201 acgcccaggc gcggcgcgaa atgctcaaga aagccctgca ccgactcggg aaagtcgtag
     1261 ggcagatacg cggcgagcac cgtctcgccg tagagctgct gcagcgtgtc gcgcccggct
     1321 gcagtggtgc aggtgaggag catgcggtgg tcgggcaaca gttcctggag cgcgcgaacc
     1381 agcggcgccg cggcgcgcgc ctcccctacg gaaaccgcat gcagccagat gagcttgccg
     1441 ggcctcggcg tggcatacat gccgaagcgc tcggtgaccg cctcgcggta gccgcgctcg
     1501 cggcgtccgc gcagccacag ccgcgccagg atgaacggca gcgcgagccg caggcagacc
     1561 gtatagagga gcctcacgaa agcgacttca gcacttcggc agcctgcggc gcccggccca
     1621 atgcgccgac atttttcgcc cgtgccgcgc cgtagagccc ggtgagcgcg ggatcggagc
     1681 cgcaatagac tccgaccgtc ggcacgccca gcgcggcggc gagatgagtg agcccggtgt
     1741 cgacgcccac gacgctcccg gctttcagaa aaagccccgt cagctgctcg agctggagcc
     1801 ggggcggcac gctcgcgcgt ccgatcgcgg cggcgatgcg ctcggcccga ccgcgctcgg
     1861 cttcgcttcc ccagggcagc acgacttgcg tggcgagcgc gcggccgagc tcgatccagc
     1921 tcgcctccgc ccagagcttg tcggcacggc tcgtcatcgt cagcaggacc atataaggag
     1981 cagcgctttt tgacggagta tcgcttgcgg cgcgaagtcc gtagtcgacc gatccatcaa
     2041 ccgtgtaacc caatgcagcg gcgctcaggc gccggttgcg ttccaccgca tgcagctcgc
     2101 gcggcaccgc gtgcccgacg tcgtagaagc gcgcggcgag cggctcccgt gcgctcgcgc
     2161 ggtccatgcc gtggcgcgtg ccgcgagatt gggccgagac gagcgcgctc ttcaggagcg
     2221 actgcgtgtc gatcaccgcg tcgtagcgct cggcgcgcag cagccggcgg aaagcgccga
     2281 tctcggacca gaccgcggga ttccagggcg cggcgcgcca gcgcctcatc gccaccggga
     2341 tcacccgccg taccgcggcg tgcatcgcgg cgacgccggc gaaggactgt tccaccaccc
     2401 aatcgatctc cgcacccgga agcttgcgcg ccgcgtcgct caccgccggg cagttgtgga
     2461 ccacgtcccc gagcgacgag gtcttgacga agaggatgcg cggcattggc tagaataggg
     2521 cccgcaaatt ctacgagata atgatttgac cgccgcgccc cgcgccccgc tttcggccgt
     2581 cctcatcacc cgcaacgccg ccgccgttct cgatgcctgt ctcgagagcc tggcgttcgc
     2641 cgacgagatc gtgctggtcg attcggcgag cggcgacggc accgccgaga tcgcgcagca
     2701 gcgcggcgcg cgcgtagtgc agaaggaatg gctcggcttc ggccgccaga agcagttcgc
     2761 ggtcgaccag gcgaagcacg actgggtgct gtgtctcgac gccgacgagc gtgtctcgcc
     2821 cgagctcgcg gcgagcatcc gcgcggcgct cgcctctccg gtttctcccg tctatcgcat
     2881 ggcgcggcgc aaccgcttcc tcgggcgctg gctggcgcac ggcgagggct atcccgactg
     2941 gagcccgcgg ctcttcaacc gccagaacgc gcgctggagc gacgacctgg tgcacgagaa
     3001 agtgctgttc gcggtcacgc cgggcacgct cgagggcgac ctgctgcatg actcctccga
     3061 cgacctcacg gcttatctcg agcgccagaa ccgctacacc acgctcgccg cgcgccaggc
     3121 ctacgagcgc gggctcagcg ccgggatcgc gcacctgctc gcctcgccgc tggtgcgctt
     3181 cttcaagttc tacgtgctgc gcctcggctt tctcgacggc attccgggac tgctgcacat
     3241 ctcgatcggc tgcatgaaca gctacctgaa gtacgccaag ctgatcgagc tgcgcaaagc
     3301 ggacagccgg tgaaaatggg gacggacccc attttttcgc aatcgcgaga tgagaaaaat
     3361 ggggtctgtc cccattaaag tccttcccca ttaaagtcct cgtcactggc gtcgccggct
     3421 tcatcggcat gcactgcgcg aaacggctcc tcgcgcgcgg cgacgaagtt atcggcatcg
     3481 acaatctttc gccctattac tcggtgcagc tgaagaagga ccgcgtcgcg cagctcaagc
     3541 acaaagcctt tacgtttcgc gagctcgata tcgccgaggc gaagtccctg caggaacttt
     3601 tcgaaaaaga aaagccgcag ctggtgctgc acctcgcggc gcagccgggg gtgcgctact
     3661 cgctcgagaa cccggcggcc tacgtgcagg cgaatgtggt cggcttcgcc aacctcctcg
     3721 agcgctgccg gcgcgacgcg ccgcggcacc tggtgttcgc ttccagctcg agcgtgtacg
     3781 gcgcgaaccc gaagctgccg tggtcggaaa cggacaacgt cgaccatccg atcagcctgt
     3841 acgccgccac gaagaaatcc aacgagctga tggcgcacgt ctaccaccgg cttcacgggc
     3901 tgcagatcac cgggctgcgc tatttcacgg tctatggtcc ctggggcagg ccggacatgt
     3961 cgccgttttt gttcgcgcgc gccatcatgg aaggcagtcc gatcccggtc ttcaaccatg
     4021 gcgacatgca gcgcgacttc acctacgtgg acgacgtcgt cgaaggcacg ctgcgcgtgc
     4081 ttgatcggcc ggcggcctac gcgatctaca acatcggcaa ccacgcccct gtcgggctgc
     4141 tcgactacat cgccgcgctg gagcgcgcct ttggcaggaa agccaagctg gagatgaagc
     4201 cgatgcagcc cggcgatgtg aagtccacct acgccgatac gcgcgcgctc gccaaggcgg
     4261 tcggcttcgc gccgtcgacg cccctggaag agggcttagt gcgcttcgcc gagtggttca
     4321 aggcctacta tggaacatga aaaaaatcta tatctcgaat ttctccggac tgggcaatcg
     4381 cctcgaggcg ctggtgctcg cgagcatgat ccaggaccgc ttcggccact cgatttttct
     4441 cgactggccc gagaaggaga gcttgcagat agacggcacc ggcgcgggcc gcatcgcgcc
     4501 atgggagcgc atcggcagcg tcaagctgcg tgatttcgac gagccggggt tgaaggcgct
     4561 gggcaaggtc cgcatcatca acctgcgctc cacctacgga ccgcgcgagc tgcagcgccg
     4621 ctacgtgctg cccacggcgg ctcgcctgca cccgcatccg cgcatttccg cggccattcg
     4681 cgagacgctg gcgcgctttg gcgaccggcc ggcggttgcg gtccacatcc ggcagggcga
     4741 tttccacgtc tcgggcgatc tttacgacgc gaccgctcac cgccatccgg cccccgcgct
     4801 ttggtggtac gagcacgtga tgggcgccta tgtccgggcg ttcccggacg tgtacttcgt
     4861 gctgggccat agcggggaga cgcaggcgct ggcacgactg caggagatgt tcgagatcgt
     4921 gtccttgccc ggcgtgttcg attaccgggg actattcccc ggccatcgct ccaccggcca
     4981 tccggtggtc gacctgttcg gactggcaag ctgcaccacg ctcatcgcca cgccgacctc
     5041 gagcttttcg cattgggctg caaatctgct cggtcctcga acgcgaacca tcctgccggc
     5101 gccgcgcacc acgcgcagcg cgccgagctt cggcgcggcc gagctctggg ggtgcgtggt
     5161 gctcgactgg cgtgaagctg cggaacaggg cttgcgcgtc gc
//
LOCUS       DASPXW010000125         4539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1173255, whole genome shotgun sequence.
ACCESSION   DASPXW010000125 DASPXW010000000
VERSION     DASPXW010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173255"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..484)
                     /locus_tag="VEL04_02985"
                     /pseudo
     CDS             complement(1..484)
                     /locus_tag="VEL04_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015602156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
     gene            557..2251
                     /gene="xdhB"
                     /locus_tag="VEL04_02990"
     CDS             557..2251
                     /gene="xdhB"
                     /locus_tag="VEL04_02990"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HYT47363.1"
                     /translation="MPGERGEMLVHASTQHPTEVQHLVAAALGVPDATVTVELRRLGG
                     GFGGKETQAAVYAIAAALLARATGRPVKLRADRDDDIAGTGKRHDFLYAYDAGFDERG
                     GIEALDLMLASRCGISADLSGAVNDRAVFHCDNGYFLPNVRITSHRCKTNTVSNTAFR
                     GFGGPQGMFAIEGVIAAIARRLGVDPLEVRKRNLYGEAPRNVTHYGMTLKDNVLPGLI
                     GELETRSEYRKRRSFVTDWNKKNELIKRGLALTPVKFGIAYTATQMNQGGALVHLYTD
                     GSVHLNHGGTEMGQGLLIKVAQIVAEEFQIDVARVHITATSTGKVPNTSPTAASSGTD
                     INGEAARAACRKLKARLAQFCQEKYGVKPETLGFAQLVREAYQARVPLSATGYWRTPE
                     IHFDRERFQGEPFYYFAYGAAVSEVAVDTLTGESRLLRADLLHDVGRSLNPAVDRGQV
                     EGGFIQGMGWLTMEELVWDAKGALLTHAPSTYKIPTASDCPAAFNVWLWERGENVRDV
                     VYRSKAVGEPPLMLALSVFHAIYDAVGATSTSRDPVPLEAPATPENTLKAIYAMRD"
     gene            2235..3014
                     /gene="xdhC"
                     /locus_tag="VEL04_02995"
     CDS             2235..3014
                     /gene="xdhC"
                     /locus_tag="VEL04_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory protein XdhC"
                     /protein_id="HYT47364.1"
                     /translation="MQCVIEILVVETRGSAPREAGTRMWLSASEARGTIGGGNLEYTA
                     LKIAREMLLSGETQRERRFALGDSLGQCCGGAVKLAFSALSSFPVEKSDIQFHVVLFG
                     AGHVGKELARILERLPCRLTWIDPRPDVFPSSVNEKVRVVIEEEPAWMVDEAPRGAYY
                     LVMTHSHALDLEIVERVLRRGDFAFLGLIGSETKAAKFRARLKARGLDSSKLISPIGL
                     FKAGKHPAEVAVAAAAQLLALQSPLMAASRIISDQREPSAR"
     gene            complement(2980..4146)
                     /locus_tag="VEL04_03000"
     CDS             complement(2980..4146)
                     /locus_tag="VEL04_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HYT47365.1"
                     /translation="MEAYLLDWLNLVVRWAHIVVAIAWIGASFYFIWLDDKLEVSGEA
                     ELAGELHAIHGGGFYRVQKYRLAPGVLPRQLHWFKWEAYWTWITGFILLVLIYYLGAE
                     LYLIDPAVMKLSNATAIGFGLGSLLVGLVVYEGLCRSPLAKNELIFSLVIFVLLAFAA
                     WGLTQVFSGRGAFIHYGAILGTIMAANVAHVIIPGQRELVRAKEQGREPDPKYALAGK
                     QRSVHNTYFTLPVIFTMISGHHAMTFGGRWNWLVLVALTLAGALIRVWFVQRHKGRAQ
                     AWVWIASVILVIAIAWLLAPKAQPPAEPVSFAEVNQVIHARCLVCHAEKPAFPGLAEA
                     PKGVRLDTPERVRAQAQPIYQQTALARAMPPGNITGMTEDERAVIARWVRAGPR"
     gene            complement(4148..4462)
                     /locus_tag="VEL04_03005"
     CDS             complement(4148..4462)
                     /locus_tag="VEL04_03005"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: protein motif:HMM:NF012785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HYT47366.1"
                     /translation="MVDVSRGVVAAGMKVELHGPQGGIVAAGITNAKGLLENQANMPA
                     GFYEARFHVAAWYREQGVALPQVPFLDVVHFRFGISDPRQHYHLPFKCTPWGYSCFRG
                     GA"
BASE COUNT          798 a         1530 c         1495 g          716 t
ORIGIN      
        1 cgtagaggtt gcgcaggcgc acctcgagcg gatcgaggcc caggcggcgc gcgatcgcgg
       61 cgatcacgcc ctcgatcgcg aacatgccct gcggtccgcc gaagccgcgg aaggcggtgt
      121 tcgacaccgt gttggtcttg cagcgatgcg aggtgatgcg caggtgcggc aggaaatagg
      181 cgttgtcgca gtggaacacc gcgcggtcgt tgaccgcgcc ggaaaggtcg gccgagatgc
      241 cgcagcgcga cgccagcatc aggtcgagcg cctcgatgcg ccccgcttga tcgaagccgg
      301 cttcgtactc gtacagaaaa tcatggcgtt tgccggtgcc gatcatgtcg tcgtcgcggt
      361 cggcgcgcag cttcaccggc cggccggtgg cgcgcgcgag aagcgcggca gcgatcgcgt
      421 atacggcggc ctgcgtctcc ttgccgccga agccgccgcc gaggcggcgc agctctaccg
      481 tgactgcggc atcgggaaga tcgagcgctg cggcggccag gagcacttct atctcgaggg
      541 ccaagtcgcg ctggcgattc ccggggaacg cggcgagatg ctggtgcatg cgtcgaccca
      601 gcacccgacc gaggtgcagc atctcgtcgc cgccgcgctc ggcgtgccgg acgcgacggt
      661 gaccgtcgag ctgcgccgcc tgggcggcgg cttcggcggc aaggagacgc aggccgcggt
      721 atacgccatc gcggcggcgc tgctcgcgcg cgccactggc cgcccggtga agctgcgcgc
      781 cgaccgcgac gacgacatcg ccggcaccgg caagcggcac gacttcctgt acgcatacga
      841 cgccgggttc gacgagcgcg gcggcatcga ggcgctcgac ctaatgctcg cctcgcgctg
      901 cggcatctcg gccgacctct ccggcgcggt gaacgaccgc gcggtgttcc actgcgacaa
      961 cggctacttc ctgcccaacg tgcgcatcac ctcgcaccgc tgcaagacca acacggtctc
     1021 caacaccgcg ttccgcggct tcggcgggcc gcagggcatg ttcgccatcg agggcgtgat
     1081 cgccgcgatc gctcggcgcc tcggcgtcga tccgctcgag gtgcgcaagc gcaacctcta
     1141 cggcgaggcg ccgcgcaacg ttacgcacta cggcatgacc ctcaaggaca acgtgctgcc
     1201 cgggctgatc ggcgagctcg agacgcgctc ggaataccgc aagcgccgct cgtttgttac
     1261 tgattggaat aagaagaatg aattgataaa acgcggcttg gccttaaccc ccgtcaagtt
     1321 cggcatcgcg tataccgcga cacagatgaa ccagggcggg gcgctggtgc acctctacac
     1381 cgacggcagc gtgcacctca accatggcgg caccgagatg ggccagggac tgctcatcaa
     1441 ggtggcgcag atcgtggccg aggaattcca gatcgacgtc gcgcgcgtgc acatcaccgc
     1501 gacctccacc ggcaaggtgc cgaacacctc gcccaccgcc gcctcgagcg gcaccgacat
     1561 caacggcgag gcggcgcgcg ccgcctgccg caagctgaag gcgcgcctgg cgcagttctg
     1621 tcaggagaaa tacggtgtga aaccggagac cctgggcttt gcccagctgg tgcgcgaggc
     1681 ctaccaggcg cgcgtgccgc tctcagccac cggctactgg cgcacgccgg agatccattt
     1741 cgaccgcgag cgcttccagg gcgagccgtt ctactacttc gcgtacggcg cggcggtgtc
     1801 ggaggtggcg gtcgacacgc tcaccggcga gtcgcgcctg ctgcgcgccg acctgctgca
     1861 cgacgtcggc cgctcgctca accccgcggt cgaccgcgga caggtcgaag gcggtttcat
     1921 ccaggggatg ggctggctga cgatggagga gctggtgtgg gatgcgaagg gcgcgctcct
     1981 cacccacgcg ccttcgacgt acaaaatccc cacggcgagc gactgcccgg ccgctttcaa
     2041 cgtctggctg tgggagcgcg gcgagaatgt gcgcgatgtc gtctaccgct cgaaggccgt
     2101 gggcgagccg cctctgatgc tggcgctttc ggtattccat gcgatctacg acgcggtcgg
     2161 cgcgacgagc acttcgcgcg acccagtgcc gctcgaagcg cctgcgacac cggaaaacac
     2221 tctcaaggcc atctatgcaa tgcgtgattg aaatcctggt cgtcgagact cgcggctcgg
     2281 cgccgcgcga ggccggcacg cgcatgtggc tgagcgccag cgaagcgcgc ggcaccatcg
     2341 gcggcggcaa cctcgagtac accgcgctca agatcgcgcg cgagatgctg ctctccggcg
     2401 agacgcagcg cgagcgccgc ttcgcgctcg gcgattctct cggccaatgc tgcggcggag
     2461 ccgtaaaact cgcttttagc gcactttcat ctttccccgt ggaaaagagt gacattcaat
     2521 tccacgtcgt tctcttcggc gcggggcatg tcgggaaaga gctcgcgcgc atcctcgagc
     2581 gcctgccgtg caggctcacc tggatcgacc cgcgtcccga cgtgtttcct tcttcagtca
     2641 atgagaaggt ccgcgtggtg atcgaggaag agcccgcctg gatggtcgac gaggcgccgc
     2701 gcggcgcgta ctacctggtg atgacgcaca gccacgcgct cgacctcgag atcgtcgagc
     2761 gcgtgctgcg gcgcggggac ttcgctttcc tcggcctgat cggatcagaa accaaggcgg
     2821 cgaagtttcg cgcccgcctg aaggcgcgcg gcctggattc ctcgaaactg atttcaccga
     2881 tcgggctttt caaggcgggc aagcatccgg ccgaggtcgc cgtagccgcc gcggcgcaac
     2941 tgctcgcgct tcagtcgccc ttgatggctg cgtcgcgcat catctcggac cagcgcgaac
     3001 ccagcgcgcg ataaccgcgc gctcgtcctc ggtcatgccg gtgatgttcc ccggcggcat
     3061 cgccctggcg agcgccgtct gctggtagat cggctgcgcc tgggcgcgca cgcgctccgg
     3121 cgtatcgagc cgcacgccct tgggcgcctc ggcgagcccc gggaaagccg gcttctcggc
     3181 gtggcagacg aggcagcgcg cgtggatgac ctggttcacc tctgcgaaac tcaccggctc
     3241 ggcgggcggc tgcgccttag gcgccagcag ccaggcgatc gcgatcacca gaatcacgct
     3301 cgcaatccag acccatgctt gcgcccggcc cttgtgccgc tgcacgaacc agacccggat
     3361 caacgcgccg gccagcgtca gcgcgacgag caccagccag ttccagcggc cgccgaaggt
     3421 cattgcatga tggcccgaga tcatggtgaa gatcaccggc agcgtgaagt aggtgttgtg
     3481 cacgctgcgt tgcttgcccg cgagcgcgta tttcgggtcg ggctcgcggc cctgctcctt
     3541 cgcccgcacc agctcgcgct gtccgggaat gatgacgtgc gcgacgttgg cggccatgat
     3601 cgtgccgagg atcgcgccgt aatggatgaa ggcgccgcgg ccgctgaaca cctgtgtgag
     3661 accccaggct gcgaacgcca gcaggacaaa aatcaccagg ctgaaaatca attcattttt
     3721 ggccagcggc gagcggcaca gtccttcgta caccaccagg ccgacgagca ggctgccaag
     3781 gccgaagccg atcgcggtgg cgttcgaaag cttcatcact gcgggatcaa tcaggtaaag
     3841 ctcggcaccg aggtaataaa tcagaaccaa aagaatgaac cccgtgatcc aggtccagta
     3901 ggcctcccat ttgaaccagt ggagctgtcg cggcagcacg cccggcgcaa ggcggtactt
     3961 ctgcacgcgg tagaagcccc cgccgtggat cgcatgcagc tcgccggcga gctcggcttc
     4021 gcctgacacc tcgagcttgt cgtcgagcca gatgaagtaa aacgacgccc cgatccaggc
     4081 gatcgccacc acgatgtgcg cccagcgcac gaccaggttc agccagtcga ggagataggc
     4141 ctccaatcta ggcaccgccc cggaagcacg aatagcccca gggcgtacat ttgaacggca
     4201 ggtggtaatg ctggcgcggg tcggagatgc cgaagcggaa atgcaccacg tcgagaaaag
     4261 ggacctgcgg cagcgctacg ccctgctcgc ggtaccacgc ggcgacgtga aaccgcgcct
     4321 cgtagaagcc cgcgggcatg ttcgcctggt tttcgagcag gccttttgcg ttcgtgatgc
     4381 cagccgcgac gatcccgcct tgcggcccgt gcagctcgac tttcatgccc gcggcgacga
     4441 cgccccgtga tacatcgacc acgtgaatgg aaatacccgg cacagggaaa tcgctagttg
     4501 agcttggccc cggaatcctt cactggctgg taccactcc
//
LOCUS       DASPXW010000126         5386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1048707, whole genome shotgun sequence.
ACCESSION   DASPXW010000126 DASPXW010000000
VERSION     DASPXW010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5386
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048707"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VEL04_03010"
     CDS             <1..573
                     /locus_tag="VEL04_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47367.1"
                     /translation="LKETAKENSVILVADVDMLADGAAVDVQEVFGRKIVVPSNGNLA
                     LALGMVEQFAAGDDLISLRSRSASFRPLTVVRELEAEAQREYFGKIQALEGELQKTTA
                     KLQELQKAQGSGAGKSGQILTPEQQAELERFRKTVAEARLALKEVRKNLRQDAESLVF
                     WTKLANIVLMPLLVALAGLLIAFIRKTRTA"
     gene            570..1586
                     /locus_tag="VEL04_03015"
     CDS             570..1586
                     /locus_tag="VEL04_03015"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HYT47368.1"
                     /translation="MNARVAAILVVLLVVLGGAALLFQREESARRPENVATLGQPLLK
                     QLPAAAIAQIRIVEPKATLTLQRKEGGWVIAERGGFPADIGKVREFVLKMIELKIGQS
                     EPAAERDRARLNLDASGTQVEFGGADGKTLGRLIVGKKYFRREVDNPDRAAADGRFVG
                     LPGEAKLVYIVSDPLAQASARSADWIDRASFQVEKVKALEVRYPAGGGWRIERGGENA
                     PWKLAAAKPGEKLDSGRANAATYSLSLLELADVAPQDAKDAGLDTPTLINATTLDGRS
                     YAIRVGKLAGEDYYVAFRASGEPVKPREKLLSDHLLLMPKSKLADTLKKRSELLEKKE
                     GTRK"
     gene            1613..2053
                     /locus_tag="VEL04_03020"
     CDS             1613..2053
                     /locus_tag="VEL04_03020"
                     /inference="COORDINATES: protein motif:HMM:NF022479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HYT47369.1"
                     /translation="MALEPHIPEIAGVIQLAVAPVFMLTAVGTLIAALNIRLGRAVDR
                     RRVLEQELPAMPDDEIGPARDELATIARRIRFVYFAITAAVVSGLFICLLIAGAFLAT
                     FVKTDISYTIAVLFVFAVVALIACLLLFLREIFLAVSTPRHVPR"
     gene            2083..4128
                     /gene="fusA"
                     /locus_tag="VEL04_03025"
     CDS             2083..4128
                     /gene="fusA"
                     /locus_tag="VEL04_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HYT47370.1"
                     /translation="MTHSIKRIRTIALVGQAGAGKTALAEALLARAGAIPAPGSVERG
                     TTVCDYTPLEKQLRHSLKLAVASFDFQETKIHLLDTPGYPDFLGHALPALAAVETAAV
                     VINAQNGIEMMTGRFMQWAAKRGLDRLVVVNKIDAENVDLEGLLERIQQAFGKECLPL
                     NLPAASHSRVSDCFFAPSGEADFSSVEEAHRKLIDQVVEVDEKLMERYLEKGEVTPEE
                     LHEPLERALREGHLIPVVFTSAKSGAGVAELLEVVEKLLPNPAEGNPPAYLSTPAGGG
                     APTELQPVPDPGKHVLAHVFKIEIDPYIGRLAVFRMHQGRLTPGTQLYVGEGRKPIKP
                     GHLYLLRGKTQTEVHEAIPGDICAIAKIEEIQFDHVLHDAASDAHVHARPLELPTSVF
                     GLAVQPKKRGDEQKVSDVLHKIMAEDPCFRIEHNAQANETVMRGLGEMHLRTVLEKMS
                     SQYKIDLDTRPPAIPYRETIGARAEGHSRHKKQTGGAGQFGEVYLKVEPLPRGSGFEF
                     VDSVKGGSIPNQFIPSVEKGVRSVLDSGFVAGYPLQDVRVIVYDGKSHPVDSKDVAFM
                     SAGRKAFLDALGKARPIVLEPIVNVEIVAPEERMGDIAGELSGHRGQIKGSDAPRPGL
                     VQITAQAPLSELEHFPARLKSITAGHGSYSLEFSHYEPAPPQLQQKLVAAHRPAAEE"
     gene            4141..4914
                     /locus_tag="VEL04_03030"
     CDS             4141..4914
                     /locus_tag="VEL04_03030"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYT47371.1"
                     /translation="MRAGRGAPPLVFVHGFACSHADWQFQFDFFSNTNEVVACDLRGH
                     GETPGRPHECSIEHYGGDVAALITNLDFSKVILIGHSMGCRVVLEANRLAPDRVAGIV
                     LVDGSRLASGDPDAAESAARAATEQSGYPAFAENLFRQMFFSPSAFADAIVARAVRTS
                     ALTGPALWPRMARWDAGEMDAALAAVRTPLLAIQSTTRDAQLRRSPLKPGDTSPWLEL
                     LKKSIPGAKIEIIPGVGHFPQLEAAETVNRLIAGFVQKP"
     gene            complement(4902..5003)
                     /locus_tag="VEL04_03035"
                     /pseudo
     CDS             complement(4902..5003)
                     /locus_tag="VEL04_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006404903.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
BASE COUNT          933 a         1834 c         1778 g          841 t
ORIGIN      
        1 ttgaaagaaa ccgccaagga aaactcggtg atcctggtcg cggacgtcga catgctggcc
       61 gacggcgccg cggtcgacgt gcaggaggtc ttcggccgca agatcgtcgt gccttccaac
      121 ggcaacctcg cgctcgcgct cggcatggtg gagcagttcg ccgcgggcga cgacctgatc
      181 tcgctgcgca gccgcagcgc ctcgttccgc ccgctcacgg tggtgcgcga gctggaggcc
      241 gaggcgcagc gcgaatactt cggcaagatc caggcgctgg aaggcgagct gcagaagacc
      301 accgccaagc tgcaggagct gcagaaagcg caaggcagcg gcgccggcaa gtcgggccag
      361 atcctcaccc ccgagcagca ggcggagctc gagcgcttcc gcaagaccgt ggccgaggcg
      421 cggctcgcac tgaaggaagt gcgcaagaac ctgcgccagg acgccgagag cctggtgttc
      481 tggaccaagc tggcgaacat cgtgctgatg ccgctgctgg tcgcgctcgc gggtttattg
      541 attgctttca tcagaaaaac aagaacggca tgaacgcccg ggtggcggcg atcctggtgg
      601 tgctgctggt ggtgctgggc ggcgcggcgc tgctcttcca gcgcgaggag agcgcgcgcc
      661 ggccggagaa cgtcgccacg ctcgggcagc cgctgctcaa gcagctgccg gcagccgcca
      721 tcgcgcagat ccgcatcgtc gagccgaagg ccacgctcac cctgcagcgc aaggagggcg
      781 gctgggtgat cgccgagcgc ggcggattcc ctgccgatat cggcaaggtg cgcgaattcg
      841 tcctcaagat gatcgagctg aagatcggcc agagcgagcc ggcggccgag cgggatcgcg
      901 cccggctcaa cctggacgcg tccgggaccc aggtcgagtt cggcggcgcg gacggcaaga
      961 cgctcgggcg cctgatcgtc ggcaagaaat acttccggcg cgaggtcgac aatcccgacc
     1021 gcgcggcggc cgatggccgc ttcgtcggcc tgcccggcga ggccaagctg gtctatatcg
     1081 tctccgatcc gctggcgcag gcgagcgcca ggtccgccga ctggatcgac cgcgcctcgt
     1141 tccaggtgga gaaggtgaag gcgctcgaag tccgctaccc cgcaggcggc ggctggcgca
     1201 tcgagcgcgg cggcgagaac gcgccctgga agctcgccgc tgcgaagccc ggcgagaagc
     1261 tcgactcggg cagggcgaac gccgccacgt actcgctcag cctgctcgag ctcgcggacg
     1321 tggcgccgca ggacgcgaag gacgccgggc tcgacacgcc gacgctcatc aacgccacca
     1381 cgctcgatgg ccgctcctac gccatccgtg tcggcaagct cgcgggcgag gactactacg
     1441 ttgcgttccg cgcttcgggc gagcctgtga agccgcgcga gaagctgctt tcggatcatc
     1501 tgttgctcat gcccaagtca aaattggccg atacgctgaa gaagcgctcg gagcttctcg
     1561 agaagaagga aggcacgagg aaatagaaaa accgtcctgc tagacttggc cggtggcact
     1621 cgagccgcat atcccggaaa tcgccggggt gatccagctc gcggtcgcgc cagtgttcat
     1681 gctgaccgcg gtgggcaccc tcatcgccgc gctcaacatc cggctcggac gggcggtcga
     1741 ccggcgccgc gtcctcgagc aggagctgcc ggcgatgccc gacgacgaga tcggcccggc
     1801 gcgcgacgag ctcgccacca tcgcgcggcg catccgcttc gtctatttcg cgataacagc
     1861 cgcggtggtt tcggggctgt tcatctgcct gctcatcgcc ggcgccttcc tcgccacctt
     1921 cgtgaagacc gacatctcct ataccatcgc cgtcctgttc gtcttcgcgg tggtggcgct
     1981 gatcgcctgc ctgctgctgt tcctgcgcga aatcttcctg gctgtctcga ccccgcggca
     2041 cgtgccgcgg taagatcaat ttcgccagag gaggcggccc ccgtgactca ttcgatcaaa
     2101 cgcatcagga ccattgcgct cgtcggccag gcgggagcgg gaaagaccgc cctggccgag
     2161 gcgctgctcg cgagagcggg ggcgatcccc gcgccgggca gcgtcgagcg cggcactacc
     2221 gtgtgcgact acaccccgct cgagaagcag ctgcggcact ccctcaagct cgcggtggcg
     2281 agcttcgatt tccaggaaac aaaaattcat ttgctggata ccccgggcta cccggacttt
     2341 ctgggccatg ccctgcctgc cctggcggcg gtggaaaccg ccgccgtagt catcaacgca
     2401 cagaacggca tcgagatgat gaccgggcgg ttcatgcagt gggcggcaaa gcgcgggctc
     2461 gaccggctgg tggtggtcaa caagatcgac gccgagaacg tcgacctgga agggctgctc
     2521 gagcgcatcc agcaggcgtt cggcaaggaa tgcctgccgc tcaacctgcc tgcggccagc
     2581 cacagcaggg tgtcggactg cttcttcgca ccctcgggtg aagccgattt ttcctcggtc
     2641 gaagaagcgc accgcaagct gatcgaccag gtggtggaag tcgacgagaa gctgatggaa
     2701 aggtatctgg agaaaggcga ggtgacgccc gaagagctgc acgagccgct cgagcgcgcg
     2761 ctgcgcgagg gccatctcat cccggtggtg ttcacctcgg cgaaaagcgg cgccggcgtc
     2821 gccgagctgc tcgaggtggt ggagaagctg ctgcccaatc ccgccgaagg caacccgccc
     2881 gcctatctca gcacgccggc cggcggcggc gcaccgaccg agctgcagcc ggtccccgac
     2941 ccgggcaagc acgtgctcgc ccacgtcttc aagatcgaga tcgatcccta catcgggcgc
     3001 ctggcggtgt ttcgcatgca ccaggggcgt ctcacgcccg gcacccagct ctacgtcggc
     3061 gaaggccgca agccgatcaa gcccgggcat ctctacctgc tgcgcggcaa gacccagacc
     3121 gaggtgcacg aggcgatccc gggcgacatc tgcgccatcg ccaagatcga ggagatccag
     3181 ttcgaccacg tgctgcacga tgcggcttcc gacgcgcacg tgcacgcgcg gcccctcgag
     3241 cttcccacct cggtgttcgg cctcgcggtg cagccgaaga agcgcggcga cgagcagaag
     3301 gtgtccgacg tgctgcacaa gatcatggcc gaggacccct gcttccgcat cgagcacaac
     3361 gcgcaggcga acgagaccgt gatgcgcggc ctgggcgaaa tgcacctcag gacggtgctg
     3421 gagaagatgt catcgcagta caagatcgac ctcgacaccc ggccgccggc catcccgtac
     3481 cgcgagacca tcggggcgcg cgccgaaggc cacagccgcc acaagaagca gaccggcggc
     3541 gccggccagt tcggcgaggt ctacctgaaa gtggagccac tgcctcgggg gagtggcttc
     3601 gaatttgtcg acagcgtgaa gggcggctcg atcccgaacc agttcattcc ctcggtcgaa
     3661 aaaggcgtgc gcagcgtgct cgactcgggc ttcgtcgccg gctacccgct gcaggacgtg
     3721 cgcgtcatcg tgtacgacgg caaaagccac ccggtcgatt ccaaggacgt agccttcatg
     3781 tcggccgggc gcaaggcgtt cctcgacgcg ctcggcaagg cgcggccgat cgtgctcgag
     3841 ccgatcgtca acgtcgagat cgtcgccccg gaggaacgca tgggcgacat cgccggcgag
     3901 ctctccggcc accgcggcca gatcaagggc tccgatgcgc cgcgtccggg cctggtgcag
     3961 atcaccgcgc aggcgccgct ctccgagctc gagcactttc cggcgcggct caaatcgatc
     4021 accgcgggcc acggctccta cagcctggag ttcagccatt acgagcctgc gccgccgcag
     4081 ctgcagcaaa agctggtcgc tgcgcaccgg cccgccgccg aggagtaagc cttgcactac
     4141 gtgcgcgccg gccgcggcgc gccgccgctc gtcttcgtgc acggtttcgc ctgcagtcac
     4201 gcagactggc agttccagtt cgatttcttt tcgaacacga atgaagtcgt tgcgtgcgac
     4261 ctgcgcggcc acggcgagac gcccggccgg ccgcacgagt gctcgatcga gcactacggc
     4321 ggcgatgttg ccgccctgat aacgaatcta gacttctcca aagtaattct tattggtcac
     4381 agcatgggtt gtcgcgtggt gctcgaggcg aaccgcctcg ctcccgaccg ggtcgcgggt
     4441 atcgtcctcg tcgatggcag ccgcctcgcc agcggcgacc cggacgcggc cgagagcgcc
     4501 gcgcgcgccg cgaccgagca atccggctac cccgcgtttg ccgagaacct cttccggcag
     4561 atgttcttca gcccctcggc tttcgccgac gcaatcgtcg cccgcgcggt gcggaccagc
     4621 gccctgaccg gacccgcgct ctggccgcgc atggcgcgct gggacgcggg cgagatggac
     4681 gcggcgctcg ctgccgtgcg cacgccgcta cttgcgatcc agagcaccac gcgcgacgcc
     4741 cagctgcgcc gctcgccgct caaaccgggc gacacctccc cctggctcga gctgctgaag
     4801 aaatctatcc ccggcgcgaa gatcgagatc attccgggcg tcggccactt cccgcagctc
     4861 gaagccgcgg agaccgtcaa ccgcctgatc gccggattcg ttcagaagcc gtagagctcc
     4921 gcgggattgc cggcaagaat tctgttgcgc gtcgcttcgt caggtgccca ttgcaggtcg
     4981 agcagatggg cggcgtccgg catgtcgatg attttagggt cggcccctgc ttccggtaga
     5041 gtcgagatgt ggcgcggtgg cagtaggtcc ggtttgtgtg ttgccgcccc tttcgtctgg
     5101 cggtgcccga gtaacctcgc cgtcgctccg tttccacatc ccgctcatcg aaccggacgc
     5161 gcagatctcc cgcatccggc tttcggacaa gacctcacgc cttcgtccac gacacgtcgt
     5221 gcccaagcgc agtcagacgc acgagaccga agtacccgta gaggtgcgag agtggatagc
     5281 tcccgcccct gcgccgcctg accttgtgct tggtgcgtaa ccaccggcgt aaccgcgcag
     5341 ccgtgtagtt gtccagcgcc cggtatgcgc gactgacggt gcctac
//
LOCUS       DASPXW010000127         2155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_543607, whole genome shotgun sequence.
ACCESSION   DASPXW010000127 DASPXW010000000
VERSION     DASPXW010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543607"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VEL04_03040"
     CDS             complement(<1..195)
                     /locus_tag="VEL04_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HYT47372.1"
                     /translation="MTRATFDDVIDKLRERFEVEDNQKDDMPWSAEEFRRRIADKDGV
                     LPTGSDRVDAATLDAAPRLKA"
     gene            complement(363..>2155)
                     /locus_tag="VEL04_03045"
     CDS             complement(363..>2155)
                     /locus_tag="VEL04_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HYT47373.1"
                     /translation="RVLDLFVQRTYGVMSNDVLIAVPLFLFMGYLVERAQLIDRLFRS
                     LHLASARVPGSLAVATIVTCAIFATATGIVGAVVTLMGLLAFPAMLKAGYNVKVAAGA
                     VTAGGCLGILIPPSVLLIVYGSVAGVSVVQLYAGAFLPGFMLAGLYIGYIIVLAKWKP
                     KLMPPLPESERRVELPRWAQTLAPRGRNALAGLWRAAIGGNAGVAKRTVLAQLFVTLV
                     PALFIAALLGVTYRMATAPEVEASTAGLIEAGGLVAAPEQEREQSTGLIGAQEPEEKE
                     SGVKEPSAESSQAPAAPQAGAEAAKAPQTAAATAGKTGDAAESERLPVPLWLWITLGV
                     GIAIIVFVYWLWSWERLEVFKMLLTSFFPLALMILAVLGSIVFGLATPSEAAAIGGFG
                     GFILAGVYRFIAYWREGKSGGKDTGKAVWSTVKELGVIVKESSFLTAKTSAMVCWLFV
                     GSGIFSAAFALLGGQEVIEKWVLSLNMTPVQFMILAQFIIFILGWPLEWTEIIIIFMP
                     IFLPLLPHFHIDPLFFGLLVALNLQTAFLSPPVAMAAFYLKGVSPPHVTLNAIFAGMM
                     PFMGIQLIALVLLYLFPGIGLWLPQLVYGR"
BASE COUNT          431 a          722 c          676 g          326 t
ORIGIN      
        1 ggccttcaag cgcggtgcgg cgtcgagtgt cgccgcatcc acacggtcgg aaccggtggg
       61 aagcacgccg tccttgtcgg cgatccgccg ccgaaattcc tccgcgctcc aaggcatatc
      121 gtccttctgg ttgtcctcga cttcgaatcg ctcgcgcagc ttgtcgatga cgtcgtcgaa
      181 ggtcgcgcgg gtcacgagaa tcttgggttt cacgtcttct ccggaaagcg atctgccgca
      241 ggccggctac ggagaccggg tcgcacctac tgcgccggcg gcaatacgaa atataaaatg
      301 aatctccggc aaaaggaaag ggcggctcga agccgcccct tcggcagaga gtgatctcgg
      361 attcagcggc cgtagaccaa ttgcggcagc cacaggccga tgcccgggaa caggtacagc
      421 agaaccagcg cgatgagctg gatccccatg aacggcatca tccccgcgaa gatcgcgttg
      481 agggtcacgt gcggcgggga cacgcccttc aggtagaacg ccgccatcgc caccggcggc
      541 gacaggaacg cggtctgcag gttcaacgcc accagcaggc cgaagaacag cggatcgata
      601 tggaagtgcg gcagcagcgg caggaagatc ggcatgaaga tgatgatgat ctccgtccac
      661 tcgagaggcc agccgaggat gaagatgatg aactgcgcca ggatcatgaa ctgcaccggc
      721 gtcatgttga gcgacagcac ccatttctcg atgacctcct gcccgccgag cagcgcgaac
      781 gccgccgaga aaatccccga gccgacaaac agccagcaga ccatcgcgct cgtcttggcg
      841 gtgaggaagg acgactcctt gacgatgacg ccgagctcct tcaccgtgct ccagaccgcc
      901 ttgccggtgt ccttgccgcc ggacttcccc tcgcgccaat aagcgatgaa gcggtagacc
      961 cccgccagga tgaaaccgcc gaatccgccg attgccgccg cctcggaggg cgtcgccagc
     1021 ccgaatacga tcgagcccag cactgccagg atcatcagcg ccagcggaaa gaacgaggtg
     1081 agcagcatct tgaaaacttc cagccgctcc cagctccaca gccagtagac gaatacgatg
     1141 atggcgatgc cgacgcccag cgtgatccaa agccagaggg gcaccggcag cctttcggat
     1201 tccgctgcgt cgccagtttt cccggcggtt gccgccgcgg tctgcggggc tttcgcggcc
     1261 tccgcgcctg cctgaggagc ggcgggcgcc tgcgacgact cggctgaagg ttccttgacc
     1321 cccgattcct tttcttccgg ttcctgggcg ccgatgaggc ccgttgactg ctctctttcc
     1381 tgttcgggcg ccgcgacgag gcccccggcc tcgatcagcc cggccgtgct ggcctcgact
     1441 tccggcgcag tcgccatgcg ataggtgacg cccagcaatg cggcaatgaa gagcgccggc
     1501 accagcgtga cgaagagttg cgcgagcacg gtgcgttttg cgacaccggc gttgccgccg
     1561 atcgctgcgc gccagagccc ggccaacgcg tttctgccgc gcggcgccag cgtctgcgcc
     1621 cagcgcggaa gctccacgcg ccgctcgctc tcggggagcg gaggcatgag cttgggtttc
     1681 cactttgcga gcacgatgat gtaaccgata tagagccccg cgagcatgaa accgggcagg
     1741 aaggcgccgg cgtagagctg aacgaccgac acgcccgcaa cagagccata gacaatcagc
     1801 agcaccgagg gcgggatcag gatgccgagg cagccgcccg cggtcaccgc gcccgccgcc
     1861 accttgacgt tgtatcccgc cttgagcatc gccggaaagg cgagcagtcc catcaacgtc
     1921 accaccgcgc cgacgatgcc ggtggcggtc gcgaagatcg cgcaagtgac aatcgtcgct
     1981 accgcaagcg acccgggcac gcgcgcgctg gccagatgca ggctcctgaa caagcggtcg
     2041 atcaactgcg cccgctcgac gagatagccc atgaacagga acagcggcac cgcgatcagc
     2101 acgtcgttcg acatcacgcc gtaggtgcgc tgcacgaaga ggtcgagcac ccgcc
//
LOCUS       DASPXW010000128         2044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1550582, whole genome shotgun sequence.
ACCESSION   DASPXW010000128 DASPXW010000000
VERSION     DASPXW010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1550582"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /gene="bamC"
                     /locus_tag="VEL04_03050"
     CDS             complement(<1..671)
                     /gene="bamC"
                     /locus_tag="VEL04_03050"
                     /inference="COORDINATES: protein motif:HMM:NF018509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HYT47374.1"
                     /translation="MRRRVCAALGALLAGACANLTEDALQGKVDYKATTTLPSLEVPP
                     DLTSPARDNRYVVPETGKSTATYSGYQAERREQRPSASSAVLPEFDRIKIERAGTQRW
                     LVVQEPPEKLWPLVKDFWQENGFLIKVELPEAGVMETDWAENRANIPQGGVRDLLGRF
                     LDQAYSSSERDKYRTRLDRVEDGKGTEVYISHRGMQEVYTTGRDPSNNAPGATAWQAR
                     ASDPEL"
     gene            complement(700..1620)
                     /gene="dapA"
                     /locus_tag="VEL04_03055"
     CDS             complement(700..1620)
                     /gene="dapA"
                     /locus_tag="VEL04_03055"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HYT47375.1"
                     /translation="MITGSLVAMVTPMRSGREQDGALDLARFRQLIDWHVAEGTDGIM
                     VVGTTGESPTVDFDEHKELIRIAVEHSRGRIPIIAGTGGNSTAEAIELTVSAKKNGAT
                     ACLSVVPYYNKPTQEGMYQHFRKIAETVDLPMILYNVPPRTVADLQNDTVLRLAQVPG
                     IIGIKDATANIERGTDLVKRAPRNFSIYSGEDSTALALILLGGHGVISVTANVAPKLM
                     HQMCAAALVGDVKKARELNLKLLPLHQRLFVEANPIPVKWALAEMGMIEHGLRLPLVP
                     LSEKFHQTVREALHEAGIALPGLRVVEGRG"
     gene            complement(1634..>2044)
                     /locus_tag="VEL04_03060"
     CDS             complement(1634..>2044)
                     /locus_tag="VEL04_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HYT47376.1"
                     /translation="AGKGLEAVAVDREPQVIERVRFIRADLENGDPWPFEGQRFGAIV
                     VTNYLYRPLFGQLEASLAEGGVLIYETFMTGNEKFGKPSNPAFLLRPGELLDAFKALT
                     LVAFEQGIVGRPKKAAVQRLCAIRAPVGSAKIAA"
BASE COUNT          300 a          700 c          667 g          377 t
ORIGIN      
        1 agctccggat cggaggcgcg cgcctgccac gcggtcgcgc ccggcgcgtt gttcgacggg
       61 tcgcgccccg tggtgtagac ctcctgcatg ccgcggtggc tgatgtacac ctcggtgccc
      121 ttgccatcct ccacgcggtc gagccgcgtg cggtacttgt cgcgctcgga gctcgagtag
      181 gcctggtcga ggaacctgcc gagcaggtcg cgcaccccgc cctgcgggat gttggcgcgg
      241 ttctccgccc agtcggtctc catcacgccg gcctcgggca gctcgacctt gatgagaaag
      301 ccgttctcct gccagaagtc cttcaccagc ggccagagct tctccggcgg ctcctgcacc
      361 accagccagc gttgcgttcc ggcgcgctcg atcttgatgc ggtcgaactc gggcagcacc
      421 gccgaggaag ccgacggccg ctgctcgcgc cgctcggcct ggtagcccga atacgtcgcg
      481 gtcgattttc cggtttccgg aacgacgtag cgattgtcgc gtgccggcga ggtgaggtcc
      541 ggcggcacct cgagcgaggg cagcgtggtg gtcgccttgt agtcgacttt gccctgcagc
      601 gcgtcctcgg tgaggttggc gcacgcgccg gcgaggagcg cgccgagcgc ggcgcaaact
      661 ctccggcgca tcagccgcgg cctgggattg cgcgaggcat caaccgcggc cttccaccac
      721 gcgcagtccg ggaagcgcga tgcccgcctc gtgcagcgct tcgcgcaccg tctggtggaa
      781 tttttccgac agcggcacga gcggcaggcg caatccgtgc tcgatcatgc ccatctcggc
      841 gagcgcccac ttgaccggga tcgggtttgc ctcgacgaac aggcgctggt gcagcggcag
      901 gagcttgaga ttgagctcgc gcgccttttt cacgtcgccc accagcgccg cggcgcacat
      961 ctggtgcatc agcttcggcg ccacgttcgc ggtcaccgag atgacgccgt ggccgccgag
     1021 caagatgagc gccagcgcgg tggaatcctc gccgctgtag atcgagaaat tgcgcggcgc
     1081 gcgcttcacc agatcggtgc cacgctcgat gttggcggtc gcgtccttga tgccgatgat
     1141 gcccggcacc tgcgccaggc gcagcacggt gtcgttctgc aggtcggcga cggtgcgcgg
     1201 cggcacgttg tacaggatca tcggcagatc gaccgtctcg gcgatcttgc ggaagtgctg
     1261 gtacatgccc tcctgcgtcg gcttgttgta gtagggcacg accgacaggc aggccgtggc
     1321 gccgttcttt ttcgcggaga cggtcagttc gatcgcctcc gcggtggaat tgccgccggt
     1381 gccggcgatg atcgggatgc ggccgcgcga atgctccacc gcgatgcgga tcagctcctt
     1441 gtgctcgtcg aagtcaaccg tcggcgactc gcccgtggtg ccgaccacca tgatgccgtc
     1501 ggtgccttcg gcgacgtgcc agtcgatgag ttgtctgaag cgcgcgaggt cgagcgcgcc
     1561 gtcctgctct cgtcccgatc tcatcggcgt gaccatcgca accaggcttc ccgtaatcat
     1621 gcgcagctcc cgttcacgcc gcgattttag cgcttccgac cggcgcgcgt atcgcgcaca
     1681 gcctctggac cgcggctttt ttcggccgcc ccacgattcc ctgctcgaac gcgaccagcg
     1741 tcagcgcctt gaaggcatcg agcagctcgc ccgggcgcag caggaatgct gggttcgacg
     1801 gcttgccgaa cttttcgttc cccgtcatga aagtttcgta aatcagcacg ccgccctcag
     1861 cgagcgaagc ctcgagctgc ccgaacaacg gccggtacaa gtaattcgta acgacgatgg
     1921 cgccgaaacg ttgaccttcg aagggccagg gatcgccgtt ttcgaggtcc gctctgatga
     1981 acctgactct ctcgatgacc tgcggctcgc gatcgaccgc caccgcctcc agccccttcc
     2041 cggc
//
LOCUS       DASPXW010000129         2049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1049130, whole genome shotgun sequence.
ACCESSION   DASPXW010000129 DASPXW010000000
VERSION     DASPXW010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049130"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VEL04_03065"
     CDS             complement(<1..446)
                     /locus_tag="VEL04_03065"
                     /inference="COORDINATES: protein motif:HMM:NF019813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HYT47377.1"
                     /translation="MRRRSRAERGATGDQRREIQRELERAGAPLTPDEIAQRLRVHGS
                     ARREFDAGIAALERAGDVVQNRAGALLIAKRIALVAGRVEGHPDGHGFLIPDEGGAPV
                     FLAAAEMRQLMHGDRAAVRVGGRDQRGRPVGAVVEVLERANRRIVGR"
     gene            506..592
                     /locus_tag="VEL04_03070"
     tRNA            506..592
                     /locus_tag="VEL04_03070"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:540..542,aa:Leu,seq:cag)
     gene            complement(606..>2049)
                     /locus_tag="VEL04_03075"
     CDS             complement(606..>2049)
                     /locus_tag="VEL04_03075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HYT47378.1"
                     /translation="GRAAAQAAQRRRARRAGFMRRIAFLPLLAALAACTVGPDYRRPE
                     MPLPAGYGENAQAGAVALALDWWKLYRDPALDELVAAARKSNADVRLAAARVQEAEGA
                     LREARAAIFPDVTGSYGYSRQRVSTVASPPVPAGLPIVRPNHTLAASTSFEVDFWGRF
                     GRASEAARANLLGTQLARDVVDLTLAGAVAQAYFALRSLDSQIVVLDNTIRGRRDSLE
                     ITRARAQAGLAADLDVYQAQAALADALAQKRDAERNRALVAHQLAQLAGRLDLKLAAG
                     DVFALPLAPLPPPGLPSALLDRRPDIRQAEEALVAANAQIGIARAALFPTLSLTGQAG
                     VQSADLNNLLTSGAGIWTVGFGLALPLFDAGRRQARVDQAQARREQAVANYQRAVETG
                     FREVADALVNVQQTGEGEAELLERLQAARNALELSTLRYQAGYSFYLEVLDAQRTAND
                     AELAFVRNRQARLAFSVDLMKALGGGWKAD"
BASE COUNT          251 a          731 c          776 g          291 t
ORIGIN      
        1 cggccgacga tgcgccggtt agcccgctcg agcacctcga ccacggctcc gaccggccgg
       61 ccgcgttgat cgcggccgcc cacgcgcacc gcggcacggt cgccgtgcat cagctggcgc
      121 atttcggcgg cggcgaggaa caccggggcg ccgccttcgt cggggatgag gaagccgtgg
      181 ccgtcggggt gcccctcgac gcgccccgcg acgagcgcga tgcgctttgc gatcagcagc
      241 gctccggcgc ggttctgcac cacgtcgccg gcgcgctcca gcgcggcgat gccggcgtcg
      301 aactcgcgcc tggcgcttcc gtgcacgcgc aggcgctgcg cgatctcgtc gggcgtgagc
      361 ggtgcgccgg cgcgctcgag ctcgcgctgg atctcgcggc gctgatcgcc ggttgcgccg
      421 cgctcggcgc gtgagcgcct tctcaaggct gtccggctgg gtcttttttt aggcacttag
      481 ttttcggcta aaatcgcgcc tcgctgccga ggtggcggaa ttggtagacg cactaggttc
      541 aggtcctagc ggtggcaaca ctgtgggggt tcgagtccct tcctcggcac cagccaaccg
      601 cgatgttagt ccgctttcca gccccccccc agcgccttca tcaggtcgac gctgaacgcg
      661 agccgcgcct ggcggttgcg cacgaaggcg agttcggcgt cgttcgcggt gcgctgggca
      721 tcgagcacct cgaggtagaa cgagtagccc gcttggtagc gcagcgtgga gagctcgagc
      781 gcgttgcgcg cggcctggag gcgctcgagc agctcggcct cgccctcgcc ggtctgctgc
      841 acgttgacga gcgcatccgc cacctcgcga aagccggtct ccacggcgcg ctggtagttc
      901 gccaccgcct gctcgcgccg tgcctgcgcc tggtcgacgc gtgcctggcg gcgcccggcg
      961 tcgaagagcg gcagcgccag gccgaagcca acggtccaga tgcccgcgcc ggaggtgagc
     1021 aggttgttga ggtcggcgct ctgcacgccg gcctggccgg tgagcgagag cgtcgggaac
     1081 agcgccgcgc gcgcgatgcc gatctgcgcg ttggcggcga cgagcgcttc ctcggcctgg
     1141 cggatgtccg ggcggcgatc gagcagcgcc gagggcagcc ccggcggcgg cagcggcgcg
     1201 agcggcagcg cgaacacgtc tccggcggcg agcttcaggt cgagccggcc ggcgagctgg
     1261 gcgagctggt gggcaacgag cgcgcggttg cgctccgcgt cgcgcttctg cgccagcgcg
     1321 tcggcgagcg ccgcctgcgc ctggtagacg tcgagatcgg ccgcgaggcc ggcctgcgcg
     1381 cgcgcgcgcg tgatctcgag cgagtcgcgc cggccgcgga tggtgttgtc gaggacgacg
     1441 atctgactgt cgagggagcg cagggcgaag tacgcctgcg cgacggcgcc ggcgagcgtg
     1501 aggtcgacga cgtcgcgcgc gagctgcgtg ccgagcaggt tcgcccgcgc cgcctcgctc
     1561 gcccgcccga agcggcccca gaagtccacc tcgaagctgg tcgaggccgc gagcgtgtgg
     1621 ttcggccgca cgatcggcag cccggcgggc accggcggcg aggcgacggt gctgacgcgc
     1681 tggcgcgaat agccgtagct gccggtgacg tcgggaaaaa tcgccgcgcg cgcctcgcgc
     1741 agggcgcctt ccgcctcctg gacgcgcgcc gcggcgaggc gcacgtcggc gttgctcttg
     1801 cgcgccgcgg cgaccagctc gtcgagcgcc ggatcgcgat acagcttcca ccagtcgagc
     1861 gcgagcgcca cggccccggc ctgcgcgttt tcgccgtagc ccgccggcag cggcatttcc
     1921 ggccgccggt agtccgggcc gacggtgcag gcggcaagcg ccgccagcag cggcaggaag
     1981 gcgatccgcc tcatgaagcc tgcgcggcgt gctcttcggc gctgcgcggc ttgcgccgcc
     2041 gcgcgacca
//
LOCUS       DASPXW010000130         3784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1426751, whole genome shotgun sequence.
ACCESSION   DASPXW010000130 DASPXW010000000
VERSION     DASPXW010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1426751"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /gene="waaF"
                     /locus_tag="VEL04_03080"
     CDS             <1..597
                     /gene="waaF"
                     /locus_tag="VEL04_03080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HYT47379.1"
                     /translation="TYAKSSTPMRLHYARLAESPGIEPQQPLPATRLRADAGEIAAVA
                     RRFGIEGRYAVLCPGAEYGPAKRWPYFRPLAEKIKLPAVLLGSANDLESCRTIPGKNL
                     AGKTTLDEAIELIAGAALVVSNDSGLMHVAAALGRPLVALFGSSSPEHTPPQSPAARV
                     LWLRLECSPCFQRECPLGHFRCMKEISIEQVLAEIRSL"
     gene            616..1056
                     /locus_tag="VEL04_03085"
     CDS             616..1056
                     /locus_tag="VEL04_03085"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HYT47380.1"
                     /translation="MSNKIFPAAQDAENAFYEALERCDLEAMMAVWAEDEDILCVHPA
                     GPRLTGQDQVRESWARIFSGGPRARLQVSQQVAISGMMLAVHSVFENFSIEGAQGDAR
                     PAPIVATNVYLRTAAGWRMIVHHASPAPAQPGPAPRAAPPKFLH"
     gene            1053..2000
                     /locus_tag="VEL04_03090"
     CDS             1053..2000
                     /locus_tag="VEL04_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYT47381.1"
                     /translation="MTPYAAPWWLPGGHLQTLYASLRPPPRLALERHRWETPDGDFID
                     VDFAGEPQARRLMVLFHGLEGCSDSHYARAFAAAACAAGWRLALPHFRGFSGEPNRKP
                     RAYHSGDSDEVDWILKKMSAAETDLCAVGISLGGNALLKWLGERGEAAAAIVRRAAAV
                     SAPLDLPAAGGALDRGVNRLLYTRFFLATLKPKSLAKLERFPGLYDAAKLRAATRFRE
                     FDDLVTAPLHGFRDVAHYWSTASSGPWLERVRLPTLVINARNDPFLPERAMLAAARKA
                     APCVLLEFPRGGDHAGFPVGPFPGRHTWLPQRLLEFFAA"
     gene            2003..3370
                     /locus_tag="VEL04_03095"
     CDS             2003..3370
                     /locus_tag="VEL04_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HYT47382.1"
                     /translation="MPAEIFRAYDIRGVVGRSLTAAIVRDIGRALGSLGRERGAPSFA
                     VCRDGRLSGPELVAALSEGLAAVGANVIDLGMAPTPIAYFAAQHLGCGSCVAVSGSHN
                     PPEYNGLKMVVAGDTLWGEEVQELRRRIERQQFKPASGKRSTADVLDAYVERIASDVK
                     LARRFHIAVDCGNGVAGMLAPRLYRRLGCEVTELYCEVDGRFPNHHPDPSQPKNLQEL
                     IQKLETTSCEIGLAFDGDGDRLGVVTKDGEIIYPDRLLMLFARDVLSRNPGAQIIYDV
                     KSTRLLAPWIERYGGKPVIWKTGHSLIKAKLKESGALLAGEMSGHTFFKERWYGFDDA
                     LYAGARLLEVLSKESNANQALKSLPNAPSTPELHWALEEGEPHALVARLQAVKPFPGA
                     ERVLTLDGVRVEYADGFGLARASNTTPVIVLRFEADTAAALERIKQAFRSALQPLKPN
                     APLPY"
     gene            3432..>3784
                     /locus_tag="VEL04_03100"
     CDS             3432..>3784
                     /locus_tag="VEL04_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HYT47383.1"
                     /translation="MRSIDERERSLCEYAGKVLLVVNTASQCGYTPQYEGLEALYRKY
                     RARGLVVLGFPSNDFGGQEPGSNQEIASFCVNQYAVDFPVFAKTELKKNSLYADLAKA
                     SGQAPRWNFHKYLVDR"
BASE COUNT          620 a         1300 c         1255 g          609 t
ORIGIN      
        1 acgtatgcga agtcaagcac cccgatgcgg ctgcattacg cccggcttgc cgaatccccc
       61 ggaatcgaac cgcagcaacc tttgcccgca acgcgtctgc gggcggatgc cggcgaaatc
      121 gccgccgtgg cccggcgctt cggcatcgag gggcgctacg cggtgctttg ccccggcgcg
      181 gaatacgggc ccgccaagcg ctggccttat ttccggccgc ttgccgagaa aatcaagctt
      241 ccggcagtgc tgctcggatc ggcgaatgat cttgagagtt gcaggacaat tcccggaaaa
      301 aatctcgccg gcaagaccac cctcgatgag gcgatcgagc tcatcgccgg cgccgcgctc
      361 gtggtgagca acgattcggg cctgatgcac gtcgcggcgg cgctcggccg gccgctggtg
      421 gcgctgttcg gctcctcgag cccggaacac acgccgccgc aatcgccggc ggcgcgcgtg
      481 ctgtggctgc ggctcgagtg cagcccgtgc tttcagcgcg aatgcccgct cgggcacttc
      541 cgttgcatga aagaaatctc gatcgagcag gtgttagcgg aaatccgcag cctttgagcg
      601 cgaaaatctt ccaaggtgag taacaagatc ttccctgcag cgcaggatgc agagaacgcc
      661 ttctacgagg cgctcgagcg ctgcgacctc gaagccatga tggccgtgtg ggccgaggac
      721 gaggacatcc tgtgcgtgca ccccgccggg ccgcggctga ccggccagga ccaggtgcgc
      781 gagagctggg ccaggatctt ctccggcggg ccgcgcgcgc ggctgcaggt gagccagcag
      841 gtcgcgatca gcggcatgat gctcgcggtg cacagcgtgt tcgagaattt ttccatcgaa
      901 ggcgcgcagg gcgacgcgcg gccggcgccg atcgtcgcga ccaacgtgta cctgcgcacc
      961 gcggcgggct ggcgcatgat cgtgcatcac gcctcgcccg caccggcgca acccgggccc
     1021 gcgccgcgtg cggcgccgcc caagttcctg cattgactcc ttacgccgca ccctggtggc
     1081 tgccgggcgg ccacctgcag accctgtacg cgtcgcttcg cccgccgccg cggctcgcgc
     1141 tcgagcgcca tcgctgggaa acgcccgacg gcgacttcat cgacgtcgac ttcgccggcg
     1201 agccgcaggc gcgccgcctg atggtgctgt tccacggcct cgagggctgc tcggacagcc
     1261 actacgcgcg cgcgttcgcg gccgccgcat gcgcggccgg ctggcggctc gcgctgccgc
     1321 attttcgcgg cttctcgggc gagcccaacc gcaagccgcg cgcttatcac tccggtgata
     1381 gcgacgaagt cgattggatc ttgaaaaaaa tgagcgccgc agagacggac ctatgcgcgg
     1441 ttggcatctc cctgggcggc aacgcgctgc tcaaatggct gggggagcgc ggcgaggctg
     1501 cggccgcgat cgtgcggcgc gcggccgctg tctcggcacc actcgacctg ccggccgcgg
     1561 gcggcgcgct ggaccgcggt gtcaatcgcc tgctatacac ccggttcttc cttgccacgc
     1621 tgaagccgaa atcgcttgcc aagctggaac gcttcccggg tctgtacgat gcagcgaagc
     1681 tgcgcgcggc gacgcgcttc cgcgagttcg acgacctcgt caccgcgcca ctgcacggct
     1741 ttcgcgacgt ggcgcactac tggtccacgg cttcgagcgg gccctggctc gagcgcgtgc
     1801 gcctgccgac cctggtgatc aacgcgcgca acgacccctt ccttccggag cgggccatgc
     1861 tcgccgcggc gcgcaaggcc gcgccttgcg tgctgctgga atttccgcgc ggcggcgacc
     1921 acgccggatt tccggtcgga ccgttcccgg ggcggcacac ctggctgcct cagcgcctgc
     1981 tggaattctt cgccgcttga gcctgcctgc cgaaatcttt cgcgcctacg acatccgcgg
     2041 cgtggtcggc cgcagcctca cggccgcgat cgtgcgcgat atcggccgcg cgctcggctc
     2101 gctcgggcgc gagcgcggcg cgccgagctt cgcggtttgc cgcgacggca ggctgtcggg
     2161 gccggagctg gtcgctgccc tgtccgaggg gctcgcggcg gtgggcgcca acgtcatcga
     2221 cctcggcatg gcgccgacgc cgatcgccta cttcgccgcg cagcacctgg gctgcggcag
     2281 ctgcgtcgcg gtgagcggca gccacaatcc gcccgaatac aacggcctca agatggtggt
     2341 ggcgggcgac accctctggg gcgaggaggt gcaggagttg cgccgcagaa ttgaaagaca
     2401 gcaattcaag cccgcctcag gaaaacgcag caccgcggat gtgctggacg cctacgtcga
     2461 gcgcatcgcc tccgacgtga agctggcgcg gcggttccat atcgcggtcg actgcggcaa
     2521 cggcgtcgcc ggcatgctcg cgccgcggct ctaccggcgc ctgggctgcg aggtgaccga
     2581 gctctactgc gaggtcgacg ggcgattccc gaaccatcat cccgatcctt cgcaacccaa
     2641 aaatcttcag gaattaattc aaaagctcga aaccacaagc tgtgagatcg gcctcgcctt
     2701 cgacggcgat ggcgaccggc tgggggtggt gacgaaggac ggcgagatca tctaccccga
     2761 ccgcctgctg atgctgttcg caagagacgt gctctcccgc aacccgggcg cccagatcat
     2821 ctacgacgtg aagagcacgc ggctgctcgc gccgtggatc gagcgctacg gcggtaaacc
     2881 cgtgatctgg aagaccggcc actcgctgat caaggcgaag ctcaaggaat cgggggcgct
     2941 gctcgcaggc gagatgtcgg ggcatacctt cttcaaggag cgctggtacg ggttcgacga
     3001 cgcgctctac gcaggagccc gccttttgga ggtcttgtca aaagaaagca atgccaacca
     3061 ggcgctcaag agcctgccca acgccccttc cacccccgag ctgcactggg ccctcgagga
     3121 gggcgagccg catgcgctcg tcgcccggct gcaggcagtg aaacccttcc ccggggccga
     3181 gcgcgtgctg accctcgacg gggtgcgggt cgagtacgcc gacggcttcg gcctggcgcg
     3241 cgcttccaac accacgccgg tgatcgtgct acgcttcgag gccgatacgg ctgccgcgct
     3301 ggagcgcatc aaacaggcgt tccgcagcgc gttgcagccg ctgaaaccga atgcgcccct
     3361 gccttattag ctttctgttg ctgatcaaca gcgcagcgct cgccgcgtgc ccgccgctgc
     3421 tcgaccgcag catgcgctcg atcgacgagc gcgagcggtc gttgtgcgag tacgccggaa
     3481 aggtgctgct cgtcgtcaac accgcgagcc agtgcggcta tacgccgcag tacgaggggc
     3541 tcgaagcact ctaccgcaag taccgcgcgc gcgggctggt ggtgctcggc tttccgtcaa
     3601 acgatttcgg cggccaggag cccggctcga accaggagat cgccagcttc tgcgtcaatc
     3661 agtacgccgt cgactttccg gtgttcgcaa aaacagaatt gaaaaagaac tccctttacg
     3721 cggacctggc gaaagccagc ggccaggcgc cgcgctggaa cttccacaag tacctggtcg
     3781 atcg
//
LOCUS       DASPXW010000131         2503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1303631, whole genome shotgun sequence.
ACCESSION   DASPXW010000131 DASPXW010000000
VERSION     DASPXW010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1303631"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VEL04_03105"
     CDS             <1..477
                     /locus_tag="VEL04_03105"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HYT47384.1"
                     /translation="RRFGWKAYYGDATRLDLLESAGAAAARVLVLAIDDPRAALTSVK
                     RVRERFPQLAIVARARSRTEAYEFAELGVPAVREVFSSALDTAVRTLVILGFGEKDAQ
                     RIAQRFRQHDEELTAKNAPHRHDIGKLIALSEQGRSDLQQLLAAETALAPQIDRND"
     gene            539..871
                     /locus_tag="VEL04_03110"
     CDS             539..871
                     /locus_tag="VEL04_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47385.1"
                     /translation="MRKPKGSGKLQREEADPPPDVAEPLQDRVIERPDGFYWESKAKR
                     ETHGPFATLAEAEADMLSGGAAAAEDVELGESLHEAESELGISEWIDPDTGVPAEDSV
                     PRIEDQDH"
     gene            complement(876..1922)
                     /locus_tag="VEL04_03115"
     CDS             complement(876..1922)
                     /locus_tag="VEL04_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47386.1"
                     /translation="MWLVRIAAEDAVFHAPSLSMKLRTPAFCRPVRKRAYFVREMNRT
                     SKFWVLGLALAGALAAAGCHAQNTPATPTEAQARDYVYGAFLTGAAADILSERVNLGP
                     ELQQRLAVSPRADYHRIYDALIALTEYQPLRVRKPTPDEVADYTSRVKRDYQGPLFTV
                     EAGDIRLLVQYDLPANNISFVGQLSPTPERPTAAAVERAVVPPPPPAPAAAPKPPPIA
                     IEKPAPPPVAIQKPQKPTPPVAAVESPKPRVVAPERPNPAARVEPPERTGPCEIKSVM
                     SDQDLINCSAPAATMEKPRPQTPAMERATPAVVAVEKPKPVTRAEERKPAAPCVVKPV
                     MSEEDLLNCGARPR"
     gene            complement(1938..>2503)
                     /locus_tag="VEL04_03120"
     CDS             complement(1938..>2503)
                     /locus_tag="VEL04_03120"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HYT47387.1"
                     /translation="AYIVTEYFPAGCLRQAVKGPLPPRQALSLLAQAATALAEVHRQG
                     IVHRDLTPENLMVRENGEVALAGFENAKDETNGTGMTQRSAPLRSLYYRSPEHVTGKP
                     VDRRSDLYALGVIFFEMLTGHRPYTSDSIDELTRQIVTAPPPRLPAEIAHCQELVDAM
                     LAKDPARRCPSAGAVLDAIDTVRTRAE"
BASE COUNT          405 a          825 c          835 g          438 t
ORIGIN      
        1 cggcgcttcg gctggaaagc ctattacggc gatgcgacgc gcctcgacct gctcgagtcg
       61 gcgggcgcgg cagccgccag agtgctggtg cttgccatcg acgacccgcg cgccgcgctc
      121 acctcggtga agcgcgtgcg cgagcgcttc ccgcagctcg ccatcgtcgc ccgcgcccgc
      181 agccgcaccg aggcttacga atttgccgag ctgggcgtgc ccgcggtgcg cgaagtgttc
      241 agctcggcgc tcgacaccgc agtgcgaacc ctggtcatcc tgggattcgg ggaaaaagac
      301 gcgcagcgca tcgcgcagcg cttcaggcag cacgacgagg agctcaccgc gaaaaacgcg
      361 cctcaccgcc acgacatcgg caagctcatc gcgctctccg agcaaggccg cagcgacctg
      421 cagcaactgc tggccgcgga aaccgcgcta gcgccgcaga tcgaccggaa cgactagtgc
      481 tgtcgagcgt ttcccgctct tgcgccacgc cgcggatttt ggcgtaggat ggaacatcat
      541 gcgcaagccg aaaggcagtg ggaaattaca gcgcgaggaa gcggatccgc ctccggacgt
      601 agcggaaccg ctgcaagacc gcgtcatcga gcggcctgac ggcttttact gggaatccaa
      661 ggcgaagcgc gagacccacg gtcccttcgc caccctcgct gaggccgaag ccgacatgct
      721 ttccggcggc gccgctgccg cggaagacgt cgagctcggc gaatcgctgc acgaagcgga
      781 atccgagctc ggcatctcgg agtggatcga tccggatacc ggcgtgcctg ccgaggactc
      841 ggtcccccgc atcgaggacc aggaccactg attccctagc gcggccttgc gccgcagttg
      901 agcaggtcct cttccgacat caccggcttg accacgcacg gggccgcggg cttgcgttct
      961 tcggctctcg taacgggctt cggcttctcc accgcaacga cggccggcgt tgctctttcc
     1021 attgcggggg tctgcggcct cggcttttcc attgtggcag ccggcgcgct gcagttgatg
     1081 aggtcctggt ctgacatcac cgacttaatc tcgcaggggc cggttcgctc tggcggttcg
     1141 accctagctg cgggattcgg cctttccggt gcgacaacgc ggggcttcgg gctttccacg
     1201 gcagcgaccg gtggcgtcgg cttttgcggc ttttgaattg ccaccggcgg cggcgcaggc
     1261 ttctcgatag cgatcggcgg tggctttggc gctgccgctg gcgcaggcgg cggtggcggc
     1321 acgaccgccc gttcaaccgc cgcggcggtc ggacgttccg gcgttgggct cagctggccg
     1381 acgaaggaaa tgttgttggc cggcaggtcg tactgaacga ggagcctgat gtcgcccgct
     1441 tcgaccgtga acagcggccc ctggtagtcg cgcttcacgc gcgaggtgta atcggcaacc
     1501 tcgtccggcg tgggtttgcg cacgcgcaag ggctggtatt cggtgagcgc gatcaacgcg
     1561 tcgtagatcc tgtggtagtc cgcgcgcggc gagacggcga ggcgctgctg cagttccggc
     1621 ccgagattca cgcgctcgct gagaatatcc gccgcagcgc cggtcaggaa agcgccatac
     1681 acgtagtcgc gcgcctgcgc ttcagtgggc gtagcgggcg tgttctgggc atggcaaccc
     1741 gctgcggcca atgcgccggc cagcgccaat cccagcaccc agaatttcga ggttctgttc
     1801 atctcccgta caaagtatgc tcttttgcgc accggcctgc agaaggcggg ggtccgcagt
     1861 ttcatcgata aactcggggc atggaatacc gcgtcctcgg cggcgatgcg gaccagccac
     1921 acgcataccc ccctggttca ttcggcgcgc gtccgaacgg tatcgattgc gtcgagcacc
     1981 gcaccggcgc tcgggcagcg ccgcgccgga tccttggcca gcatggcgtc gaccagctcc
     2041 tggcaatggg cgatttcagc gggtaaccgc ggaggcggcg cggtgacgat ctgccgggtc
     2101 agctcgtcga tggagtcgct ggtgtagggc cgatggcccg tcagcatttc gaaaaaaatc
     2161 acgccgaggg cataaagatc cgagcgccga tcgaccggct tgcccgtcac atgttccggc
     2221 gaccggtaat aaagcgaccg cagaggcgcg ctgcgctgtg tcatgcccgt gccgttcgtc
     2281 tcgtccttgg cgttctcgaa gccggcgagc gcgacttcgc cgttttcgcg caccattaaa
     2341 ttttccggtg tcaggtcgcg gtgaacgatt ccctggcgat gcacctccgc cagcgcggtt
     2401 gcggcctgcg cgagcagcga cagcgcctgc cgcggtggca gcggaccctt gaccgcctgt
     2461 ctcaggcagc cggccggaaa atactccgtg acgatgtagg cga
//
LOCUS       DASPXW010000132         9678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_418217, whole genome shotgun sequence.
ACCESSION   DASPXW010000132 DASPXW010000000
VERSION     DASPXW010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9678
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418217"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..1415
                     /locus_tag="VEL04_03125"
     CDS             42..1415
                     /locus_tag="VEL04_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HYT47388.1"
                     /translation="MKRAEQRGAARGAQVLVVDDEPDIRELLELTLVKMGLGVQSVGT
                     LAEAKSKLAEERFDLCLTDMRLADGEGLDLVRHISGLGADLPVAVITAYGSAENAVAA
                     LKAGAFDYVSKPVGLEQLRALIRSALSLPDRAETPPGGRRLLGESQPLTQVRAMISKL
                     ARTQAPVYISGESGSGKELAARLIHEHGARRDKPFVPVNCGAIPETLMESEFFGYKKG
                     AFTGAAEDREGFFQAANGGTLFLDEVADLPLAMQVKLLRAIQERRVRKVGSTQEDPVD
                     VRIICATHQKLAALVAAGRFRQDLFYRVNVIELTMPPLRECREDVGILAASILERLAP
                     QGAGPAPRLTPAALDELSRYDFPGNIRELENILERALALSGAQEIGIEDLRLAPSAAE
                     GGSELGEPGGSLPEYLDSVERKAIHEALAKTEFNRTAAAKLLGITFRQLRYRMQRLNI
                     KDDNKSL"
     gene            1424..1966
                     /gene="ampD"
                     /locus_tag="VEL04_03130"
     CDS             1424..1966
                     /gene="ampD"
                     /locus_tag="VEL04_03130"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017423641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HYT47389.1"
                     /translation="MKIGPEGLLAGARYVASPNCDERPPGCEITLLVVHSISLPPGEY
                     GGDGIERLFTNRLEPQAHPYFGEIFSLKVSAHFLVRRNGEVVQFVPVQQRAWHSGVSS
                     WRGRGACNDFSIGVELEGTEESAFEDAQYQSLSVLIRSLRSSLPLKDIAAHGDVAPGR
                     KTDPGAHFDWARVLADLVRS"
     gene            2212..5046
                     /locus_tag="VEL04_03135"
     CDS             2212..5046
                     /locus_tag="VEL04_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HYT47390.1"
                     /translation="MQIAQDSATATAALGVGLPESSPSAHNLFADFKVIRRNGAVVGF
                     EPAKISVAVTKAFLAVNGSQGAASARIRELVSGLTEAVVGALLRRQPQGGTFHIEDIQ
                     DQVELALMRSGEHDVARAYVLYREERAKLRANEKAARATDAGIHVIENGQRKPLEREA
                     LGRMVAAACEGLAGALPEAILETTLRDLYDGVPMDEVRKSAVLAARALIEKDPAYSYV
                     TARLLLNIIRLEVLGEEASQADMATRYAAYFPQFIERGIAAELLDPELGRFDLTRLGG
                     ALKAERDPQFGYLGLQTLYDRYFLHHHGRRIELPQAFFMRVAMGLAIKEGAPGEREAR
                     AIEFYNVLSTFDFMSSTPTLFNSGTLRSQLSSCYLTTVSDDLEGIYEAIKENALLAKY
                     AGGLGNDWTPVRALGSHIKGTNGKSQGVVPFLKVVNDTAVAVNQGGKRKGAVCSYLET
                     WHLDVEEFLELRKNTGDDRRRTHDMNTANWIPDLFMKRVMDNAEWTLFSPADVRDLHD
                     KTGKAFEEAYLRYEDRAARGELKLYKKIPAMQLWRKMLSMLFETGHPWITFKDPCNLR
                     SPQQHTGVVHSSNLCTEITLNTSADEIAVCNLGSVNLLAHMTETGLDFRKLKNTIRTA
                     MRMLDNVIDINYYAVAKARNSNLRHRPVGLGIMGFQDCLHRLRIAYASSEAVEFADRS
                     MEMVAYCAYWASTELAEERGRYASFQGSLWDRGVLPQDTLDLLAAERGGYVELDRSAT
                     LDWEALRARIKRHGMRNSNCLAIAPTATIANIVGVSASIEPTYQNLFVKSNLSGEFTV
                     VNEYLVVDLKQAGLWDEVMIADLKYFDGNLARIDRVPAALRRLYATAFEVEPKWLVEA
                     AARRQKWIDQSQSLNIYMAGASGKKLDETYKLAWIRGLKTTYYLRSMGATHAEKSTSK
                     AGQLNAVSSGGEAEEAKVCAIDNSECEACQ"
     gene            5043..6164
                     /locus_tag="VEL04_03140"
     CDS             5043..6164
                     /locus_tag="VEL04_03140"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HYT47391.1"
                     /translation="MKLENLALQPPGGFVPATGASAGAAAERFQVGNACRRVTVEDKS
                     VINSKADVNQLVPFKYKWAWEKYLAACANHWMPQEINMSRDIALWKDPAGLTEDERRV
                     IKRNLGFFVTADSLAANNIVLGTYRHITAPECRQYLLRQAFEEAIHTHAYQYIVESLD
                     LDESEIFNAYHEIHSIRDKDEFLVPFIDTLTDPHFTTGTPANDQALLKSLIVFSCLME
                     GLFFYVGFTQILSLGRQNKMTGAAEQYMYILRDESMHCNFGIDLVNQIKLENPQLWTA
                     SFREELADIFRRAVELEYRYAEDTMPRGVLGLNAPMFKEYLRFICNRRCQQIGLETLY
                     PGASNPFPWMSEMIDLKKERNFFETRVIEYQTGGALSWE"
     gene            6188..6490
                     /locus_tag="VEL04_03145"
     CDS             6188..6490
                     /locus_tag="VEL04_03145"
                     /inference="COORDINATES: protein motif:HMM:NF021873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HYT47392.1"
                     /translation="MEEEWDDMKITACKAEDDYKLWLRFEDGLEGSVFLGNLLEIGAF
                     QLWRDVREFEKVSVDPETATVTWEGGIRLDADILYYDLKNNPIQHAAAASPPSVVE"
     gene            6623..6847
                     /locus_tag="VEL04_03150"
                     /pseudo
     CDS             6623..6847
                     /locus_tag="VEL04_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814930.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histone"
     gene            7313..7996
                     /locus_tag="VEL04_03155"
     CDS             7313..7996
                     /locus_tag="VEL04_03155"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HYT47393.1"
                     /translation="MTTTLVAARAYAALKGGLALVGLAALAAFFVLPTRPDAVLPQLF
                     SLAGDAFGENPTVAAGPIQARMETPLEREQRAMTAYIAKRYRVADTAVAGFVTAAYRS
                     GEQYSVDPLLILAVMAVESRYNPVAESTVGAKGLMQVIPKFHLEKLMDHGGEQALLEP
                     EVNIQVGARILREYQRRFGDTETALQMYAGAFDEPTAQYASKVFAEKARFESLRQLLR
                     QKARKQQSV"
     gene            complement(7962..9140)
                     /locus_tag="VEL04_03160"
     CDS             complement(7962..9140)
                     /locus_tag="VEL04_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007589502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYT47394.1"
                     /translation="MPTPVANLAARRVAAIEPFEVMDVLARAHALEAAGRKVIHMEIG
                     EPDFTAPAPVVEAGVRALRDGLTAYTATLGLPQLRAAIAAHYEARFSKNIPADSIAIT
                     SGASGGLLTVTALYVDPGDEILLPDPGYPGYRHFVRAFEGVARTLPVSAAQNFQPTLD
                     MVRSAWSARTKGLLLGSPSNPTGTLISKTELEKIAHFVAERGSVLLVDEIYQGLVYGI
                     EPWSALGLPGEVVVINSFSKYFCMTGWRLGWVVLPEGAVRGFEKLAQHLFISAPSAAQ
                     HAACSAFSPQSLKILEGRRSEFEKRRDFLVPALRQAGLAVPAEPHGAFYVYADCGGDA
                     RRFCLELLEKEAVAATPGIDFGANATARFVRFAYTRGLAELEEAAARVTRFAASWLSA
                     "
     gene            9166..>9678
                     /locus_tag="VEL04_03165"
     CDS             9166..>9678
                     /locus_tag="VEL04_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HYT47395.1"
                     /translation="MDEQLKNNALEYHRRSPPGKISILPTKQLTNQRDLALAYSPGVA
                     AACEEIVRDAAQAVRYTARANLIAVITNGTAVLGLGAIGPLAAKPVMEGKSVLFKKFA
                     GIDCFDIELDERDPQRLVDIIAALEPTFGAINLEDIKAPECFYIEETLRRTMKIPVFH
                     DDQHGTAIISG"
BASE COUNT         1851 a         3158 c         3160 g         1509 t
ORIGIN      
        1 gagggaggcg cgcgcggcat gaaccccgcc gggcagcccc gatgaagcgc gccgagcagc
       61 gcggcgccgc gcgcggcgcc caggtcctgg tcgtcgacga cgagcccgac atccgcgagc
      121 tgctcgagct caccctcgtc aagatggggc tcggcgtgca gagcgtgggc actcttgccg
      181 aagcgaaatc gaagctggcg gaggaaaggt tcgacctttg cctgaccgac atgcgccttg
      241 ccgacggcga ggggctcgac ctggtgcgcc atatctccgg cctcggcgcc gacctgccgg
      301 tcgccgtgat caccgcctac ggcagcgcgg agaacgcggt cgccgcgctc aaggcaggcg
      361 ccttcgatta cgtctccaag ccggtcggcc tcgagcagct gcgtgcgctc atcaggtcgg
      421 cgctgtcgct gccggaccgc gccgagacgc cgccgggagg ccggcggctg ctcggcgaaa
      481 gccagccgct cacgcaggtg cgcgccatga tctccaagct cgctcgcacc caggcgccgg
      541 tgtacatctc cggggagtcg ggcagcggca aggagctcgc cgcgcgcctc atccacgagc
      601 acggcgcgcg ccgcgacaag ccgttcgtac cggtgaattg cggcgccatc ccggaaaccc
      661 tgatggaaag cgagttcttc ggctacaaga aaggcgcgtt caccggcgcc gccgaagacc
      721 gcgagggctt cttccaggcg gcaaacgggg gcacgctgtt cctcgacgag gtggccgacc
      781 tgccgctcgc catgcaggtg aagctgctgc gcgcgatcca ggagcggcgc gtgcgcaagg
      841 tcggctcgac gcaggaggac ccggtcgacg tgcgcatcat ctgcgccacg catcagaagc
      901 tcgccgcgct ggtcgcggcg ggccgcttca ggcaggacct gttctatcgc gtcaacgtca
      961 tcgagctgac gatgccgccg ctgcgcgaat gccgcgagga cgtggggatt ctcgcggcga
     1021 gcatcctcga gcgcctcgct ccccagggcg cagggcccgc gccgcggctg acgcccgcgg
     1081 cgctcgacga gctctcgcgc tacgactttc ccggcaatat ccgcgagctc gagaacatcc
     1141 tcgagcgcgc gctcgccctg tccggggccc aggagatcgg catcgaggat ctgcggcttg
     1201 cgccgagcgc cgcggagggc gggtcggagc ttggcgaacc gggcgggtcg ctccctgagt
     1261 acctcgacag cgttgagcgc aaggcgatcc acgaggcgct cgccaagacc gagttcaacc
     1321 gcaccgccgc cgcgaagctg ctgggaatca ccttccgcca gcttcgctac cgcatgcagc
     1381 ggctcaacat aaaagatgac aataaatccc tctgaacggc gggatgaaaa tcggtcctga
     1441 aggcctgctc gccggcgccc gctacgtcgc ctcgcccaac tgcgacgagc gcccgccggg
     1501 ctgcgaaatc accctgctgg tggtgcactc gatcagcctg ccgccgggcg aatacggcgg
     1561 cgatggcatc gagcggctgt tcaccaaccg cctcgaaccg caagcgcacc cgtatttcgg
     1621 cgagatcttc tcgctgaagg tgtccgctca cttcctggtg cgacgcaatg gcgaggtggt
     1681 ccagttcgtg ccggtgcagc agcgcgcctg gcactcgggc gtctcgtcgt ggcgcgggcg
     1741 cggcgcgtgc aacgactttt ccatcggcgt cgagctcgaa ggcaccgaag agtcggcctt
     1801 cgaagacgcg cagtaccagt cactttcagt tctcataaga agcctgcgtt cctcgcttcc
     1861 cttgaaagac atcgccgcgc acggcgacgt ggcgccgggc cgcaagaccg accccggcgc
     1921 gcacttcgac tgggcgcgcg ttctcgccga cctggtccgc tcgtagctcc ccgactagat
     1981 atggtgcacg gcgtgtgccg atgcgctata tctagtagtg atgttgcacc gcgcaacgct
     2041 taaagtgctt gcaaaatgaa aatcgcgtgt gtacattgcg cccacaagat gtagctttga
     2101 atgaaggggt aagcactgaa tattgtggtc gtaggtgctg gtcgtcatac ggcggtaacc
     2161 acctcgggat tgcggccccg ataagcagtc aaggcctcgg gagggcgaga tatgcagatt
     2221 gctcaggata gcgccacggc gacggctgca ttgggcgtag ggctgcctga gtcgagcccc
     2281 tcggcgcaca acctgttcgc cgatttcaag gtgatccgcc gcaacggcgc ggtggtcggt
     2341 ttcgagcccg ccaagatctc ggtggccgtg accaaggcct tcctggcggt gaacggcagc
     2401 cagggcgccg cctcggcgcg catccgcgag ctggtctcgg ggctgaccga agcggtggtg
     2461 ggcgcgttgc tgcgccgcca gccgcagggc ggcaccttcc atatcgagga cattcaggac
     2521 caggtcgagc tcgcgctgat gcgctcgggc gagcacgacg tggcgcgcgc gtatgtgctg
     2581 taccgcgagg aacgcgcgaa gctgcgcgcc aatgaaaaag cggccagggc aaccgacgct
     2641 ggcattcatg tcatcgaaaa cggccagcgc aagccgctcg agcgcgaggc gctcgggcgg
     2701 atggtcgccg ctgcctgcga ggggcttgct ggcgccttgc ccgaggcgat cctggaaacc
     2761 accctgcgcg acctgtacga cggcgtgccg atggacgagg tgcgcaaatc agcggtgctc
     2821 gcggcgcgcg ccctgatcga gaaggacccg gcctacagct acgtcacggc acggctgctg
     2881 ctcaacatca tccgcctcga ggtgctcggc gaggaagcga gccaggccga catggcgacg
     2941 cgctacgccg cctactttcc gcagttcatc gaacgcggca tcgcggccga gctgctcgat
     3001 cccgagctgg gtcgctttga tctgaccaga ttgggcggcg ccctgaaggc cgagcgcgac
     3061 ccgcaattcg gctacctcgg gctgcagacg ctgtacgacc gctatttcct gcaccaccac
     3121 ggccggcgca tcgagctgcc gcaggccttc ttcatgcgcg tggccatggg cctcgccatc
     3181 aaggaaggag caccggggga gcgcgaggcg cgggcgatcg agttctacaa cgtgctctcg
     3241 acgttcgatt tcatgagctc gacgccgacg ctgttcaact ccggtacgct gcgctcgcag
     3301 ctttccagct gctacctgac gaccgtgtcc gacgacctcg agggcatcta cgaggcgatc
     3361 aaggaaaacg cgctgctcgc caagtacgcc ggcggcctcg gcaacgactg gacgccggtg
     3421 cgggcgctcg gctcccacat caagggcacc aacggcaagt cgcagggggt ggtgccgttc
     3481 ctcaaggtgg tgaacgacac cgcggtcgcc gtcaaccagg gcggcaagcg gaagggtgcg
     3541 gtgtgctcgt acctcgagac ctggcacctc gacgtcgagg agttcctcga gctgcgcaag
     3601 aacaccggcg acgaccgccg ccgcacgcac gacatgaaca cggcgaactg gatcccggac
     3661 ctgttcatga agcgcgtgat ggacaacgcg gagtggacgc tgttcagccc ggcggacgtg
     3721 cgcgacctgc acgacaagac cggcaaggcc ttcgaggaag cctacctgcg ctacgaggac
     3781 agggcggcgc gcggcgagct caagctctac aagaagatcc cggcgatgca gctgtggcgc
     3841 aagatgctgt cgatgctctt cgagaccggg catccgtgga tcaccttcaa ggacccgtgc
     3901 aatctcagga gcccgcagca gcacacgggc gtggtgcaca gctcgaacct gtgcaccgag
     3961 atcacgctca acacctcggc cgacgaaatc gcggtgtgca acctgggctc ggtcaatctg
     4021 ctcgcgcaca tgaccgagac gggactcgac ttccgcaagc tgaagaacac catccgcacg
     4081 gcgatgcgga tgctcgacaa cgtcatcgac atcaactact acgcggtcgc gaaggcgaga
     4141 aactcgaacc tgcgccaccg tccggtcggg ctgggcatca tggggttcca ggactgcctg
     4201 caccgcctgc ggattgcgta cgcctcgagc gaagcggtgg agttcgccga ccgttcgatg
     4261 gagatggtgg cgtactgcgc ctactgggcc tcgaccgagc tcgccgagga gcgcggccgc
     4321 tacgcctctt tccagggctc gctgtgggac cgcggcgtgc tgccgcagga cacgctcgac
     4381 ctcctcgccg ccgagcgcgg cggctacgtc gagctcgatc gctcggcgac gctcgactgg
     4441 gaggcgctgc gcgcgcgcat caagcgtcac ggcatgcgca actccaattg cctcgccatc
     4501 gcgcctactg cgaccatcgc caatatcgtc ggcgtgtcgg cctcgatcga gccgacctac
     4561 cagaacctgt tcgtcaagtc gaacctgtcg ggcgagttca ccgtcgtgaa cgagtacctc
     4621 gtcgtcgacc tgaagcaggc cggcctgtgg gacgaggtga tgatcgcgga cctgaagtat
     4681 tttgacggca acctcgcgcg catcgaccgc gtgccagcgg cgctgcggcg gctgtacgcc
     4741 acggccttcg aagtcgagcc gaagtggctg gtcgaggcgg ccgcgcggcg ccagaagtgg
     4801 atcgaccagt cgcagtcgct caacatctac atggccggcg cgtcgggtaa gaagctggac
     4861 gaaacctaca agctcgcctg gatccgcggc ctgaagacca cctactacct gcgctcgatg
     4921 ggcgcgacgc acgcggagaa atcgaccagc aaggcgggcc agctgaacgc ggtgtcctcg
     4981 ggcggggagg ccgaggaggc gaaagtgtgc gccatcgaca actcggagtg cgaggcctgc
     5041 caatgaaact agagaacctc gcactgcagc cgccgggcgg cttcgtgccg gccacaggag
     5101 cgagtgccgg agctgcggcg gagcgttttc aggtggggaa cgcctgccgg cgggtcacgg
     5161 tcgaggacaa gagcgtcatc aactccaagg ccgacgtcaa ccagctggtg cccttcaagt
     5221 acaagtgggc ctgggagaag tacctcgccg cctgcgccaa ccactggatg ccgcaggaga
     5281 tcaacatgag ccgcgacatc gcgctgtgga aggacccggc gggcctcacc gaggacgagc
     5341 gccgcgtcat caagcgcaat ctcggtttct tcgtcaccgc cgattcgctg gcggccaaca
     5401 acatcgtgct cggcacctac cggcacatca ccgcgccgga atgccgccaa tacctgctgc
     5461 gccaggcgtt cgaggaggcg atccataccc acgcctacca gtacatcgtc gagagcctcg
     5521 acctcgacga aagcgagatc ttcaacgcct accacgagat ccactcgatc cgcgacaagg
     5581 acgagttcct ggtgccgttc atcgacaccc tgaccgaccc gcatttcacc accggcacgc
     5641 cggcgaacga ccaggcgctg ctgaagagcc tgatcgtgtt ttcctgcctg atggagggcc
     5701 tgttcttcta cgtcggcttc acgcagatcc tttcgctcgg caggcagaac aagatgaccg
     5761 gcgcggccga gcagtacatg tacatcctgc gcgacgagtc gatgcactgc aacttcggca
     5821 tcgatctcgt caaccagatc aagctcgaga acccgcagct gtggaccgcg tcgttccgcg
     5881 aggagctcgc ggacatcttc cgccgcgcgg tcgagctcga ataccgctac gccgaggaca
     5941 ccatgccgcg cggcgtgctc ggcctcaatg cgccgatgtt caaggagtac ctgcggttca
     6001 tctgcaaccg ccgctgccag cagatcggcc tggagaccct ctacccggga gccagcaatc
     6061 cgttcccgtg gatgagcgag atgatcgacc tgaagaagga acgcaacttc ttcgagaccc
     6121 gcgtcatcga gtaccagacc ggcggggcac tgagctggga atagaggcca aggggcgagg
     6181 agcaatcgtg gaagaggaat gggacgacat gaagatcacg gcatgcaagg ccgaggacga
     6241 ctacaagctg tggctgcgct tcgaggacgg cctcgagggc agcgtgtttc tcggcaacct
     6301 gctcgagatc ggcgccttcc agctgtggcg cgacgtgcgc gagttcgaga aggtgagcgt
     6361 cgaccccgag accgcgaccg tcacctggga aggcggtata cgcctcgacg ccgatatcct
     6421 ttactatgat ctaaagaaca atccaattca gcacgccgcg gcggcaagtc cgccgtcggt
     6481 ggttgaatag tccgggccgg cacggccggg ctcttttgtt caacaacctt gaaggagatt
     6541 gagaatggcg aagaaaaaag cgaaaaaagg cggcaagaaa aagaagaaga agaaagcatc
     6601 acgaaagaag aagaagtcgt tgaaaagagc tggcaagaag aaggcggcag gcagaaagaa
     6661 atccgccaag aaaaagacga aggcgggaaa gaagaaaaag gcgaaagccg ccaaacctga
     6721 ggccagccct gcggcaacga tggcggcgag tccagctgca ccgccatcca ccgctgcgac
     6781 acccggcgcg agaaccgcgc tgaatccagc ggccgcgtgg ccatttccca ccggctcccg
     6841 gccgtaaccg ggaaccgggg gaacatggcg gggggcgcaa gcccctcgcc aggccctcga
     6901 agctcagcgg accggcttgg ccaatcggcc caagatcgag ttctaggccg gtccgcgagg
     6961 cttcaccaga agccgcaagg cttcaccaga ggttcgcaag gcttcaccag aggtctgcaa
     7021 ggcctcactt cagggcctat tttaccgggt tgcagcactg cgccaggcag ggaaccaggc
     7081 agggaaaaag gatgtcgaaa caaacgtttc gcagcgcctt ggcgcgctgg tacgaggaga
     7141 agggaagtca ttgacagagt aaggatattt ttcttatgat gccttccgct gcaccgcagc
     7201 attctgctcg gctcaagggc gttttactgg cgctctcgag aaccctccct caagccgcaa
     7261 ttcaaccact acggtagcac cacggcggcg cagggaagca cggaggttga cgatgactac
     7321 gacgctagtg gctgcacgcg cttacgccgc gcttaaagga ggcctcgctc tggtagggct
     7381 cgcggcgctg gccgcattct tcgttttgcc cactcggccc gatgccgtct tgccgcagct
     7441 tttcagcctg gcaggcgatg ccttcggcga gaacccgacg gtcgctgccg gcccgatcca
     7501 ggcccgcatg gagacgccgc tcgagcgcga gcagcgcgcg atgaccgcat acatagccaa
     7561 gcgctaccgg gtcgccgaca ccgccgtcgc cggcttcgtc accgcggcgt atcgctccgg
     7621 cgagcagtac tccgtggatc cgctgctgat ccttgccgtg atggcggtcg agtcgcgcta
     7681 caaccccgtc gccgagagca cggtcggcgc gaagggcctg atgcaggtga ttcccaagtt
     7741 ccacctggaa aagctcatgg atcacggcgg cgagcaggcg ctgctcgagc ccgaggtcaa
     7801 catccaggtc ggcgcgcgca tcctgcgcga gtaccagcgg cgcttcggcg acaccgagac
     7861 tgcgctgcag atgtatgccg gtgcgttcga cgagccgacc gcgcaatacg cctccaaggt
     7921 gttcgccgag aaggcgcggt tcgagtccct caggcagttg ctcaggcaga aagccaggaa
     7981 gcagcaaagc gtgtaacgcg cgccgcggcc tcctcgagct ccgcgaggcc gcgggtgtag
     8041 gcgaagcgca cgaaacgcgc ggtcgcattg gcaccgaaat cgatccccgg cgtcgcggcg
     8101 accgcctctt tctcgagcag ctccaggcag aagcgcctcg cgtccccgcc gcagtcggcg
     8161 tagacataga aagcgccgtg cggctcggca ggcaccgcga gcccagcctg gcgtagcgcg
     8221 ggaaccagga agtcgcgccg tttttcgaat tcacttcttc gaccttctag aattttcagg
     8281 gattgaggag agaaagcgct gcacgccgca tgctgcgcgg cagaaggcgc cgagatgaag
     8341 agatgctggg cgagcttctc gaagccgcgt accgcgcctt caggcagcac tacccagccc
     8401 aggcgccatc cggtcatgca gaagtacttc gagaagctgt tgatcaccac cacctcgccc
     8461 ggcaggccga gcgcgctcca gggctcgatg ccgtacacca gcccctggta gatctcgtcg
     8521 accagcagca cgctgccgcg ctcggcgacg aagtgcgcaa ttttctccaa ttccgttttc
     8581 gaaatgagcg ttccggtcgg gttggacggc gagccgagca gcaggccctt ggtgcgcgcg
     8641 ctccaggcgg aacgcaccat gtcgagcgtc ggctggaaat tttgcgccgc cgacaccggc
     8701 agggtgcgcg cgacaccttc gaaggcgcgc acgaaatgac gatagccggg atagccgggg
     8761 tcgggcagca ggatctcgtc accgggatcg acgtacaggg ccgtgaccgt gagcaggccg
     8821 ccggatgcgc ccgatgtgat cgcgatgctg tccgcaggga tgtttttcga aaatctggct
     8881 tcgtagtgcg ccgcgatcgc tgcgcgcagc tgcggcagtc cgagcgtcgc cgtataagcc
     8941 gtgagcccgt cgcgcagggc gcgcacgccc gcttcgacca ccggggcggg cgcggtgaag
     9001 tcgggctcgc cgatctccat gtggatcacc ttgcgcccgg cggcttcgag cgcgtgcgcg
     9061 cgtgccagca cgtccatgac ttcgaacggc tcgattgccg cgacacggcg cgccgcgaga
     9121 ttcgccacag gggtcggcat gggggtgagg gattataatt ttttggtgga cgagcaactg
     9181 aagaacaacg ctctcgagta tcaccggcgc agcccgcccg gcaagatctc gattcttccc
     9241 accaagcagc tcaccaacca gcgcgatctc gctctcgcgt actcgcccgg ggttgcggcg
     9301 gcgtgcgaag agatcgtgcg cgatgccgcg caggcggttc gctataccgc gcgcgccaac
     9361 ctgatcgcgg tgatcaccaa cggcactgcg gtcctgggtc tgggcgcgat cggcccgctc
     9421 gcggcgaagc cggtgatgga aggcaagagc gtgctgttca agaaattcgc cggcatcgac
     9481 tgcttcgaca tcgagctcga cgagcgcgat ccgcagcgcc tggtcgacat catcgcggcg
     9541 ctcgagccca cgttcggcgc catcaacctg gaggacatca aggcgccgga gtgcttctac
     9601 atcgaggaga cgctccgccg caccatgaag atcccggtat tccatgacga ccagcacggc
     9661 accgccatca tttccggc
//
LOCUS       DASPXW010000133         9925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1678239, whole genome shotgun sequence.
ACCESSION   DASPXW010000133 DASPXW010000000
VERSION     DASPXW010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9925
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1678239"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VEL04_03170"
     CDS             complement(<1..491)
                     /locus_tag="VEL04_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006498753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HYT47396.1"
                     /translation="MIIFIAGVAVTACLGYMTAMPGRSHIGPLPPLSGEGRALSLRLR
                     LHVAALAAAERNSDLETPARYIEAAFSRVGLDPQSQYFESGGRRVRNIEVGSGAIVVG
                     AHYDTVPGSPGADDNASGVAVLIELSRLLATQGLPIRFVAFASEEMPYFLTEEMGSSA
                     WAKR"
     gene            complement(508..1653)
                     /locus_tag="VEL04_03175"
     CDS             complement(508..1653)
                     /locus_tag="VEL04_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HYT47397.1"
                     /translation="MKFETKVIHAGQAPDPQTGAVMTPIYATSTYAQSSPGEHKGFDY
                     ARTRNPTRDALEACLAELESGHAAFAFASGMAAIATTLELLDAGSHIVAMHDLYGGSY
                     RILEKVRKRSANLQCSFVDLSDVSVLQKAIQSNTRMLWIESPTNPLLKLVDLEAAAAL
                     ARKHRLLSVCDNTFASPWIQRPLEHGFDIVVHSTTKYLNGHSDVIGGAVIIGNDPSLK
                     EKLSFLQNAVGGVPSPFDCFLTLRGIKTLALRMERHCGNAMHVAAFLEKHPKVSRVIY
                     PGLASHPQHLLAARQMNRRYGGMVTAVLKGGLAASRRFLERCKLFTLAESLGGVESLI
                     EHPAIMTHASLPEDVRAELGIDAGLVRLSVGIESVDDLIAELKHALE"
     gene            complement(1650..3014)
                     /locus_tag="VEL04_03180"
     CDS             complement(1650..3014)
                     /locus_tag="VEL04_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HYT47398.1"
                     /translation="MKDVLHLIGNTPMIETRRLVAGPCRLFLKLENQNPGGSIKDRIA
                     LSMIEAAERAGQIKPGFLIVEATAGNTGLGLALVAAQKGYRLLIVVPDKMSQEKIFHL
                     KALGAEVVLTRSDVTKGHPDYYQDRAARLARETPGAFYVNQFANAANPRAHEQTTGPE
                     IWEQMQHALDAMVCGVGTGGTITGLARYFERASPNTQMILADPQGSVLADYVKTGKLR
                     KEVGSWLVEGIGEDFLPPVCDLSRVKKAYSIPDKEAFLTCRELLKSEGIMAGTSSGTL
                     VAAALRYCREQTEPKRVVTLICDSGNKYLSKVYNDFWMIDQGFIERERSGDLRDLIAR
                     RHRERAVATVGADETVLAAYQRMKLYDVSQLPVMRGGRIVGIVDEEDILLEVYDNPGH
                     FDEAVTEAMESHLVTVPPNAPVAQLMEIFKRGLVAIVVDGDEFLGLVTRIDLLNWLRR
                     RREA"
     gene            3127..4536
                     /locus_tag="VEL04_03185"
     CDS             3127..4536
                     /locus_tag="VEL04_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47399.1"
                     /translation="MQAAGGPTLLGSISDFCRRTGMAESSFGRRAVNDGKFVARLRDG
                     ARVTPETLQRVNQFMTKHGLAGAGDSPPELLALIRASAAQPAAAAASSSREAPEKNFR
                     FFDNRQKYLLFVNTCSEKDVVARRVGLELGHIHPRPPAIRLFDAGMGDGTVLARVMRE
                     MHRRFATVPFYIVGKEISLEDVRLSLRKMVDRFYEHPATVLVVTNMYYTEAPWLTPRT
                     VPAATSMVWHEAGLTGSTSHDFAEQIATLEPFLAKNWQARHSPKTGNPVYEHPVALVI
                     YREDYRFLLENVMPKRGEGRAEYDMVIASQPYRLRAPLEFKAQKVIAPLVRALGKGGR
                     LLGIHSYGHDPGLEIIQKVWPGENPYTQSRHDVLRAAKAELGKDARRYNFNAYADARA
                     LFRYDMHTLPSEISASIGTSTLFAAWNAAIYVAQIDDARLGDALRDTRYLDATREILQ
                     KHGSLWFLDESYVVSRKRD"
     gene            4544..5098
                     /locus_tag="VEL04_03190"
     CDS             4544..5098
                     /locus_tag="VEL04_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47400.1"
                     /translation="MKSRISELVAAGSLEISPRELHRAGEVAALLPAGTCVYIPSLPG
                     LPLPRTLETVAALRAAGLDPVPHISARRVLDRGAFRSFLNAATSRYGVHRVLLVGGDE
                     PRAKGPYADSLEVLQDGVLADAGIREIGVAGYPEGHPRAGQRGRRAPLQLRRRGAHRS
                     LDARAHRRLAANRGYRFHGMHVKV"
     gene            complement(5048..7552)
                     /locus_tag="VEL04_03195"
     CDS             complement(5048..7552)
                     /locus_tag="VEL04_03195"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HYT47401.1"
                     /translation="MRQPTAWTILVVGVVLSLLTWRALNVEVEHAARTSFNAAVAESR
                     TAIESRLRGYRIMLYGLQGLFHASTEVDRAEFRRYFEHLAAAGGSGQLRGFSYARRVS
                     HAQKKSFEAAVRSDSSLQPGGYPRFSIKPPGERQEYVVAHLVEPFSEAGFGLDIIADP
                     VRRAAVERARDTGELVATAPFALLSSPSGEAGTSLRLAVYRHGATLDSLESRREAFAG
                     VVGATFAIRDLVADIVSRHAAGSLQLLIRDGSEILHDSATAGLPADAFRTMTPLEVGG
                     RVWQLQFSAPQQRFRTAGEVLMPWLALLGGTAITVLLAGLVGSLATSTRRARRIADTI
                     TQDLLMSQAKLADAQRKTQTLIETLPNPVFFKGTDGRYLGVNKAWEAFFNTPRAAIIG
                     KTVQDLYTHAADVAERLRAMDEVLWQNPGTQTHETAITLPDGTQRDTVYYKATFTGPD
                     GGVAGLIGTIVDITERKQAEQRQAMEHAVTQVLAKAESLSEAAPRIIQTICETLGWAC
                     GAHWRWDEPAQVLRCAETWHVDAAEVAQFISATSATINEAPAWHGEAPRTKSGGLVRR
                     IWLDGVPVWFPDVTREPGFRRGPDAAKAGLHCAFGFPVMAGAQPLGVVEFFGRDIKQP
                     DSALLSIAQSIGSQIGQFIVRKQAEEALRFVATHDALTGLPNRVMFGQRLDHAMRRAQ
                     RRGQRLAVLFIDLDRFKEINDALGHDYGDSVLREVAHRFGQGLRGSDSVARFGGDEFV
                     VLLEEIADPMPVAGVAQKLGAALAERFVLAGSEYQVTASIGVSVYPDDSEDAQALLKN
                     ADIAMYRAKERGGNTFRFYSSHLNVHTVEPVSSVRR"
     gene            complement(7628..8383)
                     /locus_tag="VEL04_03200"
     CDS             complement(7628..8383)
                     /locus_tag="VEL04_03200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYT47402.1"
                     /translation="MTSNTKVALVTGAGTGIGKAAALAMLKDGYKVALVGRRKEMLDK
                     TAADSGAKDRALVLPADITKPEEVKRIFLKIKQSWERLDVLFNNAGMGAPAVPMEDLP
                     LEKWREVVDLNLTAMFMCTQEAIRIMKSQQPRGGRIINNGSISAHAPRPMSVAYTSTK
                     HAVTGLTKCISLDGRKHDIAASQIDIGNALTELAARMAKGVPQADGSVRPEPLMDVAH
                     VGAAVLHMANLPLDVNVQFMTIMATKMPFVGRG"
     gene            complement(8453..9814)
                     /locus_tag="VEL04_03205"
     CDS             complement(8453..9814)
                     /locus_tag="VEL04_03205"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HYT47403.1"
                     /translation="MRLALPALMLLALGAALLVGLPVGVVLIAVAFLFAGLGVMAGLV
                     RSDDIGAIFLRIYGTLSDRDEIVYAAVPMLLFMGAILHETGLAADLLAGVTRAFGRMR
                     GGPAVATIAVGALQAPAAGMIGASTGALALYALPALERQGYRTEQAAGLVAAAGTLGV
                     IAPPGIMLFFIADAIGAQVPALFLAMLGPLALLLLLYLGYSIMLGSPAAAPATPQTKG
                     VFAGALLCAGLMVALVGIVASGLATLSEAAALAAVGAVLAGAARGRVTWRALDVAIRR
                     TALIGAMVFFIFAGASVFSLVFRLLGGQEMVSTAAAMLGSRGASALLAALVFVFILGF
                     FLDWLEIVVIALPVLAATLIAGIAERLGNAQLAACWLGALFAINLQTSFLTPPFGYAL
                     FLVHGATGGRAGLFEVWRGALPYLALQLVTLVAVLAIPELATWLPGQLLDLSVPKGPK
                     FNE"
BASE COUNT         1675 a         3287 c         3293 g         1670 t
ORIGIN      
        1 cgcttcgccc aggccgagct tcccatttcc tcggtcagga agtaaggcat ttcctcgctg
       61 gcgaaagcga cgaagcggat cggcaacccc tgtgtcgcga gcagcctcga gagctcgatc
      121 agcacggcga cgccggaagc gttgtcatct gcgcccggcg agcccgggac ggtatcgtag
      181 tgcgcaccga ccacgatcgc gcccgagccg acctcgatgt tgcgcaccct gcggccgccg
      241 ctttcgaaat actgcgactg cgggtcgagg ccgacgcgcg aaaacgccgc ttcgatatag
      301 cgcgccggag tttcgaggtc cgagtttctc tcggcagcgg cgagcgcggc gacgtgcagc
      361 cgcagtcgca gggacagcgc gcggccttcc ccggacagcg gcggcagcgg cccaatgtgc
      421 gagcggccgg gcatcgccgt catgtagcca aggcaggcgg tgaccgcgac gcctgcgata
      481 aagataatca agactaaagt gatgggatta ctcgagcgcg tgtttcagct cggcgatgag
      541 atcgtcgacg ctttcgatgc cgaccgacag gcgcaccagg cccgcgtcga tgccgagctc
      601 ggcgcgcacg tcctcgggca gcgacgcatg ggtcatgatc gccgggtgct cgatcaggct
      661 ttcgacgccg ccgaggctct cggcaagcgt gaacagcttg cagcgctcga ggaagcggcg
      721 cgacgccgcg aggccgcctt tcagcaccgc ggtcaccatg ccgccgtagc gccggttcat
      781 ctgccgcgcc gcgagcaggt gctgcgggtg gctggcgagc ccgggataga tcacgcgact
      841 cactttggga tgcttctcga ggaatgcagc gacgtgcatc gcgttgccgc aatgccgctc
      901 catgcgcagg gcgagcgtct tgatgccgcg cagcgtgagg aagcagtcga aggggcttgg
      961 cacaccgccc acggcatttt gaagaaaaga caatttttcc ttaagggaag gatcgttgcc
     1021 aatgattacc gcgccaccta tgacatcgga atgcccattc aggtacttgg tggtcgagtg
     1081 caccacgatg tcgaagccgt gctcgagcgg gcgctggatc cacggcgagg caaaagtgtt
     1141 atcgcacacg gaaagcagcc tgtgcttccg ggcgagcgcg gctgcggcct cgagatcgac
     1201 gagcttcagc agcgggttgg tcgggctctc gatccacagc atgcgggtgt tggactggat
     1261 tgctttttga agaacgctta catccgataa atcgacgaag ctgcactgca aattggcgga
     1321 ccgcttgcgc actttttcga ggatgcggta gctgccgccg tagaggtcgt gcatggcgac
     1381 gatgtgcgag ccggcgtcga gcagctcgag ggtggtggcg atcgccgcca tgccggaggc
     1441 gaaggcgaac gcggcgtggc cgctctcgag ctcggcgagg caggcctcga gcgcgtcgcg
     1501 cgtcgggttg cgcgtgcgcg cgtagtcgaa gcccttgtgc tcgcccgggc tcgactgtgc
     1561 gtacgtcgag gtggcgtaga tcggcgtcat caccgcgccg gtctgcgggt cgggcgcctg
     1621 cccggcgtgg atcaccttgg tctcgaactt catgcttcgc gccttctgcg cagccagttg
     1681 aggaggtcga tgcgcgtgac gagaccgagg aactcgtcgc cgtcgaccac gatcgcgacc
     1741 aggccgcgct tgaagatctc catcagctgc gcgaccggcg cgttgggcgg cacagtgacg
     1801 aggtggctct ccatcgcctc ggtcaccgcc tcgtcgaaat gccccggatt gtcgtatacc
     1861 tcgagcagga tgtcctcctc gtcgacgatg ccgacgatcc tgccgccgcg catcaccggg
     1921 agctgcgaca cgtcgtagag cttcatgcgc tggtaggcgg cgagcacggt ctcgtcagcg
     1981 ccgacggtgg caaccgcccg ctcgcgatgg cgccgggcga tcaggtcgcg caggtcgccc
     2041 gagcgttcgc gctcgatgaa gccctggtcg atcatccaga aatcgttgta caccttggaa
     2101 agatatttgt tgccgctgtc gcagatcagc gtgacgactc gcttcggctc ggtctgctcc
     2161 cggcagtagc gcaacgccgc cgctaccagc gtccccgagg aggtgcccgc catgatgcct
     2221 tcgctcttca ggagctcgcg gcaggtgagg aacgcttcct tgtccggaat gctgtaggcc
     2281 ttcttgacgc gcgagaggtc gcatactggc ggcaggaaat cctcgccgat gccttcgacc
     2341 agccagctgc cgacctcctt tctcagtttc ccggttttca cgtaatccgc cagcactgag
     2401 ccctgcggat cggcgaggat catttgagtg tttggcgagg cgcgctcgaa gtagcgcgcg
     2461 aggccggtga tggtaccgcc cgtacccacc ccgcagacca tggcgtcgag ggcgtgctgc
     2521 atctgctccc agatctccgg cccggtggtc tgctcgtggg cccggggatt ggcggcattg
     2581 gcgaattgat tgacatagaa cgcgccgggc gtttcgcgcg cgaggcgcgc ggcgcggtcc
     2641 tggtaataat ctggatggcc cttggtcacg tccgagcgcg tcagcaccac ttccgcaccc
     2701 agcgccttca agtgaaaaat cttttcctgg ctcatcttat cgggcacgac gatcagcagc
     2761 cggtagcctt tctgcgccgc caccagcgcg agcccgagcc ccgtgtttcc ggcggtcgcc
     2821 tcgacgatca ggaagcctgg tttgatctga ccggcgcgct ctgcagcctc gatcatggac
     2881 agcgcgatgc ggtccttgat cgagccgccc gggttctggt tctcgagctt gaggaacagc
     2941 cggcaaggcc cggcgacgag ccggcgcgtt tcgatcatcg gggtgttgcc gatcaaatgc
     3001 aggacatctt tcatgtaagt ccttgttgcg taaaggtggg atatcgccca cattttgcag
     3061 tgcacatcat gtggttgatg tcccacaaca ccttgcccac tatggaacgg tttcgaggag
     3121 gagtccatgc aggccgcggg cggcccgacc ttacttggca gtatctcgga tttctgccgg
     3181 cgcaccggga tggcggagtc gtcgttcggc cggcgcgcgg tcaacgacgg caagttcgtg
     3241 gcgcggctgc gcgacggcgc gcgcgtgacc ccggagacat tgcagcgggt caaccagttc
     3301 atgacgaagc acggactcgc cggcgcaggc gattcgccgc ccgagctgct ggcgctgatc
     3361 cgcgccagcg cagcgcagcc cgccgcggcg gcggcgtcgt cgtcgcgcga ggctccggaa
     3421 aagaacttcc gcttcttcga caaccggcag aagtacctgc tgttcgtcaa tacctgcagc
     3481 gagaaggacg tggtcgccag gcgggtgggt ctggagctcg gccacatcca tccgcgcccg
     3541 ccggcgatcc gcctgttcga cgccggcatg ggcgacggca ccgtgcttgc acgcgtgatg
     3601 cgcgagatgc accgccgctt tgccacggtg ccgttttaca tcgtcggcaa ggagatcagc
     3661 ctggaggacg tgcggctgtc gctgcgcaag atggtcgatc gcttctacga gcacccggcg
     3721 accgtgctgg tggtcaccaa catgtactac accgaggcgc cgtggctcac gccgcgcacg
     3781 gtccccgcgg cgacctcgat ggtgtggcac gaggcgggcc ttaccggctc gacctcgcac
     3841 gacttcgccg aacagatcgc gacgctcgag cccttcctcg ccaagaactg gcaggcgcgc
     3901 catagcccga agacgggtaa cccggtctac gagcatccag tggccctggt gatctaccgc
     3961 gaggactacc gctttctgct cgagaacgtg atgccgaagc gcggcgaagg gcgtgccgag
     4021 tacgacatgg tgattgcctc gcagccttac cgcctgcgcg cgccgctcga attcaaggcg
     4081 cagaaggtca tcgcgccgct ggtgcgcgcg ctcggcaagg gagggcggct gctcggcatc
     4141 cattcctacg ggcacgaccc gggcctcgag atcatccaga aggtctggcc gggcgagaat
     4201 ccgtacacgc aaagccggca cgacgtgctg cgcgcggcga aggccgagct cggcaaggac
     4261 gcgcggcgct acaacttcaa cgcctacgcc gacgcgcgcg cgctgttccg ctacgacatg
     4321 cacacgctgc cgagcgagat ttcggcgagc atcgggacct ccacgctgtt cgccgcctgg
     4381 aacgccgcca tctacgtcgc gcagatcgat gatgcgcggc tgggcgacgc gctgcgcgac
     4441 acgcgctacc tcgacgcgac gcgcgagatc ctgcagaagc acggcagcct gtggttcctc
     4501 gacgagtcct atgtcgtttc ccgcaagcgc gactgaagct cccttgaaat cacgcatctc
     4561 cgagctggtc gcggcgggct cgctcgagat ttcgccgcgc gagctgcacc gcgccgggga
     4621 agtggccgcg ctgctgcctg ccgggacctg cgtctacatc ccgtcgcttc cggggctgcc
     4681 gctgccgcgc acgctcgaaa ccgtggccgc gctgcgcgcc gccgggctcg acccggtgcc
     4741 gcacatctcc gctcgtcgcg tcctcgatcg cggcgcattc aggtcttttt tgaacgcggc
     4801 aacttccagg tacggcgtgc atcgcgtact tctcgtcggc ggcgacgagc cgcgggcgaa
     4861 aggaccgtat gcggacagcc tggaagtgct gcaggacggc gtgctcgccg atgcgggcat
     4921 ccgcgagatc ggggtcgccg gctaccccga ggggcatccg agggccgggc aacgtggccg
     4981 gcgtgcacct ctacagcttc ggcggcgcgg cgcgcaccgc agcctggatg cgcgagcgca
     5041 tcgccggcta gcggcgaacc gaggataccg gttccacggt atgcacgtta aggtgtgacg
     5101 agtagaaccg gaacgtgttg ccgccccgtt ccttggcccg gtacatggcg atgtcggcgt
     5161 ttttcagcag cgcctgcgca tcttccgaat cgtcgggata gacgctgacg ccgatgctgg
     5221 cggtgacctg atattcgctt ccggcgagga cgaagcgctc ggcaagcgcg gcgcccagct
     5281 tctgcgccac gccggcgact ggcatcgggt cggcaatctc ctcgagcagc acgacgaact
     5341 cgtcgccgcc gaaccgcgcc acgctgtcgc tgccgcgcag gccctgaccg aaccggtgcg
     5401 cgacttcgcg cagcacactg tcgccgtagt catgcccgag agcgtcgttg atctccttga
     5461 agcggtcgag atcgatgaac agcacggcga ggcgctggcc gcggcgttgt gcccggcgca
     5521 tcgcgtgatc gaggcgctgg ccgaacatca cccggttcgg caggcctgtg agcgcgtcgt
     5581 gcgtcgccac gaagcgcagc gcttcctcgg cctgcttgcg tacgatgaac tgcccaatct
     5641 ggctgccgat cgactgcgcg atcgacagca gcgcgctgtc gggctgcttg atgtcgcggc
     5701 cgaagaattc caccaccccc aatggttgtg ctccggccat gaccgggaat ccgaaggcgc
     5761 agtgcagacc tgccttggcg gcatccggtc cgcgccgaaa acccggctcc cgcgtgacat
     5821 cggggaacca caccggcacg ccgtcgagcc agatgcgtcg gaccaggccg ccgctcttgg
     5881 tcctcggggc ctcgccgtgc caagccggcg cctcgttgat ggtggctgaa gtcgccgaga
     5941 tgaattgcgc gacctccgca gcgtcgacat gccatgtttc ggcgcagcgc aacacctggg
     6001 ccggttcatc ccatcgccag tgagcgccgc aggcccatcc gagggtttcg cagatggtct
     6061 gaatgatcct gggagccgct tcgctgaggc tttccgcttt cgccagcacc tgcgtaacgg
     6121 catgctccat cgcctggcgc tgctccgcct gcttgcgctc ggtgatgtcg acgatggtgc
     6181 cgatgagccc cgcgacgccg ccgtcgggac cggtaaaggt ggccttgtaa tatacggtat
     6241 cgcgctgcgt gccatcgggc agggtgatgg cggtttcatg ggtctgggtg cccgggttct
     6301 gccacagcac ttcgtccatc gcgcgcaggc gttcggctac gtccgcggca tgggtgtaga
     6361 ggtcttgcac tgtcttgccg atgatcgcgg cgcgcggggt attgaaaaac gcctcccagg
     6421 ccttgttgac gccgaggtag cgtccatcgg tgcccttgaa gaagaccgga ttgggcagcg
     6481 tttcgatcag cgtctgggtc ttgcgctgcg cgtcggcgag ctttgcctgg ctcatgagca
     6541 gatcctgcgt gatggtgtct gcaatgcgcc gcgcgcgccg ggtggaggtg gcgagcgagc
     6601 ccaccagccc ggcgagcagc acggtaatgg cggtcccgcc aagcagtgca agccagggca
     6661 tgaggacctc gccggcagtt cggaagcgct gttgcggcgc gctaaactgc agttgccaca
     6721 cgcgcccgcc gacctcgaga ggcgtcatcg tccggaatgc atcggccggc agccccgcgg
     6781 tcgcgctgtc gtgcagtatc tcggagccgt cgcggatcag aagctgcagc gaccccgccg
     6841 cgtgtcgcga cacgatgtcc gctacgagat cgcgaatcgc gaacgtcgcg ccgactacac
     6901 cggcgaatgc ctcgcgccgc gattcgaggc tgtcgagcgt cgcgccgtgg cggtaaacgg
     6961 cgaggcgcag gcttgttccc gcctcgcccg acggcgagga gagaagtgcg aaaggcgcgg
     7021 tcgcgaccag ctcgccggtg tcgcgcgcgc gctcgaccgc cgcccggcgc accggatcgg
     7081 cgatgatatc gagcccgaag cccgcttcgc tgaagggctc gactagatgg gccaccacgt
     7141 actcctggcg ctcgcccggc ggtttgatcg agaatcgggg atatccaccc ggctggagac
     7201 tgctgtcgct gcgcaccgcc gcctcgaagc tctttttctg cgcgtgcgaa acgcgccgtg
     7261 catagctgaa cccgcgcagc tgaccggagc cgcccgcggc ggcgagatgc tcgaaatagc
     7321 ggcggaattc cgcccggtcg acctcggtac tggcgtggaa cagcccctgg aggccgtaca
     7381 gcatgatgcg gtagccgcgc aggcgcgatt cgatcgcagt gcgggactcg gcgaccgcgg
     7441 cgttgaagct ggtccgcgcc gcgtgctcga cttcgacgtt gagcgcgcgc caggtgagga
     7501 gcgacagcac cacgccgaca accaggatcg tccatgcggt cggctggcgc atccagtcac
     7561 gaaactcggc gctgtcccac cgcaagggca cctccctccc gcgggctgat ttatccgatt
     7621 ctaggcttca gcctctgccg acgaaaggca tcttggtcgc catgatggtc atgaactgca
     7681 cgttgacgtc gagcggcagg ttcgccatgt gcagcaccgc ggcgccgaca tgcgccacgt
     7741 ccatcagcgg ctcgggcctg accgagccgt ccgcctgggg aacgcctttc gccatccgcg
     7801 cggccagctc ggtgagcgca ttgccgatgt cgatctggct tgccgcgatg tcgtgcttcc
     7861 tgccgtcgag cgagatgcac ttggtcaggc ccgtgaccgc gtgcttggtg gaggtgtagg
     7921 cgaccgacat gggccggggc gcgtgcgccg agatcgagcc gttgttgatg atgcggccgc
     7981 cgcgcggctg ctggctcttc atgatgcgga tcgcttcctg cgtgcacatg aacatcgccg
     8041 tcaggttcag gtcgacgact tcccgccatt tctccagcgg cagatcttcc atcggcaccg
     8101 caggcgcgcc catgccggcg ttgttgaaca gcacgtcgag gcgctcccac gattgtttta
     8161 ttttcaggaa gattcttttt acctcttcgg gcttggtgat atccgccggc agcaccagcg
     8221 cacgatcctt agcgcccgag tccgccgcgg tcttgtccag catctccttg cggcggccga
     8281 cgagcgccac cttgtagccg tccttcagca tggcgagcgc cgcagccttg ccgatgcccg
     8341 tgcctgcgcc cgtaaccagc gcgaccttgg tattcgaagt catgggatct ccattgctga
     8401 tgcaggaatt tgccgaggcc gcgatggtag cgcagcgaac gcctcgtatc cgtcattcgt
     8461 tgaactttgg gcctttgggc accgacaagt ccagcaactg cccggggagc caggttgcga
     8521 gctcgggaat cgccagtacc gccaccagcg tgaccagctg caaggcgagg tagggaagcg
     8581 cgccgcgcca cacttcgaac aggcctgccc tgccgccggt agcgccgtgc accaggaaca
     8641 acgcgtagcc gaacggtggc gtcaggaacg aggtctgcag gttgatggcg aacagagcgc
     8701 cgagccagca tgcggcgagc tgggcattgc ctaaccgctc cgcgattccc gcgatgagag
     8761 tcgcggcgag cacagggagc gcgatcacca cgatttccag ccagtcgagg aaaaagccca
     8821 ggatgaatac gaataccagc gcagcgagta gcgcgctggc gccgcggctt cccagcattg
     8881 ccgcggcggt cgaaaccatc tcctgcccgc caagcagccg gaacaccagt gagaacacgc
     8941 tcgcgcctgc gaaaatgaag aacaccatgg cgccgatcag tgcggtgcgc cggatggcca
     9001 cgtccagcgc tcgccatgtc acacgcccgc gcgccgcgcc cgcaaggacg gctcccacag
     9061 cggcgagcgc cgcggcttcc gaaagggtcg ccagtccaga tgcgacgatg cccaccagcg
     9121 ctaccatcag gccggcacag agcagtgcgc ccgcaaacac tcctttggtt tgcggcgttg
     9181 cgggcgccgc cgcaggcgat cccagcatga tcgaataccc gagatagagc agcagtagca
     9241 aggcgagcgg accgagcatg gcgagaaaca aggccggaac ctgtgcgccg atcgcgtccg
     9301 cgatgaaaaa cagcatgatt cccggtggcg cgatgacgcc cagcgttcct gccgccgcca
     9361 ccagtccggc cgcctgttcc gtgcgatagc cctgacgctc gagcgccggc agcgcgtaga
     9421 gcgcgagcgc gccggtcgaa gcgccgatca tgccggcggc gggcgcctgc aaagcgccca
     9481 ctgcaatggt ggctacggcc gggccgccgc gcatgcgccc gaaggcccga gtgacgcccg
     9541 ccaggaggtc cgcggccagc cccgtctcgt gcaggatcgc tcccatgaag agcagcatcg
     9601 gcacggccgc atacacgatc tcatcccggt ccgacagcgt gccgtagatc ctcagaaata
     9661 tcgcgccaat atcgtcgctg cgaaccaatc cggccatcac gccgagaccg gcgaagagga
     9721 acgccacggc aatcagcacg acgccgacgg gcaggccgac caagagcgcc gcgccgagcg
     9781 ccagcaacat gagcgccggc aaggcgagtc tcatttcctc gcgatcgcga gtatggcgcg
     9841 cagcgacgac gccaggcccg cgcacatcag gagcaggaag ccaaggggca cccatgcgcg
     9901 cacgctccac tgcagggccc aggcg
//
LOCUS       DASPXW010000134         3685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1304206, whole genome shotgun sequence.
ACCESSION   DASPXW010000134 DASPXW010000000
VERSION     DASPXW010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304206"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..2976)
                     /locus_tag="VEL04_03210"
     CDS             complement(112..2976)
                     /locus_tag="VEL04_03210"
                     /inference="COORDINATES: protein motif:HMM:NF015926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HYT47404.1"
                     /translation="MGILEKLRPTPRWKHTDPSVRVAAVYELGGDEGEALHGLAREDA
                     EPRVRRAAVTRIDDVAILGEIARTDPDEDVRAEAVRGLAGVAAEADEVEHAIEASRQL
                     LALGRTKEIVLVARDSSSPAVRAAIIDLIEDPKALGSVSRHAQDSSTRLRALARVHES
                     DEILNVALKSEHTDAAVAALERITDRDALAAIAQRARNKVAARRGRARLRLLEEPAAP
                     AQDESAVRMNADDRQRAVALLHKAEALVAIADPDEAATALAGTRLAWAEMQADVDVEP
                     LLVQQFESASDAVREAIAERRQERAAEEERLQAMLKEQADRVAICEELERLSGGDDVD
                     RVAELKVRWDNLPPMPSDYAASLTRRFQDASRAFEDRERRRLLAQAASARLETLATEL
                     EQLVASEQALEEVIARWRGLGRDADVLREHAPANAAAAERLERAIATLEEKERQQQEI
                     RSTQEQDNLRRLHQVCRQVELLAASEQITLKAGDRALRDIRSALDDRAPVPSKKDRQE
                     IQGRLEAARATLGPRVQELRNADEWQRWANLQVQETLCKEMEALKDEDNLETAGRKMR
                     ELQSRWKQVALAPRAQGEAMWRRFKTAQDEVFNRTAAHFAAQNEERAANLARKLALCE
                     RGEALAGSTDWVKTATEIRALQAEWKTIGPVSRGHEKAVWERFRAACDRFFTRRQEDL
                     KHRKEEWAGNLAKKEALCAQAEALADSTDWDAAASQVKQLQAQWKTIGPVRKSKSDAI
                     WQRFRTACDRFFDRYKHRDQVELQAKAAPRDEVIRALEALLPSDGAEAGPAPENLHAA
                     VQEARTKWQQAPELPRPMQQDLSARYHDLLAKLVRAWPAAFGGTDLDPEGTRRRMEKL
                     LEKVEDLLPENSEPQAALSPAERLAEQWRERLAANTMGGARSAAESEEARWRTAEQEV
                     RNAQAQWARLGPVPADVAGPLNDRFQRACRRFYDLRRRAS"
     gene            3123..>3685
                     /locus_tag="VEL04_03215"
     CDS             3123..>3685
                     /locus_tag="VEL04_03215"
                     /inference="COORDINATES: protein motif:HMM:NF017380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF763 domain-containing protein"
                     /protein_id="HYT47405.1"
                     /translation="MTRLAREIAVYIVADQGPHELLRRLSDPFWFQAFGCVLGFDWHS
                     SGVTTTSTGALKEGIRDIEHDLGVYALGGKGATSRKTPTEILDRCERLAVDPGPLLYA
                     SRMAAKVDSAALQDGYQLYHHSFFFSGSGQWCVVQQGMSDETRTARRYHWLSDSLASF
                     VNEPHEAICCDARSSTLNLVAEEHAPLR"
BASE COUNT          522 a         1281 c         1216 g          666 t
ORIGIN      
        1 gtatgacgcg gcggtacgca gaacgggcgg accgaaaggt ccgcccgaac caagaaccga
       61 acctgaacac gaaccgagaa cagaagaatc agaagcgtga acgatttgtt ctcaggatgc
      121 ccgtcgtctc agatcgtaaa accggcgaca ggcgcgctgg aaccggtcgt tgagcgggcc
      181 cgcgacgtcc gcaggcacgg gacccaggcg cgcccactgc gcctgcgcgt tccgcacttc
      241 ctgctccgcc gtgcgccagc gtgcctcttc gctttcagcc gcgctgcgcg cgccgcccat
      301 cgtgttggcg gcaagacgct cgcgccactg ctcggcgagg cgctcggcgg gcgagagcgc
      361 cgcctgcggc tcgctattct ccgggagcag atcctcgacc ttctccagca gcttctccat
      421 ccgcctgcgc gtcccctcgg gatcgaggtc ggtgccacca aacgcggcgg gccatgcgcg
      481 cacgagcttc gccaggaggt cgtgatagcg cgccgacaga tcctgctgca tcggccgcgg
      541 cagttccggc gcctgctgcc acttcgtgcg cgcctcctgc acggccgcat ggagattctc
      601 gggagcaggg ccggcctcgg ccccgtcgga ggggagcaac gcttcgagcg cgcggatcac
      661 ctcgtcgcgc ggcgccgcct tcgcctgcag ctccacctgg tcgcgatgct tgtagcgatc
      721 gaagaaacgg tcgcatgcgg tgcggaaccg ctgccagatc gcgtcggact tcgacttgcg
      781 gaccggcccg atcgttttcc actgcgcctg cagctgcttg acctgtgagg ccgccgcatc
      841 ccagtcggtt gaatcggcga gcgcctcggc ctgcgcgcag agcgcttcct ttttcgcgag
      901 gttgccggcc cactcttcct tgcggtgctt caggtcttcc tgccgccggg tgaagaacct
      961 gtcgcacgcg gcgcggaagc gctcccacac cgctttctcg tgaccccggc tgacggggcc
     1021 gattgttttc cattccgcct gcagcgcccg gatctcggtg gcggtcttca cccagtcggt
     1081 cgagccggcg agcgcctcgc cacgctcgca gagtgcgagc ttgcgggcga ggttggccgc
     1141 gcgctcttcg ttctgcgcgg caaaatgcgc cgccgtgcga ttgaagacct cgtcctgcgc
     1201 ggtcttgaag cggcgccaca tcgcctcgcc ctgcgcgcgc ggcgcgagcg ccacctgctt
     1261 ccagcgcgac tggagctcgc gcatcttccg gccggcggtc tcgaggttgt cctcgtcctt
     1321 gagcgcctcc atctccttac acagcgtttc ctgcacctgc aggttcgccc agcgctgcca
     1381 ctcgtcggcg ttgcggagtt cctggacgcg cgggccgagc gtcgcgcgcg ccgcctcgag
     1441 acggccctgg atttcctgcc ggtctttttt cgacggcacc ggcgcgcgat cgtcgagcgc
     1501 cgaccggatg tcgcgcagcg cgcggtcgcc ggccttcagc gtgatctgtt cggaggcagc
     1561 cagcagttca acctggcggc agacctggtg gaggcggcgc aggttgtcct gttcctgcgt
     1621 cgagcggatc tcctgctgct gacgctcttt ctcttcgagc gtcgcgatcg cgcgctcgag
     1681 ccgctcggcc gccgcggcgt tggccggtgc gtgctcgcgc agcacatcgg cgtcgcgacc
     1741 caacccgcgc caccgcgcga tcacttcctc gagtgcctgt tctgatgcga ccagctgctc
     1801 gagctccgtc gccagcgtct ccaggcgcgc cgatgcggcc tgcgcgagga ggcgccgccg
     1861 ctcacgatcc tcgaaggcac gcgaggcgtc ctggaagcgg cgggtgagcg acgccgcgta
     1921 atcggacggc atcggcggca ggttgtccca tcgcactttc agttcggcca cgcggtcgac
     1981 gtcgtcgccg cccgacagcc gttcgagctc ttcgcagatc gcgacgcgat cggcctgctc
     2041 cttgagcatc gcctgcagac gttcttcttc cgccgcgcgc tcctgccggc gttcggcaat
     2101 cgcctcgcgc acggcatcgc tcgccgactc gaactgctgc acgagcagcg gctcgacgtc
     2161 gacgtcggcc tgcatctccg cccacgcaag ccgggtcccg gccagcgctg tggcggcttc
     2221 gtcgggatcg gcgatcgcga cgagcgcctc ggccttgtgc agcaacgcga ccgcgcgctg
     2281 gcgatcgtcg gcattcatgc ggacagccga ttcatcctgc gccggcgccg ccggctcttc
     2341 gagcagtcga agcctggcgc gtccgcggcg ggcggccacc ttgttgcgcg cgcgctgcgc
     2401 gatggcggcg agcgcgtcgc gatcggtgat gcgctcgagc gcggcaacgg cggcgtcggt
     2461 gtgctcggac ttcagcgcga cgttgagaat ctcatcggac tcatgcacgc gcgccagcgc
     2521 gcgaaggcgg gtggagctgt cctgcgcatg gcggctcacg gagccaagcg ctttcggatc
     2581 ctcgatcagg tcgatgatgg cggcgcgaac ggcgggcgat gagctgtcgc gggcgacgag
     2641 cacgatctct ttcgtgcgcc cgagcgcaag gagttgccgg gacgcctcga tggcgtgctc
     2701 gacctcgtcg gcctcggcgg ccacgccggc caggccgcgc accgcttccg cgcgcacgtc
     2761 ctcgtcggga tccgttctcg cgatctcgcc caggatggcg acatcgtcga tgcgggtgac
     2821 ggcggcgcgc cgcacgcgcg gctcggcgtc ttcgcgggcg agcccgtgga gcgcctcccc
     2881 ttcgtcgccg ccaagctcat aaaccgccgc gacgcgcacc gacggatccg tgtgtttcca
     2941 gcgaggtgtc ggacgaagct tttcgaggat gcccatcttg tctatcggga aagcgatccg
     3001 gggagcgccg gatcatttaa tctaaggctc cgaaacccgt aagttgttac atgtcccgtc
     3061 acaccggtta cgcccatctg ccgttacatc ctggacgcgc gccagcctgg ctgtttgcgc
     3121 gaatgacccg gctcgccagg gagattgccg tctacatcgt ggccgaccag gggcctcacg
     3181 agctgctcag gcgactgtcc gatccgttct ggttccaggc gttcggatgc gttctcggct
     3241 tcgattggca ttcgagcggt gtgaccacta cgtccaccgg agcgctcaaa gaaggcatcc
     3301 gtgatatcga gcacgacctt ggggtctatg cactaggcgg caagggagcg acatcgcgca
     3361 aaacgccaac cgaaattttg gatcggtgcg aacggttggc cgtagatccg gggccgcttc
     3421 tatatgccag tcgcatggca gccaaggttg acagtgctgc gttgcaggat gggtaccagc
     3481 tctaccacca ttcgttcttt ttttcgggct ccggccaatg gtgcgtcgtc cagcagggaa
     3541 tgagcgacga aacgcgaacg gcgcggcgct atcactggct gtctgattcg ctggccagct
     3601 tcgtcaatga gccgcatgaa gctatctgct gcgatgcccg cagctcgacc ttgaacctgg
     3661 tggcggagga gcatgctccg ctccg
//
LOCUS       DASPXW010000135         2605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_921627, whole genome shotgun sequence.
ACCESSION   DASPXW010000135 DASPXW010000000
VERSION     DASPXW010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921627"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..800
                     /locus_tag="VEL04_03220"
     CDS             144..800
                     /locus_tag="VEL04_03220"
                     /inference="COORDINATES: protein motif:HMM:NF023410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsI family protein"
                     /protein_id="HYT47406.1"
                     /translation="MITRLLVISALFLGSAGYVSHKTHPEKVGTRTPLASLPLRVERW
                     QGHEGAPFAANIVKVLGADEYINRVYVSRQEPYVSLYVGFYQSQRQGDTMHSPLNCLP
                     GAGWQPVRQDRATIAVNDPNGAARAVTVNQFVIEKGLERQMVLYWYQSHGRVIASEYA
                     SKIYLVYDALRLNRTDAAMVRVTTPIAGTAASDERAAAARLIEFVQHMFPQLSALLPQ
                     "
     gene            839..>2605
                     /locus_tag="VEL04_03225"
     CDS             839..>2605
                     /locus_tag="VEL04_03225"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYT47407.1"
                     /translation="MFRTKFYVVALAALTLGMPGCSKDPEVRKRDFVKSGDNYVAQKK
                     LGEAIIEYRNAVGVDPRFGEARLKLAEAYMATGDVQNAYREYVRAADLMPGSIDAQLK
                     TGTLLLLSQQYEDAKGRAEKALAIDPKNVNAQILKGNALAGLKDIDGAIAQVESAIKS
                     DPSQGIPYSSLGALQLAKGNTDLAEKAFKQAVASDPKSVPAFLGLANFYWSTRRNAEA
                     EAALNSALALEPRHVLANRALATFYLFSGRVAEAEQPLKTIAEVMPTAAGKLALADYY
                     LNTKKPAPAKALLEQASTMRDGFAPARLKLAAIAVRDGKRPEAYRLLDEVLTKEPKHV
                     EALAAKAELQIGDGKLDEALIGINTGLSANPNSAPLHLLRGRALAGRNETSDAMAAYR
                     EALRHNPQLLAADIELGRLYLAAGKPSDAIQYAEGALKLAPAFADAQLLLARAYIANR
                     DARSAEEVLKRLNAAYPAAPVVQTEMGRLYLAKGDKVNGRAAFQRALDKDPAQLTALE
                     GLLVLDLTDKPTDARARIEGALAKTPRNDGLYLLAGRTYIALKDSAAAERALKQALEI
                     NPNNLQAYSVLGRYYAIQARIPE"
BASE COUNT          531 a          822 c          836 g          416 t
ORIGIN      
        1 cggaagggtt cttccacgag ttctcaggcc tgatggtgtt cgcggcggca ctcgcaatgc
       61 tggcgctcgt cgcgaagctc gttcaactgg cgtggccgca ggccggtcgc cgccccgaac
      121 ccgcatcggt ggtggtctaa cgcatgatta cgcgcctgct cgtcatcagt gcgctcttcc
      181 ttggaagtgc cgggtacgtc tcacataaga cccatcccga gaaggtcggc acgcgcactc
      241 cgctggcgtc gctgccgctc cgcgtcgagc ggtggcaagg acacgaaggg gcaccgtttg
      301 cggcgaacat cgtgaaagtg ctcggcgccg acgagtacat caacagagtg tacgtgtcac
      361 gccaagagcc atacgtgagc ctgtatgtgg gcttctatca gagccagcgc cagggtgaca
      421 cgatgcattc gccgctcaac tgtctaccgg gcgcgggctg gcagccggtg cgtcaggatc
      481 gtgcgaccat cgccgtgaac gacccaaatg gagccgcgcg agccgtgacc gtgaatcagt
      541 tcgtcatcga gaaggggctc gagcgccaga tggtgctcta ttggtatcag agccacggca
      601 gggtcatcgc cagcgagtac gcgagcaaga tctatctagt atacgacgcc ctgcggctga
      661 accgaaccga cgctgccatg gtacgggtca ccaccccaat cgccggaacg gccgcatccg
      721 acgagcgcgc cgctgccgcg cgcctgatcg agttcgtcca gcacatgttt ccacagttga
      781 gcgcattgct gccgcagtga atgccacagt aattccggaa atacagggag accatcagat
      841 gttccgcacc aagttctacg ttgtcgcact cgccgcgctg acactcggaa tgccggggtg
      901 ttcgaaagac ccggaggtca gaaagcggga cttcgtaaag agcggggaca actacgtcgc
      961 gcagaaaaag ctgggcgagg caatcatcga gtaccggaat gccgtcggcg tcgatccacg
     1021 gttcggcgag gcgcgcctca aactcgccga ggcgtatatg gcaaccggcg acgttcagaa
     1081 tgcctaccgg gaatatgtgc gcgcggccga cctcatgccg ggcagcatcg acgcgcagct
     1141 gaaaacaggg acactgctcc ttctctcgca gcaatacgag gatgcaaagg ggcgcgccga
     1201 aaaggcgctc gcgatcgatc cgaaaaacgt caacgcgcag atcctcaagg gcaacgcgct
     1261 cgccggcctg aaggacatcg acggggccat cgcgcaggtc gagtccgcga tcaagagcga
     1321 cccatcgcaa gggataccgt actccagcct cggcgccctg cagcttgcga aaggaaacac
     1381 cgacctcgct gaaaaggcgt tcaagcaggc cgtcgcgtcc gacccgaaat cggtgccggc
     1441 attcctcggg ctcgcgaact tctactggtc gactcgccgg aatgcggagg cggaggcggc
     1501 gctcaactcg gcgctcgcgc tcgaaccccg tcacgtgctg gcaaaccggg cactggcgac
     1561 gttctatctt ttttccggcc gcgtcgccga agcagaacag ccgttgaaga cgatcgcgga
     1621 ggtgatgccg acggcggcgg gcaaactcgc cttggcggac tactatctca acacgaagaa
     1681 accggcaccg gccaaggcgc tgctggagca ggcctcgacg atgcgtgacg gtttcgcccc
     1741 tgccaggctg aagctcgcgg cgatcgcggt gagagacggc aagcgccctg aggcgtaccg
     1801 gctgctcgac gaggtgttga cgaaggagcc gaagcacgta gaggcgttgg cggccaaagc
     1861 ggagctgcaa atcggcgacg ggaaactcga cgaggcattg atcggcatca acaccggtct
     1921 gtcggcgaac ccgaattctg ctccgctcca cctgcttcgg gggcgcgcac ttgcggggcg
     1981 caacgagacg agcgacgcga tggcggctta cagggaggcg ctccgtcaca atccgcagct
     2041 tctggcagcc gacatcgagc tcggccgtct ctatctcgcc gcggggaagc cgtccgacgc
     2101 gatccagtac gcggagggcg cgctcaagct ggccccggct ttcgccgacg cgcagttgct
     2161 gcttgcgcgc gcctacatcg cgaaccgcga tgcgcgcagc gccgaagagg ttctcaagag
     2221 attgaacgcc gcatatccgg ctgcgccagt cgtgcagacg gagatggggc gcttgtacct
     2281 cgcgaagggc gataaagtga acggccgcgc cgcgtttcag cgcgcgctcg acaaggatcc
     2341 ggcgcagctc acagcgctcg aagggctcct cgtcctcgac ctgacggaca aaccgaccga
     2401 tgcgcgcgcg cggatcgaag gtgcccttgc gaagaccccg cggaatgatg gactgtacct
     2461 gctcgccggc cggacgtata tcgctttaaa ggattccgcg gcggcggaac gcgcgttgaa
     2521 acaggcgctc gaaatcaacc cgaacaacct gcaggcttat tcggtgctcg gccgctacta
     2581 tgcgatccag gcacggatcc ctgaa
//
LOCUS       DASPXW010000136         2772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_670535, whole genome shotgun sequence.
ACCESSION   DASPXW010000136 DASPXW010000000
VERSION     DASPXW010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670535"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /gene="thiC"
                     /locus_tag="VEL04_03230"
     CDS             complement(<1..1089)
                     /gene="thiC"
                     /locus_tag="VEL04_03230"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HYT47408.1"
                     /translation="MNANPKFLAATAHVDDAAMQPLPNSRKVYLGSLRVPMREITQSD
                     GNPLIHVYDTSGPYTDPEAKIDIRKGLAPLREAWISQRNDCQILAAPSSEYGRKRLAD
                     PKLAELRFDLQRRPRRASKGNNVSQMHYARRGIITPEMEYIALRENQRMVDSLGFQHP
                     GQSFGASLPKVVTPEFVRDEVARGRAIIPANINHPETEPMIIGRNFLVKINCNIGNSA
                     VTSSIGEEVDKMTWAIRWGADTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEK
                     TGGKAEDLTWEIFRDTLIEQAEQGVDYFTIHAGVRLPFIPLTAGRMTGIVSRGGSILA
                     KWCITHHRENFLYTHFDEICDIMKAYDVS"
     regulatory      complement(1080..1179)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1203..1856
                     /locus_tag="VEL04_03235"
     CDS             1203..1856
                     /locus_tag="VEL04_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HYT47409.1"
                     /translation="MNLDQARANMVEQQIRTWEVLDQDVLDLLYLVPREEFVPGQHRA
                     LAFSDTELPIGEGEKMWAPKMEARVLQELNARKSDRALEIGTGSGYLTALLAHRSAHV
                     HSVEINPALGAFGRRNLERHGADHVTLEAGDAARGWPRHAPYDIIVLTGSTPVLPASF
                     LEQLAPEGRLFAVVGEAPAMTARLVTCTGPGAYRTTDLFETVIKPLVHCARPSGFRF"
     gene            1934..>2772
                     /locus_tag="VEL04_03240"
     CDS             1934..>2772
                     /locus_tag="VEL04_03240"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HYT47410.1"
                     /translation="MRGLTPVFVLLAALPLAAAAEDLVQVYRDAQRYDAVYSAARLAL
                     EAGREKLPQGRALVLPTLNLSGSATRSRFDVESKDPAVSPSFVRQPNSAAYTLTLTQP
                     LYRPQNLIQYNQGEYQVKQAEAIFGNASQDLVIRVAQAYFDVLAAQDSLGLVRAQKAA
                     ISEQLAQAKRNFEVGTATITDTHEAQARYDLSVAQEIAAQNDLENKQRTLQLVTGKLY
                     GELKPLRPDVRLAQPDPNDMQAWVQLAEKQSYPVLVQEATTEIASLEARRNSAAHRPT
                     LDIV"
BASE COUNT          464 a          940 c          889 g          479 t
ORIGIN      
        1 gctcacgtcg taggccttca tgatgtcgca gatctcgtcg aagtgcgtgt agaggaagtt
       61 ctcccggtgg tgcgtgatgc accacttggc caggatcgag ccgccgcgcg agacgattcc
      121 cgtcatgcgc ccggcggtga gcgggatgaa gggcagccgc acgccggcgt ggatggtgaa
      181 gtagtcgacg ccctgctcgg cctgctcgat cagcgtgtcg cggaaaattt cccaggtaag
      241 gtcctccgcc ttgcctcctg ttttttccaa tgcttgataa atcggcaccg tgccgatcgg
      301 caccgggctg ttgcggatga tccactcgcg cgtttcgtga atatgcttgc cggtcgagag
      361 gtccatcacg gtgtccgcgc cccagcggat cgcccaggtc atcttgtcga cttcctcgcc
      421 gatcgaggaa gtgacggcgg aattgccgat gttgcagttg atcttcacca ggaaattgcg
      481 gccgatgatc atcggctcgg tttccgggtg gttgatgttc gccgggatga tggcacggcc
      541 gcgcgccacc tcgtcgcgca cgaattccgg ggtcacgacc ttcggcaggc tggcaccgaa
      601 tgactgtccg ggatgttgga acccaagtga atcgaccatg cgctgattct cgcgcagcgc
      661 gatgtattcc atctcggggg taatgatccc cctgcgcgcg tagtgcatct gggagacgtt
      721 gtttcccttc gaagcacggc gaggcctgcg ctgcaggtcg aagcgcagct cggcgagctt
      781 cgggtcggcg agccttttgc ggccgtactc cgacgaaggc gcggccagga tttggcaatc
      841 atttctttgt gaaatccatg cctcgcgcaa aggcgccaaa cccttgcgga tgtcgatctt
      901 cgcctcgggg tcggtgtagg gcccggaggt gtcgtacaca tggataagcg gattgccgtc
      961 gctttgcgtg atctcgcgca tcggcacgcg cagactgccc aggtagacct tgcgcgagtt
     1021 gggcagcggc tgcatcgccg cgtcgtcgac gtgcgcggtg gcggcgagaa acttcggatt
     1081 ggcgttcatc tgcgctttcc ctccggcggc attacccgca tcaggttata agggtttatc
     1141 tcacccacac cgcccggtgc aggaccccca acgctaaggc tgcgattata atccgcctcc
     1201 tcatgaatct cgatcaagca cgcgccaaca tggtcgagca gcagatccgc acctgggagg
     1261 tgctcgacca ggacgtgctc gacctgctgt acctggtgcc gcgcgaggaa ttcgtgcccg
     1321 ggcagcaccg cgcgctcgcg ttttccgata ccgagctgcc gatcggcgaa ggcgagaaga
     1381 tgtgggcgcc gaaaatggag gcccgcgtgt tgcaggagct gaacgcgagg aagtcggacc
     1441 gcgcgctcga aatcggcacc ggcagcggct acctcaccgc gctcctcgcg caccgcagcg
     1501 cccacgtgca ctcggtcgag atcaaccccg cgctcggcgc cttcggccgg cgcaacctcg
     1561 agcgccacgg cgccgaccac gtcaccctcg aggccggcga cgccgcgcgc ggctggccgc
     1621 ggcacgcgcc ctacgacatc atcgtcctca ccggatcgac cccggtgctg cctgcgagct
     1681 tcctcgagca gctcgcgccc gaagggcggc tgttcgccgt cgtcggcgag gcgccggcga
     1741 tgaccgcgcg cctcgtcacc tgcaccgggc ccggcgccta ccgcaccacg gatctgttcg
     1801 aaaccgtgat caagccgctg gtgcattgcg cgcggccctc gggcttccgc ttctagccgg
     1861 ccgatgctgc agatcacgcc gccgcgatga aacgcaggac ctcgtcgcct tctgccacca
     1921 cggcggccgc agcatgcggg gcctgacccc ggtttttgtt ctgctcgccg cgctgccgct
     1981 tgccgcggcc gccgaagacc tggtccaggt ctatcgcgat gctcagcgtt acgatgccgt
     2041 gtacagcgcg gcgcgcctgg cgctcgaggc aggccgtgag aagctgccgc agggccgcgc
     2101 gctggtgctg ccgacgctca atctttccgg cagcgcgacg cgctcgcgct tcgacgtgga
     2161 atcgaaggac cccgcagtct cgcccagctt cgtgcgccag cccaactcgg ccgcctacac
     2221 cctcaccctg acgcagccgc tctatcggcc gcagaacctg atccagtaca accagggcga
     2281 gtaccaggtg aaacaggccg aggcgatctt cggcaacgcc tcccaggacc tcgtcatccg
     2341 cgtcgcgcag gcctacttcg acgtgctcgc ggcgcaggac tcgctgggcc tggtgcgcgc
     2401 gcaaaaggcg gcgatctccg agcagctggc gcaggcgaag cgcaacttcg aggtcggcac
     2461 cgcgaccatc accgataccc acgaggcgca ggcgcgctac gacctgagcg tggcgcagga
     2521 gatcgcggcg cagaacgacc tcgagaacaa gcagcgcacg ctgcagctcg tgaccggcaa
     2581 gctatacggc gagctcaagc cgctgcgtcc cgacgtgcgg ctcgcccagc ccgatccgaa
     2641 cgacatgcag gcctgggtgc agctcgccga gaagcagagc tacccggtgc tggtccagga
     2701 ggcaaccacc gagatcgcgt ccctcgaagc gaggcgcaac agtgccgcgc accggccgac
     2761 cctcgatatc gt
//
LOCUS       DASPXW010000137         2236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_42042, whole genome shotgun sequence.
ACCESSION   DASPXW010000137 DASPXW010000000
VERSION     DASPXW010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42042"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VEL04_03245"
     CDS             <1..240
                     /locus_tag="VEL04_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47411.1"
                     /translation="EGAPVQVLVVAPPGQREAFEQALLSDARLVFRTIDYEEALQAVK
                     LRRAPEATRAEALFLHLAANNAPREQFASSQDRRR"
     gene            280..894
                     /locus_tag="VEL04_03250"
     CDS             280..894
                     /locus_tag="VEL04_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47412.1"
                     /translation="MLGFAACAIYGGSKWLEVMDLRERAAMQNREARLAAEQYERITA
                     AFPVTQTSTENLKVAVVEFRRIAERSASPERAFVHVSRVLEQFPQMELETLNWSIGRA
                     GERGAMRSPAPAGARAKPEPQGDFAVIVELSGRVNATQRNDYRGITAQVESFAAALST
                     SGYQLIDRKLPFDITSEGTLTGDIGGADTGDAPRFTIVLARNLP"
     gene            891..1499
                     /locus_tag="VEL04_03255"
     CDS             891..1499
                     /locus_tag="VEL04_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47413.1"
                     /translation="MTVTREELRKLALPLLAAVALLAAGAAFIVSAGEALRSANARSA
                     VAQAERRQNAERLARIAEEEREVSEKLDVYQRLKQLNILGEERRLEWADAINRIRVQR
                     ELLDVRYRVERQKLLVSAPGKPGNVDFFASSMRVDLELLHEEDLLRFLADLRDSGNAY
                     YSVRKCTVSRTGQAVGGATMTPRLHAACDIDLVTIIDRAAKR"
     gene            1496..1936
                     /locus_tag="VEL04_03260"
     CDS             1496..1936
                     /locus_tag="VEL04_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47414.1"
                     /translation="MTRALCLLLLALGLGGISGACNAQQLGRLFFTPEQRAALDARRK
                     ARVPDKPAAAAVASPTTRLDGYVKRSSGPSTVWVNGESLLETAPEAPRIGVTRTNDGR
                     VSVTVGESGARVGLKPGEILDRGTGEVRDVIGDGEIRVRPSTPR"
     gene            1933..>2236
                     /locus_tag="VEL04_03265"
     CDS             1933..>2236
                     /locus_tag="VEL04_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47415.1"
                     /translation="MKTASLGRQRGAVLLAMLAVLVLGGMWYLVSRLSAASGDFTAAT
                     RMRNALVLNQAKQALIGYVAAQAAKSAEPRPGALPCPEAAGTDGQAAGNCSLPKVGR"
BASE COUNT          352 a          806 c          762 g          316 t
ORIGIN      
        1 gaaggcgcgc cggtccaggt gctggtggtc gcgcctcccg gccagcgcga ggcgttcgag
       61 caggcgcttc tgtccgacgc tcggctggtg ttccgcacca tcgactacga ggaggcgctg
      121 caggcagtca agctgcgccg cgcgcccgaa gccacccgcg ccgaagcgct gttcctgcat
      181 ctcgccgcca acaacgcgcc gcgcgagcag ttcgccagca gccaggaccg gcgccgctaa
      241 tacatctggc ggctgcagcg cggcatcatc gccgccggcg tgctcggctt cgccgcttgt
      301 gcgatctatg gcgggtcgaa gtggctcgaa gtgatggacc tgcgcgaacg cgctgccatg
      361 cagaaccgcg aggcgcgcct cgccgccgag caatacgaga ggatcaccgc ggctttcccg
      421 gtcacgcaga cctccacgga gaacctgaag gtggcggtgg tcgagttccg gcgcatcgcc
      481 gagcgcagcg cctcgcccga gcgcgctttc gtccacgtgt cccgggtgct cgagcagttt
      541 ccgcagatgg agctcgaaac gctcaactgg agcatcggcc gcgccggcga gcggggcgcc
      601 atgcgctcgc cggccccggc aggcgccagg gcgaaacccg agccgcaagg cgatttcgcc
      661 gtcatcgtcg agctctccgg ccgcgtcaat gccacccagc gcaacgatta ccggggtatc
      721 acggcgcagg tggaaagctt cgccgccgcg ctgtccacct ccggctacca gctgatcgac
      781 cggaagctgc cgttcgacat cacttccgaa ggcacgctca ccggcgacat cggcggcgcc
      841 gacaccggcg acgcgccgcg cttcaccatc gtgctcgccc ggaacctgcc atgaccgtga
      901 cccgcgaaga gctgaggaag ctcgcgctgc ccttgctcgc ggcagtcgcg ctgctggcag
      961 ccggcgccgc tttcatcgtc agcgcgggcg aggcgctgcg cagcgcaaac gcgcgctccg
     1021 ccgtggcgca ggccgagcgc aggcagaacg cggagcgcct cgcgcgcatc gccgaggaag
     1081 agcgcgaagt gagcgaaaag ctcgacgtct accagcgcct caagcagctc aacatcctcg
     1141 gcgaggagcg gcgcctggag tgggccgacg ccatcaaccg catccgcgtg cagcgcgagc
     1201 tgctcgatgt gcgctatcgc gtcgagcggc agaagctgct ggtctccgcg ccgggcaagc
     1261 cgggcaacgt cgatttcttc gccagcagca tgcgggtcga cctggagctg cttcacgaag
     1321 aggatctgct gcgcttcctc gccgatctca gggattcggg caacgcctat tactcggtga
     1381 ggaaatgcac ggtgtcgcgc accggccagg cggtgggcgg cgccaccatg accccgcggc
     1441 tgcacgccgc gtgcgacatc gacctcgtca ccatcatcga ccgggcggcg aagcgatgac
     1501 acgcgccctt tgtctgctgc tcctcgccct tggtctggga ggaatttctg gtgcctgcaa
     1561 cgcccagcag ctcgggcgcc tgttcttcac gccggagcag cgcgcagcgc tcgatgcgcg
     1621 ccgcaaggcg cgcgtgccgg acaagcctgc ggccgcggcc gttgcctcgc ccaccacgcg
     1681 cctcgacggc tatgtgaagc gctcgagcgg accctcgacg gtgtgggtga acggcgagtc
     1741 gctgctcgag accgcgcccg aagcaccgcg tatcggggtc acgcgcacca acgacggccg
     1801 cgtctccgtg accgtcggcg agagcggcgc gcgcgtcggc ctgaagcccg gcgagatcct
     1861 cgaccgcggc accggcgaag tgcgcgacgt catcggcgac ggcgagatcc gcgtgcgtcc
     1921 gagcacgccg cgatgaaaac cgcttccctg gggcggcagc gcggcgcggt gctcctggca
     1981 atgctcgcag tgctggtgct cggcggcatg tggtacctgg tctcgcggct cagcgcggca
     2041 agcggcgatt tcacggccgc cacgcgcatg cgcaatgccc tcgtcttgaa tcaagcgaag
     2101 caggccctca tcggctacgt ggccgcgcag gccgccaaat ccgcggagcc ccgccccgga
     2161 gccctgcctt gccccgaagc cgcaggtacc gacggccagg ccgccgggaa ctgctcgctg
     2221 ccgaaagtcg ggcgtt
//
LOCUS       DASPXW010000138         2212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1050929, whole genome shotgun sequence.
ACCESSION   DASPXW010000138 DASPXW010000000
VERSION     DASPXW010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050929"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VEL04_03270"
     CDS             <1..259
                     /locus_tag="VEL04_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47416.1"
                     /translation="EGEPRALAGPSTPAPPSAGGPMAPAPPPAGGPLAPAPPPAGGPM
                     GTRRGGGLSPEYQARIAALEADVQAARVRNQEAWARFNRLR"
     gene            complement(291..1085)
                     /locus_tag="VEL04_03275"
     CDS             complement(291..1085)
                     /locus_tag="VEL04_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine/phosphatidylserine synthase"
                     /protein_id="HYT47417.1"
                     /translation="MGEDPEFESEVTTPTEPGRLGGLRRRGIYLLPNLFTTGVLFCGF
                     FAIVQAMNGRFEVGAIAIYVAMVLDGMDGRVARWTNTQSEFGAQYDSIADMVAFGAAP
                     ALMAYVWVLRDLGTAGWIGAFIYCAGAGIRLARFNANIAVVDKRYFQGLPSPSGAAVM
                     AGLLWVLVDFGFTPVEHLWIAGVTWVVAVFVGVTMVSNVPFWSFKEVNWKKRVPLWVI
                     LACVVAISIVASRPSLILFALFMSYALSGYVMWGMGRRVRPVLPEE"
     gene            complement(1052..1720)
                     /locus_tag="VEL04_03280"
     CDS             complement(1052..1720)
                     /locus_tag="VEL04_03280"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HYT47418.1"
                     /translation="MRPPYPHPLLAREGWPFVGTGIAFSLGICLFLPWWWSLPFWLAT
                     LFVVQFFRDPPREVPDDARAVVSPADGRVVEVGRSRDPWLERDALKVSVFMNVFNVHS
                     NRSPVDGVVRERWYFPGAFLNAALDKASLENERNALWLRTRDGHDVTCVQVAGLIARR
                     ILCYVGAGAELVRGQRFGFIRFGSRVDVYLPLDAEIKASLGDKVYAAQSVLAWVKTPN
                     SKAK"
     gene            complement(1724..1885)
                     /locus_tag="VEL04_03285"
     CDS             complement(1724..1885)
                     /locus_tag="VEL04_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47419.1"
                     /translation="MLLAACGDKVPESEAARRIGQQPKKTVDSVSSKVGNLMQQGQGS
                     ERLKEEEQK"
     gene            complement(1902..>2212)
                     /locus_tag="VEL04_03290"
     CDS             complement(1902..>2212)
                     /locus_tag="VEL04_03290"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902902.1"
                     /note="catalyzes the formation of
                     (R)-2,3-dihydroxy-3-methylbutanoate from
                     (S)-2-hydroxy-2-methyl-3-oxobutanoate in valine and
                     isoleucine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HYT47420.1"
                     /translation="LMYEGGIGTMNYSISNNAEYGEYTSGPKIIDEDTRKRMRAVLAR
                     IQSGEYAKEFVLENRAGAPTLLSRRRMTAELQIEKVGARLREMMPWIRRNRLVDTSKN
                     "
BASE COUNT          381 a          788 c          717 g          326 t
ORIGIN      
        1 ggaaggcgag ccgcgcgccc ttgccggtcc gtcgactccg gcgccgccct ccgcgggggg
       61 accgatggcg cccgctccac cgccggcggg gggaccgctg gcgcccgccc ctccgccggc
      121 gggcggcccg atgggcacgc ggcgtggcgg cggcttgagt cccgaatacc aggcgcgaat
      181 tgcagcgctc gaggcggacg tccaggcggc gcgcgtgcgc aaccaggagg cctgggcgcg
      241 cttcaaccgg ctgcgttgat cgcgggcgcc gcccggcacg cgctgccttt tcactcctcg
      301 ggcaacaccg gtcgcacgcg ccttcccatg ccccacatca catagcccga cagcgcgtac
      361 gacatgaaca gcgcgaacag gatcagcgac ggccgcgaag cgacgatgga gatggcgacc
      421 acgcaggcga ggatcaccca gagcggcacg cgcttcttcc agttcacttc cttgaagctc
      481 cagaagggca cgttcgatac catggtgacg ccgacgaaca cggcgacgac ccaggtcacg
      541 ccggcgatcc acagatgctc gaccggcgtg aagccgaaat cgaccagcac ccatagcagg
      601 ccggccatca ctgccgcgcc cgagggactc ggcagcccct ggaagtagcg cttgtcgacc
      661 acggcgatgt tggcgttgaa gcgcgcgagg cggatgccgg cgccggcgca gtagatgaac
      721 gcgccgatcc atcccgcggt gccgaggtcc ctcagcaccc acacgtaggc catgagcgcg
      781 ggcgcggcgc cgaacgccac catgtcggca atgctgtcgt actgcgcgcc gaactcgctc
      841 tgcgtgttgg tccagcgcgc cacgcggccg tccatgccgt cgagcaccat cgccacgtag
      901 atcgcgatcg cgccgacctc gaagcggccg ttcatcgcct gcacgatggc gaaaaagccg
      961 cagaacagca cgcccgtcgt gaacaggttc ggcagcaggt agatgccgcg ccgccgcagg
     1021 ccgcccaggc ggccgggctc ggtcggcgtg gttacttcgc tttcgaattc ggggtcttca
     1081 cccatgcgag cacgctctgc gctgcgtaca ccttgtcccc gagcgacgcc ttgatttccg
     1141 catcgagcgg cagatagacg tcgacccggg agccgaagcg gatgaagccg aagcgctgtc
     1201 cgcgcaccag ctccgcgccg gcgccaacat agcacaggat gcgccgggcg atcagccccg
     1261 cgacctgcac gcaggtgaca tcgtgcccgt cgcgcgtgcg cagccacagc gcgttgcgct
     1321 cgttctcgag cgatgccttg tcgagcgcgg cgttgaggaa ggcgccggga aaataccagc
     1381 gctccctgac cacgccgtcc accggcgagc ggttcgaatg cacgttgaac acgttcatga
     1441 acacgctcac cttcagcgcg tcgcgctcga gccagggatc gcgcgacctg cccacctcga
     1501 ccacgcggcc gtccgcgggc gacaccacgg cgcgcgcatc gtcgggcacc tcgcgcggcg
     1561 gatcgcggaa gaactgcacc acgaaaaggg tcgccagcca gaaggggagc gaccaccacc
     1621 agggcagaaa aaggcagatt cccagtgaga aagcaatccc tgtgccgacg aacggccagc
     1681 cttcacgcgc gagcaacggg tgcgggtacg gcggcctcac gagctatttc tgctcttctt
     1741 ccttgagccg ctccgagccc tgcccctgct gcatcagatt gccgaccttc gaagaaacgc
     1801 tgtccacggt ctttttcggc tgctggccga tgcgccgggc ggcctcggac tccggcacct
     1861 tgtcgccgca ggcggcgagc agcaacagcg ccgtgaaaaa gctagttctt cgaagtgtcg
     1921 acaaggcggt tcctcctgat ccagggcatc atttcgcgca gccgggcgcc gaccttctcg
     1981 atctgcagct cggcagtcat gcgccggcgc gacagcagcg tcggcgcgcc ggcgcggttc
     2041 tcgagcacga actccttcgc gtactcgccc gactggatgc gcgccagcac cgcgcgcatg
     2101 cgcttgcgcg tgtcctcgtc gatgatcttc ggtcccgagg tgtattcgcc gtactcggcg
     2161 ttgttcgaga tcgagtagtt catggtgccg atgccgccct cgtacatcag gt
//
LOCUS       DASPXW010000139         4299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1051085, whole genome shotgun sequence.
ACCESSION   DASPXW010000139 DASPXW010000000
VERSION     DASPXW010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051085"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="VEL04_03295"
     CDS             complement(<1..1008)
                     /locus_tag="VEL04_03295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYT47421.1"
                     /translation="MRVAFVLKGYPRLSEAFIAQEIAALERRGLGILIVALRRQTDTR
                     VHAIHAGIRAAVTYLPEYLLLEPFRVLRAWWRTRKLETYSKTFALWLRDLIRDPTPNR
                     IRRFGQALVLAAELPRDVARLHAHFLHTPASVTRYAALLLGLPWSGSAHAKDIWTTPE
                     WEKREKLASCDWLVTCTKVNRDHLAALAPAGRVELVYHGVDLARFPRHSSSREMKDGT
                     DARDPVIILSVARLVEKKGTDVLLEALARLPKDLNWKFVHVGGGPLRGKLERRARALG
                     IARRVEWRGPLTQDRLLEAYRKADLFALASRIARDGDRDGLPNVLAEALSQGLACVAT
                     RV"
     gene            complement(1009..2232)
                     /locus_tag="VEL04_03300"
     CDS             complement(1009..2232)
                     /locus_tag="VEL04_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47422.1"
                     /translation="MRSRRNDGRVLIYSHDTFGLGHLRRCRALAHALVDRFKQLSVLI
                     LSGSPIIGSFDFRARVDFVRVPGVIKLRNGEYTSLNLHLDISETLSMRASIIKHTADI
                     FAPDLFIVDKEPVGLRGEVLETLKMLKRRGTRLVLGLRDVMDEPRLLEPEWQRKKVLP
                     ALRELYDEIWVYGLPQICDPLEGIGIPQRVRQKMVYTGYLHREVPTHGAPPRMPEMVQ
                     RPYVLVTTGGGGDGEGLIDWVLRAYEHDPLLPYPALLVLGPFMQPERQAEFMDRAARL
                     KRVDAITFHGQLEALVSRAAAVVAMGGYNTFCEVLSLDKRTLIVPRTAPRLEQHIRAS
                     RAAELGLVTMLSDDGGYDPAVMAAALRALPRQGRPSEVVVPGLLEGLPNVSRLVAPWI
                     EPAAVEAPQALSRIG"
     gene            complement(2290..2877)
                     /locus_tag="VEL04_03305"
     CDS             complement(2290..2877)
                     /locus_tag="VEL04_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47423.1"
                     /translation="MTTLVMFSGGLDSTAMLVKLLEQTADELRVHHIRMVNREGRAGA
                     EQAAVERIVAHCRPRYRPFRYSESGLDFAALEAIPIDYLSIAFVACQVAIDTPGCTRV
                     AVAALARDTDIANRLARQRRVFETLYECYRARKLGEPQVEWVTPVYDSSKHELAAALP
                     AELAALAWSCRRPVASAGGWRPCGGCKACLARQAA"
     gene            2906..3682
                     /locus_tag="VEL04_03310"
     CDS             2906..3682
                     /locus_tag="VEL04_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYT47424.1"
                     /translation="MKVRDLLDLSGRVALVTGGSRGLGLQIAEALGEMGAKLALTARK
                     KDELEQAKKHLASLGVSAQAFVCDLGKRETIPPVVDQVLGTFGKVDILVNNAGATWGA
                     PAEDHPLEAWDKLVNLNLTGAFVITQLVGGKSMIPARWGRIINIASVAGLMGQDPRIV
                     RTIAYNATKGGLVNFTRALAAEWGSHGITVNAICPGFFPSKMTRATLGTTGELIRDWT
                     PTKRLGNDEDLKGLAVLLASEASRHITGQAIAVDGGATII"
     gene            3697..4116
                     /locus_tag="VEL04_03315"
     CDS             3697..4116
                     /locus_tag="VEL04_03315"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HYT47425.1"
                     /translation="MSDRLAFTRDYQRKIPFVSHLKILTETLGEGTARLSLPIELHLT
                     NSLGTAHGGVIVSLLDVALCTAARTLHPESMGVVTIDLSTSFIGGASGSKLIAEARVL
                     RDGRSMSFVEAEAKNEDGSLVAKAIATVRVRHKKDAG"
     gene            4113..>4299
                     /locus_tag="VEL04_03320"
     CDS             4113..>4299
                     /locus_tag="VEL04_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYT47426.1"
                     /translation="MRSPTRGVHHLALTTEDMKLTTEFYVGVVGMPLVHAMKVPPGLG
                     SGPGNRGNPPYEEIRHYF"
BASE COUNT          736 a         1448 c         1473 g          642 t
ORIGIN      
        1 cacgcgtgtc gccacgcagg cgagcccctg gctcagtgcc tcggcaagca cgttgggcag
       61 gccatcgcgg tcgccgtcgc gcgcgatgcg gctcgctagc gcgaacaggt ccgccttcct
      121 gtatgcctcg agcaacctgt cctgcgtaag cggaccgcgc cactcgacgc gccgagcgat
      181 gccgagggcg cgcgcgcggc gctcgagctt gcctctcagc ggcccgccgc ccacgtgtac
      241 gaatttccaa ttgagatcct tgggaaggcg cgcgagcgcc tcgagcagca cgtcggtgcc
      301 tttcttttcg accagccgtg ccacggacag aatgatcacc ggatcgcgcg catccgtgcc
      361 atctttcatt tccctggaag aggaatggcg gggaaagcgc gcgagatcga cgccgtgata
      421 gacgagctcc acgcgtccgg cgggtgcaag cgcggcgaga tggtcgcggt tgaccttggt
      481 gcaggtgacg agccagtcgc acgaggcgag cttctcgcgc ttctcccatt ccggggtggt
      541 ccagatgtcc ttggcatgcg ccgagccgct ccacggcagc ccgagcagga gggcggcgta
      601 gcgcgtcacc gacgcgggcg tgtgcaggaa atgcgcgtgc agccgcgcga cgtcgcgcgg
      661 cagctcggcg gcgagcacca gcgcctgccc gaagcggcgg atgcgattag gagtcgggtc
      721 gcgaatgagg tcgcgcagcc acagcgcaaa ggtttttgaa taggtctcga gttttcttgt
      781 acgccaccag gcccgcagaa cccggaacgg ttccaaaagc agatactcgg gaaggtaggt
      841 gaccgcggcg cggatcccgg cgtggatcgc gtggacacgc gtgtcggtct ggcggcgcag
      901 cgcaacgatc aggatgccga ggccgcggcg ctcgagcgcc gcgatctcct gggcgatgaa
      961 cgcctcggag agacgcggat agcccttcag tacaaaagca actctcaact aaccgatgcg
     1021 cgagagagct tgcggcgcct cgaccgccgc cggctcgatc cagggcgcga ccagccgcga
     1081 cacgttcggc aggccttcga gcagcccggg caccactacc tccgaaggcc ggccctgccg
     1141 cggcagcgcg cgcagcgctg cggccatcac tgcggggtcg tagccgccat cgtcgctcag
     1201 catggtgacg aggcccagct cggccgcgcg cgaggcgcgg atgtgctgct cgagacgggg
     1261 cgcggtgcga ggcacgatga gcgtgcgctt gtcgagcgac agcacctcgc agaaagtgtt
     1321 gtagccgccc atcgcgacca ccgccgcggc gcgcgacacc agcgcctcga gctggccgtg
     1381 gaaggtgatc gcgtcgactc gcttcaggcg cgcggcgcgg tccatgaact cggcctggcg
     1441 ctcgggctgc atgaacggcc cgagcacgag cagcgccgga taaggcagta gcgggtcgtg
     1501 ctcgtaggcg cgcagcaccc agtcgatcag tccttcgccg tcgccgccgc cgccggtggt
     1561 gacgagcacg tacggccgct gcaccatctc gggcatgcgc ggcggcgcgc cgtgggtcgg
     1621 cacctcgcgg tgcaggtagc cggtgtagac catcttctgc cgcacgcgct gcgggatgcc
     1681 gatgccctcg agcggatcgc agatctgcgg caggccgtag acccagatct cgtcgtacag
     1741 ctcgcgcagc gccggcagca ctttctttct ctgccattcg ggctcgagca ggcgcggctc
     1801 gtccatcacg tcgcgcaggc cgagcaccag ccgggtgccg cggcgcttca gcatcttcag
     1861 cgtctcgagc acctcgccgc gcagcccgac cggctccttg tcgacgatga acagatccgg
     1921 cgcgaagata tccgcggtgt gcttgatgat cgaggcgcgc atcgacagcg tctcgctgat
     1981 gtcgaggtgc aggttgagcg aggtgtactc gccgttcctg agcttgatca cgccgggcac
     2041 gcgcacgaaa tcgacgcgcg cgcggaaatc gaagctgccg atgatcggcg agcccgacag
     2101 gatcaggacc gagagctgct tgaagcggtc gaccagcgcg tgcgcgagag cgcggcagcg
     2161 cctcaggtgg ccgaggccga aggtgtcgtg gctgtagatg agtacgcggc catcgttccg
     2221 cctggacctc atggcgcccc ctcccctttg cagcccggat tgtgggccgg gaaaaaggaa
     2281 atacgcaagt caggcggcct gccgcgcgag gcaggctttg catcccccgc agggccgcca
     2341 ccccccggcg ctggcgaccg ggcggcggca cgaccaggcc agggcggcga gctccgcggg
     2401 caaggccgcg gcgagctcgt gcttgctcga gtcgtacacc ggagtgaccc actcgacctg
     2461 cggctcgccg agcttgcgcg cgcggtagca ctcgtacagc gtctcgaaca cccggcgctg
     2521 gcgtgcgagg cgattcgcga tgtcggtgtc gcgcgcgagc gccgcgaccg cgacgcgcgt
     2581 gcagccgggc gtgtcgatcg cgacctggca ggcgacgaag gcgatcgaaa ggtaatcgat
     2641 cgggatcgcc tccagcgccg cgaaatccag gcccgactcg gaatagcgga acggccggta
     2701 gcgcggcctg cagtgcgcca cgatgcgctc gaccgccgcc tgctccgcgc cggcgcggcc
     2761 ttccctgttg accatccgga tgtggtgcac gcgcagctca tcggccgtct gctcgagcag
     2821 cttcaccagc atcgcggtgc tgtccagtcc acccgagaac atgacaagcg tggtcatccg
     2881 gcaattctag ctttacgatt gcgttatgaa ggtcagagac ctgctggacc tgagcggacg
     2941 cgtcgcgctg gtgacgggcg gctcgcgcgg cctcgggctg cagatcgccg aagcgcttgg
     3001 cgagatgggc gcgaagctcg ccctcacggc gcggaaaaag gatgagctgg agcaggcgaa
     3061 aaaacatctc gcctcactgg gtgtttctgc tcaggctttc gtctgtgatc tcggcaagcg
     3121 cgagaccatc ccgccagtgg tcgaccaggt gctcgggaca ttcggcaagg tcgacatcct
     3181 ggtgaacaac gccggagcca cctggggagc gccggccgag gaccatccgc tggaagcctg
     3241 ggacaagctc gtcaacctca acctgaccgg ggctttcgtc atcacgcagc tcgtcggagg
     3301 caaatcgatg atcccggcgc gctggggccg catcatcaac atcgcctcgg tcgccggcct
     3361 gatgggccag gatccgcgca tcgtgcgcac catcgcgtac aacgcgacca aaggcggcct
     3421 ggtgaacttc acgcgcgcgc tcgccgccga atgggggagc cacggcatca cggtgaacgc
     3481 gatctgcccg ggcttctttc cctcgaagat gacgcgcgcg acgctcggca ccaccggcga
     3541 gctgatccgc gactggacgc cgaccaagcg cctgggaaac gacgaggacc tgaagggcct
     3601 cgcagtgctg ctcgcttccg aggcttcgcg ccacattacg ggacaggcca tcgcagtcga
     3661 cggcggcgcc accatcatat gaatgaaaag ctcggaatga gtgaccggct cgcctttacc
     3721 cgcgactatc aaaggaagat cccgttcgtc tcgcacctga agatcctgac cgagacgctc
     3781 ggcgagggca ccgcgcggct ctcgctgccg atcgagctgc acctcaccaa cagcctcggc
     3841 accgctcacg gcggcgtgat cgtatcgctg ctcgacgtcg cgctgtgcac cgcggcgcgc
     3901 acgctgcacc cggagtcgat gggcgtggtc accatcgacc tcagcacctc tttcatcggc
     3961 ggcgccagcg gcagcaagct catcgccgag gcgcgcgtgc tgcgcgacgg ccgcagcatg
     4021 agcttcgtcg aggccgaggc gaagaacgag gacggctcgc tggtggcgaa agcgattgcc
     4081 accgtgcgcg tgcggcacaa gaaggacgca ggatgagaag cccgacgcgc ggcgtgcacc
     4141 acctcgcgct caccaccgag gacatgaagc tgacgaccga gttctacgtc ggcgtggtcg
     4201 gcatgccgct ggtgcacgcg atgaaggtgc cgccgggcct gggcagcggg ccgggcaacc
     4261 gcggcaaccc gccctacgag gagatccgcc attacttct
//
LOCUS       DASPXW010000140         5074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1806407, whole genome shotgun sequence.
ACCESSION   DASPXW010000140 DASPXW010000000
VERSION     DASPXW010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5074
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1806407"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="VEL04_03325"
     CDS             complement(<1..867)
                     /locus_tag="VEL04_03325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYT47427.1"
                     /translation="MKVAIVRQRYNPYGGAERFVARALTALERSGAEVTLVCRSAEGW
                     GARRVLRVDPFYLGNLWRDWSFARAARGAWRRQGFDVVQSHERIPGCDLYRAGDGVHR
                     RWLELRRAAAGPLERLGMVLNPYHRYVCSAERRMFEHPRLRAVICNSRMVRDEIQRAF
                     RVAPEKLHVIYSGVDLEHFHPRLGESLRGKTRAELGCRPRDTLFLFVGSGFARKGLDA
                     AIQALGQAGNEAFWLLVVGTDRDEARYKSLSKGLNVRFLGGREDVRPLYAAADCLILP
                     TRYDPFPNTVLEA"
     gene            complement(864..1922)
                     /gene="rfaQ"
                     /locus_tag="VEL04_03330"
     CDS             complement(864..1922)
                     /gene="rfaQ"
                     /locus_tag="VEL04_03330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipopolysaccharide heptosyltransferase
                     III"
                     /protein_id="HYT47428.1"
                     /translation="MLKDAVPLGECARALVVKLRHHGDVLLAAPVLTVLKAHAPRIEV
                     DALVYDDTAQMLEGHPALSKLHVTGRTWRQLGALQRFSRERELFKALRARRYDLLVHL
                     SEHPRGAWLARTLGARYRVGPIMADRGAFWRRSFSHLYPLIRNRHHVEVNLDALRRIG
                     IQPSMAERKVVFVPGAAAEKRIAQLVNGETFVHMHPASRWRFKCWPAERNAALIDRLA
                     ADGQNVVVTSAADEDEISLVKEILGKCQSTPVSLAGKLSIKELGALTARASLFIGVDS
                     MPMHLAAAMGTPTLALFGPSSEELWGPWNVERRVVTTTHSCRPCGVDGCGGGKVSECL
                     TLLGVDPVHAAARELLAR"
     gene            complement(1937..3913)
                     /locus_tag="VEL04_03335"
     CDS             complement(1937..3913)
                     /locus_tag="VEL04_03335"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYT47429.1"
                     /translation="MRHAISRSLVMWLAGCLISNCFAQEDAVIITATRFQDSKRDLPV
                     GITLITADDIRNSASSNLGDILAQYGLLQIRDLAGTQNPQLDLRGFGITGDQNTLVLL
                     DGLRLSENELVSAQLSSIPLESIERIEIVRGGGAVFYGGGASGGTINIITRRGEPGET
                     RARALERYGSFRTKEARAGLERQGETLGGAFAVTAEDTDGYRRNSRYRQTSFAGRLDA
                     VSGSDRAYLKLGADEQRQRLPGSLTEAEIQADPRQTKTPDDWSEREGAHVALGGVEQL
                     GAHQVSADLGFRQKHAKAFFAAFGGFFTDVEASVWSFAPRGKFVFDALGRKHDLILGA
                     DFDSWDYESRSASSPATVGAPFSHRVGEQQNSALYGQTNLWIAPTTRVVLGGRSQRVE
                     SHLAEQVFPTDDRRAVDHLTAYEAAVRHAFGAGWSAYTKYGKSFRVATFDENACFFPP
                     CAPALLKPQTALGGELGVEVERSGVRARVSAYQIDLEDEIYFSPLTFSNINLQPTRRR
                     GMELETQWRAAPTLDLRAALALLDARFRSGVYGGVDVSGKKVPLVPETLATAGLSWSY
                     ATRSRFNFNLRYVGRQRFDNDQANTFPRQQPDYTLADLKLEHGFERWDLAFEVRNLFD
                     RKYFSYGTATGPTSFSALPAPGRAAYASLAYRLD"
     regulatory      complement(3949..4122)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4217..4408
                     /locus_tag="VEL04_03340"
     CDS             4217..4408
                     /locus_tag="VEL04_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47430.1"
                     /translation="MDAEFNSLEAKVAQFVSLCERLRAENLTLRKELLAAQNDAKRLN
                     EKIDGAKSRLEGLLNRLPG"
     gene            4419..4730
                     /locus_tag="VEL04_03345"
     CDS             4419..4730
                     /locus_tag="VEL04_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HYT47431.1"
                     /translation="MAEGAKTIEVDLLGRTYRVACTDGEREALMQAVAYLDRKMNEIR
                     KAGKVMGAERIAVMAALNVAHELLSVRLGSGFDLGQAKRRISALESQLDAAIAKQEKL
                     F"
     gene            4740..4926
                     /gene="ssrS"
                     /locus_tag="VEL04_03350"
     ncRNA           4740..4926
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VEL04_03350"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            4937..>5074
                     /locus_tag="VEL04_03355"
     CDS             4937..>5074
                     /locus_tag="VEL04_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HYT47432.1"
                     /translation="MRYWLMKSEPDECSIDDALAAPGRRTPWSGVRNYQARNFMRDAM
                     RV"
BASE COUNT          851 a         1682 c         1732 g          809 t
ORIGIN      
        1 ggcttccagc acggtattgg gaaaggggtc ataccgggtc gggaggataa ggcagtccgc
       61 cgccgcgtac aggggccgca cgtcttccct gccgccgaga aagcggacgt tgaggccttt
      121 ggagaggctc ttgtaacgcg cctcatcccg atccgtaccg acgaccagca gccagaacgc
      181 ctcgttgccc gcctgcccga gcgcttggat cgccgcgtcg agccccttgc gcgcgaagcc
      241 cgagccgacg aagaggaaga gcgtgtcgcg cggccggcag ccgagctcgg cgcgcgtctt
      301 gccacgcagc gattcgccga gccgcggatg gaaatgctcg aggtcgacgc cgctgtagat
      361 cacgtgcagc ttctccggcg cgacgcggaa agcgcgctgg atctcgtcgc gcaccatgcg
      421 cgagttgcag atcacggcgc gcaggcgcgg gtgctcgaac atgcgccgct cggcgctgca
      481 gacgtagcgg tggtagggat tgaggaccat gccgagccgc tcgagcggtc ctgccgccgc
      541 ccgcctcagc tcgagccagc gccggtgcac gccgtctccg gcacggtaca ggtcgcatcc
      601 cggaatgcgc tcgtgcgact gcaccacgtc gaacccctgg cggcgccagg cgccgcgcgc
      661 ggcgcgggcg aacgaccagt cgcgccacag gttgccgaga tagaacggat cgacgcgcag
      721 cacgcgccgc gcgccccagc cctcggcgct gcggcaaacc agcgtcactt ccgcgccgga
      781 gcgctcgagc gcggtgagcg cgcgcgcgac gaagcgctcg gcgccgccat aggggttgta
      841 gcgctggcgc acgatcgcga ctttcacctc gccagcagct cccgcgccgc cgcgtgcacc
      901 gggtccacgc cgagaagcgt caggcattcg ctcaccttgc cgccgccgca gccgtcgacg
      961 ccgcagggac ggcaggagtg cgtcgtggtg acgacgcgcc gctcgacgtt ccacgggccc
     1021 cacagctcct cgctgctcgg cccgaagagg gccagcgtgg gcgtgcccat ggcggcggcg
     1081 agatgcatcg gcatcgagtc gacgccgatg aaaagactcg cccgcgcggt gagcgcgccg
     1141 agctccttga tcgagagctt gccggcgagg ctgacgggcg tgctctggca tttgccgaga
     1201 atttccttca ccagtgaaat ttcgtcttca tctgccgccg atgtcaccac cacgttctgg
     1261 ccgtcggcgg ccagccggtc gatgagcgcc gcgttgcgct ccgccggcca gcacttgaag
     1321 cgccagcgcg aggcgggatg catatgcaca aaggtctcgc cgttcaccag ctgcgcgatt
     1381 cttttctccg ccgccgcgcc cggcacgaac accaccttgc gctccgccat ggaaggctgg
     1441 atgccgatgc ggcgcagggc gtcgagattg acctcgacat ggtggcggtt gcggatcagc
     1501 ggatacagat ggctgaagct gcgcctccag aaggcgccgc ggtcggccat gatcggcccg
     1561 acgcggtagc gcgcgccgag cgtgcgcgca agccaggcgc cgcgcggatg ctcggaaaga
     1621 tgcaccagca ggtcgtagcg cctcgcgcgc agcgccttga acagctcgcg ttccctggag
     1681 aagcgttgca gggcgccgag ctgccgccat gtcctgcctg tcacgtgcag cttcgacaac
     1741 gccggatgac cttccagcat ctgagcagtg tcgtcgtaca cgagcgcatc gacctcgatg
     1801 cgcggcgcat gcgccttcag caccgtgagc accggcgcgg cgagcagcac gtcgccgtgg
     1861 tgcctcagtt tcaccaccag cgcgcgcgcg cactctccta gcgggacggc atccttcagc
     1921 accccacgag gttacctcaa tccagccggt aggccagcga agcgtaggcg gcgcgccctg
     1981 gggcgggcaa tgcgctgaag ctcgtcgggc cggtcgcggt gccgtagctg aagtatttcc
     2041 ggtcgaacag gttgcgcacc tcgaacgcca ggtcccagcg ctcgaagccg tgctcaagct
     2101 tcagatccgc gagcgtgtag tcgggctgct ggcgcggaaa agtgttcgcc tggtcgttgt
     2161 cgaagcgctg cctgccgacg tagcgcaggt tgaaattgaa gcggctgcgg gtggcgtagg
     2221 accacgacaa gcccgccgtc gccagcgttt ccggcaccag cggaaccttc tttccgctga
     2281 cgtcgacccc gccgtacacg cccgagcgga agcgcgcatc gagaagcgcg agcgcagcgc
     2341 gcaggtcgag cgtgggcgcc gcgcgccatt gcgtctcgag ctccatgccg cggcggcgcg
     2401 tcggctggag gttgatgttc gagaaagtga gcgggctgaa gtagatctca tcctcgaggt
     2461 cgatctggta ggcgctcacg cgcgcacgca ctccgctgcg ctcgacttcg acgccaagct
     2521 cgccaccgag cgcggtttgc ggcttcaaga gtgcgggtgc gcacggcggg aagaagcacg
     2581 cgttctcgtc gaaggtggcg acgcggaagc tcttgccgta cttggtgtac gccgaccagc
     2641 cagcgccgaa cgcgtggcgc actgctgctt cgtacgccgt gagatggtcc accgcgcggc
     2701 ggtcgtcggt cgggaatacc tgctcggcaa gatgggactc gacccgctgc gaacggccgc
     2761 cgagcaccac cctggtggtc ggcgcgatcc acaggttggt ctgtccgtag agcgcgctgt
     2821 tctgctgctc gccgacgcgg tgcgaaaacg gcgcgccgac cgtggccggg ctggaagcgc
     2881 tgcgcgactc gtaatcccag ctgtcgaagt ccgcgccgag aatcaggtcg tgcttgcgcc
     2941 cgagcgcatc gaagacgaat ttgccgcgcg gcgcaaagct ccagacgctc gcctcgacgt
     3001 cggtgaagaa gccgccgaac gcagcgaaga acgccttggc gtgcttctgg cggaagccga
     3061 ggtcggccga gacctggtgc gcgccgagct gttccacgcc gccaagggcg acgtgcgcgc
     3121 cttcgcgctc gctccaatcg tcgggggttt tcgtctggcg cggatcggct tgaatctcgg
     3181 cctcggtgag cgagcccggc aggcgctggc gctgctcgtc ggcgcctagc ttgagatagg
     3241 cgcggtcgga gccggagact gcgtcgagac gcccggcgaa gctggtctgg cgatagcggc
     3301 tattgcgccg gtagccgtcg gtgtcttccg ccgtgaccgc gaaggcgccg ccgagcgtct
     3361 cgccctggcg ctcgagcccg gcccgcgctt ccttggtgcg aaagctgccg tagcgctcga
     3421 gcgcccgcgc gcgcgtctcg cccggctcgc cccggcgcgt gatgatgttg atggtgccgc
     3481 cgctggcgcc gccgccgtag aacaccgcac cgccgccgcg cacgatctcg atgcgctcga
     3541 tcgattcgag cggaatcgag ctgagctgcg ccgagacgag ctcgttttcg ctcagccgca
     3601 ggccgtcgag caggaccagg gtgttctggt cgccggtgat gccgaagccg cgcaggtcga
     3661 gctgcgggtt ctgcgtgcca gcgagatcgc ggatctgcag caggccgtac tgcgcgagga
     3721 tgtcgccgag gttggacgag gcgctgttgc ggatatcgtc ggccgtgatc agcgtgatgc
     3781 cgacgggcag gtcgcgcttc gaatcctgga agcgcgtcgc ggtgatgatg acggcgtcct
     3841 cctgagcaaa acaattggaa ataaggcagc cggcgagcca catgacaagc gagcggctta
     3901 tggcgtgacg catggaaacc tccctggcgt cttccccgac gccttaagtg gttgcgaagg
     3961 cttccaggcc ggtctccgga ctcgcgaggc atagcattcc tgccttcccg cgcccgactg
     4021 agcgcagtgg ccaaatctgc ggaatgcttc tctcgcctac cgttgcgggg gcagtagagg
     4081 cttgggacct ctttcccgta cacctggata gcgaaccgcc atcataaagg ggattcgcca
     4141 gccctgatga tggaatacat gcaaaaacac cgcttactgc ttgacgatgc ttgaattttg
     4201 aaacatagtt cgacgcgtgg acgcggagtt caattcgctc gaagccaagg tcgcgcagtt
     4261 cgtatcgctg tgcgagcggc tgcgcgccga gaacctcacg ttgcgcaagg agctgcttgc
     4321 cgcgcagaac gacgccaagc gcctgaacga gaaaatcgac ggcgcgaaat cgcggctcga
     4381 aggcctcctc aaccggctgc ccggctgacg cgcgagtgat ggccgagggc gccaagacca
     4441 tcgaagtcga cctgctcggt cgcacttacc gggtggcgtg caccgacggc gagcgcgagg
     4501 cgctgatgca ggcggtggcc tatctcgacc gcaagatgaa cgagatccgc aaggccggca
     4561 aggtcatggg cgccgagcgc atcgcggtga tggcggcgct gaacgtcgcg cacgagctgc
     4621 tgagcgtcag gctcggctcg gggttcgatc tcggccaggc gaagcgccgc atctcggcgc
     4681 tcgaatcgca gctcgacgcg gcgattgcaa aacaagaaaa actattctaa aagatagcgt
     4741 gttccctgct gtgcacgacc gggctcaata tttccgaacc gatagcttcc ccaggttgcg
     4801 ggccctagtg tcactgttgt gatcgtccca cgcttgcgtg ccggacgaac ctgatgtgac
     4861 cggtaagcga ccaccctgtt tagccagggt tcgggagccg agcccaccgg cataagcggg
     4921 gaacaccttt ccaccgatgc gatactggct catgaagagc gagcccgacg agtgctcgat
     4981 cgacgacgcg ctcgccgcgc cgggccggcg cacgccctgg agcggcgtgc gcaactacca
     5041 ggcgcgcaac ttcatgcgcg acgcgatgcg cgtc
//
LOCUS       DASPXW010000141         3658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_547816, whole genome shotgun sequence.
ACCESSION   DASPXW010000141 DASPXW010000000
VERSION     DASPXW010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3658
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547816"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..909
                     /locus_tag="VEL04_03360"
     CDS             160..909
                     /locus_tag="VEL04_03360"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT47433.1"
                     /translation="MTLLRTEALKKHFGETRAVDGVDFTVNEGEFLSLVGTNGAGKTT
                     LVNLISAFLLPDSGRIFIGDRDITHASVEQRIRAGIARSFQLVNLFDDLSVLDNVALA
                     IFAREGKTKRIFAIAARDAAVSREASDLLGEFGLGGKSEARAGGLAQGERKLLDVAVA
                     YALRPKLLFLDEPTSGVSTRDKAQIMDTVSAVVRGGGITAVVIEHDMDVVFKYSDRVV
                     VMHEGRFLADGPPEEIRQNSDVAKYLLGTDV"
     gene            911..1630
                     /locus_tag="VEL04_03365"
     CDS             911..1630
                     /locus_tag="VEL04_03365"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT47434.1"
                     /translation="MLEVKNIDAWRGPAHILHQVSLSVREHEVVCLVGRNGAGKTTTM
                     ESIMGFLPVRSGQILFHGEEVTSLPVHQRARRGMGYAPEGSEIFPELTVAENMMISRW
                     MSAKARRRLEALADGDVEERVFAVFPEVRELLRRQGMNLSGGQRKMVAIARGMALAPT
                     LLLLDEAFEGLAPVVVKRFRDAVLKIEDLGISLLIAESNLVNAARIADRLYAIDRGEI
                     IFEGKPEQALGDANVMRALRG"
     gene            complement(1608..1985)
                     /locus_tag="VEL04_03370"
     CDS             complement(1608..1985)
                     /locus_tag="VEL04_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015603733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HYT47435.1"
                     /translation="MMADALLVVHFMVVVFIVGGLVLVWAGAALGWPWVRNPWFRYLH
                     LGAIAFVAAEALLGVACPLTVWEDLLRGGVRPESFIGRWAYWLLYYQAPEWVFTTAYA
                     AWALATLVTLRLVPPTLSRATPS"
     gene            2037..2552
                     /locus_tag="VEL04_03375"
     CDS             2037..2552
                     /locus_tag="VEL04_03375"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HYT47436.1"
                     /translation="MTPEQRAVTAEIAAGPRGEVRGPFLALLHHPELARRLQALGEQL
                     RWKAKLAPHLVELAVLVTARRWTCQHEWHMHEKLARKHGLEERIVTAIAKGREPEGMP
                     ADEAAVHAFCRELHASGRAGDATFDAVRRRFGLDGALELIALCGYYSLMAMVLNTAGM
                     PLPDDAPPPLK"
     gene            2549..3376
                     /locus_tag="VEL04_03380"
     CDS             2549..3376
                     /locus_tag="VEL04_03380"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYT47437.1"
                     /translation="MKHACDAHFHVFGPPERYPYRDPKLRYQPPLAPLADYLETARKL
                     GLERFVFVQPSAYGRDNACMLDAMREVGARCRGIVDIDEDAPDAELARLHAAGVRGVR
                     INVSPMQPHDAGIAAKLLPRIERLEARCAALGWQLDFLLPGWLTAELMPRLSNLKVTF
                     TLAHMGMFLAREGPRHPGFRKLLELLRRGSGRCWVKLTGAYRMSQAPGFADAAPMARA
                     LIEAAPDRMIWGSDYPHLSFADKVDSVELSDLLREWARDEAALRKILVDNPARLYGF"
     gene            complement(3400..>3658)
                     /locus_tag="VEL04_03385"
     CDS             complement(3400..>3658)
                     /locus_tag="VEL04_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HYT47438.1"
                     /translation="GALNRFNADNISADGTFKPASELKREFQALLGKTKNKEIVHYCG
                     SGVAACHNLLAMEIAGLPGARLYAGSWSEWSADPKRPQERG"
BASE COUNT          610 a         1229 c         1236 g          583 t
ORIGIN      
        1 cccgatcgtc ggggcggtga tcttcaacta cctcgaggtc tacgccatcg cgctcaccga
       61 atactggcag atcacgcttg gcgcgatcct gatcgtgcta gtgaccttcc tcccggcagg
      121 aatcgtcggc actgcgcaac gcttgtcggc gaagttcaaa tgactctttt acggacagag
      181 gccctcaaga agcacttcgg cgaaacgcgc gccgtggacg gggtggattt caccgtcaac
      241 gaaggcgagt tcctctcgct cgtcggcacc aacggcgcgg gcaagaccac gctggtcaac
      301 ctgatcagcg cgttcctgct ccctgacagc ggcaggatct tcatcgggga ccgcgacatc
      361 acccatgcgt cggtggaaca gcgcatccgg gcggggatcg cgcgcagctt ccagctcgtg
      421 aacctgttcg acgatttgtc ggtgctcgac aacgtggcgc tcgcgatctt cgcgcgcgaa
      481 ggcaagacca agcggatttt tgcaatcgcc gcgcgagacg ccgcggtgag ccgcgaagcg
      541 tcggatctgc tcggcgagtt cggcctcggc ggcaagtcgg aggcgcgcgc gggagggctc
      601 gcgcagggcg agcgcaagct cctcgacgtg gcggtcgcct acgcgctgcg ccccaagctg
      661 ctgttcctcg acgagccgac cagcggcgtg agcacgcgcg acaaggcgca gatcatggac
      721 accgtgtctg cggtggtgcg cggcggcggc atcaccgcgg tggtcatcga gcacgacatg
      781 gacgtggtgt tcaagtactc cgatcgcgtc gtcgtgatgc acgaaggcag gttcctcgcc
      841 gacggcccgc ccgaggagat ccgccaaaac agcgacgtcg ccaagtacct gctcgggacc
      901 gacgtttgac atgctggaag tgaaaaacat cgacgcctgg cgtggcccgg cgcacatcct
      961 gcaccaggtt tcgttgagcg ttcgcgagca cgaggtcgtt tgcctggtcg gccgcaacgg
     1021 cgcgggcaag accacgacca tggagagcat catgggcttt ctgccggtca ggtccggcca
     1081 gatcctgttt cacggcgagg aggtgacttc gttgcccgtg caccagcggg cgcggcgcgg
     1141 catgggctac gcgcccgaag ggtccgagat cttccccgag cttaccgtgg ccgagaacat
     1201 gatgatcagc cgctggatgt cggcgaaggc gcgccgcagg ctggaggcgc tcgccgacgg
     1261 ggacgtcgag gagcgggtat tcgcggtgtt ccccgaagtg cgcgagctgc tgcgccgcca
     1321 ggggatgaac ctgagcggcg gccagcgcaa gatggtcgcc atcgcgcgcg gcatggcgct
     1381 tgcgcccacg ctgctgctcc tggacgaggc gttcgagggg ctcgccccgg tcgtggtcaa
     1441 gcggtttcgc gacgccgtgc tcaagatcga ggacctcggc atctccctcc tcatcgccga
     1501 gtcgaacctc gtcaatgcgg cgcgcatcgc ggaccggctc tacgcgatcg accgcggcga
     1561 gatcattttc gagggcaagc ccgagcaggc gctcggcgac gcaaacgtca tgagggcgtt
     1621 gcgcggctga gcgtcggcgg caccagccgg agagtgacga gcgtggcgag agcccaggct
     1681 gcatacgcgg tggtgaacac ccattccggg gcctggtaat agaggagcca gtacgcccag
     1741 cggccgatga aggactccgg ccgcactccg cccctcagca ggtcttccca taccgtgagc
     1801 gggcaggcga cgccgagcag cgcttccgcg gcgacgaaag cgatggcgcc aagatgcagg
     1861 tagcggaacc aggggtttct cacccacggc cagccgagcg ccgcgccggc ccagaccagg
     1921 accaggccgc cgacgatgaa gacgacgacc atgaagtgga ctaccagcag cgcgtcggcc
     1981 atcatgcgcc gaggatgaca cgagaaccgc ggttcccccg cctgacgata gacctgatga
     2041 cgcccgagca gcgcgcagtc accgccgaga tcgccgccgg cccgcgcggc gaggtgcgcg
     2101 ggcccttcct cgccctgctc caccacccgg agctcgcgcg ccgcctgcag gcgctcggcg
     2161 agcagctgcg ctggaaggcg aagctcgcgc cgcatctcgt cgagctcgcg gtgctggtca
     2221 ccgcgcggcg ctggacctgc cagcacgagt ggcacatgca cgagaagctg gcgcgcaagc
     2281 acgggctgga agaacgcatc gtcacggcca tcgccaaagg ccgcgagccc gaaggcatgc
     2341 cggccgatga agcagcggtg cacgcattct gccgcgagct gcacgccagc ggccgcgccg
     2401 gcgatgcaac cttcgacgcg gtgcgcaggc gcttcgggct cgacggcgcg ctcgagctga
     2461 tcgcgctgtg cggctattac tcgctcatgg cgatggtgct gaacaccgcc gggatgcctc
     2521 ttccagacga tgctccgccg ccgctgaagt gaagcacgcc tgcgacgcgc atttccacgt
     2581 cttcggcccg cccgagcgct acccctatcg cgacccgaag ctgcgctatc aaccgccgct
     2641 cgcgccgctc gccgactacc tggagacggc aagaaaactc ggcctggagc gcttcgtgtt
     2701 cgtgcagccg agcgcttacg gccgcgacaa cgcctgcatg ctcgacgcca tgcgcgaggt
     2761 cggcgcgcgc tgccggggca tcgtcgacat cgacgaagac gcgcccgacg cggagctcgc
     2821 gcggctgcac gctgccggcg tgcgcggcgt gcgcatcaac gtctcgccga tgcagccgca
     2881 tgacgccggg attgcggcga agctcctgcc gcgcatcgag cggctcgagg cgcgctgcgc
     2941 cgcgctcggc tggcagctcg attttctgct tcccgggtgg ttgacggcgg agctgatgcc
     3001 tcgtctttca aatttgaagg tgaccttcac gctggcgcac atgggcatgt tcctcgcgcg
     3061 cgaggggccg cgccatcccg gcttcaggaa gctgctggag ctcctgcggc gggggagcgg
     3121 ccgctgctgg gtgaagctca ccggcgccta ccgcatgtcg caggcgcccg gctttgccga
     3181 cgctgcgccg atggcgcgcg cgctcatcga agccgcgccc gaccggatga tctggggcag
     3241 cgactacccg cacctctcat tcgccgacaa ggtcgattcg gtcgagctgt ccgatctgct
     3301 gcgcgagtgg gctcgcgacg aagccgcgct gcgcaagatc ctggtggata acccggcgcg
     3361 gctatacggc ttctagccgc gttcctgcgg tctctgcatt cagccgcgtt cttgcggcct
     3421 ctttggatcg gcgctccact cgctccacga tcccgcatag agcctcgcac cgggaagccc
     3481 ggcgatctcc atcgcgagca ggttgtggca ggcggcgacg ccggagccac agtagtggac
     3541 gatttccttg ttcttcgttt ttccaagcaa tgcttgaaat tcgcgcttca actctgaggc
     3601 aggcttgaaa gtgccgtccg ccgaaatatt gtcggcgttg aagcgattga gcgcgccg
//
LOCUS       DASPXW010000142         4178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1430505, whole genome shotgun sequence.
ACCESSION   DASPXW010000142 DASPXW010000000
VERSION     DASPXW010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1430505"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1222)
                     /locus_tag="VEL04_03390"
     CDS             complement(<1..1222)
                     /locus_tag="VEL04_03390"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HYT47439.1"
                     /translation="MILPATRLLWLLLALAAAAVAVSLYPDFLPYWTTLGGVFLLLAA
                     LDAVAGIRLPAPAVVRQVPGSLALGVPVEVRLRVRNLSGLRLRCEVHDHHPASVECEG
                     LPRPLALAPQEWGELSYLVRPVARGEARFLPAEIRVSSPLKLWQVTRRAGGETSIRVY
                     PNFRALARYTLLATDNRLSQIGVLQVRRRGEGTEFHQLREYRQGDSQRAIDWKATART
                     ARLIAREYEDEKDQRVLLVVDCGRRMAAKDDSADDVLSHFDHTLNAALLLAHVAARQG
                     DAVGMLTMGGVSRYCEPRKSLAAVHVMMNRAYDIEPTLAVPDYQLAATDVMRHLRRRA
                     LVILLTNLRDEDDDGLMPALQLLRTRHLVVLASLREAIIARALTARVDSFDRAVTHAA
                     AADYLVARERVFRRI"
     gene            complement(1219..2205)
                     /locus_tag="VEL04_03395"
     CDS             complement(1219..2205)
                     /locus_tag="VEL04_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HYT47440.1"
                     /translation="MSAQPAPSAEQFQQAAKVAVAMRAEIGRALVGQKEVVDAVLCAL
                     LAGGHVLVEGVPGLGKTLLVQALARTISGSFGRIQFTPDLMPADVTGHTLYDPKNQVF
                     TARRGPVFVNLLLADEINRAPAKTQAALLEVMQEGQVTIEGRAHALEPPFMVLATQNP
                     IEQEGTYALPEAQLDRFLLKVQIDYPTQADEAAMVRQVTDGKVGDRLNVEEVASLVKP
                     ATVVTLQAIAAQLRTDDAVLDYAIRLVRATRSWAGVAAGAGPRGSISLLRAARGAALL
                     EGRDYVTPDDVKRMALPALRHRIRPSPELELEGRDADTILKGLLEKIEAPRK"
     gene            complement(2202..3320)
                     /locus_tag="VEL04_03400"
     CDS             complement(2202..3320)
                     /locus_tag="VEL04_03400"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HYT47441.1"
                     /translation="MRARLLVPIGIAAALAAGVLWFYANFERVPAKVWVGPSGEARLR
                     QFLAAERFAERMGLRTKELRSLPEIDALAPGGVLLLPNRRQALDARRVERILAWVQGG
                     GHLIAEAESQGVPDPLLDLLAVRRTAAAALSSPLAIESAGGARSFKVALHGRLALEPP
                     TGRLQLRAAGGEGIRLASFTRGKGLVTVATSLDFARNGLIGEADHAEFLWFLMQSTPA
                     AELKVYMRPVRLSLWGFLMEHAVAALAAGALLLAAWLWRIAPRFGPVAPDAPPARRRL
                     LDHLRASGRYYWAQGLRERLVTAARDAALRRVVRVQPEFVAARGDERAERVASLVAIS
                     QQEAERLVSASGAMRGAAFIQLVQSAQRVHAALERGNR"
     gene            complement(3317..>4178)
                     /locus_tag="VEL04_03405"
     CDS             complement(3317..>4178)
                     /locus_tag="VEL04_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47442.1"
                     /translation="SSGDALSYGDLLAYAAAVCVLEPFYVAAGFALYLNRRTALEGWD
                     IEVALRQIAQRHTAAVATMILMFLACLWMQMPSQAADRNPRQEIAEVLKAPEFPHYRE
                     TQRWEPRDPRPAKEPRNSSDGNWLRGIGFALAKAAQVLFWALAGCALAYALWWAARML
                     PRGRAPAAGPYRAPASLFGMELAPEKLPSDVAAAAAALARAGRVREALGLLYRGALSD
                     LVHRRGVELLASHTEAEALAVACTKLNVPGGLYLETLVKAWRESAYARRDPAPRELER
                     LAQDYRSFAA"
BASE COUNT          609 a         1497 c         1513 g          559 t
ORIGIN      
        1 cgatgcgccg gaacacgcgc tcgcgggcca cgaggtaatc ggcagcggcg gcatgcgtca
       61 cggcgcgatc gaagctgtcg acccgggcag tgagcgcgcg cgcgatgatc gcttcgcgca
      121 gcgacgccag caccaccagg tgccgcgtgc gcagcagctg gagcgccggc atcagcccgt
      181 cgtcgtcctc gtcgcgcagg ttggtgagca ggatgacgag cgcgcgcctt cgcagatggc
      241 gcatgacgtc ggttgccgcc agctggtagt cgggcaccgc gagggtgggc tcgatgtcgt
      301 aggcgcggtt catcatcacg tgcaccgcgg cgagcgactt gcgcggctcg cagtagcggc
      361 tgacgccgcc catggtgagc atgcccaccg cgtcgccctg gcgggccgcg acgtgcgcca
      421 gcagcagcgc ggcgttcagc gtgtggtcga agtgcgacag aacgtcgtcc gcggagtcat
      481 cctttgctgc catgcgccgg ccgcagtcca ccaccagcag cacgcgctgg tccttctcgt
      541 cctcgtactc gcgcgcgatc aggcgcgcgg tgcgcgcggt cgccttccag tcgatcgcgc
      601 gctgcgaatc gccctgccgg tactcgcgca gctggtggaa ctcggtgcct tcgccgcgcc
      661 ggcgcacctg cagcacgccg atctgcgaca ggcggttgtc ggtcgctagc agcgtgtagc
      721 gggcgagcgc gcggaagttg ggatagacgc ggatcgaggt ttctcctcct gctcgccggg
      781 tgacctgcca aagctttaat ggtgaggaga cgcgaatctc ggccggcaga aagcgcgctt
      841 cgccgcgcgc cacggggcgc accaggtagc tcagctcgcc ccactcctgc ggcgccagcg
      901 cgagcggccg cggcagcccc tcgcactcga cgctcgccgg atgatggtcg tgcacttcgc
      961 agcgcagccg cagccccgag aggttgcgca cgcgcagccg cacctcgacc gggaccccga
     1021 gcgcgagcga gcccggcacc tggcggacca ccgccggggc gggcagccgg atgcccgcca
     1081 cggcatccaa cgcggcgagc agcaggaaca cgccgccgag cgtggtccag taaggcagga
     1141 aatcggggta gagcgacacc gcgacggcag ccgcggcgag cgcgaggagc agccacagga
     1201 gacgtgtcgc cggcagaatc acttgcgtgg tgcttcgatc ttttccagca ggcccttgag
     1261 gatcgtgtcg gcgtcgcggc cctcgagctc gagctcgggc gagggccgga tgcggtggcg
     1321 cagcgcgggc agggccatgc gcttcacgtc gtcgggggtg acgtagtcgc ggccttccag
     1381 gagggcggcg ccacgcgccg cccgcagcaa cgagatgctg ccgcgcgggc cggcgcccgc
     1441 ggcgacgccg gcccagctgc gcgtggcgcg caccaggcgg atcgcgtaat cgagcaccgc
     1501 gtcgtcggtg cgcagctgcg ccgcgatcgc ctgcagcgtc accaccgtcg caggcttgac
     1561 caggctcgcc acctcctcga cgttcagccg gtcgccgacc ttgccgtcgg tgacctggcg
     1621 caccatcgcg gcctcgtcgg cctgcgtcgg gtagtcgatc tgcaccttga gcagaaagcg
     1681 gtcgagctgc gcctccggca gcgcgtaggt gccctcctgc tcgatcgggt tttgcgtcgc
     1741 gagcaccatg aacggcggct cgagggcgtg ggcgcggccc tcgatcgtca cctggccttc
     1801 ctgcatcact tccagcagcg cggcctgggt tttggccggc gcgcggttaa tctcgtcggc
     1861 cagcagcaga tttacaaaga ccggtccgcg tcgtgctgtg aatacctggt tcttggggtc
     1921 gtagagcgta tgcccggtga cgtcggcagg catcaggtcg ggcgtgaact ggatgcgccc
     1981 gaagcttccc gagatggtcc tggcaagcgc ctggacgagc agcgtcttac cgaggccggg
     2041 aacgccttcg accagcacgt ggccgccggc gagcagcgcg cacagcaccg cgtcgacgac
     2101 ctccttctgg ccgacgagcg ccctgccgat ctcggcgcgc atcgcgacgg cgactttcgc
     2161 cgcctgctgg aattgctccg cgctgggcgc gggctgggca ctcatcggtt ccccctttcg
     2221 agcgcggcat gcacgcgctg cgcggactgc acgagctgga tgaacgcggc gccgcgcatc
     2281 gcgcccgacg cggataccag gcgctccgcc tcttgctgcg aaatcgcgac cagtgaggca
     2341 acgcgctcgg cacgttcgtc gccgcgcgcg gcgacgaact ccggctgcac gcgcaccacg
     2401 cgccgcagag cggcgtcgcg cgccgccgtc accaggcgct cgcgcaagcc ctgcgcccag
     2461 tagtagcggc cgctcgcacg caggtgatcg aggagccggc gccgggcggg cggcgcgtcc
     2521 ggcgcgaccg ggccgaagcg cggcgcgatg cgccacagcc aggcggcgag cagcagcgcg
     2581 cccgcggcga gcgccgccac cgcgtgctcc atcaggaaac cccacagcga caggcgcacc
     2641 gggcgcatgt acaccttcag ctcggcggcg ggcgtcgact gcatcaggaa ccagaggaac
     2701 tcggcgtggt cggcctcgcc gatcaagccg ttgcgcgcga agtcgaggct cgtcgccacg
     2761 gtcaccaggc ccttgccgcg cgtgaacgag gcgagccgga tgccctcgcc gccggccgcg
     2821 cgcagctgca gccttccggt gggcggctcg agcgcgagcc ggccgtgcag cgcgaccttg
     2881 aagctgcgtg cgccgcccgc ggactcgatc gcgagcgggc tggagagcgc ggccgcggcc
     2941 gtgcgccgca ccgccagaag gtcgagcagc ggatcgggca cgccctggga ctcggcctcg
     3001 gcgatgaggt ggccgccgcc ctgcacccag gcgaggatgc gctcgacgcg gcgggcgtcg
     3061 agcgcctgcc tgcggttcgg cagcagcagc acgccgccgg gggcgagcgc gtcgatctcc
     3121 ggcagcgagc gcagctcttt cgtgcgcagt cccatgcgct cggcgaagcg ctcggcggcg
     3181 aggaactgcc tcaggcgcgc ttctcccgag ggaccgaccc ataccttggc cggcacgcgc
     3241 tcgaaattcg catagaacca cagcacgccg gcggcgagcg cagcggcgat gccgatggga
     3301 accagcaggc gcgccctcat gcggcgaagc tcctgtagtc ctgcgccagg cgctcgagct
     3361 cgcgcggcgc gggatcgcgc cgcgcgtagg ccgactcgcg ccacgccttg accagcgtct
     3421 cgaggtacag gccgccaggc acgttcagct tggtgcacgc gaccgctagc gcctcggcct
     3481 cggtatggct cgcgagcagc tcgacgccgc gccggtgcac cagatcggaa agcgcgcccc
     3541 ggtagagcag cccgagggcc tcgcgcaccc tgcccgcgcg cgcgagcgca gccgccgccg
     3601 cggcgacgtc gctcggcagc ttttccggcg ccagctccat gccgaagagc gaagcgggcg
     3661 cgcggtaggg cccggcggcc ggcgcgcggc cgcgcggcag catgcgcgcc gcccaccaca
     3721 gcgcgtaggc gagggcgcaa ccggcgagcg cccagaacag cacctgggcg gccttggcga
     3781 gcgcgaagcc gattccccgc agccagttgc cgtcgctgct gttgcgcggt tcctttgccg
     3841 gccgcgggtc gcgcggctcc cagcgctgcg tttcgcggta atgcggaaac tcgggcgcct
     3901 tgagcacttc ggcgatttcc tggcggggat tcctgtccgc ggcttgtgaa ggcatctgca
     3961 tccaaaggca ggcaaggaac atcaatatca ttgtggcaac ggcggcggtg tgccgctgcg
     4021 cgatctggcg cagcgccacc tcgatgtccc agccctcgag ggccgtgcgg cggttgaggt
     4081 agagcgcgaa cccggcggcg acgtagaacg gctcgagcac gcagaccgcc gcggcatagg
     4141 ccaggagatc gccgtaggac agcgcatcgc ccgacgac
//
LOCUS       DASPXW010000143         6329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_673702, whole genome shotgun sequence.
ACCESSION   DASPXW010000143 DASPXW010000000
VERSION     DASPXW010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6329
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673702"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VEL04_03410"
     CDS             <1..272
                     /locus_tag="VEL04_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYT47443.1"
                     /translation="ELPAIDESLPGYEFDSWIGLLGPAAMPKAMVESINAAVAKLLQD
                     PVILGRLDKQGVEPRAMTPAEFSSLLKSDFAKMAKVVKASGARID"
     gene            complement(280..465)
                     /locus_tag="VEL04_03415"
     CDS             complement(280..465)
                     /locus_tag="VEL04_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012354021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47444.1"
                     /translation="MKTLSILLIAAAALTGCAVVPYPEPAGVYVAPPVVVARPYYYGY
                     YGYRGYYGNPYWHRGWR"
     gene            complement(524..1225)
                     /locus_tag="VEL04_03420"
     CDS             complement(524..1225)
                     /locus_tag="VEL04_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HYT47445.1"
                     /translation="MKAEENRLHAYLDGELDAAASLEFEAQMAENPALRAACERQREL
                     SAAIRGKADYHPAPIRLYRVEQPASKSFKFISAFALVALVAFGLGTLLTFRAAEDPLA
                     REVVASHVRATLGGRLIDVASSDQHTVKPWLSARLPFSPPVTDLSQDGFELAGARLDY
                     AAGQPVAVLVYKRRQHVVDVFVWPAAGESEISASARDGFNVARFARGGMRYWVVSDLN
                     RNELDDFARLLAARR"
     gene            complement(1222..1746)
                     /locus_tag="VEL04_03425"
     CDS             complement(1222..1746)
                     /locus_tag="VEL04_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYT47446.1"
                     /translation="MTDRRARFEQQVLPHLDAAFNLARWLTRNDHDAEDVVQEALLRA
                     YRFFDGLRGAPRPWLLAIVRNACFSWLQANRPAELHAVDGMEQESDAPGPEALAARDV
                     DRRRLNEAIAALPVQFREVLVLRELEDLTYKDIARIAEIPIGTVMSRLSRARRQLAEA
                     LRIIAAPPRLRVHK"
     gene            complement(1771..2130)
                     /locus_tag="VEL04_03430"
     CDS             complement(1771..2130)
                     /locus_tag="VEL04_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HYT47447.1"
                     /translation="MTRRRLLSGALAAFLAGAGAWSLAAGPKEKVVKIAARKFTYAPN
                     IVTLKKGVPVVLEFTSGDVVMGFNAPDLNIRADIIPGQTARLRFTPDKAGSFVYLCDI
                     FCGDGHERMTGKIRVVA"
     gene            complement(2127..3008)
                     /locus_tag="VEL04_03435"
     CDS             complement(2127..3008)
                     /locus_tag="VEL04_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HYT47448.1"
                     /translation="MIDRRSFLRIAGVGGVVFASRLARGADAYATSQDEFFFVQLSDT
                     HWGFQGAANPDALVTLPKAIAAVNALDPQPDFIVFTGDLTHTTDDPAERRKRLAKFKE
                     LISSLKNKNIRLMPGEHDGSLDRGEAFKEFFGATHYTFDHKGVHFIALDNVSDPAARI
                     GDEQLAWLKSDLERQKADARIVVLTHRPLFPLFPAWDWATRDGDRAIELLMPHRNVTV
                     FYGHIHQEHHFKTAHIEHHAAKSLIFPLPVAGSQPKRVPLAWDPAQPYRGLGFRRVEA
                     EAQPVKYKISEMPVVKV"
     gene            3079..4164
                     /locus_tag="VEL04_03440"
     CDS             3079..4164
                     /locus_tag="VEL04_03440"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYT47449.1"
                     /translation="MRLGLFMMPMHPAHRDPAETLQEDRETVILADRLGFHDAFVGEH
                     LTDRCENVTNSFIFLATLIAETKTIKLGTGTSNLSHAHPTLIAAHAAMFDHLAKGRFI
                     FGISPGALASDAEALGMPLPMEERNKLFAEAIDVILAIWERDAPYDIDLPGNRFKVST
                     AKTLLPELGRGFMYKPYQKPRPEIVGTVVAPHSKGVIAMGERDFHPMSANFLLPHWLP
                     SHWKNYAEGKGRKGQQANPADWRVARTIFVADDDKTAKRYALEDPASPYRFYWEKLRG
                     NMIRARRHVIFKLHEQEDDSAVTLERLLDQLVICGSANRVADQILALHERAGDFGELV
                     YAGMDWVDPRLARRSMELMAEKVLPQL"
     gene            4299..4928
                     /locus_tag="VEL04_03445"
     CDS             4299..4928
                     /locus_tag="VEL04_03445"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006384906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYT47450.1"
                     /translation="MGLKPQELARIAELTLEHYERRADDFWRGTRDHDVSQNIAELLR
                     HIEGEPPFRILDLGCGPGRDLKAFTALGHIAVGLEGAPRFAAMAREYSGCEVWEQDFL
                     KLDLPPARFDGIFANAALFHVPGQELHRVLAELRAALKPRGVLFSSNPRGHNEEGWNR
                     GRYGAYHDLGTWRRYLSAAGFVELNHYYRPPGLPREQQPWLASVWRREK"
     gene            4996..>6329
                     /locus_tag="VEL04_03450"
     CDS             4996..>6329
                     /locus_tag="VEL04_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HYT47451.1"
                     /translation="MKKLTVAAFLAVVPLFAFAQKVGPSGIGGVVTGPKGPEAGVWVI
                     AETTDLPTKYAKVVVTDDKGRYLIPELPQANYTVWARGYGVKDSAKSKAARGEAVNIK
                     VAAASAKEDAEHYPGMYWYSMINIPAKNQFPGTGEKGNGISDNIKTQEQWVDTVKNAC
                     QSCHSLGSKGMRTVPKEFGPGVPGWARRTQSGQALTQMALGLGYMGADAALKNFADWT
                     DRIAAGELPFAKPERPKGVERNMVVTMWDFSTPKYYLHDGISTDRDNPRLNANGPIYG
                     APEESTDNIPVLDPVKHKAHTIKHPVINPNTPSSTSLPMQPSPYWGKEPIWDGHSSIH
                     NAMMDSEGRVWFTARIRPNANPDFCKKGSDHPSAKVAPLDASLRQVSVYDPKTEKWRH
                     VDTCFTTHHLYFAKDANRTLWLSQGGPASGVVGWVNTKLFLETGDSARSQGWT"
BASE COUNT         1104 a         2071 c         2142 g         1012 t
ORIGIN      
        1 ccgagctgcc ggcgatcgac gagagcctgc ccggctacga gttcgattcc tggatcgggc
       61 tgctcgggcc ggcggcgatg ccgaaagcga tggtcgagag catcaatgcc gcggtggcga
      121 agctgctgca ggacccggtg atcctcggcc gactcgacaa gcagggggtc gagccacgcg
      181 cgatgacccc cgctgagttt tcaagcttgt tgaagagcga ctttgccaag atggcgaagg
      241 tggtgaaggc gtcgggtgcg cgcatcgact gacgcgcgat cagcgccagc cgcggtgcca
      301 gtacgggttg ccgtagtacc cccggtagcc gtaatacccg tagtagtagg gacgcgccac
      361 gaccacgggc ggggccacat agacgccggc gggctccggg tacggcacga ccgcgcagcc
      421 ggtcagggcg gcggctgcga tcagcaggat ggagagtgtt ttcataagcc cattaaacgc
      481 gcttgggcgc cgtccatcct cgcaaatttg ttacaagatt ttgctagcga cgagccgcga
      541 gcagcctggc gaagtcgtcg agctcgttgc ggttgaggtc ggagaccacc cagtagcgca
      601 tgccgccgcg cgcgaagcgc gcgacgttga agccgtcgcg cgcgctggcg ctgatctccg
      661 actctccggc ggcgggccag acgaagacgt cgacgacgtg ctggcgccgc ttgtacacca
      721 gcaccgccac cggctggccg gcggcgtaat cgagccgcgc gccggcgagc tcgaagccgt
      781 cctgcgacag gtccgtcacc ggcggcgaga aaggcagccg cgcgctgagc cagggcttca
      841 ccgtgtgctg gtccgaggag gcgacgtcga tgaggcggcc gccgagcgtg gcgcgcacat
      901 ggctcgccac gacttcgcgc gccagcgggt cttccgctgc acggaacgtg agcagcgtgc
      961 cgagaccgaa ggcgacaagc gcaaccagcg cgaaggctga aatgaacttg aagcttttac
     1021 ttgcaggttg ttcaacccgg taaaggcgga tcggcgccgg gtggtaatcg gccttcccgc
     1081 ggatcgcggc ggagagctcg cgctggcgct cgcaggcggc gcgcagcgcc gggttctcgg
     1141 ccatctgcgc ctcgaactcg aggctcgcgg cggcgtcgag ctcgccgtcg aggtaggcgt
     1201 gcagccggtt ctcctccgct ttcatttgtg cacccgcagc cgcggcgggg cggcgatgat
     1261 gcgcagcgct tcggcgagct gacgacgcgc gcgcgacagg cgcgacatca cggtgccgat
     1321 ggggatctcg gcgatgcgcg cgatgtcctt gtaggtgagg tcctcgagct cgcgcagcac
     1381 cagcacctcg cggaactgca ccggcagcgc ggcgatcgct tcgttgaggc ggcgccggtc
     1441 cacatcgcgc gcggcgagcg cctctgggcc gggcgcatcg gattcctgct ccatcccgtc
     1501 gacggcgtgc agctccgcgg ggcggttcgc ctgcagccag ctgaagcagg cgttgcgcac
     1561 gatcgcgagc agccagggcc gcggcgcgcc gcgcagcccg tcgaagaacc ggtaggcgcg
     1621 cagcagcgct tcctgcacta cgtcctcggc gtcatgatcg ttgcgggtga gccagcgcgc
     1681 gagattgaat gccgcgtcga ggtgcggcaa tacctgctgc tcgaatctcg cgcggcgatc
     1741 ggtcaaggcg cgcccatgtt actcccggcc tcaggccacg acgcggatct tgccggtcat
     1801 gcgctcgtgg ccgtcgccgc agaagatatc gcacaggtac acgaagctgc ctgccttgtc
     1861 cggggtaaag cgcagccgcg ccgtctgccc gggaatgatg tcggcgcgga tgttgaggtc
     1921 gggcgcgttg aaccccatga cgacatcccc gctcgtgaac tcgaggacga ccggcacccc
     1981 tttcttcagc gtcacgatgt tcggcgcgta ggtgaacttg cgcgccgcga tcttgacgac
     2041 tttctccttc ggccctgcgg cgagcgacca tgcgccggcc cccgccagga aggcggcgag
     2101 cgcgcccgaa agcagcctgc ggcgcgtcat acctttacca cgggcatttc gctgatcttg
     2161 tacttgaccg gctgcgcctc ggcctcgacc cggcggaagc ccaggccgcg gtagggctgc
     2221 gccggatccc aggcgagcgg cacgcgcttg ggctgcgagc ccgccacggg cagcgggaag
     2281 atcagcgact tggcggcgtg gtgctcgatg tgcgcggtct tgaaatggtg ctcctggtgg
     2341 atgtggccgt agaacaccgt gacgttgcgg tgcggcataa gcagctcgat cgcccggtcg
     2401 ccgtcgcgcg tcgcccagtc ccacgctgga aagagcggaa acagcgggcg gtgcgtgagc
     2461 actacgatgc gggcgtccgc cttctgtctt tcgaggtcgc tcttcagcca cgcgagctgc
     2521 tcgtcgccga tgcgcgccgc cgggtccgag acattatcga gcgcgatgaa atgcacgccc
     2581 ttgtgatcga aagtgtagtg cgtggcgccg aagaactcct tgaacgcctc gccgcggtcg
     2641 agcgagccgt cgtgctcgcc gggcatcagc cggatgttct tatttttgag ggaggaaatg
     2701 agttccttga attttgcaag gcgtttgcgc cgctccgcgg gatcgtcggt cgtatgcgtg
     2761 aggtcgccgg tgaagacgat gaaatcgggc tgggggtcga gcgcgttcac cgccgcgatc
     2821 gccttcggca gcgtgaccag cgcatccggg ttggccgcgc cctgaaagcc ccagtgcgtg
     2881 tcggagagct gcacgaagaa gaactcgtcc tgcgacgtcg cataggcgtc ggcgccgcga
     2941 gcgaggcgcg acgcgaacac cacccctccg acgcctgcga tcctgaggaa gctcctgcgg
     3001 tcgatcatgg gccgtctcct ttttggggta cccatgcaag accggccggg ccgccggttt
     3061 attccctatc atgcgcaaat gaggctgggt ctcttcatga tgccgatgca cccggcgcac
     3121 cgcgacccgg ccgagacgct gcaggaagac cgcgagacgg tgatcctcgc cgaccgcctg
     3181 ggattccacg acgccttcgt cggcgagcac ctcaccgacc gctgcgagaa cgtcaccaac
     3241 agcttcatct tcctcgccac gctgatcgcc gagactaaaa ccatcaagct cggcaccggc
     3301 acctcgaacc tgtcgcacgc ccatcccacg ctcatcgccg cgcacgcggc gatgttcgat
     3361 cacctggcaa aggggcgctt catcttcggc atcagcccgg gggcgctcgc ttccgatgcg
     3421 gaagcgttgg gaatgccgct tcccatggag gaaagaaaca agctcttcgc cgaggcgatc
     3481 gacgtcatcc tcgccatctg ggagcgcgat gcgccctacg acatcgactt gccgggcaac
     3541 cgcttcaagg tctcgaccgc gaagaccctg ctgcccgagc tggggcgcgg gttcatgtac
     3601 aagccctacc agaagccgcg gcccgagatc gtcggcacgg tggtggcgcc gcattcgaag
     3661 ggcgtgatcg cgatgggcga gcgcgacttc catccgatgt cggccaattt tctcctgccc
     3721 cactggctgc cttcgcattg gaaaaactat gccgaaggca agggccggaa aggacaacag
     3781 gccaatcccg ccgactggcg ggtggcgcgg acgattttcg tggcagacga cgacaagacc
     3841 gcgaagcgct acgccctcga ggacccggcg agcccttacc gcttctactg ggagaagctg
     3901 cgcggcaaca tgatccgcgc caggcgccac gtcatcttca agcttcacga gcaggaggac
     3961 gacagcgcgg tcacgctcga gcggctgctc gaccagctgg tgatctgcgg cagcgccaac
     4021 cgggtcgccg accagatcct cgcgctgcac gagcgggcgg gcgacttcgg cgagctggtc
     4081 tacgccggca tggactgggt cgaccccagg ctggcgaggc gttccatgga gctgatggcg
     4141 gaaaaagtcc tgccgcagct ctagtttttc ttctccggag ggcgaaggcc cggcaccatc
     4201 ggcgtcacgg gctttttcgg ctgcttcggc ttcttcttct cgcggttgct gcgctgcatg
     4261 cccttgggca tcggggtttc tcctggaaaa tagccattat gggactgaaa ccgcaggagc
     4321 tggcgcggat cgccgagctg acgctggagc actacgagcg gcgcgcggac gacttctggc
     4381 gcggcacgcg cgatcacgac gtcagccaga acatcgccga gctgctgcgc cacatcgaag
     4441 gcgagccgcc gttccgcatc ctcgacctgg gctgcgggcc ggggcgcgac ctcaaggcgt
     4501 tcacggcgct cggccacatt gcggtaggcc tcgaaggcgc gccgcgcttc gccgccatgg
     4561 cgcgcgaata cagcggctgc gaggtgtggg agcaggattt cctcaagctc gacctgccgc
     4621 cggcgcgctt cgacggcatt ttcgccaatg cggcgctgtt ccacgttccg ggccaggaac
     4681 tgcaccgggt tctggcagag ctgcgcgccg cgctcaagcc gcgcggggtg ctgttcagct
     4741 cgaacccgcg cggccataac gaggaaggct ggaaccgcgg ccgctacggc gcgtaccatg
     4801 acctcggaac ctggcgccgc tacctctcgg ccgcggggtt cgtcgagctc aaccactact
     4861 atcgtccgcc aggcctgccg cgcgagcagc agccctggct ggcgagcgtt tggcgcaggg
     4921 agaagtaaaa gcccccttgc ctgcgcttgc gcgtaggtgc atcctctcgg ccgcagcatc
     4981 tcccgggagg aggttatgaa gaaactcacc gtcgcggcgt ttctcgctgt cgtacccctc
     5041 ttcgcttttg cacagaaagt cgggccgtcg ggcatcggcg gcgtggtcac cgggccgaaa
     5101 gggccggagg cgggcgtgtg ggtgatcgcg gagaccacgg atcttccgac caagtacgcc
     5161 aaggtggtcg tcaccgacga caagggccgc tacctgatcc ccgagctgcc ccaggcgaat
     5221 tacacggtgt gggcgcgcgg ctacggagtg aaggacagcg ccaagtccaa ggcggcgcgg
     5281 ggcgaggcgg tgaatatcaa ggtcgccgcg gccagcgcca aggaggacgc cgagcattat
     5341 cccgggatgt actggtattc catgatcaac atccccgcga aaaatcagtt ccccggcacg
     5401 ggtgagaagg gcaacggcat tagcgacaac atcaagacgc aggagcaatg ggtcgacacg
     5461 gtgaagaacg cctgccagtc gtgccactcg ctcggcagca agggcatgcg cactgtgccg
     5521 aaggagttcg gccccggcgt cccgggctgg gcgcggcgca cgcagtcggg acaggccctg
     5581 acgcagatgg cgctcggcct gggttacatg ggagccgatg cggcgctgaa gaactttgcc
     5641 gactggaccg accgcatcgc ggcgggcgag ctgccgttcg cgaagcccga gcgccccaag
     5701 ggcgtggagc gcaacatggt ggtcaccatg tgggatttct cgacgccgaa atactatctg
     5761 cacgacggca tctcgaccga ccgggacaat ccgcggctca atgccaacgg cccgatctac
     5821 ggcgcgcccg aggagagcac ggacaacatt ccggtgctcg accccgtgaa gcacaaggcc
     5881 cacacgatca agcaccccgt catcaatccc aatacgccat cctcgaccag cctgccgatg
     5941 cagccctcgc cctactgggg caaggagccg atctgggacg gccacagcag catccacaac
     6001 gcgatgatgg atagcgaggg gcgggtatgg ttcaccgcca ggatccggcc gaatgccaac
     6061 ccggacttct gcaagaaagg ctccgatcat ccgtcggcta aggtcgcgcc gctggacgcg
     6121 tcgctgcgcc aggtctccgt gtacgacccg aagacggaaa agtggcggca cgtcgacacc
     6181 tgcttcacca cgcaccacct gtatttcgcc aaggacgcca accggacgct gtggctgagc
     6241 cagggcggcc cggcaagcgg cgtggtcggg tgggtgaaca ccaagctgtt cctcgagacc
     6301 ggcgactcgg caagatcgca gggctggac
//
LOCUS       DASPXW010000144         4256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1307657, whole genome shotgun sequence.
ACCESSION   DASPXW010000144 DASPXW010000000
VERSION     DASPXW010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307657"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..287)
                     /locus_tag="VEL04_03455"
     CDS             complement(18..287)
                     /locus_tag="VEL04_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47452.1"
                     /translation="MRNSEVPMMTLRRAVEWCGGIDALGDALNVQAASLERWIAGEEA
                     VPLEVFKQAVGLLLEASATRPPHSTPDSSDGGRTQKRRGGGGRQH"
     gene            352..1260
                     /locus_tag="VEL04_03460"
     CDS             352..1260
                     /locus_tag="VEL04_03460"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HYT47453.1"
                     /translation="MQDQKLGFIGLGVMGEPMCRNLAAKSRGSSVVAFDLRAEPLARL
                     ATDGVAGARSIAAVAAAADIIFLSLPGEAEIRAVCLGENGRGLVNLARDGQAIVDCST
                     APVALARELAAAFDARGAHFADAPVARTRQAAIDGTLSVMVGADDFLYHRIEPLLRRM
                     ATDVTHCGAPGAGQAVKLLNNMVLSMTVVALAEALAVAKASGVVDPKLLFDTLAKGSA
                     DSFALRNHGMKALLPESHPKDAFPVAYMLKDVSYALELARSAGLDLQGGQTVSRLLAE
                     TQRLGLGEAYHTAVIEAVRRHRPDRA"
     gene            complement(1189..1869)
                     /locus_tag="VEL04_03465"
     CDS             complement(1189..1869)
                     /locus_tag="VEL04_03465"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HYT47454.1"
                     /translation="MKMILAFLLALGAGSAPAQDLTKAVLLVASPELQGLYSQTLLVA
                     VPVDGTHIGFIVNRATDVKLATLFPEHAPSAKVADPIYFGGPEMANSIFAVARRDPGG
                     RSLELFGELFVTASAESIDRIIEQTPNDARYFAGFVGWKPGELEKEVDRGYWYVTDPD
                     SSLFFRSDTSGLWEELVKRLGNGHAPQQGHGQHHARLESGAGSSSPIRAMPAHRLDDR
                     GVVRLAKP"
     gene            complement(1904..2236)
                     /locus_tag="VEL04_03470"
     CDS             complement(1904..2236)
                     /locus_tag="VEL04_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47455.1"
                     /translation="MKALQDRTRDVERHRLQADLDARLEMIFHSCQSLCGFSVELRPI
                     PSTDGEAGVPECELFVSGVDAYPALGGGESEQRLRVEISAALADLLEERPEAAELLPG
                     RTFARVWH"
     gene            complement(2309..3130)
                     /locus_tag="VEL04_03475"
     CDS             complement(2309..3130)
                     /locus_tag="VEL04_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HYT47456.1"
                     /translation="MNSSGQIIRQRRSLLACDGKTSIPAERFYRMLVRVMPCVELDVA
                     QRPMPWDALPWDPAIHFGLFVHRVEGIDPGLYALARDPGKVEKLKRSMHAQFAWQTPP
                     RCPESLPLYLLERGDARQLATQVSCGQAIAGEGAFSLGMIAEYQERLVAHGPWFYRRL
                     FWEAGVIGQVLYLEAEAAGVRATGIGCFFDDPVHQAFGFGDLAFQSLYHFTVGGQVDD
                     PRLTTLPPYGARVTGHSRGGCANSTVAECGKSPPGRPRQRFLTKYCAMGNLPRVA"
     gene            complement(3127..4053)
                     /locus_tag="VEL04_03480"
     CDS             complement(3127..4053)
                     /locus_tag="VEL04_03480"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HYT47457.1"
                     /translation="MPHAAQLEAVMRYHERTKHHFNRFAAGPGGLDWANQPDPFRRYK
                     GAPLTRLPILGAGEEPRSPRYHSLYAPGAVPAAQLSVRALSRLLEYALALSAWKQAGG
                     TRWALRANPSSGNLHPTEGYVLIGGIAELGETPGLFHYAPREHALERRADCPAALFER
                     LMRGFPPQAFLAGLSSVHWREAWKYGERAFRYCQHDIGHAIGTLRIAAATLGWSAAVL
                     DEVADATIEALLGLDRAADFEGAERESAELVMAVWPSAAAKNKAVPLHLDPQPVKELA
                     EQRWYGKANRLSLDDPLPWEIIDAVSAASRKP"
     gene            complement(4065..>4256)
                     /locus_tag="VEL04_03485"
     CDS             complement(4065..>4256)
                     /locus_tag="VEL04_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009769869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYT47458.1"
                     /translation="PDQAKTRGISEEQVVRDVILAAQPTKQFVKVEEVAALAAFLCSE
                     AAAAITGAMLSIDGGWTAE"
BASE COUNT          646 a         1512 c         1442 g          656 t
ORIGIN      
        1 aatgtaacac agttcgctca gtgctgcctg ccgcctcccc cgcggcgctt ctgcgtgcgc
       61 ccgccgtcgg aggaatcggg cgtgctatgc ggcggccgag tcgccgaagc ttcgagcagc
      121 aggccgaccg cctgcttgaa cacctcgagc ggcactgcct cctcgcccgc gatccagcgc
      181 tcgaggctcg cggcctgcac gttgagcgcg tcgccgagcg cgtcgatgcc gccgcaccac
      241 tccaccgcgc ggcgcagggt catcatcggc acctccgagt ttcgcatccc acatcctacc
      301 cggccggcgt gcggttaagc tcctcccagg cgggctgccc gggaggactc catgcaggac
      361 cagaagctcg gtttcatcgg ccttggcgtg atgggagagc ccatgtgccg caacctcgcg
      421 gcgaagtcgc gcggctcttc ggtcgtcgcc ttcgacctgc gcgccgagcc gctcgcgcgc
      481 ctcgccaccg acggcgtcgc cggcgcgcgc tcgatcgccg ccgtcgcagc cgccgccgac
      541 atcatcttcc tctcgctgcc cggcgaagcc gagatccgcg ccgtctgcct gggcgagaat
      601 ggccgcggcc tcgtcaacct tgcgcgcgac gggcaggcga tcgtcgactg cagcaccgcg
      661 ccggtggcgc tcgcgcgcga gctcgccgcc gccttcgacg cgcgcggcgc gcatttcgcg
      721 gatgcgccgg tggcgcgcac gcgccaggcg gcgatcgacg gcaccttgag cgtcatggtc
      781 ggcgccgacg acttcctcta ccaccgcatc gagccgctgc tgcgccgcat ggcgaccgat
      841 gtcacgcact gcggcgcgcc cggcgccggc caggcggtga agctgctcaa caacatggtg
      901 ctgtcgatga cggtggtggc gctcgccgag gcgctcgcgg tcgcgaaggc gagcggcgtg
      961 gtcgatccga agctgctctt cgacacgctc gccaagggct cggccgacag cttcgcgctg
     1021 cgcaaccacg gcatgaaggc actgcttcca gagtcccacc cgaaggacgc cttcccggtc
     1081 gcctacatgc tgaaggacgt gagctacgcg ctcgagctcg cgcgcagcgc gggtctggac
     1141 ttacaggggg gtcagaccgt cagcagactg ctggcggaaa cgcaacggct agggcttggc
     1201 gaggcgtacc acaccgcggt catcgaggcg gtgcgccggc atcgcccgga tcgggcttga
     1261 ggagcccgct ccgctttcga gcctggcgtg gtgctgtccg tgaccctgct gcggggcatg
     1321 accgttgccg aggcgcttga ccagctcttc ccacaacccg ctcgtgtcgc tgcgaaagaa
     1381 gaggctcgag tccgggtcgg tgacgtacca gtagccgcgg tcgacctctt tttcgagctc
     1441 gcccggcttc cagccgacga agcccgcgaa gtagcgtgcg tcgttcggcg tctgctcgat
     1501 gatgcggtcg atgctctcgg cgctcgcggt aacgaacagc tcgccgaaga gctccagcga
     1561 tcggccgccg gggtcgcgcc gcgccacggc aaagatggag ttggccatct ccggcccgcc
     1621 gaagtagatc gggtcggcga ccttcgccga cggcgcgtgt tcgggaaaga gcgtcgccag
     1681 cttcacgtcg gtggcgcggt tgacgatgaa gccgatgtgc gtgccgtcga ccggcaccgc
     1741 caccagcagc gtctggctgt agagaccctg aagctcgggc gaagccacga gcagcacggc
     1801 cttggtgagg tcttgcgcgg gcgccgatcc ggcgccaagc gcgagcagga acgcgaggat
     1861 cattttcatg aaagtcctcc cttgagcagg tcacccgcgc ggctcaatgc cacacccgcg
     1921 cgaacgtccg gccgggcagc aactcggccg cctccgggcg ctcttccaag agatcggcga
     1981 gcgcggctga gatctcgaca cggagccgct gctccgactc gccgccgccg agcgcggggt
     2041 aggcgtcgac gcccgagacg aacagctcgc attccggcac gcccgcttcc ccgtccgtgg
     2101 acgggatcgg gcgcaactcc accgagaaac cgcagagcga ctggcagctg tggaaaatca
     2161 tctcgagccg tgcgtcgaga tccgcctgca agcggtggcg ttccacatcc ctggtccggt
     2221 cctgaagcgc tttcatgggc tccctccgtc gatgtgtgcc gacgcttcac gctaccgaag
     2281 cgctgcgcgc gtgcctagcg acaattcctc aagcgactcg aggtaaatta cccatcgcgc
     2341 aatacttcgt gagaaatcgc tgccgcggcc ggccaggcgg actttttccg cattccgcaa
     2401 ctgttgaatt tgcacagccg ccgcggctgt gtcctgtcac gcgcgcaccg tagggcggca
     2461 gcgtggtgag gcgcggatcg tccacctggc cgccaaccgt gaagtggtag agcgactgga
     2521 aggcaagatc gccgaagccg aaggcctggt gcaccgggtc gtcgaagaag cagccgatgc
     2581 cggtcgcgcg cacgccggcc gcttccgcct cgaggtagag cacctggcct atgacgccgg
     2641 cctcccagaa caggcgccgg tagaaccacg gcccgtgcgc gaccagcctt tcctggtact
     2701 cggcgatcat gccgagcgag aacgcgccct cgccggcgat ggcctggccg cagctcacct
     2761 gcgttgcgag ctgccgcgcg tcaccgcgct cgaggagata gagcgggagg ctctcggggc
     2821 agcgcggtgg tgtctgccag gcgaactgtg cgtgcatgct tcgcttgagc ttctccacct
     2881 tgccgggatc gcgcgcgagc gcgtacaagc ccgggtcgat gccctcgacg cggtgcacga
     2941 acaggccgaa gtggatcgcc gggtcccagg gaagcgcgtc ccagggcatg ggacgctgcg
     3001 ccacgtcaag ctcgacgcac ggcatgacgc gcacgagcat gcggtagaaa cgctctgccg
     3061 ggatcgaagt cttgccgtcg caggcgagca ggctgcgccg ctggcgaatg atctgtccgg
     3121 aagaattcac ggcttgcgcg acgccgccga gacggcgtcg atgatctccc agggcagcgg
     3181 atcgtccagg ctcagccggt tcgctttccc gtaccagcgc tgctccgcaa gttcctttac
     3241 tggttgcgga tccaggtgga gcgggaccgc cttgttcttc gcagcagcgc ttggccatac
     3301 cgccatcacc agctccgcgc tttcgcgctc ggcgccctcg aagtccgcgg cccggtcgag
     3361 gccgagcagc gcctcgatgg tcgcgtccgc cacctcgtcc agcacggccg ccgaccagcc
     3421 gagcgtcgcc gccgcgatcc tcagcgtgcc gatcgcatgg ccgatgtcgt gctggcagta
     3481 gcgaaaggcg cgctcgccgt atttccacgc ctcgcgccag tggacggacg acaggcccgc
     3541 gagaaatgcc tgcgggggaa agccgcgcat caggcgctcg aacagcgccg ccgggcagtc
     3601 ggcgcggcgc tccagcgcat gctccctcgg cgcgtaatgg aacagtcccg gcgtctcgcc
     3661 gagctccgca atgccgccga tcagcacata gccctcggtg ggatgcaggt tgccgctcga
     3721 cggattcgcg cgcagcgccc agcgcgtgcc gcccgcctgc ttccacgccg acagcgcgag
     3781 cgcgtactcg agcaggcgcg acagtgcgcg cacgctcaac tgcgcagccg gcaccgcgcc
     3841 cggcgcgtac aggctgtggt accggggtga gcgcggctcc tcgccggcgc ccaggatcgg
     3901 cagccgtgtg agcggcgcgc ccttgtagcg ccggaacgga tccggctggt ttgcccagtc
     3961 gagcccgccg ggccccgccg caaagcggtt gaagtggtgc ttggtgcgct cgtgataccg
     4021 catcaccgct tcgagctggg cggcgtgcgg cacggaaggg accgttactc cgcggtccag
     4081 ccgccgtcga tggagagcat cgccccggtg atcgccgcgg cggcctccga gcacaggaac
     4141 gcggccagcg ccgcgacctc ctcgaccttg acgaactgtt tggtcggctg cgcggcgagg
     4201 atcacgtcgc gcaccacctg ctcctcgctg atgccgcggg tcttcgcctg gtcggg
//
LOCUS       DASPXW010000145         2683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_925951, whole genome shotgun sequence.
ACCESSION   DASPXW010000145 DASPXW010000000
VERSION     DASPXW010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925951"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VEL04_03490"
     CDS             <1..457
                     /locus_tag="VEL04_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47459.1"
                     /translation="AAIIVLAAAVTFHMDRERPEPDMPVAAAPEPVKTPVSKPSEVER
                     KASRAEAPKRERGTDDARKPGAYGFAPEPPAQTAETQRAAPAPAARSEVRAARLMAQA
                     ESPERTLERIAELRRQGRHEEADQALKEFRKRYPDYKIPEAMLQKVEKK"
     gene            complement(528..1256)
                     /locus_tag="VEL04_03495"
     CDS             complement(528..1256)
                     /locus_tag="VEL04_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HYT47460.1"
                     /translation="MISIKNVSKWYGSFQVLTDCTTEVAKGEVVVVCGPSGSGKSTII
                     KCVNALEPFQKGEIIVEGISVGDAKTNLPKLRARIGMVFQNFELFPHMSVTENLTLGQ
                     IKVLGRSEAAAREKGLKLLDRVGLLAHKDKFPGQLSGGQQQRVAIARALSMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELAREGMTMMVVTHEMGFARKVAHRVIFMDAGRIVEDA
                     KKEEFFGTPRSERAQQFLSKILHH"
     gene            complement(1253..1945)
                     /locus_tag="VEL04_03500"
     CDS             complement(1253..1945)
                     /locus_tag="VEL04_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331911.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HYT47461.1"
                     /translation="MDAGFDFDVIQRSVGYLFREGMTFTVTLTLLAMTGGIIFGTLLA
                     MMRLSAFKPLSWLAGAYVNLMRSIPLVLVIFWFFFLVPYIGAWILREPNPIKVGAFYS
                     ALITFTMFEAAYYCEIMRAGIQSIARGQAWSGYALGLNYWQTMGYIVLPQAFRNMLPV
                     LLTQTIILFQDTSLVYVISATDFLGAASKVANRDYRLVEMYSFVAVVYFIISYSLSTL
                     VKGLQDRIAIVR"
     gene            complement(1967..2668)
                     /locus_tag="VEL04_03505"
     CDS             complement(1967..2668)
                     /locus_tag="VEL04_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HYT47462.1"
                     /translation="MYLKYLVVGLGWTLVTALAAWVIALGVGAAVGTLRTTPLPWVVR
                     LGNLYVEIFRNVPLIVQMFLWFFVAPELLPKGLGDWIKQMPPPWGSYIPAVLCLGIFT
                     SVRVAEQVRAGINSLPRGQRMAGTAMGLTQAQTYRYVILPQAFRIILPPLTSEFMNII
                     KNSSVALTIGLLELTGRARAMQEFSFHVFEAFATATAIYLLTNLVVVLGMRALEQKVR
                     VPGLIAAGGAPMAGH"
BASE COUNT          527 a          837 c          898 g          421 t
ORIGIN      
        1 cgcagcgatc atcgtgcttg ccgccgcggt gacgttccac atggatcgcg agcgtccgga
       61 gccggatatg ccggtcgccg ccgcgcctga gccagtgaaa acccccgtaa gcaagccatc
      121 cgaggtcgaa aggaaggcgt ccagggctga agcgccgaag cgggaacgtg gaacggacga
      181 cgcgcgcaaa ccgggcgcat acggctttgc gcccgagccc ccggcgcaga cggcggaaac
      241 ccagcgtgcc gcgcctgcgc cggccgcgcg gtcggaggtg cgcgccgcga ggctgatggc
      301 gcaggccgaa tcacctgaac gcacgctcga gcgcatcgct gagctgcgca gacagggcag
      361 gcacgaagag gcggaccagg cgttgaagga atttcgcaag cgctatccgg attacaagat
      421 tccggaagcc atgctgcaga aagtcgagaa aaaatagaaa aaatagggac agaccccatt
      481 tttctcatcg cgaagctaac gaaaaatggg gtctgtccct atttttccta gtggtgcagg
      541 atcttggaga ggaactgctg cgcgcgctcg gagcggggcg tgccgaagaa ctcctccttc
      601 ttcgcgtcct cgacgatgcg gccagcgtcc atgaagatca cgcggtgcgc caccttgcgc
      661 gcgaagccca tctcatgcgt taccaccatc atggtcatgc cttccctggc gagctcgacc
      721 atgacgtcga gcacctcgtt gatcatctcg gggtcgaggg cggaggtggg ctcgtcgaac
      781 agcatcgcga tcgggtccat cgacaacgcg cgcgcgatcg cgacgcgctg ctgctggccg
      841 cccgagagct ggcccgggaa cttgtccttg tgggcgagga ggccgacgcg gtcgaggagc
      901 ttcaggcctt tctcgcgcgc cgcggcttcg ctccgaccga gcaccttgat ctggccgagg
      961 gtcaggtttt cggtgacgct catgtgcggg aacagctcga agttctggaa caccatgccg
     1021 atgcgcgcgc gcagcttggg caggttggtc ttcgcatcgc ccaccgagat gccctcgacg
     1081 atgatctcgc ccttctggaa cggctcgagc gcgttgacgc acttgatgat ggtcgacttg
     1141 ccgctgccgg aggggccgca gacgacgacg acttcgccct tggcgacctc ggtggtgcag
     1201 tcggtgagca cctggaagct gccgtaccac ttgctgacgt tcttgattga gatcatcgga
     1261 cgatggcgat cctatcctgg aggcccttga cgagcgtgga caagctgtac gagatgatga
     1321 agtagaccac cgcgacgaag ctgtacatct cgaccaggcg gtagtcgcgg ttcgccacct
     1381 tggaggcggc gccgaggaaa tcggtcgccg agatcacgta caccagcgag gtgtcctgga
     1441 acaggatgat ggtctgcgtg agcagcaccg gcagcatgtt gcggaacgcc tgcggcagca
     1501 cgatgtagcc catggtttgc cagtagttca gccccagcgc gtagcccgac cacgcctggc
     1561 cgcgcgcaat cgactggatg ccggcgcgca tgatctcgca gtagtacgcc gcctcgaaca
     1621 tggtgaaagt gatgagcgcg gaatagaacg cgccgacctt gatcggattg ggctcgcgca
     1681 ggatccaggc gccgatgtag ggcaccagga agaagaacca gaagatcacc agcaccagcg
     1741 ggatcgagcg catcaggttg acgtacgcgc cggcgagcca ggagagcggc ttgaacgccg
     1801 agagccgcat catggcgagc agcgtgccga agatgatgcc gccggtcatg gcgagcagcg
     1861 tgagcgtgac ggtgaaggtc atgccctccc tgaagaggta gccgaccgag cgctggatga
     1921 cgtcgaaatc gaacccggca tccatcgttc aatgccccgc ctgcatttag tgtcctgcca
     1981 tgggcgcgcc gccggccgcg atcaggcccg gcacgcgcac tttctgctcg agcgcgcgca
     2041 ttcccagcac caccaccagg ttggtgagca ggtagatcgc cgtcgcggtg gcgaaggcct
     2101 cgaacacgtg gaagctgaac tcctgcatcg cccgcgcccg gccggtgagc tcgagcaggc
     2161 cgatggtcag cgccaccgag gagttcttga tgatgttcat gaactcggaa gtgagcggcg
     2221 gcaggatgat gcggaacgcc tgcggcagga tgacgtagcg gtaggtctgc gcctgggtga
     2281 gccccatggc ggtgcccgcc atgcgctggc cgcgcggcag cgagttgatg ccggcgcgca
     2341 cctgctcggc gacgcgcacc gaggtgaaga ttcccaggca cagcaccgcc ggaatatacg
     2401 agccccacgg cggcggcatc tgcttgatcc agtcgccgag gcccttcggc agcaactccg
     2461 gcgccacgaa gaaccacagg aacatctgca cgatcagcgg cacattgcgg aagatctcga
     2521 cgtacagatt gccgagccgc accacccacg gcagcggcgt ggtgcgcagc gtgcccaccg
     2581 ccgcgccgac gcccagggcg atgacccagg cggcgagcgc ggtgaccagc gtccagccga
     2641 ggcctacgac cagatacttg agatagatcc cggtggcgcc ggg
//
LOCUS       DASPXW010000146         2747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_295948, whole genome shotgun sequence.
ACCESSION   DASPXW010000146 DASPXW010000000
VERSION     DASPXW010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295948"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /gene="alr"
                     /locus_tag="VEL04_03510"
     CDS             <1..893
                     /gene="alr"
                     /locus_tag="VEL04_03510"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HYT47463.1"
                     /translation="FQEAERLRLAGVAKPILMLEGFFKPADIALIHKYALTPVIHNPE
                     QVEMLRLTVLPGEIDVYVKVNSGMNRLGFTVDNLRAAYNAIRMHAQVRQVTLMTHFAD
                     ADGASGVAGQLEWFGDLTKDYEAPRSLANSAALIRFPETRGNWVRPGIMLYGCSPFAD
                     RSAEALELRPAMTLTSEIIATQHLQPGERIGYGFGYEAVGEITIGVVACGYADGYPRH
                     APVGTPVLVNGQRARIVGRVSMDMICVDISDIPEAYIGTPVTLWGEGLSADEVAAAAG
                     TLSYELLCALAARVPVSEVP"
     gene            904..2244
                     /gene="radA"
                     /locus_tag="VEL04_03515"
     CDS             904..2244
                     /gene="radA"
                     /locus_tag="VEL04_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006053000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HYT47464.1"
                     /translation="MTKAKSAYVCSECGAPALQWFGSCPSCGAAGTLSEALTERGSHR
                     FHAAPVTLSTVETRELERLPTGIGELDRALGGGLVPGQVALLGGDPGIGKSTLLLQTI
                     SSIEPAEKCLYVTGEESADQIALRARRLSIDARAVRLLAETQLERVLTALDSARPRVA
                     VIDSIQTLWSETLQSAPGSVAQVRECAAQLTRHAKRAGTALFIIGHVTKEGAIAGPRV
                     LEHIVDTVLYFEGDPQSSFRLVRAVKNRFGAVNELGVFAMTDKGLKGVANPSRLFLST
                     HEKPVSGSCVLATLEGTRPLLVEIQALVDSAHAPNPRRLSVGLEQNRLAMLLAVLHRH
                     AGIATWEQDVFVNAVGGVRIAEPAADLAVSLAIVSSLSDKPIAAKLVVFGEIGLAGEV
                     RPAPRGQDRLREAAKLGFEKAVIPKSNLPKGKIAGLEIFAVERVDAAVQLLRTL"
     gene            complement(2223..2627)
                     /locus_tag="VEL04_03520"
     CDS             complement(2223..2627)
                     /locus_tag="VEL04_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47465.1"
                     /translation="MSSKLVLKNPRLPELEALEVEALADSGAVHLCIPEQVRRRLGLE
                     AIYEQEITLADGSTRRVPYVGPIEIRFKNRVGFAGALVLGDQVLVGAIPMEDMDLVVM
                     PRTRTLDVNPNSPNIATSIVKQSPAAYRVRRS"
BASE COUNT          470 a          908 c          910 g          459 t
ORIGIN      
        1 atttccagga agccgagcgc ctgcggctcg ccggcgtcgc caagccgatc ctcatgctcg
       61 agggcttctt caagccggcg gacatcgcgc tcatccacaa atatgctctc acgcccgtga
      121 ttcacaatcc ggagcaggtc gagatgctgc ggctcaccgt gctgccgggc gagatcgacg
      181 tctacgtgaa ggtgaacagc ggcatgaacc gcctcggctt caccgtcgac aacctgcgcg
      241 ccgcatacaa cgcgatccgc atgcatgccc aggtgcggca ggtcacgctt atgacgcact
      301 tcgccgatgc cgatggtgcc agcggggtgg cgggccagct tgaatggttc ggtgacctga
      361 caaaagatta cgaagcgcca cgctcgctcg cgaattcggc ggcactgatc cgcttcccgg
      421 aaacgcgcgg caactgggtg cgtccgggaa tcatgctcta tggctgctcg ccgttcgcgg
      481 atcgcagcgc ggaggcgctc gagctcaggc cggcgatgac gctgacctcg gagatcatcg
      541 cgacgcagca cctgcagccg ggcgagcgca tcggctacgg cttcggctac gaggcggtgg
      601 gcgagatcac catcggtgtg gtcgcttgcg gctacgccga cggctatccg cgccacgcgc
      661 cggtcggcac gccggtgctc gtcaacggcc agcgcgcccg catcgttggc cgggtatcga
      721 tggacatgat ctgcgtcgac atcagcgaca tccccgaggc ctacatcggc acgccggtca
      781 cgctctgggg cgagggactt tcggcggacg aggtcgcggc ggcggcgggg acgctcagct
      841 acgagctgct gtgcgccctc gcagcccgcg tgccggtcag cgaagttcca tgagtcgaaa
      901 aaaatgacaa aggcaaaaag cgcctatgtc tgctccgagt gcggcgcccc ggcgctgcaa
      961 tggttcggca gctgcccgtc gtgcggcgcc gcgggaacgc tgagcgaggc gctcacagag
     1021 agaggctctc atcgcttcca cgccgcgccg gtcacgctga gcacggtcga gaccagggag
     1081 ctcgagcgcc ttccgaccgg catcggcgag ctcgaccgcg cgctcggcgg cgggctggtg
     1141 ccggggcagg tggcgctgct cggcggtgat ccgggaatcg gcaagtcgac cctgctttta
     1201 caaaccatat cttccattga acctgcagag aaatgcctct atgtgaccgg agaggaatcg
     1261 gcagaccaga tcgcgctgcg cgcgcggcgc ctgtcgatcg acgcccgcgc ggtgcggctg
     1321 ctcgccgaga cccagctcga gcgcgtgctc acggcgctcg actcggcgag gccgcgcgtc
     1381 gcggtgatcg actcgatcca gacgctttgg tccgagacgc tgcagtcggc gcccggatcg
     1441 gtggcgcagg tgcgcgagtg cgcggcgcag ctcacgcgcc acgcgaagcg cgccggcacg
     1501 gcgctcttca tcatcggcca tgtcaccaag gagggcgcca tcgccgggcc gcgcgtgctc
     1561 gagcacatcg tcgacacggt gctctacttc gagggcgacc cgcagtcgag cttccgcctg
     1621 gtgcgtgcgg tgaagaaccg cttcggcgcg gtgaacgagc tcggcgtctt cgccatgacc
     1681 gacaaggggt tgaagggcgt agccaatccg tcccgccttt tcctctccac tcatgaaaag
     1741 ccggtgtccg gctcgtgcgt gctcgcgacg ctcgagggca cgcggccgct gctggtggaa
     1801 atccaggcgc tggtcgacag cgcgcacgcg cccaacccgc gccggctgtc ggtgggcctg
     1861 gagcaaaacc ggctcgccat gctgctcgcg gtgctgcacc gccacgcggg catcgccacc
     1921 tgggaacagg acgtgttcgt caacgccgtg ggcggcgtgc gcatcgccga gcccgcggcc
     1981 gaccttgcgg tctccctggc aatcgtttcg tcgctgtccg acaagccgat cgcggcgaag
     2041 ctggtggtgt tcggcgaaat cggcctcgcc ggcgaagtgc gcccggcgcc acgcgggcag
     2101 gaccggctca gggaagccgc aaagctggga ttcgaaaagg cagtcattcc aaaatcaaat
     2161 cttcccaaag ggaaaatcgc agggttggag atcttcgcgg tggagcgcgt cgacgccgcg
     2221 gttcagctcc tgcgcactct gtaggcggct gggctctgct tgacgatcga cgtggcgatg
     2281 ttcgggctgt ttggattaac gtcgagagtg cgcgtgcgcg gcatgaccac caggtccatg
     2341 tcttccatag gtatggcgcc gacgagaacc tggtcgccaa gcaccagcgc gcccgcaaag
     2401 ccgacgcggt tcttgaagcg tatttcgatc ggcccgacgt aaggaacccg tctcgtcgag
     2461 ccgtcggcga gcgtgatttc ctgctcgtag atcgcttcca ggcccaagcg ccgccgaacc
     2521 tgctccggga tgcacagatg cacggcgccc gagtccgcca gggcttcgac ctccagcgcc
     2581 tcgagctccg gcaatcgcgg atttttcagg acgagcttgc tgctcacaag gcccatttta
     2641 tcctcctcga tgacactaac gcgcggttgt cctcccagac gagagacaag tcatatgatc
     2701 gaaatcatat gcccccctgg tgctcggcat taggctgcgc catcaaa
//
LOCUS       DASPXW010000147         2441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_422965, whole genome shotgun sequence.
ACCESSION   DASPXW010000147 DASPXW010000000
VERSION     DASPXW010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422965"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2113
                     /gene="ligD"
                     /locus_tag="VEL04_03525"
     CDS             <1..2113
                     /gene="ligD"
                     /locus_tag="VEL04_03525"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HYT47466.1"
                     /translation="TERNRSVLSGVTVEEMTAVPVQRIPAERLVPGGEKRPLPEKLSP
                     MHAEIGDAVFNSPDWMWEPKLDGYRALAFIDEEGVRLRSRRGLELGEEFPRLVAELGK
                     QAVNGMVLDGEIVAFDASGKPSFGAMQNRGGGVERAVFYCFDLLYFAGVDLRKTTYAE
                     RRRYLAQCLLPSPLVQLVHAEDDGLALHNAAMASGFEGVIGKRKNSAYEAGKRSAAWL
                     KVKAINSADFVVGGYTKGKGSRAPLGALLLGYWQGKKLRFASHVGSGFDDKTLARTRK
                     LLEPLQRKTSPFADEPELNAPTSWVEPELVAEVKFQGWTDDGHLRAPVFLRLRDDVDP
                     KEVRRAQADATPAVEGDDILRQLENTKTAFTLAIGKDRIRLTHLDRVYWPADPALKQP
                     ALTKRDLLRYFAQVSPYILPHLADRPLTMIRMPDGIHGQRFFQKHWEQERPPFVETIT
                     VFSGHKDEKHDYLLCNNLPTLLWLAQSGTLEFHVWHSRAKPGPDAAGKRTDYASSLAA
                     LESSVLNYPDYVVFDIDPYIYSGKEAAGEEPELNTVAFEKGKEVAFWLRELLQSLSME
                     SIVKTSGKTGLHVFVPILRSVDFDAARQVSEIVGRHLMRRHPKDITLEWSVPKRTGKI
                     FMDYNMNVRGKTLNVAYSPRGAAGAPVSMPLTWDELAKAHPLDFRLTNAAERLARTGD
                     RWRDALTRKQSLERTLERPKA"
     gene            2180..>2441
                     /locus_tag="VEL04_03530"
     CDS             2180..>2441
                     /locus_tag="VEL04_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HYT47467.1"
                     /translation="MAARSIASLTISFGMVSIPVKLYSATEASRAISFNLLHKSCGSR
                     LKQQYFCVKEEVPVARDDMVKGYEFAKEQYVMFSPEELKAMEE"
BASE COUNT          453 a          770 c          798 g          420 t
ORIGIN      
        1 gaccgagcgg aatcgctctg tgctctccgg tgtcacggtg gaggagatga ccgccgtgcc
       61 ggtgcagcgc atacccgccg agcggctcgt ccccggcggc gaaaagcggc cactacccga
      121 gaaactctcg ccgatgcacg cggagatcgg cgatgcggtt ttcaacagcc ccgactggat
      181 gtgggagccg aagctcgacg gctatcgcgc gctcgctttc attgacgagg aaggcgtgag
      241 gctgcgctcg cggcgagggc tggagctcgg cgaggaattc ccgcgcttgg tcgccgagct
      301 cggcaagcag gcggtgaacg gcatggtgct cgacggcgag attgtcgctt tcgacgcgag
      361 tggcaagcct tcgttcggcg cgatgcagaa ccgcggcggt ggcgtcgagc gcgcggtttt
      421 ctactgcttc gatctgctgt acttcgccgg cgtcgacctg cgcaagacga cctatgccga
      481 gcggcggcgc tacctcgcgc aatgccttct gccgtcgcct ctggtgcagc tcgtccacgc
      541 cgaagacgac ggccttgccc tgcacaacgc ggcgatggcg agcggcttcg agggcgtgat
      601 cggcaagcga aagaacagcg cctacgaggc gggcaagcgc tcggcggcgt ggttgaaggt
      661 gaaggcgatc aacagcgccg acttcgtcgt cggcggctac acgaagggga aagggtcgcg
      721 cgcgccgctc ggcgcgctgt tgcttggcta ctggcagggc aagaagctgc gtttcgcctc
      781 gcacgtcggc tccgggttcg atgacaagac gctggcgcgg accagaaaac tgctcgagcc
      841 gctgcagcgc aagaccagtc cgttcgccga cgagccggaa ctgaacgcac cgacttcctg
      901 ggtcgagcct gagctggtcg ccgaagtgaa gttccagggc tggacagacg acgggcatct
      961 gcgagccccg gtctttctcc ggctgcgcga tgacgtcgac ccgaaagagg tgcggcgtgc
     1021 gcaggcggat gccacgccgg ccgtggaagg cgatgacatc ctgcgccagc tggagaacac
     1081 gaagaccgcc ttcaccctcg cgatcgggaa ggaccggatc cgcctcaccc atctggaccg
     1141 cgtgtactgg ccggcggacc cggcgctcaa gcagccggcg ctgaccaagc gcgacctgct
     1201 gcgttatttc gcgcaagtct cgccctacat cctgccgcat ctcgcggacc gcccgctcac
     1261 catgatccgc atgcccgacg gcatccacgg gcagcgtttt ttccagaaac actgggagca
     1321 ggagcggccc cctttcgtcg agaccatcac cgtcttctcg gggcacaagg acgagaagca
     1381 cgactatctg ctgtgcaaca acctgccgac gcttctgtgg ctcgcccagt ccgggacgct
     1441 cgaatttcac gtctggcatt cacgcgcgaa gccgggtccg gacgccgccg gcaagcgcac
     1501 cgactacgcc agctccctgg ccgcgctgga aagctcggtg ctcaactatc ccgactacgt
     1561 cgtcttcgac atcgatccct acatctattc gggaaaggaa gcggccggcg aggagcctga
     1621 gctgaacacg gtcgctttcg agaagggtaa ggaggttgcg ttctggctgc gtgagctgct
     1681 gcaaagccta tcgatggaaa gtattgtcaa gacttcgggc aagaccggcc tgcacgtctt
     1741 cgtgccgatc ctgcgcagcg tggatttcga cgccgcgcgc caggtgtcgg agatcgtcgg
     1801 gcggcacctg atgcgccggc atcccaagga catcacattg gagtggagcg tgcccaagcg
     1861 gacgggaaag atcttcatgg actacaacat gaacgtgcgc ggcaagaccc tcaacgtcgc
     1921 ctactcgccg cgtggcgccg ctggcgcgcc ggtgtcgatg ccgctgactt gggacgagct
     1981 cgcgaaggcc cacccccttg acttcaggct taccaacgcg gccgagcggc ttgcccgcac
     2041 cggcgatcgg tggcgcgacg cgctcacgcg caagcaaagc ctggaacgca cccttgagcg
     2101 ccccaaggca taatgcggta tgcttcgaac ctttcgctcg aagtacgtcg cttaagcaat
     2161 tgattttaag aggtgaatca tggctgcgcg atcgattgca tcgttgacca tctccttcgg
     2221 gatggtttcc ataccggtga agctctattc ggccaccgag gcgagccgcg cgatttcgtt
     2281 caacttgctg cacaagagtt gcggctcgcg cctcaagcag cagtacttct gcgtcaagga
     2341 ggaagtgccg gtggcgcgcg acgacatggt gaagggctac gaattcgcca aggagcagta
     2401 cgtgatgttc tcccccgagg agctcaaggc gatggaggag g
//
LOCUS       DASPXW010000148         3631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_170665, whole genome shotgun sequence.
ACCESSION   DASPXW010000148 DASPXW010000000
VERSION     DASPXW010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170665"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VEL04_03535"
     CDS             complement(<1..267)
                     /locus_tag="VEL04_03535"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HYT47468.1"
                     /translation="MPPVTQALLVANVVIFLAEMSGAVSLGEFALWPPGGFESRFEPW
                     QLVTYAFLHANLAHIFFNMLALYMFGAEVERLFGARFYAAYYFAS"
     gene            complement(271..1227)
                     /locus_tag="VEL04_03540"
     CDS             complement(271..1227)
                     /locus_tag="VEL04_03540"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYT47469.1"
                     /translation="MIPRFLALLAACAFSIQALAQPYPSKPIKIVVGFPPGGSGDFLT
                     RLIADEMQKALGVAVIADNRPGAGGNLAFENVAKSAADGYTVLNAPHPAVNLALYKNP
                     GYAEKDFVPVTKIATGPCVIVVNNSVPINNLRELIAYAKANPGKLFNASAGFGSAPHL
                     AAVLFQSVAGVQFTQVQYKGGGPAAQSVLAGDTQIAFSTAPTVMGFVRAGRLRPLGVT
                     MKKGSPSVPGIPGSEEAGLPDYEYTFWFGLFVPAGTPVPVVRRLHEAAVKSLAKPEMR
                     DRIAIQGMDPTPSASPEAFEADIRAEAPALERMIRESGARVE"
     gene            complement(1224..2036)
                     /locus_tag="VEL04_03545"
     CDS             complement(1224..2036)
                     /locus_tag="VEL04_03545"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HYT47470.1"
                     /translation="MIDVQGHRGARGYLPENTLPAFARALGMGVDTLELDCGVTRDGV
                     VVVHHDRRLNPDIARGPDGKWVAAPAPTITSLTFEELQRYDVGRLRPGSEYAKRFPHQ
                     KPIDGTRIPRLAALFDLARNSGVRFNIEPKSDLEAPQDTLPPEPFAKALLAEIRKASL
                     EKRTTVQAFDWRSLKVVEREAPEMGTVYCTEGDNSDPAKVHAAGGRIWSPNFAELSER
                     KLAEARKLKMRITVWTVNQPADIASMIDAGVDGMASDYPDRVIELLKKRAKR"
     gene            complement(2033..2401)
                     /locus_tag="VEL04_03550"
     CDS             complement(2033..2401)
                     /locus_tag="VEL04_03550"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HYT47471.1"
                     /translation="MWNTGLVAHLGIEFVEVGRDKVVATLKVRGELLTTTDTVHGGTL
                     MAFADTIGAAGTVANLMPGQNTATLESKTNFFAACRSGTLKAESVPLHRGKRTHVWQT
                     RVTDDAGKLLSLTIQTQMIL"
     gene            complement(2412..2750)
                     /locus_tag="VEL04_03555"
     CDS             complement(2412..2750)
                     /locus_tag="VEL04_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HYT47472.1"
                     /translation="MTEAKYFLVVRFWVAPEAVAQVSRWLEGGHVAEVVSQPGFLWCR
                     RLNLKEKDAKGWSAFSMLYGIESRHAFERYESDQELKSKFARQREPFEKLMRIERFSG
                     EVDFALDNGG"
     gene            complement(2747..>3631)
                     /locus_tag="VEL04_03560"
     CDS             complement(2747..>3631)
                     /locus_tag="VEL04_03560"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYT47473.1"
                     /translation="LLVLHGCAGVLWSPAAQLFIHDIVDDDRLHSAIRLTATARWLGL
                     LMGPAVGGVILLLMGPAGGILFNALIYLPFIVWLWKAPWQRKVMPADRRVRAFADVVS
                     TWRAVAGNRVIASMTVLVGGASLIVGNAYQAQMPEYARDLGHPDGGITYSMLLAADAA
                     GALVAGLALESRGLLPPDPRTAYLLAMLWCVAIGCFAAATSYPLALLLLFAAGFLELA
                     FFAMAQTLVQTNAPPQVRGRVIGLFAMSALGLRAFSGVTVGLGGSLIGIHFSLALSAA
                     ALLILAVGLFVLTGRKAA"
BASE COUNT          610 a         1242 c         1186 g          593 t
ORIGIN      
        1 gctcgcgaag tagtacgcgg catagaatcg cgcgccgaac agccgctcga cctcggcgcc
       61 gaacatgtac agcgccagca tgttgaagaa aatgtgcgcc agattggcgt gcaggaaggc
      121 gtaagtgacg agctgccagg gctcgaagcg cgactcgaag ccgcccggcg gccacagcgc
      181 gaactcgccg agcgacaccg cgcccgacat ctccgcgagg aagatgacga cgttcgcgac
      241 cagcagcgcc tgcgtgaccg ggggcaccgg ctactccact ctggcgccgg attcgcggat
      301 catgcgctcc agcgctggcg cctcggcccg gatgtcggcc tcgaaagctt cgggcgacgc
      361 cgacggcgtc ggatccattc cctggatcgc gatcctgtcg cgcatctcgg gcttggcgag
      421 gcttttgacc gccgcttcgt gcaggcgccg caccaccggc accggcgtgc ccgcgggcac
      481 gaagagcccg aaccagaacg tgtactcgta gtccggcagg cccgcttcct cgcttcccgg
      541 gatcccgggc accgagggcg agcccttctt catggtcacg ccaagcggcc gcagccgccc
      601 ggcgcgcacg aagcccatca ccgtcggcgc ggtggaaaag gcgatctgcg tgtcgcccgc
      661 gagcaccgac tgcgccgccg gcccgccgcc cttgtactgc acctgcgtga actgtacgcc
      721 ggcgaccgac tggaacagca ccgccgcgag gtgcggcgcc gagccgaagc ctgcggaggc
      781 gttgaacagc ttgccgggat tcgccttggc gtacgcaatc agctcgcgca ggttgttgat
      841 gggcacgctg ttgttcacca cgattacgca cgggccggtg gcgatcttgg tcaccgggac
      901 gaaatccttc tctgcatagc ccgggttctt gtagagcgcg aggttcaccg cgggatgcgg
      961 cgcgttcagc acggtgtagc cgtcggccgc gctcttcgcc acgttctcaa aggcgagatt
     1021 gccgcccgcg cccgggcggt tgtccgcaat caccgccacg ccgagcgcct tctgcatctc
     1081 gtcggcgatc aaccgggtga ggaagtcgcc cgagccgccg ggcgggaagc cgaccacgat
     1141 cttgatgggt ttcgacggat acggctgcgc gagcgcctgg atcgaaaaag cgcacgccgc
     1201 cagcaatgca agaaacctgg gaatcatcgc tttgctctct tcttcaaaag ctcgatcacg
     1261 cggtccggat aatcgctcgc catgccgtcg accccggcgt cgatcatgct cgctatgtcg
     1321 gccggctggt tgaccgtcca gacggtgatc ctcattttca gcttcctggc ttcggcaagc
     1381 ttgcgctccg atagttcggc aaaattcggc gaccagatcc tgccgcctgc ggcatgcacc
     1441 ttcgcggggt ccgagttgtc tccctcggtg caatacacgg tccccatttc cggcgcctcg
     1501 cgctcgacca ccttcaggct gcgccaatcg aacgcctgga cggtggtgcg cttctcgagg
     1561 ctcgccttgc ggatctcggc gagcagcgcc ttcgcgaaag gctcgggcgg cagcgtgtcc
     1621 tggggcgctt cgaggtcgct cttcggctcg atgttgaatc tgacgccgct gttccgtgct
     1681 aaatcgaaaa gcgccgccag gcgcggaatc ctcgtgccgt cgatcggctt ctggtgggga
     1741 aagcgcttcg cgtactcgct ccccggccgc aggcgcccga cgtcgtagcg ctgcagctcc
     1801 tcgaatgtga gggaggtgat cgtgggcgcc ggcgccgcga cccatttacc gtcggggccg
     1861 cgtgcgatat cgggattgag cctgcggtcg tggtgcacca ccaccacgcc gtcgcgcgtc
     1921 acgccgcagt cgagctcgag cgtgtcgact cccatcccca gcgctcgggc gaacgccggc
     1981 agcgtgttct cgggcaggta gccgcgtgcg ccgcgatgtc cctggacgtc gatcacagga
     2041 tcatttgcgt ctggatcgtg agcgaaagca gcttgccggc gtcgtcggtg acacgcgtct
     2101 gccagacgtg ggtgcgcttg ccccggtgca gcggcacgga ttccgccttg agcgtgcccg
     2161 agcggcaagc ggcgaagaag ttggtcttcg attcgagcgt cgcagtgttc tggcccggca
     2221 tcaggttcgc caccgtgcct gccgcgccga tggtgtcggc gaacgccatc agcgtgccgc
     2281 cgtgcacggt gtccgtcgtc gtaagaagct cgcccctgac cttgagcgtc gccaccacct
     2341 tgtcgcggcc gacctcgacg aactcgatgc cgaggtgcgc cacgagtccc gtattccaca
     2401 tgtccacggc tctagcctcc gttgtccagc gcgaaatcaa cctcacccga gaagcgctcg
     2461 atgcgcatca gcttttcgaa cggctcgcgc tggcgcgcga atttgctttt cagttcttga
     2521 tccgattcat atctttcaaa ggcgtggcgc gattcgattc catacagcat ggaaaacgcg
     2581 ctccagccct tcgcgtcctt ttctttcagg ttcaacctgc ggcaccagag gaatccgggc
     2641 tggctcacca cttcggcgac atgcccaccc tcgagccagc gcgaaacctg cgccacggct
     2701 tccggggcga cccagaagcg caccacgaga aagtatttcg cctcggtcac gccgccttcc
     2761 ttccggtgag cacgaacagc ccgacggcga gtataagcag cgccgcggcg ctcagggcga
     2821 gcgagaagtg gatgccgatc aggctgccgc cgagccccac ggtgacgccg ctgaaggcgc
     2881 gcagccccag cgccgacatg gcgaacaggc cgatgacgcg gccgcggacc tggggcgggg
     2941 cgttggtctg caccagcgtc tgggccatgg cgaaaaacgc gagctcgagg aagccggcgg
     3001 cgaacagcag cagcagggcg agcgggtagg aggtcgcggc tgcgaaacag ccgatcgcca
     3061 cgcaccacag catcgccagc agatacgcag tgcggggatc gggaggcagc aggccgcggc
     3121 tttcgagcgc cagtccggcg accagcgctc cggccgcgtc cgcggcgagc agcatgctgt
     3181 aggtgatgcc gccgtccggg tgcccgaggt cgcgcgcata ttcgggcatc tgcgcctggt
     3241 aggcgttgcc gacgatgagc gaggcgccgc cgaccagcac cgtcatcgag gcgatgacgc
     3301 ggtttccggc caccgcgcgc caggtcgaca cgacgtcggc aaacgcgcgc acacgacggt
     3361 cggcaggcat caccttgcgc tgccagggcg ccttccacag ccagacgatg aagggcaggt
     3421 agatgagcgc gttgaagaga atcccccctg ccggacccat cagcagcagg atcacgccgc
     3481 ccactgccgg acccatcagc agccccagcc agcgcgccgt cgccgtgagc cggattgcgc
     3541 tatgcaggcg gtcgtcatcc acgatgtcgt ggatgaagag ctgcgccgcg gggctccaca
     3601 gcacgcccgc gcagccgtgc agcacgagca g
//
LOCUS       DASPXW010000149         2599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1055459, whole genome shotgun sequence.
ACCESSION   DASPXW010000149 DASPXW010000000
VERSION     DASPXW010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055459"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="VEL04_03565"
     CDS             complement(<1..593)
                     /locus_tag="VEL04_03565"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYT47474.1"
                     /translation="MIEDLYRARTPRSAALYAEACRVLPAGLTHDSRMTSPYPIYVAR
                     AAGARKWDVDGNEYVDYFGGHGALLFGHSHPPLVEAVQRQAALGTHWGASHELELRWA
                     EAVQRLVPSAQRVRFTGSGTEASQLALRLARAYTGKSKIVRFVGHFHGWHDAVVPGGM
                     SHYDGSVPAGISASLAGESLLLPADDAGPVLDVLQTR"
     gene            632..1681
                     /locus_tag="VEL04_03570"
     CDS             632..1681
                     /locus_tag="VEL04_03570"
                     /inference="COORDINATES: protein motif:HMM:NF037995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HYT47475.1"
                     /translation="MLTRTLGAIAIGSLFSFSASAQDLPKTQFKVIGLNSPTVASSVD
                     EVPFWRETLPKASNGAITADITPLDQMGIDDKTMLRLLRLGVMDFAGMDISKMAGDDP
                     RFEGCDLAGITLDTDRARAGCEAYRAVIDRQMQKNWNARLLAFGGNPPQVFWCRGMLG
                     GLNDLKGKKVRVFNNTMRDFLGGVGATAVSMAFAEVVPALNNGVVDCGVTGSLSGNTA
                     GWPEVTKSLYPMSLGWSINVMAVNLASWNRLDKRVQDFMLREVKAYENKMWGTLKKAT
                     AEADNCNTGKQPCTMGKLANITIVPVKPAEAAMHKKLVEGAVLAGWAKRCGAECAAEW
                     NNTVGKTLGLRAPTN"
     gene            1735..2244
                     /locus_tag="VEL04_03575"
     CDS             1735..2244
                     /locus_tag="VEL04_03575"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HYT47476.1"
                     /translation="MLAAALLIGVDVTLRKFFNASIGGADELAGYALALGTAWSLGAA
                     LLDRAHIRIDSLYVLFPRWLRLALDFAGLALFIAFFGLIARHGWSVAQQSWVSGSRSQ
                     SALATPTVIPQSVWLAGLAFFFVIGVALLAHAVILIARGNATAAAQAISTRSAVEEVE
                     EEIRDLKGG"
     gene            2245..>2599
                     /locus_tag="VEL04_03580"
     CDS             2245..>2599
                     /locus_tag="VEL04_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HYT47477.1"
                     /translation="MLWTALVILLALLALAVPVAAGLGLLGIALSDIYSRLPLTLALG
                     EMAWATSNNFLLVAIPFFVLLGEILLRSGMAERMYNALVLWVPWLPGGLMHSNIAACA
                     MFAATSGSSVATAATI"
BASE COUNT          416 a          868 c          897 g          418 t
ORIGIN      
        1 tcgcgcgtct gcaacacatc gagcaccggg cctgcgtcat ccgcgggcag cagcagcgac
       61 tcaccggcga gcgaggccga aatgccggcc ggaaccgagc cgtcgtaatg cgacatgcct
      121 cccggcacca cggcgtcgtg ccagccgtga aagtggccga cgaagcgcac gatcttcgac
      181 ttgccggtgt aagcgcgcgc aaggcgcagt gcgagctgcg aagcctcggt ccccgagccg
      241 gtgaagcgca cgcgctgcgc cgacggcacg agccgctgca cagcctcggc ccagcgcagc
      301 tcgagctcgt gcgaggcgcc ccagtgcgtg ccgagcgccg cctggcgctg cactgcctcg
      361 acgagcggcg gatgagaatg gccgaagagc agggcgccgt gcccgccgaa gtagtcgacg
      421 tactcgttgc cgtcgacgtc ccacttgcgt gcaccagcgg cgcgcgcgac gtagatcgga
      481 tagggcgaag tcatccgcga gtcgtgcgtg agccctgccg gcagcacgcg gcaggcctcg
      541 gcatagagcg cggcggagcg tggcgtgcga gcccggtaca ggtcttcaat catgcggtgt
      601 actatgtttc gcaaggagga ggagaccgcc aatgctaact cgcacgctcg gtgcaatcgc
      661 aatcggttca ctcttttcgt tcagcgcctc ggcgcaggac ctgccgaaaa cgcagttcaa
      721 ggtcatcggc ctgaacagcc cgacggtagc ctcgagcgtg gatgaggtgc cgttctggcg
      781 cgaaacgctg cccaaggcgt cgaacggcgc gatcaccgcg gacatcacgc cgctcgacca
      841 gatgggcatc gacgacaaga cgatgctgcg gctcctgcgc ctcggggtga tggacttcgc
      901 cggcatggac atctccaaga tggcgggcga cgacccgcgc ttcgaaggct gcgacctggc
      961 gggcatcacc ctcgatacgg acagggcgcg cgccgggtgc gaggcttacc gcgcggtgat
     1021 cgaccgccag atgcagaaaa actggaacgc gagactgctc gccttcggcg gcaacccgcc
     1081 gcaggtattc tggtgccgcg gcatgctcgg cggcctaaac gacctcaagg gcaagaaggt
     1141 tcgcgtgttc aacaacacca tgcgcgactt cctgggcggc gtcggggcga cggcggtcag
     1201 catggcgttc gccgaggtgg tcccggcgct caacaacggc gtggtcgact gcggcgtcac
     1261 cggcagcctc tccgggaaca ccgcgggctg gcccgaggtg acgaaatcgc tttacccgat
     1321 gtcgctcggc tggagcatca acgtgatggc ggtgaacctg gcgagctgga accggctcga
     1381 caagcgcgtg caggacttca tgctgcgcga ggtgaaggcc tacgagaaca agatgtgggg
     1441 cacgctgaag aaggcgaccg ccgaggccga caactgcaac accggaaaac agccctgcac
     1501 catgggcaag ctcgccaaca tcaccatcgt gccggtgaag ccggccgagg ccgcgatgca
     1561 caagaagctc gtcgagggcg cggtgctcgc cggctgggcg aagcgctgcg gcgccgagtg
     1621 cgccgcggag tggaataaca ccgtcggcaa gaccctgggc cttagggcac ccacgaactg
     1681 attgccttcg cgcagcgcct gacgcgcttc gggctgtggt tcagcggcgc gctggtgctc
     1741 gccgccgccc tgctgatcgg cgtcgatgtc acgctgcgca agttcttcaa tgcgtcgatc
     1801 ggcggcgccg acgagctcgc cggctatgcg ctggcgctgg gtactgcgtg gtcgctcggc
     1861 gctgcgctgc tcgaccgcgc gcacatccgc atcgactcgc tgtacgtgct gttcccgcgc
     1921 tggctgcggc tcgcgctcga tttcgccggg cttgccctct tcatcgcctt cttcggcctg
     1981 atcgcgcgcc acggctggag cgtggcgcag cagtcgtggg tgtcggggtc gcgcagccag
     2041 tcggcgctcg ccacgccgac ggtgatcccg cagtcggtat ggctcgcggg ccttgcgttc
     2101 tttttcgtca ttggcgttgc gctgcttgcg catgctgtga tcctgatcgc gcgcggcaac
     2161 gcgacagcgg cggcacaggc catcagcacc cgctccgcgg tcgaggaggt cgaggaggaa
     2221 atccgcgacc tgaagggcgg atagatgctc tggacggcgc tcgtgatcct gctcgcgctt
     2281 ctcgcgctcg ccgtgccggt tgcggcgggg ctcggcctcc tcggtatagc gctctcggac
     2341 atctactcgc ggctgccgct cacgctcgcg ctgggcgaga tggcgtgggc gacgtcgaac
     2401 aacttcctgc tcgtggccat cccgttcttc gtgctgctcg gcgagatcct gctgcgctcg
     2461 ggcatggccg agcgcatgta caacgcgctg gtgctgtggg tgccgtggct gcccggcggc
     2521 ctgatgcact ccaacatcgc cgcctgcgcg atgttcgccg ccacctcggg ctcgagcgtc
     2581 gccaccgcgg ccaccatcg
//
LOCUS       DASPXW010000150         3628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_675588, whole genome shotgun sequence.
ACCESSION   DASPXW010000150 DASPXW010000000
VERSION     DASPXW010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675588"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1129)
                     /locus_tag="VEL04_03585"
     CDS             complement(<1..1129)
                     /locus_tag="VEL04_03585"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HYT47478.1"
                     /translation="MPGPLHGVRVLDLTTVVMGPYATQILGDFGADVVKLEPPEGDVI
                     RNAWPFRNPGMGTIFLNTNRNKRSIVLDLKKDLAREACLALAAKADVLVYNIRPQAMA
                     RLRLSYEEVRKANPRIIYVGCFGFSQRGPYAAKAAYDDMIQGAAGIPWLLKKQGASEP
                     RYAPMIIADRSVGQQVASAVSAALYHREKTGKGQRIDVPMFEHLLQMILGDHLGGYTF
                     EPPHGEPGYIRILSPDRRPYQTKDGYVCALIYNDKQWKGFFEVIGKPQVLSQPEFSSH
                     EARSRNYDRAYAMVAEELKKRTTAQWVEAFERADIPVQRMNSLDDIVADPHLAAIGYL
                     RTVEHPTEGRIKSLAVPSEWSESAPEYRRHAPRLGEHTREVL"
     gene            complement(1129..2862)
                     /gene="ptsP"
                     /locus_tag="VEL04_03590"
     CDS             complement(1129..2862)
                     /gene="ptsP"
                     /locus_tag="VEL04_03590"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HYT47479.1"
                     /translation="MNFVLHGHAVSGGITVGYAHLVSTARLEVAHYEVAEDAVEAEVA
                     RFDRAMKLAQDELYELKAHIAADAPAEFAAFLDLHRMILNDAALAQVPRELIRSRRSN
                     AEWALVQQMERLVERFDEIDDPYLRERKADVQQAVERVLKAMLGGQTITEPALTEEQK
                     LIVVAHDLSPADMMLFKRHHFGGFVTDVGGVTSHTAIVARSLGIPAIVGLHHARQTVS
                     EGELLIVDGVQGLLVVNPDPLVLAEYRSRQSQLKAERQKLKRLKKTPAATLDGTPVEL
                     YANIELPQDTSDAIEAGAEGVGLFRTEFMFLNRKDLPSEDEQFEAYRQVAQAMQGRPV
                     VLRTLDVGADKALNGGDGSHIMPNPAMGLRAIRFCLAEPQMFLTQLRAILRASHYGKV
                     RILLPMIAHAHEIDQTLALVAQAKEQLDERGLPYDRGTEIGGMIEIPAAALALPIFMR
                     RLNFLSIGTNDLIQYTLAIDRTDDAVAHLYDPLHPAVLTLVAGIIQTATRGGVPVAVC
                     GEMAGDLQLTRLLLGLGLRNFSMHPSQLLPTKERILHTNLAEVQAGAQRVLRNSDPDK
                     TRELLAQLNAE"
     gene            complement(2859..3128)
                     /locus_tag="VEL04_03595"
     CDS             complement(2859..3128)
                     /locus_tag="VEL04_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HYT47480.1"
                     /translation="MPSVCAEIVNKLGLHARAAAKLTHTASGFQSEIWLSRSGRRVNA
                     KSIMGVMMLAAGQGTAVLIEAQGSDAEPAIAAILKLIADKFGEGE"
     gene            complement(3121..3525)
                     /locus_tag="VEL04_03600"
     CDS             complement(3121..3525)
                     /locus_tag="VEL04_03600"
                     /inference="COORDINATES: protein motif:HMM:NF015567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HYT47481.1"
                     /translation="MIGILIVTHGEIGTSMLSSASQILGDAQQQVATLSVWRHDDPDD
                     LVLRARELLERIDSGEGVLVLTDIFGATPGNVVSRLLEDGRVEGVSGVSLPMLLRVLT
                     SRNGSVTAAVQRALSGGAEGVVHMNTDRCCDA"
BASE COUNT          544 a         1208 c         1247 g          629 t
ORIGIN      
        1 gcaatacctc gcgcgtgtgc tcgcccaggc gcggcgcgtg acggcgatac tccggcgccg
       61 actcggacca ctccgagggc acagcgagcg acttgatgcg gccttcggtc ggatgctcca
      121 cggtgcgcag gtagccgatc gcggcgaggt gcgggtcggc gacgatgtcg tcgaggctgt
      181 tcatgcgctg caccgggatg tcggcacgct cgaacgcctc gacccactgc gcggtagtgc
      241 gcttcttcag ctcctcggcc accatggcgt aggcgcggtc gtagttccgg ctcctcgcct
      301 cgtgcgacga gaactcgggc tgggacagga cctgcggctt gccgatgacc tcgaagaacc
      361 ccttccattg cttgtcgttg taaatcagcg cgcacacgta gccgtccttg gtctggtacg
      421 gccggcggtc cggcgacagg atgcggatgt agcccggctc gccgtgcggc ggctcgaagg
      481 tgtagccgcc caggtgatcg cccaggatca tctgcagcag gtgctcgaac atcggcacgt
      541 cgatgcgctg gcccttgccg gtcttctcgc ggtgatacag cgccgcgctc accgcgctcg
      601 ccacctgctg gccgaccgag cggtcggcga tgatcatcgg cgcgtagcgc ggctcgctcg
      661 ccccctgctt cttcagcagc caggggatcc cggcggcgcc ctggatcatg tcgtcgtagg
      721 cggccttggc cgcgtagggg ccgcgctggc tgaagccgaa gcagccgaca taaatgatcc
      781 ggggattggc tttccttact tcttcatacg aaagccgaag gcgggccatg gcctgcgggc
      841 ggatgttgta gaccaggacg tccgctttcg ccgccagcgc gaggcaggct tcgcgcgcca
      901 gatccttctt gaggtcgagg acgatcgagc gcttgttgcg gttggtgttg aggaagatgg
      961 ttcccatgcc cggattcctg aacggccagg cattgcggat cacgtcgcct tcgggcggct
     1021 cgagcttcac cacgtcggcg ccgaagtcgc cgaggatctg ggtggcgtac ggccccatca
     1081 ccaccgtggt gagatcgagc acgcgaacgc cgtgcagggg gccgggcatt attccgcgtt
     1141 cagctgggcg agcagctcgc gcgtcttgtc cgggtcgctg ttgcgcagca cgcgctgcgc
     1201 gcccgcctgc acctcggcga ggttggtgtg caggatgcgc tccttggtcg gcaggagctg
     1261 tgacgggtgc atcgagaagt tgcgcaagcc gaggccgagc agcaggcgcg tgagctgcag
     1321 gtcgcccgcc atctcgccgc acaccgccac cggcacgccg ccgcgggtcg cggtctggat
     1381 gatgccggcg accagcgtca gcaccgccgg gtgcagcgga tcgtagaggt gcgccaccgc
     1441 gtcgtcggtg cgatcgatgg cgagcgtgta ctggatcagg tcattggtgc cgatggagag
     1501 aaaattcagc cgccgcatga agatcggcag cgcgagcgcc gcggccggga tctcgatcat
     1561 gccgccgatc tcggtgccgc ggtcgtaagg cagcccgcgc tcgtcgagct gctctttcgc
     1621 ctgagcgacc agcgccagcg tctggtcgat ctcgtgtgca tgcgcgatca tcggcagcag
     1681 gatgcgcact ttgccgtaat gcgaggcgcg caggatcgcg cgcagctggg tgaggaacat
     1741 ctgcggctcg gcgaggcaga agcggatcgc gcgcaggccc atcgccgggt tcggcatgat
     1801 gtgcgagccg tcgccgccgt tcagcgcctt gtcggcgccg acgtcgagcg tgcgcagcac
     1861 caccgggcgg ccctgcatcg cctgcgccac ctgccggtag gcttcgaact gctcatcttc
     1921 cgacggcagg tccttgcggt tgaggaacat gaattcggtg cgaaacagcc ccacgccttc
     1981 ggcaccggct tcgatcgcat cggaagtatc ctgcggcagc tcgatgttgg cgtacagctc
     2041 gaccggcgtg ccgtcgagcg tggccgcggg cgtcttcttc aggcgcttga gcttctgccg
     2101 ctcggccttg agctgagact ggcggctgcg atactcggcg agcaccagcg gatcgggatt
     2161 gacgacgagc agcccctgca cgccgtcgac gatcaggagc tcgccctcgc tgacggtctg
     2221 gcgcgcgtgg tgcaggccga cgatcgccgg gatccccagg ctgcgcgcaa cgatcgcggt
     2281 gtgcgaggtg acgccgccga catcggtgac gaagccgccg aaatggtggc gcttgaacag
     2341 catcatgtcg gcgggcgaca aatcgtgcgc caccacgatc agcttctgct cctcggtgag
     2401 cgccggctcg gtgatcgtct gtccgcccag cattgccttc agcacccgct cgacggcctg
     2461 ctgcacgtcg gccttgcgct cgcgcaggta cgggtcgtcg atctcgtcga agcgctcgac
     2521 caggcgctcc atctgctgca ccagcgccca ttcggcattc gagcgccggc tgcggatcag
     2581 ctcgcgcggc acctgcgcca gcgccgcgtc gttgaggatc atgcggtgca ggtcaaggaa
     2641 agccgcgaac tcggcgggcg cgtcggccgc gatgtgggct ttcagctcgt agagctcgtc
     2701 ctgcgcgagc ttcatggcgc ggtcgaagcg cgccacctcg gcctcgaccg cgtcctcggc
     2761 gacctcgtag tgcgcgacct cgagccgcgc ggtcgagacg agatgggcgt aaccgaccgt
     2821 gatgccgccc gacacggcat gaccatgcag gacgaaattc attctccttc gccgaattta
     2881 tccgcaatca gtttcaggat tgcggcaatc gccggctcgg catcgctgcc ttgggcttcg
     2941 atcagcaccg cggtgccctg gccggcggcg agcatcatca cgcccatgat gctcttcgcg
     3001 ttgacccggc ggccgctgcg cgagagccag atctcgctct gaaagccgct tgccgtgtgc
     3061 gtgagcttcg ccgcggcgcg cgcatgcagg ccgagcttgt tcacgatctc ggcgcaaacg
     3121 ctaggcatcg cagcagcggt cggtgttcat gtgcaccacg ccctcggcgc cgcccgagag
     3181 ggcacgctgc accgcggcgg tgaccgagcc gttacggctg gtgagcaccc gcagcagcat
     3241 cggcagcgac actcccgaga cgccttcgac gcgaccatcc tcgagcagcc gcgacaccac
     3301 gttgcccggg gtggcgccga agatgtcggt cagcaccagg acgccctcgc cggagtcgat
     3361 tctctcgagc agctcgcgcg cgcgcagcac caggtcgtcg gggtcgtcgt gccgccagac
     3421 gctgagggtc gcgacttgct gctgcgcgtc gccgaggatc tggctggcgc tcgagagcat
     3481 cgaggtgccg atctcgccgt gggtgacgat caggatgccg atcacgggcc gctcacccca
     3541 gcttcgattc gagcgcgtcc atgaacatgc ccggcacgtc gaatccggtc tggtcctgga
     3601 tctcgcggaa gcaggtcggg ctggtgac
//
LOCUS       DASPXW010000151         2860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_676505, whole genome shotgun sequence.
ACCESSION   DASPXW010000151 DASPXW010000000
VERSION     DASPXW010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676505"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..646)
                     /locus_tag="VEL04_03605"
     CDS             complement(56..646)
                     /locus_tag="VEL04_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012399797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYT47482.1"
                     /translation="MAQDSSKPEFWDTRFRGGVTPWDAGSVPSALEIWLKKRSSSLRV
                     LIPGCGTGYEVRAFAERGHSVLAIDFSDAALDAARRELGKLASLVRRDDFFSLREKPF
                     DVVYERAFLCALPRSLWTEWARRTAELVRPGGELAGFFYLDDNQRGPPFGIARSQLNT
                     LLTTNFQLSEETPIPAEQSLPVFKGKEVWQVWGRRL"
     gene            645..1316
                     /locus_tag="VEL04_03610"
     CDS             645..1316
                     /locus_tag="VEL04_03610"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYT47483.1"
                     /translation="MGRFSLALACFLPLGVFAQGASPQVNEPYRNPSYADWQRRLETE
                     GREVYERRHAIVAATGLRPGQAVADVGAGTGLFTRLFAAQVGSQGHVYAVDIAREFVH
                     GNLHRAREEGYANVTGIESTQADTRLPERSVDVAFICDAYHHFEQPQAMLASIRRALR
                     PGGTLVLIDYERVPGVSEDWILKHIRAGKEEFRREIEAAGFRFVEEVKLMRESYFLRF
                     ARDPG"
     gene            1326..1562
                     /locus_tag="VEL04_03615"
     CDS             1326..1562
                     /locus_tag="VEL04_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47484.1"
                     /translation="MRVIGSRTAYRVRAHPSGAALKAGAAHQAVAASLAAVPTTGVAR
                     GIYRFASHAEANAHSEEAQARAIAQNLRARHLIP"
     gene            1570..2058
                     /locus_tag="VEL04_03620"
     CDS             1570..2058
                     /locus_tag="VEL04_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47485.1"
                     /translation="MPRDARPATAEDLKQLLSALHREGVEYLLIGGYALFALGYQRGT
                     VDIDLLLPRSRDTGERLKRALLALPDKVARDLDPAWFLDDETIRVADEFVVDLMFNAC
                     GETYDSLLPYAVTIDFDGIPVRTLNLEGLLKTKQSERERDRFDRLAIERALDAAKKGK
                     AP"
     gene            complement(2077..>2860)
                     /locus_tag="VEL04_03625"
     CDS             complement(2077..>2860)
                     /locus_tag="VEL04_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016344218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HYT47486.1"
                     /translation="VEELRRQGVDIPKLARTGVEIFFTQVFRDGFFHADMHPGNILVT
                     PEGRYVALDFGIMGTLTDVDKNYLAQNFLAFFDRDYRRVAAAHLEAGWVPAGTRVDEF
                     ETAIRAVCEPVFARPLKEIYFGKLLLRLFQTSRRFNVQIQPQLVLLQKTLLNIEGLGR
                     ELDPDLDLWRTAKPYLERWMGEQIGWRGLLRTLRHEAPYWAATLPQLPRLVHRALSGD
                     RLGELRQALERLADEQARRDRLMIFSITALLAALIVLAVATL"
BASE COUNT          523 a          951 c          927 g          459 t
ORIGIN      
        1 cgcaatggtg atgaggaggt aggcgatgac cgaagcgatc agcatgcgcg cgccatcata
       61 gccgccgccc ccacacctgc caaacttcct tgcccttgaa gacgggcagc gactgctccg
      121 ccgggatcgg cgtctcctcg ctcaactgaa aattcgtcgt caacagtgta ttcaattgcg
      181 accgcgcaat gccgaatggc ggacctcgtt ggttgtcgtc aaggtagaag aacccggcga
      241 gttcgccgcc cgggcgcacc agctcggcgg tccgccgcgc ccactcggtc cagagggagc
      301 gcggcagcgc gcacaggaag gcgcgctcgt agacgacatc gaagggcttt tcccgcaggc
      361 tgaagaaatc gtccctcctg acgaggctcg cgagcttgcc caactcgcgc ctcgcggcgt
      421 cgagcgcggc atccgagaaa tcgatcgcca agacgctgtg tccgcgctcg gcgaaagcgc
      481 gcacttcgta cccggtgccg caaccaggga tgagcacgcg caaagaagaa cttctctttt
      541 tcagccagat ttccagggcg gacggcacgc tgcccgcatc ccatggcgtg acaccgccgc
      601 gaaagcgcgt gtcccagaac tcgggcttgg agctatcctg cgccatgggc cgattcagcc
      661 tcgcactcgc atgcttcttg ccgctagggg tgtttgcaca aggtgcatcc ccgcaagtta
      721 atgaacctta ccgcaatccg agttacgccg attggcagcg ccgcctcgag accgaggggc
      781 gcgaggtcta cgagcggcgc cacgcgatcg ttgccgcgac cgggcttcgt cccggacagg
      841 cggtcgccga cgtcggcgcg ggaacgggcc tgttcacgcg gctgttcgcc gcgcaggtcg
      901 gcagccaagg tcacgtctat gcggtcgaca tcgcacgcga gttcgtgcac ggcaacctgc
      961 accgcgcgcg cgaggaaggc tacgcgaacg tcaccggcat cgagtcgacc caggcggaca
     1021 ccaggcttcc cgagcgctcg gtggacgtgg cgttcatctg cgacgcgtat caccacttcg
     1081 agcagccgca ggcgatgctc gcctcgatcc ggcgcgcgct gcggccggga ggcacgctcg
     1141 tactcatcga ctacgagcgc gtgcccggcg tgagcgagga ctggatcctc aagcacatcc
     1201 gcgccggcaa ggaagagttc aggcgcgaga tcgaggccgc gggcttccgc ttcgtcgaag
     1261 aggtgaagct gatgcgcgag agctactttc tgcgcttcgc gcgcgatccg ggataaaatt
     1321 cggtcatgcg ggtgatcggg tcccggacgg catatcgcgt gcgcgcgcat ccttctggcg
     1381 cggccctcaa ggcgggcgcc gcgcaccagg ctgtcgcagc atcgcttgcc gcggtgccga
     1441 ccaccggcgt cgcccgaggc atctaccgat tcgcttcgca cgccgaggcg aacgcgcata
     1501 gcgaagaggc tcaagcccgc gcgatcgcac agaatctgcg cgcgcggcac ctgattccct
     1561 gagcccgcaa tgccccgcga tgcgcgcccg gcgaccgcgg aggatctcaa acagctttta
     1621 agcgcgctcc atcgggaggg tgtcgagtac ctcctgatcg gcggctacgc cctttttgcg
     1681 ctcggctacc agcgcgggac ggtcgacatc gatcttctct tgccgcgctc gcgcgacacg
     1741 ggcgagcgtt tgaagcgcgc attgctcgcg ttaccggaca aggtcgccag ggatctcgac
     1801 ccggcatggt ttctcgacga cgagacgatt cgcgtcgccg atgaattcgt cgtcgaccta
     1861 atgttcaatg catgcggcga aacctacgac tcgctgctgc cttatgcggt caccatcgac
     1921 ttcgacggca ttccggtgcg cacgctcaat ctagagggac tgctgaagac caaacagtcc
     1981 gagcgcgagc gggaccgctt cgatcggctc gcgatcgagc gagcgctcga cgcagcaaaa
     2041 aaaggaaaag ctccctgatc gagtccagcg tcagatctaa agcgttgcta cggcgagcac
     2101 gatgagcgcc gcaagcagcg ccgtgatcga aaaaatcatt aaccggtcgc gccgcgcctg
     2161 ttcgtcggcg agccgctcga gcgcctggcg cagctcgcca aggcgatccc ccgagagcgc
     2221 gcggtgcacc aggcgcggca gctgcggcag cgtcgcagcc cagtacggcg cctcgtggcg
     2281 cagcgtgcgc agcaggccgc gccagccgat ctgctcgccc atccagcgct cgaggtaggg
     2341 cttcgccgtg cgccacaggt cgaggtccgg atcgagctcg cgccccaggc cttcgatgtt
     2401 gagcagcgtc ttctgcagca gcacgagctg cggctggatc tgcacattga agcgccgcga
     2461 ggtctggaac aggcgcagca gcagcttgcc gaaatagatc tccttgagcg ggcgcgcgaa
     2521 caccggctcg cacacggcgc ggatcgcggt ctcgaactcg tcgacgcgcg tgccggcagg
     2581 cacccagccc gcctcgagat gcgcagccgc gacgcggcgg tagtcgcggt cgaagaaggc
     2641 aagaaaattc tgtgccagat agttcttgtc gacatcggtg agcgtgccca tgatgccgaa
     2701 gtcgagcgcg acatagcggc cctcgggcgt gacgaggatg tttcccgggt gcatgtcggc
     2761 gtgaaagaac ccgtcgcgga acacctgggt gaagaaaatc tcgaccccgg tgcgcgcgag
     2821 cttcgggatg tccacgccct gccggcgcag ctcctcgact
//
LOCUS       DASPXW010000152         5230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_550898, whole genome shotgun sequence.
ACCESSION   DASPXW010000152 DASPXW010000000
VERSION     DASPXW010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5230
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550898"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VEL04_03630"
     CDS             <1..366
                     /locus_tag="VEL04_03630"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HYT47487.1"
                     /translation="RAFEEIRRQKPDALFVARGATTYAFRHQIGEFASSMRLASACPG
                     ITEFTEAGCLMAYGGNTFEVFRRAASHVSRILNGAKPGDLPIERPNIFELTINLRTAR
                     VLGIAVPPALRARADRVIE"
     gene            381..1343
                     /locus_tag="VEL04_03635"
     CDS             381..1343
                     /locus_tag="VEL04_03635"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYT47488.1"
                     /translation="MTRLAVAAAAWPLVTRAQPPKRIPLIGFLGAESATGYRTHIEAF
                     RAGLRDVGYVEGKNIAIEFRWAEAKYDRLPELAAQLVRRNVDVLVTHGAPGTRAAKGA
                     TTTIPIVMASAGDAVATDIVGNLARPDGNITGSTFFALELVGKRLDVLKQAMPRIAEV
                     AVLFSPDNPITKPVLLEFETAAKSLKLGLQLFPVRERSELDGTLATIAKRRVDALVVP
                     EYSMFVASARAISRLALEHRLPTMGFSEFAEAGGLLGYGYDGADLFRRAAVFVDRILK
                     GAKPGELPVERATKFQLIVNRKTARAIGVPLADNFLLRADKVIE"
     gene            1340..2308
                     /locus_tag="VEL04_03640"
     CDS             1340..2308
                     /locus_tag="VEL04_03640"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYT47489.1"
                     /translation="MKRREFLLAAGALAAPLGVRAQSSRVPRIGVIIHSNAWAPIVEG
                     LRAGLKELGVAEGKDVALEVFDAKADAKAIEETARRFERERVRLIYCVPTSAATVVKR
                     ATTEVPIFFGVGTDPIASGLVESFAKPGGRLTGVHFLSTDLTAKRLELLKDLVPKLRR
                     VVILYRPGHPPAEASARMAREAGEKLGVQVIERHVRSVPEVRAALAALKAGEADADFQ
                     VSDALVSSQFQYLIGASKKIRMPMMVYDQAVVERGALVSYGVDTREVGRQSAKNVQRM
                     LAGARPAELPVETVTRLAFVLNRRTAQEIGLAVPPAMLVRFDRVIE"
     gene            2329..3090
                     /gene="soxA"
                     /locus_tag="VEL04_03645"
     CDS             2329..3090
                     /gene="soxA"
                     /locus_tag="VEL04_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009277238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HYT47490.1"
                     /translation="MVPALAAADEGLESTVRQYREALRDASPGELWVVTGEELFRTPR
                     GPKNASLEACDFGLGPGVVKGAYAQLPRYFADTDRVEDLESRLMSCMRQLQGFGEEAF
                     PRHARGDRRMLDFERLAAYVAAQSNGMPLAAPLAHPKEVDAQRIGERLFYRRAGTHDF
                     SCATCHTLQGKRIRLQRLAQLTDPKEAQGVFPSWPAYRVAAGEVHTLQDWIAICYWAA
                     RHPQLRYGSEASVALQVFMARYANGGVISAPGVKR"
     gene            3087..3686
                     /gene="soxX"
                     /locus_tag="VEL04_03650"
     CDS             3087..3686
                     /gene="soxX"
                     /locus_tag="VEL04_03650"
                     /inference="COORDINATES: protein motif:HMM:TIGR04485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HYT47491.1"
                     /translation="MKRLAFAVLLSMGASPLIGQDDLAQRAEAAFVSSFAGGDPAMLA
                     RTGVQDEVQRLCSEHRNSPPVALAQAIERTQRALIRYPASLIGDWREGKRIAEDFTGS
                     RYDDKAGAPNGGNCYACHQLAPQELAFGTVGPSLLAYGKSRGWGVAAQQRVYDRIYNS
                     QAFVACSAMPRFGHNGVLTPEQIAHLVALLLDPTSEVNR"
     gene            3683..4663
                     /locus_tag="VEL04_03655"
     CDS             3683..4663
                     /locus_tag="VEL04_03655"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYT47492.1"
                     /translation="MTRRRAFLALAAASLCVPETLLAQAKPRRIGYLSPHSITEPPSP
                     ERADFLAGLRELGYEPGRNLVIVYRSADGDFERLPELAAELVRQRVELIVVNSGEAAI
                     AAKAATSTLPLIISAAGDPVQTGLIKSYARPGGNITGLSFIAPELGAKRLALAKEILP
                     QARRVAVIWNARDPASNNEWAQVRDAGPKLGIATDALPVGETDDLARALDLLPKSASQ
                     ALLVIVDARMVGFRKIVADAAIQARIACFAGWREYVTSGALASYAPDFRALFRRAAYY
                     VDRILRGAKPADLPVEQPTKFELVVNLRTAKAIGVTVPNAVLLRADEVIQ"
     gene            4660..>5230
                     /locus_tag="VEL04_03660"
     CDS             4660..>5230
                     /locus_tag="VEL04_03660"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYT47493.1"
                     /translation="MKRRAFLLAAGALAAPFAARAQSAGLPRIGVLIHSTNYAPVVEG
                     LRAGLKELGVAEGKDVALEVFDAKGEAKAIEEAARRFERERVRLIYCTPTSAATVVKR
                     ATSEVPIFFCVGTDPIASGLVESFAKPGGRLTGVHYLTTDLTAKRLELLKELVPKLRR
                     VVTLYRPGHPPAEASARLAREAGKKLGVQV"
BASE COUNT          830 a         1752 c         1817 g          831 t
ORIGIN      
        1 cgcgcattcg aggaaatccg caggcagaag cccgatgcgc ttttcgtcgc gcgcggcgcc
       61 accacctacg ccttccggca ccagatcggc gagtttgcct cttccatgcg tctggcaagc
      121 gcctgtcccg gcatcacgga gttcaccgaa gcgggctgcc tcatggccta cggcggtaat
      181 accttcgagg tcttccgccg cgccgcgagc cacgtgagcc gcattctcaa cggcgcgaaa
      241 cccggtgacc tgccgatcga gcggcccaac atcttcgagc tgacgattaa cctgcgcacc
      301 gcgcgggtac tcggcatcgc ggtgccgccg gcgctgcgcg cccgcgccga ccgggtgatc
      361 gaatgaggcg gcgcgatttc atgactcgtc tcgccgtcgc ggcggccgcc tggccgctcg
      421 tgacgcgcgc acagccgccg aaaagaattc cgctgatcgg gttcttgggg gccgaatccg
      481 ctaccggcta cagaacccat atcgaggcat ttcgggcggg gctgcgtgat gtcggctatg
      541 tcgaaggcaa gaacatcgcg atcgagttcc ggtgggccga ggcgaagtac gaccgcctcc
      601 ccgagctggc agcgcagctc gttcgccgca atgttgatgt tctggtcacg catggagcac
      661 ccgggacgcg tgccgctaag ggcgcgacca ccacgattcc aattgtcatg gccagcgcag
      721 gcgacgccgt cgccacggat atcgtcggaa atctggcgcg gcctgacggg aacatcaccg
      781 gctctacgtt tttcgccctg gagctcgtgg ggaaacggct tgacgtgctc aagcaagcca
      841 tgcctcggat tgccgaggtg gcggtgctct tcagtccgga caatcccata accaaaccgg
      901 tattgctgga attcgagact gccgccaaat ccctgaagct ggggcttcag cttttcccgg
      961 tccgtgagcg gagcgagctg gacggtaccc ttgcgacgat tgcgaagcgg cgcgtcgatg
     1021 cgctcgtggt tccggaatat tcgatgttcg ttgcgagcgc cagggcgata tcgcgtctcg
     1081 cgctggagca ccggcttccc acgatgggat tcagcgagtt cgctgaagcc ggcggtttgc
     1141 ttggctacgg ttacgacggc gccgacctgt ttcgccgcgc cgcggttttc gtcgacagaa
     1201 tcctcaaggg cgccaagccc ggtgagctgc cggtggaacg agccacgaaa ttccagctga
     1261 tcgtgaatcg gaaaaccgcc agggcaatag gggttccgct tgccgacaat tttctgctgc
     1321 gcgccgacaa ggtgatcgaa tgaagcgccg ggaattcctg ctggcggccg gagcgctcgc
     1381 cgcaccgctc ggcgtccggg cccagtcaag ccgcgtgccg cggatcgggg tgatcatcca
     1441 cagcaatgcc tgggccccga tcgttgaagg gctgcgcgcg ggactgaagg agctgggagt
     1501 cgcggaggga aaagacgtgg cgctggaggt cttcgatgcg aaggccgacg cgaaggcgat
     1561 agaagaaacc gctcgccgct tcgagcgcga gcgggtgcgg ctcatctatt gcgtcccgac
     1621 gtcggccgcc accgtcgtga agcgggcgac caccgaggtc ccgatcttct ttggcgtggg
     1681 taccgacccg atcgcgagcg gcctcgtcga gagcttcgcc aagcccggag ggcggctgac
     1741 gggcgtgcat ttcttgagca cggacctgac ggcgaagcgg ctcgagctgc tgaaagacct
     1801 agtgccgaag ctgcgccgcg tggtgatcct ttaccgcccg ggccaccccc ccgccgaggc
     1861 atcagcgcgg atggcccggg aggccggcga gaagctcggg gtccaggtca tcgagcggca
     1921 cgtgcgctcc gtgcccgagg tccgggccgc gctcgccgcg ctcaaggcgg gcgaggcgga
     1981 cgccgatttc caagtctcgg atgccctcgt tagcagccag ttccagtatc tgataggggc
     2041 gtcgaagaag atccgcatgc ccatgatggt ctacgaccag gccgtggtgg aacgcggcgc
     2101 cctggtgagc tacggcgtcg acacccgcga ggtcgggcgc cagtcggcga aaaacgtgca
     2161 gcgcatgctc gccggcgcgc gacccgccga gctgccggtc gaaaccgtga cgcggctcgc
     2221 gttcgtcctc aaccgccgca ccgcgcagga gatcgggctc gcggttccgc cggcgatgct
     2281 cgtgcgcttc gacagggtga tcgaatgagg tggttactac ttctttgcgt ggtgcccgcg
     2341 ctcgcggccg cggacgaggg cctcgagtcc acggtaaggc aataccgcga ggcgctgcgg
     2401 gacgcctcgc cgggcgagct ctgggtcgtc acgggcgagg agcttttccg cacgccgcgc
     2461 gggccgaaga acgcgtcgct ggaggcctgc gacttcgggc tcgggccggg ggtggtgaaa
     2521 ggcgcctacg cgcagctgcc gcgctatttc gccgacaccg accgggtcga ggacctcgag
     2581 tcgcgcctga tgagctgcat gcggcagttg cagggtttcg gcgaggaggc gttcccgcgg
     2641 catgcgcgcg gcgatcggcg catgctcgat ttcgaacggc tcgcggcgta tgtcgcggcg
     2701 cagtcgaacg gcatgccgct cgcggcgccg ctcgcgcacc cgaaggaggt cgacgcgcag
     2761 cgcatcggcg agcggttgtt ctaccggcgc gccggcacgc acgatttctc gtgcgcgacc
     2821 tgccacacgt tgcagggcaa gcgcattcgc ctgcagcggc tcgcgcagct gacggacccg
     2881 aaagaggcgc aaggcgtctt tcccagctgg cccgcctacc gcgttgcggc gggcgaggtg
     2941 cacacgctcc aggactggat cgcgatctgc tactgggcgg cgcgccatcc gcagctgcgc
     3001 tacggctccg aggcgtcggt ggcactgcag gtgttcatgg cgcggtacgc gaacggcggc
     3061 gtgatctccg cgccgggcgt gaagcgatga agcgcctcgc ctttgccgtc ctgctgtcca
     3121 tgggggcgtc gccgttgatc ggccaggacg atctcgccca gcgcgcggag gcggcgttcg
     3181 tttcctcatt cgccggaggc gatcccgcga tgctcgcacg caccggggtg caggacgagg
     3241 tgcagcgcct gtgttcggag catcgcaact ccccgcccgt cgcgctcgcg caagccatcg
     3301 agcgcaccca gcgcgcgctg atccgctacc cggccagcct catcggcgac tggcgcgaag
     3361 gcaagcgcat cgcggaggac ttcaccggct cgcgctacga cgacaaggcc ggcgcgccga
     3421 acggcggcaa ctgctacgcc tgccaccagc tcgcgcccca ggagctcgcg ttcggcaccg
     3481 tcggcccgag cctgctcgcc tacggcaagt cgcgcggctg gggcgtggcg gcccagcagc
     3541 gcgtttacga tcgaatctac aactcgcagg cgttcgtggc ctgctccgcc atgccgcgct
     3601 tcggccacaa cggcgtgctc actcccgagc agatcgcgca cctggtcgcg ctcttgctcg
     3661 acccgacctc ggaagtgaat cgatgacccg gcggcgcgcc ttcctcgctc tggccgccgc
     3721 ctccctgtgc gtgcctgaaa cgctgctcgc gcaggcgaag ccgcggcgta tcggctacct
     3781 ctcgcctcac tccattaccg aaccgccgtc tccggagcgc gcggacttcc tcgccgggct
     3841 gcgcgagctc ggctatgagc ctgggagaaa cctggtcatc gtctatcgca gcgcggacgg
     3901 agacttcgag cgccttcccg agcttgccgc agaactggtg cggcagcgcg tggagctcat
     3961 cgtggtgaac agcggcgaag cagccatcgc ggcaaaggcg gcgaccagca cgctgccctt
     4021 gatcatcagc gcggccggag acccggtgca gaccgggctg atcaagtcct acgctcgtcc
     4081 gggcggcaac atcaccgggc tgtcgttcat cgcgcccgag ctgggcgcaa agcggctggc
     4141 gctcgccaag gagatcctgc cgcaggcgcg gcgggtggcg gtaatctgga acgcgcgcga
     4201 tccggcctca aacaacgaat gggcccaggt gcgggatgcc ggtcccaagc tcggcatcgc
     4261 gaccgatgcg ctgccggtgg gagaaaccga cgacctggcg cgggcgctcg acttgctgcc
     4321 gaagtcggcg tcgcaggcgc tgctggtgat cgtcgatgcg cgcatggtcg gctttcgcaa
     4381 gatcgtcgcc gacgcggcca tccaggcgcg cattgcctgc tttgccggct ggcgcgagta
     4441 cgtcacctca ggggcgctcg catcgtacgc gccggacttc cgcgcgctgt ttcgccgcgc
     4501 cgcctactac gtcgaccgca tcctgcgcgg cgccaagccc gcggacctgc cggtcgagca
     4561 gccgaccaag ttcgagctgg tggtgaacct caggaccgcg aaggcaatcg gcgtcacggt
     4621 gccgaacgcg gtgctgctgc gcgccgacga ggtgatccaa tgaagcgccg cgcattcctg
     4681 ctggcggcgg gagcgctggc ggcgccgttt gccgcccgag cccaatcggc cgggctacct
     4741 cggatcgggg tgctcatcca cagcactaac tacgccccgg tcgtggaagg gctgcgcgcg
     4801 ggactcaaag agctcggagt cgcggagggg aaagacgtcg cgctggaagt cttcgacgcg
     4861 aagggcgagg cgaaggcgat cgaggaagcc gctcgccgct tcgagcgcga gcgggtgcgg
     4921 ctcatctatt gcacgccgac gtcggccgcc accgtggtga agcgggcgac gagcgaggtg
     4981 ccgatcttct tctgcgtggg aaccgacccg atcgcgagcg gcctcgtcga gagcttcgcc
     5041 aagcccggag ggcggctcac aggcgtgcat tacctgacca cggacctgac ggcgaagcgg
     5101 ctcgagctgc tgaaggaact ggtgccgaag ctgcgccgcg tggtgaccct ctaccgcccg
     5161 ggccaccccc ctgccgaggc atcggcgcgg ttggcccggg aggcgggcaa gaagctcggg
     5221 gtccaggtca
//
LOCUS       DASPXW010000153         3329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1310422, whole genome shotgun sequence.
ACCESSION   DASPXW010000153 DASPXW010000000
VERSION     DASPXW010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310422"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1419)
                     /locus_tag="VEL04_03665"
     CDS             complement(<1..1419)
                     /locus_tag="VEL04_03665"
                     /inference="COORDINATES: protein motif:HMM:NF012430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYT47494.1"
                     /translation="MAKQAVYDASAIQALKGLEPVRRMPGMYTHTVHPLHLVQEAIDN
                     AVDEALSGFGKRITVSVRKDGSVEIDDQGRGIPVDLHKEEGKPAVEVAFTMLHAGGKF
                     SRSGDGVYHISGGLHGVGVAVSNALSKRCEVTVFRDAQKHTIVFEGGELKSKLKSEKS
                     RDKRSGTVVRLWPDPKYFDSPNIPMNELEHLVRSKAYLLPGLTMVLEVESKPSKTWRY
                     ERGMAQYFDFMLQGRELIAPLFAGERFFDEKATREIADVEPGEGAAWAIGWVADGEVF
                     ADSHVNLIPTRSGGTHEAGFRSGIFDALAAFMDTRSLAPKGVKLIPDDLWQRACFTLS
                     ARIVRTQFHGQTKEKLTTRHAAKLMEMCVRDAFELWLNEHPDEGKKIVELAIEQALAR
                     LSKGKKVERKKSSGIATLPGKLVDCASGDITRNELFLVEGDSAGGSAKEARDKETQAL
                     LPLRGKVLNTMSKDSRTVLANKE"
     gene            complement(1473..1931)
                     /locus_tag="VEL04_03670"
     CDS             complement(1473..1931)
                     /locus_tag="VEL04_03670"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HYT47495.1"
                     /translation="MAVVFDCSVTLAWFLRDERTAFTDTAFELLETAECWVPFLWRLE
                     FPNALLFAERKKRVGREQRLEMLDNAAQLFLRTDHALPDIRSLSALADRRGLTVYDAS
                     YLELAVRSGFDLITLDRDLADAAAAEGVAVHSPGRSTAAQVRRRYAARVA"
     gene            complement(1931..2188)
                     /locus_tag="VEL04_03675"
     CDS             complement(1931..2188)
                     /locus_tag="VEL04_03675"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HYT47496.1"
                     /translation="MTTVGSFEAKTKLAELLDKVEAGETVTITRRGRAVAKLVPAVAD
                     EMERARRRALIDEIKRKRAGWDRGAKPGSTIPELIKAGRRY"
     gene            2241..2945
                     /locus_tag="VEL04_03680"
     CDS             2241..2945
                     /locus_tag="VEL04_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease activity regulator RraA"
                     /protein_id="HYT47497.1"
                     /translation="MSSDSRAALKKVSAATLTTVLFKRGFRNTFIQGVFLLNKESPRM
                     VGEAFTLRYIPAREDLDQLGAFEGRGHPQREAIEACPPGQVLVMDARRDASAATGGDI
                     LMTRLMVRGVAGVVTDGGLRDSPTIERLAWPAYCGARSAPLNLVRHHAVDSQVPIGCG
                     GVAVYPGDVVVGDGEGVVVIPAKLADEVAKEAEAQTQFEDWVEVKVKEGRSIFGLYPP
                     NAETKAEYEAWKSKNK"
     gene            2991..>3329
                     /locus_tag="VEL04_03685"
     CDS             2991..>3329
                     /locus_tag="VEL04_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYT47498.1"
                     /translation="MLFRLLSAAALCVAFAAPAAAQETMIVYFAKGFYPAEDKALDEV
                     IDKFQKKTGVKVELSRYAPQEMIPKTVAALDSGTPPDVVYGDVFDFQVAGKWAYEGRL
                     ADLSDILTPMK"
BASE COUNT          560 a         1094 c         1066 g          609 t
ORIGIN      
        1 ctccttgttg gcgagcaccg tgcggctgtc cttcgacatc gtattcagga ccttgccgcg
       61 cagcggcagc agcgcctggg tttccttgtc gcgcgcttct tttgcggagc cgcccgcgga
      121 gtcgccctcc accaggaata gctcattgcg cgtgatatcg ccggaggcgc aatcgaccag
      181 cttgccgggc agcgtggcga tgccgctcga cttcttgcgc tcgaccttct ttcccttgga
      241 caggcgcgcg agcgcctgct cgatcgccag ctcgacgatc ttcttgccct cgtcgggatg
      301 ctcgttcagc cacagctcga aggcgtcgcg cacgcacatt tccatcagct tcgcggcgtg
      361 gcgcgtggtg agcttctcct tcgtctggcc gtggaactgg gtgcgcacga tgcgcgccga
      421 gagcgtgaag caggcgcgct gccacaggtc gtcgggaatc agcttgacgc ccttcggcgc
      481 gagcgagcgc gtgtccatga acgcggcgag cgcgtcgaag atgccggagc ggaagcccgc
      541 ctcgtgcgtg ccgcccgagc gcgtcgggat caggttgacg tggctgtcgg cgaagacctc
      601 gccgtcggcc acccagccga tcgcccatgc agcgccctcg cccggctcta cgtcggcaat
      661 ttctcttgtc gccttttcat cgaaaaacct ttcccccgcg aaaagcggcg cgatcagctc
      721 ccttccctgc agcatgaagt cgaagtactg cgccatgccg cgctcatacc tccaggtttt
      781 agaaggcttg gattcaactt ccaaaaccat cgtcaagccc ggcagcagat acgccttcga
      841 gcgcaccagg tgctccagct cgttcatcgg aatgttgggc gagtcgaagt acttcggatc
      901 cggccacagg cgcaccaccg tgccgctgcg cttgtccctg ctcttctcgc ttttcagctt
      961 gctctttaat tcgccacctt cgaaaacaat cgtgtgcttc tgagcgtccc gaaaaaccgt
     1021 gacctcgcat ctcttcgaca gcgcattcga aaccgcgacg ccgacgccgt gcaggccgcc
     1081 ggagatgtgg tagacgccgt cgcccgagcg cgagaacttg ccgccggcgt gcagcatggt
     1141 gaaggcgact tcgaccgcgg gctttccctc ttccttgtgc aggtcgaccg ggatgccgcg
     1201 gccctggtcg tcgatctcga ccgagccgtc cttgcgcacc gacaccgtga tgcgcttgcc
     1261 gaagcccgag agcgcctcgt cgaccgcgtt gtcgatcgcc tcctgcacca gatgcagcgg
     1321 gtgcacggtg tgggtgtaca tgcccggcat gcggcgcacc ggctccaggc ccttcagcgc
     1381 ctggatcgcc gatgcgtcgt agaccgcctg cttggccatg tatttagttc agtttatcgc
     1441 ttcatccact agatattgta gtccccgccg cctcacgcca cccgcgcagc gtagcgccgg
     1501 cgcacctggg cggccgtcga gcggccgggc gagtggaccg caacgccctc cgcggccgcc
     1561 gcgtccgcga ggtcccggtc gagcgtgatg agatcgaacc cggagcgcac ggcgagctcc
     1621 aggtaagacg cgtcatagac ggtcagcccg cgccggtcgg caagcgcgct cagcgaccgg
     1681 atgtctggca aggcatgatc ggtccgcagg aacagctgag cggcgttgtc gagcatttcc
     1741 agccgctgct cccggccgac gcgctttttt ctctccgcga acagcagcgc attcgggaat
     1801 tcgagccgcc agaggaacgg tacccagcat tcggcggtct ccagcagctc gaacgccgtg
     1861 tcggtgaaag ccgtgcgctc atccctgagg aaccaggcga gcgtcaccga acagtcgaac
     1921 acgaccgcca tcagtagcgt cggccggcct tgatcagctc gggaatggtt gaaccgggct
     1981 tggcgcctct atcccaacca gcgcgcttgc gcttgatttc gtcgatcagc gcgcgccggc
     2041 gagcccgctc catctcgtcg gcgaccgccg gaacgagctt tgcgaccgcc ctgccgcggc
     2101 gggtgatcgt cacggtctcg cccgcctcga ctttatcgag cagctcagct agcttggtct
     2161 tcgcttcaaa cgacccgacc gtggtcacga accctccgtt acaactagac aaccagttgg
     2221 aatcttagca catgacaagc ttgtcttctg acagccgcgc agcactgaag aaagtttcgg
     2281 ccgcgaccct tacgaccgtg ctgttcaagc gcgggttccg caacactttc atccaggggg
     2341 tatttctctt aaataaagaa agtcctagga tggtcggcga agcctttacc cttcgttata
     2401 ttccggcgcg cgaggatctc gaccagctcg gcgcgttcga gggccgcggc cacccgcagc
     2461 gcgaggcgat cgaggcctgc ccgccggggc aggtgctggt gatggacgcg aggcgcgacg
     2521 cctcggcggc gaccggcggc gacatcctga tgacgcgcct gatggtgcgc ggcgtggcgg
     2581 gcgtggtcac cgacggcggg ctgcgcgatt cgccgaccat cgagcggctc gcctggccgg
     2641 catactgcgg cgcgcgctcg gcgccgctca acctggtgcg ccaccacgcg gtcgacagcc
     2701 aggtgccgat cggctgcggc ggcgtcgcgg tctatccggg cgacgtggtg gtcggcgatg
     2761 gcgaaggcgt ggtggtgatc ccggcgaagc tcgccgacga ggtggcgaag gaagccgagg
     2821 cgcagacgca gttcgaggac tgggtggagg tgaaagtgaa ggaagggcgc agcatcttcg
     2881 gcctctatcc gccgaacgcc gagaccaagg ccgagtacga ggcctggaaa tccaagaaca
     2941 aataattgtc ggttatcata caagtcgacc ctagaggagg cgttgatccc atgttgttcc
     3001 gattgctgag tgcggcagcg ctgtgcgttg cattcgcggc tccggccgcg gcgcaggaga
     3061 cgatgatcgt gtacttcgcc aaggggttct acccggcgga ggacaaggcg ctcgacgagg
     3121 tcatcgacaa gttccagaag aagaccggcg tgaaggtgga gctttcgcgc tacgcgccgc
     3181 aggagatgat cccgaagacg gtggccgcgc tcgattcggg cacgccgccc gacgtggtgt
     3241 acggcgacgt gttcgacttc caggtcgcgg gcaagtgggc ctacgagggc cggctcgcgg
     3301 acctgtcgga catcctcacg ccgatgaag
//
LOCUS       DASPXW010000154         2620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1433635, whole genome shotgun sequence.
ACCESSION   DASPXW010000154 DASPXW010000000
VERSION     DASPXW010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1433635"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VEL04_03690"
     CDS             <1..423
                     /locus_tag="VEL04_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47499.1"
                     /translation="EPWPDEDALTALRYKHQAEHGALGMRACKLTEGGYCTLFSEGMA
                     LRGRPGLEGRIAEACSKVYDDEFGHMLAGIAGIAEEGLKDSDWKLLEALAVEQLRQRM
                     RMRNAQFSFPLTEERVQAIWRGEIKPLEFDYRRARLAA"
     gene            complement(420..815)
                     /locus_tag="VEL04_03695"
     CDS             complement(420..815)
                     /locus_tag="VEL04_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47500.1"
                     /translation="MATKPEPTNELKVDPNALFLEEVFTDRRVGTIRRLTPVKKDGTR
                     DTAKAVLYIGETQVLTPAGALPIGFEIGAGSLEEAAEKFGQLAKEAIERTVKELQELR
                     RQAASSIVIPQGGLPPVGGMPGGGKIQMP"
     gene            complement(818..1171)
                     /locus_tag="VEL04_03700"
     CDS             complement(818..1171)
                     /locus_tag="VEL04_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47501.1"
                     /translation="MGLAKLALIFGAALAMAVPPNEAEAGGRGRGGHSGGARHHAHHH
                     HGGAVFFGGYFYTGPYYYYPPYYYPYYEVPLYDQGPENPYWYYCRAANAYYPYVQSCP
                     GGWERYLPGPESPVG"
     gene            complement(1236..1856)
                     /locus_tag="VEL04_03705"
     CDS             complement(1236..1856)
                     /locus_tag="VEL04_03705"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HYT47502.1"
                     /translation="MPRTNLRRYFRHGLFPQLMVFEAAARLGGVTRAAEELHLAQPTV
                     SMQLKKLSETLELALFEQRGRHLHLTSAGEALRELCDELIELFSRAEARLAGLRGPKA
                     ETLRLAAEPEARQVAARLLAAFCSRHPGIHATLHIADRAELLARIAVGVDDVYLFELE
                     VDGLPLERRWSVAHAKGRELARSAAAFVREALLLDAYNSEPAVGDK"
     gene            2088..>2620
                     /locus_tag="VEL04_03710"
     CDS             2088..>2620
                     /locus_tag="VEL04_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HYT47503.1"
                     /translation="MPIPDFLPALAAIVVIDLVLAGDNAIVIALAARNLPRELQRQAI
                     VWGALGAVAVRASLTIAVLWLLQIPGLMLAGGALLIWIAYRLLTGDDAGLARDVAPAV
                     SFWAAMRTIVVADAVMGIDNVLAVAGAARGSMLLVVLGLLISIPIVVWGSTLILRWLE
                     RFPALLYLGGAVLAWTAA"
BASE COUNT          424 a          875 c          881 g          440 t
ORIGIN      
        1 gagccctggc cggatgagga cgccctgacc gcgctgcgct acaagcacca ggccgagcat
       61 ggcgcgctcg gcatgcgcgc gtgcaagttg accgaaggcg gttactgcac gctgttctcg
      121 gaaggcatgg ctctaagggg aaggccggga ctcgaaggca ggatcgccga ggcctgctcc
      181 aaggtctacg acgacgagtt cgggcacatg ctcgccggca ttgccggaat cgccgaggaa
      241 ggcctgaaag acagcgattg gaagctgctc gaggcgctgg ccgtcgagca gctcaggcag
      301 cgcatgcgca tgcgcaacgc gcagttcagc ttcccgctga cggaagagcg cgtgcaggcg
      361 atctggcggg gagaaatcaa gccgctcgag ttcgactacc gccgcgcgcg cctcgcggct
      421 taaggcatct ggatcttgcc gccgccgggc atgccgccga ccggcggcag gccaccctgc
      481 gggatgacga tcgaggaagc cgcctggcgg cgcagctcct gcagctcctt cacggtgcgc
      541 tcgatcgcct ccttggcgag ctggccgaac ttctcggccg cttcctcgag cgagccggcg
      601 ccgatctcga agccgatcgg cagcgcccct gcgggggtga gcacctgcgt ttcgccgatg
      661 tagagaacgg ccttcgccgt atcgcgcgtg ccgtctttct tgaccggcgt cagccggcgg
      721 atcgtgccga cgcgccggtc ggtgaagact tcctcgagaa acagcgcgtt cgggtcgact
      781 ttgagctcgt tggtcggttc gggtttggtg gccatggtca acctaccggc gactcggggc
      841 ccggcagata gcgctcccat ccgccggggc agctctgtac atagggatag taggcattgg
      901 cagcgcggca gtaataccag tacggatttt ccgggccctg atcgtagagc ggcacttcat
      961 agtacgggta gtaatagggc ggatagtagt aatacggtcc ggtataaaag tagccgccaa
     1021 agaacaccgc gccgccgtga tgatgatgag catgatggcg ggcgccgccg gaatgcccgc
     1081 cgcggcctcg tccgccggcc tccgcctcgt tcggcgggac cgccatcgcg agcgcggcgc
     1141 cgaagatcag cgcgagcttc gccaagccca tggcgcgaag gatagcaccg ctacgccacc
     1201 gccatgacga gcggtgtagt tgcgcgggag ctccatcact tgtcccccac ggctggctcc
     1261 gaattgtacg cgtcgagaag cagcgcctcg cgcacgaagg ccgcggccga gcgtgccagc
     1321 tctcgcccct tggcatgcgc cacgctccag cggcgctcaa gcggcaggcc gtcgacttcg
     1381 agctcgaaca ggtagacgtc gtccacgccg acggctatac gcgcgagcag ctcggcgcgg
     1441 tccgcgatat gcagagtcgc atgaattccg gggtgtcgcg agcagaacgc cgcgaggagc
     1501 cgcgccgcca cctgcctcgc ctcgggctcg gcggcgagcc gcagcgtttc cgccttcggc
     1561 ccgcgcagcc ctgcaagacg ggcctcggcg cgcgagaaca gctcgatcag ctcgtcgcac
     1621 agctcgcgta gcgcctcgcc ggcggacgtg aggtgcaggt gccgcccgcg ctgttcgaat
     1681 agcgcgagct cgagggtttc cgagagcttt ttcaactgca tggataccgt gggctgcgcg
     1741 agatggagtt cctcggcggc gcgcgtcacg ccacccagac gcgcagctgc ctcgaacacc
     1801 atcaactggg gaaacaggcc atggcggaag tagcggcgca ggttggtgcg aggcatctcg
     1861 gctccttgtc gaagcccgag gtctcgcttg cagcgctttg gctgccggtg cgaccgggga
     1921 cgaatcccgg catgacgatc gcactgcttt agaagctact cccctctgag ggtgactcga
     1981 tcataactgc cgatcagccc gagatcaatg atccatcgcg attcgcgatg gattgcattg
     2041 cacaaagtca attttcgtgc cgccctgcgc cgctcagaat ctgcgccatg ccgatcccgg
     2101 atttcctccc cgcgctcgcc gcgatcgtgg tcatcgacct ggtgctcgcg ggcgacaacg
     2161 cgatcgtgat cgccctggcg gcacgcaacc tgccccgcga gctgcagcgt caggcgatcg
     2221 tctggggcgc cctcggcgcg gtagccgtgc gcgccagcct cacgatagcc gtgctctggc
     2281 tgctgcagat cccgggactg atgctcgccg gcggcgcgct gctcatctgg atcgcgtatc
     2341 gactcctcac cggagacgac gccgggctcg cgcgcgacgt cgcgcccgcc gtgagcttct
     2401 gggccgcgat gcgcaccatc gtcgtcgccg acgcggtcat gggcatcgac aatgtgctcg
     2461 cagtcgcagg cgccgcccgc ggcagcatgc tgctcgtggt actgggactg ctaatcagca
     2521 ttccgatcgt ggtctggggt tcgacgctga tcctgcgctg gctcgagcgc tttccggccc
     2581 tgctctacct gggcggcgcg gtgctcgcct ggaccgcggc
//
LOCUS       DASPXW010000155         2433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_424505, whole genome shotgun sequence.
ACCESSION   DASPXW010000155 DASPXW010000000
VERSION     DASPXW010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424505"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VEL04_03715"
     CDS             complement(<1..349)
                     /locus_tag="VEL04_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47504.1"
                     /translation="MAGCRLAAVLAALALAGCAGPATSPISVTDRSTFVPSVRVAADI
                     SRYAGEPSDPHGAHALELGLSGARGSGMQMLSAGENPVVFGGQQFDPPQQLRHEFTFR
                     FYELAYRWRQFLGA"
     gene            complement(359..1096)
                     /locus_tag="VEL04_03720"
     CDS             complement(359..1096)
                     /locus_tag="VEL04_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47505.1"
                     /translation="MKVSRLAAVLAVMLIPAGPAFAFDTTEIKANGGSVLPSLGVTID
                     VVGDSALPTATRSSHAAEIGFIAGRSRHKQELDAGDKPIVFGGQTFSGPQDLHYTANI
                     RFADLVYRYRRFFGQSNFAIEGLGGIGWASLGLKAVGPAQSAAVNLSDAGLVLGVGGI
                     WRFQPATSLQLRLTAFGSGSTEGVTSASRFEVYVAHALARNLNVRAGLGSLSARSARE
                     DDRDSNSTNSPIRASASGVLLGLDFVF"
     gene            complement(1131..2246)
                     /locus_tag="VEL04_03725"
     CDS             complement(1131..2246)
                     /locus_tag="VEL04_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYT47506.1"
                     /translation="MKALKKNRGQSPKPNPRSRSARRPLVRGPGPAFQRIAAEEAWAP
                     PEILARYKKLLEEKPASWDPGFRSLWGFFLGQTTRATQLVTRIQDLGERRLADMDASG
                     IARQLVFLTAPGVQVFEASLACSLATEANDQLAEAIRKNPSRYAGLAAIAPQSPREAV
                     KEMERSVKNLGLKGAIINSHTQGEYLDDPKYWEIFEAAQALGVPIYLHPNTPPPDMIQ
                     PFLPRGLDGAIYGFAVETGLHMLRLVLAGVFDRFPRLAMVLGHLGEGLPFWLFRIDFM
                     HRSMVAASRYEGVKALKKKPSEYLKENVWVTTSGMQWAPAIMFTLSVLGTDRVLYAMD
                     YPYQFVPEEVRVTDELPISNADRKKLYQLNAEKVFNL"
BASE COUNT          391 a          795 c          847 g          400 t
ORIGIN      
        1 cggcgcccag gaactggcgc cagcggtagg cgagctcgta gaagcggaaa gtgaactcgt
       61 gcctgagctg ctgcggcggg tcgaactgct ggccgccgaa caccaccggg ttctcgccgg
      121 cagagagcat ctgcatgccg ctgccgcgcg ctccgctcag gccgagctcg agggcgtgcg
      181 cgccgtgcgg gtcggaaggc tcgcccgcgt agcgggaaat atcggccgcg acgcgcaccg
      241 agggcacaaa ggtgctgcgg tcggtgacgc tgatcgggct cgtcgccgga ccggcgcagc
      301 ccgcgagcgc cagcgcggcg aggacggcgg cgaggcggca gccggccacg cgccgccgct
      361 agaagacgaa gtcgagcccg agcaggacgc cgctcgcgct cgcccggatc ggcgaattgg
      421 tcgagttgct gtcgcggtcg tcttcgcgcg ccgagcgcgc gctcaggctg ccgaggccgg
      481 cgcgcacgtt gaggttgcgc gccagggcat gggcgacgta gacctcgaag cgcgaggcgc
      541 tcgtcacgcc ctcggtgctt cccgagccga aggcagtgag gcgcagctgc aggctggtcg
      601 caggctggaa gcgccagatg ccgccgacgc cgaggaccag gccggcgtcg ctcaggttca
      661 cggccgccga ttgcgcggga ccgaccgctt tcaggccgag gctcgcccag ccgatgccgc
      721 cgaggccttc gatggcgaag ttgctctggc cgaagaacct gcggtagcgg tagacgaggt
      781 cggcgaagcg gatgttcgcc gtatagtgca ggtcctgcgg cccgctgaag gtctggccgc
      841 cgaacacgat cggcttgtcg cccgcatcca gctcctgctt gtgcctgctg cggccggcga
      901 taaagccgat ctcggccgcg tgcgagctgc gcgtcgccgt cggcagcgcc gaatcgccga
      961 ccacgtcgat cgtcacgccg agcgacggca ggacggaccc gccgttggcc ttgatctcgg
     1021 tggtgtcgaa cgcgaaggcc gggccggcgg ggatcagcat cacggccagc acggcggcaa
     1081 gacgagaaac cttcatggca gcctcctttc ttgtttgact ccgggatgag ttacagattg
     1141 aacacctttt cggcgttgag ctggtagagc ttcttgcggt cggcgttgct gatcggcaac
     1201 tcgtcggtga cccgcacttc ctccgggacg aactggtagg ggtagtccat ggcatagagt
     1261 acgcggtcgg tcccgaggac cgacagcgtg aacatgatcg ccggcgccca ttgcatgccg
     1321 ctcgtcgtca cccagacgtt ctccttcagg tattcgctgg gtttcttctt caatgctttg
     1381 acgccctcat agcggctcgc cgcaaccatc gagcggtgca tgaagtcgat gcggaacagc
     1441 cagaacggca gcccctcgcc gaggtggccg agcaccatcg ccaggcgcgg gaagcggtcg
     1501 aacacgccgg cgagcaccag ccgcagcatg tgcaggccgg tctcgaccgc gaagccgtag
     1561 atcgcgccgt cgaggccgcg cggcaggaac ggctggatca tgtccggcgg cggggtgttc
     1621 gggtgcaggt agatcggcac cccgagcgcc tgcgccgcct cgaagatctc ccagtacttc
     1681 ggatcgtcga ggtactcgcc ctgggtgtgc gagttgatga tcgcgccttt caggccgagg
     1741 tttttcacgc tccgttccat ttccttgacg gcctcgcgcg ggctctgcgg cgcgatcgcg
     1801 gcgagcccgg cgtagcggga gggatttttc cgaatcgctt ctgccagctg gtcgttcgcc
     1861 tctgtagcca gtgaacaagc aagagaagct tcaaaaacct gcaccccggg cgcggtgagg
     1921 aacacgagct gcctggctat cccgctcgcg tccatgtcgg cgaggcggcg ctcgcccagg
     1981 tcctggatgc gcgtcacgag ctgtgtcgcg cgggtggtct gcccgaggaa gaatccccac
     2041 aggctgcgaa agcccgggtc ccaggaagcc ggtttttcct ccagaagttt tttgtaccgc
     2101 gcaagaatct ccggcggcgc ccaggcctcc tcggcggcga tgcgctgaaa ggccggcccc
     2161 ggccccctga caagggggcg gcgagcggaa cgcgagcggg ggtttggttt cgggctctga
     2221 ccccgatttt tcttcagggc tttcatgccc caagtctagc taaactgcga ggcgtaatgc
     2281 tctccaaagc ggaactgttc gcgcgcctcg ccgaaggcca cgccgcgcgc atcacggtgg
     2341 tgacgccgaa caagcgcctc tcccaggcgc tgatgtccga gttcgacgct ttccagatcg
     2401 cgaagcgcct gacggtctgg gaggcgcccg aca
//
LOCUS       DASPXW010000156         3571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_801914, whole genome shotgun sequence.
ACCESSION   DASPXW010000156 DASPXW010000000
VERSION     DASPXW010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801914"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(306..956)
                     /locus_tag="VEL04_03730"
     CDS             complement(306..956)
                     /locus_tag="VEL04_03730"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYT47507.1"
                     /translation="MIRVLICDDQQVVCEGLRAILGTAPQIEVVGIAGDGAQAVARVA
                     TTRPDVVLMDLKMPVLNGVQATREIRTRFPGVRVLVLTTYDADQWVLNAIRAGASGYL
                     LKDCPGDTLIAAVEDTAAGKTHLDPAVAGRLLDQLSRGAARAPRPLPGSLTERERQVL
                     GLLGRGWGNAAIAAHLHMAEGTVRNHVSALLGKLGVADRTQAALLALRCGLVEPDA"
     gene            complement(953..2197)
                     /locus_tag="VEL04_03735"
     CDS             complement(953..2197)
                     /locus_tag="VEL04_03735"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HYT47508.1"
                     /translation="MNHPGRRLVEPGILPLFRIFVALEILLLLLRIGLEALFRSDFPL
                     VQPAWPGLVFLALLLGYLSWRRLELALGRFYLPVALALSIGVTIVSAAAGLKLRLDAG
                     IRAEELVRGSWILIVVLLVPLIIVAWQYGFRWVLWFCALTVATELALTLPLAGRGGPP
                     APTLAVIALVRCLFFLPVGYAVARLARAQREQRAALAEANARLARYASTMEQLAVSRE
                     RNRLAHELHDTLAHGLSSVAVQLEAMRALWKSPETARAMLTDALDATRTALGEARRAI
                     AALRASPLEDLGLARALRALAESQAARAGLALELDVSGSLEGLDSEVEHAVYRIAAEA
                     LANAVRHAQARRLTVRLEHAGSRLRLLLADDGRGFDPAAAAGDGRFGLEGMRERARII
                     DATLEIESAAGKGTTVRLTVER"
     gene            complement(2229..3425)
                     /locus_tag="VEL04_03740"
     CDS             complement(2229..3425)
                     /locus_tag="VEL04_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47509.1"
                     /translation="MLALLRPPAWPGIRDAAASERESARRGVPRRFQTVYNELARLLD
                     DFERFLRSRPARRRDVVFGGEVLAANAHRGEDLLTEQGYKGSLVYIDRLKGLGAGGVT
                     VQAAYPLLADDFPRAAEYWAFYRRLAQAVKERGLKLHVKNGPLFTEKEFSKLKVRPDY
                     SRLTVERYFEARMRIAQRAAEQLAPDYLTIGNEPSSEMHILKFGITAERYTRFVNETI
                     QGMKRTGTLFGAGSGNWDSLDYIRRFSNETALDYIDLHIYPLASPHENFLQRAVEMAG
                     IARAARKRLVVGEAWLYKAAPRELGANPTAASVFARDIYSFWAPLDIRFLEVLHRFGQ
                     TQQAEYVSPFWTKYLFGYVDFDRAPALASAQQLLQLGDQAAVKQIVAGKVSDSGGAYR
                     RIARAD"
BASE COUNT          583 a         1207 c         1251 g          530 t
ORIGIN      
        1 gggcgaagtc ttcgcccctc acactcagcg caggctggcg gcgcggagcc ggggaggtta
       61 tttaccacta acccgcccga tcgcacccct ccggagggtg cggaggtgag cgaaattgac
      121 ataagacaga gtgcgtggtt ccagacagga ccagcaccaa attgcggcgc gccggcgggc
      181 ccttcagact tcgcggccgg attgattcgc ctatcccttg gatggacggg cgccggggtt
      241 tttgttggcg gcggcgatat tgagctttcg tctaacgatt tccgcgctcg cgaagtcgac
      301 cgcagctagg cgtcgggttc caccagaccg cagcgaagag caagaagcgc cgcctgcgtg
      361 cgatcggcga cgccgagctt gccgagcagc gcgctcacgt ggttgcgcac ggtgccttcg
      421 gccatgtgca gatgcgccgc gatcgcggcg tttccccagc cgcggccgag cagcccgagc
      481 acctggcgct cgcgctcggt gagggatccg gggagcgggc gcggcgccct cgcggcgccg
      541 cgcgagagct ggtcgagcag gcggccggcg accgcaggat cgagatgggt tttgccggcc
      601 gcggtgtctt ccaccgcggc gatgagcgtg tcgcccggac aatccttgag cagatagccg
      661 gatgcgccgg cgcggatggc attgagcacc cactggtcgg cgtcataggt ggtcaggacg
      721 agcacgcgca cgccaggaaa gcgcgtgcgg atctcgcgcg tcgcctgcac gccgttcagc
      781 accggcatct tgagatccat cagcaccacg tcgggccgcg tggttgccac ccgggcgacg
      841 gcctgcgcgc cgtcgccggc gatgccgacg acttcgattt gcggcgcggt gcccaggatc
      901 gcgcgcaagc cttcgcagac gacctgctga tcgtcgcaga tgaggacgcg tatcaccgtt
      961 ccaccgtgag ccgcaccgta gttccctttc ccgcagcgct ttcgatctcg agcgtggcgt
     1021 caatgatacg cgcgcgttcc cgcatgccct ccagcccgaa gcgtccatcg ccggctgccg
     1081 ccgcagggtc gaagccgcga ccgtcgtcgg cgagcagcag gcgcaggcgc gagcccgcgt
     1141 gctccagcct taccgtgagg cggcgcgcct gcgcatgccg taccgcgttg gcgagcgctt
     1201 cggcggcgat tcggtacacg gcgtgttcca cctcgctgtc gagtccctcg agcgagcccg
     1261 acacgtcgag ctcgagcgcg agcccggcgc gtgcggcctg cgactccgcc agcgcgcgca
     1321 gcgcgcgcgc gaggccgagg tcctcgagcg ggctggcgcg cagcgcggcg atggcacgcc
     1381 gcgcttcgcc cagcgcggtg cgagtcgcgt cgagagcgtc ggtgagcatg gcgcgcgccg
     1441 tctcgggcga cttccacagc gcgcgcatcg cttcgagctg caccgctacc gagctcagtc
     1501 catgcgccag cgtgtcgtgc agctcgtggg cgaggcgatt gcgctcgcgg ctcacggcga
     1561 gctgctccat cgtgctcgcg tagcgggcaa gccgtgcatt cgcctcggcc agggcggctc
     1621 gctgctcacg ctgcgcgcgc gcaagccgcg ccactgcgta gcccaccgga agaaaaaaca
     1681 agcagcgcac cagggcgatg accgccagcg tcggcgcggg cggaccgcct ctgcccgcga
     1741 gcggcaaggt gagcgcgagc tcggtcgcca cggtcagagc gcagaaccac agcacccagc
     1801 gaaagccgta ttgccaggcg acgatgatga gcggcaccag cagcacgacg atcaggatcc
     1861 acgagccacg caccagttcc tcggcccgga tgccggcgtc cagacgcagc ttcaggccgg
     1921 ccgccgcgct gacgatggtg acgccgatgg aaagcgccag cgcgacgggc aggtagaacc
     1981 gccccagcgc cagctcgagg cgccgccacg acaggtagcc cagcagcagc gccaggaaca
     2041 cgaggcctgg ccatgccggc tgcaccagcg ggaagtccga gcgaaatagc gcctcgagcc
     2101 cgatcctgag cagaagcagc aggatctcca gggcgacgaa gatccggaaa agcggcagta
     2161 tgcccggctc gacgagccgc cgcccagggt gattcatgac cgatggtgtg ccgtgcgccg
     2221 ccgaggcgtc aatcggcgcg ggcgatgcgc cggtaggcgc ctcccgaatc ggaaaccttg
     2281 cctgcgacga tctgcttgac cgcggcctgg tcgccgagct gcaggagctg ctgcgcgctg
     2341 gcgagcgccg gcgcgcggtc gaaatccacg tagccgaaga gatacttggt ccagaacggg
     2401 gagacgtatt ccgcctgctg cgtctgcccg aagcggtgca agacctcgag gaaacggatg
     2461 tcgagcggcg cccagaagct gtagatgtcg cgcgcgaaca cgctcgccgc ggtcgggttg
     2521 gctccgagct cgcgcggcgc ggccttgtag agccaggctt ctccgaccac gagacgcttg
     2581 cgcgcggcgc gcgcgatgcc cgccatctcg acggcgcgct gcaggaagtt ctcgtgaggg
     2641 ctggccagcg gatagatgtg caggtcgatg tagtcgagcg cggtctcgtt ggagaacctg
     2701 cggatgtagt cgagcgaatc ccagttgccg ctgcccgcgc cgaacagcgt gcccgtgcgc
     2761 ttcatgccct ggatcgtttc gttcacgaag cgcgtgtagc gctcggcggt gatcccgaac
     2821 ttgaggatgt gcatctcgga ggagggttcg ttgccgatcg tgaggtaatc gggcgcgagc
     2881 tgctcggcgg cccgttgagc gatgcgcatg cgcgcctcga aatagcgctc gaccgtgagc
     2941 ctcgagtagt cgggccggac cttgagcttg gaaaactcct tctcggtgaa aagcgggccg
     3001 ttcttgacat ggagcttcaa gccccgctcc ttgacggcct gcgcaaggcg ccggtagaag
     3061 gcccagtact cggctgcgcg cggaaagtcg tcggccagca gcggatacgc cgcctggacc
     3121 gtgacgccgc ccgcgcccag ccccttgagg cggtcgatat agacgaggct gcccttgtag
     3181 ccctgctccg tcagcaggtc ttcgccacgg tgggcgttgg ccgcaagcac ctcgccgccg
     3241 aacacgacgt cgcgccggcg ggcaggccgg gagcgcagga agcgctcgaa atcgtcgagc
     3301 aaccttgcga gctcgttgta gacggtttga aagcggcggg gaacgccgcg gcgagcggat
     3361 tcgcgctcac tcgcggcggc atcccgaatt cccggccagg ctggcggacg caggagcgcc
     3421 agcatcgcac ccgctaccat ccaccggcgc tccaagttca agtctggtct cccgtcggtg
     3481 aagcgcagtg ccgcgccgca gcgtcgttgc tcccgttatc gcacgacgag cttcttcacc
     3541 gcggggaaca gccacgtcgg gtgcggcgta a
//
LOCUS       DASPXW010000157         2411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_802047, whole genome shotgun sequence.
ACCESSION   DASPXW010000157 DASPXW010000000
VERSION     DASPXW010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802047"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VEL04_03745"
     CDS             <1..622
                     /locus_tag="VEL04_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYT47510.1"
                     /translation="TVFQLLLRYYDPDSGAITLDGVEVRNADPRAVRRRMALVPQDPV
                     IFAASVAENVRYGRPEASDAEVLAACKAAYASEFIERLPEGMNSFLGERGVRLSGGQR
                     QRLSIARALLADRAVLLLDEATSSLDAESEHYVQMALERLMQGRTTLVVAHRLATVKN
                     ADRIVVMDQGRVIATGTHAELVRGEGLYARLAALQFLGEISPEMAQ"
     gene            619..2109
                     /locus_tag="VEL04_03750"
     CDS             619..2109
                     /locus_tag="VEL04_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HYT47511.1"
                     /translation="MRSWVSLFALLLALPVQADTLRIGSKRFTESYILGEVLRRQVEG
                     ITAVEHKPGLGSTGIVVAALKAGSIDLYPEYTGTIAKEFLKLEGHPGIEELNRALNSQ
                     GLGVAVRLGFNNSYALAMREERAQALGIRSLSDLAKHAGLKLGLSQEFIGRADGWPGL
                     KAAYGLPYATPSGLDHGLAYEAIAAGKIDVMDIYSTDAKIERYKLRVLGDDRGFFPRY
                     DAVLLYRLEVPRRFPEAWRALEKLEGRIDELRMIRMNAAAELEGKSFAEAAAHLDSEG
                     KPQSAVRRNFLGALFGPDFWRLTDEHLLLVFVSLAASIAVGIPLGVAAAKVPRAEQAI
                     LGVVGVIQTIPSLALFAFLIALVGTIGTWPALIALFLYALLPIVRNTCAGLIGTSRGM
                     RQAAMALGLSGRDRLWRIEIPLALPSILAGIKTSAVINVGTATIAAFIGAGGYGERIA
                     SGLALNDNVTLLAGAIPAAALALLIQGAFEIGERQFGWMARAQGRS"
BASE COUNT          389 a          793 c          814 g          415 t
ORIGIN      
        1 cactgtgttt cagctcttgc tgcgctatta cgatcccgac agcggagcga tcacgctcga
       61 cggcgtggag gtcaggaacg ccgatccgcg ggcggtgcgg cggaggatgg ccctcgtgcc
      121 gcaggacccg gtcatcttcg cggcgagcgt cgccgagaac gtgcgctacg gccggccgga
      181 ggcgagcgac gcggaagtgc tcgccgcgtg caaggcggcc tacgcgagcg agttcatcga
      241 gcgcctgccc gagggaatga attcgtttct cggcgaacgc ggcgtgcgtc tctcgggcgg
      301 gcagcgccag cgcctttcga tcgcccgcgc tctcctcgcc gaccgcgcgg tgctgctctt
      361 ggacgaggcg acgagctcgc tcgacgccga aagcgagcat tacgtgcaga tggcgctcga
      421 gcgcctgatg cagggccgca ccacgctcgt cgtcgcccac cggctcgcca cggtgaagaa
      481 cgccgaccgc atcgtcgtga tggaccaggg ccgggtgatc gccactggca cgcacgcgga
      541 gctggtgcgc ggggaagggc tctatgcgcg gctcgccgcg ctgcagttcc tcggcgagat
      601 ctcgcccgag atggcgcaat gaggagctgg gtgtcgctct tcgcgttgct attggcgctc
      661 cccgtgcagg ccgacaccct gcgcatcggc tccaagcgct tcaccgagtc ctatattctc
      721 ggcgaggtgc tgaggcggca ggtcgagggg atcacggcgg tcgagcacaa gccgggactc
      781 gggagcacgg gcatcgtcgt cgccgcgctg aaggccggct cgatcgacct ctatcccgag
      841 tacaccggaa cgatcgccaa ggaattcctc aagctcgaag gccatcccgg gatcgaggag
      901 ctgaaccgcg cgctcaattc ccagggtctc ggggtcgcgg tgcggctcgg cttcaacaac
      961 agctacgcgc tcgcgatgcg cgaggagcgg gcgcaggcgc tcggtatccg ctcgctctcg
     1021 gaccttgcga agcacgccgg gctcaagctc gggctttcgc aggaattcat cggccgcgcc
     1081 gacggctggc cggggttgaa ggcagcctac ggcttgccgt acgccacgcc atcgggtcta
     1141 gatcacggcc ttgcctacga ggcgatcgcg gcggggaaga tcgacgtgat ggacatctac
     1201 tcgaccgacg ccaagatcga gcggtacaaa ctccgcgtgc tcggggacga cagaggcttc
     1261 tttccgcgct acgacgcggt gctgctctac cggctggagg tgccgcggcg ctttcccgaa
     1321 gcgtggcgcg cgctcgagaa gctcgaaggc cgcatcgacg agctccgcat gatccgcatg
     1381 aacgccgcgg cggagctcga agggaagagc ttcgccgaag cggcggcgca cctcgactcg
     1441 gagggcaagc cacagagtgc ggtgaggaga aattttctgg gcgcattgtt cggtccggat
     1501 ttctggcggt tgacggacga gcacctgctg ctggtgttcg tctcgctcgc ggcgagcatc
     1561 gccgtcggca ttccgctcgg cgtcgctgcc gcgaaagtgc cgagggccga gcaagccatc
     1621 ctcggcgtcg tgggcgtgat ccagacgatc ccgtcgctcg cgctcttcgc atttctcatc
     1681 gcgctcgtcg gcacgatcgg cacctggccc gcgctgatcg ctctgtttct ctacgcgctg
     1741 ctgccgatcg tgcgcaacac ctgcgcgggg ctcatcggga ccagccgcgg catgcgccag
     1801 gcggcgatgg cgctcgggct ttccgggcgc gaccggctgt ggcgcatcga aatcccactc
     1861 gccttgcctt cgatcctcgc cggcatcaag acttccgcgg tgatcaacgt cggcaccgcg
     1921 accatcgcgg ccttcatcgg cgcgggcggc tacggcgagc gcatcgcttc gggcctcgcg
     1981 ttgaacgaca acgtgaccct gctcgcgggc gccataccgg cggccgcgct cgccctgctg
     2041 atccagggcg cgttcgagat cggcgaacgg caattcggct ggatggcgcg ggcgcagggg
     2101 cgctcgtagc ggctcttaat tgcagcggcg acgccgcacc ccgtcgagca gccgcggacc
     2161 ggggccgctt cccgtcatcc cacgtcccta gaatcccctt gattcaccgg ccattaagta
     2221 cgtccgcgcc gcctatcccg tattgattgg cacgaaaccg caccgcagca acatgactat
     2281 acgtacatac agtaccgtaa cgcgttgatt tttagaaggg atggcaagga cgggcgttcg
     2341 ggtgtctaat ataggggaca tttcggaagt tcactatacg ttgaactaaa ccgcttacgc
     2401 ggtggtggtc t
//
LOCUS       DASPXW010000158         5701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1811766, whole genome shotgun sequence.
ACCESSION   DASPXW010000158 DASPXW010000000
VERSION     DASPXW010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5701
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1811766"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="VEL04_03755"
     CDS             <1..800
                     /locus_tag="VEL04_03755"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019248911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HYT47512.1"
                     /translation="LLARMMDDNARLSEPLDSIEVMREAVIRSALESARQAEAWGLGA
                     ERIVISCKMSQVQDLIAVYRALGERCAYALHLGLTEAGMGSKGIVASTAAMAVLLQEG
                     IGDTIRVSLTPEPGGERTREVQVAQEILQTMGLRAFTPMVSACPGCGRTTSTVFQELA
                     DRIQKFLRTQMPIWRESYPGVEEMRVAVMGSVVNGPGESKHADIGISLPGTNEVPVAP
                     VYVDGKKTVTLKGERIAEEFQAIVENYVKQRFGSGQTTRRSIKLKTL"
     gene            805..2064
                     /gene="hisS"
                     /locus_tag="VEL04_03760"
     CDS             805..2064
                     /gene="hisS"
                     /locus_tag="VEL04_03760"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HYT47513.1"
                     /translation="MSTIQAVRGMNDVLPDEAALWREFESKVEAWLERYGYRNMRMPL
                     VEHTQLFRRAIGEATDIVEKEMYSWRDELNSEELTLRPEGTASCVRAAIEHSLLHDGP
                     KRLWYSGPMYRHERPQKGRYRQFHQYGVEAIGFAGPDVDAEHIAMCSALWRILQLRNI
                     RLELNNIGSPQERVRYRADLVRYLEKHQDRLDADAKRRMHSNPLRTLDSKNPIVQELV
                     ASAPPLASYLGDASLAHFEGLQRLLKAAGIDFTLNPGLVRGLDYYNLTVFEWAAADLG
                     AQRTVCGGGRYDGLFAQLGGKPAPATGFAMGNERLLALMREAGRNPQPRPPHGYVVFQ
                     GEGTHEYAFAMAEELRSKDFSIMLHAGGGSLKSQMKRADASGALYALIVGEDELKAGE
                     VSVKPLRIEREQLRVRRERVAELLKGS"
     gene            2064..2696
                     /locus_tag="VEL04_03765"
     CDS             2064..2696
                     /locus_tag="VEL04_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYT47514.1"
                     /translation="MALDLEEQEQVAELRAWWQQHGGLVVAVVVAAALAFAGWQGWRI
                     YQQSQATQAGALFDTLARAATTGDAKALRDAGGTLIESYPRTLYASMGGLIAARFHFD
                     RNDLKSAKAQLQWVLDRSASAELRDLARLRLAAVLLDEKAYDEALKLLEQKHAPAFEA
                     QYAALKGDVLVAKNQAAGAREAYRLALDKAGKQDAAFRESVRMRLEALGG"
     gene            2699..3859
                     /gene="bamB"
                     /locus_tag="VEL04_03770"
     CDS             2699..3859
                     /gene="bamB"
                     /locus_tag="VEL04_03770"
                     /inference="COORDINATES: protein motif:HMM:TIGR03300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HYT47515.1"
                     /translation="MARFAAALAAAMLAGCSGSTGPMPAELTPLENPRETQVLWYASL
                     TEGDRYSASAFTAPPADAERFVFFPAQVGDAIYAASRQGVLVRLEAAKGKERWRASAA
                     TQLSGGVGADAKTVAVASEKGEVVAFDTERGAVRWRARVSSEVIAPPTVGGGLVLVRS
                     IDNRIFAFGADDGKRRWVYQRAPASLIVRTPSGISLLGDTAFAGFSGGKLVAIALSNG
                     GQRWEATVAIPKGSTELERVTDVIGYPAVQGREVCAVAFQGRVACYEAASGRQVWARD
                     LSSATGVSLDARYAFVSDDRGAVQALDRSNGRSVWKQEKLAHRQLSLPLPSNNAIVVG
                     DLEGFVHFLARDSGAFVARYETRGGAVRAAPMALPSGLLVQTENGTLYALAL"
     gene            3856..5175
                     /gene="der"
                     /locus_tag="VEL04_03775"
                     /pseudo
     CDS             3856..5175
                     /gene="der"
                     /locus_tag="VEL04_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298243.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
     gene            5345..>5701
                     /locus_tag="VEL04_03780"
     CDS             5345..>5701
                     /locus_tag="VEL04_03780"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYT47516.1"
                     /translation="MALVRAGSKLLEKRGTAGLSLREAARKAGVSHNAPYRHFPDRES
                     LLAAIAAEGFAILGEHLKGQAGREKAAAYVRFAIENPQRFRLMSRGGVAATKAYEELV
                     DALSEQADPKQARVVAA"
BASE COUNT          982 a         1842 c         2038 g          839 t
ORIGIN      
        1 aactgctggc gcgcatgatg gacgataacg cgcgactttc tgaaccattg gattcaattg
       61 aagtaatgcg agaagcagtc atccgctcgg cgctggaatc ggcgcgccag gccgaggcat
      121 ggggccttgg cgccgagcgc atcgtcatct cctgcaagat gagccaggtc caggacctga
      181 tcgcggtgta ccgcgcgctc ggtgagcgct gcgcgtacgc gctgcatctc gggctgaccg
      241 aggccggcat gggatcgaag ggcatcgtcg cctcgaccgc ggcgatggcg gtgctgctgc
      301 aggaaggcat tggcgacacg atccgggtct cgctcacccc cgagccgggg ggcgagcgca
      361 cgcgcgaagt ccaggtggcg caggagatcc tgcagaccat ggggttgcgc gccttcacgc
      421 cgatggtcag cgcctgcccc ggctgcgggc ggaccaccag cacggtgttt caggaactcg
      481 ccgaccgcat ccagaaattc ttgagaactc aaatgcccat ctggcgggag tcctatcccg
      541 gggtggagga aatgcgcgtt gcggtgatgg gctccgtggt gaacgggccg ggcgagtcca
      601 agcatgcgga catcggcatc agcctgccgg gaaccaacga agtcccggtg gcgccggtgt
      661 acgtcgatgg caaaaagacc gtcacgctga aaggcgagcg catcgccgag gagttccagg
      721 ccatcgtcga gaactacgtg aagcaaagat tcggcagtgg gcagaccacg cgtcgcagca
      781 tcaagctgaa aacgctctag gaaaatgtcg accatccagg cggtgcgcgg catgaacgac
      841 gtgctgccag acgaggccgc gctttggcgc gagttcgagt cgaaggtcga ggcctggctc
      901 gagcgctacg gctaccgcaa catgcgcatg ccgctggtcg agcacacgca gctcttccgc
      961 cgtgcgatcg gcgaggcgac cgacatcgtc gagaaggaga tgtattcgtg gcgcgacgag
     1021 ctgaacagcg aggagctcac gctgcgcccc gagggcacgg cgtcgtgcgt gcgcgctgcc
     1081 atcgagcaca gcctgcttca cgacgggccg aagcgcttgt ggtacagcgg gccgatgtac
     1141 cggcacgagc ggccgcagaa gggccgctac cgccagtttc accagtacgg tgtcgaagcg
     1201 atcggctttg ccgggcccga cgtggatgcg gagcacattg ccatgtgctc cgccctatgg
     1261 cggatcctgc agctgcgcaa catcagattg gagctgaaca acatcggctc gccgcaggag
     1321 cgcgtgcgct atcgcgccga cctggtgcgc tacctggaaa agcaccagga ccggctcgat
     1381 gccgacgcga agcggcgcat gcacagcaat ccgctgcgca ctctcgactc gaagaacccg
     1441 atagtccagg agctcgtcgc ctcggcgccg ccgctcgcga gctacctggg cgacgcctcg
     1501 ctcgctcatt tcgaggggct gcagcgcctg ctgaaggccg cggggatcga tttcacgctg
     1561 aacccggggc tggtgcgcgg gctcgattac tacaatctga cggtgttcga gtgggccgcg
     1621 gcggatctcg gcgcgcagcg cacggtctgc ggcggcggcc gctacgatgg gctgttcgcg
     1681 cagctcggcg gcaagccggc gcccgcgacc ggcttcgcga tgggcaacga gcggctgctc
     1741 gcgctgatgc gcgaggccgg ccgcaacccg cagccgcggc cgccgcatgg ctacgtggtg
     1801 ttccagggcg agggcaccca cgagtacgcc ttcgccatgg cggaggaact gcgttcgaaa
     1861 gacttcagca tcatgctgca cgccggcggg ggcagcctga agtcgcagat gaagagggcc
     1921 gacgcgagcg gtgcgctcta cgcgctgatc gtgggcgagg atgaactcaa ggcgggcgag
     1981 gtgtcggtga agcccctgag aatcgaacgc gagcagctgc gggtgcggcg cgaacgcgtc
     2041 gccgaactgc tgaaaggaag ctgatggcgc tggatctgga agagcaggag caggtcgccg
     2101 agctgagggc ctggtggcag caacacggcg ggctggtggt cgccgtggtg gtcgcggccg
     2161 cgctcgcctt cgcggggtgg cagggatggc gcatctacca gcagagccag gcgacgcagg
     2221 ccggggcgct gttcgatacg ctcgcgcggg cggcgacgac cggggatgcg aaggcactgc
     2281 gcgatgccgg cggcacgctg atcgagagct atccccgcac cctgtacgcg tcgatgggcg
     2341 ggctgatcgc ggcgcgcttc cattttgacc gcaacgacct gaagagcgcc aaggcgcagc
     2401 tgcagtgggt gctcgaccgg tcggcttcgg ctgagctgcg cgatctggcc cgcctgcgcc
     2461 tcgccgccgt gctgctggac gaaaaggcct acgacgaggc gctcaagctg ctcgagcaga
     2521 agcacgcgcc ggcgttcgaa gcgcagtacg ccgcactgaa gggcgatgtg ctggtggcga
     2581 agaaccaggc ggccggcgcg cgcgaggcct accggctcgc tctcgacaag gccggcaagc
     2641 aggacgcggc gttccgcgaa agcgtgcgca tgcggctcga ggcgctcgga ggctgagcat
     2701 ggcgcgtttc gccgcagcgc tcgccgctgc gatgctcgcg ggatgcagcg ggagcaccgg
     2761 gccgatgccg gccgagttga cgccgctcga gaatccgcgg gaaacgcagg tcctgtggta
     2821 tgcgtcgctc accgaaggcg atcgctacag cgcatccgcc ttcactgcgc cgcccgccga
     2881 cgccgaacgc ttcgtgttct ttcccgccca ggtcggcgat gcgatctacg cagcctcgcg
     2941 gcagggcgtg ctggtgcggc tcgaagccgc gaagggcaag gagcgctggc gcgcatccgc
     3001 cgccacgcag ctctcgggcg gggtcggcgc cgacgcgaaa acggttgcgg tcgcgagcga
     3061 aaagggcgag gtcgtggctt tcgataccga gcgcggcgcg gtgcgctggc gcgcgcgcgt
     3121 ctcgagcgag gtgatcgcgc cgcccaccgt cggcggcggg ctggtgctag tgcgcagcat
     3181 cgacaaccgc atcttcgcgt tcggcgccga tgacggcaag cgccgctggg tctaccagcg
     3241 cgcgccggct tcgcttatcg tgcgcacgcc ttccggcatc tcgctcctcg gcgacaccgc
     3301 gtttgccggc ttctcgggcg ggaagctggt ggcgatcgcg ctctccaacg gcggccagcg
     3361 ctgggaagcc accgtggcga ttccgaaggg ctccaccgag ctcgagcgcg ttaccgatgt
     3421 gatcggctat ccggcggtgc aggggcgcga agtctgcgcc gtcgccttcc aggggagagt
     3481 ggcgtgttac gaggccgcca gcggcaggca ggtctgggcg cgcgacctct cctcggccac
     3541 cggcgtcagt ctcgatgcgc gctacgcctt cgtaagcgac gatcgcggcg ccgtgcaggc
     3601 gctcgatcgc agcaacggac gctcggtgtg gaagcaggaa aagctggcgc accgccagct
     3661 gtcgctgccg ctgccgagca acaatgcgat cgtggtcggc gacctggaag gtttcgtgca
     3721 tttcctcgcg cgcgactcgg gtgccttcgt ggcgcgctac gagacgcgcg gcggcgcggt
     3781 gcgcgccgcc ccgatggcgc tgccgtccgg actgctggtg caaacggaga acggcaccct
     3841 gtatgcgctg gcgctgtgaa accggtgatc gcgctggtcg ggcgcccgaa cgtcggcaag
     3901 tcgacccttt ttaaccgcct cacgcgcagc cgcgacgcga tcgtgcacga cctgcccggc
     3961 gtgacgcgcg atcgccatta cggcgaaggc cgcctcggcg cacgagcttt catcgccatc
     4021 gacaccggcg gcttcgagcc ggccgccgcc gagggcatct tcgtcgagat ggcgcgccag
     4081 gccgagcagg caattgccga ggccgacgcg gtgatcctgc tggtcgacgg ccgcgccggc
     4141 ctcacgccgg ccgatcggct gatcgccgag aaactgcggc gaaccaggtc gtccgtctac
     4201 atcgcggtca acaagaccga gggcatgcag cccgataccg cgaccgccga attccacgag
     4261 ctcgcgctgg gcacgcccgc cgcgatctcc ggggctcatg gcgagggcgt gcgcgaattg
     4321 atggacttgg ttctcgagga acttcccgaa ggcgatgaaa gcgcggacaa ggacaaaggc
     4381 catccgcgcg tcgcgatcgt tgggcggccg aacgtcggca agtcgactct ggtgaacgca
     4441 ctggtcggcg aagagcgcgt gatcgccttc gatcagccgg gcacgacgcg cgacccgatc
     4501 gaggtgccgt tcgagcgtgg cggccggcgc tacaccttga tcgacaccgc gggcttgcgc
     4561 cgccgcggca agaccggcac gccggtcgag ttcttctcga tcgtgaaggc gctgcaggcg
     4621 atcgaggccg ctaacgtcgc cgtgctgcta ctcgacgcag ccggggaggg cgtcaccgag
     4681 caagacgcac atgtcgcggg ctatatcctc gagcgcggac gcgccgtggt ggtcgcggtg
     4741 aacaagtggg acgccgtcgg caaggaggcg cgcgaccgca tcaaggccga gctgcagtgg
     4801 aagctgggct tcctcggctt cgccgcgcaa cacaccattt cggcaaagca gggcaaaggc
     4861 ctggcccccc tgatgggcgc tgtcgacgcc gcctatgccg cggcgatggc gcgcctgcct
     4921 acgcccaagc tgacccgcgc cctggccgcg gcggtcgaaa agcaggcgcc gccgaaagtc
     4981 ggcatgttcc gccccaagct gcgttacgcg caccagggcg gcgtcaaccc gccgcgcatc
     5041 atcatccatg gcaacgcgct cgagcgcatt gccgagagct acaagcgcta cctggaaagc
     5101 ttcttccgca aggaattcag cctcaccggt acgccgatgg cgatcgagtt ccgcaccggc
     5161 aggaacccgt acgccaccag gggcaagcgg ctgccggcgg gcaagccgcg cgcgcaacgc
     5221 gaagctagga accgatgctg gccgcgggcc aggaagaaaa agcggccctg agctctttgt
     5281 gatatgttgt caatgaccac ctaaaatgta gacagtgaca acataccacc acggcgacct
     5341 gcgggtggcg ctggtccgcg caggatccaa gttgttggaa aagagaggga ctgcgggcct
     5401 cagcctgcgt gaagccgcac gcaaagcggg ggtttcgcac aatgctccct atcgccactt
     5461 ccccgaccgt gaaagtctgc tggcggcgat cgccgcggag ggtttcgcga tactgggcga
     5521 gcacctgaag gggcaggccg gccgcgagaa ggcggcggcc tacgtgcggt tcgcgatcga
     5581 aaacccgcag cgctttcgcc tgatgtctcg gggcggcgtg gcggcgacca aggcctacga
     5641 ggagctggtg gatgcgttga gcgagcaggc cgacccgaag caggcaaggg tcgtcgcggc
     5701 g
//
LOCUS       DASPXW010000159         3103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_172386, whole genome shotgun sequence.
ACCESSION   DASPXW010000159 DASPXW010000000
VERSION     DASPXW010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172386"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            271..1245
                     /locus_tag="VEL04_03785"
     CDS             271..1245
                     /locus_tag="VEL04_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HYT47517.1"
                     /translation="MKAWVIAGALLAAAPAYAQIVKIDGSSTVFPITEAVAEEFQKAK
                     QNKVKVTVGISGTGGGFKKFCRGETDVSDASRPILKKEMEDCKAAGIEYFELPVAFDA
                     LTVVINPKNSFIKQLTIAEMKKMWEPAAQGKVTRWNQVNPAWPDQPMKLFGPGADSGT
                     FDYFTEAVVGKAKSSRGDFTASEDDNVLVQGVARDVNALGYFGYAYYVENKDKLKAVP
                     IVNNKGQPVLPSLEAVIKGTYQPLSRPIFIYVNAKSLGKPEVKEFVEFYLTHGAELSQ
                     EVKYVPLPTHAYKTAWDHVLKNKKGSVFGGTAEVGITIEELLKREAKL"
     gene            1254..2165
                     /gene="pstC"
                     /locus_tag="VEL04_03790"
     CDS             1254..2165
                     /gene="pstC"
                     /locus_tag="VEL04_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HYT47518.1"
                     /translation="MSAAGARLARRRSRVMREGLIEGVLFLAALVSVFTTIGIVYILL
                     KESVAFFEQVSITRFLTDTQWTPLFDDAHFGIAVLLSGTLTSSFVALAVAIPLGTVIA
                     IYLSEFAPFKLREVAKPFLELLGGVPTIVYGYFALLFVTPLLQHLYPGLPGFNLLSAG
                     IVMGIMIIPYVSSISEDAMRAVPMSLREGSYAMGATRLQTALRVVVPAALSGLASAYI
                     LGISRAVGETMIVAVAAGMQPNLTFNPLEPAATITAYIVQVALGDLPHGSIGYQTIFA
                     AGLTLMLFTLACNIAGHILRKRFHEAY"
     gene            2152..3075
                     /gene="pstA"
                     /locus_tag="VEL04_03795"
     CDS             2152..3075
                     /gene="pstA"
                     /locus_tag="VEL04_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HYT47519.1"
                     /translation="MKPTDLEEIRALIQAHKRHDALFAVIGLVALMIGVLTFAALFAD
                     MAWNGLGRLDWDFFTRYPSRRPGQAGILSAWVGSTLVMLVTACFAVPLGVAAGVYLEE
                     YARKNWLTDLIEINITNLAGVPSIVYGLLALGLFVYQFGLGQSILSAGLTLALLILPV
                     VIVATREAIRSIPQAIREGSYALGATRWQTVRYHILPYSSAGILTGIIIGMARAIGET
                     APIITIGALTFIAFLPPAPVKAEPPFVSFEWLFSPFTVMPIQMFNWTSRPEAAFQVNA
                     AAAGFVLVLMTLAMNGLAIWLRYRLRRKIKW"
BASE COUNT          533 a         1019 c          964 g          587 t
ORIGIN      
        1 agaagatcgg ctgcagatct tcccgcttca acagtaatct gtttcccagt ctttttgaaa
       61 gagcacgtgc aagctcgagc ggcgactcgg ccgccacgtc gtagacgcgc gccttgagga
      121 tccgctcgag ataatcgctg cgcttcactg tccctccccc gcagccggcg aatttagcat
      181 ggaggaaccc tcgcagcccg gacggtcaca aaccggtaac acgcatgtaa tagtctgcgc
      241 ggcgtcatcc aatccacaag gagcgtccct ttgaaagcat gggtcattgc cggtgccctc
      301 ctggccgccg cgcccgcgta cgcgcagatc gtgaaaatcg acggctcgag caccgtgttc
      361 ccgatcaccg aggccgtcgc cgaagaattc cagaaggcga agcagaacaa ggtcaaggtg
      421 acggtaggca tctcgggaac cggcggcggc ttcaagaagt tctgccgcgg cgagaccgat
      481 gtttcggatg cctcgcgtcc gatcctcaag aaggagatgg aagactgcaa agcggctggg
      541 atcgagtact tcgagctgcc ggtggcgttc gacgccctga cggtcgtgat caaccccaag
      601 aacagcttca tcaagcagct caccatcgcc gaaatgaaga agatgtggga gccggcggcg
      661 cagggcaagg tgacgcgctg gaaccaggtc aacccggcgt ggcccgacca gccgatgaag
      721 ctgttcggcc ccggcgcaga ttccggcacc ttcgattact tcaccgaggc ggtggtcggc
      781 aaggccaagt cctcgcgggg cgatttcacc gcctcggagg acgacaacgt gctggtgcag
      841 ggcgtggcgc gcgatgtcaa cgcgctcggt tatttcggct acgcgtacta cgtggagaac
      901 aaggacaagc tgaaggccgt gccgatcgtc aacaacaagg gccagccggt gctgccttcg
      961 ctcgaggcgg tgatcaaggg tacctaccag cccctgtcgc gcccgatctt catctacgtg
     1021 aacgccaagt cgctcggcaa gccggaggtg aaggagttcg tcgagttcta cctgacccac
     1081 ggcgccgagt tatcgcagga agtcaagtac gttccgctgc cgactcatgc ttacaagacg
     1141 gcgtgggacc acgtgctgaa gaacaagaag ggcagcgtgt tcggcggcac cgcggaggtc
     1201 ggcatcacta tcgaagagct cctgaagcgc gaagcgaagc tttgaaaaaa gccttgagcg
     1261 cagccggcgc ccgcctcgct cgccgccgtt cacgcgtcat gcgcgagggg ctgatcgaag
     1321 gcgtgctgtt cctcgccgcg ctggtgtcgg tgttcaccac catcggcatc gtttacatcc
     1381 tgctgaaaga gtcggtagcg ttcttcgagc aggtgtcgat cacgcgcttc ctcaccgaca
     1441 cgcaatggac gccgcttttc gacgacgcgc atttcggcat cgcggtcctg ctctccggaa
     1501 cgctgacgag ctccttcgtg gcgctcgcgg ttgcgattcc gctaggcacc gtcattgcca
     1561 tttatctttc ggagttcgcg ccgttcaagc tgcgcgaggt cgccaagcct tttctcgagc
     1621 tcctgggcgg cgtgccgacc atcgtctacg gctatttcgc gctgctcttc gtcacaccgc
     1681 tgctgcagca cctgtacccg gggctgcccg gcttcaacct gctgtcggcc gggatcgtga
     1741 tgggcatcat gatcattcct tatgtgagct cgatttccga ggacgcgatg cgggcggtgc
     1801 cgatgagcct gcgcgaaggg tcgtatgcga tgggcgccac acgcctgcag acggcgctgc
     1861 gcgtggtcgt gccggcggcg ctctcggggc tggcgtcggc gtacatcctc ggaatctcgc
     1921 gcgcggtggg cgagaccatg atcgtggcgg tcgccgccgg catgcagccc aatctcacgt
     1981 tcaatccgct cgaaccggcc gccaccatca ccgcctacat cgtgcaggta gcgctcggcg
     2041 acctgccgca cggctcgatc ggctaccaaa ccatcttcgc cgccgggctg acgctgatgc
     2101 tgttcacgct cgcctgcaac atcgcgggcc acatcctgcg caagcgcttc catgaagcct
     2161 actgacctgg aagaaatccg cgcgctgatc caagcgcaca agcgccatga cgccttattc
     2221 gcggtcatcg gcctcgttgc gctcatgatc ggggtgctga ccttcgccgc gctgttcgcc
     2281 gacatggcat ggaacggcct tggccgcctc gactgggatt tcttcaccag gtatccttcg
     2341 cgccgccccg gtcaggccgg catcctgtcg gcctgggtcg gctcgacgct ggtgatgctg
     2401 gtgaccgcct gcttcgcggt gccgctcggc gtcgccgccg gcgtgtacct cgaggagtac
     2461 gcgcgcaaga actggctcac cgatctgatc gagatcaaca tcaccaacct cgccggcgtg
     2521 ccgtccatcg tctacggcct gctcgcgctc ggccttttcg tctaccagtt cggcctgggg
     2581 cagagcatcc tgtcggccgg gctcacgctc gcgctgctca tcctgccggt ggtgatcgtc
     2641 gcgacgcgcg aggcgatccg ctcgatcccg caggcgatcc gcgagggctc gtatgcgctc
     2701 ggcgcaacgc ggtggcagac ggtgcgctac cacatcctgc cgtattcctc ggcgggcatc
     2761 ctcaccggca tcatcatcgg catggcgcgt gccatcgggg agacggcgcc gatcatcacc
     2821 atcggcgcgc tcaccttcat cgccttcctg ccgccggcgc cggtcaaggc cgagccgccg
     2881 ttcgtgtctt tcgagtggct cttctcgccc ttcaccgtga tgccgatcca gatgttcaac
     2941 tggacctcgc gcccggaggc ggcgttccag gtgaacgcgg cggccgccgg cttcgtgctg
     3001 gtgctgatga cgctcgccat gaacggcctc gccatctggc tgcgctaccg gctgcggcga
     3061 aagatcaagt ggtgagcctg gagctatgat gtccgcgccg gaa
//
LOCUS       DASPXW010000160         6099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1434460, whole genome shotgun sequence.
ACCESSION   DASPXW010000160 DASPXW010000000
VERSION     DASPXW010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6099
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1434460"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VEL04_03800"
     CDS             <1..233
                     /locus_tag="VEL04_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47520.1"
                     /translation="LALFLDHGGAQNKAGPNPSPSHLTGSGLGFLWNPSRQFAAEIYF
                     AHGHTRLPDQPGHSLQDEAIYFRLIAYPFRPD"
     gene            complement(230..994)
                     /locus_tag="VEL04_03805"
     CDS             complement(230..994)
                     /locus_tag="VEL04_03805"
                     /inference="COORDINATES: protein motif:HMM:NF017829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF928 domain-containing protein"
                     /protein_id="HYT47521.1"
                     /translation="MKTALTIGVVGAATALALLASPAAAEERNTQAAAGGSTESRTET
                     AAPPITYKAPQRGAPATRVGGGTRSVDMKVAGLSVLAPNETGYTTREKPTIYWFVSET
                     LTSPVELTITSTASLQDAATPALEITLQPPIAKGLHALALEAHGVVLKPGVEYQWFVA
                     VVSNAAQRSNDVIAGGSIKRIAQSETVQTRLKETAPANRPALYAGEGLWYDAIEDLSK
                     LISADPGNRRLREQRAALLEQVGLGDVAAYDRAAGR"
     gene            complement(1341..3284)
                     /locus_tag="VEL04_03810"
     CDS             complement(1341..3284)
                     /locus_tag="VEL04_03810"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYT47522.1"
                     /translation="MRAALTAIVMAGGLVFAAILGVRSLGALEGLELAVYDGHIRLRP
                     AAATPEPPIVLITITERDIQQLGTWPVPDGVLAKALESLSRAGARAVGLDIYRDIPVP
                     PGHEQLNAVLAANAQIVAPMLLPQRDKAGVAPPRVLKGTDRVGFTDIVVDADGTVRRG
                     LLLMDDGSDVFYSLPLRVALLYLGATGIALEGDAENPTHIRLGRTTLRPFEPNDGSYV
                     RADAGGYQFLLDYREPPEAFPSITFGRLLAGEFDAALIKDRIVLVGVTAESVKDNFYT
                     PYSGTFGEKRTTYGVALYAQIVSQLLRAAIAGAAPLAVLSDTAEALWMLLWSLLGAAV
                     PILVRSAWRFALAVAAGLVLLALAVHGLFVLGWWFPLVPSALGWLAAAGLVTAYMSSQ
                     EKKERGKLMSLFSSYVSADLAEAIWREREQFVSGGRPTPQRLTATVLFSDVGGFTTIS
                     ETLEPQTLMAWLYDFMEATTPIVSAHDGVIFRFIGDSIMAVFGVPVARTSEKAIARDA
                     MNAVDCALAIQSRLVTLNASLAQRGLPLIGMRIGILTGPMVAGSLGSAERLEYNVHGD
                     TVNTAARLESFDRERFAPDYLQAPCRILVGEPTLQLLGGEFHSEFVGEFQLKGKAKAI
                     RIHRIYGRNGASELRRAAGPADN"
     gene            3353..5503
                     /locus_tag="VEL04_03815"
     CDS             3353..5503
                     /locus_tag="VEL04_03815"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HYT47523.1"
                     /translation="MNAAALALCFLLFAWPPAAGADEAAGNARAQVQALMRGAEADLA
                     VGRNPGALDKLERALPLAQRSGDEPLLAAVEASLGSAYALAGRFADAERSLRSSIERA
                     RKANHPQAAAVALNSMGNLLAAQARFGEAQKIYREALKAAQGAGSRSLAARASTNLAR
                     ALMDDGRDREASALLPPLHEDLRALESSRDKAYGLISLGRLYARLERSRAAYAALNEA
                     LGAAEAVGDARARSSALGYLGELYEQAARHAEALQFTQRAILAAEEADAPELLYRWQW
                     QQGRLLKAQGHRDQAILAYQHAVYTLAVVRPDLAASPARGSFRETVGPVFSQLADLLL
                     EHSATLDDAKLVTLALADARNTMELLKGAELQDYFQDDCVTALRARTTGIDQLAPRTA
                     ALYPIIFKDRLELLLTLPDGMKRFTTRVDADTLTKEIRAFRALLEKRTTHQYFPHARK
                     LYDWLIRPIAAELEKQGVETLVVVPDGPLRTIPIAALHDGEQFLIARYALATTPGLTL
                     TDPRPLPRERMQLLLNGLTDGVQGFPPLPYVAQELDAIHKLYGGTVLQNGQFTLSNME
                     KGLEETPYSIVHIASHGQFDSDVDKTFLLTYEGKLSMSKLEQFLGLSKFRTDAVELLT
                     LSACQTAAGDDRAALGLAGVAVKAGARSAIATLWTVNDPASAVIVSDFYRELRNPAVS
                     KAKALQQAQLGALRDLRYRHPNYWSAFLLIGNWL"
     gene            complement(5593..>6099)
                     /locus_tag="VEL04_03820"
     CDS             complement(5593..>6099)
                     /locus_tag="VEL04_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47524.1"
                     /translation="DAIKTSLSGRYGDMTLSRRRTALPECGVIAYDVPGTAQGNWFRS
                     DLPLSSSNADGLLAFVFDNIDPTIPVISVGRTNNDNIPPLVAYTFTPNPSVGYVRQPF
                     AGVQNDGHKYCYDGLVFYSGGFGLVSGRIIVEVTGAESLRIEKQASDCSGDPALWTFG
                     PDATDYIR"
BASE COUNT         1004 a         2087 c         2041 g          967 t
ORIGIN      
        1 agctcgcgct gttcctcgac cacggcggcg cgcagaacaa ggcggggccg aacccgagcc
       61 cgtcgcacct cacgggaagc gggctcggct tcctctggaa cccgagccgg cagttcgccg
      121 ccgagatcta cttcgcccac ggccacacca ggctgcccga ccagcccggg cacagcctgc
      181 aggacgaggc gatctacttc cggctgatcg cctatccctt caggccggac tagcggcccg
      241 ccgcgcggtc gtaggccgcc acgtcgccca ggccgacctg ctccagcagc gccgcgcgct
      301 gctcgcgcag ccggcgattg cccgggtcgg cgctgatcag cttcgacagg tcctcgatgg
      361 cgtcatacca gaggccctcg cccgcgtaga gtgccggacg gttggcgggc gcagtctcct
      421 tgagcctggt ttgcacggtt tcgctctggg cgatgcgctt gatcgagccg ccggcgataa
      481 cgtcgttgga gcgctgcgca gcgttgctca ccaccgcgac gaaccactgg tactcgacgc
      541 cgggcttgag caccacgccg tgcgcttcga gcgccagcgc atgcagccct ttggcaatcg
      601 gcggctgcaa ggtgatctcc agggccggcg tcgcggcgtc ctgcaggctg gcggtgctcg
      661 tgatcgtcag ctcgacggga ctggtcagcg tctcggaaac gaaccagtag atggtcggct
      721 tctcgcgggt cgtgtagccg gtctcgttgg gcgcgagcac gctgaggccg gcgactttca
      781 tgtcgacgct gcgcgtgccg ccgccgacgc gagtcgcagg cgcgccgcgc tggggcgcct
      841 tgtaggtaat gggcggcgcg gcggtttcgg ttctgctttc cgtgctccct ccagccgcgg
      901 cctgcgtatt gcgttcttcc gccgcggccg gcgaagcgag aagcgccagg gccgtagccg
      961 cgcccacgac gccgatggtc agtgctgttt tcatagttcc tccgctttgc tgcttatctt
     1021 cccgtcaagc ctacgcggat gggagagccg tgtcagcgcc tggcggcact cgccggctgt
     1081 gcgaaattca cctccccggc gggggatatc ccacaggcgg cgagcgcctt gcttcactgc
     1141 agcgcaggcg ccccgccctc tacagtgggc ccatgttcaa aggaggagcc atgacgatcc
     1201 gcaaaagcct cgccttgctc gccaagctga tggagtccag cgccgcctcg ggccgcaggc
     1261 ttcccgccgg ccgctgcgca tgccacgaat ccacggtcaa gtggtgcacg cgccacggag
     1321 agccgatgcc gcgcggcccc tcagttgtca gcggggccgg cagcgcgccg tagctcgctc
     1381 gcgccattac ggccgtagat gcggtggatg cggatcgcct tcgccttgcc tttgagctgg
     1441 aattcgccga cgaactcgct atggaactcg ccgcccagca gctgcagcgt cggctcgccg
     1501 acgagtatcc ggcagggcgc ttgtaggtaa tccggcgcga agcgctcccg gtcgaagctt
     1561 tccagccgcg cggcggtatt cacggtatcg ccgtgcacgt tgtactcgag acgctcggcg
     1621 ctgccgaggc tgccggcgac catcgggccg gtcaggatgc cgatacgcat gccgatgagc
     1681 ggcaggccgc gctgcgcgag gcttgcgttg agcgtaacca gccgcgactg gatggcgagg
     1741 gcgcaatcga ccgcgttcat cgcgtcccgt gcgatcgcct tttcgctcgt gcgcgccacc
     1801 ggcacgccga acaccgccat gatggaatca ccgatgaagc ggaaaatcac gccgtcgtgg
     1861 gcgctgacga tcggggtggt ggcctccatg aagtcgtaga gccaggccat gagcgtctgg
     1921 ggctccaaag tctctgaaat ggtggtgaag ccccctacgt cggagaaaag cacggtggcg
     1981 gtgagccgct gcggcgtggg gcgcccgccg ctcacgaact gctcgcgctc ccgccagatc
     2041 gcctcggcga gatccgccga gacatagctc gagaagaggc tcatcagctt gccgcgctcc
     2101 ttcttctcct ggctcgacat gtaagcggtc accagtccgg cggcggccag ccagccgagc
     2161 gcggacggca cgagaggaaa ccaccagccg agcacgaaca ggccgtgcac cgcgagcgcc
     2221 agcaggacca gcccggccgc gaccgcgagc gcgaagcgcc acgccgagcg cacaaggatc
     2281 ggcacggcgg cgccaagcag gctccacagc agcatccaca gcgcctcggc cgtgtcgctc
     2341 agcacggcga gcggcgcagc acccgcgatg gccgcgcgca agagctggct gacgatttgc
     2401 gcgtagagcg cgacgccgta cgtggtgcgc ttttcaccga acgtcccgct gtagggcgtg
     2461 tagaagttgt ccttcacgct ctcggcggtg acgccgacca gcacgatgcg gtccttgatc
     2521 agcgccgcgt cgaattcgcc cgcgagcagc cggccgaaag tgatcgaggg aaacgcttcc
     2581 ggcggctcgc gatagtcgag caggaattga tagccgcccg cgtcggcccg cacatagctg
     2641 ccgtcgttcg gctcgaacgg gcgcagcgtc gtgcgcccca ggcggatatg ggtcgggttt
     2701 tccgcatcgc cctcgagggc gatgccggtg gccccgaggt agagcagcgc gacgcgcaag
     2761 ggcagcgagt agaagacgtc gctcccgtca tccatgagga gcaggccgcg gcgcacggtc
     2821 ccgtccgcgt cgacaacgat gtcggtgaag cccacccggt ccgttccctt cagcacgcgc
     2881 ggcggcgcga cgccggcctt gtcgcgctgc ggcagcagca tcggcgcgac gatctgcgca
     2941 ttggcggcca ataccgcgtt cagctgctcg tgtccgggag gcacggggat gtcgcgatag
     3001 atgtcgagcc cgacggcgcg cgcgccggcg cgcgaaaggc tctcgagcgc ctttgccagc
     3061 acgccatccg gaaccggcca ggtgccgagc tgctggatat cgcgctcggt gatcgtgatc
     3121 agcacgatcg gaggttcggg cgtggcggcc gcaggcctga gccggatatg cccgtcgtag
     3181 acggccagct cgaggccctc gagcgctccc aggctgcgca cgccgaggat ggcggcaaag
     3241 accaggccgc ccgccatcac gatcgccgtc aacgcagcac gcacccgccg taccttattc
     3301 gcacgcgata ggcgcagaat agaggaaact cgcgctccgg gagggggctc gcatgaacgc
     3361 cgcggcactg gcgctgtgtt tcctgctgtt cgcatggccg cccgccgccg gcgcggacga
     3421 ggcggccggc aacgcacggg cgcaagtcca ggcgctgatg cgcggcgccg aggccgatct
     3481 ggccgtcggc cgcaatcccg gcgcgctcga caagctggag cgcgctctgc cgcttgcgca
     3541 gcgcagcggc gacgagccgc tgctggcggc ggtggaggcg agcctcggga gtgcctacgc
     3601 gctcgccggg cgcttcgccg acgccgagcg atcgctgcgc tcgagcatcg agcgcgcccg
     3661 caaggcgaac cacccgcagg ccgctgccgt ggcgctcaac agcatgggaa acctgctcgc
     3721 agcgcaggcc cgcttcggcg aggcgcagaa aatctatcgc gaagcgctca aagcggcgca
     3781 gggcgcgggc agcaggagcc tcgcggcgcg cgcttccacc aacctcgcgc gcgcgctgat
     3841 ggacgatggc cgggatcgcg aggcgagtgc cctgctgccc cccttgcacg aagacctgcg
     3901 cgcgctcgaa tcctcgcgcg acaaggcgta cgggctgatc agcctcggac ggctgtacgc
     3961 gaggctcgag cgcagccgtg ccgcgtatgc cgccctgaac gaagcgctcg gcgcggcgga
     4021 ggcggtcggc gatgcgcgcg cgcgctcctc agcgctcggc tatctgggcg agctctacga
     4081 gcaggccgcg cgccacgccg aggcgctgca attcacgcag cgtgccatcc ttgccgccga
     4141 ggaggccgat gcgccggagc ttctctatcg ctggcaatgg cagcagggac ggctgctcaa
     4201 ggcgcaggga cacagggacc aggcgatcct cgcgtaccag catgccgtgt acacgctcgc
     4261 cgtcgtgcgc ccggacctgg cggcgagccc ggcgcgcggc tcgtttcgcg agacggtggg
     4321 gccggtcttc tcgcagcttg ccgaccttct gctggagcat tcggcgacgc tcgacgatgc
     4381 gaagctcgtc acgctcgccc tcgccgatgc gcgcaatacc atggagctcc tgaagggcgc
     4441 ggagctgcag gattacttcc aggacgactg cgtcaccgcc ctgcgcgcac gcacgaccgg
     4501 catcgatcag ctcgcgccgc ggaccgcggc gctctatccg atcatcttca aggaccggct
     4561 cgagctgttg ctcacgctgc ccgacgggat gaagcgcttc acgacgcgcg tcgacgccga
     4621 cacgctcacc aaggaaatcc gcgcgtttcg cgcgctgctc gagaaaagga ccacgcacca
     4681 gtatttcccg cacgcgcgca agctctacga ctggctgatc cggccgatcg ccgccgagct
     4741 ggaaaagcag ggggtcgaga cgctggtggt ggtgcctgat ggaccgttgc gcacgatccc
     4801 catcgccgcc ttgcacgacg gcgagcaatt cctgatcgcg cgctacgcgc ttgccacgac
     4861 tccagggctg acgctcacgg atccgcggcc gctaccgcgc gagcgcatgc agctcctgct
     4921 gaacggcctg accgacgggg tgcagggctt cccgccgctt ccctacgtcg cgcaggagct
     4981 cgacgccatc cacaagctct acggcggcac ggtgctgcaa aacggccagt tcaccttgtc
     5041 gaacatggag aagggcctcg aggagacgcc gtattccatc gtgcacatcg cctcgcacgg
     5101 gcagttcgac agcgatgtcg acaagacctt cctcctgacc tacgagggaa agctcagcat
     5161 gagcaagctg gagcagttcc tcgggttgtc gaagtttcgc accgatgcgg tcgagctcct
     5221 gacgctgagc gcctgtcaga ccgcggcagg cgacgaccgg gcggcgctcg gcctcgccgg
     5281 cgtggcggtc aaggcgggcg cgcgcagcgc gatcgccacc ctgtggaccg tcaacgatcc
     5341 ggcctcggcg gtgatcgttt cggacttcta ccgcgagctg cgcaatccgg ccgtctccaa
     5401 ggccaaggcg cttcagcagg cgcagctcgg cgctttgcgc gacctgcgct accgccaccc
     5461 gaattactgg agcgcgttcc ttctgatcgg caactggctt tagcggcgct agttcgtcgt
     5521 gcgcccgctt catgaacttg cctcttgctc gccgggcggg ccgtattccg ggcatagaga
     5581 gcaggcttag ttctatcgta tataatcggt cgcgtctgga ccgaacgtcc aaagcgccgg
     5641 gtctccggaa cagtcgcttg cttgtttttc tatccgcagg ctttcagctc cagtcacctc
     5701 gacaatgatg cggccagaga ctaagccgaa acccccgctg taaaagacaa gcccgtcgta
     5761 gcagtacttg tgtccatcat tctggactcc ggcgaagggc tgtctcacgt agccaacgct
     5821 cggattgggc gtgaacgtat aggccactaa aggcggaata ttatcgttat tcgtcctgcc
     5881 gacggaaatc accggtatgg tggggtcaat gttatcaaag acaaaagcaa gaagtccgtc
     5941 cgcgttagag gaggacagcg gaaggtcgga ccggaaccag ttgccctggg cagtccccgg
     6001 cacgtcatag gcaatcacgc cgcattcagg tagcgccgta cgtcgccgcg aaagggtcat
     6061 gtccccgtat ctgcctgaga gcgatgtttt gatcgcatc
//
LOCUS       DASPXW010000161         4046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_426173, whole genome shotgun sequence.
ACCESSION   DASPXW010000161 DASPXW010000000
VERSION     DASPXW010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426173"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VEL04_03825"
     CDS             <1..744
                     /locus_tag="VEL04_03825"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HYT47525.1"
                     /translation="RNLVAERTQGTLPQFKRIVIETSGLADPGPILQTFATDRALGGE
                     FHVEVVVAVVDAVGGLDTLAWSVEARKQVILADRLVVSKTDLAPSQAVKRLTARLRAL
                     NPHAAILTAVDGDLDPRCLVEPDAHFPACVRSGFVAEGEHSDGILSFVLTQDAPLPWE
                     AFARAMETLIALRGADLLRVKGLLNVAGCRGPVVVHVVQHLAHPPVELAAWPDKNHAS
                     QLVFITRGIAERHVRDLFASVHALAGDPS"
     gene            complement(754..945)
                     /locus_tag="VEL04_03830"
     CDS             complement(754..945)
                     /locus_tag="VEL04_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47526.1"
                     /translation="MINATEYLAMAAEHHRLAGICRSPDSREQHLRLEEQFRALAERG
                     ETSHEQPAPQPARDPQIVK"
     gene            complement(1332..3527)
                     /locus_tag="VEL04_03835"
     CDS             complement(1332..3527)
                     /locus_tag="VEL04_03835"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HYT47527.1"
                     /translation="MKSRYRSALVAALACASALAITLAAETAAARGAKGAHATAKVAG
                     KTTKATGKKVAGKKGAGKSKGSKSAEDKSAVPQIPLAQGPAPQTSAADIALVKSAVEA
                     LRRGGASKATEVAGGISDPLARKLVEWLILRSDHNGAESKRYLAFIAANPSWPNLGMF
                     RKRAEGMFWVENVKPAQVVSFFKDSPPQSGVGRMVLARALHAQGDAEGAAALVRQAWR
                     NDSLPEDVEKQVLERWSASLSRADHKARMEKRLFAADKDDAMRAARRLGGADLAIAQA
                     RIALHRKVGAPKLEPKSARKLLDAVPADARQDPGYLFAHAHVLRHEGKIAEAAQVLLS
                     APCDLAEIHDGEEWWVERRIMARKLLDMGDAQSAYRVVADAAEPNKENSRIERHFMAG
                     WIALRFLDDPATAATHFKRIQDVTRHPTSHARSHYWLGRVAEALHQPEQARAEYETAA
                     RGSAAYYGQLARARLGLPALALAPPPPKPDPQAEAERLELVRALEILYALKERQLVIT
                     LMASIGESHNDAGTLSALGELAEQYEDARGMLHMGKGALARGLPLDHYAFPTVGVPRY
                     SPIAPSIESAMLFAIIRQESAFDPSDWSHAQAMGLMQVTPEAGRDTCKRFNCKYDFKR
                     LRTDSPYNLQVGAAEVAGVLQDYRGNQLLAFAAYNAGRGRVQEWIASYGDPRDPTVDP
                     VDWVERIPFMETRNYVQRVMENFQVYRVRFANDAPLTIDKDLRRDAAAR"
BASE COUNT          642 a         1374 c         1288 g          742 t
ORIGIN      
        1 cgcaatctcg tcgccgagcg cacgcaaggc acgttgcccc agttcaagcg gatcgtgatc
       61 gagaccagcg ggcttgccga tcctggaccg atcctgcaga cgtttgccac cgatcgggcg
      121 ctgggcgggg aatttcacgt cgaagtggtc gtcgccgtcg tcgacgccgt aggcggcctc
      181 gatacgctcg catggtcggt agaggcacga aagcaggtca tcctggctga tcgactggtc
      241 gtttcgaaga ccgacctcgc cccgtcgcaa gcggtcaagc gcctgaccgc gcgccttcgc
      301 gcgctcaatc cccacgcggc cattcttacg gcggtcgacg gcgatctcga tccccgctgc
      361 ctggtcgagc ccgatgcgca cttccccgct tgtgtgcgct ccggcttcgt cgccgaaggc
      421 gagcatagcg acggcatttt aagtttcgtt ttgacgcagg acgcgccgct gccttgggag
      481 gccttcgcgc gcgctatgga aacgctgatc gcgctgcgcg gagccgacct cctgcgtgtg
      541 aaggggttgc tcaacgtcgc gggctgccgc gggccggtgg tggtgcacgt ggtccagcac
      601 ctcgcccatc cgccggtcga gctagcggcc tggcccgaca agaaccacgc aagccagctc
      661 gtgttcatca cgcgcggcat tgcggagcgg cacgtgcgcg acctctttgc ctcggtgcac
      721 gctttggccg gcgatcccag ctagatactg gcgctatttt acgatctgcg gatcgcgtgc
      781 cggctgcggc gcgggctgtt cgtgcgaagt ttccccacgc tcggcaagcg cccggaattg
      841 ctcctccaga cgcaagtgct gttcacgcga gtcgggtgag cggcatatgc cggccaggcg
      901 atggtgttcc gcagccatcg ccagatattc ggttgcgttg atcatcgtat cctccccacg
      961 cgcaagtcca gtctgccccg gtcgagccgg agcaaacggc gtgggacgct agaacgggtc
     1021 ccatgaacaa gttgtgacat gaaatcccga acgctttgcg ctggcgtttc gagcgcatcc
     1081 agtctgcgcc tttgcggaga caaagtcgag ccctcgcgtc tgggcttgtc gctctagccc
     1141 ggattgagcg aagcggaatc cgggacatca cgcgggtagt caggtgggtc tggccgccgt
     1201 caccgtccag ccttggccgt cgctcccgcg cattgaacgg gcagagcccg gacgccgcca
     1261 tagcgcgtcg aagactcgcg cgtaaacgcg cttatggctc cgtccgggct acgcagcaag
     1321 gctggtggta attagcgcgc cgcggcgtcg cgccgcaggt ccttgtcgat cgtcagcggc
     1381 gcgtcgttgg caaaacgcac gcgatagacc tgaaaattct ccatcacccg ctgcacgtag
     1441 ttgcgcgttt ccatgaacgg gatgcgctcg acccagtcga cggggtcgac cgtggggtcg
     1501 cgcggatcgc cgtagctcgc aatccactcc tgcacgcgcc cgcgccccgc attgtaggcc
     1561 gcaaaggcca ggagctggtt gccgcgataa tcctgcagca cgccggcgac ctcggcggcg
     1621 ccgacctgaa ggttgtacgg cgagtcggtg cgcaggcgct tgaagtcgta cttgcagttg
     1681 aagcgcttgc aggtatcccg tccggcttcc ggcgtcacct gcatcagacc catcgcctgc
     1741 gcgtgcgacc agtcggaggg atcgaacgcg ctctcctggc gaatgatggc aaacagcatc
     1801 gcgctctcga tgctgggggc aatcggcgaa tagcgcggca cgccgacggt cggaaacgcg
     1861 tagtggtcga gcggtaagcc gcgcgccagc gcacccttgc ccatatgcag catcccacgg
     1921 gcgtcctcat actgctccgc caattcgccc agcgccgaga gcgtacccgc gtcgttatgc
     1981 gactccccga tgctggccat cagcgtgatg accaactggc gctccttcag cgcgtagagg
     2041 atctccagcg cccgaacgag ctccagccgt tccgcctcgg cttgcgggtc cggcttcggc
     2101 ggtggcgggg ccagcgcgag cgctggaagc cccagccgcg cccgggcgag ctgcccgtaa
     2161 taggcggccg aaccgcgcgc cgccgtctca tactcggcgc gggcctgctc tggctgatgc
     2221 aaagcttccg ccacacgccc gagccagtag tgcgagcgcg cgtgcgaggt cggatggcgg
     2281 gtcacgtcct ggatgcgctt gaagtgggtt gccgccgtgg ccggatcgtc gaggaaccgc
     2341 agcgcgatcc atcccgccat gaaatggcgt tcgatccgcg agttttcctt gttcggctcg
     2401 gccgcgtccg ccaccacccg ataggccgat tgggcatcgc ccatatcgag cagcttgcgc
     2461 gccatgatgc gccgctcgac ccaccattcc tcgccatcat ggatttcggc aaggtcgcag
     2521 ggcgcagaca gcaagacctg cgccgcttcg gcgattttgc cctcgtggcg cagaacgtgc
     2581 gcatgcgcga acagatagcc gggatcctgc cgcgcgtccg ccggcacggc gtcgagcagc
     2641 ttcctggcac ttttcggctc gagcttgggc gcgccgacct tgcggtggag cgcaatgcgc
     2701 gcctgcgcga ttgcgaggtc ggccccgccc agccggcgtg ccgcccgcat cgcgtcatcc
     2761 ttgtcggcgg cgaacagtcg cttttccatg cgcgccttgt gatcggcgcg gctcagcgac
     2821 gcggaccaac gctcgagcac ctgcttctcg acatcctcgg gaagcgaatc attgcgccaa
     2881 gcctgtcgca ccagcgcggc cgcgccttcg gcatcgccct gcgcgtgcag cgcgcgcgcc
     2941 agcaccatgc ggcctacgcc cgactgcggc ggcgagtcct tgaagaaact tacgacttgc
     3001 gccggcttga cgttttcgac ccaaaacatc ccctccgcgc gcttgcggaa catgccgagg
     3061 ttcggccagc tcggattggc ggcaatgaag gcgaggtagc gcttgctctc ggcgccgttg
     3121 tggtcactgc gcaagatgag ccattccacg agcttgcgcg ccagcgggtc tgagatgccg
     3181 ccggcgacct cggtcgcctt gctggcgcca ccacggcgca gcgcctcgac tgcgctcttg
     3241 accagcgcaa tgtcggcggc ggacgtctgc ggcgccgggc cttgcgccag cggaatctgc
     3301 ggcaccgcgg atttgtcttc tgccgatttg ctacccttgg atttgcccgc ccccttcttt
     3361 ccggccacct tctttccggt ggctttcgtg gttttacccg cgaccttggc cgtcgcgtgc
     3421 gcgcccttgg cgccgcgcgc agccgccgtc tccgccgcaa gggtgattgc gagcgcactc
     3481 gcgcaggcga gtgcagcaac gagtgctgac cgatatcgtg atttcaagat ttgtccccag
     3541 atcccccggc gaatcgcttc gccccaagtc caactctggt acccgaccac ccgaattgag
     3601 cagcgctatg cagcaacatt tgggcggaaa agtgccgcta ttgtcgccaa gcagtagccc
     3661 ggacggagcg aaacaacctc tgaaatccga agcgaaatcc gcgattcacg gcgaacccgc
     3721 ctgagagctc ggccacgaca cgcaccgctc ctgatcgtgc gccccccgtc atactcgcat
     3781 gcgattagaa cctggatgtc gattcgctcc attcaggcta cgcgctctct ttctgcggtc
     3841 gtccccgcga agcggggcac ccgtgaagac gggcctgtgt tcctcgggtc ccggctttcg
     3901 cgcttcccgc tcggccggga cggcaagcat ggataagcag cgagctacga attggcatcg
     3961 aggtcggact ccgattacga tgcgtgcgga cgttttcggc gtggtgccgt atttcgcgaa
     4021 cttgcaaggg agacgcgtat gtccgc
//
LOCUS       DASPXW010000162         2534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1559358, whole genome shotgun sequence.
ACCESSION   DASPXW010000162 DASPXW010000000
VERSION     DASPXW010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1559358"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /gene="coaE"
                     /locus_tag="VEL04_03840"
     CDS             <1..507
                     /gene="coaE"
                     /locus_tag="VEL04_03840"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668272.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HYT47528.1"
                     /translation="AHELTAPEGAALPHIKSLFGEAFILSSGAMDRGKMRERVFSDPA
                     AKRALEALLHPMIRDESGWRIAAATGPYVIHVVPLLIESPGYRNRVDRVLVVDCPENT
                     QVARARARSSLSDDEVRAIIATQVSRAQRLAAADDVIDNSGTLDALRQQVTALHQKYL
                     EIGRTSRS"
     gene            577..1335
                     /gene="zapD"
                     /locus_tag="VEL04_03845"
     CDS             577..1335
                     /gene="zapD"
                     /locus_tag="VEL04_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006756831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HYT47529.1"
                     /translation="MITYEYPLNERIRTLLRLEDLFERSRHFIARSDPHDHHMALITL
                     FEILEVASRADLKSDLLQELERQKQVLLAFRNNPDIAENALASVLRDIEQAAAALFSM
                     TGKIGQYLRGNDWLMSIKQRTGIPGGACEFDLPSYHYWLHRPPDERTGQLAAWTGPLH
                     PLRDGSAIILRILRESGRPQTLSASQGMFQQMLGGKTAQMLRLRLDPATPCVPEISAN
                     KYVLNIRFMAQNGEDPRPRTADWNVAFELTFCNL"
     gene            1335..1538
                     /locus_tag="VEL04_03850"
     CDS             1335..1538
                     /locus_tag="VEL04_03850"
                     /inference="COORDINATES: protein motif:HMM:NF015821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HYT47530.1"
                     /translation="MERTRRVACPRCGAMNLFTPDNEWRPFCSERCKMIDLGLWGSDG
                     YRIPDKQAGSEADADNKTGSQPD"
     gene            complement(1535..2488)
                     /locus_tag="VEL04_03855"
     CDS             complement(1535..2488)
                     /locus_tag="VEL04_03855"
                     /inference="COORDINATES: protein motif:HMM:NF006530.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nudix family hydrolase"
                     /protein_id="HYT47531.1"
                     /translation="MPGTGLAGIRGNAEVVEVAAAVIQRADGAFLLARRPAGKVYAGY
                     WEFPGGKVEPGEPAAQALARELREELGIDVLRAYPWITRVFTYPHATVRLGFYRVFHW
                     KHQPHPREDQAIAWQRLDAPMAAPMLPANAPVLASLALPWEYAVTNAAQLGTAQMLAL
                     LGRRLGQGLKLLQIREPELAEEERKLFTRQGIGLAHRHGCRVLVKSPFPGADGIHFTA
                     AQLMKLQERPAVPLAAASCHSREELERAMRLELNFAVLGPVKQTASHQAGSTLGWDGF
                     AQVAGGASIPVYAIGGLTRRDLDDAWRAGAHGLAMVRGSWA"
     gene            complement(2448..>2534)
                     /locus_tag="VEL04_03860"
     CDS             complement(2448..>2534)
                     /locus_tag="VEL04_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF815 domain-containing protein"
                     /protein_id="HYT47532.1"
                     /translation="WSLQRGSRSGRVAYQFARDWAGRHSRKR"
BASE COUNT          452 a          866 c          789 g          427 t
ORIGIN      
        1 gcgcacgagc tgactgcgcc cgaaggtgcc gcgcttcctc acatcaagag cctgttcggc
       61 gaagccttta tcctttcctc cggagccatg gaccgcggca agatgcgcga gcgggtgttc
      121 tccgacccgg cggcgaaacg ggcgctggag gcgctgttgc acccgatgat ccgcgatgaa
      181 tcgggctggc gcatcgctgc tgcgaccgga ccttacgtta tccacgtcgt gccgctgctc
      241 atcgagtcgc cgggctaccg caaccgcgtc gatcgcgtgc ttgtcgtcga ttgcccggag
      301 aacacgcagg tcgcgcgcgc tcgcgcgcga agcagtctct ccgatgacga agttcgtgcg
      361 atcatcgcaa cgcaggtatc ccgggcgcag cggctggccg ccgccgacga cgtgatcgac
      421 aactctggaa cccttgatgc actgcgacaa caggtgaccg ctcttcatca gaaatatctc
      481 gaaatcggtc gaacttcacg gagttaggtt gtgccgcgaa cgcaaatcgc tcagaatccg
      541 ccaagctttt tgccgcgcgc gagcggcgaa gattccgtga ttacctacga atatccgctc
      601 aacgagcgca tccgcacgct gctgcggctc gaggatctgt tcgagcgctc gcgccacttc
      661 atcgcgcgca gcgatccgca tgaccatcac atggcgctga tcacactgtt cgagattctc
      721 gaagtcgcaa gccgcgccga cctgaagtcc gacctgctgc aggagctcga gcgccagaaa
      781 caggtgctgc tcgcgttccg caacaacccg gacatcgccg aaaacgctct cgcgagcgtg
      841 ctgcgcgaca tcgagcaggc tgcggcggca ctgttttcga tgaccggcaa gatcgggcaa
      901 tacctgcgcg ggaacgactg gctgatgtcg atcaagcagc gcacgggaat tccaggcggc
      961 gcgtgcgagt tcgacctgcc ttcgtaccac tactggctgc accggccgcc cgatgagcgc
     1021 accgggcagc ttgccgcctg gaccgggccg ctccatccgt tgcgcgacgg ctcggcgatc
     1081 atcctcagga tcctgcgcga atcgggccgc ccgcagaccc tgagcgcgtc gcagggaatg
     1141 ttccagcaga tgcttggcgg caagactgcg cagatgctgc gcctgcgcct cgatccggcg
     1201 acgccgtgcg ttcccgagat cagcgccaac aagtacgtgc tgaacatccg cttcatggcg
     1261 cagaacggcg aagatccgcg tccccgcact gccgactgga acgtcgcctt cgagctcacc
     1321 ttctgcaatc tttgatggag aggactcgca gggtggcgtg tccaagatgc ggcgccatga
     1381 atctctttac ccctgacaac gagtggcggc cgttctgcag cgagcgctgc aagatgatcg
     1441 acctcggcct ctggggctcc gacggctacc gaattccgga caagcaagcg gggtccgagg
     1501 ccgatgcgga caataaaacg ggctctcaac cggattaagc ccagctccct ctgaccatcg
     1561 ccaggccgtg tgcgcctgcg cgccaggcgt catcgagatc tctgcgcgtc aatccgccga
     1621 tggcgtagac cgggatactc gcgccgcctg ccacttgcgc gaatccgtcc catcccagcg
     1681 tagagccggc ctggtgagac gcggtctgct tcaccggccc gagcactgcg aaattgagct
     1741 cgagccgcat cgcccgctcg agctcttcac ggctgtgaca cgacgcggcg gcaagcggga
     1801 cagctggtct ttcctgaagc ttcatcaact gcgccgcggt gaagtgaatc ccgtccgcgc
     1861 cggggaacgg cgacttcacc agcaccctgc agccgtggcg atgggcgagc ccgatcccct
     1921 gccgggtaaa gagtttgcgc tcctcttccg ccagctctgg ctcgcgaatc tgcagcagct
     1981 tcaggccttg cccgagccgc cgcccgagca gggccagcat ctgcgcggtg ccgagctgcg
     2041 cggcgttggt gacggcgtat tcccacggca gcgcgagcga agcgagcacc ggcgcgttcg
     2101 cgggcagcat cggcgcggcc atcggcgcgt caagcctctg ccaggcgatc gcctggtcct
     2161 cgcgcggatg aggttggtgt ttccaatgaa aaaccctgta gaacccaagg cgcacggtgg
     2221 cgtgtggata ggtgaacacc cgcgtgatcc acggatacgc acgcagcacg tctatgccga
     2281 gctcctcgcg cagctcgcgc gcgagcgcct gcgccgcagg ctcgccgggc tccaccttgc
     2341 cgccggggaa ttcccagtag cccgcgtaga ccttgcctgc cgggcgccgg gcgagaagaa
     2401 acgcaccgtc ggcgcgctgg atcaccgcgg cggcgacttc tacgacttca gcgtttcctc
     2461 gaatgccggc cagcccagtc cctggcaaat tgatacgcga cccgcccgct gcgcgagccg
     2521 cgctgcagcg acca
//
LOCUS       DASPXW010000163         2107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_552978, whole genome shotgun sequence.
ACCESSION   DASPXW010000163 DASPXW010000000
VERSION     DASPXW010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552978"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2107
                     /locus_tag="VEL04_03865"
     CDS             <1..>2107
                     /locus_tag="VEL04_03865"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYT47533.1"
                     /translation="PLSYFQKNSSAQIIPMITVECEQLGGFIGDAFNLPLFQGGQLLT
                     IIFFMFVQDPVLGVAAIALYPVQGYVIPKLQFKVNQLQKRRVRTIRQVADRVQESAAG
                     IVEIQANDTGKLRLTDFAHILGVIYDIRFEIYRRKFFAKFLNNFIGQLTPFFFYSLGG
                     YLVIQGDLSFGALVAILAAYKDLASPWKELLDFYQIKENSRITYEQIVEQFQPSGMSD
                     ARLLLEEPAVVAPLTGELAVANLSLSEDDKSRIVDAVSFTVRLDEHVAVIGQSGSGKS
                     ELALMLARLVRPSSGRITIGGLDLAELPVAVVGRRIGYVGAMPYLFAGTLRDNLLLGL
                     QHRPLRPAEYDEDEARKRARQLFEAQRSGNIDFDLHADWIDYESAGVSDRDGLMHRIS
                     EVLARLDFEEDVYGLGLRGRLDPASQPELAERLLEARKALAARLVSDDITKLVETYDL
                     ARYNSNASVAENVLFGTPIGPAFDFEALADNVHVLRVLDQLELTGDLVEAGREVAATM
                     TEMFAGLPPEHEFFEQFSFIDANDLPEFAAILSAAENGGTAALAQEQRHKLLSLPFKL
                     VAARHRLGVLDERMQQRLLEARRVFRETLPEEARDQIEFFDPDRYNAAASLQDNILFG
                     KIVTGQAEAGTRITRLVRELLDELGLRPLVVEIGLDYQVGVGGARLHAADRQKTAIVR
                     ALLKRPVFIVLDHAAAVLDP"
BASE COUNT          352 a          704 c          669 g          382 t
ORIGIN      
        1 ccgctgtcct atttccagaa gaactcgtcg gcccagatca tcccgatgat cacggtcgaa
       61 tgcgagcaac tgggcggctt tatcggcgac gccttcaatt tgccgctgtt tcagggcggc
      121 cagctgctga cgatcatttt cttcatgttc gtgcaggatc cggtgctcgg cgtcgcggcg
      181 atcgcgctgt atccggtgca gggttatgtc atccccaagc tgcagttcaa ggtgaaccag
      241 ctgcagaaac gccgggtacg cacgatccgt caggtcgccg accgggtgca ggaatcggcg
      301 gccggcatcg tcgagatcca ggccaatgat accggcaagc tgcggctgac cgactttgcc
      361 cacatcctgg gcgtcatcta cgatatccgg ttcgagatct accggcgcaa atttttcgcc
      421 aaattcctca acaacttcat cggccaactg acgccgttct tcttctattc actgggcggc
      481 tacctcgtca tacagggcga cctatcattt ggcgcgctgg tggcgatcct cgccgcctac
      541 aaggacctgg cgtcgccctg gaaggaattg ctcgacttct accagatcaa ggagaactcg
      601 cggatcacct acgagcagat cgtcgagcag ttccagccct ccggcatgtc cgacgctcgg
      661 ttgttgctcg aggaaccggc cgtggtggcg ccgctgaccg gcgaactggc cgtggccaac
      721 ctgtcgctct ccgaggacga caagagccgc atcgtcgatg cggtgagctt cacggtcagg
      781 cttgacgagc atgtcgcggt gatcggccag agcggcagcg gcaagagcga actcgccttg
      841 atgctggcgc ggttggtgcg gccgagcagc gggcgcatca cgatcggcgg cctcgacctc
      901 gccgaattgc cggtcgcggt ggtcggccgc cgcatcggct atgtcggcgc catgccctac
      961 ctgttcgctg ggaccttgcg cgacaaccta ctgctcggct tgcagcatcg cccgctgcgg
     1021 ccggccgaat atgacgagga cgaggcccgc aagcgcgccc ggcagctctt cgaggcgcaa
     1081 cgttcgggca atatcgattt cgacctgcac gccgactgga tcgattacga gagcgccggg
     1141 gtcagcgacc gcgacgggct gatgcaccgc atttccgaag tcctggcgcg gctcgatttc
     1201 gaggaggatg tctacggcct tggcctgcgc ggtcggctcg acccggcgtc gcagcccgag
     1261 ctggccgagc gcctcttgga ggcgcgcaaa gcattggccg cccggctggt tagcgacgac
     1321 atcaccaagc tggtcgagac ctacgacctc gcgcgctaca actcgaatgc ctcggtggcg
     1381 gagaatgtgc tgttcggcac cccgatcggc ccggctttcg acttcgaggc gctcgccgac
     1441 aatgtccatg tgctgcgcgt cctcgaccag ctcgagctga ccggcgatct cgttgaggcc
     1501 ggccgcgagg tcgccgcgac gatgaccgag atgttcgccg gcctgccgcc cgagcatgaa
     1561 ttcttcgagc agttcagctt catcgacgcc aacgacctgc ccgaattcgc cgcgatcctc
     1621 tccgccgcgg aaaacggcgg cactgccgcg ctcgcgcagg agcagcggca caagctgttg
     1681 tccctgccgt tcaagctggt cgcggcacgc catcgcctcg gcgtgctcga cgagcgcatg
     1741 cagcagcggc tattggaagc gcgccgcgtt ttccgcgaga cactccctga ggaagctcgc
     1801 gaccagatcg agtttttcga ccccgaccgc tacaacgccg ccgcctcgct gcaggacaac
     1861 atcctgttcg gcaagatcgt caccggccag gccgaggccg ggacgcgcat cacgcgcctg
     1921 gtgcgcgagc tgctggacga gctcgggctg cggccgctgg tggtggagat cggcctcgac
     1981 taccaggtcg gtgtcggcgg cgcgcggctg cacgcggccg accggcagaa gaccgccatc
     2041 gtgcgcgcgc tcctcaagcg gccggtgttc atcgtgctcg accacgccgc ggcggtgctc
     2101 gatcccg
//
LOCUS       DASPXW010000164         2097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1058729, whole genome shotgun sequence.
ACCESSION   DASPXW010000164 DASPXW010000000
VERSION     DASPXW010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1058729"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VEL04_03870"
     CDS             complement(<1..211)
                     /locus_tag="VEL04_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47534.1"
                     /translation="MKALIRRYAERIDAATLRERVLIFLGLTLALVFIANAALLEPVR
                     AKQKSLAAEIALRQKELQTVQSQLER"
     gene            complement(208..885)
                     /locus_tag="VEL04_03875"
     CDS             complement(208..885)
                     /locus_tag="VEL04_03875"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HYT47535.1"
                     /translation="MSQQINLYNPIFLKQEKHFSARTMVQALGVIALGLIAVYAYALI
                     QSRDGERTAQQYRQQVVAQREQLLKLGAQLSSQGQSKALQAEIARLDAEVKSSQATLD
                     ALGTTELGNTAGFSDFLAAFGRQALAGVWLTGISIGESGNELVVHGRALRPELVPAYL
                     RALNKEPMMRGRRVTEMRLAAKSAPPAAGGAAGKARPAGPERFVEFTVTAPLRIAEAP
                     LAKGAAP"
     gene            complement(893..1837)
                     /locus_tag="VEL04_03880"
     CDS             complement(893..1837)
                     /locus_tag="VEL04_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="agglutinin biogenesis protein MshI"
                     /protein_id="HYT47536.1"
                     /translation="MRGLFARRRTREPGWLAVSMQPGALTFAHGVCGPAGKCEIRRCG
                     TRELGASFKDADRVAKDLGVDQYQCLTLLSPTDYQLLLVEAPNVPIAELKTAVRWRVK
                     DMLDYHLDDATIDVLDIPVDPASGGRGHSMYAVAARNDIIQGCIERFTAARIPLSVID
                     IAETAQRNIAALYEPPDRGVGLLYLGPAHALLTINFRGELYLARRIDVGIEQLLSAGE
                     AQRDEAMNRVLLELQRSFDHLDRQFPFVTMAKLLLGPEPRDTGLAAYLSANLDLAVEP
                     ASLSDVVSFEPGAPPEGEGAWRLFHVLGAALRNEVKTL"
BASE COUNT          358 a          662 c          713 g          364 t
ORIGIN      
        1 tgcgctcgag ctgggactgg acggtctgca gctccttctg gcgcagcgcg atttcggcgg
       61 cgaggctctt ctgctttgcg cggaccggct cgagcagcgc ggcgttggcg atgaacacca
      121 gggcaagcgt cagcccgagg aaaatcagca cgcgctcgcg cagcgtcgcg gcgtcgatgc
      181 gctcggcgta gcgccggatg agcgccttca cggcgcagct cccttggcga gcggggcttc
      241 ggcgatgcgc agtggagccg tcacagtaaa ttcgacaaaa cgctcgggcc ccgcggggcg
      301 tgcctttcca gcggcgccac ccgcggcggg cggtgcgctc ttcgcggcga gcctcatctc
      361 ggtgacgcgg cgcccgcgca tcatcggctc cttgttcaac gcacgcaggt acgcgggcac
      421 gagctcgggc cgcagggcac ggccgtgcac caccagctcg tttccggatt ccccgatact
      481 gatgccggtg agccacaccc cggccagcgc ctgcctgccg aaggcggcga gaaaatcgga
      541 aaacccggca gtgttgccga gctccgtggt acccaatgcg tcgagcgtcg cctggctcga
      601 tttgacctcg gcgtcgagcc gggcgatctc ggcctggagc gccttgctct gtccttggga
      661 ggaaagctgc gcgccaagct tgaggagctg ctcgcgctgc gccacgacct gctgccggta
      721 ttgctgcgcg gtgcgttcgc cgtcgcgcga ctggatcaag gcataggcgt acaccgcgat
      781 cagtccgagc gcgatcacgc caagcgcctg caccatggtg cgcgcggaaa aatgcttttc
      841 ctgcttgagg aagatcgggt tatagaggtt gatctgctgg ctcaccgcgg cctcagagcg
      901 tctttacttc gttgcgcagt gcggcgccga gaacgtgaaa cagccgccag gcgccctcgc
      961 cttcgggcgg tgcgcccggt tcgaagctga ccacgtcgga aaggcttgcg ggctccaccg
     1021 ccaggtcgag gttcgccgac aggtaggccg ccagcccggt atcccgcggc tcgggcccca
     1081 gcagcagctt agccatcgtg acaaagggaa actggcggtc gaggtgatcg aagctgcgtt
     1141 gcagctcgag gaggacgcgg ttcatcgcct cgtcccgctg cgcctcgccc gcgctgagaa
     1201 gctgctcgat gccgacgtcg atgcgccggg cgagatagag ctcgccgcgg aaattgatgg
     1261 tcagcagcgc gtgcgccggg ccgaggtaga gcaggccgac gccgcggtca ggcggttcat
     1321 agagcgccgc gatgttgcgc tgcgcggtct cggcgatgtc gatcaccgaa agcgggatgc
     1381 gcgcagccgt gaagcgctcg atacagccct ggatgatgtc attgcgggcg gcgacggcgt
     1441 acatcgagtg gccgcgtccg ccgctagccg ggtcgaccgg aatatcgagc acgtcgatgg
     1501 tggcgtcgtc gaggtgatag tcgagcatgt ccttcacgcg ccagcgcacc gcagtcttca
     1561 gctcggcgat cggcacattg ggcgcctcga caagcagcag ttgatagtcg gtgggcgaca
     1621 gcagcgtcaa gcattgatac tggtcgacgc ccaggtcctt cgcgacgcgg tccgcatcct
     1681 tgaacgaagc gccgagctcg cgcgtgccgc aacgcctgat ttcgcacttt cccgccggcc
     1741 cgcacacgcc gtgagcgaac gtcagcgctc cgggctgcat ggacactgcc agccagcccg
     1801 gctcgcgagt gcggcgccgt gcgaagagtc ctctcattac gctagccgag ttatctcgga
     1861 tttgacttca aattatagca ataaccaatt gttgtcaaat gggtttcttt gtcagaagca
     1921 gttcgaaaag caaggccccc gccgccggtc tgttttggcg gtcggacaaa gagctagagg
     1981 gttacttggt aagggtgagc tggagctggc gctcaacata cgtcgggccc aggctgccgc
     2041 aggcgatgct gaggttgcag ccagtgacgg taaggcgata gacaacgact gtgacgc
//
LOCUS       DASPXW010000165         2749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1058912, whole genome shotgun sequence.
ACCESSION   DASPXW010000165 DASPXW010000000
VERSION     DASPXW010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1058912"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="VEL04_03885"
     CDS             <1..518
                     /locus_tag="VEL04_03885"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HYT47537.1"
                     /translation="HAVRACVSASRRERVNRMMKRLDTTNVEEAGRIVRQNDEAAQAV
                     MRRHFHIDVRDINEYDIGFNTDRMSVAQCTDEILKMVKSQTFAETDESRAKLRDVAIG
                     HHVRAALRTHEATAHCWVRVSAKAGRVTLEGNVESREQVETCAGIASRIKGVAELDNC
                     LRAAPRPRGAR"
     gene            529..1305
                     /locus_tag="VEL04_03890"
     CDS             529..1305
                     /locus_tag="VEL04_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYT47538.1"
                     /translation="MDLGLKGKNVLITGGSKGIGFACAQAFRSEGARVAIVSRSQANL
                     DAAKKTLHECYTISADLTNAAAAAAMVERVEKEFGLLDILVSSAGAARRTNADELTPE
                     AWRAAMDAKYFSYVNVIDAVVKRMAARGRGAIVNVIGQGGKVPSPVHIAGGAANAALM
                     LATAGLANAYAARGVRVVGVNPGQTRTDRVAEGLVAEAKRAGISEEEALKRTVAKIPL
                     GRMAEPGEIADVVVFAASDRGRFLTGANITMDGGSSPTVV"
     gene            complement(1321..>2749)
                     /locus_tag="VEL04_03895"
     CDS             complement(1321..>2749)
                     /locus_tag="VEL04_03895"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HYT47539.1"
                     /translation="ARARVRPDGLRAGTGALQGKVNDPAAWSLAEAARALRQREVAPR
                     ELVEACFARIERWQPRINAFVSQEKNAAGDANGAIAGVPLAHKDMFYRAGRVSNCGSK
                     IRRGWKASETSAALEGLDRAGALQIGTLNMSEFAYGPTGHNEHWGDCRNPWHPDHITG
                     GSSSGSGAAVAARLAFGALGSDTGGSVRLPAAACGVTGLKTTWGRVSRHGAMPLSHSL
                     DTIGPLARSAEDCALLLAAIAGHDPRDPVSSREPVPDYSKNLVVKGFRVALSTAWIER
                     HTQPQVAAAVLGAASLLRSAGVKLIEAPAPDFDALSAHCLVVMQAEASAQHAKWMRER
                     PADYSSAVRARLEAGFAIPAAAYLESLRLRAAWLERFCSTTLAGADLYLLPAINVPVP
                     TREQTGPRGGGEMPKLLGEVTRLTRWVNYLGVPALVVPCGFDSRGLPVAIQLVGRPFA
                     EATLLAAGHAYQRETDWHQRVPAER"
BASE COUNT          460 a          937 c          983 g          369 t
ORIGIN      
        1 cgcacgcggt gcgcgcgtgc gtctctgcct cgcgccgcga acgcgtaaat cgcatgatga
       61 agcgcctcga caccaccaac gtggaagagg ccggacgcat cgtgcgccag aacgacgagg
      121 cggcgcaggc ggtgatgcgc cgccacttcc acatcgacgt gcgcgacatc aacgaatacg
      181 acatcggctt caataccgac cgcatgagcg tcgcgcagtg caccgacgag atcctgaaga
      241 tggtgaagtc gcaaacgttc gccgagaccg acgagtcgcg cgccaagctg cgggacgttg
      301 cgatcggcca ccacgtgcgc gccgcgctgc gcacccatga ggcgaccgcg cactgctggg
      361 tgcgggtgtc cgcgaaagcg ggccgcgtca cgctcgaggg aaacgtcgag agcagagagc
      421 aggtcgagac ctgtgccggg atcgcctcgc gcatcaaggg tgtggccgag ctggacaact
      481 gcctgcgcgc ggcgccgcgg ccgcgtggcg cgcgctagcg gcgccggcat ggatctgggc
      541 ctgaagggca agaacgtgct catcaccggc ggcagcaagg gcatcggctt cgcctgcgcg
      601 caggcgtttc gcagcgaagg cgcgcgcgtc gccatcgtgt cgcgttccca ggccaacctc
      661 gatgcagcga agaagaccct gcatgagtgc tacaccatct ccgccgacct gacgaacgcc
      721 gcggcggccg cggcgatggt cgagcgggtg gagaaggagt tcgggctgct cgacatcctg
      781 gtaagcagcg ccggcgccgc gcggcgcacc aacgccgacg agctcacccc ggaggcgtgg
      841 cgcgcggcaa tggacgcgaa gtacttcagc tacgtcaacg tgatcgacgc agtggtgaag
      901 cgcatggcgg cgcgcggccg cggcgcgatc gtcaatgtga tcggccaggg cggcaaagtg
      961 ccctcgccgg ttcacatcgc gggcggcgcg gcaaacgcgg cgctgatgct cgcgaccgcc
     1021 ggcctggcga acgcctacgc cgcgcgcggc gtgcgggtgg tcggggtcaa ccccgggcag
     1081 accaggaccg accgcgtcgc cgaggggctc gtcgccgaag cgaagcgcgc cggcatctcc
     1141 gaggaggagg cgctcaagcg cacggtcgcg aaaatacctc tcggccgcat ggccgagccc
     1201 ggggagatcg ccgatgtggt ggtgttcgcg gcttcggacc gcggccgatt cctcaccggc
     1261 gccaacatca ccatggacgg cggatcgtcg ccgacggtgg tttagtagtg cgaccagcgg
     1321 ctagcgctct gccggcacgc gctgatgcca gtcggtttcg cgctggtacg cgtgtcccgc
     1381 cgcgagcagc gtagcttccg cgaacgggcg gccgaccagc tggatcgcga ccggcaggcc
     1441 gcgcgagtcg aagccgcagg gcacgacgag cgcgggcacg ccgaggtaat tgacccagcg
     1501 cgtaaggcgc gtgacctcgc ccagcagctt cggcatctcg ccgccgccgc gcggcccggt
     1561 ctgctcgcgc gtggggacgg gcacattgat cgccggcagc aggtataagt cggcgcccgc
     1621 aagcgtcgtc gagcagaagc gctcgagcca cgccgcgcgc agccgcaggc tttcgagata
     1681 cgccgctgcg ggaatcgcga agcccgcctc gaggcgcgcg cgcaccgcgg aggaataatc
     1741 ggccgggcgc tcgcgcatcc actttgcgtg ctgggcggag gcctcggcct gcatcacgac
     1801 gaggcaatgc gccgacaacg catcgaaatc gggcgccggc gcctcgatca gcttgacgcc
     1861 ggccgagcgc aggaggctgg ccgcgccgag gaccgccgcg gcgacttgcg gttgcgtatg
     1921 ccgttcgatc caggcggtgg agagcgcaac gcgaaatcct ttcaccacca gattcttgga
     1981 gtaatcgggc actggttcac gcgagctcac gggatcgcgc ggatcgtggc cggcgatcgc
     2041 ggcgaggagc agcgcgcaat cctctgcgct gcgggcgagc ggcccgatgg tgtcgagcga
     2101 gtgggaaagc ggcatcgcgc cgtggcgaga caccctgccc cacgtggtct tcaggccggt
     2161 caccccgcag gccgccgcag gaagccgcac cgagccgccg gtatcggagc cgagcgcgcc
     2221 gaacgcgagc cgcgccgcca ctgccgcgcc cgatccgctg gaagagccgc cggtgatgtg
     2281 gtccggatgc cacggattgc ggcaatcgcc ccagtgctcg ttgtgcccgg tcggcccata
     2341 ggcgaactcg gacatgttga gcgtgccgat ctgcagcgcc ccggcccggt ccagcccctc
     2401 gagagctgcg gaagtctcgg acgccttcca gccgcgccgg atcttcgagc cgcaattcga
     2461 aacgcggccg gcgcggtaga acatgtcctt gtgcgcgaga ggaacgcctg caatggcccc
     2521 gtttgcatcg ccagccgcat ttttttcctg ggacacgaag gcgttgatgc gaggctgcca
     2581 gcgctctatg cgcgcgaagc aggcctcgac aagctcgcgc ggcgcaacct cgcgctggcg
     2641 cagcgcgcgc gcggcttcag cgagagacca agccgccgga tcgttcactt tgccttgcag
     2701 cgctccagtg ccagcgcgta gcccgtcggg tcggactcga gctcgagcg
//
LOCUS       DASPXW010000166         6682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_300195, whole genome shotgun sequence.
ACCESSION   DASPXW010000166 DASPXW010000000
VERSION     DASPXW010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6682
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300195"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="VEL04_03900"
     CDS             complement(<1..867)
                     /locus_tag="VEL04_03900"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47540.1"
                     /translation="MPDFREQYEKASQLGKLAFVERPYIGSPGAPAQAETNASQIWGH
                     INAGYLFAYEYTRWWKESRALRNAAVLGDWSWLNKTRVRGSDAARFMNYATVKDLAKQ
                     QPGQIVYTPMVDAGGKVAIEGLTLKLGDNDYLFTQSGALKWFEFLRNTTRMKVELEDV
                     TPDYTCYALQGPRSTDVLEALTGESWKDLRFSRFRKTKILETETLVARQGVTGEIGYE
                     FLMRTDSGRAHELWRRLRDTGRDFGLRELGFKAQMVGHTETGIATVIRDFLPARGKPE
                     AMRKMLRYWMSAE"
     gene            879..2390
                     /locus_tag="VEL04_03905"
     CDS             879..2390
                     /locus_tag="VEL04_03905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HYT47541.1"
                     /translation="MRAKRSIIDYLAEHASRSLERVAFHYKDRTVSFRELEESAARCR
                     GALAAQGVKPGDRVALVMSDCPEMAIAMLGVMGMGAIVVPCSTMLKPAELEYMFKDSG
                     AKLVIVTPEHAENAKAAHAPKIIVAPAEFNELLEKSKPAALGDFDRDTPCLILYTSGS
                     TGSPKGAVHLHDHLPETFERVARKVYELGPDDRIFSSSRLFFAYGLGNSFSFPIGAGC
                     TAILCSERPTPQVIADIFARYRPTVFFGVPAVFRGLIEYARQGNALDTRSLKFCVSAG
                     EVLPVATWKEWKEVSGVEIIETIGTTELLHAFIHNFRDRNRPGSSGVPLEGYQIKLTD
                     ENGKAIEGAGHGNLHVKGRSAIPYYLNQPAKTSELIRDGWVKTGDVYRRDEEGFYWFE
                     GRSDDLFKCSGMWVSPGEVEEAVIRHPAVLEAAVVAEADDKGATIAAAYVALRPGHSA
                     GDALSKEIMEEAAKSLPRFKRPQRIHFMEALPRTATGKVQRFKLRQAQSRPGP"
     gene            complement(2406..3488)
                     /locus_tag="VEL04_03910"
     CDS             complement(2406..3488)
                     /locus_tag="VEL04_03910"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYT47542.1"
                     /translation="MEFGYFTLSDNHYPNNPRSANQFVLEIREQAILADRLGMHSCWI
                     GEHHFDSLGVNSRPDLLLSNIAQVTKHVRLAPAVTVLPLHHPVHVAEAWATLDLLSGG
                     RVDFATGRGYDRKEYAPFGTDFMASAEIFEEGIDIVLKAWNSAGPWSHDGKYYRIPPM
                     SITPKPVQKPIPFYVASFSKTSLDMAAKRGLNIIYAPFAAGMVFGGLDKAVDSYREAC
                     VKAGKKPGRAMCSYFIFIADDAKAEDYGRQTQMDYFNHCVLQAFPQKPEDAPPTMQYF
                     MKIVEILKSMKKEDLSERSILLGRPEKIVETLKKVEKSGVEEVILYFNVGNKPHNLVK
                     EQMHRFMDEIAPHFAGKHLERTKIAV"
     gene            3527..4177
                     /locus_tag="VEL04_03915"
     CDS             3527..4177
                     /locus_tag="VEL04_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT47543.1"
                     /translation="MLRLSGVTKRFGDRAVLADISLAVAAGEYVAVIGESGIGKSTLL
                     NLIAGLEPVDAGSIFFGEDEITGLGDDALTLLRREKFGFVFQAFHVLPHLSVEQNVGL
                     PLLLRKIENKEKVGRLIAAVGLAGREQSMPRELSGGELQRVAIARALVGDPQLVLADE
                     PTGNLDPENARQVLALFKAQVKERGAAAILATHSEQAAAGCDRRFRLSAQGLTQSD"
     gene            complement(4161..5078)
                     /locus_tag="VEL04_03920"
     CDS             complement(4161..5078)
                     /locus_tag="VEL04_03920"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HYT47544.1"
                     /translation="MRVLFRFDASEALASKAAALSGDGTEILTAPESDDAAYDRLLPG
                     IDVLWHVLRPVNEDQINRASSLKLIQKIGSGLNTIDVEAAKRRGIAVCNLPGTNSRAV
                     AEMALLLMLACLRRLSQLNSYVRTENGWQEAWQLQDHFGELGGRTVGLIGYGEVPKIL
                     KPILEAMGATVLYWSRSQRNSEFKTLVATSDIVSLHLPLTSQTEKIIDARAMKAGAIL
                     VNTARGGLVDEECLIQSLKTGHLAAAGLDVFPREPPPADHPLLKLPNVVCAPHLAWLT
                     QETLERSLGAALENVKRLEAGRPLLNRIV"
     gene            complement(5107..6360)
                     /locus_tag="VEL04_03925"
     CDS             complement(5107..6360)
                     /locus_tag="VEL04_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HYT47545.1"
                     /translation="MAKIIDIKGRRVWDSRGRATVEAEVIVGRGTTRLALGRAIAPAG
                     ASLGSGEAKSVDVVKAVHQINTRVRDGLRALSVNDQEALDRRLIELDGTPDRSRLGAN
                     AMVAVSLACAQADAAAQKIPLWKKLSGERRVALPVPQIQIFGGGAHARGRVDVQDYMI
                     ICTGAGSFAEALDWTSEVYAAAGARLAKRGALQGVADEGGYWPSFKSNEEGLAELVGA
                     IADARLEPGVDVCIALDIAATQLYRAGQYYFSLERKSLSAEQLHAMLLRWIEAYPIVS
                     IEDPFAEHDTEAMQAFTQAIGEKIQVVGDDYFVTDARRVKAAEGACNSVLLKPNQVGT
                     VTETFACWDAARAAGYRAIMSARSGETEDVSIVHLAVGWGVPQLKVGSFSRSERMAKW
                     NEALRIEEALGGKALPFPARKLFKK"
     gene            complement(6339..>6682)
                     /locus_tag="VEL04_03930"
     CDS             complement(6339..>6682)
                     /locus_tag="VEL04_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HYT47546.1"
                     /translation="WYGDPAFDLAFCLNHMLLKSVWVPQARDGFLHCFDALVHPYLSR
                     VTWEVPAALEERAATLLPGLLLARIDGKSPVEYLDEAARARVRNAARRLIFLKKKTLG
                     EVKSAWQKSST"
BASE COUNT         1164 a         2163 c         2120 g         1235 t
ORIGIN      
        1 ttccgccgac atccagtagc gcagcatctt gcgcattgcc tccggcttgc cgcgcgccgg
       61 gagaaaatcg cggatcacgg tggcaatgcc ggtctcggta tggccgacca tctgcgcctt
      121 gaagccgagc tcgcgcagcc cgaaatcccg gccggtgtcg cggagcctgc gccacagctc
      181 gtgcgcgcgg ccgctgtcgg tgcgcatcag gaactcatag ccgatctctc cggtgacgcc
      241 ctggcgcgcc accagagttt ccgtctcgag aattttcgtt tttcgaaacc ttgagaaccg
      301 caaatctttc cagctctcgc cggtgagagc ctccaatacg tcggtagagc gcgggccctg
      361 cagcgcgtag caggtgtagt ccggcgtcac atcctcgagc tcgaccttca tgcgcgtcgt
      421 attgcgcagg aactcgaacc atttcaatgc gcccgactgc gtgaagaggt agtcgttgtc
      481 gccgagcttg agcgtcagcc cttcgatcgc caccttgccg ccggcatcga ccatcggggt
      541 gtagacaatc tgcccgggct gctgcttcgc aagatccttc accgtcgcgt agttcatgaa
      601 gcgcgccgcg tcggacccgc gcacccgggt cttgttcagc cagctccagt cgccgagcac
      661 cgccgcattg cgcagcgccc gggattcctt ccaccagcgc gtgtactcgt acgcgaacag
      721 gtaacccgcg ttgatgtggc cccagatctg gctggcgttg gtctccgcct gcgccggggc
      781 ccccggcgag ccgatatagg gccgttcgac gaacgcgagc ttgccaagct ggctcgcctt
      841 ctcgtactgt tcccggaaat caggcatctt ccactcccat gagggccaag cgcagcatta
      901 tcgattacct cgccgagcac gcgagccgca gcctcgagcg ggttgctttt cactacaagg
      961 acaggacagt cagcttccgc gagctcgagg agagcgccgc acgctgccgc ggagcgctgg
     1021 cggcgcaagg cgtgaagcct ggcgaccgag tggcgctggt gatgagcgat tgccccgaga
     1081 tggcgatcgc catgctcggc gtcatgggca tgggcgcgat cgtggtgcct tgcagcacca
     1141 tgctcaagcc ggccgagctc gagtacatgt tcaaggacag cggcgccaag ctcgtgatcg
     1201 tgactccgga gcacgccgaa aacgccaagg cagcccacgc gccgaagatc atcgtcgcac
     1261 cggcggaatt caacgagctg ctcgaaaaat caaaaccagc cgcgctgggc gatttcgacc
     1321 gcgacacacc ctgcctgatt ctttatacgt cgggcagcac cggctcgccg aagggcgcgg
     1381 tccacctcca tgaccatctg ccggaaacct tcgagcgcgt ggcgcgcaag gtctacgagc
     1441 tgggtcccga tgaccggatc ttctcttcgt cgcgcctgtt cttcgcctac ggcctgggga
     1501 acagcttctc gttcccgatc ggcgccggct gcacggcgat cctgtgcagc gaacgcccga
     1561 cgccccaggt cattgccgac atcttcgcgc gctaccgccc gacggtgttc ttcggcgtgc
     1621 ccgctgtatt ccgcggcctt atcgaatacg cgcgccaggg aaatgcgctc gacaccaggt
     1681 cgctcaagtt ctgcgtctcg gcgggcgagg tgctgccggt ggctacctgg aaggagtgga
     1741 aggaagtgag cggcgtcgaa atcatcgaga ccatcggcac cacggaactg ctgcatgcct
     1801 tcatccacaa tttcagggac cgcaaccggc cgggcagctc gggcgtgccg ctcgaaggct
     1861 accagatcaa gcttaccgac gagaacggca aggccatcga aggtgccggg cacggcaatc
     1921 tgcatgtcaa gggccggtcg gcgattccct actacctgaa ccagcccgct aaaacatccg
     1981 agctgatccg cgacggctgg gtgaagaccg gcgacgtcta tcgccgcgac gaggagggct
     2041 tctactggtt cgaggggcgc agcgacgatc ttttcaagtg ctcgggcatg tgggtctcgc
     2101 cgggcgaggt ggaggaggcg gtcatcaggc acccggcggt gctggaagcg gccgtcgtcg
     2161 ccgaagccga cgacaagggc gccaccatcg ccgctgctta cgtagcgctg cgtcccggcc
     2221 actcggccgg ggatgctctt tcaaaagaaa tcatggagga agcggcaaag agcctgccgc
     2281 gcttcaagcg cccgcagcgc atccacttca tggaagcgct gccgcgcacc gctaccggca
     2341 aggtgcagcg cttcaagctg cgccaggcgc aatccaggcc gggtccctga agatccggat
     2401 tcatttcaga cggctatttt cgttcgctcc aggtgcttgc cggcgaaatg gggcgcgatc
     2461 tcgtccatga agcggtgcat ctgctccttg acgaggttgt gcggcttgtt gccgacgttg
     2521 aagtacagga tcacctcctc gacgccgctt ttctccactt tcttcagcgt ctcgacgatc
     2581 ttctccggcc ggccgagcag gatcgagcgc tcggaaagat cctccttctt catgctcttc
     2641 aggatctcga cgatcttcat gaagtactgc atggtcggcg gcgcgtcctc gggcttctgc
     2701 ggaaaggcct gcagcacgca gtgattgaag tagtccatct gcgtctgccg gccgtagtcc
     2761 tcggccttgg cgtcgtcggc aatgaagatg aagtagctgc acatggcgcg cccgggcttc
     2821 ttgcccgctt tgacgcatgc ctcgcgatag ctgtcgaccg ccttgtcgag gccgccgaac
     2881 accatgccgg cggcgaacgg cgcgtagatg atgttgagcc cgcgcttcgc cgccatgtcg
     2941 aggctcgtct tggagaacga ggcgacgtag aacgggatcg gcttctgcac cggcttcggc
     3001 gtgatcgaca tcggcgggat cctgtagtac ttgccgtcgt gcgaccaggg acccgccgag
     3061 ttccaggcct tgagcacgat gtcgatgcct tcctcgaaga tctcggccga agccatgaag
     3121 tcggtgccga agggcgcgta ttccttgcgg tcgtagccgc gcccggtggc gaaatcgacg
     3181 cgcccgccgg aaagcagatc gagcgtcgcc caggcttcgg cgacgtgcac cgggtggtgc
     3241 agcggcagca ccgtcacggc aggggcgagc cgcacgtgct ttgtgacttg cgcaatgttg
     3301 gataaaagca gatcggggcg cgagttcacc cccagcgaat cgaagtggtg ctcgccgatc
     3361 cagcacgagt gcatgccgag ccggtcggcg aggatcgcct gctcgcggat ctcgagcacg
     3421 aactgattgg ccgagcgcgg attgttcggg tagtggttat cgctgagggt gaagtagccg
     3481 aattccatga tcctcctccc gtgctacagc atagtacgat tccaaggtgc tgaggctctc
     3541 cggggtcacc aagcgtttcg gcgaccgcgc tgtcctggcc gatatctcgc tcgccgtcgc
     3601 cgccggcgaa tacgtcgccg tgatcggcga gtccggcatc ggcaaatcga cgctgctcaa
     3661 cctcatcgcc gggctcgagc cggtcgacgc gggaagcatc ttcttcggcg aagacgagat
     3721 caccggcctc ggcgacgatg cgctgaccct gctgcggcgc gagaaattcg gcttcgtgtt
     3781 ccaggctttc cacgtcctgc cgcacctcag cgtcgagcag aacgtcggtc tgccgttgct
     3841 tctgcggaaa atcgaaaata aggaaaaagt cggccgtctg attgctgcag tcgggctcgc
     3901 cgggcgcgaa cagagcatgc cgagggagct ctcgggcggc gagctgcagc gcgtcgccat
     3961 cgcccgcgcg ctggtcggcg acccgcagct ggtgctcgcc gatgagccca cggggaacct
     4021 cgatcctgaa aatgcgcggc aggtcctggc gctttttaag gctcaggtaa aggaacgagg
     4081 cgcggcagcg atcctcgcca cgcactccga gcaggcggcc gcgggctgcg accggcgctt
     4141 ccggcttagc gcccaggggc ttacacaatc cgattgagga gcggcctgcc ggcctcgagg
     4201 cgtttcacgt tttcgagcgc cgcgccgagg ctgcgctcga gcgtctcctg agtaagccac
     4261 gccaggtgcg gcgcgcacac gacgttcggc agcttcagca gagggtgatc ggccggcggc
     4321 ggctcgcgcg ggaagacatc gagcccggcg gccgccagat gtcccgtctt caggctttga
     4381 atcaagcact cttcgtcgac caggccgccg cgggcggtat tcaccaggat cgcgccggct
     4441 ttcatagctc tcgcatcgat gattttttca gtttgcgaag tgagcggcag atggagcgag
     4501 acgatatcgg acgtggcgac cagcgttttg aactctgaat tgcgctgcga gcgtgaccag
     4561 tacagcaccg ttgcgcccat cgcttccagg atcggcttga gtatcttcgg cacttcgccg
     4621 tagccgatca ggcccacggt acggccgcca agctcgccga agtgatcctg taattgccag
     4681 gcttcttgcc agccattctc ggtccttaca taggaattca actgagaaag acgccgcaga
     4741 catgcgagca tcaggagcag cgccatctcg gctaccgcgc ggctgttggt gccagggagg
     4801 ttgcagaccg cgatgccgcg gcgcttcgcc gcctcgacgt ctatggtgtt gaggccgctg
     4861 ccgattttct ggatcagctt caagctggag gcgcggttga tctgatcctc gttcacgggc
     4921 ctcagcacgt gccaaagcac atcgattccc ggaagaagac ggtcatacgc ggcatcgtcc
     4981 gattcgggag ccgtgagaat ctcggttccg tcaccggaca atgcggctgc cttgctcgct
     5041 agcgcttcgc ttgcatcgaa ccgaaacagg actctcacgg cgtaatgaga aaaatggggt
     5101 ctgtccctat tttttgaaca gctttctcgc ggggaagggc aaggccttgc cgccgagcgc
     5161 ctcctcgatg cgcagcgcct cgttccattt cgccatgcgc tcggagcgcg agaagctgcc
     5221 gaccttgagt tgcggcacgc cccagccgac cgcgaggtgg acgatcgaca cgtcctcggt
     5281 ctcgccggag cgcgccgaca tgatcgcccg atagccggct gcgcgtgccg catcccaaca
     5341 ggcgaaggtc tcggtcacgg tgccgacctg gttgggcttg aggagcacgc tgttgcacgc
     5401 tccctcggcg gccttgaccc gtcttgcgtc ggtaacgaaa taatcgtcgc cgacgacctg
     5461 aatcttctcg ccaatcgctt gcgtgaacgc ctgcattgcc tcggtgtcat gctccgcgaa
     5521 agggtcctcg atcgagacga tggggtaggc ctcgatccag cgcagcagca tggcgtgaag
     5581 ctgctccgcc gaaaggcttt ttctttcaag agaaaagtaa tactggccgg cgcggtagag
     5641 ctgggtcgcc gcgatgtcga gcgcgatgca gacgtcgacc ccgggctcga ggcgcgcatc
     5701 ggcgatggcg ccgacgagct ccgccagccc ttcctcgttc gacttgaagc ttggccagta
     5761 gccgccctcg tcggcgacgc cctgcagcgc gccgcgcttc gcgagcctcg cccccgcggc
     5821 ggcatagacc tcggaggtcc agtcgagcgc ctcggcgaag ctgcctgcgc cggtgcagat
     5881 gatcatgtag tcctgcacgt cgacgcggcc ccgcgcatgg gcgccgccgc cgaagatctg
     5941 gatctgcggc accgggagcg cgacgcgccg ttcccccgaa agctttttcc acaagggaat
     6001 tttttgtgcg gcggcgtcag cctgcgcgca ggcgagcgag acggcgacca tggcgttggc
     6061 gccgagccgg ctccggtccg gggtgccgtc gagctctatc aggcgccggt cgagcgcttc
     6121 ctggtcattg acgctgagcg cacgcaggcc gtcgcgtacg cgcgtgttga tctgatgcac
     6181 cgccttgacg acgtcgacgc tcttcgcttc gcccgagccc agcgacgcac ccgccggcgc
     6241 gatggcgcgg cccagggcga gccgcgtggt gccgcggccg acgatcacct cggcctcgac
     6301 ggtggcacgg ccgcgcgagt cccacacgcg gcgacccttt atgtcgatga tttttgccat
     6361 gcgctcttca cttcgcccaa tgtttttttc ttgagaaaaa tcaaacgccg ggcagcgttg
     6421 cgtacccgcg ctctcgcagc ttcatcgaga tattccaccg gcgacttgcc atcgatgcgc
     6481 gcgagcagca gcccgggcag gagcgttgcc gctcgctctt ccaatgcagc gggcacctcc
     6541 caggtcacgc gtgagaggta tggatgcacc aatgcatcga aacagtgcaa aaacccgtcg
     6601 cgcgcctgag gcacccagac cgatttcagc agcatgtggt tgaggcagaa cgcgaggtcg
     6661 aacgcgggat cgccgtacca gg
//
LOCUS       DASPXW010000167         4925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1185275, whole genome shotgun sequence.
ACCESSION   DASPXW010000167 DASPXW010000000
VERSION     DASPXW010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4925
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185275"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="VEL04_03935"
     CDS             complement(<1..739)
                     /locus_tag="VEL04_03935"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HYT47547.1"
                     /translation="MSGMSFVPLSALELRDAVRGSKSYDPLRLDRILRVDEDLGLVEV
                     QASSAWTTLAKRLRPSDARAASARTTMPTVGESIARNAAGPDGRTAVTHVESLTLVTP
                     DGELRRASRYSNPQLFSLAVGGQGVFGALYSVTLRVDSLARALEETAPGETLQQAAGG
                     ARGRTLHLLVPAERLDVCITQVRAHCAEWRSAIESVELRRIRKESETYLRWACRDYTE
                     IRVQLARPGSIAASVRATQLARELLDIA"
     gene            complement(784..1062)
                     /locus_tag="VEL04_03940"
     CDS             complement(784..1062)
                     /locus_tag="VEL04_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HYT47548.1"
                     /translation="MVTRQIRVRGRVQGVGYRAALQDEALKLGITGWVRNRSDGSVEA
                     LVQGSPPAVEAIVSWAARGPAGARVDALEVAGLDDAPACAQFELRATL"
     gene            1111..1503
                     /locus_tag="VEL04_03945"
     CDS             1111..1503
                     /locus_tag="VEL04_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47549.1"
                     /translation="MRIGLLLVAGLALIGFLVVAVVLPQMAGAEARDAAQALIAGAEP
                     ARQQISATAEKSARLAGAGKGIKIAPKTDPKHGDMKWIVGDDGSVRGWNEKNALEIAL
                     TPTLQGGKVSWNCKGYPVNAMPASCGGR"
     gene            complement(1469..1999)
                     /locus_tag="VEL04_03950"
     CDS             complement(1469..1999)
                     /locus_tag="VEL04_03950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HYT47550.1"
                     /translation="MYQLLKQIAKTGIVSERAPGPDESMRELEQRLERVVLEHFGRSL
                     AIRHVDAGSCNGCELEIHACNSPHYNLERLGIRFVASPRHADLLLVTGPVSRNMETAL
                     RRTYDATPEPKLVVAIGDCGCDGGIFGESYASRGAVANVIPVDVAVPGCPPPPTAIIQ
                     GILTAVTARRSLPASR"
     gene            complement(2002..3552)
                     /locus_tag="VEL04_03955"
     CDS             complement(2002..3552)
                     /locus_tag="VEL04_03955"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HYT47551.1"
                     /translation="MTHPFTDLAIIDAQRLPGPLPAWHASIERGALRGIAEKARALKA
                     RLVALWGSDETPRGAGYALHVTLALRPGLVWLTVPLEREHPRYSGIADIFPAANRMQR
                     AAYDLVGVHAHENPDHRKWLRHGAWPGGEFPLRKDFDAAAGFAPGEDRYPFVRVEGEG
                     VHEIPVGPVHAGTIEPGHFRFSIVGEKVLRLEERLGYKHKGIEKRFESLGPEDGARLA
                     GRVSGDSTVAYAWAYAMALEAASGVEPPPRAVALRGIALELERVANHLGDLGYLGNDV
                     ALSFGFSQFWRLKEELLRLHHELFGHRYLMDFIVPGGVARDLLAPEILVEKLGEIGAE
                     VATLRAIYDEHAGAQDRFILTGQVTPELAERLGLTGLAGRASGQAHDLRCDYPVPPYD
                     ALAVRIGEHIRGDVAARISVRFEELAESLRLIEALAERLCGGELRAALPAMQPGRRGC
                     GWIEGWRGEVFIALDMGNGGRPHRVHAHDPSWQNWPVLERAVLGNIVPDFPLINKSFN
                     LSYSGHDL"
     gene            complement(3549..>4925)
                     /locus_tag="VEL04_03960"
     CDS             complement(3549..>4925)
                     /locus_tag="VEL04_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HYT47552.1"
                     /translation="WAAEASAFMSLGTLAAATLLTARVIAEGPMTALNEQVFVDPFNV
                     FLVALTALVAFTTALFSRPYMRTEELHKRVNSSRLRLYHSMYQLFTFMMLLTLLSNNV
                     GMLWVAMEGATLSTVLLVSLYRTPASLEAAWKYFILCSVGIAQALFGTILLYFAAEKV
                     LGPGGDALLWTHLHEVRGELEPTVLSLSFVFLLVGYGTKVGLVPLHNWLPDAHAEGPT
                     PISAVLSGLLLNVALYAVVRGKVLVDGALERNFSGGLMMGFGLVSVVVAAFFLSRQKD
                     IKRMFAYSSIEHMGIMTFAFGMGGPVASFAGLLHMTVHSLTKSAIFFTVGHASQACGT
                     QDMASIRGLIQHSPTIGWGLALGTIAILGMPPFGVFTSEFLVLTTAMREHPWATPFLL
                     ISLGIAFAAIFGKVQPMIFGETSVQRLPHPPALVPVFVHLAIVLMLGLYIPPYLVDWY
                     RQAAAFIG"
BASE COUNT          885 a         1641 c         1680 g          719 t
ORIGIN      
        1 tcgcgatgtc gagcagctcg cgcgcgagct gcgtcgcgcg gacggaggcg gcgatcgagc
       61 cgggccgagc gagctgcacg cggatctcgg tgtagtcgcg gcatgcccag cgcagatagg
      121 tttcgctttc tttccgaatg cgccgcagct cgacgctctc gatcgcgctg cgccactccg
      181 cgcaatgcgc gcgaacctgg gtgatgcaga catcgaggcg ctcggcaggc acgagcaggt
      241 gcagcgtgcg cccgcgcgcg ccgccagcgg cctgctgcag cgtttcgccg ggcgcggttt
      301 cctcgagcgc gcgcgcaagc gagtccacgc gcagcgtaac gctgtagagg gccccgaaaa
      361 cgccctgccc gcccacggcg agtgaaaaca gctgagggtt cgagtaccgg ctggcgcgcc
      421 gcaactcgcc gtccggggtg acgagggtga gcgattcgac atgcgtcacc gccgtgcgtc
      481 cgtcgggacc ggctgcgttc ctcgcgatgc tttctcccac cgtcggcatg gtggtgcgtg
      541 cgctcgcggc ccgcgcatcg ctggggcgca agcgtttcgc aagcgtcgtc caggccgagc
      601 tcgcctggac ctcgacgagc cccaggtcct catctacccg aagaatccga tcgagccgca
      661 acgggtcata tgatttcgag ccgcgcaccg catcgcgcaa ctccaaggcg gacaacggga
      721 caaatgacat gccgctcatg agaccctccc cgccgacaat ctagccgaac ggcgggagcg
      781 cgactagagc gtcgcgcgca actcgaactg cgcgcaggcc ggcgcgtcgt cgaggcctgc
      841 aacctcgagc gcatcgaccc gcgcgcccgc ggggccgcga gccgcccacg agacgatcgc
      901 ctcgacggct ggcggcgaac cctgtacgag cgcctcgacg ctgccgtccg agcgattacg
      961 cacccagccg gtgatgccga gctttagggc ttcgtcctgc agggcggcgc gatagccgac
     1021 gccctggacg cgccctctga cacgaatttg ccgggtcacc atgcaggcga gcttggctga
     1081 tgttaagctg aaaagaggag gaacaaagag atgcggatcg gcttgctgct ggttgcggga
     1141 ctcgccctga tcgggttcct ggtagtggcc gtagtgttgc ctcagatggc gggcgcggaa
     1201 gcgagggatg ccgcccaggc gctgatcgcc ggagccgagc cggccaggca gcagatcagc
     1261 gccaccgcag aaaaatccgc tcgcctggcg ggcgccggca aaggcatcaa gatcgcgccc
     1321 aagaccgatc ccaaacacgg cgacatgaag tggatcgttg gcgacgacgg ctcggttcgc
     1381 ggctggaacg agaaaaatgc gctcgaaatc gcgctcaccc ccacgttgca aggcggcaag
     1441 gtgagctgga actgcaaggg ctacccggtc aacgcgatgc cggcaagctg cggcgggcgg
     1501 taacagcagt gagaattccc tggatgatcg cggtaggcgg cggcggacag cctggcaccg
     1561 cgacgtcgac gggtatcacg ttcgctaccg ccccgcggct cgcgtagctt tcgccgaata
     1621 tcccgccgtc acatccgcag tcgccgatcg cgaccacgag cttcggctcc ggggtcgcat
     1681 cgtaggtacg gcgcagtgcc gtctccatgt tgcgcgacac cggcccggtg acgagcagca
     1741 ggtcggcgtg gcgggggctg gccacgaagc ggatgccgag gcgctcgagg ttgtagtggg
     1801 ggctgttgca ggcgtgaatc tcgagctcgc agccgttgca cgaaccggcg tcgacatggc
     1861 ggatcgcgag cgatcttccg aagtgctcga gcacgaccct ttccaggcgc tgctcaagct
     1921 cgcgcatcga ttcgtcgggg ccgggcgctc gctcgctgac gatgccggtt ttcgcaatct
     1981 gcttgagaag ctggtacatg cctagaggtc gtggccgctg taagagagat tgaaagactt
     2041 attgatgagc gggaaatcgg gcacgatatt ccccagcacc gcgcgctcga gcaccggcca
     2101 gttctgccaa gaggggtcat gcgcatggac tcggtgtggc ctgcccccat tgcccatgtc
     2161 gagagcgatg aacacctcgc cgcgccagcc ctcgatccag ccgcagccac gccgtcccgg
     2221 ttgcattgca gggagagcgg cccgaagctc gccgccgcac aggcgctccg cgagcgcctc
     2281 gatcaggcgc agcgattcgg cgagctcctc gaagcgtacc gagatccgag cggcgacgtc
     2341 gccacgtatg tgctccccga ttctcacggc gagcgcgtcg tacggcggca ccgggtaatc
     2401 gcagcgcagg tcgtgcgcct ggccgctcgc ccgcccggcg aggccggtca gcccaaggcg
     2461 ctcggcgagc tccggcgtca cctgcccggt caggatgaaa cggtcctgcg cgccggcatg
     2521 ctcgtcgtag atcgcgcgca gcgttgcgac ttccgcgcca atctcaccta gcttttcaac
     2581 aaggatctcc ggtgcaagga gatcgcgggc gacgccaccg ggcacgatga agtccatcag
     2641 gtaacggtgc ccgaacagct cgtggtgcag ccgcagcagc tcttctttca gccgccagaa
     2701 ctgggaaaaa ccgaacgaga gggcgacgtc attgccgaga tagccgaggt cgccgagatg
     2761 gttcgcgacg cgctcgagct cgagcgcgat gccgcgcagc gcgacggcgc gcggcggcgg
     2821 ctcgacaccg ctcgccgcct cgagcgccat ggcataggcc caggcgtagg ccacggtgga
     2881 atcgccgctc acgcggccgg cgaggcgcgc gccgtcctcg gggccgagcg actcaaagcg
     2941 cttctcgatg cccttgtgct tgtagcccag acgctcctcg agccgcagca ctttctcgcc
     3001 cacgatggag aagcggaagt gccccggctc gatggtgccg gcgtgcaccg gaccgacggg
     3061 aatctcgtgc acgccttcgc cttcgacccg cacgaacgga tagcggtctt cgccaggcgc
     3121 gaagcccgcc gcggcgtcga agtccttgcg tagcgggaac tcgccgccgg gccacgcccc
     3181 atgccgcagc cacttgcgat ggtcgggatt ctcgtgtgcg tgcacgccga cgaggtcgta
     3241 ggccgcgcgc tgcatgcggt tggcggccgg gaagatgtcg gctatcccgg agtagcgggg
     3301 atgctcccgc tcaagcggca cggtgagcca caccaggccg gggcgcaagg cgagggtgac
     3361 gtgaagcgcg tagccggcgc cgcgcggcgt ctcgtcgctt ccccacagag cgaccaggcg
     3421 cgccttcagt gcgcgtgctt tctccgcaat gccgcgcaat gcaccgcgct cgatcgaggc
     3481 gtgccacgcc ggaagcgggc cgggcaggcg ctgcgcgtcg atgatggcca gatcagtaaa
     3541 ggggtgcgtc atccgatgaa agccgcggcc tggcggtacc agtcgacgag atagggcgga
     3601 atgtacaggc cgagcatcag cacgatcgcg aggtgcacga acaccgggac gagcgccggc
     3661 gggtgcggca gccgctgtac gcttgtctcg ccgaagatca tcggctgtac cttgccgaaa
     3721 atcgctgcga aggcgatacc gagagagatc agcaggaagg gcgtggccca cgggtgctcg
     3781 cgcatcgccg tcgtcaggac caagaattcg ctcgtaaaca cgccgaaggg cggcatgccg
     3841 agtatcgcga tagtccccag cgcaagcccc cagccgatgg tcggactgtg ctggatcagg
     3901 ccgcggatgg aggccatgtc ctgggtgccg caggcttgcg aggcgtggcc gacggtgaag
     3961 aagatcgccg acttggtgag cgagtgcacc gtcatgtgga gcaggcccgc gaacgaggcc
     4021 accggcccgc ccatgccgaa agcgaaggtc atgatgccca tgtgctcgat cgacgagtac
     4081 gcgaacatgc gcttgatgtc cttctgccgc gacaggaaga acgcggccac gaccaccgag
     4141 acgaggccga agcccatcat cagcccgccc gagaaattgc gctccagcgc gccgtcgacc
     4201 agcaccttgc cgcgcaccac cgcgtagagc gcgacgttga gcagcaaccc cgagagcacc
     4261 gccgaaatcg gcgtcgggcc ctcggcatgc gcgtcgggca gccagttatg cagcggcacg
     4321 agccccacct tggtgccgta gccgaccagc aggaacacga acgacaggga aagcaccgtc
     4381 ggctcgagct cgccgcgtac ctcgtgcaga tgcgtccaga gcagcgcgtc gccgcccggc
     4441 ccgagcacct tctcggcggc gaaatacagc aggatggtgc cgaacagcgc ctgcgcgatg
     4501 ccgaccgagc agaggatgaa atatttccac gccgcctcca ggctcgcggg cgtgcggtac
     4561 agggatacga gaagcaccgt cgacagggtc gcgccttcca tcgccaccca cagcatgccg
     4621 acgttgttcg acagcagcgt cagcagcatc atgaaggtga acagctgata catgctgtgg
     4681 tagagccgca ggcgcgagct gttgacgcgc ttgtggagct cctcggtgcg catgtaaggc
     4741 cgcgagaaca gcgccgtggt gaaggccacc agcgccgtta gcgccaccag gaacacgttg
     4801 aacgggtcga cgaacacctg ctcgttgagc gccgtcatcg ggccctccgc gatcacgcgc
     4861 gccgtgagca gcgtggctgc cgcgagcgtg ccgaggctca tgaaggcgct ggcctccgcc
     4921 gccca
//
LOCUS       DASPXW010000168         2550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1185311, whole genome shotgun sequence.
ACCESSION   DASPXW010000168 DASPXW010000000
VERSION     DASPXW010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185311"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..803)
                     /locus_tag="VEL04_03965"
     CDS             complement(18..803)
                     /locus_tag="VEL04_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HYT47553.1"
                     /translation="MNAPAPSLLELQHAVRRSVVARDDAEAGAHVVAGGIEPAARLAI
                     YRNTFASVLTNALRLSYPAVHRLVAAECFEGAARSFIEEQPPQCANLDDYGAGFPEFL
                     ARFAPVAALAYLPGVARLEWAVNRALHAQDAEPLDIARLAALTEDEQARVRFAPHPGV
                     GLVRADHPADAIWRAVLNQDDAALASIDLAGGPVWLLVHRAETGVDVSRLSEGAWRFT
                     AALLAGLPLHRALEEARCTHAQAVLAEHLAGGRFAGFSLAEAS"
     gene            complement(775..1668)
                     /locus_tag="VEL04_03970"
     CDS             complement(775..1668)
                     /locus_tag="VEL04_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HYT47554.1"
                     /translation="MRQDPGRQAEGHLNPIPAKAGIGLRFPHHQAVADTRPAVAWLEV
                     HTENYMGGGAPLRYLEAIRRDYPLSLHGVGLSLGSAEGLDAAHLERVRRTVERLEPGL
                     VSEHLSWSFVGGTYLADLLPLPMTEEALAVVCRHVEQVQACLKRRILVENPSTYLQFR
                     HSTLPEWEFLARVAERTGCGILCDVNNLYVSACNHGWDASAYLAALPPAAIGEIHLAG
                     HAVRKLDDGRTLRIDDHGSRVAPEVWALYAEALARFGAVPTLIEWDTDVPPLEVLIDE
                     AARAAAVLEETDRERPCAIAA"
     gene            complement(1629..1949)
                     /locus_tag="VEL04_03975"
     CDS             complement(1629..1949)
                     /locus_tag="VEL04_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012732768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HYT47555.1"
                     /translation="MNRYVLAASTLSSAVGLALAMQAAPTLAQGKMDMSNMPQVVKDN
                     MARMEKNKLQKCFGINAVAKNDCAEGAHSCAGQATKARDPQSFVLLPAGDCAKIQGGK
                     LKAT"
     gene            complement(2070..2282)
                     /locus_tag="VEL04_03980"
     CDS             complement(2070..2282)
                     /locus_tag="VEL04_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDCCVxC domain-containing (seleno)protein"
                     /protein_id="HYT47556.1"
                     /translation="MSEPILDSVLTCPKCGFSKTERMPTDACQFYYECTGCRAVLRPN
                     PGDCCVFCSFGSVKCPPVQAQRSCCG"
     gene            complement(2289..>2550)
                     /gene="merP"
                     /locus_tag="VEL04_03985"
     CDS             complement(2289..>2550)
                     /gene="merP"
                     /locus_tag="VEL04_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003116826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045340 - mercury ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mercury resistance system periplasmic binding
                     protein MerP"
                     /protein_id="HYT47557.1"
                     /translation="FLAAAAALAAPAFAADKTVALSVPGMYCELCPLTVKKALSKVPG
                     VSSVKVSYDKKEAVVRFDDAKTSVDALTNATANAGYPSSLKQ"
BASE COUNT          405 a          832 c          898 g          415 t
ORIGIN      
        1 tggcaatctc ctcgctgtca cgaagcttcg gccaggctga atccggcgaa gcgtccgccc
       61 gcgaggtgct ccgccagtac cgcctgggcg tgggtacagc gcgcctcctc cagcgctcga
      121 tgcagcggga gcccggcgag cagagcggcg gtgaaacgcc aagcgccttc gctcaagcgg
      181 ctcacgtcga cgccggtttc cgcgcggtgc acgaggagcc agactggacc gcccgcgaga
      241 tcgatcgacg cgagcgcggc atcgtcctga ttcagcaccg cgcgccagat ggcgtcggcg
      301 ggatggtcgg cgcgcaccag acccacgcca ggatggggcg caaagcgcac gcgcgcctgc
      361 tcgtcttcgg tgagcgcggc aaggcgcgcg atgtcgagcg gctccgcgtc ctgggcgtgg
      421 agcgcgcggt tcaccgccca ttcgagccgc gcgacgccgg gcagataggc gagggcggcg
      481 acgggcgcaa accgcgccag aaattccggg aagcccgcgc cgtagtcatc gaggttcgcg
      541 cactgcggcg gctgctcctc gatgaaagag cgcgcggcgc cctcgaagca ctcggcggcg
      601 acaagccggt gcaccgcggg atacgagagc cgcagcgcgt tggtgaggac gctcgcgaat
      661 gtgttgcggt agatggcgag gcgcgccgcc ggctcgatgc cgccggcgac gacgtgggcg
      721 ccggcttcgg catcgtcgcg cgcgacgacg ctgcggcgta cggcgtgctg gagctcaagc
      781 agcgatggcg caggggcgtt cacggtcggt ttcctcgagc accgcggcgg cgcgtgccgc
      841 ctcgtcgatt agcacttcga gcggcggcac gtcggtgtcc cattcgataa gggtcggcac
      901 cgcgccgaag cgcgcgagcg cttcagcgta gagtgcccag acctcgggcg cgacgcgcga
      961 gccgtgatcg tcgatgcgga gcgtgcgccc gtcgtcgagc ttcctcacgg cgtggccagc
     1021 gagatggatc tcgccgatcg cggcgggcgg cagcgcggcg agataggccg acgcgtccca
     1081 gccgtgattg caggcgctta cgtagaggtt gttgacgtcg cacagaatgc cgcagccggt
     1141 gcgttcggcg acccgggcga ggaactccca ctccggaagc gtcgaatgcc ggaattgcag
     1201 gtaggtcgac gggttctcga ccagaattcg ccgcttcagg caagcctgga cctgctcgac
     1261 gtgccggcat acgaccgcga gtgcttcttc ggtcatcggc aggggaagca gatcggcgag
     1321 ataggtgccg ccgacgaagc tccacgacag atgctcggag acgagtcccg gctcgagccg
     1381 ctcgaccgtg cgccgcacgc gctccaggtg cgctgcatcg agcccctccg cgctgccgag
     1441 cgacaggccg acgccgtgca gcgaaagcgg atagtccctg cggatcgcct cgaggtagcg
     1501 cagcggtgct ccgccaccca tgtaattctc ggtgtggacc tcgagccacg ccaccgcggg
     1561 acgcgtgtcg gcgaccgcct ggtgatgcgg gaagcgcaac ccgatcccgg ccttcgcggg
     1621 gatcgggttc aggtggcctt cagcttgccg ccctggatct tggcgcagtc gcccgcgggg
     1681 agcagcacga acgactgggg atcgcgcgct ttcgtggcct ggccggcgca ggagtgggcg
     1741 ccttccgcgc agtcgttctt cgcgacggcg ttgatgccga agcacttctg cagcttgttc
     1801 ttctccatcc gcgccatgtt gtccttcacg acttgcggca tgttgctcat gtccatcttt
     1861 ccttgcgcga gggtcggcgc ggcttgcatt gccagggcga ggccgaccgc actggaaagg
     1921 gtcgaggcgg cgagtacata tcggttcatg gtgggatatc tccggtcagt ggatatggtt
     1981 ggtgccggca gctcaagaaa aatcatccgg cacccccatt agaccgcgcg acggccgatt
     2041 tccttacaaa ggcgccgggt gctcgcgatt cagccgcagc aggagcgttg cgcctgaacc
     2101 ggcgggcatt tcaccgagcc gaacgagcag aagacgcagc agtcgcccgg attgggccgc
     2161 agcaccgccc ggcagcccgt gcattcgtag tagaactggc aggcgtcggt cggcatgcgc
     2221 tcggtcttgc tgaagccgca cttcgggcag gtcagcaccg aatcaaggat tggctcgctc
     2281 acgcttgact attgctttag gctcgacgga tagccggcgt ttgcggtcgc gttggtcagc
     2341 gcgtctaccg aggtttttgc gtcgtcgaac ctgacgaccg cttccttttt gtcgtagctc
     2401 accttcacgc tcgacacgcc gggcaccttg gacagcgcct tcttgactgt gagcggacag
     2461 agctcgcagt acatccccgg cacgctcagc gcgacggttt tgtcggccgc aaacgctggc
     2521 gcggcgagag cggcggccgc ggcgaggaac
//
LOCUS       DASPXW010000169         4832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_301060, whole genome shotgun sequence.
ACCESSION   DASPXW010000169 DASPXW010000000
VERSION     DASPXW010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4832
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301060"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VEL04_03990"
     CDS             complement(<1..423)
                     /locus_tag="VEL04_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47558.1"
                     /translation="MKSVGPAALAALLALAAGPAGAQEAPEAVYLKLHRAALVHNLDE
                     MLLYASDARRAELAGTAGSSVMLKLISSMMPRVYTVRSTAVTPDGLRAQMRATGSFSF
                     MGSTAPSYGTVDLVKQKGEWKVDRFEWSGDKPAGFDTVQ"
     gene            complement(420..1304)
                     /locus_tag="VEL04_03995"
     CDS             complement(420..1304)
                     /locus_tag="VEL04_03995"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYT47559.1"
                     /translation="MEADRKTTVVFADLIDATKLYGAGGDVAAHEALARCFEHLQGAV
                     APCGGRLVRESGDKVMVLIGTPDAAADAAVAMHTAVKQFPPVGKARPGLGVGFHYGAV
                     INEANGVFGDTVNLASRLVELAANGQIITTEETAKQLSSLYRACLRRLGPTSIRGRSD
                     EVAICELAWRTDENATNFLKNRAKARLVNATLTLSYRDRKIVRRREKDAITLGRGLDC
                     GLVIADDKASRQHCNIERRQDKFVVTDLSSNGTYVSIDGEEEVELNREELTLRKHGWI
                     AFGQPRASAKEAVAFSCE"
     gene            complement(1368..1493)
                     /locus_tag="VEL04_04000"
     CDS             complement(1368..1493)
                     /locus_tag="VEL04_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47560.1"
                     /translation="MELASTVGLVAVVLIVVVAAGYAVAEGSGEAAPAVAAEAQR"
     gene            1601..2761
                     /gene="pobA"
                     /locus_tag="VEL04_04005"
     CDS             1601..2761
                     /gene="pobA"
                     /locus_tag="VEL04_04005"
                     /EC_number="1.14.13.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018659 - 4-hydroxybenzoate
                     3-monooxygenase activity [Evidence IEA];
                     GO_process: GO:0018874 - benzoate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HYT47561.1"
                     /translation="MRVQVAIIGAGPAGLLLGQLLHRAGIDGVILESRSQEYVLARIR
                     AGVLESGTVALLERAGVASRLRAEGLIHAGFDLAFGGRRLRIDLEGLAGKSVTVYGQT
                     EVTKDLMEARAAAGAPAFYEARDVAVHAFDSEKPWVSFNSSQKIACDFIAGCDGFHGV
                     CRASVPAEKLKLFEKNYSFGWLGILSDTPPVSEELIYVSHERGFALCSQRSRTRSRYY
                     LQCPLSEQVDEWPDERFWAELRRRLPGDAAAQLVTGPSIEKSIAPLRSFVAEPLRFGR
                     LFLAGDAGHIVPPTGAKGLNLAASDVHYLAEALKNYYQTGSMEELETYSSRSLARVWK
                     AERFSWWMTKLLHKFPEEGGFGRRMQLAELEYIAGSRAAQAALAENYVGLPY"
     gene            2795..4591
                     /locus_tag="VEL04_04010"
     CDS             2795..4591
                     /locus_tag="VEL04_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HYT47562.1"
                     /translation="MKPVSRWLRAFGAALARKDIDAATSLFEADGYWRDLVAFTWNIK
                     TCEGQAEIKAMLAATLSHVNPSAWAIHGSPMTSEGLTEAWFSFETAVGRGIGHIRLRG
                     GSLRGAKCWTLLTALQELKGYEEKSGPTREAGVEHGALVDRRSWLERRQEEAAALGTT
                     RQPYCVIVGGGQGGIALAARLKRLQVPAIVVEKNARAGDSWRKRYRSLCLHDPVWYDH
                     MPYLPFPEHWPVFSPKDRIGDWLEMYVNVMELDYWSSTECTSARWDEEAGEWEVSLLR
                     NNLEITVRAKHLVLATGMSGFPDMPGIPGAGAFKGTLVHSSRHDGGAAWSGKSCVVLG
                     SNNSAHDIAADLWEQGASSVTMIQRSPTVVVRSELLMELAWGKLYSEAAVRAGITTEL
                     ADLTVASVPFRLLPALQKPIYDEIRRRDAALYAGLEKAGFMFHMGEDGSGLHSIYLRR
                     GAGYYIDVGACQMVIDGRIKLKSGVQVDALKENSVLLSDGTELPADLVVCATGYGSMN
                     QWAAQLISQQAADRVGKCWGLGSATKYDPGPWVGELRNMWKPTQVAALWFHGGNLMQS
                     RHYSLYLALQLKARMESIPTPVYYLEPVHHLK"
BASE COUNT          764 a         1647 c         1601 g          820 t
ORIGIN      
        1 ctgcacggtg tcgaagcccg ccggcttgtc gcccgaccac tcgaagcggt cgaccttcca
       61 ctcgcccttc tgcttgacga ggtcgaccgt gccgtagctc ggcgcggtcg agcccatgaa
      121 ggagaagctg ccggtggcgc gcatctgcgc cctcaggccg tcgggcgtga cggccgtcga
      181 tctcaccgtg tagacgcgcg gcatcatcga cgagatcagc ttcagcatca ccgagctgcc
      241 ggcggtgccg gcgagctcgg cgcggcgcgc gtcgctggcg tagagcagca tctcgtcgag
      301 gttgtggacc agggcggcgc ggtgcagctt caggtacacc gcctcgggtg cctcctgcgc
      361 gccggccggc cccgccgcga gggcgaggag cgcggcgagc gctgcgggcc cgacgctttt
      421 cactcgcagg aaaacgcgac cgcctctttc gcgctcgcgc gcggctggcc gaaggcgatc
      481 cagccgtgct tgcgcagcgt cagctcttcg cggttgagct cgacctcttc ttcgccatcg
      541 atgctgacgt aggtgccgtt cgagctcagg tcggtgacca cgaacttgtc ctggcgccgc
      601 tcgatgttgc agtgctggcg cgaggccttg tcgtcggcga tcaccaggcc gcagtccagg
      661 ccgcggccga gcgtgatggc gtccttctcg cgccggcgca cgatcttccg gtcgcggtag
      721 ctgagggtga gcgtcgcgtt gaccagcctg gccttggcgc ggttcttcag gaaattcgtc
      781 gcgttctcgt ccgtgcgcca ggccagctcg cagatcgcga cctcgtccga cctgccccgg
      841 atcgaggtcg gccccaggcg ccgcaagcac gcgcggtaca gcgagctcag ctgcttcgcg
      901 gtctcctcgg tggtgatgat ctggccgttg gccgccagct cgaccagcct ggaagcgagg
      961 ttgacggtgt cgccgaacac gccgttcgcc tcgttgatca ccgcgccgta gtgaaagccg
     1021 acgcccagcc cgggccgggc cttgccgacc ggcggaaact gcttgactgc ggtgtgcatg
     1081 gcgaccgcgg catccgccgc cgcatcgggc gtcccgatca gcaccatcac cttgtcgccg
     1141 ctttccctga cgaggcgtcc gccgcagggg gccacggcgc cctgcaagtg ctcgaagcag
     1201 cgcgcgagcg cctcgtgcgc tgccacgtct cctccggcgc cgtacaactt cgtggcgtct
     1261 atcaggtcgg cgaataccac cgtcgttttg cgatccgctt ccatcgctcc cgcgacgcta
     1321 gccgagaagc ggcccggcgg caaccaccgg ccgccttcct atcgctatta ccgctgcgct
     1381 tcggctgcga ccgccggcgc tgcttcgccg ctgccttcgg caacggcgta gcccgccgcc
     1441 accaccacga tcaggacgac ggccaccagc ccgacggtac tagcaagttc cacggtatgc
     1501 ccctttattc cagtcgtccc ctgagctgca ttctccccgc cgcggttcgc cgcgtcagcg
     1561 cacttcgtca cgcgcagccg gatgggcgtt agcatttcac atgcgcgtgc aggtagcgat
     1621 catcggcgct ggtccggccg gactacttct cggccagctc ctgcaccgcg ccgggatcga
     1681 cggcgtgatc ctcgagagcc gctcgcagga gtacgtgctc gcccgcatcc gcgccggggt
     1741 gctggagagc ggcacggtcg cgctgctcga gcgcgccggc gtcgcctcgc ggctgcgcgc
     1801 agagggatta attcacgccg gcttcgacct tgccttcggc ggccggcgcc tgcgcatcga
     1861 cctcgaaggc ctcgccggga aatcggtcac ggtctacggc cagaccgagg tcaccaagga
     1921 cttgatggag gcgcgcgccg ccgcgggggc gccggccttt tatgaggcgc gcgacgtcgc
     1981 ggttcacgcc ttcgattccg aaaagccctg ggtcagcttc aatagttcac agaaaatagc
     2041 atgcgacttc atcgccggct gcgacggctt ccacggcgtg tgccgcgcca gcgtgccggc
     2101 cgagaagctg aagctgttcg agaagaacta ctccttcggc tggctcggca tcctctccga
     2161 cacgccgccg gtgtcggagg agctgatcta cgtctcgcac gagcgcggct tcgcgctctg
     2221 cagccagcgc agccgcacgc gcagccgcta ctacctgcag tgcccgctct ccgagcaggt
     2281 cgacgagtgg cccgacgagc gcttctgggc ggagctgcgc cgccgcctgc ccggggacgc
     2341 cgcggcgcag ctcgtcaccg ggccctcgat cgagaagagc atcgcaccgc tcaggagctt
     2401 cgtcgccgag cccctgcgct tcgggcgcct gttcctcgcc ggcgacgccg gccacatcgt
     2461 gccgcccacc ggcgccaagg gcctgaacct ggcggcgagc gatgtgcatt atcttgccga
     2521 ggctttaaag aattattatc aaacgggaag catggaagaa ctcgaaacct attcctcccg
     2581 ctctctcgcc cgcgtctgga aggccgagcg cttctcgtgg tggatgacca agctgctgca
     2641 caagtttccc gaggagggcg gcttcggccg caggatgcag ctcgccgagc tcgaatacat
     2701 cgccggctcg cgcgccgcgc aggccgcgct cgccgagaac tacgtcggcc tgccttacta
     2761 aatggtaaat ggggacagac cccatttttc tcatttgaag ccggtctcgc gctggctgcg
     2821 ggccttcggg gctgcgctcg cgcgcaagga catcgacgcc gcgacgagcc ttttcgaggc
     2881 ggacggctac tggcgcgacc tcgtcgcctt cacctggaac atcaagacct gcgaggggca
     2941 ggccgagatc aaggcgatgc tcgccgccac gctctcgcac gtgaatcctt ccgcttgggc
     3001 gattcatggc agtcctatga ccagtgaagg cttgaccgaa gcctggttca gcttcgagac
     3061 cgcggtgggc cgcggcatcg ggcacattag gctgagaggc ggctcgctca ggggcgcgaa
     3121 atgctggacg ctcctcacgg cgctgcagga gctcaagggc tacgaagaga aaagcgggcc
     3181 gacgcgcgaa gcgggcgtcg agcacggcgc gctggtcgac cgccggagct ggctcgagcg
     3241 caggcaggag gaggccgcgg cgctcggcac cacgcgccag ccctactgcg tgatcgtcgg
     3301 cggcgggcag ggcggcatcg cgctcgccgc gcgcctgaag cgcctgcagg tgccggcgat
     3361 cgtggtcgag aagaacgcgc gcgccggcga ctcctggcgc aagcgctacc gctcgctctg
     3421 cctgcacgac ccggtgtggt acgaccacat gccgtacctc ccgtttcccg agcactggcc
     3481 ggtgttctcg cccaaggaca ggatcggcga ctggctggag atgtacgtga acgtgatgga
     3541 gctcgactac tggagctcga ccgagtgcac gtctgccagg tgggacgaag aggcaggcga
     3601 gtgggaagtt tctcttttac gaaataacct ggaaatcact gtgcgagcaa aacacttggt
     3661 cctcgccacc ggcatgtcag gctttccgga catgcccggg attcccggcg ccggggcatt
     3721 caagggcacg ctcgtccact ccagcaggca cgacggcggc gcggcctgga gcggcaagag
     3781 ctgcgtcgtg ctcggctcga acaactccgc gcacgacatt gccgcggacc tgtgggagca
     3841 aggcgcatcg tcggtgacga tgatccagcg ctcgcccacg gtggtggtgc gctcggagct
     3901 gctgatggag ctcgcctggg gcaagctcta ctcggaggcc gcggtgaggg cgggcatcac
     3961 caccgagctc gccgacctca cggtggcctc ggtgcctttc aggctgctgc ccgcgctgca
     4021 gaagccgatc tacgacgaga tccgcaggcg cgacgccgcg ctctacgcgg gcctggagaa
     4081 ggcgggcttc atgttccaca tgggggagga cggctccggc ctgcactcga tctacctgcg
     4141 ccgcggcgcc ggctactaca tcgacgtcgg cgcgtgccag atggtcatcg acggccgcat
     4201 caaactaaaa agcggagtgc aagtcgacgc gctcaaggaa aattctgtcc tcctgtcgga
     4261 cggcacggag ctgcctgccg acctggtggt ctgcgccacc ggctacggct cgatgaacca
     4321 gtgggcggcg cagctcattt cgcagcaggc cgcggaccgg gtcggaaaat gctggggcct
     4381 gggctccgca accaagtatg atcccggccc ctgggtgggc gagctgcgca acatgtggaa
     4441 gccgacgcag gttgccgcgc tctggttcca cggcggcaac ctgatgcagt cgcgccacta
     4501 ctcgctctac ctcgcgctgc agctcaaggc gcgcatggaa agcatcccga ctccggtgta
     4561 ctacctcgag ccggtgcacc acctcaagtg aacggaggca gcatgccacg cagcgcggtg
     4621 aaaaatgggg tcagtcccca tttttgacct gggtcacccc ttgagggctt tcttcaccag
     4681 gtccaggcat tcatcgaagc tcagccggta gtcgagcacg tccagctcgg cgaaagtctt
     4741 ccgcaggatt ttgtcgcttg aggcgagacg tccgaggatg atatcgcgct gcttgcggag
     4801 cagcggcttg attcgggcaa tttccttggg ct
//
LOCUS       DASPXW010000170         4566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_51392, whole genome shotgun sequence.
ACCESSION   DASPXW010000170 DASPXW010000000
VERSION     DASPXW010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51392"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1760)
                     /locus_tag="VEL04_04015"
     CDS             complement(<1..1760)
                     /locus_tag="VEL04_04015"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYT47563.1"
                     /translation="MLRLLALTASRRPRGRMALSVAGIALGVALGYGVHLVNRAAVSD
                     VAAAVRALAGEADLEVRGARAGFSESLYPRIARVAGVAVASPALELDVGVAGAERTIR
                     VIGLDILRAALLQPQLALGDRLELLSPDKVLLSAAAANALRLARGDKLSLVVGQRRVA
                     LDVAGVLPASSLHGVAALTDVATAQWRLGRLGELNRIDIRLSSSVNRNEIAKTIQALL
                     PPGVHVSAVESLEQASEYPSRSYRVNLNVLALVALFTGGFLVFSAQALEVARRRGEHA
                     LLRVLGLTQGGISRLVLLEAAALGAAGAAAGLVLGYALAAAAVRGAGADLGAGMFRGV
                     FPELEFSLLPAFFYLVAGIVVAIVGAVLPALDAARTAPARALKAGDEQVMFRRLSPAW
                     PGLALIAAGAALSQLGPVGGLPLFGYAAIACLLVGSIALMPRLSELIFKVFPKWNSVS
                     LTLALAQLRGAPGEAAVSLAATVASFSLMAAMAIMVASFRQSVDDWLDRVLPADLYFR
                     TTQAGDTAYLEPGFEIRVAALPGVARVDFLRSGRILLDAARPPLALIARDRAEKAFPL
                     VGKRHERQPADPMQVWISEAV"
     gene            1783..2643
                     /locus_tag="VEL04_04020"
     CDS             1783..2643
                     /locus_tag="VEL04_04020"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HYT47564.1"
                     /translation="MLRGVFIVILALFTGGVFAQEPPSWFAQSLLDLREDIAEAAREG
                     KRVMLYFEQAGCPYCKRLVEVNFRQPAIAAKMRRHFVSLDINIWGDRELTSPEGKAMT
                     EKQFAAALKIQYTPTLVFFDEKGRVALRINGYYPPERFAAALDRAVGRASQPSSSPAS
                     RRKTGGKPLAVIIEAPDCAACDELNQVTLKHDAVRAQLARFDVERLPLSGDRARALEV
                     GYVPTLVLFDAGGREVLRMEAYFRPFHVAGSLEYVSSGAYRREPSLQRFLQARADRIR
                     RQGTTVDLWN"
     gene            2671..3240
                     /locus_tag="VEL04_04025"
     CDS             2671..3240
                     /locus_tag="VEL04_04025"
                     /inference="COORDINATES: protein motif:HMM:NF028308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RT0821/Lpp0805 family surface protein"
                     /protein_id="HYT47565.1"
                     /translation="MRRALLVIPALVSLSLLPIPSPADPPPWAPAHGWRKKNDPNYVG
                     YTGKKWEKDYGVIEGRCNSQQIGAVLGGAVGGVIGSRVGEKENRPVAIVVGTVLGAVI
                     GANIGKQLDESDRACMGHALELAGENKTVVWTNQATGVNYQLTPTRNLGDRRQPCREF
                     TTLVKDGGKQHTVQGVACRRGQGEWVFKS"
     gene            3352..>4566
                     /locus_tag="VEL04_04030"
     CDS             3352..>4566
                     /locus_tag="VEL04_04030"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HYT47566.1"
                     /translation="MDNKVEHSSNPKTFGRYQIIDKLGRGAMGLVYRAIDPLIERELA
                     IKTLLPDLPEDIVAEVRERFLREARAAGKLSHPNIVTVFDVGEQDGVGYIAMELVAGR
                     SLQQILREPESLPFDTVAEIAAQVADALDHAHGHSIVHRDVKPGNVMVAPSGRAKLTD
                     FGIAWVPSSSMTQTGAALGSPKYMSPEQATGQPVEPRSDVFTLGVLLYEMLTRRNPFS
                     REDDVSPFDVMDRIAQEPHPPVREINPEVPAAFEQIIDRALAKKPEDRYASAAEMANA
                     LRSIGEKKDKPQTPKADAAAIDALMRVAAQYFGEFAKRLSSVRPTSDAPYEFLHIGSL
                     PVVTLGQAELEDQEKHLILRYRVTPRTPRKVTLAGEDIAHCKEYLTNLKVEFEARDGE
                     FTVTGSLPSEINL"
BASE COUNT          838 a         1563 c         1528 g          637 t
ORIGIN      
        1 acggcctcgc tgatccacac ctgcatcgga tcggcgggct ggcgctcgtg gcgcttgccg
       61 accagtggaa aggccttctc ggcgcggtcg cgggcgatga gcgcgagcgg cggccgcgcc
      121 gcatcgagca ggatgcggcc gcttctcagg aaatcgaccc gcgccacgcc cggcagcgcg
      181 gctacgcgaa tctcgaagcc cggctcgaga tatgcggtgt ccccggcctg cgtggtgcgg
      241 aaatagagat cggccggcag cacgcgatcg agccagtcgt ccaccgactg ccggaacgag
      301 gccaccatga tcgccatcgc ggccatcagc gagaagctcg cgacggtcgc cgccaggctg
      361 accgccgcct cgcccggcgc gccgcgcagt tgcgcgaggg ccagggtaag cgaaacggaa
      421 ttccatttag ggaatacttt gaagatcaat tctgaaaggc gaggcatgag cgcgatgctg
      481 ccgaccagga gacaggcgat cgccgcgtag ccgaagagcg gcaggccgcc gaccggtccg
      541 agctgggaaa gcgccgcgcc cgcggcaatc aatgcgagcc ctggccaggc tggcgagagg
      601 cggcggaaca tgacctgctc gtcgcccgcc ttgagcgcgc gcgcgggcgc tgtgcgcgcg
      661 gcgtccagcg ctggaagcac agcgcctact atggccacca caattcccgc aaccaggtaa
      721 aagaatgcag gcagcagtga aaactccagt tctggaaaga cacccctgaa cattccggcg
      781 cccagatcgg cgcccgcgcc gcgcaccgcg gcggcggcga gcgcgtagcc gagcaccagg
      841 ccggcagccg cgcccgccgc accgagcgcc gccgcctcga gcagcaccag ccgcgagatc
      901 cccccctgcg tcaggccgag cacgcgcagc agcgcatgct cgccgcgcct gcgcgccact
      961 tcgagcgcct gcgcggaaaa caccaggaag ccgccggtga agagcgcgac caaggcgagc
     1021 acattgagat tgacccggta ggagcgcgac gggtactcgc tcgcctgctc gaggctttcc
     1081 actgcagaca catgcacgcc cggcggcagc agcgcttgaa ttgttttcgc tatttcattt
     1141 cgattcaccg aggaagaaag gcgtatgtca atgcgattca attcaccgag acgcccaagg
     1201 cgccactgcg cagtcgccac gtcggtgagc gccgcgacac catgcagcga ggacgcaggc
     1261 agcacgcccg cgacgtcgag cgcgacccgc cgctgcccga cgacaaggct cagcttgtcg
     1321 cccctggcga ggcgtagcgc gttcgcggcc gcggccgaga gcagcacctt gtcgggcgaa
     1381 agcagctcca gccggtcccc gagcgcgagc tgcggctgca gcagcgccgc gcgcaggatg
     1441 tcgagcccga tgacccggat ggtgcgctcg gcgcccgcca cgccgacgtc gagctcgagc
     1501 gccgggctcg ccaccgccac gccggcgacg cgggcgatgc gcggatagag cgactcggag
     1561 aagccggcgc gcgccccgcg cacctcgagg tcggcctcgc cggcaagcgc gcgcaccgcc
     1621 gcggcgacgt cgctcacagc cgcgcggttc acgaggtgaa cgccgtagcc gagcgcgacg
     1681 ccaagcgcga tgcccgcgac cgagagcgcc atgcggccgc gcgggcgtcg tgaggcggtg
     1741 agggcaagga gccgcagcat cggcagctac tatactgtgg gcatgctgcg cggcgttttt
     1801 atcgtcatcc tggctctctt caccggggga gtttttgcac aagaaccacc atcctggttc
     1861 gcgcaatcgc tgctcgacct gcgcgaggac atcgccgagg cggcgcgcga aggcaagcgc
     1921 gtgatgctct acttcgagca ggccggctgc ccgtactgca agcggctggt cgaggtgaat
     1981 ttcaggcagc cggcgatcgc cgcaaagatg cggcgccatt tcgtctcgct cgacatcaac
     2041 atctggggcg accgcgagct cacctcgccc gagggcaagg cgatgaccga gaagcagttc
     2101 gccgccgcgc tgaagatcca gtacacgccg acgctggtct tcttcgacga gaaaggccgc
     2161 gtcgcgctgc gcatcaacgg ctactacccg ccggagcgct tcgctgccgc tcttgaccgg
     2221 gccgtcggca gggcaagcca gccttcttcc agtccagcct caagaagaaa aaccggtggc
     2281 aaaccgctcg cggtgatcat cgaggccccg gactgcgcgg cctgcgacga gctcaaccag
     2341 gtgacgctca agcacgacgc ggtccgcgcc cagctcgcca gattcgacgt cgagcgcctg
     2401 ccgctctccg gggaccgggc gcgcgccctc gaggtcggct atgtgccgac gctggtgctg
     2461 ttcgatgccg gcggacggga ggtgcttcgc atggaggcct atttcaggcc cttccacgtt
     2521 gccgggtcgc tcgaatacgt gtcgagcggc gcctatcgcc gcgagccttc gctgcagcgc
     2581 ttcctgcagg ccagggcgga tcgcatacgt cgccagggaa cgacagtgga cctctggaac
     2641 tgacctcggc ggttaatcta aaatcgggcc gtgagaagag ccctgctcgt catccccgcg
     2701 ctcgtttcgc ttagcctgct gccgatcccg tcgcccgccg atccgccgcc ttgggcgccg
     2761 gcgcacggct ggcgcaagaa gaacgacccc aattacgtcg gctacaccgg caagaaatgg
     2821 gaaaaggact acggcgtgat cgagggccgc tgcaacagcc agcagatcgg cgcggtgctc
     2881 ggcggcgcgg tgggcggcgt aataggctcg cgcgtcggcg agaaggagaa ccggccggtg
     2941 gcgatcgtgg tcggcaccgt gctcggcgcg gtgatcggcg ccaacatcgg caagcagctc
     3001 gacgaaagcg accgcgcctg catgggccac gcactcgagc tcgcgggcga gaataagacg
     3061 gtggtctgga cgaaccaggc gaccggcgtc aactaccagc tcaccccgac gcgcaacctc
     3121 ggcgatcgca ggcagccgtg ccgcgaattc accactctcg tcaaggacgg cgggaaacag
     3181 cacacggtgc agggcgtcgc ctgccggcgc ggccagggcg aatgggtgtt caagtcctag
     3241 tcatgcatct aagttcctaa gcacgccccc gaccgcagtt atccggccgt cctgaaccgc
     3301 gacatcaact tgcgggggcg tggcgccggt tccgtcgtac actttggcgc cgtggataac
     3361 aaggtcgagc acagcagcaa tcccaagact ttcggccgtt atcaaatcat cgataagctc
     3421 ggccggggcg cgatgggcct tgtttatcgc gcgatcgacc ccctgatcga gcgcgagctc
     3481 gcgatcaaga cgctgctccc cgaccttccg gaggatatcg tcgccgaggt gcgcgagcgt
     3541 ttcctgcgcg aagcgcgcgc ggccggcaag ctcagccatc cgaacatcgt caccgtgttc
     3601 gacgtcggcg agcaggacgg cgtcgggtac atcgcgatgg agctggtggc gggacgctcg
     3661 ctgcagcaga tcctgcgcga gcccgagtcg ctgccgttcg acaccgtcgc ggagatcgcc
     3721 gcgcaggtcg ccgacgcgct cgatcatgcc cacggccact ccatcgtgca ccgcgacgtg
     3781 aagccgggga acgtgatggt ggcgccaagc ggccgcgcca agctcaccga cttcggcatc
     3841 gcctgggtgc cgtcctcgag catgacgcag accggcgcgg cgctcggctc gcccaagtac
     3901 atgtcgcccg agcaggcgac cgggcagccg gtcgagccgc gctccgacgt gttcacgctt
     3961 ggcgtgctgc tctacgagat gctgacgcgg cgcaatcctt tctcgcgcga ggacgacgtc
     4021 tcgcccttcg acgtcatgga ccggatcgcg caggaacccc acccgccggt gcgcgagatc
     4081 aatcccgaag tgcccgccgc gttcgagcaa atcatcgacc gtgcgctcgc gaagaagccc
     4141 gaagatcgct acgcgagcgc cgcagagatg gcaaacgcgc tgcgcagcat cggcgaaaag
     4201 aaggacaagc cgcaaacccc gaaagcggac gcggcggcga tcgacgcgct gatgcgcgtg
     4261 gcggcgcagt atttcggcga attcgcgaag cgcctgagct cggtcaggcc gacctcggat
     4321 gcgccctacg agttcctgca catcggcagc ctgcctgtgg taactctggg ccaggcggag
     4381 ctggaggatc aggagaaaca cctcatcctg cgctaccgcg tgacgccgcg caccccgagg
     4441 aaggtgacgc tggcgggcga agacatcgcg cactgcaagg aatacctgac gaatctgaaa
     4501 gtggaattcg aagcgcgtga cggcgaattc acggtcaccg gcagcctgcc ctcggagatc
     4561 aacctg
//
LOCUS       DASPXW010000171         4292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_51954, whole genome shotgun sequence.
ACCESSION   DASPXW010000171 DASPXW010000000
VERSION     DASPXW010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51954"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="VEL04_04035"
     CDS             complement(<1..1038)
                     /locus_tag="VEL04_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HYT47567.1"
                     /translation="MEKLDAVVIGGGVVGLAVARALALAGRELVILESEDAIGTHASS
                     RNSEVIHAGIYYPKDSLKARSCVAGKELVYEYCVAHGVPHRRCGKLIVATDENQLNEL
                     QNIQKRAHANGVTDVVWMSRAQALALEPELQCVAALYSPSTGIIDSHALMLAYLGDAE
                     DRGAMLALKSTFQKAIVARDGLEVHVAGSDPIKTKILVNSAGLRAPTVARAMQGYHAE
                     LAPRELYAKGNYYSLNKKAPFSRLIYPVPEPGGLGVHVTLDLAGQARFGPDVEWIERI
                     AYDVDPRRAERFYAAIRRYWPALPDGALAPGYAGIRPKTAGPGEPASDFKIQGPRAHG
                     VAGLVHLFGIES"
     gene            complement(1025..2212)
                     /locus_tag="VEL04_04040"
     CDS             complement(1025..2212)
                     /locus_tag="VEL04_04040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HYT47568.1"
                     /translation="MSSAPAQTSSRMVVLLGALIALPALGTDLYVPSLPALAQSLGAP
                     VSAAQFTLTTYFIGLAAGQLLWGPLSDRYGRKPVLVAGLAMMLVSSIAASLMESVAGV
                     AAARLAQGFGMSSGALIGRTIVRDLHAYEQAARLLASMTIVFSLVPICAPLAGALLAS
                     AAGWHSVFVCMAAIAALLLISVTGLRETAPAERRSVHPLLIARTFAAILGDRRFVAPL
                     LLLLCAYAGILAWVSNSAFTLVRGLGAGTPAYGLMFAMVMLGQIGGAWLSSRLVLRLG
                     IARLQRFGVLVMLGAGAAAAALAWLDAGHWLAVVVPFMVFLFGTALVVSNATAAALTP
                     FPGSAGAASSLIGAIGFTMGALISTLLGAVFDGSARPMASVAAAAGLAAFLFERFLFR
                     GKA"
     gene            complement(2205..3563)
                     /locus_tag="VEL04_04045"
     CDS             complement(2205..3563)
                     /locus_tag="VEL04_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47569.1"
                     /translation="MKKIVRHLLFSAMFPAVFLIAADAAADWKLPPDGGTLSREEVNV
                     ILAADDFPESTAYDRKIEYLDFTSYGQKFTQVVVTLHPKQPRMHRGKKLVVVGGEPGS
                     EYAMDFLETPEGSEGMGVWLAKRGVTFVALTRIGRWNFLDRAGNGSWREIPLESRMPI
                     YHRGQQTYWSPDDYSIQKSATTTTATGGDSAVYRMPKPGTELYNQTLAAASPVTVLEG
                     YRLAIERAVPPRERRRTCVLYWGMSTGGAFLYPLAKQLKPDGYLGWGTSSTGLAYVYR
                     KAKQGDFGTPYARSALRVRERGLDDFEYYTRDVPAEVRQKWWQGALKEPRFKSIEDAP
                     MQFNIAALTEVALRLWLSDFLPEVERRAGLGSLIREILEPSFPPRELKGVAILDVNGT
                     RDEAIPPQIVDAHREIMEPYAGRYRVARIQDFHHYLFTQESIKAVGTLWLRFIESGYF
                     DF"
     gene            3562..>4292
                     /locus_tag="VEL04_04050"
     CDS             3562..>4292
                     /locus_tag="VEL04_04050"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HYT47570.1"
                     /translation="MPPILMPDLMRGFPPQPQNQVTLANWRSAPFNRWAFHHVREIVP
                     SADIPNAAGKVLALPSAPQRLPLGAFLEETDTDGLAVLHRGKLVFEHYANGMTAETPH
                     ILMSVSKSMLGLLVGGLGLELERRVSEVLPEIAQTAYRGATLRQLLDMRAGIAFDEDY
                     LATTGAIVEYRKATSWAPLGPGDTPSVLRSFYQNLKETDGAHGGRFHYVSPNTDLLGW
                     VIERATGRRFADLMSELVWKPIGAER"
BASE COUNT          738 a         1441 c         1441 g          672 t
ORIGIN      
        1 cgactcgatg ccgaagagat gcaccagccc tgcgacgccg tgcgcgcgcg gcccctggat
       61 cttgaagtcg gacgcgggct cacccggacc tgcggtcttc ggccggattc cggcatagcc
      121 cggcgcgagc gcaccgtcgg gcagggcggg ccagtagcgg cggatcgcgg cgtagaagcg
      181 ctcggcgcgc cgcgggtcga cgtcgtacgc gatgcgctcg atccactcga cgtccggacc
      241 gaagcgcgcc tggccggcga ggtcgagcgt gacgtgcaca ccgagcccgc ccggctcggg
      301 cactgggtaa atgaggcgcg aaaaaggagc tttcttgttc aaagaataat aattaccttt
      361 ggcgtagagc tcgcgcggcg cgagctccgc gtggtagccc tgcatcgccc tggcgacggt
      421 tggcgctctg aggcccgcgc tgtttacgag gatttttgtc tttatcggat cgcttccggc
      481 gacgtgcacc tcaagcccat cccgagcgac gattgccttt tgaaaggtac ttttcagtgc
      541 gagcatggcg ccgcggtctt cggcatcgcc gaggtaggcg agcatcagcg catggctgtc
      601 gatgatgccg gtcgaaggcg aatacagcgc cgcgacgcac tgcagctcgg gctcgagcgc
      661 cagcgcctgc gcgcggctca tccagacgac atcggtgacg ccgttggcgt gagctcgttt
      721 ttgaatgttt tgtaattcat tcagttgatt ttcatcggtt gcaacgatca gcttgccgca
      781 gcgccggtgc ggcacgccgt gcgcgacgca gtactcgtag acgagctcct ttccggcgac
      841 gcagctgcgc gccttcagcg agtctttcgg gtagtagatg ccggcgtgga tcacctcgga
      901 gttgcgcgag ctcgcatgcg tgccgatcgc gtcctcggac tcgaggatca ctagctcgcg
      961 gcccgcgagc gccagcgcgc gcgccacggc gaggcccacc accccgccgc cgatcaccac
     1021 tgcgtcaagc ttttccacgg aacaggaatc tttcgaacag gaatgccgcc agtcctgcgg
     1081 ccgccgcgac gctggccatg gggcgcgcgc tgccgtcgaa tacggcgccg agcagcgtgc
     1141 tgatcagcgc gcccatggtg aagccgatgg cgccgatcag cgaagacgcg gcgcccgcag
     1201 acccgggaaa cggcgtcagc gccgccgcgg tagcgttcga cacgacgagt gcggtgccga
     1261 agaggaacac catgaagggg accaccaccg cgagccagtg tccggcgtcg agccaggcga
     1321 gcgccgccgc cgcggcgccg gcgcccagca tgacaagcac gccgaagcgc tgcagccggg
     1381 cgatgccgag gcgcagcacc agccggctcg atagccatgc gccgccgatc tgcccgagca
     1441 tcaccatggc gaacatcagt ccgtaggcgg gcgtgccggc cccgagcccg cgcaccaggg
     1501 tgaacgcaga gttcgatacc cacgccagga tgcctgcata cgcgcacaga agcagaagca
     1561 ggggagcgac gaagcgccgg tcgccgagaa tggcggcgaa ggtgcgcgcg atcagcaagg
     1621 gatgcaccga gcgccgctcc gccggggcgg tctcgcgcaa gccggtgacc gaaatcagca
     1681 gcagcgcggc gattgcagcc atgcacacga acaccgaatg ccagccggcg gcgctcgcga
     1741 gcagcgcgcc cgcgagcggc gcgcagatcg gcaccaggct gaacacgatg gtcatgctgg
     1801 cgagcaggcg cgccgcctgc tcgtaggcgt gcagatcgcg cacgatggtg cgcccgatga
     1861 gcgcgccgct cgacatgccg aagccctgcg cgaggcgggc ggccgcaacg ccggccacgg
     1921 actccatcaa gctcgcggcg atcgaggaaa ccagcatcat cgcgaggccg gcgaccagca
     1981 ccggcttgcg gccgtagcga tccgaaagcg gcccccacag cagctgcccg gcggcgaggc
     2041 cgatgaagta cgtggtgagg gtgaactgcg cggcgcttac cggcgcgccg agcgattgcg
     2101 cgagcgcggg cagcgacgga acgtacaggt cggtaccgag cgcgggcagc gcgatcagcg
     2161 cgccgagcag caccaccatg cggctcgacg tctgcgcggg ggcgctagaa atcgaagtat
     2221 cccgactcga tgaacctcag ccacagcgtg cccacggcct tgatgctttc ctgggtgaac
     2281 aggtagtggt ggaagtcctg gatccgggcc acgcggtacc tgccggcgta cggctccatg
     2341 atctcgcgat gcgcgtcaac gatctggggc gggatcgcct cgtcgcgcgt gccgttgacg
     2401 tccaggatcg cgacgccttt cagctcacgc ggcgggaagc tcggctcgag gatttcgcgg
     2461 atcaggctgc cgagaccggc tcgcctctcg acctcgggca ggaaatccga cagccacagg
     2521 cgcagcgcca cctcggtcaa ggccgcgatg ttgaactgca tcggcgcgtc ctcgatgctc
     2581 ttgaagcgcg gttccttcag cgccccctgc caccacttct gcctgacttc ggcgggcacg
     2641 tcccgcgtgt agtactcaaa gtcgtcgagc ccgcgctcgc gcacccgcag cgccgatcgc
     2701 gcgtagggtg tgccgaaatc gccctgcttc gccttgcgat acacataggc gaggccggtg
     2761 ctgctggtgc cccagccgag atagccgtcg ggcttgagct gcttcgccag cggatagaga
     2821 aacgctccgc ccgtagacat gccccagtag agcacgcatg tccgccggcg ctcgcgcggc
     2881 ggcacggcgc gctcgatcgc gagccggtag ccttcgagca ccgtcaccgg cgacgccgct
     2941 gcgagcgtct ggttgtagag ctccgtaccg ggcttcggca tgcggtacac ggcgctgtcg
     3001 cccccggtcg ccgtcgtcgt ggtggcggac ttctggatcg aataatcgtc gggcgaccag
     3061 tacgtctgct ggccgcggtg gtagatcggc atgcggctct cgagcgggat ttcccgccac
     3121 gagccgttgc cggcgcggtc gaggaaattc cagcgcccga tgcgtgtcag agcgacgaag
     3181 gtgacgccgc gcttcgccag ccagacgccc attccctcgc tgccttcggg agtttcgaga
     3241 aagtccatgg catactcgct gcccggctcg ccgccgacca caaccagctt cttgccgcgg
     3301 tgcatgcgcg gctgcttcgg atgcagcgtc actaccacct gggtgaactt ctggccgtaa
     3361 gacgtaaagt cgaggtattc gatcttccgg tcataagccg tgctctcggg aaaatcgtcg
     3421 gccgcgagaa tgacattcac ctcctccctc gagagcgtgc ctccgtcggg cggcagcttc
     3481 cagtccgctg cagcatcggc ggcgatcagg aaaacggcag ggaacatagc gctgaagagc
     3541 aggtgccgga cgattttttt catgccaccg attctaatgc ctgacctgat gcgcggattt
     3601 ccgccgcagc cgcagaacca ggtgacgctg gcgaactggc gcagcgcgcc gttcaaccgc
     3661 tgggcgttcc atcacgtgcg cgagatcgtg ccttccgccg acattcccaa cgctgccggc
     3721 aaggtgctcg cgctgccatc tgcgccgcag cggctgcctc ttggagcatt tctggaggag
     3781 acggataccg acggcctcgc cgtcctgcat cgcggcaagc tggtcttcga gcattacgcc
     3841 aacggcatga ccgcggagac gccgcacatc ctgatgtcgg tctccaaatc gatgctcggc
     3901 ctgctggtcg gcgggctggg gctcgagctc gaacggcgag tgagcgaggt gctccctgaa
     3961 atcgcgcaga ccgcctatcg gggcgccacc ctccgccagc tactcgacat gcgcgcgggt
     4021 atcgcgttcg acgaggacta cctcgcaacc acgggcgcga tcgtcgagta ccgcaaggcg
     4081 accagctggg cgccactcgg gcccggcgat acgccgtccg ttttgcgctc cttctatcag
     4141 aacctcaaag aaacggacgg ggcgcacggc ggccggttcc actatgtctc gccgaacacc
     4201 gacctgctcg gctgggtgat cgagcgcgcc accgggcggc gcttcgcgga cctgatgagc
     4261 gaactggtgt ggaagccgat cggcgcggag cg
//
LOCUS       DASPXW010000172         3454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1186855, whole genome shotgun sequence.
ACCESSION   DASPXW010000172 DASPXW010000000
VERSION     DASPXW010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186855"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VEL04_04055"
     CDS             <1..333
                     /locus_tag="VEL04_04055"
                     /inference="COORDINATES: protein motif:HMM:NF021613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HYT47571.1"
                     /translation="ERHLDLMDVLSIYGGRGAPLDEISRLAGFPGKVGIGGDAVWETY
                     RRGAIAAIRDYCEADCANTYLLFLRFQLMRCALTKEQYARECSLLKSFIEKQNRPHWA
                     EFLSLWKN"
     gene            300..1619
                     /gene="rlmD"
                     /locus_tag="VEL04_04060"
     CDS             300..1619
                     /gene="rlmD"
                     /locus_tag="VEL04_04060"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HYT47572.1"
                     /translation="MGRIPVALEELILGIESLDAEGRGVARKPDGKAVFVEGALPGER
                     VQAGLVASKRSFDVFKSISVLQPSSGRRIARCPHFGVCGGCATQHADAPTQMAAKQRW
                     LEDSLQRIGKVAPETVLPIIYGEEWGYRHRARLSVRRVAKKGGVMVGFRERRSTHVAD
                     MRECPVLPAQVSALIPALKQLVEKLSVRERLPQIELAAGDNATALVFRHLLPLEAADQ
                     VFLQEFSERHGVHVWLQPGGPETAHAFHPVASELYYELPEFGLRIHFRPTEFTQVNPA
                     LNRILVSRALRLLEPQPGERVADLFCGLGNFSLPIASRGADVVGFEGSAELVERARAN
                     ASANGLVAQFQVTDLFKHGIAAFGPFDKLLFDPPREGAVELIKSLPEGWPRRIVYVSC
                     DPATLARDAGVLAHTKGFRLAAAGVANMFPHTAHVESIALFERGESP"
     gene            1616..2077
                     /locus_tag="VEL04_04065"
     CDS             1616..2077
                     /locus_tag="VEL04_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HYT47573.1"
                     /translation="MNAKHAVFAMAAALLAASAFAAPKIDSIVVKPNPAAFAGGKAPE
                     VEISVTVSRTRFDSGACDARVEFGDGEGRSVDFGVATTRTVRHVYKKGGNYRVVARGA
                     GKNPCEGTRDLAFKLTGGPAEKKAVKKTAAKKDAKKKGEKKKDEKNKDEPK"
     gene            complement(2117..2845)
                     /locus_tag="VEL04_04070"
     CDS             complement(2117..2845)
                     /locus_tag="VEL04_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HYT47574.1"
                     /translation="MEPELKPVRTGDQPFGRGTTYDAQEVALQAQGQKVLRNTYLLLA
                     LTMVPTVIGAFIGMATAGVIMASPIISSLVMLAVVIGLQFGIAANRNSAIGIGLLLLM
                     TGLLGWWLGPILNYALAMRNGMQLVGYAAVGTGVIFFAMGVVATTTKRDFSFMGKFLF
                     VGMIALLIAMFANMFLQIPALALTISTLVIVVFSLFLLYDLSRIIRGGETNYVIATTG
                     VYMSLFNIFANLLNILLALSGDRD"
     gene            2953..3040
                     /locus_tag="VEL04_04075"
     tRNA            2953..3040
                     /locus_tag="VEL04_04075"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2987..2989,aa:Ser,seq:tga)
     gene            3118..3360
                     /locus_tag="VEL04_04080"
     CDS             3118..3360
                     /locus_tag="VEL04_04080"
                     /inference="COORDINATES: protein motif:HMM:NF024750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HYT47575.1"
                     /translation="MHRLSTKTVEKAKEPGYYCDGGGLYLQVSPTASKSWIFRYTRHA
                     RSHEMGLGSERDVSLAEARTKASDARRQLVGGTRST"
BASE COUNT          671 a         1066 c         1136 g          581 t
ORIGIN      
        1 gagcggcacc tcgacctgat ggacgtgctg tcgatctacg gcggccgcgg cgcgccgctc
       61 gacgagatct cgcgcctggc cggctttccg ggcaaggtcg gcataggcgg cgacgcagtc
      121 tgggagacct accgccgcgg cgcgatcgcc gcgatccgcg actactgcga agccgactgc
      181 gcgaacacct acctgctttt cctgcgcttc cagctgatgc gctgcgcgct gaccaaagag
      241 cagtacgctc gcgaatgcag ccttctcaaa tcgttcatcg aaaaacagaa ccggccgcat
      301 tgggcagaat tcctgtcgct ctggaagaac tgatcctcgg catcgagtca ctggacgccg
      361 aggggcgcgg cgtcgcgcgc aaacccgatg gcaaggcggt gttcgtcgaa ggcgccctgc
      421 ctggcgagcg cgtgcaggcg ggcttagtcg cgagcaagcg ttcgttcgat gtttttaaaa
      481 gcatttctgt tcttcaaccc tcttccggtc gtcgcatcgc gcgctgcccg catttcggcg
      541 tctgcggcgg ctgcgccacg cagcacgcgg atgcgcctac gcagatggcg gcaaagcagc
      601 gctggctcga ggacagcctg cagcgcatcg gcaaagtggc gccggagacg gtgctgccga
      661 tcatctacgg cgaggagtgg ggttaccggc atcgcgcgcg gctctcggtg cgccgcgtgg
      721 cgaagaaagg cggggtgatg gtcgggttcc gcgagcggcg ctcgactcat gtggccgaca
      781 tgcgcgagtg cccggtcctg cctgcgcagg tttcggcgct catcccggcg ctcaagcagc
      841 tggtggagaa gctctcggtg cgcgagcggc tgccgcagat cgagctggca gccggcgaca
      901 acgccacggc gctggtattc aggcatctcc tgcctctcga agcagccgac caggtttttc
      961 tgcaggaatt ttctgaaaga cacggcgtac atgtctggct gcaaccgggc gggccggaga
     1021 cggcgcatgc gtttcatccc gtggcgagcg agctctacta cgaactgccg gagttcggcc
     1081 tgcgcatcca tttccgcccc accgaattca cccaggtcaa cccggcgctc aaccgcatcc
     1141 tggtgtcgcg cgcgctgcgc ctgctcgagc cgcagcccgg cgagcgcgtc gccgatctgt
     1201 tctgcgggct gggcaatttc tcgctgccga tcgcaagccg cggcgccgac gtggtgggct
     1261 tcgagggcag cgccgagctg gtggagcggg cgagggccaa tgcgtcggcg aacgggctgg
     1321 tcgcgcaatt ccaggtcacg gatctcttca agcacggcat cgctgctttc ggacctttcg
     1381 ataaattgct gttcgatccg ccgcgcgaag gcgctgtcga gctcatcaag tccctgcctg
     1441 aaggctggcc gcgccgcatc gtctacgttt cctgcgatcc tgcgaccctc gcgcgcgacg
     1501 ccggcgtgct ggcgcatacg aaaggcttcc gcctcgccgc ggccggcgtg gcgaacatgt
     1561 ttccgcacac cgcgcacgtc gagagcattg cactattcga acggggggaa tcgccatgaa
     1621 cgcgaagcat gcggtgttcg cgatggcggc tgcgttgctt gctgcatcgg cgttcgcggc
     1681 gccgaaaatc gatagcatcg tagtgaagcc caatcctgcc gccttcgcgg gcggcaaggc
     1741 gcccgaagtc gagatatcgg tgacggtgag ccgcacccgc ttcgacagcg gagcctgcga
     1801 cgctcgggtc gagttcggcg atggcgaagg ccggagcgtc gatttcggtg tcgccaccac
     1861 ccgcaccgtg cgccacgtct acaagaaagg cggcaattac cgcgtggtgg ccaggggagc
     1921 cggcaagaac ccctgcgagg gcacgcgcga cctcgcgttc aagctgaccg gcggacctgc
     1981 ggagaagaag gccgtgaaga agacagcggc gaagaaggac gccaagaaga aaggcgaaaa
     2041 gaaaaaggac gagaagaaca aagacgagcc caaataaaaa gggcgccgca gcgccctttt
     2101 ccgggtttag cttgaattaa tccctatccc ccgatagggc gagaaggatg ttcagcaggt
     2161 tggcgaagat gttgaacagg ctcatgtaca cgccggtcgt cgcaatgacg tagttggtct
     2221 cgccgccgcg gatgatgcgg ctcaggtcgt agagcaggaa cagcgagaac accacgatca
     2281 ccagcgtcga gatcgtcagc gcgagcgccg ggatctgcag gaacatgttg gcgaacatcg
     2341 cgatgagcag cgcgatcatg ccgacgaaca ggaacttgcc catgaagctg aagtcgcgct
     2401 tggtggtcgt ggcgaccacg cccatggcga agaagatcac gccggtgcct accgccgcgt
     2461 agcccacgag ctgcatgccg ttgcgcatgg cgagggcgta gttgaggatc ggcccgagcc
     2521 accagccgag caaccctgtc atcagcagca gcaacccgat gccgatggcg ctgttgcggt
     2581 tcgcggcgat gccgaattgc aggccgatca ccaccgccag catcaccagc gagctgatga
     2641 tcgggctcgc catgatcacc ccggcggtcg ccatgccgat gaaggcgccg atcaccgtcg
     2701 gcaccatcgt caacgcgagc agcaggtagg tgttgcgcag gaccttctgt ccctgcgcct
     2761 ggagcgcaac ctcttgcgcg tcgtaggtcg tcccgcgtcc gaacggctga tcgccggtgc
     2821 gcacgggttt cagttccggt tccatctcac cctccttcgt tatgctgata agactaccaa
     2881 agctgcaccg ggttcaacag cttacacctt ccacaaaggc taaaatgcgg gcgcaacagg
     2941 cttttttcaa gaggagagtt ggcagagtgg tcgaatgcgg cggtcttgaa aaccgttagc
     3001 ccgcaagggc tcgggggttc gaatccctca ctctccgcca gaacctgtcc cagcaagtcc
     3061 caagaagttc cagggggatt cgaacttggc aaatgaggat tcgaagtgag ccgcggggtg
     3121 cacaggcttt caacgaagac agtcgaaaag gcaaaggagc cgggctatta ctgcgatggc
     3181 ggcgggctgt atctgcaagt aagcccgacg gcgagcaaga gctggatctt ccggtacacg
     3241 cggcacgcaa gatcgcacga aatgggcctt ggatcggagc gcgatgtgag ccttgccgag
     3301 gctcgcacga aggcctcgga cgcgcgccgg cagctagtgg gcggcacgag atcgacctga
     3361 agaaggcggt ctggagcatc ccggccggcc gcatgaaagc gggcaaggag catcgcgtac
     3421 cgctctcgcc gcaggcaatc aagatcattg aggc
//
LOCUS       DASPXW010000173         5342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1437896, whole genome shotgun sequence.
ACCESSION   DASPXW010000173 DASPXW010000000
VERSION     DASPXW010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5342
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1437896"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VEL04_04085"
     CDS             complement(<1..201)
                     /locus_tag="VEL04_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47576.1"
                     /translation="MSRPHRVAIADDSPAFLAAAASHVSGLPGFMVAGTASSARQALA
                     LVESAMPDVLLLDLGLAPKRALD"
     gene            complement(301..945)
                     /locus_tag="VEL04_04090"
     CDS             complement(301..945)
                     /locus_tag="VEL04_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYT47577.1"
                     /translation="MIRVLLADDHSLVRAGIRSLLGAMADVQVVAEAASGEEALELAA
                     RQQPDVVLMDIAMKGITGLEAAARMRETQPAVRVVILSMHSGEEYVLQALRAGAAGYL
                     LKDAATGELELALRSVMRGESWLSPAVSRQVVEGYVQRSGAEAAPDVLTARQREVLRL
                     VAGGKSTKEIAFFLNLSVKTVETHRAQIMERLGIRDVPGLVRYALRTGLVPPDG"
     gene            complement(942..2039)
                     /locus_tag="VEL04_04095"
     CDS             complement(942..2039)
                     /locus_tag="VEL04_04095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HYT47578.1"
                     /translation="MTPLRFVAAALQRMVDKIDPQAGRYRMLFESSPLPMWVLDDQTL
                     RFLAVNDAAVRLYGYTREEFLAMTALDIDKEISAVSRGVFHHRKKGGEPVDVDGVGHL
                     VSWRGRPARLVFISDITERQRTQQALERLNRELEHSHERLRGLSRKLLEVQEEERGRL
                     ARDLHDDIGQALTALKIQLESLPRPGAEAALRTRIDECVETTRHTLERVRQLSLSLRP
                     PQLDDLGLAAALRSHLDRQARVAGLASHFDSLEAPQEIAPETETACFRVAQEAINNVL
                     RHARARNLWLRLSMAGGRLALSVRDDGRGFDVESVRRRDRVAASLGLVGMEERTALVG
                     GSFELRSTLGQGTVMLATFPLARADNQRAAL"
     gene            complement(2054..2129)
                     /locus_tag="VEL04_04100"
     tRNA            complement(2054..2129)
                     /locus_tag="VEL04_04100"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2094..2096),aa:Ala,seq:cgc)
     gene            2186..2932
                     /locus_tag="VEL04_04105"
     CDS             2186..2932
                     /locus_tag="VEL04_04105"
                     /inference="COORDINATES: protein motif:HMM:NF018928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapC family protein"
                     /protein_id="HYT47579.1"
                     /translation="MQISPPFGYKEVVPYLKNQKVRLLAPGQVPEFAQHGNAIPISQT
                     EFQPIARDYPIVFTSTDGKAFSAVAVLGMTAGENLFFADGTWVRGVYIPAYARRYPFC
                     MAKVNLNQVEQKDRLICVEKSFVDDEGEAMFDVEGKPNSKWQILERLLAEYEGDLERS
                     REMCAILSDYGLLEPFTMQAKFNPDKGGAAMQMTGMYRVAEKNLENLNAAQLKNLMRK
                     GMLPRIYIHLLSHENFGRLLERKGARQQAS"
     gene            3037..3696
                     /locus_tag="VEL04_04110"
     CDS             3037..3696
                     /locus_tag="VEL04_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47580.1"
                     /translation="MITGLAGLPILGFAAGALADTSLGIRAGTLGAGVELSHAFGPHT
                     GVRLNGDAFSRSTSRTEDNIQYDAKLRLRTASLLGDWFPFGNGFRISAGGVANGNKIN
                     LKGKPTGSTYTINGTTYNASDVGSLEGQVDFNKLAPYAGLGYGRALNKGLSLIFDAGV
                     MRQGSPKSKITVICGPAIVGTAQCTQLQNDAAAEQARLDDSLKKYKNYPVLSLGLAYT
                     F"
     gene            3843..4460
                     /locus_tag="VEL04_04115"
     CDS             3843..4460
                     /locus_tag="VEL04_04115"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYT47581.1"
                     /translation="MDRAFATVVEVGCSRGALARAYRQIFPQARYIGIEIDPDNAQTA
                     RQYCNDVICGDVENLGAKQWESLFPSDCWVFGDALEHLRDPWAVLRGVHQSIGAAGQV
                     LACIPNAQHWSVQARLASGIFRYEDQGLLDRTHLRWFTRITIIELFQSTGFRILEMFP
                     RIFDEPMKDRFLPGIISLATAAGGDRDVALRDSLPLQYVLRAVPI"
     gene            4506..5180
                     /locus_tag="VEL04_04120"
     CDS             4506..5180
                     /locus_tag="VEL04_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47582.1"
                     /translation="MIEVISATRLSESDFWNKSALGFSLRRLAHDARLVPHLAFANRR
                     GLPDIFNARISAPDGHELLVFVHDDVWIDDYFLADRVIEGLQTYDVIGVAGNRRRIQN
                     QPAWIFVDNQCTWDSATNLSGRVAHGRDPCGQVSFYGAVPAECELLDGVFLGARKSAL
                     TANGVLFDPRFDFHFYDMDFCRSARQNGLRLGTWPICLTHQSGGAFGKPPWIEKYRQY
                     VEKWKG"
     gene            complement(5183..5256)
                     /locus_tag="VEL04_04125"
     tRNA            complement(5183..5256)
                     /locus_tag="VEL04_04125"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5220..5222),aa:Ile,seq:cat)
BASE COUNT         1003 a         1705 c         1666 g          968 t
ORIGIN      
        1 gtcgagcgcg cgcttgggcg cgaggccgag gtcgagcagt agcacgtcgg gcatcgccga
       61 ctccaccagc gcgagcgcct ggcgcgcgct gctcgccgtt cctgcaacca tgaatccagg
      121 cagaccggag acgtgactcg ccgccgccgc aagaaacgca gggctgtcgt cggcgatcgc
      181 cacgcgatgc ggccgtgaca tttctctcat gagccgagag taaagactgc ctgccgccgc
      241 gaccatcggg ggcttcccta gccgggactt agggaaaacc tgagaacagg taaaaagtcg
      301 tcaaccgtcg ggcgggacga gcccggtgcg caatgcatag cgcaccaggc ctggaacgtc
      361 ccggatgccg aggcgctcca tgatctgcgc gcgatgcgtc tcgacggtct tcaccgaaag
      421 gttgaggaaa aaagcgattt ccttggtgct cttgccgccc gcgaccagcc tcagcacttc
      481 gcgctggcgc gcggtgagca cgtcgggcgc ggcttctgcg cccgagcgct gcacgtagcc
      541 ttcgacgact tgccgcgaaa cagcggggct gagccagctc tcgccgcgca tcacgctgcg
      601 cagcgcaagc tcgagctcgc cggtggccgc gtccttgaga aggtagccag ccgcgccggc
      661 gcgcagcgcc tgcagcacgt attcctcgcc cgagtgcatc gacaggatca cgacccgtac
      721 cgcaggctgg gtctcgcgca tgcgcgcggc cgcctcgagg cccgtgatgc ctttcatcgc
      781 gatgtccatc agcacgacat cgggctgctg ccgggccgcg agctcgagcg cctcctcgcc
      841 gctcgccgct tccgcgacca cttgcacatc cgccatggcg ccgaggagcg agcggatccc
      901 ggcgcgcacc agcgagtggt cgtcggcgag cagcacgcga atcataacgc cgcccgctga
      961 ttgtcggccc gcgcgagcgg gaaggtcgcg agcatcaccg tcccttgccc gagcgtcgag
     1021 cgcagctcga acgagccgcc gacgagcgcg gtgcgctctt ccatgccgac cagcccgagg
     1081 ctcgcggcca cgcggtcccg ccggcggacc gactcgacgt cgaacccgcg gccgtcgtcg
     1141 cgcaccgaga gcgcgagccg tcctcccgcc atggacagcc gcagccacaa attgcgcgcc
     1201 cgcgcatgac gcagcacgtt gttgatcgcc tcctgcgcca cgcgaaagca cgcggtttcg
     1261 gtttccggcg cgatctcctg gggcgcctcg agcgaatcga agtgcgacgc gagcccggcc
     1321 accctcgcct ggcggtcgag gtgcgagcgc agcgccgcgg cgaggccgag gtcgtcgagc
     1381 tgcggcggcc gcaggctgag cgaaagctga cgcacgcgct ccagcgtgtg gcgcgtggtt
     1441 tcgacgcatt cgtcgatgcg ggtgcgcagc gccgcctccg ccccgggccg cggcagcgat
     1501 tcgagctgga tcttgagagc ggtgagcgcc tgcccgatgt cgtcgtgcag gtcgcgcgcc
     1561 aggcgcccgc gctcctcctc ctggacttcg agcagcttgc gagaaagccc gcgcagccgc
     1621 tcatgggaat gctcgagctc gcgattgagc cgctcgagcg cctgctgcgt gcgctggcgc
     1681 tcggtgatgt cgctgatgaa taccagccgc gccggcctgc cgcgccagct gaccaggtgc
     1741 cccacgccgt cgacgtcgac aggctcgccg cccttcttcc tgtgatgaaa gacaccgcgg
     1801 ctcaccgcgc tgatttcctt gtcgatgtcc agcgcggtca tggcgagaaa ctcttctctg
     1861 gtatagccgt agaggcgcac cgccgcgtcg ttgacggcga gaaagcgcaa cgtctggtcg
     1921 tcgagcaccc acatcggcag cgggctgctc tcgaagagca tccggtagcg gccggcctgg
     1981 gggtcgatct tgtcgaccat gcgctgcagc gcggcggcga cgaagcggag cggagtcacg
     2041 ccaagggaaa tcgtggtgga gtggaggagg atcgaactcc cgaccttcgc attgcgaacg
     2101 cgacgctctc ccagctgagc taccaccccg cgaaggggtg tgattttaca gtacagtaac
     2161 atcaaagcct acggagcccc ctgccatgca gatttccccg ccgttcggct acaaggaagt
     2221 cgtcccgtac ctgaagaacc agaaggtgcg cctgctcgcg ccggggcagg ttccggaatt
     2281 cgcgcagcat ggaaacgcca tcccgatcag ccagaccgag ttccagccga tcgcgcgcga
     2341 ctacccgatc gtattcacct cgacggacgg caaggcattc agcgcggtcg cggtgctcgg
     2401 catgaccgcg ggcgagaatc tttttttcgc cgacgggacg tgggtgcgcg gcgtgtacat
     2461 tccggcgtac gcgcgccgct atcccttctg catggcgaag gtgaacctca accaggtgga
     2521 gcagaaggac cgcctgatct gcgtcgagaa atcgttcgtc gacgacgagg gcgaagccat
     2581 gttcgatgtc gaaggcaagc cgaacagcaa atggcagatc ctcgagcgct tgctcgccga
     2641 atacgaaggc gacctcgagc gcagccgcga gatgtgcgcg atcctctcgg actatggcct
     2701 gctcgagccc ttcaccatgc aggcgaaatt caaccccgat aagggcggcg cggcgatgca
     2761 gatgaccggc atgtaccgcg tcgccgagaa gaatctcgag aaccttaacg cggcccagct
     2821 caagaacctg atgcgcaagg gcatgctgcc gcgcatctac atccacctgc tgtcgcatga
     2881 gaatttcggc cgcctgctgg agcgcaaggg cgcgcgccag caagcgagct agcgttcgcg
     2941 gcccgcctcg gcacagcgtg tctggcgcgg aataggcggc ggaggaacat gttggcgaaa
     3001 gcgtgggaac atagggagag aacgatgctc cgccaattga tcaccgggct cgctgggctg
     3061 ccgatacttg gcttcgccgc tggagcgctt gctgatacgt cgctcggtat ccgtgccggc
     3121 acgctcggcg ccggcgtaga gctcagtcat gccttcggtc cgcataccgg cgtgcgcctg
     3181 aacggcgatg ccttcagccg cagcacctcg cgcaccgagg acaacattca atatgacgcc
     3241 aagctaagac tgcggacggc ctctttgctt ggagactggt tccctttcgg caacggcttt
     3301 cgcatcagcg ccggtggcgt ggcgaacggc aataagatca acctcaaggg caagccgaca
     3361 gggtccacgt acacgatcaa cggcacgacc tacaacgcat ctgatgtcgg ttccctggaa
     3421 ggacaagtcg acttcaacaa gctcgcaccg tatgccggcc tgggctacgg acgcgccctc
     3481 aacaagggcc tgtccctcat cttcgacgcg ggcgtcatgc gccagggaag cccgaagtcg
     3541 aagatcaccg tgatctgcgg gcctgcgatc gttggaaccg ctcagtgcac gcagttgcag
     3601 aacgacgccg cagccgagca ggccaggctt gacgacagcc tgaagaaata caagaactac
     3661 ccggtcctgt cgctggggct tgcctacact ttctagaggg tgcctgccgc ctaggcttgc
     3721 tcatcgcgca ggcgagctag cgttcgcggc ccgcctcggc agcgaccttc ttccaacttt
     3781 ctggcgataa catgagtcag actcccgctc acgaccatca caatccagat ctgctaaaac
     3841 tcatggaccg cgcctttgcc accgtcgttg aagtcggttg cagccgaggc gcgctcgcaa
     3901 gagcctatcg gcagattttc ccccaagccc gctacatcgg gatcgagatc gatccagaca
     3961 atgcccagac ggcgcgccaa tattgcaatg acgtcatttg cggcgatgtc gagaaccttg
     4021 gagcaaagca gtgggagtcg ctctttccga gtgattgctg ggtgtttggc gacgcgttgg
     4081 agcatctgcg cgatccttgg gccgtgctgc gcggcgtcca ccaatctata ggcgctgccg
     4141 gacaggtgct ggcctgtatc ccaaatgcac agcactggag tgtacaggcg cgacttgcat
     4201 ctggaatctt tcgttatgaa gatcaaggct tgcttgaccg tactcacctg cgctggttta
     4261 cgcgtatcac gatcattgaa ttgtttcagt ccaccgggtt taggattctc gaaatgttcc
     4321 cgcgcatatt tgatgaacca atgaaagatc gctttttgcc cgggatcatt tccttggcga
     4381 ccgctgcggg cggggaccga gacgtcgcgt tgcgcgattc cctgccgctt caatatgttc
     4441 taagagccgt gcccatttga tagaagtggg caccgatgca aacgtgaaat gctcaagatc
     4501 cgtccatgat tgaggtcatt agcgcgacaa ggttgtcaga aagcgacttc tggaataagt
     4561 ctgccctggg cttctcgttg cgacgccttg cacatgacgc tcgcttggtt cctcatctgg
     4621 cgttcgcaaa ccggcgcggc cttcccgata ttttcaacgc acgcatctcg gcaccggacg
     4681 gccatgagct gctcgtattc gtccatgacg atgtctggat tgacgactat tttcttgccg
     4741 atcgtgtgat cgaggggctg cagacctatg acgtgatcgg cgtcgccggc aatcgtcgca
     4801 ggatccaaaa ccaaccggct tggatatttg tggataacca gtgtacttgg gatagcgcaa
     4861 ccaacctcag cgggcgtgtc gctcacggaa gggatccctg cggtcaagtt tcgttttacg
     4921 gagcggttcc tgccgaatgc gagctcttgg acggtgtttt cctcggcgcc aggaaatcgg
     4981 cgttgacagc caatggagtg ctctttgatc cacgcttcga ctttcatttc tacgacatgg
     5041 atttttgccg cagcgcgcga caaaacggat tacgcctggg cacttggcca atctgcctca
     5101 cccatcaaag cggcggggcc tttggaaagc caccttggat cgaaaagtat cgccaatatg
     5161 tcgagaaatg gaagggctag cgtgggccgg gttggattcg aaccaacgac caagggatta
     5221 tgagtcccct gctctgaccg gctgagctac cggccccgcg cccttataac agaagggcta
     5281 gccttcctgg tccaggaagc ttttgaggcg ctcggagcgc gacgggtggc gcagcttgcg
     5341 ca
//
LOCUS       DASPXW010000174         2895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_302259, whole genome shotgun sequence.
ACCESSION   DASPXW010000174 DASPXW010000000
VERSION     DASPXW010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302259"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VEL04_04130"
     CDS             complement(<1..267)
                     /locus_tag="VEL04_04130"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HYT47583.1"
                     /translation="MKRAALIALFLSLATPVAAPAQALLDERQLLGMRLFNQSCRVCH
                     TKPQMASPQYAPLLSRASLGGNDGALRAFIANGSAKMPGFKYHFN"
     gene            complement(264..1904)
                     /locus_tag="VEL04_04135"
     CDS             complement(264..1904)
                     /locus_tag="VEL04_04135"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HYT47584.1"
                     /translation="MLKMCFLLLKVRFLLFVAAGLAFPAFAQLAGRVISPEGALEGVV
                     VSAKKAGSSVSISVVSDAAGRFRFPAAKLEPGNYALRIRATGYELESPLTAELADGAG
                     APLELKLRKAQDLSAQLTNAEWLASFPGTPDQKKFLYGCVGCHTLERVAKSKHDAAGF
                     RQVMKRMAGYANNSHIERPQVRVRARDPMRDFGPDADKQAVYLAAVNLSSGAWSYPLQ
                     PLPRVKGRSTRAVITEYELPRKPLMPHDVIVDGDGIVWFSQFDEQFLGRFDPATLKYT
                     EFPIPLQRPNFPKGTLDLEVDAEGNLWLSHMFQSGIVKFDKKTRRFQAWPLPPGVVND
                     DSQQSMVGPQRWTVDRKVWLNDAGIPGLHRLDLATGKFETWKPYANMKGPHSVYGIYA
                     DSQNNIFFMDFGGENVGKIEASTGKLTLYPTPTPRSRPRRGRMDGEDRVWFAEWRAEK
                     IGMFDTRTGKFREWPVPTPYTAPYDVVLDKTGKVWTAGMNTDRVLRMDLESGEFVEYP
                     LPSQTNIRRVFVDNSTMPPTFWVGNNHHATLVKVEPLE"
     gene            complement(1974..>2895)
                     /locus_tag="VEL04_04140"
     CDS             complement(1974..>2895)
                     /locus_tag="VEL04_04140"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYT47585.1"
                     /translation="AQEARINRFRQFSVPIRGIELHFIHEQGRAANPMPLLLSHGWPG
                     SIVEFLELIPLLTEHFTVVAPSLPGFTLSFKPGQPRFGVEEIADCFAELMTGVLGYER
                     FGAQGGDWGGFISSVLGLKYPQRLTGIHLNLLAVRRDPQIPSSTPEEAKYLKELSEWL
                     KEETGYQWIQGTRPQTLAYGLTDSPAGLAAWIAEKFHKWSDEDVRRDDLLANISLYWF
                     TAAIGSSFWPYHARMHGPWPIPAGTTVDVPTGYAEFPKEILRPPRSLASQTYRDIRRW
                     TVMQKGGHFAALEQPVALAREIDAFFLGVA"
BASE COUNT          501 a          909 c          974 g          511 t
ORIGIN      
        1 gttgaagtgg tatttgaaac cgggcatctt ggcgctgccg ttggcgatga acgcgcgcag
       61 cgcgccgtcg ttgccgccga gcgaggcccg cgacagcagc ggcgcgtact gcgggctcgc
      121 catctgcggc ttggtgtggc agacgcggca ggactggttg aagagccgca tgccgagaag
      181 ctgccgctcg tcgaggagag cctgcgccgg cgcggcgacg ggtgttgcaa gcgacaaaaa
      241 cagggcgatc aaagcggcgc gtttcattcg agcggctcga ccttgaccag cgttgcatgg
      301 tggttgttgc cgacccagaa ggtgggcggc atggtggagt tgtcgacgaa aacgcggcgg
      361 atgttggtct ggctgggaag cggatactcg acgaattcgc cgctctcgag gtccatgcgc
      421 aggacgcggt cggtgttcat gcccgcggtc cacaccttcc ccgtcttgtc gaggacgaca
      481 tcgtaggggg cggtgtaagg cgtcggcacc ggccattcgc ggaatttccc ggtcctggtg
      541 tcgaacatgc cgattttttc ggcgcgccat tcagcgaacc agacgcggtc ctcgccgtcc
      601 atcctgccgc ggcgcggccg cgagcgcggc gtgggcgtcg gatagagcgt gagcttgccg
      661 gtgcttgcct cgatcttgcc gacgttctcg ccgccgaagt ccatgaagaa gatgttgttc
      721 tgcgaatcgg cgtagatgcc gtacaccgag tgcggcccct tcatgttcgc gtagggcttc
      781 caggtctcga acttgcccgt ggccaggtcg agccggtgca gcccggggat gccggcgtcg
      841 ttcagccaca ccttgcggtc cacggtccag cgctgcgggc cgaccatcga ctgctgcgaa
      901 tcgtcgttga ccacgcccgg cggcagcggc caggcctgga acctccgcgt cttcttgtcg
      961 aacttcacga tacccgactg gaacatgtgc gagagccaca gattgccttc cgcgtcgacc
     1021 tcgagatcga gcgtgccctt ggggaaattc ggccgctgca gcgggatcgg aaactcggtg
     1081 tacttcaaag tggccggatc gaagcggccg aggaactgct cgtcgaactg cgagaaccag
     1141 acgatgccgt cgccatcgac gatgacgtcg tgcggcatca atggctttct cggcagctcg
     1201 tactcggtga tgacggcgcg cgtgctcctg cccttgacgc gcggcagcgg ctggagcgga
     1261 tacgaccacg cgcccgagct caggttgacg gcagcgagat acaccgcctg cttgtcggcg
     1321 tcggggccga agtcgcgcat cggatcgcgc gcgcgcacgc gcacctgcgg ccgctcgatg
     1381 tggctgttgt tggcgtagcc cgccatgcgc ttcattacct gccggaaccc ggcggcgtcg
     1441 tgcttcgatt tcgccacccg ctccagcgtg tggcagccga cgcagccgta gagaaacttc
     1501 ttctggtctg gcgttcccgg gaagctcgcc agccattcgg cgttggtgag ctgggcgctc
     1561 aagtcctgcg ccttgcgcag cttgagctcg agcggagcgc ccgcgccgtc ggccagctcg
     1621 gcggtgagcg ggctttcgag ctcgtagccc gtggcgcgga tgcgaagcgc atagttgccg
     1681 ggctcgagct tcgccgccgg gaagcggaaa cgtcccgcgg cgtcgctcac cacgctgatg
     1741 ctgacgctgg agcccgcttt cttcgcgctc accaccacgc cttccagcgc gccttccggc
     1801 gaaatcaccc gccccgcaag ctgcgcaaac gccgggaagg cgaggccggc ggctacgaac
     1861 agcaaaaagc gcaccttcag gagcagaaag cacatcttca gcatggctca ctcctttgat
     1921 cgcgaagcga tcctatactg gcgcggcgac ggagaaaacc atggaatcgc tggtcatgca
     1981 accccgagga agaaggcatc gatctcgcgc gccagcgcca cgggttgctc gagcgcggcg
     2041 aagtgcccgc ctttctgcat caccgtccag cggcggatat ctctataggt ctgcgaagcc
     2101 agggaccgcg gcggccgcag aatctctttc gggaattcgg catagcccgt cggcacgtcg
     2161 accgtcgtgc ctgcggggat cggccagggg ccgtgcatcc gcgcgtggta aggccagaac
     2221 gaggagccga tcgccgcagt aaaccagtaa agcgagatat tcgccagcag gtcatcgcgg
     2281 cggacgtctt catcgctcca cttgtggaat ttctcggcaa tccacgccgc gaggcccgcg
     2341 ggggaatcgg tgaggccgta ggcgagggtc tgcggcctgg tgccctggat ccactggtag
     2401 ccggtctctt ccttcagcca ttcggaaagt tctttcagat atttcgcttc ttccggggtc
     2461 gaagaaggaa tttggggatc ccggcgcacg gcgagcagat tgagatggat gccggtgagg
     2521 cgctgcggat atttcagccc tagtacagag gaaataaagc cgccccaatc acccccctgc
     2581 gcgccgaagc gctcgtaccc cagcacgccg gtcatcagct cggcgaagca gtccgcgatc
     2641 tcctcgacgc cgaagcgcgg ctggcccggc ttgaaggaaa gcgtgaaacc gggaagcgaa
     2701 ggcgcgacca cggtgaagtg ctcggtaagc agcgggatca gctcgaggaa ctcgacgatc
     2761 gagcccggcc agccgtgcga gagcagcagc ggcatcgggt tcgctgccct gccctgctcg
     2821 tggatgaaat gcagctcgat gccgcggatc ggcacgctga actggcggaa gcggttgatg
     2881 cgcgcctctt gggca
//
LOCUS       DASPXW010000175         2288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_302282, whole genome shotgun sequence.
ACCESSION   DASPXW010000175 DASPXW010000000
VERSION     DASPXW010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302282"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..561
                     /locus_tag="VEL04_04145"
     CDS             154..561
                     /locus_tag="VEL04_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYT47586.1"
                     /translation="MNRIVHLALKVDDLERSTEFYRKVFGFAEVNTEKVRDHTSRHLT
                     DGVIDVALIKYDAGTQSAESKAAGAGPCIHHFAVEVENMRTAEAEIRRLGCEIISDPG
                     VVPVKFRAPGGTVCEIVPKGRYKKLTREKAKKR"
     gene            564..992
                     /locus_tag="VEL04_04150"
     CDS             564..992
                     /locus_tag="VEL04_04150"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HYT47587.1"
                     /translation="MSSDFWSGLALGALGGYIIATASGWEYLGPEGPGPGFFPLWYGI
                     AMLVLSGMLVITNARKKVIIEKCVDRTEIIRALGTWLAFAVSIAALKLVGFVIGFAAL
                     TFFIVAVMYRRRLRVAASVALAGAAAFYVLFPLALGVPLP"
     gene            994..>2288
                     /locus_tag="VEL04_04155"
     CDS             994..>2288
                     /locus_tag="VEL04_04155"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HYT47588.1"
                     /translation="MEILSGLMQGFAVALQPANLLWCFVGVVLGTVVGIMPGLGPPAT
                     IAMLLPLTFLMHPAGAMIMLAGIYYGAKYGGSTTSILLNVPGESASVVTCLDGYQMAR
                     KGRAGPALGIAAIASFIAGTVGVLGLMLVAPPLAKMALAFSSPEYFALMALGLAMVVL
                     LAGHSMVKALLAMLVGLWIAGIGTDLFTSTSRFTFGRMELLSGIDFVVVAIGIFAIGE
                     VLANVEAREEAQLLPVPKGLRNLLPTVQDLKDCRFAFVNGSVIGFLVGVLPGAGSTIA
                     SFISYGVEKAVSRRRSEFGTGVIEGVAAPEGANNSETGGALVPLLTLGIPGSGTTAVL
                     LAALVLWGFKPGPLFIPENPALFWGLVASMYIGNMMLLVLNLPLVPLFAQVLRAPVYV
                     LYPLILGVSMVGVYSASGSLFDLGLLAVFGLAGYLMRKL"
BASE COUNT          336 a          735 c          764 g          453 t
ORIGIN      
        1 gtgccgaagt ggatgcgaaa ggttacgatc tgatggcgcc ggtgatcggt aaagtccgcg
       61 cgcgccgcct ctgcgacgcc ttgtggaatt tggaacacat taaagacgtt cgccggctac
      121 gttcattatt gcgaaattag aactggagaa gccatgaacc gcatcgtcca tctggcgctg
      181 aaggtcgatg acctcgagcg cagcaccgag ttctatcgca aggtgttcgg cttcgccgag
      241 gtcaacaccg agaaggtgcg cgaccacacc tcgcgccacc tcaccgacgg cgtgatcgac
      301 gtcgcgctga tcaagtatga cgccggcacg cagtccgcgg agtcgaaggc ggcgggagcg
      361 gggccgtgca tccaccactt cgcggtcgag gtcgaaaaca tgagaaccgc cgaggccgag
      421 atccgcaggc tcggctgcga gatcatcagc gaccccggcg tggtgccggt caagttccgc
      481 gccccaggag gcaccgtctg cgagatcgtg ccgaagggac gctataaaaa actcaccagg
      541 gaaaaagcga agaagagata gccgtgtcct cggacttctg gtccgggctc gcgctcggcg
      601 cgctcggcgg gtacatcatc gcgaccgcgt cgggctggga atacctcggc ccggaggggc
      661 cgggccccgg gttctttccg ctgtggtacg gcatcgcgat gctggtgctt tccggcatgt
      721 tggtcatcac taatgcccga aagaaggtca ttattgaaaa atgcgttgac cggacggaaa
      781 tcatccgcgc gcttggcacc tggctcgcgt tcgccgtatc gatagcagcg ttgaaactcg
      841 tcggcttcgt catcggcttc gctgcgctga cctttttcat cgttgcggtg atgtatcgcc
      901 ggcggttacg ggtcgctgcc agcgtggccc tcgccggcgc cgccgcgttc tacgtgcttt
      961 tcccgctggc gctcggcgtg cctcttccct agcgtggaaa tcctttccgg cctgatgcag
     1021 gggttcgcgg tggcgctgca gccggccaac ctgctgtggt gcttcgtcgg cgtggtgctc
     1081 ggcaccgtgg tggggatcat gcccggcctc ggaccgccgg cgaccatcgc catgctgctg
     1141 ccgctcactt tcctgatgca tccggcgggc gccatgatca tgctcgccgg catctactac
     1201 ggcgccaagt acggcggctc caccacctcg atcctgctca acgtgcctgg cgagtcggcc
     1261 tcggtggtga cctgcctcga cggctaccag atggcgcgca aggggcgcgc gggtcccgcg
     1321 ctcggcatcg ccgcgatcgc ttccttcatt gccggcaccg tgggcgtgct cggcctgatg
     1381 ctggtcgcgc cgccgctcgc aaagatggcg ctcgccttca gctcgccgga gtatttcgcc
     1441 ctgatggcgc tggggctcgc gatggtggtg ctgcttgccg ggcactcgat ggtgaaggcg
     1501 ctgctcgcca tgctggtcgg cctgtggatc gcaggcatcg gcaccgatct cttcaccagc
     1561 acctcgcgct tcacgttcgg tcgcatggag ctcctgtcgg gcatcgactt cgtggtcgtc
     1621 gcgatcggca tcttcgccat cggcgaggtg ctcgccaacg tggaggcgcg cgaggaggcg
     1681 cagctcctgc cggtgcccaa ggggctgcgc aacctgctgc cgacggtgca ggatctcaag
     1741 gattgccgct tcgccttcgt caacggttcg gtgatcggct tcctggtcgg agtcttgccg
     1801 ggcgccggct cgaccatcgc ctcgttcatc tcctacggcg tggaaaaggc cgtgtcacgg
     1861 cgccgctccg agttcggcac cggcgtcatc gaaggcgtgg ccgcgcccga gggcgcgaac
     1921 aattccgaaa ccggcggcgc gctggtgccg ctgctcacgc tgggcattcc cgggtccggg
     1981 accaccgcgg tgctgctcgc cgcgctggtg ctgtggggat tcaagcccgg gccgctcttc
     2041 atcccggaga atccggcgct gttctgggga ctggtggcga gcatgtacat cggcaacatg
     2101 atgctgctgg tgctgaacct gccgctggtg ccgctgttcg cgcaggtgct gcgcgcgccg
     2161 gtgtatgtcc tgtacccgct gatcctcggc gtgtcgatgg tgggcgtcta cagcgcctcg
     2221 ggcagcctgt tcgacctcgg gctgctggcc gtcttcgggc tcgccggtta cctgatgcgc
     2281 aagcttga
//
LOCUS       DASPXW010000176         2449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_806544, whole genome shotgun sequence.
ACCESSION   DASPXW010000176 DASPXW010000000
VERSION     DASPXW010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806544"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(375..821)
                     /locus_tag="VEL04_04160"
     CDS             complement(375..821)
                     /locus_tag="VEL04_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47589.1"
                     /translation="MTGKADFTPEEWQVVLEGPPSAGLIVVTAQRGGSFRETIAIAKA
                     YAEARQQHGESELLDEIVSAKPQTDHTRYHSVEELKEQGLQHLRDAVGLLQRKATPEE
                     LEDYKRFVLNLADRVANAHREGGESVSPAERAALEEIAASLDLPAP"
     gene            1279..1464
                     /locus_tag="VEL04_04165"
     CDS             1279..1464
                     /locus_tag="VEL04_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47590.1"
                     /translation="MESADFNGAFTQFVSSRDGFDLWFKERFADVSGIDLNDPPEMQL
                     PELLSAYEVGVPVEAQG"
     gene            complement(1883..>2449)
                     /locus_tag="VEL04_04170"
     CDS             complement(1883..>2449)
                     /locus_tag="VEL04_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47591.1"
                     /translation="EESLELRQKLGHRDLVTRSLLTICQLIVSRGDVDQAEPIAQDLY
                     RRAVDASDWAGELAALALLASCALIRGDFATAEQDYARAAVFAWEHGDRRECANQMQG
                     VAIAVSGRGDYERALRIFGATVAERERRGLEWNLPFWMQLLDEHLGRARAALGSEAAA
                     AAEAEGHGIPFERAVEEAVQAWSVAETT"
BASE COUNT          396 a          800 c          819 g          434 t
ORIGIN      
        1 taacggccga ccccgagacg gccgagcgct ggcgggccga gctcgccgcc gggaggctcc
       61 cacagaccag caagaacgag ccccaacagc ccgttcactt gtcgctgttc caacaaagaa
      121 cgacagcttt gagagggccg aggtctgaat ctacgtgcga ctggggtaca agcgacggcg
      181 ggaaccagtc cacgcgccga ccgatccccg cgcccgtcct gcgcatgccg gtcagcgccc
      241 gacacggaag gcaccggact cgaaccggaa ccgcgggcta cgcggtcatt cccccttccg
      301 ggcgtgcctt ccgggcgcga ttctaccttc ggtacagcga cgacatcgag gagtcaatgg
      361 ccagtagaag aggctcaagg agctggcaga tccaagctcg ctgcaatctc ctcgagcgcg
      421 gcgcgctcgg ccgggctgac gctctccccg ccctctctgt gagcgttggc gaccctgtcc
      481 gcgaggttga gcacgaaacg cttgtagtcc tcgagctcct ccggcgtcgc cttgcgctgg
      541 agcaggccaa cagcatcgcg aagatgttgc agaccctgtt ctttgagttc ctcgaccgag
      601 tggtagcggg tgtggtcggt ctggggcttc gcagacacga tctcgtcgag cagttcgctc
      661 tcaccgtgtt gctgccgcgc ctccgcatat gccttcgcta tcgcgatcgt ttcccggaag
      721 ctcccgccgc gttgcgccgt gacgacgatc aggcccgcgc tcggcggacc ctcgagcacg
      781 acctgccact cctcgggtgt gaagtcggcc tttccagtca tgccatcctt tcctctctgc
      841 tcgtagcaac gactaccgcc cgcggcacag tagccgctca aaccgaacgc acggcttgcc
      901 gttgtcgccg cttgttggaa aacgcgatgg agactcccgc ctagcgaggc gagtttggcg
      961 ccgtcaaggc ggaaccgaag gcgccctgcc acagccacgg tcggtccagg cgcttagccg
     1021 gatgtcgcct aagcagcgtt gacttggtcg gaccgctgtc ggagactctt tcgtcggatt
     1081 cggttgggag gaagccatgg atcaggtttg ccttgtagca ccggtacagg aaggaaaagg
     1141 ggaagcggcg cggtcgttta tgcgtgatct cgacggatcg cggcgggagg agtacgaccg
     1201 ctccgagcag cggatcggga tcaggaagga agtgtggttc ctcgcgccga cgcctgcggg
     1261 cgagctgcgg tcggctatat ggagagcgcc gacttcaatg gcgcgttcac gcagttcgtc
     1321 agctcacgcg atggctttga cttgtggttc aaggagcggt tcgccgatgt gagcggaatc
     1381 gacttgaacg atccgccgga gatgcagctt cctgagctgc tgtcggccta cgaagtgggc
     1441 gttcctgttg aggcgcaggg gtaaagttgc agacagacta ggtttgcgaa gcagttcgtt
     1501 cgctccattc gtttcgcctc ggggaacgag ccgcaggctc cgcgcgcagt aatcgcgggt
     1561 gcgcggccac aatgaagccg tcgcacttcc ggcaaagtgc gacagcgacg cgcttcgagc
     1621 gcgagctggt gggtcgcctg ccgactgact ccatctcacg gccggcgctc tgcggagccc
     1681 cttgggcgtg ggttcgcgct agcgtcgagg cgtgaacgag gttcctcgtc agcgaaattg
     1741 ctgcgggcag aggcgctgag cttcttctcg gcggaggagg tggcgcgggc acggacctac
     1801 cgccgtcctt tgtcctgggc agggctgctg acgttgcgat cgaggcgagc gtgttggccg
     1861 cgcttgtgtg gggcggccac cgctacgtcg tctcggcgac ggaccacgcc tggacggcct
     1921 cctcgaccgc acgctcgaag ggaatgccgt gcccctccgc ctcggcggcc gccgccgctt
     1981 ccgaccccaa tgcggcccgt gcgcggccga ggtgctcgtc cagcagctgc atccagaagg
     2041 ggagattcca ctcgaggccg cgccgttccc gttcggccac cgtcgcgcca aagatccgga
     2101 gcgcgcgttc gtagtcgcct cggccggaga cggcgatggc aacgccctgc atctggttcg
     2161 cgcattctct tcggtcgccg tgctcccagg cgaacaccgc ggcacgcgcg tagtcctgct
     2221 ccgcggtcgc aaagtcgccg cggatgagcg cgcagctggc gagcagggcg agcgcggcca
     2281 actcgcccgc ccaatctgag gcgtccacgg cgcgccggta gaggtcctgg gcgatcggtt
     2341 cggcctgatc gacatcgccg cggctgacga tcagctggca gatggtcaac agcgagcgtg
     2401 tcacgagatc gcggtggccc agcttctgcc gcagctcgag gctctcctc
//
LOCUS       DASPXW010000177         4145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1315714, whole genome shotgun sequence.
ACCESSION   DASPXW010000177 DASPXW010000000
VERSION     DASPXW010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1315714"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="VEL04_04175"
     CDS             <1..741
                     /locus_tag="VEL04_04175"
                     /inference="COORDINATES: protein motif:HMM:NF023334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon and DUF3426 domain-containing
                     protein"
                     /protein_id="HYT47592.1"
                     /translation="VCGTAFRVQREQLAARSGKVRCGKCGAVFDGVSALVEEGAEKLA
                     LEPSPQLGLFDPSRRGPPGAARPLEDTPLPAFMAEEEPKRRLGWMWGLAALAAAAALA
                     TQATYRYRAELAVLAPAARMPLDAACRLFRCEVPLPRRPDLMSIESSDLQADPRREGL
                     IVLNAVLRNRARFAQDYPALELTLTDEGDRPLVRRVLSPRDYLDSARLAQLVAQGIAP
                     GGEASLRVFLDSSRTRATGYRLYLFYPS"
     gene            738..1679
                     /locus_tag="VEL04_04180"
     CDS             738..1679
                     /locus_tag="VEL04_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HYT47593.1"
                     /translation="MRILVTGSIAYDTIMVFPDRFRNHLLADQLHILNVCFLTPEMRR
                     EYGGTAGNIGYNLKLLGEDPMVMATVGEDIGPYLERLNTLGIATRLLKRIPGQFTAQA
                     FITTDLDDNQITAFHPGAMNYSHENRLTRELGAGIAILAPDGKQGMLQHARECAEHNV
                     PFVFDPGQGLPMFSRAELDEFLRLADYVAVNDYEGKMLEEKTGRKLDELAREVKAFVV
                     TLGAQGSLLLAAGQRLEVPCVQADRVIDPTGCGDAYRAALLYGIAHGWDWMNAGKLAA
                     VMGAIKVAHRGAQNHKAGRDEIEMRFRREFGYSPWKG"
     gene            1684..2130
                     /locus_tag="VEL04_04185"
     CDS             1684..2130
                     /locus_tag="VEL04_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HYT47594.1"
                     /translation="MRSTLVMTLMAAALAGCATHESANVYDKHEAGREQLVRFATVDS
                     VRKVTIQGSQSGVGAAAGGVVGGVAGSGVGHGKGSTIVGVLGGVAGGVAGNTLEEKAT
                     SKNALEIIVKLDSGELRAIVQEADVLLTPGQRVRLITSGGITRVTP"
     gene            complement(2127..2540)
                     /locus_tag="VEL04_04190"
     CDS             complement(2127..2540)
                     /locus_tag="VEL04_04190"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HYT47595.1"
                     /translation="MSDCVFCKIVAKQIPATVVLEDEHTVAFMDLGQVNPGHVLVAAK
                     AHVENLYGLDDVNAAAVFRTVARVARAIRDAFAPQGLSVYQANGRPAGQTVFHFHVHL
                     VPRHEGDGMALTWPVKNPPRENLEHYATKIRAKLA"
     gene            complement(2537..3355)
                     /locus_tag="VEL04_04195"
     CDS             complement(2537..3355)
                     /locus_tag="VEL04_04195"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HYT47596.1"
                     /translation="MATYAIGDVQGCFDELKQLLNDFGYSSKRDRLWFVGDLVNRGPK
                     SLEVVRWVRNLGARAVTVLGNHDLHLVTQYEGFERRRKDDTFDDVLDAPDGGELVAWL
                     RTRPMMHADGRFAIVHAGLLPQWSIQKSLSLGKEVEAALAAPNYRNFLANMYGSTPDR
                     WSDSLAGWDRLRVIVNAMTRMRFCTRAGKMDFRAKGVAAPRGYVAWYETRPGTEKTAI
                     LCGHWSALGLKLSKRLAVLDTGCVWGGSLTALRLEDRALYQVPCRGYQTAGEER"
     gene            complement(3362..>4145)
                     /locus_tag="VEL04_04200"
     CDS             complement(3362..>4145)
                     /locus_tag="VEL04_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYT47597.1"
                     /translation="SEALEVAAVHSVSTQGFEAARWGERPYRAPHHGASAAALVGGGN
                     MPRPGEISLAHHGVLFLDELPEFDRDVLEALREPLESGRVSIARAARQAQFPARFQLV
                     AAMNPCPCGHCGDRSGRCRCTPERIARYRGRISGPLADRIDIKLEVPAPREAELVAPI
                     AGEPSSEIAKRVARARELQLARQGTPNALLGTRDIDRHCGTDRDGDQLLRHALARLLL
                     SARAYHRVLRVARSIADLAGKGTIAAEHIAEAIQYRRLDASF"
BASE COUNT          679 a         1424 c         1367 g          675 t
ORIGIN      
        1 gtgtgcggca cggcgtttcg cgtgcagcgc gagcagctcg ccgcgcgctc tggcaaggtg
       61 cgctgcggca agtgtggcgc ggtgttcgac ggcgtttccg cgctcgtgga ggagggcgcg
      121 gagaagcttg cgctcgagcc gtcgccgcag ttggggctgt tcgacccgtc gcggcgcggc
      181 ccacccggcg ccgcccggcc gctcgaggac acgccgctgc ccgccttcat ggcggaggag
      241 gaaccgaagc gcaggctggg ctggatgtgg ggcttggccg cgcttgccgc ggccgccgcg
      301 ctcgcgacac aggccaccta ccgctaccgc gcggagcttg cggtcctcgc gccggcggcg
      361 cgcatgccgc tcgacgccgc gtgccggttg ttccgctgcg aggtgccgct gccacgccgg
      421 cccgacctca tgagcattga atcgtccgac cttcaggccg atccccgccg cgaaggcctc
      481 atcgtgctga acgcggtgct gcgcaaccgg gcacgcttcg cgcaggatta tcctgcgctc
      541 gagctgaccc tcactgacga gggcgaccgc ccgttggtgc ggcgggtgct ctcgccgcgc
      601 gactatctcg attccgcgcg cctcgcgcag ctcgtcgccc aaggcatcgc gccgggcgga
      661 gaagcctcgc tgcgcgtgtt cctcgactcg agccgcaccc gtgccaccgg atatcgcctc
      721 tacctctttt atccgtcatg agaatcctcg tcaccggctc gatcgcgtac gacaccatca
      781 tggtgttccc ggaccggttt cgtaaccatt tgctcgccga ccagctgcat atactcaacg
      841 tctgtttcct cacgcccgag atgcgccggg aatacggcgg cacggccgga aacatcggtt
      901 acaacctgaa attgctcggc gaagacccca tggtgatggc tactgtcggc gaggacatcg
      961 gcccctacct cgagcgcttg aacacgctcg gcatcgctac gcggctgctg aagcgcattc
     1021 caggacagtt cacggcgcag gcattcatta ccaccgacct cgacgataac cagatcaccg
     1081 cctttcaccc cggcgcgatg aactactcgc acgaaaatcg cctcacgcgc gagctcgggg
     1141 cggggatcgc gatcctcgcc cccgacggca agcaggggat gctgcagcat gcgcgggaat
     1201 gcgccgagca caacgtgccc tttgtcttcg atcccggcca gggcttgccg atgttctcgc
     1261 gcgccgagct cgatgagttc ctgcgcctcg cggactacgt cgcggtgaac gactacgagg
     1321 gcaagatgct cgaggaaaaa accgggcgca agctcgatga gctggcgcgt gaggtcaagg
     1381 cgttcgtcgt cacgctcggc gcgcagggct ccctgttgct cgcggcaggt caacggctgg
     1441 aggtgccgtg cgttcaagcc gatagggtga tcgatccgac gggctgcggc gacgcctacc
     1501 gcgccgcgct gctatacggc atcgcccacg gctgggactg gatgaatgcg ggtaaacttg
     1561 cggcggtgat gggcgcaatc aaggtcgccc accgtggcgc gcagaatcac aaagccgggc
     1621 gcgacgaaat cgagatgcgg ttccggcgcg agttcggcta ctcgccctgg aaaggatgac
     1681 agcatgcgaa gcaccctggt gatgacgctg atggcggccg cgcttgccgg ctgcgccacg
     1741 cacgaatcgg caaacgtcta cgacaaacac gaagccggcc gcgagcaact cgtgcgcttt
     1801 gccaccgtgg actcggtgcg caaggtgacg atccagggca gccaatcggg cgtcggcgcg
     1861 gccgcgggcg gggtcgtcgg aggggtcgcc ggcagcggcg tcgggcacgg caagggctcg
     1921 accatcgtcg gcgtactggg cggcgtggcg ggcggcgtag cgggaaatac gctggaagag
     1981 aaggcgacca gcaaaaacgc gcttgaaatc atcgtcaagc tcgatagcgg cgagcttcgc
     2041 gccatcgtcc aggaagcgga cgttctgctc acccccggcc aacgcgtgcg gctaatcacc
     2101 tcgggcggca tcacccgcgt cacgccctag gcgagcttgg cgcggatttt cgtcgcgtaa
     2161 tgctcgaggt tttcgcgcgg cggattcttc accggccaag taagggccat gccgtctccc
     2221 tcgtggcgcg gcacgaggtg aacgtggaaa tgaaatacgg tctggccggc cggcctgccg
     2281 ttcgcctgat aaaccgataa gccctgcggc gcgaacgcgt cgcggatcgc acgcgcaacg
     2341 cgcgccacgg tgcgaaatac cgccgcggcg ttcacatcgt ccagcccata caggttctcg
     2401 acgtgagctt tggccgccac cagtacatgg cccgggttca cctgaccgag gtccatgaaa
     2461 gccaccgtgt gctcgtcttc gagcaccacg gttgccggaa tctgcttcgc cacgatcttg
     2521 caaaacacgc agtcgctcat cgttcctctc ctgctgtctg atagccgcgg cacggcacct
     2581 ggtagagcgc gcggtcttcg agcctgagtg cggtgagcga gccaccccag acgcagcccg
     2641 tgtcgagcac tgcaaggcgc ttgctcagct tcagcccaag cgcggaccaa tgaccgcaca
     2701 ggatcgctgt cttttcggtc ccgggccgcg tctcgtacca cgcgacatag ccgcgtggtg
     2761 ccgcgactcc cttcgcgcgg aaatccatct ttcctgcgcg cgtgcaaaag cgcatgcgcg
     2821 tcattgcatt cacgatcacg cgcagacggt cccagccggc gagcgaatcg ctccacctgt
     2881 cgggcgtgct gccgtacatg tttgccagga aattccggta attcggcgcc gcgagcgctg
     2941 cctcgacctc cttgccgagg ctcagcgatt tctgaatgct ccattgcggc aatagtcccg
     3001 cgtgtactat cgcgaagcgg ccatcggcgt gcatcatcgg ccgcgtgcgc agccacgcga
     3061 ccagttcgcc gccatccggc gcgtcgagca cgtcgtcgaa ggtatcgtcc ttgcgccggc
     3121 gctcgaagcc ttcgtattgc gtcaccagat gcaagtcgtg attgcccagc accgtgacgg
     3181 cgcgggcgcc gagattcctg acccagcgca ccacctcgag cgacttcggc ccgcggttga
     3241 ccagatcgcc cacgaaccac agccgatcac gcttcgagct gtatccgaaa tcgttcagca
     3301 actgcttcag ctcatcgaag cagccctgca catcgccgat tgcgtacgtc gccatattca
     3361 cctagaaact ggcatcgaga cgccggtact gtatcgcctc cgcgatgtgc tccgcagcga
     3421 tggtgccttt gccggccaaa tcagcaatcg agcgcgccac ccgcagcacg cggtgatagg
     3481 cgcgcgccga gagcagcagc cgcgccagcg cgtgccgcag cagctggtcg ccgtcgcggt
     3541 cagtgccgca gtggcggtcg atgtcgcgcg tgccgagaag cgcattcggc gtcccctggc
     3601 gcgcgagctg gagctcgcgc gcccgcgcga cgcgcttcgc gatttcgctc gaaggctcgc
     3661 ccgcgatcgg ggcgacgagc tccgcctcgc gcggcgccgg aacctcgagc ttgatgtcga
     3721 tgcggtcggc gagcgggccc gaaatccggc cccggtagcg cgcgatgcgc tcgggggtgc
     3781 agcggcagcg cccgctgcgg tcgccgcagt gtccgcaggg acacggattc atggccgcga
     3841 cgagctggaa gcgtgccgga aactgcgcct ggcgcgccgc gcgcgcgatc gatacgcgtc
     3901 cggactcgag cggctcgcgc agcgcctcga gcacgtcgcg gtcgaattcg ggcagctcgt
     3961 cgagaaacag cacgccgtgg tgcgcgagcg agatctcgcc ggggcgcggc atgttgccgc
     4021 cgccgaccag cgccgcggcg gaggcgccgt gatgcggcgc gcggtagggt cgctcgcccc
     4081 agcgcgcggc ctcgaagccc tgcgtcgaga ccgagtggac cgccgcgacc tccagcgcct
     4141 cgctc
//
LOCUS       DASPXW010000178         5156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1061276, whole genome shotgun sequence.
ACCESSION   DASPXW010000178 DASPXW010000000
VERSION     DASPXW010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5156
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1061276"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VEL04_04205"
     CDS             <1..299
                     /locus_tag="VEL04_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47598.1"
                     /translation="QFLLKKHPKGTTGSGAVDHIAFVASNPREVRSRIRKNRVEMHYR
                     SFPEAKLFQIFLKDPDDVTIELNFLGEAIDEKQWKGKGSASAVTLDGGKAKRQN"
     gene            301..1422
                     /locus_tag="VEL04_04210"
     CDS             301..1422
                     /locus_tag="VEL04_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HYT47599.1"
                     /translation="MLFEKIQIREIAIRNRIVVSPMCQYSAEDGHVTDWHFVHLGKFA
                     QGGAGVVFVEATAVEKRGRITHGDTGIWDDAHIAGLRKISGFIRSQGAVPAIQLAHAG
                     RKSSMARPWYGNGPLTQADIERGDKPWDIVAPTDRPIGDGWIKPRALGRDDLSDLLDA
                     YRAAARRALKAGFDILEIHAAHGYLLHTFLSPLSNDGSREDRMRFPLEVARAVREAWP
                     AQKPLFWRCSSIDDVEGGWSIEDTICLARQLRQGGVDVVDCSSGGILGSATAATRTLL
                     PRVPGFQIPFAEKVKKEAGVMTMAVGLILTPQQAEEAVQSGRADLVAIAREALYDPNW
                     PLHAAIALGVDPDFAQWPVQYGWWLTRREGLLRKLGVRR"
     gene            1419..1988
                     /locus_tag="VEL04_04215"
     CDS             1419..1988
                     /locus_tag="VEL04_04215"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYT47600.1"
                     /translation="MKTIRLSAAQMQQRIARFKSLAPLPIQMGEIPLAARDVVYSRKL
                     LSVIGLDEEASTPINANAPIRGAAGMTMTLAVCPPGQGPGLHSHRQTYETFTVLKGRF
                     EVSWNDDGGESAVLELFDTVSFPPGVCRAFRNVGKEEGILQVIITGGVHDMTDIDFSE
                     KAKREIEAIGPGLAKEFEKAGFSFTAGAA"
     gene            complement(2086..2451)
                     /locus_tag="VEL04_04220"
     CDS             complement(2086..2451)
                     /locus_tag="VEL04_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47601.1"
                     /translation="MPWRNATKVVAAAALASGCATADPDLAAARDSWQGARYDDVVRA
                     WGAPARSSKQDGRETHAWVSEEYGVIRGSGGMGGAGGIVFGPAGDPVRCDRTLVFSGG
                     KVVEQSWTGPVAYCNRFKK"
     gene            complement(2438..3343)
                     /locus_tag="VEL04_04225"
     CDS             complement(2438..3343)
                     /locus_tag="VEL04_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HYT47602.1"
                     /translation="MSYRILSYLAGREPKGGILVGEQIYDLTRSYPSVLAALQDWAKA
                     RRELSALAKKSAGKKGLPLKRAKLLAPILYPGNIYCAGANYTDHVAEMERAQGRTPGP
                     NMKQLGEKPWHFVKSSRSSIVGPGAAVKLPAYSSAVDWEIELAAVIGRKAKDVPVERA
                     LECIAGYTIANDLSARDAMKRAKNPPASPFHYDWVSQKCFDGACPLGPWIVPAAEIPD
                     PHSLGLKLWIDGELMQDSNTGKMIFDTAEQIAMLSSRVTLQPGDLILTGTPAGVGMGR
                     GRFLKPGEKLKLWIERIGELEHAVA"
     gene            3386..4372
                     /locus_tag="VEL04_04230"
     CDS             3386..4372
                     /locus_tag="VEL04_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYT47603.1"
                     /translation="MRAILAGLAFLLLGSCATPPVAPKTVNLIVFPGGFNWPIWVAQE
                     KGLFAKNGLDVKVTPTPSSVFQLTNLIGGKFDIAMTAIDNLIAYREGQGEEPVPGPDL
                     FAFMGGDNGFLRLVAVPDVKTHGDLKGKTVSVDARTTGYAFVLFEMLERGGLRLDQDY
                     KVERAGGVLQRFQALMEKKHAATLLLSPFELQAEAKGFNRLGNATDVLGAYQGLVGGA
                     RKSWADANREAVVGYIRAFSEAVDWLYDPRNKDEAIAVFRKNLPNVPAQGAQAAYGVL
                     LSPREGFQKKARIDMEGVRTVLRLRSKYAEPRKLLTDPAKYYDDSFYRAALR"
     gene            4407..4703
                     /locus_tag="VEL04_04235"
     CDS             4407..4703
                     /locus_tag="VEL04_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47604.1"
                     /translation="MRPDPALIDRVLPSERLMKQVIGGLSVFTLLMTIPQIVMIWASH
                     QAAGVSLLSWGAYWISAFVWFGYGLRKHDPNIYLPCIGWLLLDGAVIAGTVIYG"
     gene            complement(4700..5098)
                     /locus_tag="VEL04_04240"
     CDS             complement(4700..5098)
                     /locus_tag="VEL04_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding protein"
                     /protein_id="HYT47605.1"
                     /translation="MTGMTIGATARSASVSVETIRYYQRLGLMPKPARARGSVRRYGE
                     DAVDRLRFIKRAQALGFSLDEVKLLLGLAIGEHCAETRALAEQKLALVEKKIADLRGM
                     QSALNKLARACGSGKRGRGCPIIESLSAGD"
BASE COUNT          905 a         1693 c         1681 g          877 t
ORIGIN      
        1 accagttcct gctgaagaag cacccgaagg gcacgacggg ctcgggcgcc gtggaccata
       61 tcgccttcgt cgccagcaat ccgcgcgagg tgcgcagccg catccggaaa aacagggtcg
      121 agatgcacta ccgcagcttc cccgaagcga agctgttcca gatcttcctc aaggaccccg
      181 acgacgtgac gatcgagctc aacttcctcg gcgaggcgat cgacgagaag caatggaagg
      241 gcaagggctc ggcctccgcg gtgaccctgg atggaggcaa agcgaagcgc cagaactaag
      301 atgctgtttg aaaagattca aatccgtgaa atagcgatca gaaatcgtat cgtcgtctcg
      361 ccgatgtgcc agtactcggc cgaggacggc catgtcaccg actggcactt cgtgcacctc
      421 ggcaagttcg cgcagggcgg cgccggcgtc gtgttcgtcg aggcgaccgc ggtggagaag
      481 cgcggccgca tcacgcacgg cgacacaggc atctgggacg acgcgcacat tgcgggtcta
      541 cgaaaaatct caggcttcat caggagccaa ggtgccgtgc cggcgatcca gctcgcccac
      601 gccggccgca agagcagcat ggcgcgcccc tggtacggca acggcccgct gacgcaggcc
      661 gacatcgagc gcggcgacaa gccctgggac atcgtcgcgc cgaccgaccg cccgatcggc
      721 gacggttgga tcaagcctcg tgcattggga agagacgatt taagcgatct tctcgacgcc
      781 tacagagccg ccgcccgccg tgcgctgaag gcgggcttcg acatcctgga aatccacgcc
      841 gcgcacggtt acctcctgca tacgttcctc tcaccgctct ccaacgacgg cagccgcgag
      901 gaccgcatgc gctttccgct cgaagtggcg cgggcggtca gggaggcgtg gccggcgcag
      961 aagccgctgt tctggcgctg ctcctcgatc gacgacgtgg aaggcggctg gagtatcgaa
     1021 gatacgattt gccttgcacg acaattaaga caaggcggtg tcgatgtggt cgattgctct
     1081 tcgggcggga tcctcggctc ggcgaccgcc gcgacgcgca cgctgctgcc gcgtgtgccc
     1141 ggctttcaga ttcctttcgc agaaaaagtc aaaaaagaag ccggcgtaat gaccatggcg
     1201 gtcggcttga tcctcacgcc gcagcaggcc gaggaagcgg tgcagtccgg gcgcgccgac
     1261 ctggtcgcga tcgcgcgcga ggccctctat gacccgaact ggccgctgca cgcagcgatc
     1321 gcgctcggcg tcgatccgga cttcgcgcag tggccggtgc agtacggctg gtggctgacg
     1381 cggcgcgagg gcctgctcag aaagctgggg gtacgcagat gaagacgatt cgcctttcag
     1441 cagcacagat gcagcagcgc atcgcgcgct tcaagtcgct cgcgccgctg ccgatccaga
     1501 tgggcgagat cccgctcgcg gcgcgcgacg tcgtctactc gcgcaagctc ctttcggtca
     1561 ttgggctgga tgaggaggca agcaccccca tcaacgccaa cgcgccgatc cgcggcgcgg
     1621 cgggcatgac catgacgcta gcggtatgcc cgccaggcca gggcccgggg ctgcactcgc
     1681 accgccagac ctacgagacc ttcacggtgc tcaagggccg cttcgaagtg agctggaacg
     1741 acgacggcgg cgaaagcgcg gtcctcgagc tcttcgacac ggtgtcgttc ccgccgggcg
     1801 tatgccgcgc cttccgcaac gtcggcaagg aggaaggcat cctgcaggtg atcatcaccg
     1861 gcggcgtgca cgacatgacc gatatcgatt tttccgagaa ggcgaaacgc gagatcgaag
     1921 ccatcggccc gggccttgca aaggagttcg agaaagcagg cttcagcttc accgccggag
     1981 ccgcatgaag cgtcgcagct tcctcggcgt tctgtgcgcg gcccgatcga atgaccgaac
     2041 tggggtctgg aaacgcgcaa tgagaaaaat ggggtctgtc cccatttatt ttttgaaccg
     2101 gttgcagtag gcgacgggcc cggtccaact ctgctcgacc accttgccgc cgctgaagac
     2161 gagcgtgcgg tcgcagcgca ccgggtcgcc ggccgggccg aagacgattc cgccagcgcc
     2221 gcccataccg ccgctgccgc ggatcactcc gtactcttcg gaaacccagg catgcgtttc
     2281 gcgcccgtcc tgcttgctgc tgcgcgcggg cgcgccccag gcgcgcacca cgtcgtcgta
     2341 gcgcgcgccc tgccaggagt ctcgcgccgc ggcgagatcc gggtcggccg tcgcgcagcc
     2401 ggaggcgagc gccgcagccg caacaacctt cgtggcgtta cgccacggca tgctcgagct
     2461 cgccgatgcg ctcgatccag agcttgagct tctcgccggg cttgaggaag cggccgcgcc
     2521 ccatgccgac gccggccggc gtgcccgtga ggatgagatc gccgggctgg agcgtgacgc
     2581 gcgaggagag catcgcgatc tgctcggcgg tgtcgaagat catcttgccg gtattcgagt
     2641 cctgcatcag ctcgccgtcg atccacagct tgagcccgag gctgtgcggg tcggggatct
     2701 ccgccgccgg cacgatccag ggcccgagcg ggcaggcgcc gtcgaagcat ttctggctca
     2761 cccagtcgta atgaaacggc gaggcgggcg ggttcttggc gcgcttcatc gcgtcgcgcg
     2821 ccgagaggtc gttggcgatg gtgtagccgg cgatgcattc gagcgcgcgc tccaccggca
     2881 catccttcgc cttgcggccg atcaccgccg cgagctcgat ctcccagtcg accgcgctcg
     2941 aatacgcagg cagcttcacc gccgcgcccg ggccgacgat cgagctcctc gagctcttga
     3001 cgaaatgcca cggcttctcg cccagctgct tcatgttcgg ccccggcgtg cggccctgcg
     3061 cgcgctccat ctcggccaca tggtcggtgt agtttgctcc ggcgcagtag atgttgcccg
     3121 ggtacaggat cggcgcgagc agcttcgccc gcttcagggg caggcccttt ttccccgccg
     3181 atttcttcgc cagcgccgag agctcgcgcc gcgcttttgc ccagtcctgc agcgccgcga
     3241 ggacgctggg gtaacttctg gtgaggtcgt agatctgctc cccgacgagg atgccgcctt
     3301 tcggctcgcg gccggcgaga tagctaagta tccgatagct catgcgctgt ctcctggact
     3361 attattccac ggaggaggaa aacccttgcg cgcgattctt gccggacttg cgttcctgct
     3421 tcttggaagt tgcgcgacgc cgcccgtcgc gccaaagacc gtcaacctga tcgtctttcc
     3481 tggcggcttc aactggccga tctgggtggc gcaggagaaa ggcctgttcg caaagaacgg
     3541 cctcgacgtc aaggtgacgc cgaccccgag ctcggtgttc cagctcacca atctcattgg
     3601 cggcaagttc gacattgcga tgaccgcgat agacaacctg atcgcctacc gcgagggcca
     3661 gggcgaggag ccggttcccg ggcctgatct gttcgcattc atgggtggcg acaacggctt
     3721 tttgcgccta gttgccgtac cggacgtgaa aactcatgga gacttgaagg ggaaaaccgt
     3781 ctctgtcgat gcgcgaacca ctggctacgc cttcgtgctc ttcgagatgc tcgagcgcgg
     3841 cgggctgcgg ctcgaccagg attacaaggt cgagcgcgcc ggcggtgtgc tgcagcgctt
     3901 ccaagcgctt atggaaaaaa agcacgcagc gaccctcctg ctgtcgcctt tcgagctgca
     3961 ggccgaggcg aagggcttca atcggctcgg caatgcgacc gatgtgctcg gcgcctacca
     4021 gggattggtg ggcggagcac gcaagagctg ggcggacgcg aaccgcgagg cggtagtggg
     4081 ctacattcgc gccttttccg aggcagtgga ctggctctac gacccacgca acaaggacga
     4141 ggcgatcgcg gtcttccgca agaacctgcc taacgtgccg gcgcaaggcg cgcaggcggc
     4201 gtacggcgta ctgctttcgc cgcgagaggg cttccagaag aaggcgcgca tcgacatgga
     4261 gggcgtgcgg acggtgctgc ggctccggtc gaaatacgcc gagccgcgga agttgctcac
     4321 cgatccggcg aagtactacg acgacagctt ttaccgcgcc gcgcttcgct aggggcgcca
     4381 aacggacaac gcgacgaggc aaccccatgc gccccgaccc tgccctgatc gaccgcgtcc
     4441 ttccctccga aaggctgatg aagcaggtga tcgggggcct ttcggtgttc actcttctaa
     4501 tgacgatccc gcaaatcgtg atgatctggg cgagccatca ggcagcgggc gtgtcgctgc
     4561 tgtcctgggg cgcctactgg atctcggctt tcgtctggtt cgggtacggc ctgcggaagc
     4621 acgacccgaa catctacctg ccttgcatcg gctggctgct gctcgacggc gccgtgatcg
     4681 ccgggaccgt gatttacggc tagtcgcctg cggaaaggct ctcgatgatc gggcagcccc
     4741 gcccgcgttt gccgctgccg caggcgcggg cgagcttgtt cagcgcgctc tgcatgccac
     4801 gcagatcggc aatcttcttc tccaccaacg ccagcttctg ctcggcaagc gcccgcgttt
     4861 ctgcgcagtg ctcgccgatc gcaagcccga gcagcagctt gacttcgtcg agggaaaacc
     4921 cgagggcttg ggcgcgcttg atgaacctca gccggtcgac tgcgtcctcg ccgtaacggc
     4981 gcaccgagcc gcgcgcccgc gcgggcttcg gcatcaaacc gaggcgctgg tagtagcgga
     5041 tcgtctcgac gctcaccgag gcgctccgcg cggtggcgcc aatggtcatc ccggtcatcg
     5101 agtgcacctc cggtactgcg agaacggcgt cggcccgcaa cgacccacgc acgagc
//
LOCUS       DASPXW010000179         2571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_53314, whole genome shotgun sequence.
ACCESSION   DASPXW010000179 DASPXW010000000
VERSION     DASPXW010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53314"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2189)
                     /locus_tag="VEL04_04245"
     CDS             complement(<1..2189)
                     /locus_tag="VEL04_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HYT47606.1"
                     /translation="MSRWQFWIDRGGTFTDIVARRPDGSIATRKLLSENPGRYADAAL
                     AGMRDFLGLRSDQAIPAEQIEAVKMGTTVATNALLERKGERTVLAITRGFRDALRIAY
                     QNRPRLFDRHIVLPEMLYSKVFEVAERIGARGNVVVPLDLLQTEKDLQKAFDEGFRSI
                     AIVFMHGYRFPEHESKVEALAEKIGFTQISVSHRVSPLMKLVGRGDTTVVDAYLSPIL
                     RRYVEGVAAELGGARLLFMQSNGGLTDARCFQGKDAILSGPAGGIVGAVRSSAAAGFE
                     RIIGFDMGGTSTDVSHYAGEFEREFETRVAGVRMRAPMMSIHTVAAGGGSILHFDGAR
                     YRVGPDSAGANPGPACYRRAGPLTVTDANVMLGKIQPQYFPKVFGVNGDEPLDGSAVS
                     QKFDQLKERINDNRSAEEVAEGFIQIAVGNMANAIKHISVQRGHDVTAYTLCCFGGAA
                     GQHACLVADALAMTRVFIHPHAGVLSAYGMGLADQVAMREQSLELELQDAGARALEDA
                     AEKLAIEAQKDLVSQGVDRLRIKLLRRAHLKYEGTDTALAVPFGPRAAMAQQFEAAYR
                     KQFSFLMPGKPLVVEAVSVEAIAAGEVIQEKKLSSGGMNGKPAQTVRMFTGGRWHEAP
                     LYRRDELAAGQSIDGPAIIAEANSTTVVEAEWRASVTQLDHLILERNKPRKTGVAVGT
                     AVDPVMLEIFNNLFMSIAEQMGLRLQNTAHSVNIKERLDFSCALFDAA"
     gene            complement(2186..>2571)
                     /locus_tag="VEL04_04250"
     CDS             complement(2186..>2571)
                     /locus_tag="VEL04_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47607.1"
                     /translation="RLGAPRCAGGMCVTLRDLARVGQWIIRNPTPWIEDIETSGDPQA
                     WAAGSFVEYFPGLPMRYRSKWYVLDGEAPLLFGMGIHGQNLFVDRRNDIVIAKLSSQA
                     LPLDAARIGLTLRTVTQIRNLLADA"
BASE COUNT          424 a          848 c          833 g          466 t
ORIGIN      
        1 gcggcatcga acagcgcgca cgaaaaatcc agccgctcct tgatattcac cgagtgggcg
       61 gtgttctgca ggcgcaggcc catctgctcg gcgatcgaca tgaagaggtt gttgaagatc
      121 tcgagcatta ccgggtcgac cgcggtgccg acggcgacgc cggtcttgcg gggcttgttt
      181 ctctcgagaa tcagatgatc cagctgcgtg acgctcgcgc gccactcggc ttcgacgacg
      241 gtggttgaat tcgcctcggc gatgatcgcc gggccgtcga tgctctgccc ggcggcgagc
      301 tcatcgcgcc ggtagagcgg cgcctcgtgc cagcgcccgc cggtgaacat gcgcacggtt
      361 tgagcgggtt ttccattcat tcctccagat gaaagctttt tttcttgaat cacctcccct
      421 gcggcgattg cctcgaccga taccgcttcc accaccagcg gttttccagg catgaggaag
      481 ctgaactgct tgcggtacgc ggcctcgaac tgctgcgcca tggcggcgcg cgggccgaac
      541 ggcacggcga gcgcggtgtc ggtgccttcg tatttcagat gcgcacgacg cagcagcttg
      601 atgcgcagtc gatctacccc ttgtgatacc aaatccttct gcgcctcgat ggcgagcttt
      661 tccgccgcgt cctcgagcgc gcgcgcgcca gcatcctgca gctcgagctc cagcgactgc
      721 tcgcgcatcg ccacctgatc cgcgagtccc atgccatacg ccgagagcac gccggcatgg
      781 ggatggatga acacgcgcgt catcgcgagc gcgtcggcga ccaggcaggc gtgctggccc
      841 gccgcgccgc cgaagcagca cagggtgtag gcggtgacgt cgtgcccgcg ctgcactgag
      901 atgtgcttga tcgcgttcgc catgttcccg accgcgatct ggatgaagcc ttcggcgacc
      961 tcttcagcgg agcggttgtc gttgattctt tctttcagct gatcgaattt ttgtgaaact
     1021 gcagaaccgt ccagcggctc gtccccgttc accccgaaaa ctttggggaa atactgcggc
     1081 tggatcttgc cgagcatgac gttggcgtcg gtcacggtga gcggcccggc gcggcggtag
     1141 caggctggtc ccggattcgc cccagccgaa tccggtccca cacgatagcg cgcaccgtcg
     1201 aaatgcagga tcgagccgcc gcctgccgcg accgtgtgga tgctcatcat cggcgcgcgc
     1261 atgcggaccc cggctacgcg ggtctcgaac tcgcgctcga actccccggc gtagtgcgag
     1321 acatcggtcg atgtcccgcc catgtcgaaa ccgatgatgc gttcaaaacc cgccgccgcc
     1381 gagctgcgca ccgcgccgac gatgcctccc gcgggtccgg aaaggatcgc gtccttgccc
     1441 tggaagcagc gcgcgtcggt caggccgcca ttggactgca tgaaaagcag ccgcgcgccg
     1501 cccagctcgg cggccacccc ctcgacatag cgccgcagga tcggcgagag ataggcgtcg
     1561 accaccgtgg tgtcgccgcg cccgacgagc ttcatcagcg ggctgacgcg gtgcgacacc
     1621 gagatctggg tgaaaccgat cttctccgcc agcgcctcga ctttcgactc gtgttccgga
     1681 aagcgatagc cgtgcatgaa cacgatggcg atcgagcgga atccttcgtc gaacgccttt
     1741 tgcaggtctt tctcggtctg tagaagatcg agcggcacga ccacgttccc tcgcgcgcca
     1801 attcgttccg cgacctcgaa gaccttcgaa taaagcattt cgggcagcac gatgtgacgg
     1861 tcgaacaggc gcggccggtt ctggtaggcg atgcgcagcg cgtcgcggaa gccgcgcgtg
     1921 atggcgagta ccgtgcgctc gccctttctt tcgagcagcg cgttggtggc cacggtggtg
     1981 cccatcttca ccgcttcgat ctgctcggcg ggaatcgcct gatcgctgcg caagccgaga
     2041 aaatcccgca tcccggcgag cgcggcgtcc gcgtagcgcc cgggattctc ggagagcagc
     2101 ttgcgagtcg cgatcgagcc gtcgggccgg cgcgcgacga tgtcggtgaa ggtgccgccg
     2161 cggtcgatcc agaactgcca gcggctcatg cgtcggcgag caggttcctg atttgcgtca
     2221 ccgtgcgcag ggtgagcccg atgcgcgccg cgtcgagcgg cagcgcctgc gacgagagct
     2281 tcgcgatcac gatgtcgttg cgccggtcga cgaacaggtt ctgcccgtgg atgcccatgc
     2341 cgaaaagcag cggcgcctcg ccgtccagca cataccactt gctgcgatag cgcatcggca
     2401 ggccggggaa gtattcgacg aagctgccgg cagcccaggc ctgcgggtca ccgctggttt
     2461 caatgtcctc gatccagggg gtggggttgc ggatgatcca ttggccgacg cgcgccaggt
     2521 cccgcagggt gacgcacatc cctcccgcgc agcgcggcgc gccgaggcga t
//
LOCUS       DASPXW010000180         2853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1188005, whole genome shotgun sequence.
ACCESSION   DASPXW010000180 DASPXW010000000
VERSION     DASPXW010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188005"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="VEL04_04255"
     CDS             complement(<1..631)
                     /locus_tag="VEL04_04255"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYT47608.1"
                     /translation="MKSASIFVGALLAATVQAQEPPFPQKAPIEITVLFPAGSSADVT
                     ARLLADGMSRRLGGAKVLVINRPGAGGAIGYKYVASQKADGYSIVWNSNSISTTFHSG
                     MLPFDYRAFDAVARVLVESPVIAVRGDSKWKSLGELIAEAKSRPRQVTVGNSGIGSHT
                     HISSVALFKAAGTEVVDVPFGAAQVVPSLLGGQVDAVVQLPAALSGYVKT"
     gene            complement(647..1654)
                     /locus_tag="VEL04_04260"
     CDS             complement(647..1654)
                     /locus_tag="VEL04_04260"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYT47609.1"
                     /translation="MAFEPLRVACVGMGWWSDVLADAIQRSNKLKIVACYTRSEDKRR
                     AFAAKYGCLAAPSYEAVLGDRAIEAIVNTTPNDVHLETTRAAAAAGKHVFLDKPIANT
                     VSEGRAITEACRKAGVVLALGYQRRRESHFRWIRERIDAGAFGKLVNAEANISRDRLG
                     KIDLNSWRYTAAGMPGGVMLQIGIHYTDVLEYLLGPIKAVNGRFAQLVLPGDNPDVAS
                     MVLEHENGALSTLNASYASASEYYLMNVYGKEASAYYDMHSGLRHLKRGSKSASPIAC
                     ALNDPIVEELEEFARAVRGDGAPEMGGESATASLAVIRAGIVSAREGRRVEVAEMLSK
                     D"
     gene            complement(1660..2100)
                     /locus_tag="VEL04_04265"
     CDS             complement(1660..2100)
                     /locus_tag="VEL04_04265"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HYT47610.1"
                     /translation="MFVANAKRLTHAGARKIADTAVAKARQAGIAISVAVADAGGHLI
                     ALERMDGGRFHSLHSATTKAVCAASNKRPTSSHGAQGQALDVAHALGLALAAGPGRWT
                     AMEGGCPVIVEGECIGGVGVSGGDWATDERIAREAVEAIGATAK"
     gene            complement(2175..>2853)
                     /locus_tag="VEL04_04270"
     CDS             complement(2175..>2853)
                     /locus_tag="VEL04_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYT47611.1"
                     /translation="ARLIGAPPGYVGYEEGGHLTETVRRKPYSVILLDEIEKAHPDVF
                     NVLLQVLDDGRMTDGQGRTVDFKNTVIVMTSNLGSHMIQQMQGDDPGLIKTAVLAEVK
                     TQFRPEFVNRIDEIVVFHALDEKHIRNIARIQLRYLEERLAKLEMGLEVGDSALAQLA
                     KAGFDPVYGARPLKRAIQQSIENPLAKQILEGRFAAKDLIRVEYRGGAMQFEKSAARP
                     THKAAIG"
BASE COUNT          449 a          962 c          907 g          535 t
ORIGIN      
        1 ctgtcttcac gtacccggac agcgccgcgg gcagttgcac cactgcatcc acttgtccgc
       61 cgagcaggct cggcacgact tgcgcggcgc cgaacggcac gtccacgacc tcggtgcccg
      121 cggccttgaa cagcgcgacc gaggaaatat gggtgtggct gccgatcccc gaattgccga
      181 ccgtgacctg cctgggtcgc gatttcgctt cggcgatgag ctcacccagc gatttccact
      241 tcgagtcgcc gcgcaccgcg atcaccggcg actcgaccag cacgcgcgcc accgcgtcga
      301 aggcgcggta atcgaacggc agcattccgg aatggaaggt cgtcgagatc gaattggaat
      361 tccacacgat cgagtatccg tcggcctttt gcgaggctac atatttgtag ccgatcgcgc
      421 ccccggcgcc cgggcggttg atcacgagca ctttggcgcc gccgagccgc ctcgacatcc
      481 cgtcggcgag cagccgcgcc gtgacgtccg ccgaggagcc ggcgggaaag agcaccgtga
      541 tctcgatcgg ggccttctgc gggaacggag gttcctgcgc ctggacggtc gccgcgagga
      601 gggcaccgac gaatatcgaa gctgatttca tgaatatctc ccgacttcaa tccttcgaca
      661 gcatttctgc gacctcgacc cggcgcccct cgcgcgccga gacgattccc gcgcggatca
      721 ccgcaagcga agcagtcgcg ctctcgccgc ccatctcggg cgcgccgtcc ccgcgcaccg
      781 cgcgggcgaa ctcctcgagc tcctcgacga tggggtcatt cagagcgcac gcgatcgggc
      841 tcgcactctt gctcccgcgc ttcaaatgcc gcaggcccga atgcatatcg tagtacgcgc
      901 tcgcctcctt gccgtagaca ttcatcaggt agtactcgga ggccgaggcg tagctcgcgt
      961 tgagcgtcga cagggcgccg ttctcgtgct cgaggaccat gctcgcgacg tccgggttgt
     1021 cgcccgggag cacgagctgc gcgaaccttc cgttcacggc cttgatgggc ccgagaaggt
     1081 attcgagcac gtcagtgtag tgaatgccga tctgcagcat cacgcccccc ggcatccccg
     1141 cggcggtata acgccaggag ttgagatcga tcttgccgag ccggtcgcgg ctgatgttgg
     1201 cctccgcgtt gacgagcttt ccgaatgcgc ccgcgtcgat tctctcgcgg atccatcgaa
     1261 aatggctctc gcggcgccgc tggtatccca aggcgagcac gaccccggcc ttgcggcagg
     1321 cctcggtgat ggcgcgccct tcggagacgg tattggcgat cggcttgtcg agaaacacgt
     1381 gcttccccgc cgcagcggcg gcgcgcgtcg tttccagatg cacgtcgttc ggcgtggtgt
     1441 tgacgatcgc ctcgatcgca cgatccccca gaacagcttc gtaactcggg gcggccaggc
     1501 agccgtattt cgcggcgaag gcgcggcgct tgtcctcgga gcgcgtgtag caagcgacga
     1561 tcttcagttt attcgaccgc tggatggcgt cggcgaggac gtccgaccac cagcccatcc
     1621 cgacgcaggc gacgcgcagc ggctcgaacg ccatcgttgt tacttcgcgg tcgcgccaat
     1681 ggcttcgacg gcttcgcgtg cgatgcgctc gtcggtcgcc cagtcgccgc cgctcactcc
     1741 cacgccacct atgcactcgc cttccacgat cacggggcag ccgccctcca tcgccgtcca
     1801 gcgcccgggt cccgccgcga gcgcgagccc gagcgcgtgg gccacgtcga gcgcctggcc
     1861 ttgcgcgccg tgcgacgagg tcggccgctt gttcgacgct gcgcacacgg ctttggtggt
     1921 cgcagagtgc agggagtgga agcggcctcc atccatgcgc tcgagcgcga tgaggtgccc
     1981 gcccgcgtcg gccacggcga ccgagattgc aatcccggcc tgcctggctt tcgctacggc
     2041 agtatcggcg atcttcctgg cgcctgcgtg agtcaggcgc ttggcgttgg cgacgaacat
     2101 gagacttgaa cgagacaaga ggccgtctgg aaagctcgaa ttctgcccgg ggccccgcgt
     2161 cccatcaaga cttttcagcc aatcgccgct ttatgggtgg gtcttgcggc ggatttctcg
     2221 aactgcatcg cgccgccgcg atattcgacg cgaatgaggt ctttggcggc gaacctgcct
     2281 tcgagaatct gtttcgcgag cgggttttcg atgctctgct ggatcgcgcg cttgagcggc
     2341 cgcgcgccgt agaccggatc gaagccggcc ttggcgagct gtgcgagagc gctgtctccc
     2401 acttccagtc ccatctccag cttcgccagc ctctcctcga ggtaacgcag ctggatcctg
     2461 gcgatgtttc ggatgtgctt ttcgtcgagc gcgtggaaca ccacgatctc gtcgatgcgg
     2521 ttgacgaact cgggcctgaa ctgcgtcttc acttccgcca ggaccgcggt cttgatgagg
     2581 ccgggatcgt cgccctgcat ctgctggatc atgtgcgagc cgaggttgga ggtcatcacg
     2641 atgaccgtgt tcttgaaatc cacggtgcgg ccctggccgt cggtcatgcg gccgtcgtcc
     2701 agaacctgca ggagcacgtt gaacacgtcg gggtgagcct tttcgatctc gtcgagcagg
     2761 atcacgctgt agggcttgcg cctcaccgtc tcggtgagat gcccgccttc ctcgtaaccg
     2821 acgtagccgg gcggcgcacc gatcaggcgc gcg
//
LOCUS       DASPXW010000181         2391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_430241, whole genome shotgun sequence.
ACCESSION   DASPXW010000181 DASPXW010000000
VERSION     DASPXW010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430241"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VEL04_04275"
     CDS             complement(<1..478)
                     /locus_tag="VEL04_04275"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="FabH; beta-ketoacyl-acyl carrier protein synthase
                     III; catalyzes the condensation of acetyl-CoA with
                     malonyl-ACP to initiate cycles of fatty acid elongation;
                     differs from 3-oxoacyl-(acyl carrier protein) synthase I
                     and II in that it utilizes CoA thioesters as primers
                     rather than acyl-ACPs; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HYT47612.1"
                     /translation="MTAETPIQAAITGVHGHLPADVLDNETLARMVDTSDAWITERTG
                     IKERRILKGEGLGTSHMAAEAVRGLLERTATRPQDVDLLICATTTPDFVFPSTANIVC
                     DMVGIRNIGSFDVQAACSGFVYALTIAAQFIGTGRARKIVVVGADKMSAIIDYTDRA"
     gene            complement(584..1795)
                     /gene="lpxB"
                     /locus_tag="VEL04_04280"
     CDS             complement(584..1795)
                     /gene="lpxB"
                     /locus_tag="VEL04_04280"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HYT47613.1"
                     /translation="MNGQQPRKMLDKPLDVFMVAGESSADDLGARFMRSLSALRPVSY
                     RGVGGPRMNAAGLASLYPAQGLTSIGIATVIVRLPLLLRRMRETVDAIVAAPPDVLVL
                     IDAPDFTHRIAARVRRRLPRLPIVKYVSPSVWVWRSGRARAMRGSIDLVLALLPFEPE
                     VYRQLGGPDCVYVGHSLLEHLADLRPAADEERQRAASPPLVLALPGSRTQEVERLTKI
                     FGETLGMVAAKSGPLEVVLPTVPHLVGRLSELTSRWPIRPRILVDEAEKHAAFRRARA
                     ALAASGTVTLELALAGVPSVAAYRIAALEGLMFRAVRRMHPVIKVRSIILANLVLGEW
                     AIPEFVQRQCTAENLAAALIDILGDTPARARQIEAFKRLEAIMGASGVGPSKRAAQAV
                     LDLVERRSAMS"
     gene            complement(1798..>2391)
                     /gene="lpxI"
                     /locus_tag="VEL04_04285"
     CDS             complement(1798..>2391)
                     /gene="lpxI"
                     /locus_tag="VEL04_04285"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF017989.4"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HYT47614.1"
                     /translation="PRLIKAFAGGDDHLLTRICRIVEDRGFRLLGAHEVAPEILVPAG
                     QLGRHAPSERDRADIAVGRALLAATGAFDIGQAVVVAGNRVIAIEAAEGTDRMLARIA
                     QLRREGGLKLPDKSGVLVKAPKPSQDRRFDLPSVGARTIEVAAAASLAGIAVEARGAV
                     TADLSEMVRQADAAGLFVVGVPAGEPMRGDTRGDMRG"
BASE COUNT          415 a          808 c          795 g          373 t
ORIGIN      
        1 tggcgcggtc ggtgtagtcg atgatcgccg acatcttgtc ggcgccgaca accacgattt
       61 tgcgcgcccg accggtgccg atgaactgcg ccgcgatggt gagcgcgtag acgaagcccg
      121 aacaggccgc ctgcacatcg aagctgccga tgttgcggat acccaccatg tcgcagacga
      181 tgttggcggt ggagggaaac acaaaatccg gcgtggtggt ggcgcagatc aggagatcca
      241 catcttgcgg tcgcgtagcg gtccgctcga gcaggccgcg caccgcttcg gctgccatgt
      301 gcgaggtgcc gaggccttcg cccttcagga tccgtcgctc cttgatgccg gtgcgttcgg
      361 tgatccacgc atcgctggta tcgaccatgc gcgcgagcgt ctcgttgtcg agcacgtcgg
      421 cggggaggtg gccgtgtacg cccgtgatgg cagcctggat cggtgtctca gcggtcacat
      481 cgtgccttca tcaatttttg cgcaagcgct tggtatagag gcaagccgct tgaggaccaa
      541 gcttgcaggc caagcgatgc accagggatt gccgggcttt tcatcaactc attgctgatc
      601 gccgttcgac aagatcaagc acggcctgcg cggcgcgctt gctcggaccg acaccgctgg
      661 cacccatgat cgcttcgagc cgtttgaacg cctcgatctg gcgcgcacga gcgggcgtgt
      721 cgcccaggat gtcgatgagc gccgctgcca gattctccgc cgtgcactgc ctttgcacga
      781 actcgggaat cgcccattca cccagcacca ggttcgccag gatgatcgag cggactttga
      841 tcacgggatg catcctcctc accgcccgaa acatgagccc ctccagcgcg gcgatgcgat
      901 aggccgcgac gctcggcacg ccggcaagcg caagctcgag cgtcaccgtg ccggaggcgg
      961 cgagcgccgc gcgtgcgcgg cggaaggctg cgtgcttctc ggcctcatcg accaggatgc
     1021 gcggccggat cggccagcgc gatgtcagct ccgacagccg tcctacgaga tgcggcactg
     1081 tcggcagcac cacctcgagc ggtcctgatt tggcagcgac catgccgagc gtctcgccaa
     1141 atatcttggt aagacgctcg acctcttgtg tgcggctgcc cggcagcgcg agcaccagcg
     1201 gcggcgaagc ggcgcgctgc cgctcctcgt ccgcggcggg gcgcagatca gcgagatgct
     1261 caagcaagga gtgcccgaca tagacgcagt ccggaccgcc cagctgacga tacacctcgg
     1321 gctcgaacgg cagcagcgcg agcacgagat caatcgagcc ccgcatcgcg cgggcgcgcc
     1381 cggaccgcca gacccacaca ctgggcgaca cgtatttgac gatcggcaga cgcggcagcc
     1441 gccgccgcac acgagccgcg atgcgatgcg tgaaatcagg agcatcgatg agcaccagca
     1501 cgtcgggcgg cgcggccacg atggcgtcga cagtctcgcg catgcgccgc agcagcagcg
     1561 gcagcctgac gatcacggtt gcgatgccga tcgaggtgag accctgtgcc ggatacaggc
     1621 ttgcgaggcc tgctgcgttc atgcgtgggc cgccgacgcc gcgatacgag accggacgga
     1681 gcgcagaaag cgatcgcatg aagcgtgcgc cgaggtcgtc ggcggaactc tcacccgcca
     1741 ccatgaacac gtcgagcggc ttgtcgagca tcttgcgcgg ttgttgcccg ttcacggcta
     1801 tcctcgcatg tcccctcgcg tgtcccctcg catgggctca cctgcgggaa ccccgaccac
     1861 aaagagcccc gccgcatcgg cctggcggac catctcgctg agatcagcgg tgacggcgcc
     1921 gcgggcctcg accgcgatgc cggcgaggct cgcggcggca gcgacctcga tagtgcgggc
     1981 gcccaccgac ggcagatcaa agcggcggtc ctgggaaggc ttcggcgcct tgaccaggac
     2041 gccgctcttg tccggaagct tgagaccgcc ctcgcggcgc aattgcgcga tgcgggccag
     2101 catgcggtcg gtaccctccg ccgcctcgat tgcgatcaca cgattgccgg ctaccaccac
     2161 cgcttggccg atgtcgaacg ctccggtggc cgcaagcagc gcccggccaa ccgcgatgtc
     2221 ggcgcgatcg cgctcgctgg gggcatgtcg cccgagctgg ccggcgggca ccagaatctc
     2281 gggcgcgacc tcgtgagcgc ccaggagccg aaaaccccgg tcctcgacga tgcggcagat
     2341 gcgcgtgagc agatggtcat caccgcccgc aaacgccttg atgaggcggg g
//
LOCUS       DASPXW010000182         3820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_933258, whole genome shotgun sequence.
ACCESSION   DASPXW010000182 DASPXW010000000
VERSION     DASPXW010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933258"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="VEL04_04290"
     CDS             <1..718
                     /locus_tag="VEL04_04290"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HYT47615.1"
                     /translation="FSSMKLLSRIANTGKSGARAEDRVFNAILDAVLDHRLAPGTKLT
                     ERELTEILGASRGAVRAALSRLGHSLLVELRPNRGAVIANPSLAETRDLFEARRVLEA
                     SLVQRLARSLTAKQIAELRAFVADEQRAYDRGDRKGGQRRSIRFHKLLSELGGNAVLD
                     RFMEQLICRTPLLALARTGRIAYCGAHEHREVVEALARRDAPAAMRRMTEHLANVERQ
                     LLAGEQPQLPSSLAAALGTA"
     gene            715..1578
                     /locus_tag="VEL04_04295"
     CDS             715..1578
                     /locus_tag="VEL04_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012354047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HYT47616.1"
                     /translation="MRRFAYFAPATLDEAVALLGRYDGKASVLAGGTDLLVEIKEQLR
                     RPDQIIDIKKIPAMAQLAYHPDHGLKIGALVTTRQIETSPVVLEKYPGLAQAVRELGS
                     IQIRNRATLAGNVCRASPSADTLPPLMADRARVTLYGPRGSRTVALEAFFTGPGRTVM
                     RPDELMTEIRLPPPPPRTGKAYIKHGRRKAMELATVGVAVSVTLQENRFREVDIVLGA
                     VAPTPLRALKAEAILRTQEVSEELIEEAAIAAMSESKPISNVRASADYRREMVAVLTR
                     RALRQALGLIR"
     gene            1575..2039
                     /locus_tag="VEL04_04300"
     CDS             1575..2039
                     /locus_tag="VEL04_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010811673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HYT47617.1"
                     /translation="MKTNLSLRVNGEPRELAVEPYRSLLDVLRNDAGLTGTKKGCDVG
                     DCGACTVLLDGKPVCACLVLGVEAQGCEVVTIEGIGNSPGGLHPLQENFMKCGGAQCG
                     FCTPGVIMMAKALLDENPRPTEEEIRFAIAGNICRCTGYTKIIEAIFATANS"
     gene            2036..>3820
                     /locus_tag="VEL04_04305"
     CDS             2036..>3820
                     /locus_tag="VEL04_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HYT47618.1"
                     /translation="MSDLSVVGTSVTRPDMLGKVTGSASYAGDVKLAGMLHGKIKRST
                     VAHARIRRIDASNALSLPGVTAVLSRENVPRVLHYGSPHPRSASVTRDQYIFDTKVRY
                     WGEGIAAVAAASEEIAEEALELIEVEYQELPAALTVEQALAPGAPLIHDTEHNLVTEP
                     IVVRRGDVAEGFAAADLIVEGRYEMGRPTPAYMEPNVCVAQWDQEGRLTMWVSTQTPF
                     MVRGILAEVLGLPLNKVRVLVDHMGGGFGAKQDLFQHEFLCALLARETRRPVRMEYTR
                     KETFLGGRSRHPCSIWLKQGFRKDGSLTAREARLTYNSGAYGSHGPGVTAVGTTALTS
                     LYRCDNVHLEGRCVYTNTPIAGAYRGYGVTQSYYALDIQMDEAAARLGIDPVEIRMRN
                     AVREGDLSPSGHPVVGHGLEDCIRRGMQEANWPRKKTSEKGPSRRGWGVGCEMHGSSA
                     YPGIKEQGNATVKMSEDGTVTLYTGTTGLGTGAHTALAQIVAEELGLAFGDVSVVHGD
                     TDVVPWDIGAFASHTTYMGGRAAQMAAAALKKQLLERAAEDLEVAAADLEVRRGTIFV
                     KGSDRSVSVRDAVRPKKGVPAAHFVASST"
BASE COUNT          647 a         1279 c         1325 g          569 t
ORIGIN      
        1 cttctcgagc atgaagctgc tatcccgcat cgcgaatact ggtaagagcg gcgcgcgcgc
       61 cgaggatcgg gtgttcaacg ccatcctcga cgccgtgctc gaccatcgcc tcgcgccggg
      121 caccaagctg acggagcgcg agctcacaga aatcctcggc gccagccgcg gcgcggtgcg
      181 cgcggcgctc tcgcgcctcg gccacagcct gctggtcgag ctgcggccga accgcggcgc
      241 ggtgatcgcc aatccttcgc tcgccgagac gcgcgacctg ttcgaggcgc gccgcgtgct
      301 cgaggcctcg ctggtgcagc ggctcgcgcg ctcgctcacg gcgaagcaga tcgccgagct
      361 gcgcgccttc gtcgccgacg agcagcgcgc ctacgaccgc ggcgaccgca agggcgggca
      421 gcgccgctcg atccggttcc acaagctgct gtccgagctt ggcggcaacg cggtgctcga
      481 ccgcttcatg gagcagctca tctgccgcac gccgctgctt gcgctcgcgc gcaccgggcg
      541 catcgcctac tgcggcgcgc acgagcaccg cgaggtggtc gaggcgctcg cgcgccgcga
      601 tgcgccggcc gcaatgcggc gcatgaccga gcacctggcg aacgtcgagc gccagctgct
      661 cgccggcgag cagccacagc tgccctcgag cctggccgcg gcgctcggca ccgcgtgaga
      721 cgcttcgcct acttcgcgcc tgccacgctc gacgaggcgg tggcgctgct cggccgctat
      781 gacggcaaag cgagcgtgct cgccggtggc accgacctgc tggtggaaat caaggagcag
      841 cttcgccgcc ccgaccagat catcgacatc aagaagatac cggcaatggc gcagttggct
      901 tatcaccctg atcatggatt gaagatcggg gcgctggtca cgacgaggca gatcgagacc
      961 tcgccggtgg tgctcgagaa gtaccccggg ctcgcgcagg cggtgcgcga gctgggctct
     1021 atccagatcc gcaaccgcgc cacgctcgcc ggaaatgtct gccgcgcttc gccctcggca
     1081 gacacgctgc cgccgctgat ggccgatcgc gcgcgcgtga cgctctacgg cccgcgcggc
     1141 agccgcaccg tcgcgctcga ggcgtttttt acgggtccgg gcaggaccgt gatgcggccc
     1201 gacgagctga tgaccgagat caggcttccg ccgccgccgc cgcgcaccgg caaggcttac
     1261 atcaagcacg gccggcgcaa ggcgatggag ctcgccacgg taggcgtggc cgtgtcggtg
     1321 actctccagg agaacagatt cagagaagtc gacatcgtgc tgggcgcggt cgcgccgacg
     1381 ccgctgcgcg cgctcaaggc cgaggcgatc ctgcgtactc aggaagtcag tgaggagttg
     1441 atagaagaag cggcaattgc tgcgatgagc gaatcgaagc ccatcagcaa cgtgcgcgcg
     1501 agcgccgatt accggcgcga gatggtcgca gtgctgacgc ggcgcgcgct gcggcaagca
     1561 ttgggcttga tcaggtgaaa acaaacctgt cgttgcgcgt aaatggcgag ccgcgcgagc
     1621 tcgcggtcga gccttaccgc agcctgctcg acgtgctgcg caacgacgcc ggcctcaccg
     1681 gcaccaagaa aggctgcgat gtcggcgact gcggcgcctg caccgtgctg ctcgacggga
     1741 aaccggtgtg cgcgtgcctg gtgctcggcg tcgaggcgca gggctgcgag gtcgtgacca
     1801 tcgaaggcat cgggaattcc cccggcgggc tgcatccgct gcaagagaat ttcatgaagt
     1861 gcggcggcgc gcagtgcggc ttctgcacgc cgggcgtgat catgatggcg aaagcgctgc
     1921 tcgatgaaaa tccgcgcccc acagaggagg agatccgctt tgccattgca gggaatatct
     1981 gccgctgcac cggctacacc aagatcatcg aagcgatttt cgccaccgct aattcatgag
     2041 cgatctatct gtcgtcggca cgagcgtcac gcgccccgac atgcttggca aggtgacggg
     2101 cagcgcgagc tatgccggcg acgtcaagct cgccggcatg ctgcacggca agatcaagcg
     2161 cagcaccgtg gcgcacgccc gcatccggcg cattgatgcc tcgaacgcgc tctcgctccc
     2221 cggggtaacg gctgtgttaa gtcgtgaaaa cgtcccccgc gtactgcact atgggtcgcc
     2281 gcacccgcgc tcggcgtcgg tgacgcgcga ccagtacatt ttcgacacca aggtgcgcta
     2341 ctggggcgaa ggcatcgcgg ccgtagccgc ggcgagcgag gaaatcgccg aggaggcgct
     2401 cgagctgatc gaggtcgagt accaggagct cccggccgcg ctcaccgtcg agcaggcgct
     2461 cgcccccggt gcgccgctga tccacgacac ggagcacaac cttgtgacgg agccgatcgt
     2521 ggtgaggcgc ggtgacgtgg ccgagggctt tgccgcagcc gacctcatcg tcgaaggccg
     2581 ctacgagatg gggcggccga cgccggccta catggagccg aacgtctgcg tggcgcagtg
     2641 ggaccaggag gggcgcctca cgatgtgggt ctcgacgcag acgccgttca tggtgcgcgg
     2701 catcctcgcg gaggtgctcg gcctgccgct caacaaggtg cgcgtgctgg tcgaccacat
     2761 gggcggcggc ttcggcgcca agcaggacct tttccagcac gagttcctgt gtgcgctgct
     2821 tgcgcgcgag acgcggcggc cggtacgcat ggagtacacg cgcaaggaga cctttctcgg
     2881 cggccgctcg cgccacccgt gcagcatctg gctcaagcag ggcttccgca aggacggcag
     2941 cctcaccgcg cgcgaggcgc ggctcaccta caacagcggc gcctacggct cgcacggccc
     3001 tggcgtcacc gcggtgggca ctaccgcgct gacatcgctt tatcgctgcg acaacgtgca
     3061 cctcgagggc cgctgcgtgt acaccaatac gccgatcgcc ggcgcgtatc gcggctacgg
     3121 cgtgacgcag agctactacg cgctcgacat ccagatggac gaggccgccg cgcggctcgg
     3181 catcgacccg gtcgagatca ggatgcggaa cgcggtgcgc gagggcgatc tctcgccttc
     3241 cggccacccg gtcgtcggcc acgggctgga ggactgcatt cgccgcggca tgcaggaggc
     3301 gaactggccg agaaagaaaa cctccgagaa agggccttcg cgccgcggct ggggcgtcgg
     3361 ctgcgagatg cacggctcga gcgcgtatcc gggcatcaag gagcagggca acgccacggt
     3421 gaagatgagc gaggacggca cggtgacgct ctataccggc accaccggcc tcggcaccgg
     3481 cgcgcacacc gcgctcgcac agatcgtcgc cgaggagctc ggccttgcgt tcggcgatgt
     3541 ttcggtggtg catggcgata ccgacgtcgt gccctgggac atcggtgcct tcgcgagcca
     3601 taccacctac atgggcgggc gcgccgcgca gatggcggcg gcggcgctga agaagcagtt
     3661 gctcgagcgc gctgccgaag atctggaagt tgctgctgcc gatctggagg tccgccgagg
     3721 aacgattttc gtaaaaggct ctgatcgcag cgtgagcgtg cgcgacgcgg tgcgcccgaa
     3781 gaagggcgtt ccggcggcgc atttcgtggc gagcagcact
//
LOCUS       DASPXW010000183         5808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1816815, whole genome shotgun sequence.
ACCESSION   DASPXW010000183 DASPXW010000000
VERSION     DASPXW010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5808
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1816815"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..502)
                     /locus_tag="VEL04_04310"
     CDS             complement(86..502)
                     /locus_tag="VEL04_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HYT47619.1"
                     /translation="MECTVRWTSGMTFLAETASNHVVAMDGAPEGGGRNLAPRPMELV
                     LAGTGGCTAYDVVLILTKSGQNVTGCEVRLTSERAEADPKVFTAIHMHFVVRGRALKR
                     NLVEQAIRLSHEKYCSASIMLGKTAAITKDFEIIED"
     gene            743..1171
                     /gene="rplM"
                     /locus_tag="VEL04_04315"
     CDS             743..1171
                     /gene="rplM"
                     /locus_tag="VEL04_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005007737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HYT47620.1"
                     /translation="MTTYTAKVGEIENRWVVVDAQGKVLGRLAAQIAARLRGKHKPEY
                     TPHVDTGDYVVVVNAAKLRVTGKKAERKIYYRHSGFPGGIKQTNFAKLHAAKPERVLQ
                     RAVKGMLPKGPLGYAMIRKLKIYSGGSHPHSAQQPTTLEI"
     gene            1181..1573
                     /gene="rpsI"
                     /locus_tag="VEL04_04320"
     CDS             1181..1573
                     /gene="rpsI"
                     /locus_tag="VEL04_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HYT47621.1"
                     /translation="MVGDYYYGTGRRKSAVARVFLKPGKGGFVVNGKPVDEFFGRETG
                     RMVVRQPLELTKSQSSFDIMVNVYGGGESGQAGAVRHGITRALIEYDAALKGVLSTAG
                     LVTRDAREVERKKVGLHKARRRKQYSKR"
     gene            1647..2687
                     /gene="argC"
                     /locus_tag="VEL04_04325"
     CDS             1647..2687
                     /gene="argC"
                     /locus_tag="VEL04_04325"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HYT47622.1"
                     /translation="MIKAGIVGGTGYTGVELLRLLVQHPQVELKVITSRKEAGTPVAA
                     MFSSLRGRVALAFTEPDAKTLRGCDVVFFATPHGVAMKEARTLLDGGAKVVDLSADFR
                     LQDLAEWERWYKMKHAAPELLREAVYGLCELNRERIRRARLVANPGCYPTAIQLGFLP
                     LVEAGAVDLDHLIADAKSGVSGAGRKAELSLTFSEASDNFMAYNVPGHRHRPEIRESL
                     AQAAKREVGLVFTSHLTPIIRGIHATLYGRVTKELDFQQLFEKRYASEPFVDVMPPGS
                     HPDTRSVRAANVCRIAVHRPPEGARQADTLVVLSVIDNLVKGASGQAVQNMNLMFGLP
                     EAAGLGQLPVVP"
     gene            2687..3448
                     /locus_tag="VEL04_04330"
     CDS             2687..3448
                     /locus_tag="VEL04_04330"
                     /inference="COORDINATES: protein motif:HMM:NF042896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6776 family protein"
                     /protein_id="HYT47623.1"
                     /translation="MRAFADRIGKLRQRFGIAAPRVAVHSHIAWYWRWVGVAVLVGVS
                     AAAAAWIYDAGRRFAGFDRSEVQQELAAALADLATARDELERLRAIANAADSRVSIER
                     TAQQKLAQQIRALEQENARVREELATIETMLSSEARSAAALSIYRFKVEPDVLPGEYR
                     YHLLLLTPTTRRERDFNGRLELVVSLTEGGQSVMMSFPEPRDAGASAFRLAFKYFRRV
                     EGSFRVNPKARVESVQVRVYEAGSNQPRATHTVTL"
     gene            3473..3871
                     /locus_tag="VEL04_04335"
     CDS             3473..3871
                     /locus_tag="VEL04_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HYT47624.1"
                     /translation="MFNKTSKPQNRIDSLIGATTRIEGNVIFSGGLRVDGMVRGNVAA
                     LSDQPGTLVLSADARIDGEVQAAHIVVNGTINGPVHATETLELQAGSQVKGDVYYKSI
                     EIHQGAVVEGRLVHHPADMKGVELKLASGG"
     gene            3901..4263
                     /gene="erpA"
                     /locus_tag="VEL04_04340"
     CDS             3901..4263
                     /gene="erpA"
                     /locus_tag="VEL04_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HYT47625.1"
                     /translation="MNAVTESVTEMPAPLVFTDNAATKVKQLIDEEGNPELKLRVFVT
                     GGGCSGFQYGFTFDEMQNEDDAVMEKNGVKLLIDPMSYQYLVGSEIDYQEGLEGAQFV
                     IKNPGAKSTCGCGSSFST"
     gene            4391..5284
                     /locus_tag="VEL04_04345"
     CDS             4391..5284
                     /locus_tag="VEL04_04345"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HYT47626.1"
                     /translation="MQEGVEWTAAAAKEGSGEAAYLLAVLASSGIGVPQDLKASLHYL
                     QQAAERGHRPAQTELAALVGNWRLVREISSGKIPRQDTWDRLRAAVDTEAWLSVPHGS
                     VFSAEPRIAAVKGFISAHICDWLVRLGKPHLQQAEIYDTESGDLREDGRRSNSATGLG
                     LARMDMVLAFVRARIAALAELPVFALETSQVLHYEVGQQFSPHHDFLDVSFPGLARKV
                     ASEGQRALTLLIYLNEDYEGGDTAFPTLGRSFKGRKGDALIFWNVTEDGAPDWRTLHV
                     GTAPTSGEKWLFSQWIRIRAA"
     gene            complement(5310..>5808)
                     /locus_tag="VEL04_04350"
     CDS             complement(5310..>5808)
                     /locus_tag="VEL04_04350"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HYT47627.1"
                     /translation="LRSPLDFARVRSGFGLRVHPIFRTWRAHKGIDYVAAAGTRVRAV
                     GDGIVEFVGEKGGYGKAVVLRHHGQYATLYAHLSRFPAGLHRGARMAQNDTIGFVGRT
                     GWATGPHLHYEFQVAGQARNPLSIAMPAAQPVPAQQLPEFRIHAAPLIARLDLLADTN
                     LALLD"
BASE COUNT         1076 a         1786 c         1945 g         1001 t
ORIGIN      
        1 cccgatgcct tcgagcgcga cctggcgcgc gagcagaagt tcggcttcac cttcgcgccg
       61 cgcggctacc tgatcaaggc ttgagctagt cttcgatgat ctcgaagtct ttcgtgatcg
      121 cggcggtctt gccgagcatg atggatgcgg agcaatattt ctcgtgcgag agccggatcg
      181 cctgctcgac gaggttgcgc ttcagcgcgc gaccgcgcac cacgaaatgc atgtggatcg
      241 ctgtgaaaac cttcgggtcg gcctccgcgc gctcggaggt gagcctgacc tcgcagccgg
      301 tgacgttctg gccgcttttt gtgaggatca gcaccacgtc gtaggcggtg cagccgccgg
      361 tgcctgcgag caccagctcc atcgggcgcg gcgcgaggtt gcggccgcca ccctcgggtg
      421 cgccatccat ggcgacgacg tgattgcttg ctgtttcagc gagaaaagtc atgccgctgg
      481 tccagcgcac ggtgcattcc atggcagttt ccttattttt caatgcttgg tgctttgcgt
      541 cgtcaagctg ttgaaattaa gaattattta tgagagtgcg cgctcgccta tcgctgtgag
      601 gaacagtaca ttgcactgca gcaacgaccg cggcataatc ggcttgtctc ctccaccctc
      661 ctcctttggt ggattcggcg caactctagc gagcggcgcc ttttttttgt gtaaaatccg
      721 cacccttttc gcaatttcgc gcatgactac gtataccgca aaagttgggg agatcgagaa
      781 ccgctgggtg gtggtagatg cccaaggcaa ggtgctcggt cgccttgcgg cgcaaatcgc
      841 ggcgcggctg cgcggcaaac acaagcccga gtacacccca cacgtcgaca ccggcgatta
      901 cgtcgtggtg gtgaacgcgg ccaagctgcg cgtgacggga aagaaggcgg agcgcaagat
      961 ctactaccgg cattccggat tccccggcgg catcaagcag accaatttcg ccaagctgca
     1021 tgcggcgaag cccgagcgcg tgctgcagcg agcggtgaag ggcatgctgc ccaagggccc
     1081 gctcggctat gcgatgatcc gcaagctcaa gatctactcg ggtggcagcc atccgcattc
     1141 ggcccagcag ccgacaaccc tggaaatcta ggaaccgaaa atggtcggcg actattacta
     1201 cggcaccggg cggcgcaaga gcgccgtggc gcgtgtgttc ctcaagcccg gcaagggcgg
     1261 ctttgtggtg aacggcaagc cggtggacga gttcttcggc cgcgaaaccg gccgcatggt
     1321 ggtgcgccag ccgctcgagc tcaccaagag ccagtcgagc ttcgacatca tggtgaatgt
     1381 ctacggcggt ggcgaaagcg ggcaggcagg cgcggtgcgc cacggcatca cgcgcgcgtt
     1441 gatcgagtat gatgccgctc tgaagggcgt cttgagcaca gccgggctcg tcacgcgcga
     1501 tgcgcgcgag gtcgagcgaa aaaaggtcgg tctgcacaag gcacggcggc gcaagcagta
     1561 ctcgaaacgt tgaatcgaat accggacgct tcaccggatc gaaagccgcc caagggcggc
     1621 tttctcgttt ctggacacgg atacgcatga tcaaggcagg catcgtcggc ggcacggggt
     1681 acacgggggt cgagctcctg cggctcctcg tgcagcatcc gcaggtcgag ctgaaggtga
     1741 tcacctcgcg caaggaggcg ggcacaccgg tggccgccat gttttcgagc ctgcgcggcc
     1801 gcgtcgcgct cgccttcacc gagcccgacg ccaagacgct gcgcggctgc gacgtcgttt
     1861 ttttcgccac gcctcacggc gtcgccatga aagaggcgcg cacgttgctc gacggcggcg
     1921 cgaaagtagt cgacctttcc gccgatttcc ggctgcagga cctcgccgag tgggagcgct
     1981 ggtacaagat gaagcacgcc gcgcccgagc tcctgcggga agcggtgtac ggcttgtgcg
     2041 agttgaaccg tgagcgcatc cggcgcgcgc gcctggtcgc gaatccgggc tgttatccga
     2101 ccgcgatcca gctcggcttt cttccgctcg tagaagcggg cgcggtcgat ctcgaccacc
     2161 tgatcgccga tgcgaagtcg ggcgtaagcg gggcggggcg caaggccgag ctcagcctga
     2221 ccttctccga ggcctcagac aatttcatgg cctacaacgt gcccgggcat cggcaccggc
     2281 cggaaatccg cgaaagcctc gcgcaggcgg cgaagcgcga agtcggcctg gtgttcacct
     2341 cgcatctcac gccgatcatc cgcggcattc acgccaccct gtacggtcgc gtcacgaagg
     2401 aattggattt ccagcagctc ttcgagaaga ggtatgcgag tgagccattc gtggacgtga
     2461 tgccgccggg cagccatccc gacacgcgtt cggtgcgtgc ggccaacgtc tgccgcatcg
     2521 cggtccaccg gccgcccgag ggcgcgcggc aggccgacac gctggtggtg ctgtccgtta
     2581 tcgacaatct cgtcaagggg gcatccggcc aggcggtgca gaacatgaac ctgatgttcg
     2641 ggctaccgga ggccgccggc ctcggccagc tgccggtggt gccgtaatgc gcgcgttcgc
     2701 cgaccggatc gggaagctgc gccagcgctt cggcatcgcc gcgccgcgcg tcgcggtgca
     2761 ttcgcacatt gcctggtact ggcgctgggt cggagtcgcg gtgctggtcg gagtctcggc
     2821 cgcggccgcc gcctggattt acgacgcggg ccggcgcttc gccggcttcg accgcagcga
     2881 ggtgcagcag gagctcgctg ccgctctagc cgatctcgcg acggcgcgcg atgagctcga
     2941 gcggctgcgc gccatcgcca acgctgccga cagtcgcgtt tcgatcgagc gcaccgcgca
     3001 gcaaaagctc gcgcagcaaa tccgcgccct tgagcaggag aatgcgcgcg tgcgcgagga
     3061 gcttgcgacc atcgagacca tgcttagctc cgaggcgcgc tccgcggccg cgctatccat
     3121 ctaccgcttc aaagtcgagc cagatgtcct gccgggggag tatcgctatc acctgctgct
     3181 gctcactccg accacgcgcc gcgagcgcga tttcaacggt cgcctggagc tggtggtcag
     3241 cctgacggaa gggggccaga gtgttatgat gagcttcccg gaaccgagag acgccggggc
     3301 gtcggctttc cggttggcat tcaagtactt ccgtagggtg gaaggcagct tccgggtgaa
     3361 cccaaaggcg cgggtggaaa gcgtgcaggt gcgggtttac gaagcggggt cgaaccagcc
     3421 gcgcgccacg cacacggtga ccctctagcg gttagttgaa gggagaaaag cgatgttcaa
     3481 caagacgagc aagccgcaga acaggatcga cagcctgatc ggcgcgacca cgcgcatcga
     3541 gggcaacgtg atattcagcg gcggattgcg ggtggacggc atggtgcgcg gcaacgtcgc
     3601 ggcgctttcc gatcagcccg gcacgctggt gttgtctgcc gatgcccgca tcgacggcga
     3661 ggtgcaggcc gcgcacatcg tggtcaacgg cacgatcaac ggccccgttc acgccaccga
     3721 gacgctcgaa ctgcaggccg gttcccaggt caaaggcgat gtgtactaca aaagcatcga
     3781 aattcaccag ggtgcggtgg tggaagggcg cctggtgcat catccggccg acatgaaggg
     3841 cgtggagctt aagctcgcct cgggcggata acagggaaga cggactcagg agctcatgca
     3901 atgaacgcag tgaccgaatc agttaccgag atgccagcgc cgctggtgtt caccgacaac
     3961 gccgcgacca aggttaagca gctgatcgac gaggaaggca acccggagct caagcttcgc
     4021 gtgttcgtca ccggcggcgg ctgctcgggc tttcagtacg gctttacctt cgacgaaatg
     4081 cagaacgagg atgacgccgt gatggagaag aacggcgtca agctgctcat cgacccgatg
     4141 agctaccagt acctcgtggg ttccgagatc gattaccaag aaggtctcga aggcgcccag
     4201 ttcgtgatca agaaccccgg cgcgaagagc acctgcggtt gcgggtcgtc tttctcgacc
     4261 tgaattcgaa aaaactctga acgaagcaca aagcgaggac ctcgcgcaat ggcgtacgcg
     4321 cgcggcggcc ggcgatgtgg cagcgaaagc ggcgctcggg cgccatctcc tgacgcaaac
     4381 gccgggcgac atgcaagaag gcgtcgagtg gaccgctgcc gcggcgaagg aaggtagcgg
     4441 cgaagcggcg tacctcttgg cggtgctggc ctcctccggc atcggcgtcc cgcaggatct
     4501 gaaagcatcg ctgcattatt tgcagcaggc cgcggaacgc ggccatcggc cggcgcaaac
     4561 cgagctcgcc gcattggtcg gcaactggcg cctcgtgcgc gagatttcct ccggaaaaat
     4621 tccccgacag gacacctggg atcgcttgcg cgcggcggtc gatacggagg cgtggttgag
     4681 cgtgccccat ggaagcgtgt tctcggcgga gccgcgcatc gcggcggtga agggctttat
     4741 ttcagctcac atctgcgatt ggctggtgcg gcttggtaag ccgcatttgc agcaggcgga
     4801 aatatatgac actgagagcg gagacctgcg cgaagacggc cggcgcagca acagtgcaac
     4861 cgggctcggg ctcgcccgca tggacatggt gctcgccttc gtgcgtgcgc gcatcgcggc
     4921 gctggccgag ttgccggtct ttgcgttaga gaccagccag gttctacact atgaagtggg
     4981 acagcagttc agcccgcacc atgatttcct ggacgtgagt tttcctggtc ttgcgcgcaa
     5041 agtcgcaagc gaaggccagc gcgcgctcac actgctcatc tacttgaacg aggattacga
     5101 gggcggagat accgcgtttc cgacgctcgg ccgcagtttc aaggggcgca agggcgacgc
     5161 gctgattttc tggaacgtaa cggaagacgg cgcgccagac tggcgcactc tgcacgtagg
     5221 gacggcaccg accagcggtg agaagtggct tttttcgcaa tggattcgaa ttcgcgcggc
     5281 ttaaccggcg cgcgtaaccg ggattcggct cagtcgagca gtgcgagatt ggtgtcggcg
     5341 agcagatcca gccgcgcgat cagcggcgcg gcatggatcc ggaactcggg cagctgctgc
     5401 gccgggacgg gctgagccgc cggcatcgcg atcgacagcg ggttgcgcgc ctggccggcg
     5461 acctggaact cgtagtgcag gtgcggcccg gtggcccagc cagtccggcc gacgaagccg
     5521 atggtgtcgt tctgcgccat gcgcgcgccg cgatgcagcc ccgccggaaa gcggctgaga
     5581 tgcgcataca gggtggcgta ctgaccgtgg tggcgcagca ctaccgcctt gccgtaaccg
     5641 cctttctcgc cgacgaactc cacgatgccg tcgcctaccg cgcgcacgcg cgtgcctgcc
     5701 gcggccacgt aatcgatgcc cttgtgcgct cgccaggtgc ggaatatcgg atgcacccgc
     5761 aggccgaagc ccgatctcac gcgcgcgaag tccagaggcg agcgcagg
//
LOCUS       DASPXW010000184         3523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_303883, whole genome shotgun sequence.
ACCESSION   DASPXW010000184 DASPXW010000000
VERSION     DASPXW010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303883"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..757)
                     /locus_tag="VEL04_04355"
     CDS             complement(8..757)
                     /locus_tag="VEL04_04355"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HYT47628.1"
                     /translation="MMDQLRGKVAIITGAGGGICRAIATTFAQAGAKVACLDMNLSNA
                     EETARLAGQGALAIGCDVSSESDTKAAVERAAKAFGRIDILVNGAAMRDPTATVLDLD
                     LAAWNKVFATNVGGAFLMSRWAIPHMLAAGGGSIIHIASQLGTVGTPGRVAYCSTKGA
                     LITMAKAMAADHAAQGIRVNTLSPGGVETERMLRFGSMEKAREVLGSKHLMQRLGQPE
                     EIAQAALFLASNASSFMTGSDLRVDGGYNAV"
     gene            complement(754..1464)
                     /locus_tag="VEL04_04360"
     CDS             complement(754..1464)
                     /locus_tag="VEL04_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT47629.1"
                     /translation="MLKVQNVELAYGEVPAVRDISFEVAEGEIVTLIGANGAGKSTTM
                     RGVAGAMLPRRGRIVFDGRDVTRMPAHARAAAGIALVPEGRRVFPALTVRENLEMGGF
                     KYRRDRGKVNALIERAMQSFPQLRERASQPAGTLSGGEQQMLALSRALMSEPRLLCMD
                     EPSLGLAPIIVQDIFRSIRTVNASGTSILLVEQNARYALETASRGYVLQTGSIIAAGA
                     CADLKQDERVKEAYLGRK"
     gene            complement(1457..2209)
                     /locus_tag="VEL04_04365"
     CDS             complement(1457..2209)
                     /locus_tag="VEL04_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006396014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT47630.1"
                     /translation="MSLLRAERLNKSFGGVAAVQNVSIHVPQGAIFALIGPNGAGKST
                     LLNLLLGIYQPDSGSLSFGDSNLIGLPAHERVRLGIARTFQKIRLFKQLTLLENVLAG
                     FHIHQDVPLLKCFMPGGVGRFRDEAMELLSFVGLGNQANDRAGALPYGQQRMLEIARA
                     LATRPKLFMLDEPAAGLNAAEVDFLLKRLENINKRGVTVVVVEHNMDLVMNVAQRVFV
                     MDHGVALFEGTPGEVQANPRVVEAYLGGEFAA"
     gene            complement(2206..3105)
                     /locus_tag="VEL04_04370"
     CDS             complement(2206..3105)
                     /locus_tag="VEL04_04370"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYT47631.1"
                     /translation="MAWVVSLILVAAAPWLLTTGYWRGILVLCAMNVLLALSLNLVMG
                     YTGQLNLGQSAFFGIGAYVSTILIKSYGWNFWLAALVAVGAAGLVGVALAAFAVRLRG
                     HYLAIASLGFAVITYQVLVNWESVTQGVRGMYGILPPVPNIVALFYLVAAVAFVIYFL
                     LDNLVRSPVGDTLRAIREDEISAASLGVNSAVWKAFAFGIGAAIAGLAGVFYPGFVGT
                     LVPDAFGIVESFTMMAMVIVGGMGTMVGPVLGAIALTFLPELLRGFGELRLMIYGLAL
                     TLVVLFMPGGMVQAWISLRRRQG"
     gene            complement(3117..>3523)
                     /locus_tag="VEL04_04375"
     CDS             complement(3117..>3523)
                     /locus_tag="VEL04_04375"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYT47632.1"
                     /translation="FMRFTFLGKALRAVAQDRETAGLMGINESLVIALTIVIASALAG
                     VAGALLGPVVVLTPFTGASLIVKAFACVIIGGFGNMEGTIIAGLLVGLIEAFTVQYLG
                     TGIIDLVVFAMLLVMLVVRPTGLIAERKEENV"
BASE COUNT          633 a         1154 c         1208 g          528 t
ORIGIN      
        1 ctgcaggtca gaccgcgttg taaccgccgt cgacgcgcag atccgagccg gtcatgaagc
       61 tcgaggcgtt ggaagcgagg aagagcgcgg cctgggcgat ctcctcgggc tggccgaggc
      121 gctgcatgag gtgcttggag ccgagcactt cgcgcgcctt ctccatcgag ccgaagcgca
      181 gcatgcgctc ggtctccacg ccgcccggcg acagagtatt gacgcggatg ccctgcgcgg
      241 cgtggtcggc ggccatggcc ttggccatgg tgatgagcgc gcccttggtc gagcagtagg
      301 cgacgcgtcc cggggtgccg acggtgccga gctgggaggc gatgtggatg atcgagccgc
      361 cgccggcggc gagcatgtgc gggatcgccc agcggctcat gaggaaggcg ccgccgacgt
      421 tggtggcgaa gaccttgttc caggctgcga ggtcgaggtc gagcaccgtg gcagtgggat
      481 cgcgcatcgc ggcgccgttc acgaggatgt cgatgcggcc gaaggctttc gccgcgcgct
      541 cgaccgccgc cttggtgtcg gattcggacg agacgtcgca gccgatcgcg agcgcgccct
      601 gcccggcgag ccgcgccgtc tcctcggcgt tactgagatt catgtcgaga caggcgactt
      661 tcgcgccggc ctgggcgaac gtggtcgcaa tcgcgcggca gatgccgccg ccggcgccgg
      721 tgatgatcgc gaccttgccg cggagctgat ccatcatttc ctccctaaat aagcttcctt
      781 cacgcgttca tcctgcttca ggtccgcgca ggcgccggct gcgatgatgc tgcccgtctg
      841 cagcacgtag ccgcggctcg cggtctcgag tgcatagcgc gcgttctgct cgacgagcag
      901 gatgctcgtg cccgaggcgt tgaccgtgcg gatgctgcgg aagatgtcct gcacgatgat
      961 cggcgcgagg ccgagcgagg gctcgtccat gcacaacagg cgcggctcgg acatgagcgc
     1021 gcgcgagagc gcgagcatct gctgctcgcc gcccgagagc gtcccggccg gctgcgaggc
     1081 gcgctcgcgc agttgcggaa agctctgcat ggcgcgctcg atgagcgcat tgaccttgcc
     1141 ccggtcgcgg cggtacttga agccgcccat ctcgaggttc tcgcgcacgg tgagcgcggg
     1201 aaacacgcgc cggccttcgg gcacgagcgc gatgcccgcc gcggcgcgcg cgtgcgccgg
     1261 catgcgcgtg acgtctcggc catcgaacac gatccggccg cggcgcggca gcatggcgcc
     1321 ggcgaccccg cgcatggtgg tgctcttgcc cgcgccgttg gcgccgatga gcgtcacgat
     1381 ctcaccttcg gcgacttcga aggagatgtc gcgcacggcg ggcacctcgc cgtaggcgag
     1441 ctccacgttt tgtactttaa gcagcaaatt cgccgcccag ataggcctcg accacgcgcg
     1501 ggttcgcctg cacctcgccc ggcgtgccct cgaagagggc gacgccgtgg tccatgacga
     1561 acacgcgctg cgccacgttc atcacgagat ccatgttgtg ctccaccacg accaccgtga
     1621 cgcctcgctt gttgatgttt tccagccttt tcagaagaaa atcgacttcg gcggcgttca
     1681 ggcccgccgc cggctcatcc agcatgaaga gcttcggccg cgtggcgagc gcgcgcgcga
     1741 tctcgagcat gcgctgctgg ccgtaaggga gcgccccagc ccgatcgttg gcctgatttc
     1801 caaggccgac aaacgagagg agctccatcg cctcgtcgcg gaagcgtccc accccgccgg
     1861 gcatgaagca tttcagaagc ggcacgtcct ggtggatgtg aaagcccgcg agcacgttct
     1921 cgagcagcgt gagctgcttg aagaggcgga tcttctggaa ggtgcgcgcg atccccagcc
     1981 gtaccctctc atgcgccgga agaccgatca ggttcgagtc accaaatgaa agagaacctg
     2041 aatcaggctg gtagataccc aagagcaggt tgaggagcgt cgacttgccc gcgccgttgg
     2101 gaccgatgag cgcgaagatc gcgccctgcg gcacgtggat cgaaacgttc tgcacggccg
     2161 ccacgccgcc gaagctcttg ttcaggcgct cggcgcggag gaggctcacc cctgccttct
     2221 ccggagagaa atccatgcct gcaccatccc ccccggcata aaaagcacca cgagcgtgag
     2281 cgcgaggccg tagatcatga ggcgcagctc gccgaagccg cgcaggagct ccggaagaaa
     2341 ggtaagcgcg atggcgccga gcaccggccc caccatcgtg cccatgccgc cgacgatcac
     2401 catcgccatc atggtgaaag actcgacgat gccgaaggcg tcgggcacga gcgtgccgac
     2461 gaagcccgga tagaacacgc cggcgagccc ggcgatcgcc gcgccgatgc cgaaggcgaa
     2521 ggctttccac accgccgagt tcacgccgag agaagccgcc gagatctcgt cctcgcggat
     2581 cgcgcgcaag gtatcgccga ccggcgagcg caccaggttg tcgagcagga aatagatgac
     2641 gaaggcgacc gcggcgacga gatagaagag cgccacgatg ttcggcaccg gaggcaggat
     2701 gccgtacatg ccgcgcacgc cctgcgtcac gctctcccag ttgacgagca cctggtaggt
     2761 gatgaccgcg aagccgagcg aggcgatcgc gaggtagtgg ccgcgcaggc gcacggcgaa
     2821 agcggcgagc gccacgccga cgaggcccgc ggcgccgacc gcgacgagcg ccgcgagcca
     2881 gaagttccag ccgtagctct tgatgaggat ggtcgagacg taggcgccga tgccgaagaa
     2941 cgccgactgg ccgaggttga gctggccggt gtagcccatc acgaggttga gcgacagcgc
     3001 gagcagcacg ttcatcgcgc acagcacgag gatgccgcgc cagtaaccgg tggtgagcag
     3061 ccatggggcc gcagcgacca gaatcaagct gactacccat gccattcttc tttgcgtcaa
     3121 acgttctcct ccttgcgctc ggcgataagg ccggtggggc gcaccaccag catgacgaga
     3181 agcatggcga agactacgag gtcgatgatt cctgtgccga gatactgcac ggtgaaggcc
     3241 tcgatcaggc cgacgagcag cccggcgatg atggtgccct ccatgttgcc gaagccgccg
     3301 atgatcacgc aggcgaaggc cttgacgatg agcgaggcgc cggtgaacgg ggtgagcacc
     3361 accaccggcc cgagcagcgc gccggcgacg ccggcgagcg ccgaggcgat cacgatggtg
     3421 agcgcgatca ccaggctctc gttgatgccc atgaggccgg cggtctcgcg gtcctgcgcc
     3481 accgcgcgca gcgccttgcc gaggaacgta aagcgcatga aag
//
LOCUS       DASPXW010000185         9799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_934531, whole genome shotgun sequence.
ACCESSION   DASPXW010000185 DASPXW010000000
VERSION     DASPXW010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9799
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934531"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1416)
                     /gene="lptD"
                     /locus_tag="VEL04_04380"
     CDS             complement(<1..1416)
                     /gene="lptD"
                     /locus_tag="VEL04_04380"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HYT47633.1"
                     /translation="MPYRFATLAVALVAAAPALAQTAGKDAMGATTIDAETIEGVGDL
                     EVTARGRAEIRRDDLSIFGEELRFNREFGRLTGEGGVRLQSGADRFFGPRLQYNTADD
                     SGVFEQPGYLLERERTARGGAERIEFLGKGRYRLVNGTFTTCQPGQEDWRLEADELEL
                     DYDTDEGRARYPRFRFFDHTVLAAPYISFPLESRRKSGLLTPYYSQNSRRGLEVGIPY
                     YWNIAPELDATLTPVYMAKRGEQLKNQVRYLQSSYAGELKLEYLPNDKEFGESRRGVS
                     LQHAQTIRPNLTAQVDYNKVSDNRYFVDLASQVRQVSLRTLPQDGYVTYANPALGSGT
                     TLQARMQRFQTLQDPLAPIVPPYDRLPQFNLISGKGDLGRVLDGGFSAEFVRFRHETL
                     VEGSRYSANPSLAMPLLGPGWFITPKAGMRYAGYSLDRATDGQDAAPRVAIPWLSIDN
                     GLVFERDAHFLGNNVTQTLEPR"
     gene            1452..2429
                     /locus_tag="VEL04_04385"
     CDS             1452..2429
                     /locus_tag="VEL04_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HYT47634.1"
                     /translation="MIHADTRRAALSGWLAAQLRAARFSLEPASEDASFRRYWRARLE
                     DGRSFVVMDAPPDKEDCRPFVRVAGLLRAAGVHAPEIFAQDHGLGFLLLADLGTTPYL
                     EALTADNAARLFADATEALLRWQLATRQGELPPYDEALLRREMNLFPEWYIAQHLKID
                     LSLDQKRSLENIFALLAKDALAQPRVYVHRDYMPRNLMVCEPNPGVLDFQDAVLGPIT
                     YDMVSLLRDAFISWDEERVLDWGVRYWEKAKRAALPVMADFAEFWRAFEWMGLQRHLK
                     VLGIFARIRYRDGKPKYLEDTPRFIGYARAVATRYRELAPLARLLDEIE"
     gene            2426..3109
                     /locus_tag="VEL04_04390"
     CDS             2426..3109
                     /locus_tag="VEL04_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HYT47635.1"
                     /translation="MKAMILAAGRGERLRPLTDTLPKPLVRAGGKALIAWHLERLAAA
                     GCRDAVINVSHLGDRIIEHLGDGARYGLHIAYSREREPLETAGGIAQARGLLGTDPFL
                     VLNGDIYCEVDVARLLGVTLVERLAHLVLVPNPPHHPAGDFTLERGVIGNAATARYTY
                     AGVAVISPALVAGVQPGGKAQLAPLLRAAAEKGLISGELYTGIWQDAGTAERLAKLEG
                     QLASRNENR"
     gene            3096..4421
                     /locus_tag="VEL04_04395"
     CDS             3096..4421
                     /locus_tag="VEL04_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004547630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HYT47636.1"
                     /translation="MKIAEDALLYRKRRERLARAMQAGIAVIPTAPERVRNRDSHYPY
                     RFDSHFYYLTGFAEPGAALVIVAGEAPRSLLFCRTRSEEREIWDGFRYGPEAARERFG
                     FDAAHPIEALDEALASLLENQPALYYPVGDDPEWDARAMKWLNAVRARVRAGVAAPDR
                     VQDVRALVDDMRVVKDPHEQLLMRRAARIAAAAHRRAMQCVRPGRNEYEIEAELLYEF
                     RRSGAQFPAYSPIVAGGANACVLHYVFNDAPLRDGDLLLIDAGCELDGYASDITRTFP
                     VNGRFSGAQREVYELVLAAQHAAIEKVRTGSEWIAPHDAAVRVLAQGMLDLKLLAGTL
                     DEVLEKESYKRFYMHRTGHWLGLDVHDAGDYRRSGKWRALAPGMTLTVEPGLYIRAAD
                     DIPERLRNLGVRIEDDVLVTASGCEILTAEAPKSVDDIEALMRDARARH"
     gene            4402..5454
                     /locus_tag="VEL04_04400"
     CDS             4402..5454
                     /locus_tag="VEL04_04400"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HYT47637.1"
                     /translation="MPERGIDVLIRGAGPVGCTLALGLRGSGLEVAVLDVSTAKSSFR
                     PIALSYASRLILERIGAWQGLASTPIETIHVSQRGGFGQTRLEAADAGVPALGYVTDY
                     SNLAGILRSRVQPLITQDEIAARCVVHAEGSSVQAEEKRYAQDAVVALVTVDPSSRSV
                     AFERFTADGPLALLPLGGRYALVWSTRPEKARALVEAPEPQFLAELAAAAGGRPGRPL
                     QAEARMTLPLVLRVRATRVAPREVFIGNAAQTLHPVAGQGLNLGLRDAWDLAEAWRAG
                     GDPGDAAMLARYAARRRADAAATIRLTDFLAGAFLGTSRLGRGARGAAMAMLDIFPPA
                     RRFFARRMIYGSSALP"
     gene            5524..6477
                     /gene="dusB"
                     /locus_tag="VEL04_04405"
                     /pseudo
     CDS             5524..6477
                     /gene="dusB"
                     /locus_tag="VEL04_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156395.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
     gene            6489..6728
                     /locus_tag="VEL04_04410"
     CDS             6489..6728
                     /locus_tag="VEL04_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYT47638.1"
                     /translation="MSRSNELADCVKRSLERYFKDMDGEKPTSIYEMVLKNIEKPMIE
                     TVLGKASGNQSLAAEMLGVTRNTLRKKMQQLRIKG"
     gene            6728..8284
                     /gene="purH"
                     /locus_tag="VEL04_04415"
     CDS             6728..8284
                     /gene="purH"
                     /locus_tag="VEL04_04415"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014617933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HYT47639.1"
                     /translation="MTKVSRALLSVSDKSGIVEFARALAALGVELISTGGTAQLLAKE
                     SIAVTEVSAYTGYPEMLDGRVKTLHPKIHGGLLARRDDPAHLAALKSAGIAPIDLVAV
                     NLYPFQATVADPDCSFDAAVENIDIGGPALLRAAAKNHGAVTVVVDPADYPRVLEELR
                     RGGISDATRFAFARKVFAHTAGYDGAIANYLSSLDASRRRREYPDVLTLQFAKLQDLR
                     YGENPHQSAAFYRDERAVAGSLATYRQLQGKELSYNNIADADAAWECVKSFPEPACVI
                     VKHANPCGVATGQNLLAAYQKAFKTDPVAAFGGILAFNRALDQAAAQALGKQFAEVVI
                     APRIEADAAHELSKKTNLRLLEAPLSHDVQRHDFKRVGGGLLVQTTDSRVLKKEDLKV
                     VTKKQPSEAQWSDLLFAWRVAKFVKSNAIVFCRDGMTLGIGAGQMSRVDSSRLAAMKA
                     QAAKLPLAGAAVASDAFFPFRDGLDVVLEAGATAVIQPGGSARDEEVIAAADARAVAM
                     VFTGVRHFKH"
     gene            8307..9581
                     /gene="purD"
                     /locus_tag="VEL04_04420"
     CDS             8307..9581
                     /gene="purD"
                     /locus_tag="VEL04_04420"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HYT47640.1"
                     /translation="MKLLVIGSGGREHAIAWKLTQSPRVQKVFVAPGNAGTALEPGLE
                     NVALTAISDLISFSKQKEIQLTVVGPEAPLAAGIVDAFRDAGLRIFGPTRAAAQLETS
                     KDFAKAFMVRHGIPTARYATFARAGEAKAHVEKHGAPIVIKADGLAAGKGVVVAATRE
                     EAHAAIDLMLEDKRLGAAGSRVVIEECLQGEEASFIVMSDGVNVLALATSQDHKRLCD
                     GDAGPNTGGMGAYSPAPVVSPEVHSRVMREIILPAVQGMAKDGLPYAGFLYAGLMIDR
                     SGNPRTLEFNCRLGDPEAQPIILRLKSDLFELLEHALAGTLDRVEANWDRRSALGVVL
                     AAHGYPDEPRKGDRVEGLPPPSEDCRVFHAATRLEGKSVLTNGGRVLCVTALGDSVKM
                     ARSRAYEAVEHIGFDGMQYRKDIGHRALKKHP"
     gene            9578..>9799
                     /locus_tag="VEL04_04425"
     CDS             9578..>9799
                     /locus_tag="VEL04_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HYT47641.1"
                     /translation="MTPRAQAVHEYFAELQARIVAALEALDGTSFGSDSWQRPESGGG
                     ITRVVEQGRVFERGGVNLSRVQGRELPPSA"
BASE COUNT         1623 a         3280 c         3323 g         1573 t
ORIGIN      
        1 ccgcggctcg agggtctgcg tcacgttgtt gcctagaaag tgcgcatcgc gctcgaacac
       61 caggccgttg tcgatgctca gccaggggat ggcgacgcgc ggcgcggcgt cctgcccatc
      121 agtggcccga tcgaggctgt agccggcgta gcgcatgccg gccttcggcg tgatgaacca
      181 gccggggccg agcagcggca tcgcgagcga aggattggcg ctgtagcgcg agccctcgac
      241 cagggtttca tgcctgaagc ggacgaactc ggcgctgaag ccgccgtcca ggacgcggcc
      301 gaggtcgccc ttgccgctga tcagattgaa ctgcggcagc cggtcataag gcggcacgat
      361 cggcgcgagc ggatcctgca gggtctggaa gcgctgcatg cgcgcctgca gggtggtgcc
      421 ggagccgagc gccggattcg cgtaagtcac atagccgtcc tgcggcagcg tgcgcagcga
      481 cacctgcctc acctggctcg cgaggtcgac gaaatagcgg ttgtcggata ccttgttgta
      541 atcgacctga gccgtcaggt tcggacggat cgtctgcgcg tgctgcagcg atacgcccct
      601 tctcgattca ccgaattcct tgtcgttggg caggtattcg agcttcagct cccccgcata
      661 cgagctctgg agataacgca cctgattctt gagctgctcg ccgcgcttcg ccatgtacac
      721 cggggtaagc gtcgcgtcga gctcgggcgc gatgttccag taatacggaa ttccgacttc
      781 caggccgcgc cggctgtttt gcgagtaata cggcgtgagc aatccgctct tgcgccggct
      841 ttcgagcgga aagctgatgt acggcgccgc gagcacggtg tggtcgaaga agcgaaaacg
      901 cgggtagcgc gcgcggcctt cgtccgtgtc atagtcgagc tccagctcgt cggcctcgag
      961 gcgccagtcc tcctgcccgg gctgacaggt ggtgaaggtc ccgttcacca gccggtagcg
     1021 ccccttgccg agaaattcga tgcgctcggc gccgccgcgc gccgtgcgct cgcgctccag
     1081 caggtagccg ggctgctcga acacaccgct gtcatccgca gtgttgtact gcagccgcgg
     1141 cccgaagaag cggtcggcgc cgctctgcag ccgcaccccg ccctcgcccg tcaggcggcc
     1201 gaactcccgg ttgaagcgca gctcctcgcc gaagatgctc aggtcatcgc gccggatttc
     1261 cgccctgccg cgcgccgtga cctcgaggtc gcccacgccc tcgatcgtct cggcgtcgat
     1321 ggtcgtcgct cccatggcat ccttgccggc ggtttgcgcg agggctggcg cggcggcaac
     1381 gagcgcgacg gccagcgtcg caaagcggta cggcatcgag cgacgaagga tctttttagt
     1441 tagaattttc aatgatacac gccgatacgc ggcgcgcagc cctgagcggc tggctggctg
     1501 cgcaattgcg cgccgcgcgc ttctctctag aaccggcatc cgaggacgcc agcttcagac
     1561 gctattggcg cgcgcgcctg gaggacggcc gcagcttcgt cgtcatggac gcgccgcccg
     1621 acaaggagga ttgccggccg ttcgttcgcg tcgccggcct gctgcgtgcc gccggcgtgc
     1681 acgcccccga gatcttcgcg caggatcacg gcctcggctt tctgctgctc gccgatctcg
     1741 gcacgacgcc ctacctcgag gcgctgaccg cggacaacgc ggcgcggctt ttcgccgatg
     1801 ccaccgaggc gctgctgcgc tggcagctcg ctacccgcca aggcgagctg ccgccttacg
     1861 acgaggccct gcttcgccgc gagatgaatc tcttccccga gtggtatatc gcgcagcatt
     1921 tgaaaatcga tctctctctt gatcagaaaa gatcgcttga aaacatcttt gccttgctcg
     1981 ccaaggacgc gctggcgcag cccagggtct acgtgcaccg cgactacatg ccgagaaacc
     2041 tgatggtgtg cgagcccaac ccgggcgtgc tcgacttcca ggacgcggtg ctcggtccga
     2101 tcacgtacga tatggtttcg ctgctgcgcg acgctttcat cagctgggat gaggaacggg
     2161 tgctcgactg gggcgtgcgc tactgggaaa aggcgaagcg cgcggcactg ccggtgatgg
     2221 cggacttcgc ggagttctgg cgcgcattcg agtggatggg gctgcagcgc cacctcaagg
     2281 tgctcggtat cttcgcccgc atccgctacc gcgacggcaa gccgaagtat ctcgaggaca
     2341 cgccgcgctt catcggctac gcgcgcgcgg tcgcgacccg ctaccgcgaa ctcgcgcctc
     2401 tcgcgagact gctcgacgaa attgaatgaa ggcgatgatc ctggctgccg ggcgcggcga
     2461 gcgcttgcgt ccgctaaccg acacgctgcc caagccgctg gttcgggcag gaggcaaggc
     2521 cctgatcgca tggcatctcg agcgccttgc cgcggccggc tgtcgcgacg cggtgatcaa
     2581 tgtctcgcac ctcggcgatc gcatcatcga gcacctcggc gacggcgccc gttatggcct
     2641 gcacatcgcc tactcgcgcg agcgcgagcc gctggagacc gcgggcggca tcgcgcaggc
     2701 gcgcgggctc ctgggaacgg acccttttct ggttctcaac ggcgacatct actgcgaggt
     2761 cgacgtagcg cgcctgctcg gcgtgacact ggtcgagcgg ctggcgcacc tcgtgctcgt
     2821 gcccaatccg ccgcatcatc ctgccggaga cttcacgctc gagcgcggcg tgatcggcaa
     2881 cgccgcaaca gcgcgctaca cttatgcagg cgtggccgtc atctcgccgg ccctcgtcgc
     2941 aggcgtgcaa cccggcggca aggcgcaact ggcgccgcta ttgcgcgccg cggccgagaa
     3001 agggctgatc agcggcgagc tttacaccgg aatatggcag gacgcgggta cggccgaacg
     3061 actcgccaag ctcgaggggc aactggcatc gcgaaatgaa aatcgctgaa gacgcattgc
     3121 tctacaggaa gcgccgcgag cggctggcgc gggccatgca ggcggggatc gcggtcatcc
     3181 cgaccgcacc cgagcgcgtg cgcaaccgcg actcgcacta cccgtaccgc ttcgacagcc
     3241 acttttatta tctgacgggc ttcgccgagc ccggcgccgc cctggtcatc gtcgccggcg
     3301 aggcgccgag gtcgctgctt ttttgccgca cccgcagcga ggagcgagag atctgggacg
     3361 ggtttcgcta cggtcccgag gcggcgcgcg agcgcttcgg tttcgacgcc gcgcacccga
     3421 tcgaagcgct cgacgaggcg ctcgccagcc tgctcgagaa tcagcccgcg ctctattacc
     3481 cggtcggcga cgacccggaa tgggacgcgc gcgccatgaa gtggctgaat gcggtgcgcg
     3541 cccgcgtgcg cgcgggcgtc gccgcgcccg atcgtgtcca ggacgtgcgc gcgctggtcg
     3601 acgacatgcg cgtggtgaag gacccgcacg agcagttgct catgcggcgc gcggcgcgta
     3661 tcgccgcggc ggcgcaccgc cgggccatgc agtgcgtgcg gcccggacgc aacgagtacg
     3721 agatcgaggc agagctgctg tatgaattcc gccgcagcgg cgcgcagttt cccgcctact
     3781 cgccgatcgt cgcgggcggc gccaatgcct gcgtgctgca ctacgtgttc aacgacgcgc
     3841 cgctgcgcga cggcgatctg ctgctgatcg atgccggctg cgagctcgac ggctacgcct
     3901 cggacatcac gcgcaccttc ccggtgaatg gacgcttcag cggcgcgcag cgcgaggtct
     3961 acgagctggt gctcgccgcg caacacgcgg caatcgagaa ggtgcgcacg ggaagcgagt
     4021 ggatcgcgcc gcacgacgcg gcggtacgag tgctggcgca gggcatgctc gacctcaagc
     4081 tgcttgccgg gactctcgac gaggtgctcg agaaggagag ctacaagcgc ttctacatgc
     4141 atcgcaccgg gcactggctc gggctcgacg tgcacgacgc aggcgactat cggcgctcgg
     4201 gcaaatggcg cgcgctggcg cccggcatga cgctcaccgt cgagcccggc ctgtacatcc
     4261 gcgcggcgga cgacatcccg gaacggctgc gcaatctcgg cgtgcggatc gaggacgacg
     4321 tgctcgtcac ggcgagcggc tgcgaaattc taaccgccga ggcgccgaag tcggtggacg
     4381 acatcgaggc gctgatgcgc gatgcccgag cgcggcattg acgtactgat ccgcggcgcg
     4441 gggccggtcg gctgcacgct cgccctcggc ctgcgcggct cgggtctgga agttgccgtc
     4501 ctcgatgttt caactgcaaa atcctctttc aggcctatcg cgctttcgta tgcgagccgc
     4561 ctgatcctcg agcgcatcgg cgcatggcag gggctcgcgt cgacgccgat cgaaacgatc
     4621 cacgtatcgc aacgcggcgg gtttgggcaa acgcggctcg aggcggccga cgcgggcgtt
     4681 ccggcactcg gttatgtaac tgattattcg aatctggccg gcattttgcg aagccgggta
     4741 cagcccttga ttacacaaga tgaaattgca gcgcgctgcg tcgtccacgc cgagggctcg
     4801 tcggtgcaag ccgaagaaaa gcgttacgcg caggacgccg tcgtggcgct cgtgacggtc
     4861 gacccgtcct cgcgcagcgt ggcgttcgaa cgcttcaccg cagacggccc gctcgcgctg
     4921 ctgccgctcg gcggccgtta cgcgctcgta tggagcaccc ggccggagaa ggcccgggcc
     4981 ctggttgagg cgccggaacc acagttcctt gccgagctcg cggccgccgc cggcggccgg
     5041 cccggccggc cgctgcaggc cgaggcgcgc atgacgctgc cactcgtgct gcgcgtgcgg
     5101 gcgacgcgcg tcgcgccgcg cgaggttttc atcggcaacg cggcgcagac gctgcatccg
     5161 gtcgccgggc aggggctcaa cctcggcctg cgcgacgcct gggaccttgc cgaggcctgg
     5221 cgcgcgggcg gcgacccggg cgacgccgca atgctcgcgc gctacgccgc gcgccggcgc
     5281 gcggacgccg ccgcgaccat ccggctcacc gattttctcg ccggcgcatt cctcggcacg
     5341 agccgcctgg gtcgcggcgc acgcggcgcg gcgatggcca tgctcgacat ttttcccccc
     5401 gcgcggcggt tttttgcacg ccgcatgatt tacggatcct ccgcgctgcc ctgaaaaaaa
     5461 ttctggattc tttttcgcgg tgaaacgagc tagaatccgc ttccctccaa cgtcaaccac
     5521 gaaatgagaa tcggcgcgca tcaactgcgc aacgggttgt tcgtcgctcc gatggcgggg
     5581 atcaccgatc gcccgttccg gcgccttgcc aggcgctatg gcgccgcgct cgcggtctcc
     5641 gagatggtgt cgtcgaggcc cgagctgcgt gaatctcgca agacccgcct cagggtggac
     5701 cacgctggcg agcccggccc gatttcggta cagatcgcag gcagcgatcc gcgcctcctc
     5761 gccgatgccg cccgcttcaa cgtcgagcgc ggcgccgaaa tcatcgacat caacatgggc
     5821 tgcccggcca ggaaggtatg caatgtcgca gccggctcgg cgctgctcga ggacgagccg
     5881 cgggtggcgc gcatcctcga agcggtggtc gccgcagttg atgttcccgt tacgctcaag
     5941 atccgcaccg gacccggtcc ggaccggcgc aatgccgtgc gcatcgcgcg gctcgccgaa
     6001 gcagccggcg tgcagctgct cgccatccat ggccgcacgc gcgcctgcat gttcgagggc
     6061 cgcgccgaat acgacaccat cgcggaagtg aaatcgcggt tgcgcatccc ggtggtcgcc
     6121 aacggcgacg tgcgcacgcc cgaggacgcg aagcgcgttc tcgaggcgac cggcgccgac
     6181 ggcatcatgg tgggacgcgc ggcgcagggc cggccgtggc tctatcgcga gatttcgcac
     6241 tacctcgaga ccggcgagcg gctggcgccg ccgccggctg ccgagatggc agcagtgctc
     6301 atcgagcatc tcgaggggct gtacgcgctg tacggggcgg agcagggggc ccgggtagcg
     6361 cgcaagcaca tcggctggac ggtgcgcgag ctgcccggag gggaggcgct gcgctgcgcg
     6421 gtaaacacga tcgacgccgc ggcggcgcag tgcgcagcag tgaacgacta tttcgagaga
     6481 ctcgccgcat gagccgcagc aatgagctcg cagactgcgt caagcgctcg ctcgagcgct
     6541 acttcaagga catggacggc gagaagccga cctcgatcta cgagatggtg ctgaagaaca
     6601 tcgagaagcc gatgatcgag acggtgctcg gcaaggcctc gggcaaccag tcgctcgccg
     6661 ccgagatgct cggcgtgacc cgcaacacgc tgcgcaagaa aatgcagcag ctgcgaatca
     6721 aagggtagtg acgaaggtca gccgggcgct tctcagcgtc tccgacaagt cgggcatcgt
     6781 cgagttcgcc cgcgcgctcg ccgcgctcgg cgtcgagctg atttccaccg gcggcacggc
     6841 gcagctgctc gccaaggaat ccatcgcggt aaccgaggtc tcggcctata cgggatatcc
     6901 cgagatgctc gatggccggg tgaagacgct gcatcccaag attcacggcg gcctgctcgc
     6961 gcgccgcgac gatcccgcgc atctcgcggc cctgaagagc gccggcatcg cgccgatcga
     7021 tctcgtcgcg gtgaacctct atccattcca ggcgacggtg gccgatcccg actgcagctt
     7081 cgacgcagcg gtcgagaaca tcgacatcgg cggacccgcg ctgctgcgcg cagcggccaa
     7141 gaaccatggc gcagtcacgg tggtggtcga cccggccgac tacccgcgag tgctcgagga
     7201 gctgcgtcgc ggcggcatct cggacgccac gcgcttcgcg tttgcgcgca aggtgttcgc
     7261 gcataccgcc ggctacgacg gcgcgatcgc gaactatctt tcttcgctcg atgccagccg
     7321 gcggcggcgc gagtatcccg acgtgctcac gctgcagttc gcgaagcttc aggacttgcg
     7381 ttacggggag aacccacacc agagcgcggc tttctaccgc gacgagcgcg cggtcgccgg
     7441 cagcctggcg acttaccgac agctgcaggg caaagagctc tcctacaaca acatcgccga
     7501 cgccgacgcc gcctgggaat gcgtcaagag cttcccggag ccggcctgcg tcatcgtcaa
     7561 gcacgccaat ccctgcggcg tcgccacggg acaaaacctg ctcgccgcgt atcagaaggc
     7621 tttcaagacc gatccggtgg ccgccttcgg cggcatcctc gctttcaacc gcgcgctcga
     7681 ccaggcagcc gcgcaagcgc tgggcaagca gttcgcagag gtcgtgattg cgccgcggat
     7741 tgaagctgac gcggctcacg aattgtcgaa gaagaccaat ctgcgcctgc tggaggcgcc
     7801 gctgtcgcac gatgtccaga ggcatgattt caagcgcgta ggcggcgggc tactcgtaca
     7861 gactaccgat tcgagggttt tgaaaaagga agatctgaag gttgtgacga aaaagcagcc
     7921 aagcgaagcg cagtggagcg atctgctgtt cgcctggcgc gtggcgaagt tcgtgaaatc
     7981 gaacgcgatc gtgttctgcc gcgacggcat gacgctcggc atcggcgccg gccagatgag
     8041 ccgcgtcgac agctcgcgcc tcgcggcaat gaaggcgcaa gcggcgaagc tgccgctcgc
     8101 cggcgccgca gtggcttcgg acgccttctt tccgttccgc gacgggcttg acgtagtgct
     8161 cgaggccggc gccacggcgg tgatccagcc cggcggcagc gcgcgagacg aggaagtcat
     8221 cgccgcggcc gacgcgcgcg cggtggccat ggtgttcacc ggggtgcggc acttcaagca
     8281 ctagatgcgc accttcagcc actgagatga agctgctcgt catcggctcg ggcgggcgcg
     8341 agcatgcgat cgcgtggaag ctcacgcaga gcccgcgcgt gcagaaggta tttgtcgcac
     8401 ctggcaacgc cggcaccgcc ctcgaaccgg gattggaaaa cgtcgccttg accgccattt
     8461 ccgacctgat ttctttttca aagcaaaaag aaatccagct taccgtggtc ggacccgagg
     8521 cgccgcttgc cgcgggcatc gtcgatgcat tccgcgatgc ggggctgcgc atcttcgggc
     8581 cgacgcgcgc cgcggcgcag ctcgaaacat cgaaggactt cgccaaggcg ttcatggtgc
     8641 gccatggcat cccgaccgcc cgctacgcca cgttcgctcg cgccggggag gcgaaagccc
     8701 acgtcgagaa gcacggcgcc ccgatcgtta tcaaggctga cggccttgcc gccggcaagg
     8761 gcgtggtggt cgcggcaacg cgcgaggaag cgcatgccgc gatcgacctg atgctcgaag
     8821 acaaaaggct cggcgccgcc ggcagccgcg tggtgatcga ggaatgcctg caaggcgagg
     8881 aagcgagctt catcgtgatg agcgatggcg tgaacgtgct ggcgctcgcc accagccagg
     8941 accacaagcg cctgtgcgac ggcgacgccg ggcccaacac cggaggcatg ggcgcctatt
     9001 cgccggcacc ggtggtgtcg cccgaggtgc actcgcgcgt gatgcgcgag atcattctgc
     9061 ccgcggtgca gggcatggcg aaggacggcc tgccttatgc cggctttctc tacgccggcc
     9121 tgatgatcga tcgctcgggc aatccccgga ctctcgagtt caactgccga ctgggcgacc
     9181 cggaggcgca accgatcatc ctgcggctga agtcggatct gttcgagctg ctcgagcatg
     9241 cgctcgccgg gacgcttgac cgcgtggagg cgaactggga ccggcgctcg gcgctcggcg
     9301 tggtgctcgc agcgcacggc tatcccgacg agccgcgcaa gggcgatcgc gtcgaaggct
     9361 tgcccccgcc gagcgaggat tgccgggtgt ttcacgccgc gacacggctc gaaggcaaga
     9421 gcgttctcac caacggcggg cgcgtgctgt gcgtcaccgc gctcggcgac tcggtgaaaa
     9481 tggcgcgcag ccgcgcctac gaggccgtgg agcacatcgg cttcgacggc atgcaatacc
     9541 gcaaggacat cggccaccgg gctctgaaga aacatccatg acgccgcggg cgcaggcggt
     9601 tcacgagtat ttcgccgaac tgcaggcgcg catcgtcgcc gcgctcgagg ctctggatgg
     9661 aacgtctttc ggaagcgatt cctggcagcg gcccgaaagc ggcggcggca tcacccgggt
     9721 cgtcgagcag ggccgcgtgt tcgagcgtgg cggcgtcaat ctctcgcgcg tgcaggggcg
     9781 cgagctgccg ccctcggcg
//
LOCUS       DASPXW010000186         3363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_809137, whole genome shotgun sequence.
ACCESSION   DASPXW010000186 DASPXW010000000
VERSION     DASPXW010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809137"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VEL04_04430"
     CDS             complement(<1..413)
                     /locus_tag="VEL04_04430"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HYT47642.1"
                     /translation="MTRLALGLMWLIHFLPLAVVAAIGNALGALLYWLIPERRRVTRI
                     NLAKCFPQMPEAERERLARASFRAFARSFIERGILWWAPTARVARLVRIEGIEHLQAL
                     GHNRVVLFGPHFVGLDAVVRLNIDLPMVSTYSRQK"
     gene            complement(410..1276)
                     /locus_tag="VEL04_04435"
     CDS             complement(410..1276)
                     /locus_tag="VEL04_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HYT47643.1"
                     /translation="MHRLLRFVLHGASKLPLSALHALGSVLGWMMYGISPTYRRHLRQ
                     HLAQAGYADAATRRAAIATAGQMLAELPALWFRPYHEVVQLVKEVEGAEVAYAAHAQG
                     KALLFLAPHMGCFEITAQYAAQRVPLTVLYRRPKLAWLEPAMLEGRSRPNLRLVPANF
                     SGVRRLFKALRRGEAVGFLPDQVPSEGEGEWADFFGRPAYTMTLAARLAESGNVACFL
                     AYARRLPRAAGYAFVVRPFPEILPDETATRRLNRAIEELVRECPGQYLWGYNRYKTPA
                     GARPPERRESTA"
     gene            1325..2482
                     /gene="metK"
                     /locus_tag="VEL04_04440"
     CDS             1325..2482
                     /gene="metK"
                     /locus_tag="VEL04_04440"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006024140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HYT47644.1"
                     /translation="MNEFLFTSESVSEGHPDKVADQISDAVLDAILAQDPRSRVAAET
                     LVKDNLVVLAGEITTRAQVDYDRVARATIRRIGYNDPALGFDAATCTVQIAYGQQSPD
                     IARGVDEGKGLDLEQGAGDQGLMFGYACDETPSLMPLPIYLSHRLVERQSEVRRDGRL
                     PWLRPDAKSQVTIKYLNGKPDSIETVVLSTQHAPGLTHQRIEEDVIEQIIKPVLPKSM
                     VKGKISYLVNPTGSFEIGGPKGDCGVTGRKIIVDTYGGSAPHGGGAFSGKDPSKVDRS
                     AAYAARYVAKNIVAAGLASKCLVQVSYAIGVAQPTSVMVTTQGSSKLSDEKLAQLVRA
                     NFDLRPKGIIQMLDLLRPIYEKTAAYGHFGREEPEFTWEATDKALALKKAA"
     gene            2496..>3363
                     /locus_tag="VEL04_04445"
     CDS             2496..>3363
                     /locus_tag="VEL04_04445"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47645.1"
                     /translation="MSHRNLEELLRASGNPVAMLRNSQIGAYVYPVVPYEFSNWRDEQ
                     IAWQKSAVLFDQSHHMVEQYVEGPDAMKLFSHLGGNSFASFPVNRAKQLAPCSYDGYV
                     IGDGILFHLGENRLVFVGRAPAASWIQFHAETGGFKVKTEKDDRSPGNAKGKAVVRRN
                     YRFQIQGPNAARVIEKLHGGPMPEMKFFHMASINIAGRKVRALRHGMAGAPGLEIWGP
                     YEEREEIRAAIVAAGKDFGLKEVGARAYATNTLESGWIPSPLPAVYTGEKLKAYRQWL
                     PADYYEAKASIGG"
BASE COUNT          632 a         1113 c         1116 g          502 t
ORIGIN      
        1 tccttctgcc gcgaataggt cgagaccatc ggcaagtcga tgttcagccg caccaccgcg
       61 tccaggccga cgaaatgcgg accgaacagg accacgcggt tgtggccgag cgcctgcagg
      121 tgctcgatcc cttcgatgcg caccagccgc gcgacgcgag cggtcggcgc ccaccacagg
      181 atgccgcgct cgatgaagct gcgcgcgaag gcgcggaaac tcgcgcgcgc cagcctttcc
      241 ctctccgctt ccggcatttg cgggaagcat ttcgcgagat tgatgcgcgt cacgcgcctg
      301 cgctcgggaa tcagccagta gagcagcgcg ccgagagcgt tgccgatcgc cgcgaccacc
      361 gcgagcggca ggaagtggat cagccacatc aggcccagag cgagccgggt cacgccgtgc
      421 tttccctgcg ctcgggcggc cgggcgccgg ccggggtctt gtagcggttg tagccccaga
      481 ggtactggcc cgggcactcg cgcaccagct cttcgatcgc gcggttgagc cgccgtgttg
      541 cggtttcatc gggaagaatt tctggaaaag gccgcaccac aaacgcgtag ccggcggcgc
      601 gcggcagccg tcttgcgtag gcgagaaaac aggcgacatt cccgctttcg gcgagcctcg
      661 cggcgagcgt catcgtatac gccgggcggc cgaagaaatc cgcccattcg ccctcgcctt
      721 cgctcggcac ctggtccgga agaaagccga ccgcctcgcc gcgccgcagt gccttgaaga
      781 gccggcgtac gccggagaaa ttggcgggaa ccagccgcag attgggccgg ctgcgccctt
      841 cgagcatcgc cggctcgagc caggcgagct tcggccgccg gtacaacacg gtgagcggca
      901 cgcgctgcgc ggcgtactgc gcggtgatct cgaagcatcc catgtggggg gcgagaaaca
      961 gcagcgcctt cccctgcgcg tgcgcggcgt acgccacctc ggcgccttcg acctccttca
     1021 ccagctgcac cacctcgtgg tacggacgga accacagcgc gggcagctcg gcgagcatct
     1081 gtcccgctgt cgcgatcgcc gcgcgacgcg tggcggcatc ggcatagccg gcctgcgcga
     1141 gatgctggcg cagatgcctg cgataagtgg gcgagatccc atacatcatc cagccgagga
     1201 cgcttcccaa cgcatgcagc gccgaaagcg gcaatttcga tgcgccatgc aggacaaacc
     1261 tgaggagtcg atgcaagatt tggtatcctt gcggccttcc ctagactcaa agcaagcgcg
     1321 ggcaatgaac gaatttcttt ttacttctga atcggtatcc gaaggccacc cggacaaggt
     1381 cgctgaccag atctcggacg cggtgctcga cgcgattcta gcccaggacc cccgctcccg
     1441 cgtcgcggcg gagacattgg tgaaggacaa cctggtggtg ctggcgggcg agatcaccac
     1501 gcgcgcgcag gtcgactacg accgcgtcgc gcgcgcgacc attcggcgca tcggctacaa
     1561 cgatccggcg ctgggcttcg atgcggcgac ctgcaccgtg cagatcgcct acggccagca
     1621 atcgcccgac atcgcgcggg gcgtggacga gggcaagggc ctggacctgg agcagggggc
     1681 gggcgaccag gggctcatgt tcggctacgc ctgcgacgag accccgagcc tgatgccgct
     1741 gccgatctat ctctcgcacc ggctggtcga gcgccaatcc gaagtgcgcc gcgacgggcg
     1801 cctgccctgg ctgcggccgg atgccaaatc gcaagtgaca ataaagtatt tgaatgggaa
     1861 accggattcc atcgagacgg tggtgctctc gacgcagcac gcgccaggcc tcacacacca
     1921 gcggatcgag gaagacgtga tcgagcagat catcaagcca gtactgccga agagcatggt
     1981 gaagggcaag atcagctacc tcgtcaaccc gaccggcagc ttcgagatcg gcgggccgaa
     2041 gggcgactgc ggggtcaccg gccgcaagat catcgtcgac acctacggcg gctccgcgcc
     2101 gcacggcggc ggcgcgttct cgggcaagga tccgtccaag gtggaccgct cggccgccta
     2161 tgccgcgcgc tacgtggcga agaacatcgt cgccgccggc ctcgcctcga agtgcctggt
     2221 gcaggtgtcc tacgccatcg gcgtggccca gcccaccagc gtgatggtca ccacccaggg
     2281 cagcagcaag ctgtccgacg agaagctggc gcagctggtg cgcgcgaact tcgacctgcg
     2341 gcccaaaggc atcatccaga tgctcgacct gctgcgcccg atctacgaga agaccgccgc
     2401 gtacggccac ttcggccgcg aggagcccga gttcacctgg gaagcgaccg acaaggcgct
     2461 ggcgctgaaa aaagcagcct gaggaggaga accccatgag tcaccgcaat ctcgaggagc
     2521 tgctgagagc ctcaggcaat ccggtggcga tgctgcgcaa ctcgcagatc ggcgcctacg
     2581 tctatccggt ggtgccgtac gaattctcca actggcgcga cgagcagata gcctggcaga
     2641 agagcgcggt gctcttcgac cagtcgcacc acatggtcga gcagtacgtc gagggtcccg
     2701 acgcgatgaa gctcttttcc catctcggcg gtaacagctt cgccagcttt ccggtcaacc
     2761 gcgccaagca gctcgcgccg tgcagctacg acggctatgt catcggcgac ggcattctct
     2821 tccacctggg ggagaaccgg ctggtgttcg tcgggcgcgc gccggcggcg agctggatcc
     2881 agttccatgc cgagaccggc ggcttcaagg tgaagaccga gaaggacgac cgctcgcccg
     2941 gcaatgccaa gggcaaggcg gtggtgcgca ggaactaccg cttccagatc caggggccaa
     3001 acgcggcgcg agtgatcgaa aagctgcacg gcgggccgat gccggagatg aaattcttcc
     3061 acatggcctc gatcaacatc gccggacgga aggtgcgcgc cctgcgccac ggcatggccg
     3121 gcgcgcccgg gctcgagatc tggggaccgt atgaggagcg cgaggagatc cgcgccgcga
     3181 tcgtcgccgc cggcaaggac ttcggcctga aggaagtcgg cgccagggcc tatgccacca
     3241 atacgctcga gtcgggctgg atcccgtcgc cgctcccggc cgtctacact ggcgagaagc
     3301 tgaaggcgta ccggcagtgg ctgccggccg attactatga agcgaaggcg tcgattggcg
     3361 gca
//
LOCUS       DASPXW010000187         2174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_934870, whole genome shotgun sequence.
ACCESSION   DASPXW010000187 DASPXW010000000
VERSION     DASPXW010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934870"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VEL04_04450"
     CDS             complement(<1..436)
                     /locus_tag="VEL04_04450"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYT47646.1"
                     /translation="MEERVNFVSAGLRLAGILHLPDDLKTGARRAAFLVLHGFGSNKD
                     SSSMIAVARTLVSLGYVALRFDMRGCGESEGVRGRVICLEQVEDTRNAVSYLASRGEI
                     DAKRIGVLGHSFGAAVAVYAAGVEPRLAACVSSGGWGDGETKF"
     gene            460..1401
                     /locus_tag="VEL04_04455"
     CDS             460..1401
                     /locus_tag="VEL04_04455"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYT47647.1"
                     /translation="MKFILLFLYCGLALAQTYPSRPIKLVTPFPPGGSADVIARLTAH
                     KLGEALSQPVVVDNRAGAGGVVGNDYAAKQPPDGYTLLLITGAYPVQAAMLKSLPFDP
                     LADIAMVSLLTTYPFVISVRPDSPLKSVGDLIAQAKAHPGKLNYPSSGIGTVHHLSGE
                     LFNAMASTEIFHVPFRGGAGPLTEVLAARVDILLEAMTLSIGQIQSGKLRALAVTSRS
                     RWKALPDVPAVHETLPGYEVISFIGLGATGGTPAPIIERVNGEVRKMLDSADGSKRFI
                     DLGGEPRASSPEEMKSFIEREIAKWREVIAARKIERQ"
     gene            1401..>2174
                     /locus_tag="VEL04_04460"
     CDS             1401..>2174
                     /locus_tag="VEL04_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HYT47648.1"
                     /translation="MLVDWDAPIPMDDGIVLCADVFRPLAEGRYPVILSYGPYAKGLA
                     FQEGYKSNWARLVKAAPEVLEGSSNKYQCWELVDPEKWVPDGYACVRVDSRGAGRSPG
                     YLDIWSAREARDLYHCVEWAGTQPWGSGKVGLNGISYFATNQWQAGALRPPHLAALCI
                     WEGFADYYRELARHGGILSGFIESWYERQILRVQHGVGENGPRSRVTGEPVAGPETLP
                     AETLARNHADPAREALRRRLIDDYYRSRMVEFERIEAPLL"
BASE COUNT          366 a          757 c          717 g          334 t
ORIGIN      
        1 ggaatttggt ctcgccatcg ccccagccgc ccgaagagac gcaggccgcg agccgaggct
       61 cgacgcccgc cgcgtacacc gcaaccgcgg cgccgaagct gtggcctagg acgcctatgc
      121 gctttgcatc tatctctcca cgggaggcaa ggtatgaaac tgcatttctc gtgtcctcga
      181 cctgctccag gcagatgacg cggccgcgga cgccttcgct ctcgccgcag ccgcgcatgt
      241 cgaaacgcag cgccacgtag cccaggctga cgagggtacg cgccaccgcg atcatcgagc
      301 tcgagtcctt gttgctgccg aagccatgca gcacgagaaa ggcggcgcgg cgtgcgccgg
      361 tcttgaggtc gtccggcagg tgcaggattc cggcgagccg caggccggcg gaaacgaaat
      421 tgacgcgctc ttccatgacg cgagcataaa ctagcggcga tgaaatttat cctgctgttt
      481 ctttattgtg gactggcgct cgcgcagact tatccctcga ggccgatcaa gctcgtgacg
      541 ccgtttccgc ccggcggctc ggccgacgtc attgcgcgcc ttacagcgca caagctcggc
      601 gaggcgctca gccagccggt cgtggtcgac aaccgcgccg gcgccggcgg cgtggtcggc
      661 aacgactacg cggcgaagca gccgcccgac ggctacacgc tgctcctcat caccggcgcc
      721 tacccggtgc aggctgcaat gctgaaatcg ctgcccttcg acccgctcgc ggacatcgcg
      781 atggtctccc tgctgaccac atatccgttc gtcatcagcg tgcggcccga ttcgccgctc
      841 aagagcgtcg gcgacctgat cgcgcaggcg aaggcgcacc ccggcaagct caactacccc
      901 tcgtcaggca tcggcaccgt gcatcacctc tccggcgagc tgttcaatgc catggcaagc
      961 accgagatct tccacgtgcc gttccgcggc ggcgccgggc cgctgaccga ggtgctcgcc
     1021 gcccgggtcg acatcctgct cgaggcgatg acgctctcga tcggccagat ccagtccgga
     1081 aagctgcggg cgctcgcggt gacttcgcgc agccgctgga aagcgctgcc cgacgttccc
     1141 gcggtccacg agaccctgcc cggctatgaa gtgatctcgt tcatcggcct cggcgccacc
     1201 ggcggcacgc cggcgccgat catcgaacgc gtcaacggcg aagtgaggaa gatgctcgac
     1261 agcgccgacg gcagcaagcg cttcatcgac ctcggcggcg agccgcgtgc ctcgagcccg
     1321 gaggagatga agagcttcat cgagcgcgag atcgcgaaat ggcgcgaggt gatcgcggcc
     1381 cgcaaaatag agcgccagtg atgctggtcg actgggacgc gccgatcccg atggacgacg
     1441 gcatcgtcct gtgcgccgac gtgtttcggc cgctcgccga aggccgctat ccagtcatcc
     1501 tcagctacgg cccgtacgcg aagggcctcg ctttccagga gggctacaag tcgaactggg
     1561 cgcgcctcgt caaggccgcg cccgaggtgc tcgagggatc gagcaacaag taccagtgct
     1621 gggagctggt cgatcccgag aagtgggttc cggacggcta cgcgtgcgtc cgcgtcgatt
     1681 cgcgcggcgc cggccgctcg cccggctacc tcgacatctg gtcggcgcgc gaggcgcgcg
     1741 atctctacca ctgcgtcgag tgggccggca cgcagccttg ggggagcggc aaggtgggcc
     1801 tcaacggcat ctcctacttc gccaccaacc agtggcaggc cggcgcgctg cggccgccgc
     1861 atctcgcggc gctctgcatc tgggaaggct tcgccgacta ttaccgcgag ctcgcgcgcc
     1921 acggcgggat cctcagcggc ttcatcgaga gctggtacga gcgccagatc ctgcgcgtgc
     1981 agcacggcgt cggcgagaac ggcccgcgca gccgggtgac cggcgagccg gtggcggggc
     2041 cggaaactct gccggcggag acgctcgcga gaaaccacgc cgacccggcg cgagaggccc
     2101 tgcggcggcg gctcatcgac gattactacc gcagccgcat ggtcgagttc gaacgcatcg
     2161 aagcgccgct gctc
//
LOCUS       DASPXW010000188         5539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1190591, whole genome shotgun sequence.
ACCESSION   DASPXW010000188 DASPXW010000000
VERSION     DASPXW010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5539
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190591"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="VEL04_04465"
     CDS             <1..709
                     /locus_tag="VEL04_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HYT47649.1"
                     /translation="ELIQLGKLDRVIVGGAEEVRWTSALVFDAMGALSRGFNDTPEWA
                     SRPYDKRRDGFVLAGGAGVLVLEELESALARGARVRAELAGYGTSSDGSNMVTPRAEG
                     IARAMRMALAEANAEVDYINTHATSTPLGDRTELEAIREVFGDRPPLISSTKGLTGHA
                     VGASGAHEAIYCLLMMERGFIAACANLFEPDPATHGLPLVTAARDASLETVMTNSLGF
                     GGTNATLVLRRWQGKPG"
     gene            688..1302
                     /locus_tag="VEL04_04470"
     CDS             688..1302
                     /locus_tag="VEL04_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47650.1"
                     /translation="MARKAWIAAARIADLRGHALVEARRRLGPRDRRRYAAFGTQKRR
                     REFLAGRWLLEQSARAWRPDARLRFDSSPTGGISVNRRIGASVSHARGWVACALVESG
                     RPGIDIEPMVERDFTALDKWIADDEGDAGPFVDGGADARKTFYGRWTRYEARLKALGE
                     AKRRASHVEVTYYLEGGVALSVCLPANAAARVPRKPVILMVNSH"
     gene            1344..3233
                     /locus_tag="VEL04_04475"
     CDS             1344..3233
                     /locus_tag="VEL04_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005663967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HYT47651.1"
                     /translation="MGTSSQHSPQSSTAALTLLALGVVYGDIGTSPLYAVKETFNPEH
                     GIALNTENILGGLSAIFWALMIVVSLKYVMLVLRANNRGEGGIMALLALATASIRKHP
                     QWSAPLLAIGVFGASLFYGDAVLTPAISVLSAVEGLQVGTSAFKPYVVPLATGILIAL
                     FLIQKHGTALVGMLFGPVCALWFFSIGASGIWNIGKNPQILEALNPLRALHFVTGHGF
                     ASFVVLGSVLLAITGAEALYADMGHFGKSAIRIAWFGLVAPALVLNYFGQGALLIADP
                     KALENPFYLAYPSWALYPMVALATAATVIASQATISGAYSMTQQAIQLGYLPRMTIHH
                     TSARTIGQIYAPVVNWILLFAVAAAVIGFGSSTNLASAYGVAVMGTMLVTTFLTFFVI
                     RYGWGYPLWLCLLATGFFTLIDATFFAAAMHKVLEGGWFPLLLGAGVFMIMTTWRRGR
                     EILFARLSAAAIPLHAFLESLFRAPPQRVPGTAVFLTAIPDITPNSLLHSLKHYKVLH
                     ERVVFLTVEFLDVPSVPQEDRAAVEKLNQDCYRLSARYGFMDRPDAIAALEAARAKGL
                     DLDLMETSFFLSREKIIPAAGTGGMMLWRERLFAAMARNSGNISDYFNIPPNRVVELG
                     TRIEF"
     gene            complement(3236..3985)
                     /locus_tag="VEL04_04480"
     CDS             complement(3236..3985)
                     /locus_tag="VEL04_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYT47652.1"
                     /translation="MTQTSSRSRSDAELLEANRRFYDPLWRGARLVEPERFNTWPLVC
                     SLISPSRPRLEVAPGLRPRLPIEGTHFVDISAPAIAKLRRRGANAMLGLVSALPFPDA
                     MFDLVCAFDIVEHVDDEDGALSELSRVAAPGAAFLLSVPLHPSHWTAFDDFVGHRRRY
                     EPGRLLAKLAQHGFRVEQSAVYGMRPKSSRLVDLAMWALTHQRGKALWWYNHVFMPLG
                     VRFQKKLALAPGMIDAENVDEVLLVCRKQGA"
     gene            complement(4007..4083)
                     /locus_tag="VEL04_04485"
     tRNA            complement(4007..4083)
                     /locus_tag="VEL04_04485"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4047..4049),aa:Arg,seq:ccg)
     gene            4127..4648
                     /locus_tag="VEL04_04490"
     CDS             4127..4648
                     /locus_tag="VEL04_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HYT47653.1"
                     /translation="MSEHGTATSFIKTPQQLIVVILLSFLVPIVGIVLLVQLIMSPQS
                     ADPGAMTPEAVAARIQPVGRLEFGAARGAAGARSGEEIVKSVCAACHQAGVANAPKIG
                     DKAAWAPHIKEGLKDMLATALKGKGAMPPRGGDASLSDDEVARAVVFMANQAGASFKA
                     PAAPKQKPAAAGK"
     gene            4656..>5539
                     /gene="gshB"
                     /locus_tag="VEL04_04495"
     CDS             4656..>5539
                     /gene="gshB"
                     /locus_tag="VEL04_04495"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004198015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HYT47654.1"
                     /translation="MQLLFVVDPPDTLKAYKDTTVSMMRAAQARGHGVWVCEVKHLEW
                     KGKTAAAATRISLTDNDEDWYRAHQTEMRPLAQFAAVLMRKDPPFDLEYVVSTWLLSA
                     AVREGAKVFNAPDAIRDHNEKLAIAEFARFAAPTLVTREPGRVQAFIDAERDVVLKRL
                     DVMGGENIFRVRADDPNRNVIVETMSQGGSRSVMVQRYLPQITEGDKRVLLIDGRAVP
                     HCLARIPKPGESRGNLAAGARGVAQPLSARQREIAEALGPKLAARGLFLVGLDVIGDS
                     LTEINVTSPTCFREIQDQT"
BASE COUNT          901 a         1889 c         1824 g          925 t
ORIGIN      
        1 ggagctgatc cagctcggca agctcgaccg cgtaatcgtc ggcggagcag aggaagtacg
       61 ctggacgagc gcgctggtgt tcgacgccat gggcgcgttg tccaggggat tcaacgacac
      121 gccggagtgg gcctcaaggc cttacgacaa gcgccgcgac ggcttcgtgc tggcgggcgg
      181 cgcaggagta ctcgtgctcg aggagctcga gagcgccctc gcgcgcggcg cgcgggtgcg
      241 cgcggagctc gccggctacg gcacttcgtc cgacggctcg aatatggtga ctcccagggc
      301 cgagggcatt gcgagagcga tgcgcatggc gctcgcagag gccaacgccg aagtcgacta
      361 tataaatacc cacgccacct cgacgccact gggagaccgc actgagctcg aggcgatacg
      421 cgaagtgttc ggagataggc cgccgctcat ctcctcgacc aaaggcctga ccggacatgc
      481 cgtcggcgcc tccggagcgc acgaggcgat ctattgcctg ctgatgatgg agcgcggctt
      541 catcgcggcc tgcgccaatc tcttcgagcc cgatccggcg acgcacggcc tgccgctcgt
      601 cacggccgca cgcgacgcct cgctcgagac cgtcatgacc aacagcctgg gctttggcgg
      661 caccaacgcc accctggtat tgcgccggtg gcaaggaaag cctggatagc ggcggcgcgc
      721 atcgccgacc tccgcggcca tgcgctcgtc gaagcacgca ggcgcctcgg gccgagagac
      781 cgccgccgtt atgcggcgtt cggcacccag aagcgccggc gcgaatttct cgccgggcgc
      841 tggctgctcg agcagtccgc gcgcgcctgg cgccctgatg cgcggttgcg tttcgattct
      901 tcgcccaccg gcgggatcag tgtgaaccgc cgcattggcg cgagcgtctc ccacgcccgc
      961 ggctgggtcg cgtgcgcgct ggtcgagtca ggccgccctg gcatcgatat cgagccaatg
     1021 gtcgagcgcg acttcaccgc gctggacaag tggatcgcgg acgacgaggg cgatgccggg
     1081 ccgttcgtgg acggaggagc cgatgcccga aagaccttct acgggcgctg gacgcgttac
     1141 gaagcgcgct tgaaagcgct gggtgaagcg aagaggcgcg cgtcccacgt cgaggtcacc
     1201 tattacctcg agggcggcgt cgcgctttca gtctgcctgc cagcgaacgc tgccgcccgt
     1261 gtacctcgta aacctgtgat tctcatggta aacagccact agtttgcctt ttgtgcagtg
     1321 cgggagtagc gtgctcgccc accgtgggca cgagtagcca gcactcaccg cagtcgtcca
     1381 ccgcagcgct cacactgctc gcgctcggag tcgtttacgg cgacatcggg accagcccgc
     1441 tgtacgcggt gaaagagacg ttcaatcccg agcacggcat tgcgctcaac accgaaaaca
     1501 ttctcggcgg gctgtcggcg attttctggg cgctgatgat cgtggtgtcg ctgaagtacg
     1561 tgatgctcgt gctgcgcgcg aacaaccgcg gcgagggcgg catcatggcg ctgctcgcgc
     1621 ttgccaccgc ctcgatccgc aagcatccgc aatggagcgc gccgctgctg gcgatcggcg
     1681 tgttcggcgc ctcgctattt tacggcgacg ctgttctcac gccggccatt tccgtgcttt
     1741 cagcggtgga agggttgcag gtcggaactt cggcgttcaa gccttatgtc gtgccgcttg
     1801 ccaccggcat cctgatcgcg ctgttcctca tccagaagca cggcacagcg ctggtgggca
     1861 tgctgttcgg cccggtgtgc gcgctctggt ttttctcgat cggcgcctcg ggcatctgga
     1921 acatcggaaa gaatccgcag atcctggagg cgctgaatcc actgcgcgcg ctgcacttcg
     1981 tcaccgggca cggcttcgcc tcgttcgtgg tgctcggctc ggtgctgctc gccattacgg
     2041 gcgccgaggc gctctacgcc gacatggggc atttcggcaa gagcgccatc cgcatcgcgt
     2101 ggttcggcct ggtcgcgccc gcgctggtgc tgaactattt cggacagggc gcgctgctga
     2161 tcgccgaccc gaaggcgctc gagaatccgt tctacctggc ttacccgtcc tgggcgctct
     2221 acccgatggt ggccctggcg actgcggcaa ccgtgatcgc ttcacaggcg accatttccg
     2281 gggcctactc catgacgcag caggcgatcc agctcggcta cctgccgcgc atgacgatcc
     2341 accatacgtc ggcccggacc atcggacaga tctacgcccc ggtcgtgaac tggattctcc
     2401 tgttcgcggt cgcagcagca gtaatcgggt tcggctcctc gaccaacctc gcctccgcct
     2461 acggcgtcgc cgtgatgggc acgatgctcg tcaccacctt cctcacgttc ttcgtgatcc
     2521 gctatggctg gggctatccg ctctggctgt gcctgctcgc caccggcttt ttcacgctga
     2581 tcgacgccac cttcttcgcc gccgcgatgc acaaggtgct ggaaggcggc tggttcccgc
     2641 tgctgctcgg tgcaggcgtg ttcatgatca tgacgacctg gcggcgcggc cgcgagattc
     2701 tgttcgcccg tctgagcgcc gcggcgattc cgctgcacgc cttcctcgag agcctgttcc
     2761 gcgccccgcc gcagcgcgtg cccggcaccg cggtattcct caccgcaatt cccgatatca
     2821 cgccgaactc gctgctccac agcctcaagc actacaaggt gctgcacgag cgcgtggtgt
     2881 ttctcaccgt ggaattcctc gacgtgcctt cggtccccca ggaagaccgt gccgcagtgg
     2941 aaaagctcaa tcaggactgt taccgccttt cggcgcgcta cggcttcatg gaccggcccg
     3001 acgctatcgc ggcgctcgag gcggcgcgcg ccaaggggct cgatctcgat ctgatggaga
     3061 cctcgttttt tctatcgcgc gagaagatca ttccggctgc gggcacggga ggcatgatgc
     3121 tctggcgcga gcggctgttc gccgccatgg cgcgcaactc cggcaacatc tccgactact
     3181 tcaacatccc gcccaatcgc gtggtcgagc tcggcacgcg tatcgagttc tgacgctacg
     3241 cgccttgctt gcggcagacg agcaaaacct cgtccacatt ttcggcgtcg atcatcccgg
     3301 gggcaagggc gagcttcttc tggaagcgca cgccgagcgg catgaagaca tggttgtacc
     3361 accacagcgc cttcccgcgc tggtgggtga gagcccacat ggcgaggtcg accaggcgcg
     3421 aggatttcgg ccgcatgccg taaacggcgc tttgctccac ccggaagccg tgctgcgcga
     3481 gcttggcgag gagccgcccg ggctcgtagc ggcggcggtg cccgacgaag tcgtcgaacg
     3541 ccgtccagtg cgacggatgc agcggcaccg agagcaggaa cgccgcgccg ggcgcggcca
     3601 cgcgcgacag ctcggacagc gccccatcct cgtcgtcgac atgctcgacg atgtcgaagg
     3661 cgcacaccag atcgaacatg gcgtccggaa acggcagcgc gctcaccagg cccaacatcg
     3721 cgttggcgcc gcgcctgcgc agcttggcaa tcgccggcgc gctgatgtcg acgaagtgcg
     3781 tcccctcgat cggcagcctc ggcctcagcc ccggagcgac ttcgagccgc ggtcgcgacg
     3841 gcgagatcag ggagcacacc agcggccagg tgttgaagcg ctcgggctcg accagccgcg
     3901 cacccctcca cagcgggtcg tagaagcgcc ggttcgcttc gaggagctcg gcgtcgctgc
     3961 gggagcgtga cgacgtttga gtcaccgcgc agtcacgcta tgtggctggc gcgcccggcg
     4021 ggattcgaac ccacgaccct tggcttcgga ggccaatact ctgatccgct gagctacggg
     4081 cgcccacgcg ctagaatcat acctttatcc caaaacaaac accccagtga gcgagcacgg
     4141 caccgctacg tctttcatca agaccccgca gcagctgatc gtcgtgatcc tgctttcgtt
     4201 cctggtgccg atcgtcggca tcgtcctgct ggtgcagctc atcatgagcc cccagagcgc
     4261 cgacccgggc gcgatgacgc cggaagccgt ggcggcgcgc atccagcccg tcgggcggct
     4321 cgagttcggc gcagcgcgcg gcgccgcggg cgcgcgcagc ggcgaggaga tcgtcaagtc
     4381 ggtgtgcgca gcctgccatc aggccggcgt ggcgaacgcg cccaagatcg gcgacaaggc
     4441 ggcgtgggca ccgcacatca aggaagggct gaaggacatg ctcgccactg cgctcaaggg
     4501 caagggcgcc atgccgccgc gcggcggcga tgcatcgctc tcggacgacg aggtggcgcg
     4561 cgccgtggta ttcatggcga accaggctgg cgcgagcttc aaggcgcccg ccgcgccgaa
     4621 gcagaagccg gcggcggccg gcaaatagcc tggccctgca gctgcttttc gtcgtcgatc
     4681 cgcccgatac gctcaaggcg tacaaggaca ccacggtcag catgatgcgc gccgcgcagg
     4741 cgcgcggcca cggcgtgtgg gtgtgcgaag tgaagcatct cgagtggaag ggcaagaccg
     4801 ccgccgcggc cacccggatt tcgctcaccg acaacgacga ggactggtac cgcgcgcacc
     4861 agaccgagat gcggccgctc gcgcagtttg ccgcggtgct gatgcgcaag gatccgccct
     4921 tcgacctcga gtacgtggtg agcacctggc tgctgtcggc ggcagtgcgc gagggcgcca
     4981 aggtgttcaa cgctcccgat gcgatccgcg accacaacga gaagctcgcc atcgccgagt
     5041 ttgcgcgctt cgccgcaccg acgctggtca cgcgcgagcc cggccgtgtc caggcgttca
     5101 tcgacgccga gcgcgacgtg gtgctgaagc ggctcgacgt catgggcggg gagaacatct
     5161 tccgcgtgcg cgccgacgat ccgaaccgca acgtcatcgt cgagaccatg tcgcagggcg
     5221 gctcgcgcag cgtcatggtg cagcgctacc tgccgcagat cacggagggc gacaagcgcg
     5281 ttctcctgat cgacggcagg gcggtgccgc actgcctcgc gcgcattccg aagcccggcg
     5341 agtcgcgcgg caacctcgcc gccggcgcgc gcggcgttgc ccagccgctc agcgcgcgcc
     5401 agcgcgagat cgccgaggcg ctcggaccga agctcgcggc gcggggactg ttcctggtcg
     5461 ggctcgacgt catcggcgac tcgctcaccg agatcaacgt caccagcccg acctgcttcc
     5521 gcgagatcca ggaccagac
//
LOCUS       DASPXW010000189         3072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_180228, whole genome shotgun sequence.
ACCESSION   DASPXW010000189 DASPXW010000000
VERSION     DASPXW010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180228"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..166)
                     /locus_tag="VEL04_04500"
     CDS             complement(<1..166)
                     /locus_tag="VEL04_04500"
                     /inference="COORDINATES: protein motif:HMM:NF013538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HYT47655.1"
                     /translation="MKRLIIATRRSRLALWQAGHIKQRLEGVHRGLTVELLPMSTRGD
                     ELADLRLDEAG"
     gene            complement(163..924)
                     /locus_tag="VEL04_04505"
     CDS             complement(163..924)
                     /locus_tag="VEL04_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HYT47656.1"
                     /translation="MSALRVFIADDEEPARERLKELLNDIAAELPTSIVGEARHGLET
                     IELVPTSRAQVLLLDIQMPGMGGLEVARHLASLENAPAVVFVTAHERHAVEAFELNAL
                     DYLLKPVRAARLAAALRKAATAAAPGREQLARAASDAREYLSIAERHRIVLVPVRDII
                     FLMAEQKYVTLRTRERQHLIEEPLIALEREFAAQFVRVHRNCLVARAAIRGFERASGS
                     DEEPHWLVVLDGIDERLPVSRRQWPALRELVADKP"
     gene            complement(921..1931)
                     /locus_tag="VEL04_04510"
     CDS             complement(921..1931)
                     /locus_tag="VEL04_04510"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HYT47657.1"
                     /translation="MVSIRQNAYPDVLPDFRNLGVIARVLVAVNALALAGAVFAAPSF
                     RDALHRFVEAAAFVEPLLFLELLALFALSPWIARLPYWAGCAAVVALVTALAAAYQLA
                     VFGVPQGAALLARTIVLCAALAAGLLGYFRLLVKAFSPALAEARLQTLQARIRPHFLF
                     NSLNAVLALIRRDPRRAERSLEDLADLFRTLMSDARQFVRLSDEIALLERYAELEQLR
                     LGERLRITWELDAAPSDALLPPLVLQPLLENAVYHGVEPDTAAGEVLVRIERRGDRVI
                     ARIENPYIEAEQQRAGNRMALENIRERLALFFDAEARIETSLHGNRYRVEIEMPYRVA
                     QA"
     gene            1967..>3072
                     /gene="argH"
                     /locus_tag="VEL04_04515"
     CDS             1967..>3072
                     /gene="argH"
                     /locus_tag="VEL04_04515"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013211808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HYT47658.1"
                     /translation="MARKPQPWSGRFKDAVDERVKRFTASVGFDRRLAPYDLQGSLAH
                     ARMLAACGILPRRDLKAIERGLAQIRREIESGRFAWSIDAEDVHLNIERRLTALVGEP
                     GKRLHTARSRNDQVATDLRLWLRAEIDAIRKLVAALERSLLAQAARHAALLMPGFTHL
                     QVAQPITFGHHLLAYVEMLERDRERLIQVRSRVNRLPLGAAALAGTSFRIDREQVARE
                     LGFEGVCENSLDAVSDRDFAIEFAACAALVMVHLSRLAEELVLWASPRFGFVRLPERY
                     CTGSSIMPQKKNPDVPELVRGKSGRVFGDLVALLTLMKGQPLAYNKDNQEDKEPLFDA
                     AATVKSSLGVFAGLIADLVPVPRAMRAAALEGHAT"
BASE COUNT          491 a         1075 c         1053 g          453 t
ORIGIN      
        1 ctccggcctc gtcgagccgc aggtcggcga gctcgtcgcc gcgcgtcgac atcggcagca
       61 actcgaccgt gaggccgcga tgcacgcctt cgaggcgctg cttgatgtgc ccggcctgcc
      121 agagcgcgag gcggctgcgc cgggtggcga tgatgagccg cttcatggtt tgtcggccac
      181 cagctcgcgc agcgccggcc actggcgccg gctgaccggc aggcgctcgt cgatcccgtc
      241 cagcacgacc aaccagtgcg gctcctcgtc ggagccgctg gctcgctcga agccgcggat
      301 ggccgcgcgc gccaccaggc agttgcggtg gacgcgcacg aactgggcgg cgaattcgcg
      361 ctccagggca atcagaggct cctcgatcag gtgctggcgc tcgcgcgtgc gcagcgtgac
      421 gtacttctgc tcggccatga ggaagataat gtcgcgcacc ggcaccagga cgatgcggtg
      481 gcgctcggcg atcgacaggt attcgcgcgc gtcgctcgcg gcgcgcgcga gctgctcgcg
      541 ccccggggcg gccgccgtag cggcctttct cagcgccgct gcgagccgcg ccgcgcgcac
      601 cggcttgagc aggtagtcga gcgcattcag ctcgaacgcc tcgacggcgt gccgctcgtg
      661 cgcggtgacg aacaccactg cgggcgcgtt ttccagggaa gcgagatggc gcgccacctc
      721 gagccccccc atgcccggca tctggatgtc gagcaacagc acctgcgcgc ggctcgtcgg
      781 cacgagctcg atggtttcca gcccgtggcg tgcctcgccg acaatgctgg tcggcagctc
      841 ggcggcaatg tcgttgagca actccttcag gcgctcgcgc gccggctctt cgtcgtcggc
      901 gatgaacacg cgcagcgcgc tcatgcctgc gccacgcggt acggcatctc gatctccacc
      961 cggtaacggt tgccgtgcaa ggaggtctcg atgcgcgcct cggcgtcgaa gaacagcgcc
     1021 agccgttcgc ggatgttttc cagcgccatg cggttgccag cgcgctgctg ctcggcctcg
     1081 atataagggt tctcgatgcg cgcgatgacg cggtcgccgc gccgctcgat gcgcaccagc
     1141 acctcgcccg cggcggtatc gggctcgacg ccgtgataca cggcgttctc cagaagcggc
     1201 tgcagcacga gcggcggcag cagcgcgtcc gagggcgcgg cgtcgagctc ccaggtgatc
     1261 ctcagccgct cgcccagacg cagctgctcg agctctgcgt agcgctcgag cagcgcgatc
     1321 tcgtcggaga gccgcacgaa ctggcgcgcg tcggacatca gcgtgcgaaa cagatccgcg
     1381 aggtcttcga gcgagcgctc ggcgcgccgg gggtcgcgcc ggatgagcgc cagcacggca
     1441 ttcaggctgt tgaacagaaa atgcggccgg atcctcgcct gcagcgtctg cagccgcgcc
     1501 tcggccagcg cgggcgaaaa cgccttgacg agaaggcgga aatagccgag cagcccggcc
     1561 gcgagcgcgg cgcacagcac gatggtgcgc gcgaggagcg ccgcgccctg gggcacgccg
     1621 aacacggcca gctggtacgc cgcggcaagc gcggtcacga gcgccaccac cgcggcgcag
     1681 cccgcccaat acggcaaccg ggcgatccat ggcgacagcg cgaacagcgc aagcaactcg
     1741 aggaacagca gcggctcgac gaaagcggct gcctcgacga agcgatggag cgcgtcgcga
     1801 aaactcggcg ccgcgaacac cgcaccggcc agcgcaagcg cgttcaccgc caccagcacg
     1861 cgcgcgatga cgcccaggtt gcggaaatcc ggcagcacgt ccggataggc gttttgcctt
     1921 atactgacca tggttacgaa aaggtcttca accgcattct ccaagcatgg cgagaaagcc
     1981 gcaaccctgg tcgggccgct tcaaggatgc cgtcgacgag cgggtgaagc gctttaccgc
     2041 ctcggtgggc ttcgaccggc ggcttgcgcc ttacgacctc cagggctcac tcgcacacgc
     2101 gcgcatgctc gccgcctgcg gcattctgcc gcggcgcgac ctcaaggcaa tcgagcgcgg
     2161 gctcgcgcag atccgtcgcg agatcgagag cggtcgcttc gcatggtcga tcgacgccga
     2221 ggatgtgcat ctgaacatcg agcgccgcct cacggcgctc gtcggagagc ccggcaagcg
     2281 cctgcacacc gcgcgctcgc gcaatgatca ggtggcgacc gacctgcggc tgtggctgcg
     2341 cgccgagatc gacgccatcc gcaagctggt cgcggccctc gagcgctcgc tgctcgcgca
     2401 ggccgcgcgc catgccgcgc tgctgatgcc gggcttcacg cacctgcagg tcgcacagcc
     2461 gatcaccttc ggccaccacc tgctcgccta cgtcgagatg ctggagcgcg atcgtgaacg
     2521 gctgattcaa gttcgctcaa gagtaaatcg gttgccgctg ggcgcggccg cgctggccgg
     2581 caccagtttc cgcatcgacc gcgaacaggt ggcgcgcgag ctcggcttcg agggcgtgtg
     2641 cgagaactcg ctcgacgccg tgtccgaccg cgatttcgcc atcgagttcg cggcctgcgc
     2701 ggcgctcgtc atggtgcacc tgtcgcgcct ggcggaggaa ttggtgctct gggcgagccc
     2761 gcgcttcggc ttcgttcggc tgcccgagcg ctactgcacg ggctcctcga tcatgccgca
     2821 gaagaagaat cccgacgtgc cggagctggt gcgcggcaag agcggccggg tgttcggtga
     2881 tttggtggcc ctgctcaccc tgatgaaagg ccagccgctc gcgtacaaca aggacaacca
     2941 ggaagacaag gagccgctgt tcgacgccgc agccacggtg aagagctcgc tcggagtatt
     3001 cgctggcctg atcgccgatc tcgtgccggt gccccgtgcc atgcgcgccg cggcgctcga
     3061 gggccacgcc ac
//
LOCUS       DASPXW010000190         4982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_935844, whole genome shotgun sequence.
ACCESSION   DASPXW010000190 DASPXW010000000
VERSION     DASPXW010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935844"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..1449)
                     /gene="tldD"
                     /locus_tag="VEL04_04520"
     CDS             complement(19..1449)
                     /gene="tldD"
                     /locus_tag="VEL04_04520"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HYT47659.1"
                     /translation="MILETATATLLAPFDLEAGKLERVFAALGTRRIDYADLYFQYSR
                     FEGWSLEEGIVKSGSFNIEQGVGVRAVSGDKTAFAYSDEISFNALQDAAKATRAIASA
                     GQSRKTKAGPGKLPAPLYREVDPVASLGSAQKVALLEMLERKCRALDPRVSQVMASLG
                     GEYEVVLIARADGRIAADVRPLVRLSVQVIAEANGRRESGSSGGGGRTDYAHFTGELL
                     ERIARQAVSQALVNLEARAAPAGTMTVVLGPGWPGVLLHEAVGHGLEGDFNRKGSSAF
                     AGRLGQRVAAPGVTVVDDGTLPGRRGSLTVDDEGNPTQRNVLIEDGVLRTYMQDSLNA
                     RLLKMPLTGNARRESFAHLTLPRMTNTYMLAGPHAPEEIIASVQKGLYATNFGGGQVD
                     ITSGKFVFSAAEAYLIENGKVTHPVKGATLIGNGPDALTRVSMIGNDLELDWGIGTCG
                     KEGQSVPVGVGQPTLRIDGLTVGGTA"
     gene            complement(1446..2228)
                     /locus_tag="VEL04_04525"
     CDS             complement(1446..2228)
                     /locus_tag="VEL04_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HYT47660.1"
                     /translation="MTAVRMAAVQMVSAPEVGPNLEAAGRLIAAAAAAGARLVALPEN
                     FYLIGRDERDKVHAREPDGAGPIQQFLAETARKRGVWIVGGTAPISCEDAARIRSACL
                     VYDDAGKRVARYDKMHLFRFEHGAERYDESRTLEPGSAAVALASPFGRLALSVCYDVR
                     FPEIYRGLGEIDVVFVPSAFTVPTGTAHWETLLRARAIENQAYVVAPAQGGTHPSGRR
                     TYGHTMIIDPWGEILGVRPEGEGVVLAEIDPERPRQLRRRFH"
     gene            complement(2225..>4982)
                     /locus_tag="VEL04_04530"
     CDS             complement(2225..>4982)
                     /locus_tag="VEL04_04530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HYT47661.1"
                     /translation="FEPLAHLAEALPVPAELRSLIAELAPRGRLADAKFEWQGAVAAP
                     RRFSARTRFADLALRPWRGAPGFTGLTGNIEATEKKGRIHLAARNADVELPAVFPEPR
                     IGFDTLNAQVDWERQGESGFAVRFSSVSFTNPHASGNAYGSYAYSAAGPGTIDLAANL
                     TRADGAHIGKYLPLGRIMGERVREWLVEAIVGGQASDVRVRLKGDLREFPFTDPAKGQ
                     FLVAAHVERGVLNYANGWPRISDAEGELLFERDHMVIVGRSGSVLGAKLANVRVSLAG
                     LGAPAPHLLVNAQAEGPTGEFLKYIESSPVRRMTRGLTDGMSASGRGSLRLKLDLPLA
                     ELAASKVAGDYEFAANSVTVHHQLPAIERATGRFGFTDSGFTVHEVKGRLFGAPLQIS
                     GGSRPGGGIEIVARGRNVNVADLPMLEQPWRDQLSGAASYVATLSVREGATRLRLESN
                     LRGVASALPPPLDKSADEVLPLRLEATPGEGGARDRISLSLGRRAAAEILRRRQGETM
                     AVQRAAIWITPEAGQALRLPERPGTLIYGSLEAFDADPWLALLPAGGGAAGAGTAVDL
                     RIATLDAYGKRLHHVALRAGSDAVGWTASVKADELSGDLSYRGEQGGKLVARLDHVSI
                     PPDSPGPKRERGIRPADLPALDLVAERFDFRGKELSHFELAASRVGEDWRIEKLAMAN
                     PEATLRATGLWRNGSGGAPSRSSLEFELDTSNAGEFLARVGYPNVVKGGKANLQGSLA
                     WQGEPAAIDFPTLGGELKLQADDGQFLEIQPGFGKLISLMSLQALPRRIALDFRDVFS
                     KGFQFDHIAAEARVEDGLMGIKELAMRGSAAEVQMTGEVDLARETQNLNVRVVPGLGD
                     SAATALVIVNPVAGVAAAIAQRVLKNPLGQIFAYDYAVTGSWSDPKVAKVSTPRPAQE
                     SSTP"
BASE COUNT          716 a         1803 c         1681 g          782 t
ORIGIN      
        1 tccattggtg tgatgcgtct aagccgtgcc gcccaccgtc aagccatcaa tgcgcagcgt
       61 cggctggccg acgccgaccg gcacgctctg gccttccttg ccgcaggtgc cgatgcccca
      121 gtcgagctcg aggtcgttgc cgatcatgct gacacgcgtc agcgcatcgg gcccgttgcc
      181 gatcagggtc gcgcccttga ccgggtgggt caccttcccg ttctcgatca ggtaggcctc
      241 ggcggcggag aagacgaatt tgccgctggt gatgtcgacc tggccgccgc cgaagttggt
      301 ggcgtacagg cccttctgca cgctggcgat gatctcctcg ggcgcgtgcg gcccggcgag
      361 catgtaggta ttggtcatgc gcggcagcgt gagatgcgcg aacgactcgc ggcgcgcgtt
      421 gccggtgagc ggcatcttca ggagccgcgc gttgaggctg tcctgcatgt aggtgcgcag
      481 cacgccatcc tcgatcagca cgttgcgctg cgtcggattg ccttcgtcgt cgaccgtgag
      541 cgagccgcgg cggccgggaa gcgtgccatc gtcgaccacc gtgaccccgg gcgcggcgac
      601 gcgctggccg aggcggcccg cgaacgccga gctgcccttg cggttgaagt cgccctcgag
      661 gccgtgcccg accgcctcgt gcagcagcac gccgggccag cccggaccca gcactaccgt
      721 catcgtgccg gccggcgcgg cacgcgcctc gaggttgacg agcgcctgcg aaaccgcctg
      781 gcgcgcaatg cgctcgagca gctcgccggt aaagtgcgca tagtcggtgc ggccgccacc
      841 gccggaggat ccgctctcgc ggcggccgtt tgcttccgcg atcacctgca ccgacagccg
      901 caccagcggc cgtacgtcgg cggcaatcct gccgtcggcg cgcgcgatca gcaccacctc
      961 gtactcgccg ccgaggctcg ccatcacctg gctgacgcgc gggtcgagcg cgcggcattt
     1021 gcgctcgagc atctccagaa gcgcgacctt ttgcgccgac ccgagcgagg cgaccgggtc
     1081 gacctcgcgg tatagaggcg caggcagctt gcccggacct gcttttgttt tcctggattg
     1141 acccgcagaa gcaattgcac gcgttgcctt cgccgcgtcc tgcagcgcgt tgaagctgat
     1201 ctcgtcggaa taggcaaagg cggtcttgtc gcccgacact gcgcgcacgc ccacgccctg
     1261 ctcgatgttg aagctgcccg acttgacgat gccctcttcc aggctccagc cctcgaagcg
     1321 cgaatactgg aaatacaggt cggcgtagtc gatgcggcgc gtgccgagcg ccgcgaacac
     1381 gcgctcgagc ttgcctgcct cgagatcgaa gggcgcgagc agcgtcgctg ttgcggtttc
     1441 gaggatcaat ggaatctcct tcgcaattgt cgcgggcgct cggggtcgat ctcggcgagc
     1501 accacgcctt cgccttccgg gcgcaccccg aggatttctc cccaggggtc gatgatcatg
     1561 gtgtggccat aggtgcgccg cccgctcggg tgagtgccgc cctgcgccgg cgcgacgaca
     1621 taagcctggt tctcgatggc acgcgcgcgc agcagcgtct cccagtgcgc ggttccggtc
     1681 ggcacagtga acgccgaggg cacgaacacc acgtcgatct ctcccagtcc ccggtagatc
     1741 tcgggaaagc gcacgtcgta gcacaccgaa agcgccagcc ggccgaacgg gctcgccagc
     1801 gccaccgcgg cgctgcccgg ctcgagggtg cgggactcgt cgtagcgctc ggcgccgtgc
     1861 tcgaagcgga acagatgcat cttgtcgtag cgggcgaccc gcttgccggc gtcatcgtag
     1921 accaggcagg cgctgcggat gcgggccgca tcctcgcagg atatgggggc ggtgccgccg
     1981 acaatccaga ccccgcgttt tcgggcggtt tcggcaagaa attgctgtat cgggccggcg
     2041 ccatccggct cgcgcgcgtg caccttgtcg cgctcgtcgc gcccgatcag gtagaaattc
     2101 tccggcagcg ccaccaggcg agcacccgct gcggccgccg cggcgatcag ccggcccgcg
     2161 gcctcgaggt tcgggccgac ctcgggcgca gagaccatct gcactgccgc catcctcacg
     2221 gcggtcacgg cgtcgaactc tcctgggccg gccgcggtgt ggaaaccttc gccaccttcg
     2281 gatcgctcca gctcccggtt acggcgtagt cgtaggcgaa aatctgcccc agcgggttct
     2341 tcagcacgcg ctgcgcgatg gccgcggcga cgcccgccac cgggttcacg atcaccagcg
     2401 cggtcgccgc gctgtcgccc agcccgggaa ccacgcgcac gttcaggttc tgcgtctcgc
     2461 gcgcgaggtc gacctcgccc gtcatctgca cttccgcggc cgagccgcgc atcgccaatt
     2521 ccttgatgcc catcaggccg tcctcgacgc gcgcctcggc ggcgatgtga tcgaactgga
     2581 agcccttcga gaacacgtcg cgaaagtcga gcgctatgcg ccgcggcagc gcctgcaggc
     2641 tcatgagcga gatcagcttg ccgaagcccg gctggatctc gaggaactgg ccgtcgtcgg
     2701 cctgcagttt cagctccccg ccgagcgtcg ggaagtcgat cgccgcgggc tcgccctgcc
     2761 atgcgagcga gccttgcagg ttcgctttcc cgcccttcac cacgttggga tagccgaccc
     2821 gcgcgagaaa ctcgccggcg ttgctcgtgt cgagctcgaa ctcgaggctg ctgcgcgaag
     2881 gtgcgccgcc gctgccgttc ctccacagtc cggtcgcacg caacgtcgcc tccgggttgg
     2941 ccatcgccag cttctcgatg cgccaatcct cgcccacgcg gctcgccgcg agctcgaagt
     3001 ggctgagctc cttgccgcgg aaatcgaagc gctcggcgac gaggtccagc gcaggcaagt
     3061 cggccggccg tattccccgc tcgcgcttcg gtccggggct gtcaggcggt atcgacacgt
     3121 gatcgagccg cgcgacgagc ttgccgccct gctcgccgcg ataggacagg tcacccgaga
     3181 gctcgtccgc cttcacgctc gccgtccagc cgacggcgtc ggatccggcg cgcagcgcca
     3241 catggtgcag ccgcttgccg taggcatcga gcgtcgcgat acgcaggtcg acagcggtgc
     3301 ctgcgcctgc tgcgccgccg cccgcaggca ggagcgcgag ccaggggtcg gcgtcgaacg
     3361 cctcgagcga gccgtagatc agcgtgccgg ggcgctcggg aagccgcagc gcctgccccg
     3421 cttcgggcgt aatccagatc gcggcacgct gcaccgccat ggtctcgccc tgccggcggc
     3481 gcaggatttc ggcagcggcc cgccgcccga ggctgagcga gatccggtcg cgcgcgccgc
     3541 cttcgcccgg tgttgcctcg aggcgcagcg gcagcacttc gtccgcgctc ttgtcgagcg
     3601 gcggcggcag ggcgctcgcc acgccccgca agttcgattc gagcctcagg cgcgtcgcgc
     3661 cctcgcgcac gctgagcgtc gccacgtagg aagctgcgcc gctcagctga tcgcgccagg
     3721 gctgctcgag catcggcagg tcggcgacat tgacgttgcg cccccgcgcc acgatctcga
     3781 tgcccccgcc ggggcgcgag ccgccgctga tctggagggg cgcgccgaac aaccgccctt
     3841 tcacctcgtg caccgtgaag cccgagtcgg tgaagccgaa cctgcccgtg gcgcgctcga
     3901 tcgccggcag ctgatggtgc acggtgacgc tgttcgcggc gaactcgtaa tcgcccgcca
     3961 ccttgctcgc cgccagctcg gcgagcggca ggtcgagctt gaggcgcagc gacccccgcc
     4021 ccgacgcgct catgccgtcg gtcaggccgc gggtcatgcg gcgcaccggc gaggactcga
     4081 tgtacttgag gaattcgccg gtcgggcctt cggcctgcgc attgacgagc aaatgcggcg
     4141 ccggcgcgcc caggccggcg aggctgaccc gcacgttggc gagcttcgcg ccgagaacgc
     4201 tgccgctgcg gccgacgatc accatgtggt cgcgctcgaa cagcagctcg ccttcggcgt
     4261 cgctgatgcg cggccagccg ttcgcgtagt tcaggacgcc gcgctccacg tgcgcggcca
     4321 ccagaaactg gcccttggcc ggatcggtga acggaaactc gcgcaggtcg cccttcagcc
     4381 gcacgcgcac atcgctcgcc tgcccgccga cgatcgcctc gaccagccac tcgcgcacgc
     4441 gctcgcccat gatgcgaccg agcggcaggt acttcccgat gtgcgcgccg tccgcgcgcg
     4501 tcaggttcgc cgcaaggtcg atggtcccgg gcccggccgc gctgtaggca tacgacccgt
     4561 aggcattgcc gctcgcgtgg ggattggtga agctcaccga cgaaaagcgc accgcaaagc
     4621 cgctttcgcc ctggcgctcc cagtcgacct gcgcgttcag cgtgtcgaac ccgatgcgtg
     4681 gctcggggaa cacggccggg agctcgacgt cggcgttgcg cgcagcgaga tggatgcggc
     4741 ccttcttctc ggtagcctcg atgttgccgg tgaggcccgt gaatcccgga gcgccacgcc
     4801 agggtcgtaa cgccaagtcg gcaaaccgcg tccgcgcgct gaagcgccgc ggtgccgcga
     4861 ccgcgccctg ccattcgaat ttcgcgtccg ccagccgccc gcgcggcgcc agctcggcga
     4921 tcagcgagcg cagctcggcg ggaaccggca gcgcctcggc gagatgggcg agcggctcga
     4981 ac
//
LOCUS       DASPXW010000191         7504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_560097, whole genome shotgun sequence.
ACCESSION   DASPXW010000191 DASPXW010000000
VERSION     DASPXW010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7504
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560097"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1418)
                     /locus_tag="VEL04_04535"
     CDS             complement(<1..1418)
                     /locus_tag="VEL04_04535"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HYT47662.1"
                     /translation="MLDKLIATCVHRRVAAVVTIAILAAFGVRAYLNTPIEAYPDVTN
                     AQVTVIAQMPGYAPEEIERSVTVPLERALNGTPRTQQLRSESLFGLSLVTLTFDDDAD
                     PFISRMHVAQRMAAADLPDGVTPMLAPEATPLGEVYQFRVVSERHDLYQTRAELQWTV
                     SRVLKQVPGVADVVCFGGYLKEVHVRAYPERMRAHGVTLPELAEALAKTNINIGGGFL
                     RHGDQELTVRGMGYINSVEDIRRTALKTREGTPVMVGDVAEIVQSNTPRRGAVGWNEQ
                     KEAVEGFVLMRRGENPSRVLDGVHQKVAELNGKILPRGMTIEPFYDRTLLVGNTLKTV
                     NKNLLHGFILVVAIAWLFFRSITGSLIVGVVIPLALLGAFLGLYALKLPANLISMGAI
                     DFGILVDGAVVLVENVLHSVQHERPARRSDLLRLVIRAAEQVARPTLYAMLIIIAALV
                     PVFTLQQVEGRIFRPLALTYSFA"
     gene            complement(1419..2534)
                     /locus_tag="VEL04_04540"
     CDS             complement(1419..2534)
                     /locus_tag="VEL04_04540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HYT47663.1"
                     /translation="MLVHPRPAVWVLVFCLCACGDKAAAPKRTQVAGTTPAAGAVALA
                     EVSRQFLTIEALEASAGTGARSYFGRTAFRPKALSAVTAPFSGRVAAVAVEPGQKVRA
                     GATLFTIESADAGGVRAALEQARIKVRVAREVLARQTEMVKRGVGLEVERFDAEMKLR
                     EARSELERSERNAALLGRGQGTAVNVRAPVDGVIVSVKAAPGATVQAGGEPLVEIGNP
                     GGLWVVADVPEGEAAQAAKAKSAEVLVSALNIKLAGRIAGIAPRSDPETRRTPIYVEL
                     ESPPAELRAGLLVRVNVAVDASAGELWLPVTAVLLKEGGKRVVYVEQSDGRLVAREIE
                     AGDERGGRVRVLKGLEPGERVVMKGALLVDREAEQLL"
     gene            2632..3273
                     /locus_tag="VEL04_04545"
     CDS             2632..3273
                     /locus_tag="VEL04_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019042998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47664.1"
                     /translation="MRTAILIACIALICARQARAARPFFTDDARVVDEGHCQLETFYK
                     EQHAYNASEFWFLPACNPAFVPLLSGVELTAGGNRIEGERNFILQGKKLFKTLETNGQ
                     GYAASLGVFGGDPYVNGIASFSFLDDRAVVHTNLGLIKDKGTWGIGLEALLAAPRLYG
                     ILETFGQRGDTPTQHAGLRFWVIPNRFQIDATAGRQSGWPERRFTSVGLRFLF"
     gene            complement(3315..4604)
                     /locus_tag="VEL04_04550"
     CDS             complement(3315..4604)
                     /locus_tag="VEL04_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HYT47665.1"
                     /translation="MLTREQNELLCRVEGEAAMGQMMRRHWLPVCMAEEVAARDGAPA
                     RARLLGEDLVVFRDTDGKLGVLGEHCLHRRASLAFGRNEECGLRCLYHGWKFDVEGNV
                     VDMPSEAPGAAQRLGKKTRCYPAREGGGFIWIWMGPRDQMREWEPPAWAPAAEARTSI
                     VKMHVGCNWAQVLEGSIDSAHSSSLHSTNMPAAEVDGAKATATAWLRPSIDKAPRLQF
                     EPTSYGFRYAAIRKPIRNPETDQYVRTTLFIAPFTVLIPPNDRYNLAQMLVPLDDVNT
                     MFYWVAWHPDAAKGISQREWRRFCAAEVGVDLDENYRKKRNLQNRYLQDRQAMQRGDF
                     TGIQGIPAQDMAMWESMGPITDRSQDHPGSSDLAVAQFRRVMVAAAQRFQEGGPAIGT
                     AEPRIPQVKLASFEGIVPKTTDWRTLGVPSEEAMTAK"
     gene            4647..5819
                     /locus_tag="VEL04_04555"
     CDS             4647..5819
                     /locus_tag="VEL04_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYT47666.1"
                     /translation="MRKLKIGAAGLGRAFGLMAPTLARDARLQLVAAADPRPEALQQI
                     HKDFGARGFTSLESLLKESDAEVLYIATPHQFHAEHACLAASHGKHLLVEKPMALTLK
                     DCRAMIAAARKAGVQLIVGHSHSFDRPILETRKIIEQGAVGQLRMITAINFTDFLYRP
                     RRPEELDTARGGGAMFSQAAHHVDVVRLLGGGRVKSVRAATGRWDPSRPTEGAYACFL
                     TFKDGAVASMVYSGYAHFDSDEFCGWIGEVGQKKDPAQYGAARRFLSAGTDELALKHV
                     RNYGAPGFVPPEERPLHQHFGPLIVSCDKADLRPLPTGVMVYADGVQRLEALPAPDVP
                     RAEVIDELYEAVVNGKAPLHSGEWALATLEVILAMLRSAGEGKEIALKSQTSASLT"
     gene            5843..6232
                     /locus_tag="VEL04_04560"
     CDS             5843..6232
                     /locus_tag="VEL04_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HYT47667.1"
                     /translation="MRALQPRLAGHAVPFGHWTFLRILWEQDGITQRELSDEAGVMEP
                     TTFAALKAMEELGYVTRRQTAENRKNVYVYLTPKGRALRSKLMPLAVEVNAIATGEIS
                     ATDLAVTRRALLAMIDNLSRSSPRRPR"
     gene            6229..7221
                     /locus_tag="VEL04_04565"
     CDS             6229..7221
                     /locus_tag="VEL04_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYT47668.1"
                     /translation="MKKLRLTLACWDYDRTRALSDGTVRAEGIDLIYLTQPVEETFFR
                     MMRHREFDCSEMSLSSYVASLNSQDPPFIAVPVFPSRFFRHSCIFVSAKSGIRKPEEL
                     KGKRIGVPEYQMTAPVWIRGILSDDYGVKVTDLEHLAGGEEEPGRDEKLKLDLPSSIR
                     LKAIGPTQTLSQMIAQGEIDALVTARAPSTFRSKPDAVKRLFPSYVELEKEYYRRTKI
                     FPIMHTLVIRRDVYAANPWVAQSLYKAFVAAKAKAYALYDQTAALPAMVPWLVPQLEE
                     ARREMGEDWWPYGLEPNRHVLETFLRYHHEQGLSKRRFTPEELFARETLQTFKV"
     gene            complement(7270..>7504)
                     /locus_tag="VEL04_04570"
     CDS             complement(7270..>7504)
                     /locus_tag="VEL04_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HYT47669.1"
                     /translation="GAPPVEVRVRPGELRVCDAGPGVPEAERERIFQPFHSSRGTGLG
                     LSLVRQIARRHGGEARCEGNCFVVTLRSSVPTG"
BASE COUNT         1263 a         2580 c         2428 g         1233 t
ORIGIN      
        1 gcaaacgaat aggtcagggc gagcggccgg aagatgcggc cttcgacctg ctggagcgtg
       61 aataccggca ccaacgccgc gatgatgatc agcatcgcat aaagcgtcgg ccgcgcgacc
      121 tgctcggcgg cccggatcac gaggcgcagc aggtcactgc gcctcgccgg acgctcgtgc
      181 tgcacgctgt ggagcacgtt ctcgaccagc accaccgcgc cgtcgaccag gatgccgaag
      241 tcgatcgcgc ccatcgagat caggttcgcc ggcagcttga gcgcgtacag cccgaggaac
      301 gcgccgagca gcgcgagcgg gatcaccacc ccgacgatca gcgagccggt gatgctgcgg
      361 aagaacaacc aggcgatcgc caccaccagg atgaagccgt gcagcaggtt cttgttcacc
      421 gtcttgagcg tgtttccgac caggagcgtg cggtcgtaga acggctcgat cgtcatgccg
      481 cgcggcagga tcttgccgtt cagctcggcg accttctggt ggacgccgtc gagcacgcgc
      541 gacgggttct caccgcggcg catcagcacg aagccttcga ccgcctcctt ctgctcgttc
      601 cagccgacgg cgccgcgccg cggcgtgttc gactgcacga tctcggcgac gtcgccgacc
      661 atcaccggcg tgccctcgcg cgtcttgagc gccgtacgcc tgatgtcctc gaccgagttg
      721 atgtaaccca tgccgcgcac cgtgagctcc tggtcgccgt ggcgcaggaa gccgccaccg
      781 atgttgatgt tggtcttggc gagcgcctcg gcaagctcgg gcagcgtcac gccgtgcgcg
      841 cgcatgcgct cggggtaggc acgcacgtgc acttccttga gatagccgcc gaagcacacc
      901 acgtcggcga cgcccggcac ttgcttcagc acgcgactta ccgtccactg cagctcggcg
      961 cgcgtctggt aaaggtcgtg ccgttcgctg accacgcgga actggtacac ctcgccgagc
     1021 ggcgtggcct ccggggcgag catcggcgtc accccgtcgg gcaggtcggc ggcggccatg
     1081 cgctgcgcga catgcatgcg cgagatgaac gggtcggcgt cgtcgtcgaa ggtgagggtc
     1141 accagtgaca gcccgaacag gctctcgctt ctgagctgct gggtgcgcgg cgtgccgttg
     1201 agcgcgcgct cgagcggcac ggtgacgctg cgctcgattt cctcgggggc gtagcccggc
     1261 atctgcgcga tcaccgtcac ctgcgcgttg gtaacgtcgg gataggcctc gatcggcgtg
     1321 ttgaggtagg cgcgcacgcc gaacgcggcg aggatggcga tggtcacgac cgccgcgacg
     1381 cggcggtgta cgcaggtggc gatcagcttg tcgagcatct agagcagctg ctccgcctcg
     1441 cggtcaacga gcagcgcgcc cttcatcacc acccgctcgc ccggttccag ccccttcagc
     1501 acgcgcacgc gcccgccgcg ctcgtcgccg gcctcgatct cgcgcgcgac caagcgcccg
     1561 tcgctttgct cgacgtagac cacgcgcttg ccgccttcct tgaggagcac cgcggtcacc
     1621 ggcagccaca gctcgcccgc gctcgcgtcg accgcaacgt tgacgcgcac cagcaacccg
     1681 gcgcgcagct ccgccggcgg gctctccagc tcgacgtaga tcggcgtacg gcgcgtctcg
     1741 gggtcgctgc gcggcgcgat gccggcgatc cttcccgcga gcttgatgtt cagcgcgctg
     1801 accaggactt cggcgctctt cgccttggcg gcctgcgccg cctcgccttc gggaacgtcg
     1861 gcgacgaccc acagcccgcc gggattgccg atctcgacca gcggctcgcc gcccgcctgc
     1921 accgtcgcgc ccggcgcagc cttgacgctg acgatgacgc cgtcgaccgg cgcgcgcacg
     1981 ttcaccgccg tgccctgccc cctgccgagg agcgccgcgt tgcgctccga gcgctcgagc
     2041 tccgagcgcg cctcgcgcag cttcatctcg gcgtcgaagc gctccacctc gaggccgacg
     2101 ccgcgcttca ccatctcggt ctggcgcgcg agcacctccc gggcgacgcg caccttgatg
     2161 cgcgcctgct cgagcgccgc gcgcacgccg cccgcgtccg cgctctcgat cgtgaacagc
     2221 gtggccccgg cgcgcacttt ctgtcccggc tcgaccgcca ccgcggcgac gcggcccgag
     2281 aacggcgcgg tcaccgcgga gagcgctttc gggcggaagg cggtgcggcc gaagtagctc
     2341 cgcgcgcccg tgccggcgct cgcttcgagc gcctcgatgg tgaggaactg gcgcgagact
     2401 tcggcgagcg cgaccgcacc cgcagcgggc gtggtgccag cgacttgcgt gcgcttcggc
     2461 gcagcggcct tgtcgccaca cgcgcacaaa caaaacacga gcacccagac tgcgggtctc
     2521 gggtgcacca gcattcacgc cccccatttt gaccgggggc gcattttaag ctattgaaag
     2581 aaattagaat tgacaggaaa acgtcaggtt cggccagaaa agaacaacgg cttgcgcact
     2641 gcaatactca ttgcctgcat cgccctgatc tgcgcgcggc aagctcgcgc cgcgcggccg
     2701 ttcttcaccg acgatgcgcg cgtcgtcgat gaaggccact gccagctcga gaccttctac
     2761 aaggagcagc acgcctacaa cgcgtccgaa ttctggtttc tgccggcgtg caatcctgcc
     2821 tttgttcctc ttctgtcggg cgtggagctc accgcgggcg gcaaccgcat cgagggcgag
     2881 cgcaatttca tcctgcaagg caagaagttg ttcaagaccc tggagaccaa cggccagggc
     2941 tacgcagcat cgctcggtgt cttcggcggc gatccgtatg tcaacggcat cgccagcttc
     3001 tcctttctcg acgatcgcgc cgtcgtgcac accaacctcg gtctcataaa agacaagggc
     3061 acctggggga tcggcctcga agcgctgctc gccgcgccgc ggctctacgg catcctcgag
     3121 accttcggcc agcgcggcga cacgccgacg cagcacgcgg gcttgcgttt ctgggtgatt
     3181 cccaaccgct tccagatcga cgccactgca ggcaggcaga gcgggtggcc cgagcggcgc
     3241 ttcaccagcg tcgggctgcg attcctgttt tgattcgagg cgcgctatcg acagcgctgt
     3301 cgccgcgata ggatctactt cgcggtcatt gcctcttcag aaggtacgcc aagcgtgcgc
     3361 cagtcggtgg tcttcggcac gatgccttcg aacgaggcga gcttgacttg cggaatgcgc
     3421 ggctcggcgg taccgatcgc cggcccgccc tcctggaagc gctgcgccgc ggcgaccatc
     3481 acacggcgga actgcgccac cgcgagatcg ctcgagccgg gatggtcctg gctgcggtcg
     3541 gtgatcggcc ccatcgattc ccacatcgcc atgtcctggg ccgggatgcc ctggataccg
     3601 gtgaaatcgc cgcgctgcat tgcctgccga tcctgcagat agcggttttg cagatttctt
     3661 ttctttctgt agttttcatc caggtcgacg ccgacttccg cggcgcagaa gcggcgccat
     3721 tcccgttgcg agatgccttt cgccgcatcc ggatgccagg ccacccagta gaacatggtg
     3781 ttgacgtcgt cgagcggcac cagcatctgc gcgaggttgt agcggtcgtt cggcggaatc
     3841 aggacggtga acggcgcgat gaacagcgtg gtgcggacgt actggtcggt ctcggggttg
     3901 cggatcggct tgcggatcgc cgcgtagcgg aagccgtagc tcgtcggctc gaactgcagc
     3961 cgcggcgcct tgtcgatcga tgggcgcagc caggcggtag cggtcgcctt ggcgccgtcg
     4021 acctcggccg cgggcatgtt ggtcgagtgc aggctcgagc tgtgcgcaga atcgatcgag
     4081 ccttctagta cttgtgccca gttacacccg acgtgcatct tgacgatcga ggttcgagct
     4141 tccgcagcgg gcgcccaggc aggcggctcc cattcgcgca tctggtcgcg cgggcccatc
     4201 cagatccaga tgaaaccgcc gccttcacgc gccgggtagc acctggtctt cttgccgagc
     4261 cgctgcgcgg cgcccggcgc ctccgaaggc atgtcgacga cattgccctc gacgtcgaat
     4321 ttccagccgt gatacaggca gcgcaggccg cattcctcgt tgcggccgaa ggcgagcgat
     4381 gcgcgacggt gcaggcaatg ctcgcccaga acgccaagct tgccgtcggt atcgcggaat
     4441 accaccaggt cttcgccgag cagccgggcg cgcgctggcg cgccatcgcg cgcggcgacc
     4501 tcttctgcca tgcagaccgg gagccagtgc cgccgcatca tctgtcccat ggccgcttcg
     4561 ccttcgacgc ggcacagcaa ctcgttctgc tcccgggtca gcatggcgta tacttagccc
     4621 cctaagcatt taggatacgc ccggagatga gaaagctcaa gatcggcgct gccggacttg
     4681 gccgcgcctt cggcctgatg gcgcccacgc tggcgcgcga tgcgcgcctg caactggtcg
     4741 ccgccgcgga tccccggccg gaagccttgc agcaaataca caaggacttc ggcgccaggg
     4801 gctttacctc gcttgaatcc ctgttgaagg aaagcgacgc cgaggtgctc tacatcgcca
     4861 cgccgcacca gttccacgcc gagcacgcct gcctcgctgc ctcgcacggc aagcacctgc
     4921 tggtcgagaa gccgatggcc ctcacattga aggattgccg cgccatgatc gcggccgcgc
     4981 gcaaggcggg cgtgcagctc atcgtcggcc acagccacag cttcgacagg ccgatcctgg
     5041 aaaccagaaa aatcatcgag cagggcgcgg tgggccagct gcgcatgatc accgcgatca
     5101 acttcaccga tttcctctac cgtccgcggc ggcccgaaga gctcgacacc gcacgcggcg
     5161 gcggcgcgat gttcagtcag gccgcgcacc atgtcgatgt ggtgcggctg ctcggcggcg
     5221 gccgggtaaa gagcgtgcgc gcggcgaccg gcagatggga cccttcccgc ccgaccgaag
     5281 gggcttatgc ctgttttctg actttcaagg atggagcggt cgcctcgatg gtgtacagcg
     5341 gctatgcgca cttcgactcc gacgaattct gcggctggat cggcgaggtc ggccagaaaa
     5401 aggacccggc acaatacgga gcagcgagaa gatttctctc tgcggggaca gacgaactgg
     5461 cattgaaaca cgtgcgcaac tacggcgcgc ccggcttcgt gccgccggag gagcgaccgc
     5521 tgcaccagca cttcggcccg ctcatcgtct cgtgcgacaa ggcggacctg cggccgctgc
     5581 ctaccggcgt gatggtctac gccgatggcg tgcagcggct cgaggcgctt cccgccccgg
     5641 atgtgccgcg cgccgaagtg atcgacgagc tctacgaggc ggtggtgaac ggcaaagcgc
     5701 cgctgcacag cggcgaatgg gcgctcgcga cgctggaggt gatcctcgcg atgctgcgct
     5761 cggcaggcga aggaaaggaa atcgcattaa aaagccagac gagcgcctcg ctcacctgat
     5821 caagcacgcc tggcgggcgt tcgtgcgcgc gctgcagccg cgcctcgccg ggcacgcggt
     5881 gccgttcggg cactggactt tcctccgcat cctgtgggag caggacggca tcacgcagcg
     5941 cgagctctcc gacgaggccg gcgtgatgga gccgaccacc ttcgcggcgc tcaaggcgat
     6001 ggaggagctc ggctacgtca cgcgccggca gacggccgag aaccgcaaga acgtctatgt
     6061 ctacctgacg ccgaagggcc gcgcgctgag gagcaagttg atgccgctcg cggtcgaagt
     6121 caacgctatc gcgaccggtg agatcagcgc cacggacctt gccgttacgc ggcgcgcgct
     6181 gctcgcgatg atcgacaacc tcagtcgatc ttcgccccgg agaccgcgat gaaaaagctg
     6241 cggctcacgc tcgcctgctg ggactacgac cgcacgcgcg cgctcagcga cggcactgtt
     6301 cgcgcagaag gcatcgacct catctatctc acgcagccgg tcgaagagac tttcttccgc
     6361 atgatgcgcc accgcgagtt cgactgctcc gagatgtcgc tctcctccta tgttgcttca
     6421 ttgaacagtc aagacccgcc tttcatcgca gtcccggttt tcccctcgcg cttcttcagg
     6481 cactcgtgca ttttcgtcag cgcgaaaagc ggcatccgca agccggaaga actgaaaggc
     6541 aagcgtatcg gcgttcccga gtaccagatg accgcgccgg tctggatccg cggcatcctg
     6601 tcggacgatt acggcgtcaa ggtcacggac ctcgagcacc tggcaggcgg cgaggaggag
     6661 cccgggcgcg acgaaaagct caagctcgac ctgccttctt ccataagact aaaagcgatc
     6721 ggtccaacgc aaacgctgtc gcagatgatc gcgcagggcg agatcgatgc gctcgtaacg
     6781 gcgcgtgcgc cctcgacgtt ccgctcgaaa ccggacgccg tgaaaaggct gtttccgagc
     6841 tacgtggagc tcgaaaagga gtattaccgc cgcaccaaga tattcccgat catgcacacc
     6901 cttgtcatcc ggcgcgacgt ctacgccgcg aatccctggg tggcgcaatc cctctacaag
     6961 gccttcgtcg cggcaaaggc aaaggcctac gcgctctacg accagaccgc ggcgctgccg
     7021 gcgatggtgc cgtggctcgt gccgcagctc gaggaagcgc ggcgcgagat gggcgaggac
     7081 tggtggcctt acggcctgga gccgaaccgg cacgtgctcg agacgttcct gcgctatcac
     7141 cacgagcagg ggctgtcgaa gcgccgcttc acccccgaag agctgttcgc gcgcgagacg
     7201 ctgcaaacct tcaaggtcta attacgtaaa tacgccgtcc gtcgcactcg gcgcgcctca
     7261 ccagccaagt cagccggtgg gaactgaaga acgcagcgtg acgacgaagc agtttccttc
     7321 gcaacgcgcc tcgccgccgt gccggcgcgc gatctggcgc acgagcgaca ggccaaggcc
     7381 ggtgccgcgg ctggagtgga acggctggaa gatgcgctcg cgctcggctt cgggaacccc
     7441 gggcccggcg tcgcagacgc gcagctcgcc ggggcgcaca cgcacttcga ccggcggcgc
     7501 gccg
//
LOCUS       DASPXW010000192         2715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1819685, whole genome shotgun sequence.
ACCESSION   DASPXW010000192 DASPXW010000000
VERSION     DASPXW010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1819685"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1536)
                     /gene="nusA"
                     /locus_tag="VEL04_04575"
     CDS             complement(67..1536)
                     /gene="nusA"
                     /locus_tag="VEL04_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HYT47670.1"
                     /translation="MLVDVLAREKNVPRDIVFGALEMALASATKKKYSEDIDVRVQVD
                     RNTGAYESFRRWKVVPDEAIESPPAQIALSEAQKRKADVKLEDFIEEPLEPIEFGRIG
                     AQTAKQVILQRIRDAEREQILNDFLSRGDELVTGTVKRMERGNAIIESGRLEALLPRE
                     HMIPKENLRVGDRVRAWVMKIDRQARGPQLILSRTAPQFIMKLFELEVPEIEEGLLEI
                     KSAARDPGIRAKIAVFTNDKRIDPIGTCVGMRGSRVQAVTQELAGERVDIVLWAADPA
                     QFVIGALAPAEVSSILVDEEKHAMDVVVDEENLAIAIGRSGQNVRLASELTGWQINLM
                     TEEESTKKQQEESGRIKQHFMERLDVDEDVANILIEEGFSTLEEVAYVPINELNEIEA
                     FDEQTVNELRNRARNALLVAAIANEEKMEGVDPELLKLEGMDTTLAAKLAEKGVKTRD
                     DLADLAVDELAEMTGLDEEKSKKLILAARAHWFASKESA"
     gene            complement(1589..2023)
                     /gene="rimP"
                     /locus_tag="VEL04_04580"
     CDS             complement(1589..2023)
                     /gene="rimP"
                     /locus_tag="VEL04_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HYT47671.1"
                     /translation="MAGIDSVVEPVVAGMGFELVDAQASNGGRLLRLFIDKPGGVGLE
                     DCAAVSRQLTRVLEVEGIDYERLEVSSPGLDRRLRKAGDFARFSGHKAEVRMKTPDAT
                     GRRRFVGVLRGAEAGLVNMELEGQTVRLALEDVDRAKLIPEL"
     gene            complement(2112..>2715)
                     /locus_tag="VEL04_04585"
     CDS             complement(2112..>2715)
                     /locus_tag="VEL04_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HYT47672.1"
                     /translation="AVEDDEIAVDGNPVAWLPAKSAPRVLLYHKRVGELVTRSDPEGR
                     PTVFSRLPAGRWVAVGRLDINSSGLLLLTNSGELANRLMHPRYEIEREYAARVQGRLG
                     PEALEKLKLGVQLEDGLAAFTCIEFMEKNAGAEGANRWYRVVLREGRNREVRRLFEAV
                     GARVSRLMRVRYGPVELPRDLPAGRWRELAAQPLQRLLRH"
BASE COUNT          416 a          965 c          816 g          518 t
ORIGIN      
        1 tcgagcagca ccgaaggcgg gaccttcagc tcgctcgcga actgagctac gctcgtttgc
       61 gccacgttat gccgactcct tgcttgcaaa ccagtgggcg cgggcagcca ggatcagctt
      121 cttcgacttc tcttcatcca gcccggtcat ttcggccagc tcatcgacgg cgagatcggc
      181 gagatcgtcg cgcgtcttga cgcccttctc cgcgagcttg gcggcgagcg tcgtgtccat
      241 gccctcgagc ttgagcagct ccgggtcgac gccttccatc ttctcctcgt tggcgatcgc
      301 cgccacgagc agcgcgttgc gcgcgcggtt gcgcagctcg ttgacggttt gctcgtcgaa
      361 ggcttcgatc tcgttgagct cgttgatcgg cacgtaggcc acttcctcga gcgtcgaaaa
      421 accttcctcg atcaggatgt tggcgacgtc ctcgtcgacg tccaggcgct ccatgaaatg
      481 ctgcttgatg cggccgctct cctcctgctg cttcttggtc gactcttcct cggtcatcag
      541 gttgatctgc cagccggtca gctcggaggc gaggcgcaca ttctggccgc tacggccaat
      601 cgcgatcgcg agattttcct cgtccacgac cacgtccatt gcgtgcttct cttcgtccac
      661 caggatcgag ctgacctcgg cgggagccag cgcaccgatc acgaactgcg cggggtccgc
      721 cgcccacagc acgatgtcga cgcgctcgcc cgcgagttcc tgcgtgacgg cctggacgcg
      781 cgagccgcgc atgccgacgc aggtgccgat cgggtcgatg cgcttgtcgt tggtgaagac
      841 cgcgatcttg gctcggatgc cggggtcgcg cgccgccgac ttgatctcga gcagcccctc
      901 ctcgatctcc ggcacttcga gctcgaacag cttcatgatg aactgtggcg cggtgcgcga
      961 caggatgagc tgcggcccgc gcgcctggcg gtcgatcttc atcacccagg cgcgcacccg
     1021 gtcgcccacc cggaggttct ccttcgggat catgtgctcg cgcggcagaa gcgcttccag
     1081 gcggcccgac tcgatgatcg cgttgccgcg ctccatgcgc ttcacggttc cggtcaccag
     1141 ctcgtcgccg cgcgacagaa agtcgttcag gatctgctcg cgctcggcat cgcggatgcg
     1201 ctgcaggatc acctgcttgg cggtctgcgc gccgatgcgg ccgaactcga tcggctcgag
     1261 cggctcctcg atgaagtcct cgagcttgac gtcggccttg cgcttctgcg cctcgctgag
     1321 cgcgatctgc gccggcggcg actcgatcgc ctcgtcgggc acgaccttcc agcggcggaa
     1381 cgactcgtac gcgccggtgt tgcggtcgac ctgcacgcgc acgtcgatgt cctcggagta
     1441 ttttttcttg gtcgccgaag cgagcgccat ttccagcgcg ccgaagacga tgtcccgcgg
     1501 cacgttcttc tcccgcgcga gcacgtccac cagcatcacc agttcacgat ttcctgccgc
     1561 tatcgcattc gctgccgcca cgagatcctc acaattcagg tatcagcttc gcccgatcga
     1621 cgtcttcgag cgccagccgc actgtctgtc cttccagctc catgttcacc agccccgcct
     1681 cggcgccgcg cagcacgccg acaaacctgc gccggccggt cgcgtcgggc gttttcatcc
     1741 gcacctcggc tttgtgcccc gagaaacggg cgaagtcgcc cgccttgcgc aggcgacggt
     1801 ccaggcccgg cgaggaaacc tccaggcgct catagtcgat tccctcgacc tccagcaccc
     1861 gcgtgagctg gcggcttacc gccgcgcagt cctcgaggcc cacgccgccg ggcttgtcga
     1921 taaacaggcg cagcagccgc ccgccgttcg acgcttgcgc gtcgacgagc tcgaagccca
     1981 tcccggccac caccggctcc actaccgaat cgatcccggc catttgcggc tttcccggcg
     2041 ccgcaaacaa aaaatgggcc gaagcccatc tttgcgcggt gccactaaag cgtttgaagt
     2101 ttaacagtat ttcagtgccg caacaacctc tgcaggggtt gcgcagcgag ctcgcgccag
     2161 cgccccgccg gcaggtcgcg gggtagctcc accggtccgt agcgaacgcg catcaggcgg
     2221 ctgacgcgcg cgccgacagc ttcgaacagc cgccgcactt cgcgattgcg cccttcccgc
     2281 agcaccaccc ggtaccagcg gttcgctcct tcggcgcctg cgtttttttc catgaattca
     2341 atgcaagtaa acgccgccag gccatcctcg agctggacgc caagtttcag cttttcaagc
     2401 gcttcaggcc cgaggcgccc ctgaacgcgg gccgcgtact cgcgctcgat ctcgtagcgc
     2461 ggatgcatca gacggttggc aagctcgccg gaattggtga gcagcagcag tccggagctg
     2521 ttgatgtcga gccggccgac ggctacccag cgccccgcgg gcaaacgcga gaacaccgtc
     2581 gggcgccctt cggggtcgct gcgggtgacg agctcgccga ctcgcttgtg atagaggagc
     2641 actcgcggcg ccgattttgc cggcaaccac gccacggggt tgccatcgac ggcaatttcg
     2701 tcgtcctcga cagcg
//
LOCUS       DASPXW010000193         3390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_936488, whole genome shotgun sequence.
ACCESSION   DASPXW010000193 DASPXW010000000
VERSION     DASPXW010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936488"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="VEL04_04590"
     CDS             <1..887
                     /locus_tag="VEL04_04590"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYT47673.1"
                     /translation="PVRMSIPAAPGGNPDVLARMLAQKLAEAFGRPFVVENMPGAGGV
                     VAAEAVVKTAADGHVLFLGDSGALAINVAVNPNISFHPLRDFTLITALVSVPTVLVVN
                     PAVPAASLQEFVALAKSKPGQLAFGSAGNGSIHHLTMAIFASRAGIDLLHVPYKGGTA
                     LVAAVLSGEVQSGWSGIPNVLQAIKAGRLRVLCISTAKRSASLPDAPTAAELGYPGFN
                     VATTIGLQAAAGVPAEIVAGLQAGFARALREPDIAARMANLGMELAENGTESYVRYVR
                     EDLERYAAAVKVLGGKVQ"
     gene            884..2029
                     /locus_tag="VEL04_04595"
     CDS             884..2029
                     /locus_tag="VEL04_04595"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HYT47674.1"
                     /translation="MSGLARLHTDVVGSLLRPAAWKEARLRLDSGLLDKNSFNEIELE
                     CVRRHVALQESVGLDVVTDGEVSRLNFQDSFGLSVGGYDTAAESVASHERRAAGGTPL
                     SRWEIPDLAGPGTPVIHRRPVTSRLRLTRNVPLEEYVRVAPLARKPVKVTLIGPDRIA
                     QRFDHARSKDIYRTMDEFVADVVSIQRAMVRQLVDAGCRYVQIDAPGYTAYVDEPSLA
                     AMRARGEDPMENFSRSLKADAAVAEGFPGVTFGIHLCRGNQRSMWHREGTYDAIAERL
                     FNELPHARFLLEYDSPRAGSFAPLRFVPKGKVVVLGLISTKVPQLEPVEELKRRIGAA
                     AKYVPLENLAISPQCGFGSDVVGNLVSEDHQKRKLERVVETARQVWR"
     gene            2026..3063
                     /locus_tag="VEL04_04600"
     CDS             2026..3063
                     /locus_tag="VEL04_04600"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYT47675.1"
                     /translation="MTDLQCGIFDWVEASGRSPGAVYAHKLELAAAADQAGLHAYLIA
                     EHQGTPLSIDGSPSVLLSAMIQRTRRLRVGALTFCLPWYNAYRFYNEVCMLDQLSGGR
                     LQLGVGRGVSPIESLIFDMKGVEESRERYRETLEVFFAACKNRVLNFQGRYYNYRDVE
                     LYNKPLQQPYPPLWFPSSNRESIEFTARHGYHTALLAKLADCKPLFEQYREIWERHRN
                     DPGRHNAHVAAPFLAKTQHLVIADTEAEAERLGLEAYGKWAARIHHLTRKLGRPDVHK
                     TEPYAEDSAQRLVTGTPRTALEKLQEVIRVTGANYLLCIFSFGDIAPEHALRSLELFS
                     REVMPKLEGRN"
     gene            3060..>3390
                     /locus_tag="VEL04_04605"
     CDS             3060..>3390
                     /locus_tag="VEL04_04605"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYT47676.1"
                     /translation="MKPDQDQAILDAIDRWLRRDVEPRVAQLERDDVWPAEMVEQMKT
                     LGLFGATISPRYGGLGLPAQLYARIVARISEVWMSLTGIFNSHLMMALLVEKNGSSFL
                     KEKYLPKF"
BASE COUNT          584 a         1134 c         1121 g          551 t
ORIGIN      
        1 ggccggtccg catgagcatt cccgcggcgc cgggcgggaa tcccgatgtg ctcgctcgca
       61 tgctggcaca gaaactcgcg gaggctttcg gccggccttt cgtcgtcgag aacatgcccg
      121 gcgccggcgg cgtggtcgct gcggaagccg tggtcaagac tgcggccgac gggcacgtgc
      181 tgttcctggg cgattcaggc gccctcgcga tcaacgtggc agtgaatccg aacatcagct
      241 tccacccgtt gcgcgatttc accctgatca cggcgctggt gtcggtgccg acggtgctcg
      301 tcgtcaaccc cgccgtgccc gccgcgtcgc tgcaggagtt tgtcgcgctc gccaaatcga
      361 agccgggaca gctggcgttc ggctcggccg gcaacggctc gatccaccat ctcaccatgg
      421 caatcttcgc ctcgcgcgcc gggatcgacc tgctgcatgt cccctacaag ggcgggacgg
      481 cgctggtcgc cgccgtgctc tcgggagaag tgcagtcggg atggtcgggc atcccgaacg
      541 tgctgcaggc gatcaaggcg ggccggttgc gcgtgctgtg catcagcacg gcgaaacgct
      601 cggcttcgct gccggatgcg ccgaccgccg ccgagctcgg ctatcccggg ttcaacgtgg
      661 cgaccaccat cggcctgcag gccgctgccg gcgtgccggc cgaaatagtc gccgggctgc
      721 aggccggttt cgccagggcg ctgcgcgagc ccgacatcgc cgcacgcatg gcgaatctcg
      781 gcatggagct cgccgaaaac ggcaccgaga gctacgtgcg atatgtgagg gaagatctgg
      841 agcgctacgc cgccgcagtc aaggtgctgg gaggcaaggt gcaatgagcg gcctggcaag
      901 gctgcacacc gacgtcgtcg gcagcctgct gcgcccggcg gcatggaagg aggcgcgcct
      961 gcggctcgac agcgggctgc tcgataaaaa ttccttcaat gaaatcgaac tggaatgcgt
     1021 tcgacgccac gtggcgttgc aggaaagcgt cgggctcgat gtcgtcaccg acggcgaggt
     1081 cagccggctc aatttccagg atagcttcgg gctgtcggtg ggcggctacg ataccgcggc
     1141 cgaaagcgtc gcatcgcacg aacggcgcgc cgccggcggc actccgctca gccgctggga
     1201 gattccggac ctcgccgggc ccggcacgcc ggtaatccat cgccggccgg tgacgagccg
     1261 cctgcggctc acgcgcaacg ttccgctgga ggaatatgtg cgcgtcgcgc cgctcgcccg
     1321 gaaaccggtc aaggtgacgc tgatcggccc cgatcgcatc gcgcagcgct tcgaccacgc
     1381 gcgctcgaag gacatctacc ggaccatgga tgagttcgtc gccgacgtgg tctcgatcca
     1441 gagggcgatg gtgaggcagc tggtcgacgc cggctgccgc tacgtgcaga tcgacgcgcc
     1501 gggatacacc gcgtatgtcg acgagccgtc gctcgccgcg atgcgcgcgc gcggcgaaga
     1561 cccgatggaa aacttttcgc gctcgctgaa agccgacgcg gccgtggccg aaggctttcc
     1621 cggcgtgacc ttcgggattc atctctgtcg cggcaaccag cgcagcatgt ggcaccgcga
     1681 ggggacttac gatgcgatcg ctgagcgcct cttcaacgag ctgccgcacg cacgcttcct
     1741 cctcgagtac gacagcccgc gcgccggcag cttcgcgccg ctgcgcttcg tgccgaaagg
     1801 caaggtggtg gtgctcgggc tcatcagcac caaggtgccg cagctcgagc ctgtcgagga
     1861 gctcaagcgc cgtatcggcg ccgcggcgaa atacgttccg ctcgaaaacc tggccatcag
     1921 cccgcaatgc ggcttcggct cggatgtcgt cggcaacctc gtcagcgagg accaccagaa
     1981 gcgcaagctc gagcgggtgg tggaaaccgc gcggcaggta tggcgatgac ggatctgcaa
     2041 tgcggcattt tcgactgggt cgaggcgtcc gggcgctcgc cgggagcggt ctacgcgcac
     2101 aagctcgagc tcgccgccgc cgccgaccag gccggcttgc acgcctatct catcgccgag
     2161 caccagggca cgccgctctc gatcgacggc tcgccgagcg tgctgctgtc ggcgatgatc
     2221 cagcgcacac ggcggctgcg ggtaggggcg ctcaccttct gcctgccctg gtacaacgcc
     2281 taccgcttct acaacgaggt ctgcatgctc gaccagctct cgggcgggcg cctccagctc
     2341 ggcgtcggac gcggcgtgtc gccgatcgaa tcgctgatct tcgacatgaa aggcgtcgag
     2401 gaatcgcgcg agcgctaccg tgaaacgctc gaggtatttt ttgccgcgtg caaaaacagg
     2461 gtactcaatt tccagggcag gtactacaac taccgcgacg tcgagctgta caacaagccg
     2521 ctgcagcagc cgtatccgcc gctctggttc cccagcagca accgcgagtc gatcgagttc
     2581 accgcgcgtc acggctatca caccgcgctg cttgcgaagc tcgccgactg caagccgctc
     2641 ttcgaacagt accgcgagat ctgggagcgc caccggaacg atcccggccg ccacaatgcg
     2701 catgtcgcgg cgccctttct cgcgaagacg cagcacctgg tgatcgccga cacggaggcc
     2761 gaggccgaga gacttggatt ggaggcgtac ggcaagtggg ccgctcgcat ccaccacctc
     2821 acgcgcaagc tcggccgccc cgacgtgcac aagaccgaac cctacgccga ggattccgcg
     2881 cagcgcctgg tcaccggcac accgcgtacg gcgctcgaaa agctgcagga agtgatccgg
     2941 gtgaccgggg cgaactacct gctgtgcatc ttctcgttcg gcgacatcgc gccggagcac
     3001 gcgctgcgct cgttggagct cttctcccgg gaagtgatgc ccaagctgga gggccgcaat
     3061 tgaagcccga ccaggaccag gcgatcctcg acgcgatcga ccgctggctg cggcgcgacg
     3121 tcgagccgcg cgtggcgcag ctcgagcgcg acgatgtctg gccggcggag atggtcgagc
     3181 agatgaagac tctcggcctc ttcggcgcga cgatcagccc gcgctacggc gggctcgggc
     3241 tcccggcgca gctctacgcg cgcatcgtcg cgcgcatctc ggaagtctgg atgtcgctca
     3301 ccgggatctt caattcccac ctcatgatgg cgcttctcgt ggaaaaaaat ggttcttcat
     3361 ttttgaaaga aaaatatttg ccgaaattcg
//
LOCUS       DASPXW010000194         3222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1319660, whole genome shotgun sequence.
ACCESSION   DASPXW010000194 DASPXW010000000
VERSION     DASPXW010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1319660"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VEL04_04610"
     CDS             <1..317
                     /locus_tag="VEL04_04610"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYT47677.1"
                     /translation="SERKVVAMMSSRLGSISEMRGETLPYATSKAALNALAKGLAVTL
                     ASRGIIVVALSPGWVRTGMGGKGAPLAPETSVRGLRKVIPGLKPSDSGKFLSYDGSTI
                     QW"
     gene            328..1200
                     /locus_tag="VEL04_04615"
     CDS             328..1200
                     /locus_tag="VEL04_04615"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYT47678.1"
                     /translation="MLHADSARNRLIGIGLVSFTYLLFALLDGSAKWLVGSVPVIVVV
                     WLRFVIHVAAAGAVLFPLRGLSLIKTSHLRWHLLRAVMFIAMTGINFWALQYLQLTVT
                     SSIFFTVPLIIAVLGAPLLGEQLDRGRWAAILVGFAGVLVIIRPGSAEFHPAMLASVV
                     NALLYACFMLMTRRLAAYDSPETIQYLPAVGAAIGLAPFALAAWESPEGWLEWSVACV
                     MGVLGGLGHYLLALAHRYAPATVIAPFLYQQVFYMALFGYLVFGDVPGAAVWTGAGIV
                     IASGLYLFYRERTA"
     gene            1197..2597
                     /locus_tag="VEL04_04620"
     CDS             1197..2597
                     /locus_tag="VEL04_04620"
                     /inference="COORDINATES: protein motif:HMM:NF039580.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HYT47679.1"
                     /translation="MNDPVALPFWVTAILFVFAAWALYEHVLMPALRWMVTHPANQVI
                     DELSTRLRIGIRPFQRTRRQALIHRLLADPKVQQAAEHFSTEKKISLEAALKQVERCA
                     REIVPAFNAYLYFRIGYWIGRSIARALYRVRLGYIDSAGIARIDADATVVLVMNHRSN
                     MDYILAAFLAADQAALSYAVGEWARIWPLSALIRAMGAYFVRRNSGDELYRRVLERYI
                     AMATEAGVPQAVFPEGGLSRDGLMREPRLGVLDYTMRGFHIDGARDLVFVPLGINYDR
                     VLEDRTLLRTLEGKKAGRLRAISGTFGFVLHNLLLVLRSEWRRFGYACVNFGSPISMR
                     EYCRAQQIDFQKLSGDPRRAAMARLGRELMQAVGRVVPVVPVALMATVFGSNPERALS
                     ELELKSEVGRLLQRLEAGGARVYVPRGDLDYALTVGLRMLRLRHMVEEKDGLYAAAAA
                     ELPLLGYYANSIAHLL"
     gene            2659..>3222
                     /locus_tag="VEL04_04625"
     CDS             2659..>3222
                     /locus_tag="VEL04_04625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HYT47680.1"
                     /translation="MARRSSRSATIKRSGARRRDFAGGRTVFHDLPSDWYWEQDAELR
                     FSKVEVRNGAPAEQALAARILGKQRWETGIDIEGGWDAHRAALEARQPFRDVLMWRIF
                     EDGSRRYLSVTGEPVFDAKGRFTGYRGVGRDVTAQKRVERLLRLEHRVTRCLAEAQSA
                     PAALGTALKAICETEGWDCAEFWKLDES"
BASE COUNT          481 a         1059 c         1103 g          579 t
ORIGIN      
        1 cgagcgagcg caaggtggtg gcgatgatgt cgagccgcct cggctcgatc tccgagatgc
       61 ggggcgagac cctgccctac gcgaccagca aggcggcgct gaacgcgctc gccaaggggc
      121 tcgcggtcac gctcgcgagc cgcggcatca tcgtggtcgc cctcagcccg ggctgggtgc
      181 gcaccggcat gggcggcaaa ggcgcgccgc tcgcgcccga gacctcggtg cgcgggctgc
      241 gcaaggtcat ccccggcctg aagccgtccg actccggcaa attcctgtcg tacgacggct
      301 cgacgatcca atggtaagag caagaccttg ctccacgccg acagcgcccg caaccgcctg
      361 atcggcatcg ggctggtctc gtttacctat ctgctgttcg cgctgctcga cggcagcgcg
      421 aagtggctgg tggggagtgt gccggtaatc gtcgtggtat ggctgcgctt cgtaatccac
      481 gtcgccgccg ccggggcggt cctgttcccg ctcagaggtc tgtcactgat aaagacaagc
      541 cacctgcgct ggcatctgct gcgcgcggtc atgttcattg ccatgaccgg catcaacttc
      601 tgggcgctgc aatatctgca gctcacggtg acctcgtcga tcttcttcac ggtgccgctt
      661 atcattgcgg tcctgggcgc gccgctcctc ggcgagcagc tcgaccgcgg ccgctgggcc
      721 gcgatcctgg tcggcttcgc cggggtgctg gtgatcatcc gtcccggctc ggccgagttc
      781 catcccgcga tgctcgcttc ggtggtcaac gcgctgctgt acgcgtgctt catgctgatg
      841 acgcggcgtc tcgcggccta cgattctccc gagaccatcc agtatctgcc cgcggtaggc
      901 gcggcgatcg gacttgcgcc cttcgcgctc gcggcatggg agtcgcccga gggttggctg
      961 gaatggagcg tcgcctgcgt gatgggcgtg ctgggcgggc tcggccatta cctgctcgcg
     1021 ctcgcgcacc gctacgcgcc ggcgacggtg attgcgccat tcctgtatca gcaggtgttc
     1081 tatatggcgc tcttcggcta cctcgtcttc ggcgacgtcc ctggcgccgc ggtatggacc
     1141 ggcgcgggga tcgtgatcgc cagcgggctg tatctgttct accgggaaag aacggcgtga
     1201 acgacccggt ggcgctgcca ttctgggtaa cagcaattct ttttgtgttt gccgcgtggg
     1261 cactgtacga gcatgtactc atgccggcgc tgcgctggat ggtgacccat cccgcgaacc
     1321 aggtgatcga cgagctctcg acgcggctgc gcatcggcat ccggccgttc cagcgcacgc
     1381 gccgccaggc gctgatccat cgcctgctgg ccgatcccaa ggtgcagcag gcggcggagc
     1441 atttttccac cgaaaagaaa atttccctcg aagcggcgtt gaagcaggtc gagcgctgcg
     1501 cgcgcgagat tgtgccggcg ttcaacgctt atctgtattt ccgcatcggc tactggatcg
     1561 gccgcagcat cgcgcgcgcg ctgtatcgcg tgcgtctggg ctacatcgac tctgccggca
     1621 tcgccaggat cgacgccgac gcgacggtgg tgctggtcat gaaccaccgc tccaacatgg
     1681 attacatcct tgccgcgttc ctcgccgccg accaggccgc gctgtcgtac gcggtcggcg
     1741 aatgggcgcg catctggccg ctctctgcgc tgatccgcgc catgggcgca tatttcgtgc
     1801 gtcgcaactc gggcgacgag ctctaccggc gggtgctcga gcgctatatc gcgatggcga
     1861 ccgaggccgg cgtgccgcag gcagtgttcc ccgagggcgg cctgtcgcgc gacggcctca
     1921 tgcgcgagcc gcgcctcggc gtgctcgact acacgatgcg cggcttccac atcgacggcg
     1981 cgcgcgacct ggtattcgtg ccgctcggca tcaactacga cagggtgctg gaggatcgaa
     2041 cgctgctgcg caccctcgag ggcaagaaag caggccggct gcgagccatc tcgggcacgt
     2101 tcggcttcgt gctgcacaac ctgctgctcg tgctgcgcag cgaatggcgc cgcttcggct
     2161 atgcctgcgt gaatttcggc tcgccgatct cgatgcgcga gtactgccgc gcgcagcaga
     2221 ttgatttcca gaagctttcg ggagatccgc gcagggcggc gatggcgcgc ctgggccgcg
     2281 agctgatgca agcggtggga cgcgtcgtgc ccgtggtgcc ggtggcgctg atggcgacgg
     2341 ttttcggaag caatcccgag cgcgccctct ccgagctcga gttgaagtcg gaggtgggac
     2401 gcctcctgca gcgcctcgaa gccggcggcg cgcgtgtcta cgtgccgcgc ggcgacctcg
     2461 attacgcgct caccgtgggg ctgaggatgc tgcgcctgcg gcacatggtg gaggagaagg
     2521 acggcctgta cgccgcggcc gcggcggagc tgccgctgct cggctattac gccaactcca
     2581 tcgcgcacct gctctgattt gactggactc tgagataagc ctccagtaaa ttcgccgcaa
     2641 gggagggaaa attcggtaat ggcgaggcgc agcagccgct cggccacgat caagcgcagc
     2701 ggtgcgcgcc ggcgcgactt tgccggcggc cgcacggtgt ttcacgacct gccctcggat
     2761 tggtactggg agcaggacgc ggagctgcgt ttctcgaagg tcgaggtgcg caacggcgcg
     2821 cctgccgagc aggcgctcgc ggcccgcatc ctcggcaagc agcgctggga aaccggcatc
     2881 gacatcgaag gcggctggga cgcgcaccgc gcggcgctcg aggcgcgcca gccgttccgc
     2941 gacgtgctga tgtggcgcat cttcgaggac ggcagccggc gctacctcag cgtcaccggc
     3001 gagccggtgt ttgacgccaa agggcgcttc accggctacc gcggtgtcgg acgcgatgtc
     3061 actgcgcaga agcgcgtgga gcggctgctc cgcctggagc accgggtgac gcgctgcctc
     3121 gcggaagcgc aaagcgcccc cgctgcgcta ggcacggcgc taaaggcgat ctgcgaaacc
     3181 gaaggctggg actgcgccga attctggaag ctcgacgaat cc
//
LOCUS       DASPXW010000195         2670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_307464, whole genome shotgun sequence.
ACCESSION   DASPXW010000195 DASPXW010000000
VERSION     DASPXW010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307464"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VEL04_04630"
     CDS             complement(<1..227)
                     /locus_tag="VEL04_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003275847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47681.1"
                     /translation="MKQSLHMLARDWRSGELRVLALALVIAVASVSSVAFLGDRVSRA
                     IVRDAHQLLGADLVLLSDHPWNEAFIEKLTS"
     gene            complement(224..877)
                     /locus_tag="VEL04_04635"
     CDS             complement(224..877)
                     /locus_tag="VEL04_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT47682.1"
                     /translation="MARGIGKTVKSGDSDLVILRDIDLEVTRAEAVAVVGASGSGKST
                     LLAILAGLDTPTSGSVELDGADLFSMNEDQRAELRGRSLGFVFQSFQLLPALTALENV
                     MLPLELSNHPDPEKDSKEVLARVGLGERLHHYPKHLSGGEQQRVALARAFVVRPKVLL
                     ADEPTGSLDPESGAGVIQLLFEMNREYGTTLVMVTHDETLAARCARIVRLVAGRIAA"
     gene            897..1481
                     /locus_tag="VEL04_04640"
     CDS             897..1481
                     /locus_tag="VEL04_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HYT47683.1"
                     /translation="MLVVAAPAAPAADKSIVVLGDSVSAAFGIPQARGWVALLGERLK
                     RERLDYSVVNASISGETTGGGAARLRAALDRHRPAVVIIELGGNDGLRGLPVAQMKKN
                     LAAMIEQSQKAGARVLLVGMRVPPNYGPEYGGEFASAFGELAKRYRVALVPFPFEGFD
                     EKPGLFQPDRIHPTEAAQPLMLEQIWKKLRPLLK"
     gene            complement(1482..2531)
                     /gene="selD"
                     /locus_tag="VEL04_04645"
     CDS             complement(1482..2531)
                     /gene="selD"
                     /locus_tag="VEL04_04645"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HYT47684.1"
                     /translation="MNAPEKIRLTEFSHGGGCGCKIAPAVLSEILAASPIRGLPKELL
                     VGTETADDAAVYLLNPGQALVATTDFFSPVVDDPYDFGRIAATNAISDIYAMGAKPIL
                     ALALVGMPLDKLPVSVIRSILAGGESVCAEAGIPIAGGHSIDTLEPIYGLVALGLVHP
                     DKVKRNSSAKPGDLLILGKPLGIGILSAALKKGKLSAAGYAQMIDWTTKLNTPGEALA
                     EMKEVHAVTDVTGFGLAGHLLEMLRGAKLSAEVRFDALPVIAEALEWAKQGVATGASE
                     RNWKGYGEEVSLPVDFPGWKSKLLTDPQTSGGLLVACAPGAEKKVLQEFRKRGFGEAC
                     VIGKLSAGAARLTVS"
     gene            2549..>2670
                     /locus_tag="VEL04_04650"
     CDS             2549..>2670
                     /locus_tag="VEL04_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HYT47685.1"
                     /translation="MNFSEFDSVIDARTPSEYAADHVPGAISAPVLDDAERATVG"
BASE COUNT          445 a          905 c          879 g          441 t
ORIGIN      
        1 tcggaagtca gtttttcaat gaatgcttca ttccatgggt ggtcggagag cagcaccaag
       61 tcggcgccga gcagctggtg cgcgtcgcgc acgatggcgc ggctcacgcg gtctcccagg
      121 aaggcgacgc tcgaaacgct cgctaccgcg atgaccagcg ccagggcgag cacgcgcagc
      181 tcccccgagc gccagtcgcg cgcgagcatg tgcagcgact gcttcatgcc gcgatccgcc
      241 cggcgaccag ccgcacgatc cgcgcgcagc gcgcagcgag cgtctcgtcg tgcgtcacca
      301 tgacgagggt ggtgccgtat tcgcggttca tctcgaagag cagctggatc accccggcac
      361 ccgactccgg gtcgaggctg ccggtcggct cgtcggcgag cagcactttc ggccgcacca
      421 cgaaggcgcg cgcgagcgcg acgcgctgct gctcgccgcc cgagaggtgc ttcggatagt
      481 ggtgcaggcg ctcgcccagg ccgacgcgcg ccagcacttc tttcgagtct ttctccggat
      541 ccgggtgatt cgagagctcg aggggaagca tcacgttctc gagcgcagtc agcgcgggca
      601 gcagctggaa cgactggaac acgaagccga gcgagcgccc gcgcagctcg gcccgctggt
      661 cttcgttcat cgagaagagg tccgcgccgt cgagctcgac gctgccgctg gtcggcgtat
      721 cgaggccggc caggatggcg agcagcgtcg acttgcccga gcccgaggcg ccgaccaccg
      781 cgaccgcctc ggcgcgcgtg acctcgagat cgatgtcgcg cagaatgacg aggtcggaat
      841 ccccgctctt cacggtcttg ccgatgccgc gcgccatcag gacattttta ttctgcatgc
      901 tggttgtggc ggcgcccgcg gcgccggcgg cggataaatc cattgtggtg ctcggtgaca
      961 gcgtgtcggc ggccttcggc atcccccagg cgcgcggctg ggtggcgctg ctcggcgagc
     1021 gcctgaagcg ggaacgcctc gattatagcg tcgtcaacgc cagcatctcc ggcgagacca
     1081 cgggcggcgg ggcggcgcgc ctgcgcgcgg cgctcgacag gcaccgcccg gcggtggtga
     1141 tcatcgagct cggcggcaac gacggcctgc gcggcctgcc ggtcgcgcag atgaaaaaaa
     1201 atctcgccgc gatgatcgag cagtcgcaga aggcgggcgc gcgcgtgctg ctcgtgggca
     1261 tgagggtgcc gcccaactac gggccggaat acggcggcga gttcgcatcg gctttcggcg
     1321 agctggcgaa gcgctatcgc gtcgcgctgg tgccgttccc gttcgaaggt tttgacgaga
     1381 aacctggact gttccagccc gaccgcatcc accccaccga ggcggcgcag ccgctgatgc
     1441 tcgagcagat ctggaagaag ctccggccct tgcttaagta atcagctcac cgtcagccgg
     1501 gcagcgccgg ccgagagctt gccgatcacg caggcctcgc cgaagccgcg cttcctgaac
     1561 tcctgcagca ctttcttctc cgctccgggc gcgcaagcaa ccagcaggcc gccgctcgtc
     1621 tgcgggtcgg tgagcagctt gcttttccaa ccaggaaaat ctaccggaag agacacctct
     1681 tcgccgtaac ccttccagtt gcgctctgaa gcgccggtcg ccacgccctg cttcgcccac
     1741 tcgagcgctt cggcaatgac cggcagcgca tcgaatctca cttcggccga gagcttcgcg
     1801 ccgcgcagca tctcgagcag gtgcccggcg agcccgaagc ccgtcacatc ggtaaccgca
     1861 tgcacctcct tcatctccgc gagcgcttcg ccgggcgtgt tgagcttggt cgtccagtcg
     1921 atcatctgcg cgtagcccgc cgcggacagc ttgcctttct tcagcgccgc cgaaaggatg
     1981 ccgatcccca ggggttttcc caaaatgagc aaatcacctg gcttcgccga gctattccgc
     2041 ttcaccttgt ccgggtggac gagacccaag gccaccaggc cgtagatcgg ctccagcgta
     2101 tcgatcgaat gcccgccggc gatcggaatg ccggcttcgg cgcacaccga ctcgccgccg
     2161 gcgaggatgc tgcggatcac cgacaccggc agcttgtcga gcggcatgcc gaccagcgcg
     2221 agcgcgagga tcggtttcgc gcccatggca tagatgtcgg agatggcgtt ggtcgcggcg
     2281 atgcggccga agtcgtacgg atcgtcgacc accggcgaga agaagtccgt ggtcgcgacc
     2341 agcgcctgcc cggggttgag gagatatacc gcggcgtcgt cggcggtctc cgtgccgacg
     2401 agcagctctt tcggcagccc tcttatcgga gaagcagcca ggatctcaga cagcaccgcc
     2461 ggcgcgatct tgcagccgca gccgccgccg tggctgaact cggtcagccg aatcttttcg
     2521 ggagcattca tgagcgtaga atttaaccat gaatttcagc gagttcgata gcgttatcga
     2581 cgcccgcacg ccgtcggaat acgccgcaga ccacgttccc ggcgcgattt cggcgccggt
     2641 gctcgacgat gccgagcgcg ccacggtcgg
//
LOCUS       DASPXW010000196         2771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1820552, whole genome shotgun sequence.
ACCESSION   DASPXW010000196 DASPXW010000000
VERSION     DASPXW010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1820552"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="VEL04_04655"
     CDS             <1..809
                     /locus_tag="VEL04_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYT47686.1"
                     /translation="FLLSAGFSLIFGLMRIPNLLHGSFFMLGAYFGVSLLAKGYNFWL
                     AALLSGAAMAVIGAFIERFLMRRLAGQELAQVLLTLGLSFIVADLCLMVWTGDPWQPS
                     TPSPFTGPLQVAGLFFPIYRLVIIVVAVLVAVALWLLVDWTRLGAMIRAGVDDAPIAR
                     VVGIKVSQLFTLIFALGAGLAAFAGVIGSPVLSVYPGLDFEMLPLALIVVILGGTGSL
                     LGALVGSFIIGFLYNFGQAMFPDLAYVILFLPMLLVLVLRPQGLFGKVVP"
     gene            806..1804
                     /locus_tag="VEL04_04660"
     CDS             806..1804
                     /locus_tag="VEL04_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYT47687.1"
                     /translation="MTAVTPRRAALAIAAVALVTLPIWVGNPYYINVASQILLFAVFA
                     LALNVLVGYGGLVSLGHAGLFAMAGYAGALILQAGHGHFLADAGAIVVTVAASAVFGL
                     LALRSTGIGFLMITLALGQIVWGIAYRWASLTNGDNGVNVSSRPAPFGISLANASSFY
                     LATLLVFVLAVATMALFVRSPFGASLRGTRDQARRMNALGYNVWLIRFIAFLFSGFWT
                     GVAGLLFIYYNQFISPQVSALTASAEVLLMVISGGTATLLGPIVGAAIVVIMKNVVSA
                     YVIRWNMVLGLIFVLIITFMPEGLVPGSVRLWKMGRQAWRGRNAVAPPAKLGERAP"
     gene            1801..2538
                     /locus_tag="VEL04_04665"
     CDS             1801..2538
                     /locus_tag="VEL04_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT47688.1"
                     /translation="MTALAVKGLDKSFGGLRVTANVTFDVQPGERRLIIGPNGAGKTT
                     LFNLITGEIAPDSGSIILNGQDITRVPSRRRPHLGMARTYQIITLFSRDTILRNVTLA
                     LLGLSKLRWDPFIDLDRQHHLVEAAHAALTRVGLDHIAERPLAQTSYGERRRVEIAMA
                     LAQNPKVLLLDEPFAGLSIDERRDVQQLLLAIPRDVTIVMIEHNMDVALEFAERITLL
                     HFGEVIVEGSRAEVVADPRTREVYLGH"
     gene            2528..>2771
                     /locus_tag="VEL04_04670"
     CDS             2528..>2771
                     /locus_tag="VEL04_04670"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYT47689.1"
                     /translation="MATSALRLSDVDAFYGDSHVLEKVSFAAGEGRLLGLLGRNGAGK
                     STCMNVTVGLLPARRGSVEVFGVPVTRLPPEQIAAQG"
BASE COUNT          358 a          947 c          918 g          548 t
ORIGIN      
        1 tgttcctgct gtcggcgggc ttctcgctca tctttggcct gatgcgcatc cccaacctgc
       61 tgcacggctc gttcttcatg ttgggggcct atttcggcgt cagccttctc gccaagggct
      121 ataacttctg gctcgccgcc ctgctcagcg gggcggcgat ggccgtgatc ggggcgttca
      181 tcgagcgctt cctcatgcgc cggctggcgg ggcaggaact cgcgcaagtg ctgctcaccc
      241 tcggcctctc cttcatcgtc gccgatctct gcctgatggt gtggaccggc gatccctggc
      301 agccgtcgac accaagcccc ttcacgggcc ctctgcaggt cgccggcctt ttctttccga
      361 tttatcgcct tgtgatcatc gtcgtggcgg tattggttgc cgtcgcgctc tggctcctgg
      421 tcgattggac gcggctcggc gcgatgatcc gcgccggcgt cgacgacgcg ccgatcgcgc
      481 gcgtggtcgg catcaaggtg tcgcagctct tcacgctgat cttcgcgctc ggtgcagggt
      541 tggcggcctt cgccggcgtg atagggtcac ccgtcctgtc ggtttatccg ggactcgact
      601 tcgaaatgct gccgctcgcg ctgatcgtgg tcatcctggg cggcaccggc agcctgctcg
      661 gcgcgctggt cggcagcttc atcatcgggt ttctctacaa tttcgggcag gcgatgtttc
      721 ccgacctcgc ctacgtgatc ctgttcctgc cgatgctgct cgtcctggtg ctgcggccgc
      781 agggcctgtt cggcaaggtc gtgccatgac ggccgtcacg ccgcgccgcg cggccctcgc
      841 cattgccgcc gtcgcgctgg tcacgctgcc gatctgggtg ggcaatccct attacatcaa
      901 tgtggcgagc cagatcctgc tgttcgcggt gttcgcgctc gcgctcaacg tgctggtcgg
      961 ctacggcggc ctggtctcgc tcggccatgc cgggctgttc gccatggccg gctatgccgg
     1021 cgcattgatc ctgcaggccg gccatgggca tttcctcgcc gacgccggcg cgatcgtcgt
     1081 cacggtcgcc gcctccgcgg tgttcggcct gctcgcgctg cgctcgaccg gcatcggctt
     1141 cctgatgatc acgctcgcgc tcgggcagat cgtgtggggc atcgcctatc gctgggcgag
     1201 cctcaccaac ggcgacaacg gcgtgaacgt atcgagccgg ccggcgccgt tcggcatcag
     1261 cctcgccaac gccagctcgt tctatctcgc gacgctcctc gtgttcgttc tcgccgtcgc
     1321 gaccatggcg ctgttcgtgc gctcgccgtt cggcgcgagc ctgcgcggca cgcgcgacca
     1381 ggcgcgccgc atgaacgcgc tcggctacaa tgtctggctc atccgcttca tcgccttcct
     1441 gttctcgggc ttctggaccg gcgtcgcggg cctgctgttc atctactaca accagttcat
     1501 cagcccgcag gtgtccgcct tgacggcctc ggccgaggtg ctgctgatgg tgatctccgg
     1561 cggcaccgcg acgctgctcg gcccgatcgt gggcgcggca atcgtggtca tcatgaagaa
     1621 cgtggtgagc gcctatgtga tccgctggaa catggtgctg ggattgattt tcgtcctcat
     1681 catcacgttc atgccggaag gcctggtgcc gggctcggtg cggctgtgga agatgggccg
     1741 gcaggcctgg cgcggacgaa acgccgtcgc gccgcccgcg aagctcgggg agcgcgcgcc
     1801 atgaccgcgc tcgccgtcaa agggctcgac aagtcgttcg gcggattgcg cgtgaccgcc
     1861 aatgtcacgt tcgacgtcca gccgggcgag cgccggctga tcatcggccc caacggcgcc
     1921 ggcaagacca cgctgttcaa cctgatcacc ggcgagatcg ccccggactc aggatcgatc
     1981 atcctcaacg ggcaggacat cacgcgcgta ccgagccggc ggcggccgca tctcgggatg
     2041 gcgcgcacct accagatcat cacgctgttc tcgcgcgaca ccatcctgcg caacgtcacg
     2101 ctcgcgctgc tcgggctgtc gaaattgcgc tgggatccct tcatcgatct cgaccggcag
     2161 catcacctgg tcgaggcggc gcatgcggcg ctgacccgcg tcgggctcga ccacatcgcg
     2221 gagcggccgc tggcgcagac ctcctacggc gagcgccggc gggtggagat cgcgatggcg
     2281 ctggcgcaga atccgaaggt cctgctgctc gacgagccgt tcgcgggact gtcgatcgac
     2341 gagcggcgcg acgtgcagca attgctgctc gcgattccgc gcgacgtgac catcgtcatg
     2401 atcgagcaca acatggacgt ggcgctggaa ttcgccgagc gcatcacgct gctgcatttc
     2461 ggcgaggtga tcgtcgaggg ctcgcgcgcc gaggtggtgg ccgatccccg cacccgagag
     2521 gtctaccttg gccactagcg cgctccgtct cagcgacgtc gatgccttct acggggacag
     2581 ccacgtcctc gagaaggtgt cgttcgcggc gggcgagggc cggctgctcg gcctgctcgg
     2641 ccgcaatggc gcgggcaagt cgacctgcat gaacgtgacg gtcgggctct tgccggcgcg
     2701 tcgcggctcg gtcgaggtgt tcggcgtgcc ggtgacgcgg ctgccgcccg aacagatcgc
     2761 ggcgcagggc g
//
LOCUS       DASPXW010000197         2029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_435605, whole genome shotgun sequence.
ACCESSION   DASPXW010000197 DASPXW010000000
VERSION     DASPXW010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435605"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VEL04_04675"
     CDS             complement(<1..597)
                     /locus_tag="VEL04_04675"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYT47690.1"
                     /translation="MLRTLTFFLAALLAPALVTPAAAQDYPVREIRSICNFGAGSGAD
                     IVVRYYSERLSRIAGKPVVVENKPGANGNVGADFVAKSAPDGYTLLLTDLGGLVISAS
                     VYPQLPFNPAKDFTPVIMVSYSPHVLAVHPSVPVKDVRELIAMAKAQPGKLNFAISGI
                     GGAPHLAGIEFAQRTGVLWTYIPYKGGSDAVAAVAGGQA"
     gene            599..1885
                     /locus_tag="VEL04_04680"
     CDS             599..1885
                     /locus_tag="VEL04_04680"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HYT47691.1"
                     /translation="MANIPRRVAILGGARIPFARANSAYAEASNQDMLTAAFGFLVDK
                     YSLKNEKLGEVAAGAVIKHSRDWNLARESALGAGLHPTTPAYDLQRACGTSLSAVAQL
                     AHRIAAGEIDSGIAGGADSASDIPLNYGTKLQRAVLKLSRSRSLGERLATIGSIRPRD
                     LKPAHPGVGEPRTGMSMGEHCEVMAKEWQIGRAAQDQLALASHQKAAAAWKDGFFKDL
                     VFEFGGLKSDNNVRADTSLEKLSSLKTSFDRSAAGTLTAGNSSPLTDGASCVLLASEE
                     WAVARKIPVQAYVAFTETAAVDFVGMAGPQEGLLMAPAYAVPRMLDRAGIRLQDFDIY
                     EIHEAFAAQVLCTLKAWESEDFCQKKLKRPALGSIDRAKLNPKGSSVALGQPFAATGT
                     RIVAVLAKQLAARGSGRGLISICTAGGMGVTAILER"
BASE COUNT          364 a          671 c          690 g          304 t
ORIGIN      
        1 ggcctgcccg ccggcgacgg cggccacggc gtccgagccg cccttgtacg gaatataggt
       61 ccatagcacg ccggtgcgct gggcgaactc gatgcctgcg agatgcggcg cgccgccgat
      121 accggagatg gcgaagttca gtttcccggg ctgcgccttc gccatcgcga tgagctcgcg
      181 cacgtctttc accggcaccg atgggtgcac ggcgagcacg tgcggcgaat aagacaccat
      241 gatcaccgga gtgaaatcct tggcgggatt gaacggcagt tgcggataga cgctcgcgct
      301 gatgacgagg ccgccgagat cggtcagcag cagcgtgtag ccgtccggcg cgctcttggc
      361 cacgaaatcg gcgccgacgt tgccgttggc accgggcttg ttctcgacga ccacgggctt
      421 gcccgcgatg cgcgagagcc gctcgctgta gtagcgcacc acgatgtcgg cccccgagcc
      481 cgcgccgaag ttgcagatcg agcggatctc gcgcacgggg tagtcctgcg ccgccgccgg
      541 ggtgaccaat gcgggcgcga gcagcgcggc cagaaaaaag gttagagtcc tgagcaccat
      601 ggccaacata ccacgacgtg tcgcaatact gggcggcgcc cgcattccgt tcgcgcgcgc
      661 caacagcgcc tatgccgagg ccagcaacca ggacatgctg accgcggctt ttggatttct
      721 ggttgataaa tattctctca agaacgaaaa gctgggcgag gtcgccgccg gcgcggtgat
      781 caagcactcg cgcgactgga acctggcgcg cgagtcggcg ctcggcgccg ggctgcatcc
      841 caccacgccg gcgtacgacc tgcagcgcgc ctgcggcacc agcctctcgg cggtggcgca
      901 gctcgcgcac cgcatcgccg cgggcgagat cgactcgggc atcgccggcg gcgccgacag
      961 cgcgagcgac atcccgctca actacggcac gaagctgcag cgcgcggtgc tgaagctctc
     1021 cagaagcagg tccctgggtg aaaggctcgc caccatcggc tcgatccggc cgcgcgatct
     1081 caagccggcg catcccggcg tcggcgagcc gcgcaccggc atgtcgatgg gcgagcactg
     1141 cgaggtgatg gcgaaggaat ggcagatcgg ccgcgccgcg caggaccagc tcgcgctggc
     1201 gagccaccag aaggccgcgg cggcgtggaa ggacgggttt ttcaaggacc tggttttcga
     1261 gttcggcggc ctgaagagcg acaacaacgt tcgcgccgat accagtctgg aaaaactgtc
     1321 ttcgctgaag acttcattcg atcgcagtgc cgcggggacg ctcaccgccg gcaacagctc
     1381 gccgctcacc gatggtgcgt cctgcgtgct gctcgcgtcg gaagaatggg ctgttgcaag
     1441 aaagattccc gtccaggcgt atgtggcctt tacggaaacc gcagccgtcg acttcgtcgg
     1501 catggcaggt ccgcaggagg gtctgctgat ggcgccggcc tacgcggtgc cgcgaatgct
     1561 cgaccgcgcc ggcatcaggc tgcaggactt cgacatctac gagatccacg aggccttcgc
     1621 cgcgcaggtg ctgtgcacgc tgaaggcctg ggagtcggaa gatttctgcc aaaagaaact
     1681 gaagcgtccc gccctcggca gcatcgaccg ggcgaaactg aatccgaaag gctcgagcgt
     1741 cgcgctcggc caaccgttcg ccgccaccgg cacgcgcatc gtcgccgttc tcgccaagca
     1801 gctcgccgcg cgcggctcgg gccgcggcct gatctcgatc tgcaccgccg gcggcatggg
     1861 cgtcaccgcg atcctcgagc gctgaccccc tgaccccctg acaagggggt cgcgagcatt
     1921 gcgagcgggg ggttagaaca gggtcgcgag catcgcgagc gggggattag aacatcacct
     1981 cgagccccag cgccgggccc gagaagcgca gcgtgatcgg cgaaaggtt
//
LOCUS       DASPXW010000198         4223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_688269, whole genome shotgun sequence.
ACCESSION   DASPXW010000198 DASPXW010000000
VERSION     DASPXW010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688269"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="VEL04_04685"
     CDS             <1..1021
                     /locus_tag="VEL04_04685"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HYT47692.1"
                     /translation="GVTSLACPPDTDPPLDEPGLVDMLRRRAKALMRARVYPIGALTV
                     KLKGETLTEMAELTEAGCVAFSQANVPLADTQVLWRALQYAATFGFPVWLRVEDAWLA
                     KGGVSHDGEVATRLGLPGIPAFAETIALGALLELVRATGARVHVCRLSTAGAIELMRR
                     AKQERLPISCDVAIHHVHLCDMDLGYFDSQCRLVPPLRSQRDRDALASALADGVIDCL
                     CSDHTPVDEDGKHLPFDEAEPGATGLELLLPLTLKWGEARKLPLAATLARITSEPARI
                     LGVASGRLAADAPGDLAIFDSRASLRIAPESLKSQGKNTPFLGYELAGKVRYTIVAGN
                     VVYEA"
     gene            1033..1530
                     /locus_tag="VEL04_04690"
                     /pseudo
     CDS             1033..1530
                     /locus_tag="VEL04_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418797.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
     gene            1607..2728
                     /locus_tag="VEL04_04695"
     CDS             1607..2728
                     /locus_tag="VEL04_04695"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008453065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HYT47693.1"
                     /translation="MRFSVLALCLLCAAARAAAPLPPSVIGRAWLVGDLSSGQILTSQ
                     KPDERIEPASLTKLMTAYLVFAALRDKKLTLGQQAPVSERAWRAPGSRMFIEPRRPVS
                     VDELIRGMEVQSGNDACIALAEAVAGSEEVFVQLMNREAARLGLKDSHFMNATGLPDA
                     QHYSTAADLYRLAAALIRDFPVEYAQYYAQKEFRYNNITQPNRNRLLWLDPTVDGVKT
                     GHTEAAGFCLIASSKRGARRLLSVLLGSTSEATRAQDSLKLLNWGFQFFDSVRLYGAG
                     DAVKSIDVWKGAASAVKVGPQGDLFVTVPKGEAGKLKAELVSQQPLIAPLARGQRVGS
                     LRVSFEGKPYAEHPLVALESVGPAGFFGRAWDTLRLWVK"
     gene            2740..3609
                     /locus_tag="VEL04_04700"
     CDS             2740..3609
                     /locus_tag="VEL04_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HYT47694.1"
                     /translation="MVFLNGKFVPIDEARVSVLDRGFIFGDGVYELVPVYSRVPFRLD
                     EHLARLERSLAEARIRNPHSRAQWRKIIYQLIDAQPFDDQGVYFQVTRGVAKRDHAFP
                     KNAEPTVFMMSNPLVNPPQPQVEGGAAAVSAQDNRWLRCDIKSISLIGNVLLRQLSAE
                     ADAAETILFRDGRLTEASASNVFIVARGLILSPPKSSLILPGITYDVVAELARASAMP
                     LEFREVTDAEVRAADEIWVTSSSKEVLAIVSLDGRKVGEGRPGPVFRRMYALYQQFKQ
                     KVMRAGKREALSA"
     gene            3610..3873
                     /locus_tag="VEL04_04705"
     CDS             3610..3873
                     /locus_tag="VEL04_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HYT47695.1"
                     /translation="MPEGLPLAYPCDFPIKVVGRKHPGFAQTVMEIVMKHAPDFDPAS
                     IEMRPSRQGRYLSVTCVLRATSREQLDTLYQELCDQPSVVMVL"
     gene            3870..>4223
                     /gene="lipB"
                     /locus_tag="VEL04_04710"
     CDS             3870..>4223
                     /gene="lipB"
                     /locus_tag="VEL04_04710"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:TIGR00214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016979 - lipoate-protein ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HYT47696.1"
                     /translation="MTLVVDAVLKRLGQVPYTPTVEAMRAFTARRDDTTPDELWILEH
                     PPIYTLGQGAAEAKISNEIAVVRSDRGGEITYHGPGQVVLYTLVDLARRSIKVKRFVW
                     LLEQAVIDLIGNRAER"
BASE COUNT          703 a         1427 c         1399 g          694 t
ORIGIN      
        1 cggggtgacg agccttgcct gcccgccgga taccgatccg ccgctcgacg agccggggct
       61 cgtcgacatg ctgcgccggc gtgccaaggc gctgatgcgc gcgcgcgtct atccgatcgg
      121 cgcgctcacc gtgaagctca agggcgagac cctcaccgag atggccgagc tcaccgaggc
      181 aggctgcgtc gctttctcgc aggcgaacgt gccgctcgcc gacacgcagg tgctgtggcg
      241 cgcgctgcaa tacgcggcga ccttcggctt tccggtatgg ctgcgggtcg aggacgcctg
      301 gctcgccaaa ggcggtgtgt cgcacgacgg cgaggtggca actcgcctcg gcctgcccgg
      361 catcccggcc ttcgccgaga ccatcgcgct tggcgcgttg ctcgagctgg ttcgcgccac
      421 cggcgcgcgc gtgcacgtat gccggctttc gactgccggc gcgatcgagc tgatgcgccg
      481 cgcgaagcaa gagagattgc caatcagctg cgatgtcgcc atccaccatg tgcacctctg
      541 cgacatggac ctgggctatt tcgattcgca gtgccggctg gtgccgccgc tcagaagcca
      601 gcgcgaccgc gatgcgctcg ctagcgcgct cgccgacggg gtaatcgact gcctgtgctc
      661 cgatcacacg ccggtcgacg aggacggcaa gcacctgccg ttcgacgaag ccgaacccgg
      721 ggcgacgggc ctcgagttgc tgctgccgct gacactgaaa tggggcgagg cgagaaagct
      781 gccgctcgcg gcgacgctcg cccgcatcac ctccgagccg gcgcgcatcc tgggcgtggc
      841 ttccggccgg ctcgctgccg atgcgcccgg cgacctcgcg atcttcgact ccagggcgtc
      901 gctgcgcatc gcgcccgagt ccctcaagag ccagggcaag aacacgccct tcctcggcta
      961 cgagctcgct ggcaaggtgc gttacaccat cgtcgccggc aacgtggtct acgaagcgtg
     1021 aggacggttt tcatcgtctg gctgctttcc ctgctcggcg cttgcgcctc ggctccggag
     1081 agaagcggcg ggtattacaa ggacgacggt ccgggtgacg cagcgacgct gaacctggcc
     1141 gcgattcccg acgcgcagcc gaaggtggag ccgctgcacc gcttcgccaa ccgtccgtac
     1201 aaggtcctcg gcaaggaata cgtaccgctc gccgcgtctc agccctatca gcagcgcggc
     1261 gttgccagct ggtacggcaa gaaattccac ggccaggcca cttccagcgg cgagcgctac
     1321 gacatgtacg cgatgaccgc ggcgcacacg acgctgccga ttccgagcta tgcccgtgtc
     1381 acgcacctgc gcagcgggcg cagcgtcgtg gtgcgcatca acgatcgcgg cccgttccgt
     1441 ggcaaccgca tcatcgatct ctcctatgcg gcggcataca agctcggcat caccaaggcg
     1501 gggagcgccg aggtcgaagt cgagagcatc aatcaaagat ccggacctcg ggcctcgagc
     1561 tccgctagcc tggccttata atccgctcct tttttcggtc cgattcatgc gattcagcgt
     1621 tctcgcccta tgtctactct gcgccgcggc gcgcgccgcg gcgccgctgc cgccgtcggt
     1681 catcggcagg gcctggctgg tgggcgatct gtcgagcggc cagatcctca cctcgcagaa
     1741 gcccgatgag cgcatcgaac ctgcttcgct caccaagctg atgacggcct acctggtatt
     1801 cgccgcgctg cgcgacaaga agctgacgct tggccagcag gcgccggtgt cggagcgcgc
     1861 ctggcgcgcg cccggctcgc gcatgttcat cgagccacgc cggccggtca gcgtcgacga
     1921 gctgatccgc ggcatggagg tgcagtcggg caacgatgcc tgcatcgcgc tcgccgaggc
     1981 ggtggcgggc tcggaagagg tgttcgtcca gctgatgaac cgggaggcgg cgcgcctggg
     2041 cctgaaggac agccatttca tgaacgccac tggcctgccc gatgcgcagc actacagcac
     2101 cgccgccgac ctgtaccggc tcgccgcagc gctgatcagg gactttcccg tcgaatacgc
     2161 gcagtactac gcgcagaagg agtttcgcta caacaacatc acgcagccga accgcaaccg
     2221 cctgctgtgg ctcgacccga cggtggacgg cgtcaagacc gggcacaccg aggccgcagg
     2281 cttttgcctc atcgcgtcct cgaagcgcgg cgcgcgacgc ctgctttccg tgctgctcgg
     2341 ctcgacctcg gaggcgacgc gcgcgcagga ctcgctcaag ctgctcaact ggggcttcca
     2401 gttcttcgac agcgtcaggc tctacggcgc cggcgacgcg gtgaaatcca tcgacgtctg
     2461 gaagggcgcc gcgagcgcgg tgaaagtcgg cccacagggc gatctgttcg tcaccgtgcc
     2521 gaaaggcgag gccgggaagc tgaaggccga gctggtgtcg cagcagccgc tgatcgcgcc
     2581 gctcgcccgc ggccagcgcg tcggcagcct gcgcgtcagc ttcgagggca agccgtacgc
     2641 tgaacacccg ctggtcgcgc tggagagcgt cgggccggca ggattcttcg gccgcgcctg
     2701 ggatacactg cggctgtggg taaagtagcg aggcgcgtga tggttttcct gaacggcaaa
     2761 ttcgtcccga tcgatgaggc cagggtctcg gtgctggatc gcggcttcat cttcggcgat
     2821 ggcgtctacg agctggtgcc ggtgtactca cgcgtgccgt tccggctcga cgagcacctg
     2881 gcgcggctcg agcgcagcct tgccgaagcg agaatccgca acccgcactc gcgcgcgcag
     2941 tggcgcaaga tcatctacca gttgatcgac gcgcagccct tcgatgacca gggcgtgtac
     3001 ttccaggtca cgcgcggcgt ggcgaagcgc gaccatgcgt ttccgaaaaa tgccgagccg
     3061 accgtattca tgatgtcgaa cccgctggtg aacccgccgc agccgcaggt ggaggggggc
     3121 gccgccgccg tcagcgcgca ggacaaccgc tggctgcgct gcgacatcaa gtcgatctcg
     3181 ctcatcggca atgtactgct gcgccagctc tccgccgagg cggacgccgc cgagacgatc
     3241 ctgtttcgcg acggcaggct gaccgaggcc tcggcgtcga acgttttcat cgtcgcgcgc
     3301 ggcctgatcc tgtcgccgcc gaagtcgagc ctgatcctgc cagggatcac ctatgacgtg
     3361 gtggccgagc tcgcgcgcgc aagcgccatg ccgctcgagt tccgcgaggt aaccgacgcc
     3421 gaggtgcgcg ccgccgacga gatctgggtc acctcttcct cgaaggaggt gctcgcgatc
     3481 gtctcgctcg acggccgcaa ggtcggcgaa ggccggccgg gaccggtgtt ccggcgcatg
     3541 tacgcgctgt accagcaatt caagcaaaag gtgatgcgtg ccggcaagcg ggaagcatta
     3601 tcggcataga tgccggaggg cttgccgcta gcctacccct gcgatttccc catcaaggtg
     3661 gtggggcgca agcacccggg cttcgcgcag accgtgatgg aaatcgtgat gaagcacgca
     3721 ccggatttcg acccggcgag catcgagatg cgacccagcc ggcagggacg ctatctgagc
     3781 gtgacctgcg tattgcgcgc cacctcgcgc gagcagctcg acacgctcta ccaggagctg
     3841 tgcgaccagc cttcggtggt gatggtgcta tgacgctcgt cgtagacgca gtcctgaaaa
     3901 ggctcggcca ggtgccgtac accccgaccg tcgaggcaat gcgcgctttc actgcacgac
     3961 gcgacgacac gacgccagac gagctctgga ttctcgagca ccccccgatt tacacgttgg
     4021 ggcaaggtgc ggcggaagcc aaaatctcca atgagattgc tgtcgtacgt tctgaccgcg
     4081 gcggcgagat cacctaccac ggcccggggc aggtggtgct ctacacgctg gtcgatctcg
     4141 cgcggcgctc gatcaaggtc aagcggttcg tgtggctgct cgagcaggcg gtgatcgacc
     4201 tcatcggcaa ccgcgccgag cgc
//
LOCUS       DASPXW010000199         2220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_59865, whole genome shotgun sequence.
ACCESSION   DASPXW010000199 DASPXW010000000
VERSION     DASPXW010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59865"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1185)
                     /locus_tag="VEL04_04715"
     CDS             complement(<1..1185)
                     /locus_tag="VEL04_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47697.1"
                     /translation="MKAKLVAALVASALLLPVSGVYGHDDDAKGEKFGKVLFKTSCSP
                     AAQKEFERALALLHSFDFPETIKAFSAIPQTDPGCAIAYWGLAVSIRPNPLVGPWDAA
                     TLKRGLDAVEKGEAIGAKTQRERDWLAAIKEFYKGFDTVDQDTRTRNYEKAMEALTRK
                     YPNDVEAKVFHALALNEIFDHKDMKPLLQAIKILQPLERQYPNHPGIAHYLIHSYDFA
                     PLAKKGVPVANKYAKIAPSAAHAQHMPSHIYSMVGMWKESIASNISSIKVANEYAAKA
                     KLDGVTVGVPHSYDFMQYAYLQLGQDAKAKELIAGTAKVTKVLGPVSAGQMARAAVPA
                     RYTLERQDWKGAAQLQPLGSPFPAAEAVTHFARALGAARSGELAAAQADIEKLKELRA
                     GLE"
     gene            1258..>2220
                     /gene="glnS"
                     /locus_tag="VEL04_04720"
     CDS             1258..>2220
                     /gene="glnS"
                     /locus_tag="VEL04_04720"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HYT47698.1"
                     /translation="MSTSNFIRQIVEADLAASAYPGGIRTRFPPEPNGYLHFGHAKAI
                     CLNFGLAEQYGGRCNLRFDDTNPTKEEQEYVDAIKDAVRWLGFDWGSHEYYASDYFEK
                     LFQFAEALIERGHAYVDSQSVEETRAARGSFTEHGVDSPFRTRSPQESLRLLREMKDG
                     KHLDGSHVLRAKIDMRSPNINLRDPVMYRIRHATHHRTGDKWCIYPTYDWAHGLSDCI
                     EGVTHSICTLEFEDHRPLYDWFNERVVEAGLLKRPLPKQIEFARLNLTYVVLSKRMLI
                     ELVDEKHVDGWDDPRMPTLAGARRRGYTPEGLRRFADKIGVSKAH"
BASE COUNT          386 a          762 c          670 g          402 t
ORIGIN      
        1 ctccaggccg gcgcgcagct ccttcagctt ctcgatgtcc gcctgcgcag cggcgagctc
       61 gcccgagcgc gccgcgccca gggcgcgcgc gaaatgcgtc accgcctcgg ccgccgggaa
      121 cggcgagccg agcggctgca gctgcgcggc gcccttccag tcctggcgct cgagcgtgta
      181 gcgcgccggg acggcggcgc gcgccatctg cccggccgac accggcccca gcactttggt
      241 gactttcgcg gtcccggcaa tcagctcttt cgccttcgcg tcctgaccga gctgcaggta
      301 ggcgtactgc atgaagtcgt acgagtgcgg cacgcccaca gtgacgccgt cgagcttggc
      361 cttcgcggcg tattcgttcg ccacttttat cgagctgatg ttcgaggcga tcgattcttt
      421 ccacatgccg accatcgagt agatgtgcga cggcatgtgc tgcgcatgcg cggccgacgg
      481 cgcgatcttc gcgtacttgt tggctaccgg cacgcccttt ttcgcgagcg gcgcgaaatc
      541 gtagctgtgg atcagataat gcgcgatgcc tgggtggttc gggtattgcc tctcgagcgg
      601 ctggaggatc ttgatcgcct gcagcaacgg cttcatgtcc ttgtggtcga agatctcgtt
      661 cagcgcgagc gcgtggaata ccttggcctc gacgtcgttc ggatacttcc tggtcagagc
      721 ctccatcgcc ttctcgtagt tcctggtgcg cgtgtcctga tcgacggtgt cgaagccctt
      781 gtagaactcc ttgatcgccg cgagccagtc gcgctcgcgc tgcgtcttgg cgccgatcgc
      841 ctcgcccttc tccaccgcgt cgaggccgcg cttcagcgtc gccgcgtccc agggcccgac
      901 cagcggattg ggccggatgc tgaccgcgag cccccagtag gcgatggcgc agcccggatc
      961 ggtctgcggg attgccgaga acgccttgat ggtctccggg aaatcgaacg aatgcagcag
     1021 cgcgagcgcg cgctcgaact ccttctgcgc cgccgggctg cacgaagtct tgaacagcac
     1081 cttgccaaac ttctcgcctt ttgcatcgtc gtcatgccca tatacgccgg aaacgggcaa
     1141 caacaatgcg gatgcaacca gcgccgcaac gagtttcgct ttcatggttc ccctcctgtc
     1201 cgggcgcaaa gtttacgccg taagctagaa tctggaaacc ccagtcgatt cagcaggatg
     1261 tccacgtcca atttcattcg ccagatcgtc gaggcagacc tcgccgcgag cgcctacccc
     1321 ggtggcatcc gcacccgctt tccgcccgag ccgaacggct acctgcactt cggccacgcc
     1381 aaggcgatct gcctcaactt cggcctcgcc gagcagtacg gcgggcgctg caacctgcgc
     1441 tttgacgaca ccaacccgac gaaagaagag caggagtacg tcgacgccat caaggacgcg
     1501 gtgaggtggc tcggtttcga ctggggcagc cacgagtact acgcctcgga ctatttcgaa
     1561 aagctcttcc agttcgccga agcgctgatc gagcgcggcc atgcctacgt cgacagccag
     1621 agcgtcgagg agaccagggc tgcgcgcggc agctttacag agcacggcgt cgacagtcct
     1681 ttccgcaccc gatctcccca ggaatcgctt cgcctgctgc gtgaaatgaa agacggtaaa
     1741 cacctggacg gctcgcatgt gctgcgcgcg aagatcgaca tgcgctcgcc caacatcaac
     1801 ctgcgcgacc cggtgatgta ccgcatccgc cacgccacac atcaccgcac cggcgacaag
     1861 tggtgcatct acccgaccta cgactgggcg cacggcctgt ccgactgcat cgagggcgtc
     1921 acgcactcga tctgcacgct ggaattcgaa gaccaccggc cgctctacga ctggttcaac
     1981 gagcgcgtcg tcgaggccgg cctgctcaag cgcccgcttc ccaagcagat cgagttcgcc
     2041 cggctgaatc tcacctacgt cgtcctgagc aaaagaatgc tgatcgagct ggtcgacgaa
     2101 aagcacgtcg acggctggga cgacccgcgc atgccgacgc tcgccggcgc gcgccgccgc
     2161 ggctacacgc ccgagggctt gcggcgcttc gccgacaaga tcggcgtctc caaggcgcac
//
LOCUS       DASPXW010000200         5911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_939312, whole genome shotgun sequence.
ACCESSION   DASPXW010000200 DASPXW010000000
VERSION     DASPXW010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5911
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939312"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="VEL04_04725"
     CDS             <1..662
                     /locus_tag="VEL04_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018574012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HYT47699.1"
                     /translation="QPLGCFKKPMTKPDDFKGLKYRTVGISIDLFQGLGAAVNALPGG
                     EIVPAMDRGLLDAAEFNNASSDRLLGFPDVSKVYMLQSYHQSGEQFEITFNKTKFDAL
                     PARMKTIIENAVEAASQDLQWKSIDRYSKDYIEMQTKQNVKVYITPPSILQRQLEIFD
                     AVMAKYSATNPLFKEVIESQRAFAQRAVKWGLDTIVSPRMAYNYYFGKKPAAGASKKK
                     A"
     gene            767..1390
                     /locus_tag="VEL04_04730"
     CDS             767..1390
                     /locus_tag="VEL04_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HYT47700.1"
                     /translation="MQKLLLFVDKVSTWVGHAFSWFIVGLTLLISWEVFSRYALDTPH
                     AWAFDAMIQMYGTLFMMSGAYTLAKNGHVRGDVVYGFFTPRAQASLDLTLYIVFFIPG
                     VVALVWAGYTYAAESWAIRESSNITSEGPPYYPFKTIIPVAGAFVLVQGIVEIIRCAI
                     CLKQGDWPSREEDVQEVDVDKLKEMVHVKDEDIQQLDAYVVHQEPRK"
     gene            1393..3372
                     /locus_tag="VEL04_04735"
     CDS             1393..3372
                     /locus_tag="VEL04_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HYT47701.1"
                     /translation="MRKELWFGFSLMALIILPVIVLMPWGHLTNGHLGLLMLALICVG
                     IMLGFPTAFTLMGMGVFFAWLAYRSVNPALANRQVLDLFVQRTYGVMSNDVLIAIPLF
                     LFMGYLVERAQLIDRLFRSLHLATSRIPGSLAVATIVTCAIFATATGIVGAVVTLMGL
                     LAFPAMLKAGYNVKVAAGAVTAGGCLGILIPPSVLLIVYGAVAGVSVVQLYAGAFLPG
                     FMLAGLYIGYVIVLAKWKPNLMPPLAESERRVQLPAFAQALAPHGGNALSGLWRAVTG
                     GNAGVAKHTALAQLLVTLVPALFIAALLGITYRVTTAPEVEASTAGLIEVGGLVAAPE
                     LEREASTGLIGAPEAEEKDPEIKQPPAEEAKQPVPEKPAATAAAAAEHERLPVPPWLW
                     IAFGVGIGAVAVIYRLWNWERLEVFKMLLSSFFPLALLILAVLGSIVFGLATPSEAAA
                     VGGFGGFVLAGVYRFIAHMREGESGGKDAGKAIWTTVKELGSIVKESSFLTAKTSAMV
                     CWLFVGSGIFSAAFALLGGQEVIEKWVLSLDMTPVQFMILAQFIIFILGWPLEWTEII
                     IIFMPIFLPLLPHFHIDPLFFGLLVALNLQTAFLSPPVAMAAFYLKGVSPPHVTLNAI
                     FAGMMPFMGIQVIALVILYVFPQIGLWLPSVVYGR"
     gene            3442..4713
                     /locus_tag="VEL04_04740"
     CDS             3442..4713
                     /locus_tag="VEL04_04740"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HYT47702.1"
                     /translation="MLTSEELVAACLERIRAAEPSVQAWAFLDEEHALEQARAADERK
                     RSGEPIGALHGVPVGLKDIIDTADMPTENGTVLHKGRTPRSDAAVVASLRAAGAVILG
                     KTVTTECAYFSPGKTRNPHNPEHTPGGSSSGSAAAVAAAMVPLALGSQTAGSTIRPGS
                     FCGVYAFKPTHGLIPRTGILQLSRTLDHVGLFARSIEDVALLMEQLIGYDEGDPDTRP
                     RARLPFQAICSEAPPLEPMLAFVKTPHWERADVDTKEAFSELLETLGDRAEELELFPS
                     AAEAWDWHKTIMQAEMAANFEREWQTGRDKLSPQLRALMEKGREVRAVDYQRALRRIP
                     RLVESFDELFMERYDAILTPAALGTAPKGLGATGDPVFCSLWTLLGMPAVSLPLMQGA
                     NGLPLGVQLVGRRNFDARLLRTARWLAARAA"
     gene            complement(4741..5688)
                     /locus_tag="VEL04_04745"
     CDS             complement(4741..5688)
                     /locus_tag="VEL04_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47703.1"
                     /translation="MQDRLNQNDVSNRINVEDSNEVRDAVLQPFAARYPGTDCSVVAR
                     AFDDFRALFEGSFPGYLACDTLYHDRRHTLDMTLAVARLIDGHDRACAESDRLGARRA
                     MLGVLVALLHDSGYLKRSSEAEVENGAIFTKVHVSRSADFISAYLPKLGFAAETPLAA
                     RLVHFTGYEMDLDDIRVEDARDRMIGCMVGTADLVGQMSDRMYLEKVRQFLYEEFVWG
                     KIAREQLPDGREVVRYASADDLIIKTPGFYEYVARARMEKKLGAVDRYAAAHFDGPDL
                     YQTEIDHNMDFLRHAIEKAELHRLRRYCYSLSSSARRAA"
BASE COUNT          987 a         1892 c         1960 g         1072 t
ORIGIN      
        1 cgcagccgct cggctgtttc aagaagccga tgaccaagcc cgacgacttc aagggcctca
       61 agtaccgaac cgtgggcatt tcaattgacc tgttccaggg cctgggcgca gcggtgaacg
      121 cgctgcccgg cggcgagatc gtgccggcga tggatcgggg cctgctcgat gcagcggagt
      181 tcaacaacgc gagttccgac cgcctcctcg gcttccccga cgtgtccaag gtgtacatgc
      241 tgcaaagcta ccaccagagc ggcgagcagt tcgagatcac gttcaacaag accaagttcg
      301 atgcgctgcc ggccaggatg aagacgatca tcgagaatgc cgtggaggct gcgtcgcagg
      361 acttgcagtg gaaatcgatc gaccgctact cgaaggacta catcgagatg cagaccaagc
      421 agaatgtgaa ggtctacatt actccgccgt ccatactgca aaggcagctc gagattttcg
      481 acgcggtcat ggcgaagtat tcggcgacga acccgctgtt caaggaggtc atcgaatcgc
      541 agagggcatt cgcccagcgc gcggtgaagt ggggtctcga caccatagtc agtccacgca
      601 tggcctataa ctattatttt ggcaagaagc cggccgccgg agcgagcaag aagaaggcct
      661 gaacgcaagc gaaaagtcag gaatcacaga acaccatccg gtaaaatcac cgggtggtgt
      721 tctttttttg tgggggcggt gatttgccgc ccgagggaga gacagcatgc agaaactgct
      781 tcttttcgtt gacaaggtga gcacctgggt cgggcacgcg ttttcctggt tcatcgtggg
      841 gcttaccttg ctcatttcct gggaagtatt ctcgcgttac gcgctcgaca caccgcatgc
      901 gtgggctttc gacgcgatga tccagatgta cggaaccctc ttcatgatgt ccggcgccta
      961 cacgctggcc aagaacggcc atgtgcgggg ggacgtcgtc tacggcttct tcacgccgcg
     1021 ggcgcaggcc agtctggatc ttaccctcta cattgtgttc ttcattcccg gtgtcgtcgc
     1081 gctcgtctgg gcgggctaca cctacgccgc cgagtcgtgg gcgatcaggg agagctcgaa
     1141 catcacatcc gagggtccgc cgtactaccc gttcaagacc atcatcccgg tcgccggcgc
     1201 gttcgtgctg gtgcagggca tcgtcgagat catccgctgc gccatctgcc tgaagcaggg
     1261 tgactggccc tcgcgcgaag aggacgtcca ggaagtcgat gtcgacaagc tcaaggagat
     1321 ggtccacgtc aaggacgagg acatccagca gcttgacgcg tatgtcgtgc accaggagcc
     1381 gcgaaagtga agctgcgcaa ggagctgtgg ttcggcttct cgctgatggc gctcatcatc
     1441 ctcccggtga tcgtgctcat gccctggggc cacctcacga acgggcacct ggggctcctc
     1501 atgctcgcgc tgatctgcgt cggcatcatg ctcggctttc ccacggcctt tacgctgatg
     1561 gggatgggcg tgttcttcgc ctggctcgcg taccgcagcg tcaaccccgc gctcgcgaac
     1621 cggcaggtgc tcgacctttt cgtgcagcgc acctatggcg tgatgtcgaa cgacgtgctg
     1681 atcgcgattc cgctgttcct attcatgggc tatctggtcg agcgggcgca gctgatcgac
     1741 cggctgttca ggagcctgca tctcgctacc tcgcgcatac ccgggtcgct cgcggtggcg
     1801 accatcgtca cctgcgcgat cttcgccacc gccaccggca ttgtcggcgc ggtggtgacg
     1861 ctgatgggcc tgctcgcgtt tccggcgatg ctcaaggccg gatacaacgt caaggtggcg
     1921 gcgggcgcgg tcacggcagg cggctgcctc ggcatcctga tcccgccgtc ggtgctgctg
     1981 atcgtctacg gcgccgttgc aggcgtgtcg gtggttcagc tctacgccgg cgctttcctg
     2041 cccggcttca tgctcgccgg gctttacatc ggttacgtca tcgtgctcgc gaagtggaag
     2101 cctaacctca tgcctccgct tgccgagagc gagcggcgcg tgcagcttcc ggccttcgcg
     2161 caggctctgg cgccgcatgg cggcaacgca ttgagcgggc tgtggcgcgc agtgactggc
     2221 ggaaacgccg gtgtcgcaaa acacaccgcg cttgcgcagc tgcttgtcac gctggtgccg
     2281 gcgctgttca ttgccgcttt actgggcatc acttatcggg tgacgacggc gccggaagtc
     2341 gaagccagca cggccgggct gatcgaagtc gggggtctcg tcgcggcgcc cgagctcgaa
     2401 agggaggcgt cgacaggcct catcggcgcg cccgaagcgg aggaaaagga tccggaaatc
     2461 aagcaaccgc cggccgaaga ggcgaaacag cctgtacccg agaaacccgc cgcaacagcc
     2521 gccgcggccg cggagcacga gaggctgcca gtgccgccat ggttgtggat cgcgttcggc
     2581 gtcggcatcg gcgcggtcgc ggtcatttac cggctctgga actgggagcg gctcgaggta
     2641 ttcaagatgc tgctctcctc cttctttccg ctggcgctgc tcatccttgc ggtgctcggc
     2701 tcgatcgtgt tcgggctggc gacgccctcc gaggctgcgg cggtcggcgg cttcggcggc
     2761 ttcgtcctgg cgggggttta ccgcttcatc gcgcatatgc gcgaggggga gtccggcggc
     2821 aaggatgccg gcaaggcgat ctggaccacg gtgaaggagc tcggcagcat cgtcaaggaa
     2881 tcgtccttcc tcaccgccaa gaccagcgcg atggtgtgct ggctgttcgt cggctccggc
     2941 attttctccg ccgcgttcgc gctgctcggc ggtcaggaag tcattgagaa gtgggtgctg
     3001 tcgctcgaca tgaccccggt gcagttcatg atcctcgcgc agttcatcat cttcatcctc
     3061 ggctggccgc tcgagtggac cgagatcatc atcatcttca tgccgatttt cctgccgctg
     3121 ctgccgcact tccatatcga cccgctgttc ttcggcctgc tggtggcgtt gaacctgcag
     3181 accgcgttcc tgtcgccgcc ggtggcgatg gcggccttct acctgaaagg cgtatcgccg
     3241 ccgcatgtga ccctcaacgc gattttcgcc ggcatgatgc cgttcatggg gattcaggtc
     3301 atcgccctgg tgatcctgta cgtgttcccg cagatcggcc tctggctgcc aagcgtcgtg
     3361 tacgggcgct agcgcttcgt gcaagcagtt tcgcccgcct cgctagcggc caccgaggcc
     3421 gcgagaaaaa tccgcgaagg cttgctgacg tccgaagagc tggttgcggc gtgcctggag
     3481 cgcatccgcg cagcagaacc ctcggtgcag gcctgggctt tcctcgacga ggagcacgcg
     3541 cttgagcagg cgcgcgcggc ggacgagcgc aagcgctccg gcgagccgat cggcgcgctg
     3601 cacggcgtgc cggtggggct gaaggacatc atcgataccg ccgacatgcc gaccgagaac
     3661 ggcaccgtgc tgcacaaggg gcgcacgccg cgctcggacg cggcagtggt cgcgagcctg
     3721 cgcgccgcgg gcgcggtgat cctcggcaag acagtgacca ccgagtgcgc ctatttctcg
     3781 ccgggtaaga cccgcaaccc gcacaatccg gagcacacac cgggcggctc ctccagcggc
     3841 tccgccgccg ccgtcgcggc ggcgatggtc ccgcttgccc tgggcagcca gaccgccggc
     3901 tcgaccatcc ggcccggatc gttctgcggc gtgtacgcct tcaagccgac gcacggcctg
     3961 atcccgcgca ccgggatcct gcagctgtcg cgcaccctcg atcatgtagg cctgtttgca
     4021 cgcagcatcg aggatgttgc cttgttgatg gaacagctga tcggctacga cgagggcgat
     4081 cccgacacac gaccgcgcgc gcgcctgccg tttcaggcga tttgcagcga agcgccgccg
     4141 ctcgagccga tgctcgcctt cgtcaagacg ccgcactggg agcgcgccga cgtcgacacg
     4201 aaggaggctt tttcagaatt gctggaaacc ctgggagatc gcgccgagga gctcgagctg
     4261 tttccatcgg cagccgaggc ctgggactgg cacaagacca tcatgcaagc cgagatggcg
     4321 gcgaatttcg agcgcgaatg gcagaccggg cgcgacaagc tctcgccgca gctgcgtgcg
     4381 ctgatggaaa aggggcggga agtgcgcgca gtcgactacc agcgcgcact gcgccggatc
     4441 ccgcggctcg tcgagagctt cgacgagctg ttcatggagc gctacgacgc catcctgacg
     4501 cctgcggcgc tcggcaccgc gcccaagggc ctgggtgcga ccggagaccc ggtgttctgc
     4561 tcgctgtgga cgctgctcgg gatgccggcc gtgtcgctgc cgctgatgca gggagccaac
     4621 ggcctgccgc tcggcgtgca gctcgtcggc cgcaggaatt tcgacgcgcg gctgctgcgc
     4681 accgcgcgct ggctcgccgc ccgggccgcc tagacgcagg cgggcgcgaa aaacgcgggg
     4741 tcaggcggcg cggcgggcgc tgcttgaaag cgagtagcaa tagcgccgca ggcggtgcag
     4801 ctcggctttc tcgatcgcgt ggcgcaggaa atccatgttg tggtcgattt ccgtctggta
     4861 gaggtcgggg ccgtcgaaat gcgcggcggc gtagcggtcg accgcaccga gcttcttctc
     4921 catgcgggcg cgcgccacgt actcgtagaa ccccggggtc ttgatgatca ggtcgtcggc
     4981 ggaggcgtaa cgcaccacct ccctgccgtc gggcagctgc tcgcgcgcga tcttgcccca
     5041 gacgaactcc tcgtacagga actggcgcac cttttcgagg tacatgcggt ccgacatctg
     5101 gccgaccaag tcggcggtgc cgaccatgca gccgatcatc cggtcgcgcg cgtcctcgac
     5161 ccggatgtcg tcgagatcca tctcgtagcc cgtgaagtgc accagccgcg ccgcgagcgg
     5221 cgtttcggcg gcgaagccga gcttgggcag gtaggcggaa atgaagtcgg cgctgcggct
     5281 gacgtggacc ttggtgaaga tcgcgccgtt ctccacctcg gcttcggagc tgcgcttcag
     5341 gtagcccgag tcgtgaagca gcgcgaccag cacgccgagc atcgcgcgcc gcgcccccag
     5401 gcggtcgctc tcggcgcagg cgcgatcgtg gccatcgatc aggcgcgcca cggcgagggt
     5461 catgtcgagc gtatgccgcc ggtcgtgata cagggtgtcg caggcgagat agccgggaaa
     5521 gctgccctcg aacagcgcgc ggaagtcgtc gaaggcgcgc gcgacgacgc tgcaatccgt
     5581 gcccggatag cgcgcggcaa agggctgcag caccgcgtca cggacttcgt tcgaatcctc
     5641 gacgttgatc cggttgctga cgtcgttctg attgagacgg tcttgcatgc gcatggccgg
     5701 cgaacccccc acggtccgcc aacctcgacc tcctacccaa acgggcgatt attccgctcg
     5761 gcaggtccca aggcaaggga atcgacctac gcggtgcgtg ctgcgtggcg gcgcgaaatc
     5821 gaggggagac ggccggtaag ccgggttctg tggcccggcc gggcgaaccc gggcgggccg
     5881 gcagccattc ctctgggccg cgggttaccc c
//
LOCUS       DASPXW010000201         3208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1446303, whole genome shotgun sequence.
ACCESSION   DASPXW010000201 DASPXW010000000
VERSION     DASPXW010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446303"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="VEL04_04750"
     CDS             <1..698
                     /locus_tag="VEL04_04750"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HYT47704.1"
                     /translation="LGSILGGLATPTEAAGVGAIGALGLAAAHGRLDAARLKHALLRT
                     LATSSMVLFLAVASNVFGAVFARLGTAGWISEWLLGLSLPPMLMLLLIVALIFLLGWP
                     FEWPAIIFVFVPLFYPVVAKLGIDLVWFGALVALTLQTAFLSPPVAMSGYFLKQVAPA
                     WPLSTIFKGMMEFMGIQVIAVALLLAFPAIATWLPERLNRAAAPGIEIFDVEPRPDDA
                     GRYQAMMVRREPS"
     gene            complement(691..1311)
                     /locus_tag="VEL04_04755"
     CDS             complement(691..1311)
                     /locus_tag="VEL04_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018313230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HYT47705.1"
                     /translation="MIDLYTWSTPNGRKVSIMLEECGLPYRVHKIDISKGEQFAPDYL
                     KINPNGKIPSIVDPQGPDGKPIAMMESGAILIYLADKTGQFFSQKNKYQILQWLMFQM
                     GGVGPMFGQAHHFMRAKKDEVPYGSERYGNEAKRLYGVMDRRLEKNAFLSGSDYSIAD
                     IATYPWVARHEWHRVDLGAFAHVKRWFDAIGARPAVAKGMAVPAVS"
     gene            1321..1515
                     /locus_tag="VEL04_04760"
     CDS             1321..1515
                     /locus_tag="VEL04_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 family protein"
                     /protein_id="HYT47706.1"
                     /translation="MLKWLAIVCVVLAVAAIIAPRPGRLGLGRLPGDFQIPARGRIYY
                     IPLASTLLLSLLVWAIGKLL"
     gene            complement(1483..2832)
                     /locus_tag="VEL04_04765"
     CDS             complement(1483..2832)
                     /locus_tag="VEL04_04765"
                     /inference="COORDINATES: protein motif:HMM:NF013788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C13 family peptidase"
                     /protein_id="HYT47707.1"
                     /translation="MTERGPVVFYFHSLLRNLAAGARLALFLPVRPFDYRVSPVHFAV
                     LMGFDFAFWIAAAAVRAGFAGEFDPMALPIFFAEVSLTLAAALLAALAYREPGRLLLI
                     ATALSASDAVYELAGLALPAVAAASGYPEAAFYLLLGWTWLVAVRAVAVCGGTQRPQF
                     LLGAAAVSAMIAIGFFAFPRTDVWRAPQEEAGPDPLAGERLFHLQGRLIERVLADIHP
                     GKPGAVELYFVGFAPDSSRDVFLREMRFVRRLFDERFGTAGRSIVLASSEAALEEFPI
                     GSATNLARTLARVGEAMNADEDLLFLFVTAHGSRDHRLSAVQPPLELAPLTPTALARM
                     LQDAGIKWRVLVISACYSGGFVEPLRDDNSIVITGAAPDRSSFGCEPGRDFTYFGQAY
                     FRDALAQTRSFTRAFELAKDLVAKQEAGEGLTPSLPQMWVGAGIRERLKQLADRPDQ"
     gene            complement(2829..>3208)
                     /locus_tag="VEL04_04770"
     CDS             complement(2829..>3208)
                     /locus_tag="VEL04_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47708.1"
                     /translation="FALLCWRLKKSQIEALCKRLRVPGEVRDLAVLASSNRDRMKSVR
                     TPRQLLDLLKRTDAFRRQERFAQLTEVAHLADPKINVALVGNARAAAAAVDASSLAAK
                     AASPAEIPKQLDAARIRAIAKAV"
BASE COUNT          520 a         1084 c         1062 g          542 t
ORIGIN      
        1 cgctcggctc gatcctcggg ggactcgcga cgccgacgga ggcggcgggc gttggcgcaa
       61 tcggcgcgct cggcctggcg gctgcgcacg gcaggctcga cgccgcgcgc ctgaagcacg
      121 cgctgctccg cacccttgcc acttccagca tggtgctttt cctcgcggtc gcgtcgaacg
      181 tcttcggcgc cgtgtttgcg cgcctcggca ccgccggctg gatcagcgag tggctgcttg
      241 ggctgagctt gccgccgatg ctgatgctgc tgctgatcgt ggccctcatc ttcctcctcg
      301 gctggccgtt cgagtggccg gcgatcatct tcgttttcgt gccgctgttc tacccggtgg
      361 tcgcgaagct gggcatcgac ctggtgtggt tcggcgcgct cgtcgcgctg acgctgcaga
      421 ccgcgttcct gtcgccgccg gtcgcgatgt cgggctattt cctcaagcag gtggccccgg
      481 cctggccgct gtcgaccatc ttcaagggca tgatggaatt catgggcatc caggtcatcg
      541 cagtggctct gctgctcgcg tttccggcga tcgcgacctg gctgcccgag cggctcaatc
      601 gtgccgcggc gccggggatc gagatcttcg acgtcgagcc gcgcccggac gacgcggggc
      661 gctaccaggc gatgatggta cggcgcgagc ctagctgacc gcggggaccg ccatcccttt
      721 ggccacggcg ggccgtgcgc cgatggcgtc gaaccagcgc ttcacgtgcg caaaggcgcc
      781 gagatcgacg cgatgccatt cgtggcgcgc cacccaggga taggtggcga tatccgcaat
      841 gctgtaatcg cttccagaca gaaaagcatt tttttccagt ctgcgatcca tgaccccata
      901 cagccgcttc gcctcgttgc cgtagcgctc gctgccgtac ggcacctcgt ccttcttcgc
      961 gcgcatgaag tggtgcgcct ggccgaacat cggcccgacc ccacccattt gaaacatcag
     1021 ccattgaaga atttgatatt tattcttttg agaaaaaaat tgacctgtct tgtcggcgag
     1081 gtagatcagg atcgcgcccg attccatcat cgcgatcggc ttgccgtccg ggccctgcgg
     1141 atcaacgatc gacgggatct tgccgttcgg attgatcttc aggtagtcgg gcgcgaactg
     1201 ctcgcccttg ctgatatcga tcttgtgcac gcggtacggc aggccgcact cctcgagcat
     1261 gatggaaacc ttgcggccgt tgggcgtgct ccaggtgtat aaatcgatca tggtgagagc
     1321 atgttgaaat ggctggctat tgtgtgcgtc gtgctggccg tggctgcgat catcgcgccg
     1381 cggcccgggc gtctcggcct cggccggctt cccggggact tccagatccc cgcccgcggg
     1441 cgaatctact acattcccct cgccagcacg ctgctgcttt cgctactggt ctgggcgatc
     1501 ggcaagctgc tttaggcgct cgcggatgcc cgcgcccacc cacatctggg gaagcgaggg
     1561 cgtgagcccc tctccagctt cctgcttcgc gaccaggtcc ttcgccagct cgaaggcgcg
     1621 cgtgaacgag cgcgtctgcg ccagggcgtc gcggaagtaa gcctggccga agtaggtgaa
     1681 gtcgcgcccc ggctcgcagc cgaaggagct gcggtcgggc gccgccccgg tgatcacgat
     1741 gctgttgtcg tcgcgcagcg gctcgacgaa tccgcccgaa tagcaggcgg agatgacgag
     1801 cacgcgccac ttgatgcccg catcctgcag catgcgcgcg agcgcggtcg gcgtaagcgg
     1861 cgcgagctcg agcggcggct gcaccgccga aagccggtga tcgcgggagc cgtgcgccgt
     1921 gacgaacagg aacagcaggt cctcgtcggc gttcatcgcc tcgccgacgc gcgcgagcgt
     1981 gcgcgcgagg ttggtcgcgc tgccgatcgg gaactcctcc agcgccgcct cgctgctcgc
     2041 aagcacgatc gagcggcccg cagtgccgaa gcgctcgtcg aacaggcgcc gcacgaagcg
     2101 catctcgcgc aggaacacgt cccggctcga gtcgggcgca aagccgacga aatacagctc
     2161 caccgcgccc ggcttgccgg gatggatgtc cgcgagcacg cgctcgatca gccgcccctg
     2221 caggtgaaac agccgctccc cggcgagcgg atcgggaccc gcctcctcct gcggcgcgcg
     2281 ccagacgtcg gtgcgcggaa aagcgaaaaa tccgatcgcg atcatggcgc tgaccgcagc
     2341 cgcgccgagg agaaactgcg gccgctgcgt cccgccgcac accgcaacgg cgcgcaccgc
     2401 gacgagccag gtccagccga ggagcaggta gaacgccgcc tccggatacc cgctcgccgc
     2461 ggcgaccgcg ggaagcgcga gcccggcgag ctcgtacacc gcgtcgctcg cgctgagcgc
     2521 ggtggcgatc agcagcagcc ggcccggctc gcggtacgcg agcgccgcga gcagcgcagc
     2581 ggcgagcgtc agcgacacct cggcgaaaaa aatcggcaac gccatggggt cgaactcgcc
     2641 cgcgaagccg gcgcgcaccg cggccgcggc gatccagaac gcaaagtcga accccatcag
     2701 caccgcgaag tgcaccggcg agacgcggta atcgaagggc cgcaccggca ggaacagcgc
     2761 gaggcgcgcc cccgcggcga ggttccttaa taaggaatga aaataaaaaa caaccgggcc
     2821 gcgctcggtc atacggcttt tgctatggcg cgaatgcgcg ccgcgtccag ctgcttcggg
     2881 atctcggccg gcgacgcggc cttggcggcg aggctgctgg catcgaccgc tgctgctgcc
     2941 gcccgcgcgt tgcctaccaa ggcgacgttg atcttgggat cggcgaggtg tgccacctcg
     3001 gtcagttgag cgaagcgctc ctgccggcga aacgcgtcag tccgcttcag caggtcgagc
     3061 agttgcctag gcgttctgac acttttcatg cgatcacggt tgctggaagc tagtacggct
     3121 aaatcgcgca cttcgcccgg gacgcgcaac cgcttgcaga gcgcttcgat ttgggacttc
     3181 ttcaggcgcc agcacagcag cgcaaagc
//
LOCUS       DASPXW010000202         3840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1446507, whole genome shotgun sequence.
ACCESSION   DASPXW010000202 DASPXW010000000
VERSION     DASPXW010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446507"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VEL04_04775"
     CDS             complement(<1..675)
                     /locus_tag="VEL04_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013698603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HYT47709.1"
                     /translation="MQRIRIVLCRPSHPGNIGAAARAMKTMGLSDLRLVAPQRYPAPE
                     AEWMATNAVDVLQAARLHSTLQESINDCVAAFALSARPREWSPQVLDVRSAAARALAS
                     GGKVAFVFGNESAGLTNEELFACQFLVHIPANPEFSSLNLAQAVQVVAYELFVASGAG
                     VPLAREEKAATVEDIEGLYAHLEQAAMASEFLQPGVNSRLRERWRRLFSRVPRLEREE
                     VNILRGL"
     gene            696..1472
                     /locus_tag="VEL04_04780"
     CDS             696..1472
                     /locus_tag="VEL04_04780"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HYT47710.1"
                     /translation="MHPLLNIAVRAARRAGSIINRAALDGSALKVRSKRANDFVTQID
                     SAAEEAIIGVVHKAYPDHGFLAEESGATPGDAEYVWIIDPLDGTTNFIHGFPQYCVSI
                     AVQHRGGMAHAVVYDPVKNELFTASKGRGAFLNDRRMRVSKCQKLDAALVGTGFPFKE
                     LSRLDLYTKQLRNLMQNSAGVRRAGAAALDLAYVASGRLDAFWELGLSAWDMAAGALL
                     IQEAGGLVGDLAGEQGFMESGDICAATPKVFPPLLEALAP"
     gene            complement(1506..2450)
                     /locus_tag="VEL04_04785"
     CDS             complement(1506..2450)
                     /locus_tag="VEL04_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYT47711.1"
                     /translation="MKRVLLVLLCAVFPAAGFPQTYPAKPVRLIVPFPPGGATDIIGR
                     LVAAKMQEVWGQPVVVENRPGAGTVVGTDLVAKSAPDGYTLGMVVTAHVINPSLRHNL
                     PYDTLHDLAAVTQVSVQQLVMAAHPSFPANNVAELIELAKRNPGKLSYATPGSGTAMH
                     LAVELLKSSTGIDIVHVPYKGGAPAQQDVMGGRVPILLDVLYAVTPLIDAGKIKVVAL
                     LGPERVSRYPVVADTVPGVSALSIVGIVVPSGLKNELIEKISNDIATAVKASDLTERM
                     KQLGMEPVGSTPEEFDALIRAEIEKWARVVKLSGAKAD"
     gene            complement(2453..3424)
                     /locus_tag="VEL04_04790"
     CDS             complement(2453..3424)
                     /locus_tag="VEL04_04790"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HYT47712.1"
                     /translation="MDTAAYQHASASFAWKADSFRNPRDYTIGCSPEELKRGYDEVRG
                     GRGFVVIRGLPLDGSLEDFIEAVRDVGSHFGHALSQNAQGERMTQVIDATHEDPTPRM
                     YRSNLELRPHSDITAMISLACWHKSQSGGESVIVSGVTVHDEMRRRAPQLLAPLYRGY
                     HYHRLGEEGPDEEPATPYRVPVFSSIKNQLSVRYQRSGIAAGHRERGEPLSDQDIAAF
                     NLFDEIAKAPGHRLSFFLERGEMIVLNNYTVMHARTQFTNYSEPERQRRLIRLWIDRE
                     GFREVPVEFRLFPSSNGVPQQTGKRATFDFKKLYADDPIATGGVPKL"
     gene            complement(3493..>3840)
                     /locus_tag="VEL04_04795"
     CDS             complement(3493..>3840)
                     /locus_tag="VEL04_04795"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYT47713.1"
                     /translation="APHLREGRLRALVTTLPHRSPLLAEVPSIVEAGFPRFPIASWAG
                     LFGPAGMPREVAERLNRELNALLKRPELRESLSRQAFEARGSSSDELAAYVKEQLETW
                     GRAIRGAGIQPEG"
BASE COUNT          630 a         1290 c         1279 g          641 t
ORIGIN      
        1 cagcccgcgc aggatgttga cctcctcgcg ctcgagtcgc ggcacgcgcg aaaacagcct
       61 gcgccagcgc tcgcgcaggc gcgagttcac gcccggctgg aggaattcgc tcgccatggc
      121 ggcctgctcc aaatgtgcat acaagccttc gatgtcttca accgtggcgg ctttctcctc
      181 gcgggcaagc ggcacgcctg cccccgacgc aacgaacagc tcgtaggcca ccacctgaac
      241 ggcttgcgcc agattgagcg aggagaactc gggattcgca ggaatatgca ccaggaactg
      301 gcaggcgaac agctcctcgt tcgtcagccc ggcgctctcg ttgccgaaca cgaacgccac
      361 cttgccgccc gacgccagtg cacgcgccgc cgccgagcgt acgtcgagca cctgcggcga
      421 ccattcgcgc ggccgggcgg aaagcgcgaa cgcggcgacg cagtcgttga tgctttcctg
      481 aagggtggaa tgaagcctgg ccgcctgaag aacgtcgacc gcgttcgtcg ccatccattc
      541 cgcctcgggc gcgggatagc gttgcggcgc gaccagccgc agatcggaga gccccatggt
      601 cttcatcgcg cgcgccgcgg cgccgatgtt gccggggtgg ctcggcctgc acaacacgat
      661 gcggatgcgc tgcatggctc tagaatacgg cgcccatgca tccgctgctc aacatcgcgg
      721 tgcgcgcagc gcggcgcgcc gggtcgatca tcaatcgcgc ggcgctcgac ggctcggcgc
      781 tcaaggtgcg ctccaagcgc gccaacgact tcgtcacgca gatcgacagc gcggccgagg
      841 aggcgatcat cggcgtcgtg cacaaggcgt accccgacca cggcttcctc gccgaggagt
      901 cgggcgcgac ccccggcgac gcggagtacg tgtggatcat cgatccgctc gacggcacga
      961 ccaatttcat ccacggcttc ccgcaatact gcgtctcgat cgccgtgcag caccgcggcg
     1021 gcatggcgca cgcggtggtg tacgacccgg tgaagaacga gctgttcacc gcgtcgaagg
     1081 gacgcggcgc gttcctcaat gaccggcgca tgcgcgtctc caaatgccag aagctcgacg
     1141 ctgcgctggt gggcaccggt ttcccgttca aggaattgtc gcgcctcgat ctctatacaa
     1201 aacagctcag aaatctcatg caaaactccg caggcgtgcg gcgcgccggc gccgccgcgc
     1261 tggatctggc ctacgtcgcc agcggacgcc tcgacgcctt ctgggagctg gggctctcgg
     1321 cctgggacat ggcggccggc gcgctgctca tccaggaagc cggcgggctg gtcggcgatc
     1381 tcgccggcga gcagggcttc atggaatccg gcgacatctg cgccgcgacg ccgaaagtat
     1441 tcccgccgct actcgaagcc ttggccccct gacaaggggg cggcgagccg caggcgagcg
     1501 gggggtcagt cggcctttgc acctgagagc ttcaccaccc gcgcccattt ctcgatttcg
     1561 gcgcggatca gcgcgtcgaa ctcctccggc gtcgatccga ccggctccat gcccagctgc
     1621 ttcatgcgct cggtaaggtc cgacgctttg accgcggttg cgatgtcatt gctgattttc
     1681 tcgattaatt cattcttcaa ccccgaagga acaacgatgc cgacaatgct cagcgcgctc
     1741 acgccgggga ccgtgtcggc gacgaccggg tagcgcgaga cgcgctccgg gccgagcagc
     1801 gccaccacct tgatcttgcc ggcgtcgatg agcggcgtga cggcgtacag cacatcgagc
     1861 aggatcggca cccggccgcc catcacgtcc tgctgcgcgg gcgcgccgcc cttgtacggc
     1921 acatgcacga tgtcgatgcc cgtcgagctc ttcagcagct cgacggcaag gtgcatcgcg
     1981 gtgccgcttc ccggcgtggc gtagctcagc ttgccggggt tgcgtttcgc cagctcgatc
     2041 agctcggcca cgttgttcgc cgggaacgag gggtgcgccg ccatcaccag ctgctgcacc
     2101 gacacctggg taaccgcggc aaggtcatgc agcgtgtcgt agggcaggtt gtgccgcagg
     2161 ctcggattga tgacgtgggc ggtcaccacc atcccgagcg tgtagccatc cggcgccgat
     2221 ttcgcgacca ggtcggtgcc aaccacggtg ccggcgccgg gccggttctc gaccaccacc
     2281 ggctgtcccc atacctcctg catcttggct gcgaccaggc ggccgatgat gtcggtggca
     2341 ccgcccggcg ggaagggaac gatcagccgg acgggttttg cggggtaggt ctgaggaaac
     2401 cccgcagcgg ggaaaacggc gcacagcagt accagcaata ctcgtttcat ccctacagct
     2461 tgggcacgcc gccggtggcg atggggtcgt ccgcgtagag cttcttgaag tcgaaagtcg
     2521 cgcgcttccc ggtttgctgc ggtacgccgt tgctggaagg aaacaggcga aactccaccg
     2581 gcacctcgcg aaagccctcg cgatcgatcc acaggcggat caggcggcgc tggcgctcgg
     2641 gctcggaata gttcgtgaac tgcgtgcgcg cgtgcatcac ggtgtagttg ttgagcacga
     2701 tcatctcgcc gcgctcgaga aagaagctga gccggtgccc gggcgccttg gcgatctcgt
     2761 cgaacaggtt gaaggcggcg atgtcctggt cgctcagcgg ctcgccgcgc tcgcggtgcc
     2821 ccgcggcgat gccgctgcgt tgataccgga cactcaattg atttttgata cttgaaaaaa
     2881 ccggcactct gtaaggcgtc gccggctctt catccgggcc ttcttcgccc aggcggtggt
     2941 agtggtagcc gcggtacagg ggcgcgagca gctgcggcgc gcgccggcgc atctcgtcgt
     3001 gcaccgtgac gccgctgacg atgaccgatt cgccccccga ctgcgacttg tgccagcagg
     3061 cgagcgagat catcgccgta atgtcgctgt gcggccgcag ctcgaggttg ctgcggtaca
     3121 tgcgcggcgt cgggtcctcg tgcgtcgcgt cgatgacctg cgtcatccgc tcgccctgcg
     3181 cgttctgcga cagggcgtgg ccgaaatggc tgccgacatc gcgcactgcc tcgatgaaat
     3241 cttcgagcga gccgtcgagc ggcaggccgc ggatcaccac gaatccgcgt ccgccgcgta
     3301 cctcgtcgta gccccgcttc agctcctccg gcgagcagcc gatcgtgtag tcgcgcgggt
     3361 tgcggaagga gtcggccttc cacgcaaacg aggccgaagc gtgctggtaa gcggcggtgt
     3421 ccatgcgaaa atcagctggt gattctacgc gcagcggcag cggtggcgag gtcgcgcgag
     3481 gtctggggct agtcagccct cgggctggat tccggcgccg cggattgccc tgccccaagt
     3541 ctcgagctgc tccttcacgt aggcggcaag ctcatcggaa gacgagcccc tggcttcaaa
     3601 agcctgcctg gagaggctct cgcgcagctc tggccgcttg agaagggcgt tcagctcgcg
     3661 gttgaggcgc tcggcgactt cccgcggcat cccggccggg ccgaacaggc ccgcccacga
     3721 cgcgatggga aatctcggga acccggcctc gacgatcgaa ggcacctcgg caaggagcgg
     3781 gctgcggtgc ggcagcgtgg tgacgagcgc gcgcagcctg ccttcgcgca gatgcggcgc
//
LOCUS       DASPXW010000203         2225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1697381, whole genome shotgun sequence.
ACCESSION   DASPXW010000203 DASPXW010000000
VERSION     DASPXW010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1697381"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..893)
                     /locus_tag="VEL04_04800"
     CDS             complement(51..893)
                     /locus_tag="VEL04_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HYT47714.1"
                     /translation="MAARAHRPQLTNAARPATFEVEAYNERGEMVPTSVAGEHPLTLY
                     LDKREIVTLMTLGHAPEALAIGYLRNQRLVNSIEDIAAVQVDWETNSAAVTTRNRKNI
                     DHKFKKRTVTSGCGQGTMFGDLMEEIDQVKLREDVRLTDANLFLLLEKVRKHETIYKQ
                     AGAVHGCALATLEGEILMFVEDVGRHNAVDAIAGFMWLDAIDGSDKLFYTTGRLTSEM
                     VIKCAQMRIPFLVSRSGLTQMGHEIAKKVGITMLGRASGRHYLAFTGKERLVRTLPQP
                     TALA"
     gene            complement(883..1956)
                     /gene="moaA"
                     /locus_tag="VEL04_04805"
     CDS             complement(883..1956)
                     /gene="moaA"
                     /locus_tag="VEL04_04805"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HYT47715.1"
                     /translation="MMKKVVGIRPESGAARPAAAAFAGGRLADTLGRPMRDLRISVTD
                     RCNFRCVYCMPREVFDANYRFLPHSAILSFEEIARLARIFVGLGVKKLRLTGGEPLVR
                     RNLERLVAMLSELKTEITLTTNGSLLARQADALKKAGLDRVTVSLDSLDDATFRAMND
                     ADFPVAKVIEGIEAAAAAGLAPVKINMVVKRGVNDGDIAAMAGRWRGSGHIVRFIEYM
                     DVGATNGWRMDDVVPSAEVVRRIGSRWPLEPIGANYPGEVAQRWRYADGAGEIGVISS
                     VTQAFCASCNRMRLSTEGSLYTCLFAGSGHDLKSLLRGGASDERMRDEIAALWQRRAD
                     RYSEIRTAETAKLRKVEMSYIGG"
     gene            2002..>2225
                     /locus_tag="VEL04_04810"
     CDS             2002..>2225
                     /locus_tag="VEL04_04810"
                     /inference="COORDINATES: protein motif:HMM:NF023177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3305 domain-containing protein"
                     /protein_id="HYT47716.1"
                     /translation="MEKPRFSVAVVMQRRASPSRWTDHVWEPWSVLPEYPPGAPRLLL
                     DEAGIAQWLHPGFTLVLHRDETEGYYMNVSA"
BASE COUNT          347 a          751 c          716 g          411 t
ORIGIN      
        1 ggcctgcacc gcgccgcctt cggctactac atcgtctcag cgcgcaaacc ctaggcgagc
       61 gccgtcggct gcggcagcgt cctgaccaga cgctctttcc cggtaaacgc caggtagtgc
      121 cggccgctgg cgcggcctag catggtgatg ccgacttttt tcgcgatctc atggcccatc
      181 tgcgtcaggc ccgagcgcga aacgaggaac gggatgcgca tctgcgcgca cttgatcacc
      241 atctcggagg tgaggcgccc ggtggtgtag aacagcttgt ccgagccgtc aatcgcgtca
      301 agccacatga atcccgcgat tgcgtcaacc gcattatggc gtcccacgtc ctcgacgaac
      361 atcaggatct cgccttccag ggtcgcgagc gcgcagccgt gcaccgcgcc cgcctgcttg
      421 tagatggtct cgtgcttgcg caccttctcg agcagcagaa acaggttggc gtcggtcagg
      481 cgcacgtcct cgcgcagctt gacctggtcg atctcttcca tcaggtcgcc gaacatggtt
      541 ccctggccgc agcccgaggt aacggttctt ttcttgaatt tatggtcgat gtttttcctg
      601 tttcgcgtcg tgacggcggc tgagttcgtc tcccagtcga cctgcaccgc cgcgatgtcc
      661 tcgatcgaat tcaccaggcg ctggttgcgc aggtagccga tcgccagcgc ctcgggcgcg
      721 tggccgagcg tcatcagggt gacgatctcg cgcttgtcga ggtagagcgt cagcggatgc
      781 tcgccggcca ccgaggtcgg caccatctcg ccgcgctcgt tataggcctc gacctcgaaa
      841 gtcgccgggc gcgcggcgtt ggtgagctgc gggcggtgcg ctctagccgc caatgtagga
      901 catctcgact ttgcgcagct tggcagtttc agcggtcctg atttcggaat agcggtcggc
      961 gcgccgctgc catagcgcgg cgatctcgtc gcgcatccgc tcgtcgctcg cgccgccgcg
     1021 cagcagcgac ttcaggtcgt ggcccgagcc ggcgaacaga caggtgtaga gcgagccctc
     1081 ggtcgaaagc cgcatgcggt tgcagctcgc gcagaacgcc tgcgttaccg aggagatgac
     1141 gccgatttcg cccgcgccgt cggcgtagcg ccagcgctgc gcgacctcgc ccggatagtt
     1201 ggcgccgatc ggctcgagcg gccagcggct gccgatgcgc cgcaccacct ccgccgaagg
     1261 caccacgtcg tccatgcgcc agccgttggt cgcgccgacg tccatgtact cgatgaagcg
     1321 cacgatgtgg ccgctgccgc gccagcgccc ggccatcgcg gcgatgtcgc cgtcgttgac
     1381 gccgcgcttc accaccatgt tgatcttcac cggcgcgagc ccggccgccg ccgcggcctc
     1441 gatgccctcg atcaccttcg ccacggggaa gtcggcgtcg ttcatggcgc ggaaggtcgc
     1501 atcgtcgagc gagtcgaggc tcaccgtgac gcggtccagc ccggcttttt tcagggcatc
     1561 agcttgcctg gcgagaagcg agccgttcgt cgtcagagtt atttcagttt tcagctccga
     1621 caacattgcc accaggcgct cgaggttcct gcgcaccagc ggctcgccgc cggtaagcct
     1681 gagcttcttc acgccgagcc cgacgaagat gcgcgccagc cgcgcgatct cctcgaagct
     1741 gaggatcgcg ctgtgcggca ggaagcggta gttcgcgtcg aacacttcgc gcggcatgca
     1801 gtacacgcag cggaagttgc agcggtcggt gaccgagatg cgcaggtcgc gcatcggccg
     1861 cccgagcgta tcggcgagcc ggccgccggc gaacgcggct gccgcgggac gcgccgcgcc
     1921 ggattccggc cgaatgccaa cgaccttctt catcatggtg ttttcccagg aaaatcatag
     1981 cagacggggt atagttgcgg cgtggaaaaa ccgcgatttt ccgtcgccgt ggtgatgcag
     2041 cgccgcgcct cgccaagccg ctggaccgat cacgtctggg agccctggag cgtgctgcct
     2101 gaatatccgc ccggtgcgcc gcgcttgctt ctcgacgagg cgggcatcgc gcaatggctg
     2161 catcccggct tcacgctggt gctgcatcgc gatgagaccg agggctacta catgaacgtc
     2221 tcggc
//
LOCUS       DASPXW010000204         2989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1823185, whole genome shotgun sequence.
ACCESSION   DASPXW010000204 DASPXW010000000
VERSION     DASPXW010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1823185"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..351)
                     /locus_tag="VEL04_04815"
     CDS             complement(67..351)
                     /locus_tag="VEL04_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HYT47717.1"
                     /translation="MAKKRKKAGKKRKPNAAFMKPMNPTGPLAAVVGSSAIPRTEVTK
                     KIWGYIKRNGLQDKKNKRMINADEKLRPVFGGKGQVSMFEMTKLVSRFLK"
     gene            521..1165
                     /gene="pdxH"
                     /locus_tag="VEL04_04820"
     CDS             521..1165
                     /gene="pdxH"
                     /locus_tag="VEL04_04820"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HYT47718.1"
                     /translation="MNIAELRQEYMRAGLAESRADPDPMRQFERWFEDALRARLPLPN
                     AMTLATVSAAGAPAARAVLLKGIESGGFVFHTNYRSRKGRELEARAAACLVFLWSELE
                     RQVRIEGGVEKVSPQDSDDYFATRPLGARLSAWASEQSEPVSSREILEKSMEQARQRY
                     GERPPRPAHWGGYRVVPQAIEFWQGRADRLHDRLLYTRKGGGVDGSWSIERLAP"
     gene            1155..2327
                     /locus_tag="VEL04_04825"
     CDS             1155..2327
                     /locus_tag="VEL04_04825"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYT47719.1"
                     /translation="MRPSDASQAREAILLLALAGFNAGISLRCVEPMLPKLATDFGTS
                     VSAASAIITSFALAYAGSVLLQGPLGDRFGKLRIVTAGTALAGAVSIGCAFAWDVGSL
                     AALRFTTGIFASSSVALGMAYIGDVVPLGERQTTVAHFIAGSLLGQTIGPLFGGALTD
                     MLGWRATFAVLGSVFLLVSLILFLRTAASWGPSVPGRFDPLAIHRALLSRPAVRWLAG
                     VGVAETFLFFGAYVFLSGYFKLRFDLSFTTIGLILAGYGVGGLLYSSTARWLVPLGER
                     GLVAAGGALGCALFLLVVLVPHWAYAIPCTVGLGLAFYLIHNTVQTRATEVAPDARGS
                     AVALYASAWAAGQALGVAAMGLAVSVFGYAPMIAVFGLGFGVLGLWLRSNLPRLRP"
     gene            2324..>2989
                     /locus_tag="VEL04_04830"
     CDS             2324..>2989
                     /locus_tag="VEL04_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HYT47720.1"
                     /translation="MSAAPPLDFVRRSGLARDGEQPKVEALSGGVSSDIWRVDLASGP
                     VCVKRALPRLRVAELWEAPIERNRYERRWMEIAARAAPGAAPAVLAGDDEAGLFAMQY
                     VDYPVWKERLRRGEADPAFAARVGATLADIHAATARREDIAREFPTDAIFYAIRLEPY
                     LVATGAKHPDLASIMKQLVHRTAKTRLALVHGDVSPKNILVGPHGPVFLDAECAWYGD
                     PAFD"
BASE COUNT          401 a         1043 c          992 g          553 t
ORIGIN      
        1 agttcctacc accagaaatg acaagaccgg ggaccgcccg gccttgtgca tttccgtgga
       61 aaacgcttac ttgagaaacc ggctgaccag cttggtcatc tcgaacatcg agacctggcc
      121 cttgccgccg aacaccggcc tcagcttctc gtcagcattg atcatgcgct tgtttttctt
      181 gtcctgaagg ccgttgcgct tgatgtagcc ccagatcttc ttggtcacct cggtgcgcgg
      241 aatcgcgctc gagccgacca ctgccgcgag cggcccggtc ggattcatcg gcttcatgaa
      301 ggccgcattc ggcttacgct tcttaccggc cttctttctc ttcttcgcca tctcggttct
      361 cctctgaacg tacgctacgt tgttcgatcc cggctttccc ccgccgcgga gcggcggtgg
      421 acgagaagat gccgaatccg caggtgcatg tcaacgactt tcagagggga aactcgacgt
      481 tttcactcag aagggttttt ttgagacgta aaatccctgc atgaacatcg ccgagctgcg
      541 ccaggagtac atgcgcgcgg gcctcgccga gtcgcgtgcc gaccccgatc cgatgcgcca
      601 gttcgagcgc tggttcgagg acgcgctgcg ggcgcggctg ccgctgccca acgccatgac
      661 gctcgcgacg gtgagcgccg cgggcgcgcc cgcggcgcgc gccgtgctgc tgaagggcat
      721 cgagagcggc ggcttcgtgt tccataccaa ttaccgcagc cgcaagggcc gcgagcttga
      781 agcgcgcgcc gccgcgtgcc tggtgttcct gtggtcggag ctcgagcgcc aggtgcgcat
      841 cgagggcggc gtggaaaagg tcagcccgca ggactccgac gactatttcg ccacccggcc
      901 gctcggcgcg cgcctctcgg cctgggcttc cgagcagagc gaacccgtat cttccaggga
      961 aattctcgaa aaatcgatgg aacaggcgcg ccagcgctac ggcgagcgcc cgccgcggcc
     1021 agcgcactgg ggcggctacc gcgtcgtgcc gcaggcgatc gaattctggc agggccgcgc
     1081 cgaccggctg cacgaccggc tgctgtacac acgcaaaggc ggcggcgtgg acggttcctg
     1141 gagcatcgag cggcttgcgc cctagcgacg cgtcgcaggc ccgcgaggcg atccttctac
     1201 tggcactggc cgggttcaac gccgggatct cgctgcgctg cgtggagccg atgctgccga
     1261 agctcgcgac cgacttcggc accagcgtct cggcggcctc ggcgatcatc acctcgttcg
     1321 cgctcgccta tgccgggtcg gtgctgctgc aggggccgct cggcgaccgc ttcggcaagc
     1381 tgcgcatcgt gacggccggc accgcgcttg ccggcgcagt ctcgatcggc tgcgcgttcg
     1441 cctgggacgt cggctcgctc gcggcgctgc gcttcaccac cggcattttc gcctcgtcct
     1501 cggtcgccct cggcatggcg tacatcggcg atgtggtccc gctcggcgag cgccagacca
     1561 cggtagcgca tttcatcgcc ggctcgctgc tcggccagac catcggaccg ctgttcggcg
     1621 gcgcgctgac cgacatgctg ggctggcgcg cgactttcgc cgtcctcggc tccgtgttcc
     1681 tgttggtttc attgatactt ttcctgcgca ccgcggcaag ctggggcccg tccgtgccgg
     1741 gacgcttcga cccgctcgcg atccaccgcg cgctgctgtc gcggccggcc gtgcgttggc
     1801 tcgccggcgt cggcgtcgcg gagaccttcc tgttcttcgg cgcctacgtc ttcctcagcg
     1861 ggtacttcaa gctgcgattc gacctctcgt tcaccaccat cggcctgatt ctcgccggct
     1921 acggcgtggg cgggctgctg tattcgtcga cggcgcggtg gctggtgccg ctgggcgagc
     1981 gcgggctggt cgctgccggc ggagcgctcg gctgcgcgct tttcctcctg gtggtgctgg
     2041 tgccgcactg ggcctacgcg atcccctgca ccgtcggcct gggtctcgcg ttctacctga
     2101 tccataacac cgtgcagact cgcgccacag aagtggcgcc cgacgcgcgc ggctcggcgg
     2161 tcgcgctgta cgcctcggcg tgggccgccg gccaggctct tggcgtcgcg gcgatgggcc
     2221 tcgcggtgag cgtcttcggc tacgcgccga tgatcgccgt gttcggcctg ggcttcggcg
     2281 tgctcggcct gtggctgcgc tcgaaccttc ccaggctgcg gccgtgagcg ctgcgccgcc
     2341 gctcgatttc gtgcgccgct ccgggctcgc gcgcgacggc gagcagccga aggtcgaggc
     2401 gctctccggc ggcgtgtcct ccgacatctg gcgcgtcgat ctcgccagcg ggccggtgtg
     2461 cgtgaagcgc gcgttgcccc gcctgcgcgt cgccgagctc tgggaagcgc cgatcgagcg
     2521 caaccgctac gagcgccgct ggatggaaat tgccgcgcgc gccgcccccg gggctgcgcc
     2581 ggcggtgctc gcgggggacg acgaagcggg cctgttcgcg atgcagtacg tcgactaccc
     2641 ggtgtggaaa gaacgcctgc gccgcggaga ggccgacccc gccttcgcgg cccgggtcgg
     2701 cgcaacgctg gcggacatcc atgctgccac ggcccgccgc gaggatatcg cgcgcgagtt
     2761 tcccaccgac gccatcttct atgccatccg gctcgagcct taccttgtgg caaccggagc
     2821 gaagcatcct gatctcgctt caataatgaa acagcttgtc catagaacag cgaaaacccg
     2881 gcttgccttg gtgcacggcg acgtcagccc gaagaacatc ctggtcgggc cgcacggccc
     2941 ggtgtttctc gacgccgaat gcgcctggta cggcgatccc gcgttcgac
//
LOCUS       DASPXW010000205         2360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_184938, whole genome shotgun sequence.
ACCESSION   DASPXW010000205 DASPXW010000000
VERSION     DASPXW010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184938"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..99
                     /locus_tag="VEL04_04835"
     CDS             4..99
                     /locus_tag="VEL04_04835"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HYT47721.1"
                     /translation="MGDYRIICEIRDEVLVVLALAVGHRREIYRR"
     gene            108..1190
                     /locus_tag="VEL04_04840"
     CDS             108..1190
                     /locus_tag="VEL04_04840"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HYT47722.1"
                     /translation="MTELAVFVALVTLGVAAWLVVRMQRLLEEKHRAMLTDLHDGLTK
                     QGDRLGGNLAELRESLGAKIDQRLDQISSRVSERLDEGFKKTNETFVSVMQRLATIDE
                     AQKKIETLTGSVVSLQELLGDKRSRGAFGEVQLEALVRNVLPPAAFEMQYTLSNGARV
                     DCALRLPDPTGLVCVDSKFPLENYKLMLDPKAAQAERALAQRAFRADLRRHIDAIAGK
                     YIIENETSDGAVMFIPAEAVFAEIHAYHSEIVDYANAKRVWIVSPTTLMAVLNTARAV
                     LKDVETRKQIHVIKEALGRLGQDFGRFEERMRRLADHIRQAHEDAEDVQVSSRKITQH
                     FQRIEAAELDQPPEQPAAKVVRLPEG"
     gene            1200..2171
                     /locus_tag="VEL04_04845"
     CDS             1200..2171
                     /locus_tag="VEL04_04845"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HYT47723.1"
                     /translation="MKRIAVLTSGGDAPGMNAAVRAVTRAALAEGWQVFGVRNGYAGL
                     LADTITPLLARDVGGIVQRGGTVLGSARCPEFVESPGRKAALASLRSHRIDALVVIGG
                     NGTQTGSAALAGEGYAVVGVPSTIDNDLYGTDVSIGADTAVNITLGAIDSLRTTASSH
                     QRAFAVETMGRECGYIALMAGIAGGAEVIALPESEIEPAEVAERLRAAYERGKTHAIV
                     VVAEGARCGIHELMGYYGAHRDSIGFELRVTRLGHVVRGGAPTAADRVLATRLGAAAV
                     DALARGQHGVLVGTVRNDLHATPLAEIAGRTRQADAALLELARTMAL"
BASE COUNT          408 a          808 c          812 g          332 t
ORIGIN      
        1 cgcgtggggg actaccggat catctgcgag atacgcgacg aagtgctggt ggttctggcg
       61 cttgccgtcg gccatcgtcg cgagatttac cggcgctaga atcgcgcatg accgagcttg
      121 ccgtgtttgt tgcgctcgtc accctcggag tcgcggcgtg gctggtggtg cgcatgcagc
      181 ggctgctcga ggaaaagcac cgcgccatgc tcaccgatct gcacgacggc ctgacgaagc
      241 agggcgatcg cctcggcggg aatctcgccg agctgcgcga gtcgctgggc gcgaaaatcg
      301 atcagcgcct cgaccagatc tccagccggg tgagcgagcg cctcgacgaa ggcttcaaga
      361 agaccaacga gaccttcgtc agcgtgatgc agcgcctcgc gaccatcgac gaggcgcaga
      421 agaagatcga gaccctgacc ggaagcgtgg tcagcctgca ggagctgctc ggcgacaagc
      481 gctcgcgcgg cgccttcggc gaggtgcagc tcgaggccct ggtgcgcaac gtgctgccgc
      541 cggcggcgtt cgagatgcag tacacgctgt cgaacggcgc gcgggtcgac tgcgcgctgc
      601 ggctgcccga ccctaccggc ttggtttgcg tcgactcgaa gttcccgctg gaaaattaca
      661 agctgatgct cgatccgaag gcagctcagg ccgagcgggc actggcgcag agggcgttcc
      721 gcgccgacct gcgccggcac atcgacgcca tcgccggcaa atacatcatc gagaacgaga
      781 cttcggacgg ggccgtgatg ttcattcccg ccgaagcggt attcgccgag atccacgcct
      841 atcactccga gatcgtcgac tacgccaacg cgaagcgcgt ctggatcgtg tcgccgacca
      901 cgctgatggc ggtgctcaac accgcgcgcg cggtgctgaa ggacgtcgag acgcgcaagc
      961 agatccacgt catcaaggag gcgcttgggc gcctgggcca ggacttcggc cgcttcgagg
     1021 agcgcatgcg caggctcgcc gaccatatcc gccaggcgca cgaggacgcc gaggacgtgc
     1081 aggtatcgag ccgcaagatc acccagcact tccagaggat cgaggcggcg gagctcgacc
     1141 agccgcccga gcagcccgcc gccaaggtcg tacgcctgcc cgagggctga aggctgggct
     1201 tgaagcgaat cgcggtcctc accagcggcg gcgacgcgcc cggcatgaat gccgccgtgc
     1261 gcgccgtgac gcgcgcggcg ctcgccgagg gctggcaggt gttcggcgtg cgcaacggct
     1321 acgccgggct gctcgccgac accatcacgc cgcttcttgc gcgcgacgtc ggcggcatcg
     1381 tgcagcgcgg cggcacggtg ctgggcagcg cgcgctgccc cgagttcgtc gagagcccgg
     1441 ggcgcaaggc tgcgctcgcc agcctgcgca gccaccgcat cgacgcgctg gtggtgatcg
     1501 gcggcaacgg cacgcagacc ggctcggcgg cgctggcggg cgagggctac gccgtggtgg
     1561 gcgtgccttc gaccatcgac aacgacctct acggcaccga cgtgagcatc ggcgcggaca
     1621 ccgcggtgaa catcacgctc ggcgcgatcg acagcctgcg caccacggct tcctcgcacc
     1681 agcgcgcctt cgccgtcgag acgatggggc gcgagtgcgg ctacatcgcc ctcatggccg
     1741 gcatcgccgg cggcgcggaa gtgatcgcgc tgcccgagag cgagatcgag cccgcggaag
     1801 tcgccgagcg gctgcgcgcc gcctacgagc gcggcaagac ccacgccatc gtggtcgtcg
     1861 ccgaaggcgc gcgctgcggc atccacgaat taatgggcta ttacggcgcg caccgcgact
     1921 cgatcggctt cgagctgcgc gtgacgcgcc tcggtcacgt ggtgcgcggc ggggctccga
     1981 ccgccgccga ccgcgtgctc gccacgcggc tcggcgccgc agcggtggac gcgctcgcgc
     2041 gcgggcaaca cggcgtgctg gtcggaactg tgcgcaacga cttgcacgcc acgccgctcg
     2101 ccgagatcgc cggacgcacg cggcaggcgg acgccgcgct gcttgaactc gcgcgcacga
     2161 tggcgctcta agttggtcac gacaggtaga cctgccgctt ccccgctact tatcttcgcg
     2221 tcgagaaaac tcggcccaaa gtcccacggc ggcgacgaca aacggaagga tcagcaggaa
     2281 caggaatgat ctgtccaggc ccgagaacca ctgcacccag agaccccagt cgaatatctc
     2341 cgatacctga cccttgaacc
//
LOCUS       DASPXW010000206         2659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1698310, whole genome shotgun sequence.
ACCESSION   DASPXW010000206 DASPXW010000000
VERSION     DASPXW010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1698310"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..1584
                     /locus_tag="VEL04_04850"
     CDS             109..1584
                     /locus_tag="VEL04_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYT47724.1"
                     /translation="MTTVFSAFEQTARAHPAKTFLHVYPESIRLSYGEALERIAGLAA
                     RYRARRYGAGHRVALRLENCPEFLLHFLALNAVGASVVPLNPDYRPAELEYVLGHSEA
                     GLLIDSSFLKGDFDPPPAGKPCGADECALLYTSGTTGKPKGCLLSNFYFLNVGKRYLA
                     EGGLCGVRFGEERLITPLPLFHMNALAVSTVAMILAAGCVVQLDRFHPKTWWRDVAAS
                     GATIVHYLGVMPAVLLKLAETSNDGGRRVRFGYGANANPQDHAAFEERFGFPLIEAWA
                     MTETGGGALIAASREPRHVGTRCIGIPKEDLEIKILKETGELLVRQAGADPRRGFFSG
                     YLKDAAATEEAWRGGWFHTGDAVRRGAEGMLHFVDRLKNIIRRAGENIAALEVEAALA
                     GHPAIAQVAVIAAPDPVRDEEVMAVIVPHDRVSLTKETAISIQDWCLERLAYFKAPGY
                     VVFRDSLPVTSTNKVQKAKLADFSFEPRDRFDLRDRKRRKS"
     gene            1581..>2659
                     /locus_tag="VEL04_04855"
     CDS             1581..>2659
                     /locus_tag="VEL04_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HYT47725.1"
                     /translation="MRQGYEGVALAAPVTVPYVRYSIRAAHWWLATALSQILRTSALN
                     KEQVDGLTVSSFTLAPDTAIGLTQHLGLAPRWLDHIPLGGASGVVALRRAARAVQSGD
                     AEVVACLAGDTNHVDSFRLTLANFSQFARDAVYPYGAGGPNASFAFLTQYYMRTYGAR
                     REDFGKLCVAQRANALKFPFALFKKPLTLDEYLAARPIAEPLHLFDCVMPCAGAEGFL
                     VLSVEKAETLNLPYARLLGTIERHNAFASDPVQMRAGWALDRDALYAQAGVGPAELDF
                     VETYDDYPVISLMQLEDLGFCAKGEGADFVRKHTFTCDGSFPLNTSGGQLSVGQAGAA
                     GGFLGLVEAIRQLTGEAGTRQVKDA"
BASE COUNT          441 a          904 c          823 g          491 t
ORIGIN      
        1 tcgcaagcac gaacggctgg atttttctca ccgcaaccct cctcccgaag tccgcgaagt
       61 ggtccttgag gttgtcctat tggcgcgcgg catcttacca tcctgagagt gaccacggtc
      121 ttttccgcct tcgagcaaac cgcccgcgca catcccgcca agacttttct tcacgtttac
      181 cctgaaagca ttcgcctgag ctacggcgag gcgctcgagc gcatcgccgg cctcgcggca
      241 cgctaccgcg cgcgccgcta cggcgccggc caccgcgtcg ccctgcggct cgagaactgc
      301 ccggaattcc tgctgcactt cctggcgctc aacgcggtcg gcgcgagcgt ggtaccgctc
      361 aatcccgact accggccggc cgagctcgag tatgtgctcg ggcatagcga agccggtctt
      421 ctcatagaca gttctttcct gaaaggcgat ttcgatccgc cgcccgccgg caagccctgc
      481 ggcgccgacg aatgcgccct gctctatacc tcgggcacca ccggcaagcc gaaaggctgc
      541 ctgctcagca acttctactt cctgaatgtc ggcaagcgct atctcgccga gggcggcttg
      601 tgcggcgtgc gcttcggcga ggagcggctc atcacgccgc tgccgctgtt tcacatgaac
      661 gcgctcgccg tctcgacggt cgcgatgatc ctcgccgcgg gctgcgtggt gcagctcgac
      721 cgtttccatc cgaagacctg gtggcgcgac gtcgcagcat cgggcgcgac gatcgtgcac
      781 tacctcggcg tcatgcccgc cgttctcctg aagcttgcgg aaacttcgaa cgacggaggc
      841 cgtcgcgtgc gcttcggcta tggcgccaac gccaatccgc aggatcacgc cgcgttcgag
      901 gagcgcttcg gctttccgct catcgaggca tgggcgatga cggagaccgg cggcggcgcc
      961 ctgatcgccg cgagccgcga gccgcgtcat gtaggtacgc ggtgtattgg aattccaaaa
     1021 gaagatcttg aaattaaaat cctgaaggaa accggcgagc tgctggtgcg ccaagccggt
     1081 gccgatccgc ggcgcggatt cttctccggc tacctcaagg acgccgcggc caccgaggag
     1141 gcctggcgcg gcggctggtt ccataccggc gatgccgtgc gccgcggtgc ggagggcatg
     1201 ctgcatttcg tcgaccggct caagaacatc atccgacgcg ccggcgagaa catcgccgcg
     1261 ctcgaggtcg aagccgcact cgccggacac ccggcgatcg cgcaagttgc ggtcatcgct
     1321 gcgcccgacc cggtgcgcga tgaggaggtg atggccgtca tcgttcccca tgacagagtt
     1381 tctttgacaa aggaaaccgc catctccatc caggactggt gcctcgagcg gctcgcgtat
     1441 ttcaaggcgc cgggctacgt cgtgttccgc gactcgcttc ccgtcacctc gaccaacaag
     1501 gtgcagaagg cgaagctcgc cgatttctcc ttcgagccgc gggatcgctt cgatctgcgt
     1561 gaccggaaaa gacgtaaatc atgagacaag gctacgaagg cgtggcgctc gcggctccgg
     1621 tgacggtgcc ctatgtgcgc tactcgatcc gcgcggcgca ttggtggctc gcaaccgccc
     1681 tgtcgcaaat tctcaggact tcggccctca acaaagagca ggtcgacggc ctcaccgtct
     1741 cgagcttcac gctcgcgccg gataccgcca ttggcctcac gcagcatctc gggctcgcgc
     1801 cgcgctggct cgaccacatt ccgctcggcg gcgcgagcgg tgtggtggcg ctgcggcgcg
     1861 ccgcgcgcgc ggtccagtcg ggcgacgccg aggtcgtggc ctgcctcgcc ggcgacacca
     1921 accatgtcga ctcgttccgg ctcacgctcg ccaacttcag ccagttcgcg cgcgacgcgg
     1981 tgtatccgta cggtgccggc ggtccgaacg cgagcttcgc tttcctcacg cagtactaca
     2041 tgcgcaccta cggcgccaga cgcgaggatt tcggcaagct gtgcgtcgca cagcgcgcca
     2101 atgctctcaa gtttcctttt gccttattca aaaaaccttt gaccctggac gagtacctgg
     2161 cggcgcggcc gatcgccgag ccgctgcatc ttttcgattg cgtcatgcct tgtgccggag
     2221 cggagggctt tctggttctt tcggttgaaa aagccgagac gttgaacctc ccctatgccc
     2281 gccttctcgg caccatagag aggcacaatg ccttcgcttc cgatccggta cagatgcgcg
     2341 ccggctgggc gctcgaccgc gacgcgctgt acgcgcaggc gggagtcggg cccgccgagc
     2401 tcgatttcgt cgaaacctat gacgattacc cggtaatctc gctgatgcag ctcgaggacc
     2461 tcggcttctg cgccaagggc gaaggtgcgg atttcgtgcg caagcatact tttacctgcg
     2521 acggctcgtt ccctctcaac accagcggcg gacagctctc ggtcgggcag gccggcgccg
     2581 cgggcggctt cctcggcctg gtcgaggcga tcaggcagct caccggcgag gcgggcacgc
     2641 gccaggtgaa ggatgcgaa
//
LOCUS       DASPXW010000207         3098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1324115, whole genome shotgun sequence.
ACCESSION   DASPXW010000207 DASPXW010000000
VERSION     DASPXW010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324115"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VEL04_04860"
     CDS             <1..555
                     /locus_tag="VEL04_04860"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYT47726.1"
                     /translation="KVLKERNIPKASVVGHSMGSLVAIEFAARHPQAAERIALISAAH
                     PMKVSDAFLDAAKRNDYSAFDMHTIWGHAAQVPLGGNPNPGMWMYGDTQARLERLAPG
                     VLHNDLKACNDYVFSGPVTCPALFVLGKRDVMTPPKSALRLAEAIPHSTTVIVNFSGH
                     SLMAEAPDATLDALIEFFSQGADQ"
     gene            552..1517
                     /locus_tag="VEL04_04865"
     CDS             552..1517
                     /locus_tag="VEL04_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYT47727.1"
                     /translation="MSRFAKWLCVLAALLPALGGAQPYPVKPVKIVVPAQPGGGLDLV
                     GRTIADQLGRAMNQSFIVENSGGGGGAIASQAVARAAPDGYTLMVGYVGTHGTNPAVR
                     KLPYDAIRDFTAIAMVGGTRNVLVVHPSVPAADLRSFVDYARNNAAKLNYATGGIGLL
                     NHLALEQFRAAAGFDAVYAHYRGIGPAIADMLGGQIQVLMPGLAAALPHIRAGKIKPL
                     AVTGLERHPLIPGVPTFEESGFKGFDGVQWYGIVGPAKLPTEITQRLNAEINKALASP
                     ALRQRLSGEAIDPMPMTPEQFAGFIRADIARWSALARERGISLDD"
     gene            complement(1527..2876)
                     /locus_tag="VEL04_04870"
     CDS             complement(1527..2876)
                     /locus_tag="VEL04_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HYT47728.1"
                     /translation="MTESVSEQIAAAVERARRIPPQVRDVVEKLLIDVAGLCVAARNT
                     DYTRSALAGWEGNGSCTAIGHARTLDAAGAAFVNGTSAHGEDFDDTFEGGPVHAGAVI
                     VPAILAIAERNSIEGSDALRGIAVGVEVMCRASLVAPKLIHKAGFHPTAVLGAMGAAA
                     GVAAALHLSRNEFVHALGIAGSMASGIIEYLAEGAWTKRMHPGWAAQSGIRAADLARA
                     GFVGPRTVFEGTHGLYYGFARTAGGDWAKLLDGFGSQWLVASIAFKPYACGTMTQPYV
                     DCARRLAKKIDVENIKEIICEAAEGTVHRLWEPLAAKQRPPNAYAAKFSQPYCIAAGF
                     VLGHAGLDAFTEERVRDERLRGLAAKVRYEIDPHNPYPDEFTGHVRAKLRDGSVVEER
                     QPHLRGGRSEPLSRADIEEKFRLNCAYGGWSAAQAERFLAFARQAFDSRIDLSPFRS"
     gene            complement(2869..>3098)
                     /locus_tag="VEL04_04875"
     CDS             complement(2869..>3098)
                     /locus_tag="VEL04_04875"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HYT47729.1"
                     /translation="AYGGLTFWFCQLYPRARTRACYAAGFIALGVGMEFLQDMTDYRT
                     FEVLDMAANTIGIFLGWAAALILPVAWLRHD"
BASE COUNT          510 a         1064 c         1020 g          504 t
ORIGIN      
        1 aaagtcctaa aagaaagaaa tatcccaaag gcgagtgtcg tcggccactc gatgggctcg
       61 ctcgtcgcca tcgaattcgc cgcccgccat ccgcaagccg ccgagcgcat cgcgctgatc
      121 agcgccgccc atccgatgaa agtcagcgac gcgtttctcg atgcggcgaa aaggaacgac
      181 tactcggcgt tcgacatgca caccatctgg ggccatgctg cgcaggtgcc gctcggcggc
      241 aaccccaacc ccggcatgtg gatgtatggc gacacgcagg cgcgcctcga gcgcctcgcc
      301 cccggcgtcc tccacaacga cctcaaggcc tgcaacgact atgtgttttc gggcccggtg
      361 acatgtccgg cgctgttcgt tctcggcaag cgcgacgtga tgaccccccc gaaaagcgcg
      421 ctgcgcctcg ccgaagcgat cccccattcc accaccgtca tcgtcaactt ctccggccat
      481 tcgctgatgg ccgaggcgcc cgatgcgacg ctcgacgcgc tgatagaatt tttttcgcaa
      541 ggggctgacc agtgagtcga tttgcgaaat ggctctgcgt cctggccgcg ctgctgccgg
      601 cgctcggcgg tgcgcagccc tatccggtca agcccgtgaa gatcgtggtt cccgcgcagc
      661 cgggcggcgg cctggatctg gtcggccgca cgatcgcgga ccagctcggc cgcgcgatga
      721 atcagtcctt catcgtcgag aattcaggag gcggcggcgg agccatcgcc tcgcaggcgg
      781 tcgcgcgcgc tgcgcccgac ggctatacgc tgatggtggg ctacgtcggc acgcacggca
      841 ccaatccggc cgtgcgcaag ctgccgtacg acgccatccg ggacttcacc gccatcgcca
      901 tggtcggcgg cacccgcaat gtcctggtcg tgcatccctc ggtgccggcc gccgatctga
      961 ggagcttcgt cgactatgcg aggaacaacg ccgccaagct gaactacgcg acgggcggga
     1021 tcggcttgct caaccacctc gccctggagc agttcagggc cgccgcgggc ttcgacgccg
     1081 tgtacgcgca ctaccgcggg attggtcccg cgattgccga catgctcggc gggcagatcc
     1141 aggtgctgat gccgggcctc gccgcggcgc tgccgcacat ccgcgcaggc aaaataaagc
     1201 cgctcgccgt caccggcctc gagcgccacc cgctgattcc cggcgtgcct accttcgagg
     1261 aatcggggtt caagggcttc gacggcgtgc agtggtacgg catcgtcggg ccggcgaagc
     1321 tgccgacgga gatcacgcag cggctcaacg ccgagatcaa caaggcgctc gcctccccgg
     1381 cgctgcggca gcgcctttcg ggggaagcga tcgatccgat gccgatgacg cccgagcagt
     1441 tcgcaggctt catccgggcc gacatcgcgc gctggagcgc gctcgcgcgc gagcgcggga
     1501 tttcgctcga cgactagccg agccgctcac gagcggaacg gcgacaggtc gatgcgcgag
     1561 tcgaaggcct gtcgcgcaaa agcgaggaaa cgttccgcct gcgccgcgct ccagccgccg
     1621 taggcgcagt tcagcctgaa cttctcctcg atgtcggcgc gcgacagcgg ctcgctgcgg
     1681 ccaccgcgca ggtgcggctg gcgctcctcg accacgctgc catcgcgcag cttcgcgcgc
     1741 acatgaccgg tgaattcgtc ggggtagggg ttgtgcggat cgatctcgta gcgcactttt
     1801 gccgcgaggc cgcgcaggcg ctcgtcgcgc acgcgctcct cggtgaaggc gtcgaggccg
     1861 gcgtgcccga gcacgaagcc ggcggcgatg cagtagggct ggctgaactt ggcggcgtag
     1921 gcgttgggcg ggcgctgctt cgccgccagc ggttcccata gacgatgaac cgtcccttcc
     1981 gcagcttcac agatgatttc ttttatgttc tcaacatcaa tttttttcgc caaccgtcga
     2041 gcgcaatcga cgtagggctg cgtcatggtg ccgcaggcat agggcttgaa cgcgatggat
     2101 gccaccagcc actggctgcc gaagccgtcg aggagctttg cccagtcgcc cccggcggtg
     2161 cgcgcgaagc cgtagtagag cccgtgcgtg ccttcgaaca ccgtgcgcgg cccgacgaag
     2221 ccggcgcgcg ccaggtcggc ggcgcgaatt cccgattgcg ccgcccagcc cggatgcatg
     2281 cgcttggtcc acgcgccctc cgccaggtac tcgatgatgc ccgaggccat cgagcccgct
     2341 atgcccagcg catggacgaa ctcattcctc gagaggtgca aagctgcggc cacgcccgcg
     2401 gcagcgccca tagcgccgag caccgcggtg gggtgaaagc ccgccttgtg gatgagcttc
     2461 ggggcgacca ggctggcgcg gcacatcacc tcgacgccga ccgcgatgcc gcgcagggca
     2521 tcgcttcctt cgatgctgtt tctttctgca atggcgagaa tcgcgggcac gattaccgcg
     2581 ccggcgtgca ctggcccgcc ctcgaaggtg tcgtcgaaat cctcgccgtg cgcgctggtg
     2641 ccgttgacga aggcggcgcc tgcagcatcg agcgtgcgcg cgtggccgat tgccgtgcag
     2701 ctgccgttgc cctcccagcc cgcgagcgcg ctgcgcgtgt aatcggtgtt gcgcgccgcg
     2761 acgcagagac cggccacgtc gatcagcagc ttctcgacga cgtcgcgcac ctggggcggt
     2821 atccggcggg cgcgctcgac cgcggcggcg atctgttcgg ataccgattc agtcatggcg
     2881 cagccaggcg acgggcagga tgagcgcggc cgcccagccg agaaatatgc cgatcgtgtt
     2941 cgcggccatg tcgagcactt cgaaagtgcg atagtcggtc atgtcctgaa ggaattccat
     3001 gccgacgccc aaggcgatga acccggctgc gtagcacgcc ctggtccgcg ctctcgggta
     3061 aagctggcag aaccagaacg tcaggccgcc ataggcga
//
LOCUS       DASPXW010000208         7376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1197570, whole genome shotgun sequence.
ACCESSION   DASPXW010000208 DASPXW010000000
VERSION     DASPXW010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7376
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197570"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2101
                     /locus_tag="VEL04_04880"
     CDS             <1..2101
                     /locus_tag="VEL04_04880"
                     /inference="COORDINATES: protein motif:HMM:NF017857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HYT47730.1"
                     /translation="IPKVDEPRPYTRINPRPTEEQWQASGEHRARLARALVEQDFRYV
                     SLGYQYGRLEASLTNVRISSMPRAVGRAARTLLSFAPLETREIRITYLESGLPIATYT
                     FINMPLLQRYFNGLAQRQALAPYVAIEYANPAAQREEADRAETLEAFRDALPQSIVLE
                     TQGADFFALRGANIAGGTFLVRPGFSGFFNDPSGAFRFDLSLVENYDRPLGDNLFFSA
                     ETRLPLYEDISAVTQPSNSTLPHVRTDIAEYRRHAKFRLTRLMLNKYTHVSERVYARA
                     SAGIYEEMFSGFGGQMLYLGRDGRWGVDLDVNWLKQRDFDGWFGHQRYETVTAIASLN
                     YRMAHDFTATLRAGRFLARDKGVRAEIKRRFKSGWEVGAWYTVTNGNDITSPGSPESP
                     YYDKGIFIVWRLDTLLTRDTRATAGFAISPWTRDVGQMVASPGDLYSIFEDPVFRMHE
                     RDGLSRLADRNDDYDLPSPGTGRDRVWPDFIGEDLFSDLPAAQCRVLDPELQGRYVGA
                     CVNGLAEGRGSASGYARYEGEFKAGRKHGKGVKTWPNGDRYEGDFVEDRKEGSASYTW
                     GRGSWMGERYEGAYLADRRHGFGVYRWPSGDVYSGPWKDDAFAGQPTEMMIARAIFEK
                     EAAAAVAKEGQKVCREMTVGVGGRDWVRGVVVATSAEKVGVRIEEPGEFPHVIAEVEA
                     RKGDVVWDAPTNWTPCW"
     gene            2183..2494
                     /locus_tag="VEL04_04885"
     CDS             2183..2494
                     /locus_tag="VEL04_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47731.1"
                     /translation="MPLPEWLHDLPFAWCVVVAIERPDALRAAAAATAAAPMLIAGTF
                     TGAPPAAEPTPAPDAAPPAAPAPPAVPAAPPAPCDKATLEESNTDRMTASLLNVSIKT
                     S"
     gene            complement(2590..3495)
                     /locus_tag="VEL04_04890"
     CDS             complement(2590..3495)
                     /locus_tag="VEL04_04890"
                     /inference="COORDINATES: protein motif:HMM:NF021472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HYT47732.1"
                     /translation="MAILTLLSGGAAAYNGAYVASRAAIGEKTKEVKAGFVMAEEKSF
                     RDRQVRSSEIRIRKIGVKDLWQALKEGFDDFNAKPSVIAFLIVIYPLFALLWTLLLVG
                     DNLLHLAFPMVAGFTFLGPVVSVGLFEMSRRRERGLDLTWRSAFDFVHSSSFAPILAL
                     SVTMMLLYVAWLYAAETLYFGLFGAVSPDSISDFVTQVLTTRRGGALIFYGTVLGFFF
                     AVAALAISVVSFPLALDRPVTAITAAHASIKAVSSNTLMMALWGLIVVVLLAAGAILF
                     LIGLAVVLPVLGHATWHLYRKLVEP"
     gene            complement(3544..6240)
                     /gene="polA"
                     /locus_tag="VEL04_04895"
     CDS             complement(3544..6240)
                     /gene="polA"
                     /locus_tag="VEL04_04895"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HYT47733.1"
                     /translation="MLVDGSSYLYRAFHALPELKSPKGEPTGAIYGVLSMLRKLGADY
                     KAQARACVFDAKGKTFRDAQYPAYKANRASMPDDLGAQVEPLREAIEALGWPVLTIDG
                     VEADDVIATLVELAKRRGWRSIISTGDKDLTQLVDEHVTWVNTMSNEILDIEGVRKKF
                     GVPPDRIVDYLTLVGDAIDNIPGVEGVGPGFASKWLSQYGSLDNLVANASQIPGVRGE
                     NLRKKLDWIPKARELVTVRRDVPLPVTLDQLAEGKPDAARQRTLYERFGFRTWLRETE
                     SAAPAPSQEIPLVAEERTPGPEKRKYSAILTEDELRALVKQLEAAPLVGFDTECVGLE
                     PMTARLVGMSFALGRQAFYLPLSHEYPGAPAQLPMDLALQTLKPWLERDARGKVGQNV
                     KFDSHVLANQGIRLAGCVHDTLLESYVLEVHERHDLGVLAQRHCGWQTLSYDDVTGKG
                     AARIEFSAVEIGRATQYAAEDADCTLAVHDVLYPKIKQDEKLSFVYEKIEMPVLPVLF
                     RMERNGVLLDAAKLEAQSHELGKEVLKKERQAHEAAGQPFNLGSPKQIQEILFDKHQL
                     PVKKKTPSGQPSTDEDVLEELALDHPLPRLILEHRALAKLKSTYTDKLPKSVHPGTGR
                     VHTTYSQTTAVTGRLASNDPNLQNIPIRTAAGRRIRECFIAPAGAKIVSADYSQIELR
                     IMAHLSGDESLKQAFARGHDVHRATAAEVFGVPLDQVSNDERRAAKVINFGLIYGMSS
                     FGVAQNLGIERVTAQAYIDSYFSRYPGVKRFMDRTRELARTRGYVETVFGRRLYLPEM
                     KSGSPQRRQAAERAAINAPMQGTAADLIKLAMISVQKWLDESRLKTLLIMQVHDELVL
                     EVPEDELTVAKEKVRELMQSVAKLDVPLIVDAGVGDNWDQAH"
     gene            6398..6724
                     /locus_tag="VEL04_04900"
     CDS             6398..6724
                     /locus_tag="VEL04_04900"
                     /inference="COORDINATES: protein motif:HMM:NF022639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2782 domain-containing protein"
                     /protein_id="HYT47734.1"
                     /translation="MRRLLVLVMFFSLSVMAQQRPPKLEPLPEPPPPPPIPGPDEPAV
                     RIPVQEADKVEEIREGGRVVMLKVTPAGGKPYYLVDTTGNGNWMRRDSLDDGVRVPMW
                     PIHIFD"
     gene            6746..>7376
                     /locus_tag="VEL04_04905"
     CDS             6746..>7376
                     /locus_tag="VEL04_04905"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HYT47735.1"
                     /translation="MSVYTPVSEAQLADWLRNYSVGTLARCEPIKAGIENTNYFVTTT
                     QGRYVLTLFERLPADELPFYLGLMAHLARHGIPCPAPIADLSDQYLQRLNGKPAALVT
                     RLPGRSLENPGASHCAELGALLARMHLAGRSYGAYLENPRGAKWWRFAARAVAPFLDP
                     ENRSLLENEIAFQAEHRLPDLPRGPVHADLFRDNALFEDARISGVIDFYL"
BASE COUNT         1281 a         2426 c         2362 g         1307 t
ORIGIN      
        1 cattccgaag gtcgacgagc cgcggcctta cacgcgcatc aacccgcgcc cgaccgagga
       61 gcagtggcag gcctcgggcg agcatcgcgc gcgcctcgcg cgcgcgctgg tagagcagga
      121 cttccgctac gtgagcctcg gctaccagta cggcaggctc gaggcgagcc tcaccaacgt
      181 gcgcatctcg tcgatgccgc gcgcggtcgg gcgcgccgcg cgcaccctgc tttccttcgc
      241 gccgctcgag acgcgcgaga tccgcatcac ctacctcgag agcgggctgc cgatcgcgac
      301 ctacaccttc atcaacatgc cgctgcttca gcgctacttc aacggccttg cgcagcgcca
      361 ggcgctcgcg ccttacgtcg ccatcgagta cgccaacccg gcggcgcagc gcgaggaagc
      421 cgaccgcgcc gagacgctcg aagcgttccg ggacgcgctg ccgcagagca tcgtgctcga
      481 gacgcagggc gccgacttct tcgcgctgcg cggcgcgaac atcgccggcg gcaccttcct
      541 cgtgcgcccc ggcttctccg gcttcttcaa cgacccgagc ggcgccttcc gcttcgacct
      601 atcgctcgtg gagaactacg accgtccgct cggcgacaat ctctttttct cggccgagac
      661 gcgcctgccg ctctacgagg acatcagcgc ggtcacgcag ccctcgaaca gcacgctgcc
      721 gcacgtgcgc accgacatcg ccgagtaccg gcggcatgcc aagttccgcc tcacgcgcct
      781 gatgctcaac aagtacaccc acgtctcgga gcgcgtctac gctcgcgcct cggccggcat
      841 ctacgaggag atgttcagcg gcttcggcgg ccagatgctc tacctcgggc gcgacgggcg
      901 ctggggcgtc gatctcgacg tcaactggct caagcagcgc gacttcgacg gctggttcgg
      961 ccaccagcgc tacgagaccg tcaccgccat cgcctcgctc aattaccgca tggcgcacga
     1021 cttcaccgcg acgctgcgcg ccgggcgctt tctcgcgcgc gacaagggcg tgcgcgccga
     1081 gatcaagcgg cgcttcaagt cgggctggga agtcggcgcc tggtacaccg tgaccaacgg
     1141 caacgacatc acctcgcccg gctcacccga gagcccgtac tacgacaagg gcatcttcat
     1201 cgtgtggcgc ttggacacgc tgctcacgcg cgacacccgc gccaccgccg gcttcgccat
     1261 ctcgccctgg acgcgcgacg tcggccagat ggtcgcctcg cccggcgatc tctacagcat
     1321 tttcgaggat ccggtgttcc gcatgcacga gcgcgacggg ctttcgcgcc tcgccgaccg
     1381 caacgacgac tacgacctgc cgtccccggg caccgggcgc gatcgcgtct ggccggattt
     1441 catcggcgag gatctcttca gcgacctgcc cgccgcccag tgccgcgtgc tcgatccgga
     1501 gctgcaggga cgctacgtgg gcgcgtgcgt caatgggctc gccgaggggc gcgggagcgc
     1561 gagcggctac gcgcgctacg agggcgagtt caaggccggg cgcaagcacg gcaagggcgt
     1621 gaagacctgg ccgaacggcg atcgctacga gggcgatttt gtcgaggacc gcaaggaagg
     1681 ctcagccagc tacacctggg gacgcggatc gtggatgggc gagcgctacg agggcgccta
     1741 tctggcggac cgccgccacg gcttcggcgt ctaccgctgg ccgagcggcg acgtttactc
     1801 tggaccgtgg aaggacgacg ccttcgccgg gcagccgacc gagatgatga tcgcgcgcgc
     1861 catcttcgag aaggaggccg ctgccgcggt ggcgaaggaa ggccagaagg tgtgccgcga
     1921 aatgaccgtc ggcgtcggcg gccgcgactg ggtgcgcggc gtggtcgtgg cgacgagcgc
     1981 cgagaaggtc ggcgtgcgga tagaggagcc cggcgaattt ccgcacgtca tcgccgaagt
     2041 cgaggcgcgc aaaggcgacg tcgtgtggga cgcgcctacg aactggacgc catgctggta
     2101 gctttcgggt gaacgcagcc gcccgctgcg atcatgaggg cggcgagcac ggcgtttttc
     2161 acttgctcag cagaagggcg gtgtgccttt gcccgagtgg ttgcacgacc tgcccttcgc
     2221 atggtgcgtc gtcgtcgcga tcgagcgccc cgacgcgctg cgcgcggcgg ctgccgcaac
     2281 cgctgcggcg cctatgctga tcgccggaac cttcaccggc gcgccgccgg ccgccgagcc
     2341 tacgcctgcg cccgacgccg ctccgccggc cgcgccggcc ccgcctgcgg tgccggctgc
     2401 gccgccggcg ccttgcgata aggcgacgct agaggaaagt aataccgaca ggatgaccgc
     2461 tagcttgctg aacgtctcca taaagacctc ctgaagttct tggcggggct ggcgcccctt
     2521 gtgtgcctgc tttgttaacc gacggtaccg tgaattattc cgcctgacaa gaccaagata
     2581 gtatttgcct tacggctcaa caagtttgcg gtaaagatgc cacgttgcat ggcccaatac
     2641 cggtaggacc acggcgaggc cgatcaaaaa caggatcgcc ccggccgcca gcagcaccac
     2701 aacaattagt ccccagagag ccatcattaa agtattggag ctgactgctt tgatcgaagc
     2761 gtgggccgcc gttatggcag tcaccggtct gtccagggcc aaaggaaagg agaccaccga
     2821 aatcgccagc gccgcgacgg cgaaaaagaa cccgagaaca gtaccgtaga agatcagcgc
     2881 accaccccgt cgggttgtga gtacctgggt cacaaagtct gagatcgaat ccggtgaaac
     2941 tgcgccgaac agaccaaagt agagggtttc cgccgcatac agccaagcga catagagcag
     3001 catcatcgtc accgaaagcg ccaggatggg agcgaatgag gaagagtgca caaaatcgaa
     3061 cgctgagcgc caggtgaggt cgagcccgcg ttcgcgacgt cggctcattt caaaaagacc
     3121 aaccgagacc acggggccga gaaaggtaaa gcccgcgacc atcggaaagg cgagatgcag
     3181 cagattgtcg ccaaccagaa gcagtgtcca gagcagggcg aacagcggat aaattacgat
     3241 caagaacgcg ataacacttg gtttggcatt aaagtcgtcg aagccttcct tcagcgcctg
     3301 ccagagatct ttcacgccga tcttgcggat tctgatctcg ctcgatctga cttgtctgtc
     3361 cctaaaagat ttttcctcag ccatgacgaa acccgctttt acctctttgg ttttttcacc
     3421 gatagcggct ctcgacgcga cataagcgcc attgtacgca gccgcgccac cgctgaggag
     3481 cgtaaggatg gccatctcac ggggcgccgt gcggccggcg gtggatagat cttcgtatcc
     3541 gttctagtgc gcctggtccc agttgtcgcc gacgccggcg tcgacgatca gcggaacatc
     3601 gagcttggcg accgattgca tcaattcacg gaccttttcc tttgcgacgg tcagttcgtc
     3661 ctccgggacc tcgaggacca gctcgtcgtg aacctgcatg attaacagcg tcttcagcct
     3721 cgattcgtcg agccatttct gcaccgagat catcgcaagc ttgatcaggt cggcggcggt
     3781 gccctgcatc ggcgcgttga tcgcggcgcg ctcggcggcc tggcggcgct gcgggctgcc
     3841 tgacttcatc tccggcaggt acagccgccg tccaaacacg gtctcgacgt aaccacgcgt
     3901 gcgcgcaagc tcgcgcgtgc ggtccatgaa gcgcttcacg ccggggtagc gcgagaagta
     3961 cgagtcgatg taggcctgcg cggtgacgcg ctcgatgccg aggttctgcg ccacgccgaa
     4021 gctcgacatg ccgtagatca ggccgaagtt gatcaccttg gcggcacggc gctcgtcatt
     4081 ggacacctgg tcgagcggca cgccgaatac ctcggcggcg gtggcgcggt gcacatcgtg
     4141 gccgcgcgcg aacgcctgct tgaggctctc gtctcccgag aggtgcgcca tgatgcgcag
     4201 ctcgatctgc gagtagtcgg cggagacgat cttcgcgccc gcgggcgcga tgaagcactc
     4261 gcggatgcgt cgcccggcgg cggtgcggat cgggatgttc tgcaagttgg ggtcgttcga
     4321 ggcgaggcgc ccggtgaccg cggtggtctg cgagtaggtg gtgtgcacgc gcccggtgcc
     4381 cggatggacg cttttcggca gcttgtcggt gtaggtcgac ttgagcttgg cgagcgcccg
     4441 gtgctcgaga atcagcctgg gcagcgggtg atccagcgcg agctcctcga gcacatcctc
     4501 gtcggtcgag ggctggccgg agggggtctt cttcttcacc ggcagctggt gtttgtcgaa
     4561 caggatctcc tggatctgct tcggcgagcc gaggttgaaa ggctgtcccg ccgcctcgtg
     4621 cgcctgccgc tccttcttca acacctcctt gccgagctcg tggctctgcg cctcgagctt
     4681 cgcggcatcg agcagcacgc cgttacgctc catgcggaac agcaccggca gcaccggcat
     4741 ctcgatcttc tcgtagacga agctgagctt ttcgtcctgc ttgatcttcg ggtagagcac
     4801 gtcgtgcacg gcgagcgtgc agtcggcgtc ctcggcggcg tattgcgtgg cgcgcccgat
     4861 ctccaccgcg gagaactcga tgcgcgcggc gcccttgccg gtaacgtcat cgtaactgag
     4921 cgtctgccag ccgcaatggc gctgcgcgag cacgccgagg tcgtggcgct cgtgcacttc
     4981 cagcacgtac gactcgagca gcgtgtcgtg cacgcacccg gcaagccgga ttccctggtt
     5041 ggccaggacg tgcgagtcga acttcacgtt ctgcccgacc ttgccgcgag cgtcgcgctc
     5101 gagccacggc ttcagggtct gcaacgcaag atccatgggc aactgcgcgg gagcgcccgg
     5161 atactcgtga gaaaggggca ggtaaaaagc ctggcgccca agggcgaatg acatgccgac
     5221 cagcctcgcc gtcatcggct ccaggccgac gcactcggta tcgaagccga ccagcggcgc
     5281 ggcttcgagc tgtttcacca gggctctcag ctcgtcctcg gtgagaatgg ccgagtattt
     5341 gcgtttttcc ggccccggtg tcctttcttc ggcaaccagc ggaatctcct gagaaggcgc
     5401 gggcgcggca ctttccgttt cgcgcagcca ggtcctgaag ccgaaccgct cgtagagcgt
     5461 gcgttgacgc gcggcgtcgg gcttgccttc ggcgagttgg tcgagggtta caggcagcgg
     5521 tacgtcgcgc ctgaccgtga cgagctccct cgcctttgga atccagtcga gtttctttcg
     5581 aagattttcg cctcgaacgc cgggaatctg gctcgcgttg gcgaccagat tgtccaaaga
     5641 cccatactgg ctcagccatt tcgaggcgaa accgggacca actccctcta cgcctggaat
     5701 gttatcgatt gcgtcgccta ccagggtcag gtagtcgacg atccggtctg gcggaacgcc
     5761 gaatttcttc ctgacgcctt cgatgtccag tatctcgttc gacatcgtat tgacccaggt
     5821 gacgtgctcg tcgacgagct gcgtcaggtc cttgtcgccg gtcgagatga tgctgcgcca
     5881 gccgcggcgc ttcgccagct cgacgagcgt ggcgatgacg tcgtcggcct cgacgccgtc
     5941 gatggtgagc accggccagc caagcgcctc gatcgcctcg cgcagcggct cgacctgcgc
     6001 gccaagatcg tcgggcatcg acgcgcggtt cgccttgtac gcgggatact gcgcgtcgcg
     6061 gaacgttttg cccttggcgt cgaaaacgca ggcgcgcgcc tgtgccttgt agtcggcgcc
     6121 aagcttccgc agcatgctga gcacgccgta gatggcaccg gtcggttcgc ctttcgggct
     6181 cttcagctcc ggcagcgcgt ggaacgcgcg gtacaggtag gacgagccgt caaccagcaa
     6241 caacgtattt tcaggatctt ttcccgacac gatgacacac gagaaactgc cgaaggcggc
     6301 cggcctcggc cacgagattt ccgcgcgcga ggcgtggaag ctgttcggca ttatggcaga
     6361 gttcttcgaa gtgacgttct agtagaatcg gcgcaggatg cgccggttac tcgtcttggt
     6421 gatgtttttt tcattgagcg tgatggcgca acagcggccg cccaagctcg agccgctgcc
     6481 cgagccgccg ccgccgccgc cgattcccgg acccgacgag ccggcggtac gcattccggt
     6541 acaggaagcc gacaaggtgg aggagatccg cgaaggcggg cgggtggtga tgctcaaggt
     6601 gaccccggcg ggcggcaaac cctattacct cgtagacacc accggcaacg gcaactggat
     6661 gcgccgcgat tcgctcgatg acggagtgcg agtgccgatg tggcccatcc acatcttcga
     6721 ctgactgccg gtccacctac cctgattgtc ggtttacacg cctgtatctg aagcgcagct
     6781 cgcggactgg ctgcgcaatt actcggtcgg cacgctggcg cgctgcgagc cgatcaaggc
     6841 cggcatcgag aacaccaatt acttcgtcac cacgacgcag gggcgctacg tcctgacgct
     6901 gttcgagcgc ctgcccgcgg acgagctgcc cttctacctc ggtctcatgg cgcatctcgc
     6961 gcggcacggc attccctgcc cggcgccgat cgcggacctg tcggatcaat acctgcagcg
     7021 gctgaatggc aagcccgccg cgctcgtcac ccgtctgccg gggcgctcgc tcgagaaccc
     7081 tggcgcgagc cattgcgccg aattgggcgc gctgcttgcg cgcatgcacc tcgcaggccg
     7141 ttcttacggc gcctacctgg aaaatccgcg cggcgcgaag tggtggcgct tcgccgcgcg
     7201 cgcggtcgcc ccgtttctcg atcctgaaaa tagatccctg ctggaaaatg aaattgcgtt
     7261 ccaggccgag caccgccttc ccgacctgcc acgtggcccg gtgcatgcgg atctgttccg
     7321 cgacaacgcg ctgttcgagg acgcccgcat ctcgggggtg atcgacttct acttag
//
LOCUS       DASPXW010000209         3468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1825716, whole genome shotgun sequence.
ACCESSION   DASPXW010000209 DASPXW010000000
VERSION     DASPXW010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1825716"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VEL04_04910"
     CDS             <1..336
                     /locus_tag="VEL04_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004567786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HYT47736.1"
                     /translation="LAKNLFLSGERSWPRKAQEALITWMLEGTMSKRRILELYLNVAE
                     WGEGVFGAEAAARYNFGVAAAALGPAQAAWLAATLPSPRRYARGRSTPYLEGRTATIL
                     ARMEGAQIP"
     gene            357..1727
                     /gene="mgtE"
                     /locus_tag="VEL04_04915"
     CDS             357..1727
                     /gene="mgtE"
                     /locus_tag="VEL04_04915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HYT47737.1"
                     /translation="MDEARARLETEELQDRLREVQELISDGKRPAELRARLDPLHPAD
                     VAYLLEALPLEERLAVWNLVKADRDGEILLEVSEAVRESLIASMDSAELVAAAETLEA
                     DELADIAADLPASVIEEVVQSLPAEERERLRAALSYPEGSVGALMDFDHVAVREDVTL
                     EAATRYLRRLDELPDHTDQLFVVDRGQALKGTLPLSRLIVSDLELEVTRVMVPESVKL
                     HPEDPAEDAANAFERYDLVSAPVVDGGGRLIGRLTVDAVVDYIREKSAESQLAEAGLR
                     EAEDVFAPVLDSFKNRWAWLAINLVTAFIASRVIGVFEHSIAQLVALAALMPIVAGIG
                     GNSGNQTITMIVRAMALGRIQAPYWGKLLAKELGVALLNGVVWGSLLGAIAYAFYGKV
                     ALGGVMALAMTLNLMLAALMGVAIPWLRTRFGRDPAVGSSVLITACTDSGGFFIFLGL
                     ATVFLV"
     gene            complement(1894..3177)
                     /gene="hemL"
                     /locus_tag="VEL04_04920"
     CDS             complement(1894..3177)
                     /gene="hemL"
                     /locus_tag="VEL04_04920"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HYT47738.1"
                     /translation="MKQSSASLFERALRTIPGGVNSPVRAFGAVGGTPVFFERAAGAY
                     LWDAEGKRYIDYLGSWGPMVAGHSHPAVVEAVQEAASRALSFGAPTAAEVELAETLCR
                     LVPSLETVRLLSSGTEATMTAIRLARGFTGRNLIVKFEGCYHGHADALLVKAGSGALT
                     FGNPSSAGVPAEAAAHTLVLDYNDASRVKKLFAEKGRGIGCVIVEPVAGNMNLVLPAA
                     GFLETLREECTRHGALLIFDEVMTGFRVARGGAQARFGIQPDLTTLGKVIGGGLPVGA
                     LGGRREIMQKLAPLGPVYQAGTLSGNPVAVAAGLATLKIVQAEEFQENVEGETQALVE
                     GLIAQAKKTSVTFSSQSMGSMFGIYFRNAPPRSFAEVMQCDRERFKRFFHAMLERGVY
                     LAPSAYEAGFVSAAHGDAEIEATLAAARGAFAQIA"
     gene            complement(3174..>3468)
                     /locus_tag="VEL04_04925"
     CDS             complement(3174..>3468)
                     /locus_tag="VEL04_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HYT47739.1"
                     /translation="CYDRLELAHTAVAAGADYVAFGSVFASPTKPHAVRAALSLFGQA
                     RTLGVPLAAIGGITLGNARQAIDAGADLLAVISDLFDAEDIAAHAREYQKLFR"
BASE COUNT          564 a         1142 c         1187 g          575 t
ORIGIN      
        1 ctggcgaaga acctgttcct ctccggggag cgctcgtggc cgagaaaggc gcaggaagcg
       61 ctgatcacct ggatgctcga gggcaccatg tccaagcgcc gcatcctcga gctgtatctc
      121 aatgttgcgg agtggggcga aggcgtattc ggcgccgagg cggcggcgcg ctacaacttc
      181 ggggttgcgg ccgcggctct cggtccggcg caggcggcgt ggctcgccgc gaccctgccg
      241 agcccgcgac gctatgcgcg cggccgcagc acgccctacc tcgaagggcg gaccgcgacc
      301 atccttgctc gcatggaagg cgcgcagatt ccctgaatct ggcaggcttc ggccccatgg
      361 acgaagcgcg cgcgcgcctg gaaaccgagg agctgcagga tcggctgcgc gaggtccagg
      421 agctgatttc ggacggcaag cgtcccgccg agctgcgcgc caggcttgac ccgctgcatc
      481 ccgccgacgt cgcctatttg ctcgaggcgc tgccgctcga ggagcggctc gccgtatgga
      541 acctggtcaa ggccgaccgc gacggcgaaa ttctgctcga agtgtccgaa gccgtgcgcg
      601 agtcgctcat tgcgagcatg gactcggccg agctggtggc ggcggccgag accctcgaag
      661 ccgatgagct cgccgacatt gctgccgacc ttccggcgag cgtcatcgag gaagtggtgc
      721 agtcgctgcc cgccgaggag cgcgagcggc tgcgcgcggc cctgtcttat cccgagggct
      781 cggtcggcgc gctgatggac ttcgaccatg tcgcggtgcg cgaggacgtg acgctcgagg
      841 cggcgacgcg ctatctgcgc cgcctggacg agctgcccga ccataccgac cagctgttcg
      901 tcgtcgaccg cggtcaggcg ctcaaaggca cgctgccgct ctcacgcctc atcgtctccg
      961 acctcgagct cgaagtgacg cgagtcatgg tgccggaaag cgtcaagctg cacccagagg
     1021 acccggccga ggacgccgcc aacgcgttcg agcgttacga cctggtctcg gccccggtgg
     1081 tcgacggggg cgggcgcctg atcggcaggc tcaccgtcga cgcggtggtc gactacattc
     1141 gcgagaagag cgccgagtcg cagctcgccg aggcgggctt gcgcgaggca gaagacgtgt
     1201 tcgcaccggt gctcgacagc ttcaagaacc gctgggcgtg gctggcgatc aacctggtca
     1261 ccgccttcat cgcctcgcgg gtcatcggtg tcttcgagca ttcgattgcg cagctcgtcg
     1321 cgctcgcggc gctgatgcca atcgtcgccg ggatcggcgg caactcgggc aaccagacga
     1381 tcacgatgat cgtgcgcgcc atggctctgg gtcggatcca ggcgccgtac tgggggaagc
     1441 tgcttgccaa ggagctcggc gtggcgctgc tgaacggcgt ggtgtgggga agcctgctcg
     1501 gcgcgatcgc gtacgcgttc tacggcaaag tcgcgctggg cggcgtcatg gcgctcgcga
     1561 tgacgctcaa cctgatgctc gccgcgctga tgggcgtcgc gatcccgtgg ctgcgcacgc
     1621 gcttcgggcg cgacccggcg gtaggctcct cggtgctgat cacagcgtgc accgactcgg
     1681 gcggcttctt catttttctt ggtctggcga cggtgtttct ggtgtgagcg taccggcgac
     1741 acgatgcgcg cgacataggc gcgcggcgct gcatcgttga gcgaggcgcc gaagcgcagc
     1801 ctgcggtctt cggattcgag cgagagaaaa tggcgctcga gcgccggccg gtcgagcctg
     1861 gaaagttccc tgatgtggat gtccatcttt tcgctaagcg atctgtgcga atgcgccgcg
     1921 cgccgcggcg agcgtagctt cgatctcggc gtcaccgtga gcggccgaca cgaatcccgc
     1981 ttcgtaggcc gagggcgcaa ggtacacgcc gcgctcgagc atcgcgtgga agaagcgttt
     2041 gaaacgctcc cgatcgcatt gcatgacttc ggcaaaactc cgcggcggcg cgttgcggaa
     2101 atagatgccg aacatgctgc ccatcgattg cgacgagaac gtgacactgg ttttttttgc
     2161 ctgcgcaatg aggccttcca ccagagcctg ggtttcacct tctacatttt cctgaaactc
     2221 ttcggcttgc acgattttga gagtcgccag gcccgcggca acggcaactg gatttcccga
     2281 aagcgtgccc gcctggtaaa ccggcccgag cggcgcgagc ttctgcatga tctcgcggcg
     2341 gccgccgagc gcgcccaccg gcagcccacc gccgatcacc ttgccgaggg tggtgagatc
     2401 gggctggatg ccgaagcgcg cctgtgcacc gccgcgcgcg acgcgaaagc cggtcatcac
     2461 ctcgtcgaag atcagcagcg caccgtggcg cgtgcattct tcgcgcagcg tctccaggaa
     2521 gccggcggcg ggcaggacca ggttcatgtt tcctgccacc ggttcgacga tgacgcagcc
     2581 gatcccgcgg cctttctccg cgaacagctt tttgactcgc gaagcatcgt tgtaatccag
     2641 aaccaaggta tgtgcggccg cctcggcggg cacgccggcc gagctcgggt tgccgaaggt
     2701 gagggcgccc gatcctgcct tgacgagcag cgcgtcggcg tggccgtgat agcagccttc
     2761 gaacttgacg atcaggtttc tgcccgtaaa gcccctcgcg aggcggatcg cagtcatggt
     2821 cgcctcggtg cccgaggaca agagccgcac cgtctcgagc gagggcacca ggcggcagag
     2881 cgtctccgca agctcgacct cggctgcggt gggcgcgccg aaggagagcg cgcgcgaggc
     2941 ggcctcctgc accgcttcga ccaccgccgg atggctatgg ccggccacca tcggtcccca
     3001 ggagccgagg tagtcgatat accgcttgcc ttccgcgtcc cagaggtagg cgcccgcggc
     3061 acgctcgaag aacaccggcg tgccgccgac cgctccgaag gcccgcaccg gcgaattcac
     3121 gccacccgga atcgtccgca gcgcacgctc gaaaagggaa gcgctgcttt gcttcatcgg
     3181 aacagcttct ggtattcgcg cgcgtgggcg gcgatgtcct cggcgtcgaa caaatcggaa
     3241 atcaccgcga gcagatcggc accagcgtcg atcgcctggc gcgcgttgcc gagcgtgatg
     3301 ccgccaatcg cggcgagcgg aacccccagc gtgcgtgctt ggccgaacag cgaaagcgcg
     3361 gcccgcacgg cgtgaggctt ggtgggcgag gcgaacacgc tgccgaacgc gacgtaatct
     3421 gctccagctg ctaccgccgt gtgcgcgagc tcgagcctgt cgtagcac
//
LOCUS       DASPXW010000210         5105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_313757, whole genome shotgun sequence.
ACCESSION   DASPXW010000210 DASPXW010000000
VERSION     DASPXW010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5105
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313757"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VEL04_04930"
     CDS             complement(<1..534)
                     /locus_tag="VEL04_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47740.1"
                     /translation="MRRLAALWTKEWLALARDLHGLAVLFLMPAVFIVVMSLALSDVF
                     RDNAGRGVDFAVLAADGAQAAEQLAQSIAGDGFRATPAPPSEAAARDGVRKGRPGLVL
                     VVPAGFGADPAAPLKLLADPALPPMQLAAFQHRVLAATLGLKASALAQALNIEVVGNP
                     RGGRPSSVQQNVPAWLIF"
     gene            complement(534..1373)
                     /locus_tag="VEL04_04935"
     CDS             complement(534..1373)
                     /locus_tag="VEL04_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT47741.1"
                     /translation="MLKVEALSFRYPGRGEAVLDDVTLAIPAGGIFGLLGPNGAGKTT
                     LISILAGQLRGAAGRVTVGGEALDDLRARRPHCLGLVPQEFAFYPMLTARENLRFFGG
                     VQGLGRARLTQRVAAVAAFARIENVLERRAAELSGGLKRRLNLAIGLLTEPEILLLDE
                     PTVGVDPQSRAFLLESIRSLARAGRIIVYTSHYMEEVQAICDRVAIIDAGRVLASAPL
                     AELLREDSGVVTLRLRAPGPEAIARAIEAARAAGCEPLAVDRGSRDLEQVFMQLTNRS
                     LRD"
     gene            complement(1367..2374)
                     /locus_tag="VEL04_04940"
     CDS             complement(1367..2374)
                     /locus_tag="VEL04_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HYT47742.1"
                     /translation="MARAFVHAHASHCESGVMAALVRYHGLPMSEAMAFGLGSALCFA
                     YLPFIRINGLPLIAYRMPPRAIINSLHRSLGLGMRFEKFRSPERGMARLDALLDAGEL
                     VGLQTSVFWLPYVPPDMRFHFNAHNLLVCGREGDDYLISDPLLETVVRCGRKDLEKAR
                     FAKGLLAPKGLLYYATAKPVSPDWARLGRRALARTARIMLHTPLPLIGVRGVRLLARS
                     VERLSLADGAESARLYVGNIVRMQEEIGTGGAGFRFLYASFLQELAAATGYGALDRLA
                     SRLVEIGDRWREFALAAARMIRGRDAFAPPALAAHLRALAAEEKQFFRDVDAAQRAWA
                     C"
     gene            complement(2376..2771)
                     /locus_tag="VEL04_04945"
     CDS             complement(2376..2771)
                     /locus_tag="VEL04_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47743.1"
                     /translation="MRERDVPQEGNASLGGLRRAVYAVAEDGKLRLVLSRGWEVEEIV
                     ARQAVEDLERRAQAARARVLSGETSPLEYHMYRARMDLALLSQATGLWRWRVRRHFRP
                     QVFRRLPPALRRRYAEALGILPEALDKVE"
     gene            complement(2764..3915)
                     /locus_tag="VEL04_04950"
     CDS             complement(2764..3915)
                     /locus_tag="VEL04_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HYT47744.1"
                     /translation="MRPAAYITRTSVCLPNAPVENGAMEALLGQVGGRASRARRVVLR
                     NNGIQRRYYVIDPATGEPNYSNASLTAAAIRQLAGDGFALEDIACLACGTSNPDQLMP
                     NHAVMVHGELGTPVCEVVATAGVCLSGTTALKYGWMAVQSGVARNAVVTGSEVASLGL
                     RALNYDAECPGDAELEAHPELAFDKDFLRWMLSDGAGAWLLEPAPRCGLNLRIEWIDI
                     FSYAHALPACMYMGAEKQADGALRSWMRYSARERESISVFALKQDVRLLNEAVVEYTL
                     VKPLQRLLAQRRLAARDVDWFLPHMSSEYFRARVDEGLARAGLPIPQERWFSNLASTG
                     NTGSAAVYIMLDELLRSGKLRDGQHLLCFVPESGRFSSGFIYLTASAHA"
     gene            complement(3917..4834)
                     /locus_tag="VEL04_04955"
     CDS             complement(3917..4834)
                     /locus_tag="VEL04_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dialkylresorcinol condensing enzyme"
                     /protein_id="HYT47745.1"
                     /translation="MKRVLVVHFSQTGQLARVARRLSSPLAEAPDVELVEEVLRPSRP
                     YPFPWPAWRFLDAMPESVLLEPPALEPLRVRADEHFDLIVLAYQVWFLAPSGPITAFL
                     KSEAGRQLLRGRPVVTLIACRNMWLTAQETVKRLLREAGGRLRDNVVFSDRGSAMASL
                     ITTPRWLLTGRREAFWGLPAAGVAEEDIAGADRFGRALLAALRDGRERAAEPMLAGLG
                     AARVDPRLIFSERAGCRAFALWSRLIRLGGPQGSAGRVPLLALFCVYLVAMILAVVPA
                     SLLLQRLLRPLLAARLELEKTYFELPSGR"
     gene            4923..>5105
                     /locus_tag="VEL04_04960"
     CDS             4923..>5105
                     /locus_tag="VEL04_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYT47746.1"
                     /translation="MRKLIVALALSAFAGFTGAQAQSFPSRPITLVSPFPPGGSTDTA
                     ARIIAERMRAPLGQSVV"
BASE COUNT          829 a         1800 c         1805 g          671 t
ORIGIN      
        1 gaagatcagc caggccggca cgttctgctg caccgacgaa ggccgcccgc cgcgcggatt
       61 gccgaccacc tcgatgttca gcgcctgcgc cagcgccgag gctttcaggc cgagggttgc
      121 ggcgaggacc cgatgctgga aggcggcgag ctgcatcggc ggcagcgccg gatcggcgag
      181 cagcttcagc ggggcggccg ggtcggcgcc gaacccggcc ggcacgacga gcaccagccc
      241 cggccggccc ttgcgcacgc cgtcgcgggc ggcggcctcg ctggggggag cgggcgtggc
      301 gcggaaaccg tcgccggcaa tgctctgcgc gagctgctcc gcggcctgcg cgccgtcggc
      361 ggcgagcacc gcaaaatcga ctccccggcc ggcgttgtcc ctgaagacat cgctcagggc
      421 gagcgacatc acgacgatga acacggccgg catcaggaac agcaccgcga gcccgtgcag
      481 gtcgcgcgcc agggcgagcc attccttggt ccacaacgca gccaggcgcc gcatcaatcg
      541 cgcagcgagc ggttggtgag ctgcatgaag acctgctcga ggtcgcggct gccgcgatcg
      601 acggcgagcg gctcgcagcc cgcggcgcgc gccgcctcga tcgcccgcgc gatcgcctcc
      661 ggcccgggcg cgcgcagccg cagggtgacg acgcccgaat cctcgcgcag cagctcggcg
      721 agaggcgcgc tcgccaatac gcggccggcg tcgatgatcg ccacgcgatc gcagatcgcc
      781 tgcacctcct ccatgtagtg gctggtgtag acgatgatcc ggcccgctcg cgcgagcgag
      841 cggatcgact ccagcaggaa tgcgcgcgac tgcgggtcga cgcccaccgt gggctcgtcg
      901 agcagcagga tttcgggttc ggtgagcagg ccgatggcga gattcaggcg gcgcttgagg
      961 ccgccggaaa gctccgcggc gcgccgctcg agcacgttct cgatgcgggc gaacgcggcc
     1021 acggccgcga cgcgctgcgt aaggcgtgcg cggccgagcc cttgcacgcc gccgaagaag
     1081 cgcaggttct cgcgcgcggt gagcatcggg tagaaggcga attcctgcgg caccaggccg
     1141 aggcaatgcg gccggcgcgc gcgcaggtcg tccagcgcct cgccgccgac cgtgacgcgt
     1201 cccgcggcgc cgcgcagctg cccggcgagg atcgagatca aggtggtctt gccggcgccg
     1261 ttcggcccga gcaagccgaa gatgccgccg gcggggatgg cgagcgtgac gtcgtccagc
     1321 accgcttcgc cgcggcccgg atagcggaac gacagcgcct cgaccttcag catgcccagg
     1381 cgcgctgcgc ggcgtccacg tcgcggaaga actgcttttc ctcggccgcg agcgcgcgca
     1441 ggtgcgccgc gagcgccggc ggagcgaagg catcgcggcc gcggatcatg cgcgccgcgg
     1501 cgagcgcgaa ctcgcgccag cggtcgccga tctcgaccag gcgagaggcg agccgatcga
     1561 gcgcgccgta gccggtcgcg gcagcgagct cctgcaggaa cgacgcgtac aggaagcgga
     1621 agcctgcgcc gccggtgccg atctcctcct gcatgcgcac gatgttcccg acgtaaagcc
     1681 gcgccgactc ggcgccgtcg gcgagcgaga gccgctcgac gctgcgcgcg agcagccgca
     1741 cgccgcgcac gccgatgagc ggcagcggcg tatgcaacat gatgcgcgcg gtgcgcgcga
     1801 gggcgcgccg cccgagacgc gcccagtccg ggctcacagg cttcgcggtc gcgtagtaaa
     1861 gcaggccttt gggcgccagc agccccttgg cgaagcgcgc cttctccagg tccttgcggc
     1921 cgcagcgcac caccgtctcc aacaacgggt cgctgatcag gtagtcgtcg ccttcgcggc
     1981 cacatacgag caggttgtgc gcattgaagt gaaagcgcat gtcgggcggg acatacggca
     2041 gccagaacac cgaggtctgc agcccgacca gctcgccggc gtcgagcagc gcgtcgagcc
     2101 ttgccatgcc gcgctcgggc gagcggaact tctcgaagcg catgccgagc cccaggctgc
     2161 ggtgcaggct attgatgatg gcgcgcggcg gcatgcggta ggcgatcagc ggcagcccgt
     2221 tgatgcggat gaaaggcagg taggcgaagc acagcgccga cccgaggccg aaggccatcg
     2281 cctcggacat cggcaggccg tgataacgca ccagcgcggc catcacgccc gactcgcaat
     2341 gcgaggcgtg cgcgtgcacg aacgctcgcg ccacgctact ccaccttgtc cagcgcctcg
     2401 gggaggatgc cgagcgcttc ggcgtagcgc cgcctcagcg cggggggcag gcgcctgaac
     2461 acctgcggcc ggaagtggcg ccgcacgcgc cagcgccaca ggccggtggc ctgcgacagc
     2521 agcgcgaggt ccatgcgcgc ccgatacatg tggtattcga gcggcgaagt ctcgcccgag
     2581 agcacgcgcg cgcgcgcggc ttgcgcgcgc cgctccaggt cctcgaccgc ctgccgcgcc
     2641 acgatctcct cgacttccca gccgcgcgag agcaccagcc gcagcttgcc gtcctcggct
     2701 actgcataga ccgcccggcg caggccgccg aggctggcgt tgccctcctg cggcacgtcg
     2761 cgctcacgca tgcgcgctcg ccgtgaggta gatgaagccg ctcgagaagc ggccgctttc
     2821 cggcacgaag cacagcaggt gctggccgtc gcgcagcttg ccgctgcgca ggagctcatc
     2881 gagcatgatg taaacggcgg ccgagccggt attgccggtc gaggcaaggt tgctgaacca
     2941 gcgctcctgc gggatcggca ggccggcgcg cgccagccct tcgtccacgc gcgcgcggaa
     3001 atattccgag gacatgtggg gcaggaacca gtcgacgtcg cgcgccgcga ggcgccgctg
     3061 ggcaagcagc cgctgcagcg gtttcaccag cgtgtactcg accaccgcct cgttgagcag
     3121 ccgcacgtcc tgcttgagcg cgaacaccga gatcgattcg cgctcgcgcg ccgaatagcg
     3181 catccagctg cgcagcgcgc cgtcggcctg cttttccgcg cccatgtaca tgcatgccgg
     3241 cagcgcgtgc gcataggaaa agatgtcgat ccattcgatg cgcaaattga gcccgcagcg
     3301 cggcgcaggc tcgagcagcc aggcgccggc gccgtcggac agcatccagc gcaggaaatc
     3361 cttgtcgaaa gcgagctcgg gatgggcctc gagctcggcg tcgcccgggc attcggcgtc
     3421 gtagttcagc gcgcgcaggc cgagcgaggc gacctcggag ccggtgacga ccgcgttgcg
     3481 cgcgacgccg gactggaccg ccatccagcc gtatttcagc gcggtggtgc ccgacaggca
     3541 gactcccgcg gtcgcgacca cctcgcacac cggcgtgccg agctcaccgt gcaccatcac
     3601 ggcatggttc ggcatcagct gatcggggtt cgaggtgccg caggcgaggc aggcgatgtc
     3661 ttcgagcgcg aagccgtcgc cggccagttg cctgatcgcg gcggcagtca ggctggcatt
     3721 ggaatagttc ggctcgccgg tcgccggatc gatcacgtaa tagcggcgct ggatgccgtt
     3781 attgcgcagc acgacgcgcc gtgcgcgcga cgctcgcccg ccgacctggc ccagtagcgc
     3841 ctccatcgcg ccgttctcca ccggagcgtt cggcaggcag acggaggtgc gcgtgatgta
     3901 ggcggccggt ctcacgctag cgtccggacg gaagctcgaa ataggtcttt tccagttcca
     3961 gtcgagccgc cagcagcggg cgcagcagcc gctgcaacag caggctcgcc ggcaccacgg
     4021 cgagaatcat cgccaccaga tagacgcaaa aaagcgcgag cagcggcacg cggccggccg
     4081 agccttgcgg cccgcccagg cggatcaggc gcgaccacag tgcgaacgcc cggcagccgg
     4141 cgcgctcgct gaagatcagc cgcgggtcga cgcgcgccgc gcccaggccg gcgagcatcg
     4201 gctcggccgc gcgctcgcgg ccgtcgcgca acgcggcgag cagcgcccgg ccgaagcgat
     4261 cggcgcccgc gatgtcttcc tcggccacgc cggcggcggg cagaccccag aacgcctcgc
     4321 gccggccggt cagcagccag cgcggcgtgg tgatcaatga cgccattgcg cttccccggt
     4381 cgctgaacac gacattgtcg cgcagccgac cgcccgcttc gcggagcagg cgcttcaccg
     4441 tttcctgcgc cgtcagccac atgttgcggc atgcgatcag cgtcaccacc ggccggccgc
     4501 gcagcagctg cctgccggcc tcgctcttga ggaacgcggt gatcggtccc gacggcgcga
     4561 ggaaccagac ctgataggcg agcacgatga gatcgaagtg ctcgtcagcg cgcaccctga
     4621 ggggctcgag cgccggcggc tcgagcagca ccgactccgg catcgcatcg aggaagcgcc
     4681 acgccggcca gggaaacggg tagggccggc tcggccgcag cacttcctcg accagctcga
     4741 cgtcgggcgc ttcggcgagc ggcgacgaca gccgccgcgc gacgcgagcg agctgcccgg
     4801 tttgcgagaa atgcacgacc agaacgcgtt tcattttaag ctgagagaat aactcggcgc
     4861 ggggacggat gtcattggat tttctggcat tgttgtaaaa actgcattac cttgagagga
     4921 gcatgagaaa actaatcgtt gcgctcgcgc tatcggcgtt tgccggattc accggggcgc
     4981 aggcgcaaag ctttccatcg cggcccatta ccctcgtctc gccgttcccg ccgggcggct
     5041 cgacggatac ggcggcgcgc atcatcgccg agcgtatgcg cgcgccgctc ggccagagcg
     5101 tcgtg
//
LOCUS       DASPXW010000211         2522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_693009, whole genome shotgun sequence.
ACCESSION   DASPXW010000211 DASPXW010000000
VERSION     DASPXW010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693009"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..877)
                     /locus_tag="VEL04_04965"
     CDS             complement(44..877)
                     /locus_tag="VEL04_04965"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HYT47747.1"
                     /translation="MNGSTLASTAELSQNLGKWRVFDCRHDLAKPGLGEQQYRAAHIP
                     GAAFAHLDRDLSAPKTGRNGRHPLPDPAVFVSWLGKQGLRKDGRVVCYDGGNGAMAAR
                     LWWMLRWVGHEAVAVLDGGLAKWLSEGRPVTADIPSFEPSNFPLKLNAGAALSADAVQ
                     KNLKKLLLIDARAPARYRGEQEPIDPVAGRIPGALNRFNADNVSADGTFKPASELKRE
                     FQTLLAEAKNEEIVHYCGSGVAACHNLLAMEIAGLPGARLYAGSWSEWSADPKRPQER
                     G"
     gene            complement(840..1943)
                     /locus_tag="VEL04_04970"
     CDS             complement(840..1943)
                     /locus_tag="VEL04_04970"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003275734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HYT47748.1"
                     /translation="MTDVASLRQLRRANCHLPVSWYFDAAVFAREKKLLFDAGANYVG
                     HELMVPNVGDYHALAWAGDAKVLVRNPSGVELLSNVCRHRQAILLEGRGHVTHIVCPL
                     HRWTYDLKGELLGAPHFPDSPCVKLHSTPLTKWNGLLFAGPRDPRQDLARITTSADWD
                     FTGYVLDSVRIDEYDVNWKTFIETYLEVYHVDFYHPGLGNFTDCNNFTVDYGAEHSVQ
                     IVAAKAGLHRPGTPVYKKWHDACLRQLDGREPKHGALWATYFPGLTFEWYPNVLVVSR
                     LIPRSPARTTNVVEFYYPEEIALFEREFVEAQQAAYAETAIEDHEICMRLDRGRRALY
                     EQGLDDAGPYQSPMEDAEVHFHEWLHARLDGGT"
     gene            1984..2223
                     /locus_tag="VEL04_04975"
     CDS             1984..2223
                     /locus_tag="VEL04_04975"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007733039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HYT47749.1"
                     /translation="MNKPSGELTFEKALAELEKIVSRMEGGELSLEQALATHKRGLEL
                     ARFCQQRLEAAQQQVKVLEGEVLKPLSEAGETDRD"
     gene            2237..>2522
                     /locus_tag="VEL04_04980"
     CDS             2237..>2522
                     /locus_tag="VEL04_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HYT47750.1"
                     /translation="MGGVQARMESALARLLPAAHVAPARLHEAMRYAALEGGKRVRPL
                     LAFAAGELAGAPRERLEIVGAAVELIHAYSLVHDDLPCMDDDALRRGKPTV"
BASE COUNT          426 a          794 c          859 g          443 t
ORIGIN      
        1 cgcggctata cggcttctag ccgcgttcct gcggtctctg cattcagccg cgttcttgcg
       61 gcctctttgg atcggcgctc cactcgctcc acgacccggc gtagagcctg gcgccaggga
      121 gccctgcgat ctccatcgcg agcaggttgt ggcaggcggc aacgccggag ccacagtagt
      181 ggacgatttc ctcgtttttc gcttctgcaa gcaatgtctg aaattcgcgc ttcaattccg
      241 aggcaggctt gaaggtaccg tccgctgaaa cattgtcggc gttgaagcga ttgagcgcgc
      301 cgggaatgcg gccggcgacc gggtcgatcg gctcctgctc gccgcggtag cgggctggag
      361 cgcgggcatc gatgagcagc agttttttca gatttttctg gaccgcatcc gctgacagcg
      421 cggcaccagc gttgagcttc aatggaaaat tcgaaggttc gaacgacggt atgtcggcag
      481 tcaccggccg gccttcgctg agccacttgg cgagcccgcc gtcgagcacg gcgaccgctt
      541 cgtgtcccac ccagcgcagc atccaccaca ggcgcgccgc catcgcgccg ttgccgccgt
      601 cgtagcagac gactcgacca tcttttctca ggccctgctt gccgagccag gagacaaaaa
      661 ctgcggggtc gggaagcgga tgccggccgt tcctgccggt cttgggcgcc gagaggtcgc
      721 ggtcgaggtg cgcaaaagcg gcgcccggga tgtgcgctgc gcggtactgc tgctcgccga
      781 ggccgggctt ggcgaggtcg tggcggcagt cgaacacccg ccacttgccg aggttctgac
      841 taagttccgc cgtcgaggcg agcgtggagc cattcatgga agtgcacctc ggcgtcctcc
      901 atcggcgact ggtaggggcc ggcgtcgtcg agcccctgct cgtagagcgc gcggcggccg
      961 cggtccaggc gcatgcagat ctcgtggtcc tcgatcgcgg tctcggcgta ggccgcctgc
     1021 tgcgcctcga cgaactcgcg ctcgaacagc gcgatctcct cgggatagta gaactcgacc
     1081 acgttggtgg tgcgcgccgg cgagcgcggg atcagccgcg acacgacgag gacgttcggg
     1141 taccactcga aagtcaggcc cggaaaatac gtcgcccaaa gggcgccgtg cttcggctcg
     1201 cggccgtcga gctgcctgag gcaggcgtcg tgccatttct tgtagaccgg tgttcccggc
     1261 cgatgcaacc ccgccttagc cgccacgatc tggaccgaat gctccgcgcc gtagtcgacc
     1321 gtgaagttgt tgcagtcggt aaagttgccg agccccggat gatagaagtc gacgtggtag
     1381 acctcgagat aggtttcgat gaaggtcttc cagttgacgt cgtattcgtc gatgcgcacc
     1441 gagtcgagca catagcccgt aaagtcccag tcggcgctcg tcgtgatgcg tgcgaggtcc
     1501 tggcgcggat cgcgcggccc ggcgaagagc agcccgttcc atttggtcag cggcgtggag
     1561 tgcagcttca cgcacgggct gtcgggaaag tgcggcgcgc cgagcagctc gcctttcagg
     1621 tcgtaggtcc agcggtgcag cggacaaaca atatgggtca catggccgcg gccctcgagc
     1681 aggatcgcct ggcggtggcg gcagacattg gacagcagct cgacgcccga agggttcctg
     1741 accagcacct tcgcgtcgcc ggcccaggcg agcgcgtggt agtcgccgac gttcggcacc
     1801 atcagctcgt ggccgacgta gttggcgccg gcgtcgaaca gcagcttctt ttcccgcgcg
     1861 aaaaccgccg catcgaagta ccaggacacc ggcaggtggc agttcgcgcg cctgagctgg
     1921 cgcaggctgg ctacgtcagt catgcgttgt aaagccccta gaaaaacagt attttactaa
     1981 aagatgaata agccttcagg cgagctgact ttcgagaagg cgctcgcgga gctggaaaaa
     2041 atcgtctcgc gcatggaagg cggcgagttg tcgctcgagc aggcgctcgc cacccacaag
     2101 cgcgggctcg agctcgcgcg tttctgccag cagcgtctcg aggcggcgca gcagcaagtc
     2161 aaggtgctgg aaggcgaagt tctcaaaccg ctgtccgaag ccggtgaaac tgaccgagac
     2221 tgacttccag gcctggatgg ggggcgttca ggcgcgcatg gaaagcgcgc tcgcgcgcct
     2281 gctgccggcg gcgcatgtcg cgcccgcgcg cctgcacgag gcgatgcgct acgccgcgct
     2341 cgagggcggc aagcgcgtgc gtccgctgct cgcgttcgcg gcgggggagc tcgccggcgc
     2401 gccgcgcgag cggctcgaga tcgtgggcgc ggccgtagag ctgatccacg cgtattcgct
     2461 ggtgcatgac gacctgccct gcatggacga cgacgcattg aggcgcggca agccgaccgt
     2521 gc
//
LOCUS       DASPXW010000212         3630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1826611, whole genome shotgun sequence.
ACCESSION   DASPXW010000212 DASPXW010000000
VERSION     DASPXW010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1826611"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..863
                     /locus_tag="VEL04_04985"
     CDS             54..863
                     /locus_tag="VEL04_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HYT47751.1"
                     /translation="MNRSIERTLHRAAATIAATIVATAATAAAAAGVSCHAQSGPRTA
                     ALLELYTSEGCDSCPPADRWLSGLGARGYAPGRVVPLALHVDYWDYIGWKDPYARREF
                     SLRQRKLSQLQRLALVYTPQVLLQGRDFRDWGGAAFETAVSRINAQPARARIALEIHS
                     LEVSRLDVAVSAELLDLSHQGDAVVYLAAYENRLVSRVTAGENRGRTLGHDYVVLEWQ
                     GPFALGPRLAERRALPLLPAGVPANSGVVGFVQNRRTAEVLQALMLPACKG"
     gene            953..2080
                     /locus_tag="VEL04_04990"
     CDS             953..2080
                     /locus_tag="VEL04_04990"
                     /inference="COORDINATES: protein motif:HMM:TIGR03187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein"
                     /protein_id="HYT47752.1"
                     /translation="MQATQIRQKDGVFYFVSYRAKDLLAKVRFISRFYGEGEEIAPGR
                     IAQDDDIAQFIARIERNDHAFQRSLSRSKVRQLKNFYETAITQPPIPGTVLLFTSERL
                     SFRAGGEGGGAGTLNEPSSKYLIIDGQHRLAALQFYLHERPDDAANISVPCILFDGRS
                     EDFATEMFVIINSTPTRINKSHLVDLYEHVSFAAPDRKFAARLVERLYSEGDSPLRYR
                     INRLGGRSQRDKWVLQAELFNELHRWVRGRWRSIQLAGGSSKEVPRYYAIVRDFFKAA
                     RAVFGDAVWGKDNYMVTKPVTIKAMVRVCADLARDDAEPEAGRVARWEQRLGPWAEMA
                     RQFREEGFYERFPAKGEVERVARVHRDLARAAGIESSRPKP"
     gene            2124..2384
                     /locus_tag="VEL04_04995"
     CDS             2124..2384
                     /locus_tag="VEL04_04995"
                     /inference="COORDINATES: protein motif:HMM:NF022426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HYT47753.1"
                     /translation="MKTPLLLAFASLTMAGCAGMDAGECRSANWYDLGFRDAIFGMQR
                     MDEVYASQCGTHGAEPDRVRYAQGWHEGVYEFDQRKAHGGHD"
     gene            2368..2643
                     /locus_tag="VEL04_05000"
     CDS             2368..2643
                     /locus_tag="VEL04_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47754.1"
                     /translation="MGDTTKALVTAACLLVGGCTAMSESECRGADWYQLGYRDADPYG
                     LRPQIDLYAHQCRAWVQASESEYMRGWIDGYREWNTRVHGSECCGAR"
     gene            complement(2640..>3630)
                     /locus_tag="VEL04_05005"
     CDS             complement(2640..>3630)
                     /locus_tag="VEL04_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HYT47755.1"
                     /translation="SKESLALDVKHPAAKQILEKLLSKADIFLQNLAPGAAERLGLGA
                     AELRARHPRLIWCGISGYGPAGPYAEKKAYDLLVQCEAGMLSITGTPEAPSKAGIPVA
                     DIAAGMYAFSAILAALYRRSRDGQGATLDITMFEALGEWMGFPAYYTAYGGTPPPRSG
                     AHHATIAPYGPFRVGDGGTVFLSVQNEREFGRFCDIVLQDRSLAADPRFSSGPARLQN
                     RAALHAHIDQAFANMKTQEVIERLETADVANARLNSMHEFWRHPQLEARRRWAKVGSP
                     AGELDALKPPFNLSEFEPRMDPVPVLGEHTRAILSELGYSGDEVESLAAAGAL"
BASE COUNT          616 a         1189 c         1234 g          591 t
ORIGIN      
        1 ggagcgcggg aggacgcgcg actgacgcca ttgcgccact tgcgggagct ttgatgaacc
       61 gatcgatcga gcgtacgttg caccgagccg ccgcgacgat tgccgcgacg atcgtcgcca
      121 cggcagcaac cgcagcggct gccgccggcg tgtcgtgtca cgcgcaaagc ggaccccgaa
      181 ccgcggcgct gctcgagctc tatacgtcgg aaggttgcga cagctgtccg ccggccgacc
      241 ggtggctgtc ggggcttggc gcgcgcggct atgcgccggg aagagtggtc ccgcttgcgc
      301 tgcacgtcga ttactgggat tacatcggct ggaaggatcc ctacgccagg cgcgaatttt
      361 ccctgcgcca gaggaagctc agccagctgc agcgcttggc gctcgtctat acgccgcaag
      421 tgctgctgca gggacgcgat ttcagggact ggggaggagc tgccttcgag acagccgtga
      481 gccgcatcaa cgcgcaaccc gccagggcgc gcatcgccct cgaaatccac tctctcgagg
      541 tgagccgcct cgacgtggcg gtgagcgcag agctgctcga cctttcccat cagggcgatg
      601 ccgtggttta tctcgctgcc tacgagaacc ggctcgtgag ccgcgtgacc gccggggaaa
      661 accgcggccg cacgctggga cacgactacg tggtgctcga atggcagggg ccgttcgcct
      721 tgggcccgcg cctcgcggag cggcgcgcgc ttcccctcct gcccgcgggc gtgccggcca
      781 actccggcgt ggtcggattc gtgcaaaacc gccgcaccgc cgaggtgctt caggctctca
      841 tgcttcccgc ctgcaagggc tagttcgggt atagttcgcg cccaataagc atccgccccg
      901 gtgggcgggg cgatgcgccc gcccgggggg cgggggttac tgaggagcac ccatgcaagc
      961 gacgcagatc cggcaaaagg acggcgtctt ctatttcgtc agctaccgcg ccaaggacct
     1021 gctggcgaag gtgcgtttca tcagccgctt ctacggggag ggagaggaga tcgccccggg
     1081 ccgcattgca caggacgacg acatcgccca gttcatcgcc aggatcgagc gcaacgatca
     1141 cgccttccag cgctcgctgt cgcgttccaa ggtgcgccag ttgaagaact tctacgagac
     1201 ggcgatcacg cagccgccga ttcccgggac ggtgctgctg ttcacctccg agcgattgag
     1261 cttccgtgcg ggcggcgagg ggggcggcgc aggcacgctc aacgagccgt cgtccaagta
     1321 cctgatcatc gacggccagc atcggctcgc cgcgctgcag ttctacctgc acgagcgccc
     1381 ggacgatgcg gcgaacatca gcgtgccctg catcctgttc gacggccgca gcgaggattt
     1441 cgccaccgag atgttcgtca tcatcaactc gacgcctacg cgcatcaaca agagccacct
     1501 ggtcgatctc tacgagcacg tgtcgttcgc cgcgccggat cgcaagttcg ccgcgcgcct
     1561 ggtcgagcgg ctctacagcg agggcgacag cccgctgcgc taccgcatca atcggctcgg
     1621 cgggcgcagc cagcgcgaca agtgggtgct gcaagcggag ctcttcaacg agctgcaccg
     1681 ctgggtgcgc ggacgctggc gctcgatcca gctcgccggc ggcagctcca aggaggtgcc
     1741 gcgctactac gcgatcgtgc gcgatttttt caaggcggcg cgcgcggtgt tcggcgacgc
     1801 ggtgtggggc aaggacaact acatggtcac caagcccgtg accatcaagg cgatggtacg
     1861 ggtatgcgcc gacctcgcgc gcgatgacgc cgagcccgag gccggccggg tcgcccgatg
     1921 ggaacagcgg ctcggcccct gggccgagat ggcgcggcaa ttccgcgagg aaggcttcta
     1981 cgagcgcttc ccggccaagg gcgaagtgga gcgcgtggcg cgcgttcacc gcgacctcgc
     2041 gcgggccgcg ggaatcgaat cgagccgtcc caagccgtaa ttcgccgccg gaataggcgc
     2101 gcgccgcagc tgttcgcgga ggtatgaaaa ccccactgtt actagctttc gccagcctga
     2161 caatggccgg ctgcgctggc atggacgcgg gcgagtgccg cagcgccaac tggtacgatc
     2221 ttggcttccg ggatgccatt ttcggaatgc agcgcatgga tgaggtttat gcgagccagt
     2281 gcggcacgca cggcgccgag cccgaccggg tccgctacgc gcaggggtgg cacgagggag
     2341 tctatgaatt cgaccagcgc aaagcccatg ggggacacga ctaaggcgct cgtcactgcc
     2401 gcctgcctgc ttgtcggcgg ctgcacagcc atgagcgaat ccgagtgccg cggcgccgac
     2461 tggtatcagc tgggctaccg cgatgcagac ccctacgggc tgcgcccgca gatcgacctt
     2521 tatgcccacc aatgccgcgc ctgggtgcag gcttccgaga gcgaatacat gcggggctgg
     2581 atagacgggt accgcgagtg gaatacgcgc gtccacggta gcgagtgctg cggcgcgcgc
     2641 tagagcgctc cggcggcagc cagcgattcg acctcgtccc cgctgtagcc cagttccgaa
     2701 agaatcgcgc gcgtgtgctc gccgagcacg ggaacggggt ccatgcgcgg ctcgaactcc
     2761 gacagattga acggcggctt gagcgcatcg agctcacccg ccggcgagcc gaccttcgcc
     2821 cagcgccgcc gcgcctcaag ctgcgggtgg cgccagaatt cgtgcattga atttaggcgc
     2881 gcgttcgcaa catccgcagt ttccagccgc tcgatgactt cctgggtttt catgtttgca
     2941 aaggcctggt ctatgtgcgc atgcagcgca gcgcggttct gcagccgggc cgggccggac
     3001 gagaacctgg ggtcggcggc cagggacctg tcctgcagca cgatgtcgca aaagcggccg
     3061 aattcccgtt cgttctggac gctgaggaac accgtgccgc cgtccccgac ccggaacggg
     3121 ccatacggcg cgatcgtggc gtgatgcgcc cctgaacgcg gcggcggcgt acccccgtag
     3181 gcggtgtagt acgcggggaa ccccatccat tcgccgagcg cctcgaacat cgtgatatcg
     3241 agcgtggcgc cctgaccgtc gcggcttcgc cggtacaacg ccgccaggat cgcggagaag
     3301 gcgtacatgc cggcggcgat atcggcgacc gggatgccgg ccttggaagg cgcttcggga
     3361 gtaccggtga ttgaaagcat gccggcttcg cactgcacca gaaggtcgta ggctttcttt
     3421 tcggcgtaag gaccggcggg accgtagccc gagataccgc accagatgag ccgtggatgc
     3481 ctagcccgaa gctctgctgc gccgagaccg agtcgctccg ccgcgccggg ggcaaggttt
     3541 tgcaggaaga tatcggcctt gctgagcagc ttttccagga tttgcttcgc cgcgggatgc
     3601 ttcacatcga gggccaggct ctccttcgag
//
LOCUS       DASPXW010000213         2504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1700885, whole genome shotgun sequence.
ACCESSION   DASPXW010000213 DASPXW010000000
VERSION     DASPXW010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1700885"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VEL04_05010"
     CDS             complement(<1..452)
                     /locus_tag="VEL04_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HYT47756.1"
                     /translation="MPEPSTDTEVLIVGAGPVGLTLAIDLGQKGVRCTLIERKEAPQF
                     LPKMERCNARTMEIFRRMRLAGKIRAAGLGREVPMDVYVILAMNQPPLLRLPYPSVAQ
                     ARREIDACTDGSMPLEPYQLISQYTLEPLLKSVAEKLPGVSVRYSCEFL"
     gene            512..1228
                     /locus_tag="VEL04_05015"
     CDS             512..1228
                     /locus_tag="VEL04_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47757.1"
                     /translation="MNNIRSWRFWLIIFLAIALAACAGRRERREEAKQERLERREQAR
                     AEREGRATPAAPATAPATAPAAPGAAPAPATAGDASAKMVFMRVGKMGGAISASVFDV
                     TEPGEPKFIGIVRQSSKVAYPVKPGLYTFMVISEAADFMQAAVVGGKTYYALVTPRMG
                     VWKARFSFQPVRGEEIGTKKFAGWERGTRLVTNTPDSLAWAKNNAADIADKRTRYWPE
                     WSGKSEKDRAAQTLNADDGR"
     gene            1241..2308
                     /locus_tag="VEL04_05020"
     CDS             1241..2308
                     /locus_tag="VEL04_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HYT47758.1"
                     /translation="MGPETQGFLKNCWYVAAWDHELVDGKLMPRTILEQPLVLYKGDS
                     GKAVALDNRCAHRGAKLSNGRLEGDCVRCMYHGLKFDASGKCVQIPGQDNIPPKLGVR
                     SYPVFERDHLIWIWMGNPALADPAKILDFPYLRDPAWRGKPDYMHYKANYLLIVDNLS
                     DFAHLAFVHTRTLGGSEEYAYQSKPTAIERLEDGFRVERWHMNADVPPFHNKIVSNAG
                     KVDRRNIARMHVPGIFFMETLFSPAGSGAEKGNLEGAKQYRNCQFFTPETRRSTHFFW
                     DYLHDYDLHDPAIALSLRDSMVQGFLEDKFIIEGQQELLDADPDFKMNGIVADAPLAH
                     FRRTLGKLIEAERLASQPVAA"
     gene            2322..>2504
                     /locus_tag="VEL04_05025"
     CDS             2322..>2504
                     /locus_tag="VEL04_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47759.1"
                     /translation="MRITLLALLFVSSALHAQTALTGLVSSAEEGAMEGVLVGAKKAG
                     STITVTVVSDAQGRYSF"
BASE COUNT          479 a          795 c          834 g          396 t
ORIGIN      
        1 aggaactcgc agctgtagcg cacgctcacg ccgggcagct tctccgccac cgatttgagc
       61 aggggctcga gggtgtattg ggaaatgagc tgatagggct cgagcggcat cgagccgtcg
      121 gtacaggcgt caatctcgcg ccgcgcctgt gccaccgacg gatacggcag ccgcaacaac
      181 ggcggctggt tcatcgccag gatcacatag acgtccatcg gcacttcgcg cccgagcccg
      241 gcggcgcgga tcttcccggc gagcctcatg cgccggaaaa tctccatggt gcgggcgttg
      301 cagcgctcca tcttgggaag gaactgcggc gcttccttgc gctcgatcag cgtgcagcgc
      361 acgcccttct ggccgaggtc gatggcgagc gtcagtccga ccggcccggc gccgacgatc
      421 agcacctcgg tatctgttga cggctcaggc atgggttcgg atgataatcg ccaaaactaa
      481 gaggcataat ccgcaaaagg gggaacgttc aatgaacaac atcagatcgt ggcgtttctg
      541 gctcattatt ttcctggcga ttgcccttgc agcctgcgcg ggacggcgcg aacggcgcga
      601 ggaggcgaaa caggagcggc tggagcggcg cgagcaggcg agggcggagc gggagggacg
      661 ggcgacgccc gcggcgccgg ctacagcgcc ggccactgcg cctgcggcac cgggcgcggc
      721 gcctgcgccg gcaacagcgg gggacgcctc ggccaaaatg gtattcatgc gcgtcggcaa
      781 gatggggggc gcgatttcgg cttcggtctt cgacgtgacg gagccgggcg agccgaagtt
      841 catcggtatc gtcaggcaaa gcagcaaggt cgcgtacccg gtgaagccgg gcctgtacac
      901 cttcatggtc atcagcgaag cggcggattt catgcaggcg gccgtggtcg gcggaaagac
      961 ctactacgcg ctggtcacgc cgcgcatggg agtgtggaag gcgcgctttt cgttccagcc
     1021 cgtgcggggc gaggagatcg gcacgaagaa gttcgcgggc tgggagcggg gcacgcgcct
     1081 cgtcaccaac acgccggatt cgctggcctg ggcgaagaac aacgcggccg acatcgcaga
     1141 caagcgcacc cgctactggc ccgagtggag cggcaagtcc gaaaaagacc gcgccgcgca
     1201 gacgctcaat gccgacgacg ggcgctgacg gaggaccgat atgggacctg agacgcaagg
     1261 cttcctgaag aactgctggt acgtcgcggc ctgggaccac gagctggtcg acggcaagct
     1321 gatgccgcgc accatcctcg agcagccgct cgtgctctac aagggcgaca gcggcaaggc
     1381 ggtggcgctc gacaaccgct gtgcccaccg cggtgccaag ctttccaacg gccgcctgga
     1441 aggcgactgc gtgcgctgca tgtaccacgg cctgaaattc gatgcctcgg gcaaatgcgt
     1501 gcagatcccc gggcaggaca acatcccgcc gaagctcggc gtgcgcagct accccgtgtt
     1561 cgagcgcgac catctcatat ggatctggat gggcaacccg gcgctcgccg atccggcgaa
     1621 gatcctcgac tttccctatc tgcgcgatcc ggcctggcgc ggcaagccgg actacatgca
     1681 ttacaaagcg aactacctgc tgatcgtcga caacctgagc gacttcgcac acctcgcctt
     1741 cgtgcacacc aggacgctcg gcggctcgga ggaatacgcg taccagtcca agccgaccgc
     1801 gatcgagcgc ctcgaagacg gcttccgggt cgagcgctgg cacatgaatg ccgatgtgcc
     1861 tccgtttcac aacaaaatcg tcagcaatgc aggaaaagtc gaccggcgca acatcgcgcg
     1921 catgcacgtg ccggggatct tcttcatgga gacgctgttc tcgcccgcgg gctcgggcgc
     1981 cgagaaaggc aacctcgagg gcgcgaagca ataccgcaac tgccagttct tcacgcccga
     2041 gacgcggcgc tcgacgcatt tcttctggga ctacctgcac gactacgacc tgcacgaccc
     2101 ggccatcgcg ctgtcgctgc gcgacagcat ggtgcagggc ttcctcgagg acaagttcat
     2161 catcgagggt cagcaggagt tgctcgacgc cgacccggac ttcaagatga acggcatcgt
     2221 cgcggacgcg ccgctcgcgc acttccgcag gacgctcggc aagctgatcg aggccgagcg
     2281 cctggcgtca caaccggtcg ctgcctgaat aggagagcgt catgcgaatc acgctgcttg
     2341 cgctgctgtt tgtgtcttca gcccttcatg ctcagacggc gttgaccggc ctggtgagct
     2401 ccgccgaaga gggcgcgatg gagggtgtgc tcgtcggcgc gaagaaggcg ggttcgacga
     2461 tcaccgtgac cgtcgtcagc gacgcgcagg gccgctacag cttc
//
LOCUS       DASPXW010000214         3622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_64856, whole genome shotgun sequence.
ACCESSION   DASPXW010000214 DASPXW010000000
VERSION     DASPXW010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64856"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..986
                     /locus_tag="VEL04_05030"
     CDS             12..986
                     /locus_tag="VEL04_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYT47760.1"
                     /translation="MERLLYEVKKMVVGQDHFLERVLVAVLAQGHLLVEGVPGLAKTL
                     TVKTLAAALRGSFRRIQFTPDLVPADLVGTRIYNQKTGEFSTSLGPVFCNLLLADEIN
                     RAPAKVQSALLEVMQERQVTIAGETHRVPEPFLVMATQNPIETEGTYALPEAQVDRFM
                     MKVLVDYPSEEEEFVIVERVTGDIHETAAVATMEQLGALQRECRKAYVEPALIQYAVR
                     LAAATRDPERCGIKGIEKYLAYGASPRAPIHVIEGARALAFLRGRDYALPQDVTDLVP
                     DVFRHRLVLSYEALAEGLTPDALIGRIMQHVRAPEKPLESHVSVAAGS"
     gene            961..1875
                     /locus_tag="VEL04_05035"
     CDS             961..1875
                     /locus_tag="VEL04_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HYT47761.1"
                     /translation="MSALPQDPERILRRLEWTALRRLDGLLQGDYRTLFRGFGLDLAD
                     LREYQYGDDVRHIDWNVTARLQTPYVREYHEDREITAWFLLDLSGSVDFGSREVKKQS
                     VSAEFVGVLARILTRRGNRVGALMYGSAVDTVIPARSGRRQVLQILHRMMGRRENPGN
                     TMTDLRELLDAAAHLMRRRSLVFVVSDFFSAPGWTGLMGELAQRHEVVAVRLHDPIEA
                     ELPDLGLLMFRDAETGEQLFVDTHDRGFRSRFAAAAERREANLRAALRDAGVDAMELA
                     TDADLVDEIRRFVELRKRRSHSTARAAH"
     gene            1872..2894
                     /locus_tag="VEL04_05040"
     CDS             1872..2894
                     /locus_tag="VEL04_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYT47762.1"
                     /translation="MSFQWPEMLWLMLALPLLVAAYFVALRRKKKLALRYASLSLVKE
                     ALSAGQTFRRHVPPLLLLVALAIMIAAVARPGAVVTLPSQHETVILAMDVSGSMRATD
                     VEPNRLVAAQVAAKAFVAQQPENVRIGVVAFAGTATLAQAPTRNREEVIAAIDRFQMQ
                     RATAIGSAIIVSLATIFPDHGLDVASFTYGAEAARKTQKQTAFKPVPPGSYQSAVIIL
                     LTDGQRTTGPDSVQAARLAAERGVRIYTVGVGTPGGATIGFEGWSMHVRLDEETLKTI
                     ADLTRGEYFYAGTAVDLKKIYEGMRSRMVLETKKIEVTALFAAAGALLVAVAALLSLA
                     WFNRIL"
     gene            complement(2899..3075)
                     /locus_tag="VEL04_05045"
     CDS             complement(2899..3075)
                     /locus_tag="VEL04_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47763.1"
                     /translation="MKRKYGAALARETIAEQHMRERAKEAGNNRDNFGSLSRKGAVSR
                     GKLRGRAYRAGRRK"
     gene            3325..3552
                     /locus_tag="VEL04_05050"
     CDS             3325..3552
                     /locus_tag="VEL04_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47764.1"
                     /translation="MVKTISKIGNSQGLIFDTALREITGLKAGDEVNITVHEGGAVVI
                     TPMRSEIPPQESGRAARKLIRRNTALFKRLA"
BASE COUNT          589 a         1226 c         1223 g          584 t
ORIGIN      
        1 tctcggcacc gatggaacgc ctgctctacg aggtcaagaa gatggtcgtc ggccaggatc
       61 atttcctgga gcgcgtgctg gtcgcggtgc tcgcgcaggg ccacctgctg gtcgaaggcg
      121 tccccggtct cgccaagacg ctcaccgtga aaaccctcgc cgcggcgctg cgcggctcgt
      181 ttcgccgcat ccagttcacg cccgacctgg tgcccgccga cctggtcggc acgcgcatct
      241 acaaccagaa gaccggcgag ttctccacct cgctcggccc ggtgttctgc aacctgctgc
      301 tcgccgacga gatcaaccgc gcgccggcca aggtgcagag cgcgctgctc gaggtgatgc
      361 aggagcgcca ggtcacgatc gcgggcgaaa cgcaccgcgt gcccgagccg ttcctggtga
      421 tggccactca gaatccgatc gaaacggaag gcacctacgc gctgcccgag gcgcaggtcg
      481 accgcttcat gatgaaggtc ctggtggact acccgagcga ggaggaggag ttcgtcatcg
      541 tcgagcgggt caccggggac atccatgaaa ccgccgccgt cgcgacgatg gaacaactgg
      601 gagccctgca gcgcgagtgt cgcaaggcgt atgtcgagcc cgcgctgatc cagtacgcgg
      661 tgcggctcgc cgccgcaacg cgcgatccgg agcgttgcgg catcaagggc atcgagaaat
      721 atctcgctta cggcgccagc ccgcgcgccc cgatccacgt catcgagggc gcgcgcgcgc
      781 tcgccttcct gcgcgggcgc gattatgcgc tgccgcagga cgtgaccgac ctggtgcccg
      841 acgtcttccg ccatcgcctg gtgctgtcct acgaggcgct cgccgaagga ctgacgcccg
      901 atgccctgat cgggcgcatc atgcagcacg tgcgcgcgcc cgagaaaccc ctcgagagcc
      961 atgtcagcgt tgccgcagga tcctgagcgg attctccgcc ggctggagtg gacggcgctg
     1021 cgccggctcg acggcttgct gcagggcgac taccggaccc tgttccgcgg cttcggcctc
     1081 gacctcgccg acctgcgcga ataccagtac ggcgacgacg tgcgccacat cgactggaac
     1141 gtgaccgcgc ggctgcagac gccctacgtg agggaatacc acgaggaccg cgagatcacc
     1201 gcctggtttc tgctcgatct gagcggctcg gtggatttcg gctcgaggga ggtgaagaaa
     1261 caatcggtat ccgcagaatt cgtcggcgtg ctcgcgcgca tcctgacgcg ccgcggcaac
     1321 cgcgttggcg ccctgatgta cggctcggca gtcgatacgg tgatcccggc gcgcagcggg
     1381 cggcgacagg tgctgcagat cctgcaccgc atgatgggac gccgcgaaaa tcccgggaac
     1441 acgatgaccg acttgaggga gctgctggac gcggccgcgc acttgatgcg caggcgctcc
     1501 ctagtattcg tcgtgtccga tttcttcagc gctccgggct ggaccgggct catgggcgag
     1561 ctcgcgcagc gtcacgaggt tgtcgccgtg aggctgcacg acccgatcga ggccgagctg
     1621 cccgacctgg ggctgctcat gttcagggac gccgagaccg gcgaacagct gttcgtcgat
     1681 acgcacgacc gcggcttccg cagccgcttc gccgcagccg ccgagcgtcg cgaggcgaac
     1741 ctgagggcgg cgttgcgcga tgccggcgtc gatgcgatgg agctcgccac cgacgccgac
     1801 ctggtggacg agatccgccg cttcgtcgag ctgcgcaagc ggcgcagcca ttccaccgca
     1861 agggccgccc attgagcttc caatggcctg aaatgctctg gctgatgctc gcgctgccgc
     1921 tgctggtggc ggcctacttc gttgcgctgc gccgcaagaa gaagctggcg ctgcgctacg
     1981 ccagcctgag cctggtgaag gaggcgctga gcgccggcca gacgttccgg cggcacgtcc
     2041 cgccgctgct gctgctcgtc gcgctcgcca tcatgatcgc cgccgtggcg cgtcccggcg
     2101 cggtggtcac gctgccgtcg cagcacgaaa ccgtgatcct ggcgatggat gtctccggca
     2161 gcatgcgcgc aaccgatgtc gagcccaacc ggctggtcgc cgcgcaggtc gccgcaaagg
     2221 ccttcgtcgc ccagcagccc gagaacgtgc gcatcggcgt ggtggctttc gccggcacgg
     2281 cgacgctcgc gcaggcgccg acgcgcaacc gcgaggaggt gatcgccgcc atcgaccgct
     2341 tccagatgca gcgcgccacc gccatcggca gcgcgatcat cgtctcgctg gcgacgattt
     2401 tccccgacca cggcctcgac gtggcgtcct tcacctacgg cgccgaggcc gcgcgcaaga
     2461 cgcagaagca gaccgcgttc aagccggtgc cgccgggctc gtatcagtcc gcggtcatca
     2521 tcctgctcac cgacggccag cgcaccaccg gccccgactc ggtgcaggcg gccaggctcg
     2581 ccgcggagcg cggcgtgcgt atctataccg tgggcgtcgg cacgcccggg ggcgcgacca
     2641 taggcttcga gggctggtcg atgcatgtgc gcctggacga ggagacgctg aagacgatcg
     2701 ccgacctgac gcgcggcgag tacttctacg caggcaccgc ggtggacctg aagaaaatct
     2761 acgagggcat gcgctcgcgc atggttctgg aaacgaagaa aatcgaagtg acggcgctgt
     2821 tcgccgctgc cggcgcgctc ctggtggcgg tggcggcgct gctttcgctc gcctggttca
     2881 atcgcatcct gtaggccgct acttgcgtcg cccggcgcgg taggctctgc cgcgcagctt
     2941 gccgcgcgaa actgcgccct tgcgcgacaa tgagccgaag ttgtccctgt tgttgccggc
     3001 ctctttggcg cgttcccgca tatgctgctc ggcaatggtt tcccgcgcca gcgcggcgcc
     3061 gtatttgcgt ttcatggatt ctccagacga ggggcgcaat agtaccgtga aagcgcctgg
     3121 tggccgcggc tctcccgccg caatcgcgag aagctgcgcc acctcggcag ggtcttcccg
     3181 cgcgagcgcc tctcggtggc cgagccgctc gcgccggcgg ccgcgacgcc cgagcggctg
     3241 cgcgcggcgg tgctggagct gcgcggctcg tggaagcagc cggcccgcat tgcaggcatg
     3301 taaaacattg tttaacatag agggatggtc aaaaccatct ccaagatcgg caattctcag
     3361 ggcctcattt tcgacacggc gctacgcgag atcaccggac tcaaggcggg cgacgaagtg
     3421 aatatcacgg tgcacgaagg gggggcggtg gtcattacgc cgatgcgcag tgagattcct
     3481 ccccaggaga gtggccgcgc cgcacgcaag ctgatccggc gcaacacagc gcttttcaag
     3541 cgccttgcgt aggcgaagct atcggcacct cggagcggag gcagtcgagg caattcaccg
     3601 ggaggtgctt gccgcgcatg gt
//
LOCUS       DASPXW010000215         2108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_188629, whole genome shotgun sequence.
ACCESSION   DASPXW010000215 DASPXW010000000
VERSION     DASPXW010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188629"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VEL04_05055"
     CDS             <1..235
                     /locus_tag="VEL04_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase"
                     /protein_id="HYT47765.1"
                     /translation="TCTGDHSCIRLSGCPTLTVKDNPDPLRTDPVATVIDGCVGCGLC
                     GENAHAAVLCPSFYRAEVIQNPTVWDRLVCRIL"
     gene            232..1692
                     /locus_tag="VEL04_05060"
     CDS             232..1692
                     /locus_tag="VEL04_05060"
                     /inference="COORDINATES: protein motif:HMM:NF042682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta
                     family protein"
                     /protein_id="HYT47766.1"
                     /translation="MNLPERPITVLIAALGGEGGGVMADWLMEAATASGYPAQATSIP
                     GVAQRTGATTYYLEIYPEKKDKLNGKDPVLSLTPSPGNVDVMVASELIEAGRAMQNGF
                     VSPERTTLIASTHRIYATIEKMQMADGRFDSERVLDAAKQLTKKAVLFDMRKLAEESG
                     TVINAVLFGAMAGSGVLPLARAACESAIRGAGRGAEASLRGFAAGYDIAAGTSHPPQP
                     AAPPRRATEVNEILQLGRERLKDYQGERYAALYVERMGPFLGGEAKLAAEVGRHLATW
                     MSYEDIIRVADLKTRAARFERIRKEVSAKSGEPVVVIDYLKPGVEEFASILPAFLGRK
                     LVSWAERRGKLDAYNVGMHIKTSGILGYLLVRSLAWLKPWRPRSYRYQEEQQLIGRWL
                     GHVAEAAKRDAGLALEVALCAKLVKGYGETHRRGKGNFLAIVDALIENPATADAREQA
                     RAIRKAREAALADPEGQALEKQLGKPVVWLKPVSRT"
     gene            1705..>2108
                     /locus_tag="VEL04_05065"
     CDS             1705..>2108
                     /locus_tag="VEL04_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYT47767.1"
                     /translation="MNAPDKAFLAREYNNRELVPEHPQFFARWGEASLRARSRMLSYL
                     DRRYGDMPGETIDLFPARKGDGTCMMFIHGGYWRSLDKQDFSFLAPAWVDAGVSLAVV
                     NYDLCPKVTMEEIVRQMLRASRWLWLHAEQHG"
BASE COUNT          394 a          698 c          700 g          316 t
ORIGIN      
        1 cacctgcacc ggggaccact cgtgcatccg gctctcgggc tgcccgacgc tcaccgtcaa
       61 ggacaacccg gacccgctgc gcaccgaccc ggtggcgacc gtgatcgacg gctgcgtcgg
      121 atgcgggctg tgcggcgaga atgcgcacgc agccgtcttg tgcccttcgt tttacagggc
      181 agaagttatc cagaatccaa ccgtctggga ccgattggtt tgcagaattc tatgaacctt
      241 ccagagcgcc ccatcaccgt cctgatcgcc gcgctcggcg gcgagggcgg cggcgtgatg
      301 gccgactggc tgatggaagc cgccaccgcg agcggctacc cggcgcaggc cacctcgata
      361 ccgggcgtcg cccagcgcac cggggcgacc acgtattacc tggaaattta tccagagaaa
      421 aaagataagt taaacggcaa agatcccgtt ctctcgctga cccccagtcc cggcaatgtc
      481 gacgtcatgg tcgcctccga gcttatcgag gccggccgcg cgatgcagaa cggcttcgtc
      541 agccccgagc gcaccacgct catcgcctcg acgcatcgca tctacgcgac catcgagaag
      601 atgcaaatgg ccgacggccg cttcgacagc gagcgcgtgc tcgacgccgc gaagcagctg
      661 acgaaaaagg cggtgctctt cgacatgcgc aagctcgccg aggagagcgg tacggttatc
      721 aatgccgtgc tgttcggcgc catggcgggc agcggcgtgc tgccgctcgc gcgcgcggcc
      781 tgcgagagcg cgatccgcgg cgccggccgc ggcgccgagg cgagcctgcg cggcttcgcc
      841 gccggctacg acatcgcggc cggaacgagc cacccgccgc aaccggcggc gccgccgcga
      901 cgggcaaccg aggtcaatga aattctccag ctgggaagag aaagattgaa ggattaccag
      961 ggcgagcgtt acgccgcgct ctatgtggag cggatgggac cgtttctcgg cggcgaggcg
     1021 aagctcgccg ccgaggtcgg ccgccacctc gcaacctgga tgagctacga ggacatcatc
     1081 cgcgtcgccg acctgaagac gcgcgctgcg cggtttgaaa ggatcagaaa ggaagtatca
     1141 gcaaagagcg gcgagccggt ggtcgtcatc gactatctca agccgggtgt cgaggagttc
     1201 gcctcgattc ttcccgcctt tctcggcagg aagctcgttt cctgggccga gcggcgagga
     1261 aagctcgacg cctacaacgt cggcatgcac atcaagacct cggggatcct cggctacctg
     1321 ctggtcagga gcctcgcctg gctcaagcca tggcggccga ggagctaccg ctaccaggaa
     1381 gaacagcagc tgatcgggcg ctggctgggc cacgtcgccg aggcggcaaa gcgcgacgcc
     1441 ggcctggcgc tcgaagtcgc actgtgcgcc aagctggtca agggctatgg cgagacgcac
     1501 cggcgcggca agggcaattt cctcgccatc gtcgacgccc tgatcgagaa cccggcgacg
     1561 gcggatgcgc gcgagcaggc cagggcgatc cgcaaggcgc gcgaggcggc gctagccgat
     1621 ccggagggcc aggcgctgga gaagcagctc ggcaagccgg tggtgtggct caagccggtc
     1681 agcagaacgt aaaatcccac ctttatgaac gcgcccgaca aggccttcct cgcgcgggaa
     1741 tacaacaacc gcgagctggt tcccgagcat ccgcaattct tcgcccgctg gggcgaagcg
     1801 tcgctccgcg cccgcagccg catgctgagc tacctcgacc gccgctacgg cgatatgccg
     1861 ggagagacca tcgatctgtt cccggcgcgc aagggtgacg gcacctgcat gatgttcatc
     1921 cacggcggct actggcgctc gctcgacaag caggacttct cgttcctcgc cccggcatgg
     1981 gtcgacgccg gcgtgtcgct cgcggtggtg aactacgacc tctgcccgaa ggtgacgatg
     2041 gaggagatcg tgcgccagat gctgcgcgcg agccgctggc tgtggctgca cgccgagcag
     2101 cacggcat
//
LOCUS       DASPXW010000216         2403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1072791, whole genome shotgun sequence.
ACCESSION   DASPXW010000216 DASPXW010000000
VERSION     DASPXW010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072791"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1841)
                     /gene="aceE"
                     /locus_tag="VEL04_05070"
     CDS             complement(<1..1841)
                     /gene="aceE"
                     /locus_tag="VEL04_05070"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013689713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HYT47768.1"
                     /translation="MANLSDIVTSAFWRVNPPDADPAETREWLEAFDALVETEGRERA
                     TFLLRKLLDHARARRVPLPPVLNTPYKNSVALADQPQFPGNLELESRISAMVRWNALA
                     MVVRANRAHAELGGHIASYASAADLFEVGFNHFFRGGQDGDLIYFQPHSAPGVYARAF
                     LEGRLSQENLANYRRETGGKGLCSYCHPYLMPDFWQFPTGSMGLGPINAIYQARFMRY
                     LEHRGIAGRAPETAERKVWAFVGDGEMDEPESLAGLSLAARDGLDNLIFVVNCNLQRL
                     DGPVRGNGSIVQELEGLFAGAGWNVLKLLWGSDWDALFARDTDGVLLKRLHETVDGEF
                     QTYAATDGRFNREHFFNKYPELQRLVAHLSDDDIDRLRRGGHDPVKIHAAYRAAVEHK
                     GQPTVILAKTKKGYGMGLWGQGKMGTHQQKKPDDDALREFRDRFLLPLSDDDVAQLRF
                     YHPGADSPEIRYLEARRKALGGYLPARRRAAPRLAAPALQGSPRSQSTTMVFVQLLSQ
                     LMKDEQLGPRVVPIVADEARTFGMQTLFRQFGIYSALGQLYEPEDHEELLYYREAKDG
                     QILEEGITEAGALSSWLAAATAYSAHGTPMLPFYIYYSMFGFQRVGDL"
     gene            2039..2242
                     /locus_tag="VEL04_05075"
     CDS             2039..2242
                     /locus_tag="VEL04_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004196837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HYT47769.1"
                     /translation="MATGTVKWFNDSKGFGFITPTEGGEDLFAHHTAIQMPGFKTLKE
                     GQKVEFEVKRGPKGLQAANIKPI"
BASE COUNT          440 a          765 c          779 g          419 t
ORIGIN      
        1 aggtcgccga cgcgctggaa accgaacatc gagtaataga tgtagaacgg cagcatcggc
       61 gtgccatggg ccgagtacgc agtcgcggcg gcgagccacg atgacagcgc gcccgcttcg
      121 gtaatgccct cctcgagaat ctggccgtcc ttcgcctcgc ggtagtagag cagctcctcg
      181 tggtcttcag gctcgtagag ctggccgagc gcggagtaga tgccgaactg ccggaacagc
      241 gtctgcatgc cgaaggtgcg cgcctcgtcg gcgacgatcg gcaccacgcg aggcccgagc
      301 tgttcgtcct tcatgagctg cgagagcagc tgcacgaaca ccatggtggt cgactggctg
      361 cgcggcgagc cctgcagcgc cggtgcggca agccgcggcg ccgcgcgcct gcgtgccggc
      421 aggtagccgc ccaaggcctt tcttcttgcc tccagatacc tgatttccgg gctgtcggct
      481 cccgggtgat agaagcgcag ctgcgccacg tcgtcgtcgg agagcggcag caagaaacgg
      541 tcgcggaact cgcgcagcgc gtcgtcgtcg ggcttcttct gctggtgcgt gcccatcttc
      601 ccctggcccc acagccccat gccatagcct ttcttggtct tggcgaggat caccgtcggc
      661 tggcccttgt gctcgaccgc cgcgcggtag gcggcatgga tcttcaccgg gtcgtggccg
      721 ccgcggcgca ggcgatcgat atcgtcgtcg gagagatgcg ccaccaagcg ctgcagctcc
      781 gggtacttgt tgaagaaatg ctcgcggttg aagcggccgt cggtcgcggc gtaggtctgg
      841 aattcgccgt ccaccgtctc atggaggcgc ttcagcagca cgccgtcagt gtcgcgcgcg
      901 aacagcgcat cccagtccga gccccacagc agcttgagca cgttccagcc ggcgccggcg
      961 aacagccctt cgagctcctg cacgatcgag ccgttgcctc gcaccgggcc gtcgagccgc
     1021 tgcaggttgc agttgacgac gaagatcagg ttgtcgagac cgtcgcgcgc cgcgagcgac
     1081 agcccggcaa gcgactcagg ctcgtccatc tcgccgtcgc cgacgaaggc ccacaccttc
     1141 cgctctgcag tctcgggggc tcttccagct atcccgcggt gctcgaggta gcgcatgaag
     1201 cgcgcctggt agatggcgtt gatcgggccg aggcccatcg acccggtggg aaactgccag
     1261 aaatcaggca tcaggtacgg atggcaatac gagcacaagc ccttgccgcc ggtttctctt
     1321 cggtaatttg caagattttc ctgggacagg cggccctcca ggaaagccct ggcatagacc
     1381 ccgggcgccg aatgcggctg gaaatagatc agatcgccat cctgcccgcc gcggaaaaag
     1441 tggttgaagc cgacctcgaa caggtcggcc gccgaggcgt agctcgcgat gtggccgccg
     1501 agctcggcgt gcgcccggtt ggcgcgcacc accatcgcga gcgcattcca gcgcaccatc
     1561 gccgagatgc ggctctcgag ctcgaggttg ccgggaaact gcggctgatc ggcaagcgcc
     1621 accgaatttt tgtacggggt attgagcacc ggcggcagcg gcacgcgtcg cgcgcgggcg
     1681 tgatcgagca gcttgcgcag gaggaacgtg gcgcgctcgc gcccttcagt ctcgaccagt
     1741 gcgtcgaagg cttccagcca ttcgcgcgtt tccgcggggt cggcgtcggg cggattcacg
     1801 cgccagaacg cggaggtgac gatgtctgag aggttggcca tgccggaatg gtagccctcg
     1861 acggcaaggc gcggaaaaag ctgatatgat gcggcccgca ggatattcag gcagcgaatt
     1921 tgtcggcttc tacctcagat ctcacccgta gcggcagttc agttccctcc ctcgatcgtc
     1981 gtgcccggct tggtcgcgcc cggaattgtc gcggcagtca gttgaaggaa atcagtaaat
     2041 ggcaaccggt accgtaaagt ggttcaacga ctccaagggc tttggcttca tcaccccgac
     2101 tgaaggcggc gaggatctgt tcgcgcatca caccgcgatc cagatgcccg gcttcaagac
     2161 gctgaaggaa ggccagaagg tcgagttcga agtgaagcgc ggccccaagg gcctgcaggc
     2221 ggccaacatc aagccgatct aagaaagcgt ttcgcccgga ctgaaagccc gctgcggcgg
     2281 gctttttcat ttctagggac aacccccgcc tacagggcga gccgcttcaa tcgctcatga
     2341 cgttcggaaa cagcatgccg aagctcagaa tgcacacggt aagcttgacg atgaacctaa
     2401 att
//
LOCUS       DASPXW010000217         5526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_316073, whole genome shotgun sequence.
ACCESSION   DASPXW010000217 DASPXW010000000
VERSION     DASPXW010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5526
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316073"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VEL04_05080"
     CDS             complement(<1..329)
                     /locus_tag="VEL04_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HYT47770.1"
                     /translation="MKILTVGAGPAGLYFAYLAKRQRPDWQIRVVEQNRADSTFGFGV
                     VFSDRALEFLRADDPQTYDLITPRMEIWADIAVVHRGTPVVIDGIGFAAIGRLQLLQL
                     LQRQLGSV"
     gene            complement(330..1415)
                     /locus_tag="VEL04_05085"
     CDS             complement(330..1415)
                     /locus_tag="VEL04_05085"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005085.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HYT47771.1"
                     /translation="MTPPFRADHIGSLLRPKQLREAFRKHASGEMPDGQFRAAQDEAI
                     RDAVKLQEDCGLQVVTDGEFRRISYWEKFVRLTKGLVVKDGVFRFHDERGHESDFTAP
                     YAEGKVSRLAPITLDEFGFVSQNTKQTAKITMPAPSTMHFYRFTDWGVYGDVREFFAD
                     LGKIYQAEIADLAAAGCRYVQLDEVAVAILCDPAARDKVKAKGADPERLVDLYIDAIN
                     QAVKHRPPQVTVGVHVCRGNYKGMYLSEGGYDSVAEKLFGRTDANHFLLEFDSPRAGG
                     FAPLRFVPKDKGVVLGLVSSKSSQLEKLDLLRKRADEAAKYVDAARLAISPQCGFAST
                     LGGNPVTEADERAKLRLCVDTAHAIWG"
     gene            complement(1472..2365)
                     /locus_tag="VEL04_05090"
     CDS             complement(1472..2365)
                     /locus_tag="VEL04_05090"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HYT47772.1"
                     /translation="MNVQDVDLNLLRVFDAVLAARGVTPAAVRLGLTQPAVSNALARL
                     RALFGDALFVRTPAGMDATPFARELAEPVRQALALLESALAHGPGFDASTSTRAFRFY
                     MSDLGQVEFLPPLVERAHREAPGVRFEAVALDVEDIDGALAAGALDLAVGFLPGLGPP
                     VRRRRLFRDPYVCLMRADHPIQALTKKKFVEASHALVSYRGGHRVIEEALERAGLARR
                     IALRVPHFTVVPMVLERSDLILTLPARVARVFERRGKFKSLPPPVPIPPADVAVHWHE
                     RFEADPGNRWLRELVIELLSD"
     gene            2450..3403
                     /gene="dctP"
                     /locus_tag="VEL04_05095"
     CDS             2450..3403
                     /gene="dctP"
                     /locus_tag="VEL04_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HYT47773.1"
                     /translation="MKKLLLIALAALVPSLAGAQETTLRVVSAFAENTQYVKNLEPFM
                     QKLNAEGKGSLQLNFIGGPKAMPPFEVGNAVRTGVVDVAMTTGAFYTNIMPEADALKL
                     TQIPATELRKNGGYELINRIWNEKANMQYLGRVIDFTPFHLYLTKKIDKPDLTGLKIR
                     ITPVYREFFQALGATVMQTAPGEVYTALERGVVDGYGWPINGIFDLNWHEKTKFRVDP
                     GFYNAEVSLVMNLDKWKSVTQPQRDLLMRHVIALEAQNDSWKKVNADDIKRQAQAGIQ
                     VISFDPATAKQYYNKAYEVGWASVIKASPQYGPEMRKLFSR"
     gene            3400..3855
                     /locus_tag="VEL04_05100"
     CDS             3400..3855
                     /locus_tag="VEL04_05100"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HYT47774.1"
                     /translation="MKLYYSLLKILAGAAALLLGAMALLVTLDVVARNVGLGTIAWVN
                     EVSEYSLPVATLLVAPWLLHRNEHVRLDVLLVLLPRQVAKILERCADLMGIAICAVFV
                     WYGAKLILDSARIDSMVVKTLAIPEWWQYALVPVCFFLLAVEFARRLRA"
     gene            3852..5168
                     /locus_tag="VEL04_05105"
     CDS             3852..5168
                     /locus_tag="VEL04_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HYT47775.1"
                     /translation="MTWWLSAIVLFGVLTAFFLMGLAAAFAFFGINIVGAWFYLEKEA
                     GLVQLIRNGIVSITSFSLTPIPFFILMGEVLFHTGVAMKAIDAIDRLIWRVPGRLSVV
                     AVLAGTVFSAISGSTIATTALLGSLLLPQMLARGYHPSMAMGPIMAIGGVDMLIPPSA
                     LTVLLGSLAGISISQLLIAGIVPGVLLSVMFVAWIVLRAMLDPSLAPAFEHAEVSPWE
                     RLKPLLLYVVPLVLIFVVVVAAMAAGWATPTEAAAIGAVATVAVAGVYRALTLPNLMQ
                     ALMGTAGISGIILFIIIGATTFSQILTFSGATNGLVEAIKQASFGPWTVLVAMMLILL
                     VLGCFIDQVSMMLICLPFFMPLVELYRFDPIWFGVLFLICMQLGLLTPPFGMLLFTMK
                     AVAPKEIEMRQVWHAVTPYVVMGLGALVAVILFPSLATWLPKVLFR"
     gene            5172..>5526
                     /locus_tag="VEL04_05110"
     CDS             5172..>5526
                     /locus_tag="VEL04_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase"
                     /protein_id="HYT47776.1"
                     /translation="MERSFKQEVAKLKLGDGETFHGEGILAVTKALLQSGVSYVGGYQ
                     GAPVSHVMDVLNDARDILDELGVHVEANANEAGAAAMLGASINYPMRGAVAFKSTVGT
                     NVASDALSNLASAGVK"
BASE COUNT          935 a         1803 c         1799 g          989 t
ORIGIN      
        1 acgctcccca gctggcgctg cagcagctgc agcagctgca gccggccgat ggcggcgaag
       61 ccgatgccgt cgatgaccac cggggtgccg cggtggacca cggcgatgtc ggcccagatc
      121 tccatgcgcg gcgtgatcag gtcgtaggtt tgcgggtcat cggcgcgcag gaattcgagc
      181 gcgcggtccg agaacaccac gccgaagccg aaggtcgaat cggcgcggtt ctgctccacg
      241 acgcggatct gccagtcagg acgctgccgc ttggcgagat aagcgaagta caggcccgcg
      301 ggcccggcgc ccacggtgag gattttcatc tagccccaga tggcgtgcgc cgtgtcgacg
      361 cacaatctca gcttcgcgcg ctcgtcggct tcggtgaccg ggttgccgcc cagggtgctg
      421 gcgaagccgc actgcgggct gatggcgagg cgcgcggcgt cgacgtactt tgccgcctcg
      481 tcggcccttt tcctgagcaa atccagcttc tccagttgag aagacttcga gctcaccagc
      541 ccgagcacca cgcccttgtc cttcggcacg aagcgcagcg gcgcgaagcc gccggcgcgc
      601 ggcgagtcga actcgagcag gaagtggttc gcgtcggtgc ggccgaagag tttctccgcc
      661 accgagtcgt agccgccctc tgagaggtac atgcccttgt agttgccgcg gcagacatgc
      721 acgccgaccg tcacctgcgg ggggcggtgc ttcactgcct ggttgatggc gtcgatgtaa
      781 aggtcgacca ggcgctccgg gtctgcgccc tttgccttga ccttgtcgcg cgccgcggga
      841 tcgcacagga tcgcgaccgc gacctcgtcg agctgcacgt agcggcagcc cgccgcggcg
      901 agatcggcaa tttcggcctg gtagatcttg ccgagatcgg cgaaaaattc cctcacgtcg
      961 ccatacacgc cccagtcggt gaagcgatag aagtgcatgg ttgacggggc cggcatcgta
     1021 atttttgccg tttgttttgt attttgagaa acaaatccga attcatcgag cgtaatcggc
     1081 gccagccggc tcaccttgcc ctctgcgtac ggcgcggtga agtccgactc gtgcccgcgc
     1141 tcgtcgtgga agcggaacac cccgtccttc accaccaggc ccttggtgag gcggacgaac
     1201 ttttcccagt aggagatacg ccggaactcg ccgtcggtga cgacctgcag gccgcagtct
     1261 tcctgaagct tcaccgcatc gcggatcgcc tcgtcctgcg cggcgcggaa ttggccatcc
     1321 ggcatctcgc ccgaggcatg cttgcggaaa gcctcgcgca gctgcttcgg cctgagcagg
     1381 ctgccgatat gatcggcacg aaacggaggg gtcatcgccg ctggaattct atccggccgc
     1441 aagatcgagc cgccgtcacc gcaagctggc cctagtctga gagcagctct atgacgagct
     1501 cgcgcagcca gcggttgccc gggtcggctt cgaagcgctc gtgccagtgc accgcgacgt
     1561 cggcgggcgg gatcggcacc ggcggcggca gtgacttgaa cttgccgcga cgctcgaaca
     1621 cgcgcgccac tctcgccggc agcgtgagga tgagatcgct gcgctcgagc accatcggca
     1681 ccacggtgaa atgcggcacc cgcagcgcga tgcggcgcgc gagcccggca cgctcgagcg
     1741 cttcctcgat gacgcggtgc ccgccgcggt acgagacgag cgcgtgcgac gcttcgacga
     1801 acttcttttt cgtcaatgcc tggatcggat ggtcggcgcg catcaggcag acataaggat
     1861 cgcgaaacag gcgcctgcgg cgcaccggcg gcccgaggcc gggaagaaat cccaccgcaa
     1921 gatcgagcgc tccggccgcg agcgcgccgt cgatgtcctc cacgtcgagc gcgaccgcct
     1981 cgaagcgcac gcccggagcc tcgcgatgcg cgcgctcgac gagcggcggc aggaactcga
     2041 cctggccgag gtccgacatg taaaaacgga aggcgcgcgt cgaggtcgac gcatcgaatc
     2101 ccggaccatg cgcgagcgcc gactcgagga gcgcaagcgc ctggcgcacc ggctcggcga
     2161 gctcgcgggc aaacggcgtc gcgtccatgc ccgcgggcgt gcgcacgaac agcgcatcgc
     2221 cgaacagcgc gcgcagccgc gcgagcgcgt tcgacaccgc cggctgggtc agtccgaggc
     2281 gcaccgctgc cggggtcacg ccgcgcgcgg cgagcacggc gtcgaacacg cgcaacaggt
     2341 ttagatcgac gtcctggaca ttcacagcag tgattcataa catgaacgtc attgatttga
     2401 caaatataat cccaggcgcc taacctgtgc ggttccaaag gaggagtgca tgaaaaaact
     2461 gttgctcatc gccctggcag cgctggtgcc gtcgctcgcc ggggctcaag agacgacgct
     2521 gcgcgtggta agcgccttcg ccgagaacac gcagtacgtg aagaatctcg agcccttcat
     2581 gcagaagctc aacgccgagg gcaaggggag cctgcagctc aatttcatcg gcggcccgaa
     2641 agcgatgccg cccttcgagg tcggcaacgc ggtgcgcacc ggggtggtcg acgtcgccat
     2701 gaccacgggc gcgttctaca ccaacatcat gcccgaggcc gacgcgctca agctcacgca
     2761 gattcccgcc accgagctgc gcaagaacgg cggctacgag ctgatcaacc gcatctggaa
     2821 cgagaaggcc aacatgcagt acctcggccg ggtgatcgac ttcacgccgt tccatctcta
     2881 tctcaccaag aagatcgaca agcccgacct cacggggctg aagatccgca tcacgccggt
     2941 ctaccgcgag ttcttccagg cgctcggcgc gaccgtaatg cagaccgcgc ccggcgaggt
     3001 ctacaccgcg ctcgagcgcg gcgtggtcga cggctacggc tggccgatca acggcatctt
     3061 cgacctcaac tggcacgaga agaccaagtt ccgcgtcgat ccgggcttct acaacgccga
     3121 ggtgtcgctc gtcatgaacc ttgacaagtg gaagagcgtg acgcagccgc agcgcgatct
     3181 cctgatgcgc cacgtcatcg cgctcgaggc gcagaacgac tcgtggaaga aagtcaacgc
     3241 ggacgacatc aagcgccagg cgcaggcggg catccaggtc atcagtttcg acccggcgac
     3301 cgcgaagcag tactacaaca aggcctacga ggtgggctgg gcgagcgtca tcaaggcgag
     3361 cccgcagtac gggcccgaga tgagaaagct gttcagccgg tgaagcttta ctactcgctg
     3421 ctgaaaatac tcgccggcgc ggcagcgctt cttctcggcg cgatggcgtt gctcgtcacg
     3481 ctcgacgtgg tggcgcgcaa cgtcggtctg ggcaccatcg cgtgggtgaa cgaggtgtcg
     3541 gagtacagcc tgccggtcgc cacgctgctc gtcgcgccct ggctgcttca ccgcaacgag
     3601 cacgtgcgcc tcgacgtttt gcttgtgtta cttcccaggc aggttgcaaa aatcctggaa
     3661 cggtgcgcgg acctcatggg aatcgcgatc tgcgcggtgt tcgtctggta cggggccaag
     3721 ctgatcctcg actcggcgcg catcgattcg atggtggtga agacgctcgc cattcccgag
     3781 tggtggcagt acgcgctggt gccggtgtgc ttcttccttc tcgccgtcga gttcgcacgg
     3841 cggctgcgtg catgacatgg tggctgtcag cgatcgtcct gttcggagtg ttgacggctt
     3901 tcttcctgat ggggctggcg gcggcgttcg ctttcttcgg catcaacatc gtcggcgcct
     3961 ggttctatct cgagaaggaa gcggggctgg tgcagctgat ccgcaacggc atcgtctcga
     4021 tcaccagttt ctccctgacg ccgatcccct tcttcatcct catgggcgag gtgctgttcc
     4081 acaccggggt ggcgatgaag gcgatcgacg ccatcgaccg gctgatctgg cgcgtgccgg
     4141 gacgcctgtc ggtggtcgcg gtgctcgccg gcaccgtgtt ctcggcgatt tccggatcga
     4201 ccatcgccac caccgcgctg ctcggcagcc tgctgctgcc gcagatgctc gcgcggggct
     4261 atcaccccag catggcgatg gggccgatca tggcgatcgg cggcgtcgac atgctgatcc
     4321 cgccctcggc gctcaccgtg ctgctcggca gcctcgccgg catctcgatc tcgcagctgc
     4381 tgatcgccgg catcgtcccg ggcgtgctgc tctccgtcat gttcgtcgcc tggatcgtgc
     4441 tgcgcgcgat gctcgacccc tcgcttgcgc cagccttcga gcacgcggag gtctcgccct
     4501 gggagcggct gaagccgctg ctgctgtacg tcgtgccttt ggtgctgatc ttcgtcgtcg
     4561 tcgtggcagc gatggccgcg ggctgggcga ccccgacaga agcagccgcg atcggcgcgg
     4621 tggcgacggt ggcggtggcc ggcgtctacc gggccctcac gcttccgaac ctcatgcagg
     4681 ccctcatggg tacggccggc atttcgggaa tcatcctgtt catcatcatc ggcgccacca
     4741 cgttctcgca gatcctgacc ttctccggcg cgaccaacgg cctggtcgag gcgatcaagc
     4801 aagcctcctt tggtccttgg acggtgctcg tcgccatgat gctgatcctg ctggtgctcg
     4861 gctgtttcat cgaccaggtc agcatgatgc tcatctgcct gccgttcttc atgccgctgg
     4921 tcgagctcta caggttcgat ccgatctggt tcggcgtgct gttcctgatc tgcatgcagc
     4981 tcgggctgct gacgccgcct ttcggcatgc tgctcttcac catgaaagcg gtcgcgccga
     5041 aggaaatcga gatgcggcag gtgtggcacg cggtgacgcc gtatgtggtg atggggctcg
     5101 gcgcgctggt ggcggtgatc ctgtttccat ctctcgccac ctggctaccc aaggttcttt
     5161 ttaggtaact tatggaacgc tctttcaagc aggaagttgc aaagctgaag ctcggcgacg
     5221 gcgagacttt ccacggcgaa ggcatcctcg cagtgaccaa ggcgctgctg cagtcgggcg
     5281 tctcgtacgt cggcggctac cagggcgcgc cggtgtcgca cgtgatggac gtgctgaacg
     5341 acgcccgcga catcctcgac gagctcggcg tgcacgtcga agcgaatgcc aacgaggccg
     5401 gcgccgccgc gatgctcggc gcctcgatca actatccgat gcgcggcgcg gtggccttca
     5461 agtcgacggt gggcaccaat gtcgcgtccg acgcgctctc gaaccttgcc tcggcgggag
     5521 tgaaag
//
LOCUS       DASPXW010000218         3581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_443177, whole genome shotgun sequence.
ACCESSION   DASPXW010000218 DASPXW010000000
VERSION     DASPXW010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443177"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..790
                     /locus_tag="VEL04_05115"
     CDS             47..790
                     /locus_tag="VEL04_05115"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008342875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HYT47777.1"
                     /translation="MNTSPVRAPRFHLATPLRAGTTCVLPEEIAHHAVHVLRVRSGDE
                     ITLFDGRGGEYAARVVAVDRLRVTADVLEHRAVERESPLRVVLVQGVSAGERMDFTIR
                     KAVELGVAEIRPVLAAASVARPKGERAAARHTHWQKVAVAACEQCGRNRIPQIHPLTA
                     AADYREASGNLKILLAPRSELAFSEACRKVGTAITLAAGPEAGFNAAEEAAFLDCSFV
                     PARLGPRVLRTETAALAALAALNALRGDF"
     gene            832..2148
                     /gene="argA"
                     /locus_tag="VEL04_05120"
     CDS             832..2148
                     /gene="argA"
                     /locus_tag="VEL04_05120"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HYT47778.1"
                     /translation="MAALHPEAFVRWFRQVAPYVHAFRGRTFVVGFGGEMFAERSRFS
                     SFVHDVNLLAALGIRLVLVNGARPQIEAELKAKGLRSRYAQGLRITDEAALMAVKHAA
                     GVLRVEMEALLSQGLPSSPMAGAQIRASSGNFITARPAGVRRGTDFQFTGAVRKVDAA
                     AIARSLDAGEVVLVPHLGYSPTGEVFNLAWEDVAESVALSLRADKLLMYTDRLPLDRK
                     GEVLGELTAREAEALVKKGGLSSQTARAIEHAVRAVGAGVARAHLVTRRASSALLLEL
                     FTHTGVGTMITADAVEKLRPARIDDVRGILALIEPLEAEGTLVKRSRELLEAEIGNFL
                     VVEHDGVIVGCAALYPFPGDKSAEFACLAVAHDFRDAGYGERLLKSCEERARGLRIRR
                     LFALTTHAAHWFLEQGFRAADVDALPSRRQALYNWRRGSKVFLKRL"
     gene            2157..2432
                     /locus_tag="VEL04_05125"
     CDS             2157..2432
                     /locus_tag="VEL04_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HYT47779.1"
                     /translation="MARMVKCIKLGREAEGLDFPPYPGELGKRIWEKVSKEAWQQWLK
                     QQTMLVNENRLNLADPKARKYLMEQTQRHFFGEGADVASGYVPPEKQ"
     gene            complement(2503..3171)
                     /gene="rpiA"
                     /locus_tag="VEL04_05130"
     CDS             complement(2503..3171)
                     /gene="rpiA"
                     /locus_tag="VEL04_05130"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HYT47780.1"
                     /translation="MNQDQLKRNVAEAALKHIIEDAVVGVGSGSTVNFFIDALAALKG
                     RIEGAVAASEASAARLKQHGIRVFDLNSVDELPVYVDGADEVTEHLHMIKGGGGALTR
                     EKIVAAVAKRFVCICDASKLVPVLGRFPLPVEVIPMARSHVGRQLLRLGAHPELRQDF
                     RTDNGNLVIDCHGLTLLDPPKMEAELNNIAGVVTNGIFARRGADVLLLGEASGVRTLK
                     ARLA"
     gene            complement(3168..>3581)
                     /locus_tag="VEL04_05135"
     CDS             complement(3168..>3581)
                     /locus_tag="VEL04_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HYT47781.1"
                     /translation="AGMKSHFDARVIPSVAYTDPEIAWVGLTEDEAKKSGAKAQVAKF
                     PWSASGRAIANARDEGFTKLIFDGETHRILGGGIVGTGAGDLISELALAVEMGADATD
                     VGSTIHPHPTLSESVGMAAEAFEGVCTDLPPQRKK"
BASE COUNT          556 a         1210 c         1219 g          596 t
ORIGIN      
        1 acacccatga ggtcgaattc aggcgccttc atgcaaaaat cctagcatga acacgagtcc
       61 ggtccgcgcg ccgcgcttcc atctcgcgac gccgctgcgc gcgggaacga cctgcgtgct
      121 gccggaggaa atcgcgcacc acgcggtcca cgtgctgcgg gtacgcagcg gcgacgagat
      181 taccctattc gacggccgcg gcggcgaata tgcggcgcgc gtcgtcgcgg tcgaccggct
      241 gcgggtcacg gcggacgtgc tcgagcaccg cgcggtggag cgcgaatcgc cgctgcgcgt
      301 ggtgctggta caaggcgtgt cggcaggcga gcgcatggac ttcacgatcc gcaaggcggt
      361 cgagttgggc gtggccgaga tcaggccggt gctcgccgcc gcctcggtgg cgcgaccgaa
      421 gggcgagcgc gccgccgcgc gccacacgca ctggcagaag gtcgcggtcg cggcctgcga
      481 gcagtgcggg cgcaaccgga ttcctcaaat ccatccgttg acggccgcgg ccgattaccg
      541 tgaagcaagc ggcaatctga agatcctgct cgcgccgcgc agcgagcttg ccttttccga
      601 ggcatgccgg aaggtcggca ccgccatcac gctcgccgcc gggcccgagg cgggcttcaa
      661 cgcggccgag gaagccgcgt tcctcgattg cagcttcgtt ccggcgcgtc tcgggccgcg
      721 cgtgctgcgc accgagacgg cggcgctcgc cgcgctcgcg gccctcaacg cattgcgcgg
      781 ggatttctag cgagcgggtc aagacccctt agaatttcaa aaaaattcgc catggccgcc
      841 cttcatccgg aagctttcgt gcgctggttc cgccaggtgg cgccgtacgt gcacgccttt
      901 cgcggccgca ctttcgtggt cggcttcggc ggcgagatgt tcgccgagcg cagccgcttc
      961 agcagcttcg tgcacgatgt gaacctgctt gccgcgctcg gcatccggct ggtgctggtg
     1021 aacggcgcgc gcccgcagat cgaggcggag ctcaaggcca agggcctgcg ctcgcgctac
     1081 gcgcagggtc tgcgcattac cgatgaggcc gcgctcatgg cagtgaagca cgccgcaggc
     1141 gtgctgcgcg tcgagatgga agcgctgctc tcgcaggggc tgccgagctc gccgatggcc
     1201 ggggcgcaga tcagggcgtc gtcgggcaac ttcatcacgg cgcggccggc cggcgtgcgc
     1261 aggggcaccg atttccagtt caccggggcg gtgcgcaagg tcgatgcggc ggcgatcgcc
     1321 cgctcgctcg acgcgggcga ggtggtgctg gtgccgcacc tcggctactc gcccaccggt
     1381 gaggtgttca atctcgcctg ggaggacgtc gccgagagcg ttgcgctgtc gctgagggcc
     1441 gacaagctgc tgatgtacac cgaccggctg ccgctcgacc gcaagggcga agtgctgggc
     1501 gagctgaccg cgcgcgaggc cgaggcgctg gtgaagaaag gcggcctcag ctcgcagacc
     1561 gcgcgcgcca tcgagcacgc cgtgcgggca gtcggcgccg gagtcgcgcg cgcccacctc
     1621 gtgacgcggc gcgcctccag cgcgttgctg ctcgagcttt tcacccatac cggggtcggc
     1681 accatgatca ccgccgatgc ggtggagaag ctgcgcccgg cgcgcatcga cgacgtgcgc
     1741 ggcatcctgg cgctgatcga gccgctcgag gccgagggca ccctggtgaa gcgcagccgc
     1801 gagctgctcg aagccgagat cggcaatttc ctggtggtcg agcacgacgg cgtgatcgtc
     1861 ggctgcgccg cgctgtatcc ctttcccggc gacaagagcg cggaattcgc ctgcctcgcg
     1921 gtggcgcatg atttccgcga tgccggctac ggcgagcgcc tgctcaagag ctgcgaggag
     1981 cgcgcccggg ggctgcgcat caggcgcctg ttcgcgctga ccacgcacgc ggcgcactgg
     2041 tttctcgagc agggcttccg cgccgccgac gttgacgcgc tgccctcgcg ccgccaggcg
     2101 ctgtataact ggcgccgcgg ctcgaaggtg ttcctcaaga gactataatt cgcgccatgg
     2161 cgcggatggt gaagtgcatc aagctcggcc gcgaggccga ggggctcgat ttcccgccct
     2221 atccgggcga gctcggcaag cgcatatggg agaaggtctc gaaagaggcg tggcagcagt
     2281 ggctgaagca gcagaccatg ctggtcaacg agaatcgcct caacctcgcc gaccccaagg
     2341 cgcgcaaata tctcatggag cagacccagc gccatttctt cggcgaaggc gcggacgtcg
     2401 cctcgggcta cgtgccgccc gagaagcagt agctactcga ggatttcctt ctcgacctgc
     2461 tcgatcgcga tgtgccgcac gtcgcgcccc ttgaccggaa tgttacgcca gccgggcctt
     2521 cagcgttcgt acgcccgacg cttcaccgag caacagcaca tcggcgccgc ggcgcgcgaa
     2581 gatgccgttg gtcaccacgc cggcgatgtt gttgagctcg gcttccattt tcggcgggtc
     2641 gagcagcgtc aggccgtggc agtcgatgac caggttgccg ttgtcggtcc tgaagtcctg
     2701 ccgcagctcg ggatgggcgc cgagcctgag gagctgccgg ccgacgtggc tgcgcgccat
     2761 cgggatgact tccaccggca gcggaaacct gccgagcacc ggcaccagct tcgaagcgtc
     2821 gcagatgcaa acgaaccgct tcgccaccgc ggcgacgatc ttctcgcgcg tcagcgcgcc
     2881 gccgccgccc ttgatcatgt gaaggtgctc ggtcacctcg tcggcgccgt cgacgtacac
     2941 cggcagctcg tcgacgctgt tcaggtcgaa tacgcggatg ccgtgctgtt tcagtcttgc
     3001 cgcgctcgct tccgaggcgg ccaccgcgcc ctcgatgcgc cccttgaggg cggcgagcgc
     3061 atcgatgaag aagttgacgg tcgatcccga gccgacgccc accaccgcat cttcgatgat
     3121 gtgcttgagc gcggcttcgg caacgttcct ttttaactgg tcctgattca ttttttgcgt
     3181 tgcggcggca ggtcggtgca cacgccctcg aaggcctcgg ccgccatgcc gacggactcg
     3241 gagagcgtcg gatgcgggtg gatggtgctt ccgacatcgg tcgcgtccgc gcccatctcg
     3301 accgcgagcg ccagctcgct gatcaagtcg cccgccccgg tgccgacgat gccacctccc
     3361 agtatccgat gggtttcacc atcaaaaatc aactttgtga agccctcgtc gcgcgcgttg
     3421 gcgattgcgc gccccgatgc gctccacgga aacttcgcga cctgcgcctt ggcgccactc
     3481 tttttcgcct cgtcctcggt gaggccgacc catgcgatct cgggatcggt ataggcgacc
     3541 gaaggaatga cgcgcgcatc gaagtgcgac ttcatgcccg c
//
LOCUS       DASPXW010000219         3429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1076938, whole genome shotgun sequence.
ACCESSION   DASPXW010000219 DASPXW010000000
VERSION     DASPXW010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076938"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="VEL04_05140"
     CDS             <1..799
                     /locus_tag="VEL04_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HYT47782.1"
                     /translation="FAQSTPETVWGYYGLTPEQAKTGGLNPKMFNSFLDGSKPALEMT
                     AVCNATGLTPAPEGLAFPPGSVDEIPALMRPKAEGGVLDHKGQVEVVSSLRTDGTPIS
                     YDIRFGVFVVFEGESEYIRRCFMEYGVKTDPSGRYACMYKRWHLIGLEVGISVASVGL
                     RGEPTGCASGWRADAVAIAKRNLSKGEMLDGEGGYTVVGRLMPAADSLAQHCLPLGLA
                     HGWKLLRPVSAGQPVKWSDVAFDASSTAVRVRREMEQSFGERLRPAA"
     gene            complement(804..1766)
                     /locus_tag="VEL04_05145"
     CDS             complement(804..1766)
                     /locus_tag="VEL04_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYT47783.1"
                     /translation="MQFLRCLLSVLVGSMSAAALAQPYPGKAVRVMVGASPGGGTDII
                     ARMLADKFAERLKQPFIVENRPGASNTIAADVTAKAPPDGHTLLVATNTAQAIAPHLI
                     KLGFDPLKDVQPVGLIVVVPNVLVVGPSVPAKTLEELLAAVKSRPGEFRYATAGVGST
                     QHIAAEAFARATGTRLVHVPYKGSSQAHVDLIGGQVEMMFDTSSSAMGQIKGGRLRPL
                     AVTTPKRSAELPNVPTLAEAGVQGAEMSTWYGLFTTGGTPRPIVERLHAELQNVLRLP
                     DVQGRLTGLGGEPGALSVQQFAEMNRAEFERFGRLIREAGIKSD"
     gene            complement(1782..2714)
                     /locus_tag="VEL04_05150"
     CDS             complement(1782..2714)
                     /locus_tag="VEL04_05150"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HYT47784.1"
                     /translation="MRRARGRGCVPLREGSVQGRRLKTVAFIGLGRMGQPMAANLARE
                     GFALRSFDLNGSGSCRSIREAAKGAEALITMLPDGDAVREAVLDALPALKAGSVVIDM
                     SSSDPVGTRALGLALSRNNVQFVDAPVSGAVAGAKDGTLAIMAGGNASTIKRISSILK
                     TMGNRIFHVGPLGAGHAVKALNNYLGAAGTLAGFEALLVARAFGLQPRPMIEAFNAST
                     GRNYTTERKIPQQVLTGAFASGFRLALMAKDVGIAAGLARDLGVETPYLDHTLKLWRD
                     AQRKLPRGADHTEIYRYLERLGRPKKKPRVHRGK"
     gene            complement(2648..3034)
                     /locus_tag="VEL04_05155"
     CDS             complement(2648..3034)
                     /locus_tag="VEL04_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HYT47785.1"
                     /translation="MDKRNHKERYDAGLKTRREVLGAPWVDKAMAGVDEFNQEFTELL
                     NTYCWNDIWNRPGLPRKLRSVLNLGMLCALGKEHELKLHIRGALNNGLTRDEIKEVFL
                     QVAIYCGVPAAVAAFRCAKEVFKEGG"
     gene            3063..>3429
                     /locus_tag="VEL04_05160"
     CDS             3063..>3429
                     /locus_tag="VEL04_05160"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYT47786.1"
                     /translation="MDIKSLHHVAYRCKDAKQTADFYTKVLGLEYAMAVSEDHVPSTG
                     EEHPYMHIFFRMDDGSFVAFFELPESPAMGRDGNTPLWVQHLALRVPDEETLMRYKQR
                     IESHGVEVLGPVDHTICKSI"
BASE COUNT          547 a         1142 c         1116 g          624 t
ORIGIN      
        1 tttcgcgcaa tcgacgccgg agacggtgtg gggctactac ggcctcacgc ccgagcaggc
       61 gaagaccggc ggcctcaatc cgaagatgtt caactccttc ctcgacggct cgaagccggc
      121 gctcgagatg accgcggtgt gcaacgccac cggcctcacg cccgcgcccg aggggctcgc
      181 cttcccgccc ggctcggtcg acgagattcc cgcgctgatg cggccgaagg ccgagggcgg
      241 cgtgctggac cacaaggggc aggtagaagt ggtgtcttcc ttgaggaccg atggaacccc
      301 gatttcctac gacatccgtt ttggtgtctt tgtcgtgttc gagggcgaga gcgaatacat
      361 ccggcgctgc ttcatggaat acggcgtcaa gaccgaccct tccggccgct acgcctgcat
      421 gtacaagcgc tggcacctga tcgggctgga ggtcggcatc tcggtcgcct ctgtcggctt
      481 gcgcggcgag cccaccgggt gtgcgagcgg ctggcgtgcc gatgccgttg caattgcaaa
      541 aaggaatttg agcaaaggcg agatgctcga cggcgagggc ggctacaccg tggtcggaag
      601 gctgatgccg gcggccgatt cgctcgccca gcactgcctg ccgctcggtc tcgcccacgg
      661 ctggaagctg ctgcggccgg tgtcggccgg ccagccggtg aagtggagcg atgtcgcctt
      721 cgacgccagc tcgaccgcgg tgcgcgtgcg ccgcgagatg gagcaaagct tcggcgagcg
      781 cctgcgcccc gcagcttagc tgctcaatcg ctcttgatcc cggcttcccg gatcagtctt
      841 ccgaagcgct cgaactcggc gcggttcatc tcggcgaact gctgaacgct gagcgcgccg
      901 ggctcgccgc ccagaccggt gagccggcct tgcacgtccg gcagcctgag cacgttttgc
      961 agctcggcat gcagccgctc gacgatcggt cgcggcgtgc cgccggtggt gaacaggccg
     1021 taccaggtgc tcatctcggc gccttgcacg ccggcttcgg cgagcgtcgg cacgttcggc
     1081 agctcggccg agcgcttcgg cgtagtcacg gcgagcggcc gcagccttcc gcccttgatc
     1141 tgccccatcg ccgacgagct ggtatcgaac atcatttcca cctggccgcc gatgagatcg
     1201 acatgcgcct ggctgcttcc cttgtagggc acatgcacca gccgcgtccc ggtcgctctc
     1261 gcaaaagctt ccgcagcaat gtgctgggtg ctgccgacgc cggcggtggc gtagcggaat
     1321 tcgcccggcc tgcttttcac ggcggcgagc agctcttcca gtgtcttcgc ggggacgctt
     1381 ggacctacta ccagcacgtt cggcacgacc acgatcagcc cgaccggctg cacgtccttg
     1441 agtgggtcga agccgagctt gatgagatgg ggcgcgatcg cctgggcggt attcgtcgcg
     1501 acaagcagcg tatgaccgtc gggaggcgcc ttcgccgtca cgtcggcagc aatagtattg
     1561 gacgcccccg gccggttctc gacgatgaag ggctgcttca ggcgctcggc gaacttatcc
     1621 gcgagcatgc gggcgatgat gtcggtgccg ccgcccggcg atgcgccgac catgactctc
     1681 acggccttgc ccgggtaggg ctgcgccagc gcggcggcgc tcatcgaccc gaccaggacg
     1741 ctcagcaggc agcgaaggaa ttgcatcggc aggcctctcc tctattttcc tctgtgcacg
     1801 cgaggcttct ttttcggccg ccccagccgt tccaggtagc ggtagatctc ggtatggtcg
     1861 gcgccgcggg gaagcttgcg ttgtgcgtcc cgccacagct tcagggtgtg atccaggtaa
     1921 ggcgtttcca cgcccaggtc gcgcgccagg cctgcggcga taccgacgtc cttcgccatc
     1981 aatgccagtc tgaagccgga ggcgaacgcc ccggtcagca cctgctgcgg gatcttgcgc
     2041 tcggtggtgt agttgcggcc ggtggaggcg ttgaaggcct cgatcatagg cctgggctgc
     2101 aggccgaaag cgcgcgccac gagcagggcc tcgaagcctg cgagcgtgcc cgccgcgccg
     2161 aggtagttgt tgagcgcctt gaccgcgtgc ccggcgccga gcgggcctac atggaagatc
     2221 cggtttccca tcgtttttag tatcgaagaa atccttttta tcgtcgaggc gttgccgccc
     2281 gccatgatcg cgagagtgcc gtccttcgcc cccgctaccg cgccggagac cggcgcgtcg
     2341 acgaactgta cattgttcct ggaaagcgcc aaccccagtg cgcgcgtgcc caccggatcc
     2401 gacgagctca tgtcgatcac cacactgcca gccttgagcg ccggcagggc gtcgagcact
     2461 gcttcgcgca ccgcgtcgcc gtcgggcagc atggtgatca gcgcttccgc gccctttgcg
     2521 gcttcgcgga tcgagcggca gctgcccgaa ccgttgagat cgaacgagcg cagggcaaag
     2581 ccctcgcgcg cgaggttcgc cgccatgggc tgccccatac ggcccaggcc gataaaggcg
     2641 acggtcttca gccgccttcc ttgaacactt ccttcgcgca gcggaacgca gccacggccg
     2701 cgggcacgcc gcaatagatc gcgacctgca ggaagacctc cttgatctcg tcccgggtga
     2761 ggccgttgtt gagcgcgccg cggatatgca gcttcagctc gtgctccttg ccgagcgcgc
     2821 agagcatgcc gaggttgagc accgagcgca gcttgcgcgg cagcccgggc cggttccaga
     2881 tatcgttcca gcaataggtg ttgagcagct cggtgaattc ctggttgaac tcgtcgacgc
     2941 ccgccatcgc cttgtcgacc cacggcgcgc cgagcacctc gcggcgcgtc ttcagcccgg
     3001 cgtcgtagcg ttccttatga tttcgcttgt ccatcgctcc tccgtgagaa ataattgatt
     3061 atatggacat taaatctctg catcacgtcg cgtaccgctg caaggacgcg aaacagaccg
     3121 ccgatttcta caccaaggtt ctcggcctcg agtatgcgat ggccgtgtcc gaggaccacg
     3181 tgccgagcac cggcgaggag cacccctaca tgcacatctt cttccgcatg gacgacggca
     3241 gcttcgtcgc gttcttcgag ctgcccgagt cgccggcgat gggccgcgac ggcaacacgc
     3301 cgctgtgggt gcagcacctt gcgttgcgcg tgccggacga ggaaacgctg atgcgctaca
     3361 agcagcgcat cgagtcgcac ggtgtcgagg tgctcgggcc ggtcgaccac acgatctgca
     3421 agtcgatct
//
LOCUS       DASPXW010000220         5856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_822543, whole genome shotgun sequence.
ACCESSION   DASPXW010000220 DASPXW010000000
VERSION     DASPXW010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5856
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822543"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VEL04_05165"
     CDS             complement(<1..465)
                     /locus_tag="VEL04_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYT47787.1"
                     /translation="MRIRSVAVTAACALAFAHVANLQAADRIKIGFISTLSGPNAAIG
                     ADIRDAFQLAVKVNGGRLGGVPVEVLVGDDQFKPDVGRQLAEKYVKLDKVNFLTGIVF
                     SNIMLAAVPLAFENKTIYVSPNAAPSPFAGKQCNPYLFVASWPNDAYHEAAGQ"
     gene            502..1548
                     /locus_tag="VEL04_05170"
     CDS             502..1548
                     /locus_tag="VEL04_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47788.1"
                     /translation="MPFRARTPEAAPLAGAPDAAAVKRRALELGADLAGIASAATLNA
                     FPPDPRWPQTPERISPYVKSVIVLVQRIPAGAFRTKTNVPVQYMDMLVLRKMDKVAYR
                     LADELERLGHPSFVTAAQETDWSYKRASYGRLSTRHLGVEAGLGTLGLEVNILTPEFG
                     PRVYLTGILTELELEPSERITEQVCIGESCSRCLHSCPPNAVLHWGIDKRACASEAQE
                     FGFMTMLQFMERVIDAPALERSPMLKTVDLFGFWQGLLRVVGSFGDCPRCLAVCPVGN
                     DYHAHLADLQKVIPEKTPEKVSLQKQFQQARKSGADMPGLSQWNARWVGEEGYKGIVA
                     RQLQEFKKRQNENL"
     gene            1535..2473
                     /locus_tag="VEL04_05175"
     CDS             1535..2473
                     /locus_tag="VEL04_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47789.1"
                     /translation="MKIYKKPLTSHEIKAKALGLGADLVGIADGAALEVSHITELDGG
                     RAIVLAKRLNEGVARINRWNDRHKYYNDELALTHLEETSLELVYWLEDCGYPALIVPP
                     THVDPGEYQGNPREHMQTMLSLPHAAVEAGLGTLGLNLQLLTPQYGPRVILTAVLCSV
                     EVECDRPMTEALCRGPACGRCLKTCPADAVGHWERDWPACDTCRSPHGFAQLTAHLEK
                     IIHESDAAARKQLLRSEDSFNLWQSILRGAGVVTGCRRCADVCPVGEDYAMLADALEE
                     IPENTPEKERRLAAMIDARPESYEKQKRFIGFLKQI"
     gene            2518..3804
                     /locus_tag="VEL04_05180"
     CDS             2518..3804
                     /locus_tag="VEL04_05180"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYT47790.1"
                     /translation="MPLPLPQSRKEIRRTQSERKRIAVENALRSPFWKKRIAKNISLE
                     KLDDPEVWRSIPILDKDTLRALTDQQFYAEFCIPPADGIAEYWRSGGVTGQPLFYPRS
                     FRDMQFGLEAFARTYDCAGAGRSDRAHVSFPMGIHPVGQVFARCAASRGITVNWAGSG
                     ASTPSALQLELIQRLRPTVWLGMSSYALHLANLAEVRGLDLSSVKKIMTSAEPVSQAK
                     RDKIERSWGARLTDTFGMTEAGMMGAEDQPGLGFRIWTDLYFVEVLDPKSLEPAKEGE
                     VGTLVVTALCSNNVTPFLRWSSGDLVTYSEADDGAGPFSVFPRIKHAHRTTGFFKVRG
                     VNLGHQDLEDFMFRHQEIGDFRAEAVNEGGLDALRLSIEVRKGIDAGALSSALSLAMK
                     EKFELAPEVVVLETGTLAREFESNIKAARFVDRRGG"
     gene            3814..4821
                     /locus_tag="VEL04_05185"
     CDS             3814..4821
                     /locus_tag="VEL04_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYT47791.1"
                     /translation="MKRENVIGRANVEDTAELEAYYRELEKRELGALWNVANEIEPWY
                     PQPKSVPVHWKWRDIEPLVRKAAELVTAERAARRVVMLVNPGRREWSAAAGLLYTGVQ
                     VMNPREFTSAHRHQASALRFVMEGKGAFTVVDGERMELGARDFVLTPNGTWHDHGVDE
                     GGTQCIWQDGLDIPLMNSLDANFYEVHPQTVQTPAPLSREGWKKPYSPVFLYKWDESY
                     QALKKDGAVYEYKNPLTGGAVMPTMGARLELIKKGASKKPARHTGSVIYQVASGRGWS
                     EVGGQRFEWEEKDIFCLPSWAVHSHANASSTEDAVLFSFNDFPAMRALALYREEAVAE
                     R"
     gene            complement(4818..5702)
                     /locus_tag="VEL04_05190"
     CDS             complement(4818..5702)
                     /locus_tag="VEL04_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYT47792.1"
                     /translation="MDSRRRALLIAMAAGPVFAEEKRLISKPIPSSGERLPAIGVGTW
                     QTFDVGGNAAARSPLREVLRLLNGNVVDSSPMYGSSESVAGDLIAELGVRDRLFIATK
                     VYTSGRDAGIRQMEDSFRRLRVERMDLMQVHNLVDVATHTGTLKNWKRKGRVRYIGIT
                     HYTSSAYADVERLLKTGDYDFLQINYSLGEREAERRLLPLALEKKVAVIINRPFAEGN
                     LFRRTKGKSLPPWADELGVASWAQYFLKWIVSHPAVTCAIPGTGKPEHMRDNLAAGSG
                     ALPNDGERRRMASYFDSL"
     gene            complement(5630..>5856)
                     /locus_tag="VEL04_05195"
     CDS             complement(5630..>5856)
                     /locus_tag="VEL04_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47793.1"
                     /translation="AKNFIDTVVYRGGDALSARVFTGFAGIAFVAAGVAVLWTALAFY
                     LGARMKSWTRAGAPSSSPWPPDPSLPKKRD"
BASE COUNT         1055 a         1908 c         1930 g          963 t
ORIGIN      
        1 ctggccggcc gcctcgtggt aggcgtcgtt cggccaggag gcaacgaaca ggtacgggtt
       61 gcactgcttg ccggcgaacg gcgagggcgc ggcgttcggg ctgacgtaga tcgtcttgtt
      121 ctcgaacgcc agcggcaccg ccgcgagcat gatgttggag aacacgatgc cggtgaggaa
      181 gttgaccttg tcgagcttga cgtacttctc ggcgagctgg cgcccgacat cgggcttgaa
      241 ctgatcgtcg cccaccagca cctcgaccgg aacgccgccg agcctgccgc cgttcacctt
      301 caccgcgagc tggaacgcgt cgcggatgtc ggcgccgatg gcggcgttgg ggcccgagag
      361 cgtgctgatg aagccgattt ttatccggtc cgccgcctgt aaattggcta catgcgcgaa
      421 cgcgagcgcg cacgcggcgg tcacggcgac cgatctgatt cgcatggctt ttccctcccc
      481 taatgattct acaatacgag catgcccttc cgagcgcgca ctccggaggc cgcaccgctc
      541 gccggtgctc ccgatgccgc ggcggtgaag cgccgcgcgc tcgagctcgg ggcggacctg
      601 gcgggcatcg cgagcgcggc gacgctcaat gcgttcccgc cggacccgcg ctggccgcag
      661 acccccgagc gcatctcgcc ttacgtgaag agcgtcatcg tgctggtgca gcgcattccg
      721 gcgggcgcgt tccgcaccaa gaccaacgtc ccggtgcagt acatggacat gctggtgctg
      781 cgcaagatgg acaaggtggc gtaccggctc gccgatgagc tcgagcgcct ggggcacccg
      841 agcttcgtca ccgcggcgca ggaaaccgac tggagctaca agcgcgcttc ttacgggcgg
      901 ctgtcgacgc gccatctcgg cgtcgaggcg gggctcggca cgctcgggct cgaggtgaat
      961 atcctcacgc ccgagttcgg cccgcgcgtc tacctcacgg gaattctcac cgagctcgag
     1021 ctcgagccca gcgagcgcat caccgagcag gtctgcatcg gcgagtcgtg cagccgctgc
     1081 ctgcattcct gcccgccgaa cgcggtgctg cactggggca tcgacaagcg cgcctgcgcc
     1141 tccgaggcgc aggagttcgg tttcatgacc atgctgcaat tcatggagcg tgtcatcgat
     1201 gccccggcgc tcgagcgctc gccgatgctg aagacggtcg atctgttcgg cttctggcag
     1261 ggattgctgc gcgtggtcgg ctcgttcggc gattgcccgc gctgcctcgc ggtgtgcccg
     1321 gtgggcaacg actaccacgc gcatctcgcc gacctgcaga aggtgatccc ggagaagacg
     1381 cccgaaaaag tttccctgca aaagcaattt cagcaggcgc gcaagtcggg cgccgacatg
     1441 ccgggcctgt cgcaatggaa cgcgcgctgg gtaggagagg agggctataa gggcatcgtc
     1501 gcccggcagc tccaggaatt caagaaacgc cagaatgaaa atctataaga agcctctgac
     1561 ttcgcacgag ataaaggcca aggcgcttgg gctcggcgcc gacctcgtcg gcatcgccga
     1621 tggcgccgcg ctcgaggtct cgcacatcac cgagctcgac ggcggccgcg ccatcgtgct
     1681 cgccaagcgc ctgaacgaag gcgtcgcccg catcaaccgc tggaacgacc gccacaagta
     1741 ctacaacgac gagctcgcgc tgacgcatct cgaggagacc tcgctcgagc tggtgtactg
     1801 gctcgaggac tgcggctacc cggcgctcat cgtgccgccg acgcacgtcg atcccggcga
     1861 gtaccagggc aacccgcgcg agcacatgca gaccatgctg tcgctgccgc acgccgccgt
     1921 ggaggcgggc ctcggcacgc tcggcctgaa cctgcagctg ctgaccccgc aatacggccc
     1981 gcgcgtgatc ctcaccgcgg tgctgtgctc ggtcgaggtc gaatgcgacc ggccgatgac
     2041 cgaggcgctg tgccgcggcc ccgcctgcgg gcgctgcctc aagacctgcc ccgccgacgc
     2101 ggtcgggcac tgggagcgcg actggccggc gtgcgacacc tgccgctcgc cgcacgggtt
     2161 tgcacagctc accgcgcatc tggaaaaaat aattcatgaa agcgacgcgg ccgcccgcaa
     2221 gcagctgctg cgctccgagg acagcttcaa cctgtggcag agcatcctgc gcggggcggg
     2281 cgtcgtcacc ggctgccgcc gctgcgccga cgtttgcccg gttggcgagg actacgcgat
     2341 gcttgccgac gcgctggagg agatcccgga gaacacgccg gagaaggaac gccgcctcgc
     2401 cgcgatgatc gatgcccggc cggagtccta cgaaaagcag aaacgcttca tcggcttcct
     2461 gaagcagatc tgagcaaaaa tggggacaga ccggacagac cccatttttc tcatttgatg
     2521 ccgttaccgc tcccccaatc ccgcaaagag atccggcgca cccagagcga gcgcaagcgc
     2581 atcgcggtcg agaacgcgtt gcgctccccg ttctggaaaa aaagaattgc aaaaaacata
     2641 agtcttgaaa aactcgatga cccggaggtg tggcgcagca tcccgatcct cgacaaggac
     2701 accctgcgcg cgctcaccga ccagcagttc tatgcggagt tctgcatccc tcccgccgac
     2761 ggcatcgccg agtactggcg ctcgggcggg gtcaccgggc agccgctgtt ctacccgcgc
     2821 agcttccgcg acatgcaatt cggcctcgag gccttcgcgc gcacctacga ctgcgcgggc
     2881 gcggggcgca gcgaccgggc gcacgtctcc ttcccgatgg gcatccatcc cgtgggccag
     2941 gtcttcgctc gctgtgccgc ttcgcgcggc atcacggtga actgggcggg ttcgggtgcg
     3001 tccacgccct cggcgttgca gctcgagctg atccagcgcc tgaggcccac cgtctggctc
     3061 ggcatgtcga gctacgcgct gcacctcgcg aacctggcgg aagtccgcgg tcttgacctg
     3121 agctcggtaa aaaaaatcat gacctcggcc gagccggtat cgcaggcgaa gcgcgacaag
     3181 atcgagcgca gctggggcgc gcgcctcacc gataccttcg gcatgaccga ggcgggcatg
     3241 atgggcgcgg aggaccagcc cgggctcggg ttccgcatct ggaccgacct gtacttcgtc
     3301 gaggtgctcg atccgaagtc cctcgagccg gcgaaggagg gcgaggtcgg cacgctggtg
     3361 gtgacggcgc tgtgcagcaa caacgtcacg ccgttcctgc gctggagttc cggggacctc
     3421 gtgacctaca gcgaggccga cgatggggcc gggcctttct cggtgttccc gcgcatcaag
     3481 catgcccacc gcaccaccgg cttcttcaag gtgcgcggcg tcaacctcgg ccaccaggac
     3541 ctcgaggact tcatgttccg tcaccaggag atcggcgact tccgcgccga ggcggtgaac
     3601 gaaggcgggc tggacgcgct gcgcctttcc atagaggtca ggaaaggcat cgacgccggc
     3661 gcgctttcca gcgccctgag cctggcaatg aaggagaaat tcgagttggc gcctgaagtg
     3721 gtggtcctgg agaccggcac cctggcgcgc gaattcgagt cgaacatcaa ggccgcgagg
     3781 ttcgtcgacc gccgcggggg ttaaactagc ctcatgaagc gcgagaacgt catcggccgg
     3841 gcgaacgtcg aggacactgc ggagctcgag gcctactacc gcgagctgga gaagcgcgag
     3901 ctgggcgcgc tttggaacgt cgccaacgaa atcgagccct ggtatccgca gcccaaatcg
     3961 gtgccggtac actggaaatg gcgcgacatc gagccgctgg tgcgcaaggc tgctgaactg
     4021 gtcaccgccg aaagagccgc gcgccgcgta gtgatgctgg tcaacccggg gcgtagggaa
     4081 tggagcgcgg ccgccggcct gctttacacc ggcgtgcagg tcatgaaccc gcgcgaattc
     4141 acctcggcgc accgccacca ggcctccgcc ctgcgctttg tgatggaggg caagggcgcg
     4201 tttaccgtgg tggacggcga gcgcatggaa ctgggtgcgc gcgatttcgt gctgacaccg
     4261 aacggcacct ggcacgacca tggcgtggat gaaggcggca cgcagtgcat ctggcaggac
     4321 ggcctcgaca tcccgctcat gaattcgctg gatgcgaatt tctacgaagt gcatccgcag
     4381 acagtccaga cgccggcgcc gctgtcgcgc gaaggctgga agaaacccta ttccccggtc
     4441 ttcctctaca aatgggatga gagctaccag gcgcttaaga aggacggcgc ggtgtacgag
     4501 tacaagaatc ccctgaccgg cggggcggtc atgcccacca tgggtgcgag actggaactc
     4561 ataaaaaaag gcgcatcaaa gaagccggcg cggcacacag gcagcgtcat ctaccaggtt
     4621 gcgtcagggc gcggctggtc ggaagttggt ggccagcgat tcgaatggga ggagaaggac
     4681 atcttctgcc tgccgtcatg ggccgtgcac tcgcacgcca atgcctcgtc caccgaggac
     4741 gcggtgctgt tctcgttcaa cgactttccg gcgatgcgcg cgctcgcgct gtatcgcgaa
     4801 gaggcagtcg ccgagcgcta gagggaatcg aagtagctcg ccatgcggcg gcgctcgccg
     4861 tcgttcggca gcgctccgga gccggcggcg aggttgtccc gcatgtgctc cggcttgccg
     4921 gtgcctggaa tggcgcaggt caccgccggg tgcgacacga tccatttcag gaagtactgc
     4981 gcccagctcg ccacgccgag ctcatctgcc cacggtggca gcgattttcc ttttgttctt
     5041 ctaaaaagat taccttccgc gaagggccgg ttgataatca ccgcaacttt cttctcaagg
     5101 gcgagaggca gaaggcgccg ctcggcttcc cgctccccca gcgagtagtt gatctgcagg
     5161 aaatcgtaat cgccggtctt caacaagcgc tcgacgtcgg cgtaggctga tgaggtgtag
     5221 tgggtgatgc cgatatagcg cacccgcccc ttccgtttcc agtttttcaa ggtcccggta
     5281 tgcgtggcga catcgacaag gttgtggacc tgcatcagat ccatgcgctc gacgcgcagg
     5341 cgcctgaacg agtcctccat ctggcggatg cctgcgtcgc ggccgctggt gtagaccttg
     5401 gtggcgatga agagcctgtc gcgcacgccg agctcggcga tcaggtcgcc ggcgaccgac
     5461 tcggaagagc cgtacatcgg cgaggagtcc accacattgc cattcaggag cctcagcacc
     5521 tcccgcaggg gcgagcgtgc ggccgcgttg ccgccgacgt cgaaggtctg ccaggtgccg
     5581 acgccgatgg cgggcagtcg ctcgccgctc gacggtatcg gcttcgagat cagtctcttt
     5641 tcttcggcaa agacgggtcc ggcggccatg gcgatgagga gggcgcgccg gcgcgagtcc
     5701 atgatttcat tctagcgccg aggtagaacg ccagcgccgt ccacagcacg gcgacgccgg
     5761 cggcgacgaa cgcgatcccg gcgaagcccg tgaagacgcg cgcggaaagc gcgtcgccgc
     5821 cgcgatacac cacggtgtcg atgaagtttt tcgcct
//
LOCUS       DASPXW010000221         3023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_571606, whole genome shotgun sequence.
ACCESSION   DASPXW010000221 DASPXW010000000
VERSION     DASPXW010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571606"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VEL04_05200"
     CDS             <1..373
                     /locus_tag="VEL04_05200"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HYT47794.1"
                     /translation="AGLNPHGGESGYLGTEDRDVIAPAVAEAAAAGIAASGPIPADTL
                     FVPERLRHADCVLAMYHDQGLPVLKYASFGRGVNVTLGLPFVRTSVDHGTALDLAGTG
                     RAEPGSLIEAVRLAIDLASAA"
     gene            351..1091
                     /gene="rsmA"
                     /locus_tag="VEL04_05205"
     CDS             351..1091
                     /gene="rsmA"
                     /locus_tag="VEL04_05205"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HYT47795.1"
                     /translation="MISHRRRKRFGQHFLHDPRVLARIVEAIGPSRDDFLVEIGPGEG
                     ALTRPLLERAGKLEAIELDRDLAAGLAAQFPPYQLTVHCADALEFDFGRFPAGLRVVG
                     NLPYNISTPLLFHLARHAQRMRDLHFMLQLEVVERMVAAPSSAAYGRLSVALQARFRM
                     KKLFKVSKGAFRPPPKVESAVVRLEPLPKILQINEDLLRRAFSARRKTLKNALPEIDF
                     AALGIDPRLRPENLSPEDYARLSMNSIL"
     gene            complement(1058..1444)
                     /gene="gloA"
                     /locus_tag="VEL04_05210"
     CDS             complement(1058..1444)
                     /gene="gloA"
                     /locus_tag="VEL04_05210"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HYT47796.1"
                     /translation="MRILHTMLRVGDLQRSVKFYTDVLGMKLLRTTDRPEQKYSLAFV
                     GYDGEDRSAVLELTYNYGVERYDLGSAFGHVAIEVPDVKRACEGVKRHGGKVTREPGP
                     VKGGTSVIAFVEDPDGYKIEFIERRA"
     gene            complement(1472..2176)
                     /locus_tag="VEL04_05215"
     CDS             complement(1472..2176)
                     /locus_tag="VEL04_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HYT47797.1"
                     /translation="MSLARSVLFAAALTVITPPYTLVALATFALPRMARYRIISGWSR
                     LVLFLAKTILGIDWRVEGRENLPSRPSVILAKHQSAWETLAFQHIFPPQVHVLKRELL
                     WIPFFGWGLALMSPIAINRSRGAAALRSVARRGRERLEQGFWVVVFPEGTRVAPGESR
                     EYQVGGAWLAAASGAPVVPVAHNAGLYWPRNAFLKRSGTVTVRIGPAIDSANRDPKEI
                     NAMARAWIEGQQKALV"
     gene            complement(2173..2706)
                     /gene="gmhB"
                     /locus_tag="VEL04_05220"
     CDS             complement(2173..2706)
                     /gene="gmhB"
                     /locus_tag="VEL04_05220"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HYT47798.1"
                     /translation="MKLVILDRDGTINHDSDNYIKSPDEWRPIEGSLDAIARLTQAGY
                     RIAVATNQSGIARKLFDTRTLFAIHDKLQRAVAQAGGRIDAFFFCPHAADSSCECRKP
                     KPGMLLEIARRFNVPLEEVYVVGDARRDVEAAAAAAARPVLVLTGKGRQTRAEGELPR
                     GTEVFADLAAFAERIAP"
     gene            complement(2703..>3023)
                     /locus_tag="VEL04_05225"
     CDS             complement(2703..>3023)
                     /locus_tag="VEL04_05225"
                     /inference="COORDINATES: protein motif:HMM:NF017555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HYT47799.1"
                     /translation="NILRQAEKKGASSVVDADISALKEPAEISLFHALQAASSSAKPL
                     FESGDFAGYLKTFASLKNPVDSFFDAVMVMDDDQRLRENRLALLTDMRKAMNRFADIS
                     KLAA"
BASE COUNT          497 a         1009 c          990 g          527 t
ORIGIN      
        1 cgcggggctg aatccgcatg gcggcgagag tggctatctc ggcaccgaag accgggacgt
       61 catcgcgccc gccgtcgcgg aggctgcggc cgcaggcatc gccgcgagcg ggccgatccc
      121 cgcggacaca ctgttcgttc ccgagcggct gcggcacgcg gactgcgtgc tcgccatgta
      181 ccacgaccag ggccttccgg tcctgaagta cgcatcgttc ggccggggcg tgaacgtgac
      241 cctcggtctg ccgttcgtgc gcacctcggt cgaccacggc acggcgctcg atctcgccgg
      301 caccgggcgc gccgaaccgg gaagcctcat cgaggcggtc aggcttgcca ttgatctcgc
      361 atcggcggcg taaaagattc ggtcagcact tccttcacga cccgcgggtc ttggcgcgca
      421 tcgtggaggc catcggtcct tcccgggatg actttctggt cgaaatcggt ccgggcgaag
      481 gcgcactcac ccggccgctg ctcgagcgcg ccggcaagct cgaggcgatc gagctcgacc
      541 gcgatctcgc ggcgggcctc gcggcgcagt ttccgcctta ccagctcacg gtgcactgcg
      601 ccgacgcgct cgagttcgac ttcgggcgct ttccggccgg cctgcgcgtg gttgggaatt
      661 tgccctacaa catctcgacg ccgctgctgt ttcacctcgc ccgccacgcg cagcgcatgc
      721 gcgacctgca tttcatgctg caactcgagg tggtcgagcg catggtcgcg gcgccctcga
      781 gcgcggctta cgggcggctg tcggtcgccc tgcaggcgcg cttccgcatg aaaaagctct
      841 tcaaggtctc gaagggcgcg ttccgtccgc cgccgaaagt ggaatcggcg gtcgtgcgcc
      901 tcgagccatt gccgaaaatt ctgcagatca acgaggattt gctgcggcgc gcgttctccg
      961 cgcgccgcaa gacgctgaag aacgccctgc cggagatcga tttcgcggcg ctcggcatcg
     1021 acccgaggct caggccggag aacctctcgc ccgaggacta cgcgcgcctt tcgatgaact
     1081 cgatcttgta gccgtcgggg tcctcgacga aggcgatgac gctggtgccg cccttcaccg
     1141 ggccgggttc gcgcgtcacc ttgccgccgt ggcgcttgac tccctcgcaa gcccttttca
     1201 cgtcgggcac ttcgatcgca acgtgaccga aggccgatcc caggtcatag cgctcgacgc
     1261 cgtagttgta ggtgagctcg agcacggcgc tcctgtcctc gccgtcgtag ccgacgaaag
     1321 caagcgagta tttctgctcc ggacgatcgg tggtgcgcag gagtttcatc ccgagcacgt
     1381 cggtatagaa cttgacggag cgctgcaggt cgccgacacg cagcatggta tgcaggattc
     1441 tcatgctatc tgtacctccc ggttctcaga atcagaccaa tgccttctgt tgcccttcga
     1501 tccatgcccg agccattgcg ttgatctcct tcgggtcgcg gttcgcggag tcgatcgccg
     1561 gaccgatccg cacggtgacg gtgccggagc gtttcaggaa ggcgttgcgc ggccagtaca
     1621 ggcccgcgtt atgcgcaacc ggcaccaccg gcgcgccgct cgcggcggcg agccaggcgc
     1681 ccccgacctg gtattcacgg ctctcgcccg gcgcgacgcg cgtgccttcg ggaaacacca
     1741 ccacccagaa tccctgttcg aggcgctcgc ggccgcggcg cgcgacgctg cgcagcgccg
     1801 cggcgccgcg cgagcggttg atggctatgg ggctcatcag cgcgaggccc cagccgaaga
     1861 acgggatcca cagaagctcg cgtttcagca cgtgcacctg tggcggaaag atgtgctgga
     1921 acgccagcgt ctcccaggca gactgatgtt tggcgaggat cactgagggc cgcgagggca
     1981 ggttctcgcg tccctcgacc cgccagtcga tacccaggat cgtcttcgct aaaaacagca
     2041 ccagccgcga ccagcccgaa atgatccggt agcgcgccat gcgcggcagc gcaaaggtgg
     2101 caagagcgac cagggtgtaa ggcggggtga tgacggtgag cgcagccgcg aacagcaccg
     2161 accgcgcgag gctcatggag ctatacgctc ggcgaacgcc gccaggtccg caaacacctc
     2221 ggtgccgcgg gggagctcgc cctcggcgcg cgtctggcgt cccttgcccg tcagcaccag
     2281 caccggtcgg gcggcggctg cggccgccgc ctcgacgtcg cgccgcgcat cgcccacgac
     2341 gtacacctcc tcgagcggca cgttgaagcg ccgcgcgatt tcgagcagca tgccgggctt
     2401 cggcttgcgg cactcgcacg aagagtcggc agcgtgaggg cagaagaaaa aagcgtcgat
     2461 ccgcccgccc gcctgcgcca ctgcgcgctg cagcttgtca tggatggcga acagcgtgcg
     2521 ggtgtcgaac agcttgcgcg cgatgcccga ctggttggtc gccaccgcga tgcggtagcc
     2581 cgcctgcgtg agccgggcga tcgcatcgag acttccctcg atggggcgcc attcgtcggg
     2641 cgacttgatg tagttgtcgc tgtcatggtt gatggtgccg tcgcgatcca ggatgacgag
     2701 cttcatgcgg cgagcttgga gatatcggcg aaccggttca tcgccttgcg catgtcggtg
     2761 agcagcgcaa gccggttctc gcgaagcctc tggtcgtcgt ccatcaccat gaccgcgtcg
     2821 aagaatgaat ctacgggatt cttcaacgag gcaaaggtct tcagatatcc agcgaaatcc
     2881 cccgactcga acagcggctt cgcggagctg gacgccgctt gcaaggcatg gaagagcgag
     2941 atttcggcgg gttccttgag agccgaaatg tcggcatcta caacagatga cgcgcccttc
     3001 ttttctgcct gtcgcagtat gtt
//
LOCUS       DASPXW010000222         2746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_192954, whole genome shotgun sequence.
ACCESSION   DASPXW010000222 DASPXW010000000
VERSION     DASPXW010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192954"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="VEL04_05230"
     CDS             <1..685
                     /locus_tag="VEL04_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYT47800.1"
                     /translation="YAYWMFQAFARENGRDLMNRDGNQTNFAHPDVIGALQYWRDLGA
                     KHKVMPEGTVEWGTLRQNFTEGKTAIMWHTTGNLTAVKDTAKFPFGVAMLPASRQRGS
                     PTGGGNWYLFKKSTPAERKSALEFIKWVTAPERTAEWSIATGYVGTRPDAYETPKLKE
                     YVSGFPAAAVARDQLKFATAELSTFQTGRVRKALDDAIQAALTGGKPPADALKAAQQE
                     ADRLLKPYR"
     gene            842..1705
                     /locus_tag="VEL04_05235"
     CDS             842..1705
                     /locus_tag="VEL04_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HYT47801.1"
                     /translation="MKHVYGWLLLAPAATLLVGFTHYPAAATLVESLFSTGTAARPSR
                     FAGLANYAALGDDPIFWQVLGNNFWFALGTIPTSIAAALLMALWVNDKLPGRALVRMS
                     YFTPTILPMIAVANLWLYFYTPQIGLLDKVTALFGAGSHNWLGDPHTVMGCLIAMTIW
                     KEAGFFMIFYLAALQSLPPELEEAGRIEGASRWYFFRRVTFPLLMPTTLFVLVNATIN
                     SFKLVDHLFILTKGGPDNASNLLLYYIYQVAFSFFDTAYASTLTVVLLFLLGAFAILQ
                     FQLIEKRVHYR"
     gene            1702..>2746
                     /locus_tag="VEL04_05240"
     CDS             1702..>2746
                     /locus_tag="VEL04_05240"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HYT47802.1"
                     /translation="MRRIRDLPGPPGLPLLGNLLQIDRTRLHLIAEAWCRTYGDYFRF
                     RIGSREYLVIADPEAIAAVLRDRPDGFQRTAGLNAAAREMGFGGVFSANGEQWRRQRP
                     MVMASFDPAHIKSYFPALVRVTRRFAGRWQRAAAAGGTIDLQADLMRYTVDVTAGLAF
                     GADINTLESDRDVIQSHLDKVLPALFARLMAPFVLWRWIGDLALGRHLAALHRAVDGF
                     IAAARQRMDAEPRLRARPTNLIEAMIAARDTEASGLDDADVAGNVLTMLLAGEDTTAN
                     TLAWMIYLLERNPAAMQRARDEVRAVLGAEPLPARYEQLSRLPFVEACAHETMRLKPV
                     APIIIKQAVHDTVV"
BASE COUNT          447 a          959 c          901 g          439 t
ORIGIN      
        1 ctacgcgtac tggatgttcc aggcgttcgc gcgcgagaac ggccgggatc tgatgaaccg
       61 ggacggcaac cagaccaatt tcgcgcatcc cgacgtcatc ggcgcgctgc agtactggag
      121 agatctgggg gcaaaacaca aggtgatgcc cgaaggcacc gtcgagtggg gcacgctgcg
      181 ccagaacttc accgagggca agaccgcgat catgtggcac accacgggca atctcaccgc
      241 ggtgaaggac acggccaagt tccccttcgg cgtcgccatg ctgcccgcct ccaggcagcg
      301 cggctcgccc accggcggcg gcaactggta cctgttcaag aagagcacgc cggccgagcg
      361 gaagagcgcg ctcgagttca tcaagtgggt caccgcgccg gagcgcaccg ccgagtggag
      421 catcgccacc ggctacgtcg gcacgcgccc ggatgcctac gagacgccga agctgaagga
      481 gtacgtgagc ggtttcccgg ccgcggcggt cgcgcgcgac cagctcaagt tcgccaccgc
      541 cgagctctcc accttccaga ccggccgcgt gcgaaaggcg ctcgacgacg cgatccaggc
      601 ggcgcttacc ggcggtaagc cgccggccga cgcgctcaag gcggcacagc aggaggcgga
      661 tcggcttctg aagccttacc gctgaaacag aacggacaac gcctgcgctt cgccgccccc
      721 ctgacaaggg ggaagcgagc ggctcttgcg agcggggggt tggttttttg cgaatgaaaa
      781 ccgaaacccc cctggcccgc cagaggcgag ccatcccccc ttgtcagggg ggcaaagaac
      841 catgaagcac gtgtacggct ggctgctgtt ggcgccggcc gcgacgctgc tcgtcggctt
      901 cacgcactac ccggcggcgg cgacgctcgt cgagagcctg ttctccaccg gcacggcggc
      961 gcgcccctcg cgcttcgccg gcctcgccaa ctatgccgcg ctcggcgacg acccgatctt
     1021 ctggcaggtg ctcggcaaca acttctggtt cgcgctcggc accatcccga cctcgatcgc
     1081 ggctgccctg ctgatggcgc tctgggtgaa tgacaaattg ccggggcgcg cgctggtgcg
     1141 catgagctac ttcacgccga cgatcctgcc gatgatcgcg gtggcgaacc tgtggctcta
     1201 tttctacacg ccgcagatcg gcctcctcga caaggtcacg gcgctgttcg gcgccggcag
     1261 ccacaactgg ctcggcgatc cgcacacggt gatgggctgc ctgatcgcca tgaccatctg
     1321 gaaggaagcc ggcttcttca tgatcttcta cctcgcggcg ctgcagtcgc tgccgcccga
     1381 gctcgaggag gcgggcagga tcgagggcgc ctcgcgctgg tacttcttcc gccgcgtcac
     1441 cttcccgctg ctcatgccga ccacgctctt cgtgctggtg aacgcgacca tcaactcgtt
     1501 caagctggtc gatcacctct tcatcctgac caagggcggc ccggacaacg cctcgaacct
     1561 gctcctctac tacatctacc aggtggcgtt cagcttcttc gacaccgcgt acgcctcgac
     1621 gctgacggta gttttgcttt tccttctcgg agcattcgca attctgcagt ttcagttgat
     1681 cgaaaaacgg gtgcactacc ggtgaggcgc attcgcgacc tgccgggccc gccggggctt
     1741 ccgcttctcg gcaacctgct gcagatcgac cgcacgcggc tgcacctcat cgccgaggcg
     1801 tggtgccgca cctatggcga ttacttccgc ttccgcatcg gcagccggga atacctcgtg
     1861 atagcggatc cggaagcgat cgccgcggtg ctgcgcgacc gcccggacgg ctttcagcgc
     1921 accgcgggcc tgaacgcagc cgcgcgcgag atgggcttcg gcggtgtctt ctccgcgaac
     1981 ggcgagcagt ggcgccgcca gcggccgatg gtgatggcga gcttcgaccc cgcgcacatc
     2041 aagtcgtact tcccggcgct ggtgagggtc acgcggcgat tcgccgggcg ctggcagcgc
     2101 gcggcggcgg ccggcggcac gatcgacctg caggccgacc tgatgcgcta cacggtggac
     2161 gtcacggccg ggctcgcctt cggcgcggac atcaacacgc tcgagtcgga ccgggacgtg
     2221 atccagagcc acctcgacaa ggtgctgccg gcgctgtttg cgcgcctgat ggcgccgttt
     2281 gtgctctggc gctggatcgg cgatctggcg ctcggccggc acctcgcggc attgcaccgc
     2341 gcggtcgacg gcttcatcgc cgccgcgcgg cagcgcatgg acgccgagcc gcggctgcgc
     2401 gcgcggccga ccaacctcat cgaggcgatg atcgccgcgc gcgataccga agcgagcggc
     2461 ctcgacgacg ccgacgtcgc cggcaacgtg ctgacgatgc tgctcgccgg cgaggacacc
     2521 accgcgaaca cgctggcgtg gatgatctac ctgctcgagc gcaatccggc ggcgatgcag
     2581 cgcgcgcgcg acgaggtgcg cgccgtcctc ggggcggagc cgctgccggc gcgctacgag
     2641 cagctctcgc gcctgccctt cgtggaagcc tgcgcgcacg agaccatgcg gctgaagccg
     2701 gtagctccca tcatcatcaa gcaggcggtg cacgataccg tcgtgg
//
LOCUS       DASPXW010000223         2802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1203685, whole genome shotgun sequence.
ACCESSION   DASPXW010000223 DASPXW010000000
VERSION     DASPXW010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1203685"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="VEL04_05245"
     CDS             <1..727
                     /locus_tag="VEL04_05245"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYT47803.1"
                     /translation="YTLLVKTGLGFALSAISAVVAVTLLSLVCYRLVIEPVREHEAAV
                     LIATIALALIFQELMLFSFGGHYLGIPSTLEGGIRVLGVSIPYQRMLILVVAAIMLTA
                     VWLLLYKTRLGLAIRATANDLEVANLMGMNVHRVAMVSVAISVALAAIAGVVVSPVFV
                     VDPFMWLAPLVTMLAIVVLGGLGSLKGSLIGALIIGYVEAITVFALPAGAYLKGAVAL
                     LIMVIVLLARPEGLFGVAFEEER"
     gene            727..1803
                     /locus_tag="VEL04_05250"
     CDS             727..1803
                     /locus_tag="VEL04_05250"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYT47804.1"
                     /translation="MYWLRKAWAELKNEVFVLPSRTLVLVWVLTLLALPALYGDPYVM
                     RILTMTCIFATFAASWDLLAGYTGQVNFGHALFFGAGAYTSALTSLKLGITPWATIWA
                     GAAVAMLFGLVVGYLCLRLRGSYLSLATLAFPLIALGLLFAFPDFSGGELGVSGLRRL
                     MPTPTGNYYVAAIAMLAVVFGLWALADSKFGIVLHAIRDDEVAARASGINTPRYKLAV
                     FALSAAAAGFAGALFAHYLRVAGPSTLEVALSFQVVIWGVFGGVATIYGPVAAVFILY
                     PLTEWLGSFRAFGELRLLIFAVIVLLVLLFMPRGLTPWVRDRIETQCPRCKQRNGIWK
                     KMCRLCGAALKAEVTRAQRLVGHQ"
     gene            complement(1756..2505)
                     /locus_tag="VEL04_05255"
     CDS             complement(1756..2505)
                     /locus_tag="VEL04_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT47805.1"
                     /translation="MPLLEVRELSLRYDKAQILNGVSLAVNHGEFVGLVGPNGAGKST
                     LLRALSGLVRFEERMKRGTAGEIVLEGEVRFAGERIDALPAHEIRRRGLAHCPERRRP
                     FRELTVLENLFAGAFLSKDSRQTKKRLEHCFALFPRLFERKQQIAGTLSGGEQQMLAT
                     ARLLMFEAKLIAIDEPSLGLAPKIREELFGAIGRIHAEGVALLLVEQEVGQVFRMASR
                     NYVLSQGRIIAEGSAQSLMADETLRAGYLGL"
     gene            complement(2492..>2802)
                     /gene="livG"
                     /locus_tag="VEL04_05260"
     CDS             complement(2492..>2802)
                     /gene="livG"
                     /locus_tag="VEL04_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Part of the ABC transporter complexes LivFGHMJ and
                     LivFGHMK involved in the high-affinity transport of
                     branched-chain amino acids; LivFGHMK is specific for the
                     transport of leucine, while LivFGHMJ is a transporter for
                     leucine, isoleucine, and valine; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HYT47806.1"
                     /translation="RAIATEPELLLLDEPLGGLNPAESELLARSVKRLHKGGRFGRLH
                     SEGPAVLMIEHKLKELMSIVDRVIVVDYGEIIADGPPAEVVKNPKVIEAYLGTQHAAA
                     "
BASE COUNT          385 a          927 c          936 g          554 t
ORIGIN      
        1 ctacacactg ctcgtcaaga ccggcctcgg cttcgcgctc tcggcgatca gcgcggtcgt
       61 cgcggtgacc ctgctctcgc tagtgtgcta ccgcctcgtc atcgagccgg tgcgcgagca
      121 cgaggcggcg gtgctgatcg cgaccatcgc gctcgcgctg atattccagg agctgatgct
      181 gttctccttc ggcgggcact acctcggaat tccgtcgacg ctcgagggcg ggatacgcgt
      241 cctcggcgtg agcatccctt accagcgcat gctgatcctg gtggttgccg cgatcatgct
      301 gaccgccgtg tggcttctgc tctacaagac gcggcttggc cttgcaatcc gcgccaccgc
      361 caacgatctc gaggtggcca acctgatggg catgaacgtc caccgcgtcg cgatggtgag
      421 cgtcgcgatc tcggtggcgc tggcggcgat cgccggcgtg gtggtgtcgc cggtgttcgt
      481 ggtcgatccg ttcatgtggc ttgcgccgct cgtcaccatg ctcgccatcg tcgtgctcgg
      541 cgggctgggc agcctcaagg gcagcctgat cggcgcgctc atcatcggct acgtcgaagc
      601 gatcacggtg ttcgcgcttc cggcgggcgc atacctgaag ggcgccgtgg cgctgctcat
      661 catggtcatc gttctgctcg cgcgtccgga agggctgttc ggcgtcgctt tcgaggaaga
      721 gcgctgatgt attggcttcg caaggcgtgg gccgagctga agaacgaggt tttcgtcctg
      781 ccttcgcgca cgctggtgct ggtgtgggtc ctgacgttgc tcgcgctccc cgccctgtac
      841 ggcgatccct acgtgatgcg cattctgacg atgacctgca tcttcgccac tttcgcggcg
      901 agctgggacc tgctcgcggg ctacaccggc caggtgaact tcgggcacgc gcttttcttc
      961 ggcgcgggtg cctacacctc ggcgctgacg tcgctcaagc tcggcatcac gccctgggcc
     1021 acgatctggg cgggcgccgc ggtggcgatg ctgttcggct tggtggtggg ttacctgtgc
     1081 ctcaggctgc gcggctcgta cctgtcgctc gccacgctcg cctttcctct catcgctctc
     1141 gggctgttgt tcgccttccc ggatttctcc ggcggcgagc tcggcgtttc cggcctgcgc
     1201 cgcttgatgc cgacgccgac cgggaactac tacgtcgcgg cgatcgcgat gctcgcggtc
     1261 gtgttcggcc tgtgggcgct cgcggattcg aagttcggca tcgtgctgca cgccattcgc
     1321 gacgacgagg tggcggcgcg cgcctcgggc atcaacacgc cgcgctacaa gctggccgtg
     1381 ttcgcgctct cggccgcggc ggcaggtttc gccggggcgc tgttcgcgca ttacctgcgg
     1441 gtcgccgggc cttcgacgct cgaggtcgcg ctgtcgttcc aggtcgtgat ctggggcgtc
     1501 ttcggcggcg tggcgacgat ctacgggccc gtcgcggcgg tgttcatcct gtacccgctg
     1561 accgagtggc tcggcagctt cagggccttc ggggaactgc ggctgctgat cttcgcggtg
     1621 atcgtgctgc tcgtgcttct cttcatgccg cgcgggctga cgccctgggt gcgcgacagg
     1681 atcgagacgc aatgcccgcg ctgcaaacaa agaaatggca tctggaaaaa aatgtgcaga
     1741 ctgtgcggcg ctgcgctaaa ggccgaggta acccgcgcgc agcgtctcgt cggccatcag
     1801 tgactgcgcg ctgccttcgg cgatgatcct gccctgagac agcacgtagt tgcggctcgc
     1861 catgcggaac acctggccga cttcctgctc gacgagcagg agcgcgacgc cctcggcatg
     1921 gatgcggccg atggcgccga acagctcctc gcggatcttc ggcgcgagcc cgagcgaggg
     1981 ctcgtcgatc gcgatcagct tcgcctcgaa catcagcaag cgcgcggtgg cgagcatctg
     2041 ctgctcaccg cccgagagcg tgccggcgat ttgctgcttt ctttcgaaca gcctcggaaa
     2101 cagcgcgaag caatgctcca gccgcttttt ggtttgccga gaatcttttg aaagaaaggc
     2161 gccggcaaac agattttcaa gcaccgtgag ctcgcggaag gggcgcctgc gctcggggca
     2221 gtgagcgagc ccgcgcctgc ggatttcatg agcggggagc gcgtcgatgc gttctcccgc
     2281 gaacctcacc tcgccctcga gcacgatctc gccggcggtg ccgcgcttca tgcgctcctc
     2341 gaacctcacc aggcccgaga gcgcgcgcag cagggtggac ttgccggcgc cgttcggccc
     2401 caccagcccg acgaactcgc cgtggttcac ggcgagcgac acgccgttca gtatctgcgc
     2461 cttgtcgtag cgcagcgaaa gctcgcgcac ctcaagcagc ggcatgttgg gtgcccaggt
     2521 aggcttcgat gaccttcggg ttcttcacca cctcggccgg cgggccatcg gcgatgatct
     2581 cgccgtagtc gacgacgatg acgcgatcga cgatcgacat cagctctttc agcttgtgct
     2641 cgatcatcag caccgcgggg ccctcggaat gcagccgccc gaagcggccg cccttgtgca
     2701 ggcgcttgac cgatcgcgcc agcagctcgg attcggcggg gttgagcccg ccgaggggct
     2761 cgtcgagcag cagcagctcg ggctcggtcg cgatggcgcg cg
//
LOCUS       DASPXW010000224         3260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_69203, whole genome shotgun sequence.
ACCESSION   DASPXW010000224 DASPXW010000000
VERSION     DASPXW010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69203"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1150)
                     /gene="glyS"
                     /locus_tag="VEL04_05265"
     CDS             complement(<1..1150)
                     /gene="glyS"
                     /locus_tag="VEL04_05265"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HYT47807.1"
                     /translation="MAKQAGNGVRAENLLVELLTEELPPKSLSLLGQFFADEVFNGLV
                     RQQLKQRDFAGRKIFASPRRLATFVPRVLAAAQDRSSEVPGPSVKAPPEAVAGFARKN
                     GVAVSALEQRDTPKGGIYVARVTLKGVTLDAILAEIVDETLKKLPTPKVMRWGNGDAR
                     FARPVHGLVMLHGNQVVPGRVLGLDSGNLTQGHRFLGKSPILLRHADEYEVRLGKDGW
                     VIADFAARREATERQLQAEATRQKADLGGYQGLLDEVTALVEYPQVYVGNFDASFLEV
                     PQECLILTMRQHQKYFPLFDAAGKLLPRFLIVSNMKVADPRPIVVGNERVVRPRLEDA
                     RFFYNQDRKQRLEERVPQLAKVVYHSRLGSQLERVERIQLLAGEIARKL"
     gene            complement(1154..2056)
                     /gene="glyQ"
                     /locus_tag="VEL04_05270"
     CDS             complement(1154..2056)
                     /gene="glyQ"
                     /locus_tag="VEL04_05270"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HYT47808.1"
                     /translation="MLTFQQLILQLNAFWDRHGCVLLQPYDMEVGAGTFHTATFLRAI
                     GPEPWNAAYVQPSRRPKDGRYGENPNRLQHYYQYQVVLKPSPLNIQDLYLDSLRKALG
                     IDPRKNDIRFVEDDWESPTLGASGLGWEVWLNGMEITQFTYFQQVGGLDCKPVLGEMT
                     YGLERLAMHLQNKESVFDLIWVDGVTYRDVYLQNEIEQSRYNFELANAEMLFRHFGDF
                     EAEAKRLVDAQCVLPAYEMVLKASHTFNLLEARGAISVTERAAYIGRVRTLARAVAQA
                     YYESREKLGFPMLKQAPATKKARA"
     gene            complement(2073..>3260)
                     /gene="lnt"
                     /locus_tag="VEL04_05275"
     CDS             complement(2073..>3260)
                     /gene="lnt"
                     /locus_tag="VEL04_05275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HYT47809.1"
                     /translation="IFPAAAGWLQARIPAPQAVRASLLIPASWTLFEWLRSWLLTGFP
                     WLTVGYAAADWPLQGYAPLAGVFGVSFITLSLAGLVSSIARGNRTRVSVAAVLALVIA
                     GEALRHVQWTAPAGAPLGVALLQGNVRQEMKFQPERYAHILETYARLVEGSRAELIVL
                     PETAVPRFYDLVDPAYLARLDAAARRNGGDLLLGVPYRNAPGEYYNSVITLGSSPRQA
                     YHKTHLVPFGEFVPPGFGWVMRLVEVPLADFSRGARNQPPLEVAKQRIAVNVCYEDAF
                     GDEIALRVPEATLLVNVSNVAWFGDSLAPAQHLQMAQFRAIETGRMHLAATNTGVTAA
                     IDRDGRLLARLPQYAEGRLEINAQGYSGATPYVLLQDWPILFISLVILASSVSVARRR
                     PSR"
BASE COUNT          566 a         1053 c         1070 g          571 t
ORIGIN      
        1 cgagcttgcg cgcaatttcg cccgccagca gctgaatgcg ctcgacgcgc tcgagctggc
       61 tgccaagcct actgtggtac accaccttcg ccaattgcgg cacgcgctcc tcgagccgct
      121 gcttgcggtc ctggttgtag aagaagcgtg cgtcctcgag tcgggggcga accacccgct
      181 cgttgcccac cactatcggg cgcgggtccg cgaccttcat gttcgagacg atcaggaatc
      241 tgggcagcag ctttcccgcc gcatcgaaca gcgggaaata tttctggtgc tgccgcatgg
      301 tgaggatcag gcactcctgc ggcacttcga ggaatgaggc gtcgaagttc ccgacgtaca
      361 cctgcgggta ttcgaccagc gcggtcacct cgtcgagcaa gccctggtac ccgccgagat
      421 ctgccttctg ccttgttgct tcggcttgca gctggcgctc ggtcgcttcg cggcgcgcgg
      481 cgaagtcggc gatgacccag ccgtccttgc ccagtctcac ctcgtattcg tcggcgtggc
      541 gcagcaggat cggtgatttg ccgaggaagc ggtgaccctg ggtgagattt cccgagtcga
      601 ggccaagcac gcgccccggc acgacctggt tgccgtgcag catcaccaag ccgtgcaccg
      661 ggcgcgcgaa acgcgcgtca ccgttgcccc atcgcatgac cttgggtgtt ggcagcttct
      721 tgagggtttc atcaacgatc tcggcaagaa tcgcatcgag agtgacgccc ttcagcgtca
      781 cccgcgcgac atagatcccg cctttaggcg tgtcgcgctg ctcgagggcg ctgacagcaa
      841 cgccattctt tctcgcgaac ccggccaccg cttcgggcgg cgccttgacc gacggccccg
      901 gcacttcgct tgatcggtcc tgcgccgccg cgagaaccct gggtacgaaa gtcgccaggc
      961 ggcgggggct ggcgaagatt ttccgaccgg caaagtcacg ctgcttcaac tgctggcgga
     1021 caaggccgtt gaacacttcg tctgcaaaga actgtccgag caaagaaagc gatttcggcg
     1081 gcagctcctc ggtcaggagc tcgacgagaa ggttctccgc gcgcacgcca ttcccggctt
     1141 gcttcgccat cgcctaggct cgggccttct tcgttgccgg cgcctgcttc agcatcggaa
     1201 agccaagctt ctcccgcgac tcgtaataag cctgcgccac ggcgcgggcg agggtgcgta
     1261 cgcggccgat gtaggccgcg cgttcggtga cggagatcgc gccgcgcgcc tcgagcaggt
     1321 tgaaggtgtg ggaggccttc aagaccatct cgtacgccgg aagtacgcac tgcgcatcaa
     1381 ccagacgttt tgcttcagct tcgaagtcgc cgaaatgcct gaacagcatt tcggcattcg
     1441 ccagctcgaa gttgtacctg gattgctcga tttcgttctg cagatatacg tcgcggtacg
     1501 taacaccatc cacccagatc aggtcgaaaa cgctttcctt gttctgcagg tgcatggcaa
     1561 ggcgctcgag gccgtaggtc atttcaccga ggaccggctt gcaatcgagc ccgcccacct
     1621 gctggaagta cgtgaactgg gtaatttcca tcccgttgag ccatacctcc caacccaggc
     1681 cggaggcgcc cagcgtcggc gattcccaat cgtcctcgac gaaccggatg tcattcttgc
     1741 gtgggtcgat gccgagggcc ttccttaagg aatcgaggta gagatcctgg atgttcagcg
     1801 gcgacggctt aagcaccacc tgatactgat agtagtgctg caggcggttc gggttctcgc
     1861 cgtagcgtcc gtccttgggc cggcgcgagg gctgcacgta agcagcgttc cacggctccg
     1921 gtccgatggc gcgcaggaaa gtcgcggtgt ggaaggtgcc ggcgccgacc tccatgtcgt
     1981 agggctgcag cagcacgcat ccatggcggt cccagaacgc gttgagctgc aggatcagct
     2041 gctggaaggt aagcatgaag cgtcgctgaa ttttaccggc tgggcctgcg tcgcgcgacg
     2101 gacacgctgc ttgccaagat aaccaatgaa ataaaaagta tcggccagtc ttgcagcagc
     2161 acgtacggcg ttgcgcccga gtaaccctgg gcgttgatct cgaggcggcc ctcggcgtac
     2221 tgcggcaagc gcgcgagcag ccgcccgtcg cggtcgatcg ccgcagtgac gccggtattg
     2281 gtggcggcga gatgcattcg tccggtctcg atggcgcgga actgcgccat ctgcaggtgc
     2341 tgcgcgggcg cgagcgagtc gccgaaccag gcgacgttcg acacgttgac cagcagcgtc
     2401 gcctcgggca cgcgcagcgc gatctcgtcg ccgaaggcgt cctcgtagca gacgttcact
     2461 gcgatgcgct gtttggcgac ctcgagcggg ggctgattgc gcgcgccgcg cgagaaatcc
     2521 gccagcggca cctcgaccag gcgcatcacc cagccgaatc ccggcggcac gaactcgccg
     2581 aacggcacca gatgcgtctt gtggtaggcc tgccgcggcg agctgccgag cgtgatcacg
     2641 ctgttgtagt actccccggg cgcgttgcgg taaggcacgc cgagcagcag gtcgccgccg
     2701 ttgcggcgtg cggcggcgtc gagccgcgcc aggtaggcgg ggtcgaccag gtcgtagaac
     2761 ctcggcaccg cggtctccgg aagcacgatc agctcggcgc gcgagccttc cacgaggcgg
     2821 gcgtaggtct cgagaatatg cgcgtagcgc tcgggctgga acttcatttc ctggcggacg
     2881 ttgccctgca gcagcgcgac gccgaggggc gcgccagcag gcgcggtcca ctgcacgtgc
     2941 cgcagcgctt cgcccgcgat gacgagggcg agcactgcgg cgaccgaaac gcgggtgcga
     3001 ttgccgcgcg cgatgctcga caccaggccc gccaggctga gcgtgatgaa cgagacgccg
     3061 aacacccccg cgagcggcgc atagccctgc agcggccagt cggccgcggc atagcctacg
     3121 gtgagccagg ggaagccggt gagcagccag ctgcgcagcc attcgaacaa ggtccaggag
     3181 gccgggatca gcaggcttgc gcgcactgcc tgcggcgccg ggatgcgcgc ctgcagccag
     3241 cccgcggccg cggggaaaat
//
LOCUS       DASPXW010000225         4953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_193727, whole genome shotgun sequence.
ACCESSION   DASPXW010000225 DASPXW010000000
VERSION     DASPXW010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4953
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193727"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..1837
                     /gene="secD"
                     /locus_tag="VEL04_05280"
     CDS             11..1837
                     /gene="secD"
                     /locus_tag="VEL04_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HYT47810.1"
                     /translation="MLYTLPNFFGESPAVQVSSAKSTVKVDAAVMARVQDALGKADTP
                     YTGAVLDATGLRLRFADTDTQLKAKDLIHGALNPGASPQATPEDDPNVSYVVALNLLS
                     ASPGWLTAIHALPMYLGLDLRGGVHFLLQVDMQAATAKRIESLAGDLRSQLRDKNIRH
                     GGVTREGQTLRVRFREAAMRDRARETVQANLPDLTLAEREDGQDYLLVGSLKPEALKR
                     NQDYALKQNITTLHNRINELGVAEPIIQQQGADRIVVQLPGVQDVARAKRLIGRTASL
                     EIRMVDEEKMMPDLLAAAARGQVPFGDEYYVERNGQPLLVKKQVVLTGDRLTDAQAGF
                     DQQTNEPAVHLRLDGQGSRIFQEVTRENVNKRMAILLIEKRKGEVVTAPVIRSEIPGG
                     RVQISGRMSTTEANDVALLLRAGSLAAPMEIIEERTVGPSLGRDNIDKGFNATRWGFL
                     AIVAFMSVYYALFGLISSAALGVNLLLLIALLSLMQATLTLPGIAAIALTLGMAIDAN
                     VLINERIREELRGGSTPQAAIAAGYERAWGTIFDSNVTTFIVGISLLMVPGAVRGFAV
                     VHCLGILTSMFSAVVVSRAIVNLTYGHRRRVAHLSIGDTAWK"
     gene            1855..2796
                     /gene="secF"
                     /locus_tag="VEL04_05285"
     CDS             1855..2796
                     /gene="secF"
                     /locus_tag="VEL04_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HYT47811.1"
                     /translation="MEFFKIRRTIPFMRHALIFNVLSAVTFLIAVFFLLHRGLNFSIE
                     FTGGTLIEVGYKQAANVDAIRRTLDQAGYQAEVQNFGTSREVLIRMHLEKNQSSADLS
                     QKVVAILKGEDQSAELRRVEFVGPQVGQELVEYGALALLVTAVGIVLYLWLRFEWRFG
                     LSGIIANLHDVVIILGCFAFFQWEFSLPVLAAVLAVLGYSVNESVVVFDRVRENFRKM
                     RKATTPEVIDSSITGVISRTIITHGCTQMMVSTMLIFGGPALHYFALALTIGICFGIY
                     SSVLVASPLVMWLGAKRDQFVRPVKRDRRDEHEGAVV"
     gene            complement(2819..4828)
                     /locus_tag="VEL04_05290"
     CDS             complement(2819..4828)
                     /locus_tag="VEL04_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HYT47812.1"
                     /translation="MIYAVSIFLSSFLLFLVQPLIARLILPWFGGSAAVWTTCMLFFQ
                     AMLLAGYAYAHFLTQKVAGKKLQALFHTALLAAALAALPIAPADAWKPAGEDEPVSRI
                     LLLLGATVGLPYFLLAATSPLLQAWYVRAYPGGNPYRLFAVSNLASLLALAGYPFVVE
                     PFLAAREQVSLWSWTFAGFALLCGAVAWMTPGQPVAEAATGEAPAISKTRFTLWLALS
                     ATGSAMLLAVTNHLTQNVASVPLLWLAPLTLYLASFILTFEGKGWYQPKYLWIPVLAG
                     LGAMAWLLADADYRFDLPLQLGIFLPGLFLGCLFCHGELYRLRPAPRHLTAFYLTVSA
                     GGALGGLAVAVAAPLVFSGYYELGVGLAVLALLAALRFAGLGFFARYLSLAALLGVTG
                     CAAYEGFRQQQDVRVATRSFYGVLRVKEYGSPGETTYLRRLVHGTIMHGEQYQHPSQR
                     TMLTTYYQPPSGIGAAILSRQGAPIRVGVIGLGTGTIAAYGRAGDVFRFYDIDPHVIE
                     LARSEFTYLADSAARVEIALGDARLTLEREPPQRFDVLAVDAFSSDAIPAHLITREAL
                     ATYLRHMKPDGIVAFHVSNRFLDLVPVVARIARENGAHAVLVRDDPEDDDPSQRSRSD
                     WVLVSRDPEALEAEKIVEARAVEAEDRPEWRTWTDDYSNLVQILK"
BASE COUNT          859 a         1666 c         1674 g          754 t
ORIGIN      
        1 cctcgccgcc ctgctctaca ccctgcccaa cttcttcggc gaatcgccgg cggtgcaggt
       61 ctcgagcgcg aaaagcacgg tcaaggtgga cgcagccgtc atggcgcgcg tgcaggacgc
      121 gctcgggaaa gcggacaccc cgtacaccgg agcggtgctc gacgccaccg gcctgcggct
      181 gcgatttgcc gataccgaca cgcagctcaa ggcaaaagac ctgattcacg gcgccctcaa
      241 cccgggcgca tcgccgcagg cgacgcccga ggacgatccc aatgtgagct atgtggtggc
      301 gctgaacctg ctctccgcct cgccgggctg gctcaccgcg atccacgccc tgccgatgta
      361 cctcggcctc gacctgcgcg gcggggtgca tttcctgctg caggtcgaca tgcaggccgc
      421 gaccgccaag cgcatcgaga gcctcgccgg cgacctgcgc tcgcagctgc gcgacaagaa
      481 catccgccac ggcggggtca cgcgcgaggg ccagacgctg cgcgtgcgct tccgcgaggc
      541 cgcgatgcgc gaccgggccc gcgagacggt acaggccaat cttcccgacc tgacgcttgc
      601 cgagcgcgag gacgggcagg actacctgct ggtcggcagc ctgaagcccg aggcgctcaa
      661 gcgcaaccag gactacgcgc tcaagcagaa catcaccaca ctgcataacc gcatcaacga
      721 gctgggcgtc gccgagccga tcatccagca gcagggcgcc gaccgcatcg tggtccagct
      781 gccgggagtc caggacgtgg cgcgcgcgaa gcgcctgatc ggccgcaccg cgagcctcga
      841 gatccgcatg gtcgacgagg aaaagatgat gccggacctg ctcgccgcgg cggcgcgcgg
      901 ccaggtgcct ttcggcgacg agtactacgt cgagcgcaac ggccagccgc tgctggtgaa
      961 aaagcaggtg gtgcttaccg gcgaccggct gaccgacgcc caggccgggt tcgaccagca
     1021 gaccaacgag cccgcggtgc acctgcggct cgacggccag ggctcgcgca tcttccagga
     1081 ggtgacgcgc gagaacgtca acaagcgcat ggcgatcctg ctgatcgaga aacgcaaggg
     1141 cgaggtggtc accgcgccgg tgatccgcag cgagatcccg ggcgggcgcg tgcagatctc
     1201 cggccgcatg agcaccaccg aggcgaacga cgtcgcgctg ctgctgcgcg cagggtcgct
     1261 cgcagcgccg atggagatca tcgaggagcg caccgtcggc ccgagcctcg ggcgcgacaa
     1321 catcgacaag ggcttcaacg ccacgcgctg gggctttctc gccatcgtcg ccttcatgtc
     1381 ggtctactac gcgctgttcg gcctgatctc gtcggcggcg ctgggtgtga acctgctgct
     1441 gctcatcgcg ctgctctcgc tcatgcaggc caccctcacg ctgccgggaa tcgcggccat
     1501 cgcgctcacg ctcggcatgg cgatcgacgc caacgtgctc atcaacgagc gcatacggga
     1561 ggaattgcgc ggcggatcga ctccccaggc cgccattgca gcaggctatg aacgcgcctg
     1621 gggcaccatc ttcgactcga acgtcaccac cttcatcgtc ggcatctcgc tcctgatggt
     1681 gccgggtgcg gtgcgcggct tcgccgtggt gcattgcctc ggcatcctga cctcgatgtt
     1741 ctccgcggtg gtggtgtcgc gcgccatcgt caacctcact tacggccacc gccggcgcgt
     1801 cgcccacctg tcgatcggcg acaccgcgtg gaaatgagcg cggcaatata gatcatggaa
     1861 ttcttcaaga tccgccgcac catcccgttc atgcggcatg cgttgatctt caacgtcctc
     1921 tcggccgtga cgtttctcat cgcggtgttc ttcctcctgc accgcgggct caacttctcg
     1981 atcgagttca ccggaggcac gctgatcgaa gtcggctaca agcaggctgc caacgtcgac
     2041 gccatccgcc gcaccctcga ccaggccggc taccaggccg aggtgcagaa cttcggcacc
     2101 tcgcgcgagg tgctgatccg catgcacctg gagaagaatc agtcgagcgc cgatctgtcg
     2161 cagaaagtgg tcgccatcct gaaaggcgag gaccaatcgg cggagctgag gcgcgtcgag
     2221 ttcgtcggcc cgcaggtcgg ccaggagctg gtcgagtacg gcgcgctcgc gctgctcgtc
     2281 accgccgtgg gtatcgtgct ctacctgtgg ctgcggttcg agtggcgctt cgggctctcc
     2341 ggcatcattg ccaacttgca cgacgtggtg atcatcctgg gctgcttcgc gttcttccag
     2401 tgggagttct cgctcccggt gctggcggcg gtgctcgccg tgctcggcta ctcggtgaac
     2461 gagtcggtgg tggtcttcga tcgagtacgc gagaacttcc gcaagatgcg caaggccacc
     2521 acccccgagg tcatcgacag ctccatcacc ggggtgatct cgcgcaccat catcacgcac
     2581 ggctgcacgc agatgatggt gagcacgatg ctcatcttcg gcggcccggc gctgcactac
     2641 ttcgcgctcg ccctcaccat cggcatctgc ttcggcatct actcctcggt gctggtggcg
     2701 agccccctcg tcatgtggct cggcgcgaag cgcgaccagt tcgtgcgccc ggtcaagcgc
     2761 gaccggcgcg acgagcacga aggcgcggtg gtttaaatgg ggacagaccc catttttctc
     2821 atttgaggat ctgcaccagg ttcgagtagt cgtcggtcca ggtgcgccac tcgggccggt
     2881 cctcggcctc gaccgcgcgc gcttccacga tcttttcggc ttcgagcgct tcggggtcgc
     2941 gcgacaccag cacccagtcg ctcctcgagc gctggctcgg gtcgtcgtcc tccgggtcgt
     3001 cgcgcaccag cacggcgtgc gcgccgttct cgcgcgcgat gcgggcgacg accggcacga
     3061 gatcgagaaa gcggttcgag acatggaacg cgacgatgcc gtcgggcttc atgtgccgca
     3121 ggtaggtggc gagcgcctcg cgcgtgatga ggtgcgccgg gatggcgtcg ctcgagaacg
     3181 cgtcgaccgc gagcacgtcg aagcgctgcg gcggctcgcg ctcgagcgtg agccgcgcgt
     3241 cgccgagggc gatctcgacg cgcgccgcgc tgtcggcgag gtaggtgaac tcgctccttg
     3301 cgagctcgat gacgtgcgga tcgatgtcgt agaagcggaa cacgtcgccg gcgcggccat
     3361 aggcggcgat ggtgccggtg cccagaccga tgacgccgac gcggatcggc gcgccctgcc
     3421 tggacaggat cgccgcgccg atgcctgaag gcggctggta gtaggtcgtg agcatggtgc
     3481 gctggctggg atgctggtat tgctcgccgt gcatgatggt gccgtgcacc agccggcgaa
     3541 gatacgtcgt ttccccgggc gagccgtatt ccttgacgcg cagcacgccg tagaagctgc
     3601 gcgtggcgac gcgtacgtcc tgctgctgcc ggaagccctc gtacgcagcg cagccggtaa
     3661 cgccgagcag cgccgcgaga cttagataac gcgcgaaaaa tcccagaccg gcgaagcgca
     3721 gcgccgcgag cagcgcgagc accgccaggc cgacgccgag ctcgtagtag ccgctgaaga
     3781 cgagcggcgc agccaccgcc acggcgaggc cgccgagcgc gccgccggcc gataccgtca
     3841 ggtagaacgc ggtgaggtgg cgcggcgccg gcctcagccg gtagagctcg ccgtggcaga
     3901 acaggcagcc gaggaacagc ccgggcagga agatgccgag ctgcagcggc aggtcgaagc
     3961 ggtaatccgc gtcggcgagc agccaggcca tggcgccgag cccggcgagc accgggatcc
     4021 acagatattt cggctggtac cagcccttcc cctcgaaggt gaggatgaag ctcgcgaggt
     4081 agagcgtgag cggcgcgagc catagcagcg gcaccgaggc gacgttctgc gtcaggtggt
     4141 tggtcaccgc gaggagcatc gccgacccgg tcgccgaaag cgcgagccag agcgtgaacc
     4201 gggtcttgct gatcgccggc gcctccccgg ttgctgcctc ggccacgggc tgtccgggcg
     4261 tcatccacgc caccgcgccg cagagcagcg caaaccccgc gaaggtccag gaccacaggc
     4321 tcacctgctc gcgcgccgcg agaaagggct cgacgacgaa cggatagccc gcgagcgcga
     4381 gcagcgatgc caggttcgag accgcgaaca ggcgataggg attgccgccg ggatacgccc
     4441 gcacgtacca ggcctgaagc agcgggctgg tcgcggcgag gaggaaatag ggcagcccga
     4501 cggtggcgcc gagcagcagc aggatccggc tgacgggctc atcctcgcct gcgggcttcc
     4561 acgcatcggc cggcgcgatc ggcagcgccg ccagcgccgc ggcgagcagc gccgtatgga
     4621 agagagcctg cagctttttc ccggcgacct tctgcgtcag aaaatgcgca taggcgtagc
     4681 cggcgagcag catcgcctgg aagaacagca tgcaggtcgt ccacacggca gcgctgccgc
     4741 cgaaccacgg caggatcagc cgtgcaatga gaggctgtac caggaacagc agaaacgacg
     4801 aaagaaaaat cgagactgcg taaatcaaac gcgttgcgcg agtttcgcgg ccaatcccgt
     4861 gtacttcgcc ggcgtcaggg cgagcaggcg cattttcgcc tcgggcggga gcggcaattt
     4921 cctgatgaac tgcgcaaggc tgcggcgatc gag
//
LOCUS       DASPXW010000226         3276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_948338, whole genome shotgun sequence.
ACCESSION   DASPXW010000226 DASPXW010000000
VERSION     DASPXW010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948338"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..155
                     /locus_tag="VEL04_05295"
     CDS             <1..155
                     /locus_tag="VEL04_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HYT47813.1"
                     /translation="GGVTIGPILLGAARPVHIVTPSATVRRMVNMTALVVADANAPHA
                     QEQSLF"
     gene            159..1343
                     /locus_tag="VEL04_05300"
     CDS             159..1343
                     /locus_tag="VEL04_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HYT47814.1"
                     /translation="MEPASKVAPFPANRPKPRAGLVLTGGGARAAYQVGVVKAVRDIL
                     GSPVRNPFPIVCGTSAGAINAATLAVFADNFSRGVANLLEVWEHMHCDQIYHTDPWRI
                     MKTGARWLGALMLMSRRNPVSLLDNQPLREMLGKNLDFGKIQGHIDSGALYAVCVTAS
                     GYTTGQSVSFFQGGSGLEGWERNQRIGAAVPLQLELLLASAALPFIFPAVKVHREYFG
                     DGSMRQIAPVSPALHLGADRVLIVGTGRQAPEQARARSNVYPSLAQVAGHALNSIFLD
                     SLMVDIERMERINRTVKLIPPDKLSDSTIQLRAVKVLFIAPSQPLERIAARFIHELPA
                     TVRFILRPTGALNRSGSNLASYLLFEESFCRALIDLGYQDTISRESEVKEFFEPEETA
                     YG"
     gene            1364..1621
                     /locus_tag="VEL04_05305"
     CDS             1364..1621
                     /locus_tag="VEL04_05305"
                     /inference="COORDINATES: protein motif:HMM:NF043076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhaM family polyhydroxyalkanoate granule
                     multifunctional regulatory protein"
                     /protein_id="HYT47815.1"
                     /translation="MADDKPPQDPFEMFRRLWGPLGVPLPGMAVPTFDPQEVEKRIAE
                     LRSVEAWLNMNLNMVRFSIQGLELQRAALQAMRGAEPKKEP"
     gene            1646..2617
                     /locus_tag="VEL04_05310"
     CDS             1646..2617
                     /locus_tag="VEL04_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003275776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HYT47816.1"
                     /translation="MPKAVRYHKQGGPEVLQFEDVPVGEPGQGQVRVRHTAIGVNFVD
                     TYQRSGLYPMQLPQVAGNEGAGVVDAVGPGVTDLKKGDRVAYTGLPGSYCESRVVPAD
                     RLVKLPQGISEEQAASMLLKGLTVHYLIHTTYPVKKGETVLWHAAAGGVGTIACQWLK
                     ALGVSVIGTVGSDDKAKLAKEHGATHVINYSKENFVERVKALTGGKGVPVVYDGVGKA
                     TWEGSLDCLRPRGLIASFGNASGPVPPVNLGILSAKGSLYVTRPTLATHIASRADLVE
                     RSNSLFDAVKSGKVKIETTERYKLSDAQSAHRDLEGRKTTGSVVLTP"
     gene            complement(2624..2863)
                     /locus_tag="VEL04_05315"
     CDS             complement(2624..2863)
                     /locus_tag="VEL04_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47817.1"
                     /translation="MKRPALLVALAFASSLAFANNCPNEMKAIDAALPKAKLDAKQTA
                     EVKKLRAQGEQLHKAGKHSESMAALGKAKGILGIK"
     gene            2909..>3276
                     /locus_tag="VEL04_05320"
     CDS             2909..>3276
                     /locus_tag="VEL04_05320"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HYT47818.1"
                     /translation="MPATRQTIAHGDLFRVRATQIPGGTIELDLDELRRRLRQRRAAA
                     FSVYGDDGARREEAAATAAAVLVPIVAHPAELTVLFTQRTSQLRSHSGQVSFPGGRAE
                     PEDASPEFTALREAEEEIGLP"
BASE COUNT          548 a         1107 c         1091 g          530 t
ORIGIN      
        1 gcggcggcgt gaccatcggc ccgatcctgc tcggcgccgc acgcccggtg cacatcgtca
       61 ccccctcggc caccgtgcgc cgcatggtga acatgaccgc cctggtggtc gccgacgcga
      121 atgcgccgca cgcccaggag cagagcctct tctagccgat ggaacccgcc agcaaggtcg
      181 cgccttttcc tgccaaccgg ccgaagccgc gcgccggcct ggtcctcacc ggcggcggcg
      241 cgcgcgccgc ctaccaggtc ggggtggtga aggcggtgcg cgacattctc ggcagcccgg
      301 tgaggaaccc gttcccgatc gtctgcggca cttcggcggg tgcgatcaac gcggcgaccc
      361 tcgcagtgtt cgccgacaat ttctcgcgtg gggtcgccaa cctgctcgag gtgtgggagc
      421 acatgcactg cgaccagatc taccacaccg atccctggcg catcatgaag accggcgcac
      481 gctggctcgg cgccttgatg ctgatgtcgc gccgcaaccc ggtctcgctg ctcgacaacc
      541 agccgctgcg cgagatgttg ggaaaaaatc tcgattttgg aaaaattcag ggtcatatcg
      601 attcgggtgc gctgtatgcc gtgtgcgtca ccgcatcggg ctacaccacc gggcagagcg
      661 tgtcgttctt ccagggcggc agcgggcttg agggctggga gcgcaaccag cgcatcggcg
      721 ccgcggtgcc gctgcagctc gagctgctgc tcgcctcggc cgcgctgcct ttcatcttcc
      781 cggcggtcaa ggtgcaccgc gagtatttcg gcgacggctc gatgcgccag atcgcgccgg
      841 taagcccggc gctgcacctc ggcgccgacc gggtgctgat cgtcggcact ggccgccagg
      901 ccccggagca ggcgcgcgcg cgctccaacg tctacccttc gctcgcgcag gtcgccggtc
      961 acgcgctgaa cagcatcttc ctcgacagcc tgatggtcga catcgagcgc atggagcgca
     1021 tcaaccgcac cgtgaagctg atcccgcccg acaagctctc ggactcgacc atccagctgc
     1081 gcgcggtgaa ggtgctgttc atcgccccct cgcagccgct cgagcgcatc gcggcgcgct
     1141 tcatccacga gctgccggcg acggtgcgct tcatcctgcg tcccaccggc gcgctgaacc
     1201 gctcgggctc caacctggcg agctacctgc tgttcgagga gtccttctgc cgcgcgctca
     1261 tcgacctcgg ctaccaggac accatcagcc gcgagtccga ggtgaaggaa ttcttcgagc
     1321 cggaggaaac agcctatggg tgacaagaag ccgtccgaac atcatggctg acgacaagcc
     1381 gcctcaggac ccgttcgaga tgttccgccg cctgtggggg ccgcttggcg tgccgctccc
     1441 cggcatggcg gtgccgacct tcgatccgca ggaggtcgag aagcgcattg ccgagctgcg
     1501 ctcggtcgag gcctggctca acatgaacct caacatggtc aggttctcga tccaggggct
     1561 cgaattgcag cgcgccgccc tgcaggcgat gcgcggagcg gagcccaaga aagagccatg
     1621 aaatcttttt caccaggaga acgcaatgcc taaagcagtc cgctaccaca agcaaggcgg
     1681 cccggaagta ctccagttcg aggacgtccc ggtcggcgag ccgggccagg gccaggtgcg
     1741 cgtgcgccac accgccatcg gcgtcaattt cgtcgacacc tatcagcgct cggggctcta
     1801 tccgatgcag ctgccgcagg tcgcgggcaa cgagggcgcg ggggtggtcg acgcggtcgg
     1861 cccgggcgtg accgacctga agaaaggcga ccgcgtcgcc tataccggcc tgccggggtc
     1921 gtactgcgag tcgcgcgtgg tgccggcaga tcgcctggtg aagctgccgc aggggatcag
     1981 cgaagagcag gccgcctcga tgctgctgaa ggggctcact gtgcattacc tcatccacac
     2041 cacgtacccc gtcaagaagg gcgagacggt gctgtggcat gccgcggcgg gcggcgtcgg
     2101 caccatcgct tgccagtggc tcaaggcgct cggcgtcagc gtgatcggca cggtggggtc
     2161 cgacgacaag gcgaagctgg ccaaggaaca cggcgcgacg cacgtcatca actactcgaa
     2221 ggagaacttc gtcgagcgcg tcaaagcgct caccggcggc aagggcgtgc cggtggtcta
     2281 cgacggggtg ggcaaagcga cttgggaagg ctcgctcgac tgcctgcgtc cacgcggcct
     2341 gatcgcgtcg ttcggtaacg cctcggggcc ggtgccgccc gtgaatctgg gcatcctttc
     2401 ggccaaaggc tcgctctacg tcacgcggcc gacgctcgcc acgcacatcg ccagccgcgc
     2461 cgacctcgtc gagcgctcca actcactgtt cgatgcggtg aaatcgggca aggtgaagat
     2521 cgagaccacg gagcgctaca agctctccga cgcccagagc gcgcaccgcg acctcgaagg
     2581 gcgcaagacg accgggtcgg tcgttctcac gccctagaaa acgctacttg atgccgagga
     2641 tgcctttcgc cttgccgagg gccgccatcg actcggagtg cttgccggcc ttgtgaagct
     2701 gttcgccctg ggcgcgcagc ttcttgactt cagcggtctg cttggcgtcg agcttggcct
     2761 tcggcagcgc ggcatcgatc gccttcatct cgttcgggca gttattggcg aacgcgagcg
     2821 aagacgcgaa cgctagggcg actaacaggg cggggcgttt catgttcttc cttgggtggt
     2881 tgtcggggga aaccgtagcc agcctaacat gccggctacg aggcaaacaa ttgcacatgg
     2941 agacctattc cgggtgcgcg ccacccagat ccctgggggg acgatcgaac tcgatctgga
     3001 cgaattgcgc cggcgcttga ggcagcgccg cgccgcagcc ttttcggttt acggcgacga
     3061 cggcgcgcgc agggaggagg cggcggcgac cgccgccgcg gtgctggtgc cgatcgtcgc
     3121 ccatccggcc gagctcacgg tgctgttcac gcagcgcacc tcgcagctgc gcagccattc
     3181 cggccaggtg agctttcccg gcgggcgcgc cgagccggaa gacgccagcc cggagttcac
     3241 cgcgctgcgc gaggccgagg aggagatcgg gctgcc
//
LOCUS       DASPXW010000227         4699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1078246, whole genome shotgun sequence.
ACCESSION   DASPXW010000227 DASPXW010000000
VERSION     DASPXW010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4699
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1078246"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VEL04_05325"
     CDS             complement(<1..225)
                     /locus_tag="VEL04_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47819.1"
                     /translation="MSQTYVFARNAPDAEYDRALAWAALLLAATGLVMVYSSSIATAE
                     ASRYTGNNAAWYLARHGVFLATALGAAITVF"
     gene            complement(222..1601)
                     /gene="murD"
                     /locus_tag="VEL04_05330"
     CDS             complement(222..1601)
                     /gene="murD"
                     /locus_tag="VEL04_05330"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HYT47820.1"
                     /translation="MREPAFTQLPRPAPMPLAGKRVLVLGLGDTGLAVARWVERAGGE
                     PRVADTRSAPPRRSEFSGELHTGAFTTALLEGIDLVCISPGLPLADPLVQSAVERRVP
                     VCGDIELFAWHVHATGPCRVVAVTGTNGKSTVTSLTGHLLRTAGIDCEVAGNIGPPAL
                     DALIRRAVPPAAWVLELSSYQLETTWSLEPDVGAMLNLSEDHLDRYASLDEYAAAKAR
                     IFIGRGTQVLNRDDPRSRSLAIAGRKRVSFGLDAPPAAEDWGLVQINGSKWLAQGANA
                     VLEASALPIHGWHNAANALAACALARAAGARYEALVEGLQTFRGLPHRLERIAVRRGV
                     EWYDDSKGTNVGATDAALRGLDRKTVLILGGEGKGQDFSSLRESVAHFARCVLLIGRD
                     APLIEAALAGLPVEKCSSLEDAVQRAAELSHPGDAVLLSPACASFDMFRDYRHRGDAF
                     AAAVGRLAP"
     gene            complement(1601..2692)
                     /gene="mraY"
                     /locus_tag="VEL04_05335"
     CDS             complement(1601..2692)
                     /gene="mraY"
                     /locus_tag="VEL04_05335"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HYT47821.1"
                     /translation="MLLELAQWLAKDVRLFNVFNYITLRAVLACLTALAVSLVLGPYM
                     IRKLTAYKIGQAVRDDGPRSHLTKAGTPTMGGALILVSICVTTLLWGDLSNRMLWVVL
                     LVTLAFGTIGWVDDYRKVVQGNPKGLSARPKFLWQSLIGVAAACYLAYLAQTMPALNQ
                     LIVPFFKFIAYPLGTLGFIVLTYFVIVGTSNAVNLTDGLDGLAIMPTVMIGSALGIFA
                     YVAGHAVFSKYLGLPYIPGAGELTVICGALAGAGLGFLWFNAYPAEVFMGDVGALALG
                     AALGTVAVIVRQEIVLFIMGGVFVAETLSVMIQVLYFKWTGGKRIFRMAPLHHHYELE
                     GWKESQVVVRFWIITMMLVLFGLSTLKLR"
     gene            complement(2692..4047)
                     /gene="murF"
                     /locus_tag="VEL04_05340"
     CDS             complement(2692..4047)
                     /gene="murF"
                     /locus_tag="VEL04_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HYT47822.1"
                     /translation="MMSIAQAAMALGVRSSGPDTVFTGVSSDTRTLKTGDLFVALRGE
                     RFDGHGFLDAAKAAGAAAAMVERRYKGPYPLPVIVVEDSKLALGDLASRWRSRFAPVL
                     VAVTGSNGKTTVKEMLASILRSHAGEQAVLATAGNLNNDIGLPLTLLGLREAHRYCAV
                     ELGMNHKGEIAYLAAIAQPTVALVNNAQREHLEFMQSVDEVAAENASVYARLPADGVA
                     VVNVDDAHAGFFRQAAGTRRVMDFSLDTGAAVTGGYTLKQLSSEIRIKTTDGEAAATL
                     AIPGLHNVRNALAAAACACAAGIAVQTIAAGLSRFRPYGGRLQVKQARDGATLVDDSY
                     NANPDSVRAAIDVLASCPGPTVLVLGDMGEVGSQGAEFHREVGAYARDKGISRLLALG
                     EATRHAVAAFGSGAQHFQHLEELVAAVSGRTVLVKGSRFMKMERVVAALTGAAAAAGS
                     H"
     gene            complement(4044..>4699)
                     /locus_tag="VEL04_05345"
     CDS             complement(4044..>4699)
                     /locus_tag="VEL04_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HYT47823.1"
                     /translation="ARVIEEPRVELESSWGSATASVPALGRFNVANALGVLGCLVARG
                     IPFHEAAALLGQIPPVPGRMQRVAERPLVVIDYAHTPDALEKVLQALRPLADARGGKL
                     VAVFGAGGDRDPTKRALMGAIAARFAGRVVLTSDNPRSEDPLAIIEAIRGGADGRCEI
                     EPDRRRAIEQAIVEAALGDVVLVAGKGHENYQEIAGRRLPFSDLAVAGGALERRVKQ"
BASE COUNT          784 a         1639 c         1568 g          708 t
ORIGIN      
        1 gaaaacggtg atcgccgcac cgagcgcggt cgcgagaaat acgccgtggc gcgccaggta
       61 ccacgcggca ttgttcccgg tgtagcggct cgcctcggcc gtggcgatcg aggacgaata
      121 caccatgacg agcccggtcg ccgcgagcag cagcgcggcc caggcgagcg cgcggtcgta
      181 ttccgcgtcc ggcgcgttgc gcgcgaaaac gtaggtctgg ctcacggcgc cagcctgccc
      241 accgcggcgg caaacgcatc cccgcgatga cggtaatcgc gaaacatgtc gaagctggcg
      301 caggcaggcg agagcagcac cgcgtcgccc ggatgcgaaa gttccgctgc ccgctgcacc
      361 gcgtcctcga gactcgagca tttttcgacc ggcagcccgg cgagcgctgc ctcgatgagc
      421 ggcgcgtccc gtccgatcag cagaacgcag cgagcgaagt gcgcgacgct ttcccgcagg
      481 gacgagaaat cctgtccctt gccctcgccg cccagaatca gcaccgtttt cctgtcgaga
      541 ccgcggagtg ctgcgtcggt cgcgccgacg ttggtgcctt ttgaatcgtc gtaccactcg
      601 acaccgcgcc gcaccgcgat gcgctcgagg cggtgcggca acccgcggaa tgtctgcagc
      661 ccctcgacca gcgcttcgta gcgtgcaccg gccgcgcgcg cgagcgcgca agccgcgagc
      721 gcgttggcgg cgttgtgcca gccgtgaatc ggaagcgcgg acgcctcgag aactgcattg
      781 gcgccctgcg caagccattt cgacccgtta atctggacca agccccaatc ctcggcggcc
      841 ggcggcgcgt cgaggccgaa gctcacccgc tttctaccgg cgattgcaag tgacctcgat
      901 cgcgggtcgt cgcggttgag cacctgcgtg ccccgtccga taaagatgcg cgccttcgcg
      961 gcggcgtatt cgtcgaggct cgcgtagcga tcgagatggt cctcgctcag gttgagcatg
     1021 gcgccaacgt cgggctcgag cgaccaggtg gtttcaagct ggtagctcga cagctccagc
     1081 acccacgccg caggcggcac cgcccgccgt atcagcgcgt cgagcgccgg cggcccgatg
     1141 tttcccgcga cctcgcagtc tatgcctgct gtgcggagca ggtgtccagt gagcgaggtc
     1201 accgtgctct tgccgttggt tccggtcaca gcgaccaccc ggcaggggcc ggtcgcgtgc
     1261 acatgccatg cgaacaactc aatgtcgccg cagaccggga cgcgccgttc cacggcgctc
     1321 tgaacgagag gatcggcaag cggcaggcct ggactgatgc agaccaggtc gatgccctcg
     1381 agcagcgccg tggtgaacgc gccggtgtgc aactcgcctg aaaattcgct gcgccgcggc
     1441 ggcgcgctgc gcgtatcggc gacgcgcggc tcgccgcccg cgcgctcgac ccagcgcgcc
     1501 accgcgagcc cggtgtcgcc cagcccgagg accagcaccc gcttgccggc aagcggcatc
     1561 ggggccggtc tgggaagctg cgtgaatgcg ggctcgcgca tcatctgagc ttgagggtcg
     1621 acaggccgaa caacaccagc atcatggtga tgatccagaa gcgcaccacc acctggcttt
     1681 ccttccagcc ctcgagctcg tagtggtggt gcagcggcgc catgcggaag atgcgcttgc
     1741 cgccggtcca cttgaaatac agcacctgga tcatcacgga gagcgtctcg gcgacgaaca
     1801 cgccgcccat gatgaacagc acgatctcct ggcgcacgat cacggccacc gtgccgaggg
     1861 cggcgccgag ggcaagcgcg ccgacgtcgc ccatgaagac ttcggctgga taggcattga
     1921 accagaggaa gccgagcccg gcgccggcta gcgcgccgca gatcaccgtg agctcgcccg
     1981 cccccggaat gtacggcagg ccgaggtatt tcgagaacac ggcatgtccc gccacgtagg
     2041 caaagatgcc gagcgcgctg ccaatcatca ccgtcggcat gattgccagc ccgtcgaggc
     2101 cgtcggtgag attgaccgcg ttggaggtgc cgacgatcac gaagtacgtg agcacgatga
     2161 atcccagcgt gccgagcgga taggcgatga acttgaagaa cggcacgatc agctggttca
     2221 gcgccggcat ggtctgggcg aggtaggcga ggtagcaggc ggccgcgacg ccgatgagcg
     2281 attgccagag aaacttcggg cgcgcggaca atcccttcgg attgccttgc actaccttgc
     2341 ggtaatcgtc gacccagccg atggtgccga acgcgagcgt cacgagcagc accacccaga
     2401 gcatgcggtt ggagagatcg ccccagagca gcgtcgtgac gcagatcgag accaggatca
     2461 gcgcgccgcc catggtcggc gtgcccgcct tggtgaggtg cgagcgcggg ccgtcgtcgc
     2521 gcaccgcctg cccgatcttg tacgcggtca gcttgcggat catgtacggg ccgagcacca
     2581 gcgagaccgc gagggcggtg aggcaggcga gcacggcgcg cagcgtgatg tagttgaaga
     2641 cgttgaacag ccgcacgtcc ttggcaagcc attgggcgag ctcgagcagc actagtgact
     2701 ccctgcggcg gccgcagcgc cggtgagcgc ggcgaccacc cgctccatct tcatgaaccg
     2761 cgagcctttc accagcaccg tcctgccgct gacggcggcg accagctctt cgagatgctg
     2821 aaagtgctgc gcgcccgagc cgaacgccgc caccgcgtgc cgcgtcgcct cgcccagcgc
     2881 gagcagcctg gagatcccct tgtcgcgcgc gtaggcgccc acttcgcggt gaaactccgc
     2941 gccctgactg ccgacctcgc ccatatcacc cagcaccagc acggtcggcc cggggcacga
     3001 ggcgagcacg tcgatcgcgg cccgcaccga gtcgggattg gcgttgtagc tgtcgtcgac
     3061 cagcgtggct ccgtcccgcg cctgcttcac ctgcaagcgg ccaccatacg gacgaaagcg
     3121 cgacagccct gccgcgatcg tctgcacagc gatgccggcg gcgcaggcgc aggccgccgc
     3181 agcgagcgcg ttccgcacgt tgtgcaggcc tggtatggcg agcgtggcgg ccgcttctcc
     3241 gtcggtggtc ttgatgcgta tttcgctcga aagctgcttc agcgtgtagc cgccggtaac
     3301 ggcggcaccg gtatcgagcg agaaatccat gactcgccgg gtcccggcgg cctggcggaa
     3361 aaagccggca tgcgcatcgt ccacgttcac caccgcgaca ccgtccgcgg gcagcctggc
     3421 atacacgctg gcgttctcgg ccgcgacctc gtccaccgac tgcatgaact cgaggtgctc
     3481 gcgctgcgca ttgttgacca gcgccaccgt cggctgcgcg atggcggcga gatacgcgat
     3541 ttcgcccttg tgattcatgc cgagctcgac tgcgcagtag cggtgcgcct cgcgcaggcc
     3601 aagcaaggtg agcggcaagc cgatatcgtt gttcaggttg cccgccgtgg cgagcaccgc
     3661 ctgctcgccc gcgtgactgc gcaggatgga tgccagcatc tccttcaccg tggtcttgcc
     3721 gttagagccg gtgaccgcga ccagcacggg cgcgaagcgc gagcgccagc ggctcgcgag
     3781 gtcgccaagc gcgagcttgc tgtcctcgac gacgatcacc gggagcggat aaggaccctt
     3841 gtagcgacgc tcgaccatgg ccgccgctgc gcccgcggcc ttcgctgcgt cgagaaagcc
     3901 gtgtccgtcg aagcgctcgc cgcgcaacgc cacgaacaga tcgcccgtct tgagcgttct
     3961 ggtgtcgctc gacacgccgg tgaagaccgt atccggtccg gagctgcgca cgccgagcgc
     4021 catggcggcc tgtgcaatgc tcatcattgc ttcactcgcc gctcgagtgc cccgcccgcc
     4081 acggccaggt cggaaaacgg caggcgccgt cccgcgatct cctggtagtt ttcgtggccc
     4141 tttccggcga ccagcacgac gtccccgagg gccgcctcga caatggcttg ctcgatcgcg
     4201 cggcggcggt cgggctcgat ctcgcagcgg ccgtccgcgc cgccgcggat cgcctcgatg
     4261 atggcaagcg ggtcctcgct gcgcggattg tccgaggtca gcaccacgcg gccggcgaag
     4321 cgcgcggcga tcgcccccat cagcgcacgc ttggtcggat cgcgatcacc gccggcgccg
     4381 aataccgcga ccagctttcc gccgcgcgca tcggccagcg gacgcagcgc ctgcagcacc
     4441 ttctcgagcg cgtccggggt gtgcgcgtag tcgatcacca cgagcggtcg ctccgcgacc
     4501 ctctgcatgc ggccggggac cggcgggatc tggccgagca gcgcggcggc ctcgtgaaac
     4561 ggaatgccgc gcgccaccag gcagccgagc acgccgagcg cgttggcaac attgaagcgt
     4621 ccgagcgcag ggacgctcgc agtcgcgctt ccccagctcg attcgagctc cacccgcggc
     4681 tcttcgatca cgcgcgcga
//
LOCUS       DASPXW010000228         4483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_948432, whole genome shotgun sequence.
ACCESSION   DASPXW010000228 DASPXW010000000
VERSION     DASPXW010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948432"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..816)
                     /locus_tag="VEL04_05350"
     CDS             complement(130..816)
                     /locus_tag="VEL04_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HYT47824.1"
                     /translation="MLDTTRRVATPEGIELTLRLAGPVPRALAWAIDFALRAAAVFAV
                     MMVALPLGHAGTGLALIAAFFAEWLLPAWFEASWRGQTPGKRALGLAVLNDDGTPVRW
                     PAALTRNLLRAVDFLPLLYGTGLVTMLASRDFKRLGDLAAGTVVVYQSEKSIQERKFP
                     DAQPIAPPFHLDLEEQRAVLELAERSASLTRERLEELAELPGPLVGRLGGAHAAARLI
                     GMANYLLGRK"
     gene            complement(1011..2270)
                     /gene="rho"
                     /locus_tag="VEL04_05355"
     CDS             complement(1011..2270)
                     /gene="rho"
                     /locus_tag="VEL04_05355"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HYT47825.1"
                     /translation="MHLSELKSHHVTQLVEMAVSNGIDGANRLRKQDLIFALLKNQAK
                     KGESIFGDGTLEVLPDGFGFLRSPETSYLASTDDIYVSPSQIRRFNLHTGDSIEGEIR
                     TPKDGERYFALVKVDKVNGEVPEAAKNKILFENLTPLHPDEAFKLERDIKAEENITGR
                     VIDIVAPIGKGQRGLIVSPPKAGKTVMLQHIAHAIVANHPEVVLIVLLIDERPEEVTE
                     MSRSVRGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALQKPKRFFGAARHVEEGGSLTIIATALIDTGSRMDDVIYEE
                     FKGTGNMEIHLDRRMHEKRIYPAINVNRSGTRREELLIEQPILQKVWVLRKLLFPMDE
                     LEAAEFLIDKLKATKSNAEFFDSMRRG"
     gene            complement(2481..2810)
                     /gene="trxA"
                     /locus_tag="VEL04_05360"
     CDS             complement(2481..2810)
                     /gene="trxA"
                     /locus_tag="VEL04_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HYT47826.1"
                     /translation="MSSANIRHVTDDTFDPEVLKSETPVLVDYWAEWCGPCKSIAPIL
                     DELAREYEGRLKIAKINVDENQAVPAKFGIRGIPTLMLFKNGNVEATRVGALSKSQLT
                     AFLDSNI"
     gene            2906..3229
                     /gene="fdxA"
                     /locus_tag="VEL04_05365"
     CDS             2906..3229
                     /gene="fdxA"
                     /locus_tag="VEL04_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HYT47827.1"
                     /translation="MTYVVTESCIKCKYTDCVDVCPVDCFREGPNMLVIDPDECIDCT
                     LCVPECPVEAIFAEDDVPDAQKEFTALNAELSRLWKPIIERKPAPPDADDWAKTKEKK
                     HLLER"
     gene            3336..3575
                     /locus_tag="VEL04_05370"
     CDS             3336..3575
                     /locus_tag="VEL04_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HYT47828.1"
                     /translation="MEQARLAFTAVYLKSNSGYIGFIEELPGVNSHGRTLDEARATLQ
                     KLAAVVFDEERREAEELIAGKDVVRESFFVPIPRP"
     gene            3664..>4483
                     /locus_tag="VEL04_05375"
     CDS             3664..>4483
                     /locus_tag="VEL04_05375"
                     /inference="COORDINATES: protein motif:HMM:NF015558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HYT47829.1"
                     /translation="MAAKSWRTEDWVAVYLGFFVIAVVLAAFSWKWFDLGVASSTFRW
                     TADAQIAGSTPGWRAALDDVAKEARAKGRKELAARAGGLKAALDKGERKGIEKAAGDL
                     SKAGGRNTVAGTLGSEMRGHVAATADKVFSAGNLGKVLTVGIALLVIGAAGFALIGGN
                     VGAFLIGFPAVFVLAWLARVIAGNGLFIDYGVEYVIFALVMGLLVSNTIGTPKWLRAA
                     VQTEYFIKTGLVILGAGLLFLEVIQAGVLGILQAVLVVFVIWYVCFWFARKLRVD"
BASE COUNT          720 a         1402 c         1533 g          828 t
ORIGIN      
        1 cggtagcgca gcggcatctc cgccaggtcg aacgcaggct ttttcggctg ctcgaggaat
       61 ttctcgaact cctgccactc gcgctcgtgc acggtctcga actggcgctg cctcatttgc
      121 gtccctgcct catttgcgtc ccaacaagta gttggccatg ccgatgaggc gcgccgcggc
      181 gtgcgcgccg ccgagccggc cgacgagcgg gcccggcagc tcggcgagct cttcgagccg
      241 ctcgcgcgtc aggctggcgg agcgctcggc gagctcgagc accgcgcgct gctcttccag
      301 gtcgaggtgg aagggcggcg caatcggctg cgcgtcgggg aacttgcgtt cctggatgct
      361 tttttcggac tggtagacca ccacggttcc cgcggcgagg tcgccgagcc gcttgaagtc
      421 gcggctggcg agcatcgtga cgaggccggt gccgtacagc aggggcagga aatcgaccgc
      481 gcgcagcagg ttgcgggtga gcgcggccgg ccagcgcacg ggcgtgccgt cgtcgttcag
      541 caccgcgagg ccgagggcgc gcttgcccgg ggtctggccc cgccagctcg cctcgaacca
      601 tgccggcagc agccactcgg cgaagaacgc ggcgatcagc gcgagcccgg tgccggcgtg
      661 cccgagcggc agcgccacca tcatcaccgc gaacaccgcc gcggcgcgca gcgcgaaatc
      721 gatcgcccag gcgagcgcgc gcggcaccgg cccggcgagg cgcagggtca gttcgatgcc
      781 ttcaggtgtt gcgactcgac gcgtggtgtc gagcatgcgg ctcccctccc cggggaaacc
      841 ggattctaaa tgaccccctg ataaggggtc gtgagccgaa ggcgagcggg gggttggttt
      901 tgaaaaaaac cgggacctga actgaaaccc cccggccctt cgggccaccc cccttgtcag
      961 gggggcaaac ccttgtcagg ggggcaaacc cttgtcaggg gggcaaaccc ttatccccgc
     1021 cgcatcgagt cgaagaactc ggcgttcgac ttggtggctt tcagcttgtc gatgaggaat
     1081 tcggcggcct cgagctcgtc catcgggaaa agaagcttcc tcagcaccca caccttctgc
     1141 aggatcggct gctcgatgag cagctcctcg cggcgcgtgc ccgagcggtt gacgttgatc
     1201 gccgggtaga tgcgcttctc gtgcatgcgg cggtcgaggt ggatctccat gttgccggtg
     1261 cccttgaact cctcgtagat cacgtcgtcc atgcggctgc cggtgtcgat cagcgcggtg
     1321 gcgatgatgg tgagcgagcc gccttcctcg acgtggcgcg cggcgccgaa gaagcgcttc
     1381 ggcttctgca gcgcgttggc gtcgacgccg ccggtcaata ccttgcccga ggccggcacc
     1441 accgtgttgt aggcgcgcgc caggcgggtg atggagtcga gcaggatcac cacgtccttc
     1501 ttgtgctcga ccagccgctt cgccttctcg atcaccatct cggcgacctg cacgtggcgc
     1561 gaggccggct cgtcgaaggt cgaggccacc acctcgccgc gcaccgagcg cgacatctcg
     1621 gtgacttcct cgggccgctc gtcgatcagc agcacgatca gcaccacttc gggatggttg
     1681 gcgacgatgg cgtgcgcgat gtgctgcagc atcaccgtct tgccggcctt cggcgggctg
     1741 acgatgaggc cgcgctggcc cttgccgatc ggcgccacga tgtcgatgac gcgcccggtg
     1801 atgttctcct cggccttgat gtcgcgctcg agcttgaacg cctcgtccgg gtgcagcggc
     1861 gtcaggttct cgaacaggat cttgttcttc gccgcctcgg ggacttcgcc gttgaccttg
     1921 tcgaccttga ccagcgcgaa gtagcgctcg ccgtctttgg gggtgcgaat ttccccttcg
     1981 atggaatcgc cggtatgcag gttgaagcgc cggatctgcg agggcgagac gtagatgtcg
     2041 tcggtgctcg ccaggtagga ggtctcgggc gagcgcagga agccgaagcc gtccggcagc
     2101 acctctaatg tgccgtcgcc aaatatggat tcgcccttct tcgcctggtt cttgagcagc
     2161 gcgaagatca ggtcctgctt gcgcaggcgg ttcgcgccgt cgatgccgtt cgacaccgcc
     2221 atctcgacca gctgcgtgac gtggtgcgac ttcagctcgg agaggtgcat gggaatggga
     2281 atggggacag atgcgggccc gcccggcgac ggcggcgtcg actcgggacc ttgggatgaa
     2341 cggacttctt ccggcggaac ggcctggggc ctgctcattc tcggggtgtg cgccggggaa
     2401 ttcttcggcg ctggaacctg ctgccgcgcc ggacatccgg cgcggctttc aaaagagggt
     2461 aaggggtttg gatattgctg tcaaatattg ctgtccagga aggcggtgag ctgggacttc
     2521 gagagcgcgc cgacgcgcgt cgcctcgacg ttgccgttct tgaacagcat cagcgtcggg
     2581 atgccgcgga tgccgaactt cgccggcacc gcctggttct cgtcgacgtt gatcttggcg
     2641 atcttgaggc gcccctcgta ctcccttgcc agctcgtcga ggatcggcgc gatcgacttg
     2701 cacgggccgc accattcggc ccagtagtcg accagcaccg gggtctcgga cttgaggacc
     2761 tcgggatcaa aggtgtcatc ggtcacatga cggatattgg cgctgctcat gactcctctg
     2821 ggggtgttgc aggaaggcct ctattatgag gctcccgccc cggaaatgct aaactgttga
     2881 ttcgccgaac cttatcatcc gccctatgac ctacgtagtg accgagtcct gcatcaagtg
     2941 caagtacacc gactgcgtcg acgtgtgccc ggtcgattgc ttccgcgagg ggccgaacat
     3001 gctggtgatc gaccccgacg agtgcatcga ctgcacgctg tgcgtccccg agtgcccggt
     3061 cgaggcgatc ttcgccgagg acgacgtgcc cgacgcgcag aaggaattca ccgcgcttaa
     3121 cgccgagctc tcgcgcctgt ggaagccgat catcgagaga aagcccgcgc cgcccgatgc
     3181 ggacgactgg gcaaagacca aggaaaaaaa acacctgctc gagcgctgag ctgtcgcccg
     3241 acggcgacgt ttctctctgc gttttttttg cgcactgaaa cacttttgaa aggtcgaagg
     3301 cgtaacctgt ctgctggctc acgcaggaga cgagaatgga acaagcaagg cttgcgttca
     3361 ccgcggtata cctgaagtcg aactcgggct acatcggctt catcgaggag ctgccgggag
     3421 tgaactcgca tggccgcacg ctcgacgaag cgcgcgccac gctgcagaag ctggcggcgg
     3481 tggtattcga cgaagagcgc cgcgaggcgg aagagctgat cgccggcaag gacgtggtgc
     3541 gcgaatcctt ttttgttccc attccgcgcc cttagcgcac tgcagcatat gcattgagtt
     3601 tttgggtctg gactcccggc cgccgccgcg gcgtaatatt cggcaacacg ggagggacag
     3661 cgcatggctg cgaaaagctg gcgtacggaa gactgggtcg cggtctacct ggggttcttc
     3721 gtcatcgccg tggttctcgc ggcgttcagc tggaaatggt tcgatctcgg cgtcgccagc
     3781 tcgacgttcc gctggaccgc ggacgcgcag atcgcgggct cgacgcccgg ctggcgcgcc
     3841 gcgctcgacg acgtcgcgaa ggaggcccgc gccaagggca ggaaggagct cgccgccagg
     3901 gcgggcgggc tcaaggcggc gctcgacaag ggcgagcgca aggggatcga gaaggcggcc
     3961 ggcgacctgt ccaaggccgg cggccgcaat accgtggcgg gcacgctcgg ctccgagatg
     4021 cgcgggcacg tggcggcgac ggcggacaag gtcttctccg cgggcaacct gggcaaggtg
     4081 ctcaccgtcg gcatcgcgct gctggtcatc ggggcagccg gatttgcgct catcggcggc
     4141 aatgtcggcg cgttcctcat aggtttcccg gcggtgttcg tcctcgcctg gctggcgcgg
     4201 gtcatcgccg gcaacggcct gttcatcgac tacggcgtgg agtacgtgat tttcgcgctg
     4261 gtcatgggat tgctggtgag caacaccatc ggcacgccga aatggctgcg ggccgcggtg
     4321 cagaccgagt atttcatcaa gaccggcctg gtgatcctgg gcgccgggct gctcttcctg
     4381 gaggtgatcc aggcaggcgt gctcggcatc ctgcaggcag tactggtggt gttcgtgatc
     4441 tggtacgtct gcttctggtt cgcgcgcaag ctgcgggtgg acg
//
LOCUS       DASPXW010000229         2599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1455417, whole genome shotgun sequence.
ACCESSION   DASPXW010000229 DASPXW010000000
VERSION     DASPXW010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455417"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="VEL04_05380"
     CDS             <1..562
                     /locus_tag="VEL04_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47830.1"
                     /translation="GAGSTVSIYPTVMNNVFNTKFKIIMGYKGSAEAQLAVERGEVEG
                     HSTAWTAVKVAHPDWRPSKKISILAQFALTPHPDLKDVPTAVSLARNDEERQILSAIV
                     AAADIGLAFFTTPGVPADRLAALRRAFDATMKDKDFLDEVEKLKLTASPMKGEDLQQL
                     IKQVSSLSPAMLEKVRTAYTANQAKE"
     gene            802..2367
                     /locus_tag="VEL04_05385"
     CDS             802..2367
                     /locus_tag="VEL04_05385"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007733837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HYT47831.1"
                     /translation="MGLDQLASKATSKTAARIDYDTYRLRSFVEALGEAELERRSGTT
                     KLAAIAGTLEANPKAVLFEAAGSGGYPLVGNALASRSRFALAFGVTPQKLLPEILRRL
                     RSKPEIVEVSREEAPVQAVVLTGADIDLTKLPVHLQHGKDGGLYISAGMDFARDPATG
                     QYNVGLRRFMLHGRASTGIDLVAPSDLRTIYQAAIARGERLPLSVVVGGHPVDYFAAT
                     MRMPGDELSLLASLRAQPMPIVKCVSNDLTVPADAEWVLEGYLGEEGYQEPEGPYGEF
                     LGYYGGVKTNPIFHVTAVTRRRDALLQTLTIGGASMSRTDTAQLTTLRTEVLIWRALE
                     TAIREPVAVYAPPQTGGIYNVRIAMRQRVPGEARNAIAAAFGSLANVKNVFVVDPDID
                     IFSDEQMEWALATRFQPDRDYVVASAFRTIPLDPSLASHGSLGAKAGFDLTMPIGAQK
                     KLETQIPEPPHFEGARFASVEDALRDGPKFFEQLMTAVGSEDGREIVLELEDLRSAGR
                     LERDPNDGRYRLK"
BASE COUNT          487 a          871 c          828 g          413 t
ORIGIN      
        1 gggtgccggc tcgaccgtct cgatctatcc gaccgtcatg aacaacgtgt tcaacaccaa
       61 gttcaagatc atcatgggct acaagggttc ggccgaagcg cagctcgcgg tcgagcgcgg
      121 cgaggtcgaa gggcactcga ccgcgtggac tgcggtgaag gtcgcccacc cggattggcg
      181 gccaagcaag aagatctcga tcctggcgca gttcgcgctg acgccgcatc ccgatctcaa
      241 agacgtcccc accgccgtca gcctcgcgcg caacgacgag gagcgccaga tcctgagcgc
      301 gatcgtggcc gcagccgata tcgggttggc attcttcacg acaccgggcg tgccggccga
      361 ccgcctcgct gcgctacgcc gcgcctttga cgccacgatg aaggacaagg acttcctcga
      421 tgaggtcgag aagttgaagc tcaccgcttc gccgatgaag ggcgaagacc tgcagcagct
      481 tatcaagcag gtcagctccc tgtcgcccgc catgctggag aaggtccgca ccgcctatac
      541 cgccaaccag gcaaaggaat agcgcgtgaa actgtgcctc tccagggtca gcgaggggca
      601 taggacgggc ataggataag ccggctgaag ctcgctgagc gagctcccgg ccagaaactc
      661 cgctcattcc cgcgcttccc cgccttcgcg aggacaggcg cgggaatccc cgttttcgcg
      721 gggacgaacg gagactcgtt ttcatcggaa atgctactgt cgcgccaagc atttcttaag
      781 agaacgagac aaggataacc catggggctc gaccagctcg cgagcaaagc aacaagcaag
      841 acagccgctc gcatcgatta cgacacctat cggctgcgca gcttcgtcga agcgctcggc
      901 gaagctgagc tcgagcgccg gagcggcacc accaagctcg ccgcgatcgc cggcacgctc
      961 gaagccaatc cgaaggccgt gctgttcgag gcggccggct cgggcggcta tccgctggtc
     1021 ggcaacgcgc tcgcgagccg ctcgcgcttc gctttagcct ttggcgtgac gccgcagaag
     1081 ctgctgcccg agatcctgcg gcggctgcgc agcaagccgg agatcgtcga ggtcagccgc
     1141 gaggaagcgc cggtgcaggc ggtggtgttg accggcgccg acatcgacct caccaagctc
     1201 ccggtccatc tccagcatgg caaggacggc ggtctctaca tttccgccgg catggacttc
     1261 gcgcgcgatc cggcgaccgg gcagtacaat gtcggcttgc ggcgcttcat gctgcatggc
     1321 cgcgcctcga ccggcattga ccttgtggcg ccaagcgacc tgcgcaccat ctatcaggcg
     1381 gcgatcgcgc gcggcgagcg acttccgctg agcgtggtgg tcggcggcca tccggtcgac
     1441 tatttcgccg ctaccatgcg catgccgggc gacgagcttt ccctgctcgc ttcgctgcgc
     1501 gcgcagccga tgccgatcgt caaatgcgtg agcaacgatc tgaccgtgcc ggccgacgcc
     1561 gaatgggtgc tcgaaggcta tctcggcgag gaaggctatc aggagccgga agggccttac
     1621 ggcgagttcc tcggctacta cggcggcgtc aagaccaatc caatcttcca tgtgacggcc
     1681 gtaacacggc gccgcgacgc gctgctgcag acgctcacca tcggcggcgc cagcatgagc
     1741 cgcaccgaca ccgcgcagct cacgacactg cgcaccgagg tgctgatctg gcgtgcgctc
     1801 gagactgcga tccgcgagcc ggtcgcggtc tacgcgccac cgcagactgg cggcatctac
     1861 aacgtgcgca tcgccatgcg ccagcgcgtg ccgggcgaag cgcgtaacgc cattgcggcc
     1921 gcgttcggct cgctcgccaa cgtcaagaac gtgttcgtgg tcgatcccga catcgacatc
     1981 ttttccgacg agcagatgga gtgggcgctc gccacgcgct tccagcccga tcgcgactac
     2041 gtggtcgcga gcgcgttccg caccatcccg ctcgatccgt cgctcgccag ccacggcagt
     2101 ctcggcgcca aggccggctt cgatctcacc atgccgatcg gtgcgcagaa gaagcttgag
     2161 acgcagattc ccgagccgcc gcatttcgaa ggcgcgcgct ttgcatcagt cgaggacgcg
     2221 ctgcgcgacg ggccgaagtt cttcgagcag ctgatgaccg cggtcggcag cgaggacggc
     2281 cgcgagatcg tgctcgagct cgaggacctg cgcagcgccg gccgcctcga gcgcgacccg
     2341 aacgatggcc gctaccggct gaaatgatgc agaaaaccgt catcctgacg tggccgcacg
     2401 gcctcatggt gaggaggcgc gcgcagcgcg ccgtctcgaa ccatggcagc aggctcggac
     2461 tcgcggccat ccttcgagac gccctcgctt cgctcgggct cctcgggatg aggtcgaaat
     2521 ggtggaaaga ttcacagcct ctgagggtga cggaccaaac ttggagaccc aggaaagaaa
     2581 ctgcggctcc catgcattc
//
LOCUS       DASPXW010000230         2126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_948502, whole genome shotgun sequence.
ACCESSION   DASPXW010000230 DASPXW010000000
VERSION     DASPXW010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948502"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            241..702
                     /gene="lptE"
                     /locus_tag="VEL04_05390"
     CDS             241..702
                     /gene="lptE"
                     /locus_tag="VEL04_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HYT47832.1"
                     /translation="MTAGCGFQLRGQAQLPFDTLYVPGGSPLAVELKRNVAAASRTRL
                     VDSAGDAQAVLGFTVELRDKVILSFDSAGRVSEYKLRYRVGFRVTDPKGGQVYLPTSE
                     VLLTRDMSYSDAQVLAKEAEEALLYRDMQSDMVQQILRRLVAAKPVQPRPE"
     gene            717..1724
                     /gene="holA"
                     /locus_tag="VEL04_05395"
     CDS             717..1724
                     /gene="holA"
                     /locus_tag="VEL04_05395"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HYT47833.1"
                     /translation="MTALRSEQLEAHLARELRPLYLIHGDEPLLALEAADAIRARARA
                     NGFSERLVLAAERGFDWNELGAGGASPSLFGDRKLIELRLASGKPGSDGAEAIEAFCA
                     KLPPDALTLVVLPRLDKAGQSSPWFKALEAKGVVVNIYPVERARLPEWIAARLARQKQ
                     SAARDTLQFLSDCVEGNLLAAHQEIQKLALLLPAGELDFDAVREAVMNVARYDVLKLT
                     DAMLSGERARLARMLEGLRGEGEAPPRVLWILAEEIRAIARVQKGLAAGRPLTEVCRE
                     ARVWGDARQTLVGRAAKKLPRAALLSALEHAASVDRVIKGVVKGDAWDELLQLGLRFA
                     A"
     gene            1838..>2126
                     /locus_tag="VEL04_05400"
     CDS             1838..>2126
                     /locus_tag="VEL04_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47834.1"
                     /translation="MAHTFPELKKKPLTELREIAAGIEHEAVKGYTQLNKDHLLAALC
                     KALNIDMHVHHEVKGVDKTAIKTKIREWQKKRDEALSAKDRAKLKVALNHIH"
BASE COUNT          372 a          710 c          729 g          315 t
ORIGIN      
        1 cggccggccg cgacgagctc gagcagctcg cgctcgccca cgacgccgtc acccgctaca
       61 ccaacggcca ggcggttaag aaggtcgtcg tggttcccgg acggctggtc aatgtggtcg
      121 tctgatcgca agccgctggg tccccgctcc ccgattaagg cattcgggga caggtttcgc
      181 ggggacgacg gtcactcccg ccgccttcaa gcgctcgttt cgctcgcgct tgcggcgctg
      241 gtgaccgccg gctgcggttt ccagctccgc ggccaggcgc agctgccttt cgacacgctc
      301 tacgtcccgg gaggctcgcc gctcgcggtc gagttgaagc gcaacgtcgc cgcggcaagc
      361 aggacgcgcc tcgtcgacag cgccggcgac gcgcaggccg tcctcggctt caccgtggag
      421 ctgcgcgaca aggtcatcct ctcgttcgac agcgcgggcc gcgtgagcga atataagttg
      481 cgctatcgcg tcggattccg cgtcaccgat cccaagggcg gccaggtgta cctgccgacc
      541 agcgaagtcc ttctgactcg cgacatgtcc tacagcgacg cgcaggtgct cgccaaggag
      601 gccgaggaag cgctgctcta ccgggacatg cagagcgaca tggtgcagca gatcctgcgg
      661 cgcctggtcg cggcgaagcc ggtgcagccc aggcccgagt agggacaggc agcgcgatga
      721 ccgccctccg gtccgagcag ctcgaagcgc atctcgcgcg ggagctccgg ccgctctacc
      781 tgatccacgg cgacgagccg ctgctcgcgc tcgaggccgc cgatgcgatc cgcgcgcggg
      841 cgcgcgcaaa cgggttttcc gagcgcctgg tgctcgccgc ggaacgcggc ttcgactgga
      901 acgagctcgg cgcgggcggc gcgagccctt cgctcttcgg cgacaggaag ctgatcgagc
      961 tgcgcctcgc ctcaggcaag cccggcagcg acggcgccga ggcgatcgaa gcgttctgcg
     1021 cgaagctccc gcccgacgcg ctcactctcg tggtgctgcc gcggctggac aaggcgggcc
     1081 agtcgtctcc gtggttcaag gcgctcgaag cgaagggcgt cgtcgtcaac atctatccgg
     1141 tcgagcgcgc gcggctcccc gagtggatcg cggcccggct cgcacggcag aagcagagcg
     1201 cagcgcggga taccctgcag tttctctccg actgcgtcga aggaaacctt cttgcagcgc
     1261 accaggagat ccagaagctc gcgctgctct tgcccgctgg ggagctggac ttcgacgccg
     1321 ttcgcgaagc ggtgatgaac gtggctcgtt acgacgtact caagctcacc gacgcgatgc
     1381 tctcggggga gcgcgcgcgg cttgcgcgca tgctcgaagg cctgcgcggc gaaggcgaag
     1441 cgccgccgcg cgtgctgtgg atcctcgccg aggaaatacg cgccatcgcc cgagtgcaga
     1501 aaggtctggc ggcgggcagg ccgctcacgg aagtatgccg cgaggcgcgc gtgtggggcg
     1561 atgcgaggca gacgctggtg ggcagggccg cgaagaaact cccgcgtgca gcgctgctct
     1621 ctgccctgga gcacgcggcg agcgtggacc gcgtcatcaa gggcgtggtc aaaggcgacg
     1681 cgtgggatga attgctgcag cttgggcttc gcttcgcggc ctgatttcgc gtcgccgccg
     1741 aagctgctgc acgatatgaa agcgcgcgcg ggtacgcctc tcacggtgta agattcgccg
     1801 aggtttccgg aaaacagcca acgtacgagg agacagcatg gcgcatacct tcccagagct
     1861 caagaaaaaa cccctcaccg agctgcgcga gatcgccgcc ggcatcgagc acgaggcggt
     1921 gaagggctac acgcagctga acaaagacca tctgctcgct gcgctctgca aggcgctcaa
     1981 catcgacatg cacgtgcacc acgaggtgaa gggcgtcgac aagaccgcga tcaagacgaa
     2041 gatccgcgag tggcagaaaa agcgcgacga ggcgctctcg gcgaaggacc gggcgaagct
     2101 caaggtcgcg ctcaaccaca tccacc
//
LOCUS       DASPXW010000231         3505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_319564, whole genome shotgun sequence.
ACCESSION   DASPXW010000231 DASPXW010000000
VERSION     DASPXW010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319564"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="VEL04_05405"
     CDS             <1..817
                     /locus_tag="VEL04_05405"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HYT47835.1"
                     /translation="LDAVNVVDPDCAVLTSVGIDHIDYLGADRESIGREKAGIFRSAK
                     PAVIAEPDPPQSVLAAQGVKLVIKKDFGYEAVEGQWTYWGPGGRRAGLAYPALRGAVQ
                     LRNAAAALCALEALKLPIAMQEIRRGLAEVALAGRFQVLPGRPQVVLDVAHNPQAAQT
                     LAENLGASGFAAETIAVCGMLRDKDIGGVLRAMAPRITRWHLATLPGPRGARAEELAK
                     HLDVDDVRLHASPAAAFEAAAESAEENDKIVVFGSFLTVGEVMAWLKRTTSKH"
     gene            787..1275
                     /locus_tag="VEL04_05410"
     CDS             787..1275
                     /locus_tag="VEL04_05410"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HYT47836.1"
                     /translation="MAEAHDVETLKRRGRRRLVGAVALVLAAVIILPMVFDPEPRRAD
                     APVSVRIPGENETPFAPKVAPKAREKKIEAKKAEVKKAEAKKTEASAEYVVPVGAFAN
                     PDGVIAKLAAARLPYYTEAVKGNLTRVRAGPFASREAAEKALQQLKGMGLAPGTVTTK
                     AG"
     gene            1272..1763
                     /locus_tag="VEL04_05415"
     CDS             1272..1763
                     /locus_tag="VEL04_05415"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HYT47837.1"
                     /translation="MTWLDYTLIGVLLVSIAWGVWRGLVREAISLAGWVVAFLVANLF
                     AAPLVELLPKSLPRPEWHLPLAFVVIFLLTLTLATLVGLLLSKLVTKVGLGGLDRTLG
                     GLFGLVRGWFIALALALLAGLTVLPLHPIWKESLSGPTLARAAIYLKDWLPPAFADRL
                     RYH"
     gene            1793..3310
                     /gene="purF"
                     /locus_tag="VEL04_05420"
     CDS             1793..3310
                     /gene="purF"
                     /locus_tag="VEL04_05420"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HYT47838.1"
                     /translation="MCGILGIVARSPVNQLLYDGLLLLQHRGQDAAGIVTSERNTFHM
                     HKASGMVSEVFRTRNMRALPGNIGIAHCRYPTAGSARNSAEAQPFYVNSPFGIVLGHN
                     GNLTNADQLKEEMFRTDLRHINTGSDSEVLVNVLAHELEQASVKLRLEPQTIFTAVTN
                     VHRRCKGAYAVVAMIAGSGVLAFRDPFGIRPAVIGYNETEAGTEYMVASESVAVDALG
                     FRVLRDIGPGEAIFIDDDGNFHSQQCAPQASLNPCIFEYVYLARPDSLIDGASVYETR
                     LNMGEKLGEKIRRQYRHLDVDVVIPIPDSSRPSALQLAAGLGVPYREGFVKNRYIGRT
                     FIMPGQAVRARSVRQKLNPMSMEFSGKNVLLVDDSIVRGTTSREIVQMARESGARKVF
                     FASAAPPVRFPNVYGIDMPTRAELIAAHRSEEEVAREIGADALVYQDLEALKDAVRRA
                     NPRLASFETSCFDGIYVTGDVTIDYLRAIEIRRDATRDSGEDEGAQLDLNLATAR"
BASE COUNT          607 a         1133 c         1189 g          576 t
ORIGIN      
        1 gctcgacgcg gtaaacgtgg tcgatcccga ttgcgcggtg ctgaccagcg tcggcatcga
       61 ccacatcgat tatctcggcg cggaccgcga gtcgatcggc cgggagaaag ccggcatctt
      121 ccgttccgca aaacccgcag tgattgccga gcccgatccg ccgcaatccg tattggcggc
      181 gcaaggcgtg aagctcgtta taaaaaaaga ctttggttat gaagcagtgg aggggcagtg
      241 gacctactgg ggtccgggag ggcggcgcgc cgggctcgcc tatccagcgc tccggggagc
      301 ggtgcagctg cgcaatgcgg cagccgcgct gtgcgcgctg gaggcgttga agctgccgat
      361 cgcgatgcag gagattcgcc gcgggcttgc ggaggtcgcg ctcgcgggcc gcttccaggt
      421 gctccccgga cgccctcagg tcgtgctcga tgttgcgcac aacccgcagg cggcgcagac
      481 gctggccgag aatctcggcg cctccggatt cgccgccgag accatcgcgg tgtgcggcat
      541 gctgcgcgac aaggatatcg gcggggtgct gcgcgcgatg gcgccgcgca tcacgcgctg
      601 gcatctcgcg acgctgcccg ggccgcgcgg ggcgcgcgcc gaagagctgg cgaagcatct
      661 cgatgttgac gatgtgaggc tgcacgcatc gccggccgcg gcgttcgaag cggcggcgga
      721 gagcgcggaa gagaatgata agatcgtcgt gttcggttcc tttcttacgg ttggcgaagt
      781 gatggcatgg ctgaagcgca cgacgtcgaa acactgaagc gccgcggacg caggcgcctg
      841 gtcggcgcgg tggcgctggt gctcgctgcg gtcatcatcc tgccgatggt gttcgacccg
      901 gagccgcggc gcgccgatgc gcccgtgagc gtgcgcatac ccggcgagaa cgagacgcct
      961 ttcgcgccca aagtggcgcc gaaggcccgc gagaagaaaa tcgaagcaaa gaaagccgag
     1021 gtaaagaaag ccgaggcaaa gaaaaccgag gcgagcgccg aatacgtcgt gccggtgggg
     1081 gcgtttgcca atcccgacgg cgtgatcgcg aagctcgccg cggccaggct gccctactac
     1141 acggaagcgg tgaaaggtaa cctgacgcgc gtgcgcgcag ggccctttgc gagcagggag
     1201 gctgccgaga aggcgctgca gcagctgaag ggcatgggcc tcgcaccagg cactgtgacg
     1261 acgaaggccg gatgacctgg ctcgactaca ccctcattgg cgtgctcttg gtatcgatcg
     1321 catggggcgt atggcgcggc ctggtgcgcg aggcgatctc gctcgccggc tgggtggtcg
     1381 cctttctcgt cgcgaacctg ttcgccgcgc cgctcgtgga gctgctgccg aaatcgctgc
     1441 cgcggcccga atggcacctg ccgctcgcgt tcgtggtgat tttcctgctg acgctgacgc
     1501 tcgccacgct ggtgggcttg ctgctctcga agctggtcac gaaggtcggg ctcggcgggc
     1561 tggaccgcac gctcggcggg ttgttcggct tggtgcgcgg ctggttcatt gcgctcgcgt
     1621 tggcgctgct agcgggcttg actgtgctgc ctttgcaccc catatggaag gaatccctta
     1681 gcggtcccac gctggcgagg gcggctatct acctcaagga ctggctgccg cccgcattcg
     1741 ccgaccgact gcgatatcat tagccgttaa ccacacccgg aattagcgtt ccatgtgcgg
     1801 gatcctggga atcgtcgcgc gctcgcccgt caaccagctg ctctacgacg ggctgctcct
     1861 gctgcagcac cgcggccagg acgcggccgg catcgtcacc agcgagcgca acacctttca
     1921 catgcacaag gcgagcggca tggtgagcga ggtcttccgc acccgcaaca tgcgcgcgct
     1981 gcccggcaat atcggcatcg cgcactgccg ctatccgacc gcaggctcgg cgcgcaattc
     2041 ggccgaagca cagcccttct acgtcaactc gccgttcggc atcgtgctcg gccacaacgg
     2101 caacctgacc aacgccgacc agctcaagga agagatgttc cgcaccgact tgcggcacat
     2161 caacaccggc tcggactcgg aagtactggt gaacgtgctc gcgcacgagc tggagcaggc
     2221 ctcagtgaag ctgcgcctcg agccgcagac gatcttcacc gccgtgacga acgtgcaccg
     2281 gcgctgcaag ggagcctatg ccgtggtggc gatgatcgcg ggctccggtg tgctcgcgtt
     2341 ccgcgacccc ttcggcatcc gtcccgccgt catcggctac aacgagaccg aggccggcac
     2401 cgagtacatg gtggcctccg agtcggtcgc ggtggatgcg ctcggctttc gcgtgctgcg
     2461 cgacatcggt ccgggcgagg cgatcttcat cgacgacgac ggcaatttcc acagccagca
     2521 atgtgcgccg caggcgagcc tcaacccctg catcttcgaa tacgtctacc tggcgcgccc
     2581 ggactcgctg atcgacggcg cctcggtcta cgagacgcgc ctaaacatgg gtgagaagct
     2641 cggcgagaag atccggcggc agtaccgcca cctcgacgtc gacgtggtga tcccgatccc
     2701 ggactcgagc cggccctcgg cgctgcagct cgctgccggg ctgggcgtgc cctaccgcga
     2761 gggcttcgtg aagaaccgct atatcgggcg caccttcatc atgcccggcc aggcggtgcg
     2821 cgcgcgctcg gtgcgccaga agctcaaccc catgtcgatg gagttcagcg gcaagaacgt
     2881 gctgctggtg gacgactcga tagtcagggg cacgaccagc cgcgagatcg tgcaaatggc
     2941 gcgcgagtcg ggcgcgcgca aggtgttctt tgcctccgcc gcgccgccgg tgcgcttccc
     3001 gaacgtgtac ggcatcgaca tgccgacccg cgccgagctg atcgccgcgc accgcagcga
     3061 ggaagaggtg gcgcgcgaga tcggtgctga cgcgctcgtc taccaggacc tggaagcact
     3121 caaggacgcg gtgcggcgcg ccaacccgcg cctcgccagc ttcgagacct cgtgcttcga
     3181 cggtatctac gtcaccggcg acgtcaccat cgactacctg cgcgcgatcg agatccggcg
     3241 cgacgcgacc cgcgactcgg gcgaggacga aggcgcccag ctcgacctca acctggcaac
     3301 cgcgaggtaa gtttggtata gtccgttttt cgcgccgatt tgagccacag cttgcgggcg
     3361 cattacaaat tcagctaaag cgtgccgaga gaacccggtc cgctagcaaa gagaggccgg
     3421 gttttttatg ggcaagaagc cagagttagc aacgctgggc gtgcgcgccg gtcaggtgcg
     3481 cagccagttc caggagcatt ccgag
//
LOCUS       DASPXW010000232         6120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1332390, whole genome shotgun sequence.
ACCESSION   DASPXW010000232 DASPXW010000000
VERSION     DASPXW010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6120
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332390"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1034
                     /locus_tag="VEL04_05425"
     CDS             <1..1034
                     /locus_tag="VEL04_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYT47839.1"
                     /translation="LAVEFMVMAVVLVLRPWGLLGRPSVPARGPASVEPPLGALSSRG
                     ALTLAALALALAAFPLAADDYSVVLLTDVLVFALFAVSLHFIMGPGGMLSFGHAAYFG
                     LGAYGAGLALLRLGLPMEFALACAPLAALAGAVAFGWFCVRLSGVYLAMLTLAFAQIV
                     WSLAFQWDALTGGSNGLVGIWPAAWLSSRPAYYYLTLVLCLAGIALLWRMLYSPFGYA
                     LRAVRDSPLRADAIGIDLRGIQWRGFVVAGVFSGLAGGLYAFSKGSISPETLSMPRSV
                     DGLVMVLLGGVQTLTGPAWGAALLIWLEDSLSRGFEYWRGVMGAVILLLALAFPQGLA
                     GFLKERFGR"
     gene            1031..1768
                     /locus_tag="VEL04_05430"
     CDS             1031..1768
                     /locus_tag="VEL04_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016355145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT47840.1"
                     /translation="MRLAVRNLSKAYGGVQALRGISFSVAAGELVALIGPNGAGKTTC
                     FNLLNGQLAPDAGEILLDGRSLAGLRPHHVWRLGVGRTFQVTATFGSMSVRENVQLAL
                     LSHHGRLHSFWSVASKLEILPADALLAQVGMLDQAARPCAVLAYGDLKRVELAIALAN
                     RPRLLLMDEPTAGMAPAERGALMELASGLAGSEGIAVLFTEHDMDVVFNYASRVVVLD
                     SGEVIAAGAPAEVRADARVRQLYLDDA"
     gene            1761..2417
                     /locus_tag="VEL04_05435"
     CDS             1761..2417
                     /locus_tag="VEL04_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT47841.1"
                     /translation="MLELRRLDAGYGRAQVLFGVSLELRPGEVVVLLGRNGAGKSTTL
                     KAIMGLVRAVSGEVCWRGRRIERLEPFEIARLGLGYVPEDRRIFTDLSVAENLEVGRQ
                     APRRGLPAWTAEKLFELFPNLAATAQHPAARISGGEQKMLAIARTLMGNPAAILLDEP
                     SEGIAPVLARQIAETIVGLKRAGMAVLLCEQNARLARRVADRGYLIENGELREQALPV
                     "
     gene            2414..2905
                     /locus_tag="VEL04_05440"
     CDS             2414..2905
                     /locus_tag="VEL04_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47842.1"
                     /translation="MTRRGLLLTMTEPPAAMEEEFNAWYDTEHMAERLAIPGFRSARR
                     WVADVAPGEGKYLATYELDSLKILHSPAYLARFQNQTPWSRRCLGKTAVFKRWVCEQI
                     DPGDADPHSTAQALLFTFQSVPPGLSGVLQTRRFRACGDPRDIVLCELAQPVPGSYSA
                     YGT"
     gene            2895..3788
                     /locus_tag="VEL04_05445"
     CDS             2895..3788
                     /locus_tag="VEL04_05445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HYT47843.1"
                     /translation="MVLERFALLSVAAAVATIALKTLAWWLTGSVGLLSDALESLVNL
                     AAALLALSMLRLAATPPDEAHPYGFSKAEYLSAGIEGALIVLAAAGILFTAIPRLIAP
                     QPLETPALGLGLSVAASLINLVVARILIKAGHEHHSITLEADGRHLMTDVWTSAGVIL
                     GVGLVFVTGWLQLDALVALAVAAHIVWTGIGLVRRSVSGLLDAAIPRDDQREVTKLFA
                     EYSKRYGVTFHALRTRQAGARRFVSFHLLVPDAWSVAQAHRLSEEIEARMRSMVPNAS
                     IITHIEPISDPASYEDQELDR"
     gene            complement(3820..4050)
                     /locus_tag="VEL04_05450"
     CDS             complement(3820..4050)
                     /locus_tag="VEL04_05450"
                     /inference="COORDINATES: protein motif:HMM:NF044585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47844.1"
                     /translation="MEEKTRVTIFTATYRVKGYIDLLPGARITDFMAEAKDFIAVVNA
                     EVWELQPGGRQVASAAFINVSRDHIEIILPGH"
     gene            complement(4055..5617)
                     /locus_tag="VEL04_05455"
     CDS             complement(4055..5617)
                     /locus_tag="VEL04_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYT47845.1"
                     /translation="MRPGPGNYSALSPIVFLPRAAEIFPERVAVIDGERRITYREFFE
                     RARRLASALQRKGIRRGDVVSAMLPNLPPMLEAHYGVPMLGAVLNTINTRLDADSVAY
                     ILEHSGARALLTDRMFAAVVDPALKKLQRRILVIEVDAQYEAFLKTGNAEFAWSLPGA
                     ETDPIALNYTSGTTGRPKGVVYHHRGTFLEAAGNIMAWAMPARPVYLWTLPMFHCNGW
                     CFPWSVTAMGGTHVCLRAVEPKRIFELIVEHGVTHMCGAPTVLAMLIGASEADQRKFP
                     HPVHIQTGGSPPPAKVIKSMEELGFQVLHIYGMTELQGPSTFCAPQEAWKDFSLENRT
                     NQMARQGVRYPVVEGHIVADPKSCKPVPKDGKTVGEILVRGNTVMLGYLKDAKATAEA
                     FAGGWMHTGDLAVWHPTNYVEIKDRKKDIIISGGENISSVEVEIALYKHPATQLAAVV
                     ARPDEKWGETPCAFVQLKPGAAATEAEYIDWCRQNLARFKIPKKVVFGPVPTTATGKI
                     QKYVLRERAKAL"
     gene            5753..>6120
                     /locus_tag="VEL04_05460"
     CDS             5753..>6120
                     /locus_tag="VEL04_05460"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYT47846.1"
                     /translation="MKPIAIAAAAAYALVAGTAFAQEKTIKITGFGAKSGVVRSFGVN
                     SEAAMLAAADLINKQGGVTLADGSKAKFTVEFLDDRCSGEEGISVIRRIYGTEALVAV
                     GPTCSNVAEPLFGILQKKVD"
BASE COUNT          882 a         2047 c         2035 g         1156 t
ORIGIN      
        1 cgcttgctgt ggaattcatg gtgatggctg tggtgctggt gctgcgcccc tggggactgc
       61 tcgggcggcc gagcgtgccg gcgcgcggac cggccagcgt ggagccgccg ctgggcgccc
      121 tgtcttccag gggcgcgctt acccttgcgg cgctcgccct cgccctggcc gcgtttccgc
      181 tggctgcgga cgattacagc gtggtgctgc tcaccgacgt cctggtgttc gcgctgttcg
      241 cagtgagcct gcatttcatc atgggtcccg gcggcatgct ctcgttcggc cacgcggcct
      301 actttggcct cggtgcgtat ggcgccgggc ttgcgctgct gcgcctgggc ctgccgatgg
      361 agttcgcgct ggcgtgcgcg ccgctcgccg ccctcgcggg cgcagtggcg ttcggctggt
      421 tctgcgtgcg cctctcgggg gtctacctcg ccatgctgac tctcgccttc gcacagatcg
      481 tctggtcgct cgccttccag tgggatgcgc tcaccggcgg ctcgaatggg ctggtgggaa
      541 tctggccggc cgcctggctc tcgtcgcggc cggcgtacta ctacctgacg ctcgttctat
      601 gcctcgccgg gatcgcattg ctctggcgca tgctttactc gcccttcggc tacgccttgc
      661 gcgcggtgcg cgattcgccg ctgcgtgccg atgccatcgg catcgatctg cgggggatcc
      721 agtggcgcgg cttcgtcgta gccggggtgt tttccggcct ggccgggggc ctttatgcct
      781 tctcgaaggg atcgatctca cccgagacgc tgtcgatgcc ccgctcggtg gacggcctgg
      841 tgatggtgct gctcggcggc gtgcaaacgc tcaccggtcc ggcctggggc gccgcgctct
      901 taatctggct cgaagactcg ttgtcgcgcg gcttcgaata ctggcgcggc gtgatggggg
      961 cggtaattct cctcctggcg ctcgcattcc cgcagggcct cgccggcttc ctgaaggaaa
     1021 ggttcgggcg atgaggctcg cggtgcgaaa tctctcaaag gcctatggcg gcgtgcaggc
     1081 gctgcgtggc atatcgttct ccgtggctgc gggagaactc gtcgccttga tcggcccgaa
     1141 cggcgccggc aagacgacct gcttcaacct gctcaacggc cagctcgcgc cggatgccgg
     1201 cgagatcctg ttggacggcc gcagcctggc cggcctgcgg ccccaccatg tctggcggct
     1261 gggcgtggga cgcactttcc aggtcacggc gaccttcggc tcgatgagcg tgcgcgaaaa
     1321 cgtgcagctg gcgctgctgt cgcaccacgg ccgccttcac tccttctggt cggtcgcctc
     1381 gaagctcgaa atcttgcccg cggatgcact gcttgcgcag gtggggatgc tcgaccaggc
     1441 ggcgcgccct tgcgccgtgc tcgcttatgg cgatttgaag cgcgtggagc tggcaattgc
     1501 gcttgcgaac cggccgcgcc tcctgctgat ggacgagccg accgcgggca tggcgcccgc
     1561 cgagcgcggc gccctgatgg agctcgcatc gggcctcgca ggcagcgagg gcatcgcggt
     1621 actcttcacc gagcatgaca tggacgtcgt gttcaactat gcaagccgcg tcgtcgtgct
     1681 tgattcaggc gaggtgatcg ccgccggcgc gcccgccgaa gtgcgcgccg atgcccgcgt
     1741 gcggcaactc tacctcgacg atgcttgagc tccggcgcct cgacgcaggt tacgggcgcg
     1801 cgcaagtgct cttcggggtc agcctcgagc tgcgtcctgg cgaagtcgtg gtccttctcg
     1861 gacgcaatgg cgcgggaaaa tcgactaccc tgaaagccat catgggactg gtgcgcgcgg
     1921 tttcgggtga ggtgtgctgg cgcggccggc gcatcgagcg gctcgagccc ttcgagattg
     1981 cccgcctggg gctcggctac gtgccggagg accggcgcat cttcaccgat ctctcggtgg
     2041 cggaaaacct cgaggtcggc cggcaggcgc cgcgccgcgg cctgcccgcc tggacagcgg
     2101 agaagctgtt cgagctgttt ccgaatctcg ctgccacggc acagcatccg gcagcccgca
     2161 tctcgggcgg cgagcagaaa atgctcgcca tcgcccgcac actgatggga aatccggccg
     2221 cgatcctgct cgatgagccc tcggagggta ttgcgccggt gctcgcccgg cagatcgccg
     2281 agaccatcgt cgggctcaag cgcgcgggca tggcggtgct gctatgcgag cagaacgcgc
     2341 gcctcgcgcg ccgcgtcgcg gaccgcggct acctgatcga aaacggggag ctgcgggagc
     2401 aggcgttgcc ggtatgacgc gccgcgggct gctgctgacg atgaccgagc cgcccgcggc
     2461 catggaggaa gaattcaacg cctggtacga caccgagcac atggccgagc ggctggcgat
     2521 cccgggcttc cgctcggcgc gccgctgggt cgccgatgtc gcacccggcg aaggcaaata
     2581 cctcgcgacc tacgagctgg attccctgaa aatcctgcat tcgccagcct acctcgcccg
     2641 cttccagaac cagactccct ggagcaggcg ctgcctgggc aagacggcgg tgttcaagcg
     2701 ctgggtctgc gagcaaatcg atcccggcga tgccgacccg cattccactg cgcaggcttt
     2761 gcttttcacg ttccaatcgg ttcctcccgg cctcagcggc gtcctgcaga cgcggcgctt
     2821 tcgcgcctgc ggcgacccgc gcgacatcgt cctttgcgag cttgcacaac cggtcccggg
     2881 cagctatagc gcctatggta cttgagcgtt ttgccttgct gtcggtggcg gcggctgtcg
     2941 cgacgatcgc cctgaagacg ctcgcctggt ggctcacggg ctcggtcggc ctgctgtccg
     3001 acgcgctcga atccctggtc aacctggcgg ccgccctgct cgcgctgtcg atgctgcggc
     3061 ttgccgcgac gccgccggac gaggcgcatc cttacggctt ctcgaaagcc gaataccttt
     3121 ctgccggcat cgagggcgcg ctgatcgtgc tggcggcggc gggaatcctg ttcaccgcga
     3181 ttccacgtct gatcgcgccg cagcctctcg agacgcccgc gctcggcctg ggtttgagcg
     3241 tcgccgcctc gctgatcaac ctggtcgtgg cgcggatttt gatcaaggcc ggccacgagc
     3301 accacagcat caccttggaa gccgacggca ggcacctcat gaccgatgtc tggacatcgg
     3361 cgggcgtcat cctcggcgtc gggctggtgt tcgtcaccgg ctggctgcag ctcgatgcgc
     3421 tggtggcgct cgccgtcgcg gcgcatatcg tctggaccgg gatcggcctg gtgcggcgct
     3481 ccgtctccgg gctgctcgac gcggcgatcc cgcgcgacga ccagcgcgag gtgaccaagc
     3541 ttttcgccga atactcgaag cgctacggcg tgaccttcca tgcgctgcgc acgcgccagg
     3601 ccggcgcgcg ccgtttcgtc tcgttccatc tcctggtgcc cgacgcctgg agcgtcgccc
     3661 aggcgcatcg gctgtcggag gagatcgagg cgcgcatgcg ctcgatggtg cccaacgcct
     3721 cgatcatcac gcacatcgaa ccgatttccg acccggcctc ctacgaggat caggagctcg
     3781 accgctgaat ttaagcgggc tgcttcgcta cttttagggt caatgcccgg ggaggatgat
     3841 ctcgatgtga tcgcggctga cgttgatgaa agccgccgag gcgacctggc gcccgccagg
     3901 ctgcagctcc cagacttcgg cgttgaccac cgcgatgaaa tccttcgcct cggccatgaa
     3961 gtcggtaatc cgcgccccgg ggaggaggtc gatgtagccc ttgacccggt aggtcgcggt
     4021 gaaaatcgtg actctggttt tttcttccat gggttcagag cgcctttgcg cgttcgcgca
     4081 gcacgtattt ctggatcttg ccggtggccg tggtcggcac cggtccgaat actaccttct
     4141 tcggaatctt gaagcgcgcc aggttctggc ggcaccagtc gatgtactcg gcttccgtgg
     4201 cggccgcgcc cggcttgagc tgcacgaagg cgcacggcgt ctcgccccat ttctcgtccg
     4261 gccgcgctac caccgctgcg agctgcgtcg ccgggtgctt gtagagcgcg atctcgacct
     4321 cgaccgacga gatgttctcg ccgcccgaaa tgatgatgtc cttcttgcgg tccttgatct
     4381 cgacgtagtt cgttggatgc cacaccgcga gatcgccggt gtgcatccag cccccggcaa
     4441 acgcctcggc ggtggctttg gcgtccttga ggtagccgag catcacggtg ttgccgcgca
     4501 ccaggatttc gccgacggtc tttccgtcct tcggcaccgg cttgcagctc ttcgggtcgg
     4561 cgacgatgtg gccctccacc accgggtagc gcacgccctg ccgcgccatt tgattggttc
     4621 tgttttcgag agaaaaatcc ttccaggcct cttgcggggc acagaacgtc gacggtccct
     4681 gcagctcggt catgccgtag atgtgcagca cctggaagcc gagctcctcc atgctcttga
     4741 tcaccttggc gggaggcggc gagccaccgg tctggatgtg cactgggtgc gggaatttgc
     4801 gctggtccgc ctcggacgcg ccgatcagca tcgcgagcac ggtcggagcg ccgcacatgt
     4861 gcgtgacgcc gtgctcgacg atcagctcga agatgcgctt cggctccacg gcgcgcaggc
     4921 agacatgcgt gccgcccatg gcggtaaccg accacgggaa gcaccagccg ttgcaatgga
     4981 acatcggcag cgtccagagg tagaccggtc tcgccggcat cgcccaggcc atgatgttgc
     5041 ctgccgcctc gagaaaggtg ccgcggtggt gatacaccac gcccttcggc cggccggtcg
     5101 tgcccgaggt gtagttgagc gcgatcgggt cggtttccgc gcccggcagc gaccaggcga
     5161 actcggcgtt gccggttttc aggaaagctt cgtattgcgc atcgacctcg atgaccagga
     5221 tgcggcgctg cagttttttc aaggcaggat cgaccaccgc cgcgaacatc cggtcggtga
     5281 gaagcgccct cgccccgcta tgttccagga tgtaggcgac gctatcggcg tccagccgcg
     5341 tattgatggt gttgaggacc gcgcccagca tcggcacgcc gtaatgcgct tcgagcatcg
     5401 gcggcagatt cggcagcatc gccgagacga cgtcgccgcg gcgaatgcct ttccgctgca
     5461 gcgccgaggc aagccgccgg gcgcgttcga agaactcgcg gtaggtgatg cgccgctcgc
     5521 cgtcgatgac cgcgacgcgc tcgggaaaga tttcggcggc acgcggcagg aacacgatcg
     5581 gcgacaacgc gctgtaattc ccgggcccgg gacgcagggt tgcccaaggc gatttccgcg
     5641 ctgcaacaac acgccttcct gaagcgctgc cattcgactt tttcttgccg gtgtttccca
     5701 tcgccgcctc cccgctttac actgaacaat ctaccccagg gaggaggatc ccgtgaagcc
     5761 catcgccatt gctgcggccg ccgcatatgc gctcgtcgcc ggcaccgcgt tcgcgcagga
     5821 gaaaaccatc aagatcaccg gctttggcgc caagtcgggc gtggtgcgct cgttcggcgt
     5881 caacagcgag gcggcgatgc tcgccgccgc cgacctcatc aacaagcagg gcggcgtcac
     5941 gctcgcagat gggtcaaaag cgaaattcac ggtcgagttc ctcgacgacc gctgcagcgg
     6001 cgaagaaggc atctcggtga tccgccgcat ctacggcacc gaggcgctgg tcgccgtcgg
     6061 cccgacctgt tcgaacgtcg ccgagccgct gttcggcatc ctgcagaaga aggtcgacga
//
LOCUS       DASPXW010000233         2082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1078929, whole genome shotgun sequence.
ACCESSION   DASPXW010000233 DASPXW010000000
VERSION     DASPXW010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1078929"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2053)
                     /locus_tag="VEL04_05465"
     CDS             complement(<1..2053)
                     /locus_tag="VEL04_05465"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYT47847.1"
                     /translation="MDQARTIALSLVGSPAQESRGQYILGRIALKQKKPEEAVIAFAR
                     ATKLDARHGAAWGGLAEAYLALKDERKAREALASAAALPDATVAVFRQLADLEIRAAR
                     HAAALAPLERAVALLPGDADLRLAQARTFAALERWHDAANAAREAQRLAPKNVDALLL
                     GADAAYRQGKNGEAIETLKSAVALEPDSYDVQLKLARSYADNGLYVDAQSHLERAARL
                     NERSDAPHLMLAQMQLNQRSYDAAIATLTRAVKLNPSDANRRELEGAYDRKKKAQSGS
                     GGRIVMENLRLERVFVAAHKQYATEPLGRIKLRNDSAEDYKGLRLSFFIKEYMDFPVS
                     QEIPPLKSKGSLELTLNATFNNKVLGIDEDTRVLVVVTLALADSRDGSQEITQPMTLY
                     GKNALVWSNSDMIGSFVTPRDDTLRNFVREAANRYGGPPQSVLNRPLSQAATVFNVLS
                     ALGLRYQPDPNTPYSRLSADQVDYVQFPRETLKLKSGDCDDLSVLLAAAYENLGIESA
                     MVEVPGHLFMMFRTGVKDADRGLISLQDELLVIRDGEVWIPVEATLLAASFSEAWAEG
                     ARKYREAAAKKAVKVVSLRQAWERYPPATLAPAPLAIEVPSGDRVSRLIEREQNLLLA
                     RRLEREVLPYRQALAADPKDIDARLQIGTIYARNGVMDVAQREFDAVLALDPRNASAL
                     NN"
BASE COUNT          279 a          689 c          755 g          359 t
ORIGIN      
        1 gattgttgag cgccgatgcg ttgcgcgggt cgagcgcgag aaccgcgtcg aactcgcgct
       61 gcgcgacgtc catgaccccg ttgcgcgcgt agatggtgcc gatctgcagc cgggcgtcga
      121 tgtccttcgg gtcggcggcc agggcctgcc ggtaaggcag cacctcgcgc tcgagccggc
      181 gcgcgagcag caggttctgc tcgcgctcga tgagacggct cacgcggtct ccggaaggca
      241 cctcgatcgc gagcggcgcg ggagcgagcg tggcgggcgg atagcgctcc caggcctggc
      301 gaagggacac caccttgacc gccttcttcg ccgccgcctc gcggtatttg cgcgcgcctt
      361 cggcccaggc ctccgagaag gaggcggcga gcagcgtcgc ttccacgggg atccagactt
      421 cgccgtcgcg gatcacgagc agctcgtcct gcaggctgat cagcccgcgg tcggcgtcct
      481 tcacgccggt gcggaacatc atgaacaggt ggccgggcac ctctaccatc gccgattcga
      541 tgccgaggtt ttcgtaggcg gcggcgagca gcaccgagag atcgtcgcag tcgccgctct
      601 tcagcttgag cgtctcgcgc ggaaactgga catagtcgac ctggtcggcg ctcaagcgcg
      661 agtagggcgt gttcgggtcg ggctgatagc gcagcccgag cgcgctcagg acgttgaaca
      721 ccgtggccgc ctggctgagc ggccggttca gcacgctctg cggcgggccg ccgtagcggt
      781 tcgcggcctc gcgcacgaaa ttgcgcagcg tgtcgtctct cggggtgacg aaggagccga
      841 tcatgtcgct gttcgaccag accagcgcgt tcttgccgta cagcgtcatc ggctgcgtga
      901 tctcctgcga gccgtcgcgg gaatcggcca gggcgagcgt gaccaccacc agcacccggg
      961 tgtcctcgtc gatgccgagc accttgttgt tgaaagtcgc gttgagcgtc agctcgagcg
     1021 agcccttcga cttgagcggc gggatttcct ggctgaccgg aaaatccatg tactccttga
     1081 tgaagaacga caggcgcagg cccttgtagt cctcggcgct gtcgtttcgc agcttgatgc
     1141 ggcccagcgg ctcggtggcg tactgcttgt gcgccgccac gaaaacgcgc tcgaggcgca
     1201 ggttttccat gacgatgcgg ccgccgctgc ccgactgcgc tttcttcttg cggtcgtaag
     1261 caccctcgag ctcgcgccgg ttggcgtcgc tcgggttcag cttgaccgcc cgcgtcaggg
     1321 ttgcgatcgc ggcgtcgtag gagcgctggt tgagctgcat ctgggcgagc atgaggtggg
     1381 gagcgtcgct gcgctcgttc aggcgcgcgg cgcgctccag gtgcgactgc gcgtcgacgt
     1441 aaagaccgtt atccgcgtag ctgcgggcga gcttgagctg cacgtcgtag gaatccggct
     1501 ccagcgcaac ggcgctcttg agcgtctcga tggcctcgcc gttcttgccc tggcggtagg
     1561 ccgcgtcggc gccgagcagc agcgcgtcga cgttcttcgg cgccaggcgc tgcgcctcgc
     1621 gcgccgcgtt ggcggcgtcg tgccagcgct cgagcgcggc gaaagtccgg gcctgcgcga
     1681 gccgcaggtc ggcgtcgccg ggcagcaggg cgacggcgcg ctcgagcggc gcgagcgccg
     1741 cggcgtggcg tgcggcgcgt atttcgaggt cggcgagctg gcggaatacc gccaccgtcg
     1801 cgtccggcag cgccgcggcg ctggcgagcg cttcgcgcgc cttgcgctcg tccttcagcg
     1861 cgaggtaggc ctcggccagc ccgccccagg ccgcgccgtg gcgcgcgtcg agcttggtgg
     1921 cgcgagcgaa ggcgatcacc gcctcctcgg gcttcttctg cttgagcgcg atgcggccca
     1981 ggatgtactg gccgcggctc tcctgcgccg ggctgccgac gagggacagc gcgatggtgc
     2041 gcgcctgatc catctcgccg agggagagat gaatctctgc gc
//
LOCUS       DASPXW010000234         3615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_949645, whole genome shotgun sequence.
ACCESSION   DASPXW010000234 DASPXW010000000
VERSION     DASPXW010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949645"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="VEL04_05470"
     CDS             complement(<1..834)
                     /locus_tag="VEL04_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HYT47848.1"
                     /translation="MTVATVASRALAGIDAPEVTVEVHLGPGLPAFHIVGLPDAEVRE
                     ARDRVRAALNHARFEFPARRITVNLAPAELPKDSSRFDLPIALGILAASGQLALEALQ
                     GQEFAGELSLTGELRSVRGALAMALSARGAGHAFVLPYENAPQAALAEGARILPARTL
                     LEVVAHLADEARLEEYVNGAQPVLPAYPDFRDVKGQQQVKRALEVAAAGAHSVLMVGP
                     PGAGKSMLAARFPGILPPLAEAEALEVAAVHSVSSNGFDAQRWGERPFRTPHHSASAP
                     AL"
     gene            1520..1720
                     /locus_tag="VEL04_05475"
     CDS             1520..1720
                     /locus_tag="VEL04_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47849.1"
                     /translation="MPRIVPRIRRVPEHVALRARTQMRVGKETEDMVMESGFSMIIAN
                     FRREKLEEVEKKLERLGSSAST"
     gene            complement(1872..2249)
                     /locus_tag="VEL04_05480"
     CDS             complement(1872..2249)
                     /locus_tag="VEL04_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47850.1"
                     /translation="MVEGRGLRLQADFLPSETVYNWASKVRSLGGAHMMEGGMGSMHG
                     WMIGLGGLIALLLVIVLIAAIVALVRLAARPHAPEQASSPRGTVVKVIVIVLAVIGAL
                     TLAGVAAGLVMHAGMMSVMGRCA"
     gene            2293..2667
                     /locus_tag="VEL04_05485"
     CDS             2293..2667
                     /locus_tag="VEL04_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47851.1"
                     /translation="MNRSLLRRVSLWLIAVLAFAQASVATAACQMDRGGLTTMLEVAH
                     DFGCETQVKPYFPQYPNRCIAHCTVDLQLSGLPAALVQAPADAAVLLLPRVERRGPRS
                     TGLDAAPPGAAPPRILLHSFLI"
     gene            2792..>3615
                     /locus_tag="VEL04_05490"
     CDS             2792..>3615
                     /locus_tag="VEL04_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HYT47852.1"
                     /translation="MAFAHAAALAQPLSLEDAQRRAVERSRQLAAQDATTAASREMAL
                     AAGQLPDPVLKLGVDNLPVTGADQFSLGRDFMTMRRIGVMQELTRSEKRQLRTQRFDR
                     EAEKSAAEKAAATASIQRDTALAWLDRYYVEALAGVVAEQKKQVELEVAAVEAAYRGG
                     RTNQADVFAANATLVALEDRASELRRRTQTATIILARWIGDGADAPLAPRPPLDSIRL
                     DAGALEAELAHHPQIAILAKQEEIAATEARLAQASSTPDWSVEVAYQQRGPAYSNMV"
BASE COUNT          669 a         1205 c         1138 g          603 t
ORIGIN      
        1 cagcgccggc gccgaagcgc tgtggtgcgg cgtgcgaaac ggccgctcgc cccagcgctg
       61 cgcgtcgaag ccgttgctcg acaccgaatg aaccgcggcg acctccagcg cctcggcctc
      121 cgcgagcggc ggcaggatgc cggggaagcg cgccgcaagc atcgatttgc ccgccccggg
      181 cgggccgacc atgagcacgc tatgggcgcc cgcggcagcg acctcgagcg ctcgcttgac
      241 ttgctgctgc cccttgacgt cgcgaaaatc cggatacgca ggcagaacag gctgcgcccc
      301 attgacgtat tcctcgaggc gagcctcatc cgcgaggtga gcaaccactt cgagtagcgt
      361 ccgcgcaggt aatatgcgag caccctcagc aagtgccgcc tgcggcgcat tttcgtaagg
      421 cagcacgaaa gcgtgaccag cgccgcgggc ggaaagcgcc atggcgagcg cgccgcgaac
      481 cgaacgcaat tccccggtta acgagagctc accggcaaat tcctggccct gcaaggcctc
      541 aagcgcaagt tgtccgctcg cagcgaggat gccgagcgct atcggcaggt cgaagcggct
      601 cgaatccttg ggcaactcgg ccggcgcgag gttgaccgtg atgcgtctcg ccgggaactc
      661 gaagcgcgcg tggttgagcg ccgcacgtac gcggtcgcgc gcttcgcgta cttcggcgtc
      721 aggcaagccg acgatatgaa acgccggcag ccccggcccg agatgcacct cgacggtgac
      781 ctcgggcgca tcgattcctg cgagcgcgcg gctcgcgacc gtggcgaccg tcattgaatt
      841 ttcccccggg gcaaataacg cgccgggact gccctgcgtt taccaacagc cttgctttag
      901 cctcgcctta tcaaacggga tcgccgcgcc gatgacaggc tgcatatgcg ctacggccgc
      961 cgcgagcgcg cctatagcat cgaatagcag cccttccatg cgcctgacct ccaacctgcg
     1021 ccggttaatg cacatgctgc gattgcggca ttgcgcgtcc cttctcgtgc gttaagagct
     1081 cgacttgcca cagcaaatcg aagatccaat tccatccttc gactgcaccc ggcggcggcc
     1141 tggaactgtg ttactgatcg tagggtccta caaaaggtag cccgagcgcc aaacgatgct
     1201 tcgagccgtg tccgtaattc tgctggcttt gtttttggcg agcttcgttc tcgctgataa
     1261 ccgagctgcg ccagatacag aacaggtcct gcctgccgct gtagcccaga tcgccggcga
     1321 atgcggcgat ggacctgggt ccggaggctg caacgcgatg tgcgcagcgt gcagcgcatt
     1381 tgccgctaac gccgtagtgc aatttccccg ggtcgatttc gacggacttc cgttcatgcc
     1441 ggcgtcgcgc ttcgccgatc cagcgcgcgc gccggacgcc actccaccta agcatctcct
     1501 cgcctgacgc gccggcttcg tgccgcgcat cgttccgcgt atccggcgcg tgccggaaca
     1561 cgtggcttta cgtgctcgta cgcagatgcg agtaggaaag gaaacggagg acatggttat
     1621 ggagagcgga ttcagcatga tcattgcaaa tttcaggcgc gagaaactcg aagaggtgga
     1681 aaagaagctc gagcgattgg ggtcgagcgc atcaacgtga gcaaagtgag agggttcggt
     1741 gaataccaca atcactttgc acacaactgg ctcgaaagcg aagtgcgggt ggagatatac
     1801 accaagaacc acgaagtcga gaccataacc tccgcggtca tggatgcggc ttccatcccg
     1861 ggattattgc ctcaggcgca acggcccatc acgctcatca tgccggcgtg catgacgagg
     1921 cctgccgcga ccccggccag cgtcagcgcg ccgatgacgg cgagcacgat tacgatcacc
     1981 ttcacgacgg tcccgcgcgg actgcttgcc tgctcgggcg catgcggccg agccgccagg
     2041 cgcaccagcg cgacgatggc ggcgatcagc acgatcacca ggagcagggc aatcaaccct
     2101 ccaaggccga tcatccatcc gtgcatcgat cccattccgc cttccatcat gtgggcgcct
     2161 ccgagcgatc tgaccttgct ggcccagtta tagacagtct cggaggggag gaaatccgcc
     2221 tgtaagcgca gtccgcgacc ttcgaccaac gaagattcga gttgcgttat agaatagcgc
     2281 cccccaagcc taatgaaccg ctcgctcttg cgccgcgtat cgctctggct catcgccgtg
     2341 ctggcgttcg cccaggcgag cgtcgcgacc gctgcctgcc agatggaccg cggaggcctg
     2401 acgacgatgt tggaggtcgc gcacgacttc ggctgcgaga cacaggtgaa gccgtatttc
     2461 ccgcagtacc ccaaccgctg cattgcgcat tgcacggtcg accttcagct ctcgggcctc
     2521 ccggccgctc ttgtgcaggc gccggccgat gccgcggttc ttcttctgcc gcgcgtcgaa
     2581 cgacgcgggc caagaagtac ggggctcgac gcggcgccgc cgggcgctgc gccgccgcgt
     2641 atcctgcttc attccttcct catttaatct ccgagccgcc tcctgaggcg gcaagcgatc
     2701 gcgaagtccg ccgcggcgat gtcgccgcga acatccaacg tcgtacggag aaaagatgtt
     2761 ttcaattcga aacgggttct gttgtgccgg catggcgttc gcccatgcgg cggcattggc
     2821 gcagccgctc tcgcttgaag acgcacagcg tcgcgcggtc gagcgctcgc ggcagctcgc
     2881 ggcgcaggac gctacgaccg ccgcatcgcg ggaaatggcg ctggcggccg gccagttgcc
     2941 cgaccccgtg ctcaagctgg gagtcgacaa tcttcccgtc accggtgcgg accagttcag
     3001 cctcgggcgc gatttcatga ccatgcggcg catcggcgtg atgcaggagc tgacccgcag
     3061 cgaaaagcgc cagctgcgca cgcagcgctt cgatcgcgag gccgaaaagt cggccgccga
     3121 gaaagctgcc gcgaccgcgt cgattcagcg tgataccgct ctggcctggc tcgatcgata
     3181 ctacgtggag gcgcttgccg gcgtcgttgc cgagcagaag aagcaggtcg agctggaggt
     3241 cgccgccgtc gaggccgcgt accggggcgg gcgcacgaat caggcggacg ttttcgccgc
     3301 caatgcaacg ctcgttgccc tcgaggatcg ggcaagcgag ctcaggcgtc ggacccagac
     3361 cgccacaata atcctcgcgc gctggatcgg cgacggcgcc gacgcgccgc ttgcgccccg
     3421 gccgccgctc gacagcatcc ggctcgatgc cggagcgctc gaagccgagc tggcgcatca
     3481 tcctcagatc gcgatcctcg ccaagcagga agagatcgcg gcaaccgaag cgcggctcgc
     3541 ccaggcgagc agtacccccg actggagcgt cgaggtggcg taccagcagc gcggcccggc
     3601 atactcgaac atggt
//
LOCUS       DASPXW010000235         3117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1581031, whole genome shotgun sequence.
ACCESSION   DASPXW010000235 DASPXW010000000
VERSION     DASPXW010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1581031"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..459
                     /locus_tag="VEL04_05495"
     CDS             127..459
                     /locus_tag="VEL04_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HYT47853.1"
                     /translation="MTKSELIARLAQRYPQLVAKDAEYAVKMILDAMTQSLLSGHRIE
                     IRGFGSFGLNYRPPRTGRNPKSGEKVQVPEKYVPHFKAGKELRERVDGATPDAATAPR
                     ERASQPVR"
     gene            475..750
                     /locus_tag="VEL04_05500"
     CDS             475..750
                     /locus_tag="VEL04_05500"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HYT47854.1"
                     /translation="MRIVTWALRLIVFLLLAAFAAKNVEPVTLRFYFDLALQAPLVVV
                     LFGCFAAGALFGILALVGTLLRQRRQISLLKEQTSSAEAPQPPAAGL"
     gene            753..1919
                     /gene="lapB"
                     /locus_tag="VEL04_05505"
     CDS             753..1919
                     /gene="lapB"
                     /locus_tag="VEL04_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HYT47855.1"
                     /translation="MEFDYWWLLGLLPLFFGFGWIAARIDIKHLVRDSRALPRSYFKG
                     LNFLLNEQPDKAIEAFIEVVKVDPETVELHFALGSLFRRRGEFDRAIRMHQNLLERAD
                     LGPEQKGVALAELGEDYLKAGILDRAEEVFQKLEKTPHAIAARRHLLDIYEQEKDWSR
                     AIEMTRLVESDPRELAQYLCEQAASEAAQSHPDAARRHLEAALEANRKCVRASLLLGD
                     LEKDESKLDLAIEHWKRIESQHPAYLALVAQRLAEAHRDAGRAEEGLRLLAGYLERYP
                     SLDLLDSVFQQTLEAKGADAAYALVRDELRRNPTLLGLDRLLEAQIIGTDVPDRRRDL
                     ELVKNLVHSHTRKLARYRCESCGFKARQFHWRCPACGGWETYPPRRTEEFELTP"
     gene            1916..3040
                     /locus_tag="VEL04_05510"
     CDS             1916..3040
                     /locus_tag="VEL04_05510"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013390990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HYT47856.1"
                     /translation="MNVTVIGTGYVGLVSGACLADAGNSVFCLDVDEEKIARLNAGRI
                     PIFEPGLEPIVRRNAQAGRLRFSTDVAASVEHGELQLIAVGTPPGEDGSADLQHVVAA
                     ARAIGRHMRGYKIVVTKSTVPVGTADKVKQAIIQELKKRGTGSDFTVVSNPEFLKEGA
                     AVEDFMRPDRIILGSDDARAIAALRQLYAPFQRNRERIIVMGVRSAELTKYAANAMLA
                     TRISFMNEVANLAEALGADIEEVRRGIGSDPRIGYQFLYAGAGFGGSCFPKDVKALQR
                     SADDAGRPLRLLAAVEEVNQAQKHVLAEKIRARFGGSLSGKKIALWGLAFKANTDDMR
                     EAPSLTLIDVLTQGGAAVQAYDPAAVRAHGIEYHPIGRKP"
BASE COUNT          538 a         1065 c         1063 g          451 t
ORIGIN      
        1 gcggcgagcg ccggcgcgac caacctcggc gcgctgctgc gggcgaagct cgacaaggga
       61 aacaccccgc agcagtaagc gggaaacggc accggtcagc gctacgaaaa ggggagaagg
      121 gtcgccatga ccaagtcgga actgatcgcg cgcctcgccc agcgctatcc gcagctggtg
      181 gcgaaggacg ccgagtacgc ggtgaaaatg atcctcgatg cgatgacgca gagcctgctc
      241 agcggccacc gcatcgagat ccgcggcttc ggcagcttcg ggctgaacta ccgcccgccg
      301 aggaccggcc gcaatcccaa gtcgggcgag aaggtgcagg tgcccgagaa gtacgtgccg
      361 cacttcaagg cgggcaagga actgcgcgag cgcgtcgacg gcgcgacccc ggacgccgcg
      421 actgccccgc gggagcgcgc ttcgcagccg gtccgctagc acatcaaggg cgcgatgcgc
      481 atcgtcacct gggcgctccg cctgatcgta ttcctcctgc tggccgcgtt cgccgccaag
      541 aacgtcgagc cggtgaccct gcgcttttat ttcgacctgg cgctgcaggc ccccctggtc
      601 gtggtgctgt tcggctgctt cgcggcggga gcgctgttcg gcatcctcgc gctggtggga
      661 acgctgttgc gccaacgcag gcagatatcg cttctcaagg aacagacttc ttcagctgag
      721 gcaccgcagc ctccggcggc cggactctag ccatggaatt cgactactgg tggctgctcg
      781 gcctgctgcc gctgttcttc ggcttcggct ggatcgcggc gcgcatcgac atcaagcacc
      841 tggtgaggga ctcgcgcgcg ctgccgcgct cctacttcaa ggggctcaac ttcctgctca
      901 acgagcagcc cgacaaggcg atcgaggcct tcatcgaggt ggtcaaggtc gaccccgaga
      961 ccgtcgagct gcacttcgcg ctcggcagcc tgttccggcg ccgcggcgag ttcgaccggg
     1021 cgatccgcat gcaccagaac ctgctcgagc gcgccgacct cgggcctgag cagaaggggg
     1081 tggcgcttgc cgagctcggc gaggattacc tcaaggcggg gattctcgac cgcgccgagg
     1141 aggtgttcca gaaactggaa aagaccccgc acgccatcgc ggcgcggcgg catctgctgg
     1201 acatctacga gcaggaaaag gactggtcgc gcgcgatcga gatgacgcgc ctggtcgagt
     1261 ccgatccgcg cgagctggcg caatacctgt gcgagcaggc cgcctccgag gccgcgcagt
     1321 cgcatccgga cgccgcgcgg cgccatctcg aggccgcgct cgaggccaac cgcaagtgcg
     1381 tgcgcgcgag cctgctgctc ggggacctgg aaaaggacga gagcaagctg gacctggcga
     1441 tcgagcactg gaagcgcatc gaatcgcagc acccggcgta tctggcgctg gtggcgcagc
     1501 gcctggccga ggcgcatcgc gacgcgggcc gggccgagga gggactgcgc ctgctcgccg
     1561 gctacctcga acgctatccg tcgctcgacc tgctcgacag cgtgttccag cagacgctcg
     1621 aggccaaggg tgccgacgcc gcctacgcgc tggtgcgcga cgagctgcgg cgcaacccga
     1681 cgctgctcgg cctcgaccgc ctgctcgagg cgcagatcat cggcaccgac gtgcccgacc
     1741 gccggcgcga cctcgagctg gtgaagaacc tcgtgcatag ccatacccgc aagctcgccc
     1801 gttaccgctg cgagagctgc ggcttcaagg cgcgccagtt ccactggcgc tgcccggcct
     1861 gcggcggctg ggagacctac ccgccgcgcc gcaccgagga atttgagctc acgccatgaa
     1921 cgtgacggtc atcggaacgg gctacgtggg actggtcagc ggcgcctgcc tcgcggacgc
     1981 cggcaactcg gttttctgcc tcgatgtgga cgaggagaag atcgcccggc tcaatgcggg
     2041 caggattccc atcttcgaac ccggtctgga gccgatcgtc aggcgcaacg cgcaggccgg
     2101 gcggctgcgc ttctcgaccg atgtcgcggc gagcgtggag cacggcgagc tgcagctgat
     2161 cgcggtcggc acgccgcccg gcgaggacgg ctcggccgac ctgcagcacg tggtcgctgc
     2221 agcgcgcgcc atcggccggc acatgcgcgg ctacaagatc gtggtgacca agtcgacggt
     2281 tccggtgggc accgcggaca aggtcaagca agccatcatc caggagctga agaagcgcgg
     2341 caccggcagc gatttcaccg tggtgtcgaa cccggaattc ctcaaggaag gcgccgcggt
     2401 cgaggacttc atgcgccccg accgcatcat cctcggctcc gacgacgcgc gcgccatcgc
     2461 ggctctgcgc cagctgtatg cgccgttcca gcgcaaccgc gagcgcatca tcgtgatggg
     2521 ggtgcgctcg gcggagctca ccaagtacgc cgccaacgcg atgctcgcca cccgcatctc
     2581 gttcatgaac gaggtggcga acctcgccga ggcgctcggc gccgacatcg aggaggtgcg
     2641 gcgcggcatc ggctcggatc cgcgcatcgg ctaccagttc ctgtacgccg gcgccggctt
     2701 cggcggctcg tgcttcccga aggacgtcaa ggcgctgcag aggagcgccg acgatgcagg
     2761 gcggccactg cgcctgctcg ccgcggtcga agaggtgaac caggcgcaaa agcacgtgct
     2821 cgccgagaag atccgcgcgc gctttggcgg ctccctgtcg ggaaagaaga ttgcgctctg
     2881 gggcctcgcc ttcaaagcca ataccgacga catgcgcgag gcgcccagcc tcaccctcat
     2941 cgatgtcctg acgcagggcg gcgcagcggt gcaggcttat gatccggcgg ccgtgcgggc
     3001 gcacggcatc gaataccacc ccatagggag aaaaccataa cttcacgcag aggcctgcaa
     3061 gcccccgcca cgtccgaagc gcgcgtgctg gtggtcggcg acgtgatgct cgaccgc
//
LOCUS       DASPXW010000236         3007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_195442, whole genome shotgun sequence.
ACCESSION   DASPXW010000236 DASPXW010000000
VERSION     DASPXW010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195442"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="VEL04_05515"
     CDS             complement(<1..902)
                     /locus_tag="VEL04_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HYT47857.1"
                     /translation="MMGTAELRNHERELHQFQLRVGIAGIAVLVAFGLLAARFFYLQV
                     VQHDVYQAKAEDNRISIVPVLPNRGLLVDRNGLVLARNYSGYTLEIFPRKVSNLEQTI
                     DELAELIEIQPRDRARFRKLVAETRNAESLPIRTRLSDEEAAKFAANRYRFEGVELKA
                     RLFRQYPYGEIASHAIGYLGRIDQADQARLAEEGLDANYRGSDFIGKSGVEASYEQEL
                     HGTTGFEQVEIDAAGRGIRTLSRTPSQPGNNITLTLDMRLQQVAEKAFGERRGALVAI
                     EPASGDVLALVSKPDFDPNQFVDG"
     gene            complement(913..1422)
                     /gene="mreD"
                     /locus_tag="VEL04_05520"
     CDS             complement(913..1422)
                     /gene="mreD"
                     /locus_tag="VEL04_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018441279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HYT47858.1"
                     /translation="MFQDRPQTILLPVRTSTIIASFALALFLNFLPWRDLRLTPDFIA
                     LVLTFWCVRQPRLVGLGVGWALGLLSDAGNGVLLGQHALAYSLLAFLAVWLSRRILWF
                     GPGLQALHVALILTVGQCAVLLVRLAAGNAFPGWPIFVGPLAGAALWPAVTWLLLLPQ
                     RRTEREQAI"
     gene            complement(1409..2344)
                     /gene="mreC"
                     /locus_tag="VEL04_05525"
     CDS             complement(1409..2344)
                     /gene="mreC"
                     /locus_tag="VEL04_05525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HYT47859.1"
                     /translation="MDHTPPPFFKRGPAPLVRLFFFASLSLALLVIDARFRYAEGLRG
                     WLALGVYPIQRAALAPIDLGEHIAGYFSTQARLVAENERLRAKALEYSQDAQRYQAAE
                     AEAAELRRLIGAGERLELHAAQAEVLYAGRDPYAQKIFIDRGATQGVRPGSPVADEAG
                     VVGQVTRVHPLVSEVTLLTNSDQATPVQVVRNGLRAIAFGGGASGMLELRFTATNAEI
                     QSGDRLVTSGIDGTYPQGLPVATVVHIERDAEHTFARVLCRPAAGVDRGRYVLVLANE
                     SARPPRPDEAQPGKRAEKSRRARVRETAKDGDVSR"
     gene            complement(2386..>3007)
                     /gene="mreB"
                     /locus_tag="VEL04_05530"
     CDS             complement(2386..>3007)
                     /gene="mreB"
                     /locus_tag="VEL04_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HYT47860.1"
                     /translation="IEEPMAAAIGADLPIAEATGSMVVDIGGGTTEVGVISLGGMVYA
                     GSVRVGGDKFDEAIVNYIRRNYGMLIGETTAENVKKEIGSAFPGSEVREMEVKGRNLA
                     EGIPRSFTISSNEILEALTEPLNAIVSAIKSALEQTPPELGADIAEKGMVLTGGGALL
                     RDLDRLLMEETGLPVIVADDPLTCVVRGSGKALEQMEHFGPIFTND"
BASE COUNT          476 a         1022 c         1039 g          470 t
ORIGIN      
        1 atgccgtcga cgaactggtt cggatcgaag tccggcttcg aaaccagcgc gagcacgtcg
       61 ccgctcgccg gctcgatggc gaccagcgcg ccgcggcgct cgccgaaggc cttctccgcc
      121 acctgctgca ggcgcatgtc gagcgtcagg gtgatgttgt tgccgggctg cgagggcgtg
      181 cgcgagagcg tgcggatgcc gcgtcccgcg gcgtcgatct ccacctgctc gaatcccgtc
      241 gtgccgtgca gctcctgctc gtagcttgcc tcgacgccgc tcttgccgat gaagtcgctg
      301 ccgcgatagt tcgcgtcgag cccttcctcc gcgagccgcg cctggtcggc ctggtcgatg
      361 cgccccaagt agccgatggc gtgcgaagcg atctcgccgt acgggtactg gcggaacagg
      421 cgcgccttga gctcgacgcc ctcgaaccgg tagcggttgg cggcgaactt cgccgcttct
      481 tcgtcggaga gccgggtgcg gatcggcagg ctctcggcat tgcgcgtttc ggcgacgagc
      541 ttcctgaagc gcgcgcggtc gcgcggctgg atctcaatca gctccgcgag ctcgtcgatg
      601 gtttgctcca ggttagaaac cttgcgcggg aagatctcca gggtgtaccc ggaatagttt
      661 cgcgccagca ccaggccgtt gcggtcgacg agcaggccgc ggttcggcag caccggaacg
      721 atggagatgc ggttgtcctc ggcttttgcc tggtagacgt cgtgctgcac cacctggagg
      781 tagaagaagc gcgcggcgag caagccgaac gcgaccagga ccgcgatgcc ggcgatgccg
      841 acgcgcagct ggaactggtg gagctcgcgc tcgtggttcc gcagctcagc cgtgcccatc
      901 atggtcggca tgctagatcg cctgctcgcg ctcggtgcgc cgctgcggca gcagcaggag
      961 ccaggtgacg gcgggccaga gcgccgcgcc ggcgagcggc ccgacgaaga tcggccatcc
     1021 cggaaacgcg ttgcccgccg ccagccgcac cagcagcacg gcgcactgcc cgacggtcag
     1081 aatcagcgcg acatgcagcg cctgcaggcc aggcccgaac cacaggatgc ggcgggagag
     1141 ccacaccgcg agaaaggcga gcagcgaata ggcgagcgca tgctgcccga ggagcacgcc
     1201 gttgccggcg tcggagagca ggccgagcgc ccagccgacg ccgaggccga ccagccgcgg
     1261 ctggcgcacg caccagaagg tcagcaccag cgcgatgaag tcgggcgtga gccgcaagtc
     1321 gcgccatggc aggaagttca agaacaaagc gagagcgaac gaagcgatga tggtgctcgt
     1381 ccgcaccggc agcaggatgg tctgaggcct atcttgaaac atcgccgtct ttcgccgttt
     1441 ctctcacccg cgcgcggcgc gatttctcgg cgcgcttgcc cggctgcgct tcgtcggggc
     1501 gcggcggccg cgccgactcg ttcgccagca ccagcacgta gcggccgcgg tcgaccccgg
     1561 ctgcgggcct gcacagcacg cgcgcaaaag tgtgctcggc gtcgcgctcg atgtggacca
     1621 cggtcgcgac cggcagtccc tggggatagg tgccgtcgat tcccgaggtc acgagtcggt
     1681 caccgctctg gatctcggcg tttgtcgcag tgaagcgcag ctcgagcatc cccgaggcgc
     1741 cgccgccgaa agcgatggcg cgcaacccgt tgcgcaccac ctgcaccggg gtcgcctggt
     1801 cggagttggt gagcagggtg acttcggaaa cgagcggatg cacgcgcgtc acctggccga
     1861 caacgccggc ctcgtcggcc accgggcttc ccgggcgcac gccctgcgtt gcgccgcggt
     1921 cgatgaatat tttctgggcg taggggtcgc ggcctgcata gagcacttcg gcctgggccg
     1981 cgtgcagctc gaggcgctcg ccggcaccga tcagccggcg cagctctgcc gcctcggcct
     2041 ccgcggcctg gtagcgctgc gcgtcctgcg aatattcgag cgccttggcg cgcagccgct
     2101 cgttttccgc gaccagccgc gcctgggtgg agaaatagcc cgcgatgtgc tcgccgagat
     2161 cgatcggtgc cagcgccgcg cgctgaatcg ggtacacgcc gagcgcaagc cagccacgca
     2221 ggccctcggc atagcggaaa cgagcgtcga tcacgagcag ggccaaggag agcgaggcga
     2281 agaaaaacag cctgacgagc ggcgccggcc cgcgcttaaa gaagggcggg ggggtatggt
     2341 ccatctgcga agggaccggg ggcccctttt atcccccggg ggcgctcaat cgttggtgaa
     2401 gatcgggccg aagtgctcca tttgctcgag cgccttgccc gagccgcgca cgacgcaggt
     2461 gagcgggtcg tcggccacga tcaccggcag acccgtctcc tccatcagca ggcggtcgag
     2521 gtcgcggagc agcgctccgc ccccggtgag caccatgcct ttctcggcga tgtcggcgcc
     2581 gagctcaggc ggcgtctgct cgagcgccga tttgatcgcc gagacgatgg cgttcaacgg
     2641 ctcggtcagc gcctcgagga tctcgttcga tgagatggtg aagctgcgcg ggatgccttc
     2701 ggcgagattg cggcccttga cctccatctc gcgcacctcg ctgcccggga acgcggagcc
     2761 gatttccttc ttcacgttct cggcggtggt ctcgccgatc agcatgccgt aattgcgccg
     2821 gatgtaattg acgatcgcct cgtcgaactt gtcgccgccc acgcgcaccg agcccgcata
     2881 caccatgccg ccgagcgaga tcacgccgac ctcggtcgtg ccgccgccga tatccaccac
     2941 catcgagccg gtggcctcgg cgatcggcag gtccgctccg atcgcggccg ccatgggctc
     3001 ttcgatc
//
LOCUS       DASPXW010000237         2137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1708349, whole genome shotgun sequence.
ACCESSION   DASPXW010000237 DASPXW010000000
VERSION     DASPXW010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1708349"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..393)
                     /locus_tag="VEL04_05535"
     CDS             complement(61..393)
                     /locus_tag="VEL04_05535"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HYT47861.1"
                     /translation="MTATAFGVLLVVVSTMVEGFGQIFLKKSVLELARRRFWVLLGVC
                     VLIVEVLLYSGALRFLAVSTAFPLTSLSFVMVTLLSRWLLGEHVTPLRWFGVGLILIG
                     TSLIVAEA"
     gene            complement(390..803)
                     /locus_tag="VEL04_05540"
     CDS             complement(390..803)
                     /locus_tag="VEL04_05540"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HYT47862.1"
                     /translation="MTTGARFPIRLVIGLAVAIALDTALQLVWKTGVAAIPETSSLWE
                     TVLAVASAPIFLLIVALMAAQLVNWLKVLDHADLSYAKPFTSLGYVTVCVLSVLWLGE
                     RIAPLQIVGILIVVAGVSCVSMTPRTTLRPGAASE"
     gene            complement(963..1292)
                     /locus_tag="VEL04_05545"
     CDS             complement(963..1292)
                     /locus_tag="VEL04_05545"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HYT47863.1"
                     /translation="MSAEAENKGIDQVTFEAGFKARIMGIDGTWWRECYIEVISPTGA
                     KITVEGSVENLKLNEFFLMLSSVGNAHRKCELVYVKGDEIGVRFLQKTEGKRRRPTFN
                     ENEEFVP"
     gene            complement(1442..>2137)
                     /locus_tag="VEL04_05550"
     CDS             complement(1442..>2137)
                     /locus_tag="VEL04_05550"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HYT47864.1"
                     /translation="VVPPELLSAVERALPHVKMSESYGLTEGGSPFRAPVDGRPVPRG
                     SVGVKAPDVEVKLIDASGREHPTDGELSIRSPYVCLGYHNQPEVTAEKLTDGWLRTGD
                     IFHRDAAGFYFFRSRVDDMFSCGGENIYPKEVENILFTHPDVADAVVAPVPHGVKGFV
                     PAAMVIPRAGARLTGAALKTHFLARGPAYAHPRFIAIVPQFPLNGAGKIDRGAVRRAL
                     EAEYRGQDAGAAS"
BASE COUNT          386 a          704 c          707 g          340 t
ORIGIN      
        1 cacgggcgta gagattgctt cgtcgcgctg cgtgctcctc gcaatgacat ttgacgcgcg
       61 tcacgcctcg gcgacgatca ggctggtgcc gatcaggatc aggccgacgc cgaaccagcg
      121 cagcggagtc acgtgctcgc ccagcagcca tctcgagagc agcgtgacca tgacgaagct
      181 caggctggtg agcggaaagg cggtcgagac cgcgaggaag cgcagcgcgc cggaatagag
      241 gaggacctcg acgatcagca cgcagacgcc gagcagcacc cagaaccgcc ggcgcgcgag
      301 ctccagcacg gatttcttga ggaaaatctg gccgaaaccc tcgaccatgg tgctgaccac
      361 gacgagcagc acgccgaacg cggtcgcggt cattcggacg ccgctccagg gcgcagcgtc
      421 gtgcgcggcg tcatgctcac gcaggagacg cccgcgacca cgatgaggat gccgacgatc
      481 tgcagcggcg cgatgcgctc gcccagccac agcaccgaca ggacgcagac cgtgacatag
      541 ccaagcgagg tgaacggctt ggcgtagctg agatcggcgt gatcgagcac cttgagccag
      601 ttgacgagct gcgccgccat cagcgcgacg atcagaagga agatgggcgc gctcgccact
      661 gcgaggaccg tctcccacag cgaggacgtc tcgggaatcg cggcgacgcc ggtcttccag
      721 acgagctgca gcgcggtgtc gagcgcaatc gccaccgcga gtccgatcac cagcctgatt
      781 ggaaagcgcg cccccgtggt catgacgaag cattgagcat agcggccgcg ggctcgcaag
      841 tgcgcaatgt ttacctgtca ttccggggcg cgcggaacgc gcgagcccgg aatccatctt
      901 cgattcccgt ggttatggac tccgggcccg cccctgcggg gcgtcccgga atgacaaagg
      961 tcttacggga cgaattcctc gttctcgttg aacgtcgggc ggcggcgctt gccttccgtc
     1021 ttctgcagga agcgcacgcc gatctcgtcg cccttgacgt agacgagctc gcatttgcgg
     1081 tgggcattgc cgaccgacga cagcatcagg aagaactcgt tgagcttgag attttcgacg
     1141 ctgccctcga ccgtgatctt ggcgccggtc ggcgagatca cctcgatgta gcactcgcgc
     1201 caccaggtgc cgtcgatgcc catgatgcgc gccttgaagc cggcctcgaa tgtgacctga
     1261 tcgatacctt tgttttctgc ttccgcactc atgaccgaac cctcgcgcac caggccatcc
     1321 gcgatagagt taggatcgga aggttaatgt ccgcttgacg tgggtggaat taatgccgca
     1381 ggcgaggtcg cgccctggca ttttacccgc gcgcccaatg ccataggccg gcgccgaacc
     1441 cctacgacgc ggcgcctgcg tcctgcccgc ggtactccgc ctcgagcgcg cggcgcaccg
     1501 ccccgcggtc gatcttgccg gcgccgttga gcggaaactg cggcacgatc gcgatgaagc
     1561 gcggatgcgc ataggccgga ccgcgcgcca gaaaatgggt cttgagcgcc gcgccggtga
     1621 ggcgcgcgcc ggcgcgcgga atcaccatgg cggccggcac gaagcctttc acgccgtgcg
     1681 gcaccggcgc gaccacggca tcggcgacgt cgggatgggt gaacaggata ttttcgactt
     1741 ccttgggata gatgttctcg ccgccgcagc tgaacatgtc gtcgacccgg ctgcggaaga
     1801 aatagaagcc cgcggcatcg cggtggaaga tgtcgccggt gcgcagccag ccgtcggtga
     1861 gtttttccgc cgtgacctcg ggctggttgt gatagccgag acacacatag gggctgcgga
     1921 tcgaaagctc cccgtcggtc ggatgctcgc ggccgctcgc gtcgatgagc ttcacctcga
     1981 catcgggcgc cttgacgccg acgctgccgc gcggcaccgg ccggccgtcg accggcgcgc
     2041 ggaacgggct gccgccttcg gtgaggccgt agctctccga catcttcacg tgcggcagcg
     2101 cgcgctcgac ggcgctcaac agctccggcg gcaccac
//
LOCUS       DASPXW010000238         2272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_701410, whole genome shotgun sequence.
ACCESSION   DASPXW010000238 DASPXW010000000
VERSION     DASPXW010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701410"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /gene="fdhE"
                     /locus_tag="VEL04_05555"
     CDS             <1..877
                     /gene="fdhE"
                     /locus_tag="VEL04_05555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HYT47865.1"
                     /translation="PPGEIPFLLLPERNAFQARAARLSALAEDHALADYLRFLAALAR
                     AQHESMALFRHVLLPEAQQLERCREHGMPPLGTRGWDRNPVWREALRCILKELEAGAL
                     PETARATMARLKDTDDASLERVAETLLAGDNDDKLDRAAIPFIGAALQAYWLHLVTTL
                     GAGAFGRLETPHLCPACGSAPMVSVVCIGASAHDYGLRYLTCPLCNTQWHAVRIKCVF
                     CDTTKGITYYGIEGGSAAVKAESCDACHGYLKILYMDKDPNVEALADDVASIALDVLM
                     AESGITRRGTNFFLF"
     gene            983..>2272
                     /gene="selA"
                     /locus_tag="VEL04_05560"
     CDS             983..>2272
                     /gene="selA"
                     /locus_tag="VEL04_05560"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HYT47866.1"
                     /translation="MPSVDRVLQIPAVQALGAQYSHTAVVHAIRSLLSEMRQAMLEGA
                     ALPAEARQALSLARHLQARLEAAFQPSLRRVFNLTGTVLHTNLGRALLPSEAVEAVQA
                     VMAQSCNLEYDLAPGARGDRDTHVEALLAELTGAEAATVVNNNAAAVLLVLATLAARK
                     EVIVSRGELIEIGGSFRIPTIMAHAGCRLREVGTTNRTHLADFEQAIGARSALLMKVH
                     ASNYTIQGFTATVPEKELATLAHARELPFVVDLGSGALVDLSQYGLPKEPTPQETLAH
                     GADLVTFSGDKLLGGPQAGIIVGRAELVKTITRDPLKRALRVDKMTLAALEAVLKLYR
                     QPEKLAQRLPTLRLLTRPAADIEALCRRVEPAVVQAVRNAAHVSVVPCHSQIGSGALP
                     VELLPSTALALAPLKRGRGEGTALRQLAAAFRALPVPV"
BASE COUNT          370 a          758 c          759 g          385 t
ORIGIN      
        1 gccgccgggc gaaataccgt tcctgctcct gccggagcgc aacgcttttc aggcgcgcgc
       61 cgcgcgtctg agcgccctgg ccgaagacca cgccctggcg gattacctcc ggttcctggc
      121 ggcgctggcg cgcgcgcagc atgagtcgat ggcgctgttc cgccatgtcc tgctgccgga
      181 ggcgcaacag ctcgaacgct gccgcgaaca cgggatgccg ccgcttggga cccggggctg
      241 ggaccgcaat cccgtgtggc gcgaggcgtt gcgctgtatt ctcaaggaac tcgaagccgg
      301 ggcactgccg gagacggcgc gcgcgacgat ggcgcggctc aaggacacgg acgacgcgtc
      361 gctcgaacgc gtggccgaga cgctgctcgc cggcgataat gatgacaagc tcgatcgggc
      421 ggcgatccca ttcatcggcg cggcgctaca ggcgtactgg ttgcatctgg tcacgacgct
      481 cggggccggt gccttcggcc ggctcgagac cccccatctc tgcccggcgt gtggttccgc
      541 gcccatggtg agtgtcgtgt gcatcggcgc ctcggcccac gactacggcc tgcgctatct
      601 gacctgcccg ctctgcaaca cgcaatggca cgcggtgcgt atcaagtgcg tcttctgcga
      661 tacgaccaag ggcattactt actatggcat cgagggcggc agcgccgcgg tcaaggccga
      721 gagttgcgat gcctgccatg gttatctcaa gatcttgtac atggacaagg acccgaacgt
      781 ggaggcgctc gcggatgacg tggcgagcat cgcgctcgat gtcctgatgg ccgaatccgg
      841 tatcactcgc agggggacga atttcttctt attttaggag gaagtgagta gctcggtaaa
      901 cgcattgtag tgcgtgtgag tattcacgct ataggcggaa ctgtccttgg tccaagcgca
      961 gaagccgcaa acagctgctg tattgccgtc ggtcgatcgt gtgctccaga tacctgcggt
     1021 gcaggcactg ggcgcacagt atagccacac ggccgtggtc cacgccatcc gttccctgct
     1081 ctccgaaatg cgtcaggcca tgctggaggg tgccgcgctg cccgcggaag cgcggcaagc
     1141 gctgtctctc gcgcgacacc tccaggctcg cctcgaggcc gcgttccagc cctctctgcg
     1201 gcgcgtgttc aacctcaccg gcacagtgct gcacaccaat ctcgggcgcg cgctgttgcc
     1261 gtcggaggcc gtcgaagctg tgcaggcggt catggcgcag tcgtgcaatc tcgagtacga
     1321 cctcgcacct ggcgcgcgtg gcgaccgcga cacccatgtc gaagcgctgc tcgccgaact
     1381 caccggcgct gaggccgcca cggtggtgaa caacaacgcc gccgcggtat tgctggtgct
     1441 cgctacgctc gcggcgcgca aggaggtgat cgtctcgcgc ggcgagctca tcgaaatcgg
     1501 cggatcgttc cgcattccca cgatcatggc gcatgcgggc tgccgcctgc gcgaggtcgg
     1561 caccaccaat cgcacccatc tcgccgattt cgagcaggcc atcggcgcac gcagtgcgct
     1621 gctcatgaag gtgcacgcca gcaactacac gatccagggt ttcaccgcga cagtgcctga
     1681 gaaggaactc gcgacgctcg cgcatgcccg ggagcttcca ttcgtggtgg acctcggcag
     1741 cggcgcgctg gtggatctga gccaatatgg actgccgaaa gaaccgaccc cgcaggagac
     1801 gttggcgcac ggcgccgatc tcgtgacctt cagcggcgac aagctcctcg gcgggccgca
     1861 ggccggcatc atcgtcgggc gcgccgagct cgtgaaaaca atcacacgcg accccctcaa
     1921 gcgtgcgctg cgtgttgaca agatgacgct cgccgcgctc gaggcggtgc tcaagctcta
     1981 tcgtcagccg gagaagctcg cgcagcgtct gccgacgttg cggctgctca cgcgcccggc
     2041 ggccgacatc gaggcactgt gccgacgtgt cgagcccgcg gtggtgcaag cggtacgcaa
     2101 tgcggcgcac gtgagtgtcg tgccgtgcca cagccagatc ggcagtggcg cgctgccggt
     2161 ggagctgctg ccgagcacgg cgctcgcgct cgcgccgctc aagcgcgggc gcggcgaggg
     2221 caccgccctc aggcagttgg cggccgcgtt ccgcgcgctt cccgtcccgg tg
//
LOCUS       DASPXW010000239         3395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1207893, whole genome shotgun sequence.
ACCESSION   DASPXW010000239 DASPXW010000000
VERSION     DASPXW010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207893"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1251)
                     /locus_tag="VEL04_05565"
     CDS             complement(<1..1251)
                     /locus_tag="VEL04_05565"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HYT47867.1"
                     /translation="MKGLLKTFSLLIAAALLHACASGPASEGASPHAAQSPIPDQGTP
                     PAPLPNPPVAAAPAPAATAPFEGERRALKERARLPQVDLTLMPGDLWQRIRRGFAIPD
                     IDNKLVEQHTAYYAARPEYLQRVFDRSRLYLYHIVEEIEKRSLPTELALLPMVESAFN
                     PMAYSRSHASGLWQFIPGTGRRYELKQNWWYDGRRDIVDSTTAALDYLTFLYQMHGDW
                     QLALASYNWGENAVARAIAKNRAAGKPTDYEHLKMPAETRNYLPKLQALKNIIANPEP
                     FGVLLDPIPNQPYFASFTRLVDIDVQLAAKLAEMPVEEFMALNPGFSRPLIRAAMTPR
                     IVLPADKIDVFHDNLSKLEGESLVSWKTYYPKKGETFESIAKKHGLMLGQLKEVNGIG
                     PRVRVVPTMLVVPVNGQGASLQHMP"
     gene            complement(1248..2027)
                     /gene="gloB"
                     /locus_tag="VEL04_05570"
     CDS             complement(1248..2027)
                     /gene="gloB"
                     /locus_tag="VEL04_05570"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HYT47868.1"
                     /translation="MLQGVPLSIVPVAAFKDNYVWTLRNETHAAVVDPGEARPVLDYL
                     AREKLELAAILATHHHPDNVGGIAELLQKFRVPVYGPKNEPIATLTRPVSEGDTVSVA
                     QLGVAFSVLDIPGHTRAHIAYYGAGALFCGDTLFACGCGRLFEGTAEQMYASLKKLAA
                     LPDDTKVYCGHEYTLANIGFAKTVEPGNTALQARESRDRQLRQSGRPTLPSTLREEKA
                     TNPFLRCLEPAVVESANKYLGARIADPVRVFAAIRDWKNRF"
     gene            2026..2808
                     /locus_tag="VEL04_05575"
     CDS             2026..2808
                     /locus_tag="VEL04_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYT47869.1"
                     /translation="MYRHSFVQNGAQKYNGGRSSLSGWFATPQGAYVLGWELAQYDSA
                     VDDVFGFRAVQIGLPEVDFLRQNRIQFRFTLALEPGAALAADPLQLPLASQSVDLIAL
                     PHVLEFHPNPHEVLGEAERVLMPEGQVVISGFNTASLWRLRQIFGFKRESPWDAKFIG
                     LLRLREWLRVLGFELNGGTFGCYAPPFRQRPWLERFAFMEKAGARWWPLLGGLYVVRA
                     VKRVHGMRLVTPAWRQERARRRALAPIPQRSTGNGHARERND"
     gene            2801..3256
                     /gene="rnhA"
                     /locus_tag="VEL04_05580"
     CDS             2801..3256
                     /gene="rnhA"
                     /locus_tag="VEL04_05580"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HYT47870.1"
                     /translation="MTEDLVEIFSDGACRGNPGPGGWGALLRFDKREKELWGGEAATT
                     NNRMELTAVIRALEALERPSRVRLYTDSQYVQKGISAWILDWKRRGWRTAGRKPVKNE
                     DLWRRLDELAALHEVEWHWVKGHAGHTGNERADLLANKGIPDDNKTNSP"
     gene            3231..>3395
                     /locus_tag="VEL04_05585"
     CDS             3231..>3395
                     /locus_tag="VEL04_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HYT47871.1"
                     /translation="MITRQIVLDTETTGLNPKLGDRIIEIGCVEIVDRRISERHFHAY
                     VDPEREIDEGA"
BASE COUNT          551 a         1052 c         1195 g          597 t
ORIGIN      
        1 cggcatgtgc tgcaggctcg ccccttggcc gttcaccggc actacgagca tggtgggcac
       61 cacgcgcacg cgcggcccga tgccgttcac ttccttgagc tggccgagca tcaggccgtg
      121 ctttttcgcg atcgactcga aagtctcgcc cttcttcggg tagtaggtct tccacgacac
      181 gagcgactcg ccctcgagct tgctcaggtt gtcgtggaac acgtcgatct tgtcggcggg
      241 cagcacgatg cgcggcgtca tcgcggcgcg gatcagcggc cggctgaagc cggggttgag
      301 cgccatgaac tcctcgaccg gcatctcggc gagcttggcg gcgagctgga cgtcgatgtc
      361 aaccagcctg gtaaagctgg cgaagtaggg ctggttcggg atcgggtcga gcagcacgcc
      421 gaacggctcg ggattggcga tgatgttctt cagcgcctgg agcttcggca ggtagttgcg
      481 ggtctcggcc ggcatcttga gatgttcgta atcggtcggc ttgccggcgg cgcggttctt
      541 ggcgatggcg cgcgccaccg cgttctcgcc ccagttgtag gaggcgaggg cgagctgcca
      601 gtcgccgtgc atctggtaga ggaaggtaag gtagtcgagc gccgcggtgg tcgaatcgac
      661 gatgtcgcgg cggccgtcgt accaccagtt ctgcttcagc tcgtagcgcc tgccggtgcc
      721 ggggatgaac tgccacaggc ccgaggcatg cgagcgcgaa tacgccatcg ggttgaaggc
      781 gctctcgacc attggcagca gcgccagctc agtcggcagg ctgcgcttct cgatctcctc
      841 gacgatgtga tagaggtaca gccggctgcg gtcgaagacg cgctgcaggt attcggggcg
      901 cgcggcgtaa tacgccgtgt gctgctccac cagcttgttg tcgatgtcgg gtatcgcgaa
      961 gcccctgcgg atgcgctgcc acaggtcccc gggcatcaac gtgaggtcga cttgcggcag
     1021 gcgcgcgcgc tccttgagcg cgcgccgctc gccttcgaac ggcgccgtgg ccgcgggggc
     1081 gggtgctgcg gctaccggtg gattcggcag cggcgcgggc ggcgtgccct ggtcagggat
     1141 cggcgattgc gccgcatgcg gcgaggctcc ctcggacgca ggcccggagg cgcaggcgtg
     1201 cagcagcgcc gccgcgatca gcagggaaaa tgttttgagc agccctttca aaacctgttt
     1261 ttccagtccc ggatcgcggc gaacacgcgt accgggtcgg cgatgcgcgc gccgagatac
     1321 ttgttggccg attcgaccac ggcgggctcg aggcagcgca ggaacgggtt ggtggccttt
     1381 tcttcgcgca gcgtgctcgg cagcgtcggt cttccggact ggcgcagctg ccggtcgcgt
     1441 gactcgcgcg cctggagcgc cgtgttgccg ggctcgactg tcttggcgaa gccgatgttg
     1501 gcgagcgtgt actcgtgccc gcagtagacc ttcgtgtcgt cgggcagcgc agcgagcttt
     1561 ttcagcgacg catacatttg ctccgccgtg ccttcgaaca gccgcccgca gccgcaggcg
     1621 aacagcgtgt cgccacagaa caacgcgcct gccccataat aggcaatgtg cgcacgggta
     1681 tgtccgggaa tgtcgagcac ggagaaggcg acgccgagct gcgcaacgct gaccgtgtcg
     1741 ccctcggata cgggccgggt gagcgtcgcg atcggctcgt ttttcggccc gtagaccgga
     1801 acgcggaatt tttgcagcag ctcggcgatt ccgcctacgt tgtcgggatg gtgatgcgtg
     1861 gcgagaatgg cggcgagctc gagcttctcg cgcgcgagat agtcgagcac cggccgcgcc
     1921 tcgccgggat cgacgacggc ggcatgcgtt tcattgcgca gcgtccagac atagttgtcc
     1981 ttgaacgcgg caaccggaac gatactgagc ggaactcctt ggagcatgta ccggcattcc
     2041 ttcgtgcaaa acggggcgca gaagtataac ggcggacgct catcgctttc agggtggttt
     2101 gccacccccc agggggccta tgtgctcggc tgggaacttg cccagtatga cagcgcagtc
     2161 gatgacgtgt tcggtttcag ggcggtacag atcggcctgc ccgaggtcga ttttctgcgg
     2221 cagaaccgca tccagttccg cttcacgctc gcgctcgagc ccggcgcggc gctcgccgcc
     2281 gatccgctgc agctgccgct cgcgtcgcag agcgtcgacc tgatcgcgct gccgcacgtg
     2341 ctcgagtttc atcccaatcc gcacgaggtg ctgggcgagg ccgagcgcgt gctgatgccg
     2401 gagggacagg tggtcatctc cggcttcaac acggcttctc tctggcgcct caggcagatt
     2461 ttcggtttca aaagagaatc accctgggac gccaagttca tcggcctgct gcggctgcgc
     2521 gagtggctgc gggtgctcgg cttcgagctg aacggcggca cgttcggctg ctacgcgccg
     2581 cctttccggc agcgcccgtg gctcgagcgc ttcgccttca tggaaaaggc cggtgcgcgc
     2641 tggtggccgc tgctcggcgg cctctacgtg gtgcgcgcgg tgaagcgcgt gcacggcatg
     2701 cgcctcgtca cgccggcgtg gcgccaggaa cgcgcgcggc gccgggcgct tgcgccgatt
     2761 ccccagagaa gcacgggcaa tggtcacgcg cgcgagcgca atgactgaag atctggtgga
     2821 gatattcagc gacggagcgt gccgcggcaa cccgggcccc ggcggctggg gggcgctgct
     2881 gcgtttcgac aagagggaaa aggaattgtg ggggggcgaa gcagccacta ccaataaccg
     2941 gatggagctg accgcggtga tccgggcgct cgaggcgctc gaacggccga gccgggtgcg
     3001 gctctacacc gactcgcagt acgtgcagaa gggaatctcg gcctggatcc tcgactggaa
     3061 gcggcgcggc tggcgcacgg ccggcaggaa gccggtgaag aacgaggatc tgtggcggcg
     3121 cctcgacgag ctcgccgcgc tgcacgaggt ggaatggcac tgggtcaagg gccatgccgg
     3181 ccacacgggg aacgagcgcg cggacttgct ggcgaacaag ggtattccag atgataacaa
     3241 gacaaatagt ccttgatacc gaaaccaccg gactcaaccc caagctcggc gaccgcatca
     3301 tcgagatcgg ctgcgtcgag atcgtcgacc gccgcatcag cgagcggcat ttccacgcct
     3361 acgtcgaccc ggagcgcgag atcgacgaag gcgcc
//
LOCUS       DASPXW010000240         2076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1208563, whole genome shotgun sequence.
ACCESSION   DASPXW010000240 DASPXW010000000
VERSION     DASPXW010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1208563"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VEL04_05590"
     CDS             complement(<1..375)
                     /locus_tag="VEL04_05590"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF013391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HYT47872.1"
                     /translation="MCFFQAGRWDAPAGFPAPAFFGSKRGFATAASKSAQHEFLNAAY
                     PLATIAAVFIKGTPMQESPFKGKTGIQRLWNALGYSLQGMVSAFRHEYAFRQECLLAA
                     LLIPAAFFIPVGGAERALMIASV"
     gene            complement(413..1588)
                     /locus_tag="VEL04_05595"
     CDS             complement(413..1588)
                     /locus_tag="VEL04_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HYT47873.1"
                     /translation="MLKRFVPALLMGTCLILTAVPQTGAEPNPGHAGAQASRPIVIGH
                     RGAHGYLPAHTLEGYALAIELGADFIEPDLVATRDGHLIARHEPNMINTTDVRDHPEF
                     ASRRRVAVVDGVPEEGFFASDFTLAEIKRLRAVQDFPERPQQFNGKFEIPTLEEVIAL
                     AKRKSEEKGRTIGIYPETKHPTYHQSIGLPLERRLVAALAEAGWNRRDAPVFIQSFEQ
                     SNLKELRTMTAVRLVQLVDANDVNPDGSLDFTAPFDRPFDWTASGDPRLLARTFGFFA
                     TDEGLREIKTYADGIGPWKRYIVSSVAAGLPGPGEASRTLLPPTDLIERAHKLGLLVH
                     TWTFRNEQRRLVSDYAGNPVNEYLQFYRLGIDGVFSDFADTAVASRILFDLERRDGR"
     gene            complement(1602..>2076)
                     /locus_tag="VEL04_05600"
     CDS             complement(1602..>2076)
                     /locus_tag="VEL04_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HYT47874.1"
                     /translation="TKYIPIIMLTARAQEQDTIAGLEGGADDYVTKPFSPRELLARIK
                     AVMRRRAPQLTDDLVETAGLRLDPVSHRVSAAGENIELGPTEFRMLHFFMTHPERVYS
                     RAQLLDEIWGDHVFVEERTVDVHIRRLRQALEPTKYDSLVETVRGTGYRFRRSLT"
BASE COUNT          348 a          645 c          709 g          374 t
ORIGIN      
        1 cacgctcgcg atcatcaggg ccctttcagc gccgccgacg gggatgaaga aggccgccgg
       61 aatgagcaag gcggcaagca ggcattcctg gcggaaggcg tactcgtgcc tgaacgcgga
      121 gaccatacct tgcagtgaat acccgagcgc gttccacagg cgctgtatcc cggttttgcc
      181 cttgaacgga ctctcttgca tgggtgtgcc cttgatgaag acggcggcga ttgtagcaag
      241 aggatatgcc gcgttaagaa attcgtgttg cgcgcttttt gacgccgccg ttgcgaatcc
      301 tcttttcgag ccgaaaaatg cgggtgcggg aaaaccagcc ggcgcatccc atcgcccagc
      361 ttgaaaaaag cacaaaggcc ggcgcgagcc ggccgtcgct gtcttcggta gatcagcgcc
      421 cgtcgcggcg ctccaggtcg aacaggatgc gcgaggccac cgccgtgtcg gcgaaatccg
      481 agaacacgcc gtcgatcccc agccggtaga actgcaggta ttcgttgacc gggttgccgg
      541 cgtagtccga gacgaggcgc cgctgctcgt tgcggaaggt ccaggtgtgc acgagcaggc
      601 ccagcttatg cgcgcgctcg atcaagtcgg tgggcggcag gagcgtgcgg ctcgcctcgc
      661 ccgggccggg caggccggct gcgaccgagc ttacgatgta gcgcttccag ggaccgatgc
      721 cgtcggcgta ggtcttgatt tcacgcaggc cctcgtcggt cgcgaaaaag ccgaaggtgc
      781 gagcgaggag gcgcggatcg cccgaggccg tccagtcgaa cggccggtcg aagggcgccg
      841 tgaagtcgag gctgccgtcg ggattgacgt cgttcgcgtc caccaactgc accaggcgca
      901 cggcggtcat cgtgcgaagc tccttcaggt tcgattgctc gaaggattgg atgaagaccg
      961 gcgcatcgcg gcggttccat cccgcttcgg cgagcgcggc gaccagccgt cgctccagcg
     1021 gcaggccgat cgactggtgg taggtcggat gcttggtctc cggatagatg ccgatggtgc
     1081 ggcccttctc ctccgatttg cgcttggcga gcgcgatcac ctcctcgagc gtcggaatct
     1141 cgaacttgcc gttgaactgc tgcgggcgct cggggaagtc ctgcaccgcg cgcagccgct
     1201 tgatttccgc gagcgtgaag tcggaggcga aaaaaccctc ctccggcaca ccgtccacga
     1261 cagcgacgcg ccggcgcgat gcgaactccg gatggtccct tacgtcggtg gtgttgatca
     1321 tgttcggctc gtggcgggcg atcaggtgcc cgtcacgggt cgcgacgagg tcgggctcga
     1381 tgaagtccgc gcccagctcg atcgccaggg cgtagccctc cagcgtatgg gcaggcagat
     1441 agccgtgcgc gccgcgatgg ccgattacga tcggcctgct cgcctgtgct cccgcatgac
     1501 cgggattagg ttcggcgccg gtctgcggga cggcagtcag gatcaagcac gtacccatca
     1561 aaagcgcggg gacgaatcgt ttcagcatgg cgatgctcct ttcaggtaag gctgcgccgg
     1621 aagcgatatc cggttccgcg gaccgtttcc acgaggctgt catatttcgt tggctcgagc
     1681 gcctggcgca gcctgcggat gtgcacgtcc accgtgcgct cctcgacgaa gacgtgatcg
     1741 ccccagatct catcgagcag ctgcgcgcgg ctgtagacgc gctccggatg ggtcatgaag
     1801 aagtgcagca tcctgaactc ggtcgggccg agttcgatgt tttcgcccgc ggcgctcacg
     1861 cggtgtgaga ccgggtcgag cctcaagccc gcggtctcga ccagatcgtc cgtgagctgc
     1921 ggcgcgcggc gcctcatcac ggccttgatt cgcgccagca gctcccgcgg cgagaagggc
     1981 ttggtgacgt agtcgtcggc gccgccctcc aggcccgcga tcgtgtcctg ctcctgggcg
     2041 cgcgcggtga gcatgatgat cgggatgtac ttggtg
//
LOCUS       DASPXW010000241         3126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1710215, whole genome shotgun sequence.
ACCESSION   DASPXW010000241 DASPXW010000000
VERSION     DASPXW010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1710215"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..1672
                     /locus_tag="VEL04_05605"
     CDS             53..1672
                     /locus_tag="VEL04_05605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HYT47875.1"
                     /translation="MLPRLKWPLAPGALALLAAAFVLPGLTGHDPWKTHDAVSLGIVH
                     AMAVSGELLVPQVAGAPWMHDPPLYHWIATAFGKLAGQFLEFHAAARLASGALVFAAL
                     YLVYRAGRDWTDDDATRRTTAAAAMLLVLGSIGLMVHAHEAVPELASLAAMCGALAAL
                     PHAARRPLAAGLAFGAGLGLAALSAGWIAPAALAVAVAAALVACPQWRTRAVAGFLGV
                     ALVVAALIAASWPLALAARAPQAFIEWRHLGFRLQEQPLASLRYFLVTASWFAWPAWP
                     LALWAAWSLRRRWQEPRLFVPAAATLAWLAGMVLWGSTQDVNLIPALAPLALLAAPAV
                     FILRRGAAGALDWFGVLTFAFFTAMLWLGYCALVTGMPAPVARNLERIAPGFEHHAAP
                     IPVIVAIALACAWLYLIFFAPHAPLRSLMRWAAGIVLLWGSFAMLALPWVDYQRTYRS
                     VGLQLRAKLPAAAGCIAQKSLGVSQAAALDYHAGIRARAFDASQPAACPLLLVQGSPR
                     HELDGPGPGWTKLADVGRPGDRGERYRLYRK"
     gene            1675..3102
                     /gene="pgi"
                     /locus_tag="VEL04_05610"
     CDS             1675..3102
                     /gene="pgi"
                     /locus_tag="VEL04_05610"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HYT47876.1"
                     /translation="MNTRPPTDCPAWQKLLVHAESWRAAHLRELFAGDAARPAQLTAQ
                     APGLRLDYSRQRLGAMTLRLLAHLAGERGLPEWREALLAGKEINSTEDRAAWHTALRS
                     GENAEVKDVLEKMRRLASRLREERKFRRIVNLGTGGSDLGPRLLVDALGNGKEAGKEA
                     GALEVRFAANADPRDLERALEGAKPDETLFVVVSKSFATQETMTNAAAAKLWGGKTFY
                     AVTSNLEAAKKFGATEILPLWDWVGGRFSVWSAAGFAAMCAIGADSFENFLKGGSEID
                     RHFAEAPLEKNLPVLLALIGVWNTNFLGAATHAVLPYASALRLLPPYLQQLEMESNGK
                     RVDREGKSVEYATAPVLWGMEGTVSQHSFHQLLHQGTQVVPVDFIDISSSEIVSANAR
                     AQADALAFGTEDATLPPHRQYPGNRPSSILFVEELDARNLGRLIALYEHKVFVQGVIW
                     NLNSFDQWGVALGKQLANKILSGGK"
BASE COUNT          453 a         1072 c         1084 g          517 t
ORIGIN      
        1 aagttcaagc ggcgttacac caagaagaag accaccgcct gatccccggg gcatgctgcc
       61 ccgcctgaag tggcccctcg ctcccggcgc gctcgcgctg ctcgcggccg cgttcgtcct
      121 gcccggcctc accgggcacg atccgtggaa gacgcatgac gcggtaagcc tcggcatcgt
      181 ccacgccatg gccgtgagcg gcgagctgct cgtgccgcag gtcgccggcg cgccatggat
      241 gcacgacccg ccgctctacc attggatcgc cacggcgttc ggcaagctcg ccggacagtt
      301 cctggaattt cacgccgccg cgcggctggc gagcggcgcg ctggtgttcg cggcgctcta
      361 cctcgtgtac cgcgcggggc gcgactggac cgacgatgac gcgacgcgcc gcaccaccgc
      421 ggcggcggcg atgctgctgg tcctcggctc gatcggcctc atggtgcacg cccacgaagc
      481 ggtcccggag ctcgcctcgc tcgccgcgat gtgcggcgcg ctcgccgctc tgccgcacgc
      541 ggcgcgccgc cccctggctg cgggcctcgc cttcggcgcg gggcttggac tggcggcgct
      601 ctcggcaggc tggatcgcgc cggcagcgct cgccgtcgct gtggcggcgg ctctggtcgc
      661 ctgcccgcag tggcgcacgc gcgccgtggc gggctttctc ggcgtggcct tggtcgtcgc
      721 cgcgctgatc gccgcgagct ggccgctcgc cctcgcggcg cgcgcgccgc aggcgttcat
      781 cgaatggcgc cacctcggct tccgcctgca ggagcagccg ctcgccagcc tgcgctactt
      841 tctcgtcacc gcgagctggt tcgcctggcc ggcctggccg ctcgcgctat gggccgcctg
      901 gtcgctgcgg cgccgctggc aggagccgcg cttgttcgtt cccgcggccg cgacgctggc
      961 gtggctcgcc gggatggtgc tgtggggatc gacccaggat gtcaatctga taccggcgct
     1021 ggcgccgctc gcgctgctcg ccgcgcccgc ggtattcatc ctgcggcgcg gcgccgccgg
     1081 cgcgctcgac tggttcggcg tgctcacttt cgccttcttc accgcgatgc tgtggctggg
     1141 ctattgcgcg ctggtgaccg gcatgccggc gccggtggcg cgcaacctgg aaagaatcgc
     1201 cccggggttc gagcaccacg ccgctccaat tcccgtgatc gtcgccatcg ccctcgcctg
     1261 cgcctggctg tacctgatct ttttcgcacc gcatgcgccg ctgcgcagcc tgatgcgctg
     1321 ggcggccggc atcgtgctgc tctggggcag cttcgccatg ctggcgctgc cgtgggtcga
     1381 ctatcagaga acctaccgct cggttggcct gcagctgcgc gcgaagctgc ccgccgcagc
     1441 gggttgcatc gcgcagaagt ccctcggcgt atcgcaggcg gcggcgctcg actaccatgc
     1501 cggcattcgc gcccgcgcgt tcgacgccag ccagcctgcg gcctgcccgc tgctcctcgt
     1561 ccaggggagc ccccggcatg agctcgatgg ccccgggccg ggctggacca agctcgccga
     1621 tgtcggccgg cccggcgaca ggggcgagcg ctaccggctg tacaggaagt gagcttgaac
     1681 acccggccgc cgaccgactg tcctgcgtgg caaaaactcc tggtgcatgc cgagagctgg
     1741 cgcgcggcgc atctgcgcga gcttttcgcg ggcgacgctg cgcggccggc acagctcacg
     1801 gcgcaggcgc cgggcctgcg gctcgactat tcgcgccagc gcctgggcgc gatgacgctg
     1861 cggctgctcg cgcatctcgc cggcgagcgc ggcctgccgg aatggcgcga agcgctgctg
     1921 gcaggaaagg agatcaactc gaccgaggat cgcgccgcct ggcacaccgc tttgcgctcc
     1981 ggagaaaatg cagaagtaaa ggacgtgctg gagaagatgc gccgcctggc gtccagactg
     2041 agggaagaaa ggaaattcag gagaatcgtc aatctgggca cgggcggctc ggatctcggg
     2101 ccgcggctgc tcgtcgacgc gctcgggaat ggaaaagaag cagggaaaga agcgggcgcg
     2161 ctcgaggtgc gcttcgccgc caacgccgat ccgcgcgacc tggagcgcgc gctcgaaggc
     2221 gcgaagccgg atgagacgct gttcgtcgtg gtctcgaaga gcttcgccac gcaggagacg
     2281 atgaccaacg ccgcggcggc gaagctttgg ggcggcaaga cgttttacgc cgtgacatcg
     2341 aatctcgagg cggcgaagaa gttcggcgcg actgaaattc ttccgttgtg ggactgggtc
     2401 ggcgggcgct tctcggtgtg gtccgcggcc gggtttgcag cgatgtgcgc catcggcgcc
     2461 gattctttcg agaattttct aaagggagga agtgaaatag accgtcattt cgccgaagcg
     2521 ccgctcgaga agaacctgcc ggtgctgctg gcgctgatcg gcgtgtggaa cacgaatttc
     2581 ctcggcgccg cgacgcatgc cgtgctgccc tacgccagcg cgctgcgcct gctgccgccc
     2641 tatctgcagc agctcgagat ggagtcgaac ggcaagcgcg tcgaccgcga aggcaagagc
     2701 gtcgaatatg ccaccgcgcc cgtgctgtgg gggatggaag gcacggtcag ccagcattcg
     2761 ttccaccagc tgctgcacca ggggacgcag gtggtgccgg tcgacttcat cgacatttct
     2821 tccagcgaga ttgtttcggc caacgcgcgc gcgcaggccg atgcgctcgc tttcggcacc
     2881 gaggatgcga cgctgccgcc gcaccggcaa tatccaggca accgcccgtc gagcatttta
     2941 tttgtcgaag agctcgatgc gcgaaatctc ggccggctga tcgctctcta cgagcacaag
     3001 gtgttcgtgc agggcgtgat ctggaacctc aacagcttcg accagtgggg cgtcgcgctc
     3061 ggcaagcagc tggcgaacaa aattctctcg ggaggcaaat agtggaccag gaaaccttca
     3121 acctca
//
LOCUS       DASPXW010000242         2669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_198663, whole genome shotgun sequence.
ACCESSION   DASPXW010000242 DASPXW010000000
VERSION     DASPXW010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198663"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1126
                     /locus_tag="VEL04_05615"
     CDS             <1..1126
                     /locus_tag="VEL04_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HYT47877.1"
                     /translation="VFTQSGEAGGARFWRGTASAPDLYNLLTRFLGGAGADEAFRDYA
                     AAKGLRWPDARLDADAELVHYVEVQLAGAIGAASARVMVASVVKEEALSIDEVREIVD
                     EASQLVVYSHRLEQKSRELEAATGELRAANERLKELDRLKDDFVSTVSHELRTPLTSI
                     RAFTQILFEAPDVELEQRKKFLGIITKETERLTRLINQVLDLSKIESGRAEWQASRLD
                     LREVIADTVTGMSQVFEERSIKVELKLPERVAAINADVDRIIQVLLNLLSNAAKFCDP
                     AEGRIEIGLSQHGGSLRVDVRDNGAGIEPAYQGVIFDKFRQVGDTLTAKPHGSGLGLH
                     ISRQIVEHYGGRMWVESRPGGGACFSFTLPLDVAAASRAA"
     gene            1123..1491
                     /locus_tag="VEL04_05620"
     CDS             1123..1491
                     /locus_tag="VEL04_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYT47878.1"
                     /translation="MSKKILVADDEPNILTALEFLLQRNGYDVQIARNGEEALQLIES
                     GPPDLVLLDVMMPLRSGYEVCRRIRERTDWSHIKIVMLSAKGRDAEVTKGLAMGADLY
                     VTKPFSTRELVDKVKALLPP"
     gene            1624..1887
                     /locus_tag="VEL04_05625"
     CDS             1624..1887
                     /locus_tag="VEL04_05625"
                     /inference="COORDINATES: protein motif:HMM:TIGR03647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HYT47879.1"
                     /translation="MSELTAAQRDAYWRKTSRLMWIIMFLWVVFSFVIPAFAVQLNEI
                     KIFGFPLGFYMAAQGSLIFYVVVCFWNASAQNKIDKEFDVAED"
     gene            1904..>2669
                     /locus_tag="VEL04_05630"
     CDS             1904..>2669
                     /locus_tag="VEL04_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VC_2705 family sodium/solute symporter"
                     /protein_id="HYT47880.1"
                     /translation="MANQTRTSFVDNLGKIYGVYAGGFIGFVILLAILEQMGVPNRVI
                     GYCFVFLTIGVYAYIGVISRTSEISEYYVAGRVVPPFFNGMATGADWMSAASFISMAG
                     GLYLAGYGGLGYVLGWTGGYVLVGVLLAPYLRKFGQFTVPDFLGVRYEGNTARMCGVV
                     VLFCASFTYIVAQCFGIGIITSRFLDIPFEIAIFVGLLGILVCSMLGGMRAVTWTQVA
                     QYIILIIAYLIPVVWLSIKLFGNPFPELAYGQVLDQI"
BASE COUNT          459 a          840 c          878 g          492 t
ORIGIN      
        1 tgtcttcacg cagagcggcg aggcgggcgg cgcgcgcttc tggcgcggca cggcgtcggc
       61 gccggacctg tacaacctgc tgacgcgctt tctgggcggc gcgggggcgg acgaggcatt
      121 tcgcgactac gcggcggcaa agggcctgcg ctggcctgac gcgcgcctcg atgccgatgc
      181 cgagctggtg cactacgtcg aagtgcagct cgcgggggcg atcggcgccg cttcggcgcg
      241 ggtcatggtg gcctcggtgg tgaaggagga ggcgctcagc atcgatgaag tgcgcgagat
      301 cgtcgacgag gcctcgcagc tcgtggtcta cagccaccgg ctcgagcaga agtcgcgcga
      361 gctcgaggcg gcgaccggcg agctgcgcgc cgcgaacgag cgcctgaagg agctcgaccg
      421 gctgaaggac gatttcgtct cgaccgtgag ccacgagctg cgcacgccgc tcacttcgat
      481 ccgcgcgttt acccagatcc tgttcgaggc cccggacgtc gagctcgagc agcgcaaaaa
      541 attcctcggc atcatcacca aggaaacgga gcggctgacg cggctcatca accaggtgct
      601 cgacctctcg aagatagaat ccggcagggc ggaatggcag gcgtcgcggc tcgacctgcg
      661 cgaggtgatc gccgatactg tcaccggaat gagccaggtg ttcgaggagc gcagcatcaa
      721 ggtcgagctg aagctgccgg agcgggttgc cgcgatcaac gccgacgtcg accggatcat
      781 ccaggtgctg ctgaacctgc tgtcgaatgc cgccaagttc tgcgacccgg ccgagggccg
      841 catcgagatc gggctttcgc agcacggcgg gtcgctgcgg gtcgacgtgc gggacaacgg
      901 cgccggcatc gagcccgcct accagggcgt catcttcgac aagttccgcc aggtcggcga
      961 cacgctcacc gcaaagccgc acggcagcgg cctcgggctg cacatcagcc gccagatcgt
     1021 cgagcattac ggcggccgga tgtgggtcga gagccggccc ggcggcggag cgtgcttctc
     1081 gttcacgctg ccgctcgacg tcgctgccgc ctcgcgcgcc gcatgagcaa gaaaatcctg
     1141 gtcgccgacg acgagccgaa catcctcacg gcgctcgagt tcctcctgca gcgcaatggg
     1201 tacgacgtgc agatcgcgcg caatggcgag gaggcattgc agctgatcga gagcggcccg
     1261 cccgacctgg tcctgctcga cgtgatgatg ccgctgcgca gcggctacga ggtgtgcagg
     1321 cgcatccgcg agcgcacgga ctggagccac atcaagatcg tcatgctgtc ggcgaaaggg
     1381 cgcgacgccg aggtcaccaa gggcctggcg atgggcgccg atctctatgt caccaaaccg
     1441 ttctccaccc gcgagctggt cgacaaagtc aaggcgctgc tgccgccgta agcagagcaa
     1501 gaaccgtttg tgcggcgcgg caatcacatt cctatttgcg taatcgtaag tttggtgtaa
     1561 gcatccgcgt ccaagaatct aggggaaacc ctagtcggtt cctttcttgg tcgaggaggc
     1621 aagatgagtg aattgacggc cgcgcaacgc gacgcgtact ggcggaaaac gtcccgcctg
     1681 atgtggatca tcatgttcct gtgggtggtt ttcagcttcg tgatacccgc attcgccgtg
     1741 cagctgaacg agatcaagat tttcggcttc ccgctcggtt tctacatggc ggcgcagggc
     1801 tcgctgatct tctacgtggt ggtgtgcttc tggaacgcga gcgcgcagaa caagatcgac
     1861 aaagaattcg acgtagccga agactgaccg ggagcgacca cccatggcca accagaccag
     1921 aacgagcttc gtcgacaacc tcggcaagat ctacggcgtc tacgccggcg gttttatcgg
     1981 ctttgtcatc ctcctcgcga tcctggagca gatgggggtg ccgaacaggg tcatcgggta
     2041 ctgcttcgtg ttcctgacca tcggcgtgta cgcctacatc ggcgtcatct cgcgcacttc
     2101 cgagatttcc gagtactacg tcgcgggccg cgtggtgccg ccgttcttca acggcatggc
     2161 gaccggcgcc gactggatga gcgcggcctc gttcatctcg atggccggcg gcctgtacct
     2221 ggccggctat ggcggcctcg gctacgtgct gggctggacc ggcggctacg tgctggtcgg
     2281 cgtcctgctc gcgccctacc tgcgcaagtt cggccagttc acggtgcccg atttcctcgg
     2341 cgtgcgctac gagggcaaca ccgcgcgcat gtgcggcgtg gtggtgctgt tctgcgcgtc
     2401 cttcacctac atcgttgccc agtgcttcgg catcggcatc atcacctcgc gctttctcga
     2461 cattccgttc gagatcgcga tcttcgtcgg cctgctcggc atcctggtgt gctcgatgct
     2521 cggcggcatg cgcgcggtga cctggaccca ggtcgcgcaa tacatcatcc tgatcatcgc
     2581 ctacctgatt ccggttgtct ggctgtcgat caagctcttc ggtaatccgt tcccggaact
     2641 cgcctatgga caggtgctgg atcagatcg
//
LOCUS       DASPXW010000243         4096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1209831, whole genome shotgun sequence.
ACCESSION   DASPXW010000243 DASPXW010000000
VERSION     DASPXW010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1209831"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VEL04_05635"
     CDS             <1..577
                     /locus_tag="VEL04_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HYT47881.1"
                     /translation="SGLNGFNAGQFSFVLFNETLSRVLSLEVSAMEADGKGKIIASPR
                     VLTADQVEAVIEQGTEIPYQQATSSGATAVSFRKANLSLKVKPQITPDGSIIMTLDVN
                     KDQPGAATAAGLQINTKHVKTEVLVENGGTVVIGGIYEQQDRTDITKIPFFGDLPFLG
                     VLFRATITSSNKTELLVFITPRIVNERLTIR"
     gene            660..1193
                     /locus_tag="VEL04_05640"
     CDS             660..1193
                     /locus_tag="VEL04_05640"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HYT47882.1"
                     /translation="MKRAGNLYLVGLMGAGKTTVGRLLARRLKLRFLDSDHEIEQRCG
                     VKVPVIFEIEGEAGFRSRESQAIAELTALEGIVLATGGGVVISSENRRLLAASGTVVY
                     LCARPEDLYERVRHDRNRPLLATPDPLARLRELYAERDPLYRALADLVVDTGRQGVQA
                     LARDLLEKLEERWKASA"
     gene            1175..2257
                     /gene="aroB"
                     /locus_tag="VEL04_05645"
     CDS             1175..2257
                     /gene="aroB"
                     /locus_tag="VEL04_05645"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HYT47883.1"
                     /translation="MESLSVTLGERSYPIHVGAGLLDLSELYRPHLAGGSAALITNEV
                     VAPLYLKRISKTLAAAGARVTEIVVEDGEQAKRWQTLERVFDQLLAAHCGRDTLIVAL
                     GGGVVGDLAGFAAAVYQRGVAVLQVPTTLLAQVDSSVGGKTAINHARGKNMIGAFHQP
                     RAVISDVATLDTLPERELRAGLAEVIKHGVALDAEFTQWLEGNLERLLARERASVIHA
                     VRRCCELKARVVAADEREAGERALLNFGHTFGHAIEAGTGYGTWLHGEAIAAGMVMGA
                     ELSMRLGLIGKNEVARLRKLIARAGLPVNGPALAPEQLLALMSVDKKAAKGHVRFVLL
                     EAIGKAALRGGVDERQVREAIVAAAQ"
     gene            2280..>4096
                     /locus_tag="VEL04_05650"
     CDS             2280..>4096
                     /locus_tag="VEL04_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011402558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HYT47884.1"
                     /translation="MSLQAQGLYHPANEHDACGVGFVAHIKGRKSHTIVEQGLTVLRN
                     LTHRGAVGWDPKLSDGAGCLIQIPDRFFREEMARQGVKLPPAGQYGVGMVFLPRDPAS
                     RIACEYEIERAIKDEGQALLGWRDVPVDNSDLAEPARNIEPVIRQVFIGRGRRVTVTD
                     ALERKLYIIRKSAGHAIQALKLEHGKEFYVPTMSARTICYKGMLLAYQVGEYYLDLKN
                     PRVESALALVHQRFSTNTFPSWDLAHPFRMICHNGEINTLRGNVNWIRARQGAISSPV
                     LGEDLDKIWPLIYDGQSDSASFDNALELLVMSGYSVAHAVMMMIPEAWENHSLMDPAR
                     RAFYEYHAAMMEPWDGPASIAFTDGRLIGATLDRNGLRPSRYIVTADDLVIMGSECGC
                     LPVPEERIIKKWRLQPGKMFLVDLEKGRIIDDKELKDALAGARPYAEWIERIRVKLDE
                     VESEKSVPFKSEVPLLDRQQAFAYTQEDIKFQLQPMAQAGEEPVGSMGNDASLAVLSR
                     RNKTLYHYFQQLFAQVTNPAIDPIREELVTSLVSFIGPKPNLLGIDEMNPPLRLEVSQ
                     PVLDFYEMEKVRYIERYTGGKFKSLELDITYPAAWGKEA"
BASE COUNT          730 a         1310 c         1375 g          681 t
ORIGIN      
        1 gtctggcctc aacgggttca atgccggcca gttttcgttc gtgctcttca acgagaccct
       61 gtcgcgggtg ctgagcctcg aggtttcggc gatggaggcc gacggcaagg gcaagatcat
      121 cgccagcccg cgggtgctca cggcggacca ggtggaagcc gtgatcgagc agggcacgga
      181 gatcccctac cagcaggcca cttcgtcggg agcgaccgcg gtctccttcc gcaaggccaa
      241 tctctcgctc aaggtgaagc cgcagatcac ccccgacggc agcatcatca tgacgctcga
      301 cgtgaacaag gatcagcctg gcgccgccac ggcggcaggc ctgcagatca ataccaagca
      361 cgtcaagacc gaggtgctgg tcgagaacgg cggcacggtg gtgatcggcg gcatctacga
      421 gcagcaggac cgcaccgaca ttaccaagat cccgtttttc ggcgacctgc cgttcctcgg
      481 cgtcctgttc cgcgccacca tcacctcgtc caataaaacc gagctgctgg tattcattac
      541 gccgcgcatc gtcaatgagc ggctgactat tcgctgatcg ccgcgtctcc tgccgttgct
      601 gttctggacg gtacgacggc cgggtccccc cggccgtttt tgtttctaca atgccgggga
      661 tgaagcgcgc gggtaacctg tacctggtgg ggctgatggg cgccggcaag acgaccgtcg
      721 ggcgcctgct cgcgcggcgc ctgaagttgc gcttcctgga ctccgaccac gagatcgagc
      781 agcgctgcgg ggtgaaggtg ccggtgattt tcgagatcga aggcgaggcc ggctttcgca
      841 gccgcgagtc gcaggcgatc gccgagctca ccgcactcga gggcatcgtg ctcgcgaccg
      901 gcgggggcgt ggttatttcg agcgagaatc gtcggctgct ggcggcaagc ggcaccgtgg
      961 tgtacctgtg cgcgcggccg gaggatctct acgagcgcgt gcgccacgat cgcaaccggc
     1021 cgctgctcgc gacccccgac ccgctcgcgc gcctgcgcga gctctatgcc gagcgcgatc
     1081 cgctgtaccg tgcgctcgcc gatctggtcg tcgacaccgg caggcaaggc gtgcaagcac
     1141 tggcgcgcga tctgctggag aagctcgagg agcgatggaa agcctcagcg taacgctcgg
     1201 cgagcgctcc tatccgatcc acgtcggggc gggattgctg gatctttccg agctgtatcg
     1261 cccgcacctg gcaggcggca gcgcggcgct gatcaccaat gaggtcgtcg ctccgctgta
     1321 cctcaaaaga atctcaaaaa cgcttgcagc agcgggcgcg cgggtgaccg agatcgtggt
     1381 cgaggacggc gagcaggcga aacgctggca gaccctcgag cgcgtattcg accagctgct
     1441 cgcggcgcat tgcggtcgcg atacgctgat tgtcgcgctc ggcggcggag tggtgggcga
     1501 tctcgccgga ttcgccgccg cggtgtatca gcgcggcgtg gccgtgctgc aagtacccac
     1561 cacgctgctc gcccaggtcg attcctcggt cggcggcaag accgccatca accatgcgcg
     1621 cggcaagaat atgatcggcg ccttccacca gccgcgcgcg gtgatctcgg acgtggccac
     1681 gctcgacacg ctgcccgagc gcgagctgcg cgcagggctc gcggaggtga tcaagcacgg
     1741 cgtcgccttg gacgccgagt tcacgcagtg gctggagggc aacctcgaaa gactgctcgc
     1801 acgcgagcgc gccagcgtga tccatgcggt acgccgctgc tgcgagctga aagctcgcgt
     1861 cgtcgccgcc gacgagcgcg aagcgggcga gcgcgcgctg ctcaatttcg gccacacctt
     1921 cggccatgcg atcgaagccg gcaccggcta cggcacctgg ctgcacggcg aggcgatcgc
     1981 ggcgggcatg gtcatggggg ccgagctctc gatgcgcctg ggtttgatcg gaaaaaatga
     2041 agtcgcaagg ctgcggaagc tgattgcacg cgcgggattg ccggtgaacg ggcctgcgct
     2101 cgcgccggag cagctgctgg cgctgatgtc ggtcgacaag aaagccgcaa agggccacgt
     2161 gagattcgtg ctgctggagg ccatcggcaa ggcggcgctg cgcggcggag tcgacgagcg
     2221 ccaggtccgc gaagccattg tcgctgcagc gcaataattg tatattcaat agttcggtca
     2281 tgagtttgca ggcacagggg ctctatcacc cggccaacga gcacgacgct tgtggcgtcg
     2341 gcttcgtcgc gcacatcaag ggccgcaagt cacacaccat cgtcgagcag ggcttgacgg
     2401 tgctgagaaa tctcacccat cgtggcgccg tcggctggga tccgaagctt tccgatggcg
     2461 ccggctgcct gatccagatc cccgatcgct tcttccgcga ggagatggcg cgccagggcg
     2521 tgaagctgcc gcctgcgggc cagtacggag tcggcatggt gttcctgccg cgcgatccgg
     2581 cctcgcgcat cgcctgcgag tacgagatcg agcgcgccat caaggacgag ggccaggcgc
     2641 tgctcggctg gcgcgacgtg ccggttgaca actcggacct ggccgagccg gcgagaaaca
     2701 tcgagccggt gatccgccag gtattcatcg gtcgcggccg gcgcgtcacg gtcaccgacg
     2761 cgctcgagcg caagctctac atcatccgca agagcgccgg ccacgccatc caggcgctca
     2821 agctcgagca cggcaaggag ttctatgtgc cgacgatgtc ggcgcgcacc atctgctaca
     2881 agggaatgct gctcgcctat caggtcggcg agtactacct cgacctgaag aacccgcgcg
     2941 tcgagtcagc gttggcgctc gtgcaccagc gtttttccac caacaccttc ccgtcgtggg
     3001 acctggcgca cccgttccgc atgatctgcc acaacggcga gatcaacacg ctgcgcggca
     3061 acgtcaactg gatccgcgcg cggcaggggg cgatctcgag cccggtgctc ggcgaggatc
     3121 tcgataagat ctggccgctg atttacgacg gccagtcgga ttcggcctcg ttcgacaacg
     3181 cgctcgagct tctggtgatg agcggctact cggtggcgca cgccgtgatg atgatgattc
     3241 cggaggcgtg ggaaaaccac agcctgatgg atccggcgcg gcgcgcgttc tacgagtacc
     3301 acgcggcaat gatggagccg tgggacgggc cggcgtcgat cgcgttcacc gacggtcgcc
     3361 tgatcggcgc cacgctcgac cgcaacggcc tgcggccatc gcgctacatc gtcaccgccg
     3421 acgacctcgt gatcatgggc tcggagtgcg gctgcctgcc ggtgcctgag gagcgaatca
     3481 tcaagaagtg gcgcttgcag ccgggcaaga tgttcctggt cgatctcgag aagggccgca
     3541 tcatcgacga caaggagctg aaggacgcgc tcgccggcgc gaggccctac gcggaatgga
     3601 tcgagcgcat ccgcgtcaag ctcgacgagg tggagtcgga gaagagcgtg ccgttcaagt
     3661 ccgaggtgcc gctgcttgac cggcagcagg ctttcgccta cacgcaggag gacatcaagt
     3721 tccagctcca gcccatggcc caggcgggcg aggagccggt tggctcgatg ggcaacgacg
     3781 cttcgctggc ggtgctctcg aggcggaaca agacgctcta ccattatttc cagcagctct
     3841 tcgcgcaggt cacgaatccc gcgattgacc cgattcgcga ggagctggtg acctcgctcg
     3901 tcagcttcat cgggccgaag ccgaacctgc tcggcatcga cgagatgaac ccgccgctgc
     3961 ggctcgaagt gagccagccg gtgctcgatt tctacgagat ggagaaagtg cgctacatcg
     4021 agcgctacac cggcggcaag ttcaagtcgc tcgaactcga catcacctac ccggcggcct
     4081 ggggcaagga ggcgat
//
LOCUS       DASPXW010000244         2654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_953989, whole genome shotgun sequence.
ACCESSION   DASPXW010000244 DASPXW010000000
VERSION     DASPXW010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953989"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VEL04_05655"
     CDS             complement(<1..198)
                     /locus_tag="VEL04_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYT47885.1"
                     /translation="MAILWTGSTRSAELRIGLAADVTSMDPHFLNIAPNINIAWHVFD
                     ALTHVDEDARLIPGLAVSWRAI"
     gene            complement(284..2194)
                     /locus_tag="VEL04_05660"
     CDS             complement(284..2194)
                     /locus_tag="VEL04_05660"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYT47886.1"
                     /translation="MYLLRGVFAARKAKRRLADAHRFFEAGVLEDAEQACDAVADVSP
                     DLADVHYLRGRIELQRQRLELAVGHLEAAVRRRDSEASFHLTLAETFQKLGRHDRAAT
                     HFQRALECLAPPGSGRIAVMLQLAAALQASNDFDGAEKWHRKVLEIEPYNREALLRLA
                     MQRFEESDAAEARSLMNRYIASPADAAARLRRALILTAIVQSNEQIEAERKRFEQELE
                     ELLGSRLAPVSRPEREVGTTAFYLAYHGRDNSALLRRLAQVCRSLYPARSRCERKLFA
                     SGRRLRIGFVSTFFHFHSVGRTTLGLIRDLPRDRFEVSVFAISPQPDELADAIRRNAD
                     HYVALPEDLERVRETIDAAALDMVLFADIGMHPLTYFLALWRLAPIQLTTWGHSVTSG
                     IDTIDYYVSSDCVEPEESEGLYTEKLIRLPGYFMPRYPRPALEGPRRTRRELGLPEDA
                     HVYYCPQNLFKLHPDFDATLRAILERDPQGGILLIESRKSWADLIRRRFARTLGAVAS
                     RVHFVGRVPYPEYLQYVAAADVILDPFYFGGCNSSCEALSLAVPVVTLPSFQLPGRFT
                     MGLYEEIELRDCVARGPEQFVEIAVRLGMDGDYRRAISAQIAARCERLFDRPDTGLAL
                     GDELLRIAETAR"
     gene            complement(2247..>2654)
                     /locus_tag="VEL04_05665"
     CDS             complement(2247..>2654)
                     /locus_tag="VEL04_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HYT47887.1"
                     /translation="RGRGAYLNERRMRVSRRAQIGDALLGTGFPFRDLQGLEEYLAMF
                     RALTVCTAGIRRAGAASLDLAYVAAGRLDGFWEIGLAPWDMAAGALLILEAGGLVGDL
                     QGESGWLESGRIACGNPKIFVQMLRAIAPHLKA"
BASE COUNT          475 a          874 c          883 g          422 t
ORIGIN      
        1 gatcgcacgc caagagacgg cgaggcccgg aataaggcgc gcgtcctcgt ccacgtgggt
       61 gagcgcgtcg aacacgtgcc aggcgatatt gatgttcggc gcgatgttga ggaagtgcgg
      121 atccatcgag gtgacatccg cggcgaggcc gatgcgcagc tcggcggacc gcgtggatcc
      181 cgtccacaga atcgccaccg caagcgaaat tcggatcagt cggcacaccg cgcttttgca
      241 ttcggcttcg cgcacggggt ggcccttgcc cgccttgcct tggtcagcga gcggtctcgg
      301 caatgcgcag cagttcgtcg cccagggcga ggcccgtgtc gggacgatcg aacaaccgtt
      361 cgcatcgcgc tgcgatctgc gccgatatcg ccctgcgata atcgccgtcc atgccgagcc
      421 tcaccgcgat ctcgacgaac tgctccggcc cccgagcgac gcagtcccgc agctcgatct
      481 cctcgtagag tcccatcgtg aaccgccccg gcagctgaaa cgagggcaga gtcacgacgg
      541 gaacggcgag ggaaagcgcc tcgcagctcg aattgcaccc tccgaagtag aacgggtcga
      601 ggatgacgtc cgctgcggca acatactgca ggtattccgg ataaggcacg cgcccgacga
      661 aatgcactcg cgacgcgacc gcgccaaggg tccgcgcaaa gcgcctgcga atgagatcgg
      721 cccagctctt gcgggattcg atcagcagga ttccgccctg aggatcgcgc tcgagaatcg
      781 ccctgagggt ggcatcgaag tccggatgca gcttgaacaa gttctgcggg cagtagtaga
      841 cgtgggcgtc ctcgggcaga ccgagctccc tgcgcgtcct gcgcggtccc tccagcgcgg
      901 gcctcgggta gcgcggcatg aaatatcctg gcaggcgaat cagcttctcg gtgtacagac
      961 cctcgctttc ctccggttcg acgcagtccg aggacacgta atagtcgatc gtgtcgatgc
     1021 cgctggtgac cgaatgcccc caggtcgtaa gctggatggg cgcgagccgc cacagcgcca
     1081 ggaagtaggt caggggatgc atgccgatgt cggcgaaaag gaccatgtcg agcgcggcgg
     1141 cgtcgatcgt ttcgcgaacg cgctccagat cctcggggag ggcgacgtaa tggtcggcat
     1201 tgcgccggat cgcgtccgcc agttcgtccg gctgcgggct tatggcgaag acggatacct
     1261 cgaaccggtc gcggggcaga tcgcgaatga gacccagggt ggtgcggccg acggaatgga
     1321 aatggaaaaa agtggaaacg aacccgatcc gcagacggcg tcccgaggcg aacagcttgc
     1381 gctcgcagcg gcttcttgcc ggatacaacg agcgacaaac ctgcgcgaga cggcgaagca
     1441 gcgcgctgtt gtcgcggcca tgatacgcga ggtagaacgc cgtcgttccg acttcgcgtt
     1501 ccggtcggct gaccggcgcg aggcgtgagc cgagcaattc ttcgagctcc tgctcgaacc
     1561 gttttcgctc cgcctcgatc tgctcgttcg attgcacgat ggcggtcaaa atcagcgcgc
     1621 gcctcagccg cgcggcggca tcggccggcg acgcgatgta tcgattcatc aagctgcgcg
     1681 cctccgcggc gtcggactcc tcgaaccgct gcatcgcgag gcgcaagagc gcctcgcggt
     1741 tgtagggctc tatttcgaga accttgcggt gccatttttc cgcgccatcg aagtcgttgc
     1801 ttgcctggag cgcggccgcc agctggagca tcaccgcgat cctcccggac ccgggcgggg
     1861 caagacactc cagcgcgcgc tggaaatgag tggcggcccg gtcgtggcgg cccagtttct
     1921 ggaaggtttc cgccagggtc aggtgaaagg aggcttccga atcgcgccgc cgcacggccg
     1981 cctccagatg ccccacggcc agctcgagcc gctgccgctg gagctcgatc cgtccgcgca
     2041 ggtagtgtac gtcggcaaga tcgggcgaaa catcggcaac cgcatcgcag gcctgctccg
     2101 cgtcctcgag cacgccggcc tcgaaaaacc ggtgcgcgtc ggcgaggcgc cgtttcgctt
     2161 tgcgcgcggc gaagacgccg cggagcaagt acatcagcat cggtacgatg tctcctggcg
     2221 cctgggtagc cggcctgtct cggcgctcaa gccttgaggt gcggggcgat cgcccgcagc
     2281 atttgcacga agatcttggg attcccgcac gcgatgcggc ccgattcgag ccagcccgac
     2341 tcgccctgca ggtcgccgac cagccccccg gcttcgagga tgagcagcgc ccccgccgcc
     2401 atgtcccagg gagccaagcc gatctcccag aagccgtcga gcctgccggc agccacgtag
     2461 gcgagatcga gcgacgccgc cccggcgcgg cggatccctg cggtgcacac cgtcagcgcg
     2521 cggaacatcg cgagatactc ctccaggccc tgcagatcgc ggaaagggaa tccggtgccg
     2581 agcagggcgt cgccgatctg tgcccgcctg gaaacgcgca tgcgccgctc gttcagatag
     2641 gcgccgcgtc cgcg
//
LOCUS       DASPXW010000245         4008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_451046, whole genome shotgun sequence.
ACCESSION   DASPXW010000245 DASPXW010000000
VERSION     DASPXW010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451046"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VEL04_05670"
     CDS             complement(<1..221)
                     /locus_tag="VEL04_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006396843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HYT47888.1"
                     /translation="MAGPDSWFIVAAVTGTFLLAGFVKGVIGLGLPTLAMGLLGIAMP
                     PAQAASLLVMPSLITNFWQLAAGPSLLAL"
     gene            complement(241..585)
                     /locus_tag="VEL04_05675"
     CDS             complement(241..585)
                     /locus_tag="VEL04_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47889.1"
                     /translation="MRPGPIAALAAALAACTPMLWSKQDASAEQLEQDQRGCEEQAYR
                     EATARSWGYHAIGPAVFQDSLGRRFNVYPSGPFADQFGNQLMEESRLTSACMRSKGYE
                     LVPAPKKKSNPA"
     gene            689..1780
                     /locus_tag="VEL04_05680"
     CDS             689..1780
                     /locus_tag="VEL04_05680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HYT47890.1"
                     /translation="MPTATLLDAPLAIVDLETTGTRPGIDRVTEIAVLEVDGFALTAK
                     WSTLVNPGVGIPPEIQALTGITREMVEVAPRFAELAEELYAQLAGRVFVAHNARFDYG
                     FLRREFDRAGLKFRARTVCSVKLARRLYPGMRHDLDSLIERHALDCRARHRALGDAEA
                     LWQFLQLAAREHGEEILAVAARQVARQAALPPQLDRSMVDAIPEAPGVYLFYGEGPAP
                     LYVGHGKTLRSRVLEHLSSKPPRLARRVRRIEWQRTAGELGARLREASLVKQLDPAFN
                     RHLRRPRQFEPADWPHRGPLGIVETDHARDATEVHVVDRWCYLGTASSDPEVAELLET
                     REPRFDYNHYRILARHLGRRGVRVVPLGY"
     gene            1780..2757
                     /locus_tag="VEL04_05685"
     CDS             1780..2757
                     /locus_tag="VEL04_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47891.1"
                     /translation="MHCELLVPGLFSAGAQARLPALEMLLARGRSQAAPSQRLEGWLQ
                     QAFDLEGQPLAAGALTLLGSGGDPGAARWARADPVHLRLMRDRLILVPAAALEISRQE
                     AEALCAALSAHFAGALAFRAVDPRRWCVRLEKELALGEVAPLEMAGREVLLAGAGDAL
                     LNEVQMLLHAHPVNEARETRGEPPLNSLWLWGAGRVPREARCRWHSISSDDPLALGLA
                     RLAGARHRGLPASAGAWLDRAPEEGRHLVILDALRAPLALSQEAEYRAGVETLERGWF
                     APLLGALREGRVGMVTLQVPDAAAGASFETIRGDLRRFWRRPKALERYA"
     gene            2754..>4008
                     /locus_tag="VEL04_05690"
     CDS             2754..>4008
                     /locus_tag="VEL04_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HYT47892.1"
                     /translation="MKIVERPYRESDRRALLEAGVHPILARVYAGRRIGSARELRYDP
                     ANLLAPASLRNAQNAAQLLAAAIEGGKRLLIVADYDADGATACAVGMRALRAFGATVD
                     YLVPDRFKLGYGLSPELVEIAAERKPDVLITVDNGIASVEGVARASELGIATLITDHH
                     LPGAELPAAACIVNPNQPGCDFASKALAGVGVMFYVMLALRAELRTRGKENAEINLGA
                     LTDLVALGTIADVVPLDANNRTLVAQGLKRLRAGKAQPGIAALLRAAGRAPTEASSFD
                     LGFVVGPRLNAAGRLADMGLGIECLLTDDEARAANCAQALDRLNRERRKIEAGMLEQA
                     MQELKKLDGKSRPAFFSPHWHEGVVGILASRLKDRLHHPVICFARSAQGGWLKGSGRS
                     IPGLHLRDCLDLVSKRAPGLLLRFGG"
BASE COUNT          594 a         1400 c         1390 g          624 t
ORIGIN      
        1 atcagcgcga gcaagctcgg gccggcggcg agctgccaga agtttgtaat gagcgacggc
       61 atgacgagca gcgacgcggc ttgcgccggc ggcatggcga ttccaagcag tcccatcgcg
      121 agcgtgggaa gaccgagccc gatgactccc ttgacgaaac ctgcgagcaa aaacgtgccg
      181 gtgaccgctg cgacaatgaa ccaggaatcg gggccggcca tccgacgcag cgattttggc
      241 ctaggcggga ttactttttt tcttcggtgc cggaaccagc tcgtagccct tgctgcgcat
      301 gcaggcgctg gtgaggcgcg attcttccat cagctggttg ccgaactggt cggcgaacgg
      361 tcccgaaggg tagacgttga agcggcggcc gagcgagtcc tggaacacgg ccgggccgat
      421 cgcgtggtag ccccaggagc gcgcagtcgc ttcgcggtag gcctgctctt cgcagccgcg
      481 ctggtcctgc tcgagctgct cggcgctcgc gtcctgcttc gaccacagca tcggcgtgca
      541 cgcggccagc gcagccgcaa gggcggcaat cggtcccggt ctcatccagc gttggacagc
      601 taaagtcagc gcgatgctca cggtatacgg gaccgggctg ctcctcaagg gtttttgcgc
      661 tagagtcgct actggacgga tatccagctt gcctaccgcc acgctgctcg atgcaccgct
      721 cgcgatcgtc gatctggaga cgaccggcac gcggccgggc atcgaccgcg tcacggagat
      781 cgcagtgctc gaggtggacg gcttcgcgct gacggcgaaa tggtcgaccc tggtgaatcc
      841 aggcgtcgga attcccccag aaatccaggc gcttaccggg ataacgcgcg agatggtgga
      901 ggtcgcgccg cgcttcgccg agctcgccga ggagctctac gcgcagctcg caggccgggt
      961 attcgtcgcg cacaacgcgc gcttcgacta tgggttcttg cgccgcgaat tcgaccgcgc
     1021 ggggctcaag ttccgcgcca ggaccgtgtg cagcgtcaag cttgcgcggc gcctgtatcc
     1081 gggcatgcgc cacgatctcg acagcctgat cgagcgccac gcccttgatt gtcgcgcgcg
     1141 gcaccgggcg ctcggcgacg ccgaggcgct gtggcaattc cttcagctcg ccgcgcgcga
     1201 gcacggcgag gagatcctcg ccgtcgcggc gcgccaggtc gcccggcaag cggcgctgcc
     1261 gccgcagctc gaccgttcca tggtcgacgc gatccccgag gcccccggcg tttatctttt
     1321 ctatggcgaa ggccctgcgc cgctttacgt tggtcatggc aagactcttc gctcgcgcgt
     1381 gctcgagcac ttgtcgtcaa aaccaccccg attggcacgc cgagtgcggc gcatcgaatg
     1441 gcagcgcacc gcgggcgagc tcggcgcgcg gttgcgcgag gcaagcctgg tcaagcagct
     1501 cgacccggcg ttcaaccggc acctgcgccg gccgcggcag ttcgagcctg ccgactggcc
     1561 gcaccgcggc ccgctcggca tcgtggaaac ggaccacgcg cgagatgcca cagaggtgca
     1621 cgtggtcgat cgctggtgct atctcggcac cgcgagcagt gacccggagg tcgcggagct
     1681 gctcgagacc cgcgagccgc gcttcgacta caaccattac cgcatccttg cgcgccacct
     1741 gggcaggcgc ggcgtgcgtg tggtaccgct cggttactga tgcactgcga actcctggta
     1801 cccggactgt tctcggcggg cgcgcaagcc cgcctgcccg cgctcgaaat gctgctcgcg
     1861 cgcggccgca gccaggccgc gccttcgcag cggctcgagg gctggctgca gcaggcattc
     1921 gacctcgagg ggcagccgct cgccgcgggc gcgctcacgc tgctcggctc cggcggcgat
     1981 cccggagcgg cgcgctgggc gcgcgccgat ccggtccacc tgcgcctgat gcgcgatcgg
     2041 ttgatcctcg tgccggccgc ggcgctcgag atttcgcggc aggaggccga agcgctgtgc
     2101 gcggcgctca gcgcgcattt cgccggcgcg ctggccttcc gtgcggtcga tccgcgccgc
     2161 tggtgcgtgc gcctggagaa ggagctcgcg ctcggcgagg ttgcgccgct cgagatggcg
     2221 ggacgcgagg tactgctcgc gggcgccggc gatgcgctgc tgaacgaggt gcagatgctg
     2281 ctgcatgccc atcccgtgaa cgaggcgcgc gaaacgcgcg gcgagccgcc gctcaacagc
     2341 ctctggctgt ggggcgccgg ccgcgtgccg cgggaggcgc gctgccgctg gcattcgatc
     2401 tcctccgacg atcccctggc gctcggcctg gcgcggctgg ccggcgcgcg tcaccgcggc
     2461 ctgcccgcct cggccggggc ctggctcgat cgtgcacccg aggaagggcg gcacctcgtc
     2521 atcctcgatg cgctgcgcgc gcccctcgcg ctctcacagg aggccgaata ccgcgccggc
     2581 gtcgagacgc tcgagcgcgg ctggttcgct cccctgctcg gcgccttgcg cgaagggcgc
     2641 gtcggcatgg tgacgctgca ggtgcccgac gcggcggccg gcgcttcgtt cgagaccatc
     2701 cgcggcgacc tgcgccgctt ctggcgccgc ccgaaggcgc tcgagcgcta cgcatgaaga
     2761 tcgtcgagcg cccgtaccgc gagtccgatc gccgcgcgct gctcgaggcc ggggtgcacc
     2821 cgatcctcgc ccgcgtctac gccgggcgcc gcatcggttc cgcgcgcgag ctgcgctacg
     2881 acccggccaa cctgctcgcg ccggcgtcgt tgagaaacgc gcagaacgcc gcgcaactgc
     2941 ttgcggcggc gatcgagggc ggcaagcgcc tgctgatcgt ggccgactac gacgctgacg
     3001 gcgccaccgc ctgcgcggtc gggatgcgcg ccctgcgcgc gttcggcgcc acggtcgatt
     3061 acctggtgcc cgaccgcttc aagctcggct atgggctctc gcccgagctg gtggaaatcg
     3121 ccgcggaaag aaagcccgat gtcctgatta ccgtcgacaa cggcatcgcg agcgtcgagg
     3181 gcgtggcgcg cgccagcgag ctcggcatcg ccacgctcat caccgaccat cacctccccg
     3241 gcgccgaatt gcctgcggcc gcgtgcatcg tcaaccccaa ccagccgggt tgcgatttcg
     3301 cctcgaaagc cctcgccggg gtcggcgtca tgttctatgt gatgctcgcg ttgcgcgcgg
     3361 aacttcgaac caggggaaag gagaatgcag agatcaacct cggcgcgctg acggatctgg
     3421 tcgcgctcgg cacgatcgcc gacgtggtgc cactcgacgc gaacaatcgc acgctggtcg
     3481 cgcagggact gaagcggctg cgcgccggta aagcccagcc cggcatcgcc gcactattac
     3541 gtgcggcggg ccgagcacct accgaggcct cgagcttcga cctgggcttc gtggtcgggc
     3601 cgcgcctcaa cgccgccggg cggctagcgg acatgggcct ggggatcgaa tgcctcctca
     3661 cggacgacga ggcgcgcgca gcaaactgcg cccaggcact cgaccgcctg aaccgcgaac
     3721 ggcgcaagat cgaggccggc atgctggaac aggcgatgca ggagctgaaa aagctcgacg
     3781 gcaagagccg gcccgcgttc ttttcgccgc actggcacga aggcgtggtg ggcatcctcg
     3841 cctcgaggct caaggaccgc ctgcaccatc cggtgatctg ctttgcgcgc tcggcacagg
     3901 gcggctggct taaaggctcg ggccgctcca ttcccggact gcatctgcgc gattgcctcg
     3961 atctcgtgtc aaaacgcgcg ccggggctgc tgctgcgctt cggcgggc
//
LOCUS       DASPXW010000246         4946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_578285, whole genome shotgun sequence.
ACCESSION   DASPXW010000246 DASPXW010000000
VERSION     DASPXW010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4946
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578285"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            316..1185
                     /locus_tag="VEL04_05695"
     CDS             316..1185
                     /locus_tag="VEL04_05695"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HYT47893.1"
                     /translation="MSVALEVEVVPTGTFGAEVRGVGLAAVTEAQVDAIKQAWYRHDV
                     LIFRHQEMTDDDLLSFSRYFGTLDPPPNQGAGRKSPPGYPDVYVVSNVLDEDGEPIGA
                     LGDGEAAWHTDMSYAPKPPDASMLYALEIPAKGGDTWFCSMKAALAKMPRRLVERIKH
                     LDIKHDGTYDSGGYLRRGLTASEDPRTSAGTPHPIIIQHPVSGEKALYLGRRRNAYIM
                     GLELAESELLLDKIWSYVDAAVYKHKWALRDVVLWDNRTTMHRRDAFDPGARRVMHRT
                     QIKGSGAPRRAAA"
     gene            complement(1197..2174)
                     /locus_tag="VEL04_05700"
     CDS             complement(1197..2174)
                     /locus_tag="VEL04_05700"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYT47894.1"
                     /translation="MRIFVTKAALDLIAVCVIGAASQGFAQDYPTKPIRFIVPYPPGG
                     GTDVIARIVQPKLSENLGQPIVIENRGGAGGAVGTDAAAKSAPDGYTFLFTLSSHTIN
                     PLLYKLSYDVERDFVPVTMIVSVPQLIAANPQVPVSTLREMVSAAKQRPGAYSFASVG
                     NGTPSHIAGELLKLRAGIDMVHIPYKGGAPAVADTMSGHVPFLIVTAPAALSQVRAGR
                     LRALAVTTLKRTSAAPEIPTVAEALEMPDYEVDSWYALFAPAKTLPAIVSRMQKEVAR
                     VVLLPDVRHKLLEQGADAVGSTPEELDQAVKTELKRWAQVIRDAGIKLE"
     gene            complement(2192..2644)
                     /locus_tag="VEL04_05705"
     CDS             complement(2192..2644)
                     /locus_tag="VEL04_05705"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HYT47895.1"
                     /translation="MSLEQEVELIRQFPIFSKIQPAMQKLLCFSSERLTYETGQVMFN
                     AGDPGDAAYIVIEGSVEISVPTPAGPIIVNTLGRNELIGEIAIFGEVPRTATARALSR
                     LETLKISKELFIKVIRENPDAAIELIKILAGRLANTTSQLTLTRSGGR"
     gene            complement(2688..>4946)
                     /locus_tag="VEL04_05710"
     CDS             complement(2688..>4946)
                     /locus_tag="VEL04_05710"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HYT47896.1"
                     /translation="TMIKDEVEPLGGFIGEALITPLFLGGQAVTAMFFILYQHWVLGM
                     VALGVVGIQAFIIPKLRKRLLVLGRERQLTARALAGRIAECVDGVAEIHAHGTSNYER
                     AEISSRLGTIFRIRFELYQRKFMVKFLNNFLSQVTPFLFYTLGGYLVIVGRLDIGALV
                     AVIAAYKDLPGPVKELIDWDQQRLDVEIKYTQVVEQFTAEDVAPPEQQALIAAPGLPR
                     EGKLRAAGITLIDEAGTRVLEDISFEVGLDEHVAVVGRQGASELAQVVSRLVSPTRGS
                     LELGGVDVTRAPEAVTGRAFSYVGSPAYLFPVSVRDNLLYGLKHAPLREASYEGEALK
                     EREFHLREAERTGNTPLDINADWIDYAAAGCGGAEELEARMVELMRVVELEETIFELG
                     LRSSPDAARAGQIIERVLDARTRMRERLTSLGIQEWVERFDPVRYNRNATLAENLLFG
                     TPVGRTFDIENLAGNEYVRRVLHDTGMHDLLLRAGHKVAETMVELFSGLPPGHEFFAQ
                     YSFIRQEDLPQFEAILKRIIDVGLRGLDETESRLLLSLPFKLVAAKHRLGLIDESFEA
                     RVIEGRRHFAAKLPRELRRAVEFFDPARYNSAASLQDNILFGKIVTGQAEATTRITAL
                     VRELLDELGLRPLVVKIGLDYQVGVGGARLPLADRQKTALVRALLKRPVVLVLDQATA
                     VLDPASQGRVFRGILAERKGRGVLWVLQRPEAAERFSSVLVMERGRLVEQGRFEKLKC
                     SGGTLHKMLAPS"
BASE COUNT          850 a         1629 c         1621 g          846 t
ORIGIN      
        1 ttcccacctg ctccggcttc gccaccggca gcggctgcgc gccgagagcc caggcgcaga
       61 ggacgagcag taaaccggcg aacgtagcgc cggcgcgatt gaatagcgtc atggatccct
      121 ccctggggaa gtccctatcg aacgggcatt aagccaagag cgcgccgcaa catgcaagcg
      181 ccggagtaaa tcagaagcgg atgcgctcga cctgcgtgct cgcgagcttg ccagacaagc
      241 actgtgcatt gaacagcccg gtgacctgcg ctagagtaat cgcagaccgg gacgcaatcc
      301 tcctggagag gcgacatgtc cgttgcattg gaagtcgaag tcgttcccac cggaaccttc
      361 ggcgccgagg tgagaggtgt gggtctcgct gccgttaccg aggcccaggt cgacgccatc
      421 aagcaagcct ggtaccggca cgatgtcctg atatttcggc accaggaaat gaccgacgat
      481 gatcttctgt cgttctcaag gtatttcggc acgctcgatc cgccgccgaa tcagggcgcc
      541 ggccgcaagt cgcctcccgg atatcccgat gtctatgtgg tgtcgaacgt gctcgacgaa
      601 gacggcgagc ccatcggggc gctcggcgac ggcgaagccg cctggcatac ggatatgagc
      661 tatgcgccga aaccgccgga tgcttccatg ctgtatgcgc tcgaaatccc ggccaaagga
      721 ggggacacct ggttttgcag catgaaagcc gcgttggcga aaatgccgcg gcggctcgtc
      781 gagcggatca agcacctgga tatcaagcac gacggcacct acgactcggg cggctacctg
      841 cgcaggggat tgacggcttc ggaggacccg cgcacgtcgg caggcacgcc gcatcccatt
      901 atcattcagc acccggtgtc cggcgagaag gcgctttatc tcggccgcag gcgcaatgcc
      961 tacatcatgg gcctggagct cgcggaatcg gagctgctgc tggacaaaat ctggtcctac
     1021 gtcgatgccg cggtctacaa gcacaagtgg gcgctgcgtg atgtggtgct atgggacaac
     1081 cgcaccacga tgcatcgccg cgacgccttc gatccggggg cgcgccgcgt gatgcatcgc
     1141 acgcagatca aggggtcggg cgcgcccagg cgtgcagcgg cctgacacgg agcgggctat
     1201 tccagtttga tcccggcgtc gcggatgacc tgggcccatc gcttgagctc cgttttcacc
     1261 gcctggtcga gctcttctgg cgtgctgcca acggcatcgg ccccttgctc cagcagttta
     1321 tgcctgacgt cgggaagcaa tacgacgcgg gccacctctt tttgcatccg cgagacgatg
     1381 gccggcagcg tcttcgccgg cgcgaacagc gcgtaccagg aatccacctc gtaatccggc
     1441 atctcgagtg cttcagcgac ggtgggaatc tccggcgcgg ccgaagtgcg cttgagcgta
     1501 gtaaccgcaa gcgcacgcag tcttccggcg cgtacctggg acagggccgc cggggcggtg
     1561 acaatcagga acgggacatg gcccgacatc gtatcggcaa ccgcaggcgc gccaccctta
     1621 tacggaatgt gcaccatgtc gataccggct ctaagcttta acagctcgcc ggcgatgtgg
     1681 gaaggtgttc cgtttcctac ggaggcgaac gaataggctc caggacgttg tttcgccgcg
     1741 ctgaccattt cccgaagcgt gctgaccgga acctgagggt tggccgcgat gagctggggc
     1801 acgctcacga tcatcgttac cggcacgaaa tcccgctcga catcgtaact aagcttgtag
     1861 agcagcggat tgatggtgtg cgaagacagc gtgaacagga acgtatagcc gtccggcgcg
     1921 ctctttgccg cggcgtcagt gccgacggcg ccgccggccc cgccgcggtt ttcgatgacg
     1981 atcggctgcc cgagattttc cgacagcttc ggttgcacga ttcgagcgat gacatcggtg
     2041 ccgccgccgg gggggtacgg cacgatgaag cggattggct tcgtcggata gtcttgcgcg
     2101 aacccttgcg acgcggcgcc gatcacgcag acggcgatca aatcgagcgc tgcctttgtc
     2161 acgaaaattc tcatgccccc tctgagaaaa cctaccgacc gccgctgcgc gtcagcgtga
     2221 gctggctggt ggtattggcg aggcgcccgg cgaggatctt gatcagctcg atcgctgcgt
     2281 ccggattctc gcggatcacc ttgatgaaca gctccttgga gatcttcagc gtctccaggc
     2341 gcgagagcgc cctggcggtg gcggtgcgcg gcacttcgcc aaagatggca atctcaccga
     2401 tcaattcgtt gcgcccgagc gtgttgacga tgatcgggcc ggccggggtc ggcaccgaga
     2461 tctctacgct gccctcgatc acgatgtaag cggcatcgcc cgggtcgccg gcgttgaaca
     2521 tcacctggcc agtctcgtag gtcaggcgct cgctcgaaaa gcacagcagc ttctgcatcg
     2581 ccggctggat cttgctgaag atcggaaact ggcggatcag ctcgacttcc tgttccaggc
     2641 tcatcgtttc tcctgcatct caggccggat tcttggcctt cctttcgtca gctcggcgcg
     2701 agcatcttgt ggagcgtacc gccgctgcac ttcagcttct cgaagcggcc ctgctccacc
     2761 agcctgccgc gctccatcac cagcaccgag ctgaagcgct cggcggcctc cgggcgctgc
     2821 agcacccaga gcacgccgcg gcccttgcgc tcggcaagaa tgcccctgaa cacccggccc
     2881 tgcgaggccg ggtcgagcac cgcggtcgcc tggtcgagga ccagcaccac ggggcgcttg
     2941 agcagtgcgc gcaccagggc agtcttctgc cggtcggcca gcggcagccg ggcgccgccc
     3001 acgccgacct ggtaatcgag cccgatcttc accaccagcg ggcgcaggcc gagctcatcg
     3061 agcagctcgc gcaccagcgc ggtgatgcgc gtcgtggcct cggcctggcc ggtgacgatc
     3121 ttgccgaaga ggatgttgtc ctgcagcgat gccgcgctgt tgtagcgcgc cgggtcgaag
     3181 aactcgaccg cgcgccgcag ctcccgcggc agcttggcgg cgaagtgcct ccgcccttcg
     3241 atgactcgcg cctcgaagct ctcgtcgatc aatcccaggc ggtgcttcgc ggcgaccagc
     3301 ttgaagggca gcgagagcag cagccgactt tccgtctcgt cgaggccgcg caggccgacg
     3361 tcgatgatgc gcttcaggat cgcctcgaac tgcggcaggt cctcctgccg gatgaagctg
     3421 tactgcgcga agaattcatg gcccggcggc agcccgctga acagctcgac catggtctcg
     3481 gcgaccttgt ggccggcgcg cagcagcaga tcgtgcatcc cggtgtcgtg cagcacgcgg
     3541 cgcacgtact cgttgccggc gaggttctcg atgtcgaacg tcctgccgac cggcgtaccg
     3601 aagagcaggt tctcggcgag cgtcgcgttc ctgttgtagc gcaccgggtc gaagcgctcg
     3661 acccactcct ggatgccgag ggaggtcagg cgctcgcgca tgcgcgtacg cgcgtcgagc
     3721 acgcgctcga tgatctggcc ggcgcgcgcc gcgtcgggcg agctgcgcag cccgagctcg
     3781 aagatggtct cttccagctc gaccacgcgc atcagctcga ccatgcgcgc ctcgagctcc
     3841 tcggcgcccc cgcagcctgc ggcggcgtag tcgatccagt cggcgttgat gtcgagcggc
     3901 gtattgccgg tgcgctcggc ctcgcgcaga tggaactcgc gctccttcag cgcctcgccc
     3961 tcgtagctcg cctcgcgcag cggcgcgtgc ttgaggccgt agagcaggtt gtcgcgcacg
     4021 ctcaccggaa agaggtacgc gggcgagccg acataggaga acgcgcgccc ggtgaccgcc
     4081 tccggcgcgc gcgtgacgtc gacgccgccg agctcgagcg agccgcgcgt aggcgacacg
     4141 aggcgcgaca cgacctgcgc gagctcgctc gcgccctggc ggccgaccac cgcgacgtgc
     4201 tcgtcgagcc cgacctcgaa gctgatgtcc tcgaggacgc gcgtcccggc ctcgtcgatg
     4261 agcgtgatgc cggcggcgcg cagcttgcct tcgcgcggca gcccgggcgc ggcgatgagc
     4321 gcctgctgct ccggcggcgc cacgtcctcg gcggtgaact gctcgaccac ctgggtgtac
     4381 ttgatctcga cgtcgaggcg ctgctggtcc cagtcgatca gttccttgac cggtcccggg
     4441 aggtctttgt aggcggcgat taccgcgacc agcgcgccga tatcgagcct gccgacgatc
     4501 accaggtagc cgcccagcgt gtagaacagg aacggcgtca cctgcgacag gaaattgttg
     4561 aggaacttga ccatgaactt gcgctggtag agctcgaagc ggatgcggaa gatcgtgccc
     4621 aggcgcgagg agatctcggc gcgctcgtag ttcgaagtgc cgtgcgcgtg aatctcggcg
     4681 acgccgtcga cgcactcggc gatgcgtccg gcgagcgcgc gcgccgtgag ctgccgctcg
     4741 cgcccgagca ccagcaggcg cttcctcagc ttcgggatga tgaaggcctg gatgccgacc
     4801 acgccgagcg ccaccatgcc gagcacccag tgctggtaga ggatgaagaa catcgccgtg
     4861 accgcctgcc cgccgaggaa cagcggcgtg atcagcgcct cgccgatgaa gccgccgagc
     4921 ggctcgacct cgtccttgat catggt
//
LOCUS       DASPXW010000247         2108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_75911, whole genome shotgun sequence.
ACCESSION   DASPXW010000247 DASPXW010000000
VERSION     DASPXW010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75911"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VEL04_05715"
     CDS             complement(<1..440)
                     /locus_tag="VEL04_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HYT47897.1"
                     /translation="MIDYNVFRQFGEAIRHLGEMAQKVELADDERVERAKTVMLTKID
                     RQMAVDLESCVKCGYCAEACHFYVETGDPKYVPTRKLDLLRRVYGREVSPLRFVRRLF
                     EREITADELREWQELVYDSCTECGRCSYACPMGINIARGVNVMR"
     gene            complement(437..1114)
                     /locus_tag="VEL04_05720"
     CDS             complement(437..1114)
                     /locus_tag="VEL04_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47898.1"
                     /translation="MDLLELARGPLWYAAGAIFILGTAWRLYGIWRLGTRPVLAAPRS
                     AATTSAAIRTVFSRMVPRAGFHASATLATVNPYLYHVGLAIVAFGYLPHIEFVRRVTG
                     VSWPALPDWVMLVAAGLTIVSLGIALLYRLTDPVLRLISGIGDYFGWIVTLLPLVTGM
                     ALISEPSAALHARQAISNPLPLAIHLLSLELLLAWFPFGKLMHAFLVVPARAQLGAFF
                     ARRGVQA"
     gene            1215..1409
                     /locus_tag="VEL04_05725"
     CDS             1215..1409
                     /locus_tag="VEL04_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HYT47899.1"
                     /translation="MTSNVGTADRIIRAVLGLALLSLILVLEGNARWWGLVGLVPLAT
                     TFIRWCPAYVPFGISTTRTG"
     gene            1411..2028
                     /locus_tag="VEL04_05730"
     CDS             1411..2028
                     /locus_tag="VEL04_05730"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYT47900.1"
                     /translation="MSELARWNQRFSAPDYIFGTAPNEFLAAQAGRLRPGQRALCIAD
                     GEGRNSVWLAERGLEVVAFDVSPVGVEKARRLAAQRGVTAAFEVADVYGWSWPAAAFD
                     AIAAIFVQFADPPMRGFMFERIARALKPGGLLIMQGYTPRQVEYKTGGPPRAENMYTP
                     ELLRAAFASLEILELREHERELAEGSQHLGRSALIDLVARKSAVP"
BASE COUNT          370 a          682 c          700 g          356 t
ORIGIN      
        1 tcgcgcatca cgttcactcc gcgcgcgatg ttgattccca tcgggcacgc atagctgcag
       61 cgcccgcact cggtgcagga gtcgtacacc agttcctgcc attcgcgcag ctcgtcggcg
      121 gtgatctcgc gctcgaatag gcgccgcacg aaacgtagcg gcgaaacctc gcggccgtac
      181 acgcgccgca aaagatccag cttgcgcgtc gggacgtact tcggatcgcc cgtctcgacg
      241 tagaaatggc aggcctcggc gcagtagccg catttcacgc acgactcgag atcgacggcc
      301 atctgccgat cgatcttggt cagcatgacg gttttggctc gctcgacgcg ttcgtcatcc
      361 gccagctcta ccttttgcgc catttccccg agatgcctga tggcttcgcc gaactgccgg
      421 aagacgttgt aatcgatcat gcctgcaccc cgcgacgggc gaagaaagcg ccgagctggg
      481 cgcgcgccgg caccaccaga aaagcgtgca tcagcttccc gaacgggaac catgcgagca
      541 gcagctcgag cgagagcagg tggatcgcga gcggcaatgg attggagata gcttgccttg
      601 cgtgcaatgc ggccgagggt tcgctgatca acgccatgcc ggtgacgagc ggcagcaacg
      661 tgacgatcca gccgaaatag tcgccgatgc ctgagatcag ccgcagcacc ggatcggtga
      721 gccggtaaag cagcgcgatg ccgagcgaga cgatcgtcag gccggcggcg acgagcatca
      781 cccaatccgg aagcgcgggc caggaaacac ccgtgacccg cctgacgaac tcaatgtgcg
      841 gcagatagcc gaacgcgacg atcgccagtc ccacgtgata gagatacggg ttgaccgtgg
      901 cgagcgtggc gctcgcatgg aagccggctc tcggcaccat gcgtgaaaac accgtccgta
      961 tagcggcgga ggtggtagcc gcgctgcgcg gggcggcgag cacaggtcta gtgccgagcc
     1021 gccagatccc gtacagccgc cacgccgtgc cgaggatgaa aattgccccc gccgcgtacc
     1081 acagcggtcc tcttgcgagc tcgagtaggt ccatggcctc acgttacgag cagaggagcc
     1141 cgcggatttg atccatgtca aacggcggcg ggggaccggc agtatgtttg cgggtaccct
     1201 tacaggagaa acgcatgaca tcgaacgtcg gaactgctga tcgcattatt cgggcggtgc
     1261 tcggcctggc tcttctgagc ctcattttgg tcctggaggg caatgcccgc tggtgggggc
     1321 tggtcggcct cgtgccgctc gctaccactt tcatcaggtg gtgcccggcc tacgtaccgt
     1381 ttggcatcag caccacgcgg accggctgag ttgagcgaac tcgcgcgctg gaatcagcgc
     1441 ttctcggcac cggattacat cttcggcacc gcgccgaacg agttcctcgc cgcgcaggcc
     1501 gggcggttgc ggccggggca gcgcgctctg tgcatcgcgg atggcgaggg acgtaacagc
     1561 gtctggctcg ccgagcgcgg gctcgaggtc gtggccttcg acgtctcgcc ggtcggcgtc
     1621 gagaaggcga ggcgcctggc ggcgcagcgc ggcgtcacgg ccgccttcga ggtcgcggac
     1681 gtctacggct ggagctggcc ggcggccgcc ttcgacgcga ttgcggcgat cttcgtgcag
     1741 ttcgccgatc cgccgatgcg cggattcatg ttcgagcgca tcgcgcgcgc gctgaagccg
     1801 ggcggcctcc tcatcatgca gggctacacg ccgaggcagg tcgagtacaa gaccggcggt
     1861 ccgccccgcg ccgaaaacat gtatacgccc gagctgctgc gcgccgcatt cgcttcgctc
     1921 gagatcctgg agctgcgcga gcacgagcgc gagctggcgg agggcagcca gcaccttggc
     1981 cgctcggcac tgatcgatct cgtggcgcga aaatccgccg tgccctgatg gagcgatgcg
     2041 caccctgcgt ccaggccctg atctgcggca tttcagccgc gttgtttgcc gttttctccg
     2101 cggcgcag
//
LOCUS       DASPXW010000248         7948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_199588, whole genome shotgun sequence.
ACCESSION   DASPXW010000248 DASPXW010000000
VERSION     DASPXW010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7948
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199588"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /gene="ribD"
                     /locus_tag="VEL04_05735"
     CDS             complement(<1..1010)
                     /gene="ribD"
                     /locus_tag="VEL04_05735"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006756145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HYT47901.1"
                     /translation="MMRRALELAAKGLYTTSPNPRVGCVVAKEEAIAGEGWHEKAGGP
                     HAEVVAIGNAGNRAEGATLYVNLEPCSHQGRTPPCVDLIRKFKLRRVVAAMLDPNPKA
                     AHGGEALAAAGIRFEHGLMEDEANELNIGFVSRMARGRPWVRLKVAATLDGRTALADG
                     RSQWITGPEARRDGHRWRARACAILTGIGTVKADDPRLTVREVETPRQPLRVIVDSRL
                     ETPATARILEGGNVLIFSGKSGAGPKGAEVICLPNKNLKVELPQMLEELARRGVNELH
                     VEAGFRLNGSLTREGCVDEFLLYLNPSLLGDGAQGMLDLPALASLEKRLKLRTVSLDR
                     LG"
     gene            complement(1037..1504)
                     /gene="nrdR"
                     /locus_tag="VEL04_05740"
     CDS             complement(1037..1504)
                     /gene="nrdR"
                     /locus_tag="VEL04_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HYT47902.1"
                     /translation="MKCPFCGSEDTQVVDTRANLEANTTRRRRKCVACEKRFTTYERV
                     DLKMPRLVKKDGSRTDFDRDKLMGSMMLALRKRPVATDEVEGAVDRVIERLRSLGERE
                     VPSSRVGDFVMRELAKLDKIGYIRFASVYRSFETPDDFREAVQEVKSTRKRRR"
     gene            complement(1501..2742)
                     /gene="glyA"
                     /locus_tag="VEL04_05745"
     CDS             complement(1501..2742)
                     /gene="glyA"
                     /locus_tag="VEL04_05745"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HYT47903.1"
                     /translation="MFAPNLLAQADPEVWNAVRLEHQRQEDHVELIASENYVSRPVLI
                     AQGSQLTNKYAEGYPGRRYYGGCEYVDMAETIAIERAKQLFGAPWANVQPHSGSQANQ
                     AVYTAVLKPGDTILGMSLAHGGHLTHGSPVNLSGKLYRVVSYGLTEKEVIDYDAVERI
                     ALAERPKLLVAGASAYALAIDWKRFRHIADQVGALLMADIAHYAGLIATGLYPSPVGI
                     ADFVTTTTHKTLRGPRGGMILGKAEQERAINSSIFPGLQGGPLMHVIAAKAVSLGEAL
                     KPDFKVYQSRVLENARTMAKVLTERGVRVVSGRTDCHLFLLDLRKQGISGKDAESALG
                     RAHVTVNKNAIPNDPEKPTVTSGVRIGTPAMTTRGFRRAEAEVLAHLVADVLERPHEN
                     ASLERVAGEVKALCARFPVYA"
     gene            complement(2748..3695)
                     /locus_tag="VEL04_05750"
     CDS             complement(2748..3695)
                     /locus_tag="VEL04_05750"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYT47904.1"
                     /translation="MASAAIIINGEETGIAETSTRRAILFADVCDSTAIYEAIGDAKA
                     HALINRLLALLGRKVKAAGGAVVKTLGDGIVCQFGSADAAYRAACEMQVAAHRVTAPG
                     ARKLAIKVGYTYGPVVPKVSDVFGDTVNLCARLVALANPGQVLTTQQTVEAQSPGLRQ
                     RCRALYETKVRGRASQVTVCEVLWRTDPDVTELKLTQEGLARASQWVLKLTYGDDSYT
                     VESTAAVKIGRDKSNDVVVPSQHASRLHARLFGRDGNFVIVDQSSNGTFVMIDGNTRE
                     LRLRREEAVIGEGGKIGLGTSASTDGDHVLRYRVERRGS"
     gene            3694..4071
                     /locus_tag="VEL04_05755"
     CDS             3694..4071
                     /locus_tag="VEL04_05755"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYT47905.1"
                     /translation="MRAARRILIALLAIVACAAAAQAPWPERPVKQQFVVENMPGGSG
                     NIGAAVAARAAPDGYTFLVSPEPALTVNLSLFRNLPYNAERDFVPVACGVTVPLVLCA
                     RASLDVRTLAGLVEIGRRAPSRP"
     gene            complement(4061..5038)
                     /locus_tag="VEL04_05760"
     CDS             complement(4061..5038)
                     /locus_tag="VEL04_05760"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HYT47906.1"
                     /translation="MEPLPKGASFIRAKFLWEIGLHIAGNPATPYYGNRDLCIAIGSG
                     SAASYRPWLRMSTGSPILAHAVARNELDLAIVNPSAMLTQAYRGKGLFREPLPLRIVA
                     NYPSWDRFAYVLHPRTAISSLAEIKARRYPLRLSTREDPTHSTRNLIDQTLAIHGFSI
                     SELESWGGSLQPNGGPGDQRRIDALAAGTIDAIFDEGLALWFDAALAAGMRPITLEAE
                     TFRQLAELGWRKVVIPAGRYRHLESDYACIDYSGWPLYSRVDLPEDDAYKVCDAIHAR
                     EAEIVWEDTNGWAPHAGMGPLGQETEATPRDVPLHPGAARWFREHGFKV"
     gene            complement(5048..5821)
                     /locus_tag="VEL04_05765"
     CDS             complement(5048..5821)
                     /locus_tag="VEL04_05765"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYT47907.1"
                     /translation="MDLQLKDKIAIVGGGSDGIGHGIAMALAAEGAKVAIYARREAGV
                     SRAAQAIREATSAEVLGLQADAKSADDCARVIEKAAAHFGGLDIVVNNDGAPPLGAIS
                     SFDDAAWQKAVEQNLMYAIRMSRGALPQLQRRGGGSILNITAISAIQPIAGFGLSVAT
                     WAGVIGFAKTLSLEAAPMNISVNTICPGYIETKRLEKVFTGDDPQAMRRKLVSEIPLG
                     RIGTVADVANLVAYLVSPLGRYVTGTTIQVDGGLLRALR"
     gene            complement(5821..6678)
                     /locus_tag="VEL04_05770"
     CDS             complement(5821..6678)
                     /locus_tag="VEL04_05770"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYT47908.1"
                     /translation="MKLIDVHSHWGTKRGYPLQTGEELALQRQTWNSEVRYHTEAEMA
                     QHFRDHGVQAILDLGFAKYRPLEEMQALHDYAFEVESQHPEVILGHWVHVDPLRTLDG
                     VRELRRCIDKRRGFFGFAVSSSLTPPASDPSFDPYYALCREAGIPVLIFVGTTGLGAG
                     LPAGGGVMLEHCHPRHLDNVAARYPGLHIVAGRPGWPWQTETIAVLLHKRHVWYELHG
                     WSPKYHTPELKHEIARRLRNRILFAADYPLFSYERLVRDWCAEGYAQEVLEQVFHGNA
                     ENFLKNLPG"
     gene            6822..7565
                     /locus_tag="VEL04_05775"
     CDS             6822..7565
                     /locus_tag="VEL04_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010926057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase/reductase"
                     /protein_id="HYT47909.1"
                     /translation="MDLGLSGKSVLVTGGSKGIGLACAKAFAAEGCHVHLAARDRARL
                     EKTKSELGGRATVHGVDLRDGKALCGLAEACRDVDILVNNAGDIPGGTLASVDEEKWR
                     HAWELKVFAYINLSRSMYARMQARKSGVIVNVIGMAAEKPAFEYICGSTANAGLAAFT
                     KALGKGMKPHGVRVLGVHPPSTRTDRIVTVMKGAAKAKFGDESRYQELAGEGPFAGLI
                     EPEQVADVVAFLASPRCAHLTGVVLNLGP"
     gene            complement(7570..>7948)
                     /locus_tag="VEL04_05780"
     CDS             complement(7570..>7948)
                     /locus_tag="VEL04_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYT47910.1"
                     /translation="HDDMERMFVRFPILKERITQSAGTLSGGEQQMLAVARALVTDPR
                     IILLDEPSQGLAPLVVEELIRLIRRLRTEGVSFLLVEQNLKLAEALADVVYVMLKGQT
                     VYRAPLAQFAAEREDVKARYLML"
BASE COUNT         1230 a         2643 c         2695 g         1380 t
ORIGIN      
        1 tcgccgaggc ggtcgagcga cacggttctt aatttgagcc gcttttccag ggaagcgagc
       61 gccggcaggt cgagcatgcc ctgcgcacca tcgccgagca agctcgggtt gaggtagagc
      121 aggaactcgt cgacgcagcc ttcgcgcgtc agcgagccat tgaggcgaaa gcccgcctcg
      181 acgtgcaact cgttgacgcc gcgccgcgcc aattcctcga gcatttgggg caactccacc
      241 ttcaggttct tgttcggcaa gcaaatgact tcggcgccct tggggccggc gccgcttttc
      301 cccgagaaaa tcaagacatt tcccccctcg agaatgcggg ctgtcgcggg cgtctcgagc
      361 cggctgtcga cgatcacgcg cagcggctgg cgcggcgtct cgacttcgcg caccgtgagg
      421 cgcggatcgt cggccttgac cgtgccgatg ccggtgagga tcgcacaggc gcgcgcgcgc
      481 cagcggtggc cgtcgcgccg cgcttcgggc ccggtgatcc attgcgagcg cccgtcggca
      541 agagcagtgc gcccgtccaa cgtcgccgcg accttgaggc gcacccaggg ccggccgcgt
      601 gccatgcgcg agacgaagcc gatgttgagc tcgttagcct cgtcctccat cagcccgtgc
      661 tcgaaacgga ttcccgcggc cgccagcgcc tcgccgccgt gcgcggcctt cgggttcgga
      721 tccagcatcg ctgccaccac acggcgcagc ttgaattttc ttatgagatc cacgcaaggc
      781 ggggtgcggc cctggtggct gcagggctcg agattgacgt acagcgtcgc gccctcggcc
      841 cggtttcccg cgtttccgat ggcaaccact tcggcgtgcg gcccgcccgc cttctcgtgc
      901 catccctcgc ccgcgattgc ttcttccttt gcaaccacac aacccacgcg cggattcggc
      961 gatgtcgtgt agaggccttt cgcggcgagc tcgagcgcac gccgcatcat ggcgtggtca
     1021 aaagcgctga accgcgtcac ctgcgtcttt tgcgcgtcga cttgacttcc tgcaccgcct
     1081 cgcggaaatc gtcgggcgtc tcgaagctgc ggtaaaccga ggcgaagcgg atgtaaccga
     1141 tcttgtcgag cttggcgagc tcgcgcatca cgaagtcgcc gacccggctg ctcggcacct
     1201 cgcgctcgcc gagcgagcgc aggcgctcga tcacgcgatc gaccgcgccc tcgacctcgt
     1261 cggtcgccac cgggcgcttc ctcagcgcga gcatcatgct gcccatcagc ttgtcgcggt
     1321 cgaagtcggt gcggctgccg tctttcttca ccagccgggg catcttcagg tcgacgcgct
     1381 cgtaggtggt gaagcgcttt tcgcaggcga cgcacttcct gcggcggcgg gtcgtgttcg
     1441 cctcgaggtt ggcgcgcgta tcgaccacct gcgtgtcctc ggagccgcag aaagggcact
     1501 tcatgcgtac accgggaacc tggcgcagag cgccttcact tcacccgcca ccctctcgag
     1561 gctcgcgttc tcgtgcggcc gctcgagcac gtcggcgacc agatgcgcca ggacctcggc
     1621 ctcggcgcgc cggaagccgc gcgtggtcat ggcgggggtg ccgatgcgta cgccgctcgt
     1681 gacggtgggc ttttccggat cgttcgggat cgcgttcttg ttcacggtga cgtgcgcgcg
     1741 cccgagcgcg gactcggcat ccttgccgct gatgccctgc ttgcgcagat cgagcaggaa
     1801 caagtggcag tcggtgcgcc ccgacacgac ccgcacgccg cgctcggtga gcactttcgc
     1861 catcgtgcgc gcgttctcca gcacccggga ctggtaaacc ttgaagtcgg gtttcagggc
     1921 ttcgcccagc gacacggcct tggcggcgat cacatgcatc agagggccgc cctgcaaacc
     1981 gggaaagatc gagctgttga tggctctttc ttgctcggcc ttgcccagga tcatgccgcc
     2041 gcgcggcccg cgtaaggtct tgtgcgtggt ggtggtgacg aagtccgcga tgccgaccgg
     2101 cgagggatag agcccggtgg cgatgaggcc ggcgtaatgc gcgatatcgg ccatcagcag
     2161 cgcgccgacc tggtcggcga tgtggcggaa gcgcttccag tcgatcgcga gagcgtacgc
     2221 cgaggccccg gcgacgagga gcttcggcct ttccgcgagc gcgatccgct cgacggcgtc
     2281 gtagtcgatc acctcctttt cagtcagccc ataggagacg acgcggtaga gcttgcctga
     2341 aaggttgacc ggcgagccgt gcgtgagatg gccgccgtgc gcgagcgaca tccccaggat
     2401 ggtgtcgccg ggcttgagca ccgcggtgta gaccgcctgg ttcgcctgcg agcccgaatg
     2461 cggctgcaca ttggcccacg gcgcgccgaa cagctgcttc gcacgctcga tcgcgatggt
     2521 ctcggccatg tcgacgtact cgcagccgcc gtagtagcgc cgcccaggat agccttcggc
     2581 atatttgttg gtgagctggc tgccctgcgc gatcagcacc ggacggctga cgtaattttc
     2641 cgaggcgatc agctcgacgt gatcttcctg ccgctggtgc tcgaggcgca ccgcgttcca
     2701 gacttcggga tcggcctggg cgaggagatt gggagcaaac atggctgtca gctgccgcgg
     2761 cgctcgacgc ggtagcgcag cacgtggtcg ccgtcggtcg acgccgaggt gccgaggccg
     2821 atcttgccgc cctcgccgat cactgcctcc tcgcgcctca gcctcagctc gcgcgtgttg
     2881 ccgtcgatca tcacgaaggt gccgttggag gactggtcga cgatgacgaa attgccgtcg
     2941 cgcccgaaca agcgcgcatg cagccgcgag gcgtgctggc tcggcaccac cacgtcgttc
     3001 gacttgtcgc gcccgatctt caccgcggcg gtggactcga ccgtgtagct gtcgtcgccg
     3061 taggtaagct tgagcaccca ttggctggcg cgcgccaggc cttcctgggt cagcttcaat
     3121 tcggtgacgt cggggtcggt gcgccacagc acctcgcaca ccgtcacctg gctggcgcgt
     3181 ccacgcacct tggtctcgta gagcgcccgg cagcgctggc gcagccccgg cgactgcgcc
     3241 tcgacggtct gctgggtagt gagcacctgc ccggggttcg caagcgcaac cagccgcgcg
     3301 cacaggttca cggtgtcacc gaagacatca ctgactttcg gtaccaccgg gccgtaggta
     3361 tagccaacct ttatggcgag cttgcgcgcg ccgggcgcgg tcacgcggtg ggcggccacc
     3421 tgcatctcgc aagcggcgcg atacgcggcg tcggcgctgc cgaactggca cactatgccg
     3481 tcgccgaggg tcttgaccac cgcgccgccc gcggccttga ccttcctgcc gaggagcgcc
     3541 agcaggcggt tgatgagcgc gtgcgccttc gcatcgccga tcgcttcgta gatcgcggtg
     3601 ctgtcgcaaa catcggcaaa caggattgcg cgccttgtgc tggtttctgc gatgccggtt
     3661 tcctccccgt tgattatgat agcagcggat gccatgagag ccgcccgccg cattttgatt
     3721 gcgctacttg ctattgtggc ctgcgcggcg gcggcgcagg cgccctggcc agagcggccg
     3781 gtgaagcagc agttcgtagt ggagaacatg ccgggcggca gcggtaatat cggcgccgcg
     3841 gtagccgcga gagcggcgcc ggacggctac accttcctcg tctcgccgga gccggcgctg
     3901 acggtgaatc tgagcctgtt caggaacctg ccgtacaacg cggagcgcga cttcgttccc
     3961 gtcgcctgcg gcgtgacggt gccgctcgtt ctctgcgccc gcgcctcgct cgatgtgcgc
     4021 acgctggccg gcctggtgga aatcgggcgg cgcgcccctt ctagaccttg aagccgtgct
     4081 cgcggaacca gcgcgccgcg ccgggatgca gcggcacgtc gcgcggtgtc gcctcagttt
     4141 cctgcccgag cggtcccatg ccggcgtgcg gcgcccagcc gttcgtgtcc tcccagacga
     4201 tctcggcttc gcgcgcatgg atggcgtcgc agaccttgta agcgtcgtcc tcggggaggt
     4261 cgacgcgcga atacagcggc cagccgctgt agtcgatgca cgcgtaatcg gactcgaggt
     4321 ggcggtagcg cccggctgga atcaccacct tgcgccagcc gagctccgcc agctggcgaa
     4381 aagtttccgc ttcgagagtg atcggccgca tcccggcggc gagcgcggca tcgaaccaca
     4441 gcgcgagccc ttcgtcgaag atcgcgtcga tcgttccggc ggcgagcgcg tcgatgcgcc
     4501 gctggtcccc cggcccgccg ttcggctgca ggctgccgcc ccagctttcc aattcggaaa
     4561 ttgaaaaacc atggatcgcg agcgtctgat cgatgaggtt gcgcgtcgag tgcgtcgggt
     4621 cctcgcgggt agagagccgc agcgggtagc gccgcgcctt gatctcggcc agcgagctga
     4681 ttgcggtgcg cgggtgcagc acgtaggcaa accggtccca cgacggatag ttggcgacga
     4741 tgcgcagcgg cagcggctcg cggaacagcc ccttgccgcg gtacgcctgg gtcagcatcg
     4801 ccgagggatt gacgatcgcg aggtcgagct cgttgcgcgc caccgcgtgg gcgaggatcg
     4861 gagacccggt cgacatgcgc aaccacggac ggtacgaggc ggccgagccg ctgccgattg
     4921 cgatgcagag gtcgcggttg ccgtagtacg gcgtcgccgg attgccggcg atgtgcaggc
     4981 cgatttccca gagaaacttg gcgcggatga agctcgcgcc cttgggcagc ggttccatgc
     5041 tttctcctca gcgcagcgcg cgcagcagcc cgccatcgac ctggattgtc gtaccggtga
     5101 cgtagcgccc caggggcgag acgaggtagg caacgaggtt ggcgacgtcg gccaccgtgc
     5161 cgatgcgccc cagcgggatc tccgagacga gcttgcggcg catggcctgc gggtcatcgc
     5221 cggtgaacac cttttccagg cgcttggtct cgatgtagcc ggggcagatc gtattcacgc
     5281 tgatgttcat gggcgcggct tcgagggaga gcgtcttggc gaaaccgatc acgccggccc
     5341 aggtcgcgac cgaaaggccg aatccggcga tcggctggat cgccgagatc gcggtgatgt
     5401 tgaggatgct gccgccgccg cggcgctgca gctgcggcag cgcgccgcgc gacatgcgga
     5461 tggcgtacat caggttctgt tcgaccgcct tctgccacgc ggcatcgtcg aaagagctaa
     5521 tggccccgag cggcggcgcg ccgtcgttgt tcaccacgat gtcgaggccg ccgaaatgcg
     5581 ccgccgcctt ctcgatcacc cgggcgcagt cgtctgcgct ctttgcgtca gcctgaagcc
     5641 caagaacttc ggcgctggtg gcttcgcgaa tcgcctgggc tgcgcgcgag acgcccgcct
     5701 cgcggcgcgc gtaaatcgcg accttcgcgc cctcggcggc gagcgccatc gcgatgccgt
     5761 gtccgatgcc gtcgctcccg ccgccgacga tggcgatttt gtcttttaat tgaagatcca
     5821 ttatcccggg agattcttaa ggaagttctc cgcgttcccg tggaaaacct gctcgagcac
     5881 ctcctgcgcg tagccttcgg cgcaccagtc gcgcaccagg cgctcgtagg agaaaagcgg
     5941 atagtccgcg gcgaacagga tccggttcct gagccggcgc gcgatctcgt gcttcagctc
     6001 gggcgtgtgg tacttgggcg accagccgtg cagctcgtac cagacgtggc gcttgtgcag
     6061 gagcaccgcg atggtctcgg tctgccacgg ccagccggga cgcccggcga cgatgtgcag
     6121 gcccgggtag cgcgccgcca cgttgtcgag gtgccgggga tggcagtgct cgagcatcac
     6181 gcccccgccc gcgggcaggc cggcgccgag cccggtggtt ccgacgaaga tgaggaccgg
     6241 gatgcccgcc tcgcggcaca acgcgtagta gggatcgaag gaaggatcgc tcgccggcgg
     6301 ggtgagcgag ctcgacaccg cgaagccgaa gaagccgcgc cgcttgtcga tgcagcggcg
     6361 cagctcgcgc acgccgtcca gcgtcctgag aggatcgacg tggacccagt ggccgaggat
     6421 gacctcggga tgctggcttt ccacctcgaa ggcatagtcg tgcagcgcct gcatctcctc
     6481 cagcggccgg tatttggcga atccgaggtc gagaatcgcc tgcacgccgt gatcgcggaa
     6541 gtgctgcgcc atttcggcct cggtgtgata gcgcacctcc gaattccacg tctggcgctg
     6601 cagcgcgagc tcctccccgg tctgcagcgg atagccgcgc ttcgtgcccc agtgcgaatg
     6661 cacgtcgatg agcttcatgg cagcgattat tttgcctgtc ctatactcgc cgctccatcc
     6721 ttctgcgaaa tgcgcgcgcc gagctgaaaa actgacccgg cgcgctttcc ggcggccacg
     6781 ctcccatagt aggctggctg cgtgaagcca cccgggggcc catggatctc ggtctaagcg
     6841 gcaagtccgt gctggtcacg ggcggttcga aaggcatcgg cctcgcgtgc gccaaggcgt
     6901 tcgccgcgga aggctgccat gtccatctcg ccgcgcgcga tcgcgcgcgg ctggagaaaa
     6961 cgaagtctga actcggtgga cgcgcgacgg tgcatggggt ggacttgcgc gatggcaagg
     7021 ccctgtgcgg gctggcggag gcgtgcaggg atgtggacat cctggtcaac aacgcaggag
     7081 acattccggg cgggacgctg gcgtcggtcg acgaggagaa atggcggcac gcgtgggagc
     7141 tcaaggtgtt cgcctacatc aatctcagcc gctcgatgta tgcgcgcatg caagcgcgga
     7201 aaagcggcgt catcgtcaac gtcatcggca tggcggccga gaaaccggcg ttcgagtaca
     7261 tctgcggcag cacggcgaac gcgggccttg ccgccttcac caaggccctg ggcaagggaa
     7321 tgaagccaca cggcgttcgg gtgctcggcg ttcacccgcc ctcgacgcgc accgaccgga
     7381 tcgttacggt gatgaaaggc gcggcgaagg cgaaattcgg cgacgagagc cgctaccagg
     7441 agctcgcggg agaagggccg ttcgccgggc tcatcgagcc cgagcaggtc gccgacgtcg
     7501 tcgcctttct tgcctcgccc cgctgcgccc acctcacggg tgtggtcctc aacctgggcc
     7561 cgtagggctc tagagcatca gatagcgggc cttcacgtcc tcgcgctcgg cagcgaactg
     7621 cgcgagcggc gcgcggtaga cggtctggcc tttcagcatc acgtagacga cgtcggccaa
     7681 ggcttcggcg agcttcaggt tctgctcgac gaggaggaag ctcacgcctt ccgtgcgcag
     7741 ccgccggatc agccggatga gctcctccac cacgaggggc gccagaccct gcgacggctc
     7801 gtccagcagg atgatgcgcg gatcggtgac gagcgcgcgc gccaccgcca gcatctgctg
     7861 ctcgccgccc gagagggtgc cggccgactg cgtgatgcgc tccttcaaga tcgggaagcg
     7921 cacgaacatg cgctccatgt cgtcgtgg
//
LOCUS       DASPXW010000249         2590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_954807, whole genome shotgun sequence.
ACCESSION   DASPXW010000249 DASPXW010000000
VERSION     DASPXW010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954807"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2590
                     /locus_tag="VEL04_05785"
     CDS             <1..>2590
                     /locus_tag="VEL04_05785"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HYT47911.1"
                     /translation="WSGEDDIFLDFDPERGIVAGQRWARALEDAATRCEAVLFLVSEV
                     WLASKWCGDEYQLANRLNKKLFALLIDDISLDRLPGGLTAQWQVVPLKGEPAERFVTV
                     HPRTQHQSPVHIAEAALKSLKRGLEKAGIGPETFELQPDATGPFGWRAPYRGLEALEP
                     EDAAVFFGRSADIVRGIDALRGLAARKSPRLLVVLGASGAGKSSYLGAGLWPRLLRDD
                     SQWLPLRAIRAGRGGAIEGSEGLLHALEDVHRRFALRASRSDLRQRLATPESFVALLQ
                     ELRGAAARRALISAPPQPLPVLCLDQGEELFAADAGAESESFLRLARAAVDADAALLL
                     VTIRSDAYGLMQSAGTLTGIDQVPLSLGPVPQGEIARIIREPSEVLRRKAGPSAPIFD
                     AALVERLQSEIAGEMDALPLLAFVLQRLMREHAGTSAIGVRELDQTGDVAAAIEQEAE
                     AALADAVFGPDRAERREVLRRLFVPRLARIDRASKAPQRRVARQSDFPVDLLALARAL
                     TQRRLLVTKLASADTTESDAGTFEAGAATLEVAHEALLRRWPTLADLLREDRDALILL
                     DGVLIAATDWDKADAARRSDFLAHRGSRLVDAQALASRGPEWAREIAPAGPYLAACAL
                     REIEERKEKEDALAREQNRLAEIATAQERTGRLQRLTRWAFAAVGAVVVIAVVIVGWL
                     QWDKGRQLDAQEKHLEHAQANILAELSRTKLLLTEHDSALRLAARGTRIDLALPRDAV
                     KASSATAALAATVSRMNWRRGFGGHEGPVNSAAFSPDGSRIVTASTDRTARIWDAASG
                     KEIAVLRGHEDTVISAAFSPDGSRIVTASGDRTARIWDAASADEIAVLRGHEDSVLSA
                     AFSPDGS"
BASE COUNT          414 a          858 c          894 g          424 t
ORIGIN      
        1 gctggagcgg cgaggacgac atcttcctcg atttcgaccc cgagcgcggc atcgtcgccg
       61 gccagcgctg ggcgcgggca cttgaagatg cggccacccg ttgcgaggcc gtcttgttcc
      121 tcgtatcaga ggtttggctc gcctcgaaat ggtgtggcga cgaataccaa ctcgccaaca
      181 ggctcaacaa gaagctgttc gcactgctga tcgatgacat atcgctcgac cgcctgccgg
      241 gtgggctgac cgcgcaatgg caggtggtgc ccctcaaggg agagccggcc gagcgcttcg
      301 tcacggttca ccctcgcacg cagcatcagt ctcccgtcca catagcggag gcggcactta
      361 agagcctcaa gcgaggcctc gagaaggccg gcatcggccc ggagaccttc gagcttcagc
      421 cggatgcgac gggcccattc ggctggcggg cgccctatcg tggcctcgag gcgctcgagc
      481 ccgaggacgc agccgtcttt ttcgggcgca gtgccgatat cgtgcgtggc atcgacgcgc
      541 tgcgcgggct ggctgcgcgc aagtcgccgc gcctgctcgt cgttctcggc gcatcaggtg
      601 ccggcaagtc ctcgtatctg ggcgcggggc tttggccgcg acttctccgc gacgacagtc
      661 aatggctgcc actgcgggcg atccgtgccg gtcgcggcgg tgcaatcgaa ggcagcgaag
      721 ggcttctcca cgcgctcgaa gacgtgcacc gccgcttcgc actgcgggca agtcggtccg
      781 acctgcgcca gcgtctggcg acacccgaat ccttcgtcgc actactgcag gagttgcgtg
      841 gcgccgcggc gcggcgcgcg ctgatctccg cgccgccgca accgctcccg gtgctgtgcc
      901 tcgaccaggg cgaggagctg ttcgcggccg acgccggggc agagagtgaa agctttctac
      961 ggctggcgcg cgctgccgtc gacgcagacg cggcgctgct gctggtcacg atccgctcgg
     1021 atgcctatgg tctcatgcag agcgcaggca cgcttaccgg catcgaccaa gtgcccctaa
     1081 gcctcggccc ggtgccgcaa ggggagattg cccgtattat ccgcgagccg agcgaggtcc
     1141 tacgccgcaa ggccggtccg tccgcgccga tctttgatgc ggccctggta gaacgcctgc
     1201 agagcgaaat cgctggcgag atggacgccc tcccgttgct ggcctttgtg ctgcagcgtc
     1261 tgatgcgcga gcacgcagga acgagcgcga tcggcgtaag ggaactcgac cagaccggtg
     1321 acgtggcggc ggcgatcgaa caggaggcgg aggccgcgct ggccgatgcc gtgtttgggc
     1381 ccgatcgggc cgagcggcgc gaggtgctgc ggcggctgtt cgtcccgcgg cttgcgcgca
     1441 tcgaccgcgc cagcaaggcg ccgcagcgtc gggtcgcgcg ccagagcgat ttccccgtcg
     1501 atttgctggc gctcgcccgc gcgctcaccc agcgtcgtct gttggttacg aagcttgcga
     1561 gcgcggacac gacggagagt gacgcgggga catttgaagc aggtgctgcg acgctagaag
     1621 tggcgcatga ggcgttgctg cgccgctggc cgaccctagc cgacctgctg cgggaagatc
     1681 gcgatgccct gatcctgctc gatggtgtgc tgatcgccgc caccgattgg gacaaggccg
     1741 atgcggcgcg caggtcggat ttcctcgccc accggggatc acgcctggtc gacgcccagg
     1801 cgctcgcgtc acgcggcccc gagtgggcgc gtgaaatcgc cccggcaggg ccttatctcg
     1861 ctgcatgcgc cctacgcgaa atcgaggagc gtaaagaaaa ggaggatgcc ctcgcacgcg
     1921 aacagaaccg cctcgccgag attgcgaccg cccaggagcg taccgggcga ttgcagcggc
     1981 tcacacgttg ggcttttgct gcggtgggag cggtcgtagt aatcgcggtt gtgattgtgg
     2041 gatggcttca gtgggataaa ggacggcaac tcgacgcgca agaaaaacat ctggagcacg
     2101 cacaagccaa cattctcgct gagctgtctc gcacaaaatt gctacttacc gaacatgaca
     2161 gcgcgctgag gctcgcggcg cgcggcaccc ggatcgacct cgcgttgccg cgggatgcag
     2221 tcaaggcttc gtcggccacc gcggcgctcg cggccacggt ctcgcggatg aattggcgcc
     2281 gtggcttcgg cgggcatgag ggccccgtga attccgccgc cttcagcccc gacgggtcgc
     2341 gcatcgtcac ggcgtcaacg gacaggaccg cccgcatctg ggatgccgcc agcggcaagg
     2401 agatcgcggt cctgcgcggc catgaggaca ccgtaatttc cgccgccttc agccccgacg
     2461 ggtcgcgcat cgtcacggcg tcgggggaca ggaccgcccg catctgggac gccgccagcg
     2521 ccgatgagat cgcggtcctg cgcggtcatg aggactccgt gctttccgcc gccttcagcc
     2581 ccgacgggtc
//
LOCUS       DASPXW010000250         4906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_325051, whole genome shotgun sequence.
ACCESSION   DASPXW010000250 DASPXW010000000
VERSION     DASPXW010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4906
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325051"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /locus_tag="VEL04_05790"
     CDS             <1..1155
                     /locus_tag="VEL04_05790"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYT47912.1"
                     /translation="CVAALQLARAQDAPVVVVGAVVSQSGPHADLAAGYRKALLLWQD
                     EVNAAGGLLGRRVELRLLDDGSAAARCAPLYAQLIRDEKAELLIGPYGSAATRVAAAE
                     AERQRRVMINGAGPAHLVHKGATRYLFQSGAPYAAQGAGVLELARAQGLRTLFILARD
                     ELASREMAEAARAAALGLGFTAGEVTAYSGSTTDFTAQVAKAQAAQADAWIAFGEVRD
                     AAEMVKTFKRAGYAPRLFFARKATDPAFVAAVGQDAEFSLGAVEYDPRLASAANSGFA
                     KAFAAKWSAQPGPGAAEGYAAATVLAEAVRRAGSFDQEKLRAALASLETDTVLGSYRV
                     DAASGEQVGIKPAVAQIQRGRPEVVWPNALQTAQPLLPYPQWGERRLLKN"
     gene            1165..2058
                     /locus_tag="VEL04_05795"
     CDS             1165..2058
                     /locus_tag="VEL04_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HYT47913.1"
                     /translation="MTLNRSFLFAPGNVARRVEKALTLHADAVILDLEDSVAPSDKPA
                     TRKLVAEALARPRECRGYVRVNAASTLFCFRDLVETLHKGVDGVVLPKVESAADLHAI
                     DWLIANLEREHGVAEGSIDLMPLIETAAGVQRVERILQARSLRPYAAPWRVKRIAFGA
                     GDYANDLGLAPTLEESELAEARARLVLASRAAGLENPVDSPWFHFKEADAFRRALERS
                     RRGGFQGRLCVHPEQIGPVNGAYLPTEDELAAAERIVAAFRDSEAKGEAAIQVDGQMI
                     DYPIVHRARRLLEAMRKAKAR"
     gene            2055..2672
                     /locus_tag="VEL04_05800"
     CDS             2055..2672
                     /locus_tag="VEL04_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYT47914.1"
                     /translation="MTRDVILAPGLWMPGAAMALLASRLGRAGYAPHLFAYRGRSPFE
                     ANVERMARFAREFLGGRAAHFVGHSLGGVLMLEMLNRSPEVPVASAVLLGAPVRGCIA
                     GRRLGQASLGRWMMGACGPLWEPRSATWKRAAPLGVVAGTVPLGLGRTLGRLPGDNDG
                     VVCVEETAVDGMTARALVAQGHSMLPVSGSVGKLVERFLGTGRFE"
     gene            2669..3664
                     /locus_tag="VEL04_05805"
     CDS             2669..3664
                     /locus_tag="VEL04_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HYT47915.1"
                     /translation="MKLRLAIAVLLTALAAGCESISYYTQAIGGQLNLLARARPIDAW
                     LADPSTPSALRSRLGQAQRIREFASRSLGLPENGSYHAYAELGRPYVVWNVYAAPEFS
                     VEPKQECFPFAGCVSYRGFFSEQDARQHAAQLRSQGYDVHVAGVAAYSTLGWFDDPLL
                     STFVQYSDTQLARLVFHELAHQRVYARNDTTFNESFAVAVEEEGVRRWLEAEGRGAEL
                     DAFRASQTRRREFAARVKQARERLASLYGLGLPRETTLEHKRGEFERLRADYGAIVPA
                     EPNNAFLVSVSLYTDLVPAFERLLAASRGDLPKFYQAVKDLSKSPKDERKLALAR"
     gene            complement(3661..4281)
                     /locus_tag="VEL04_05810"
     CDS             complement(3661..4281)
                     /locus_tag="VEL04_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004566118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HYT47916.1"
                     /translation="MLKIWGRTNSVNVKKALWALEELGVKYERIDAGMQFGVVKTAEY
                     RKLNPNSLVPTIEDDGFVLWESHAIVRYLAAKHGAGTLWPADLRVRADADRWMDWTHS
                     FAREFQRPVFWPMARTPPEQRDMKAIEEACRKCGELLAIPDQVLSNKSFLAGATFTMG
                     DIPLGCHVQLWMRLPIERPRQPNLEAWFNRLCRRPAFKKIVDIAIS"
     gene            complement(4281..>4906)
                     /locus_tag="VEL04_05815"
     CDS             complement(4281..>4906)
                     /locus_tag="VEL04_05815"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016453261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HYT47917.1"
                     /translation="REYLKSRARLVDMKRAQDFGHGTPPKSGTVYLAAADASGMMISF
                     IQSNYMGFGSGVVVDGISMQNRGATFVFAPGHPNRVGPGKRPYQTIIPGFVTKDRRPV
                     MSFGVMGGTMQPQGHAQVMVRIADYGQSPQAASDGPRFRIVQGMDVSVETSFAPAVLD
                     DLVRRGHRTVSLADDYNQFGCAQLIWKLDGGYFAASDPRRDGQAVGF"
BASE COUNT          715 a         1676 c         1722 g          793 t
ORIGIN      
        1 tgcgtcgccg cgctgcagct cgcccgggcg caagacgccc ccgtggtcgt ggtgggcgcc
       61 gtggtatccc agtcgggacc gcacgcggat cttgccgccg gttaccgcaa ggcgctgctc
      121 ctctggcagg acgaagtcaa cgcggccggg gggcttctcg gcagaagggt ggaattgcgg
      181 ctgctcgacg acggttctgc ggcggcgcgc tgcgcgccgc tttacgcgca actcatccgc
      241 gacgaaaagg cggagctcct gatcgggccc tatggctcgg cggcgacccg ggtggcggcc
      301 gccgaggctg agcggcagcg gcgcgtgatg atcaacggcg ctggcccggc ccatctggtc
      361 cacaaaggcg cgacgcgcta cctttttcag agcggcgcgc cgtacgctgc gcaaggcgcg
      421 ggcgtactcg agctggcgcg ggcgcagggc ctcaggaccc tcttcatcct cgcgcgcgac
      481 gagctggcga gccgcgagat ggccgaggcg gcgcgcgcgg ccgctctcgg cctgggcttc
      541 accgcaggcg aggtcacggc ctattccgga tcgacaaccg atttcactgc gcaagtggcg
      601 aaggcgcaag ccgcccaggc cgacgcctgg atcgcctttg gagaggtacg cgacgccgcc
      661 gagatggtca agaccttcaa gcgcgcgggg tatgcgccgc ggctgttttt cgcgcgcaag
      721 gcgaccgatc cggccttcgt cgccgccgtc ggtcaggacg ccgagttcag cctcggcgct
      781 gtcgagtacg atccgcgcct ggcgagcgcc gcgaactcgg gcttcgccaa ggcgttcgcc
      841 gcgaaatggt cggcgcagcc cgggcccggc gccgccgagg gttacgccgc cgcgacggtg
      901 ctcgccgaag ccgtgcggcg cgcgggctcg ttcgaccagg agaagctgcg cgcggcgctt
      961 gccagcctcg agaccgacac cgtgctcggg tcctacagag tggacgcggc gagcggcgag
     1021 caggtcggca tcaagcccgc ggtggcgcag atccagcggg gccggccgga agtggtgtgg
     1081 ccgaacgcgc tgcagaccgc gcagcccctg ctgccttatc cgcaatgggg tgagcggcgc
     1141 ctgctgaaga actgaatcgc tgagatgaca ctgaaccgtt cattcctgtt tgcacccggc
     1201 aacgtggcgc ggcgcgtgga aaaggcgctc acactgcacg ccgacgccgt gatcctcgac
     1261 ctcgaggact cggttgcgcc gtccgacaag cccgcgacgc gcaagctcgt cgccgaggcg
     1321 ctggcgcggc cgcgcgagtg ccgcggctat gtccgggtga atgccgcttc gaccctgttc
     1381 tgcttccgcg atctggtcga gacgctgcac aagggggtcg acggcgtggt gctgccgaag
     1441 gtcgagagcg cggccgacct gcacgcgatc gactggctga tcgccaatct cgagcgcgag
     1501 catggcgtgg ccgagggctc gatcgacttg atgccgctca tcgagaccgc cgccggggtg
     1561 cagcgcgtcg agcggatcct gcaggcgcgc agcctgcggc cctacgcggc tccctggcgc
     1621 gtcaagcgca tcgcgttcgg cgccggcgat tacgcgaacg acctcggcct cgcgccgacg
     1681 ctcgaggaga gcgagcttgc cgaagcgcgc gcgcgcctgg tgctcgcctc gcgcgcagcc
     1741 ggcctggaaa atcccgtcga cagcccctgg ttccacttca aggaagccga tgccttcagg
     1801 cgcgcgctcg agcgcagccg ccgcggcggc ttccagggca ggctgtgcgt gcacccggag
     1861 cagatcggcc cggtgaacgg cgcctacctc ccgaccgagg acgagctcgc ggctgccgag
     1921 cgcatcgtcg cggcgttccg cgactcggag gcgaagggcg aagcggcgat ccaggtcgac
     1981 ggccagatga tcgactaccc catcgtccac cgcgcccggc ggcttctcga ggcgatgcgc
     2041 aaggccaagg caaggtgacg cgtgacgtca tcctcgcccc gggcctgtgg atgcctggcg
     2101 cggccatggc gctgctcgcc tcccggctcg gccgggcggg ctacgcgccg cacctgtttg
     2161 cctatcgtgg caggagcccg ttcgaggcca atgtggagcg catggcgcgc ttcgcgcgcg
     2221 agttcctcgg cggccgcgcg gcgcatttcg tcggccacag tctgggcggc gtgctgatgc
     2281 tcgagatgct gaatcgcagc cccgaggtgc cggtcgcttc ggcggtgctg ctgggggccc
     2341 cggtgcgcgg ctgcattgcc ggccgcaggc tcgggcaggc gagcctcgga cgctggatga
     2401 tgggcgcctg cgggccgctg tgggagccgc gcagcgcgac ttggaagcgc gcggcgccgc
     2461 tcggcgtggt cgccggcacg gttcccctgg gtctcggacg cacgcttggc cggttgccag
     2521 gcgataacga cggcgtggtg tgcgtcgagg agaccgcggt ggacgggatg accgcgcgtg
     2581 cgctcgtggc ccagggccac agcatgctgc cggtctcggg ctcggtcggg aaactggtcg
     2641 agcgtttcct cggcacgggc agattcgaat gaagctgcgg ctcgccatcg ccgtgctgct
     2701 taccgcgctc gccgcgggtt gcgagtcgat ctcctattac acccaggcga tcggcgggca
     2761 gctcaacctg ctcgctcgcg cgcggccgat cgacgcctgg ctcgccgatc cgtccacccc
     2821 gtcagcgctg cgcagccgcc ttgggcaggc gcagcgcatc cgcgaattcg cctcgcgcag
     2881 cctgggcctg cccgagaacg gcagctatca tgcctacgcc gagctcggcc ggccctacgt
     2941 ggtgtggaac gtgtatgccg cacctgaatt ctcggtcgag ccgaagcagg aatgctttcc
     3001 ctttgcgggc tgcgtcagct atcgcggctt tttttccgag caggacgcgc gccagcatgc
     3061 tgcgcaattg cgctcgcagg gctacgacgt gcacgtcgcc ggcgtagcgg cgtattcgac
     3121 cctggggtgg ttcgacgacc cgctgctgtc caccttcgtt cagtactccg acacgcagct
     3181 cgcgcggctg gtgttccacg agctcgccca tcagcgggtc tatgcgcgca acgacaccac
     3241 cttcaacgag tccttcgccg tagccgtcga ggaggagggc gtgcggcgct ggctggaggc
     3301 cgagggacgc ggcgccgagc tcgatgcctt ccgcgcctcg cagacgaggc ggcgggagtt
     3361 cgcggcccgg gtgaagcagg cgcgcgagcg gctggcctcg ctgtacggct tgggactgcc
     3421 gcgcgagacc acgctcgagc acaagcgcgg cgaattcgag cgtctgcgcg ccgattacgg
     3481 cgcgatcgtg ccggcagagc cgaacaacgc tttcctcgtc tcggtgagcc tctataccga
     3541 tctcgtgccg gcgttcgagc ggctgctcgc ggcttcgcgc ggcgacctgc cgaagttcta
     3601 ccaggcggtc aaggacctgt ccaagtcgcc caaggacgag cgcaagctcg cgctggcgcg
     3661 ctagctgatc gctatatcga cgattttctt gaaggccggc cggcggcaga ggcgattgaa
     3721 ccacgcctcc agatttggct gccgcgggcg ctcgatcggc agccgcatcc acagctgcac
     3781 gtggcagcca agcgggatat cgcccatggt gaaggtggct cctgcaagaa aggatttgtt
     3841 tgaaaggacc tgatccggga tggcgagcag ctcgccgcac ttcctgcatg cctcctcgat
     3901 cgccttcatg tcgcgctgct ccggtggtgt gcgcgccatg ggccagaaca ccggacgctg
     3961 gaactctctc gcgaagctgt gcgtccagtc catccagcgg tcggcatccg cacgcacgcg
     4021 caggtcggcg ggccacagcg tgccggcgcc gtgcttcgcc gcgaggtagc gcacgatggc
     4081 gtgcgattcc cacagcacga agccatcatc ctcgatcgtc ggcaccaggg aattgggatt
     4141 gagtttcctg tattccgcgg ttttcaccac gccgaactgc atgccggcgt cgatgcgctc
     4201 gtacttgacg cccagctcct cgagggccca cagcgccttc ttcacattca ccgaattggt
     4261 acgaccccag atcttcagca tcagaagccc accgcctggc cgtcccgccg cgggtcgctg
     4321 gcggcgaagt agccgccgtc gagcttccag atgagctgcg cacagccgaa ctggttgtag
     4381 tcgtccgcga gcgagacggt cctgtggccg cggcgcacga ggtcatcgag caccgccggc
     4441 gcgaagctgg tctcgacgct tacatccatg ccctgcacga tgcggaagcg cgggccgtcg
     4501 ctcgccgcct gcgggctctg cccgtagtcg gcgatgcgca ccatcacctg cgcgtggccc
     4561 tgcggctgca tcgtgcctcc catcacgccg aagctcatca cgggccggcg atccttggtg
     4621 acgaagccgg ggatgatggt ctggtagggg cgcttgcccg gtcccacccg gttcgggtgg
     4681 ccgggggcga agacgaaggt ggcgccgcgg ttctgcatcg agatgccgtc gaccaccacg
     4741 cccgagccga agcccatgta gttcgattgg atgaaggaga tcatcattcc cgaggcgtcg
     4801 gcggcggcga ggtacaccgt gccgcttttc ggcggcgtgc cgtggccgaa gtcctgcgcg
     4861 cgcttcatgt ccacaagcct tgcccttgat ttcaaatatt ccctgt
//
LOCUS       DASPXW010000251         2125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1085090, whole genome shotgun sequence.
ACCESSION   DASPXW010000251 DASPXW010000000
VERSION     DASPXW010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1085090"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VEL04_05820"
     CDS             complement(<1..560)
                     /locus_tag="VEL04_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYT47918.1"
                     /translation="MHSNLRFAPAKVDLEKRADGSLILRSPQKLGPYARCVTEWLSQW
                     SDRTPDGVFLAERKHDGWRKLSYRETYGDVRRIGQALLDLGLGKERPVAILSDNSIDH
                     ALLALGAMHVGVPVAPVSPAYSLMSKDFGKLKYIFELVQPGLVYASDPEKFKLALAAV
                     GAESTSVAKLLESNPGSTMEREFLKIR"
     gene            573..1394
                     /locus_tag="VEL04_05825"
     CDS             573..1394
                     /locus_tag="VEL04_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HYT47919.1"
                     /translation="MEIRRIAGALGAEINGVDLSRPLSDEALSKIRNAWLEHLVIFFR
                     DQRLSPEQFMAFARRIGRPIEYPFVKGIAGFPEIIEVKKLEHEKVNFGGIWHSDTAYL
                     DEPPMASMLLAREVPPAGGDTLFANMYLAYEALSEGMKRLLDGLFAVNDSAKANVTRT
                     REDRIRADGGEASKQFIAEHPVVRTHPETGRKALYVNVGHTVRLRGMTEEESAPLLDF
                     LFRHQIKPEFTCRFAWRPGSIALWDNRCAQHNPVNDYHGYRRVMHRITLAGDKPS"
     gene            complement(1387..2037)
                     /locus_tag="VEL04_05830"
     CDS             complement(1387..2037)
                     /locus_tag="VEL04_05830"
                     /inference="COORDINATES: protein motif:HMM:NF018595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HYT47920.1"
                     /translation="MPDKPKIACLSNGPYYLLNDTTPTPVANLRRANGEACATVRGVA
                     LCRCGASKNKPFCDGSHGTIGFKDVKTTDGSQDRRVTYAGKRITIHDNRHICSHAGIC
                     TDRLQSVFRFRQEPWIDPDGASVQEIIATVEQCPSGALSYSIDGVEHRDRDRPPAVTV
                     TDHGPYAVQGGIELLGVQFGEHASREHYTLCRCGGSKNKPFCDGSHWEIGFRDPVS"
BASE COUNT          366 a          679 c          704 g          376 t
ORIGIN      
        1 cgtattttca gaaactccct ttccatcgtc gagcccggat tgctctcgag cagctttgcg
       61 acggaggtcg attcggcgcc gaccgcggcg agggcgagct tgaacttctc cgggtcggag
      121 gcgtagacca gccccggctg caccagctcg aagatgtact tgagcttgcc gaagtccttc
      181 gacatcaggg aatacgccgg cgagaccggc gccaccggca cgccgacgtg catcgcgccg
      241 agcgcgagca gggcgtggtc gatgctgttg tcggagagga tcgcgaccgg ccgctccttg
      301 cccaggccga ggtcgagcag cgcctggccg atcctgcgca cgtcgccgta ggtctcgcgg
      361 tagctgagct tcctccagcc gtcgtgcttt ctttcggcga gaaacacgcc gtccggagtc
      421 ctgtcggacc actgcgacag ccattcggtg acgcagcgcg cgtacggccc gagcttctga
      481 ggcgagcgca ggatcaggct gccgtcggcg cgcttctcga ggtcgacttt cgcgggcgcg
      541 aagcgcaggt tcgaatgcat aatttgcatt gtatggaaat aagacgcatc gccggcgcgc
      601 tcggcgcgga aattaacggg gttgacctgt cacgccccct ttcggatgaa gctctctcga
      661 aaattcgcaa tgcctggctc gagcacctgg tgatcttctt ccgcgaccag cggcttagcc
      721 cagagcaatt catggccttc gcgcgacgca tcggcaggcc gatcgagtat cccttcgtca
      781 agggcatcgc gggcttcccg gagatcatcg aggtgaagaa gctcgagcac gagaaggtca
      841 atttcggcgg catctggcac tcggacacgg cttacctcga cgagccgccg atggcctcga
      901 tgctgctcgc gcgcgaggtg ccaccggcgg gcggcgacac gctgttcgcc aacatgtatc
      961 ttgcctacga agcgctttca gagggcatga agcgcctcct cgatgggctg tttgcggtga
     1021 atgactctgc aaaggccaac gtcacgcgca cgcgcgagga ccgcatccgc gcggacggcg
     1081 gcgaggcgag caagcaattc atcgcggagc acccggtggt gcgcacgcat ccagagaccg
     1141 gacgcaaggc gctctacgtc aacgtcggcc acaccgtgcg cttgcgcggc atgaccgagg
     1201 aggagagcgc gccgctgctc gacttcctct tccggcacca gatcaagcca gaattcacct
     1261 gccgcttcgc ctggcggccc ggctcgatcg cgctatggga caaccgctgc gcgcagcaca
     1321 acccggtcaa cgactaccac ggctaccggc gcgtgatgca ccgcatcacc ctcgcgggcg
     1381 acaagcctag ctgaccggat cccggaagcc gatctcccaa tgcgagccgt cgcagaacgg
     1441 cttgttcttc gagccgccgc agcggcacag ggtgtagtgc tccctggacg cgtgctcgcc
     1501 gaactgcacg ccgaggagct cgatgccgcc ttgcacggcg tagggcccgt gatcggtgac
     1561 ggtgactgcc ggcgggcggt cgcggtcgcg gtgctcgacg ccatcgattg aataactgag
     1621 tgctcccgac gggcattgct cgacggtggc gatgatttcc tggacgctcg cgccatcggg
     1681 atcgatccac ggctcctggc gaaagcggaa taccgactgc aggcggtcgg tgcagatgcc
     1741 ggcgtgcgag cagatgtggc ggttgtcgtg gatcgtgatg cgctttcccg cgtaggtcac
     1801 gcggcgatcc tggctgccgt cggtggtctt cacatccttg aagccgatcg tgccgtggct
     1861 gccgtcgcag aaaggcttgt tcttcgacgc gccgcaacgg cacagggcca cgccgcggac
     1921 cgtggcgcag gcctccccgt tggcgcggcg caaattggca accggcgtcg gggtcgtgtc
     1981 gttcagcagg tagtacggcc cgttgctcag gcaggcgatt ttgggcttgt cgggcatggc
     2041 ggctaaagtg accgggctag gcggaatggc tacggcatct tagcccgaag cgcgaatggc
     2101 gcgagttcga cccaaagcga cctgt
//
LOCUS       DASPXW010000252         2453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1085196, whole genome shotgun sequence.
ACCESSION   DASPXW010000252 DASPXW010000000
VERSION     DASPXW010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1085196"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="VEL04_05835"
     CDS             complement(<1..1178)
                     /locus_tag="VEL04_05835"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acyl-CoA regulator family
                     protein"
                     /protein_id="HYT47921.1"
                     /translation="MSKREPESGAKLFIGPRFRRLRRQLGLSQTQMADELGVSPSYVN
                     LIERNQRPVTAQILLKMAQAYDVDLRELAAADKDRFFAELNEVFSDPLFRQMELPKQE
                     LRDLVELCPDAANAVQRLYRAYVEARRGESMAAARLAGRDDAPSQETNPVERVRDLIE
                     ANRNHFPALETAAETLRDELDAPAHELFRALSERLRERHGILVRILPVDVMRDTLRRY
                     AQHRKQLLLSELLDGAGCVFQAAFQLALAEAKAPIDQIITASGALDDQARRLFRISLA
                     NYFAAAVIMPYGRFHETAESLAYDIALLGQRFGAGFEQVCHRLTTLARPNARGVPFFM
                     LRVDRAGNVSKRFSSGTFPFSKFGGTCPLWNVHATFETPDRLVTQIIELPDGARYFSI
                     A"
     gene            1326..>2453
                     /gene="aceA"
                     /locus_tag="VEL04_05840"
     CDS             1326..>2453
                     /gene="aceA"
                     /locus_tag="VEL04_05840"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HYT47922.1"
                     /translation="MPKLTFAELMPDAPAGRFDGIERGYSPADVLRLAGSFRTRHSLA
                     ERGANRLWELLQSEAYVPALGAMTGNQAVQMVRAGLNAIYLSGWQVAADSNTAGATYP
                     DQSLYPANSGPELCRRINQALRRADQIEHAEGGIRRDWYAPIVADAEAGFGGPLNAYE
                     VMRAYIEAGVAGVHFEDQLASEKKCGHMGGKVLIPTAAHERNLTAARLAADVCRVPTL
                     VIARTDAESAKLITSDVDERDREFITGERTVEGFFRLKPGSGLAHCIKRGLAFARVAD
                     MLWWETSHPNLDEARTFAEAVHREFPGKLLAYNCSPSFNWRANLDEATIARFQRELGA
                     MGYKFQFVTLAGFHSLNHGMFELARGYAARGMSAYCELQEAK"
BASE COUNT          417 a          788 c          858 g          390 t
ORIGIN      
        1 gcgatcgaga aatagcgcgc gccatccggc agctcgatga tctgggtgac caggcggtcg
       61 ggcgtctcga aggtcgcgtg cacgttccac aacggacagg tgccgccgaa cttcgagaac
      121 ggaaaggtgc cggacgagaa gcgcttcgac acgttgccgg cgcgatcgac gcgcagcatg
      181 aagaacggca cgccgcgcgc gttcggccgg gccagggtgg tgagacggtg gcagacctgc
      241 tcgaagccgg cgccgaagcg ctggcctaaa agcgcgatgt cgtaggccag gctttccgca
      301 gtctcgtgga aacggccgta gggcatgatc acggcggcgg caaagtaatt cgcgagcgag
      361 atgcgaaaga ggcggcgggc ctggtcgtcg agcgcaccgc tcgcggtgat gatctggtcg
      421 atcggcgcct tggcctcggc gagcgccaac tgaaaagcgg cctgaaagac gcagccggcg
      481 ccgtcgagca gctccgacag cagcagctgc ttgcggtgct gggcgtagcg gcgcagcgtg
      541 tcgcgcatca cgtcgaccgg cagtatgcgg accaggatgc cgtggcgctc gcgcagccgt
      601 tccgacagcg cgcgaaacag ctcatgggcc ggcgcatcga gctcgtcgcg cagcgtctcg
      661 gccgcggtct cgagcgccgg gaagtggttg cgattggctt cgatcaggtc gcgcacgcgc
      721 tcgaccgggt tggtctcctg gctcggcgca tcgtcgcggc cggcgagccg cgccgccgcc
      781 atgctctcgc cgcggcgcgc ctcgacatag gcgcggtaga gccgctgcac cgcgttggcc
      841 gcatcggggc agagctcgac caggtcgcgc agctcctgct tgggcaattc catctggcga
      901 aacagcggat cgctgaacac ctcgttcagc tcggcgaaga agcggtcctt gtcggcggcg
      961 gcgagctcgc gcaggtcgac gtcataggcc tgcgccatct tgagcaggat ctgcgcggtg
     1021 accgggcgct ggttgcgctc aatcagattg acatagctcg gcgagacgcc gagctcgtcg
     1081 gccatttgcg tctgcgacag cccgagctgg cggcggaggc gccgaaagcg cgggccgatg
     1141 aacagcttgg cgccggattc gggttcccgt tttgacattt gtgacatcca tacagcttta
     1201 tttgtcacga tgttacagga tagcacgatt gcctggcaat cggcattccc aatcattccg
     1261 cacgaagtta taggtctgag tcaagcgacg acaccatttg tcacgactgt atgaaaggga
     1321 ctgagatgcc gaagctgacg ttcgccgagc tgatgccgga tgccccggcg ggccgtttcg
     1381 acggtatcga gcgcggctat tcgccggccg acgtgctgcg gctcgcgggc tcgttccgga
     1441 cgcgccattc gctcgccgag cgcggcgcca accggctgtg ggagcttttg cagtcggagg
     1501 cctatgtgcc ggcgctgggc gcgatgaccg gcaatcaggc ggtgcagatg gtgcgcgccg
     1561 gcctcaatgc gatctatctc tcgggctggc aggtggcggc cgacagcaac accgccggcg
     1621 cgacctatcc ggaccagagc ctctacccgg cgaattccgg gccggaactg tgccggcgca
     1681 tcaaccaggc gctgcggcgc gccgaccaga tcgagcatgc cgagggcggc atccggcgcg
     1741 actggtacgc gccgatcgtg gccgacgcgg aagccggctt cggcgggccg ctcaacgcct
     1801 acgaggtcat gcgcgcctat atcgaggcgg gcgtcgccgg cgtgcacttc gaggaccagc
     1861 tcgcctcgga gaagaaatgc ggccacatgg gcggcaaggt gctgatcccg accgccgcgc
     1921 acgagcgcaa ccttacggcg gcgcgcctgg ccgccgacgt gtgccgggtg ccgacgctgg
     1981 tgatcgcgcg caccgacgcc gaaagcgcga agctgatcac ctccgacgtc gacgaacgcg
     2041 accgcgaatt catcaccggc gagcgcacgg tcgagggatt tttccggctc aagccgggca
     2101 gcgggctcgc ccactgcatc aagcgcgggc tcgccttcgc gcgcgtggcc gacatgctgt
     2161 ggtgggagac ctcgcatccc aacctcgatg aggcgcggac atttgcggag gcggtgcatc
     2221 gggaatttcc gggcaagctt ctcgcctaca actgctcgcc ctcgttcaac tggcgcgcca
     2281 atctcgacga agcgacgatc gcgcgcttcc agcgcgagct cggcgccatg gggtacaagt
     2341 tccagttcgt cacgctggcg ggcttccaca gcctcaatca cggcatgttc gagctcgcgc
     2401 gcggctacgc ggcgcgcggc atgagcgcct attgcgagct gcaggaggcg aaa
//
LOCUS       DASPXW010000253         2270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_829399, whole genome shotgun sequence.
ACCESSION   DASPXW010000253 DASPXW010000000
VERSION     DASPXW010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829399"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="VEL04_05845"
     CDS             complement(<1..856)
                     /locus_tag="VEL04_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3382 domain-containing protein"
                     /protein_id="HYT47923.1"
                     /translation="MPAEAHRIDFARAVKDAALAGLLAFGLFLPLIGFKTVQNMRNEL
                     ELETRWPLLLTFVAIVAGGRFLHALVVAPWLARRAMRPQAASARAAALRAAFAKWFTP
                     FALGFVAVYPVLVLALVGFGGAVKWIDNFGIQILIYVMLGWGLNIVVGLAGLLDLGYV
                     AFYAVGAYAYALLAKTFGLSFWLLLPLAGILAAFWGILLGFPVLRLRGDYLAIVTLAF
                     GEIVRLVLINWVPVTNGYAGISGIPRPTFFGIPFNASEEGFAARFGLEFSPVYRTIFL
                     YYLILGLAL"
     gene            complement(858..1775)
                     /locus_tag="VEL04_05850"
     CDS             complement(858..1775)
                     /locus_tag="VEL04_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007749150.1"
                     /note="LivHMGF is the membrane component of the LIV-I/LS
                     branched-chain amino acid transporter; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease LivH"
                     /protein_id="HYT47924.1"
                     /translation="MEVFVQQLINGITLGSIYGLIAIGYTMVFGIIGMVNFAHGDVFM
                     VSAFIALIAFLILTAWLGIGSIALALFIVLIVAMALTSLVNWTIERLAYRPLRGSFRL
                     APLISAIGMSIFLMNFVQVTQGPRNKSIPPIVQGEFVLIDSPVYAVTLSYKQLIIWLI
                     TAVLLAAFWYLVAKTSLGRAQRACEQDQRMAALLGIDVDRTISLTFVIGAALAAVAGT
                     MYLMHYGVVNFADGFVPGVKAFTAAVLGGIGSLPGAVLGGLLIGLIETLWSAYFSIDY
                     KDVAAFSILAITLIFLPQGILGRPEVEKV"
BASE COUNT          463 a          726 c          767 g          314 t
ORIGIN      
        1 ccagcgcgag gccgaggatc aggtagtaca ggaagatggt gcgatacacc gggctgaact
       61 cgagcccgaa ccgtgcggcg aagccttctt ccgacgcgtt gaacgggatg ccgaagaagg
      121 tcggccgcgg aatgccgctg atgccggcat agccgttggt gaccggcacc cagttgatca
      181 ggaccaggcg gacgatctcg ccgaaggcca gcgtcacgat ggcgaggtag tcgccgcgca
      241 gcctgagcac cggaaagccc agcaggatgc cccagaacgc ggcgaggatg ccggcgagcg
      301 gcagcagcag ccagaatgac aggccgaacg tcttggcgag cagcgcgtag gcataggcgc
      361 cgaccgcata gaacgcgacg tagccgagat cgagcagccc ggcgagcccg accacgatgt
      421 tgagacccca gccgagcatc acgtagatga gaatttgaat cccgaagttg tcgatccatt
      481 tcaccgcgcc gccgaaacca accagcgcca gcaccagcac cggatagacc gcgacgaatc
      541 cgagcgcgaa cggcgtgaac cacttggcga aggcggcgcg cagcgcggcc gcgcgagcgc
      601 ttgcggcctg cgggcgcatg gcccgccggg caagccacgg cgcgacgacg agcgcatgca
      661 ggaacctgcc gcctgcaaca atagcgacga acgtcaggag cagcggccag cgcgtctcga
      721 gctcgagctc gttgcgcatg ttctgcacgg tcttgaagcc gatgagcggc aggaacaggc
      781 cgaacgccag gaggcccgcg agcgcggcgt ccttcaccgc gcgggcgaaa tcgatgcggt
      841 gtgcctcggc gggcacctca caccttctcg acttccggcc gccccaagat gccctgcggc
      901 aggaagatca acgtgatggc gaggatcgaa aaggcggcga cgtccttgta atcgatggag
      961 aaataggccg accacagggt ctcgatcagc ccgatcagaa gtccgcccag caccgcgccc
     1021 ggcagcgagc cgatgccgcc gagcaccgcc gcggtgaaag ccttgacgcc cggcacgaaa
     1081 ccgtccgcga agttcaccac gccgtagtgc atcagataca tggtgccggc gaccgccgcg
     1141 agcgccgcgc cgatcacgaa ggtgagcgag atggtgcgat cgacgtcgat gccgagcagc
     1201 gccgccatcc tctggtcctg ctcgcaggcg cgctgcgccc ggccgagcga ggtcttcgcc
     1261 acgaggtacc agaaggcggc gagaagcacc gcggtgatga gccagatgat gagctgcttg
     1321 tagctcagcg taacggcata gaccgggctg tcgatcagca cgaactcgcc ctgcacgatc
     1381 ggcgggatcg acttgttgcg cggcccctgg gtcacctgga cgaaattcat gaggaaaatc
     1441 gacatgccga tcgccgagat cagcggtgcc aggcggaacg agccgcgcag cggacggtag
     1501 gcgaggcgct cgatcgtcca attgaccagg gaggtgagcg ccatggccac gatgagcacg
     1561 atgaacaggg cgagcgcgat cgagccgatg ccgagccagg cggtgagaat gaggaaggcg
     1621 atgagcgcga tgaaggcgga caccatgaat acgtcgccgt gggcgaaatt caccatgccg
     1681 atgatgccga acaccatcgt gtagccgatc gcgatcaggc cgtagatcga gccgagcgtg
     1741 atgccgttga tgagctgctg gacgaagact tccatggcgt ccgattcgcc ttcgcggtct
     1801 ttagaacgac tcgtgtctta acaggcgtag ccgtcgggca gcaatggcgc gggcggcgga
     1861 cgtcgaccgt catcctgagg cggccgcgtc cttcgaccgt caccctcatc ctgaggagcc
     1921 cgcgaagcgg gcgtctcgaa ggatgagggt gacggctcac catgacggcg gccctcgaag
     1981 gatggcggcc ccgcccgggc cgtcgcccac cgatctcggg tttacccgag atcggcacca
     2041 tagcgcgcaa gtcggcaaca gccgacttgc gatggctcgc ttcgcgagcg cctcagagcc
     2101 tgccccggac ttgatccggg ggtgacggga atgagtcagt gctctccgtc ccggcacgtc
     2161 ccttgtgtcg accagtttta gggcaccgtg tcctcatgtt gcgtcgaccc gcggcgctgt
     2221 tacactcccc gcgatcgtga acacacgagc cgcagccgtc gccggccgcg
//
LOCUS       DASPXW010000254         5491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_76901, whole genome shotgun sequence.
ACCESSION   DASPXW010000254 DASPXW010000000
VERSION     DASPXW010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5491
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76901"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VEL04_05855"
     CDS             <1..555
                     /locus_tag="VEL04_05855"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HYT47925.1"
                     /translation="DATCPAIHTALQATEKGVPFMPLRGVLGSDLIAHRPDWSVMDNP
                     MRKTGTDPILIVPAIQPDIALFHARWADEAGNVWIGRRRELATIAHAAKRTYVTFEEM
                     KPGDMLEDELLAPGVVSSIYVTATAPAARGAWPLGVAGLYGIDDAHLARYAKAAKTRA
                     GFQRYLDEFVWSGKKSSLPARSRA"
     gene            564..1190
                     /locus_tag="VEL04_05860"
     CDS             564..1190
                     /locus_tag="VEL04_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47926.1"
                     /translation="MPDAARHVAVGAASPIPATGAFLAKQRSPALRISLLQKRAGNPF
                     TEGSRELFDLAGQGRIDVFFLGGVQIDGEANINLVRAEGRRFPGSFGSAFMYSVVKKT
                     ILFREEHSRRVLVPKVEFVSARGDPAALLTGKALFSWQKDRRRWLLESVHESVEIRAE
                     TGFDFDSPAEVPLTPGLSEEDLRLLRGPVARAIAGDYPDFAKRVWGIN"
     gene            1195..2091
                     /locus_tag="VEL04_05865"
     CDS             1195..2091
                     /locus_tag="VEL04_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HYT47927.1"
                     /translation="MNAPEKLKYDRATQDVGNLVHLEHFNCCIDDQRLAVLFYVVGLG
                     ATRDPYIFPGMENIWLNFGRTQVHMPSRAVPPKAERLRGTAGFVVPSLDELKKRLEHA
                     GKEMKRVIPERTTHFDWKEKDGAIEATDPWGTRVRCHAPAPEYGPTDLGLVYVDFDVP
                     PGTAEGIARFYTEVMEAPARAAEARAVVRVGKNQKLLFTETKAPQPEYDGHHIQVYIA
                     DFSTPYKWLKARDLINMETDEAEWRFQWIVDPRDGSKLFQIEHEVRSLKHRLFARPLV
                     NRNHSVTNMTYMTGQDAFRGTY"
     gene            2078..3550
                     /locus_tag="VEL04_05870"
     CDS             2078..3550
                     /locus_tag="VEL04_05870"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HYT47928.1"
                     /translation="MGHTEKGGFWNVYDRPHADLREAIQRFEDAGELLRIRGAHWNLE
                     MGTLAELVYRQDQPPAIVFEDIPGYAKGFRAISGSTNSAKRLAIMLGFPVPSHPLDVV
                     RAYRDRMKLHQPIPPRVVKHGPVRENILSGKEVNVLKFPAPLLHEHDGGRYIGTDDLV
                     IMRDPEENWVNCGTYRSMVQGPDQVGLWISPGKHGRQIRDKYFKQGKPCPVLISCGHD
                     PLLFLAGGNEIRYGLSEYDYAAGHRGLPYDVIESERYGLPMPAHAEIVLEGAMHPGEV
                     LPEGPFGEFTGYYAGGQSPQPVVRVERIYHRNDPILGLACPMVPPSDFSYSKCVMKAG
                     MIWDEVERAGLSGVRGVWVHEAGCARMFNVISIKQAYAGHAKQAALLAASCQSGSYLG
                     RFVVVVDEDVDPANLEEVIWAMSTRCDPVQDIDFVRRHWSGPLDPRLPKGVSWNSRAV
                     IDACRPFEMLADFPPVVKASKALRESTAVKFADQLGRKKP"
     gene            3547..4407
                     /locus_tag="VEL04_05875"
     CDS             3547..4407
                     /locus_tag="VEL04_05875"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HYT47929.1"
                     /translation="MTRATANWIVRASLAGVLTAGPAFAQQRAATEAEARYYIQGAFL
                     TQAAPGIISERVTIGHELRRRLALPPDADKVKIYAALVALTDNKQLVVRKATPEEIAN
                     YPGRARIDPSHPLYTLEAGDIRLLIQYDLQANTVPFIGQLGVPSAEPELAQAGPEPKK
                     PAVVSLVSTELFEFNQATLTQEARAKLDSEVIAKLKDIAEIRYANVNGHADRLGSAQY
                     NQRLSEKRAEAVRAYLVSRGADAAKIETFGFGKTLPVKSCPDRGSRAALIECLAPNRR
                     AQVEVQGTLR"
     gene            complement(4411..>5491)
                     /gene="glpK"
                     /locus_tag="VEL04_05880"
     CDS             complement(4411..>5491)
                     /gene="glpK"
                     /locus_tag="VEL04_05880"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HYT47930.1"
                     /translation="TKIAWLLDNVTGARARAEAGQLAFGTVDSWLIWHLTGNRTHVTD
                     VTNASRTLLFNIHTGDWDPELLQLLRVPRALLPDVHHSAHAFGMLPAGVLGEPLVIGG
                     IAGDQQAAMFGQACHKAGMAKNTYGTGCFMLLHTGSKAAVSSSGLISTACAQIRDKEY
                     ALEGSVFIGGAVVQWLRDELGFFKSSKEIERLAASVLDSGDVYLVPAFTGLGAPYWDA
                     RARGAIIGLTRGTSRAHIARAALESIAFQSAELLEAMQKDAGERLTELRVDGGAAAND
                     LLMQFQADLLGVPVLRPKVLETTALGAAYLAGLTVDLWKSRGEIAAHWKLERRFEPRM
                     ERAEARGKMARWREAVSRSRNWASA"
BASE COUNT          976 a         1836 c         1798 g          881 t
ORIGIN      
        1 gacgccacct gcccggcgat ccacaccgcg ctgcaggcga ccgagaaagg cgttcccttc
       61 atgccgttgc gaggggttct gggttcggat ttgatcgcgc atcgacccga ctggtccgtg
      121 atggacaacc ccatgagaaa aacagggacc gacccgatct tgattgtccc cgcgatccag
      181 cccgacatcg cgctgttcca cgcgcgctgg gcggacgaag cgggcaatgt ctggatcggc
      241 aggcgacgcg agcttgccac gatcgctcac gcggcgaaac gaacctacgt tacgttcgag
      301 gaaatgaagc cgggcgacat gctcgaggac gagctgcttg ctcccggggt ggtttcctcc
      361 atttatgtca ccgccacggc gcccgcggcg cgcggcgcct ggccgctcgg cgtcgccggg
      421 ctgtacggca tcgacgacgc gcatctcgcg cgctatgcga aagcggcgaa gacgcgcgca
      481 ggctttcagc gctacctgga cgaattcgtc tggtcaggaa agaagagctc cttgcctgcg
      541 cgatcgcgcg cctgatcgcg cggttgccag atgcggcgcg ccacgtcgcc gtcggcgcgg
      601 cctcgccgat tccggctacg ggtgcttttc tcgcaaaaca gagaagtccc gccctgcgca
      661 tttcgctgct gcaaaaaaga gccggaaacc ccttcaccga aggctcgcgc gagctgttcg
      721 atctcgccgg ccagggccgc atcgatgtct tcttcctcgg cggcgtgcag atcgacggcg
      781 aagcgaacat caacctggtg cgcgccgagg ggcgccggtt tccgggatcc ttcggctccg
      841 cttttatgta ttctgttgtc aaaaaaacca ttctcttccg cgaagagcac tccaggcgag
      901 ttttggttcc gaaagtcgag ttcgtctcgg cgcgcggcga ccccgcggcg ctgctcaccg
      961 gcaaggcgct tttttcctgg cagaaggaca ggcgccgctg gcttctcgaa agcgtccacg
     1021 agtcggtgga gattcgcgcc gagaccggtt tcgatttcga ttcgccggcg gaggtaccgc
     1081 tcactccggg cctctcagaa gaggatttgc gccttctgcg cggccccgtt gccagggcca
     1141 ttgccggcga ctacccggat ttcgcgaaac gagtatgggg aataaactaa cgccatgaac
     1201 gcccctgaaa agctgaagta cgaccgcgcg acgcaggacg tcggcaacct tgtccatctc
     1261 gagcacttca actgctgcat cgacgaccag cgcctggcgg tgctcttcta cgtggtcggt
     1321 cttggcgcca cgcgcgaccc gtacatcttc cccggcatgg agaacatctg gctcaacttc
     1381 ggtcgcacgc aagtgcacat gccgagccgg gcggtgccgc ccaaggccga gcgcctgcgc
     1441 ggcacggcgg gcttcgtcgt gccgagcctc gacgagttga agaagcgtct ggagcacgcg
     1501 ggcaaggaaa tgaaacgcgt catccccgaa agaacaacgc atttcgactg gaaggaaaag
     1561 gacggcgcga tcgaagccac cgacccgtgg ggcacccggg tgcgctgcca cgcgcctgcg
     1621 ccggaatacg ggcccacgga cctcggtctg gtctacgtgg acttcgacgt gccgcccggc
     1681 accgccgagg gcatcgcgcg tttctacacg gaagtcatgg aggcgcccgc aagggctgcc
     1741 gaggccaggg ccgtggtccg ggtcggaaag aaccagaagc tgctgttcac ggaaacgaaa
     1801 gcgccgcagc ccgagtatga cggccaccac atccaggtct acatcgccga cttctcgacg
     1861 ccctataagt ggctgaaagc ccgcgacctg atcaacatgg agaccgacga ggccgagtgg
     1921 cgcttccagt ggatcgtcga cccgcgcgac ggcagcaagc tgttccagat cgagcacgag
     1981 gtgcgcagcc tgaagcatcg cctgttcgcg cggccgctcg tcaaccgcaa ccactcggtc
     2041 accaacatga cgtacatgac cgggcaggac gcgtttcgtg ggacatactg agaagggcgg
     2101 tttctggaac gtctacgatc ggccccacgc cgacctgcgc gaagcgatcc agcgatttga
     2161 agacgcgggc gagctgctgc gcatccgcgg cgcgcactgg aacctcgaga tgggcacgct
     2221 cgccgagctg gtctaccggc aggaccagcc gccggcgatc gtgttcgagg acatccccgg
     2281 atatgcgaaa ggctttcgcg ccatttccgg ctcgaccaat tcggcgaagc ggctcgcgat
     2341 catgctcggc ttcccggtgc cctcgcaccc gctcgacgtg gtgcgcgcct accgcgaccg
     2401 catgaagctg caccagccga tcccgcctcg cgtggtcaag catggacctg tcagggaaaa
     2461 catcctcagc ggaaaagaag taaacgtcct caagtttccc gcacccctgc tgcacgagca
     2521 cgacggcggc cgctatatcg gcaccgacga tctcgtgatc atgcgcgatc ccgaggagaa
     2581 ctgggtcaac tgcggcacct atcgctcgat ggtgcagggg cccgaccagg tcggcctgtg
     2641 gatctcgccg ggcaagcacg gccgccagat ccgcgacaaa tacttcaagc agggcaaacc
     2701 ctgcccggtg ctcatcagct gcgggcacga cccgctgttg tttctcgccg gtggcaacga
     2761 gatccgctac ggtctctcgg agtacgacta cgccgccggc caccgcggcc tgccgtacga
     2821 tgtcatcgag tccgagcgct acgggctgcc gatgccggcg cacgccgaga tcgtgctcga
     2881 aggcgcgatg catccaggcg aggtgctgcc cgaagggccg ttcggcgaat tcaccggcta
     2941 ctacgcgggg gggcagagcc cgcagccggt ggtgcgcgtc gagcgcatct atcaccgcaa
     3001 tgacccgatt ctcgggctgg cgtgcccgat ggtgccgcct tccgactttt cgtattcgaa
     3061 gtgcgtcatg aaggccggca tgatctggga cgaagtcgag cgcgccgggc tctcgggcgt
     3121 gaggggagtg tgggtgcacg aagccggctg cgcgcgcatg ttcaacgtga tctcgatcaa
     3181 gcaggcttac gctgggcacg ccaagcaggc ggcgctgctc gccgcgagct gccagtcggg
     3241 gtcctacctc gggcgcttcg tggtggtggt cgacgaagac gtcgacccgg ccaacctgga
     3301 ggaagtgatc tgggcgatga gcacgcgctg cgacccggtg caggacatcg acttcgtccg
     3361 ccgccactgg agcgggccgc tcgatccgcg gctgccgaaa ggcgtgagct ggaactcgcg
     3421 tgccgtgatc gacgcctgcc ggccgttcga aatgctcgcg gatttcccgc cggtcgttaa
     3481 ggcatcgaag gcgctgagag aaagcaccgc ggtgaagttt gccgaccagc tcgggagaaa
     3541 gaagccatga ctcgagcgac ggcgaactgg atcgtccgcg catcgctggc tggagtactg
     3601 acggcgggcc ccgctttcgc tcagcagcgg gctgcgaccg aagcggaggc tcgttactat
     3661 atccagggcg cctttctgac gcaggcggcg cccggcatca tcagcgagcg cgtgacgatc
     3721 ggacacgagc tgcgccggcg gctcgccctg ccgcccgacg ccgacaaggt caagatctac
     3781 gcggcgctgg tggcgctcac cgacaacaag cagctggtcg tgcgcaaagc tacgcccgag
     3841 gagatcgcga attaccccgg gcgggcccgc atcgacccga gccatccgct gtacacgctg
     3901 gaggccggcg acatcaggct gctcatccag tacgacctgc aggcgaacac ggtgccgttc
     3961 atcggtcagc tcggcgtgcc ctccgcagag ccggagctgg cgcaagccgg gcccgagccg
     4021 aagaagcccg ccgtcgtcag cctcgtctcg accgagttgt tcgagttcaa ccaggcgacg
     4081 ctcacgcagg aggcgcgcgc caagctcgac agcgaggtga ttgccaagct gaaggacatc
     4141 gccgagatcc gctacgccaa cgtcaacgga catgccgacc ggctgggctc ggcgcaatac
     4201 aaccagaggc tctcggagaa gcgcgccgaa gcggtgcgcg cctacctggt gtccaggggc
     4261 gccgatgcgg cgaagatcga gaccttcggc ttcggcaaga ccctgccggt gaagtcatgc
     4321 cctgaccggg gcagccgcgc cgcgctgatc gagtgccttg cgcctaaccg cagggcgcag
     4381 gtcgaagtcc agggcaccct caggtaacgc tcaggcgctc gcccagttcc gcgagcggct
     4441 gaccgcctcg cgccagcgcg ccatcttccc ccgcgcctcg gcgcgctcca tcctcggctc
     4501 gaagcggcgc tcgagcttcc agtgcgccgc gatctcgccg cgcgacttcc acagatcgac
     4561 cgtcaggccg gcgaggtagg cggcgccgag cgcggtcgtc tccagcactt tcggccgcag
     4621 caccggcacg ccgagcaggt cggcctggaa ctgcatcagg agatcgttgg ccgccgcgcc
     4681 gccgtccacg cgcagctcgg tgagccgctc gccggcatcc ttctgcatcg cctcgagcag
     4741 ctcggcgctc tggaaggcga tcgattccag cgccgcccgt gcaatatggg cgcgcgacgt
     4801 gccgcgagtc aggccgatga tcgcgccgcg cgcccgcgcg tcccagtacg gcgcgcccag
     4861 gccggtgaag gcgggaacca gatagacgtc gccgctgtcg agcacgctgg ccgccagtct
     4921 ttcgatctct tttgagctct tgaagaaacc gagctcatcc cgcagccact gcaccaccgc
     4981 gccgccgatg aacacgctgc cttcgagggc atattctttg tcccggattt gtgcgcacgc
     5041 tgtcgagatc aatccactgg aagaaacagc ggctttgctc cccgtgtgca gcagcatgaa
     5101 gcagccggtg ccgtaggtgt tcttcgccat cccggccttg tggcaggcct ggccgaacat
     5161 cgcggcctgc tggtcgccgg caatgccgcc gatcaccagc ggctcgccga gcacgcccgc
     5221 gggcagcatg ccgaaggcgt gcgccgaatg atgcacgtcg ggcagcagcg cgcgcggcac
     5281 gcgcaacagc tgcagcagct ccgggtccca gtcgcccgtg tggatgttga agagcagggt
     5341 gcgcgaggcg ttcgtcacgt cggtgacgtg cgtgcgattg ccggtgaggt gccagatgag
     5401 ccagctgtcg acggtgccga aggcgagctg gcccgcttcc gcgcgggcgc gcgcgccggt
     5461 cacgttatcg agcagccagg cgatcttggt g
//
LOCUS       DASPXW010000255         2065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1211895, whole genome shotgun sequence.
ACCESSION   DASPXW010000255 DASPXW010000000
VERSION     DASPXW010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211895"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..1324
                     /locus_tag="VEL04_05885"
     CDS             11..1324
                     /locus_tag="VEL04_05885"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HYT47931.1"
                     /translation="MSDRHQVIIVGGGAGGLELATRLGDRLGRKGRAAITLVDRNETH
                     LWKPLLHEVAAGSMDIHAHQLDYLAQARWHHFSFRCGAFEGLDRTRREITVAPTMDER
                     GEEVIPRRTLRYDTLVIAIGSVANSFGVPGVAEHAFALETAAEADRFHRHLINACSQA
                     NYRPKGSAPAQLRLVIVGGGATGVELAAELHNTTRVFAEYGLENIDPERFLHVTLIDA
                     GPRILPMLPERIAKAVTETLEGLGVRVVGSEQAVEVTASEVKTKSGKSFPADIAVWAA
                     GIRGADILKDLDGLETNRINQLVILPTLQTTRDPDIFAIGDCSVLPREGRMPPVPPAA
                     QAAHQQASHMVDTIERRLRGEPSRPFRYRDFGSLVSLGRYSTIGSLMGFLSGKSYRVE
                     GWFARLMYLSLYRMHLYALYGPLGVALDTLARLLKRGTEPRVKLH"
     gene            1324..>2065
                     /locus_tag="VEL04_05890"
     CDS             1324..>2065
                     /locus_tag="VEL04_05890"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYT47932.1"
                     /translation="MLASLVKIALAVAIGVPLLAWLFQERMIFFPRPVESRPTPRPNV
                     EDVALAAADGVKLRGWLVRGAGTTAPLVIYFGGNAEEVSWLTEVADQFSGWSLLLVNF
                     RGYGESEGKPGEKELLQDGLAIYDYVKRRPDVIPERIVAMGRSLGSGVAVHLAAHRPL
                     RGVILVSPYDSIVEVAKRHYPFLPVSLLLRHRFDSLALAPQVDAPLLCLVATEDRVVP
                     IPHSRALFEAWRGAKTWREVPRAGHDSIS"
BASE COUNT          320 a          705 c          706 g          334 t
ORIGIN      
        1 tgaactttcc atgagcgatc ggcatcaggt catcatcgtc ggcggcgggg cggggggcct
       61 ggagctcgcc acgcgcctcg gcgaccggct cggacgcaag ggccgcgccg cgatcacgct
      121 ggtcgaccgg aacgagacgc acctgtggaa gcccctgctg cacgaggtcg ccgcgggcag
      181 catggacatc cacgcgcacc agctcgacta cctcgcgcag gcgcgctggc atcatttcag
      241 cttccgctgc ggcgccttcg aaggcctcga tcgcacgcgg cgcgaaatca cggttgcgcc
      301 gaccatggac gagcggggcg aagaagtcat cccgcgccgc acgctgcgat acgacacgct
      361 ggtgatcgcg atcggcagcg tcgcgaacag cttcggcgtt cccggggtcg ccgagcacgc
      421 tttcgcgctc gagaccgcgg ccgaagccga ccgcttccac cggcacctca tcaacgcctg
      481 cagccaggcg aactaccggc cgaagggcag cgcgccggcg caactgcgct tggtgatcgt
      541 cggcggcggc gcgaccggcg tggagctcgc cgccgagctg cacaacacca cgcgcgtgtt
      601 cgccgaatac ggcctcgaga acatcgaccc ggagcggttt ctccacgtca cgctcatcga
      661 cgccggcccg cgcatcctgc cgatgctgcc cgagcgcatc gcaaaggcgg tgaccgagac
      721 cctggaagga ctgggcgtgc gggtggtggg ctcggagcag gcggtcgaag tgactgcgag
      781 cgaagtgaag accaagagcg gcaagtcctt tcccgccgat atcgcggtct gggcggccgg
      841 gatcagggga gccgatatcc tcaaggatct cgacggcctg gagaccaacc gcatcaacca
      901 gctggtgatc ctgccgacgc tgcagacgac gcgcgacccg gacatcttcg cgatcggcga
      961 ttgctcggtg cttccgcgcg aggggaggat gccgcctgtg ccgcccgcgg cacaggccgc
     1021 gcaccagcag gcctcgcaca tggtggatac gatcgagcgc aggctgcggg gggagccgtc
     1081 gcggccattc cgctatcggg acttcggctc gctcgtatcg ctcggccggt acagcaccat
     1141 cggatctctg atgggttttc tctcgggcaa gagctaccgc gtcgagggct ggttcgcgcg
     1201 gctcatgtac ctgtcgttgt acaggatgca cctgtacgcg ctgtacggac ccttgggcgt
     1261 cgcgctcgat acgctcgcgc ggctcctgaa gcgcggcacc gagccgcggg tcaagctgca
     1321 ctgatgctcg cctcgctcgt caagatcgcc cttgcggtgg ccatcggcgt gccgctgctc
     1381 gcgtggctct tccaggagcg gatgattttc tttccgaggc cggtcgaatc ccgcccgacc
     1441 ccgcgtccga acgtcgagga cgtggccctt gccgccgcgg acggcgtaaa gctgcgcggc
     1501 tggctggtga ggggcgccgg cacgaccgcg ccgcttgtca tctacttcgg cggcaacgcg
     1561 gaggaggtct cctggctcac cgaggtcgca gaccagttct ccggctggtc gctgctgctc
     1621 gtgaattttc gcggctacgg cgaaagcgag ggcaagcccg gcgaaaagga gctcctgcag
     1681 gacgggctcg cgatttacga ctatgtgaag cggcgccccg acgtgattcc cgaacgcatc
     1741 gtcgcgatgg gacgaagcct ggggagcggc gtcgccgttc acctcgccgc tcatcgcccc
     1801 ttgcgcggcg tgatactcgt gtcgccctac gacagcatcg tcgaagtggc gaagcggcac
     1861 tatccctttc tgccggtgtc gctgctgctg cggcaccgct tcgactcgct tgccctcgcg
     1921 ccgcaggtcg atgcgcccct cctgtgcctg gtggcgacgg aggaccgggt cgttccgatc
     1981 ccgcattcgc gggcgctgtt cgaagcctgg cgcggcgcca agacctggcg ggaggttccc
     2041 cgggccggac acgacagcat ctcgg
//
LOCUS       DASPXW010000256         2603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_326193, whole genome shotgun sequence.
ACCESSION   DASPXW010000256 DASPXW010000000
VERSION     DASPXW010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326193"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="VEL04_05895"
     CDS             complement(<1..669)
                     /locus_tag="VEL04_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYT47933.1"
                     /translation="MSTAIVTGGSAGIGLAVVRQLLQKNYEVISLDVQPAELKHAKLS
                     HVKVDLTDTAATSRVAKEINRKAITTLIHNAGVIRPALLPDVKLEDFDALVNLHLAAA
                     IVLMQAALPAMKQAAFGRVVLVSSRAVLGLATRTSYSATKAGMLGMARTWALELAAFG
                     ITVNVVAPGPIRTANFHSVVPEGSPQVERIAQAVPVRRLGAVDDVARAVMFFADRDAG
                     FVTGQ"
     gene            complement(666..1154)
                     /locus_tag="VEL04_05900"
     CDS             complement(666..1154)
                     /locus_tag="VEL04_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012836537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HYT47934.1"
                     /translation="MTAWRPSDSDLARFVRREARLIDERRFDEWYELFADDGCYWVPA
                     APGQADPLNHNSLAYEDKLLLQLRVERLKSPLAYSQKPASRCLHVLQEPEVEKRDDAS
                     GEYLTRTPFIYTESRGDESQRYAAVAWHTLVPLQERLRIRLKRVDILNCDAALPSIQL
                     FL"
     gene            complement(1151..2440)
                     /locus_tag="VEL04_05905"
     CDS             complement(1151..2440)
                     /locus_tag="VEL04_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HYT47935.1"
                     /translation="MGRYRDDAGALKRLIEDQRVHRDVYTDAEVFQLEMERLWSRTWI
                     YVGHASQVPNSGDFITLDIAAQPVIMVRHTDNKIRVLLNRCAHKGTKVVYDTTGNAGK
                     TFRCPYHAWTYRTDGSLLHVPLPEGYASTVQRNGLTQVKSETYRGFVFARLAKDGIGF
                     RDYFGDSLSSIDNLADRSPEGELEIAGGCLRYLHNCNWKMFVENLNDTMHPMIAHASS
                     AGTAKRLWAGKPAALPKPMAIEQIAPFANDYQFFDDMGVRVYAHGHSFSGVNFSIHSS
                     YSSVPEYEAAMQKAYGEERARTILGTVRHNTVYYPSLTIKGAIQSIRVARPLAADKTL
                     IESWTFRLKGAPDALLQRTVTYNRLINSPMSVVGHDDLHCYRSIQEGLAAQGNEWVNL
                     QRNFDSKEIEQQEITANGTSDISMRNQFRAWREFMLP"
     gene            complement(2407..>2603)
                     /locus_tag="VEL04_05910"
     CDS             complement(2407..>2603)
                     /locus_tag="VEL04_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYT47936.1"
                     /translation="EDRTAPASIMASMQRKIQGSELVVLEGCGHLGPMDQPAAFNAAL
                     ERFLRKMESRWEGIATTPAR"
BASE COUNT          423 a          802 c          900 g          478 t
ORIGIN      
        1 ctgcccggtg acgaagcccg cgtcgcggtc ggcgaagaac attacggcgc gcgcgacatc
       61 atcgacagcc cctagccgcc tgaccgggac ggcctgggcg atgcgctcga cctgagggct
      121 gccctcggga acaacggagt gaaagttggc ggtgcggatc ggccccggcg cgaccacgtt
      181 gaccgtaatg ccgaaggcag cgagctcgag cgcccaggtg cgggccatgc cgagcatgcc
      241 ggccttggtg gcggaatagg aggtgcgggt cgcaaggccg aggaccgcgc gtgaggagac
      301 gagaaccacg cggccgaatg cggcctgctt catggcaggc agcgccgcct gcatgaggac
      361 gatcgccgcc gccaggtgca ggttgaccag cgcgtcgaaa tcctccagct tcacatccgg
      421 gaggagggcg gggcggatga cgccggcgtt gtggatgagg gtggttattg ccttccggtt
      481 gatctccttc gcaacgcgag aggtcgccgc cgtgtcggtg agatccacct tcacatgact
      541 caatttggcg tgcttcaact ccgcgggctg cacgtcgagc gagatcacct catagttttt
      601 ttgcagcaat tgccttacca ccgccaggcc gatgccagcg ctgccgccgg tgacgatcgc
      661 ggtgctcaca ggaagagttg aatcgagggc agcgcggcgt cgcagttgag gatgtcgacg
      721 cgcttcagcc ggatgcgcag ccgctcctgc aatggcacca gcgtgtgcca ggcgacggcg
      781 gcatagcgct gcgactcgtc accccggctc tcggtgtaga tgaatggcgt gcgcgtcagg
      841 tactcgccgc tcgcgtcgtc gcgcttttcc acctcgggct cctgcaggac gtgcaggcag
      901 cggctcgccg gcttctgcga gtaggcgagc gggctcttca gccgctcgac gcgcagctgc
      961 aaaagaagct tgtcctcgta ggcgagcgaa ttgtgattga gcggatcggc ctggccgggg
     1021 gcggccggca cccagtagca gccgtcgtcg gcgaacagct cgtaccactc gtcgaagcgc
     1081 cgctcgtcga tgagccgcgc ctcgcgccgg acgaagcggg cgagatcgct gtcggacgga
     1141 cgccaggccg tcacggcagc atgaactcgc gccaggcgcg gaactggttg cgcatcgaga
     1201 tgtcgctcgt gccgttcgcg gtgatctctt gctgttcaat ttccttacta tcaaagttgc
     1261 gctggaggtt cacccattcg ttgccctgcg ctgccagccc ctcctggatc gagcggtagc
     1321 agtgcaggtc gtcgtggccg acgaccgaca tcggcgagtt gatgaggcgg ttataggtga
     1381 ccgtgcgctg gagcagggcg tcgggcgcgc ccttgaggcg gaaggtccag gattcgatca
     1441 gggtcttgtc ggcggcgagc ggccgcgcga cgcggatcga ctggatcgcg cccttgatgg
     1501 tcaggctcgg gtagtacacc gtgttgtggc gcaccgtccc gaggatcgtg cgggcgcgct
     1561 cctcgccata ggccttctgc atcgccgcct cgtattccgg cacgctcgaa taggacgagt
     1621 ggatcgagaa gttgacgccg gagaagctgt ggccgtgggc gtagacgcgc acgcccatgt
     1681 cgtcgaagaa ctggtagtcg ttggcgaaag gcgcgatctg ctcgatcgcc atcggcttgg
     1741 gcagcgccgc gggcttgccc gcccagagcc gcttcgcggt gcccgcggac gaggcatgcg
     1801 cgatcatcgg atgcatggtg tcgttcaggt tctcgacgaa catcttccag ttgcagttgt
     1861 gcaggtagcg caggcagccg ccggcgatct cgagctcgcc ttcgggcgag cggtcggcga
     1921 ggttgtcgat cgaggacagc gaatcgccga agtagtcgcg gaagccgatg ccgtccttcg
     1981 cgagccgagc gaagacgaag cctcgatagg tttcgctttt gacctgggta aggccgttgc
     2041 gctgaaccgt gcttgcatat ccttccggga gcggtacatg gaggagggaa ccgtccgtac
     2101 ggtaagtcca cgcgtgatac gggcagcgga acgtcttccc cgcgttgccc gttgtgtcgt
     2161 agacgacctt cgttcctttg tgcgcgcagc ggtttaggag cacgcggatc ttgttgtccg
     2221 tatgccggac catgatcacc ggctgcgcag cgatgtcgag cgtgatgaaa tcgccggagt
     2281 tcggcacctg gctcgcgtgg ccgacgtaga tccaggtgcg cgaccacagg cgctccatct
     2341 cgagctggaa cacctcggcg tcggtgtaga cgtcgcggtg cacgcgctgg tcctcgatca
     2401 ggcgcttcag cgcgccggcg tcgtcgcgat accttcccat cggctttcca ttttgcgcaa
     2461 aaaccgctct agcgccgcat tgaaggcggc aggctggtcc attggcccga gatggccgca
     2521 gccttcgagc accaccagct cgctgccctg gatcttgcgc tgcatgctcg ccatgatcga
     2581 agccggcgcg gtgcggtcct ccg
//
LOCUS       DASPXW010000257         2481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_201248, whole genome shotgun sequence.
ACCESSION   DASPXW010000257 DASPXW010000000
VERSION     DASPXW010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201248"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VEL04_05915"
     CDS             <1..260
                     /locus_tag="VEL04_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HYT47937.1"
                     /translation="VGVPVLVDAGVGTASDAAIAMELGCDGVLMNTAIAAAREPVLMA
                     RAMKKAVEAGREAFLAGRMAKKLYTAAPSSPTEGTISSKAA"
     gene            286..960
                     /gene="trmB"
                     /locus_tag="VEL04_05920"
     CDS             286..960
                     /gene="trmB"
                     /locus_tag="VEL04_05920"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018911647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HYT47938.1"
                     /translation="MVRPIRSYVLRQGRITPAQTRALEKLYPKYGLAFVPHAISSAEV
                     FNRKAPLVLEIGSGMGETTTEIASAHPETDFIAVEVHGPGVGSLLNAIENNQLTNVRV
                     IRHDAVEVLEHMIPDGALAALHLFFPDPWPKKRHHKRRLVQAEFAALTARKLAPGGAL
                     HAATDLPDYAEHMNSVFMQEPLFDPANTGFVERPTTKFETRGKRLGNPIRDLFFRRRE
                     TARAAR"
     gene            complement(908..2092)
                     /gene="hemW"
                     /locus_tag="VEL04_05925"
     CDS             complement(908..2092)
                     /gene="hemW"
                     /locus_tag="VEL04_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HYT47939.1"
                     /translation="MNQIVLERPGAVRLKVLPPLALYVHIPWCVRKCPYCDFNSHQRG
                     GALPEREYVERLFLDLESLLPAVWGRRLVSVFIGGGTPSLFSPESIDALLSGIRARLP
                     LEPQAEISLEANPGTVEAARFRGFREAGVNRISIGVQSFDDRMLAALGRIHGSEEARR
                     ALDAAAASFDNVNLDLMYGLPGQSAAMARKDIEQALACGVPHVSAYQLTIEPNTVFWS
                     TPPGLPEHDLCADMQVMIEEILGQAGYEHYETSAFARAGRRCRHNLNYWEFGDYLGIG
                     AGAHGKVSFPDRITRHEQVKQPTAYLKGLSIENREIPAGELPFEFMLNALRLIEGFPI
                     VLFAERTGLPITLLEPQLRAAEEQQLIERDWQRIRPSARGRLFLNELLELFLAGERKD
                     RG"
     gene            complement(2073..>2481)
                     /locus_tag="VEL04_05930"
     CDS             complement(2073..>2481)
                     /locus_tag="VEL04_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HYT47940.1"
                     /translation="LADDSGLCVEALGGEPGVRSAYYAGREGSREERDRRNNELLLKN
                     LSTERKAYYYCAMVLVRKPGDPRPLVAEGVWRGEIARAPRGANGFGYDPLFLLPDLGK
                     TAAELDSAQKNRISHRGIALQKLLLSLNESNRS"
BASE COUNT          458 a          819 c          782 g          422 t
ORIGIN      
        1 gcgtcggcgt gccagtgctc gtggacgcgg gtgtcggcac cgcgtcggat gccgccatcg
       61 ccatggaact aggttgcgac ggcgtgctca tgaacaccgc gatcgccgcg gcgcgcgagc
      121 cggtgctgat ggcgcgagcg atgaaaaagg cggtcgaggc cgggcgcgaa gccttcctcg
      181 ccggccgcat ggcgaaaaag ctctacaccg cggcgcccag ctcgccgacc gaagggacga
      241 tctcgtccaa ggccgcctga gccgcggctt gccgcggcgc tcgtcatggt ccggccgatc
      301 agaagctacg tcctgcggca aggcaggatc accccggcgc agacccgcgc ccttgaaaag
      361 ctctatccga aatatggatt ggcatttgtt cctcatgcca tttcgtcggc agaggtcttc
      421 aatcgaaaag cccctctggt gctcgaaatc ggctcgggga tgggcgagac gaccacggaa
      481 atcgcaagcg cgcatcccga aacagatttc atcgccgtcg aggtgcatgg gcccggcgtc
      541 ggcagccttt tgaacgcgat cgaaaacaac cagctcacga acgtgcgcgt catcaggcac
      601 gacgcggtcg aggtgctgga gcacatgatc ccggacggcg cgctcgcggc gctgcatctt
      661 ttctttcccg atccctggcc gaagaagcgc caccacaagc ggcgcctggt gcaggccgaa
      721 ttcgccgcgc tcacggcaag gaagctcgcc cccggcggag cgttgcacgc ggcgaccgac
      781 ttgcccgact acgcggagca catgaattcg gtcttcatgc aggaaccgct cttcgatcca
      841 gcgaacaccg gatttgttga aagaccgacc acgaaattcg aaacccgagg caagcgcctc
      901 ggcaacccta tccgcgatct tttctttcgc cggcgagaaa cagctcgagc agctcgttga
      961 ggaacagccg cccgcgcgcg ctcgggcgga tgcgctgcca gtcgcgctcg atcagctgct
     1021 gttcttccgc cgctctcaac tgcggctcga gcagcgtgat gggcaggccg gtgcgctccg
     1081 caaacaacac gatggggaag ccttcgatca ggcgcaatgc attcagcatg aattcgaaag
     1141 gcagctcgcc tgccgggatt tccctgtttt cgattgaaag tcccttcagg tatgccgtcg
     1201 gctgcttgac ctgctcatgg cgcgtgatgc ggtcggggaa gctcacttta ccgtgggcgc
     1261 ccgcgccgat gccgaggtaa tcgccgaact cccagtagtt gaggttgtgg cggcagcgcc
     1321 ggcccgctcg ggcaaacgcc gaggtctcgt aatgctcgta tccggcttga ccaaggattt
     1381 cttcgatcat tacctgcatg tcggcgcaca ggtcgtgctc cggaagcccg ggcggcgtgc
     1441 tccagaacac cgtgttcggc tcgatggtca gctggtaggc cgaaacatgc ggcacgccgc
     1501 aggcgagcgc ctgctcgatg tccttgcgcg ccatggccgc actctggccg ggaagcccgt
     1561 acatcaggtc gaggttcacg ttgtcgaagg acgcagccgc cgcatcgagc gcgcgccgcg
     1621 cctcctcgct gccgtggatg cggccgagcg ccgccagcat ccggtcatcg aagctctgca
     1681 cgccgatcga gatgcggttc acgccggcct cgcggaagcc gcgaaagcgc gcggcttcga
     1741 cggttcccgg gttcgcctcg aggctgatct cggcctgcgg ctcgagcggc agccgcgccc
     1801 ggatgccgga gagcagcgcg tcgatcgatt cgggcgaaaa caggctcggc gtgccgccgc
     1861 cgatgaacac gctgaccaat cgccgccccc acaccgcagg cagaagactt tccaggtcta
     1921 gaaataatct ctccacatat tcgcgctccg gcaatgcgcc gccgcgctgg tgcgaattga
     1981 agtcgcaata cgggcatttg cgcacgcacc acgggatgtg gacgtagagc gcaagcggcg
     2041 gcagcacctt caggcgaacg gcgcccgggc gctcaagaac gatttgattc attcaacgat
     2101 agcagcaatt tctgcaacgc aatgccgcgg tggctgatgc ggttcttctg cgcagaatcc
     2161 agctcggcag cggttttccc aaggtccggg agaagaaaaa gcggatcgta gccgaagccg
     2221 ttcgcgccgc gcggcgcgcg cgcgatctcg ccgcgccaga cgccctcggc gaccagcggt
     2281 cgcggatcac ctggcttgcg cacgaggacc atggcgcagt agtaatacgc ttttctctct
     2341 gtggaaaggt tctttaagag caattcatta ttcctgcgat cccgctcctc ccggctgccc
     2401 tcgcggcccg cgtaatacgc agaacgcacc ccgggctcgc cgccgagcgc ctcgacgcac
     2461 agcccggaat cgtcggcgag c
//
LOCUS       DASPXW010000258         4827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1086694, whole genome shotgun sequence.
ACCESSION   DASPXW010000258 DASPXW010000000
VERSION     DASPXW010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4827
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086694"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VEL04_05935"
     CDS             <1..245
                     /locus_tag="VEL04_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HYT47941.1"
                     /translation="AAAEVIRDDAGIDAEFSAILRQLVTYMMEDPRTISAALDIIWIA
                     KAIERIGDHAKNMAEQVIYIVKGTDVRHTGPIAVEK"
     gene            242..928
                     /gene="phoB"
                     /locus_tag="VEL04_05940"
     CDS             242..928
                     /gene="phoB"
                     /locus_tag="VEL04_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HYT47942.1"
                     /translation="MSATVLVVEDEPQIQELVAVNLEHLGHRVLRAASAEEAETSIRA
                     ALPDVVVLDWMLPGESGLSFARRLRSDERTRDLPILMLTARAMEQDKVSGLEAGADDY
                     LTKPFSPKELAARIKAVLRRRAPQHSGDAVELEGLRLDPATHRVTAGEHRLALSPSEF
                     RLLHFLLTHPGRVYSRAQLLDQIWGDHVYVEERTVDVHIRRLRKALEPSGHNRLIDTV
                     RGAGYGLRTA"
     gene            925..2238
                     /gene="phoR"
                     /locus_tag="VEL04_05945"
     CDS             925..2238
                     /gene="phoR"
                     /locus_tag="VEL04_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HYT47943.1"
                     /translation="MSLVYRNTLAALAGFGLVALLVGVVAGAAWGWGAFSAGLLAVLL
                     YHVRHLTRLARWVANPVAGKVPEGTGGWDEVLAALHRYERATAKRGEQLAEALTRFRH
                     AAQALPDGVVILDAANRIEWCNPTAEAQLEINGRADIGQPIANLVREPEFIEYVAAGE
                     ASSPIRIARPAGRSFTLQLIPYGQSQKLLLSRDTTQAERLETMRRDFVANVSHELRTP
                     LTVLVGFLETVRELRLDPQRVRDYLGMMREQASRMHRIIEDLLALSVLESAPPPPVER
                     VRVASLLERLRADAEALSGGRHAISLHGSPSVDLAGADSELSSAFSNLVSNAIRYTPA
                     GGKVRLVWRDGPDGASFSVEDTGLGIASEHIPRLTERFYRVDHSRSRESGGTGLGLAI
                     VKHALARHQATLDVESKPGAGSRFTVRFPPQRTIPLEAARQTEAG"
     gene            complement(2260..4392)
                     /locus_tag="VEL04_05950"
     CDS             complement(2260..4392)
                     /locus_tag="VEL04_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HYT47944.1"
                     /translation="MHLEHLNPEQLAAVTLPDEHALILAGAGSGKTRVLTTRIAWLVK
                     GGRVAPAGILAVTFTNKAAREMLTRIAAMLPVNPRGMWIGTFHGLCNRMLRAHHRDAG
                     LPQAFQILDSQDQLAAIKRLCKSLNVDEDRFAPRDLQYFINAQKEEGVRARDVAVTDD
                     HARRLIELYAAYDEQCQREAVVDFPELLLRSFELLSRNQILREHYSRRFRHILVDEFQ
                     DTNRLQYRWLKLFNSEETRYFCVGDDDQSIYTFRGAHVGNMAEFEREFKIARVIRLEQ
                     NYRSQGNILDAANALIANNRNRLGKNLWTARGAGEPVRVFEGQSDMDEARWIVEEVQA
                     LAREGTRLSQAAVLYRSNAQSRVVEHALFSQGIAYRVYGGLRFFERAEVKHALAYLRL
                     VASPDDDNAFLRVANFPPRGIGARTIEQLQDAAKQNRSSLFAAADGKAGVFKKLIEDL
                     RDQTQNLPLPEVIEHVVAKSGLVEHYKTEREGAERIENLEELVNAAAAFTDEAREAES
                     GETVDPLTAFLTHAALEAGEHQAGEGQEALQLMTVHSAKGLEFDVVFLSGLEEGLFPH
                     EQSVMERDGLEEERRLAYVAITRARHRLYLSHAQTRMLHGQTRYNIPSRFLQEIPEAL
                     LKWLTPRFSRRKAFTADFSRTKDAFLMKKPRDTGGFRIGQNVVHPKFGSGVIIDAEGQ
                     GTDARVQVNFGTQGVKWLAVAVAKLQAA"
     gene            complement(4440..>4827)
                     /locus_tag="VEL04_05955"
     CDS             complement(4440..>4827)
                     /locus_tag="VEL04_05955"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HYT47945.1"
                     /translation="EGEQAVKMDQPLQFYRRSEGKTQPVGFIDRKQLDAWASETDLEA
                     GKGAARLGGRWSVWLAAAEDQSSALRVYDELRAAGYPAEILPKKDADKLVYQVRIRQL
                     PSKAEAQALAAQLRGRYGVTEPRVSM"
BASE COUNT          751 a         1697 c         1556 g          823 t
ORIGIN      
        1 ccgccgcggc cgaagtgatc cgcgatgacg ccggcatcga cgccgagttc agcgccatcc
       61 tcaggcagct ggtcacctac atgatggagg acccgcgcac catctccgcc gcgctcgaca
      121 tcatctggat cgccaaggcg atcgagcgta tcggcgatca tgccaagaac atggccgagc
      181 aggtgatcta catcgtcaag ggcaccgacg tgcgccatac cgggcccatc gccgtggaaa
      241 aatgagcgca acggtgctgg tcgtcgagga cgagccgcag atccaggagc tggtggcggt
      301 caacctcgag cacctcggcc accgcgtgct gcgcgccgcg agcgcagagg aagccgagac
      361 ctcgatccgc gccgcgctgc ccgacgtcgt ggtgctcgac tggatgctgc cgggggaatc
      421 ggggctatcg ttcgcgcgcc ggctgcgcag cgacgagcgc acgcgcgacc tgccgatcct
      481 gatgctcacc gcgcgcgcga tggagcagga caaggtctca ggtctcgaag cgggcgcaga
      541 tgactacctc accaagccgt tttccccgaa ggagctggcg gcgcgcatca aggcggtgct
      601 gcgccggcgc gcgccgcagc attcgggcga cgcggtcgag ctcgaaggcc tgcgccttga
      661 tcccgccacg caccgtgtca cggccggcga gcaccggctc gcgctttcgc cttccgagtt
      721 tcgcctgctg cacttcctgc tgacgcatcc cggccgcgtg tattcgcgcg cccagctcct
      781 cgaccagata tggggcgacc acgtctacgt ggaggagcgc accgtcgacg tgcacatccg
      841 ccgcctgcgc aaggcgctcg agccttcggg ccacaaccgc ctgatcgaca cagtgcgcgg
      901 cgccgggtac ggcctgcgaa ccgcttgagc ctggtctacc gcaacacgct cgcggcgctc
      961 gccggtttcg ggctcgtcgc gctgctcgtt ggcgtcgtcg ccggggcggc ctggggctgg
     1021 ggtgcctttt cggccggcct cctggctgtg cttctctatc acgtgcgcca cctgacgcgc
     1081 ctcgcgcgct gggtcgccaa cccggtggcg ggcaaggtgc ccgagggcac cggcggctgg
     1141 gatgaagtgc tcgccgcact gcaccgctac gagcgcgcca ccgcaaagcg cggggagcag
     1201 ctcgccgaag cattgactcg tttccgccac gcagctcagg cacttcccga cggcgtggtg
     1261 atcctcgatg ccgccaaccg catcgaatgg tgcaatccga cggccgaagc ccagctcgag
     1321 atcaacggca gggcggacat cgggcaaccc atcgccaacc tggtccgcga accagaattc
     1381 atcgagtacg ttgcggcggg cgaggcgtcc tcgccgatcc gcatcgcgcg cccagccggc
     1441 cgctccttca cgttgcagct gatcccttat ggccagtcgc agaagctgct tctatcgcgc
     1501 gacaccacgc aggccgagcg cctcgagacc atgcggcgcg acttcgtcgc caacgtctca
     1561 cacgagctgc gcacgccgct tacggtcctg gtcggcttcc tcgagacggt gcgcgagctc
     1621 aggctcgacc cgcagcgcgt gcgcgactac ctgggcatga tgcgcgagca ggcctcgcgc
     1681 atgcaccgca tcatcgagga cctgctcgcg ctttccgtgc tcgaatcggc gccgcctccg
     1741 cccgtcgagc gcgtgcgcgt ggcatcgctc ctcgagcgcc tgcgcgccga cgccgaggcg
     1801 ctctctggcg gccgccatgc aatttccctt catgggagcc caagtgtcga cctcgcaggc
     1861 gccgacagcg agctttcgag cgcgttcagc aacctggtaa gcaacgcgat ccgctacacg
     1921 ccagccggcg gcaaggtccg ccttgtgtgg cgagacggcc ccgacggcgc ctcattctcg
     1981 gtcgaggaca cgggtctcgg catcgcgtcc gagcacattc cgcgtctcac cgagcgcttc
     2041 taccgggtcg atcacagccg ctcgcgcgaa tcgggcggca ctggcctcgg tctcgcgatc
     2101 gtgaagcacg cgctcgcgcg ccatcaggcc acgctcgatg tcgagagcaa acccggtgct
     2161 ggaagccgat ttacggtgcg cttcccaccc caacgcacga ttccgctcga ggctgccagg
     2221 cagactgaag cgggataaag aaggcctcgc gcaccggttc taggcggcct gcagcttggc
     2281 caccgccacg gcgagccact tcacgccctg ggtgccgaaa ttcacctgca cgcgggcatc
     2341 ggtcccctgg ccctcggcat cgatgatcac gcccgagccg aatttcggat gcacgacgtt
     2401 ctgcccgata cggaagccgc cggtatcgcg tggttttttc atcaggaagg catccttggt
     2461 tctggagaaa tcggcagtga aggccttccg ccgggaaaac cgcggcgtca gccatttcag
     2521 caacgcctcc gggatttcct gcagaaagcg cgacgggatg ttgtagcgcg tctggccgtg
     2581 cagcatgcgc gtctgcgcgt gcgagaggta aaggcggtgc cgcgcgcgcg tgatcgccac
     2641 gtaggcgagc cgccgctcct cctccagccc gtcgcgctcc atcacgctct gctcgtgggg
     2701 aaacaggcct tcttccagcc ccgagaggaa caccacgtcg aattcgagcc ctttcgcgga
     2761 atgcaccgtc atcagctgca gcgcctcctg gccctcgccc gcctggtgct cgcccgcctc
     2821 gagcgctgcg tgcgtgagaa aggcggtgag cggatcgacc gtttcgccag actcggcttc
     2881 gcgcgcttcg tcggtgaacg cggccgcggc gttcaccagc tcctccaggt tctcgatccg
     2941 ctccgcgccc tcgcgctcgg tcttgtagtg ctcgacgagg ccgctcttcg ccaccacgtg
     3001 ctcgatcacc tcgggcagcg gcaggttctg cgtttggtcc ctcaggtctt cgatcagctt
     3061 tttgaacacc cccgctttgc cgtcagccgc cgcaaaaagt gagcttcgat tctgttttgc
     3121 cgcatcctga agctgctcga tggtgcgcgc gccgatgccg cgcggcggga aattggcgac
     3181 gcgcaggaac gcgttgtcgt cgtcgggcga ggcgacgagg cgcagatacg ccagcgcgtg
     3241 cttcacctcg gcgcgctcga agaagcgcaa gcccccgtac acccggtagg cgatgccctg
     3301 cgaaaacagc gcatgctcca ccacgcgcga ctgcgcgttc gagcggtaca gcaccgccgc
     3361 ctgcgagagc cgcgtgccct cgcgcgcgag cgcctgcacc tcctcgacga tccagcgcgc
     3421 ctcgtccatg tcggattggc cctcgaacac gcgcaccggc tcgcccgcgc cgcgcgcggt
     3481 ccagaggttc ttgccgagcc ggttgcggtt gttcgcgatc agcgcgttgg ccgcgtcgag
     3541 gatgttgccc tgcgagcggt agttctgctc caggcggatg acgcgcgcga tcttgaactc
     3601 gcgctcgaac tcggccatgt tgccgacatg cgcgccgcgg aaagtgtaga tcgactggtc
     3661 gtcgtccccg acgcagaagt atcttgtttc ctcggaattg aacagcttca gccaccggta
     3721 ctgcaggcgg ttggtgtcct ggaactcgtc gaccaggatg tgccggaaac gtcttgaata
     3781 atgctctcgc agtatctgat ttctgcttag caactcaaaa gagcgcagca gcaattccgg
     3841 aaaatccacc accgcttcgc gctggcactg ctcgtcgtag gcggcgtaga gctcgatgag
     3901 gcggcgcgca tggtcgtcgg tcacggcgac gtcgcgcgcg cgcacgccct cctccttctg
     3961 cgcgttgatg aagtactgca ggtcgcgcgg cgcaaagcgg tcctcgtcga cgttgaggct
     4021 cttgcacagg cgcttgatcg cggcgagctg gtcctgcgag tcgaggatct ggaaagcctg
     4081 cggcaggccc gcgtcacggt ggtgcgcgcg cagcatgcgg ttgcacaggc cgtggaaggt
     4141 accgatccac atgccgcgcg ggttcaccgg cagcatcgcc gcgatgcgcg tcagcatctc
     4201 gcgcgccgcc ttgttggtga aggtcacggc aaggatgccg gcgggggcga cgcggccgcc
     4261 tttcaccagc caggcgatgc gcgtggtcag cacgcgggtc ttgccgctgc ccgcgccggc
     4321 gaggatcagc gcgtgctcgt ccggcagggt gaccgcggcg agctgctcgg gattgaggtg
     4381 ttcgagatgc agcgacaagc cgcgatttta ctgcggcccg catcagctac ctcaggacct
     4441 cacatcgaaa ccctcggctc ggtgacgccg tacctgccgc gcagctgcgc ggcgagcgcc
     4501 tgcgcctcgg ccttcgacgg aagctgccgg atgcgaacct ggtagacgag cttgtcggcg
     4561 tccttcttcg gcaggatctc cgcgggatag ccggcggcgc gcagctcgtc gtacacgcgc
     4621 agcgcgctgc tctggtcttc cgcggctgcc agccatacgc tccagcgccc gccaagccgc
     4681 gccgcgccct tgcccgcctc gagatcggtc tcactcgccc acgcgtcgag ctgctttcga
     4741 tcgataaagc cgaccggctg cgtcttgccc tcgctgcgcc ggtagaactg cagcggctgg
     4801 tccattttca ccgcctgctc gccttcg
//
LOCUS       DASPXW010000259         2933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1714727, whole genome shotgun sequence.
ACCESSION   DASPXW010000259 DASPXW010000000
VERSION     DASPXW010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1714727"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="VEL04_05960"
     CDS             complement(<1..631)
                     /locus_tag="VEL04_05960"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:NF016593.4"
                     /note="with component II, the glutamine amidotransferase,
                     catalyzes the formation of anthranilate from chorismate
                     and glutamine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HYT47946.1"
                     /translation="MTEAEFLKLANAGYTRIPVLAEAGADLYTPLAVYITLAGGPYSY
                     LLESVVGGERFGRYSFIGLACAERIEARGRTLRRLLRDARGAVVCLESLEGVDPFEYV
                     RAWLGRQRVAPAPAALRFGGGLVGYFGYESVRNIEKNALQGEKADALGTPDMLLLVSD
                     ELAVVDNVLGKLYLVVYADPGQPDALRAAKQRLERLRARLARPLPSNEIL"
     gene            complement(777..1442)
                     /gene="rpe"
                     /locus_tag="VEL04_05965"
     CDS             complement(777..1442)
                     /gene="rpe"
                     /locus_tag="VEL04_05965"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HYT47947.1"
                     /translation="MTYRIAPSILSADFARLGDEVRAVAAAGADLVHFDVMDNHYVPN
                     LSVGPLVCAALRPHVKLPLDIHLMVKPVDRLVPDFAKAGANIITFHPEASEHIDRTIA
                     LIREHGCKPGLVLNPATPLSFLDHTLEKLDVVLLMSVNPGFGGQQFLRSVLPKIAQVR
                     RRIDALGREIWLEVDGGIKTDNVAEAARAGADTFVAGSAIFGSKDYAATIRAMREQLK
                     DMR"
     gene            1468..1851
                     /gene="apaG"
                     /locus_tag="VEL04_05970"
     CDS             1468..1851
                     /gene="apaG"
                     /locus_tag="VEL04_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HYT47948.1"
                     /translation="MAQEKQYDVAVSAQTHYLPDQSDETGSRYVFAYTITIRNSGSVA
                     AQLISRHWIITDSQGLVQEVRGLGVVGAQPLLQPGESYEYTSGASIATPVGTMRGSYQ
                     MVAEDGTRFEASIPEFTLSVPRVLH"
     gene            1942..>2933
                     /locus_tag="VEL04_05975"
     CDS             1942..>2933
                     /locus_tag="VEL04_05975"
                     /inference="COORDINATES: protein motif:HMM:NF015523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HYT47949.1"
                     /translation="MTHISRALLALIVLAAGCAQVPPKPEIPVPPPKPAPEAASYRET
                     TFDALPGWGQGPLAPSLRAFLTGCGRPPSLLVLACELAALVPPDDDTAARQFFESAFT
                     PYAMIPSDGGDSGLVTGYYEPIIDGTKSRDADNRFPIYGVPDDLIVVDLATVNPDVRG
                     MRLRGRLEGRRLVPYYSRAEIDSRGAGFQAPVIAWTADPVELFFLHIQGSGQVRLPDG
                     VRLRIGFADQNGHPFRSMGRYLVERGEMSLDQASMQNIKAWAAANPHKLRDALNVNPS
                     YVFFRELQGLKVDEGPIGAMNVPLTAGYSLAVDRRFVPLGSPVYLATNFPLSEER"
BASE COUNT          512 a          987 c          961 g          473 t
ORIGIN      
        1 ctaaaatttc attgctggga agcggccggg cgaggcgcgc gcgcagccgc tcgagccgct
       61 gcttcgccgc acgcagcgca tcgggctgcc ccggatccgc gtacaccacc aggtagagct
      121 tgccgagcac gttgtcgacc acggcgagct cgtctgaaac cagcagcagc atgtccggcg
      181 tgccgagggc atccgctttc tctccctgca aggcattttt ttcaatattt cgcacgctct
      241 cgtagccaaa gtaacccacc agcccgccgc cgaagcgcag cgcggcaggc gcgggcgcga
      301 cgcgctggcg cccgagccag gcgcgcacat actcgaacgg atcgacgccc tcgagactct
      361 ccaggcacac gacggcgccg cgcgcatcgc gcagcagccg ccgcagggtc ctgcctcgcg
      421 cctcgatgcg ctcggcgcag gcgagtccga tgaacgaata gcgcccgaag cgctcgccgc
      481 cgaccaccga ctcgaggagg tacgagtagg ggccgcccgc aagcgtgatg tagaccgcga
      541 gcggcgtgta gaggtcggcg cccgcttcgg cgagcaccgg gatgcgggta tagcccgcgt
      601 ttgcgagctt gaggaattct gcttcagtca cgactctctc cgtcctgaaa ggacgactgc
      661 gggaaaacta ctgcgaaacg acgtgcggac ttagcggcgc cagcgacgcc agaggagaga
      721 gacgaagtgt ccggaggaga gcacgatggg cgaggactat atcacggcgc catgtgctag
      781 cgcatgtcct tcaactgctc gcgcatcgcg cggatcgtcg cggcgtaatc cttcgagccg
      841 aaaatcgccg agccggcgac gaaggtgtcg gcgccggcgc gcgccgcctc ggcgacgttg
      901 tcggtcttga tgccgccgtc gacctcgagc cagatctccc ggcccagcgc atcgatgcgc
      961 cgccgaacct gcgcaatctt gggaagcacc gaacgcagga actgctggcc gccgaacccc
     1021 gggttcaccg acatcagcag cacgacgtcg agcttctcga gcgtatggtc gagaaatgac
     1081 agcggggtcg ccgggttgag caccagccct ggcttgcagc cgtgctcgcg gatgagcgcg
     1141 atggtacggt cgatgtgctc ggaagcttca gggtggaagg tgatgatgtt cgcgccggcc
     1201 ttggcgaaat cgggcaccag gcggtccacc ggcttgacca tcaggtgaat gtcgaggggc
     1261 agcttgacgt gcgggcgcag cgccgcgcag accagcgggc cgacgctgag gttgggcacg
     1321 taatggttgt ccatgacgtc gaagtgcacc aggtcggcgc ccgcggccgc gaccgcgcgc
     1381 acctcgtccc ccagccgggc gaagtccgcg gaaaggatgc tcggcgcaat gcggtaggtc
     1441 atctcgtcgc gagtttacaa tgcgggcatg gcgcaggaga aacagtacga cgtcgcggtc
     1501 agcgcacaga cccattatct ccccgaccag tccgacgaga cgggcagccg ctacgtgttc
     1561 gcctacacca tcacgatccg caactcgggc agcgtggccg cgcagctcat cagcaggcac
     1621 tggatcatca ccgactctca agggctggtg caggaagtcc gcgggctggg cgtggtcggc
     1681 gcgcagccgc tgctgcagcc gggcgagagt tacgagtaca ccagcggcgc ctcgatcgcg
     1741 acgccggtgg gcacgatgcg cggcagctac cagatggtcg ccgaggacgg cacgcggttc
     1801 gaggcgtcga tcccggagtt cacgttgtcg gtgccgcggg tcctgcactg atcacttttc
     1861 tgcattgatt actttttctt cggccaggtc caccacacga tcagcgcggc aagcgcgagt
     1921 accacgccga cttccagata gatgacccac atatcgcgag cgctcctcgc gctgattgta
     1981 cttgccgccg gctgcgcgca agtgccgccg aaaccggaga tcccggtgcc gccgcccaag
     2041 ccggcgcccg aggcagcgag ctaccgggag acaactttcg acgccctgcc aggatggggg
     2101 caagggccgc tcgcgcccag tctgcgcgcg ttcctcaccg gctgcggacg cccgccgtct
     2161 ctgctggtgc ttgcctgcga gctcgcggcg ctggtgccgc cggatgacga tactgccgcg
     2221 aggcaattct tcgaatctgc cttcacgccc tacgccatga ttccttccga cggcggcgac
     2281 agcgggctcg tcaccggcta ttacgaaccg atcatcgacg gcaccaagtc ccgcgacgcc
     2341 gataaccgct tcccgatcta cggcgttccg gacgatctga tcgtggtcga cctcgcgacg
     2401 gtcaatcctg acgtgcgcgg catgcgcttg cgcggaaggc tggagggccg ccggctggtt
     2461 ccctattact ctcgcgccga gatagattca cgtggcgccg gatttcaggc gccggtcatt
     2521 gcctggaccg cggatcccgt cgagctcttc tttctccaca tccagggctc gggacaggta
     2581 cggctgccgg atggcgtgcg gctgcgcata ggcttcgcag accagaacgg ccatcccttc
     2641 cgctcgatgg gccgctatct cgtcgaacgg ggcgagatgt ccctcgacca ggcatcgatg
     2701 cagaacatca aggcttgggc ggcggccaac ccgcacaagc tgcgagacgc gctcaacgtc
     2761 aacccgagct acgtcttctt ccgcgagctg caggggctga aggtcgacga gggcccgatc
     2821 ggcgcgatga acgtgccgct caccgccggc tacagcctcg cggtcgaccg gcgcttcgtg
     2881 ccgctcggct cgccggtgta cctcgccacc aactttccgc tctcggagga gcg
//
LOCUS       DASPXW010000260         4408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_202267, whole genome shotgun sequence.
ACCESSION   DASPXW010000260 DASPXW010000000
VERSION     DASPXW010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4408
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202267"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="VEL04_05980"
     CDS             <1..736
                     /locus_tag="VEL04_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYT47950.1"
                     /translation="GVDAARAGEAWERFGPLMEEAERRGRSFPRKHPIVRPQKHEIEW
                     RANVTQLSNPDGSPVDGTDAESLSWGEIQGRRQVVDFFEFLRGAAPGFEKAYVLDIAP
                     QVGIRETRRITGEYQLSEDDVLSCASFDDTIGVNGWPIESHVRGDVVFRWPQIPESRG
                     FNQLPYRMLLPLRIDNLLVAGRCASMTHMGQSAARVSGACFAMGEAAGIAADLSLASG
                     KRCRDVNVTELQRRLEAGGAFLGRSA"
     gene            733..1701
                     /locus_tag="VEL04_05985"
     CDS             733..1701
                     /locus_tag="VEL04_05985"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYT47951.1"
                     /translation="MNLLVRLCGLLVFTAFADSAAAQAYPSKPVRLIVPFPAGSTPDI
                     VGRVLGQKLSEAWRQPVVIDNKTGAGGNIGTAEAAHAAPDGYTLVIGSNGPIAVNKAL
                     YKDLPYDPDKDLKPISLLAAAPQLLAVHPLVPASDLKQFVAYVRANPDKLSYGSVGAG
                     SASHLTMELLKSQAQLAIVHVPYRGFPPVVQDLLSGQIQVTFAIAPAVLPNIKAGKLK
                     GLAVTSARRSPLAPEIPTVAEQGFAQFDATAWIGLLAPARTENAITEKLAAETQRIMR
                     LPEVRAALGNQGFEVVGSAPEEFASFIRTETAKWTKIVRATGAKAE"
     gene            complement(1679..3205)
                     /locus_tag="VEL04_05990"
     CDS             complement(1679..3205)
                     /locus_tag="VEL04_05990"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HYT47952.1"
                     /translation="MRVEFNRTSVADSTRVLVLHETRLPPMYYFPREDVRLELLGRTE
                     HRTHCPFKGDASYWTLRVDDAVAENAAWSYEEPYDDAAPIRQYLAFYPSRVSAIYDGE
                     DEAPHLGRGASMHANAIAGWLLAEAWKAATPEELMAGFCRCLQEAGVPVSRMTIIIPA
                     LHPQVFATVLVWRDDAGVKTIFEPHDILQQPRFKDSPFALILRGAGGVRRRMERPEVK
                     LDFPVVRDLKAEGASDYVAMPFRFSDGQMNVMSMTSFAAGGFSTDHLGRIFEILPGLA
                     RYFEVYAQRRTAVTLLKTYLGHHTGDRVLAGQVKHGDGEQIHSVVWFSDLRESTMLSK
                     SMPQDAYLAHLNRYFDCMAGAVMEKGGEVLRFIGDAVLAIFPIAEASGRHWASASGAA
                     EACRRAIDAATIACERIAAANRSNPQATPIGFGIGLHLGDVTYGNIGVRERLEFTVIG
                     ASANEAARVESMSKTLKQPVVISAAFARTYPGKLASLGRHALKDVAGEQELFTLPSPR
                     "
     gene            complement(3270..>4408)
                     /locus_tag="VEL04_05995"
     CDS             complement(3270..>4408)
                     /locus_tag="VEL04_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HYT47953.1"
                     /translation="ALLAHPASVTAALVHSLDALAALDDLDVTAAFGPQHGLRGDKQD
                     NMIESSDFTDPVHGIPVFSLYGSVRRPTAQMMDSFDVLLVDLQDVGCRVYTFLTTLRY
                     VLEESARKRKIVWLLDRPNPAGRPVEGLRLRPGWESLVGAGPLPMRHGLTLGEAGKWL
                     IRSLGLDLDYQVVAMQGWAPDAPPGWGWPLGERAWVNPSPNAPNPGMARCYPGTVMLE
                     GTKLSEGRGTTRPLELFGAPDLEPRGLLQKMHSLAPQWMQGCRLRACWFEPTFQKHVG
                     ELCAGLQIHVEDAAYDHRAFRPWRLVALALKALRLLQPDYSLWRDFAYEYEQGRLAID
                     LITGSELLRRWVDDPAAGPADLDAAARLDEEAWREEREAVMLYR"
BASE COUNT          720 a         1520 c         1466 g          702 t
ORIGIN      
        1 cggcgtcgat gcggcgcgcg ccggcgaggc ctgggagcgc ttcgggccgc tcatggaaga
       61 ggccgagcgg cgcggcagga gcttcccgcg caagcatccg atcgtccggc cgcagaagca
      121 cgagatcgaa tggcgcgcca acgtcacgca gctcagcaat cccgacggct cgccggtcga
      181 cggcacggat gccgagagcc tctcctgggg cgagattcag gggcggcgcc aggtggtcga
      241 ttttttcgag ttcctgcgcg gcgccgcgcc gggcttcgag aaagcctacg tgctcgacat
      301 cgcgccgcag gtcggcatcc gcgagacgcg gcgcatcacc ggcgagtacc agctctccga
      361 ggacgacgtg ctttcctgcg cgagcttcga cgacaccatc ggcgtcaacg gctggccgat
      421 cgaatcgcac gtgcgcggcg acgtggtgtt ccgctggccg cagatccccg agtcgcgcgg
      481 cttcaaccag ctgccgtatc gcatgctgct gccgcttcgc atcgacaacc tgctggtcgc
      541 gggccgctgc gcctcgatga cccacatggg gcagtctgcg gcgcgcgtct cgggcgcctg
      601 cttcgccatg ggggaagccg ccggcatcgc ggccgacctc tcgctggcca gcggcaagcg
      661 ctgccgcgac gttaatgtga cggagctgca gcgccggctc gaagcaggtg gtgcttttct
      721 tggcaggtcc gcatgaacct cctggtcaga ctgtgcggac ttctggtttt cacggcattt
      781 gcagacagcg ccgctgcgca ggcctatccg tcgaagccgg tgaggctgat cgtgccgttc
      841 ccagcaggct cgacgcccga catcgtcggc cgcgtgctcg ggcagaagct gagcgaggcc
      901 tggcgccagc cggtggtcat cgacaacaag accggcgccg gcggcaacat cggcacagcg
      961 gaagcagcac acgccgcgcc ggacggctac acccttgtga tcggcagtaa cgggccgatc
     1021 gcggtcaaca aggcgttgta caaggatctg ccttacgacc ccgacaagga tctgaagccg
     1081 atctcgctcc ttgcagccgc gccgcagctg ctcgccgtcc accccttggt gccggcaagc
     1141 gacctgaagc agttcgtggc ctatgtgcgc gccaatcccg acaagctctc ctacggctcg
     1201 gtcggcgcgg gcagcgcctc gcacctgacg atggaactgc tgaagtcgca ggcgcagctc
     1261 gccatcgtgc acgtgcccta ccgcggcttt ccgccggtgg tgcaggacct gctgtcaggg
     1321 caaatccaag tgacgttcgc catcgctccc gccgtgctgc cgaatatcaa ggcgggaaag
     1381 ctgaagggtc tcgcggtgac gagcgccagg cgcagcccat tggcaccgga gatacccacg
     1441 gtggcggagc aaggattcgc gcagttcgac gccacggcct ggatcggctt gctcgcgccg
     1501 gcccgcactg aaaacgccat caccgagaaa ctcgcggccg aaactcaacg catcatgcgc
     1561 ctgccggaag tgcgcgcggc gctgggcaat cagggcttcg aagtggtcgg cagcgcgccc
     1621 gaggagttcg cgagcttcat ccgcaccgag accgcgaagt ggaccaaaat cgtgagagct
     1681 accggggcga aggcagagta aagagctcct gctcgccggc gacgtccttg agcgcgtggc
     1741 gcccaagcga ggccagtttc cccggatagg tcctggcgaa cgcggcggag atcaccactg
     1801 gctgcttgag cgtcttgctc atgctttcga cgcgcgccgc ttcgttggcg gaggcgccga
     1861 tgacggtgaa ctccaggcgc tcgcgcacgc cgatgttgcc gtaggtgacg tcgccgaggt
     1921 gcagcccgat gccgaagccg atcggcgtcg cctgcggatt gctccggttg gcggccgcga
     1981 tccgctcgca ggcgatggtc gcggcgtcga tcgcccggcg gcaggcctcg gccgcgccgc
     2041 tcgccgaggc ccagtgcctg ccgctcgcct cggcgatcgg gaagatcgcg agcaccgcgt
     2101 cgccgatgaa gcgcagcacc tcgccgccct tctccatcac cgcgccggcc atgcaatcga
     2161 agtagcgatt gaggtgcgcg aggtaggcat cctgcggcat cgacttggag agcatggtcg
     2221 actcgcgcag gtccgagaac cacaccacgc tgtggatctg ctcgccgtcg ccgtgcttca
     2281 cctgcccggc gagcacgcgg tcgccggtgt ggtggccgag ataggtcttg agcagcgtca
     2341 ccgcggtgcg ccgctgggcg tacacctcga agtagcgcgc caggccgggc aggatctcga
     2401 agatccgccc gaggtggtcg gtcgagaagc cgcccgcggc gaacgaggtc atcgacatca
     2461 cgttcatctg cccgtccgag aagcggaacg gcatcgccac gtagtcgctc gcgccctccg
     2521 ccttgaggtc gcgcaccacc gggaagtcga gcttcacctc agggcgctcc atgcgcctgc
     2581 gcaccccgcc ggcgccgcgc aggatgagcg cgaacgggct gtccttgaac ctcggctgct
     2641 gcaggatgtc gtgcggctcg aagatcgtct tcacgccggc gtcgtcgcgc cacaccagca
     2701 ccgtggcgaa tacctgagga tgcagcgccg ggatgatgat ggtcatgcgt gagaccggca
     2761 cccctgcttc ctgcaggcag cggcagaagc ccgccatcag ctcttcgggg gtcgcggcct
     2821 tccacgcctc cgcgagcagc cagcccgcga tggcattggc gtgcatgctc gcgccgcggc
     2881 cgagatgcgg cgcctcgtcc tcgccgtcgt agatcgcgct gacccggctc ggatagaacg
     2941 cgaggtattg ccggataggc gctgcgtcgt cgtacggctc ctcgtaggac cacgcggcgt
     3001 tctctgctac cgcgtcgtcg acccggagcg tccagtagct cgcgtccccc ttgaacgggc
     3061 aatgcgtgcg gtgctcggtc ctccctagca gctcgagccg cacgtcctcg cgcggaaagt
     3121 agtacatcgg cggcagccgc gtttcgtgca gcaccagcac ccgcgtcgaa tccgctaccg
     3181 aagtccggtt gaactcgacc cgcacgcgcc gcgggttttt ttcgaaacga atttcgtaac
     3241 cgggttgtcg ctttccttcc atatcaatcc taacgataaa gcattaccgc ctcgcgctcc
     3301 tcgcgccagg cctcttcgtc cagccgcgca gccgcatcga gatcggccgg cccggcggcg
     3361 ggatcgtcca cccagcggcg caacagctcc gagccagtga tgaggtcgat ggcgaggcgc
     3421 ccttgctcgt attcgtaggc gaagtcgcgc cacaggctgt agtccggctg cagcaggcgc
     3481 agcgccttca aggcgagcgc gaccagccgc cacggccgaa aagcgcggtg atcatacgcc
     3541 gcgtcctcga cgtggatctg cagccccgcg cacagctcgc cgacgtgttt ctggaacgtc
     3601 ggctcgaacc agcaggcgcg cagccggcag ccctgcatcc actgcggcgc cagggaatgc
     3661 attttttgca gcagccctct cggctcgaga tcgggcgcgc cgaacagctc gagcggccgc
     3721 gtcgtgccgc gcccttcgga tagcttggtg ccttccagca tcacggtccc gggatagcag
     3781 cgcgccattc cgggatttgg cgcattcggg cttggattca cccaggcgcg ctcgccgagc
     3841 ggccagcccc agcccggcgg cgcgtcaggc gcccagcctt gcatggcaac gacctggtag
     3901 tcgagatcga ggccgaggct gcggatgagc catttcccgg cctcgcccag cgtcaggccg
     3961 tgccgcatcg gcagcggccc tgcgccgacc aagctttccc agcccgggcg cagccgcaac
     4021 ccctcgaccg gacggcccgc gggattgggc cggtcgagca gccagacgat tttccgcttt
     4081 ctggcagatt cctccagcac gtagcgcagc gtcgtcagga aggtatacac gcggcagcct
     4141 acgtcctgga gatcgaccag cagaacgtcg aagctgtcca tcatctgcgc ggtaggccgg
     4201 cgcacgctgc cgtagaggct gaacaccggg atgccgtgca ccggatcggt gaagtccgac
     4261 gattcgatca tgttgtcctg cttgtcgccg cgcaggccgt gctgcggccc gaacgctgcc
     4321 gtcacatcga gatcgtccag tgctgcaagc gcgtccagag agtgaacgag agcggccgtc
     4381 accgaagcgg ggtgggcgag cagcgcga
//
LOCUS       DASPXW010000261         6375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1840905, whole genome shotgun sequence.
ACCESSION   DASPXW010000261 DASPXW010000000
VERSION     DASPXW010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6375
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1840905"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VEL04_06000"
     CDS             complement(<1..552)
                     /locus_tag="VEL04_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47954.1"
                     /translation="MRNNDHDVTGSVRTTDPTSVGAEVMRVFKGLFAGAAAPELKRAF
                     REAAGLYRGEHADYFACDTEYHDIQHVLDVTLAMARLMDGYQRGVHNGEEPLTKELFT
                     VGALAALFHDFGYLRRRHDHRHHYGAEYTLIHVSRGATFLRRYVSALGLGDEMARLAA
                     TLVHFTGYERPAETIRISGTLPRR"
     gene            619..840
                     /locus_tag="VEL04_06005"
     CDS             619..840
                     /locus_tag="VEL04_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HYT47955.1"
                     /translation="MTDFPLTEEEKAQIRERLQALEIEHHDLDDVIGRLSADVAQDQL
                     QLRRLKKRKLFLKDQIQRLRTRLIPDIVA"
     gene            837..1289
                     /gene="dtd"
                     /locus_tag="VEL04_06010"
     CDS             837..1289
                     /gene="dtd"
                     /locus_tag="VEL04_06010"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HYT47956.1"
                     /translation="MIALLQRVAEAAVAVDGAVVGAIGPGLVALVGVERGDGEAEAAR
                     LAARVIGYRIFADANGKMNLSLKDTQGGLLAVPQFTLAADTDSGARPSFSSAAPPQDG
                     EKFFDRFVEIAGTAVTVKTGRFGAHMKLSLVNDGPVTFWLQVSPQRTS"
     gene            1386..2441
                     /locus_tag="VEL04_06015"
     CDS             1386..2441
                     /locus_tag="VEL04_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HYT47957.1"
                     /translation="MFEQFIGTKPVEERHRIDVASLEAFLELKIGSLEQFKGGQSNPT
                     YRILAADGRRFVLRRKPPGKLLPSAHAVDREYRVIRALHPTGFPVARPHVLCEDESVI
                     GTAFYVMDHVEGRVLWDPALPGMTRAERARIWDEQNRVIALLHAIDYRAVGLEAFGKP
                     GNYIARQAERWSKQYRASETGKIEAMDHLIAWLPHNIPPEAGTSVVHGDYRLDNLIFH
                     PAEARILAVLDWELSTLGDPLADFAYHCMGWHIPPDKFRGMAGLDLEALGIPSEESYL
                     KDYCKRTGRAGVASSHWDFYLAYNLFRIAAICQGIAKRVLDGTAASSFAEEAASKTAP
                     LAELGWQQVEKILRRAA"
     gene            2438..3676
                     /locus_tag="VEL04_06020"
     CDS             2438..3676
                     /locus_tag="VEL04_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYT47958.1"
                     /translation="MNFEYTPKVEQLRKKLIAFMEQQVYPAEGAWQAHVKGERRWEPV
                     PVIEELKPKARAAGLWNLWRPKSHGGTLTNLEYAPLCEIMGRVLWAPEVFNCSAPDTG
                     NMETLLKYGTAEQVREWCEPLLEGSIRSAFLMTEPAVASSDATNIQTRIRREGDRYVV
                     NGVKWWSSGAGDSRCRIYIVMGKTDPDNASRHSQQSMVLVPADAPGIKLLRHLPVFGY
                     DDAPHGHMEVELKDVRVPVSNVLLGEGRGFEIAQGRLGPGRIHHCMRLIGQAERALEK
                     LCRRATERVAFGRPVAEQGVTRERIAESRIMIDAARYMVLNAAWKMDTVGNKGAKKEI
                     AMIKVFAPNVACQVIDWAMQVHGGGGVSDDFGLAYAYAHARTLRFADGPDEVHRNQIA
                     KMELGEQTSLGEALGKVRRM"
     gene            3673..4782
                     /gene="ald"
                     /locus_tag="VEL04_06025"
     CDS             3673..4782
                     /gene="ald"
                     /locus_tag="VEL04_06025"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HYT47959.1"
                     /translation="MIVGVPKETKTHEYRVGLTPTSVREVVAHGHEVLMEKGSGLGIG
                     AADSAYEKAGARIVEDVFAKAELIVKVKEPQRPERNQLRERHILFTYLHLAPDPGQAK
                     DLAACGAVCIAYETVTRQGGGLPLLAPMSEVAGRMSVQVGASCLEKARGGMGILLGGV
                     PGVPPAKITILGAGVAGSSAARLAVGSGARVYALDRSLEVLRRMESEFGARVVTVFSS
                     RDAVEQHVLSADLVIGSVLVAGAAAPKLVSRAMVTAMKPGSVVVDIAIDQGGCFETSR
                     PTTHADPTYVVDDVVHYCVTNMPGAVPRTSTYALNNATLPFVLALADKGWKRALAEDP
                     HLRNGLNVARGKVTHPEVAAALGEPYFPPEEMVLS"
     gene            4800..5450
                     /locus_tag="VEL04_06030"
     CDS             4800..5450
                     /locus_tag="VEL04_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HYT47960.1"
                     /translation="MLRLCGFHVSNYHNKVRLALLEKGVPFEEDANCRPSQSEDWLAR
                     SPIGKVPIIELDGGRRIAESEVICEYLEEAFPQKPLLPKDPFERAKVRELVTFIELHV
                     ELVARRLYGKVFFGRDVAEEVQQSAQKELAKGLRALKALAKFAPYIAGSELTLADCAA
                     FVSLPLVSLATKHAYGRDFLADLPQLKPYLKMLGERPAFAKVNEDRKAAQAAMIRK"
     gene            5447..6076
                     /locus_tag="VEL04_06035"
     CDS             5447..6076
                     /locus_tag="VEL04_06035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HYT47961.1"
                     /translation="MNPLQEYLRYVVTLTAVLDPFLAVPIFLSVTSPGDRKGRLRLAR
                     VVTITVFAVLAGAALFGEVLLRLIGASVSAFQVGGGLVLLLMALAMLNARVGEMRQTR
                     AEAAELEAGELSGVVPLAVPLLAGPGAIGTTIIAAQQGGVPHLAALVACIALVCVVLW
                     AMLRLAHTVAPRMGTTGLNIATRLFGLLLAAIAIQTMAEGLRQLFPGLA"
     gene            complement(6048..>6375)
                     /locus_tag="VEL04_06040"
     CDS             complement(6048..>6375)
                     /locus_tag="VEL04_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYT47962.1"
                     /translation="QSGRKDLEGYASPLADLRYLPFAAYVQGLAAMGDPLPDLAQIRC
                     PTLVLISSHSTMTDPARTRAIMQAIPGVEIVQVDAEHWIATEQPEAMCRAIEDWIARQ
                     GLERAA"
BASE COUNT         1065 a         2085 c         2183 g         1042 t
ORIGIN      
        1 gcggcgcggc agcgttccgg aaatgcggat ggtttcggcc gggcgctcgt agccggtgaa
       61 gtggacgagc gtcgcggcga ggcgcgccat ctcgtcgccc aggccgaggg cgctcacgta
      121 gcggcgcaga aaggtcgcgc cgcgcgagac gtggatcaac gtgtactcgg cgccgtagtg
      181 atgcctatgg tcgtggcgcc ggcgcagata gccgaaatcg tggaacagcg cggcgagcgc
      241 gccgacggtg aaaagctcct tcgtcagcgg ttcttcgccg ttgtgcacgc cgcgctggta
      301 gccgtccatc agccgcgcca tcgcgagcgt gacgtcgagc acgtgctgga tgtcgtggta
      361 ctcggtgtcg caggcgaaat aatcggcatg ctcgccgcgg tacagcccgg ccgcttcgcg
      421 gaacgcgcgc ttcagctcgg gcgccgccgc gcccgcgaac aggcccttga agacacgcat
      481 cacctcggcc ccaaccgagg tggggtcggt ggtgcgcaca gaaccggtga cgtcatggtc
      541 gttattgcgc atgcgggggg cttttcgctt gcccagtata aggttacgat gggcgcgagc
      601 gcgtgacttt tatcacaggt gacggacttc cctcttaccg aagaggaaaa agcgcaaatc
      661 cgcgagcgcc tgcaggctct ggaaatcgag caccacgacc tcgatgacgt gatcggcagg
      721 ctctccgccg acgtcgccca ggaccagctc cagctgcggc gcctgaagaa gcgcaagctg
      781 ttcctcaagg accagatcca gcgcctgcgc acgcgcctca tccccgacat cgtcgcgtga
      841 tcgctctgct gcagcgcgtg gccgaagcgg ccgtggccgt cgacggcgcg gtcgtcggcg
      901 cgatcgggcc gggactggtg gccctggtcg gcgtcgagcg cggcgacggc gaagccgagg
      961 ccgcgcgcct cgccgcgcgg gtcatcggct atcgcatctt cgccgacgcg aacggcaaga
     1021 tgaatctttc cctgaaggac acccaaggcg gtctgctcgc cgtcccccaa ttcacgctcg
     1081 ctgccgacac cgacagcggc gcgcggccga gcttctcgag cgctgctcca cctcaagacg
     1141 gcgagaagtt tttcgatcga ttcgtcgaga tcgcgggtac tgcggttaca gtcaaaaccg
     1201 gacgcttcgg cgcccacatg aagctcagcc tcgtgaacga cgggccggtg accttttggc
     1261 tccaggtttc tccgcaacgt acgtcctgaa agcggcgggc gagacgatct cgtactcgaa
     1321 cagcgcccag cccctgagca cgtacacgaa ctgcagcgcg cgaattctaa ggtaagctga
     1381 ccgggatgtt cgagcagttc atcggcacca agcccgtcga ggagcgccat cgcatcgacg
     1441 tggcttccct cgaggctttt ctcgagctga aaatcggcag tctcgagcag ttcaagggcg
     1501 ggcagtccaa tcccacctac cgcatcttgg cagccgacgg caggcgcttc gtgctgcggc
     1561 gcaagccgcc gggcaagctg ctgccctcgg cgcatgcggt cgatcgcgaa taccgcgtga
     1621 tccgcgcgct gcacccgacc ggttttccgg tcgcgcgccc tcacgtgctg tgcgaggacg
     1681 agagcgtgat cggcaccgcg ttctatgtca tggaccatgt cgaggggcgc gtgctgtggg
     1741 acccggcgct tcccgggatg acgcgcgccg agcgtgcccg catctgggac gagcagaacc
     1801 gcgtcattgc gctgctgcat gcgatcgatt accgcgcggt cgggctcgaa gccttcggca
     1861 agcccggcaa ttacatcgcg cggcaggccg agcgctggag caagcaatac cgcgcctcgg
     1921 agaccggaaa aatcgaggcc atggaccacc tcatcgcctg gctgccgcac aacattccgc
     1981 ccgaggcggg gacctcggtg gtgcatggcg actaccgcct cgacaacctg attttccatc
     2041 ccgcggaagc gcgcattctg gcggtgctcg actgggagct ctcgaccctc ggcgatccgc
     2101 tcgccgattt cgcctaccac tgcatgggct ggcacatccc gcccgacaag ttccgcggca
     2161 tggcggggct cgatctcgag gccttgggta ttccttccga agagagctat ctcaaggatt
     2221 attgcaagcg caccgggcgg gccggcgtcg cgtcgtcgca ctgggacttc tatctcgcct
     2281 acaacctgtt ccgcatcgcg gcgatctgcc aggggatcgc caagcgcgtg ctcgacggca
     2341 cggcggcaag ctcgttcgcc gaggaggccg cgagcaagac cgcgccgctc gccgagctcg
     2401 gctggcagca ggtggagaaa atactgagga gggcagcgtg aacttcgaat acacgccgaa
     2461 agtcgagcag ctgaggaaga agctcatcgc tttcatggag cagcaggtct atccggccga
     2521 gggcgcctgg caggcgcacg tgaagggcga gcgccgctgg gagccggtgc cggtgatcga
     2581 ggagctgaaa ccgaaggccc gcgccgcagg gctgtggaac ctgtggcggc cgaaatcgca
     2641 cggcggcacg ctgaccaacc tcgagtatgc gccgctgtgc gagatcatgg gccgcgtgct
     2701 ctgggcgccg gaagtgttca actgctccgc gcccgatacc ggcaacatgg agacgctcct
     2761 caagtacggc acggccgagc aggtgcgcga gtggtgcgag ccgctcctcg aaggcagcat
     2821 ccgctcggcg ttcctcatga ccgagccggc cgtcgcctcc tcggacgcca ccaacatcca
     2881 gacccgcatc cggcgcgagg gcgatcgcta cgtggtgaat ggcgtcaagt ggtggtcgag
     2941 cggcgccggc gattcgcgct gcaggatcta catcgtgatg ggcaagaccg atccggacaa
     3001 cgcctcgcgc cacagccagc agtcgatggt gctggtgccc gccgatgctc cgggcatcaa
     3061 gctgctgcgc cacctgccgg tattcggcta cgacgatgcg ccccatggcc acatggaggt
     3121 cgagctgaag gacgtgcgcg tgccggtgtc caacgtgctg ctcggcgagg ggcgcggctt
     3181 cgagatcgcg caggggcgcc tcgggcccgg gcgcatccac cactgcatgc gcctgatcgg
     3241 ccaggcggag cgcgcgctcg agaagctgtg ccggcgcgcg accgagcggg tcgcgttcgg
     3301 ccgtccggtt gccgagcagg gcgtgacgcg cgagcgcatc gccgagtcga ggatcatgat
     3361 cgacgccgca cgctacatgg tcctgaacgc cgcctggaag atggacacgg tcggcaacaa
     3421 gggcgcgaag aaggagatcg ccatgatcaa ggtgttcgcg cccaacgtcg cctgccaggt
     3481 gatcgactgg gcgatgcagg tgcacggcgg cggcggggtg tcggacgact tcggtctcgc
     3541 ctacgcctac gcgcatgcca ggaccttgcg cttcgccgac ggccccgacg aagtgcaccg
     3601 caaccagatc gccaagatgg agctcggcga gcagaccagc ctcggcgagg cgctcggcaa
     3661 ggtcaggcgc atgtgatcgt cggggtgccg aaggagacca agacccacga gtaccgcgtc
     3721 gggcttaccc cgacctcggt gcgcgaggtc gtggcgcacg gccacgaagt cttgatggaa
     3781 aaaggttccg gcctgggaat cggcgccgcg gattcggctt acgaaaaggc gggtgcccgg
     3841 atcgtcgaag acgtgtttgc gaaagccgag ctgatcgtca aggtgaagga gccgcagcgc
     3901 ccggagcgca accagctcag ggagcggcac atcctcttca cctacctgca tctcgcgccg
     3961 gatcccgggc aggcgaagga cctcgccgct tgcggggcgg tctgcatcgc ctacgagacc
     4021 gtgacgcgcc aggggggcgg cctgccgctg ctcgcgccga tgtcggaggt cgcgggcagg
     4081 atgtcggtgc aggtcggcgc cagctgcctg gaaaaggccc gcggcggcat gggcatcctg
     4141 ctcggcggcg tgccgggcgt gccgcccgcc aagatcacca tcctcggcgc cggcgtcgcc
     4201 ggctcgagcg cggcgcgtct cgcagtaggc tcgggtgcgc gggtgtatgc cctggatcgt
     4261 tctttggaag ttctaagacg aatggaaagc gaattcggcg cgcgggtcgt gaccgtattc
     4321 tcgagccggg acgcagtcga gcagcatgtg ctctcggccg atctggtgat cggctcggtg
     4381 ctcgttgcgg gggcggccgc gcccaagctc gtctcgcgcg cgatggtgac ggcgatgaag
     4441 ccggggtcgg tagtggttga tatcgccatc gaccagggcg gctgcttcga gacctcgcgc
     4501 cccaccacgc acgccgatcc gacctacgtg gtggacgacg tcgtgcatta ctgcgtcacc
     4561 aacatgcccg gcgccgtgcc tcgtacttcg acctatgcat tgaacaacgc cacgctgccc
     4621 ttcgtgctcg cgcttgccga caagggctgg aagcgcgcgc tcgccgagga tccccacctc
     4681 aggaacggcc tcaatgtcgc gcgcggcaag gtcacccatc ctgaggtggc cgcggcgctc
     4741 ggcgagccgt attttccccc ggaagaaatg gttctaagct agctgtttcg gagaaacaca
     4801 tgctcagact gtgcggcttc cacgtcagca actatcacaa caaggtgcgc ctcgcgctgc
     4861 tcgagaaggg cgtgcccttc gaagaagacg cgaactgcag gccgtcgcag agcgaggact
     4921 ggctggcgcg ctcgccgatc ggcaaggtgc cgatcatcga gctcgacggc ggccggcgca
     4981 tcgccgagtc ggaagtgatc tgcgagtacc tcgaagaggc gttcccgcaa aagccgctgc
     5041 tgccgaaaga ccctttcgag cgtgccaagg tgcgcgagct ggtgacgttc atcgagttgc
     5101 acgtcgagct ggtggcgcgc cggctgtacg gcaaggtgtt tttcggcagg gacgttgccg
     5161 aggaagtgca gcaatcggcg cagaaggagc tcgccaaagg gctgcgtgcg ctcaaggcgc
     5221 tggcgaaatt cgcgccttac atcgctggaa gcgagctgac cctcgccgat tgcgcggcgt
     5281 tcgtcagcct gccgctggtc tcgctcgcca ccaagcatgc gtacggccgc gattttctcg
     5341 ccgacctgcc gcagctcaag ccctacctga agatgctcgg cgagcggcct gcattcgcca
     5401 aggtcaatga ggaccgcaag gccgcgcaag ctgcaatgat caggaagtga acccgctgca
     5461 ggagtacctg cgctacgtcg tcacgctgac cgcggtgctg gacccgtttc tcgccgtgcc
     5521 gatctttctc agcgtcactt cgcccggcga ccgcaaggga cgcctgcgcc ttgcgcgcgt
     5581 cgtcaccatc acggtgttcg ccgtgctcgc cggcgcggcg cttttcggcg aggtcctgct
     5641 gcgcctgatc ggcgcgagtg tttcggcatt ccaggtgggc ggcgggctgg tgctgctgct
     5701 gatggcgctc gcgatgctga atgcgcgggt tggagagatg cggcagacgc gcgccgaggc
     5761 cgcggagctc gaagcgggcg agctctcggg cgtggtgccg ctcgccgtac cgctgctcgc
     5821 cgggccgggc gcgatcggca ccaccatcat cgcggcccag cagggcggcg tgcctcatct
     5881 cgccgcgctc gtcgcctgca tcgcgctggt gtgcgtcgtc ctgtgggcca tgctgcgcct
     5941 cgctcacacg gtggctccac ggatggggac gaccggcctc aacatcgcca cgcgcctgtt
     6001 cggcctgttg ctcgcggcca tcgccatcca gaccatggcc gaaggactca ggcagctctt
     6061 tccaggcctt gcctagcgat ccagtcctcg atcgcgcggc acatggcctc gggctgctcg
     6121 gtcgcgatcc agtgctcggc gtcgacctgc acgatttcca cgcccgggat cgcctgcatg
     6181 atggcgcggg tacgagccgg gtcggtcatg gtgctgtggc tcgatatcag gaccagtgtc
     6241 gggcagcgga tctgcgccag atcgggaagc gggtcgccca tcgcggcgag gccctgcacg
     6301 taggcggcga acggcaagta gcgcaggtcg gcgagcggcg aggcatagcc ctcgagatcc
     6361 ttcctgccgc tctgc
//
LOCUS       DASPXW010000262         2397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1087335, whole genome shotgun sequence.
ACCESSION   DASPXW010000262 DASPXW010000000
VERSION     DASPXW010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087335"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..116
                     /locus_tag="VEL04_06045"
     CDS             <1..116
                     /locus_tag="VEL04_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004550955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HYT47963.1"
                     /translation="SFPSNLTAMMFGFKEKPQFTVENEKEIARPPKVDFTK"
     gene            100..963
                     /locus_tag="VEL04_06050"
     CDS             100..963
                     /locus_tag="VEL04_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011881321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HYT47964.1"
                     /translation="MISLSSLFLVAVAAAQIAVPPLKARVTDLTGTLSAQQRSALEQT
                     LAEFERRKGAQVAVLMVPSTQPETVEQYAVRVEESWKLGRKGVEDSVLLVVAKNDRKL
                     HIEVGYGLEGVLPDAIAKRIIEDDITPRFKQGDFYSGVRAGTDRIMRVIEGEKLPPPR
                     ARAEARGASPDVSQWLFITFFIAVVAGPIFHRLFGRVLGPGILGGGIGAFAWFITGFA
                     LIAALTGVIAFVITFLAGLGRASGWSGSGGGWSSGGGFSSGGGGFSSDSGGGFSGGGG
                     SSGGGGASGSW"
     gene            963..1454
                     /locus_tag="VEL04_06055"
     CDS             963..1454
                     /locus_tag="VEL04_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HYT47965.1"
                     /translation="MHPSRLLKHLLVPHWLALRQFPKSSLRKIEEAIKASEHQHDGEL
                     RFAAEAGLPFHFLKFSPRRRAEALFSELRVWDTQHNSGVLIYVQLLDRRIEIVADRGI
                     AAKVEQAEWETVCRRMEESFKKKQYLDGALEGIERITQILARHFPPRARHPNELPDKP
                     VVL"
     gene            1454..2143
                     /locus_tag="VEL04_06060"
     CDS             1454..2143
                     /locus_tag="VEL04_06060"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HYT47966.1"
                     /translation="MKYELYYWPSIQGRGEFIRLALEDAGADYVDVARRPKGMRAMMH
                     AMESASVKRAPFAPPFLRAGELLVAQTANILFFLGPRLGLAPKSEAGRLWLHQLQLTI
                     ADWLVEAHDTHHPIGGGLYYEDQKEESLRCAEHFRTERLPKFLGYFERISPELKAHNY
                     VNLSLFQMIEGLRYAFPLAMKSLEKKHSRLVALHDRVAERPRIAAYLSSKRRISFNQQ
                     GIFRRYPELDG"
     gene            2143..>2397
                     /locus_tag="VEL04_06065"
     CDS             2143..>2397
                     /locus_tag="VEL04_06065"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYT47967.1"
                     /translation="MAQQSFAALRQPVFRVYFFGAASAMMADSIEHVISYWIMFEKFQ
                     SPALAGFAVISHWAPFLLFSVWSGAAADRFDPRRIIQAGMV"
BASE COUNT          398 a          790 c          789 g          420 t
ORIGIN      
        1 gctcgttccc cagcaacctc acggcgatga tgttcggctt caaggaaaag ccgcagttca
       61 cggtggagaa cgagaaagaa atcgcgcgac cgccgaaggt tgatttcact aagtagtctc
      121 ttccttgttg cagtcgcggc ggcgcagatc gctgttccgc cgctgaaggc gcgcgttacc
      181 gacctcaccg gcacgctcag cgcgcagcag cgctcggcgc tcgagcagac gcttgccgag
      241 ttcgagcgcc gcaagggcgc gcaggtcgcg gtgctgatgg tgcccagcac acagcccgag
      301 accgtcgagc agtacgcggt gcgcgtggaa gagtcgtgga agctcggccg caagggcgtc
      361 gaagacagcg tgctgctcgt cgtcgccaag aacgaccgca agctgcacat cgaggtgggc
      421 tacgggctgg agggcgtgct gcccgacgcc atcgccaagc gcatcatcga ggacgacatc
      481 acgccgcgct tcaagcaagg cgacttctac agcggcgtcc gcgccggcac cgaccgcatc
      541 atgcgcgtga tcgaaggcga gaaactgccg ccgccgcgcg cgcgcgccga ggcgcgcggc
      601 gcctcgcccg acgtgagcca gtggctcttc atcacgttct ttatcgccgt ggtcgccggc
      661 cctatcttcc atcgcctttt cgggcgggtg ctcggccccg gcatcctggg cggcgggatc
      721 ggcgcctttg cctggttcat caccggcttc gcgctcatcg cagcgctgac gggcgtgatc
      781 gctttcgtca tcactttcct cgcgggcctc ggccgcgcga gcggctggag cggctccggc
      841 ggcggctggt catcgggtgg cggcttcagc agcggcggcg ggggtttcag cagcgatagc
      901 ggcggcggct tcagcggcgg cggcggcagc tcgggcggcg gcggcgcgag cgggagctgg
      961 tgatgcaccc cagccgtctg ctgaagcacc tcctcgttcc gcactggctc gccctgcggc
     1021 agtttccgaa atcctcgttg aggaaaatcg aggaggcgat caaggcctcg gagcaccagc
     1081 acgacggcga gctgcggttt gccgccgagg ccggtctgcc ttttcatttc ctgaagttca
     1141 gcccgcgtcg aagagccgag gcgctgttct ccgagctgcg cgtctgggac acgcagcaca
     1201 acagcggcgt gctgatctac gtgcagctcc tcgaccgcag gatcgagatc gtcgccgacc
     1261 gcggcatcgc cgcgaaggtt gagcaggccg agtgggaaac cgtctgccgc cggatggagg
     1321 aatcattcaa aaagaaacaa tatctggacg gcgcactcga gggcatcgag cgcatcacgc
     1381 agatccttgc gaggcatttc ccgccgcgcg cgcgccatcc gaacgagctg ccggacaaac
     1441 cggtggtgct gtaatgaaat acgagctcta ttactggcct tcgatccagg ggcgcggcga
     1501 gttcatccgc ctcgcgctcg aggacgcggg cgccgattac gtcgacgtcg cgcgccggcc
     1561 gaagggcatg cgggcgatga tgcacgcgat ggaaagcgcc tccgtgaagc gcgcgccgtt
     1621 cgcgccgccc ttcctgcgcg caggcgaatt gctggtcgcg cagacggcca acatcctctt
     1681 ctttctcggg ccgcggctcg gcctggcgcc gaaaagcgaa gccggccgcc tgtggctgca
     1741 ccagctgcag ctcaccatcg ccgattggct ggtcgaggcg cacgacacgc atcatccgat
     1801 cggcggcggt ctctactacg aagatcagaa ggaggagtcg ctgcgctgcg ccgaacattt
     1861 ccgcaccgag cgcctgccga agttcctggg ctattttgaa agaatcagtc ctgagctgaa
     1921 ggcgcacaac tacgtcaatc tgtctctgtt ccagatgatc gaggggctgc gctacgcatt
     1981 cccgctcgcc atgaagtcct tggaaaagaa gcattcaagg ctggtggcgt tgcacgaccg
     2041 cgtggcggag cgtccgcgca tcgcggccta cctctcctcg aagcgccgca tttcgttcaa
     2101 ccagcagggt atcttccgcc gctatcccga gctcgacggc tgatggcgca acagtccttc
     2161 gccgccctgc gccagccggt gttccgcgtc tatttcttcg gcgcggcctc ggcgatgatg
     2221 gccgacagca tcgagcacgt catcagctac tggatcatgt tcgagaaatt ccagtcgccg
     2281 gcgctcgcgg gcttcgcggt gatctcgcac tgggctccgt tcctgctgtt ctcggtgtgg
     2341 tcgggcgccg ccgcggaccg tttcgacccg cgccgcatca tccaggccgg catggtg
//
LOCUS       DASPXW010000263         2292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_79249, whole genome shotgun sequence.
ACCESSION   DASPXW010000263 DASPXW010000000
VERSION     DASPXW010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79249"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..1758
                     /locus_tag="VEL04_06070"
     CDS             70..1758
                     /locus_tag="VEL04_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HYT47968.1"
                     /translation="MKSDLKTRHGGKILADALVAQGASMAFGVPGESYLPVLDGLHDV
                     RGRFSFIVCRQEGGASYMAEAYGKLTGEPGILFVTRGPGASNGAIGVHTALQDSTPMV
                     VFIGQVPNEFAEREAFQEIDYRRMYGQMAKWVGQIDRVERIPEYVSRAFHTACAGRPG
                     PVVLALPEDTLFSEAAVADAPRHHTVRPAPSPESIAELKVLLEKARRPFVILGGGGWE
                     RDSCTRLQRWIEANGLAAGTSFRCQDLFDNRSASYAGDVGIGINPKLAQRVKEADVLL
                     VIGARLGEMTTGGYTLVQAPLPQQALVHVHAGAEELGRVYRPALAINSGMAQFVKALE
                     RLSFKSSSWNESTAAARNEYLEWTAPRPMPGKVQYGEVIQWLSNNLPEDTIVAGGAGN
                     FAGWLHRHFRYKGFRTQLGSTNGSMGYGYPAAVAAKLAQPQRPVIALCGDGDFLMTGQ
                     EIATAAQYGAAFVALVVNNGLYGTIRMHQEREYPGRVFGTELKNPDFAAYARAFGGHG
                     EMVERTEDFAAAFERASRSGKPAIIELRIDPDAITPSTTLSAIRDKALKSRAGR"
     gene            1786..>2292
                     /locus_tag="VEL04_06075"
     CDS             1786..>2292
                     /locus_tag="VEL04_06075"
                     /inference="COORDINATES: protein motif:HMM:NF024671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panacea domain-containing protein"
                     /protein_id="HYT47969.1"
                     /translation="MAVPAIQFQPEQALELVLYVAKRLPHPGFHSISKVLYFADREHL
                     SRYGSLMTGDTYYAMRHGPVPSAIYNLLKAAAGRQEPAIPLGWFELVAGVLAVEGEHR
                     IRPLRDPNLEMISAAQRACLDASIKANGRLSFSRLAKKSHDAAWKSADQNDIIELAAI
                     AKTLPNAKE"
BASE COUNT          423 a          746 c          746 g          377 t
ORIGIN      
        1 atgcgccgca gcgtgttgat gtcgtccatc gaccgaatac tataatcgaa gccttcctcc
       61 ggaccagaca tgaaatccga tctgaagacg cgccacggcg gcaagatcct cgcggatgcg
      121 ctcgtcgctc agggcgcaag catggcgttc ggcgttcccg gcgagagcta cctgccggtg
      181 ctcgacggcc tgcacgacgt gcgcgggcgg ttctcgttca tcgtctgcag gcaggagggc
      241 ggcgcctcct acatggcgga agcctacggc aagctcaccg gcgagccggg gatcctgttc
      301 gtcacgcgcg gtccgggcgc gagcaacggc gccatcgggg tgcacaccgc gctgcaggat
      361 tcgacgccga tggtggtgtt catcggccag gtgccgaacg agttcgccga gcgcgaggcg
      421 ttccaggaga tcgattaccg ccgcatgtac ggccagatgg cgaaatgggt ggggcagatc
      481 gaccgcgtcg agcgcattcc cgagtacgta agccgtgcgt tccacaccgc gtgcgccggc
      541 cggccggggc cggtggtgct cgcgttgccc gaggacacgc tgttttccga agccgcggtc
      601 gccgatgcgc cgcgccatca caccgtgcgg ccggcgccgt ccccggaaag cattgcagag
      661 ctgaaagtgc tgctcgaaaa agcgcgacgg ccgttcgtaa tcctcggcgg cggcggctgg
      721 gagcgcgaca gctgcacgcg cctgcagcgc tggatcgagg cgaacgggct tgcggcaggt
      781 acctcgttcc gctgccagga cctgttcgac aaccgcagcg cgagctacgc cggcgacgtc
      841 ggcatcggca tcaacccgaa gctcgcgcag cgcgtgaagg aagcggacgt gctgctggtg
      901 atcggagcgc gcctgggcga gatgacgacg ggcggctaca ccctggtgca agcgccgctg
      961 ccgcagcagg ccctggtcca tgttcatgcc ggcgccgagg agctcggccg cgtctaccgg
     1021 cctgcgctcg ccatcaactc ggggatggcg cagttcgtca aggcgctgga aaggctgagc
     1081 ttcaagagct cttcttggaa cgaaagcacc gcggccgccc gcaacgaata cctcgaatgg
     1141 accgcgccgc gcccgatgcc ggggaaagtg cagtacggcg aggtgatcca gtggttgagt
     1201 aacaacctgc ccgaagacac catcgttgcc ggcggcgcgg gcaatttcgc cggctggctg
     1261 caccggcatt tccgctacaa gggcttccgc acccagctcg gctcgaccaa cggctcgatg
     1321 ggctacggct accccgcggc ggtggcagcg aagctcgccc agccgcagcg tccggtgatc
     1381 gcgctatgcg gcgacggcga cttcctgatg accggccagg agatcgcgac cgccgcgcag
     1441 tacggcgccg ctttcgtcgc gctggtggtc aataacggcc tctacggcac gatccgcatg
     1501 caccaggagc gcgaatatcc cggccgggtg tttggcaccg agctcaagaa tccggatttc
     1561 gccgcctatg cgcgcgcctt cggcgggcac ggcgagatgg tcgagcgcac cgaggatttc
     1621 gccgccgcct tcgagcgcgc ctcgcgctcc ggcaagcctg cgatcatcga gctgcgcatc
     1681 gatcccgacg ccatcacgcc ttctacgacg ttgtctgcca tacgcgacaa agctttgaaa
     1741 tcgcgcgcgg gccggtaacg attcaaaggc tataatcggc gccacatggc tgtccccgcc
     1801 attcagttcc aacccgagca agccttggag ttggtgctat acgtggcaaa gcggttaccc
     1861 caccctggat tccactcgat ctccaaggtg ctgtatttcg ccgacagaga acatctgtcc
     1921 cgctacggca gcttgatgac cggtgacacc tattacgcga tgcgccatgg ccccgtgccg
     1981 agtgcgatct acaacctgct caaggctgct gccggaagac aggaaccggc aatccctctt
     2041 ggttggttcg agctggtcgc gggcgtactg gccgttgagg gagaacatcg tatccgtccc
     2101 cttcgggatc cgaacctcga aatgatttca gccgcacagc gggcgtgcct cgacgcgtcg
     2161 ataaaggcga acggccgact gagcttcagc cgattggcaa aaaagagcca cgatgcggcc
     2221 tggaaatcgg ccgatcagaa cgacatcatt gagttagccg cgattgcgaa gaccctgccg
     2281 aacgcaaagg aa
//
LOCUS       DASPXW010000264         2942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1588294, whole genome shotgun sequence.
ACCESSION   DASPXW010000264 DASPXW010000000
VERSION     DASPXW010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1588294"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VEL04_06080"
     CDS             complement(<1..400)
                     /locus_tag="VEL04_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HYT47970.1"
                     /translation="MAARRPRRFRLLVIGWKGLCYALGALVIAFALTQLWFFSHILYW
                     THFEPGSTAFMAARLEALRAKDPGAKLRREWVPYQRISVHLKRAVVAAEDAKFLDHEG
                     FDWEAIEKAILKNEQQGRVVAGASTISQQLA"
     gene            complement(409..1266)
                     /locus_tag="VEL04_06085"
     CDS             complement(409..1266)
                     /locus_tag="VEL04_06085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HYT47971.1"
                     /translation="MFGSNDRVLGAALGLSILLHAALLAIHFRTDALRWKPANQPLEV
                     VLVNAKTRDRPARSDVLAQANLDRGGNVDERRRARTPLPVTEPRSPGKDLAAAQRRRR
                     ELEAQQQRLLAQTRESATHVPKETVRQAPTDEPAAQPNGRDLADLALAAMRLQAQIDK
                     RIDEYQKRPRKQFIGANASEYRFAQYEEDWRIKVERIGSLNYPPGARGKLYGNLRLTV
                     TIRPDGSVETIELDRSSGLEVLDAAAFKIVRMATPFAAFPANIRRDTDLLVITRTWFF
                     GQGDKIWTE"
     gene            complement(1316..>2942)
                     /locus_tag="VEL04_06090"
     CDS             complement(1316..>2942)
                     /locus_tag="VEL04_06090"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HYT47972.1"
                     /translation="EYVGREPSAVEAAGVLLKLHAAPIFFYRRGKGRFQAAPEQTLKL
                     ALAGVEKKKRVHQTIAAWAAALARFDCPREIAALKDELLYAPDGNKPEAKALEQACRE
                     TGLAPARLFERCGLLADTRDYHLKRFLHEFFPHGSSFPPHEMPLPPADLPLASVRAFS
                     LDDVGTTEIDDAFSVQRTASGELRVGIHIAAPALGIAQGSPLDAIARGRLSTAYLPGC
                     KFTMLPEDVIERFSLDHGGEQPALSLYFDVSEHDASVRGTYSRIERVPIAANLRHAEY
                     DVLNEAFESGAKVGLPFEDELRTLWRIAGALEARRDRPGAGAMTLDYSFYVENGRVRI
                     VPRKRGAPLDKLVAEMMIFVNSTWGELLAEHDVAAIYRVQSTGKVRLSVHPEPHEGLG
                     VSCYAWMSSPLRRYVDLLNQWQLVAALRGQRAPFARNSDALLGALRAFEVTYARYDEH
                     QRAMETYWTLRWLQQEAIQSAEATVLRENLVRFDHLPLVMRVSSLPALDPGTRVRLDL
                     GQVDFLERTLSAVYRETLGRGVAVVDDDPAPTS"
BASE COUNT          478 a          971 c          980 g          513 t
ORIGIN      
        1 tcgccagctg ctggctgatg gtcgatgccc ccgccaccac cctgccctgc tgctcgttct
       61 tcaagatcgc cttctcgatc gcttcccagt cgaagccttc atggtcgagg aacttggcgt
      121 cctcggcggc caccaccgcg cgcttcagat gcaccgaaat gcgctgataa ggcacccatt
      181 cgcggcgcag cttcgcgccg ggatccttgg cgcgcagtgc ttcgagccgc gccgccatga
      241 acgcggtcga gccgggctcg aagtgcgtcc agtacaggat gtgcgagaaa aaccacagct
      301 gcgtcaaggc gaatgcaatg accagcgcgc cgagcgcgta gcacaagccc ttccacccga
      361 tgaccagcag ccgaaagcgc cgaggtcgtc tagcggccat cgagcctgct attcagtcca
      421 gatcttgtcg ccctggccga agaaccaagt gcgggtgatg accagcaggt ccgtatcgcg
      481 gcggatgttc gcagggaagg ccgcaaatgg cgtcgccatg cgcacgatct tgaatgccgc
      541 ggcgtcgagc acttcaagcc ccgaggagcg gtcgagctcg atcgtttcca ccgagccgtc
      601 gggccggatg gttaccgtga ggcgcaggtt gccgtagagc ttgccgcgcg cccctggggg
      661 gtaattcagc gacccgatgc gctcgacctt gattcgccag tcctcctcgt attgcgcgaa
      721 acggtattcg ctggcgttag cgccgatgaa ctgcttgcgc gggcgcttct ggtactcgtc
      781 gatgcgcttg tcgatctgcg cctgcaggcg catggcggcc agcgccaggt cggcgaggtc
      841 gcgcccgttc ggctgggccg cgggctcatc ggtgggcgcc tggcgcacgg tttctttcgg
      901 cacgtgggtg gcggattcgc gtgtctgcgc gagcaggcgc tgctgttgcg cctcgagctc
      961 gcgcctgcgc cgctgcgcgg ccgcaaggtc cttgccgggg ctgcgcggct cggtcacggg
     1021 cagtggcgtg cgtgcccgcc gccgctcgtc gacgttgccg ccacggtcga ggtttgcctg
     1081 cgcgagcacg tccgagcgcg ccggccgatc acgcgtcttc gcattgacga gcactacttc
     1141 gaggggctgg tttgccggct tccagcgcag cgcgtcggtc cggaagtgaa tcgcgagcag
     1201 cgcagcgtga agcaggatcg agaggcctag cgccgcaccg agcacccggt cgttgcttcc
     1261 gaacattaag tacattctca cgtcaacctg acaagccttt catattttag gctttttagc
     1321 tagtgggtgc cgggtcatcg tccactaccg cgacgccacg gccgagcgtc tcacgataga
     1381 ccgcggataa cgtgcgttca aggaagtcca cctggcctag gtctaggcgc acgcgcgtgc
     1441 ccggatcgag cgcgggcaac gacgaaacgc gcatgaccag cggcaagtgg tcgaagcgta
     1501 cgaggttttc gcgcagcacc gtagcttccg ccgactggat cgcttcctgc tgcagccagc
     1561 gcaacgtcca ataggtttcc atcgcccgct gatgctcgtc gtagcgcgcg taggtcacct
     1621 cgaaggcgcg cagcgcgccg aggagcgcgt ccgaatttcg cgcgaacggc gctcgctggc
     1681 cgcggagcgc ggctaccagc tgccactggt tgaggaggtc gacgtagcgc cgcagcggcg
     1741 agctcatcca ggcataacag gacacgccaa gtccttcgtg cggctcggga tgcacgctaa
     1801 ggcgcacctt gcccgtcgat tgcacgcggt agatcgccgc gacgtcgtgc tcggcgagca
     1861 actcgcccca ggtcgaattg acgaagatca tcatctcggc caccagcttg tcgagcggcg
     1921 cgccgcgttt tcgcggcacg atgcgcacgc gtccgttctc gacatagaag ctgtagtcga
     1981 gcgtcatcgc gccggcaccc ggacggtcgc gccgcgcctc gagcgcgccc gcgatgcgcc
     2041 acagggtgcg cagctcgtcc tcgaacggca gacccacttt cgcgcctgat tcgaacgcct
     2101 cgttgagcac gtcgtactcg gcatggcgca ggttcgccgc gatcggcacg cgctcgatgc
     2161 ggctataagt gccgcgcacg gacgcgtcgt gttcggaaac atcgaagtag agcgacaggg
     2221 ccggctgctc gccgccgtgg tcgagggaaa atcgctcgat cacgtcctcg ggcaacatgg
     2281 tgaacttgca gccgggcagg tacgccgtcg acagccgccc gcgcgcgatc gcgtcgagcg
     2341 gcgagccctg ggcaattccg agcgccgggg cggcaatgtg gatgccgacg cgcagctcgc
     2401 cggacgcggt gcgctgcact gaaaaagcat cgtcgatctc ggtagtgccg acgtcatcga
     2461 ggctgaacgc ccgcacgctc gccagcggca aatccgccgg cggcaacggc atttcgtgcg
     2521 gcgggaagct gctgccatgc gggaagaatt cgtgcaggaa gcgcttgagg tggtagtccc
     2581 gtgtatccgc aagcagcccg cagcgctcga acaggcgggc cggtgcaagc ccggtctcgc
     2641 ggcaggcttg ctccaatgcc ttggcttcgg gcttgttgcc gtccggggca tacagcagtt
     2701 cgtccttgag cgcggcgatc tcgcgaggac agtcgaagcg tgcgagcgcc gccgcccagg
     2761 ccgcgatcgt ttggtgcacg cgtttcttct tttcgacacc ggcgagcgcg agcttcagtg
     2821 tctgttcggg cgcggcctgg aaacgccctt tgccgcggcg gtagaagaag atcggtgcgg
     2881 catgcagctt gagcagcacg cccgccgcct caaccgcgct cggctcgcgc ccgacgtact
     2941 cg
//
LOCUS       DASPXW010000265         3626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1213973, whole genome shotgun sequence.
ACCESSION   DASPXW010000265 DASPXW010000000
VERSION     DASPXW010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213973"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1039)
                     /locus_tag="VEL04_06095"
     CDS             complement(<1..1039)
                     /locus_tag="VEL04_06095"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HYT47973.1"
                     /translation="MAEQAAVMNPLARLRARLAARPDTEHEQGILRLVISSMVFLYLL
                     PDALLKHDTLPLYVMGIHLVLALIIFLRIAYSTNISPARRVFAQLADVAAISWYMAVF
                     GESSAPLFLLYIWVTLGSGFRFGARYLVSELAMSVLGFGIVIYTNDFWREHMSLGIGL
                     LIGMFAVSLYVLSLVRRMFDALARAEAANLAKRRFISMVSHELRTPLNAIVGMADLLR
                     DTSLSREQADMLQTLRSSSNVMLGLVEDVLDFSKIEAGKVVLEKTDFDLHALVNSTCR
                     IVAAQAAAKGLEFAVSIMPEVPPGVRGDPHHLRQVLINLAGNAVKFTERGSVTVHVSA
                     QTESEAGVRLKF"
     gene            complement(1039..2136)
                     /locus_tag="VEL04_06100"
     CDS             complement(1039..2136)
                     /locus_tag="VEL04_06100"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HYT47974.1"
                     /translation="MARAEVRVLPSARNTVMVVDDQSTGRAILEQVVRSLDERVAVQG
                     FARPVDAVVWATRHASDLVLVDYMMPDMDGIELVKRLRALPGYEHVPIVMVTVHDDRK
                     VRYAALDAGITDFLTKPVDARECLARCRNLLTLRRQQLALEDRRRLLEHMVEDATREV
                     REREKETLLRLARAGEFRDEATGNHLIRMSRYSRLIANALGLEHDEAETVELAAPLHD
                     IGKIGIPDQILLKPAKLDDAEWQVMQRHPAIGHEILKGSASKYVRMGALIALAHHEKY
                     DGSGYPSGLVGDHIPLCARIVAVADVYDALTSVRPYKTAWPSERAFEYVASQAGRHFD
                     ARFAEAFAGMKKEVLQIQNEWRDPAVVGTKP"
     gene            2397..3371
                     /locus_tag="VEL04_06105"
     CDS             2397..3371
                     /locus_tag="VEL04_06105"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HYT47975.1"
                     /translation="MRKLFIPVVLAAALSAGSAGAQIVTKLSGIQPLEHPSSYAEKFF
                     SEQVSILTNGAIKVENYHNTQLGDAVANVQSVRNGTIGFTVVSASNLNQVVPAMDMLS
                     LPFIFKNEAHFWWYLAQPEAESFVKALEAKGIKKIAYIDSGSRNLFTQKAVRTPADLK
                     GQKIRVMASPVMVKTMEALGATGVPVAWAELYTALQTGVVDGAENNHPSVVAKKFYEV
                     SKFYTLDEHMRIPDLLVVSMKFYDSLNTQQKAAVMQAGALTQAYMRGAWKISENKDLA
                     ELKSKFTQIIEPNKQPFMDAVKPLVLAEAKRLEVVKTVSFILESGKKF"
     gene            3375..>3626
                     /locus_tag="VEL04_06110"
     CDS             3375..>3626
                     /locus_tag="VEL04_06110"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HYT47976.1"
                     /translation="MLKLLDRAVARTLTVVRAVCIAQAAALFLVVVLAVIGRYVFGRS
                     LTWTEEVPRYLLIWISFLGAAACVARREHVGFDVLFNALP"
BASE COUNT          638 a         1172 c         1194 g          622 t
ORIGIN      
        1 agaacttgag cctaacgccg gcctcggatt ccgtctgcgc cgagacatgc accgtgacgc
       61 tgccgcgctc ggtgaacttc acggcgttgc ccgcgaggtt gataagcacc tggcgcaagt
      121 gatgcgggtc gccgcgcacc cccggcggca cctccggcat gatcgagacc gcgaattcga
      181 ggcccttcgc cgccgcctgc gcggcgacga tgcggcaggt gctgttgacg agcgcgtgca
      241 ggtcgaagtc ggtcttctcc agcactacct tgccggcctc gatcttggag aagtcgagca
      301 cgtcctcgac caggccgagc atgacgttcg aggagctgcg cagcgtctgc agcatgtcgg
      361 cctgctcgcg gctgagcgag gtgtcgcgca gcaggtccgc catgccgacg atggcgttca
      421 ggggcgtgcg cagctcgtgg ctgaccatcg agatgaagcg gcgcttcgcg aggttcgccg
      481 cctccgcccg ggcgagcgcg tcgaacatgc gcctcaccag cgacaggacg tacaggctga
      541 ccgcgaacat gccgatcagc agcccgatcc cgagcgacat atgctcgcgc cagaagtcgt
      601 tggtgtagat gacgatgccg aaccccagca cgctcatggc gagctcggag accaggtagc
      661 gggcgccgaa gcggaaaccg ctgcccagcg tcacccagat atagagcagg aacagcggcg
      721 ccgaggactc gccgaacacc gccatgtacc agctgatcgc ggcaacgtct gcaagctggg
      781 cgaacacgcg ccgcgccgga gagatgttcg tcgaataggc gatgcgcagg aaaatgatca
      841 gcgcgagcac caggtggatg cccatcacgt acaagggcag ggtgtcgtgc ttcaggagcg
      901 cgtcgggcag caggtacagg aacaccatcg agctgataac cagccgcagg atgccctgct
      961 cgtgctcggt gtcgggccgc gcggcgagcc gcgcgcgcag ccgcgcgagc gggttcatga
     1021 cggcagcttg ctcggccatc agggcttcgt ccccacgacc gccgggtcgc gccactcgtt
     1081 ctggatctgc agcacttcct tcttcatgcc ggcaaaggcc tcggcgaagc gcgcatcgaa
     1141 atgccggccc gcctgcgagg cgacgtattc gaacgcacgt tcgctcggcc atgcggtctt
     1201 gtagggccgc accgaagtga gcgcgtcgta gacgtcggcg acggcgacga tgcgcgcgca
     1261 cagcgggatg tggtcgccca ccagcccgct gggatagccc gagccgtcgt atttctcgtg
     1321 gtgcgcgagc gcgatcagcg cgcccatgcg cacgtacttc gaggcgctgc ccttcaggat
     1381 ctcgtgcccg atcgccgggt ggcgctgcat cacctgccat tcggcgtcgt cgagcttcgc
     1441 cggcttgagc aggatctggt cggggatgcc gatcttgccg atgtcgtgca gcggcgcggc
     1501 gagctcgacc gtctcggcct cgtcgtgctc caggccaagc gcgttggcga tcaggcgcga
     1561 gtagcgcgac atgcggatca ggtggttgcc ggtcgcctcg tcgcggaatt cgccggcgcg
     1621 cgcgaggcga agcagcgtct ccttctcgcg ctcgcgcacc tcgcgcgtcg cgtcctccac
     1681 catgtgctcg agcagccgcc tgcggtcctc gagcgcgagt tgctggcgcc tcagggtgag
     1741 gaggttgcgg cagcgcgcga ggcactcgcg cgcatccacg ggcttggtga ggaagtcggt
     1801 gatgccggcg tcgagcgcgg cgtagcgcac cttgcggtcg tcgtgcacgg tgaccatgac
     1861 gatcggcacg tgctcgtagc cgggaagcgc gcgcaggcgc ttcaccaact cgatgccgtc
     1921 catgtcgggc atcatgtagt cgaccagcac cagatcggag gcgtgccgcg tcgcccagac
     1981 caccgcgtcg accgggcgcg cgaagccttg caccgcgacg cgctcatcga ggctgcgcac
     2041 cacctgctcg agaatcgcac ggccggtcga ctggtcgtcg accaccatca cggtattgcg
     2101 cgccgaaggc aggaccctta cttccgccct cgccatgacc tctcctctgg aagctgagct
     2161 atttcacgat gagcctaccc cgcggcgccc tgccctgtcc agcgaccctg gcacatttca
     2221 cgcacttttt cttggcgccg gaagacagac tcgaactctc atgctgttgc cggcggtgga
     2281 ttttgtataa catcgatcgg cttgaggagt atttcggtcg aaccggcaac acagccgtcc
     2341 gaagctgcca cggcgccgaa tgcggaaaca gaccagccat gcaaaggaga ccgcccatga
     2401 ggaagctatt catcccggta gttcttgccg cggcgctgtc cgccggatct gcgggcgcgc
     2461 agatcgtcac caagttgtcg gggatccagc cgctcgagca tccgtcgagc tacgccgaaa
     2521 agttcttttc ggagcaagtc tccattctga ccaacggcgc gatcaaggtc gagaattatc
     2581 acaatactca gctcggtgat gccgtcgcca acgtgcaaag cgtgcgcaac ggcacgatcg
     2641 gctttaccgt ggtgtcggcc tcgaacctta accaggtggt tcccgcgatg gacatgctgt
     2701 cgctgccgtt catcttcaag aacgaggcgc atttctggtg gtacctggcg cagcccgagg
     2761 cggagagctt cgtcaaggcg ctcgaggcca agggcatcaa gaaaatcgcc tatatcgact
     2821 cgggttcgcg caatctgttc acgcagaagg cggtcaggac gcccgcggac ctgaaagggc
     2881 agaagatccg cgtcatggcc tcgccggtga tggtcaagac gatggaagcg ctgggtgcca
     2941 ccggcgttcc ggtggcctgg gccgagctct atacggcgct gcagacaggc gtggtggatg
     3001 gcgccgaaaa caaccacccg tcggtggtgg cgaagaagtt ctacgaagtc tccaagttct
     3061 acacgctgga cgagcacatg cgcattcccg acctgctggt ggtgtcgatg aagttctacg
     3121 actccctcaa cacccagcag aaggcggcgg tgatgcaggc cggcgcgctc acccaggctt
     3181 acatgcgcgg cgcctggaag atctcggaga acaaggacct ggccgagctg aagtccaagt
     3241 tcacgcagat catcgagccc aacaagcagc ccttcatgga cgcggtcaag ccgctggtgc
     3301 tcgccgaggc caagcgcctc gaggtggtaa aaacggtgag cttcatcctc gagtccggca
     3361 agaaattctg agcgttgctg aagctgcttg accgggcggt cgcccggacg ctgacggtcg
     3421 tccgggcggt gtgcatcgcc caggccgcgg cgctgttcct cgtcgtcgtg ctggcggtga
     3481 tcggccgcta tgtgttcggc cgctcgctga cctggaccga ggaagtgccg cgctacctgc
     3541 tgatctggat ctccttcctg ggcgcggcgg cgtgcgtggc gcgacgcgag catgtcggct
     3601 tcgacgtgct gttcaacgcg ctgccc
//
LOCUS       DASPXW010000266         3826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_202845, whole genome shotgun sequence.
ACCESSION   DASPXW010000266 DASPXW010000000
VERSION     DASPXW010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202845"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..963)
                     /locus_tag="VEL04_06115"
     CDS             complement(196..963)
                     /locus_tag="VEL04_06115"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT47977.1"
                     /translation="MSAIALEASGVKKVYLKNGRPLEVLDVERFAVREGEFITVIGPS
                     GCGKSTFLHIMGGFIKAQGSIRVYGQEVSGPGPDRGMMFQEFALFPWRSVAGNIAWGL
                     EAQGFPKGRIEETVSQYLEMMGLGEFRNHYPAELSGGMKQRVALARVLAFDPKVLLMD
                     EPFGALDAQTRETMQEELTRLWERTGKTIVFVTHDIEEAVYLGDRVVVLTARPGRIRE
                     EVRIALPRPRDLTIKKSIQCHEYRNYIWDLIRSESAR"
     gene            988..2322
                     /locus_tag="VEL04_06120"
     CDS             988..2322
                     /locus_tag="VEL04_06120"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HYT47978.1"
                     /translation="MGASDRQGVFSRERLGMLVQPDRVHRSVYADAGVFDLEMQRIFG
                     RAWLVLGHDSQVKAPGDYYTTRMGREPVIVVKKENHEIAVLVNRCAHRGATVCAEGRG
                     NIERFVCPYHGWSYDRGGRLVAVTFESGYDKKNISELKLKAVPRIATYRGFIFASAAA
                     SGPGLEEFLGPARASFDDFVDRAPGGELEVAGGVFKHAYNGNWKLMLENHLDGAHPAY
                     VHASSVAVARAAPEPGAPGEEHYFDIAVRQMRQNGAPDAVWEGTGIWTTPRGHGYMGD
                     YHSDDRLVAGMGNPVFDEYRSRLIEKLGQEAADRVLRVTLWNTIVYPNCSFMSQFRQM
                     RIIQPLAVDRSVVLTYSFRMKGAPPQMFRDTVAFANVVNGTGSWVLTDDLEVYERLQR
                     GLSSGAVDWVYIGRGYGRDIDEAGDVRRGATGTSEVFIRAQMRAWLDYMTAQ"
     gene            2319..2777
                     /locus_tag="VEL04_06125"
     CDS             2319..2777
                     /locus_tag="VEL04_06125"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HYT47979.1"
                     /translation="MSPADFLHHEARLLDTQRFEEWLELFTDDATYWVPLQQNQQDAL
                     ETSSLIHDDRTLLELRVKQLRHPRAHARLPLARTVHQVSNVLLLKESPTDLVVASTLT
                     LIEFRNEKQRLWAALVEHRLRRTPAGLKIAHKRVDLVNSEGELDGIAILL"
     gene            2783..>3826
                     /locus_tag="VEL04_06130"
     CDS             2783..>3826
                     /locus_tag="VEL04_06130"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HYT47980.1"
                     /translation="MKPIVIAGGGIGGLAAALGLARKGFRSIVLEQAPAFGEIGAGIQ
                     LAPNAWHAIDALGVGELVKKEAVFIERLLMMDGVSGERVIDIPLDKRFAKRFGNPYAV
                     THRADIHGALLDGCKAQPSIELRAGTRVSGFEAHADQVTVRTAAGERIEAVALIGADG
                     GRSVVRERIVGDGEPPVSGHMCYRAVLKVDEMPKDLRWAAATLWAAHNTHIVHYPLRG
                     WKLFNLVATVVREQAGTGHNEDAPPSEVLPLFAHNCDKPMSILRVPKVFKRWMLHFRE
                     PVDNWTQGRVTLLGDAAHFMLQYLAQGAAMALEDAVCLAACAEAADGDFAQAFQSYQE
                     KRLVRASRVQMSAS"
BASE COUNT          663 a         1258 c         1258 g          647 t
ORIGIN      
        1 cacagttcaa ccttctgcct cggcgtgaac ctgatccggc ttacgcgggc catcccctcc
       61 atacgcatcc ctcctcatct ggcaagataa gaggtgttgc gttgatcggt tgaacccacc
      121 gtcgaaagcg gtcattccgc caccttcaaa aaagctccac ccttagtaag ccattctgtc
      181 gaagctaatg agtccttacc gcgccgactc gctgcggatg aggtcccaga tgtagttgcg
      241 gtactcgtgg cactggattg atttcttgat ggtgaggtcg cgcggccgcg gcagcgcgat
      301 cctgacctcc tcgcggatgc gccccgggcg cgcggtcagc acgacgacgc gatcgccgag
      361 gtagaccgcc tcctcgatgt cgtgggtgac gaacacgatg gtcttgcccg tccgctccca
      421 cagccgggtc agctcctcct gcatggtttc gcgcgtctgg gcgtcgaggg cgccgaaggg
      481 ctcgtccatc aagagcacct tggggtcgaa ggcgagcacg cgagcaagcg ccacgcgctg
      541 cttcatcccg cccgagagct cggcgggata gtgattgcgg aactcaccga ggcccatcat
      601 ctcgaggtac tggcttacgg tttcctcgat cctgcctttg ggaaaaccct gtgcttcgag
      661 cccccaggcg atgtttccgg ccacgctgcg ccaggggaac agggcgaact cctggaacat
      721 catgccgcgg tcggggccgg gcccgctgac ctcctggccg tagacgcgga tgctgccctg
      781 cgccttgatg aagccgccca tgatgtgcag gaaggtgctt ttgccgcagc cgctcggccc
      841 gatcacggtg atgaactcgc cttcgcgcac ggcgaagcgt tcgacgtcga gcacttccag
      901 cgggcggccg ttcttgaggt agaccttttt gacgcccgaa gcctccaggg caatcgcgct
      961 catggcgata cctttaccat atgcaccatg ggagcgtccg accgccaggg agtgtttagc
     1021 cgcgagcggc tcggcatgct ggtgcagcct gaccgcgtgc accgctcggt gtatgccgac
     1081 gccggggtgt tcgatctcga gatgcagcgc atcttcggcc gcgcctggct ggtgctgggg
     1141 cacgacagcc aggtcaaggc gccaggcgac tactacacca cccgcatggg gcgggaaccc
     1201 gtcatcgtgg taaagaaaga aaaccatgaa atcgccgtgc tcgtgaatcg ctgcgcgcac
     1261 cgcggcgcca cggtatgcgc cgagggacgg ggtaatatcg agcgcttcgt ctgcccgtat
     1321 cacggctgga gttacgaccg cggcgggcgt ctcgtcgcgg tgacgttcga atcgggatac
     1381 gacaagaaaa atatttctga attgaaactc aaggcggtcc cccgcatcgc tacgtaccgc
     1441 ggcttcatct tcgcctcggc cgcggcgagc ggaccgggcc tggaggagtt cctcgggccg
     1501 gcgcgcgctt cgttcgacga cttcgtcgat cgcgcgcccg gcggcgagct cgaggtcgcg
     1561 ggcggcgtgt tcaagcacgc ctacaacggc aactggaagc tgatgctcga gaaccatctc
     1621 gacggcgcgc atccggctta tgtgcacgcg tcctcggtcg cggtggcgcg tgccgcaccc
     1681 gagccgggag cgccgggcga ggagcactat ttcgacatcg cggtgcggca gatgcggcag
     1741 aacggcgccc ccgacgcggt gtgggaagga accggcatct ggaccacgcc gcgcggccat
     1801 ggctacatgg gcgactatca ctcggacgac cgcctcgtcg ccgggatggg caacccggtg
     1861 ttcgacgaat acagatccag gctgattgaa aaactggggc aggaggcagc cgatcgcgtg
     1921 cttcgcgtca cgctgtggaa caccatcgtc tacccgaact gctcgttcat gagccagttc
     1981 cgccagatgc gcatcatcca gccgctcgcg gtcgaccgct cggtggtgct cacctattcc
     2041 ttccgcatga agggcgcgcc gccgcagatg ttccgcgaca cggtggcgtt cgccaacgtg
     2101 gtgaacggca ccggctcctg ggtgctgacc gacgacctcg aggtctacga gcggttgcag
     2161 cgcgggctgt cgtcgggcgc cgtggactgg gtgtatatcg gccgcggcta cgggcgcgac
     2221 atcgacgaag ccggggacgt gcggcgcggc gcgaccggca ccagcgaggt gttcatccgc
     2281 gcccagatgc gcgcctggct cgactacatg accgcgcagt gagtcccgcg gattttctgc
     2341 atcacgaggc gcggctcctc gacacgcagc gcttcgagga atggctcgag ctgttcaccg
     2401 acgacgcgac ctactgggtg ccgctgcagc agaaccagca agacgcgctc gagacctcct
     2461 cgctgatcca cgacgaccgc acgctgctcg agctgcgcgt gaagcagctg cgtcacccgc
     2521 gcgcgcacgc gcgcctgccg ctcgcgcgta cggtgcacca ggtcagcaat gttcttctat
     2581 taaaagagtc ccctaccgac ctggtcgtcg cctcaacgct caccctgatc gaatttcgca
     2641 atgagaagca aaggctgtgg gccgcgctgg tcgagcaccg cctgcgccgc accccagcag
     2701 gcctgaagat cgcgcacaag cgcgtcgatc tcgtaaactc cgaaggcgag ctggacggca
     2761 tcgccatcct gctttgagag acatgaaacc catcgtgatt gcgggcgggg gcatcggcgg
     2821 gctcgcggcc gcgctcggcc tcgcccgcaa gggcttccgc tcgatcgtgc tggagcaggc
     2881 gcccgcgttc ggcgagatcg gcgccggcat ccagcttgcg ccgaacgcct ggcacgccat
     2941 cgacgcgctc ggcgtcggcg agctggtgaa gaaggaggcg gtgttcatcg agcggctgct
     3001 gatgatggac ggcgtcagcg gcgaaagagt gatcgacatc ccgctcgaca agcgcttcgc
     3061 gaagcgcttc ggcaatccct atgcggtgac ccaccgcgcg gacatccacg gcgcgctgct
     3121 cgacggctgc aaggcgcagc cttcgattga actacgtgca ggaacgcgcg tcagcgggtt
     3181 cgaagctcac gctgatcagg tcactgtaag aaccgcggcc ggtgaacgca tcgaagcggt
     3241 cgcactgatc ggcgccgacg gcggccgctc ggtggtgcgc gagcgcatcg tgggcgacgg
     3301 cgagccgccg gtgtcagggc acatgtgcta ccgcgcggtg ctcaaggtcg acgagatgcc
     3361 gaaggacctg cgctgggccg cggcgacgct ctgggccgcg cacaacaccc acatcgtgca
     3421 ttacccgctg cgcggctgga agctcttcaa cctcgtcgcc accgtggtgc gcgagcaggc
     3481 aggcaccggc cacaacgagg atgcgccgcc ctccgaagtg ctgccgctct tcgcccacaa
     3541 ctgcgacaag ccgatgagca tcctgcgggt gccgaaagtg ttcaagcgct ggatgctgca
     3601 ttttcgcgag ccggtcgaca actggacgca gggccgcgtc acgctgcttg gcgacgcggc
     3661 gcatttcatg ctgcagtacc tggcgcaagg cgcggcaatg gcgctcgaag acgcggtctg
     3721 cctcgcggcc tgcgccgagg ccgccgatgg agatttcgca caggcgttcc agagctacca
     3781 ggaaaagcgc ctggtgcgcg cctcgcgcgt gcagatgtcg gcgagc
//
LOCUS       DASPXW010000267         4418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1087831, whole genome shotgun sequence.
ACCESSION   DASPXW010000267 DASPXW010000000
VERSION     DASPXW010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087831"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VEL04_06135"
     CDS             complement(<1..379)
                     /locus_tag="VEL04_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007731483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HYT47981.1"
                     /translation="MELPQNPFKRALRAARAQIGLWSSLSSNYTVEVIAGAGFDWILL
                     DTEHSPSELENLLTQLQAAAPYATHPVVRVAWNDMVTIKRVLDVGAQSLLVPYVSSAA
                     EAKNAVSYTRYPPCGVRGLAGTTR"
     gene            complement(385..1341)
                     /locus_tag="VEL04_06140"
     CDS             complement(385..1341)
                     /locus_tag="VEL04_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYT47982.1"
                     /translation="MKILAATLLALAASVAGAQTYPSKPIRVIVPYAPGGTSDILARQ
                     IGPKLTDAWGQPVIVENKPGANGNVGADLVAKSPADGYILLLTDVGGLVISASVYPKL
                     PFDPSKDFSPIVMVSYSPHVLAVHPSVPVKSVKELIDMAKAQPGKLNFAISGIGGAPQ
                     LAGIEFAQRTGVQWTYIPYKGGSDAVAAVAGGQADVLFNGMLATWPSVQGGRLRALAI
                     SSAQRVAAAPDTPTVAEQGLPGFETGSFQGIVGPAGMPRETVAKLNAELAKVLNLADM
                     KERFAKQGTELRAGTPESFGTWLRNEQARWARVVKQSGAKFD"
     gene            complement(1350..3080)
                     /gene="araD"
                     /locus_tag="VEL04_06145"
     CDS             complement(1350..3080)
                     /gene="araD"
                     /locus_tag="VEL04_06145"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinonate dehydratase"
                     /protein_id="HYT47983.1"
                     /translation="MPQKKRPEELRSYRWYGAQDLRAFGHRSRTAQMGYDRSDYAGKP
                     VIAIINTWSDINSCHTHFKQRVEEVKRGVWQAGGFPVEMPAMSLAEVMQKPTTMMYRN
                     FLAMETEELLRSYPADGAVLMGGCDKTTPALVMGAISMNLPAIYMPAGPMLAGHWREN
                     TLGSGSDTWKYWAELRAGNITERDWKEIEDGIARSPGTCMTMGTAATMMSITEALGLA
                     LPGASSIPAPDSNHAKMATLCGKRAVEMVWEDLKPRDILKKESFDNGIVTLMAMGGST
                     NALIHLVAMAGRAGIKLPLERYNEYSAKTPLLANVRPSGDKYLMEDFYYAGGLRALLS
                     ELRDLLKLDCRTVNGKTLGENLDGARIYNEDVIRTRANPLKTSGGLVVVRGSLAPNGA
                     VIKAAAASPRLLKHTGKAVVFDDYNDMAARIDRDDLDADADSVLVLRNAGPLGGPGMP
                     EWGMLPVPKKLLKQGVRDMVRVSDARMSGTSYGTCVLHVAPESYVGGPLALVGNGDLI
                     ELDVDKRVLNLKVPEAELQKRKGAWKTPPRKFERGYGAIFSQHIRQADEGCDFDFLEG
                     TARIAEPEIH"
     gene            3146..4210
                     /locus_tag="VEL04_06150"
     CDS             3146..4210
                     /locus_tag="VEL04_06150"
                     /inference="COORDINATES: protein motif:HMM:NF007504.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HYT47984.1"
                     /translation="MVFKKGSLERAIEAIVDAGGSEPREARMVAENLVMANLSGHDSH
                     GIGMMPRYVEALQEGGLVVNRKPQTKLDAGALLVLDGGMGYGQTIGYEATRMAITRAK
                     QHGSAIVALGNSHHLGRIGHWAEMAVAAGLISMHFVNVQSFARVAPFGGADRRFGTNP
                     VCIGIPLPGEPPFLLDMATSAVAQGKLRVAHNKGVKIPPGWLIDDNGNPTDDPRWGVI
                     QPFGAMLTFGEHKGYGLAVACELLGGALTGGGVTDYENKTQRRVLNGMLSILIDPQRL
                     GTMEAFARDTRSFLKWLRESRPAPGSDRVRIAGEPERESRAKREREGIPVDQETWNEI
                     RAAAVKLRVAPERIDALARG"
     gene            complement(4207..>4418)
                     /locus_tag="VEL04_06155"
     CDS             complement(4207..>4418)
                     /locus_tag="VEL04_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47985.1"
                     /translation="EPGVFRSRIDAFLEEIRAQEPAEAGRPPLAPGDPERLCAARRLR
                     EGVPLGTGVIAELNALAAELGIAKL"
BASE COUNT          722 a         1454 c         1454 g          788 t
ORIGIN      
        1 cgcgcgtggt gcccgcgagg ccgcgcaccc cgcagggcgg gtagcgcgta tacgagaccg
       61 catttttggc ttcggcggcg ctcgacacgt agggcacaag cagcgactgt gcgccgacgt
      121 cgagcacgcg cttgatcgtc accatgtcgt tccaggccac gcgcaccacg ggatgcgtcg
      181 cgtaaggcgc cgccgcctgc agctgggtga gcaggttttc cagctcgctc ggcgaatgct
      241 cggtgtcgag caggatccag tcgaagcccg cacccgcgat cacctcgacc gtgtagttcg
      301 acgacaggct cgaccacagt ccgatctgcg ccctggcggc gcgcagcgcg cgcttgaacg
      361 gattctgggg aagctccatc gccgtcagtc gaacttcgcc ccggattgct tcaccacgcg
      421 cgcccagcgc gcctgctcgt ttcgcagcca ggtaccgaag ctttcaggcg tcccggcgcg
      481 tagctccgtg ccttgtttcg cgaagcgttc cttcatgtcc gccaagttca gcaccttggc
      541 cagctccgcg ttcagcttcg ccacggtctc gcgcggcatt ccggcgggcc cgacgatgcc
      601 ctggaacgac cccgtctcga agccgggcag cccctgttcg gctaccgtcg gggtatccgg
      661 cgctgccgcg acgcgctgcg cgctggaaat cgccagcgcc ctcagcctgc cgccctgaac
      721 cgagggccag gtggcgagca tgccgttgaa gagcacgtcc gcctgcccgc ccgcgacggc
      781 ggcgactgca tccgatccgc ccttgtacgg aatgtaggtc cactgcacgc cggtgcgttg
      841 cgcaaactcg atgcccgcga gctgtggcgc gccgccgatt ccggaaatgg cgaaattcag
      901 cttgccgggc tgcgctttcg ccatgtcgat cagttccttc acgcttttca cgggcaccga
      961 gggatgcacc gcgagcacgt gcggcgaata ggaaaccatc acgatcggcg agaaatcctt
     1021 cgacggatcg aacggcagct tgggatagac gctggcgctg atcaccaggc cgccgacgtc
     1081 ggtcagaagc aagatatagc cgtcggcggg gctcttggcg accagatccg cgcccacatt
     1141 gccgtttgcg cccggcttgt tctccacgat caccggctgg ccccaggcgt cggtgagctt
     1201 cggcccgatc tgccgcgcga ggatgtccga ggtgccgccc ggcgcgtaag gcacgatgac
     1261 gcggatgggc ttcgaaggat aagtctgcgc gccggcgacc gaggcggcga gggcgagcag
     1321 tgtggcggcg aggattttca tggtcgatgt cagtgaattt ccggctccgc gatgcgcgcg
     1381 gtgccctcta gaaagtcgaa gtcgcagcct tcgtcggcct ggcggatgtg ctgcgagaaa
     1441 atggcgccgt agccgcgctc gaacttgcgg ggcggggttt tccacgcgcc ttttctcttt
     1501 tgcagttctg cctccgggac cttcaggttg agaacccgtt tgtcgacatc gagctcgatg
     1561 aggtcgccgt ttccgaccag cgcgagaggc ccgccgacgt aggactccgg cgcgacgtgc
     1621 agtacgcagg tgccgtagct cgtgccgctc atgcgggcat ccgacacccg caccatgtcg
     1681 cgcactcctt gcttgagaag cttcttcggg acgggaagca tgccccattc cggcataccc
     1741 gggccgccga gcgggccggc gtttctcaaa acgagcaccg aatcggcgtc ggcgtccaga
     1801 tcgtcgcggt caatgcgcgc cgccatgtcg ttgtaatcgt cgaatacgac cgccttgccg
     1861 gtgtgcttca acagcctggg gcttgctgct gcggccttga tgaccgcgcc gttcggcgcc
     1921 aggctgccgc gcacgacgac caggcccccc gaggtcttga gcggattggc ccgcgtgcga
     1981 atgacgtctt cgttgtagat gcgcgcgccg tccagatttt cgcccagcgt ttttccgttg
     2041 acggtccggc aatccagttt caacaaatcc cttaattcac tcaataaagc ccgcagtcca
     2101 cccgcatagt agaaatcctc catcaggtac ttgtcgcccg acggccgcac gttggcgagc
     2161 agcggcgttt tcgccgagta ctcgttgtag cgctcgagcg gcagcttgat acccgcccgg
     2221 ccggccatcg caacaaggtg gatcagtgca ttggtcgatc ctcccatcgc catcagcgtc
     2281 acgatgccgt tatcaaaaga ctcttttttt agaatatccc gaggcttgag gtcctcccac
     2341 accatctcga ccgcgcgctt gccgcaaagc gtcgccatct tcgcgtggtt ggagtcgggc
     2401 gccgggatgg aggatgcgcc cggtagcgcg agtccgagcg cctcggtaat ggacatcatg
     2461 gtcgccgcgg tgcccatggt catgcaggtg ccgggcgagc gcgcgatgcc gtcctcgatt
     2521 tctttccagt cccgctcggt aatgttgccg gcgcgcaatt ccgcccagta cttccacgtg
     2581 tcggagcccg agccgagggt gttttcccgc cagtggcccg cgagcatcgg ccccgcaggc
     2641 atgtagatcg ccggcaggtt catgctgatc gctcccatga ccagcgccgg ggtggtcttg
     2701 tcgcagccgc ccattagcac cgcgccatcg gcgggatagg agcgcagcag ctcctcggtc
     2761 tccatggcga ggaagttgcg gtacatcatg gtggtcggct tctgcatcac ttcggcgagc
     2821 gacatcgcgg gcatctcgac cgggaagccg cccgcctgcc agacgccgcg cttcacttcc
     2881 tcgacgcgct gcttgaagtg cgtgtggcag gagttgatgt ccgaccaggt gttgatgatc
     2941 gcgatcaccg gcttgccggc gtagtcgctg cggtcatagc ccatctgcgc ggtgcgcgag
     3001 cgatggccga aggcgcgcag gtcctgcgcg ccgtaccagc ggtacgaacg cagctcttcg
     3061 gggcgcttct tctgcggcat ggggagagcg gattttagat gcaaaacggc gcgcgcagcg
     3121 ccccctctat aatctgctct tggacctggt tttcaaaaag ggctcgttag aaagggcaat
     3181 cgaagcgatc gtcgatgcgg gcggcagcga gccgcgcgag gcgcgcatgg tcgccgagaa
     3241 cctggtcatg gcgaacctga gtggccacga ctcgcacggc atcggaatga tgccgcgtta
     3301 cgtcgaggcc ctgcaggaag gcggtctggt cgtgaaccgc aagccgcaga ccaagctcga
     3361 cgcgggcgcg ctgctggttc tcgacggggg catgggctac gggcagacca tcggctacga
     3421 ggcgacgcgc atggcgatca cgcgcgccaa gcagcacggg tcggcgatcg tcgcgctcgg
     3481 caactcgcat cacctcggcc gcatcggaca ctgggccgag atggcggtcg cggcaggcct
     3541 catatcgatg cacttcgtga acgtgcaatc ttttgcgcgc gttgcgcctt tcggcggagc
     3601 ggaccgccgc ttcggcacca acccggtgtg catcggcatc ccgctgcccg gcgagccgcc
     3661 cttcctgctc gacatggcga ccagcgccgt agcgcagggc aagctgcgcg tcgcgcataa
     3721 caagggagtg aagatcccac ccggctggct gatcgacgat aacggcaatc cgaccgacga
     3781 tccgcgctgg ggcgtgatcc agcctttcgg cgccatgctc acgttcggcg agcacaaggg
     3841 ctacgggctc gccgtcgcct gcgagttgct ggggggcgca cttaccggcg gcggcgtaac
     3901 cgactatgaa aacaagacgc agcgccgggt cctgaacggc atgctgtcga tcctcatcga
     3961 tcctcagcgg cttggcacta tggaggcttt tgcaagggat acgcgcagct ttctcaagtg
     4021 gctgcgtgaa tctcgccctg cgcccggaag cgaccgcgtg cgcattgcgg gcgagccgga
     4081 gcgggaatcc cgcgcgaagc gcgagcgcga agggattccc gtagaccagg agacctggaa
     4141 cgagatccgc gcggccgcgg tcaagctgag ggtcgcgccg gaacggatcg acgcactggc
     4201 gcgcggttaa agttttgcga tgccgagctc ggcggcgagc gcgttcagct cggcaatgac
     4261 gccggtgccg agcggcaccc cttcgcgcaa gcgccgggcg gcgcacagcc gctcggggtc
     4321 gcccggcgcg agcggcggcc gaccggcttc ggctggctcc tgcgcgcgga tttcctccag
     4381 aaacgcgtcg atccgggagc gaaatacgcc gggctcgg
//
LOCUS       DASPXW010000268         2384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1088049, whole genome shotgun sequence.
ACCESSION   DASPXW010000268 DASPXW010000000
VERSION     DASPXW010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1088049"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VEL04_06160"
     CDS             <1..216
                     /locus_tag="VEL04_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HYT47986.1"
                     /translation="DAVYYSHFKCNLRRLVDYPRLWRYTRRFYALPGVARTVDLAEIK
                     SHYYRSMKHINPTQIVPKGPLLDFRLH"
     gene            222..1511
                     /locus_tag="VEL04_06165"
     CDS             222..1511
                     /locus_tag="VEL04_06165"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HYT47987.1"
                     /translation="MLAAPAALANSDPYTDTLPTGGAAVGYLWQAERSPYRGAQSGHS
                     LDPFYLYEGEHAYLHSSRAGLKLTSEAWRFDAFVRERFEGYTVDRVPSAAVGMPVREP
                     GFDLGAAVRRRTAFGTPYVELLRDASHRSGGSELRLGYWTNPWSRGRLQLRPHAALAY
                     RDAKLNDYYYGAGAGTDVEIAAYATYRMMGQWYLIGSLGAARRSGAIAASPLVENRVN
                     TTAMLGVMYDFSPAIKRWEPESKPLIARLLYGASSDCDVLQIVRLNCTSTHTVDSTDI
                     WAMEIGRTLIKQPNGHPVEIAGFLGLLRHVERGNQADFWQFNAYLKAYYYGLPWDKWL
                     RTRFGIGSGLAYAERVPTMESRDQAKSGLGTWKLLNYLDPTVDVRVSDLLGHAVPRDS
                     YVGIGVSHRSGMFGRSQLLGNVDGGSNYIYVYLETTF"
     gene            complement(1508..2263)
                     /locus_tag="VEL04_06170"
     CDS             complement(1508..2263)
                     /locus_tag="VEL04_06170"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020723049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HYT47988.1"
                     /translation="MTAFDLSGKAALVTGGNGGIGLGMAQGLAAAGAAVAIAGRDEAK
                     SSAALKSLGRKAIGVHADLSDEKACRAAVDEAADKLGRLDILVNNAGINIRKAPQDYT
                     LEEWRLVLDTNLTSAFVASQAAYPHLKRRGGGKIINIGSMMSLFGASFVAPYGASKGG
                     LVQLTRALACAWAKDNIQVNAVLPGWIDTALTRRARKEVAGLHERVLARTPAARWGTP
                     DDLAGIAVFLSSSASDFVTGTAIPVDGGYASQG"
BASE COUNT          396 a          831 c          763 g          394 t
ORIGIN      
        1 gacgcggttt attactcgca cttcaaatgc aacctcaggc gactggtgga ctatcccaga
       61 ctatggcgct acacgcgaag gttctacgcg ctgcccggtg tggcgcgcac ggtcgatctc
      121 gccgagatca agtcgcatta ctaccgcagc atgaagcaca tcaacccgac gcagatcgtt
      181 cccaagggcc cgctgctcga ctttcgcttg cattaggcct gttgctcgct gcgccggcgg
      241 cgctggcaaa cagcgatccc tataccgaca ccctgccgac gggcggcgcc gcggtcggct
      301 acctgtggca ggccgagcgc tcgccctacc gcggggcgca aagcgggcac agcctcgacc
      361 ctttttatct gtacgaaggt gagcacgcct acctgcactc ctcgcgcgcc ggcctcaagc
      421 tcacgtcgga ggcctggcgc ttcgacgcct tcgtcaggga gcgcttcgag ggctacacgg
      481 tggatcgcgt gccttcagcc gccgtcggaa tgccggtgcg cgagccgggc ttcgacctcg
      541 gcgccgcggt gcgccgccgc accgcattcg gcacgccata cgtcgagctg ctgcgtgacg
      601 cgagccaccg ctcgggaggc agcgagctgc ggctcgggta ctggacaaac ccctggtcgc
      661 gcggccgcct gcagctgcgc ccgcatgccg cgcttgctta ccgcgatgcg aagctgaatg
      721 actactacta cggcgcggga gcgggcacgg atgtggaaat cgcggcctat gcgacctacc
      781 gcatgatggg ccagtggtac ctcatcggca gcctaggcgc ggcgcggcgc tcgggcgcaa
      841 tcgccgcgag cccgctggtc gagaaccgcg taaacaccac ggcgatgctc ggcgtaatgt
      901 acgacttttc gcccgccatc aagcgctggg agcccgagtc gaagcccttg atcgcgcggc
      961 tgctgtatgg cgcctcgagc gactgcgacg tgctgcagat cgtgcgcctc aactgcacct
     1021 ccactcacac cgtcgacagc accgacatct gggcgatgga gatcggccgt acgctgatca
     1081 agcagcccaa cggccatccg gtcgagatcg ccggcttcct cggcctgctg cggcacgtgg
     1141 agcgcggcaa ccaggcggat ttctggcagt tcaacgcgta cctcaaggcc tactactacg
     1201 gcttgccgtg ggacaaatgg ctgcgcacgc gcttcggcat cggcagcggc ctggcgtacg
     1261 cggagcgagt accgaccatg gagagccgcg accaggcgaa aagcggcctc ggcacctgga
     1321 agctcctcaa ctatctcgat ccaaccgtcg acgtgcgcgt gagcgacctt ctgggccacg
     1381 cggtgccacg cgacagctac gtcggcatcg gcgtctcgca ccgctccggg atgttcggcc
     1441 gctcgcagct cctcggcaac gtcgacggcg gctccaacta catctacgtc tatctcgaga
     1501 cgacgttcta gccctgcgag gcgtagccgc cgtcgaccgg gatggcggtg ccggtgacga
     1561 agtccgaagc ggaagagctc aggaacaccg cgatcccggc gagatcgtcc ggcgtccccc
     1621 agcgcgcggc gggggtgcgc gcgagcacgc gctcatgcag gcccgcgacc tccttgcgcg
     1681 cgcggcgcgt gagcgcggtg tcgatccagc cgggaagcac cgcgttcacc tggatgttgt
     1741 ccttcgccca ggcgcaggcg agcgcgcgcg tgagctgcac gaggccgcct ttgctcgcgc
     1801 cgtagggcgc gacgaaagag gcaccgaaca gcgacatcat cgagccgatg ttgatgatct
     1861 tgccgccgcc gcggcgcttc aagtgcggat acgccgcctg cgaggcgacg aacgcgctcg
     1921 tcaggttggt gtcgagcacc agccgccatt cctccagcgt gtagtcctgc ggcgccttgc
     1981 ggatgttgat gccggcgtta ttcaccagga tgtcgagccg gccgagcttg tctgccgcct
     2041 cgtcgaccgc ggcgcggcag gccttctcgt cgctgaggtc agcatggacc ccgatcgctt
     2101 tccttcctag cgacttcaga gctgcagaac tcttcgcctc atccctgccg gcaatggcga
     2161 ccgcggcgcc ggcagcagcc aggccctgcg ccatgcccag gccgatgccg ccgttgccgc
     2221 cggtgaccag cgccgccttt cccgacagat cgaaggccgt catcgaatta tttgacaaaa
     2281 tcaagtatcg cgccgagact cgccgcatgc cgcagatcga ggccgtgcgc cgcttcaacc
     2341 gattctatac gcgccgcatc ggcgtcctga agccgggcat ggtc
//
LOCUS       DASPXW010000269         2383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1464765, whole genome shotgun sequence.
ACCESSION   DASPXW010000269 DASPXW010000000
VERSION     DASPXW010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1464765"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VEL04_06175"
     CDS             <1..470
                     /locus_tag="VEL04_06175"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HYT47989.1"
                     /translation="FPLGLFRAWSHVEPDARCLVYPRPERAPLPPYSGEAAAGALRSP
                     TPGNDDFSGLRGYQLSDSPRHVAWKAVARSHDMLTKQFTGEAAAELWLDWRLLPAAMA
                     LENRLSRLAGWVLAAERSGIVYGLRLPGVELAPARGDAHRADCLQALALYRLP"
     gene            509..>2383
                     /locus_tag="VEL04_06180"
     CDS             509..>2383
                     /locus_tag="VEL04_06180"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HYT47990.1"
                     /translation="MSTRDLVYLTGSLALVILPHALRAPWWLTLLTLCLYGWRFYFSL
                     NRAPLPSRWLVLGIAAVAMLALWVEYRTLFGRQPGILLLMLFSGVKLLETRTHRDAAV
                     AAFLAYFLIITNFLYSQSVVTAALMGAALFAITATLVGFSAPQRAPRANVRTAGVLLA
                     QAVPAALALFLLFPRVPGPLWGLPQDAYAGITGLSETMSPGNLASLAQSDAIAFRAEF
                     DGAPPLHALRYWRGPVMWDFDGRTWSIGADYRADASLPQGGRATYRYGVILEPHNRTW
                     LFALETAASVPARARLSFDGQLLALAPVRSRMRYDLVSVIAPEPRLSEVRGALSRALR
                     LPPGFNPRAVTLAHQWRAASASDAEVVARAVEFLKAGRYAYTLEPPLLGADSVDEFLF
                     DTKAGFCEHFASAFAFLMRAAGIPARVVTGYQGGELNTVDRIVTVRQTDAHAWTEVFL
                     NGRGWTRVDPTAAAMPRRVEAGLARAVPETEPLPLMMRARLEWLRGVRFQWEALAHKW
                     NVWVLGYNLDRQRDLMRSVGMPDADWRSLTAALFTILGVITMGLLAWSLKRLARPDPV
                     QKAWRAFCRKLATRGVERAPHEGPRDYATRAARALPAARRSILHIGALYVGLRYGAHA
                     S"
BASE COUNT          285 a          893 c          811 g          394 t
ORIGIN      
        1 gctttccgct tggcctgttc cgcgcctgga gccatgtgga gcccgatgcg cgctgcctgg
       61 tctacccgcg acccgagcgc gcgccgctgc cgccatacag cggcgaggca gcggccggcg
      121 cgctgcgctc gccgactccg ggcaacgacg atttttccgg cctgcgcggc tatcagctct
      181 cggattcgcc gcgccatgtc gcctggaagg cggtggcgcg cagccacgac atgctgacca
      241 agcagttcac cggcgaggct gcggccgagc tatggctcga ctggcggctg ctccccgcag
      301 ccatggcgct cgagaaccgg ctgtcgcggc tcgccggctg ggtgctcgct gccgagcgct
      361 cgggcatcgt gtacggattg cgcctgccgg gcgtcgagct cgccccggcg cgcggcgacg
      421 cgcaccgcgc ggactgcctg caagcgctcg cgctgtaccg gctgccgtga gccccgcgcc
      481 gcatccgctc gccccggagc agcgccccgt gagcacgcgt gacctggtgt acctgaccgg
      541 ctcgctcgcg ctcgtgatcc tgccgcacgc cctgcgcgcg ccgtggtggc tgacgctgct
      601 cacgctctgc ctgtacggct ggcggttcta cttctcgctg aatcgcgcgc cgctgccctc
      661 gcgttggctg gtgctcggca tcgctgcggt cgcgatgctc gctctgtggg tcgagtaccg
      721 cacgcttttc ggcagacagc cgggaatcct gctcctgatg ctgttctcgg gcgtgaagct
      781 gctcgagacg cgcacgcacc gcgacgcagc agtcgccgcc ttcctcgcct attttctgat
      841 tatcaccaac tttctctaca gccagtcggt cgtcacggca gcgctgatgg gcgcagcgct
      901 gttcgccatc accgctacgc tggtcggctt cagcgccccg cagcgcgcac cgcgcgccaa
      961 cgtgcgcacc gcgggggtcc tgctcgctca agccgtgccc gccgcgctcg ccctgtttct
     1021 cctctttccg cgcgtgcctg gacccctttg ggggctgccg caggatgctt atgccggaat
     1081 caccggcctg tccgagacga tgagcccggg caacctcgca agcctggcgc agtcggacgc
     1141 catcgccttc cgcgccgagt tcgacggcgc gccgccgctg cacgcgctgc gctactggcg
     1201 cggtccggtg atgtgggact tcgacggccg cacctggagc atcggcgcgg actatcgggc
     1261 cgatgcgtcg ctgccccagg gaggccgggc gacctaccgc tacggcgtga tcctcgagcc
     1321 gcataaccgc acctggctgt tcgcgctcga gactgcggcc agcgtgcccg cgcgcgcgcg
     1381 cctgagcttc gacggccagc tgctcgccct cgcgccggtg cgcagccgca tgcgctacga
     1441 cctcgtgtcg gtcatcgccc ccgagccgcg actttccgag gtgcgggggg cgctctcccg
     1501 tgccctgcgc ctgccgccgg gcttcaaccc ccgcgccgtc acgctggccc accagtggcg
     1561 cgcggcatcg gcttccgacg ccgaggtggt cgcgcgcgca gtcgagttcc tcaaggcggg
     1621 gcgttatgcc tatacgctcg agcctcccct gctcggcgcc gattcggtcg acgagttcct
     1681 gttcgacacc aaagccgggt tctgcgagca cttcgcatcc gcgttcgcct ttctgatgcg
     1741 cgcagccggg ataccggcgc gcgtcgttac cggctaccag ggcggcgagc tcaacacggt
     1801 ggaccgcatc gtcacggtgc gccagaccga cgcgcacgcc tggactgaag tatttctcaa
     1861 cggacgcggc tggacgcggg tcgaccccac cgccgcggcg atgccacggc gcgtcgaagc
     1921 cggcctcgcg cgcgccgtgc ccgagaccga gccgctgcct ctgatgatgc gagcgcgcct
     1981 ggagtggctg cgcggcgtgc gctttcaatg ggaggcgctg gcgcataaat ggaacgtctg
     2041 ggtgctgggc tataacctcg accggcagcg cgacctgatg cgctcggtcg gcatgcccga
     2101 cgccgactgg cgctcgctca cggcggcgtt gttcaccatc ctcggcgtga tcacgatggg
     2161 gctgctcgcg tggtcgctga agcgcctggc gcggcccgac ccggtgcaga aggcctggcg
     2221 cgcgttctgc cgcaagctcg cgacgcgcgg cgtcgagcgc gcgccgcacg aaggaccgcg
     2281 cgactacgcg acgcgcgccg cgcgggcgct gccggcggcg cgccgcagca tcctgcacat
     2341 cggcgcgctg tacgtcggcc tgcgttatgg cgcgcacgcc agc
//
LOCUS       DASPXW010000270         2524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_832722, whole genome shotgun sequence.
ACCESSION   DASPXW010000270 DASPXW010000000
VERSION     DASPXW010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832722"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="VEL04_06185"
     CDS             complement(<1..852)
                     /locus_tag="VEL04_06185"
                     /inference="COORDINATES: protein motif:HMM:NF019932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HYT47991.1"
                     /translation="MRRPILLLMAGALSACAADSIQTFPLPEVANPAQASEVTFIRPR
                     GLVASEFPFYITLGERPVFDLRNGENTRFRIAPGRQSIAMRCLGGPASKPAETRIDRE
                     FTPGSAAFFVVEPKFECASIEPVDAQAAAALTRRTRFRAVGTVTRLAEMSGAPSSAVT
                     TTPAPPAVVAAPADPKDQVAAATASWIEAFNSRDPVRLTALYDPEAVLSGTAAQKPIT
                     GAAIGEYFRNAMQRPANRASLGEHQVRVYGDTAIDTGSYTFSEVRDGSVAAVLPERYT
                     LVYRNRGG"
     gene            complement(868..1374)
                     /gene="thpR"
                     /locus_tag="VEL04_06190"
     CDS             complement(868..1374)
                     /gene="thpR"
                     /locus_tag="VEL04_06190"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HYT47992.1"
                     /translation="MRLFFALWPPAATARALEDWTEKLRKQTGGKPMRAENIHLTLAF
                     LGEAEPAKAIAAAQRVRGAPHALPIEQALHWKHNDIVWAGPRATPAELQDLVKQLHQA
                     LSDESFILEKRPFAAHVTLLRKARAPKLLPPLPRAEWPVDEFLLVRSHTVREGSTYEP
                     LQRFTLVR"
     gene            complement(1371..1988)
                     /locus_tag="VEL04_06195"
     CDS             complement(1371..1988)
                     /locus_tag="VEL04_06195"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HYT47993.1"
                     /translation="MYSPPYNRVEKRAAVLEFMRANNFALLVTGKNDALHASHLPVML
                     HEEKGQIILDMHMARANAQWREFSGAEVLVAFFGPHAYVSPRWYEEKERVPTWNYAAV
                     HAYGIPEIISEKEVKRASQRRLIESLDAQWLPQFDALREEYVQRMLGAIVNFRIPVAR
                     LETRWKLSQNRGRREQELIAAELEKSSDPTERALAALTRKHLASE"
     gene            complement(1990..2457)
                     /gene="ispF"
                     /locus_tag="VEL04_06200"
     CDS             complement(1990..2457)
                     /gene="ispF"
                     /locus_tag="VEL04_06200"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HYT47994.1"
                     /translation="MRVGQGFDVHAFVGGRKLVIGGVEIPHEKGLDGHSDADVLLHAI
                     CDALLGAAGLGDIGQHFPDYDPKYAGIDSRGLLKDVAKKIRTLNFRIINVDATILAQA
                     PRMAPHIPRMIGNIAADLGLAPAAINVKATTTERLGFLGRGDGIAAQAVVLID"
BASE COUNT          388 a          827 c          864 g          445 t
ORIGIN      
        1 gccgccgcga ttgcggtaca ccagcgtgta gcgttccggc agcaccgccg cgacgctgcc
       61 gtcccgcacc tccgaaaacg tgtagctgcc ggtgtcgatg gcggtgtccc cgtagacgcg
      121 cacctggtgc tcgccgaggg acgcccggtt cgccgggcgt tgcattgcgt tcctgaaata
      181 ctcgccgatc gccgcgccgg tgatcggctt ctgggccgcg gttcccgata gcacggcctc
      241 cgggtcgtag agcgcggtga ggcgcaccgg atcacggctg ttgaacgcct cgatccagga
      301 agcggtggcc gccgccacct gatccttggg atcggcgggc gcggcgacga cggcaggcgg
      361 ggcgggcgtc gtagtgacgg cgctcgacgg tgcgccgctc atctcggcga gcctggtcac
      421 cgtgcctacg gcgcggaatc gggtcctgcg ggtgagcgcg gccgccgcct gcgcgtcgac
      481 cggctcgatg ctcgcgcact cgaatttcgg ctcgacgacg aagaacgccg cacttcctgg
      541 agtgaattcg cgatcgatgc gggtctccgc cggcttcgac gccggtccgc cgaggcagcg
      601 catggcgatg gactgcctgc ccggcgcgat ccgaaaccgg gtgttctcgc cgttccgcaa
      661 gtcgaacacc ggccgttcgc cgagcgtgat gtagaacggg aactcgctcg ccaccaggcc
      721 tcggggccgg atgaaggtga cctcgctcgc ctgcgcggga ttcgccacct cgggaagcgg
      781 gaaggtctgg atggaatccg cagcgcatgc agagagcgct ccagccatga gcagcagaat
      841 cgggcgtcgc atgtactttt ccggttgcta ccttaccaga gtaaaccgct gcaacggctc
      901 ataggtcgag ccctcgcgca cggtgtgcga gcgcaccagc aggaattcgt cgaccggcca
      961 ctcggcccgc ggcaagggcg gcagcaattt cggcgcgcga gccttgcgca gcagcgtgac
     1021 gtgcgcggcg aaggggcgct tctcgaggat gaatgattca tccgacagtg cctgatggag
     1081 ctgcttcacc aggtcctgca gctcagcggg cgtggcccgg ggcccggccc agacgatgtc
     1141 gttgtgcttc cagtgcagcg cctgctcgat cggcagcgcg tgcggcgcgc cgcgcacccg
     1201 ctgcgccgcg gcgatagcct tggcgggctc ggcctcgccg agaaaagcca gcgtgaggtg
     1261 gatgttttcg gcgcgcatcg gcttgccgcc ggtttgcttg cgcagctttt cggtccagtc
     1321 ttcgagcgcc cgcgcggttg cggctggcgg ccagagggca aagaacagcc tcactccgag
     1381 gcgaggtgct tcctggtgag tgcggcgagc gcgcgttcgg tcggatccga tgacttttcg
     1441 agctcggcgg cgatcagctc ctgctcgcgg cggccgcggt tctgcgacag cttccagcgc
     1501 gtctccagcc gcgccaccgg aatgcggaag ttgacgatgg cgccgagcat gcgctgcacg
     1561 tactcctcgc gcagcgcatc gaactgcggc agccactgcg cgtcgagaga ttcgatgagg
     1621 cggcgctggg atgcacgctt cacctctttc tcggaaatta tttctggaat tccgtatgcg
     1681 tgcaccgctg cgtagttcca ggtcggcacg cgctccttct cctcgtacca gcgcggcgag
     1741 acgtaggcat gcggcccgaa gaacgccacc aggacctcgg cacctgaaaa ctcacgccac
     1801 tgcgcgttgg cgcgcgccat gtgcatgtcg aggatgatct gccccttttc ctcgtgaagc
     1861 atcacgggaa ggtgcgaagc gtgcagcgca tcgttcttcc cggtgaccag cagcgcgaaa
     1921 ttattcgcgc gcatgaactc gagcactgcc gcgcgcttct ctacccggtt gtagggcggc
     1981 gagtacatct cagtcgatca gcactacagc ctgcgcggcg atgccgtcgc cgcggccgag
     2041 gaagccgagt cgctcggtgg tggtcgcctt gacgttgatc gcggcgggcg cgagcccgag
     2101 gtcggcggcg atgttgccga tcatgcgcgg aatgtgcggc gccatgcgcg gcgcctgcgc
     2161 aagaatggtc gcatcgacgt tgatgatcct gaagttcagg gttctgatct ttttcgccac
     2221 atccttcagc agcccgcggc tgtcgatgcc ggcgtacttg ggatcgtagt cgggaaagtg
     2281 ctggccgatg tcgccgagcc cggccgcgcc gagcagcgcg tcgcagatcg cgtgcagcag
     2341 cacgtcggcg tcggaatgcc cgtcgaggcc tttctcgtgc ggaatctcga cgccgccgat
     2401 gacgagcttt ctgccgccga cgaaggcgtg cacgtcgaat ccctgcccga ccctcatagg
     2461 gggcacctca tttacgtccc gacaggcatg cctcggcgag cgcgaggtcc tcgggaaacg
     2521 tcac
//
LOCUS       DASPXW010000271         2426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_583188, whole genome shotgun sequence.
ACCESSION   DASPXW010000271 DASPXW010000000
VERSION     DASPXW010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583188"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..409
                     /locus_tag="VEL04_06205"
     CDS             110..409
                     /locus_tag="VEL04_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT47995.1"
                     /translation="MDSKTLQVDVEAKMKALFARCPTLHGFSVQDRGMLPRDVAQNRI
                     PDADLFITEIGVFPKLDSQDGEIHDEITLAISDLVQDQPRACDYLRGRTFARTLH"
     gene            complement(480..1004)
                     /locus_tag="VEL04_06210"
     CDS             complement(480..1004)
                     /locus_tag="VEL04_06210"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HYT47996.1"
                     /translation="MKMQNAAPRLAAIAFALAGVAFALPQAYAAGEGDAQPTSQPSEK
                     FRKLDRNSDGFLDKGEARHMRGYTNAFDQADENRDGKLDPSEFIKAESIYERQQIAKY
                     VDDGVLTTKVKAALLKEPNLKSIDVHVETYQGKVLLSGWVDSAAQRERAVKAASSVQG
                     VAAVKDGLEIKATK"
     gene            complement(1114..2064)
                     /locus_tag="VEL04_06215"
     CDS             complement(1114..2064)
                     /locus_tag="VEL04_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018314187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HYT47997.1"
                     /translation="MKRILLLWLFTASAGAQVSVQEYPIPSGHRVHDVWADAAPGGPV
                     WFSAQGSGHLGILDPKSGKVEFVALGSGSSPHGVIAGEDGAPWLTDGGQNAIVRVDPK
                     TRQVKRWKLPEGSGYTNLNTAIFDKQGIHWFTGQSGIYGSLDPKSGDMKLFDAPKGRG
                     PYGIHATPDGTVYYASLAGSFLGKINSINGATTVIEPPTRRQGARRVWSDSKGHIWVA
                     EWNSGNLSRYEPASGKWSMWQAPGDDPHVYAVYVDETDKVWVSEWTAQAMLRFDPATA
                     KFESFRSSSRSANVRQIHGRRGEVWTPESAADKIVVYRYK"
     gene            complement(2135..>2426)
                     /locus_tag="VEL04_06220"
     CDS             complement(2135..>2426)
                     /locus_tag="VEL04_06220"
                     /inference="COORDINATES: protein motif:HMM:NF020253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HYT47998.1"
                     /translation="DALKKLFKSEPKRKLVGVEIEGGRLDLNMTRWLVRGGGHVTSAV
                     YSPRLRKNIGYLMLPIDRVSEKNLQVETADGVRAATVVAMPFVDPKKQLAKS"
BASE COUNT          383 a          796 c          769 g          478 t
ORIGIN      
        1 ccgaagagct tggcgagttg ctgcgcggcc ataccttcgc tcggaccgtg cactaattca
       61 tagcttcagg tttcgcgtcc gtgtagtgtt aatggtgaca atggactcga tggactcgaa
      121 aacgctccag gtggatgtcg aggcgaagat gaaggcgctg ttcgcgcgct gcccgacgct
      181 gcacggcttc tcggtgcagg atcgcggcat gctgccgcgc gatgtcgccc agaaccgcat
      241 cccggacgcc gatctcttca tcaccgagat cggcgtgttt ccgaagctcg actcccaaga
      301 cggcgaaatt cacgacgaga tcacgctcgc gatctcggat ctcgtgcaag accagcctcg
      361 cgcctgcgat tacctgcgcg ggcgcacctt cgcgcgcacg ctgcactgaa gaaaagacag
      421 gcggaggaaa tgacgctcgc cgccgcggcg acttgcgtcg ccgcggcggc ttgagcgcgc
      481 tacttggttg ccttgatttc gaggccgtcc ttcactgcag cgacgccctg cacggacgag
      541 gcagctttca ctgcccgttc gcgctgcgcg gcgctgtcga cccagcccga gagcaggacc
      601 ttgccctggt aggtctcgac atgaacgtcg attgacttga ggttcggctc cttcagcagg
      661 gcggccttca ccttggtggt gagcacgccg tcgtcgacgt acttggcgat ctgctggcgc
      721 tcgtagatcg actcggcctt gatgaactcg ctcgggtcga gcttgccgtc gcggttctcg
      781 tcggcctggt cgaaggcgtt ggtatagccg cgcatgtggc gcgcctcgcc tttgtcgagg
      841 aagccgtcgc tgttgcggtc gagcttgcgg aacttctcgc tcggctgcga ggtcggctgc
      901 gcgtcgccct cgccggcggc gtaagcctgc ggcagggcga acgcgacgcc cgcgagggcg
      961 aacgcgatcg ctgcgagacg cggtgctgcg ttttgcatct tcatgaggta ctcctttctc
     1021 accggatggc ggtgatgcgg atttgaccgg cgtatccgca gagcgttgca tcaagttttg
     1081 tcgcctggcg gcgacacaaa ctacaattgc gccctacttg tacctgtaga cgacgatctt
     1141 gtcggccgcc gactcgggag tccagacctc gcccctgcgg ccgtggatct gccgcacgtt
     1201 cgccgagcgg ctcgaagacc tgaagctctc gaacttcgcg gttgccgggt cgaagcgcag
     1261 catcgcctgc gcggtccact ccgagaccca caccttgtcg gtctcgtcga cgtagaccgc
     1321 gtagacgtgc gggtcgtctc ccggcgcctg ccacatcgac catttgccgc tcgccggctc
     1381 gtagcggctc aagtttcccg agttccactc cgccacccag atatggccct tcgagtccga
     1441 ccagacgcgc cgcgcgccct ggcgccgcgt cggcggctcg atcaccgtcg tcgcgccgtt
     1501 tattgaattg atttttccca ggaaagaccc cgcaagagac gcgtagtaca ccgtgccatc
     1561 gggcgtggca tggatgccgt acgggccgcg gcccttgggc gcgtcgaaca gcttcatgtc
     1621 gccgctcttc ggatcgaggc tgccgtagat gccgctttga ccggtaaacc agtggatgcc
     1681 ctgcttgtcg aagatcgcgg tattgaggtt ggtgtagccg ctgccctcgg gcagcttcca
     1741 gcgcttcacc tgccgcgtct tcggatcgac gcgcacgatc gcgttctggc cgccgtcggt
     1801 gagccagggg gcgccgtcct cgccggcgat cacgccgtgc ggcgaggatc ccgagccgag
     1861 cgcgacgaac tccaccttgc ccgacttcgg gtcgaggatg ccgaggtggc cgctgccctg
     1921 ggcggagaac cacaccggac cgccgggcgc ggcgtccgcc cagacgtcgt gcacgcgatg
     1981 gccggacggg atcgggtatt cctggacgct gacctgcgcg cccgcggacg ccgtgaaaag
     2041 ccacagcaga agaatcctct tcatgtcagc cgcccggcag gctcttggcg attgccgcga
     2101 tcagaaccag catgcgtttc atggcgtctc ctcttcagga tttggcgagc tgcttcttcg
     2161 gatccacgaa cggcatcgcc accaccgtgg ccgcgcgcac gccgtcggct gtttcgacct
     2221 ggagattctt ctcggaaacc cgatcgatgg gaagcatgag gtagccgata tttttcctca
     2281 ggcgcgggga atagacggcc gaggtgacat ggccgccgcc gcgcaccagc cagcgcgtca
     2341 tgttgaggtc cagcctgccg ccctcgattt cgacaccgac cagcttgcgc ttgggttcgc
     2401 tcttaaagag tttcttcaag gcatcc
//
LOCUS       DASPXW010000272         4710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1342063, whole genome shotgun sequence.
ACCESSION   DASPXW010000272 DASPXW010000000
VERSION     DASPXW010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1342063"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..163
                     /locus_tag="VEL04_06225"
     CDS             2..163
                     /locus_tag="VEL04_06225"
                     /inference="COORDINATES: protein motif:HMM:NF024892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HYT47999.1"
                     /translation="MVGKFDFTEGALPFVKLNVKMGETSDVRVVAEAGGRYYVATREI
                     KVTIGGCGG"
     gene            189..488
                     /gene="soxZ"
                     /locus_tag="VEL04_06230"
     CDS             189..488
                     /gene="soxZ"
                     /locus_tag="VEL04_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HYT48000.1"
                     /translation="MKMRATLGNGYTDLRVLMSHPMETGQRKDPDGKLVPMHFIQSVV
                     VKVNGRTVVDGQISQGVSRNPVFSFRIKGGAKGDKIEVSWVDSQGESNKTEGAVA"
     gene            539..1285
                     /gene="soxA"
                     /locus_tag="VEL04_06235"
     CDS             539..1285
                     /gene="soxA"
                     /locus_tag="VEL04_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HYT48001.1"
                     /translation="MLAQDTKKEIQRYQQMIAEGSPVELFELEGETLWKKPQGPSNLS
                     LERCDLGLGPGMLKGAYARLPRYFKDADQVMDLESRLLYCMVTLQGRKREEATRRVFG
                     NDDRPSEMELLSAYIAGRSRGAKISPGLSHPREKQAYELGRALFFHRAGAWDFSCASC
                     HGEEGKRIRMQDLPVLYKPQHARPVVATWPAYRVSNSTFKTMQWRLNDCYRQMRMPEP
                     AFASETTVALTLFLTATGQGEPYRGPGTKR"
     gene            1282..1875
                     /gene="soxX"
                     /locus_tag="VEL04_06240"
     CDS             1282..1875
                     /gene="soxX"
                     /locus_tag="VEL04_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HYT48002.1"
                     /translation="MKLRAFFALAALASIAWAEPTPQDVEKVLLRDFHPRGQATMDRV
                     PQDSLQRLCTESADKPSAEVAKALEADQMKTIAFPGGDLMGDWQRGEKIAQGGRGLTW
                     SDRPGAPGDGSCYNCHQLAPAELSYGTIGPSLRGFGKLRGAGPEMQRYVYGKIYNAKA
                     YSLCSQMPRLGYSGTLTAEQIKDLVALLLDPASPVNR"
     gene            1876..3555
                     /gene="soxB"
                     /locus_tag="VEL04_06245"
     CDS             1876..3555
                     /gene="soxB"
                     /locus_tag="VEL04_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfohydrolase SoxB"
                     /protein_id="HYT48003.1"
                     /translation="MQRREFLQLLALAAAAGASLRPGASAAQAADELYEVPAFGNVSL
                     LHLTDAHGQLLPVYFREPGVSLGNGKPPHLVGEAFLKQYRIPGNSPAAYAFTYLDFAA
                     AARRYGKLGGFAHLATLVKRLRASRPHALLLDGGDSWQGSATALWTAGRDMIGAQKLL
                     GVDLMTAHWEFTYGAARVKRAVEAELAPIGFLAHNVKTVDFEDAVFEPYALRSVNGVP
                     LAVIGQAFPYTPIAHPRHLFPEWSFGIQEARLQGLVEEVRRKGAKLVALLSHNGMDVD
                     LKLASRVRGLDAILGGHTHDAVPAPVLVGRTLVTNAGSNGKFLAVLDFDVGSAGVKQY
                     RYRLLPVFSKLLPQDPEMTAYIAAARRPFEPKLRENLAVTEGLLYRRGNFNGTFDQLI
                     LDALLADQGAEIAFSPGFRWGTTLLPGDAITFEHVMGQTAITYPFVTLSELTGAGIKA
                     MLEDIADNLFNPDPYLQQGGDMVRVGGMSYALAPGAGIGARISDMRIANQPLQGGKRY
                     KVTGWAPVAEGAGGEAVWDVVARYLRAKRTIGPLDASRPRLIGVARNPGLA"
     gene            3568..4557
                     /gene="galE"
                     /locus_tag="VEL04_06250"
     CDS             3568..4557
                     /gene="galE"
                     /locus_tag="VEL04_06250"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HYT48004.1"
                     /translation="MRKVLVTGGAGYIGSHMVKLLLERGAEVTVLDDLSTGHRDAVVG
                     GHFVEGGLADPGRVAALLVDRGIEAVVHFAARSLVAESVADPLTYYSTNVGGSIVLLR
                     AMRDAAVRDIVFSSTAAVYGNPQRLPIDEAHPTQPLNPYGSSKLAVEQLLQDSSAAHG
                     LRAASLRYFNAAGADAQGALGERHAPETHLVPLVLQAAAGRRRAISVYGRDYPTRDGT
                     CVRDYIHVSDLCMAHLLALEWLADGGRYDCFNLGNGEGNTVLEVIDAARRVSGRDFEV
                     NFAARRAGDPAALVADATKAWRVLDWEPRYSDLETIMRHAWNFEQRRAPNRSA"
BASE COUNT          799 a         1559 c         1618 g          734 t
ORIGIN      
        1 gctggtgggc aagttcgact tcaccgaagg cgccctgccc ttcgtcaagc tcaacgtgaa
       61 gatgggcgag acctccgatg tgcgcgtagt ggccgaggcg ggcggcaggt actacgtggc
      121 gaccagggaa atcaaggtca ccatcggcgg ctgcggcgga tagggagacg acgatggcgg
      181 caggaccgat gaaaatgcgc gcgaccctcg gcaacggcta caccgacttg cgcgtgctga
      241 tgagccaccc gatggagacg gggcagcgca aggacccgga cggcaagctg gtaccgatgc
      301 atttcatcca gagcgtggtg gtgaaagtga acggcaggac cgtggtcgac ggccagatca
      361 gccaaggggt gtcgcgcaac cccgtgttct ctttccgcat caagggcggc gccaaggggg
      421 acaagatcga ggtgagctgg gtcgacagcc agggcgagag caacaagacc gaaggcgcgg
      481 tggcgtgatc cggaggctcg tatgcctctt tctcctcggg ggattttcgt gtacccccgt
      541 gctggcccag gacaccaaga aggaaatcca gcgctaccag cagatgatcg ccgagggcag
      601 cccggtcgag ctgttcgagc tcgagggcga gaccctgtgg aaaaagcccc aggggccgag
      661 caacctgtcg ctcgagagat gcgacctcgg gctggggccg ggcatgctga aaggcgccta
      721 tgcgcggttg ccgcgctact tcaaggacgc cgaccaggtg atggacctcg agtcgcggct
      781 tctgtattgc atggtcacgc tgcagggccg caagcgcgag gaggcgacgc ggcgcgtgtt
      841 cggcaatgac gaccggcctt ccgagatgga gctgctctcg gcttacatcg cggggcggtc
      901 gcgcggcgcg aagatctcgc ccggactgag ccatcccagg gaaaagcagg cttatgagct
      961 tggcagggcg ctgtttttcc atcgcgccgg cgcctgggat ttctcgtgcg cgagctgcca
     1021 cggcgaggaa ggcaagcgca tccggatgca ggacctgcca gtgctctaca agccgcagca
     1081 cgctcgcccg gtggttgcca catggcccgc ctaccgggtc tcgaacagca cattcaagac
     1141 gatgcagtgg cggctgaacg actgctaccg gcagatgcgc atgcccgagc cggccttcgc
     1201 ctcggagacg acagtggcgc tgacgctttt tctcaccgcg accggccaag gcgaacccta
     1261 ccgcggcccg ggaaccaagc gatgaagctc agagctttct tcgccctggc agcgcttgcc
     1321 tccatcgcat gggcggagcc gaccccgcag gatgtcgaga aggtgctgct gcgcgacttc
     1381 catccgcgcg gccaggcgac aatggatcgc gtcccgcagg acagtttgca gcggctttgc
     1441 accgagagcg cggacaagcc ctccgccgag gtcgcgaaag cgctcgaggc cgaccagatg
     1501 aagaccattg cgtttcccgg cggcgacctg atgggcgact ggcagcgcgg agaaaagatc
     1561 gcccagggcg ggcgcggcct cacctggagc gaccggcccg gagcgccggg ggacggcagc
     1621 tgctacaact gccaccagct cgcgcccgcg gagctgtcct acggcaccat cggcccgagc
     1681 ctgcgaggct tcggcaagct gcgcggcgca ggccccgaga tgcagcgcta cgtctacggc
     1741 aagatctaca acgccaaggc ctatagcctg tgctcgcaga tgccacggct cggctactcg
     1801 ggcacgctca ctgccgagca gatcaaggat ctggttgcgc tgctgctcga cccggcttct
     1861 ccggtgaacc ggtagttgca gcgacgcgaa tttctccagc tgctggcgct cgcggccgcc
     1921 gcgggcgcga gcctgcggcc cggcgcgagc gcggcccagg ccgccgacga gctctacgag
     1981 gtgccggcgt tcggcaacgt ttcgctgctg cacctcaccg acgcgcacgg ccagctgctg
     2041 ccggtttatt tcagggagcc gggcgtcagt ctcggtaacg gaaaaccgcc gcacctcgtc
     2101 ggcgaagcgt ttctcaagca gtaccgaatt cctggaaaca gcccggcggc ctatgccttc
     2161 acatatcttg atttcgctgc cgccgcgcgg cgctacggca agctcggcgg cttcgctcat
     2221 ctcgcaacgc tggtgaagcg cctgcgcgcg tcgcggccgc atgcgctgct gctggacggc
     2281 ggcgacagct ggcagggctc ggcgaccgcg ctctggaccg cggggcggga catgatcggc
     2341 gcgcagaagc tgctcggcgt cgacctgatg accgcgcact gggaattcac ctacggcgcg
     2401 gcgcgcgtca agcgggccgt cgaggccgag ctcgcgccga tcggtttcct cgcgcacaac
     2461 gtgaagaccg tcgacttcga ggacgcggtg ttcgagccgt acgcgctgcg cagcgtgaac
     2521 ggcgtgccgc tcgcggtgat cggtcaggcc tttccctata cgccgatcgc gcatccgcgc
     2581 cacctgtttc ccgaatggag cttcggcatc caggaagcgc gcctgcaggg cctggtggaa
     2641 gaagtgcgcc gcaagggcgc caagctggtc gcgctgctgt cgcacaacgg catggacgtc
     2701 gacctcaagc ttgcctcccg cgtgcgcggc ctcgacgcga ttctcggcgg gcatacccac
     2761 gacgcggtgc ccgcgcccgt gctggtcggc aggacgctgg tgaccaacgc cggctcgaac
     2821 ggaaaatttc tcgcagtgct cgacttcgac gtcggcagcg ccggcgtgaa gcaataccgc
     2881 taccgcctgc tgccggtgtt ctcgaagctc ctgccgcagg acccggagat gacggcctat
     2941 atcgccgcag cgcgccggcc cttcgagccg aaattgcgcg aaaaccttgc cgtgaccgaa
     3001 gggctgctct accggcgcgg caatttcaac ggtaccttcg accagctcat cctcgacgcg
     3061 ctgctcgccg accagggcgc ggagatcgcg ttctcccccg gttttcgctg gggcacgacg
     3121 ctgctccccg gagacgccat aaccttcgag catgtgatgg gccagacggc catcacctat
     3181 cccttcgtga cgctctccga gctgaccggg gccgggatca aggcgatgct cgaggacatc
     3241 gctgacaacc tgttcaatcc ggatccctac ctgcagcagg gcggcgacat ggtgcgcgtc
     3301 ggcggcatga gctacgcgct ggcgccgggc gcaggcatcg gcgcgcgcat ctccgacatg
     3361 aggatcgcga accagccgct gcaggggggc aaacgctaca aggtgaccgg atgggcgccg
     3421 gtcgccgaag gcgcgggtgg cgaagcggtc tgggacgtcg tggcccgcta cctgcgggcg
     3481 aagaggacca tcgggccgct cgacgcaagc cgtccccgcc tcataggcgt cgctcgcaac
     3541 ccgggtctgg cgtgagattc gaatagcgtg cgcaaggtgc tcgtcactgg cggcgcgggc
     3601 tatatcggct cgcacatggt gaagctgctg ctcgaacgcg gcgccgaggt cacggtgctc
     3661 gacgacctct ccaccggcca tcgcgacgcc gtggtcggcg gtcatttcgt cgaaggcgga
     3721 ctcgccgacc cgggccgcgt cgcggcgctg ctcgtggacc ggggcatcga ggcggtggtg
     3781 catttcgcgg ctcgcagcct ggtggccgag tcggtcgccg acccgctcac gtattactcg
     3841 acaaacgtcg gcgggagcat cgtgctgttg cgcgcgatgc gcgacgccgc ggtgcgcgat
     3901 atcgtgtttt cgtcgacggc cgcggtctat ggaaacccgc agcggctgcc gatcgacgag
     3961 gcgcatccga cgcagccgct caacccctac ggcagctcga agcttgccgt cgagcagttg
     4021 ctccaggact ccagcgccgc ccacggcctg cgggctgcaa gcctgcgcta tttcaatgcc
     4081 gccggcgccg acgcgcaggg tgcgctcggt gagcggcacg ctcccgagac gcacctcgtg
     4141 ccgctcgtgc tgcaggcggc cgcgggccgg cggcgcgcga tctccgtcta cggcagggac
     4201 tatccgacgc gagacggcac ctgcgtgcgc gattacatcc atgtctcgga tctctgcatg
     4261 gcgcatctgc tggccctcga gtggctggcg gacggcggca gatatgattg cttcaatctg
     4321 gggaacgggg agggaaacac cgtgctggag gtcatcgacg ccgcgcgccg ggtgtccggc
     4381 cgggacttcg aggtgaattt cgcggcccgc cgcgccgggg acccggcggc gctggtggcc
     4441 gacgcgacca aggcctggcg cgtgctggac tgggaaccac gctacagcga cctcgagacc
     4501 atcatgcgcc acgcatggaa cttcgaacag cgccgcgcgc caaaccgctc tgcgtagcgc
     4561 tggtcagccc ttattttcgg cgcttcgggt gaggcaagcg atgaaatgca gccggccgcc
     4621 gaagcaccag tttgcccacg gcggatagag gccgaggtcc tcgtcccagg actggactcg
     4681 aaggcgcagc gcgtcaaacg ccgcgtgcca
//
LOCUS       DASPXW010000273         3353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_833029, whole genome shotgun sequence.
ACCESSION   DASPXW010000273 DASPXW010000000
VERSION     DASPXW010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833029"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3125
                     /locus_tag="VEL04_06255"
     CDS             <1..3125
                     /locus_tag="VEL04_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48005.1"
                     /translation="GVALAWALIVASAGAAVWAVVRTGREAAAPALGRLIESAAGGRA
                     GSIVGVVSPTAGKGAGSSAALLLAADTRAATVVSFAAPGVNGVLRRATQRRVAAGAGA
                     ALVGVLLFLAGSPASGRAAAFWHPVRTWRDARAPVRLVVDQRTVRRGGSVTATITVPG
                     AVRVTLWTRGPGEPWKPMLLSLDTNGRVVQHVGPIESDLYLRASSGGRRSGEIKVDVA
                     LPAFLADLGLTARFPSYLDRSDEPLLVGPDVIPLPAGTTVLTSGTASVPLSSAAWTLE
                     SRVEQLRIAGTRIDGRFTPAASGTWRLRALSADGSALEGAAPELHLLIVPDSAPVVTL
                     PVPGRDTTLPISLHQQLVADVRDDHGITGLSVVSWRVSRTGRVGEAVRESLDVSGVGD
                     RAIVQGRLDAEHRGLLPGDTLRLYVEAFDNAPTPHTGRSAEIALRLPSMEELRAEARE
                     AARAVASAADSVSAAQRELSDRTRDLAQERSRDADGRAAGRPDGRNQQSTMSFQATQR
                     AEEIARDQAAMSQRVQELAQAVEEIARAAKAAGVDDSAFQNRLREVQELLQRALTPEL
                     EQRLRELQEALARLDPEATRQALQRLAEAQQQLRRELERSQELFRRAALEGELASLAK
                     DAEDLQRRQTEWTKEEAQHPDSAAAAAERDLAAATDSLARGVERAGETVPLQRSQAAA
                     RRAQAAMQEAANAASQRDSRGARASGQEAADSLAQLPNELRQRRDSVTSAWRQETLDA
                     LNRALSETAALARRQQDVADALRNGEAGATTRARQAAVEEGTHAIEEEISAAAGRHAL
                     VSPQLEAALGYAQNQMKQAREQLEQGDPNPATAAPFAEQALDALNATAHALVRTAQRV
                     ADAQSGSGFQEAIEQLARMAQQQQGMNGDAQGLLPMMAPGGDAVMQRLRELAGRQRAL
                     AEQLERLQAGGDAGAAGALAQEARELARQLEAGRLDARTIARQERLYRKLLDAGRSLT
                     GEEPDEQKERTSRSATGDSVHIPAALLPGTTGAGPKIRYPTWDELTGLTPEQRRMVLE
                     YFRRLNEAKH"
     gene            3109..>3353
                     /locus_tag="VEL04_06260"
     CDS             3109..>3353
                     /locus_tag="VEL04_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48006.1"
                     /translation="MKRSIRLALGLIVVLPALARAQAISPGFELERAGRYADAASVYF
                     TTVRSDPANIPALLGLERTLFVLNRMPELLPLVQTARA"
BASE COUNT          510 a         1180 c         1231 g          432 t
ORIGIN      
        1 cgggcgttgc gctcgcctgg gccctcatcg tggcgagtgc cggagcagcg gtgtgggcgg
       61 tcgtgcgcac gggccgtgag gccgccgcgc cggcgttagg cagactgatc gagagtgcgg
      121 ccggcgggcg agccggaagc atcgtcggag tggtcagccc aaccgcgggc aagggcgccg
      181 gttcgagcgc ggcgctgttg ctcgccgccg atacgcgcgc cgcaacagtg gtgtcgttcg
      241 cggcgcctgg cgtcaacggc gtactgcgcc gcgcgaccca gcggcgcgtg gcggccggcg
      301 ccggcgcggc gctcgttggt gttctccttt ttctcgcggg ctcgcccgcc tcgggccgcg
      361 ccgccgcgtt ctggcatccg gtacggacct ggcgggacgc gcgcgcgccg gtgcgcctag
      421 tggtagacca gcgcaccgtg cggcgcggcg gcagcgtcac ggcgacgatc accgtgccgg
      481 gcgccgtgcg cgtgacgctc tggacgcgcg ggcccggtga gccgtggaaa ccgatgttgc
      541 tttcgctcga tacgaacggc cgcgtcgtcc agcatgtggg cccgatcgaa tctgacctgt
      601 acctccgcgc gtcgagcggc ggacgccgca gcggcgagat caaggtcgac gtcgcgctgc
      661 cggcgtttct tgccgatctc ggactcactg cccgctttcc ttcatatctc gatcgctccg
      721 acgagccgct gctggtgggc ccggacgtca ttccactgcc cgctggtacc accgtgctca
      781 cgagcgggac cgcgagcgta ccgctctcga gcgccgcgtg gacgctcgag tcgcgcgtcg
      841 agcagctgcg cattgcggga acgcgcatcg acggccgctt cacgcccgcc gcaagcggca
      901 cctggcggct gcgggccctg agcgccgatg gatcggcgct cgagggcgcc gcaccggaac
      961 tgcatctcct gatcgtccct gattcggcgc cggtcgtcac gttgccggtc cccgggcgtg
     1021 atacgacgct gccgatttcc ctgcatcagc agctcgtcgc cgatgtgcgg gacgatcacg
     1081 gtatcacggg gctctccgtc gtaagctggc gcgtgagccg gaccggccgc gtcggcgagg
     1141 cggtgcgaga gtcgctcgac gtgagcggcg tcggggatcg ggcgatcgtg caggggcggc
     1201 tggatgccga gcaccgtggt ctcttgcccg gcgacacgct gcggctctac gtcgaagcgt
     1261 tcgacaacgc tcccacgccg cacaccgggc gcagcgccga gatcgcattg cgactgccca
     1321 gcatggaaga gctgcgggcc gaagcgcgtg aggcggcgcg ggcagtcgcg agtgcggccg
     1381 attcggtgtc cgcggcgcag cgagagctca gcgaccggac gcgtgacttg gcacaggaac
     1441 gcagccgtga tgcggacggt cgcgcggccg gacggcccga cggccggaat caacagagca
     1501 ccatgtcatt ccaagccacg cagcgcgcag aagaaatcgc gcgcgatcag gcggcgatgt
     1561 cgcagcgcgt gcaggagctg gcgcaggcgg tcgaggagat cgcgcgcgcc gccaaagcgg
     1621 ccggcgtgga cgacagcgcg ttccagaacc gcttgcgcga ggtgcaggag ctgttgcagc
     1681 gggcgctcac gcccgagctc gagcagcggc tccgcgagct gcaggaagcg ctcgcacgcc
     1741 tcgacccgga ggcgaccagg caagcgctgc aacgcctcgc cgaagcgcag cagcagctgc
     1801 gccgcgagct ggagcgcagc caggagttgt tccgccgggc cgctctcgag ggcgagctcg
     1861 cgtcactcgc gaaggacgcc gaagacctgc agcgccgcca gacggaatgg acaaaagaag
     1921 aggcgcagca tcccgactcg gcggcggcgg ccgccgagcg cgatctggcc gcggccaccg
     1981 attcgctcgc ccgcggcgtc gagcgcgccg gggaaaccgt gcccctgcag cgttcgcagg
     2041 ccgccgcgcg ccgcgctcag gccgccatgc aggaagcggc gaacgcggcg agccagcgcg
     2101 actcccgcgg cgccagggcg tccggacagg aagccgccga ttcgctggcg cagctcccga
     2161 atgagctgcg gcaacggcgc gattcggtga cctccgcctg gagacaggag acgctcgacg
     2221 cgctcaatcg tgcgctctcc gaaacggccg cgctcgcacg ccgccaacag gacgtcgccg
     2281 acgcgctgcg caacggggaa gcgggcgcca cgacgcgcgc gcgccaagcc gccgtcgaag
     2341 aggggacaca tgcgatcgag gaggagatca gcgccgcggc cggacggcac gcgctggtat
     2401 cgccgcagct cgaggccgcg ctggggtacg cgcagaacca gatgaagcag gcacgcgagc
     2461 agctcgagca gggcgatccc aatcccgcta ctgccgcacc cttcgcggag caggcgctcg
     2521 atgcgctcaa cgcgaccgcg cacgcactgg tgcgcacggc gcaacgagtc gccgatgcgc
     2581 agtcgggatc cggcttccag gaggcgatcg agcagctcgc gcgcatggcg caacagcagc
     2641 aggggatgaa cggcgacgcg caggggctgc tgccgatgat ggcgccgggc ggcgatgcgg
     2701 tgatgcagcg cctgcgtgag ctcgccggcc ggcagcgcgc cctcgccgag cagctcgagc
     2761 ggctgcaggc aggaggcgat gccggcgccg cgggtgcgct cgcccaggaa gctcgcgagc
     2821 tggcccggca gctcgaagcg ggacggctcg acgcccgaac catcgcgcgg caggagcggc
     2881 tgtaccgcaa gctgctcgat gccggccgca gcctcaccgg cgaggagccg gatgaacaga
     2941 aggagcgcac cagccgctcc gccacgggcg acagcgtcca cattccggcc gcgctgctcc
     3001 cgggtacgac cggcgcaggt cccaaaatcc gctacccgac gtgggacgag ctcaccggtc
     3061 tcacgcccga gcagcggcgc atggtgctcg aatacttccg ccgcctgaat gaagcgaagc
     3121 attaggctcg cgctcggcct cattgttgtg ctgccggcgc tggctcgggc tcaagccatc
     3181 agccccggct ttgagctcga gcgcgccggt cgctacgccg acgccgcgtc cgtctacttc
     3241 acgacggtgc gcagcgatcc ggcgaatatc ccggcgctcc tcggtctcga gcgcacgctg
     3301 ttcgtgttga accgcatgcc cgagctgctg ccgctcgtgc agactgcacg cgc
//
LOCUS       DASPXW010000274         2735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_833330, whole genome shotgun sequence.
ACCESSION   DASPXW010000274 DASPXW010000000
VERSION     DASPXW010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833330"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="VEL04_06265"
     CDS             complement(<1..836)
                     /locus_tag="VEL04_06265"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYT48007.1"
                     /translation="MVVSFAPGGVHDTLARLLSPRLSEALGQPVTVENRGGAGGNIAA
                     DAVAKSSADGYTFLVASEALATNEFLYRSLPYDLHKDLVPVAKLADFPMALVAHPSLS
                     ATSVAELVALARARPGEIHYGSAGIGTAGHLAGELMASATGISIVHVPYKGGAPALQD
                     LMAGRIQLMFLSVTLSAPQVRQGRLRALAIAGERRAEKLAEVPSTAEAGFPQLETLLF
                     SSLFAPAGTPAAIVQRMSTEAGKALRAPDAAKLLAELGAVPAPSSPEEFARQLREIAA
                     RWG"
     gene            complement(920..2029)
                     /locus_tag="VEL04_06270"
     CDS             complement(920..2029)
                     /locus_tag="VEL04_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008346690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HYT48008.1"
                     /translation="MPHPELFTPIQLGGVTLPNRIVVSPMCQYSADDGCMNDWHFVHL
                     GHLAYSGAGLLVVEATHVSREGRITHGCTGLYDERNEASIKKVIDNCRRVRKSPIGIQ
                     IGHAGRKASSQVPWEGRQWLTPEQSPWQTVAPSAIAYGKGWHVPKELSVGEIQQLVHA
                     FVLSAERAKRIGFDVLELHSAHGYLLHQFQSPLSNRRKDSYGANRLKFPLEVAQAVRD
                     TWPKERALGARISANDWMEGGLGPDDALPYARELKRIGFDYVCVSSGAMVPQARIPVA
                     PGYQLPFAERVKKSTDIKVQTVGMIADPEQAESIIASGKADMVVMARAFLDNPRWVWH
                     AAERFGVKLDYPPQYERSRHDLWPGSELARPRATA"
     gene            complement(2016..>2735)
                     /locus_tag="VEL04_06275"
     CDS             complement(2016..>2735)
                     /locus_tag="VEL04_06275"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HYT48009.1"
                     /translation="ESYGAWKMGADAAIMPLISSANIACGFHAGDPATIRATVRLAVE
                     HGVAIGAHPSLPDLAGFGRRAMKISPQEMYDLVIYQAGAVEAFARAAGSRLHHVKCHG
                     ALYNMAANDEALSEAMVRAAKDLGGVMLYVLSKSKNYETAKSMGVPVAGEVFADRGYS
                     DDGSLAPRDRPGGMIEDAGQSVKQALAMIEEGYVTSLSGKRVPVSAETLCLHGDQPNA
                     VAFAKALRKAFSEKGVRVAAS"
BASE COUNT          422 a          958 c          911 g          444 t
ORIGIN      
        1 ccccagcgcg ccgcgatctc gcgcagctga cgcgcgaact cctccggtga ggatggcgcc
       61 gggacggcgc caagctcggc cagcagcttt gccgcatccg gggcgcgcaa ggccttgccc
      121 gcctcggtgc tcatgcgctg cacgatggcg gcgggcgtcc ccgccggcgc gaacaggctc
      181 gagaacagca gcgtctcgag ctgcgggaat cccgcctcgg cggtcgaggg cacctcggcc
      241 agcttttcgg cgcgccgctc gcccgcgatc gcaagcgccc tgagcctgcc ctggcgcacc
      301 tgcggcgcgg acagcgtcac cgagagaaac atcagctgga tgcggcccgc catcaggtcc
      361 tgcagcgcgg gcgcgcctcc cttgtagggc acgtgcacga tgctgatgcc ggtcgcgctc
      421 gccatcagct cgcccgcgag atgcccggcg gtgccgatgc ccgccgagcc gtagtggatc
      481 tcgcccggcc gggcacgcgc cagcgcgacc agctcggcga cgctcgtcgc ggagagcgac
      541 ggatgcgcca ccagcgccat cgggaaatcg gcgagcttcg ccaccggcac gagatccttg
      601 tgcaggtcgt agggcagcga gcggtagagg aattcgttgg tggcgagcgc ctcgctcgcc
      661 acgaggaacg tgtagccgtc ggctgatgac ttcgcgaccg catcggcggc gatattgccg
      721 cccgcgccgc cgcggttctc caccgtgacc ggctggccga gcgcctcgga gagcctgggc
      781 gagagcaggc gcgcgagcgt atcgtgcacg ccgccgggcg cgaacgacac caccatccgg
      841 atcggctttt caggccaggg ctggccaaac gcggggcctg ccgccagaac ggcgcaggcc
      901 agcgcggcag aaaaatgctt caagcggtcg cgcgcggccg cgccagctcg cttcccggcc
      961 acaggtcgtg ccggctgcgc tcatactgcg gcggatagtc gagcttgacg ccgaagcgct
     1021 cggcggcgtg ccagacccag cgcgggttgt ccagaaaagc gcgcgccatc accaccatgt
     1081 cggccttccc cgaagcgatg atcgactccg cctgctccgg atcggcgatc atgccgaccg
     1141 tctggacctt gatgtctgtt gatttcttta cccgctcggc aaacggcagc tggtagcccg
     1201 gcgccaccgg aatacgcgcc tgcggcacca tggcgccgct cgaaacgcag acgtagtcga
     1261 agccgatgcg tttcagctcg cgcgcgtagg gcagcgcgtc gtcgggtccg aggccgcctt
     1321 ccatccagtc gttcgcgctg atgcgcgcgc cgagcgcgcg ctccttcggc caggtgtcgc
     1381 gcacggcctg cgccacctcg agaggaaatt tgagcctgtt tgccccatag gaatcttttc
     1441 ttcgattcga gagcggcgac tggaactgat gcagcaggta gccgtgcgcc gagtgcagct
     1501 cgaggacgtc gaagccgatg cgttttgccc tctccgctga aagaacaaaa gcgtggacca
     1561 gctgctggat ctccccaacg gacaattcct tgggcacgtg ccagcccttg ccatacgcaa
     1621 tcgccgacgg cgccaccgtc tgccacggtg actgctccgg cgtgagccac tggcgccctt
     1681 cccacggcac ctgcgacgag gccttgcgcc cggcgtggcc gatctggatg ccgatgggag
     1741 atttgcgcac acggcggcag ttatcgatta ccttttttat ggaagcttcg ttgcgctcgt
     1801 cgtagaggcc ggtgcagccg tgcgtgatgc ggccctcgcg gctgacgtgc gtcgcctcca
     1861 ccaccagcag ccccgcgccc gagtaggcga gatggccgag atgcacgaag tgccagtcgt
     1921 tcatgcagcc gtcgtcggcg gagtactggc acatcggcga caccacgatg cggttgggca
     1981 gcgtcacgcc gcccagctgg atcggggtaa agagctcagg atgcggcaac gcggacgcct
     2041 ttttcggaaa atgcttttcg cagcgccttg gcgaacgcta ccgcgttcgg ctggtcgccg
     2101 tgcaggcaca gcgtctccgc ggacaccggc acgcgcttcc ccgagaggct ggtgacgtag
     2161 ccttcctcga tcatcgccag cgcctgcttc acggactggc cggcgtcctc gatcatgccc
     2221 ccgggccggt cgcgcggcgc gaggctgccg tcgtcggagt agccgcggtc ggcgaacacc
     2281 tcgcccgcga ccgggacgcc catgcttttc gctgtttcat agtttttact ttttgacaga
     2341 acataaagca tgaccccgcc gagatccttc gcggcgcgca ccatggcctc cgacaatgcc
     2401 tcgtcgttcg ccgccatgtt gtagagcgcg ccgtggcact tcacgtgatg cagccggctg
     2461 cccgcggcgc gcgcgaatgc ctcgaccgca cccgcctggt agatcaccag gtcgtacatc
     2521 tcctgcgggc tgattttcat cgcgcgcctg ccgaagccgg cgaggtcggg cagcgacgga
     2581 tgcgcgccga tcgccacgcc gtgctcgacg gcgagccgca ccgtggcgcg gatcgtcgcc
     2641 ggatcgccgg cgtggaagcc gcaggcgatg ttcgccgagc tgatgagcgg catgatggcg
     2701 gcgtcggcgc ccatcttcca cgcgccgtag ctctc
//
LOCUS       DASPXW010000275         3447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1466584, whole genome shotgun sequence.
ACCESSION   DASPXW010000275 DASPXW010000000
VERSION     DASPXW010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1466584"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VEL04_06280"
     CDS             <1..279
                     /locus_tag="VEL04_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYT48010.1"
                     /translation="TNADRVLPSAYETAVAKAACNQAGFEVANDAIQAMGALGYSSET
                     LVEYCMRRCRGWMIAGGSIEMLKNRIAEHVFDRRFDQRPPRAKPQAAE"
     gene            298..2532
                     /locus_tag="VEL04_06285"
     CDS             298..2532
                     /locus_tag="VEL04_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HYT48011.1"
                     /translation="MNRSPSPAELAQYLLAPRSVAIVGQSNDAGKTAGRPLKYLRQAG
                     YAGRLYPINPARDTVLGERAWPSLAALPEVPEHVYVVAPTEPSIEAVAEAGRLGVKVA
                     TLLADGFAEAGPQGDARVAKLRAICAETGIRVVGPSSLGVVDLRSKALITANAAFDET
                     ELPAGRIFAASHSGSMIGALLSRGKARGIGFAGLVSVGNEVDLSLGEICALTLDDPGI
                     DGYMLFLETMRNTAALRHFAMAASERGKPILAYKLGRSAAARELAVTHTGALAGEDDI
                     ADAFLAACGIARAETLEGLLEGLPLLQRMPAANATRRMAVGVVTTTGGGATTAIDPLA
                     MRGITIASPSADTLTRLKAAGIDIKPARLVDLTLTGTRYDVMKAALDILTTAPEFDLI
                     LCVVGSSARFYPELAVRPIVDSAGKPIAAYLVPDAPQALAMLSRAGVPNFRTPEACAD
                     AIAAALRRRKPVAPPPQRDLSTGVATGRATSFPPPLRGRDREGGPTDSESGTTPLPTP
                     PPQGGREQAEFAATAVPSSTREVSPRLIDELEAYELLGKRGLTCAPSLALDADLTTAP
                     ALPFAYPVVVKALSAQLAHKSDAGGVVLNVADGAALVAACQRIRANVAAHGHDIARVL
                     VQPMISGVGEVLIGYRVDPDVGPLLMVAMGGTFTEIYRDRSLRLAPVDLATAREMIAE
                     VRGLATLKGFRGKPAGDLDALAQAIVALSQLADDALVFEAEINPLIVKTEGVVAVDAV
                     VWLA"
     gene            2669..3004
                     /locus_tag="VEL04_06290"
     CDS             2669..3004
                     /locus_tag="VEL04_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48012.1"
                     /translation="MLYDPGLTAPARSASVPRIRSSKGDEAVMDPVLFVLVYFFAYTI
                     TFIVPVARIFKRVGLNPWLAILTVIPLVNLFGLWFFAFSPWPSDSAKDRQEEDWSEAD
                     KEKFRHLMR"
     gene            3143..>3447
                     /locus_tag="VEL04_06295"
     CDS             3143..>3447
                     /locus_tag="VEL04_06295"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HYT48013.1"
                     /translation="MSHAEALNARHPAAGATEAITGFVSALRYSALSEEVRHYARRHL
                     FDTVGVMIAGAGGEVATRAEAVLASVRPAGSVPVPGRARRADLIDAAFLGGTAAHGI"
BASE COUNT          539 a         1194 c         1120 g          594 t
ORIGIN      
        1 accaatgcgg atcgtgtcct gccctcggcc tacgagaccg cggtcgcgaa ggcggcctgc
       61 aaccaggcgg gcttcgaggt cgccaacgac gcaattcagg cgatgggcgc gctcggctat
      121 tcgagcgaga cgctggtgga atattgcatg cgtcgctgcc gcggctggat gatcgcaggc
      181 ggctcgatcg agatgctcaa gaaccgcatc gccgagcatg tgttcgaccg ccgcttcgac
      241 cagcgcccgc cgcgcgccaa gccccaggcg gcggagtgac cctctgaagt agccagcatg
      301 aatcgatccc cctcccctgc cgagctggcg caatatctgc tcgcaccgcg ctcggttgcg
      361 atcgtggggc agtcgaacga cgccggcaag accgccggcc ggccgctgaa atatctgcgc
      421 caggccggtt atgccggccg cctctatccg atcaacccgg cacgcgacac cgtgctgggc
      481 gagcgcgcct ggccgtcact cgccgcgctg ccggaagtgc ccgagcacgt ctatgtggtg
      541 gcgccgaccg agccttcgat cgaagcggtc gcggaggcgg ggcgccttgg cgtcaaggtc
      601 gccacgctgt tggctgatgg tttcgccgag gccggcccgc aaggcgacgc ccgcgtcgca
      661 aagctgcgcg cgatctgcgc cgagacgggc attcgcgtgg tcggcccctc gagcctcggc
      721 gtggtcgatc tgcgctcgaa ggcgctgatc actgccaacg cagccttcga cgagaccgag
      781 cttcccgccg gtcggatctt tgctgcgtcc cattccggca gcatgatcgg ggcgctgctt
      841 tcacgcggca aagcgcgcgg catcggcttt gcgggtcttg tctcggtcgg caacgaggtc
      901 gatttgtcgc tgggcgagat ctgcgcgctc acgctcgatg atcccggtat cgatggctac
      961 atgctgtttc tcgaaaccat gcgcaacacc gcggcgctgc ggcactttgc aatggcggcg
     1021 tcggagcgtg gcaagccgat cctcgcctac aagctcggcc gctcggcggc ggcgcgcgag
     1081 ctcgctgtca cccacaccgg cgcgctcgcc ggcgaggacg atatcgcgga tgcgttcctc
     1141 gccgcctgcg gcatcgcgcg cgccgaaacg ctcgagggcc tgcttgaagg cctgcccctg
     1201 ttgcaacgca tgccggccgc aaatgcgacg cgccgcatgg cggtcggtgt ggtcacgacc
     1261 accggcggcg gcgccaccac cgcgatcgat ccactcgcga tgcgcggcat cacgatcgca
     1321 tcgccctccg ccgacacgct cacgcgcctc aaggctgccg gcatcgacat caagccggcg
     1381 cgcctggtcg atctcacgct caccggcacg cgctacgacg tgatgaaggc cgcgctcgac
     1441 attctcacca ccgcgcccga gttcgatctc attctgtgcg tggtcggctc ctcggcgcgc
     1501 ttctatccgg agctcgcggt gcggccgatc gtcgacagcg ctggcaagcc catcgcggcc
     1561 tatttggtgc ccgacgcgcc gcaggcgctc gcgatgctca gtcgcgccgg tgtgccgaac
     1621 tttcgcacac cggaagcctg cgcggacgcg atcgcggcag cgctgcggcg gcgcaaacca
     1681 gtggcgcctc cgcctcaacg cgatctctcg accggcgttg cgacgggccg cgccacatcg
     1741 ttccctcccc ccctgcgggg gagggacagg gaggggggtc caacagattc tgagtccgga
     1801 accacccccc tccccacccc tcccccacag ggggggaggg agcaggccga gttcgccgca
     1861 accgcagtgc cctccagcac cagagaagta agcccgcgtc tcatcgacga acttgaagcc
     1921 tacgaactgc tcggcaaacg cggcctcacc tgcgcgccct cgctcgcgct cgatgccgat
     1981 ctcaccacgg ctcccgccct gccctttgcc tatccggtcg tcgtcaaggc gctctcggct
     2041 caactcgcgc acaagagcga tgccggcggc gtcgtgctca acgtcgcgga tggcgccgcg
     2101 cttgtcgccg cctgtcagcg cattcgcgcg aatgtcgcgg cgcatggcca cgacatcgcg
     2161 cgcgtgctgg tgcaaccgat gatcagcggc gtcggcgaag tgctgatcgg ctatcgggtc
     2221 gatcccgatg tggggccgct cctcatggtg gcaatgggcg gcaccttcac cgagatctat
     2281 cgcgaccgca gtctgcggct tgcaccggtt gatctcgcga ctgcgcgcga gatgatcgct
     2341 gaggtgcgcg gccttgcgac gctcaagggc tttcgtggca agcccgcggg cgatctcgat
     2401 gcgctcgcgc aggcgatcgt ggcgctgtcg cagcttgcgg acgatgcgtt ggtatttgag
     2461 gcagagatca atccgctgat cgtcaagacg gaaggtgtcg tggccgtcga tgcggtggtg
     2521 tggctcgcct gaacgcactc gacgggtgta aagtcattct ccgccaagaa ggctcacgct
     2581 ggcccccctc aagggaggac gaccgcgcca ccgctgctca cctgccgcga cgtgctcgag
     2641 ccccgatgcc aaggctcccg cataacgagt gctctacgat ccagggttga cagcgcctgc
     2701 ccgttctgct tctgttccgc gcatccgcag cagcaagggg gatgaagcgg tcatggaccc
     2761 cgtattgttc gttcttgtct acttcttcgc ctacaccatc accttcatcg tgccggttgc
     2821 acgaatattc aaacgggtgg gcctcaatcc atggcttgcc atcctgaccg tcatcccgct
     2881 cgttaacttg ttcggcctct ggttctttgc cttcagcccc tggccctcgg attcggcaaa
     2941 ggacagacag gaagaggatt ggtcggaagc ggacaaggaa aaattccggc acttgatgcg
     3001 gtaacagtaa gcacacgcca aaggcgtcag cgttctcgcg ccgcttcgac gatcgccgca
     3061 ttcctcagaa cggcggaagg cgatgaggga cagcgcgtct ccgctgcgct aggttcaggc
     3121 cgctttttgc aagaggtgcc cgatgtccca tgccgaagcg ctcaatgccc gccaccccgc
     3181 cgccggggct accgaggcca tcacgggctt tgtcagcgcg ttgcgctata gcgcgttaag
     3241 cgaggaggtg cgtcactatg cgcgccggca tttgttcgac acggttggcg tgatgatcgc
     3301 gggcgccggt ggcgaggtcg cgacccgcgc cgaggcggtg ctcgcgagcg tgcgcccggc
     3361 cggcagcgtg ccggtgccgg gacgtgcgcg gcgcgccgac ctcatcgacg cggcgttcct
     3421 gggcggcacc gccgcgcatg gcatcga
//
LOCUS       DASPXW010000276         4821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1089941, whole genome shotgun sequence.
ACCESSION   DASPXW010000276 DASPXW010000000
VERSION     DASPXW010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4821
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089941"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VEL04_06300"
     CDS             complement(<1..185)
                     /locus_tag="VEL04_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HYT48014.1"
                     /translation="MRLSINGKTHDVDVEPSTPLLWALREQVGLTGTKYGCGMAQCGA
                     CTVHVDGVAVRSCSFPV"
     gene            289..756
                     /locus_tag="VEL04_06305"
     CDS             289..756
                     /locus_tag="VEL04_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HYT48015.1"
                     /translation="MSELYLEDFAVGQTYGSGRLRVDEEKIKAFAAEFDPQPFHLDAA
                     AARDSIFRGLAASGWHTAALTMRLLVGSELKPAGGIVGAGFDEFRWPRPVRPGDELRV
                     ESEVLEVRASKSRPDQGLIKVRTTTLNQNGEAVQISIGNLLVPRRPATGAAKS"
     gene            complement(766..1200)
                     /locus_tag="VEL04_06310"
     CDS             complement(766..1200)
                     /locus_tag="VEL04_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48016.1"
                     /translation="MKAALVSLCLALAACTEMQTGKPVREGVLPDGATYQDFSHQPHG
                     YLRVTTTTDGRKEVHDLHTEENFRHLQPGMTPGEVEDVVGVSSVGKAAYANGTTSWTY
                     RYRDIGVAKLLHVMFGPDGRVLRYETEWDPNVYSKKTGGGGK"
     gene            complement(1197..1667)
                     /gene="greA"
                     /locus_tag="VEL04_06315"
     CDS             complement(1197..1667)
                     /gene="greA"
                     /locus_tag="VEL04_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HYT48017.1"
                     /translation="MKTPMTKRGAEMLREELHRLKSVERPANSAAIGEARSHGDLSEN
                     AEYHAARERAGFIEGRISEIEAKLANAQVIDPRLVDAEGRCVFGATVDVEDEAGEKST
                     WQIVGEDEADIKQGRISVSSPIARALISKESGERVEVHTPGGVKRYEIIDVRYE"
     gene            complement(1664..>4821)
                     /gene="carB"
                     /locus_tag="VEL04_06320"
     CDS             complement(1664..>4821)
                     /gene="carB"
                     /locus_tag="VEL04_06320"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HYT48018.1"
                     /translation="FDYSGAQACKALREEGYKVILVNSNPATIMTDPEMADVTYIEPI
                     TWAMIAKIIERERPDALLPTMGGQTALNCSLDLAREGVLNKFDVELIGASKKAIDKAE
                     DREKFKLAMRRIGLACPRSSLAHSMEEALQVQAGIGFPVVIRPSFTLGGTGGGIAYNR
                     EEFVAICERGLDASPTRELLIEESVLGWKEFEMEVVRDRKDNCIIVCSIENLDPMGIH
                     TGDSITVAPAQTLTDKEYQIMRDASIQVLREIGVDTGGSNVQFAIHPGDGRMLVIEMN
                     PRVSRSSALASKATGFPIAKVAAKLAVGYTLDEIANEVTGGATPASFEPTIDYVVTKV
                     PRFAFEKFPLANDRLTTQMKSVGEVMAIGRTFQESLQKALRGLEIGVDGLNQRTRDRE
                     TLEKELGEPGPERIWYVGDAFENGFTLEEVHRLTHIDPWFLDQIREIVELEMQLDDAK
                     LEEIDEESLRKLKRKGFSDRRLAYLFNTHEKAVREKRHKLGIRPVFKRVDTCAAEFAA
                     RTAYLYSTYEEECEAAPTAKQKIVVLGGGPNRIGQGIEFDYCCVHAALALRDDGFETI
                     MVNCNPETVSTDYDTSDRLYFEPLTLEDVLEIVDAEKPWGVIVQYGGQTPLKLARDLA
                     AAGVPIIGTSADSIDVAEDRERFQQLLTRLKLRQPPNRTARSAEEAVRLAEEIGYPVV
                     VRPSYVLGGRAMEIVHDKINLLQYTERAVRVSNDSPVLLDRYLADAIEIDVDCVCDGK
                     DVMIGGIMEHVEQAGVHSGDSACCLPPHSLASHVERELRRQTELLARSLKVVGLMNVQ
                     YAIQRDAVYVLEVNPRASRTVPYVSKATGLPLAMIGARCMAGRTLKEQRIEEVTPRYF
                     SVKEAVFPFAKFPGVDTILGPEMKSTGEVMGVGETFGEAFVKSQIAAGVRLPLGGRAF
                     ISVRDTDKLEAVKIARDLLELGFTLLATRGTAAVLKSHGLPVTQVNKVAEGRPHIVDM
                     IKNGEVSFIVNTTEATRTAASDSRSIRTTALTRRVTYYTTVAGARAACAGMKHLAELE
                     PYRLQDLHARLRRAEPEQVAS"
BASE COUNT          750 a         1621 c         1574 g          876 t
ORIGIN      
        1 ctcaccggga acgagcacga gcgcacggcg acgccgtcga catgcaccgt gcaggcgcca
       61 cattgggcca tgccgcagcc gtacttggtg ccggtcagac cgacctgttc cctgagcgcc
      121 caaagcagcg gcgtgctcgg ctcgacatcg acatcgtgag tcttgccatt gatggatagg
      181 cgcatctgct gtctcctccc ggtcgtctcg atgcctgcgg agccgcaagc gacaagctac
      241 tgtagacccg gcgtccacta gaatcaaccg ggggatcgaa cgcacggcat gagcgagctt
      301 tatctggagg atttcgccgt cgggcagaca tacggctccg ggcggctgcg ggtcgacgag
      361 gagaagatca aggcgttcgc cgccgaattc gacccgcagc ctttccatct ggacgccgcc
      421 gccgcgcggg acagcatctt tcgcgggctg gcggcaagcg gctggcacac cgccgccctc
      481 actatgcggc tgctggtcgg cagcgagttg aagcccgccg gcggcatcgt cggcgccggc
      541 ttcgacgagt tccgctggcc gcggccggtg cgcccaggcg atgagctgcg cgtcgagagc
      601 gaggtgctcg aagtgcgggc gtcgaagtcg cgccccgacc agggcctgat caaggtgcgc
      661 accaccacct tgaaccagaa cggtgaagcg gtgcagatct ccatcggcaa cctgctggtg
      721 ccgcgacgcc cagcgaccgg agccgccaag agttgagcgc cggccctact tgccgccgcc
      781 gccggttttc ttcgagtaga cgttcgggtc ccactcggtc tcgtagcgca acacgcggcc
      841 atcaggcccg aacatcacgt gcagcagctt ggcgacaccg atgtcgcggt agcgataggt
      901 ccaggacgtg gtgccgttcg cgtaggccgc ttttccgacg gagctcaccc caacaacgtc
      961 ttcgacctcg cccggcgtca tgccgggctg cagatgacgg aaattctcct cggtgtgcag
     1021 gtcgtgcact tctttgcggc cgtcggtcgt ggtggtgacg cgcagatagc cgtggggctg
     1081 gtgcgaaaaa tcctggtagg tcgcgccgtc gggcagcaca ccctctctga ccggcttgcc
     1141 ggtctgcatc tcggtgcaag ccgcaagggc gaggcaaagc gaaacgagcg ccgccttcat
     1201 tcgtagcgca cgtcgatgat ctcgtagcgc ttcacgccgc cgggggtgtg cacttcgacc
     1261 cgctcgccgg actccttgct gatcagcgcc cgggcgatcg gcgagctcac cgagatgcgg
     1321 ccctgcttga tgtcggcctc gtcctcgccg acgatctgcc aggtggattt ctcgccggcc
     1381 tcgtcctcga cgtccacggt ggcgccgaac acgcagcgtc cctcggcgtc gaccagcctg
     1441 ggatcgatca cttgcgcgtt ggcgagcttc gcctcgatct cggagatgcg gccctcgatg
     1501 aagccggcgc gctcgcgcgc cgcgtggtac tcggcgttct ccgacagatc gccgtgcgag
     1561 cgcgcctcac cgatcgccgc cgagttggcg ggccgctcga ccgacttcag tcgatgcagc
     1621 tcctcgcgca gcatctcggc gccgcgtttc gtcatggggg ttttcatgaa gccacctgtt
     1681 ccggctcggc gcggcgcagc ctcgcatgca ggtcctgcag gcggtagggc tcgagctcgg
     1741 cgaggtgctt cattcccgcg caggccgccc tggcgccggc cacggtagtg taataggtga
     1801 cgcggcgggt gagcgccgtg gtgcggatgg agcgcgagtc ggaggcagcg gtgcgcgtcg
     1861 cctcggtggt attgacgatg aagctcacct cgccgttctt gatcatgtcg acgatgtgcg
     1921 ggcggccctc ggcgaccttg ttcacctgcg tcaccgggag gccgtgcgac ttcagcaccg
     1981 ccgcagtgcc gcgcgtagcg agcagggtga agcccagctc gagcagatcg cgcgcgattt
     2041 tcaccgcctc gagcttgtcg gtgtcgcgca cgctgatgaa cgcgcgcccg cccagcggca
     2101 agcgcacgcc ggccgcgatc tgtgatttga cgaaggcctc gccgaaggtc tcgccgacgc
     2161 ccatcacctc cccggtcgat ttcatctcgg gcccgagaat ggtgtcgacg cccgggaact
     2221 tggcgaacgg gaacaccgcc tcctttaccg agaagtagcg cggagtcacc tcctcgatgc
     2281 gctgctcctt cagcgtgcgg ccggccatgc agcgcgcgcc gatcatcgcg agcggcagcc
     2341 cggtcgcctt ggagacgtac ggcacggtgc gcgaggcgcg ggggttgact tccagcacgt
     2401 agaccgcgtc gcgctggatg gcgtactgca cgttcatcag gcccaccacc ttgagagacc
     2461 gggcgaggag ctcagtctgg cgcctgagct cccgctcaac gtggctcgcg agcgaatgcg
     2521 gcggcaggca gcaggccgag tcgcccgaat gcacgcccgc ctgctcgacg tgctccatga
     2581 tgccgccgat catcacgtcc tttccgtcgc acacacagtc gacgtcgatc tcgatcgcgt
     2641 cggccaggta gcggtcgagc agcaccgggg aatcattgga gacgcgcacc gctctttcgg
     2701 tgtactgcag aagattgatc ttgtcgtgga cgatctccat ggcgcggccg ccgagcacgt
     2761 agctcgggcg caccaccacc gggtaaccta tctcctcggc gagcctcacc gcttcctcgg
     2821 cggaacgcgc cgtccggttg ggcggctggc gcagcttcag ccgggtgagc agctgctgga
     2881 agcgctcgcg gtcctccgcg acgtcgatcg agtcggcgct ggtgccaatg atcggcacgc
     2941 cggccgccgc aaggtcgcgc gcgagcttga gcggcgtctg cccgccgtac tgcacgatca
     3001 cgccccaggg cttctcggcg tcgacgatct cgagcacgtc ctcgagcgtg agcggctcga
     3061 aatacagccg gtcggaggtg tcgtagtcgg tcgagacggt ctcggggttg cagttcacca
     3121 tgatggtctc gaagccgtca tcgcgcagcg ccagcgcggc gtgcacgcag cagtagtcga
     3181 actcgatgcc ctggccgatg cggttcggcc cgccgcccag caccacgatc ttctgcttcg
     3241 ccgtcggtgc agcctcgcac tcctcctcgt aggtcgagta caggtaggcg gtacgggccg
     3301 cgaactcggc ggcgcaggtg tcgacgcgct tgaacaccgg gcggatgccg agtttgtgtc
     3361 gcttttcgcg taccgccttc tcgtgcgtat tgaacaggta ggcgaggcgc cggtccgaga
     3421 agcctttgcg cttcagtttt ctcagagact cttcatcgat ttcttccagc ttcgcgtcat
     3481 cgagttgcat ttcgagctcg acgatctccc tgatctggtc gaggaaccac gggtcgatgt
     3541 gcgtcaggcg atgcacttct tccagcgtga agccgttctc gaaggcatcg ccgacgtacc
     3601 agatgcgctc ggggcccggc tcgccgagct ccttctccag cgtctcgcgg tcgcgcgtgc
     3661 gctggttgag cccgtcgacg ccgatctcca ggccgcgcag cgccttctgc agcgattcct
     3721 ggaaggtgcg gccgatcgcc atcacctcgc cgaccgactt catctgggtg gtgagccggt
     3781 cgttggcgag cggaaacttc tcgaaggcga agcgcggcac cttggtcacg acgtaatcga
     3841 tcgtcggttc gaacgaggcg ggcgtcgcgc cgccagtgac ttcgttcgca atctcgtcga
     3901 gggtgtaacc caccgcgagc ttcgccgcca ccttggcgat cgggaagccg gtcgccttgg
     3961 aggcgagcgc gctggagcgc gacacgcgcg ggttcatctc gatcaccagc atgcgcccat
     4021 cgccgggatg gatcgcgaac tgcacgttcg agccgcccgt gtcgacgccg atctcgcgca
     4081 gcacctggat cgaggcgtcg cgcatgatct ggtattcctt gtcggtgagc gtctgcgccg
     4141 gcgccacggt gatcgagtcg ccggtgtgga tgcccatcgg gtcgaggttc tcgatcgagc
     4201 agacgatgat gcagttgtcc ttgcggtcgc gcaccacctc catctcgaac tctttccagc
     4261 cgagcaccga ctcctcgatc agcagctcgc gcgtcggcga ggcatcgagc ccgcgctcgc
     4321 agatcgcgac gaattcctcg cggttgtagg cgatgcctcc cccggttcct cccaatgtga
     4381 aggaaggcct tataaccacg gggaaaccga tgcccgcctg cacctgcagc gcttcctcca
     4441 tcgagtgcgc aagcgaagag cgcgggcagg caaggccgat gcgccgcatg gcgagcttga
     4501 acttctcgcg gtcctccgcc ttgtcgatcg ccttcttcga cgcaccgatc agctcgacgt
     4561 cgaacttgtt gaggacgccc tcgcgcgcga gatcgagtga gcagttcaac gccgtctgcc
     4621 cgcccatcgt cggcagcagc gcatcgggac gctcgcgctc gatgatcttc gcgatcatcg
     4681 cccaggtgat cggctcgatg tacgtcacgt cggccatttc gggatcggtc atgatcgtcg
     4741 ccggattgga gttgaccagg atgaccttgt agccctcctc gcgcagcgcc ttgcacgcct
     4801 gcgcgcccga ataatcgaac t
//
LOCUS       DASPXW010000277         3519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1090298, whole genome shotgun sequence.
ACCESSION   DASPXW010000277 DASPXW010000000
VERSION     DASPXW010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090298"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="VEL04_06325"
     CDS             complement(<1..537)
                     /locus_tag="VEL04_06325"
                     /inference="COORDINATES: protein motif:HMM:NF017658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HYT48019.1"
                     /translation="MRTQPRAAVLAVSAALMPWVWMQPALADPAANQLPTGGQVAAGT
                     ANISTAGSKMQIDQATDKAILNWQTFSIGSSAWVNFSQPSASSVALNKVLGNNPSEIF
                     GRLSANGQVFLSNPSGVLFAPSASVDVGSLFATSLSITDQDFLAGRYNFYNSGSAGSV
                     VNQGTIITTNGYAALAGPQ"
     gene            complement(718..2418)
                     /locus_tag="VEL04_06330"
     CDS             complement(718..2418)
                     /locus_tag="VEL04_06330"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HYT48020.1"
                     /translation="MSLHRLVAFVVFSVASALVLAQQKPDAGQILEQTREPLRLPPPS
                     PDLRPKPPEPKPALGAQPQLKVKVSRFTFSGNTLIPEDELQEAVKEFVGKELNFEGLN
                     DAATEARAYYRSRGYFLAQAYLPQQAIRGGSVEITIIEGRWDVIELNTKPATQISTRL
                     LAGIINAHLQQSDIITETGLERPLLLINDLPDASVTTELRPGETVGAANLRVNVDPGA
                     GSFNGFVDIDNYGNRFTGEYRLGANLNLSNPAGYGDRATLRAFTTDEKMWYARLAYDL
                     PVGYYGTRVGVSYSKFDYALSKDFASLGANGEGEVKGLYAFHPIVRTRNTNLILNVAY
                     EEKQLIDRIENPASLKQSNIQTTKVAAVGDFRDAILGGGLNAYSLAYTFGNLGLSPVA
                     AESADLAATGRQTEGNFHKTNYDLRRQQRVSNDASILFSFSHQWASRNLASAEKMSLG
                     GPNGVRAYPVGEATADSGMIATVEPRYIVPGTKIFDGDMAFLAFFDVGWAKVNQIPLS
                     TDGENIRHISGVGLGTSLGKEGGFLVRASMAWKYTRQDPESDKARRVPRVWIQAIKWF
                     "
     gene            complement(2801..>3519)
                     /locus_tag="VEL04_06335"
     CDS             complement(2801..>3519)
                     /locus_tag="VEL04_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYT48021.1"
                     /translation="QVVVMNVLPGLVVALLIFAFLGTLQLSAKKSHGESQSLRAYLGG
                     LRSLFRNRALLLLCAGSSFRTMTQTALLTFLPVFLANSMGYSPAWIGACLFALQAAGF
                     AAAPVAGHLSDRMGRKHILMGSMATSAVVLALMAFAGGSPLFIGLVAVLGFFLYATRP
                     VIQAWLLETTPKNMGGSSIGVLFGAQAIGGSLGPLLGGMVADHYGLLATFYFLAGTIV
                     IANLFVIWVPKGEPAAVSGT"
BASE COUNT          645 a         1095 c         1138 g          641 t
ORIGIN      
        1 ttgcgggccg gcgagggccg catagccgtt ggtcgtgatg atcgtgccct ggttgaccac
       61 cgagccagca ctgcccgagt tatagaagtt gtagcgcccg gcgaggaagt cctggtcggt
      121 gatcgacagc gaggtcgcga acagggaccc gacgtcaacg ctcgcgctgg gggcgaagag
      181 cacgccgctc gggttggaga ggaacacctg cccgttggcg ctaagccggc cgaagatctc
      241 ggaggggttg ttgccgagta ccttgttgag ggcgaccgag gaagcgcttg gctggctgaa
      301 gttgacccag gcgctcgagc cgatcgagaa ggtctgccag ttgaggatcg ccttgtcggt
      361 ggcctggtcg atctgcatct tcgacccggc ggtcgagatg ttggccgtgc cggccgcgac
      421 ttgccctccg gtggggagct ggttcgccgc aggatccgcc aaggcgggtt gcatccacac
      481 ccaaggcatg agcgctgcgc tcaccgcgag caccgcggcg cgcggttggg ttcgcatggt
      541 ccgccgcgtg cgcgtccgaa tgctggctag attgccggga ctgttacgcg tcgttaccat
      601 ggtcctctcc ctaaacccat ccatgcatca ctcgcggcga ccgacccagt ctaggccgca
      661 cctcagccgc gggttgtgaa aaaaaccgta aataagcgta aaaaaagtca caacgcctca
      721 aaaccatttg atcgcctgta tccacacgcg cggcacgcgc ctcgccttgt ccgattccgg
      781 atcctgccgg gtgtacttcc acgccatgct tgcgcgcacc aggaagccgc cttccttgcc
      841 gagcgaagtg ccgaggccga cgcctgaaat atggcggata ttttcgccgt cggtcgacag
      901 cggaatctgg ttcaccttcg cccagccgac gtcgaagaaa gcgagaaacg ccatgtcgcc
      961 gtcgaatatc ttggtgccgg gaacgatgta gcgcggctcg accgtggcga tcatgccgct
     1021 gtccgccgtt gcctcgccta caggataggc gcgcacgccg ttcggtccgc cgagcgacat
     1081 tttttccgcc gaggccaggt tgcgcgacgc ccattggtgg gagaacgaaa acaatatgct
     1141 cgcgtcgttg ctgacgcgct gctgccgccg aaggtcgtag ttcgtcttgt ggaaatttcc
     1201 ctccgtctgg cggccagtgg ccgcgaggtc cgctgattcg gcagccaccg ggctaaggcc
     1261 aagattgccg aatgtataag ccagcgaata cgcattcagg ccgccgccga gaattgcgtc
     1321 ccggaagtcg cccaccgcgg ccaccttcgt ggtttggatg ttgctctgct tgagagaagc
     1381 cgggttttcg atgcggtcga tgagctgctt ctcttcatag gccacgttga ggatcaggtt
     1441 ggtgttccgc gtgcggacga tcggatggaa ggcgtagagg cctttcacct cgccttcgcc
     1501 gttcgcgccc agcgaagcaa agtccttgga cagcgcgtag tcgaatttcg agtagctcac
     1561 gccgacgcgc gtgccgtagt aaccgacggg cagatcgtag gcgaggcgcg cgtaccacat
     1621 cttctcgtcg gtggtgaagg cacgcagcgt tgcccggtcc ccatagccgg caggattgct
     1681 caggttcaag ttcgcgccga ggcggtattc gccggtgaag cggttgccgt aattgtcgat
     1741 atcgacgaaa ccgttgaagg agccggcgcc tggatcgacg tttaccctga ggttcgcggc
     1801 gccgaccgtt tcgcccgggc gcagctcggt ggtcacgctc gcatcgggca ggtcgttgat
     1861 gaggagcagc gggcgctcga gcccggtctc cgtgatgatg tcgctctgct gcagatgcgc
     1921 gttgatgatg cccgcgagca gccgcgtgct gatctgcgtc gcgggcttgg tgttgagctc
     1981 gattacgtcc cagcggccct cgatgatggt gatctcgacc gagccgccgc ggatcgcctg
     2041 ctgcggcagg taggcctgcg ccagaaagta gccgcgcgag cggtaatacg cgcgcgcttc
     2101 ggtcgcggcg tcgttcagcc cttcgaagtt gagctccttg ccgacgaact ccttcaccgc
     2161 ttcttgcagc tcgtcctccg ggatcagcgt gttgccggag aaggtgaagc gcgaaacctt
     2221 cactttcagc tgcggttgcg cgccgagcgc gggcttcggc tcgggcggct tcggacgcag
     2281 gtcgggcgac ggcggcggca ggcgcaacgg ctcgcgcgtc tgctcgagga tctgtcctgc
     2341 gtcgggtttc tgctgcgcga gcaccagcgc actcgcaact gaaaacacca cgaatgcgac
     2401 caggcgatga agactcattt ctcttgctcc gttccgtttc tttgtctatc cgtcgccggc
     2461 gcacgacagg gcgcgcacgc aacgtcgctt tttggaactg cggcgagcaa gggaaagacc
     2521 aacgcgcgac gcgccgcgca aagacaatcg cgcggacctc cgctgacccc cggctctgtt
     2581 gcctcccagc ttgtcaattc ttctacctga ttcaacagct tgcggctgtt ttttgtacct
     2641 cggcttggga aaccaagcct gcccgcggca gaatatcgtg atttattacc gttcgcaacg
     2701 aaaagccggg gcgaaatagc gcttgttgcg ctacggcaac cgaaaacccc cgcccgagca
     2761 agacaaaaag acaaaattcc gcggaatctt tttcaaaagt ttacgttcca ctgaccgccg
     2821 ccggctcgcc cttcggcacc cagatcacga aaaggttggc gatcacgatt gttccggcaa
     2881 ggaagtagaa cgtcgcgagc aggccgtagt ggtccgccac catgccgccg agcagcggcc
     2941 cgagcgagcc gccgatcgcc tgcgcgccga acagcacgcc gatgctcgat ccgcccatgt
     3001 tcttcggcgt ggtctcgagc agccaggcct ggatcaccgg ccgcgtggcg tagaggaaga
     3061 agccgagcac cgcgaccagg ccgatgaaaa gcggcgagcc gccggcgaac gccatcagcg
     3121 cgagcaccac cgcgctcgta gccatgctgc ccatgagaat gtgcttgcgc cccatgcggt
     3181 cggagaggtg cccggccacg ggtgcggcgg caaagcccgc ggcttgcagc gcgaacaggc
     3241 aggctccgat ccaggcaggc gaataaccca tggaattcgc gaggaacacc ggcaggaagg
     3301 tgagcagcgc ggtctgcgtc atggtacgga acgacgagcc ggcgcagagc agcagcagcg
     3361 cgcggttgcg gaacaggctc cgcagcccgc ccaggtatgc gcgcagcgat tggctctcgc
     3421 catgggactt cttcgcggaa agctgcagcg tgccgaggaa cgcgaagatc agaagcgcga
     3481 cgaccaggcc gggaagcaca ttcatgacga cgacctggc
//
LOCUS       DASPXW010000278         3591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_711109, whole genome shotgun sequence.
ACCESSION   DASPXW010000278 DASPXW010000000
VERSION     DASPXW010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711109"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..964)
                     /locus_tag="VEL04_06340"
     CDS             complement(20..964)
                     /locus_tag="VEL04_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HYT48022.1"
                     /translation="MSNMYDAVGLCVPYGRANSQYETQFEPATGTVWGYFNPKEGAPC
                     YSLGLLKDILEHDRQLVANQGHVEFDGMLHAVQQYVTASRVPGVFNLGGDLALFSLLI
                     KTGDREALAQYAKLCIDNMYPRTQSFFSPTLTKICLVQGDALGGGFECALASDLIVAE
                     ETARLGFPEILFNLFPGMGACSLLSRRIGIRAAEELILSGQVLPAYKLLDMGVVDVLA
                     KEGEGETAVREWIARNAKRRNGMQAVFRARQFVHPVTREELDGIVGLWVDAALRLGER
                     ELKMMNRLARAQMRRMESESFVDAELAPRQAEPVALAV"
     gene            1152..1388
                     /locus_tag="VEL04_06345"
     CDS             1152..1388
                     /locus_tag="VEL04_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48023.1"
                     /translation="MYAIVYKSDGFPICRQVAGVSPDPVVTWNNEAAAKAFISSKGKD
                     AEFQPLQLTDEAMDKLAKIIGFPIETMTFEPYPG"
     gene            complement(1531..1722)
                     /locus_tag="VEL04_06350"
                     /pseudo
     CDS             complement(1531..1722)
                     /locus_tag="VEL04_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012839646.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
     gene            complement(1722..2387)
                     /locus_tag="VEL04_06355"
     CDS             complement(1722..2387)
                     /locus_tag="VEL04_06355"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HYT48024.1"
                     /translation="MHAPVNRSKKSLDKFRYLVVEGPIGAGKTSLARRLAARLSADLL
                     LEQPQENPFLARFYDDMPRYALPAQLFFLFQRARLLEPLAQLDLFARPAIADFLLDKD
                     PLFARITLSADELALYQKIYDALRPRSPAPDLVVYLQAQPAILVERVRRRAAGYERGI
                     SEEYLALLAESYARFFYHYDAAPLLIVNSENLNFVERDDDFELLVTRLRSMKSRREFF
                     NLG"
     gene            complement(2347..2829)
                     /gene="folK"
                     /locus_tag="VEL04_06360"
     CDS             complement(2347..2829)
                     /gene="folK"
                     /locus_tag="VEL04_06360"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015065009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HYT48025.1"
                     /translation="MTLAYVGIGSNLGEPAKQVESAFEELGRIRQTSLAARSSLYRSQ
                     PVGYADQPEFFNAVAALETSLEPLELLRELQAIEARHGRRRSFANAPRTLDLDLLLHG
                     QAVVRRPELALPHPRMHQRCFVLKPLLEIAPAVEIPGIGAAQARLDACAGQSVEKIVG
                     "
     gene            complement(2826..>3591)
                     /locus_tag="VEL04_06365"
     CDS             complement(2826..>3591)
                     /locus_tag="VEL04_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HYT48026.1"
                     /translation="DPVRMLRAVRLAAKLGLTLDAATREPIRSMAPLMERVPPARLFD
                     EMLKLLLSGHASACLRQLREVGLHQGLLPLLDVILEQPLGERFVTLALAQTDERVRAE
                     RPVSPAFLFAALLWHEVLAAWKLRQGRGERSIPALESAMDEVLDTQSAKLAITRKLTA
                     TMREVWAMQPRFEQRSGSRAYRLLEAARFRMAYDFLALRAASGEVPAEIEAWWRGFQS
                     ADAETRKAMLLPDTGPRKRRRRRRKKRPAAPAPQPA"
BASE COUNT          612 a         1250 c         1180 g          549 t
ORIGIN      
        1 aagccggact aggagcgaat cagaccgcca aggccactgg ctcggcctgg cgcggcgcga
       61 gctcggcgtc gacgaaggat tccgattcca tgcgacgcat ctgcgcgcgc gcaagccggt
      121 tcatcatctt cagctcgcgc tcgcccagcc gcagcgccgc gtcgacccac agcccgacaa
      181 taccgtcgag ctcctcgcgc gtcaccgggt gaacgaactg ccgcgcgcgg aacaccgcct
      241 gcatgccgtt gcggcgcttc gcattgcgcg cgatccattc gcgcaccgcg gtttcgcctt
      301 ccccttcctt ggcaagcacg tccaccaccc ccatatcgag cagcttgtac gcgggcagca
      361 cctgacccga gaggatcaat tcctcggcgg cgcgtattcc gatgcggcgc gacagcaggc
      421 tgcaggcgcc catccccggg aagaggttga acaggatctc cgggaagccc agccgcgcgg
      481 tctcctctgc gacgatgagg tcggaggcga gcgcgcactc gaagccgccg ccaagcgcgt
      541 cgccctgcac caggcagatc ttggtgagcg tcgggctgaa gaagctctgg gtgcgcgggt
      601 acatgttgtc gatgcacagc ttcgcatact gcgcgagcgc ctcgcgatcg cccgtcttga
      661 tcagcagcga gaacaacgcc aagtctccgc cgaggttgaa tacgccgggc acccgcgagg
      721 cagtcacgta ctgctgcacc gcgtgcagca tgccgtcgaa ctcgacatgg ccctggttcg
      781 ccaccagttg ccgatcgtgc tcgaggatgt ccttcagcag gcccaggctg tagcagggcg
      841 cgccttcctt cgggttgaaa tagccccaga ccgttccggt agcgggctcg aactgcgttt
      901 cgtattgcga attggccctg ccgtaaggca cgcagagacc tacagcgtcg tacatgttgc
      961 tcatttacgc tctcccagta atgccccttg gcggggcgtc atcgccttgg cgcctgtacg
     1021 gcaagagccg agcctgcgcc acccggctcg attaggcgcc tcgcggaagc ggaagcgcaa
     1081 tcagggcttc ccctatgtcg tcgccttgtt gccccaccgg cacatcggtt aaattagggt
     1141 tgggggggcg catgtacgcc attgtctaca aatccgacgg attcccgatc tgccgccaag
     1201 tggccggcgt gagccctgat ccggtcgtca cctggaacaa cgaagccgcc gccaaggcgt
     1261 tcatctcgtc gaaggggaaa gatgcggaat tccagccctt gcagctcacc gacgaggcga
     1321 tggacaagct cgccaagatc atcggcttcc cgatcgagac gatgaccttc gagccctacc
     1381 cgggctagaa ctcgaggctg tcaatcaggc gtgtcgtccc gagccgcgcc gcggcgacga
     1441 ctacccgctc ccggtcgtcc gattgcggca gtgcaagatc gccccgccgt cgcacctcca
     1501 gatagtccgg ccgccagccc gccgccgcca gaagtcctcg ccgggcagga actgcagccg
     1561 gttgacgaag atgctcaccg ccacggcgcg ggcctggagg cgcgccaggc ggacgagcga
     1621 aagatgcccg gcgtgcagat tgcccatggt gggcaccaac gccggcggcg aggcggcggc
     1681 cagcgcacgg cgcagcgaac cgacctcggc tacgatccgc atcagccgag gttgaagaat
     1741 tcgcgccggc tcttcatgct gcgcagccgc gtgacgagca gctcgaaatc gtcgtcgcgt
     1801 tccacgaagt tgagattctc tgagttaacg atcagcagcg gcgccgcgtc gtaatgataa
     1861 aagaagcgcg catagctttc ggcgagcagc gccagatact cctcgctgat gccgcgctcg
     1921 tagccggcag cgcgccgccg cacccgctcc accaggatcg ccggctgcgc ctgcaggtaa
     1981 accaccagat ccggcgccgg agagcgcggc cgcagggcgt cgtagatctt ctggtagagc
     2041 gcgagctcgt cggccgagag cgtgatgcgc gcgaacagcg ggtccttgtc gagcaggaag
     2101 tccgcgatcg ccggccgcgc aaacagatcg agctgcgcca gcggctcgag cagccgggcg
     2161 cgctggaaca ggaagaaaag ctgcgccggc agcgcgtaac gcggcatatc gtcgtagaag
     2221 cgcgcgagaa acggattctc ctgcggctgc tcgagcagaa gatccgccga gagccgcgcg
     2281 gcgaggcgcc gcgcgaggct cgtcttgccg gcgccgatcg gcccttccac caccaggtag
     2341 cggaacttat ccaacgattt tttcgaccga ttgaccggcg catgcatcga gccgcgcttg
     2401 cgcagcgccg atgccgggga tctcgaccgc gggagcgatc tcgagcagcg gcttcagcac
     2461 gaaacagcgc tggtgcatgc gcggatgagg cagcgccagt tccgggcggc gcacgacggc
     2521 ttgcccgtgg agcagcaggt ccaggtccag ggtgcgcggg gcgtttgcaa agcttctgcg
     2581 gcgcccgtgg cgcgcctcga tcgcctgcag ctcccgcagc aactccaggg gctcgagaga
     2641 ggtttccaac gccgccaccg cattgaagaa ctcgggctgg tcggcgtagc caacgggctg
     2701 cgagcgatac agggacgatc ttgccgccag gctcgtttgc cggattcggc ccagctcctc
     2761 gaacgcggat tcgacctgct tggcgggctc gccgaggttc gagccgatac ccacgtacgc
     2821 gagggtcacg caggctgggg cgcgggggcg gccggccgct tcttgcgccg ccgccggcgc
     2881 cgcttgcgcg ggccggtatc gggcagcagc atcgccttgc gcgtctcggc gtccgcgctc
     2941 tggaatccac gccaccatgc ctcgatctcg gcgggcacct cgccgcttgc cgcgcgcagc
     3001 gccaggaagt cataggccat gcgaaagcgc gcggcctcca gcagccggta cgcgcggctc
     3061 cccgagcgct gctcgaagcg cggctgcatc gcccagacct cgcgcatggt ggcggtgagc
     3121 ttgcgggtaa tcgcgagctt cgcgctctgc gtgtcgagca cctcgtccat tgcgctctcg
     3181 agcgccggga tgctgcgctc gccgcgtccc tgccgcagct tccacgccgc gagcacctcg
     3241 tgccacagca gcgcggcgaa caggaacgcc ggcgacacgg gacgctcggc gcgcacgcgc
     3301 tcgtcggtct gcgcgagcgc cagcgtgacg aagcgctcgc cgagcggctg ctccagaatc
     3361 acgtcgagca gcggcagcag cccctggtgc agcccgactt cgcgcagctg cctcaggcag
     3421 gcgcttgcat gccccgagag caacagcttc agcatctcgt cgaacagacg cgcgggcggc
     3481 acgcgctcca tcagcggcgc catgctgcgg atcggctcgc gcgtcgccgc gtcgagagtc
     3541 agcccgagct tcgcggcgag ccgcaccgcg cgcagcatcc tcaccgggtc c
//
LOCUS       DASPXW010000279         3312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_961269, whole genome shotgun sequence.
ACCESSION   DASPXW010000279 DASPXW010000000
VERSION     DASPXW010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961269"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..985
                     /locus_tag="VEL04_06370"
     CDS             71..985
                     /locus_tag="VEL04_06370"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HYT48027.1"
                     /translation="MTATAISTDAAPARRAKYGSMSRDRRWALRWSYVFLVLFAVFFL
                     MPPVYMLITSLKTSQEISAATNPWWVQNPTLGNYIELLTSSTYLTFFRNSALVSLLVV
                     LITMAVSIPAAFALSRMRFWGSATLATGVFLTYLVPETLLFIPLFKTFVAVTDVTGIE
                     LINRWWVLVLLYPTLTVPFCTWIMIGYFASIPKELDEAALIDGAGYLQILTKIFMPVA
                     LPGIIAATIFAFTVSWAQFLYPLAFTTSIDQLVLPVGIITTLIRGDVFTWAQIMTGAL
                     LGAAPPLVIYAFLMDYYIAGLTAGATKG"
     gene            993..2093
                     /gene="ugpC"
                     /locus_tag="VEL04_06375"
     CDS             993..2093
                     /gene="ugpC"
                     /locus_tag="VEL04_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HYT48028.1"
                     /translation="MAQVTLRRLMKKYDDTFAVRGIDLDIADKEFVVLVGPSGCGKST
                     TLRMIAGLEDVSEGEVLIGGEEVTDVPPKDRNIAMVFQNYALYPHMSVYENMSFGLRL
                     KKFPRHEIARRVENAARILGISELLERKPRQLSGGQRQRVAMGRAIVRDPKVFLFDEP
                     LSNLDAKLRVQMRTEIKKVHQKVRTTTVYVTHDQVEAMTLADRVVVMNGGLIEQIGTP
                     AELYHSPATGFVAGFIGSPAMNLMACALEEAAGGLRLRLADVLAFPVPAERTARYRPH
                     VGKAELLFGLRPEHIADARGPLEPNRHAFEIPVEVAEPMGMETLVYFPVQDFQVCARV
                     SSAAGAAAGARLKLAADLNHMHLIDARSGRVL"
     gene            2126..3052
                     /locus_tag="VEL04_06380"
     CDS             2126..3052
                     /locus_tag="VEL04_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYT48029.1"
                     /translation="MLAITVTAAAAQPSSGVIRVVVPFAAGGVQDIVARSFNAELGAA
                     LGRTVIVENRPGAGGTIGNASVARSAPDGGTLLLAAASHTITGHVYAKLPYHPIDDFT
                     PIAHIGNVDYVLMISAAVPARNIKEFIEYAKANPGKLNYASAGNGSATHLSMAYFAGL
                     AGLDMVHVPFKATSEAVNEVLAGRAHAVIAANIGAIPFAKDARIRMLGTTGRQRSKFL
                     PELPAIDESLPGYEFDSWIGLLGPAAMPKAMVEQINAAIAGLLKNPAILDRLDKHGVV
                     PQAMSPAEFNALLRADYAKMAKVVRASGARID"
BASE COUNT          538 a         1112 c         1074 g          588 t
ORIGIN      
        1 tggttccgat cctctccgtg gccgccgtgt tcattctccg cgacatccac cgccgcgcga
       61 gcgaaatgtc atgaccgcca cggcaatcag caccgacgcc gcgcccgccc gccgggcgaa
      121 atacggcagc atgagccgcg accggcgctg ggcgctgcgc tggtcgtatg tcttcctggt
      181 gttgttcgcg gtgttctttc tgatgccgcc ggtctacatg ctgatcacct cgctcaagac
      241 cagccaggag atctcggccg cgaccaatcc gtggtgggtc caaaacccga cgctgggcaa
      301 ttacatcgag ctcctgacct cgagcaccta cctcaccttc ttccgcaatt cggcgctggt
      361 gtcgctgctg gtggtactga tcaccatggc ggtcagcatc ccggcggcgt tcgcgctctc
      421 gcgcatgcgc ttctggggct cggccacgct cgcgaccggc gtgttcctca cctacctggt
      481 gcccgagacg ctgctcttca ttccgctctt caagactttc gtcgcggtga ctgacgtcac
      541 gggcatcgag ctcatcaacc gctggtgggt gctggtgctg ctctatccca cgctcacggt
      601 gccgttctgc acctggatca tgatcggcta cttcgccagc atcccgaagg agctcgacga
      661 ggcggcgctg atcgacggcg ccggctacct gcagatcctc accaagatct tcatgccggt
      721 ggcgctgccc ggcatcatcg cggcgaccat cttcgccttc accgtgtcgt gggcgcagtt
      781 cctctacccg ctcgccttca ccacctcgat cgaccagctg gtgctgccgg tcggcatcat
      841 caccaccctg atccgcggcg acgtcttcac ctgggcccag atcatgaccg gggcgctgct
      901 cggcgccgcg ccgccgctcg tgatctatgc gttcctgatg gattactaca tcgccggcct
      961 caccgccggc gcaaccaaag gctgagccct ccatggccca ggtgacgctg cgcaggctga
     1021 tgaagaagta cgacgacacc ttcgcggtgc gcggcatcga cctcgacatc gccgacaagg
     1081 agttcgtggt gctggtcggg ccttcgggct gcggcaagag caccacgctg cgcatgatcg
     1141 ccgggctcga ggacgtcagc gaaggcgagg tgctgatcgg cggcgaggag gtgaccgacg
     1201 tgccgcccaa ggaccgcaac atcgcgatgg tgttccagaa ctacgcgctc tacccgcaca
     1261 tgagcgtgta cgagaacatg tccttcggcc tgcgcctgaa gaaattcccg cgccacgaga
     1321 tcgcgcggcg ggtggagaac gcggcgcgca tcctcggcat cagcgagctg ctggagagga
     1381 agcccaggca gctctcgggc ggccagcgcc agcgcgtggc gatggggcgc gccatcgtgc
     1441 gcgaccccaa ggtgttcctg ttcgacgagc cgctgtccaa cctcgacgcc aagctgcgcg
     1501 tgcagatgcg caccgagatc aagaaagtgc accagaaagt gcgcaccacc acggtctacg
     1561 tgacgcacga ccaggtcgag gcgatgacgc tcgccgatcg cgtggtggtc atgaacggcg
     1621 gcctgatcga gcagatcggc acgccggccg agctgtacca ctcgccggcg acgggattcg
     1681 tcgccggctt catcggctca ccggcgatga acctcatggc ctgcgccctc gaagaggcgg
     1741 cgggcgggct aaggctgcgg ctcgccgacg tgctggcgtt cccggtgccg gccgagcgca
     1801 ccgcgagata ccggccgcac gtcggcaagg ccgagctgct tttcgggctg aggccggagc
     1861 acatcgccga cgcgcgcggc ccgctcgagc cgaaccggca tgcgttcgag atcccggtcg
     1921 aggttgccga gccgatggga atggagacgc tggtttattt cccggtgcag gacttccagg
     1981 tctgcgcccg ggtcagctcg gccgcgggcg cggcggccgg cgcgcgcctc aagctcgctg
     2041 cggacctcaa ccacatgcat ctcatcgacg cccgcagcgg gcgggtgctg tagcgatgaa
     2101 ggcaattcgc ggggtttggg ggtttgtgct ggccatcacc gtcacggcgg ccgcagcgca
     2161 accttcttct ggagtgatcc gtgtggtggt gccgttcgcc gcgggcggcg tgcaggacat
     2221 cgtggcgcgc tcgttcaacg ccgagctggg cgcggctctc gggcgcaccg tgatcgtcga
     2281 gaaccgcccc ggggcaggcg gcaccatcgg caacgcgtcg gtggccagga gcgcgccgga
     2341 cggcggcacg ctgctcctcg ctgcggcgag ccataccatc accggccatg tctacgccaa
     2401 gctgccttac cacccgatcg acgacttcac gccgatcgcg cacatcggca acgtcgacta
     2461 tgtgctgatg atcagcgctg cagttcctgc gcgcaatatc aaggaattca tcgaatatgc
     2521 aaaggcaaac ccgggcaaac tgaattatgc gagcgccgga aacggcagcg cgacgcatct
     2581 ctcgatggcc tactttgcgg ggctcgccgg cctggacatg gtgcacgtgc cgttcaaggc
     2641 gaccagcgag gcggtgaacg aagtgctcgc cggccgcgcg cacgcggtga tcgcggcgaa
     2701 catcggcgcc atccccttcg ccaaggacgc gcgcattcgc atgctgggga cgaccggaag
     2761 gcaacgctcg aaattcctgc ccgagctgcc ggcgatcgac gagagcttgc ctggctacga
     2821 gttcgattcc tggatcgggc tgctcgggcc ggcggcgatg ccgaaagcga tggtcgagca
     2881 gatcaacgcc gcgatcgccg ggctcctcaa gaacccggcg attctcgatc gcctggacaa
     2941 gcatggtgtc gtgccgcagg cgatgagccc tgcggagttc aacgcgctgc tgcgcgccga
     3001 ctacgccaag atggcgaagg tggtgagggc gtcaggcgcg cgcatcgact gacagcgtgc
     3061 tgagccttgt tgcatcgacg atcgtctttt ttgcctcgcg ctggctgtgg cttacggcgt
     3121 tgcggtgctc gtcgattttc tcgcttcctg actacacccc tcactggccg agcggcacgt
     3181 cctcgagcgg ggcgaacttc ggaatgacga ccgtaagcgt cttcacgttc tgcggcggcg
     3241 ccacgaattt ggcccagaac tcggtgcgtg tcttcgcctt gacgttgttg ctcgggctcg
     3301 agaccgcttt gc
//
LOCUS       DASPXW010000280         2870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1468089, whole genome shotgun sequence.
ACCESSION   DASPXW010000280 DASPXW010000000
VERSION     DASPXW010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1468089"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VEL04_06385"
     CDS             complement(<1..236)
                     /locus_tag="VEL04_06385"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001529.1"
                     /note="involved in translesion DNA polymerization with
                     beta clamp of polymerase III; belongs to Y family of
                     polymerases; does not contain proofreading function;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HYT48030.1"
                     /translation="MDAFYASVEQRDDPRLRGKPVAVGGSPQSRGVVAAASYEARGFG
                     VRSAMPMARALRLCPKLIIVRPDFRTYRAVSEEIR"
     gene            complement(281..1120)
                     /locus_tag="VEL04_06390"
     CDS             complement(281..1120)
                     /locus_tag="VEL04_06390"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HYT48031.1"
                     /translation="MKAQSIANDMAYDPGVALDFFKSAGKAEAVAQGATIFAEKEHEI
                     PLLRRHKMYLLLKGDVGLLTRGKPIGAVKAGEIFGELALISHAPRSAAAVAKTDCRVI
                     ALDEQEFHGALARKPAFALMLMSMMIRRLRETITQLSASGALSKDEAWKEAAAFNRTQ
                     LAALMRGLADDPPLYYRAGDAILARGQTGLRMYAVLEGRVAVSIDGRVIEKLGPGGVF
                     GEAAIIGQSARLASAQAETDCELLPIGRDPFLSLVKLSPEFADTMLTSLAERLRFLTA
                     RLN"
     gene            complement(1186..2406)
                     /locus_tag="VEL04_06395"
     CDS             complement(1186..2406)
                     /locus_tag="VEL04_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYT48032.1"
                     /translation="MLAALAAALLAIPFQDAAAQAAPLRLGFLTVRTGPLAAGGRQME
                     EGINLLLKERNNTFAGRKVQIIFADTAGQPALARTKTQELVERERVHAIIGPLATFEA
                     LAIDEYMLQARVPLITPTSAAQNDLAQQKKSDYVIHVYGTAAQPMYALGDYAAKQLGL
                     KRIAMIADDFTYGHEGAAGFHRVFEDGGGRVVQKLWPPLNVPDYGSFIGQLKTNVDGI
                     YAGFAGSNPLRFLRAYKEYGLRLPLFGNPTFVDEGILKNMGDEALGVYSASWYTVDRE
                     APDNRRFVESVQREYKVTPGFYTAGTYTAGLWLEEALKLVKGRFEEKSAFVRALHEVK
                     LEHGPMGPIRLGEYGKPILNIYIRKVERKRGQLVNTTIGTYPEVSQFWSYDPQQFIAG
                     PQYSRDVPAAKFLE"
     gene            2496..>2870
                     /locus_tag="VEL04_06400"
     CDS             2496..>2870
                     /locus_tag="VEL04_06400"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HYT48033.1"
                     /translation="MAFRNSIALVVPAVLCLAALDAAAQAPDAGTMYVVTYVELKPTA
                     SADAATLLKAYRDALRQGDGNLRAEVLRHTSRPGQFVVLSAWKDQKAFEAQRDAARNK
                     ELRERLQALRNSPADDRIHVALS"
BASE COUNT          430 a          986 c          978 g          476 t
ORIGIN      
        1 cggatctcct cggagacggc acggtaggtg cggaagtcgg gacggacgat gatcagcttc
       61 gggcacaggc gcagcgcgcg cgccatcggc atcgccgagc gcacgccgaa gccgcgcgcc
      121 tcgtagctcg ccgctgccac cacgccgcgc gactgcggcg agccgccgac cgccaccggc
      181 ttgccgcgca gccgcgggtc gtcgcgctgc tcgaccgacg cgtagaacgc gtccatgtcg
      241 atgtggatga tgcgccgctc gcgcgcttcg gccacgccgg ctagttcagg cgcgcggtca
      301 ggaacctcag ccgctcggcg agcgaggtga gcatggtgtc ggcgaactcg gggctcagct
      361 tcaccagcga gaggaagggg tcgcggccga tcggcagcag ctcgcagtcg gtctccgcct
      421 gcgcgctcgc gagccgcgcc gactggccga tgatcgccgc ctcgccgaac acgccgcccg
      481 ggccgagctt ctcgatcacg cggccgtcga tcgacaccgc gacgcggccc tcgagcaccg
      541 cgtacatgcg caggccggtc tgccctctgg cgaggatcgc gtcgccggcg cggtagtaga
      601 gcggcggatc gtcggccagt ccgcgcatga gagcggcgag ctgcgtgcgg ttgaacgcgg
      661 cggcttcctt ccaggcttcg tccttcgaca gcgccccgga cgccgagagc tgcgtgatgg
      721 tctcgcgcag ccgccggatc atcatgctca tcagcatcag cgcaaacgcg ggcttcctgg
      781 cgagcgcgcc gtggaactcc tgctcgtcga gcgcgatcac ccggcaatcg gtctttgcca
      841 ccgccgcggc gctgcgcggc gcgtggctga tgagcgccag ctcgccgaag atctcgccgg
      901 ctttcaccgc gccgatcggc ttgccgcgcg tcagcaggcc gacatcgccc ttgagcagca
      961 ggtacatctt gtgccgcctg aggagcggga tctcgtgttc tttctcggcg aaaatggtgg
     1021 cgccctgcgc cacggcttcc gccttgcccg cagacttgaa gaagtccagc gccaccccgg
     1081 ggtcgtacgc catgtcgttt gcgatgcttt gcgctttcat ccgtttctcc tcgcaggcat
     1141 aagatgccat gaaatcagcg aggccgaccg gcaaaattgc cgcctttatt cgaggaactt
     1201 cgccgccggg acgtcgcggc tgtactgcgg cccggcgatg aactgctgcg ggtcgtagct
     1261 ccagaactgg ctcacctcgg gataggtgcc gatggtggtg ttgacgagct ggccgcgctt
     1321 gcgctcgacc ttgcggatgt agatgttgag gatcggcttg ccgtactcgc ccaggcggat
     1381 cggccccatc gggccatgct cgagcttcac ctcgtgcagc gcgcgcacga aggcgctctt
     1441 ctcctcgaag cggcccttca ccagcttcag cgcctcctcg agccacaggc cggcggtgta
     1501 ggtgccggcg gtatagaagc ccggcgtcac cttgtactcg cgctggaccg actcgacgaa
     1561 gcgccggttg tcgggcgcct cgcggtcgac cgtgtaccag ctcgccgaat acacgccgag
     1621 cgcctcgtcg cccatgttct tcaggatgcc ctcgtcgacg aaggtcggat tgccgaacag
     1681 cggcaggcgc aggccgtact ccttgtacgc gcgcaggaag cgcagcgggt tcgagccggc
     1741 gaagccggcg tagatgccgt cgacattggt cttcagctgg ccgatgaacg agccgtagtc
     1801 gggaacgttg agcggcggcc acagcttctg caccacgcgc ccgccgccgt cctcgaacac
     1861 gcggtgaaac ccggccgcgc cttcgtggcc gtaggtgaag tcgtcggcga tcatcgcgat
     1921 gcgcttgagc ccgagctgct tcgccgcgta gtcgccgagc gcgtacatcg gctgcgccgc
     1981 ggtgccgtag acgtggatca cgtagtcgct cttcttctgc tgcgcgaggt cgttttgcgc
     2041 cgcggaagtc ggcgtgatga gcggcacccg cgcctgcagc atgtactcgt cgatcgcgag
     2101 cgcctcgaac gtggcgagcg gcccgatgat ggcgtggacc cgctcgcgct cgaccagctc
     2161 ctgggtcttg gtccgggcga gcgccggctg gcccgcggtg tcggcgaaga tgatctgcac
     2221 cttgcgcccg gcgaacgtgt tgttgcgttc cttgaggagc aggttgatac cttcctccat
     2281 ttgcctgccg cccgcggcga gcggcccggt gcgcaccgtg aggaagccga ggcgcagcgg
     2341 cgcggcttgc gccgccgcat cctgaaaggg gattgcaagc agggcggccg ccaacgcggc
     2401 cagcaccagc gaacgtcgaa gatttttcat ggctgtcccc cctccaatgt gagtaccata
     2461 tccggctgct gcgcggatga acaggggagg aaaccatggc attccgcaat tcgatcgcgc
     2521 tcgtcgtgcc ggcggtgttg tgccttgccg cgcttgacgc ggctgcccag gcgccggacg
     2581 cgggcaccat gtacgtcgtc acctacgtcg agctgaagcc cacggcgagc gccgatgcgg
     2641 cgacgctgct gaaggcctat cgcgacgcgc tgcgccaggg cgacggcaac ctgcgcgccg
     2701 aggtgctgcg gcacaccagc cgcccgggcc agttcgtggt gctctcggcc tggaaggacc
     2761 agaaggcctt cgaagcgcag cgtgacgccg cgcgcaacaa ggagttgcgc gagaggctcc
     2821 aggcgctgcg caacagcccc gccgatgacc gcatccacgt cgcgctctcg
//
LOCUS       DASPXW010000281         4051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_587126, whole genome shotgun sequence.
ACCESSION   DASPXW010000281 DASPXW010000000
VERSION     DASPXW010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587126"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..986)
                     /gene="trxB"
                     /locus_tag="VEL04_06405"
     CDS             complement(33..986)
                     /gene="trxB"
                     /locus_tag="VEL04_06405"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HYT48034.1"
                     /translation="MPMRHARLLILGSGPAGYTAAVYAARANLRPVLITGIAQGGQLM
                     TTTDVDNWPADADGVQGPDLMERFRRHAERFETEVISDHIHTVRLAQHPFVLEGDAAT
                     YTCDALIVATGATAKYLGLASEQKFMGRGVSACATCDGFFYKGQDVVVVGGGNTAVEE
                     ALFLANIARHVELVHRRDKFRAEKIMQDKLMKRVADGTIGLVLDAVVEEVLGDGSGVT
                     GVRVNRLKKSAKQDLAAKGLFVAIGHTPNTQIFAGQLEMKDGYILTRGGAEGLATATS
                     VPGVFAAGDVQDHVYRQAVTSAGSGCMAALDAEKYLDGLAA"
     gene            1055..1723
                     /locus_tag="VEL04_06410"
     CDS             1055..1723
                     /locus_tag="VEL04_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HYT48035.1"
                     /translation="MATTVSTAVLKAVPLFASFPEDQLRVLATMVIRKSAPRSNTIMA
                     GGDPTDSLYIVLSGRLKVMMSDSDGKEVILSILGPGEFFGEMGLIDEAPRSASVVTIE
                     PCELLQLAKRDFNKCLAENFEMAMAVMRGLVRRLREADRKIGSLALLDVYGRVARLLL
                     DMAETVDGEKLVTKRLPKQDIAKMIGASREMVSRVMKDLQMGGYIEMRGSIIVLRDIV
                     MLPE"
     gene            1733..3988
                     /locus_tag="VEL04_06415"
     CDS             1733..3988
                     /locus_tag="VEL04_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HYT48036.1"
                     /translation="MGAAEKSASALPAKLANLLREAKWLGLVAAALYLLLIFVTFDRA
                     DPGWSHSATGAAARNAGGVVGAWLADLLLYVFGLSAYWWVALCVYVVAWGYRGLDDRR
                     LVDRRPLTLAFAGFVVLITASAGLESLRLHTLPVALPLAPGGVVGAEAGRLAAEALGF
                     TGATLVLIALAAVGFSQFTGLSWLRVSEGVGLALEAAYTLALRAWERRQDRRLGERAR
                     EERSLVVQTEKKREEDHPPLRIEPPLGEIRKSERVQKEKQAPLFEDLPDTPLPPLKLL
                     DEARRDAAQVSAETLEFTSRLIEKKLSDFGVVVKVLAAYPGPVITRYEIEPAVGVKGS
                     QIVNLVKDLARALSVVSIRVVETIPGKSCMGLEIPNPHRQTVRLSEILGSEAYHALHA
                     PLALALGKDIAGNPVVVDLARMPHLLVAGTTGSGKSVALNAMILSLLYKSELRQVRLI
                     LIDPKMLELSVYQDIPHLLVPVVTDMKQAVNALGWCVTEMDRRYKLMNWIAARNLSGF
                     NHKVTDREIEDPFSLEADKPQRLQPLPHIVIVIDELADLMMVVGKRVEELIARLAQKA
                     RAAGIHLILATQRPSVDVITGLIKANIPARIAFQVASKVDSRTILDGSGAETLLGAGD
                     MLYLPPGTGLAQRVHGAFVADHEVHHVVEHLKSLAKPEYLGEVLEPAPAEEAAAVDGE
                     PGGEKDALYDQAVEIVLRTRRPSISLVQRHLRIGYNRAARLIEDMERAGMVSAMQSNR
                     NREVLVPAKAE"
BASE COUNT          644 a         1337 c         1405 g          665 t
ORIGIN      
        1 ccccctgcag cgcctggcga aaatcctcgt cttcaggcgg caaggccatc gaggtacttt
       61 tcggcgtcga gcgccgccat gcagccgctg ccggcgctgg tgaccgcctg gcggtaaacg
      121 tggtcctgga catcgccggc ggcgaagacg ccgggcacgc tggtcgcggt cgccaggcct
      181 tcggctccgc cacgcgtcag gatgtagccg tccttcatct ccagctgccc ggcgaagatc
      241 tgggtattcg gcgtgtggcc gatcgctacg aacaggccct tggccgcgag gtcctgcttg
      301 gcgctttttt tcaaccggtt cacccgcacc ccggtgacgc cagagccatc gcccagcacc
      361 tcctcgacca ccgcatcgag cactaggccg attgtcccgt ccgccacgcg cttcatgagc
      421 ttgtcctgca tgatcttctc ggcgcggaac ttgtcgcgcc tgtgcaccag ttccacgtgg
      481 cgggcgatgt tggcgaggaa cagcgcctcc tcgaccgcgg tgttgccgcc gccgaccacc
      541 accacgtcct ggcccttgta gaaaaagccg tcgcaggtgg cgcaggccga gacaccgcgc
      601 cccatgaatt tctgctcgga ggcaagcccc aggtacttcg cggtggcgcc agtcgcgacg
      661 atcagcgcat cgcaggtata ggtcgccgcg tcgccttcga ggacgaaggg gtgctgggcg
      721 agccgcacgg tgtggatgtg atcggagatc acctcggtct cgaagcgctc ggcgtgcctg
      781 cggaagcgct ccatcagatc ggggccctgc acgccgtcgg cgtcggccgg ccagttgtcg
      841 acgtcggtgg tggtcatcag ctggccgcct tgcgcgatgc cggtgatcag caccggcctg
      901 aggttcgcgc gcgcggcata caccgccgcg gtgtagccgg ccggtccgga accgagaatc
      961 agaaggcgtg cgtggcgcat cggcattcgc atgctgctcg gacggggaat cgatcacgga
     1021 ttataatcgg cacccaggag ggacgattct cgatatggcg acgacagtct ccacggcggt
     1081 gctcaaggcg gtgccgctgt tcgccagctt cccggaagac cagttgcgcg tgctcgcgac
     1141 gatggtgata cgcaagagcg cgccgcgctc gaacaccatc atggcgggcg gcgatcccac
     1201 cgactcgctc tacatcgtgc tctccggccg gctgaaggtg atgatgagcg actccgacgg
     1261 caaggaagtg atcctcagca ttctcgggcc gggcgagttc ttcggcgaga tgggcctgat
     1321 cgacgaggcg ccgcgctcgg cgagcgtggt cacgatcgaa ccctgcgagc tcctgcagct
     1381 cgccaagcgt gatttcaaca agtgcctggc ggagaacttc gagatggcga tggcggtgat
     1441 gcgcggcctg gtgcggcgcc tgcgcgaggc cgaccgcaag atcggcagcc tggcgctgct
     1501 cgatgtttat ggccgcgtgg cgcggctgct gctcgacatg gccgagaccg tggatggcga
     1561 gaagctggtg accaagcgcc tgccgaagca ggacatcgcc aagatgatcg gcgcctcgcg
     1621 cgagatggtg agccgcgtga tgaaggacct gcagatgggc ggctacatcg agatgcgcgg
     1681 ctcgatcatc gtgctgcgcg acatcgtcat gcttcccgag tagcccgcga gggtgggcgc
     1741 agccgaaaaa tccgcaagcg cgctgcccgc aaagctcgcc aacctgctgc gggaggccaa
     1801 atggctgggg ctggtcgcgg ctgcgctcta cctgttgctg atcttcgtca ccttcgatcg
     1861 cgccgatccg ggctggtcgc atagcgcgac cggcgccgcc gcgcgcaacg ccggcggcgt
     1921 ggtgggcgcc tggctggcgg acctgctgct gtacgtgttc ggcctttcgg cgtactggtg
     1981 ggtggcgctg tgcgtctacg tggtggcttg gggctatcgc gggctcgatg accgccggct
     2041 cgtcgaccgc cggccgctca cgctcgcgtt cgccggattc gtggtcctga tcaccgcgag
     2101 cgccgggctc gagagcctgc gcttgcacac gctgccggtc gcgctgccgc tcgcgccggg
     2161 cggcgtggtc ggcgccgaag cgggcaggct cgccgcggag gcgctcggct ttaccggcgc
     2221 gaccctggtg ctgatcgcgc tcgctgcggt cggcttcagc cagttcaccg gcctgtcgtg
     2281 gctccgggtg tccgaaggcg tcggcctggc gctcgaggcg gcctacacgc tcgccctgcg
     2341 cgcctgggag cggcgccagg accgcaggct cggcgagcgg gcgcgcgagg agcgcagcct
     2401 ggtggtgcag acggaaaaaa aacgcgagga agaccatccg ccgctgcgca tcgagccgcc
     2461 gctgggcgag atcaggaagt ccgagcgcgt gcagaaagag aagcaggcgc cgctcttcga
     2521 ggacctgccc gacactccgc tgccgccgct caagctgctc gacgaggcaa gacgcgacgc
     2581 cgcgcaggtc agcgccgaga cgctggaatt cacctcgcgc ctgatcgaga agaagctctc
     2641 cgacttcggc gtcgtggtga aggtgctcgc ggcctacccg ggaccggtga tcacgcgcta
     2701 cgagatcgag cccgcggtcg gcgtgaaggg cagccagatc gtgaacctgg tgaaggacct
     2761 ggcgcgggcg ctgtcggtgg tgtcgatccg cgtggtcgag accatcccgg gcaaatcgtg
     2821 catggggctc gagatcccca atccgcaccg ccagacggtg cggctctccg agatcctcgg
     2881 ctccgaggcc taccacgcgc tgcatgcgcc gctcgcgctc gcgctcggca aggacatcgc
     2941 cggcaatccg gtggtcgtgg acctcgcgcg catgccgcac ctgctggtcg ccgggactac
     3001 gggctcgggc aagtcggtgg cgctcaacgc gatgatcctg tcgctgcttt acaagtcgga
     3061 gctgcgccag gtgcggctca tcctgatcga cccgaaaatg ctcgagcttt cggtgtacca
     3121 ggacattccg cacctgctcg tgccggtggt caccgacatg aagcaggcgg tgaacgcgct
     3181 cggctggtgc gtcaccgaga tggaccggcg ctacaagctg atgaactgga tcgctgcccg
     3241 caacctgtcg ggcttcaatc acaaggtcac cgaccgcgag atcgaggacc cgttcagcct
     3301 cgaggcggac aagccgcagc ggctgcagcc gctgcctcac atcgtgatcg tcatcgacga
     3361 gctcgccgat ctgatgatgg tggtcggcaa gcgggtcgaa gagctgatcg cgcggctcgc
     3421 gcaaaaagcc cgggccgctg gaatccacct gatcctcgcg acgcagcggc cgtcggtcga
     3481 cgtcatcacc gggcttatca aggcgaacat cccggcgcgc atcgctttcc aggtcgcctc
     3541 caaggtcgac tcgcgcacca tcctcgacgg ctcgggcgcc gagacgctgc tcggcgccgg
     3601 cgacatgctg tacctgccgc cggggacggg cctggcgcag cgcgtgcacg gcgccttcgt
     3661 cgccgaccac gaggtgcatc acgtagtcga gcatctgaag agcctggcga agcccgagta
     3721 cctcggtgaa gtgctggagc ctgcgcccgc cgaggaggcg gcagccgtcg acggggagcc
     3781 gggcggcgag aaggatgcgc tgtacgacca ggcagtcgag atcgtgctgc gcacgcgccg
     3841 gccgtcgatc tcgctggtgc agcggcacct gcgcatcggc tacaaccggg cggcgcgcct
     3901 gatcgaggac atggagcgcg ccggcatggt ttcggcaatg cagtcgaacc gcaaccgcga
     3961 ggtgctggta cccgcaaagg cggagtaggc cgccgcgatg cgcttccttg ttttactgac
     4021 aatattattt gtatcagcgg cgcaggccgc a
//
LOCUS       DASPXW010000282         5616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1092631, whole genome shotgun sequence.
ACCESSION   DASPXW010000282 DASPXW010000000
VERSION     DASPXW010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5616
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092631"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VEL04_06420"
     CDS             <1..259
                     /locus_tag="VEL04_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012492295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HYT48037.1"
                     /translation="QKIWRGGNSAAGVPACAGCHGPAGAGLPAQYPRLAGQYAEYVAA
                     QLKAFREGSRANDPNGAMRGVAIRLSEREIRAVAEYAAGLR"
     gene            328..783
                     /locus_tag="VEL04_06425"
     CDS             328..783
                     /locus_tag="VEL04_06425"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HYT48038.1"
                     /translation="MKKSLVAAGVAVAVASGVALAQVKPEILVKQRQAVMTLQGKYFG
                     PMAGMAQGKLPYDANVVQRNAGFLDNLSRMPWDGFDPSTKDVSVKTAALPAVWSDNAK
                     FREAADRLQSETSKLYAVSRSGDEAAVKAQIGAVGKACGGCHENFRQKQ"
     gene            850..1650
                     /locus_tag="VEL04_06430"
     CDS             850..1650
                     /locus_tag="VEL04_06430"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HYT48039.1"
                     /translation="MAFAQGDAKRGEYIAKAAGCVGCHTEAKKQAVPYAGGRALKTPF
                     GTFYGPNITPHPQAGLGRWSEADFMRAMREGRRPDGANYFPAFPYPSFTRITDTDLRD
                     LWAYLRTLAASSRPSRQHELGFFFRWRFLVGIWKWFFFSPGAFAPDAARSTILNRGAY
                     LVQALGHCGECHTPRNWFGAPKASRYLAGAKIGDATASNLTPTRLKKLSDAELREVLR
                     TGLTPDGDVLAESMAEVVQNTTGQLTQQDLDALLAYLRSLPPLPDGPK"
     gene            1686..2186
                     /locus_tag="VEL04_06435"
     CDS             1686..2186
                     /locus_tag="VEL04_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYT48040.1"
                     /translation="MNEAERLVELIPRLRRYARALVGDRASADDLVQDTLERAWAKLH
                     LYRRGTDLRAWLFTVMHNVHVNRVRAARATDPLDDEMPELAQRAAQGDALLVRDLDRA
                     IARLPTEQRAVLLLVTLEEMSYEDVARALGIPIGTVMSRLSRAREKLRFMMLGQSAAA
                     KLKVVK"
     gene            2183..2962
                     /locus_tag="VEL04_06440"
     CDS             2183..2962
                     /locus_tag="VEL04_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HYT48041.1"
                     /translation="MNEEQQPSESELQAYADGRIAAEQSAAIESWLAAHPDDAERIET
                     YRRLGEDLRARYDPVLEEAVPGQLRRVIGPMRRWHPVAKAAGWATLGGALGALAGWQL
                     HELRPTAPAASDSGAVMARRAAVAHATYSPEVRHPVEVGAEQEQHLIAWLSKRLGAKV
                     QAPKLDEAGMSLVGGRLLPGESGPVAQFMYQTASGRRATLYVRAEARGHRETAFRYAR
                     ENNVSVFYWIDRECGYALASADLSREELLRIATLVYKQLEP"
     gene            3102..3269
                     /locus_tag="VEL04_06445"
     CDS             3102..3269
                     /locus_tag="VEL04_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48042.1"
                     /translation="MSFLDVQDLATVAAQVDIARLISAWCCESDTLLAAVEAGLGACL
                     VAFVIRRKAKR"
     gene            complement(3436..3999)
                     /gene="mug"
                     /locus_tag="VEL04_06450"
     CDS             complement(3436..3999)
                     /gene="mug"
                     /locus_tag="VEL04_06450"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: protein motif:HMM:NF007570.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HYT48043.1"
                     /translation="MSRTRNRADTSSGRPSAADLAAAAGKGIKDVIAPGLRVLFVGIN
                     PGLYSAATGYHFARPGNRFWPALHAAGFTAKTLLPSEQRELLRSGYGVTNLVNRATAT
                     ADQLAPEDFVAGRKRLALKLRRYRPKAVAFLGVGAYRHAFGRPKATIGPQPEKFEAAA
                     VWVLPNPSGLNANYQLAELVQLFRRLI"
     gene            complement(3962..4735)
                     /locus_tag="VEL04_06455"
                     /pseudo
     CDS             complement(3962..4735)
                     /locus_tag="VEL04_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004528634.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
     gene            complement(4710..>5616)
                     /locus_tag="VEL04_06460"
     CDS             complement(4710..>5616)
                     /locus_tag="VEL04_06460"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HYT48044.1"
                     /translation="TIYTSTNQYFVIMELAPRYQTDPSVMPMLYVRSSTGALVPLNAV
                     ARLTHGVGPMQVTHLGQLPSVTFSFDLRPGVALSQAIGEVEVAMRELQVPATLTGTFS
                     GTAQAYQSSLQGMGILLLLSIVVIYLVLGILYESFIHPLTILSGLPTAGLGALITLWA
                     FREMGFNVELNMYAFVGIIMLVGIVKKNAIMMIDFAIEAQRKDGLPPEEAIHQAGLVR
                     FRPIMMTTCAALMGSLPIAIGFGAGGGARQPLGLAVVGGLLLSQVLTLYITPVIYLYF
                     ERLQSWLNRGKQPEVAPDAAHPRSA"
BASE COUNT          991 a         1808 c         1870 g          947 t
ORIGIN      
        1 ccagaagatc tggcgcggag gcaactccgc cgccggtgtg ccggcctgtg cgggctgtca
       61 cggcccggcg ggcgcgggcc tgcctgcgca gtaccccagg cttgcaggcc agtatgcgga
      121 atacgtcgcc gcacagctca aggcgttcag ggagggtagc cgcgccaatg acccgaacgg
      181 cgcgatgcgc ggcgttgcga tccgcctcag cgagcgcgag attcgcgccg tggccgagta
      241 tgctgccgga ttgcgctagg ccgggaaacg aaataaaacg ctcccccggc ctgtttattc
      301 ctctactcaa cccctaggga gaacccgatg aagaagagcc ttgtagcggc cggcgtggcc
      361 gtcgcggtgg cgagcggcgt tgcgctcgcg caggtcaagc cggagatcct ggtcaagcag
      421 cgtcaggcgg tgatgacgct gcagggcaag tacttcggcc cgatggccgg catggcgcag
      481 ggcaagctgc cgtatgacgc taacgtcgtg cagcgcaatg ccggcttcct tgacaacctg
      541 agcaggatgc cctgggacgg attcgatccg agcaccaagg acgtaagcgt aaaaaccgct
      601 gcgctgcccg cggtctggag cgacaacgcg aaattccgcg aggctgcaga ccggctgcag
      661 agcgaaacct ccaaactgta cgcggtcagc aggagcggag atgaagccgc ggtcaaggcg
      721 cagatcggcg ccgtcgggaa agcttgcggc ggctgccacg agaattttcg gcaaaagcag
      781 tagaaccgat tgatcgcagc taaaagcccc gccgcggcgg ggcttttcat tctggcgtgg
      841 tgctggagtg tggcattcgc gcagggcgac gcgaagcgcg gcgaatatat cgccaaggca
      901 gccggatgcg tcggctgcca taccgaggcg aagaagcagg ccgtgcctta cgctggcggc
      961 cgcgcgctga agacgccttt tggaacgttc tacggcccca acattacgcc gcatccgcaa
     1021 gccgggctcg ggcgctggag cgaagccgac ttcatgcgcg cgatgcgcga gggccgtcgt
     1081 cccgacggcg cgaattattt cccggctttc ccctatccgt catttacccg cataactgac
     1141 accgacctgc gcgatctgtg ggcttacctg agaacgctcg ccgcgagcag ccggcccagc
     1201 cggcagcatg agctcgggtt tttcttccgc tggcgtttcc tcgtcggaat atggaagtgg
     1261 ttctttttct cgcccggtgc tttcgcccca gacgcggcac gcagcacgat tttgaatcgt
     1321 ggcgcctacc tggtgcaggc gcttggccat tgtggtgaat gccatacgcc gcgcaactgg
     1381 ttcggggcgc cgaaagcgag ccgctatctg gcgggtgcca aaatcggcga cgcgaccgcg
     1441 tcgaacctca cccctacgcg gctgaaaaaa ttgagcgatg ccgagctgag ggaggtattg
     1501 cgcaccggcc tcacgccgga tggagatgtg ctggcggagt cgatggccga agtggtgcaa
     1561 aacaccaccg gtcaactgac ccagcaggat ctcgatgcgc tacttgcgta cctgcgcagc
     1621 ctgccgccat tgcctgacgg gcccaaatag cgtggaatag cggcgttcgc tttcctgttc
     1681 cctccatgaa cgaagccgag cgcctggttg agctgatccc gcggctgcgg cgttacgcgc
     1741 gcgcgctcgt cggcgatcgc gcctccgccg acgacctggt gcaagacacg ctggagcgtg
     1801 cctgggcaaa gctccacctc taccgccgcg gcaccgacct gcgcgcatgg ctctttaccg
     1861 tgatgcacaa cgtacacgtc aaccgcgtgc gtgccgcgcg tgccaccgat ccgctcgatg
     1921 acgagatgcc cgagctcgcg cagcgcgccg cacagggcga tgcgttgttg gtgcgcgacc
     1981 tcgaccgcgc catcgcgcgc ctgcctaccg agcagcgcgc cgtgcttctc ctcgtgacgc
     2041 tcgaggagat gagctacgag gacgtggctc gcgctctcgg cattccgatc ggcaccgtga
     2101 tgtcacgact ttcgcgtgcg cgcgagaagc tgcgtttcat gatgcttggc cagagcgcgg
     2161 cggccaagct caaggtcgtg aagtgaacga agaacagcaa ccctccgaga gcgagctgca
     2221 ggcctacgcc gacgggcgta tcgccgccga gcaaagcgcc gccatcgaat cctggcttgc
     2281 cgcgcacccc gacgacgccg agcgcatcga gacttatcgc cgcctcggcg aggacctgcg
     2341 cgcgcgctat gaccccgtgc tcgaggaggc agtgcccggc cagctgaggc gcgtcatcgg
     2401 cccgatgcgg cgctggcacc cggtcgcgaa agcggccgga tgggccacac tcggcggcgc
     2461 gctcggcgcg ctggctggct ggcagctcca cgaattgcgc cccaccgctc ccgcggccag
     2521 cgactccggt gcggtgatgg cgcggcgcgc cgcggtcgcg cacgcgacct attcgccgga
     2581 ggtgcgccac ccggtcgaag tcggcgccga gcaggagcaa cacctgatcg catggctctc
     2641 caagcgcctg ggtgcgaagg tgcaggcacc gaagctcgac gaggcgggaa tgtcgctcgt
     2701 cggcgggcgg ttgctgccgg gcgaaagcgg tccggtcgcc cagttcatgt accagaccgc
     2761 gtcggggcgc agggccacgc tatacgtgcg tgccgaggct cgcggccatc gcgagaccgc
     2821 gttccggtac gcgcgcgaga acaacgtcag cgtgttctac tggatcgacc gcgagtgcgg
     2881 ttatgcgctc gcctccgcgg acctgagcag agaagagctg ctgcgtatcg ctacgctggt
     2941 ctacaagcag ctcgagccct gaaattcacc cgggtggggc gtgtcgcccg atcgctgcac
     3001 tgctttgggg acttgaagca ccgcctcgcc gaggattagc ccaggaaacc cggtgatcgg
     3061 ggcgcagttt atccctggca tgcttgttgc gatttcgggg tatgtctttc ctggatgtcc
     3121 aggacctcgc cacagtcgcg gcgcaagtcg acattgcgcg gctgatcagc gcctggtgct
     3181 gcgaatccga tacgctgctc gctgccgtcg aggccggcct cggcgcctgt ttggtcgctt
     3241 tcgtgattag gcggaaagcg aagcgctgag agcgctcgcg agcgcggcgt cgccgctaac
     3301 cacggcctgc caggaggaga aacggtggcg atcaatgcgt cccaccggac gtgccggccc
     3361 gaaggcgagg gtcgcgatcc ggcgaatcgc tcgtccagaa tatgcagctt cgacacgagg
     3421 ttcaccgacc ctgggtcata tcagtcggcg aaatagttgg acgagctccg caagctgata
     3481 gttcgcgttg aggccgctcg gattgggcag aacccagacc gcggccgctt cgaacttctc
     3541 gggctggggg ccgatcgtcg ccttagggcg gccaaacgca tggcgatagg cgccaacacc
     3601 caggaaggcc acggctttcg ggcgatagcg ccggagctta agggcgagcc gcttgcgacc
     3661 agcaacgaaa tcctccggcg caagttgatc cgcagtggcg gtagcgcgat ttacgagatt
     3721 ggtgacgccg tatccgcttc gcagcaactc gcgttgctcc gacggcagga gtgtcttggc
     3781 tgtaaaaccg gccgcatgca gcgccggcca gaaacggtta cccgggcgcg cgaagtggta
     3841 gccggtggcg gcagagtaca gccctggatt gatgccgacg aacaacacgc gcaggccggg
     3901 cgcgattacg tccttgatgc ccttgccggc agccgccgcg agatcggctg cgctaggtcg
     3961 accggacgat gtatccgccc gatttctcgt ccggctcaag cttgatcagg cccttctcgc
     4021 cggcttcctc gagcagatcg gagaaggcgc gaaagccgta gtagttctcg ttgaacccgg
     4081 ggttcctgcg cttgagcgcc tgcttcacca tcgagcccca gacgcgctcg tcggcgccgc
     4141 gctcggcgac cagtgcttcg agcgtgccga gcagcaggtc gaacgcctcc tgcttcttgt
     4201 cctggccggc gggcgccgcc tccttccgct tcttggccgc gcggcgcttc tgctcgtctg
     4261 cgcgaaccag gtcgtcgtag tagatgaact cgtcgcagtt gttgatcagc aaatccgagc
     4321 tcgagttctt gaccccgacc ccgatcacgg tcttcgcgtt ctcgcgcagc ttcgacacca
     4381 gcggcgagaa gtccgagtcg ccgctcacga tgacgaaagt gtcgacgtgc tccttggtgt
     4441 agcacaggtc gagcgcgtcg accaccatgc ggatgtcggc cgagttcttg cccgactggc
     4501 gcaggtgcgg gatctcgatc agctcgaacg ccgcggcgtg catcgtggcc ttgaattcct
     4561 tgtagcggtc ccagtcgcaa taggccttct tcaccacgat cgaacccttg gcgagcaggc
     4621 gctcgagcac tttctccatg tcgaactgtg catacttcgc ctcgcgcacg ccgagcgcga
     4681 cgttctcgaa gtcgcagaac accgcgaggc tacgcgcttc gggggtgagc ggcatccggc
     4741 gcaacctcgg gttgtttgcc tctgttcagc cagctctgca gccgctcgaa gtagaggtag
     4801 atcacgggcg tgatgtaaag cgtgaggacc tgcgacaaca gcaacccgcc gaccaccgcg
     4861 aggccgagcg gctgacgcgc gccgccgccg gcgccgaagc cgatcgcgat cggcaggctg
     4921 cccatcagcg cggcgcacgt ggtcatcatg atcggccgga agcgcaccag gcccgcctgg
     4981 tggatcgcct cttccggcgg caggccgtcc ttgcgctgcg cctcgatcgc gaagtcgatc
     5041 atcatgatgg cgtttttctt cacgatgccg accagcatga tgatgccgac gaaggcgtac
     5101 atgttgagct cgacgttgaa gcccatttcg cggaaggccc aaagggtgat gagcgcgcca
     5161 aggccggcgg tcggcaggcc cgagaggatc gtgagcggat ggatgaagct ctcgtagaga
     5221 atgccgagca cgaggtagat caccacgatc gagagcagca gcagaatgcc catgccctgc
     5281 agcgagctct ggtaggcctg cgcggtgccc gagaacgtgc cggtgagcgt cgccggcacc
     5341 tgcagctcgc gcatcgcgac ctccacctcg ccgatcgcct gcgacagcgc gacgcccggg
     5401 cgcagatcga aggagaaggt caccgagggc agctgcccga gatgcgtgac ctgcatcggg
     5461 ccgacgccgt gggtgagcct cgccacggcg ttgagcggca ccaacgcgcc ggtcgaggag
     5521 cgcacgtaga gcataggcat caccgacggg tcggtctgat agcgcggtgc aagctccatg
     5581 atcacgaaat actggttggt cgaggtgtag atggtc
//
LOCUS       DASPXW010000283         6406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1092716, whole genome shotgun sequence.
ACCESSION   DASPXW010000283 DASPXW010000000
VERSION     DASPXW010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6406
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092716"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            699..1907
                     /locus_tag="VEL04_06465"
     CDS             699..1907
                     /locus_tag="VEL04_06465"
                     /inference="COORDINATES: protein motif:HMM:NF012730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-gated ion channel"
                     /protein_id="HYT48045.1"
                     /translation="MSLRTGVFGLLDPGSVAHAARRVRTLEVLLILIGISSVSAGTVD
                     GIAPAARAVAATITGIVAVLFFFEYLVRLWVAPEMPHLHAATEGRSRLRWVISPEGVI
                     DLLAVMPAVAAVSGGMRLGAESASVFVLLWVLKLATHAPGVEMIARVFHNERSAIAAA
                     AALFAIVLFSAATIEHWLEGERQPEAFGSIPASLWWAVVTLTTTGYGDVVPQSAPGRV
                     VGGLVMLCGIAVLALLAGILATGFAEEVRRREFVRIWDLVARVPFFSEVGAIAISDIV
                     GRLRSRSYAAGVTVIRKGEAGDSMFFIVTGEVEVRLGPRTRALRDGDFFGEMALLDRR
                     PRSADVATLTPCTVLVLNVADFYQLVGQQPALIAAIETEAKRRRADGRTADASRGLDK
                     EPDHDKNVAT"
     gene            1885..2208
                     /locus_tag="VEL04_06470"
     CDS             1885..2208
                     /locus_tag="VEL04_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48046.1"
                     /translation="MTKTLLLSLLAAVSTGAMAQRPDSGSADRWNVTGPAWYQTQCGH
                     VGFSPTHGIPYEKWVRPCSVPIRVCEFKTLTLNFPADPDVPCNEVIKPLPDGGVQKTY
                     LLRPH"
     gene            complement(2234..2839)
                     /locus_tag="VEL04_06475"
     CDS             complement(2234..2839)
                     /locus_tag="VEL04_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48047.1"
                     /translation="MIRAIAVLLIAAVSAVAVAQAPANPPVRIRGTVEKIDGQILTIK
                     TNSGQSMTVRLADSFVVMGIAKASVADIGSGKFIGTTTVGERDGALVALEVHIFPENM
                     RGTGEGHYDWDLRPASKMTNANVANVTNMGKDRVLTVQYKGGEKKVLVPENAVVVAFT
                     PAERSELKPGAKVFVNSQRQPDGSLTAPRVNVGLKGQIPPM"
     gene            complement(2916..5711)
                     /locus_tag="VEL04_06480"
     CDS             complement(2916..5711)
                     /locus_tag="VEL04_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018308772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYT48048.1"
                     /translation="MAPTVDPDKLLAVIAEVAREARPHVEAHVALDSPLERELGLDSL
                     ARVELVLRLEREFGASLPEQALAAAETPRDLLRFLLASAGQAPRAADASVASLVQSEG
                     VRPPAQAQTLTEALEYHVERQPERLTVFLYEEQKETQITYRALWEGALGYAARLAAAG
                     LQPGQPVAIMLPTSREYLYSFYGVLLAGGVPVPLYPPARLTTIEDHMTRHVGVLKSCG
                     ATLMITIPEAKALAWLLRAQVESLQMVLVPDDFKNFVDKKFSPVHGKRGDIGFLQYTS
                     GSTGNPKGVVLSHANLLVNVQAMGAAVRAGPADVFVSWLPLYHDMGLIGGCFATMFLG
                     FPVVLMSPLAFLSRPSAWMRAIHRHRGTISGGPNFSYELCLRRIADQDMEGIDLSSWR
                     FAFNGAEPVSSETILNFESRFSKWGFRKNCLSPVYGLAECTVGVAFTTPGEPWRVDLL
                     DRDAFSSTAEAVPADPGDSSPLKIVGCGQVIRDHDLRVVDAAGLELPDRQEGLLQFRG
                     ASATSGYYRNPEATKQLFSGEWVNTGDRAYLSGGMVYITGREKDIIIRGGRNISPYEL
                     EQAVGDLAGVRRGCVAVFGSLDKASGTERLVVIAEMREAGTARHDEVRRMINELAVSL
                     IGAPADDIVLAPPATVPKTSSGKIRRVAAREFYERGASSVKPHAVWLQFLRLVLAGAM
                     PQLRRGRRALLGLLFALRGYLVFGALFPFAFLAAMFAPARICWNVGRNVARLFLKLSG
                     IPLVVRGLENLPQAKAAVLAVNHTSYLDAVVLLSLLDYRGYAFVAKREFEGNFWMRTL
                     LSGFGTQFVERFDVAKSAAQANELTEAAKRGKSLIAFPEGMLKRATGLLPFRTGAFQA
                     AAQAGIAVVPVALRGVRSVLRDGTWYLRRSPVAVTVGEPIAPQGDDWNAAVRLRDRVR
                     AEILRHCGEPDLEPAAR"
     gene            5766..>6406
                     /locus_tag="VEL04_06485"
     CDS             5766..>6406
                     /locus_tag="VEL04_06485"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYT48049.1"
                     /translation="MLSWLVARFGAAPRASELVAAGKRAEREGRRGEACELYRGAIAA
                     DPLHAAGYLNLGAALEEGGELDAAEAAYRALLERDAENPYANYNVANMVFARGGDPQG
                     AAQLLRKALRAKRDFPEAQVALSNALDALGRPGEAAEQLRIALEQRPDYAGAWYNYGV
                     VLRKLERFDEAEEALRRAIEFAPDYLPAHGRLCALLRAEGRIDEALRCYHVARG"
BASE COUNT         1054 a         2106 c         2161 g         1085 t
ORIGIN      
        1 tccaaagccc gccccgctgc cggtctacca gcggcagtat tcaggggatc ggtatccgca
       61 agtggagcag cagcatgcgc ttcgcagcca acactaccgt taccagccgc gtgacaccgt
      121 agtgcggcag cactataggg agcaggcagc gcagtcagcg cgcgctcctg cagcgccgcc
      181 cgagcagcgc gcgccaatgt cgcaaggcca agaacataga ccggaaggta gggcagcctc
      241 gcaggagcca catcggggtc aggggaaagg gcagggtaaa ggtcgcggca aagatgaata
      301 gggaggtcgg gaacgcaata agtaatacga cctgagcttg gttactgact gacactatct
      361 gacggtgggc tccaggtccg gctcgccgcg acccgcagca ggcgctagcc gtacactttc
      421 cagccgaacc ccgaacactc gctgcatacc tgggttccga ctttcccggt ggcctcgcag
      481 gactcgcatt ggtcccacag ataggtcatg gcgctcttga acgtgccgta acagcgcctg
      541 ccgctgacca ccttattgca gcggtggttg agatattcgt gccggttcgt cagcgcatag
      601 cagacgctac agacacccag gggcttgggt cttttgtcgg gccgcatgac tacgtgccat
      661 tatgttgtct cagccgtgtg aattccaagg gggctgggtt gtcgctgcga acgggagtct
      721 tcgggctgct tgatccgggc agcgtcgcgc acgccgcacg ccgggtgcgc acgctcgaag
      781 tcttgctcat cctgatcggc atttcctcgg tctcggccgg aacggttgac ggcatcgcgc
      841 ccgctgcgcg cgcggttgcg gccaccataa ccggcatcgt cgctgtgctg ttttttttcg
      901 aatacctcgt gcggctctgg gtcgcgccgg aaatgcctca cctgcatgcg gctaccgagg
      961 gtcgatcgag actgcgctgg gtgatttcgc ccgaaggggt gattgacctg ctcgccgtga
     1021 tgccggcggt ggccgcggtt tcgggaggga tgcggctcgg cgccgagtcg gcgtcagtat
     1081 ttgtgctgct gtgggtgctg aagctcgcca cgcatgcgcc gggcgtcgag atgatcgcgc
     1141 gcgtgtttca caacgagcgc tcggcgattg cggccgcggc ggctcttttc gcgatcgtgc
     1201 tgttttcggc ggcgacgatc gagcactggc ttgagggcga gcgccagccg gaggctttcg
     1261 gcagcattcc ggcttcgttg tggtgggcgg tagtcacgct cacgacgacc ggctatgggg
     1321 acgtggtgcc gcagagcgcg ccgggtcgcg tagtcggcgg cttggtcatg ctgtgcggca
     1381 tagcggtcct ggcgctgctc gccggcatcc tcgcaaccgg gtttgccgag gaagtgcgcc
     1441 ggcgggaatt cgtgcgcatc tgggacctgg tcgcacgcgt gccgttcttt tctgaagtcg
     1501 gcgcaatcgc tatttccgat atcgtcgggc gcctgcgctc ccgcagctac gcggcgggcg
     1561 tgacggtgat tcgcaagggc gaagccggcg actcgatgtt cttcatcgtc acgggcgagg
     1621 ttgaggtgcg gctcggccct cgcaccagag cattgcgcga cggtgatttt ttcggcgaga
     1681 tggcgctgct cgaccgcagg ccgcgctcgg ccgatgtggc gacgctgacg ccttgcaccg
     1741 tgctggtgct taacgtagcg gatttttacc agctagtggg ccagcagccc gcgcttatcg
     1801 cggcgatcga gacggaagcg aaacgccgcc gcgcggatgg tcgtacggcg gacgcttccc
     1861 gagggctgga caaggagcct gatcatgaca aaaacgttgc tacttagttt gctggctgcc
     1921 gtcagcaccg gcgcaatggc gcagcgaccc gactccggga gcgccgaccg ctggaatgtc
     1981 actggccccg cctggtatca gacgcaatgc gggcatgtcg ggttctcgcc gacccatggg
     2041 attccatacg agaaatgggt gcgcccctgc tcggtaccga tcagggtatg cgaattcaag
     2101 accctgacgc tcaatttccc ggccgatccc gacgtccctt gcaacgaggt gatcaaaccg
     2161 ctgccggacg gcggggtgca aaaaacctac ctgcttcgcc cgcattagtt gcgccgcccg
     2221 cgcgcgcggc gcgctacatc ggcgggatct gccccttcag cccgacgttg acgcgcggcg
     2281 cggtcaggct gccgtcgggc tggcgctgcg aattcacgaa caccttggcg ccgggcttga
     2341 gctcgctgcg ctccgccggt gtgaacgcca cgacgaccgc gttctccggt accaggacct
     2401 tcttttctcc gcccttgtat tgcaccgtga gaacgcggtc cttgcccatg ttcgtcacgt
     2461 tggccacgtt cgcattggtc atcttgctgg ctggcctcag gtcccagtcg tagtgccctt
     2521 cgccggtgcc gcgcatgttc tccgggaaaa tgtgaacctc cagcgcaacc agcgcgccgt
     2581 cgcgctcgcc gacggtggtg gtgccgatga acttgccgct gccgatgtcg gcaacgctcg
     2641 ccttggcgat ccccatgacc acgaagctgt ccgccagcct gacggtcatg ctctggccgc
     2701 tgttggtctt gatggtcagg atctggccgt cgattttttc caccgtgccg cggattctca
     2761 cgggcgggtt ggctggagcc tgggcaacgg caacggcgga tacggcggca atcagcaata
     2821 ccgcgatagc acgaatcatg gggtctcctt cacggtgaac aatggaacgc aaacgtccgg
     2881 gatgcggcag ttattccctg gcctggtagg tgatactacc tcgccgcggg ctccaggtcc
     2941 ggctcgccgc agtgcctgag gatctcggcg cgcacgcggt cgcgcagccg cacggcggca
     3001 ttccagtcat cgccctgcgg cgcgatgggc tcgccgacgg tgaccgcgac gggcgagcgg
     3061 cgcagatacc aggtgccgtc gcgcagcacc gagcgcaccc cgcgcagggc caccggtacc
     3121 accgcaatgc cggcctgcgc cgccgcctgg aaagcgccgg tgcggaacgg caagagcccg
     3181 gtcgcgcgct tcagcattcc ctcggggaaa gcgatcagcg acttgccgcg cttcgcggct
     3241 tccgtcagct cgtttgcctg cgcggcgctc ttcgcgacgt cgaagcgctc gacaaactgg
     3301 gtgccgaaac ccgacagcag cgtgcgcatc cagaaattgc cctcgaactc gcgcttggcg
     3361 acgaaggcgt agccgcggta atcgagcaac gacagcagca ccaccgcgtc caggtagctg
     3421 gtgtggttca ccgccagaac cgcggctttc gcctggggaa ggttttccag cccgcgaacc
     3481 actagcggaa tgccggagag tttcagaaaa aggcgagcca catttcttcc cacgttccag
     3541 cagatgcgcg cgggcgcgaa cattgcggca aggaaagcaa agggaaacag cgcaccgaac
     3601 accaggtagc cgcgcaacgc gaacagcagg ccgaggagtg cgcgccgccc ccggcgcaac
     3661 tgcggcatgg cgccggcgag caccaggcgc aggaactgca gccacacggc gtgcggcttc
     3721 accgacgagg cgccgcgctc gtagaactcg cgagccgcga cgcgccggat cttgccgctc
     3781 gaggttttcg gcacggtagc cggcggcgca agcacgatgt cgtccgccgg cgcgccgatc
     3841 aggctgaccg cgagctcgtt gatcatgcgc ctgacttcat cgtggcgcgc agtgccggct
     3901 tcgcgcatct cggcaatcac caccagccgc tcggtgccgc tcgccttgtc gaggctgccg
     3961 aacactgcga cgcagccgcg ccgcacgccg gcgaggtcgc cgaccgcctg ctccagctcg
     4021 taggggctga tgttgcggcc gccgcggatg atgatgtcct tctcgcgtcc ggtgatgtag
     4081 accatgcccc ccgacaggta ggcgcggtcg ccggtattca cccactctcc ggagaacagc
     4141 tgcttcgttg cctcggggtt gcggtagtag cccgaggtgg ccgaagcgcc gcggaactgc
     4201 agcaagcctt cctggcggtc cggcagctcc aggcccgccg catcgaccac gcgcaggtcg
     4261 tggtcccgga tcacctggcc gcagcccacg attttcaatg gagaagaatc gccggggtcg
     4321 gcaggaaccg cctctgcggt gctcgaaaac gcatcgcgat cgagcaaatc gacgcgccac
     4381 ggctcgcccg gcgtggtgaa cgccacgccc accgtgcact cggcgaggcc gtacacggga
     4441 gacaggcagt tcttgcgaaa gccccatttg gaaaatctgc tttcaaagtt aagaattgtt
     4501 tccgagctca ccggttctgc gccgttgaag gcgaaacgcc acgaggaaag gtcgatgccc
     4561 tccatgtcct ggtctgcgat gcgccgcagg cacagctcgt aggagaagtt cgggccgccc
     4621 gatatggtgc cgcggtggcg gtggatggcg cgcatccatg cgctcggccg cgacaggaac
     4681 gccagcggcg acatcagcac cacggggaag ccgaggaaca tggtggcgaa gcagccgccg
     4741 atcagaccca tgtcgtggta gagcggaagc cagctcacga acacgtcggc ggggccggcg
     4801 cgcaccgccg cgcccatcgc ctggacgttg accagcaggt tggcgtgcga aagcaccacg
     4861 cccttcggat tgcccgtgct gcccgaggtg tactgcagga agccgatgtc accccgtttg
     4921 ccatgcacgg gagaaaattt tttatcgacg aaattcttga aatcatccgg caccaggacc
     4981 atctgcaagg actccacctg tgcgcgcagc agccaggcga gcgccttcgc ctccgggatc
     5041 gtgatcatga gcgtcgcgcc gcagctcttc aggacgccga cgtggcgcgt catgtggtcc
     5101 tcgatcgtcg tcagccgcgc cggcgggtag agcggcaccg gcacgccgcc ggcgagcagc
     5161 acgccgtaga aggagtagag gtactccctg gatgtcggca gcatgatcgc caccggctgc
     5221 ccgggttgca gcccggcggc ggcgagacgc gcggcgtaac cgagcgcgcc ttcccacagc
     5281 gcgcggtagg ttatctgagt ttccttctgc tcttcgtaca ggaaaaccgt cagtctttca
     5341 ggttgacgct cgacgtgata ctcgagcgct tcggtgagcg tctgcgcctg cgcgggcggg
     5401 cgcacgcctt ccgactgcac caggctcgcc acgcttgcgt cggcggcgcg cggcgcctgg
     5461 ccggcgctcg ccagcaggaa gcgcagcagg tcgcggggcg tctcggccgc ggcgagcgcc
     5521 tgctcgggga ggctggcgcc gaactcgcgc tccaggcgca gcaccagctc gacgcgtgcc
     5581 aggctgtcga ggccgagctc gcgctcgagc gggctgtcga gcgcgacatg cgcctcgacg
     5641 tgcggccgcg cctcgcgcgc cacctcggca atgactgcga gcagcttgtc cgggtcgacg
     5701 gtcggtgcca cgccgcgcga gtgtacactc gctgtcccgc gaatcttcgg ggagacgacg
     5761 cattcatgtt gagctggctt gttgcgcgct tcggcgccgc gccccgcgcg agcgagctgg
     5821 tcgcggccgg caagcgcgcc gaacgggagg gacgccgcgg cgaggcctgc gagctctacc
     5881 gcggcgcgat cgcggccgat ccgttgcacg ccgccggcta tctcaacctc ggcgcggcgc
     5941 tcgaagaagg cggagagctc gacgctgccg aggccgcgta ccgggcgctg ctcgagcgcg
     6001 acgcggaaaa tccctacgcc aactacaacg tcgcgaacat ggtgttcgcg cgcggtggcg
     6061 acccgcaggg cgcagcacag ttgctgcgca aggcgctccg cgcgaagcgc gactttcccg
     6121 aggcccaggt cgcgctgtcg aatgcgctcg atgccctggg ccggccgggg gaagcggcgg
     6181 agcagctgcg catcgccctc gagcagcgtc ccgactacgc cggcgcctgg tacaactacg
     6241 gcgtcgtgct gcgcaagctc gagcggttcg acgaggcaga ggaggcgctg cgccgcgcga
     6301 tcgagttcgc gcccgattac ctgccggccc acggcaggct ctgcgctctg ctgcgcgccg
     6361 aaggcaggat cgacgaagcg ttgcgctgct accacgtggc gcgcgg
//
LOCUS       DASPXW010000284         2514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1092756, whole genome shotgun sequence.
ACCESSION   DASPXW010000284 DASPXW010000000
VERSION     DASPXW010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092756"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="VEL04_06490"
     CDS             <1..684
                     /locus_tag="VEL04_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HYT48050.1"
                     /translation="RPALFTHDEVLTLFHEFGHGLHHLLTRMEDLGVSGLGGVEWDAV
                     ELPSQFMENFCWEWEVLRQMTRHVDTGSPIPKTLFDRMLAAKNFQSGLDTLRQIEFAV
                     FDMRLHSDFDPGAGHSALQLLEEVRERVAVLKPPPYNRFPNSFGHIFGGGYAAGYYSY
                     KWAEVLSADAYSFFEEQGVLSADAGRRFRDEILAVGGSRPAAESFRAFRGREPSVEAL
                     LRHSGMISA"
     gene            681..1283
                     /locus_tag="VEL04_06495"
     CDS             681..1283
                     /locus_tag="VEL04_06495"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HYT48051.1"
                     /translation="MRRAAVLLLIFFSIGASAQQLYRWTDEKGRVHVTDTPPPPSAKS
                     VQKKAAPGAAASGAAETPGNEPYALQMARKSNPVTLYTTPGCEACGEARKLLNARGVP
                     FREVSVDSEKDLDELKKVVGSNSVPSIVVGETVQKGFEDTAYNRVLDAAGYPKAGILP
                     QRRQAEPKAESQAEAKPAPEEEAPRGPYSPGAAPQRSQRK"
     gene            1294..2064
                     /gene="xth"
                     /locus_tag="VEL04_06500"
     CDS             1294..2064
                     /gene="xth"
                     /locus_tag="VEL04_06500"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HYT48052.1"
                     /translation="MSYRLATWNVNSLKVRLPHLLDWLAQAQPDVMCLQETKTEDANF
                     PLAAIQAAGYQAVFCGQKAYNGVAILARSALTEVAYGIPDFPDDPKRSIAATVGELRV
                     VCLYAPNGQALGSDKYLYKLRWYEALAAWLKSELARRPRLAVLGDLNVAPEDRDVHNP
                     KRWEGLIHVSEPERAAFRRLLELGFADAFRLFAQPEKEFSWWDYRLMAFQRGWGLRID
                     EILLSPELAAQCVACTIDKAPRARERPSDHAPVIAELG"
     gene            complement(2061..>2514)
                     /locus_tag="VEL04_06505"
     CDS             complement(2061..>2514)
                     /locus_tag="VEL04_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HYT48053.1"
                     /translation="MPQPNQYDRQRPDVRVTARELLDFQPEKPITEAGLRNNISVGIQ
                     YLGAWLAGNGCVPVFNLMEDAATAEISRSQIWQWIRSPKGVLEDGRKVTVGLFRQLLA
                     EELPKVKTYLGEMAYSAGKYEEAATLFEKLIVGDYVEFLTLPAYQQID"
BASE COUNT          434 a          808 c          836 g          436 t
ORIGIN      
        1 aggcccgcgc tcttcacgca cgacgaagtt ctgacgctgt tccacgaatt tggtcatgga
       61 ctgcatcatt tgctgacccg catggaggat ctgggtgttt ccggcctggg cggtgtcgag
      121 tgggatgcgg tcgagcttcc aagccagttc atggagaact tctgctggga atgggaggtg
      181 ctgcggcaga tgacgcgcca cgtggataca ggttctccta ttcccaaaac cttgtttgac
      241 cgcatgctcg cggcgaagaa tttccagagc gggctcgaca cgctgcggca gatcgagttc
      301 gcggttttcg acatgcggct gcattccgac ttcgaccccg gcgcgggaca cagcgccttg
      361 caactgctcg aggaagtgcg cgagcgcgtc gcggtgctca agccgccgcc gtacaaccgc
      421 tttccgaaca gcttcgggca cattttcggc ggcggctacg cggccggcta ttacagctac
      481 aagtgggcgg aagtgctgtc cgcggatgcc tatagcttct tcgaggagca aggcgttttg
      541 agcgccgacg cgggacggcg cttccgcgac gagattctcg ccgtgggcgg cagccgcccc
      601 gccgccgaat cgttccgcgc cttccgcggc cgcgagccga gcgtcgaggc gctactcagg
      661 cacagtggta tgatttccgc atgaggcgcg ccgcagtttt gcttttgatt tttttcagca
      721 tcggtgcgtc ggcgcagcag ctttaccgct ggaccgatga gaaagggcgc gtgcatgtca
      781 ccgacacgcc gccgccgcca agcgcaaaat cggtacagaa gaaagctgcc ccgggcgcag
      841 ccgcctccgg cgcggccgaa accccgggga acgagccgta cgcgctgcag atggcgcgca
      901 agagtaaccc cgtcacgctc tataccacgc cgggctgcga ggcttgcggc gaagcgcgca
      961 agctcttaaa tgcgaggggc gtgcccttca gggaagtgag cgtcgacagc gagaaggacc
     1021 tcgacgagct gaagaaagtg gtcggcagca attcggtgcc gtcgatcgtc gtcggcgaga
     1081 cggtgcagaa gggcttcgag gatacggcct acaatcgcgt gctcgacgcc gccggctatc
     1141 cgaaggccgg catcctgccg cagcgcaggc aggccgagcc gaaggctgaa tcccaggccg
     1201 aggcgaagcc ggcgccggaa gaggaagcgc cccgcgggcc ctattcgcct ggagctgcgc
     1261 cgcagcgctc gcagaggaag tgacgccgga gaagtgagct acaggctcgc gacctggaac
     1321 gtcaattcgc tcaaggtccg tctaccccac ctgctcgact ggctcgcgca agcgcagccc
     1381 gacgtgatgt gcctgcagga gaccaagacc gaggacgcga attttccgct cgccgcgatc
     1441 caggccgcag gctaccaggc ggtgttctgc ggccagaaag cctacaacgg cgtcgcgatc
     1501 cttgcgcgct cggcgctgac cgaagtcgcg tacggcatcc cggattttcc cgacgatccc
     1561 aagcgcagca tcgccgcgac cgtcggcgag ctccgcgtag tctgcctgta tgcgccgaat
     1621 ggccaggcgc tcgggtcgga caagtacctc tacaagctgc gctggtacga agcgctcgcg
     1681 gcatggctga aaagcgagct tgcgcgccgg ccgcggctcg cggtgctcgg cgatctcaac
     1741 gtcgcgccgg aggaccgcga cgtgcacaac ccgaagcgct gggaaggcct gatccatgtc
     1801 tcggagcccg agcgcgccgc cttccggcgc ctgctcgagc tcggcttcgc ggacgccttc
     1861 cgcctgttcg cgcagccgga gaaggaattc tcgtggtggg actatcgcct gatggcgttc
     1921 cagcgcggct ggggactgcg catcgacgaa atcctgctgt cgccggaact tgccgcgcaa
     1981 tgtgtcgcct gcacgataga caaggcaccg cgcgcacgcg agcggccgtc ggatcacgcc
     2041 ccggtcatcg cggagctcgg ctagtcgatc tgctggtagg ccggtagggt caggaactcg
     2101 acgtagtcgc cgacgatgag cttctcgaac agcgttgccg cttcctcgta cttgccggcg
     2161 ctgtaggcca tttctccgag gtaggtcttc actttcggca gctcttccgc cagcaactgc
     2221 ctgaagagcc cgaccgtcac cttgcggccg tcttcgagga cgcccttcgg cgagcggatc
     2281 cactgccaga tctgcgagcg cgagatctcg gcggtggcgg catcttccat caggttgaac
     2341 accggcacgc agccgttgcc ggcgagccac gctccgaggt actggatgcc gaccgagatg
     2401 ttgttgcgca gcccggcctc ggtgatcggc ttctcgggct ggaaatcgag cagctccctg
     2461 gcggtgacgc gcacgtccgg gcgctggcgg tcgtactggt tcggctgcgg catg
//
LOCUS       DASPXW010000285         4096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_963235, whole genome shotgun sequence.
ACCESSION   DASPXW010000285 DASPXW010000000
VERSION     DASPXW010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963235"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1309)
                     /gene="dsbD"
                     /locus_tag="VEL04_06510"
     CDS             complement(<1..1309)
                     /gene="dsbD"
                     /locus_tag="VEL04_06510"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF001419.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HYT48054.1"
                     /translation="MKLFALLAALLVATPASGANDLLEPEKAFRFSARALDASAIEVS
                     FAIADGYYLYRERFKFTLQDTPGARLGRPELPRGIPHQDEFFGEVETYRKQVRMRVPV
                     EGGEGKTVKLAVTSQGCADVGVCYVPMESMASVRLAAAPGADLGGLSDAPRQVDAVPR
                     LSIFASDIDIARLFEGSAALVLGGFLLFGLLLAFTPCVLPMIPILSGIIAGEGQALSK
                     SRALALSLSYVIGMALAYALAGVAAAYSGSLLAAALQNAWVLATFALVFVLLALSMFG
                     LYELRLPGFIHQRLDQAQQRFPGGRVASVAGMGALSAVIVSPCVAAPLAGALLYIAQT
                     RDVALGGAALFAMALGMGVPLVAVGVSEGALLPKAGAWMSGVKRFFGVLLLAVALWIV
                     SPVLPAALRMIGWAALLIGSGVFLRAIDPLPVAAAPWWRLWKAA"
     gene            complement(1306..1623)
                     /gene="cutA"
                     /locus_tag="VEL04_06515"
     CDS             complement(1306..1623)
                     /gene="cutA"
                     /locus_tag="VEL04_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HYT48055.1"
                     /translation="MSALLVLTNVPERAAAERLADLLVEKKLAACVNILAPCRSVYRW
                     QGAVQHDEEHPMLIKTTSERYAALEQALRAGHPYELPEIIAVPIERGLPAYLAWVAAE
                     TAT"
     gene            1694..2749
                     /locus_tag="VEL04_06520"
     CDS             1694..2749
                     /locus_tag="VEL04_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HYT48056.1"
                     /translation="MVPSIRTVSIQPSGQQFEVEEGETVLAAALRQGLVLPYSCQSGA
                     CGTCKGKIVSGSVDYGVYQPRVFSEAEKAQGKALFCQAKPLSDLVIEARTISVAKGIQ
                     IKRLPCRVQKIERPTDDVAILYLKLPANERLQFLAGQYIEFLLKDRDKRSFSMANPPH
                     DAELLQLHVRRVAGGQFTAHVFEKMKERDILRLEGPYGTFFLREDSDKPMVFVASGTG
                     FAPIKAILEDAFEKGVTRPMVLYWGGRRPKDLYMNHLPAKWAAERPGFRYVPVISDGL
                     PEDAWTGRAGFVHRAVMEDFPDLSAHQVYACGVPIMVDSAKRDFTTLCRLPEDEFYAD
                     SFTTQADLAQPPAASPR"
     gene            complement(2722..3426)
                     /locus_tag="VEL04_06525"
     CDS             complement(2722..3426)
                     /locus_tag="VEL04_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT48057.1"
                     /translation="MLLEVKGLEVNYGGIRAVKGIDLDITAGELVCLIGANGAGKTST
                     LKAICRLIPSRSKCMRYAGAEIAAAPVHELPRRGLVMVPEGRGIFPQLTVEENLAMGA
                     YARGGGEPESQYEAFPRLKERRRQVAGTLSGGEQQMLAIARALMACPKLLLLDEPSMG
                     LAPLMVAKIFEIVREIAQRGVTILLVEQNARLALELAGRGYVMESGAVTLAGDARRLL
                     EEPKVREAYLGEAAGG"
     gene            complement(3416..>4096)
                     /locus_tag="VEL04_06530"
     CDS             complement(3416..>4096)
                     /locus_tag="VEL04_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT48058.1"
                     /translation="QILGLIGPNGAGKTTLFNVLTGNYAPDAGSFVFDARNVTALKPH
                     QIAASGIARTFQNIRLFANLSTLENVMIGRHVRTRAGVWGAILRNRRTVAEEIAIERR
                     ALELLDYVGIRSRANHPAGGLPYGDQRRLEIARALATEPRLLALDEPAAGMNAAESAA
                     LRRLIDSIRQGGVTVLLIEHDVRLVMNVCDRVMVLDYGEKIAEGTPLEVQRDPKVIEA
                     YLGTPRAA"
BASE COUNT          721 a         1370 c         1353 g          652 t
ORIGIN      
        1 ccgcggcctt ccacagccgc caccagggcg ctgccgccac cggcagcgga tcgatcgcgc
       61 gcaggaacac ccctgagccg atgagcagcg ccgcccaccc gatcatccgc agtgcggccg
      121 ggagcaccgg cgagacgatc cagagcgcga ccgcgagcag cagcacgccg aaaaacctct
      181 tcacgccgct catccacgcg ccggccttgg ggagcagagc cccctcggag accccaaccg
      241 ccaccagggg cacgcccatg ccgagcgcca ttgcgaacag cgccgcgccg ccgagcgcca
      301 cgtcgcgcgt ctgcgcgatg tagagaagcg cgccggcgag gggcgccgcg acgcagggac
      361 tgacgatcac tgccgagagc gcccccatcc cggcgaccga cgcgacgcgc ccgccaggaa
      421 agcgctgctg cgcctggtcc aaccgctggt ggatgaaccc cggcagccgt agctcgtaca
      481 gaccgaacat cgacagcgcg agcagcacga aaaccagcgc gaacgtcgcc agcacccatg
      541 cgttttgcag cgcggcggca agcagcgatc cggagtacgc ggccgcgacg ccggcgagcg
      601 cataggcgag cgccatgccg atcacatagg agagcgaaag cgcgagcgcg cgcgacttgc
      661 tgagcgcctg gccttctccg gcgattattc cggacaagat cgggatcatc ggcaggacgc
      721 atggcgtgaa agcgagaagc agcccgaata gcaggaagcc gcccagcacc agcgcagcgc
      781 tgccctcaaa caggcgcgcg atatcgatat cgctggcgaa gatggagagg cgcggcacgg
      841 cgtcgacctg tcgtggagcg tcgctaaggc cgccgagatc ggccccaggc gctgcagcga
      901 ggcgaacgga ggccatggat tccatcggca cgtagcacac gccgacgtcg gcgcagccct
      961 gcgaggtcac ggcgagcttg acggtttttc cttccccgcc ttccaccgga acgcgcatcc
     1021 tcacctgctt gcgataggtt tcgacctcac cgaagaactc gtcctggtgg ggaatgccgc
     1081 gcggcagttc gggcctaccc agccgagcgc cgggcgtgtc ttgcagcgtg aacttgaatc
     1141 gctcgcggta caggtagtag ccatcggcga tagcgaaact cacttcgatc gccgaagcgt
     1201 caagcgcgcg cgccgagaat ctgaaggctt tctcgggctc gagcaggtcg ttcgcgccgc
     1261 tcgccggggt ggcgacgagg agtgctgcaa ggagggcgaa aagcttcaag tcgcggtttc
     1321 cgcggcaacc caggcgagat aagcgggcag tccgcgttcg attgggaccg cgatgatctc
     1381 ggggagttca tagggatgcc ccgctcgcag cgcctgctcg agcgccgcgt agcgctcgct
     1441 ggtggtcttg atgagcatgg ggtgctcctc gtcgtgctgc acggcgcctt gccagcggta
     1501 gaccgagcgg cagggtgcaa ggatgttgac gcatgccgca agcttctttt ccaccagcaa
     1561 gtcggcgagc cgctcggcgg cggcgcgctc cgggacgtta gtcaacacca gcagcgcgct
     1621 catgtcccgg tccctcgctg acgtagacta cgcgtgcgat caagagtcgc ttcatgcgac
     1681 ctattttagc gacatggtac cgagcattcg gacagtcagc atccagccta gcggtcagca
     1741 attcgaggtc gaggagggcg aaacggtcct cgcggccgcc ctgcgccagg gtctcgtcct
     1801 gccctacagc tgccagagcg gcgcctgcgg cacctgcaag ggcaaaatcg tctccggcag
     1861 cgtggactac ggtgtctatc agccccgggt gttcagcgag gcggagaaag cccagggcaa
     1921 ggcacttttc tgccaggcga aacctctttc agacctggtg atcgaggcgc gcaccatcag
     1981 cgtggcgaag ggcattcaga tcaaacggct gccgtgccgg gtgcaaaaga tagaacgacc
     2041 caccgacgac gttgcaatcc tgtatctcaa gcttccggca aacgagcggc tgcagttcct
     2101 ggccgggcag tacatcgagt tcctgctgaa ggaccgcgac aagcgcagct tctcgatggc
     2161 gaatccgcct cacgacgccg agctcctgca actgcacgtg aggcgcgtgg ctggcggcca
     2221 gttcaccgcg cacgtgttcg agaagatgaa ggagcgcgac atcctgcgcc tcgaggggcc
     2281 ctacggcacg tttttcctcc gggaagactc ggacaagccg atggtgtttg tggcttcggg
     2341 gaccggcttc gctcccatca aggcgatcct ggaggacgcc ttcgagaaag gcgtcacgcg
     2401 cccgatggtg ctttactggg gcggacggcg tccgaaagat ctttacatga accacctgcc
     2461 ggcgaagtgg gcagccgagc gccccggttt caggtacgtg ccggtgatct ccgatggcct
     2521 gccggaggat gcctggaccg ggcgcgccgg cttcgttcac cgcgcggtga tggaagattt
     2581 tcccgatctc tctgcgcatc aggtctatgc ctgcggcgtg ccgatcatgg tggattcggc
     2641 caaacgagac ttcaccactt tgtgcaggct gccggaagac gagttctacg ccgacagttt
     2701 caccacccag gccgacctcg ctcagccgcc ggcggcttca cccaggtagg cttcgcggac
     2761 cttgggctcc tcgagcaggc gcctggcgtc gccggcaagc gttaccgcgc ccgactccat
     2821 cacgtagccg cggccggcga gctcgagcgc gagccgcgca ttctgttcga ccagcaggat
     2881 agttacgccc cgctgcgcga tctcgcgcac gatctcgaag atcttggcca ccatcagcgg
     2941 cgcgagcccc atggagggtt cgtcgagcag cagcagcttg gggcacgcca tcagggcgcg
     3001 cgcgatggcg agcatctgct gctcgccgcc cgagagagtg cccgcgacct ggcggcgtcg
     3061 ctccttgagc cgtggaaagg cctcgtactg cgactcgggc tcgccgccgc cgcgcgcgta
     3121 ggcacccatg gccaggttct cctcgacggt gagctgcgga aaaatgccgc ggccctcggg
     3181 caccatcacc agcccgcggc gcggcagctc gtgcaccggc gcggcggcga tttcggcccc
     3241 tgcatagcgc atgcatttcg aacgcgatgg aatcaggcgg cagatcgcct tcagcgtcga
     3301 ggtcttgccg gcgccgttgg cgccgatcag gcagaccagc tcgcccgcgg tgatgtcgag
     3361 gtcgatgccc ttgacggcgc gaatgccgcc gtagttcacc tccagcccct tcacttcaag
     3421 cagcacgcgg cgtcccgagg taggcctcga tcactttggg gtcgcgctgc acctcgagcg
     3481 gcgtgccttc cgcgattttc tcgccgtagt cgagcaccat gacgcggtcg cagacgttca
     3541 ttaccagccg cacgtcgtgc tcgatcagca gcacggtcac gccgccctgg cgtatcgagt
     3601 cgatcaacct gcgcagcgcc gccgattccg cggcattcat tcctgcggct ggctcgtcga
     3661 gcgcgagcaa gcggggctcg gtggcgagcg cccttgcgat ttcgaggcgc cgctggtcgc
     3721 cgtagggcag gccgcccgcc ggatggttgg cgcggctgcg gattccgacg tagtcgagca
     3781 gctcgagagc gcggcgctcg atggcgattt cttccgcaac ggtgcggcga ttgcgcagga
     3841 tcgctcccca cacgcccgcg cgcgtcctga cgtgcctgcc gatcatcacg ttctcgagcg
     3901 tggacaggtt ggcgaacaga cggatgttct gaaaggtacg cgcgatcccg ctggccgcaa
     3961 tctggtgggg cttgagcgcc gtgacgttgc gcgcgtcgaa cacgaacgac cctgcatcgg
     4021 gcgcgtaatt tccggtgagc acgttgaaga gcgtcgtctt gccggcgcca ttcggcccga
     4081 tcaggccgag gatctg
//
LOCUS       DASPXW010000286         2794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1469495, whole genome shotgun sequence.
ACCESSION   DASPXW010000286 DASPXW010000000
VERSION     DASPXW010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1469495"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="VEL04_06535"
     CDS             <1..446
                     /locus_tag="VEL04_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HYT48059.1"
                     /translation="EPGSVVEDVQRFHALGVEHVALGDTIGTATPAAVKAMFARIKGG
                     TPIAHFHDTRGTGLVNYVAAYEAGVRHFDSSFGGVGGHPAKVKYGGGHTGNVSTEDLV
                     NLFESMGIATGIDLDGLLATAELCEQALGRELYGRVTRSGLNPLL"
     gene            455..727
                     /locus_tag="VEL04_06540"
     CDS             455..727
                     /locus_tag="VEL04_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48060.1"
                     /translation="MHVHSLRLKDGHEALVARVVTQEGKTGFGFSFRLDATEARHMAE
                     WNAGVRPERPAYESQLDHPWERAWLSDEEIEWQSEAAFAMIRWLPE"
     gene            739..1431
                     /locus_tag="VEL04_06545"
     CDS             739..1431
                     /locus_tag="VEL04_06545"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HYT48061.1"
                     /translation="MGKTISFKRPDGKSVNGYLAEPAQGAKAPAIVVIQEWWGLNDQI
                     RGVADKLAAAGYRALVPDLYRGKVAVEAKEAEHLMTGLNFGDAAGQDVRGAAQHLKAS
                     GSAKVGVTGFCMGGALTLLAAVNVPEANAAVVWYGYPPLEYVDATKIKAPLQGHFATE
                     DQAFPIAKVDELEKKLKAASVKYEFYRYKAKHAFANETANERKLAMLEYNPQAAELAW
                     KRTMDFLARNLK"
     gene            complement(1432..2391)
                     /locus_tag="VEL04_06550"
     CDS             complement(1432..2391)
                     /locus_tag="VEL04_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYT48062.1"
                     /translation="MMPIRKLLCVFFIWPAFALAQSYPWKPIRVVLPFGPGGVADITT
                     RTLAPRLGEGLGQQVIVENRPGAGGVLAAQDVARAEPDGHTLLLLTNGNAVSQALFKS
                     LPYDPVNDFAMISTVGFFSLVIVTGADSQAKTLQEAIAMAKANPGKLNIGSISPGGTQ
                     HLAAELFRSTAGIDALVVPHKTTGEVIIAARNGNVDLAVDFIAPLLSSIRSGALRALA
                     VTAAKRFPDLPEVPTVIESGVAGYDVASWNALAAPAKTPRAAIERLHQELVKAIAAPQ
                     VQKRFAELGVDGRTSTPAELREFFVAESKRWSRVVEAAKIPKQ"
     gene            complement(2388..>2794)
                     /locus_tag="VEL04_06555"
     CDS             complement(2388..>2794)
                     /locus_tag="VEL04_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HYT48063.1"
                     /translation="AEFRDPPNAQRAPAGKEMMAMLRSGELDAAIVGAMPKEDNLKPL
                     IRDAEAAGRQWGEKHGAIKLNHLVGVKHTVSRLAAEEIYGLLVESKQAAGNPPALPHG
                     LDANRRNLEIAIECAYKQKMIPRRFTVEELFE"
BASE COUNT          484 a          911 c          950 g          449 t
ORIGIN      
        1 tggaaccggg ctccgtggtc gaagacgtgc agcgtttcca tgcgctcggc gtcgagcacg
       61 tcgcgctggg cgacaccatt ggaaccgcaa ccccggcggc cgtgaaggcc atgtttgcaa
      121 ggatcaaagg cggaacgccg atcgcccact tccacgacac gcgcggcacg gggctggtga
      181 attacgtcgc ggcctatgag gccggcgtgc gccacttcga ctcgtctttc ggcggcgtcg
      241 gcggccatcc ggcgaaggtg aaatacggcg gcggccacac cggcaacgtg agcaccgagg
      301 acctggtgaa tctcttcgag tcgatgggca tcgccaccgg catcgacctc gacgggctgc
      361 tcgccaccgc ggagctctgc gagcaggcgc tcggccgcga gctctatggc cgcgtcacgc
      421 gaagcgggct caacccattg ctctagctcc ctacgtgcac gttcattcgc tgcgcctgaa
      481 ggacggccac gaggcgctgg tcgcgcgcgt ggtgacgcag gagggaaaaa ccggcttcgg
      541 cttcagcttc aggctcgatg cgaccgaggc gcgccacatg gcggaatgga acgcgggagt
      601 gcggccggag cgccccgctt acgagagcca gctcgaccat ccgtgggagc gtgcctggct
      661 ttcggacgag gagatcgagt ggcaaagcga agccgccttt gcgatgatcc gctggttgcc
      721 cgaatagagg agaaggccat gggaaagacg atcagcttca agcggccgga cggaaagagc
      781 gtcaacggat atctcgccga gcctgcgcag ggcgccaaag ctccggcgat agtggtgatc
      841 caggaatggt ggggcctcaa cgaccagatc cggggcgtgg cggacaagct cgccgccgcg
      901 ggctaccgcg cgctggtgcc cgacctctac cgcggcaagg tcgccgtcga ggcgaaggaa
      961 gccgagcacc tcatgaccgg cctcaacttc ggcgacgcgg ccggccagga cgtgcgcggc
     1021 gcggcgcagc acctcaaggc gagcggcagc gccaaggtcg gcgtcaccgg cttctgcatg
     1081 ggcggcgcgc tcaccctgct cgcggcggtc aatgtccccg aggcgaacgc cgctgtcgtc
     1141 tggtacggct atccgccgct cgaatacgtc gacgcgacga aaatcaaggc gccgctgcag
     1201 ggccacttcg ccaccgagga ccaggctttc cccatcgcca aggtcgacga gctggagaag
     1261 aagctcaagg cggcgagcgt gaaatacgag ttctaccgct acaaggcgaa acacgcgttc
     1321 gccaacgaga cggcgaacga gcgcaagctc gccatgctgg agtacaaccc ccaggccgcc
     1381 gagctggcgt ggaagcgaac catggacttc ctcgccagga acttgaagta gttactgctt
     1441 cgggatcttc gccgcctcga ccacccggct ccagcgcttc gactcggcta cgaagaactc
     1501 gcgcagctcc gcgggagtgc tggtgcgccc gtcgacgcca agttcggcga agcgcttttg
     1561 cacctggggg gcagcgatcg ccttgaccag ctcctggtgc agccgctcga tggcggcgcg
     1621 cggcgtcttc gccggcgccg caagcgcgtt ccacgaagcc acatcgtagc cggcgacgcc
     1681 gctctcgatc acggttggaa cctcgggcag gtcggggaaa cgcttcgccg cggtaacggc
     1741 gagcgcacgc aacgcgcccg agcggatgct ggacagcagc ggagcgatga aatcgaccgc
     1801 cagatcgacg ttgccgttgc gggcggcgat gatcacttcg ccggtggtct tgtgcggaac
     1861 caccagcgcg tcgatgccgg ctgtcgagcg gaaaagctcc gcagcaaggt gctgcgtgcc
     1921 acccggactg atgctgccga tgttgagctt gccggggttc gccttcgcca tcgcgatcgc
     1981 ttcctgcagg gtcttcgcct gcgaatccgc gccggtgacg atcaccaggc tgaagaagcc
     2041 gacggtggaa atcatggcga aatcattcac cgggtcgtag ggcagcgact tgaacagggc
     2101 ctggctgact gcgttgccgt tggtgagaag cagcagggtg tgcccgtcgg gctcggcgcg
     2161 ggcaacgtcc tgagccgcga gcacgccccc cgcgcccggc ctgttctcca cgatgacctg
     2221 ctggccgagc ccctcgccga gcctgggcgc gagggtgcgg gtggtgatgt cggcgacgcc
     2281 tcccggcccg aagggcagga cgacccttat cggcttccac ggatacgact gggcgagcgc
     2341 gaacgcgggc cagatgaaga atacgcaaag tagttttctg attggcatca ttcgaacagc
     2401 tcctccacgg tgaatcgccg cgggatcatt ttctgcttgt aggcgcactc gatcgcgatc
     2461 tccaggttgc ggcggttggc gtcgagtccg tgcggcagcg ccggcggatt gccggcggct
     2521 tgcttgctct cgaccaggag gccgtagatc tcctcggcgg cgagccttga cacggtgtgc
     2581 ttcacgccca ccaggtggtt gagcttgatc gcgccgtgct tctcgcccca ttgccttccc
     2641 gcggcctcgg catcgcggat cagcggcttg aggttatctt ccttcggcat cgctccgacg
     2701 atcgccgcgt cgagctctcc ggaccgcagc atcgccatca tctccttgcc ggcgggagca
     2761 cgctgcgcgt tcggcgggtc gcggaactcg gcga
//
LOCUS       DASPXW010000287         2115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1346537, whole genome shotgun sequence.
ACCESSION   DASPXW010000287 DASPXW010000000
VERSION     DASPXW010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346537"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..1054
                     /locus_tag="VEL04_06560"
     CDS             167..1054
                     /locus_tag="VEL04_06560"
                     /inference="COORDINATES: protein motif:HMM:NF021357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HYT48064.1"
                     /translation="MKEGRWLLLIHQLPPKPDYFRVKIWRRLQGLGAVGIKNSVYALP
                     FSAEASEDFQWLRKEITAGGGEASVCRATFLDGLTDPQVEGMFRTARDAEYADWTRAA
                     EKVESMAEIARLERRLRDITALDHFGAAGRKAAEAALAGLKTRNVSRGKREKVPMKQT
                     WVTRPDVHVDRIASAWLIKRFIDPKARFRFGETREGAVSFDTFEADYTHEGDRCTFET
                     LLQRFGLEQDGALRAIAEMVHDLDLKDEKFGRDETPGFERLIAGIVKRHARDEARIER
                     GSELLNDLYESIQQSPPAR"
     gene            complement(1008..1631)
                     /locus_tag="VEL04_06565"
     CDS             complement(1008..1631)
                     /locus_tag="VEL04_06565"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HYT48065.1"
                     /translation="MQRRDVTLGVVAILVATVCVLLGIWQLNRLAQRRARNGVLAARL
                     ALPPLQVQDGITADSVRQRRVVAQGVYDFSAERTWPGRSFEGTPGVALITPLRLAGGA
                     AVLVDRGWVPSPDAFHVDQALYREPDTVTVTGIALIPPRGRGDVDVKGFVPFVIQLET
                     PEPARGLPRRWPPPAFDNGPHLSYAIQWFSFAVIALVGTAVLIRKGR"
     gene            complement(1613..1852)
                     /locus_tag="VEL04_06570"
     CDS             complement(1613..1852)
                     /locus_tag="VEL04_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HYT48066.1"
                     /translation="MVYLLRCRDGSLYTGITNDMQQRLASHRSGKASAYTRARRPLSL
                     VYQEFVADRGSALKREAAIRRLSRVDKLALCSAGT"
     gene            complement(1870..>2115)
                     /locus_tag="VEL04_06575"
     CDS             complement(1870..>2115)
                     /locus_tag="VEL04_06575"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HYT48067.1"
                     /translation="LSSALEKAGHPATRIETRAAVTLEVVDGAPKVTRSVLDVRGKVE
                     GIDQAGFQQAAEGAKKNCPVSKALQGNVEITMQAKLV"
BASE COUNT          390 a          694 c          690 g          341 t
ORIGIN      
        1 tggagccgct cgcgcgggca cgccaacatc tgagcgagcc ggaaattgcc gccgtcacgg
       61 ccgcggtcgc cgcgcagcat ttcttcaatc cgatcaccgg tgcactcggc gccgatgtgg
      121 ctccctgggg tgcgccgatc ggctcgtcga ttcggaatct ctggctatga aggaaggccg
      181 ctggttgttg ctgatccatc agctcccgcc gaagcccgat tacttccgtg tcaaaatctg
      241 gcggcgcctg cagggactcg gcgcggtcgg gatcaagaat tcggtctatg ccctgccctt
      301 cagcgccgag gcgagcgaag acttccagtg gctgcgcaag gagatcacgg ccggcggcgg
      361 cgaagcatca gtctgccggg cgacgttcct cgatggactc acggatccgc aagtcgaggg
      421 gatgttccgg actgcgcgtg acgccgaata cgcggattgg actcgcgccg ccgagaaggt
      481 cgaatccatg gcggagatcg cgcgactcga gcgccgcctg cgcgacatca cggcactcga
      541 ccatttcggc gctgcgggac gcaaggcggc ggaggctgca ctcgccgggc tcaagactcg
      601 aaacgtcagc cgcggaaaac gagagaaggt gcctatgaaa cagacctggg taacgcggcc
      661 ggacgtgcat gtcgaccgta ttgcgagcgc ttggctgatc aagcgcttca tcgatccgaa
      721 agctcgcttt cgcttcggcg agactcggga gggagccgtc agcttcgaca cgttcgaggc
      781 ggactacacg cacgagggcg accgctgcac gttcgaaaca ctgctccagc gcttcggcct
      841 ggaacaggac ggcgcactcc gcgccatcgc cgagatggtg cacgatctgg acctgaagga
      901 cgagaagttc gggcgggacg agacgccggg cttcgagcgg ctgatcgccg gcatcgtcaa
      961 gcgccatgcg cgcgacgagg ccagaatcga gcgcgggtct gagctgctta acgaccttta
     1021 cgaatcaata cagcagtccc caccagcgcg atgaccgcaa agctgaacca ttggatcgcg
     1081 tacgacagat gcgggccgtt gtcgaacgcg ggtgggggcc agcgccgcgg cagtcccctt
     1141 gcaggttctg gcgtctcgag ctgaatgacg aacgggacga aacccttcac atcgacgtcg
     1201 ccgcggccgc ggggtggaat caacgcaatt ccagtaacgg tcaccgtatc gggctctcga
     1261 tagagcgcct gatcgacatg aaatgcgtcg ggcgagggga cccagcctcg atcaaccagc
     1321 accgctgccc cgccggcgag gcgcagcggc gtgatgagcg ccacgcccgg cgttccctca
     1381 aaactccgtc ccggccaggt gcgctcggcc gagaaatcgt agacaccctg cgcgaccacg
     1441 cggcgctggc ggacgctatc cgccgtgatg ccgtcctgca cttgaagcgg cggcaaggct
     1501 aaccgcgccg cgagaacacc gttgcgggcg cggcgctgcg cgagccgatt gagctgccag
     1561 atgcccaaca gcacgcacac cgtcgccacc agaatcgcaa cgactcccag cgttacgtcc
     1621 ctgcgctgca caacgccagc ttatcgacgc gcgagaggcg gcggatcgcg gcctcgcgct
     1681 tcaacgcgct gccacgatcg gccacgaatt cctgatagac cagtgacaac ggacgccgcg
     1741 cgcgcgtata ggcgctcgcc ttgccgctgc gatgcgacgc aaggcgctgc tgcatgtcgt
     1801 tggtgattcc ggtgtacagg gagccgtcgc ggcagcgcag gaggtacacc atccagcgct
     1861 tgcgcggtgt cagacgagct ttgcctgcat cgtaatctcg acgttgccct gcagggcttt
     1921 cgagacgggg caattcttct tcgcaccctc ggcggcctgc tggaaccccg cctgatcgat
     1981 tccctcgact ttcccgcgca catcgagcac gctgcgcgtg accttgggcg cgccgtctac
     2041 cacctcgagc gtgaccgctg cgcgcgtttc gatacgcgtc gccgggtggc ccgccttttc
     2101 gagagcggag gagag
//
LOCUS       DASPXW010000288         4539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_837292, whole genome shotgun sequence.
ACCESSION   DASPXW010000288 DASPXW010000000
VERSION     DASPXW010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837292"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VEL04_06580"
     CDS             <1..206
                     /locus_tag="VEL04_06580"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HYT48068.1"
                     /translation="AFTAAAALLKPLGFTLSFALLTFFIVLVIFRRPLATSLLTAVGL
                     AAAFHLVFAVALDVALPVGFLGF"
     gene            208..1722
                     /locus_tag="VEL04_06585"
     CDS             208..1722
                     /locus_tag="VEL04_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HYT48069.1"
                     /translation="MDVASGLLHGFAVALQPLNILWCFVGCFLGTVVGILPGLGPAAT
                     IAMLLPLTYTMDPAGGIIMLAGIYYGAKYGGSTTSILLNMPGEPSSVVSCIDGYQMAR
                     KGRAGAALGIAAIASFVAGTFGVVALSLIAPPVAAFALSFSSPEYFALMCLGLSLVVL
                     LAGSSLIKALLSMLLGLWLASIGTDLFTAQSRFTFGQASLLGGVDFMLVAIGVFAIAE
                     VLSSIEAGEEAKLLPVPKGIRNLLPNMAELKACRFAFLNGSVTGFLLGVLPGAGATIA
                     SFISYGIEKAVSRHPEKFGHGAPEGLAAPEGANNADTGGALVPLLTLGIPGGSSTAIL
                     LSALILWGVRPGPLMMQESPDVFWGLVASMYIGNAILLLLNLPLVPLFAQILRVPVYI
                     LYPGILGISVVGAYSVSGRLFDIWLLAGFGLLGWLMGKLKYPAAPLILGFVLGSAMER
                     ALRQSLTMSQGDLGLFLTRPLSATMLALAALLLLMPLFGQFNRWRLRAVEMDDS"
     gene            1820..2788
                     /locus_tag="VEL04_06590"
     CDS             1820..2788
                     /locus_tag="VEL04_06590"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYT48070.1"
                     /translation="MMTRVVLAALALLAALAPAPAQYKPTRPVEFVVHNGPGSGNDVF
                     ARALIQVIEQEKLMPVRMQVANKVGGGSTTAASYVTEKAGDPNVIAVFVNIWLVDPLV
                     QTEAKTRLLDMTPIALVQIEPALVVTRADSPLKTLGEFVAAAKEKPGQMKQSGGSITS
                     RENVVRQLLMNKSGARWSFISFPGGGERLAALLGGHVDMMILDPSEAREQVRSGKLRV
                     LAQVAEKRLPDFQDVPTLKEAGFDVPDVPQIRGVVGPPKMPPDAVAFYASLFQKTTRT
                     PAWQRYLKDNQFQDAFLSPEDTRKFIAQFEERLRGLLPQAGVKVVR"
     gene            2819..2971
                     /locus_tag="VEL04_06595"
     CDS             2819..2971
                     /locus_tag="VEL04_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48071.1"
                     /translation="MDDIEEDWLRSEVVPTFDRIMRGEEKLIPASEVFADIELRYNAR
                     KAQNSV"
     gene            3071..3616
                     /locus_tag="VEL04_06600"
     CDS             3071..3616
                     /locus_tag="VEL04_06600"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HYT48072.1"
                     /translation="MARISLIEEQDHPELAPLIGRIKAGRRGGLLNVYKLLLHSPPLA
                     ETWLDHVGAVRWKTALTGRLREFVVIRIAYVNGIDYVINQHVPALAVAEGVTLAECEA
                     LKDWRKSSLFDAAERAALAYADAMVLATSVPDDVFENLRRHYDERAIVELSVLIGTYL
                     MHNRVMKALAIDLEADSERGR"
     gene            3724..>4539
                     /locus_tag="VEL04_06605"
     CDS             3724..>4539
                     /locus_tag="VEL04_06605"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYT48073.1"
                     /translation="MRSLALVLFALSLTVTGASAQNDANWPTKPIRVVVPFPAGSFAD
                     ITTRILQQKLGPRLGQQLVIDNRSGASGNIGADVLAKSAPDGYNFGIATNSTHAVAKA
                     LNPNLPYDPIKDFALVSLIGDAPYVLITAPDLAANSVQELIALAKSKPGALNYASAGP
                     ASLAHLAGALFAQMSGTQITEVPYRSSAQSVLDTSEGRIEMQFGTMAPVLPHIRAGRV
                     KALGVTSRKRAEVLPDVPTIDESGLPGYEASLWLAVVAPASTPPAIVARMAREL"
BASE COUNT          696 a         1540 c         1507 g          796 t
ORIGIN      
        1 cggccttcac ggccgcggcc gcgctgctga aaccgctcgg cttcacgcta agcttcgcgc
       61 tgctcacttt cttcatcgtg ctcgtgatct tccgccgtcc cctggcgacc tcgctcctga
      121 cggccgttgg cctcgcggcc gcgttccatc tcgtcttcgc ggtcgcgctc gacgtcgcgc
      181 tgcccgttgg cttcctcggc ttctgacatg gacgtagcga gcggcctcct tcacggcttc
      241 gcggtcgcgc ttcagccgct caatattctg tggtgcttcg tcggctgctt tctcggcacc
      301 gtggtcggca tcctgccggg cctcggcccg gccgccacca tcgcgatgct gctgccgctc
      361 acctacacga tggatccggc gggcggcatc atcatgctgg ccggcatcta ttacggcgcc
      421 aaatacggcg gctcgaccac ctcgatcctg ctcaacatgc cgggcgagcc atcctcggtc
      481 gtgagctgca tcgacggcta ccagatggcg cgcaagggcc gcgccggcgc ggccctcggc
      541 atcgcggcga tcgcctcgtt cgtggccggc accttcggcg tggtcgcgct ctcgctgatc
      601 gcgccgccgg tcgccgcctt cgcgctgtcg ttctcgtcgc cggaatattt cgcgctgatg
      661 tgcctcggcc tctcgctggt ggtgctgctc gccggctcgt cgctgatcaa ggcgctgctc
      721 tcgatgctgc tcgggctgtg gctcgccagc atcggcaccg atctcttcac cgcgcagtcg
      781 cgcttcacct tcggccaggc ctcgctgctc ggcggcgtcg atttcatgct ggtggcgatc
      841 ggcgtgttcg cgatcgccga ggtgctgtcg agcatcgagg cgggcgagga ggcgaagctg
      901 ctgccggtgc cgaagggcat ccgcaatctg ctgcccaaca tggcggagct gaaagcctgc
      961 cgcttcgcgt tcctcaacgg ctcggtgacc ggcttcctgc tcggcgtgct gcccggcgcg
     1021 ggcgcgacca tcgcgtcgtt catctcctac gggatcgaga aggcggtgtc gcgccatccc
     1081 gaaaaattcg gccatggcgc gcccgaagga ctagcggcgc cggaaggcgc caacaatgcc
     1141 gataccggcg gcgcgctggt gccgctgctc acgctcggca ttccgggcgg ctcgtcgacc
     1201 gcgatcctgc tctccgcgct catcctgtgg ggcgtgcggc cggggccgct gatgatgcag
     1261 gaatcgcccg acgtgttctg gggcctcgtc gccagcatgt atatcggcaa cgcgatcctg
     1321 ctcctgctga acctgccgct ggtgccgctg ttcgcgcaga tcctgcgggt gcccgtctat
     1381 attctctatc ccggcatcct ggggatttcg gtcgtcggcg cctacagcgt gtcgggccgg
     1441 ctgttcgaca tctggctgct cgccggcttc ggcctgctcg gctggctcat gggcaagctg
     1501 aaatatccgg ccgcgccgct catcctcggc ttcgtgctcg gcagcgccat ggagcgtgcg
     1561 ctgcgccagt cgctcacgat gtcgcagggc gacctcggcc tgttccttac gcggccgctc
     1621 tcggccacca tgctggcgct ggcggcgctg ctgctgctca tgccgctgtt cggacagttc
     1681 aacaggtggc ggctgcgcgc ggtcgaaatg gacgattcgt agggtgggtt agcggcgaag
     1741 ccgcgtaacc caccgtttgt agcccgcatg gagcgaagcg aaatgcgggg acggcggtcg
     1801 acatggaatt cgggaggaaa tgatgacgag agtcgttctg gccgcgttgg cgctgctcgc
     1861 cgcgcttgct ccggccccgg cccaatacaa gccgacgcgg ccggtggagt tcgtcgtgca
     1921 caacgggccg ggctccggca acgacgtgtt cgcgcgcgcg ctcatccagg tgatcgagca
     1981 ggagaagctg atgccggtgc gcatgcaggt cgccaacaag gtgggcggcg gcagcaccac
     2041 ggcggcgtcc tacgtcaccg agaaggcggg cgatcccaac gtgatcgcgg tgttcgtcaa
     2101 catctggctg gtcgatccgc tggtgcagac ggaggcgaaa acgcggctgc tcgacatgac
     2161 gccgatcgcg ctcgtgcaga tcgagccggc gctggtggtg acgcgcgccg attcgccgct
     2221 caagacgctg ggagagttcg tcgcggccgc caaggagaag ccggggcaga tgaagcagtc
     2281 gggcggctcg atcacgtcgc gcgagaacgt ggtgcgccag ctgctgatga acaaatcggg
     2341 cgcgcgctgg tcgttcatct cgtttccggg cggcggcgag cggctcgccg cgctgctcgg
     2401 cggccatgtc gacatgatga tcctcgaccc gagcgaggcg cgcgagcagg tgcgcagcgg
     2461 caagctgcgg gtgctggcgc aggtggcgga aaagcgcctg cccgatttcc aggacgtgcc
     2521 gacgctcaag gaagcgggct tcgacgtgcc cgacgtgccg cagatccgcg gcgtggtcgg
     2581 cccgccaaaa atgccgcccg acgcagtcgc gttctacgcc agcctgttcc agaagacgac
     2641 gcgcacgccg gcctggcagc gttatctcaa ggacaaccag ttccaggatg cgttcctgtc
     2701 gccggaggac acgcgcaagt tcatcgcgca gttcgaggag cgcctgcgcg ggttgctgcc
     2761 gcaggccggg gtcaaggtgg tgcggtgaat tgatcgcgaa tgcattgcga ggaaatcgat
     2821 ggacgatatt gaagaggact ggctgcgcag cgaggtagtc cctaccttcg atcgcatcat
     2881 gcgaggcgaa gaaaaactga tccctgccag tgaggtgttt gccgatatcg agcttcggta
     2941 caacgcccgc aaggcgcaga acagtgtctg aaggcggcca cggcggtggc cgatcatcct
     3001 tcgaggctcg cgctgcgcgc gggcacctca ggatgacggt gataggttgg agtgaacgag
     3061 gatcaacatt atggcccgca tctcgctgat cgaagagcag gaccaccccg agctggcgcc
     3121 gctgatcggg cgcatcaagg ccggccgtcg cggcgggctg ctcaacgtct acaagctgct
     3181 gctgcattcg ccgccgcttg ccgagacctg gctcgatcac gtcggcgcgg tgcgctggaa
     3241 gaccgcgctc accggccggc tgcgcgagtt cgtggtgatc cgcatcgcct acgtcaacgg
     3301 gatcgactac gtgatcaacc agcacgtgcc cgcgctggcg gtcgccgagg gcgtgacgct
     3361 tgccgaatgc gaggcgctca aggactggcg caagtcgagc ctcttcgacg cggccgagcg
     3421 cgccgcgctt gcctatgcgg acgcgatggt gctcgccacc tcggtgccgg acgacgtgtt
     3481 cgagaacctg cgtcgccact acgacgagcg cgccatcgtg gaactctcag tcctcatcgg
     3541 cacgtacctc atgcacaacc gcgtcatgaa ggcgctcgcc atcgacctgg aggcggacag
     3601 cgaacgcggg cggtgaaatt cttcgtagcc cgcatggagc gaagcggaat gcggggacac
     3661 cccatccaac cgataaaccg atcccggatt acgcttcgct ccatccgggc tacgagaaaa
     3721 cccatgcgca gtctcgctct cgtcctcttc gcgctcagcc tcaccgtcac tggtgcatca
     3781 gcccaaaacg acgccaactg gcccaccaag ccgatccgtg tcgtcgttcc gttcccggcc
     3841 ggcagcttcg ccgacatcac gacgcgcatc ctgcagcaga agctggggcc gcggctcggc
     3901 cagcagctcg tgatcgacaa tcgctcgggc gcgagcggca atatcggcgc cgacgtcttg
     3961 gccaagtcgg cgcccgacgg ctacaatttc ggcatcgcga ccaacagcac gcacgcggtg
     4021 gcgaaggcgc tcaatcccaa cctgccctac gacccgatca aggatttcgc cctggtgtcg
     4081 ctgatcggcg acgcgcccta cgtgctcatc acggcgccgg accttgcggc caattcggtg
     4141 caggagctga tcgcgctcgc caagtcgaag ccgggcgcgc tcaactacgc gtcggccgga
     4201 cccgcgagcc tcgcgcatct cgccggcgcg ctgtttgcgc agatgtcggg aacgcagatc
     4261 accgaggtgc cctaccgcag ctcggcacaa tcggtgctcg acacctccga aggccgcatc
     4321 gagatgcagt tcggcaccat ggcgccggtg ctgccgcata tccgggccgg ccgcgtcaag
     4381 gcgctcggcg tcaccagccg caagcgcgcc gaggtgctgc ccgacgtgcc gaccatcgac
     4441 gagtcgggac tgccgggcta cgaggcctcg ctgtggctcg ccgtagtcgc gccggccagc
     4501 acgccgcccg cgatcgtcgc ccgcatggcg cgcgagctc
//
LOCUS       DASPXW010000289         2407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_334129, whole genome shotgun sequence.
ACCESSION   DASPXW010000289 DASPXW010000000
VERSION     DASPXW010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334129"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..2318)
                     /gene="mrcB"
                     /locus_tag="VEL04_06610"
     CDS             complement(18..2318)
                     /gene="mrcB"
                     /locus_tag="VEL04_06610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1B"
                     /protein_id="HYT48074.1"
                     /translation="MSRRSGFKRFLWYTVLTVALLGAGFTLYLDLRVRNEFEGRRFAL
                     PARIYARPLELHAGLRIPLADVEAELRELGYREAVREGESGWFVRTREGLDIALRPFV
                     FWDGPQAARRLRVSFDGPTVTSVRDEEDRDQALVRLEPVPVGGIYPANNEDRVLVRLG
                     EVPKQLVQALIAVEDRNFFSHAGFDPRGLARAAASLVSGGPIQGGSTLTQQLVKNFFL
                     TPERTLQRKFTELIMAMLLELHYDKSEILETYLNEIYLGQDRDRAIHGVGLAALYYFG
                     KPVEQLTLAESALLVGMAKGPAFYDPYRQPRRALERRNLVLRETKDQGFVTMEQYAAA
                     RATTLTLNPKAAVGTSAHPAFLQLVHRQLRREYDEADLRSEGLRIFTTLDPRVQLAAE
                     RVLARKLAQFDREKRFGEPGLEGAIVVTDSQAGEVQALVGGRDARYRGFNRALDAARP
                     VGSLLKPAIYLTALGEPTRYTLVTPIDDGPFVWKSHGAPDWQPSNYDQKFHGMVPLRT
                     ALAQSYNAATARLGTDLGIERVLATVKRLGVERELRPYASTLLGAAEMSPLEVAQMYQ
                     TIASGGFRTPLKAIREVTTQDGRPLRRYPLAIEQAFPPEPMYLLAAAMQDVVRQGTGQ
                     SLKNWLPPETAVAGKTGTTDEQRDAWFAGFTGNRLGIVWIGYDDNRAARLSGAAAALP
                     VWGEMMAALDAEPLALPRPDGIESVLIDPQSGLRADLTCAGAVELPFTMGSAPEQRAP
                     CATEIGVAVEQVKERARGWLERLFRR"
BASE COUNT          342 a          822 c          861 g          382 t
ORIGIN      
        1 cagggcgtag tggtgtttca tcgcctgaac aggcgctcga gccagcctct ggcgcgttcc
       61 ttcacctgct cgacggcgac gcctatctcg gtcgcgcagg gggcgcgctg ctcgggcgcc
      121 gagcccatgg tgaacggcaa ctccacggcg ccggcacagg tcaggtcggc gcgcagcccg
      181 gactgcggat cgatcaatac gctctcgatg ccatccggcc tgggcagggc gagcggctcc
      241 gcatcgagcg cggccatcat ttcgccccag accggcagcg cggcggccgc gccgctcagg
      301 cgcgcggcgc ggttgtcgtc gtagccgatc cagacgatgc ccagccggtt gccggtgaat
      361 ccggcgaacc aggcgtcgcg ctgctcgtcg gtggtgccgg tcttgccggc gacggcggtc
      421 tcgggcggca gccagttctt gaggctctgt cccgtgcctt ggcgcaccac gtcctgcatg
      481 gcggcggcga ggaggtacat cggctcgggc gggaacgcct gctcgatggc gagcggatag
      541 cggcgcagcg gccgtccgtc ctgggtcgtc acctcgcgga tcgccttcag cggcgtgcgg
      601 aacccgccgc tggcgatggt ctgatacatc tgtgccacct ccagcgggct catctcggcc
      661 gcgccgagca gggttgaggc gtacggccgc agctcgcgct cgacgccgag ccgcttcacg
      721 gtggccagga cccgctcgat gccgagatcg gtgcccaggc gcgccgtcgc ggcgttgtag
      781 gactgcgcca gcgccgtgcg cagcggcacc atgccgtgaa atttctggtc gtaattggac
      841 ggctgccagt ccggggcgcc gtggctcttc cagacgaaag gcccgtcgtc gatcggcgtg
      901 acgagggtat agcgcgtggg ctcgcccagc gcggtgaggt agattgccgg cttcagcagc
      961 gatcccactg ggcgggcggc gtcgagcgcg cggttgaagc cgcgatagcg cgcgtcgcgc
     1021 ccgccgacca gcgcttgcac ctcgccggcc tggctgtcgg tgaccacgat cgcgccttcg
     1081 agacccggct cgccgaagcg cttctccctg tcgaactgcg ccagcttgcg ggcgaggacg
     1141 cgctcggcgg cgagctgcac gcgcggatcc agcgtggtga agatgcgcag cccttccgag
     1201 cgcaggtcgg cctcgtcgta ttcgcgccgc agctgccggt gcacgagctg gaggaaggca
     1261 gggtgcgccg aggtgccgac cgccgccttc gggttcagcg tgagggtcgt ggcccgcgcg
     1321 gcggcgtact gctccatggt gacgaagccc tggtccttgg tctcgcgcag caccaggttg
     1381 cggcgctcga gcgcgcgcct gggctggcga taggggtcgt agaacgccgg gcccttcgcc
     1441 atgccgacga gcagcgcgct ttcagcgagg gtgagctgct cgacgggctt gccgaagtaa
     1501 tacagcgcgg cgagcccgac gccgtggatc gcgcggtcgc ggtcctggcc gagatagatc
     1561 tcgttcaggt aggtctcgag gatctcgctc ttgtcgtaat gcagctcgag cagcatcgcc
     1621 atgatcagct cggtgaattt gcgctgcagg gtgcgttccg gcgtgaggaa gaagttcttc
     1681 accagctgct gcgtcagcgt gctgccgccc tggatggggc cgccggagac caggctcgcc
     1741 gcggcgcgcg ccagcccgcg cgggtcgaag cccgcgtgcg agaagaagtt gcggtcctcg
     1801 accgcgatca gcgcctgcac cagctgcttc ggcacctcgc cgagcctgac cagcacccgg
     1861 tcctcgttgt tcgcggggta gatgccgccg accggcaccg gctcgaggcg caccagcgcc
     1921 tggtctctgt cctcctcgtc gcgcaccgag gtcaccgtgg ggccgtcgaa cgacacgcgc
     1981 aggcgtcttg ccgcttgcgg cccgtcccag aacacgaacg gccgcagcgc gatgtcgagc
     2041 ccctcgcgcg tgcgcacgaa ccagccggac tcgccttcgc gcaccgcctc gcggtagccg
     2101 agctcgcgca gctccgcctc gacgtccgcc agcgggatgc gcagtccggc gtgcagctcg
     2161 agcggccgcg cgtagatgcg cgccggcagg gcgaagcggc ggccctcgaa ctcgttgcgc
     2221 acgcgaaggt cgaggtacag ggtgaacccg gcccccagca gcgcgaccgt gagcaccgta
     2281 taccagagga atcgtttgaa accggagcga cgcgacatca gccttccatt ttacttgttg
     2341 cagcggcctc gaattgcagc cgggataggt ggattagact gcgggcgaaa tagttcacag
     2401 gtcacgc
//
LOCUS       DASPXW010000290         4449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_587785, whole genome shotgun sequence.
ACCESSION   DASPXW010000290 DASPXW010000000
VERSION     DASPXW010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587785"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..936
                     /locus_tag="VEL04_06615"
     CDS             100..936
                     /locus_tag="VEL04_06615"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYT48075.1"
                     /translation="MKWFAAALVALCAACASKPAVNTSQAQSRLAEAEARAAVLVRSG
                     DLGGAARQYDEAVRIAASVENVDAVAANSVNLSIVYQWLGRDAEAREVLARMLDDPRR
                     AFSERRRLQAELRRAIVELALQNTDAAATWVERAARRCPDFACEYAATILNLQAQVAL
                     AASRPADAARLASSAAERARARGDRSEAANALRALGRARRAQGEPSAAIPVLEQALAL
                     DRELADPRKILADLTELSLASSAAGDRQAARDYYERALAVSRAVQDVHGIAEMEAQLR
                     RP"
     gene            complement(956..1552)
                     /locus_tag="VEL04_06620"
     CDS             complement(956..1552)
                     /locus_tag="VEL04_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYT48076.1"
                     /translation="MATKSNAERPSARKRIFDTASELFYRRGIRAVGVDAIAAQADTT
                     KMSLYRSFPSKDDLVAEWLRGHDVKFWQTWDSMSQKYLGDARKQLQAAFALLAKHVSD
                     PSARGCPMANAAVEITEKDHPARKVIETHKAKLRGRLAALCEQAGAREPALLADALFL
                     LMEGAQVSVILGVRGPVQNVARAADALIESHLHVAPQA"
     gene            1600..1923
                     /locus_tag="VEL04_06625"
     CDS             1600..1923
                     /locus_tag="VEL04_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48077.1"
                     /translation="MHPATTSRHSHPSKIARAAACALTLGLVSAASLAQSLFDETRFK
                     KQFPPTLTPDTASAEALARRAPGSGVDKKVTPDAERGSGSEVAKPSADSELRQAPDRK
                     AFPTK"
     gene            1920..2489
                     /locus_tag="VEL04_06630"
     CDS             1920..2489
                     /locus_tag="VEL04_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48078.1"
                     /translation="MKEKAMWMRASLIAMSTAAAAMTVGCSPQPSAVAAPINDKVYSV
                     VPASVKVKAGIVSGEVTEMKVMERVEEGSGRIASPAKLSGKLVLKNTSRDQSVRLIAG
                     KISYIDSQGRAIKLEENRTEPTIRVAASYGSSDRLDPGQEVTQVVDAEFPAEALKLKK
                     LKDIRIELSYIPAPFKEETFNFPVSIISK"
     gene            2486..2806
                     /locus_tag="VEL04_06635"
     CDS             2486..2806
                     /locus_tag="VEL04_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48079.1"
                     /translation="MTDELIDLVASIEMHRQEIEREAGLEASRKYAMGRLQEALHAEQ
                     APENREALAAALKRVQALGSRASERPTPRPGRPAPQGRSWREAQGRSPRTRGRRTMGR
                     GGGR"
     gene            complement(2823..4172)
                     /gene="ffh"
                     /locus_tag="VEL04_06640"
     CDS             complement(2823..4172)
                     /gene="ffh"
                     /locus_tag="VEL04_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HYT48080.1"
                     /translation="MLDNLTARLAKIVKQIRGEARLTEANIQDALREVRVALLEADVA
                     LPVVKHFIDAVRSKALGEEVVGSLTPGQALVGVVHRELAALMGEANVGLNLATQPPAV
                     ILMAGLQGSGKTTTCGKLARLLLADGKKVLLVSCDVYRPAAIEQLRTVASQAAVDFFE
                     SKAGENPVDIAHRALEHARTRYYDVLIVDTAGRLAIDEEMMREIAALHAALNPVETLF
                     IVDAMQGQDAVNVARAFSERLPLTGVILTKLDGDARGGAALSVRHVTGKPIKFAGVGE
                     KLAGIEPFHPERMAARVLGMGDIVSLVEEAHAKIDLEQAQKLAEKVKKHGHFDLEDFK
                     QQITQMTKMGGVASLMDKLPAQLVGQIDPAKLQDEKQFKRIEGIINSMTPQERRKPEL
                     IKASRKRRIAAGAGVPVQVVNQLLNQFEQMQKMMKMMKGGNLQRMMRNMKGMLPGMR"
     gene            4192..>4449
                     /locus_tag="VEL04_06645"
     CDS             4192..>4449
                     /locus_tag="VEL04_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48081.1"
                     /translation="MPDIVIHVIASAAYVGLAWHFWRTRWRHQATPGSAPRMAERVAI
                     LAVLGIHGTLLYAEIFAAPQLRFGFAQALAVMIWLAVLICWV"
BASE COUNT          747 a         1472 c         1482 g          748 t
ORIGIN      
        1 cgccaagcgc atgtggccgc tcagcgattc gttcaaggaa gtcaccgacg gcgagctgcc
       61 gatcgacagc ttcgacgcgc gcggcaaggc acctcgccta tgaaatggtt cgccgctgcg
      121 cttgtcgcct tgtgcgcggc atgcgcatcc aagccggccg tcaacacttc gcaggcgcaa
      181 agccgactcg ccgaagccga agcgcgcgcc gcagtgctgg tgcggtctgg cgacctgggc
      241 ggcgccgcgc gccagtacga cgaggcggtg cgcatcgccg cctcggtcga gaacgtggac
      301 gcggtcgcgg cgaactcggt gaacctgtcg atcgtgtacc agtggctcgg gcgcgacgcc
      361 gaagcgcgcg aggtgcttgc gcgcatgctc gacgatccgc gccgcgcgtt ttcggagcgc
      421 cgcaggctgc aggcggaact gcgccgtgcg atcgtagagc tcgccttgca gaacaccgac
      481 gccgcggcca cctgggtcga gcgcgcggca aggcgctgcc cggatttcgc ctgcgagtac
      541 gccgcgacga tcctgaacct ccaagcccag gtggcgctcg cagcgtcgcg ccccgccgac
      601 gcggcgcgcc tcgcgagtag cgcggccgag cgcgcccgcg cccgcggcga ccgctcggaa
      661 gcagccaacg cgctgcgagc cctcggccgc gcgcgtcgcg cgcaaggcga gccctcggcc
      721 gccatcccgg tcctcgagca ggcgctcgcg ctcgaccgcg agcttgccga tccgagaaag
      781 atcctcgccg acctcaccga gctttcgctc gccagctcag ccgcgggcga ccgccaggcg
      841 gcgcgcgact attacgagcg cgcgctcgcg gtcagccgcg cagtgcagga cgtgcacggc
      901 atcgcggaga tggaggcaca actgagacgg ccgtgacggc cggtaagcgt ctgggctagg
      961 cctgaggcgc gacgtgcagg tgggactcga tcagcgcatc ggcggcgcga gcgacgttct
     1021 gcaccgggcc gcggactccc agaatgacgc tgacctgagc accctccatc agcagaaaga
     1081 gagcgtcggc cagcagcgct ggctcccgcg ctcccgcctg ctcgcacaat gccgccagcc
     1141 gcccgcgcag cttggccttg tgagtctcga tcaccttcct cgccggatgg tccttctcgg
     1201 tgatctcgac cgccgcgttc gccatcgggc agccgcgggc cgagggatct gaaacgtgct
     1261 tggcgagcag cgcgaaggcc gcctgcagct gcttgcgggc gtcgccgaga tatttctgcg
     1321 acatcgaatc ccacgtttgc cagaacttca cgtcgtgccc gcgcagccat tcggccacca
     1381 ggtcgtcctt cgacggaaag ctgcggtaca ggctcatctt ggtggtgtcg gcctgggcgg
     1441 cgatggcatc gacgcccacc gcgcggatgc ccctgcgata aaacagctcg gaagccgtgt
     1501 cgaaaatgcg cttgcgggcg ctcgggcgtt cagcattgct tttcgtggcc atcagaacat
     1561 tccgcttgac tggtgagtga ccggtcagta tcgtagcacg tgcatcctgc aacaaccagc
     1621 cggcattcgc atccgtcgaa aattgcccgt gccgccgcct gcgcgctgac gctgggactg
     1681 gtgtccgcag cgtctctcgc gcagtcgctg ttcgacgaga cccgattcaa gaaacagttc
     1741 ccgccgacct tgacgcccga cactgcatcc gcggaagcgt tggcgcggcg cgcgcccggg
     1801 tccggggtcg ataaaaaagt gacgcccgac gccgagcggg gctccggcag cgaggtcgcc
     1861 aagccctccg cagattcgga attgcggcag gcaccggacc gcaaggcatt tccaaccaag
     1921 tgaaggagaa ggcaatgtgg atgcgagcaa gtttgattgc tatgagcacg gcagcggcgg
     1981 cgatgaccgt cggctgttcc ccccagccgt cggcggtggc tgcgccaatc aatgacaagg
     2041 tctactcggt cgtgcccgct tcggtgaagg tgaaagccgg catcgtgagc ggcgaggtca
     2101 ccgaaatgaa agtgatggag cgcgtcgaag aaggctcggg ccgcatcgcg tcgcccgcga
     2161 agctgagcgg caagctggtg ttgaaaaaca cgtcacgcga ccagagcgtg cgtctgatcg
     2221 caggcaagat ctcttacatc gactcgcagg ggcgcgccat caagctcgag gagaatcgca
     2281 ccgagccgac gatccgcgtc gccgcgtcct acggttcctc cgaccggctc gatcctgggc
     2341 aggaagtgac ccaggtcgtc gatgccgagt tcccggccga ggcgttgaag ctgaagaagc
     2401 tgaaggacat ccgcatcgag ctttcctata tccccgcgcc gttcaaggaa gagaccttta
     2461 acttcccggt gtcaatcatc agcaagtgac agacgagctg attgatcttg tcgccagcat
     2521 cgaaatgcac cggcaggaga tcgaacgcga ggcgggcctg gaggcttcgc gcaaatacgc
     2581 gatgggacgg ctgcaggaag ccctgcatgc cgagcaggcc ccggaaaacc gggaggcgct
     2641 cgccgccgcc ctaaagcggg ttcaggcgct gggcagtcgc gcgagcgaga ggccgacgcc
     2701 caggccgggc cggcccgctc cacaaggaag atcgtggcgc gaggcgcaag gccgctcacc
     2761 gaggacacgg ggcagacgga ccatgggccg cggcggcgga cgctagtccg ccgcgagccc
     2821 ggctagcgca ttccgggcag catgcccttc atgttgcgca tcatgcgctg caggttgccg
     2881 cccttcatca tcttcatcat tttctgcatc tgctcgaact ggttgagcag ctggttgacg
     2941 acctggacgg gaacgccggc cccggcggct atgcggcgct tgcgcgaagc cttgatcagc
     3001 tcgggctttc tgcgctcctg cggggtcatc gagttgatga tgccctcgat gcgcttgaac
     3061 tgcttctcgt cctgaagctt ggcaggatcg atctggccga cgagctgggc gggtagcttg
     3121 tccatgagag aggcgacgcc gcccatcttt gtcatttgcg tgatctgctg cttgaagtcc
     3181 tcgaggtcga agtggccgtg ctttttaact ttttctgcaa gtttttgagc ctgttcgaga
     3241 tcgatcttgg cgtgcgcctc ctcgaccagc gagacgatgt cgcccatgcc gagaacgcgc
     3301 gccgccatgc gctcgggatg gaacggctcg atccccgcga gcttctcgcc gacgccggcg
     3361 aacttgatcg gcttgccggt gacgtggcgc acggagaggg ccgcgccacc gcgggcatct
     3421 ccatccagct tggtgaggat cacgccggtc agcggcagcc gctcgctgaa tgcgcgcgcc
     3481 acgttgaccg cgtcctgacc ctgcatggcg tcgacgatga acagcgtctc gaccggattc
     3541 agcgcggcat gcagcgctgc gatctcgcgc atcatctcct cgtcgatcgc gagacgtccc
     3601 gcggtgtcga cgatgaggac gtcgtaatag cgggtgcggg cgtgctcgag cgcgcggtgc
     3661 gcgatgtcga cggggttttc accagctttt gattcaaaaa aatcaaccgc tgcctggctg
     3721 gcgacagtcc gcagctgctc gatcgcagca ggccgataaa cgtcgcacga cacgagcagc
     3781 actttcttcc cgtcggcgag aagcagtctc gcgagcttgc cgcaagtggt ggtcttgcca
     3841 gagccctgca gccccgccat caggattacg gcgggcggct gggtcgcgag attcaagccg
     3901 acgttcgcct cgcccatcag ggctgcgagc tcgcggtgca ccacgccgac cagcgcctgg
     3961 ccgggggtga ggctgccgac cacttcctcg cccagggcct tcgagcgcac cgcgtcgatg
     4021 aagtgcttca ccaccggcag cgcgacgtcg gcctcgagca gcgccacgcg cacttcgcgc
     4081 agcgcgtcct ggatgttcgc ttcggtgagg cgcgcttcgc cgcgaatctg cttcacgatc
     4141 ttggcgaggc gcgcggtcag gttgtccagc atggggtgat aaactgaaga ggtgcccgac
     4201 attgtaattc acgtcatcgc gtccgccgcg tacgtgggac tggcgtggca cttctggagg
     4261 acgcgctggc gccatcaggc gacgccgggc agcgctccgc gcatggcgga acgcgtcgcc
     4321 atcctcgctg tcctgggcat ccacggcacg ctgctctatg cggagatttt cgcggcgccg
     4381 cagctgcgct tcggcttcgc gcaggcgctc gccgtgatga tctggctggc cgtgctcatc
     4441 tgctgggtt
//
LOCUS       DASPXW010000291         3746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_208462, whole genome shotgun sequence.
ACCESSION   DASPXW010000291 DASPXW010000000
VERSION     DASPXW010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208462"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VEL04_06650"
     CDS             <1..370
                     /locus_tag="VEL04_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009455980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HYT48082.1"
                     /translation="LVPDSGGTFYLPRLVGSARAMGLALLGEKLSAEDAERWGLIWKV
                     VDDENLLPAATTLARTLAGGPTKGYGLLKKALYASPGNSLDAQLDLERDLQREAGFSE
                     DYREGVTAFKEKRQPKFKGR"
     gene            367..1626
                     /gene="paaK"
                     /locus_tag="VEL04_06655"
     CDS             367..1626
                     /gene="paaK"
                     /locus_tag="VEL04_06655"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HYT48083.1"
                     /translation="MKKFPLEAIERASQKKLKEVQLKRLQWSLKHAYDRVPHYRKKFD
                     AAGVKPDACRALEDLARFPFTTKADLRETYPFGMFAVPMERIVRIHASSGTTGKPTVV
                     GYTAKDIDTWTQVMARSIRAAGARPGDKVHVAYGYGLFTGGLGAHYGAERLGLAVIPL
                     GGGMTERQVQLINDFKPEIIMVTPSYMLAIADEMARQGLDAKQSSLRIGIFGAEPWSN
                     EMRATIEKRLSLDAMDLYGLSEVIGPGVAQECIETKDGLTVWEDHFYPEVVDPASGEP
                     LPEGEKGELVFTSLTKEALPVIRYRTRDLTRLLPGGARTMRRMEKITGRSDDMLIIRG
                     VNVFPSQIEELILKQPGLSPHYVLELSRDGPLDHLTVLVEGDDASRAGLRVSIKSYIG
                     ITADVKLGSIERSIGKAKRVIDKRPKG"
     gene            1640..2377
                     /locus_tag="VEL04_06660"
     CDS             1640..2377
                     /locus_tag="VEL04_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYT48084.1"
                     /translation="MKGLAGKSVLVTGGGGAIGRVICKRFAEEGASLLVADRNEKNAL
                     EVASQVQGRAIVFDISDYAAAKQALGSETIDILVNNAGWDRFQNFADTKPEEWATLIA
                     INLRGPLNMHHLVLPQMLERGRGRIVNISSDAARVGSSGEAVYSACKGGMISFSKTLA
                     REVARKGITVNVVCPGPTDTPILRGFLGEGEAGQKVYDALVRAIPMKRVGQPGDIPGI
                     VAFLASDDAGFITGQVISVSGGLTMAG"
     gene            2428..3237
                     /locus_tag="VEL04_06665"
     CDS             2428..3237
                     /locus_tag="VEL04_06665"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HYT48085.1"
                     /translation="MQNALDKARGYLDARRGERVTLDELARAVGASPFHLQRSFKQAF
                     GVSPRGYQDAHRVESVKSSLRNGSRVTDALFEAGYGSVSRFYEKPHLGMKARDYRAGG
                     KGQTIRFGCFVTPLGTVLAAATEKGLCSIKLGDDAAKLRRLLADEFSQADLVEDALDE
                     LKGKILAFIAGEASLARIPLDIRGTVFQRRVWDVLRRIPRGETRTYGDIARAIGKPRA
                     VRAVGSACGANPVALVVPCHRAVRSDGGLGGYAWGISRKERLLALEKRGRK"
     gene            complement(3240..>3746)
                     /locus_tag="VEL04_06670"
     CDS             complement(3240..>3746)
                     /locus_tag="VEL04_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006761964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HYT48086.1"
                     /translation="LPVLLLALGFAAPSAGETPGLGQPVSESEIRAWDISILPDGSGL
                     PPGSGTPAQGARIYAQKCVACHGENGKGGPFAPVVGGGPLTSINSVKTIANFWAQATT
                     VFDFIRRAMPWQQPRTLTNDEVYALTAYILAQNKLIGDNAVMNAETLPKVRMPNRDGF
                     IVRFPDRM"
BASE COUNT          675 a         1215 c         1271 g          585 t
ORIGIN      
        1 cctggtgccc gattccggcg gcacttttta cctgccgcgg ctggtcggca gcgcgcgcgc
       61 catggggctc gcgctgctcg gcgagaagct ctcggccgag gacgccgagc gctggggact
      121 gatctggaag gtggtcgatg atgagaacct gcttcccgca gcgaccacgc tggcgcggac
      181 gcttgcgggc ggcccgacga aaggctatgg gctgctcaag aaagcgcttt atgcctcgcc
      241 gggcaactcg ctcgacgcgc agctcgacct cgagcgcgac ctgcagcgcg aggcgggctt
      301 ttcggaggac taccgcgagg gcgtgaccgc tttcaaggaa aaacggcaac cgaagttcaa
      361 aggccgatga agaaatttcc ccttgaggcg atcgagcgcg cctcccagaa aaaactgaaa
      421 gaagtccaat tgaaaaggct tcagtggtcg ctcaagcatg cttacgaccg cgtgcctcac
      481 tacaggaaga aattcgacgc cgcgggcgtg aagccggacg cttgccgcgc gctcgaagac
      541 ctggcgcgct ttcccttcac caccaaggcg gatctgcgcg agacctatcc gttcggcatg
      601 ttcgcggtgc cgatggagcg catcgtgcgc atccacgcct cctcggggac caccggcaag
      661 cccaccgtgg tcggctatac ggcgaaggac atcgatacct ggacgcaggt catggcgcgc
      721 tcgatccgcg ccgccggcgc caggccaggc gacaaggtgc acgtcgccta cggctacggc
      781 ctgttcaccg gcgggctggg cgcgcattac ggcgcggagc gcctcggcct cgcagtgatc
      841 ccgctcggcg gcggcatgac cgagcgccag gtgcagctga tcaacgactt caagcccgag
      901 atcatcatgg tgacgccgag ctacatgctg gcgatcgccg acgagatggc acggcagggg
      961 ctcgatgcga agcaatcgtc gctgcgcatc ggcatcttcg gcgccgagcc ctggagcaac
     1021 gagatgcggg cgacgattga aaaaaggctg tccctcgatg ccatggacct ctatggcctg
     1081 tcggaagtga tcggccccgg cgtggcccag gaatgcatcg agaccaagga cggcctcacg
     1141 gtctgggagg accatttcta tcccgaggtc gtcgacccgg cgagcggcga gccgctgccg
     1201 gaaggcgaaa agggcgagct ggtgttcacc tcgctcacca aggaggcgct gccggtgatc
     1261 cgctaccgca cccgcgacct cacccggctc ctgccgggcg gcgcgcgcac catgcgccgc
     1321 atggaaaaga tcaccgggcg ctccgacgac atgctgatca tccgcggcgt caacgtgttc
     1381 ccttcccaga tcgaggaact gatcctcaag cagccgggcc tcagcccgca ttacgtgctg
     1441 gaactgtcca gggacgggcc gctggatcat ttgacggtgc tggtggaagg cgacgacgct
     1501 tcgcgggcgg gccttcgagt cagcatcaag tcctacatcg gcatcaccgc cgacgtgaag
     1561 ctcggcagca tcgagcgctc gatcggcaag gcgaagcgcg tcatcgacaa gcgaccaaag
     1621 ggctaagagg cagaacgaaa tgaaggggct cgccgggaaa tcggtgctgg tcacgggcgg
     1681 cggcggcgcc atcggccgcg tcatctgcaa gcgttttgcc gaggaaggcg cgagcctgtt
     1741 ggtggccgac cgtaacgaaa aaaatgcctt ggaagtcgct tctcaggtcc aaggtcgagc
     1801 aatcgtcttc gacatctccg actacgcggc agccaagcaa gccctgggca gcgaaaccat
     1861 cgacatcctc gtcaacaacg ccggctggga ccgcttccag aacttcgccg acaccaagcc
     1921 cgaggaatgg gcaacgctga tcgccatcaa cctgcgcggc ccgctcaaca tgcatcacct
     1981 ggtgctgccg cagatgctcg agcgcggccg cgggcgaatc gtcaatatct cgtccgacgc
     2041 ggcgcgcgtc ggctcttcgg gcgaggcggt ctactcggcc tgcaagggcg gcatgatctc
     2101 gttctcgaag acgctcgcgc gcgaggtggc gcgcaagggc atcacggtca acgtggtgtg
     2161 ccccggcccc accgacacgc cgatcctgcg cggtttcctc ggcgagggcg aagcgggcca
     2221 gaaggtctac gacgccctgg tgcgcgcgat cccgatgaag cgcgtcggcc agcccggcga
     2281 cattcccggc atcgtggcct tcctcgcctc cgacgacgcc ggattcatca ccggccaggt
     2341 gatcagcgtc tcaggcggcc tgaccatggc cggctaagcg caagaaccgg atagaagaaa
     2401 ctcggcataa gagtcagaat tcgacccatg caaaacgctt tagacaaggc acggggctac
     2461 ctcgatgcgc gtcgcggcga gcgcgtgacg ctcgatgagc tggcgcgcgc cgtgggcgca
     2521 agcccgtttc acctgcagcg cagcttcaag caggcgttcg gagtgtcgcc gcgcggctac
     2581 caggacgcgc accgcgtcga gtcggtgaaa tcctcgctgc ggaacggcag ccgcgtcacc
     2641 gacgcgctgt tcgaggcggg ctacggctcg gtgtcgcgct tctacgagaa gccgcatctc
     2701 ggcatgaagg cgcgcgacta tcgcgccggg ggcaagggcc agacgattcg ctttggctgc
     2761 ttcgttacgc cgctagggac ggtgctggcc gcggcgaccg aaaagggact gtgctcgatc
     2821 aagctcggcg atgacgccgc gaagctgcgg cgcctgctcg ccgacgagtt cagccaggcc
     2881 gatctggtgg aggatgcgct ggacgaattg aaagggaaga tccttgcctt catcgcaggc
     2941 gaggcgagcc tcgcgcgcat tccgctcgat atccgcggca cggtgttcca gcgccgcgtg
     3001 tgggacgtgc tgcgccgcat cccgcgcggc gaaacccgca cctacgggga cattgcgcgc
     3061 gccatcggca agccgcgcgc ggtgcgcgca gtcggcagcg cctgcggcgc gaatcccgtg
     3121 gcgctggtgg tgccctgcca ccgcgcagtg cgctcggacg gcggcctcgg cggctacgcc
     3181 tggggaatct cgcgcaagga gcgcctgctc gcgctcgaaa agcgcggccg caagtagcgc
     3241 tacatccggt ccgggaagcg cacgatgaag ccgtcgcggt tcggcatgcg cactttcggc
     3301 agcgtctcgg cattcatgac tgcgttatcg ccgatcagct tgttctgcgc caggatgtag
     3361 gcggtgagcg catagacctc gtcgttggtc agcgtgcgcg gctgctgcca cggcatcgcg
     3421 cgccggatga agtcgaatac ggtcgtggcc tgcgcccaga agttggcgat ggtcttgacc
     3481 gagttgatgc tggtgagcgg cccgccgccg actaccggag cgaacgggcc gcctttgccg
     3541 ttctcgccgt ggcaagcgac gcacttctgc gcgtagatgc gcgcgccttg cgcaggggtg
     3601 ccgctgcccg gcggcaggcc gctgccgtcg ggcaggatgc tgatgtccca ggcgcgaatc
     3661 tcggattcgg aaactggctg tcccaggccg ggagtctcgc ctgcgctcgg ggcagcgaag
     3721 ccgagggcga ggagcaggac cggcaa
//
LOCUS       DASPXW010000292         2789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1470329, whole genome shotgun sequence.
ACCESSION   DASPXW010000292 DASPXW010000000
VERSION     DASPXW010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1470329"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /gene="atpG"
                     /locus_tag="VEL04_06675"
     CDS             <1..770
                     /gene="atpG"
                     /locus_tag="VEL04_06675"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HYT48087.1"
                     /translation="RKAQDRMRHARPYGEKIRNVAAHISHANPEYRHPFLVHRDSVKR
                     VGVIVITTDKGLCGALNTNLLRMVLNQYKAWQAEGEEVDVCCIGNKGFGFMQRLGANI
                     ASHAIQIGDRPQMDKLIGTIKVMLDGYTKDRFDRLVIGYTRFVNTMKQEPVLEQLLPL
                     SGERLGAPETVWDYLYEPEARAVLDQVMTRYIEAIIFQAVAENMASEQSARMVAMKAA
                     SDNARDLIDELTLIYNKNRQAGITKELSEIVGGAAAV"
     gene            797..2227
                     /gene="atpD"
                     /locus_tag="VEL04_06680"
     CDS             797..2227
                     /gene="atpD"
                     /locus_tag="VEL04_06680"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HYT48088.1"
                     /translation="MAQTQGTIVQCIGAVTDIEFPREGMPGIYDALKLVDTAEGGLAQ
                     KGLTFEVEQQLGDGVVRCIALGSSDGLRRGMKVVSTGAPISVPVGLGTLGRIMDVLGR
                     PIDDGGPIKSDKLRSIHQAAPKFDELSPSVELLETGIKVIDLICPFAKGGKIGLFGGA
                     GVGKTVNMLELINNIATQHSGLSVFAGVGERTREGNDFYHEMIEAEVVKLDNLPESKV
                     AMVFGQMNEPPGNRLRVALTGLTMAESFRDEGRDILFFVDNIYRFTLAGTEVSALLGR
                     MPSAVGYQPTLAEEMGRLQERITSTKTGSITSVQAIYVPADDLTDPSPATTFGHLDAT
                     VVLSRDIASLGIYPAVDPLDSTSRQVDPNTIGEDHYNTTRAVQATLQRYKELRDIIAI
                     LGMDELAPEDKQAVARARKIQRFLSQPFTVAQNFTGTPGKYVSLKDTIKGFKMIVNGE
                     CDALPEQAFYMVGPIEEAIEKAKTLQ"
     gene            2227..2646
                     /locus_tag="VEL04_06685"
     CDS             2227..2646
                     /locus_tag="VEL04_06685"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HYT48089.1"
                     /translation="MANTIHVDVVSAEESIYSGEAEFVVLPGEAGELGIYPRHAPLIT
                     RIKPGAVRIKPAGGGEEQLIFVAGGILEVQPKLITVLADTAIRGADLDEAKANDALQR
                     AEQARRSAKDKQEIAVVEGELAMLAAQLAAIRKLRKK"
BASE COUNT          568 a          870 c          913 g          438 t
ORIGIN      
        1 tgaggaaggc gcaggaccgc atgcgccacg cgcggcccta tggcgagaag atccgcaacg
       61 tcgccgcgca catcagccat gccaatccgg agtaccggca cccgttcctg gtgcaccgcg
      121 actccgtcaa gcgcgttggc gtgatcgtga ttaccaccga caagggtctg tgcggggcgc
      181 tgaacaccaa cctgctgcgc atggtgctga accagtacaa ggcatggcag gcagaaggcg
      241 aggaggtcga cgtctgctgc atcggcaaca agggcttcgg cttcatgcaa cgcctgggcg
      301 cgaatatcgc gtcgcacgcg atccagatcg gcgaccggcc gcagatggac aagctgatcg
      361 gcaccatcaa ggtgatgctc gacggctata ccaaggaccg cttcgaccgc ctggtgatcg
      421 gctatacccg ctttgtgaac accatgaagc aggagccggt gctggagcaa ttgctgccgc
      481 tttcgggcga gcggctgggc gcgccggaga cagtgtggga ctacctctat gaaccggagg
      541 cgagggcggt gctcgaccaa gtgatgacgc gctacatcga ggcgatcatt ttccaggcgg
      601 tggccgaaaa catggcttcc gagcaatcgg cgcgcatggt ggcgatgaag gccgcctcgg
      661 acaacgcgcg cgacctgatc gacgagctga cgctgatcta taacaagaac cgccaagccg
      721 gcatcacgaa ggaactgtct gaaatcgtgg gcggcgcagc cgccgtatag cgggacaaag
      781 gaaccaggaa ctcaccatgg cacaaaccca aggcaccatc gtccaatgca tcggcgcggt
      841 caccgatatc gagtttccgc gcgaaggcat gcccggcatt tacgacgcgc ttaagcttgt
      901 tgatactgcc gagggcgggc tggctcaaaa aggcctcacc ttcgaagtcg agcagcaact
      961 cggcgacggt gtggtgcgtt gcatcgcgct ggggtcctcg gacgggctgc gccgcggcat
     1021 gaaggtggtc agcaccggtg cgccgatctc ggttccggtg ggcctgggca cgctcggccg
     1081 catcatggac gtgctcggcc ggccgatcga cgacggcggc ccgatcaaat ccgacaagct
     1141 gcgctcgatc caccaggcgg cgccgaagtt cgacgagctt tcgccttcgg tggagctgct
     1201 cgaaaccggc atcaaggtga tcgacctcat ctgcccgttc gccaagggcg gaaagatcgg
     1261 cctgttcggc ggcgcgggcg tcggcaagac cgtgaacatg ctcgagctaa tcaacaacat
     1321 cgccacccag cacagcggac tctcggtgtt cgcgggcgtg ggcgagcgta ctcgtgaagg
     1381 caacgacttc taccacgaga tgatcgaagc cgaggtggtg aagctcgaca acctgcctga
     1441 atcgaaagtg gcgatggtgt ttggccagat gaacgagccc ccgggcaacc gcctgcgcgt
     1501 ggcgctcacc ggcctcacca tggcggagag cttccgcgac gagggccgcg acatcctgtt
     1561 cttcgtcgac aacatctacc gtttcacgct tgcgggaacc gaagtgtcgg cgctgctcgg
     1621 ccgcatgcct tcggccgtgg gctaccagcc gacgctggcg gaagaaatgg gccgcctgca
     1681 ggagcgcatc acttcgacca agacaggttc gatcacctcg gtgcaggcga tttacgtgcc
     1741 tgcggacgac ctgaccgacc cgtcgcccgc gaccaccttc ggtcacctcg acgcgaccgt
     1801 ggtactttcg cgcgacatcg cgtcgctcgg catctacccg gcggtggatc cgctcgactc
     1861 gacctcgcgc caggtcgatc cgaacaccat tggcgaggac cactacaaca cgacgcgcgc
     1921 ggtgcaggcg acgctgcagc gctacaagga gctgcgcgat atcatcgcca ttctcggcat
     1981 ggatgagcta gcgcccgagg acaagcaggc ggtggcccgc gcgcgcaaga tccagcgctt
     2041 cctgtcgcag ccgttcaccg tggcgcagaa cttcaccgga acgccgggca agtacgtctc
     2101 gctcaaggac accatcaagg gcttcaagat gatcgtcaac ggcgaatgcg acgcgctgcc
     2161 ggagcaggcc ttctacatgg tcggcccgat cgaagaggcc atcgagaagg cgaagacgct
     2221 gcagtaatgg caaatacgat tcacgttgac gtggtcagcg ccgaggagtc tatctactcg
     2281 ggcgaggccg agttcgtcgt cctgccgggc gaggcgggcg agctcggcat ctacccgcga
     2341 cacgcgccgc tcatcacgcg gatcaagccc ggcgccgtgc gcatcaagcc ggccggcgga
     2401 ggcgaggagc agctgatctt cgttgcgggc ggcatactcg aggtgcagcc gaagctgatc
     2461 acggtgcttg cggatactgc tatccgtggc gccgacctcg acgaggcgaa agcgaacgac
     2521 gcgctccagc gcgccgagca ggcgcgccgc agcgccaagg acaagcagga aatcgcggtc
     2581 gtggaaggcg aactggcgat gcttgcggcg cagctcgccg cgatcaggaa actgcgcaag
     2641 aagtagcgcc ccggcgtcgc gattgccacc tcgtcgagag cgaagccgga tgaagcggtg
     2701 atcgactagg gaagctctgc gtaagtgact gaaacgatgc ggccgatgtc gatcatgcgc
     2761 ggtcctgatg tcgaacgacg cctgaaagc
//
LOCUS       DASPXW010000293         2262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_209054, whole genome shotgun sequence.
ACCESSION   DASPXW010000293 DASPXW010000000
VERSION     DASPXW010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209054"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="VEL04_06690"
     CDS             <1..839
                     /locus_tag="VEL04_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48090.1"
                     /translation="MVMQVLLIAVQNSVDYEHLGVATSGTMLFRSLGGALGVALFGAI
                     FANRLHAQLAGPGMDFLATVIPAAVKGLPPDLHQEYIAAVMAALRPVFLAAAGISALG
                     FVLTFLLREYPLREGAPAEGVGESFAMPRDATSLEELERIITLLVARENRWRLYADLA
                     RRAAIELPAPELWMLARLGERAPITLTSLSAELKIPQPQLASPLHALCERGIAEKNAN
                     GELELTSTGVAMRDRIVTARRKGLADMLARWRPEEHPDVLALIERMAQALTSDLPAPK
                     AA"
     gene            complement(896..1780)
                     /locus_tag="VEL04_06695"
     CDS             complement(896..1780)
                     /locus_tag="VEL04_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48091.1"
                     /translation="MKIVQTDEIPLKRGLEHRGGTFHGRIMVEGEPGALDNFQLSFGQ
                     MGGDFNSPRHRHNFEQIRYQLEGVLDYGRDGKLVAGMVGYFPEAVYYGPQSQDPDIAC
                     KTIVLQFGGASGSGYLSQAEVQAGTRELRAEGEFKDGVFRRRADVEGKRNLDGYQAIW
                     EHVNGRPMVYPKPRYPQPIFMDPANYDWVPVEGAPGVAEKLLGVFTERRSVAGLFKLD
                     AGASYAARGKGVYVVTRGAGSVGGESLRPLTTLYLGPGETATFTAREETELLHFGLPN
                     LADLRAPVRAAAPAEAAE"
     gene            1910..>2262
                     /locus_tag="VEL04_06700"
     CDS             1910..>2262
                     /locus_tag="VEL04_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012357009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYT48092.1"
                     /translation="MTAAALVAAAQAFAQDDAARRYPNRAVKVIVSAPPGGGLDIAAR
                     VIADRLAKMWGQPFVVENRPGASGNLGAEAVAQAEPDGYTLLAAQPGPLTTNVVMYKK
                     LNFDPAAFEPLAIMTS"
BASE COUNT          337 a          791 c          758 g          376 t
ORIGIN      
        1 gcatggtcat gcaggtgctc ctgatcgcgg tccagaacag cgtcgactac gagcatctcg
       61 gcgtggccac gtccggaacg atgctgttcc gctcgctggg cggcgcgctc ggcgtcgcgc
      121 tgttcggcgc catcttcgcg aaccgcctgc acgcgcagct cgccggcccc ggcatggatt
      181 tcctggccac ggtcattccg gcggcggtca agggcctgcc gcccgacctg catcaggaat
      241 acatcgccgc ggtgatggcg gcgctgcggc cggtcttcct ggcggcggcc ggcatttcgg
      301 ccttgggctt cgtgctgacc tttctgctgc gcgagtaccc gctgcgcgag ggcgcgcccg
      361 ccgagggcgt gggcgagagc ttcgcgatgc cgcgtgacgc gacctcgttg gaggagctcg
      421 agcgcatcat cacgctgctg gtggcgcgcg aaaatcgctg gcgcctctat gccgatctcg
      481 cccggcgcgc ggccatcgaa ctgcccgcgc ctgaactgtg gatgctggcg cggctgggcg
      541 agcgcgcgcc gatcacgctc acctcgctca gcgccgagct gaagattccg cagccacagc
      601 tcgcatctcc cttgcacgcg ctgtgcgagc gcggcatcgc ggagaaaaac gccaacggag
      661 agcttgagct cacgtcaacc ggcgtcgcga tgcgcgatcg catcgtcacc gcgcggcgca
      721 agggtctcgc cgacatgctc gcgcgctggc ggccggaaga acatcccgac gtgctggctt
      781 tgatcgagcg catggcgcag gcgctcacca gcgacctgcc ggcgccgaag gcggcgtagc
      841 aggagtcttc agcggaccca gatcgccccg ccaccaatga gtcgcgtgca gtctgctatt
      901 ccgccgcttc cgccggcgcc gcagcgcgca ccggcgcgcg caggtccgcg agattcggca
      961 gtccgaaatg caggagctcg gtctcctcgc gcgccgtgaa tgtcgccgtt tcgcccggcc
     1021 cgagataaag cgtggtgaga ggacgcagcg actcgccgcc gacgctgcct gcgccgcgcg
     1081 tgaccacgta gacgcccttg ccgcgcgcgg cgtagctcgc gccggcgtcg agcttgaaca
     1141 accctgctac gctgcgccgc tcggtgaaga cgccgagcag cttctcggcg acgccgggag
     1201 cgccttcgac cggcacccag tcatagttcg ccgggtccat gaagatcggc tgcggatacc
     1261 gcggcttggg atagaccatg ggcctgccgt tcacgtgttc ccagatcgcc tggtagccgt
     1321 cgagattgcg cttgccctcg acgtcggcgc gccggcgaaa gacgccgtcc ttgaactcgc
     1381 cctccgctct gagttcgcgc gttccggcct gcacctcggc ttgcgagaga tagccgctgc
     1441 cgctcgcgcc gccgaactgc agcacgatgg tcttgcaggc gatgtcgggg tcctggctct
     1501 gggggccgta gtagaccgcc tccgggaaat agccgaccat gccggcgacg agcttgccgt
     1561 cgcggccata gtcgagcacg ccctcgagct ggtagcggat ctgctcgaaa ttgtgccggt
     1621 ggcggggcga gttgaaatcg ccgcccatct ggccgaacga gagctggaaa ttgtcgagcg
     1681 cgcccggctc gccttccacc atgatgcggc cgtgaaatgt gccgccgcga tgctcgaggc
     1741 cgcgctttag cgggatctcg tcggtttgca cgatcttcat cgtttcctcc ttcgcccgcg
     1801 gcccgctcgg cccgcggacc gagacaattc atcccatgga gcatagcctg aggtcgcggt
     1861 agttggcagt ccccgaggtc gatcgatgcg gagaattttg cgcctcgttg tgacggcggc
     1921 cgcgctggtg gcggcggcgc aagccttcgc tcaagacgac gcagcgaggc gctatcccaa
     1981 ccgcgccgtc aaagtcatcg tgtcggctcc gcccggcggc ggcctcgaca tcgccgcacg
     2041 cgtgatcgca gatcggctgg cgaaaatgtg gggccagccg ttcgtggtgg agaaccgccc
     2101 gggcgccagc ggcaatctcg gcgccgaggc ggtcgcccag gccgagcccg acggctacac
     2161 cttgctcgcg gcgcagccgg ggccgctgac caccaacgtc gtcatgtaca agaagctcaa
     2221 cttcgacccc gctgccttcg agccgctcgc aatcatgacc tc
//
LOCUS       DASPXW010000294         2595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1094518, whole genome shotgun sequence.
ACCESSION   DASPXW010000294 DASPXW010000000
VERSION     DASPXW010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094518"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="VEL04_06705"
     CDS             <1..945
                     /locus_tag="VEL04_06705"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HYT48093.1"
                     /translation="WAASVQAEDQVRVSRLGVGEVRRQIAIATALAYLAVIAQKRLVD
                     VNALALENAQAHVDYARARVEAGAGSKLNELRASQELATDEVLLEAARLAERRAQEAL
                     GVLLAADAPVDAAGEPVFEVPPEARATAAEPTWMSARADVRLFSAQVDAANRIVRDSW
                     KDWVPGATASFEPQALAPAGLFQPARTWRGFMIVDVPIFDAGSRRAVKRQREVALDTA
                     RIQLTDVQLRAKAELRTAQAAVDSTERALEHARVAAQHAADVLRITDLAFRAGATTNI
                     ELIDAQRRARDADTAVAQAEDRVRQARLDLLVAVGKFP"
     gene            966..1796
                     /gene="amrB"
                     /locus_tag="VEL04_06710"
     CDS             966..1796
                     /gene="amrB"
                     /locus_tag="VEL04_06710"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HYT48094.1"
                     /translation="MLRRAAVAGSWYPGSPERLSAELTAHLQRADQATSTDAPPTADL
                     VGLIAPHAGLMYSGPVAAYAYRLLRDRAFDLTVLVGPSHFVPFEGVSVWSSGAFETPF
                     GKLLVDDRATAEVVASCPVVKDLRSAHEREHSLEMQLPFLKLLAPDTAILPLVMGQQT
                     RATVFALADALATVAQERRTLLVASSDLSHYQHAAVAARLDQQVIDRVEEMDDDGLMG
                     LLEKRPDHACGGGPMVSVLRAAKAVGARSCEVLRYADSGDVSGDKSAVVGYLAAAVWK
                     "
     gene            1902..2456
                     /gene="amrA"
                     /locus_tag="VEL04_06715"
     CDS             1902..2456
                     /gene="amrA"
                     /locus_tag="VEL04_06715"
                     /inference="COORDINATES: protein motif:HMM:TIGR04335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HYT48095.1"
                     /translation="MPTDPTIQLQLLTLARAALEARVRSLALPSVPLEIEVDGFGVFV
                     TIYHRGDLRGCLGSLQCQNAIVSAVARLAASVARDDYRFSPLMPHELAATTIDLSVLS
                     PPEEICGPMTIEIGRHGLIVEQGSQRGLLLPQVATEHGWDGETFLAHTCRKAGLPTTA
                     WLNGATVYRFEAEVFGEGTRPKRK"
BASE COUNT          434 a          834 c          868 g          459 t
ORIGIN      
        1 tgggcagcga gcgtccaggc ggaagatcag gtgcgtgtgt cgcggctggg cgtcggcgaa
       61 gtccgtcgcc aaatcgcgat tgcgacggca ctagcgtatc ttgcggtcat cgcgcagaag
      121 cgcctcgtcg acgtcaacgc cctcgcgctc gagaacgcgc aggcacatgt ggattacgcg
      181 cgcgcacgtg tcgaagcggg tgcaggcagc aaattgaacg agctcagagc gtcgcaggag
      241 ctcgcgaccg acgaagtgct cctcgaagcg gcccgcctcg cggagcggcg tgcgcaggag
      301 gcgctcggcg tgctgctggc cgccgacgct cccgtcgatg cagccggaga gccggtgttc
      361 gaagtgccgc cggaagcgcg tgctaccgcc gccgagccga catggatgtc cgcgcgagcc
      421 gacgtccgat tgttttcggc ccaggtcgat gccgccaatc gcatcgtgcg cgacagctgg
      481 aaagactggg tgcccggtgc gaccgcctcg ttcgagccgc aggcgctggc gcctgccggc
      541 ttgttccagc cggcacggac gtggcgcggt tttatgattg tggacgtccc gattttcgat
      601 gccgggtcga gacgcgctgt caaacgccag cgcgaagtcg cgctcgatac cgcgcgcatc
      661 cagttgacgg acgtgcagct tcgagccaag gccgagctcc gcaccgcgca ggccgccgtc
      721 gacagtaccg agcgtgcact cgaacacgcg cgggtcgcag cgcagcacgc cgcggacgtg
      781 ttgcggatca ccgatctcgc gtttcgcgcc ggcgcgacga cgaacatcga gctcattgac
      841 gcgcagcggc gcgcccgcga cgccgacacc gccgtggcgc aggccgaaga ccgtgttcgt
      901 caggcgagac tcgatctgct ggttgcggtg gggaagtttc cgtgacgcag gcgtaaactg
      961 gcgtcatgtt gcggcgggca gcggtcgccg gcagctggta tccgggttcc cccgagcgcc
     1021 tgagcgcgga gctcaccgcc catttgcagc gcgcggacca ggcgacgtct acagacgcac
     1081 cgccaacggc agatctcgtc ggtctcattg cgcctcatgc cggactgatg tactcgggcc
     1141 cggtcgcggc ctacgcgtat cgacttctgc gcgaccgcgc attcgatctc accgttctcg
     1201 tcgggccttc gcatttcgtt ccgttcgagg gcgtctccgt ctggtcatcg ggtgcgttcg
     1261 agactccgtt cggcaaactg ctcgtcgacg accgggcgac ggcagaggtc gtcgcgagct
     1321 gtccagtcgt caaggatctt cggtcggcgc acgaacgtga gcactcgctc gagatgcagc
     1381 ttcctttcct gaaactgctt gcgccagaca cggcaatact gccgcttgtg atgggtcagc
     1441 agacgcgcgc gaccgtgttc gctttggccg atgcgctggc tacggttgcg caggaacgcc
     1501 gtacgttgct cgtcgcgagc agcgatctat cgcactacca acatgcggcg gttgctgcgc
     1561 gcctcgatca acaggtgatc gaccgcgtag aggagatgga cgatgacggc ctgatgggac
     1621 tcctcgagaa gcgccccgat cacgcctgcg gcggaggccc catggtgtcg gtgctccgcg
     1681 ccgcgaaggc ggtgggcgcc aggagctgtg aggtgcttcg gtatgctgat tcaggcgacg
     1741 tgtcaggcga taagagcgca gttgtcggct atctcgccgc ggccgtttgg aaatagccaa
     1801 gtgccgagcc aagtgccaag tgccgagtgc caagtgccaa gtgccgagtg ccgttcagac
     1861 ccaagaccca agacccaaga ctgaccaaat gctgagggcc cgtgccgact gatccaacca
     1921 tccagcttca gttgctgaca cttgcgcgcg cggcgctcga ggcgcgcgtg cgcagtctcg
     1981 cgttaccgtc cgtgccactg gagatcgagg tcgacggatt tggtgtgttc gtcacgatct
     2041 accatcgcgg cgacctgcgc ggctgtctcg gttcgctcca gtgccaaaac gcgatcgtat
     2101 ccgccgtcgc acggcttgcc gcatcggtgg cgcgcgacga ctaccgcttt tcgccgctga
     2161 tgccgcacga gctcgccgcg acgacaatcg atctctccgt gttgtcgccg ccagaggaga
     2221 tttgtggtcc catgacgatc gaaataggcc gccacggcct cattgtggag cagggctctc
     2281 aacgcggcct gcttctgccg caagtggcga ccgagcacgg ctgggacggc gagacgttcc
     2341 tcgcgcacac gtgtcgcaag gcgggcctcc ccaccacggc ctggctcaat ggcgcgacgg
     2401 tttatcgttt cgaagcagag gtgtttggcg aaggcactcg tcccaagcgc aagtagccag
     2461 caccgcggag gaaaggcccg cgctacatgc agctctggga cgtagcgccg gactttgtgg
     2521 cggcttcagg caaccgctta tctcacgttg atcagttcga catcgaatac cagcgacgcg
     2581 ttcggcgcga tggtg
//
LOCUS       DASPXW010000295         2283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_86637, whole genome shotgun sequence.
ACCESSION   DASPXW010000295 DASPXW010000000
VERSION     DASPXW010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86637"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="VEL04_06720"
     CDS             complement(<1..914)
                     /locus_tag="VEL04_06720"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HYT48096.1"
                     /translation="MLERIGKYEIKRVLGKGATGTVYLAVDPFHNSEVALKVMEKLSS
                     DPEKARRQLRFFQNEAALAGKLRHPHIVSIFDAGVEDITGGEALRYLVMEVVNGGALT
                     KYCDPAALLDPARIVGIVYKCCKALEFANSIGVVHRDIKPANILVRDDYDIKVSDFGA
                     AQLARSEITQVTGVGSPAYMSPEQVKGEEIDFRTDMFSLGGVLYHLLTGARPFDGGNA
                     YQLVENILNGAPPPPSRVRPEVPAALDDIVLRALGKARDDRYATWNEFAEALAKVLGQ
                     QRRAPELSEAEKFGASRRLAFFKRFSDV"
     gene            complement(916..1599)
                     /locus_tag="VEL04_06725"
     CDS             complement(916..1599)
                     /locus_tag="VEL04_06725"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HYT48097.1"
                     /translation="MITLWELKGRDDRRYSLFSWRTRMALRHKDLPFEAKPVCMSDKA
                     AIAFSGGKTVPVIRDGESVIRDSWKIAEHLEERYGDKPSLFGGAIGRGVTQTFNTWVD
                     RTLVAAMMPVIVADIHERVDPADDAFFRQMFEGFLKSTLEQARSRRAEFAARLGRTLE
                     PMQAALKRQPFVSGATPAYGDYILFSVFQWARVMSPQELLGPEDPLAKWRHRLLDLYD
                     GFARNVPTA"
     gene            complement(1596..>2283)
                     /locus_tag="VEL04_06730"
     CDS             complement(1596..>2283)
                     /locus_tag="VEL04_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48098.1"
                     /translation="YAGEAGFDMLEIHMAHGYLLASFISPLTNQRKDSYGGSIENRMR
                     FPLEVWRACRRLWPEEKPMSVRISATDWAPGGLPGADLIALTRMLKEAGCDLIDCSTG
                     QTVPQQKPVYGRMYQAPYADWVRNEVGIATMAVGAVTTPDQVNTLLAAGKADLVALAR
                     PHLSNPYFTLQASAWYQHHRQHWPLQYLAGRDQAFRLAARERAELTDVRLRARPASHE
                     IKDAGVKKAA"
BASE COUNT          363 a          738 c          773 g          409 t
ORIGIN      
        1 tcgacgtcgc tgaagcgctt gaagaaggcg agacggcgcg acgcgccgaa cttctcggct
       61 tccgagagct cgggggcgcg gcgctgctgg ccgagcacct tggcgagcgc ctcggcgaac
      121 tcgttccagg tggcgtagcg gtcatcgcgc gccttgccca gagcgcgcag cacgatgtcg
      181 tcgagcgccg ccggaacttc gggccgcacg cgcgagggcg gcggcggcgc gccgttcagg
      241 atgttttcga cgagctggta ggcgttgccg ccgtcgaagg ggcgcgcgcc ggtgagcagg
      301 tgatagagca cgccgccgag cgagaacatg tcggtgcgaa agtcgatctc ctcgcccttc
      361 acctgctcgg gcgacatgta ggcgggggag cccacccccg tcacctgcgt gatttcgctg
      421 cgcgcgagct gcgcggcgcc gaagtccgag accttgatgt cgtagtcgtc gcgcaccagg
      481 atgttcgccg gcttgatgtc gcgatgcacg acgccgatgc tgttggcgaa ctcgagcgcc
      541 ttgcagcact tgtagacgat gccgacgatg cgcgccgggt cgagcagcgc ggcgggatcg
      601 cagtacttgg tgagcgcgcc gccgttcacc acctccatca ccaggtagcg cagcgcttcg
      661 ccgccggtga tatcttcgac gccggcgtcg aagatgctga cgatgtgcgg gtggcgcagc
      721 ttgccggcga gcgccgcctc gttctggaaa aagcgcagct ggcgccgtgc cttttcaggg
      781 tcggacgaca gcttttccat caccttcagc gccacttcgc tgttgtggaa cgggtccaca
      841 gcgaggtaca ccgtgccggt agcgcccttg ccgagcaccc gcttgatctc gtacttgccg
      901 atgcgttcga gcatgtcagg cggtcggcac gttgcgtgca aagccatcgt acaggtcgag
      961 caggcggtgg cgccatttgg cgagcggatc ttccggcccg agcagctcct gcgggctcat
     1021 cacgcgcgcc cactggaaca ccgagaacag gatgtaatcg ccataggccg gtgttgcgcc
     1081 ggagacgaac ggctggcgct tgagcgcggc ctgcatcggc tccagggtcc tgccaagacg
     1141 agcggcaaac tcggccctgc ggctgcgcgc ctgctcgagc gtcgacttga ggaagccttc
     1201 gaacatctgg cggaagaagg cgtcgtccgc cgggtcgacg cgctcatgga tgtcggccac
     1261 gatcaccggc atcattgccg cgaccagcgt gcggtccacc caggtgttga aggtctgggt
     1321 gacgccacgg ccgatggcgc cgccgaagag cgaaggcttg tcgccatagc gctcttccag
     1381 gtgttcggcg atcttccagg aatcccggat cacgctctcg ccgtcgcgga tcaccggcac
     1441 ggtcttgccg ccggaaaagg cgatcgccgc cttgtcgctc atgcaaacgg gtttcgcttc
     1501 aaaaggcaga tctttgtggc gcagcgccat ccgggtgcgc caggagaaga ggctgtagcg
     1561 ccggtcgtcc ctgcccttca gttcccaaag ggtaatcaag ctgccttctt gactcctgcg
     1621 tccttgatct catggctcgc gggcctcgcg cgcaatcgca cgtcggtcag ctcggcgcgc
     1681 tcgcgcgcgg cgagccggaa agcctggtcg cgccccgcaa ggtattgaag cggccagtgc
     1741 tgcctatgat gctgatacca cgccgaggcc tgcagcgtga agtacggatt gctcaggtgc
     1801 ggtcgcgcga gcgccacgag atcggccttt ccggccgcca gcagggtatt cacctggtcc
     1861 ggggtcgtga ccgcgcccac cgccatggtg gcgatgccga cctcgttgcg cacccagtcg
     1921 gcgtagggcg cctggtacat gcggccgtac accggtttct gctgcggcac ggtctggccg
     1981 gtcgagcagt cgatgaggtc gcagcccgct tccttgagca tgcgggtaag cgcgatcagg
     2041 tccgcgccgg ggagcccccc cggcgcccag tcggttgcgg agatgcgcac cgacatcggt
     2101 ttttcttccg gccagagcct cctgcacgcg cgccagactt ccagcgggaa gcgcatgcgg
     2161 ttctcgatcg accctccgta ggagtctttt ctttgattgg tcaagggaga aataaaactg
     2221 gcaagcaggt agccgtgcgc catgtggatt tccagcatgt cgaacccggc ttcgcccgcg
     2281 tag
//
LOCUS       DASPXW010000296         2332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1094790, whole genome shotgun sequence.
ACCESSION   DASPXW010000296 DASPXW010000000
VERSION     DASPXW010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094790"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="VEL04_06735"
     CDS             complement(<1..490)
                     /locus_tag="VEL04_06735"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48099.1"
                     /translation="MNRILTTHCGSLPRPHDLLDLMKAKVNGQPYDAGAYERRVRKAV
                     ADAVRKQADTGIDILSDGEQSKPGFFTYVKERLAGFEARPGAHVAFFEAERAAFPEYY
                     KEYFARAMLGGSVTPVVPMVCTGPVSYRGANALARDIETLKAALSGVQAEAVFMPAVA
                     PSG"
     gene            complement(501..1211)
                     /locus_tag="VEL04_06740"
     CDS             complement(501..1211)
                     /locus_tag="VEL04_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT48100.1"
                     /translation="MAEPLLELKGVRAGYGDAVVLDDISLAIPDNGSLAVLGRNGVGK
                     STLLLTIMGFTRIHAGKILWRGRDMTGVPPHLRAKRGIGWVAQEREIFPSLTLEENLT
                     VAARPGRWNLAAVYRLFPRLRERKTNLGNQLSGGEQQMLAIARALMTNPRLLLLDEPL
                     EGLAPIIVEELAVSLKKMMQIHGTAAILVEQHVEIALSLTEDAIVIERGAIAHRGGSR
                     DLPSDIETLERLVGLKVT"
     gene            complement(1204..1902)
                     /locus_tag="VEL04_06745"
     CDS             complement(1204..1902)
                     /locus_tag="VEL04_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT48101.1"
                     /translation="MAQDIELALPQGVRYALIGPNGAGKTTLINLITGMLRPDGGQIL
                     LGADDITSLAPAARVKRGLARTFQINSLFPHLTALEAVTLAVLERGNHAATWWRPLPA
                     YRDAIEEAYSLIHSLNLETACNRTTRELAYGQQRLLEIALALATRPRVLLLDEPAAGV
                     PKDESAALFAAIAGLSRDLTVLFIEHDMNVVFRFASRVIVMVGGRILVEGTPEEIADH
                     AQVREVYLGKAKHG"
     gene            complement(1926..>2332)
                     /locus_tag="VEL04_06750"
     CDS             complement(1926..>2332)
                     /locus_tag="VEL04_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYT48102.1"
                     /translation="SPFGLALRGIRENALRMPAIGAPSRAHLRKVYTIAAVIAGIAGA
                     LLAQTTETVSLEVLSFGRSADVLVILILGGAGRLYGGIVGAILYMVARDQFSGMNPQY
                     WYFWIGLLLVAVVMFLPNGVLGGVAKFSQRKS"
BASE COUNT          384 a          775 c          773 g          400 t
ORIGIN      
        1 cgccgctcgg cgccacggcg ggcatgaaga ccgcctcggc ttgcacgccg cttagcgctg
       61 ccttgagcgt ctcgatgtcg cgcgcgagcg cgttggcgcc gcggtagctt accggcccgg
      121 tgcagaccat cggcaccacc ggcgtcaccg agccgccgag catcgcgcgg gcgaaatatt
      181 ccttgtagta ctcgggaaat gcggcgcgct cggcctcgaa gaacgccaca tgcgcgccgg
      241 gcctcgcctc gaagcccgcc agccgctcct tcacgtaggt gaagaagccc ggcttggact
      301 gctcgccgtc gctcaggatg tcgatgccgg tgtcggcctg cttgcgcacg gcgtccgcga
      361 ccgccttgcg cacgcgccgc tcgtaggcgc cggcgtcgta cggctgcccg ttcaccttgg
      421 ccttcatcag gtcgagcagg tcgtggggac gcggcaggct gccgcagtgc gtggtcaata
      481 tgcggttcat gaaatcatca tcaggttacc ttcaaaccga ccagccgctc cagcgtttcg
      541 atgtcgctcg gcaggtcgcg cgagccgccg cggtgcgcga tcgcgccgcg ctcgatgacg
      601 atagcatcct cggtgagcga gagcgcgatc tcgacgtgct gttcaacgag gatcgcggcc
      661 gtgccgtgga tttgcatcat ttttttcaat gaaaccgcca attcttcgac gatgatcggc
      721 gccaggccct cgagcggctc gtcgagcagc aggagccgcg gattggtcat cagcgcgcgc
      781 gcgatggcga gcatctgctg ctcgccgccg gagagctggt tgccgagatt cgtctttcgc
      841 tcccgcaatc ttggaaacaa tctgtagacg gcagccaggt tccagcgccc gggccgcgcc
      901 gccaccgtca gattctcctc cagggtaagg gacggaaaga tctcgcgttc ctgcgccacc
      961 cagccgatgc cgcgcttcgc gcgcaggtgc ggagggactc cagtcatatc ccggccgcgc
     1021 cacaggattt tcccggcatg aattctggtg aaacccatga tggtgaggag cagcgtgctc
     1081 ttgccgacgc cgttgcggcc gagcaccgcc aggctgccgt tgtccggtat cgcaagggaa
     1141 atatcgtcga gcaccacggc atcgccgtag cccgcgcgca cgcctttcag ctcgagcagc
     1201 ggctcagcca tgcttcgcct tcccgaggta cacctcgcgc acctgcgcgt ggtcggcgat
     1261 ctcctccggc gtaccttcga ccaggatgcg gccgccgacc atgacgatga cgcggctcgc
     1321 gaagcggaac accacgttca tgtcgtgctc gatgaacagc accgtgaggt cgcgcgacaa
     1381 gccggcgatg gcagcgaaca gggcggcgct ctcgtccttg ggcacgcccg ctgccggctc
     1441 gtcgagcagc agcacgcgcg ggcgcgtggc gagcgcgagc gcgatctcga gcagccgctg
     1501 ctggccgtat gccagctctc gggtcgtgcg attgcacgct gtttccagat tcaatgaatg
     1561 gatgagacta taggcctctt cgatcgcatc ccggtacgcg gggagtggcc gccaccaggt
     1621 tgcggcatgg tttccacgct caagcactgc aagcgttact gcttccagcg cggtgagatg
     1681 gggaaagagg ctgttgatct ggaaggtgcg cgcgaggccg cgcttgacgc gcgcggcggg
     1741 cgcgagcgaa gtgatgtcgt ccgcgccgag caggatctgg ccgccgtccg ggcgaagcat
     1801 gccggtgatg aggttgatga gcgtcgtctt gccggcgccg ttgggcccga tcagcgcgta
     1861 gcgcaccccc tgcggcagcg cgagctcgat gtcctgcgcc acgaccagcg agccgaactt
     1921 cttgttcagc tttttcgttg agagaatttt gcgacgcctc ccagcacgcc gttgggcagg
     1981 aacatcacca cggcgacgag cagcaagccg atccagaaat accagtattg cgggttcatg
     2041 cccgagaact ggtcgcgcgc caccatgtag aggatcgcgc cgacgatgcc gccgtaaagc
     2101 cggcccgcgc cgccgaggat caggatcacc agcacgtcgg ccgagcgccc gaacgacagc
     2161 acttccagcg agacggtttc ggttgtctgc gcgagcagcg cgcccgcgat gccggcgatc
     2221 actgcggcga tggtgtagac cttgcgcagg tgcgctcggc tcggcgcgcc gatcgccggc
     2281 atgcgcagcg cgttctcgcg gatgccgcgc aacgccaggc cgaagggcga at
//
LOCUS       DASPXW010000297         3717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1595545, whole genome shotgun sequence.
ACCESSION   DASPXW010000297 DASPXW010000000
VERSION     DASPXW010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1595545"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="VEL04_06755"
     CDS             complement(<1..805)
                     /locus_tag="VEL04_06755"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HYT48103.1"
                     /translation="MSERFVPPVRFSRLLLKVNAFLLLALLLVAAFMGLVAYKQGWFV
                     HQTVIHFVTPNALGINKGMPVKLYGFTVGSVSTMELSREGVDVRLSIMSEYMPRIPKG
                     SQARHAREAGVVGASVIELHPGPGSQPLAEGERIDFQPGRGISEIIDDIRRQAVPAFD
                     EMKQVLSKLKGSGEDVSVALSTFRREIERVPETHRALRKLLENADREMQAAQRATEIV
                     ERAVPMLAGKLATVIDSLDAAAVQLRRTGEEAQGTLQRARPLLDRGETAA"
     gene            complement(802..1437)
                     /locus_tag="VEL04_06760"
     CDS             complement(802..1437)
                     /locus_tag="VEL04_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48104.1"
                     /translation="MTAPQAASAGRAQMALRYARKTAQREVTLAVPRGSVRHVQLPDQ
                     GSKAHLVTAVLKARCEDGEELELLGEPVRSLKPAARERLRARVGALSPVIGLITSLNA
                     WENISLAAAYHGKPPLAEVARITHEVLRTFGAEPRSFLNRVPDELGMLERKIAAFVRL
                     LIAAPELAIIDALDEGLSREECARVAGFEKEYRGRHPSGTLLFIDTREGDS"
     gene            complement(1434..2210)
                     /locus_tag="VEL04_06765"
     CDS             complement(1434..2210)
                     /locus_tag="VEL04_06765"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYT48105.1"
                     /translation="MLRPLHEIEGGLRLSLNVAGRLGQLRRPPVLESFLRQVYFTGVT
                     AATGVVLRASGLGVLIIAITMDVLDADVDLAVKILLLVVFREVGPLAAAAVVILRSGT
                     AMSAEMAIMRISGQTRTLRYLGINLYDYLVVPRVAGVMLAILVLTLYIQALAVFGGLV
                     LSPFIIQASFMELTGRLFELFTPVDFFYSTVKSLLFGFAIAACCCYHGLNPPDLTQNA
                     VPKVVTRAVTQCAMLVLTINAVFAYVVFGILFFGLVTAKV"
     gene            2319..2570
                     /gene="rpsP"
                     /locus_tag="VEL04_06770"
     CDS             2319..2570
                     /gene="rpsP"
                     /locus_tag="VEL04_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HYT48106.1"
                     /translation="MVVIRLQRGGANKRPFFHLVVADSRRAASGKFLERVGFYDPKAP
                     AGREALRVNLERVKHWTGQGAQLSGTAARLVKQFAKKAA"
     gene            2584..3042
                     /gene="rimM"
                     /locus_tag="VEL04_06775"
     CDS             2584..3042
                     /gene="rimM"
                     /locus_tag="VEL04_06775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HYT48107.1"
                     /translation="MGRVAGSYGVRGWIKVQHPEAALPACSKWWIGGMEYAVEEAKAH
                     SGTLLAKLAGIGSREAALKLKGATVSVRRAALPEAGEGHYYLADLVGLEVVNASGVAL
                     GFVKRMFTNGAQDVMEVAGDRTRLLPWVAAVVKRVDLSARRIEVEWQADW"
     gene            3056..>3717
                     /gene="trmD"
                     /locus_tag="VEL04_06780"
     CDS             3056..>3717
                     /gene="trmD"
                     /locus_tag="VEL04_06780"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HYT48108.1"
                     /translation="MRFDVVTLFPEMLAALTQSGITGRALEAGLWRLQAWNPRDFTSD
                     NYRTVDDRPYGGGPGMVMLAEPLEKALDAAKFAGGGRVVYLTPQGSKLEHRKVMELSK
                     ESALTLLCGRYEGVDERLLARRVDEELSLGDFVLSGGELAAMALIDAVVRQLPGALGD
                     EHSAQEESFAAGLLDCPQYTRPELYAGAKVPEVLLSGHHGHIRRWRLKQALGRTWLRR
                     PDL"
BASE COUNT          631 a         1197 c         1303 g          586 t
ORIGIN      
        1 gtgcggccgt ctcgccgcgg tcgagcagcg gcctggcgcg ctgcagcgtg ccctgcgcct
       61 cctcgccggt gcggcgcagc tggaccgcgg cggcatccag cgaatcgatc accgtcgcca
      121 gcttgccggc cagcatcggc acggcgcgct cgacgatctc ggtggcgcgc tgcgccgcct
      181 gcatctcacg gtcggcattc tcgagcagct tgcgcagcgc gcggtgcgtc tcgggcacgc
      241 gctcgatctc gcggcgaaat gtcgacagcg cgacgctgac gtcttctcca gagcccttga
      301 gcttcgaaag cacctgtttc atctcgtcga aggccggcac cgcctgccgc ctgatgtcgt
      361 cgatgatctc ggagattccc cggcccggct ggaagtcgat gcgctcgccc tcggcgaggg
      421 gctgggagcc cggcccggga tggagctcga tcacgctcgc cccgaccacg cccgcctcgc
      481 gcgcgtgccg tgcctgcgat cccttcggaa tgcgcggcat gtattcgctc atgatcgaga
      541 gccgcacgtc gacgccctcc cgcgacagct ccatggtgct gaccgagccc accgtgaagc
      601 cgtagagctt gaccggcatg cccttgttga tgccgagcgc attcggcgtc acgaaatgga
      661 tcaccgtctg gtggacgaac cagccctgct tgtaggccac caggcccatg aacgccgcca
      721 ccagcaggag cgcgagcaac aggaacgcgt tcaccttgag gagcaggcgc gagaatctca
      781 ccggcggcac aaatctttcg ctcatgaatc accctccctc gtatcgatga acagcagcgt
      841 ccccgacgga tgccgcccgc ggtattcctt ctcgaagccg gcgacgcgcg cgcattcctc
      901 gcggctcagg ccctcgtcga gcgcatcgat gatggcgagc tccggcgcgg cgatgagcag
      961 gcgcacgaat gccgcgattt tccgctccag catgccgagc tcgtcgggca cccggttgag
     1021 gaacgagcgc ggctcggcgc cgaaggtcct cagcacctcg tgcgtgatgc gggcgacctc
     1081 tgcgagcggt ggcttgccgt ggtacgccgc cgcgagcgag atgttctccc acgcattgag
     1141 gctggtgatg agcccgatga cgggcgacag cgcgccgacg cgcgcgcgca accgctcccg
     1201 ggcagccggc ttgaggctgc gcaccggttc gccgagcagc tcgagctcct cgccgtcctc
     1261 gcagcgggct ttgagcaccg cggtgacgag gtgcgccttt gagccctggt cgggcagttg
     1321 cacgtgcctg acgctgccgc gcggcaccgc gagggtgacc tcgcgttgcg cggtcttgcg
     1381 cgcataccgc agcgccattt gcgcccggcc ggcagatgcc gcttgcggcg cggtcatacc
     1441 tttgccgtca ccagaccgaa gaacaggatg ccgaagacca cgtatgcgaa caccgcgttg
     1501 atcgtcagca ccagcatcgc gcactgcgtg actgcccggg tcacgacctt cggcaccgcg
     1561 ttctgtgtca ggtcgggtgg attgaggccg tgatagcagc agcacgcggc aatggcgaaa
     1621 ccgaacagca ggctcttgac cgtcgagtag aaaaagtcga ccggcgtaaa cagctcgaag
     1681 aggcgcccgg tgagctccat gaacgacgcc tggatgatga acgggctcag caccagcccg
     1741 ccgaacaccg ccagcgcctg gatgtagagc gtgagcacta ggatggcgag catcacccct
     1801 gccacgcgcg gcacgacgag atagtcgtac aggttgatgc cgagatagcg cagcgtgcgc
     1861 gtctgcccgg agatgcgcat gatcgccatc tcggcggaca tggcggtgcc gctgcgcagg
     1921 atgacgaccg ctgccgcggc gagcgggccg acctcgcgga acaccaccag cagcagaatt
     1981 tttaccgcaa gatcgacgtc cgcgtcgagc acgtccatcg taatcgcgat gatcagcacg
     2041 cccagacccg aggcgcgcag caccacgccg gtcgcggccg tgacgccggt gaaatagacc
     2101 tgccgcagga acgattccag aaccgggggc ctgcgcagct ggccgaggcg gccggccaca
     2161 ttgagcgaca gtctgagacc accctcgatt tcgtgaagcg gacgcagcat ccaacctccc
     2221 cgcagtattt tgcaccagca gacggtgcac cggcgttcca aaacgccgat ttctcccgta
     2281 taatccgcgc cttttcgcag tttgccgggg gtttcccaat ggtggtgatt cgactgcagc
     2341 gcggcggcgc caacaagcgg ccgtttttcc acctggtggt ggcggattca cgccgcgctg
     2401 cgagcggcaa gttcctcgag cgcgtcgggt tctacgatcc gaaagcgccc gcagggcgcg
     2461 aggcgctgcg cgtcaacttg gagcgcgtga agcactggac cggccagggc gcgcagctct
     2521 cgggcacggc ggcgcggctc gtcaagcaat ttgccaagaa ggccgcctga ggacctagtc
     2581 gagatgggcc gcgtcgccgg gtcatacggc gtgcgcggct ggatcaaggt gcagcacccg
     2641 gaagcggcgc tccccgcgtg cagcaaatgg tggatcggcg gcatggaata cgctgtcgag
     2701 gaagcgaagg cgcattcggg cacactgctc gcgaagctcg cgggaatcgg cagccgcgag
     2761 gcggcgctga agctgaaagg cgcgaccgtg tcggtgcggc gcgcggcgct gcccgaggcc
     2821 ggggaagggc actactacct tgccgacctg gtcggcctcg aggtggtgaa cgcgagtggc
     2881 gtcgcgctcg gcttcgtgaa gcggatgttt acgaacggcg cgcaggacgt gatggaagtg
     2941 gcgggcgaca ggacgcgatt gctgccttgg gttgccgcgg tggtgaaacg agtcgatttg
     3001 tcggcaagac gaatcgaggt ggagtggcag gcggactggt gaggcgggac gggtgatccg
     3061 cttcgatgtc gtgacgctgt ttcccgagat gctcgcggcg ctgacgcaga gcggcatcac
     3121 gggccgggcg ctcgaggccg ggctgtggcg gctgcaggcg tggaatccgc gcgatttcac
     3181 gagcgacaac taccgcacgg tggatgaccg tccgtacggc ggcgggccgg gcatggtgat
     3241 gctggcagag cccttggaaa aggcgctgga cgcggcgaaa ttcgccggcg gcggaagggt
     3301 ggtttacctg acgccgcaag gcagcaagct cgagcaccgc aaagtgatgg agctttcgaa
     3361 ggaaagcgcg ctgacgctgc tgtgcggccg ctatgaaggc gtcgacgaaa ggctgctggc
     3421 gcggcgcgtc gacgaagagc tgtcgctcgg cgatttcgtg ctttcgggcg gcgagctcgc
     3481 cgcgatggcg ctgatcgacg cggtggtgcg gcaattgccc ggggcgctgg gcgatgagca
     3541 ctcggcgcag gaagagtctt tcgcggccgg gctgctcgat tgccctcagt acacgcggcc
     3601 cgagctgtat gcgggcgcaa aagtgccgga ggtgctgctt tcggggcacc acgggcacat
     3661 ccggcgctgg cggctgaagc aggcgctggg gcggacctgg ctcagaaggc cggatct
//
LOCUS       DASPXW010000298         2272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1222300, whole genome shotgun sequence.
ACCESSION   DASPXW010000298 DASPXW010000000
VERSION     DASPXW010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222300"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="VEL04_06785"
     CDS             <1..590
                     /locus_tag="VEL04_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HYT48109.1"
                     /translation="WRIAYERQWDRNSWSVGAYGISTKITPAGQPVRASSDRFRDVAL
                     DSQYQFIGDEQIFSAQATYIKEHQSLDATFALGDSDNQSNDLKTLRVGGSYYLRRTYG
                     GALGYFSTTGSADAKRYLPGTDPASGPVIGFANGSPDSKGWIAELNYLPWQNVKLALQ
                     YVAYQKFNGAASNYDGLGRDASDNNTLYLLGWFNF"
     gene            731..1297
                     /locus_tag="VEL04_06790"
     CDS             731..1297
                     /locus_tag="VEL04_06790"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HYT48110.1"
                     /translation="MAPSLRAQEADVGKYASQVCASCHGPRGESVSPGFPKIAGQRAE
                     YLENQMKAFRDRTRADPMAQAYMWGMTSQLSDDAIKKLAVFYSGQKPPQGKAADPKLA
                     QAGKAIYEGGIPAAKVQACATCHGKNAEGNAIFPRLAGQHAEYLVKQLVLFKSSLREG
                     SNAPVMHNISTGMTFEQMEAVAAYLASK"
     gene            1313..1774
                     /locus_tag="VEL04_06795"
     CDS             1313..1774
                     /locus_tag="VEL04_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48111.1"
                     /translation="MSSRARNTNWRTSTPGHRLWFAAGCIAIALSTPAAGQDDELVRL
                     HQEAAALRQALERLEARIQALEGNAGAASASPGYSKGIDALASLKRGWSQVVPGLPED
                     KVEALLGKPEKILRIDNTPVWYYIYPEIGRGSVFFNGNGKVSSAQSPSLGW"
     gene            complement(1890..>2272)
                     /locus_tag="VEL04_06800"
     CDS             complement(1890..>2272)
                     /locus_tag="VEL04_06800"
                     /inference="COORDINATES: protein motif:HMM:NF024678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4070 domain-containing protein"
                     /protein_id="HYT48112.1"
                     /translation="REGRMLADSIGDNTVAVLNFVPRLDRWRLLAGYRELMRSLYAPE
                     NYYRRVRAFLATWRPRGPGGRLAWSDVRAFLRSLWTLGVSRRGRLAYWRLFWATLIVQ
                     PRKFRAAMELAIMGHHFRTVAERL"
BASE COUNT          453 a          731 c          732 g          356 t
ORIGIN      
        1 actggcgaat cgcctacgag cgccagtggg atcgaaactc atggtccgtc ggcgcctacg
       61 ggatcagcac gaagatcact cctgccggcc aacccgtccg cgcgtcctcc gatcgctttc
      121 gcgacgtcgc gctcgacagc cagtatcaat tcatcggtga cgagcaaatc ttttcggcac
      181 aagcaaccta catcaaggag caccagtccc tcgatgcgac ttttgcgctc ggcgactccg
      241 ataaccagag caacgacctc aagacgctcc gggtcggcgg cagctattac ctccgccgca
      301 cctacggcgg cgcgctgggc tatttctcga cgacgggcag cgcggatgcg aagaggtatc
      361 tgcccgggac cgatcccgcg tcgggacctg tcatcggttt cgccaacggc agccccgaca
      421 gcaagggctg gatcgccgag ctcaactacc tgccctggca gaacgtaaag ctcgcgctgc
      481 aatacgtcgc ctaccagaaa ttcaacggcg ccgcctcgaa ctacgacggt ctcggcaggg
      541 acgcgagcga caacaatact ctttacctgc tcggctggtt caatttctga agaggcgtcg
      601 aggcgcaacg ttcctgaatc gaatggccgg cggctcgatg aaccggagaa agccttccaa
      661 catgaggcag acgaatatga gaaccgatgt gattgttccg atttcgctcg tgacgatgac
      721 gatcgcgtgc atggctccgt ccctgcgggc gcaagaggcg gacgtcggca aatatgcttc
      781 ccaggtctgc gcgtcgtgcc acgggccgag gggagagagc gtctcgcccg ggttcccgaa
      841 gatcgccggc cagcgcgccg aatacctgga gaaccagatg aaggcgttcc gcgaccggac
      901 gcgcgccgat cccatggcgc aggcctacat gtggggaatg acctcgcagc tgagcgacga
      961 cgccatcaag aagcttgccg tcttttattc cggacagaag ccgcctcaag gcaaggcggc
     1021 ggatcccaag ctcgcgcagg cgggcaaagc catctacgag ggaggcattc ccgcggcaaa
     1081 agtccaggcc tgcgccacgt gtcacggcaa gaacgccgag ggcaacgcca tctttccgag
     1141 gcttgccggc cagcacgccg aatacctcgt caagcagctg gtcctgttca agagctcgct
     1201 gcgggaaggc agcaacgcgc cggtcatgca caacatcagc accggcatga ctttcgagca
     1261 gatggaggcc gtcgcggctt acctcgcgtc gaagtagcgg caggcgaaac acttgtcttc
     1321 cagagcgcgc aatacgaact ggcgcacgtc gactccgggg catcgcctct ggttcgcggc
     1381 gggctgcatc gccattgcgc tgtcgacgcc ggcggccggg caggacgacg agctcgtccg
     1441 ccttcatcag gaagcggcgg cgctgcggca ggcgctggag aggctcgagg cgaggatcca
     1501 ggcgctcgaa gggaacgcgg gagctgcgag cgcttctcca ggctactcca aaggcatcga
     1561 tgcgcttgct tcgctcaagc gcggctggtc gcaggtcgtt ccgggattac ccgaagataa
     1621 ggtcgaagcg ctgctcggca agccggagaa gatactgcgc atcgacaaca ctccggtgtg
     1681 gtactacatc tatccggaga tcggccgcgg ctcggtgttc tttaacggaa acggaaaggt
     1741 ctcgagcgcg caatcgccaa gcctcggctg gtgaaagatc gggaacagca accgggcgca
     1801 tgggattatc ggagtgcgcg tgccccaatc acatcgcgca gcgggccggg cagaacgtgg
     1861 gctggtccga ttcctttctg ctcatcgcct cacaagcgct cggccacggt gcggaagtgg
     1921 tggcccatga tggcgagctc catcgcggcg cggaatttgc gcggctgcac gatcagcgtc
     1981 gcccagaata gtcgccagta cgcgagccgc cctcgccgcg acacgcccag cgtccacagc
     2041 gagcgcagga acgcgcgcac gtcggaccag gcgagccgcc cgccgggtcc gcgcggccgc
     2101 caggtcgcga ggaaggcacg cacgcggcgg tagtagttct ccggcgcgta gagcgagcgc
     2161 atcagctcgc ggtagccggc gagcagccgc cagcgatcca ggcgcgggac gaagttgagc
     2221 acggccacag tgttgtcgcc gatgctgtcg gcgagcatgc gcccctcgcg cg
//
LOCUS       DASPXW010000299         3413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_839940, whole genome shotgun sequence.
ACCESSION   DASPXW010000299 DASPXW010000000
VERSION     DASPXW010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839940"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..731
                     /locus_tag="VEL04_06805"
     CDS             102..731
                     /locus_tag="VEL04_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005663243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HYT48113.1"
                     /translation="MSPTTKGEQTRAAILDEALSIASRLGLEGLTIGSLADATGMSKS
                     GLFAHFGSREDLQLAVLEHAAQRYGELVFVPVLKIERGLPRLRALFARWLDWTIASGL
                     PGGCIMISAANEYDDRPGPIRDAVIANQHRGNAITQKAVRLAIDEGHLRRDTDPEQIA
                     FEMLGIVLASHNHRRLLGDKETRKRALVAFDELIARHAAARKPLKAVSR"
     gene            745..1644
                     /locus_tag="VEL04_06810"
     CDS             745..1644
                     /locus_tag="VEL04_06810"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYT48114.1"
                     /translation="MPHSAALPRISTNVRFATARFLAPDLAGAWAERLFLTPPKRSDG
                     VATALDLIDGKASFIEHKGRQIAIWRWGSRDAPAVLLAHGWGGHAAQMRGFVFPLLAA
                     GYRVIAYDQPAHGISGGRLTGLPDFAEVMSEVAWHHGEVRAVIAHSLGGAAAALALAR
                     GLPLEKAVLVSPPADLVGYSRRFARWYWIPERARRSMQAAIEERYGVRWDELELSRLA
                     PRLSAPALVIHDRDDRMVSWTQGASFARLWPGARLLSTDGLGHRRILQDEKVTQAAAD
                     FISGRSSIASPAVPALPQPAPLY"
     gene            1661..2155
                     /locus_tag="VEL04_06815"
     CDS             1661..2155
                     /locus_tag="VEL04_06815"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HYT48115.1"
                     /translation="MEKRLRSLAITWSDPQELAAAGRTMAGIDFLRAILDGRLPLPPI
                     CELLGFRPVEVEPGRVVFEVLPGEQHYNPIGVVHGGLAMTLLDSAMGCSVQSRMPAGG
                     GYTTLEAKTNLVRAISSQTGKLRAVGKLINLGKRVATAEGRLEDLSGKLYAHASTTCL
                     VLAP"
     gene            complement(2152..2325)
                     /locus_tag="VEL04_06820"
     CDS             complement(2152..2325)
                     /locus_tag="VEL04_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase-like domain-containing protein"
                     /protein_id="HYT48116.1"
                     /translation="MKRGGKEALPALPPREPSPEECCGKGCTPCVFDTYAEALEKHRA
                     ALEAWRNLKIRKR"
     gene            complement(2322..>3413)
                     /locus_tag="VEL04_06825"
     CDS             complement(2322..>3413)
                     /locus_tag="VEL04_06825"
                     /inference="COORDINATES: protein motif:HMM:NF017775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYT48117.1"
                     /translation="LIGLDFNQQRFEADFRFSLVRLRENSEGVALYHGEGEELVRFRD
                     RFAAVIGNWWALMRKQKQLGWFTSFYGQLAIIFPYVVVSPRFFSGAMQMGGIFQTASA
                     FGQVQGALSWFISAYTQFATWKATVDRLIGFSASLERVRELKIEGERVEQAGNAVALE
                     DLQLNLPQGRPLLAAATLELKPREDVLVSGPSGAGKSTLFRALAGIWPYWKGRIRLPK
                     GARLLFLPQKPYLPIGSVKHAVCYPAETAMHSDEAVKEALRAVGLAQLADELERSENW
                     AQVLSGGEQQRLAFARALLYRPDWLFLDEATASLPDDAQQALYRLLKERLPDTTLVSI
                     GHRESLAEFHPRRLSWSQAGGAVPVLAPA"
BASE COUNT          569 a         1169 c         1126 g          549 t
ORIGIN      
        1 aggcacgccg gaaaaggtcg cagcgagcaa gcagagctac accgggcgcg cgttgcgccc
       61 gctcttgaca aatacgaacg ttcgtactat tatgccagcg catgagcccc accactaagg
      121 gcgagcagac ccgcgccgcg atcctcgacg aggcgctcag catcgcgagc cgcctcggcc
      181 tggaaggcct gaccatcggc agcctggcgg atgccaccgg catgtccaag tcgggactgt
      241 tcgcgcattt cggctcgcgc gaagacctgc agctcgccgt gctggagcac gctgcccagc
      301 gctacggcga gctggtgttc gtgccggtgc tgaagatcga gcgcggcctg cctcgcctgc
      361 gggcgttgtt cgcgcgctgg cttgactgga ccatcgcctc gggcctgccg ggaggctgca
      421 tcatgatctc ggcggccaat gagtacgacg accggccggg cccgatccgc gacgcggtga
      481 tcgcgaacca gcaccgcggc aacgccatca cgcagaaggc ggtgcgcctc gcgatcgacg
      541 aaggccacct caggcgcgac accgacccgg agcagatcgc cttcgagatg ctcggcatcg
      601 tgctcgcgag ccacaaccac cggcggctgc tcggcgacaa ggagacgcgc aagcgcgcgc
      661 tcgtcgcctt cgacgagctc atcgcgcgtc acgccgccgc gcgcaagccg ctcaaagccg
      721 tttcccgata ggaaaggaga tcccatgccg cattccgctg cgctccccag aattagcacg
      781 aacgttcgat tcgcgactgc gcgcttcctc gctcccgatc tcgccggcgc gtgggccgag
      841 cgcctgttcc ttacgccgcc caagcggagc gacggcgtcg ccaccgcatt ggatttgatc
      901 gatggaaaag caagtttcat cgagcacaag ggccgacaga tcgccatctg gcgctggggt
      961 tcgcgcgacg cacccgcggt gctgctcgcc catggctggg gcggccatgc cgcgcagatg
     1021 cgcggcttcg tcttcccgct gctcgccgcg ggctaccgcg tcatcgccta tgaccagccg
     1081 gcgcacggca tctcgggagg ccggctcacg gggctgccgg atttcgccga ggtcatgtcc
     1141 gaggtcgcct ggcaccacgg ggaagtacgc gcggtcatcg cgcactcgct cggtggcgca
     1201 gcggccgcgc tcgcgctcgc ccggggactg ccgctcgaga aggcggtgct ggtcagccca
     1261 cccgcggacc tcgtcggcta ctctcgccgc ttcgcgcgct ggtactggat tccggagcgc
     1321 gcgcgccgct cgatgcaggc cgcgatcgag gagcgctacg gcgtgcgctg ggacgagctc
     1381 gagctctcgc gcctcgcgcc gcggctctcg gccccggcgc tcgtgatcca cgatcgcgac
     1441 gatcgcatgg tgtcgtggac ccagggcgcg agcttcgcgc gattgtggcc gggcgcgcgc
     1501 ctgctgagca ccgacggact cggccatcgg cgcatcctgc aggatgaaaa ggtgacgcag
     1561 gcggctgccg atttcatctc cgggcgctct tcgatcgcga gccccgcggt tccggcgctg
     1621 ccgcagccgg cgccgcttta ttgaagggaa aaatatgaaa atggagaagc gattgcgcag
     1681 tcttgcgatc acctggagcg atcctcagga gctcgcggca gcgggccgca cgatggcggg
     1741 aatcgacttc ctgcgcgcga tcctcgacgg ccggctgccg ctgccgccga tctgcgagct
     1801 gctggggttc cgcccggtag aggtcgaacc gggccgcgtg gtgttcgagg tgcttccggg
     1861 cgaacaacat tacaatccaa tcggcgtggt gcacggcggg ctcgcgatga cgttgctcga
     1921 ctccgcgatg ggctgcagcg tgcagtctcg gatgcccgcg ggtggcggct acacgaccct
     1981 cgaagccaag acgaacctgg tccgagccat cagttcacag acggggaaac tgcgcgcggt
     2041 aggcaagctg atcaatctgg gcaaacgcgt ggcgaccgcg gaaggtcgcc tggaagatct
     2101 ttccggcaag ctgtacgcgc acgcctcgac cacctgcctg gtgcttgcgc cttagcgttt
     2161 cctgattttc agattgcgcc acgcctcgag cgcggcgcgg tgcttttcca aggcttccgc
     2221 ataggtatcg aagacgcaag gcgtgcagcc cttaccgcaa cactcctctg gcgacggctc
     2281 ccggggcggc agcgccggca gtgcttcttt tcctcctcgc ttcacgccgg agcgagaacc
     2341 ggtaccgccc cgccggcctg actccagcta aggcgccggg ggtggaactc ggcaaggctc
     2401 tcgcggtggc cgatcgaaac cagtgtggta tcgggcaatc gctctttgag cagccggtat
     2461 agcgcctgct gcgcgtcatc gggcagcgac gccgtcgcct cgtcgaggaa cagccagtcg
     2521 gggcggtaga gcagcgcgcg ggcgaatgcg agccgctgct gctcccctcc ggaaagcacc
     2581 tgtgcccagt tctcgctgcg ctcgagctcg tcggcgagct gcgccagccc taccgcgcgc
     2641 agcgcttctt tcaccgcctc gtcggaatgc atcgcagtct cagcgggata gcacactgca
     2701 tgtttcacgg agccgatggg gaggtacggc ttttgcggca ggaacagcag acgtgcgccc
     2761 ttcggcagcc ggatgcgccc cttccagtac ggccatatcc cggcaagcgc gcgaaacagc
     2821 gtcgacttgc cggcgccgga gggacctgaa accagaacgt cctcccgggg cttgagctcc
     2881 agggtcgccg cggcgagaag cggcctgccc tgcggcagat tgagctgcag gtcttcgagc
     2941 gcgacggcat tgcccgcctg ttccacgcgc tcgccctcga ttttcagctc gcgcacgcgc
     3001 tcgagcgacg ccgagaatcc gatcagccgg tcgaccgtcg ccttccaggt ggcgaactgc
     3061 gtgtaggcgc tgatgaacca cgacaacgcg ccctgcacct ggccgaaggc cgacgcggtc
     3121 tgaaatattc cccccatctg catggcgcct gaaaagaacc tgggcgacac cacgacatac
     3181 gggaagatga tggcgagctg gccatagaag gaggtgaacc accccagctg cttctgcttg
     3241 cgcatcagcg cccaccagtt gccgatgact gccgcgaacc ggtcgcggaa gcgaaccagc
     3301 tcctcgcctt cgccgtgata cagggcgacg ccttcgctgt tctcgcgcag gcgaaccagc
     3361 gaaaagcgaa agtccgcctc gaagcgctgc tggttgaagt ccaggccgat gag
//
LOCUS       DASPXW010000300         3008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_968256, whole genome shotgun sequence.
ACCESSION   DASPXW010000300 DASPXW010000000
VERSION     DASPXW010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968256"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VEL04_06830"
     CDS             complement(<1..194)
                     /locus_tag="VEL04_06830"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656598.1"
                     /note="catalyzes the formation of 2-C-methyl-D-erythritol
                     4-phosphate from 1-deoxy-D-xylulose-5-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HYT48118.1"
                     /translation="MNIAVLGATGSIGASTLDVIGRHPGRYRVFALTAHASAEPLLAL
                     CRAHRPSYAVLSGVPEDRAL"
     gene            complement(200..973)
                     /locus_tag="VEL04_06835"
     CDS             complement(200..973)
                     /locus_tag="VEL04_06835"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HYT48119.1"
                     /translation="MSSTLATRVATATALITALLAALFFLPRPWLAVMMVGVVAAGAY
                     EWGRLCRVNPAAYAPVVAASFAAAAWAGFAQPVFIAASAFWLVAVPLWLWRGVEARHG
                     ALLAAAGLAVLVPAALAVMAFSPVQVLAVLGIAWAADISAYFVGRRFGRHKLAPAVSP
                     SKTWEGAAGGLAGVLAYAIICGLFLSGIAWLPYLAAAALLAVVSIVGDLLESAAKRQA
                     SVKDSGALLPGHGGILDRIDSATSTLPLAAALAPWIIGQ"
     gene            complement(970..1731)
                     /gene="uppS"
                     /locus_tag="VEL04_06840"
     CDS             complement(970..1731)
                     /gene="uppS"
                     /locus_tag="VEL04_06840"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HYT48120.1"
                     /translation="MTEASSTQTIPAPGEMPRHVAIIMDGNGRWAKRQRLPRIAGHRR
                     GVEAVRAMVGACAERGIQYLTLFAFSSENWRRPPEEVALLMQLFQAALSNEVEKLHRN
                     GVRLRVVGDTRRFAPKLRGLIEQAESLTAGNQRLTLSIAANYGGRWDILQALSKLQSE
                     RGDAPIDESSLSPYLAMSYAPEPDLFVRTGGEQRISNFLLWQLAYTELYFTDTLWPDF
                     DAAALDAAIASYRARERRFGRTSDQIEAAKRAEVA"
     gene            complement(1728..2288)
                     /gene="frr"
                     /locus_tag="VEL04_06845"
     CDS             complement(1728..2288)
                     /gene="frr"
                     /locus_tag="VEL04_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HYT48121.1"
                     /translation="MVIAELKKTTEQKMDKSVQAFKTDLAKVRTGRAHTGLLDHISVD
                     YYGTPTPLNQVAKVTLLDARTIGVTPFEKKLTQAIEKAIRDSDLGLNPAAAGETVRVP
                     MPPLTEERRKELIKVVRHEAENARVAVRNLRRDAIHHLKEALKKKEVSENDERRAQDE
                     VQKMTDRHTGDIDKLLQQKEAELMAV"
     gene            complement(2314..2970)
                     /gene="pyrH"
                     /locus_tag="VEL04_06850"
     CDS             complement(2314..2970)
                     /gene="pyrH"
                     /locus_tag="VEL04_06850"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HYT48122.1"
                     /translation="MESIVAEIAAVARQGVEIGEVIRLGVQVAIVIGGGNIFRGVAPG
                     AAGMDRATADYMGMLATVMNALALQDAMRRAGLASRVQSALNIEQVVEPYIRGKAIRY
                     LEEGKVVIFAAGTGNPFFTTDTAAALRGSEMAAEIVIKATKVDGVYTADPRRDPRAQR
                     YARISFDEAIARNLRVMDATALTLCRDQKLPINVFNIFKKDALRRVILGEDEGTLVHV
                     "
BASE COUNT          476 a         1028 c         1019 g          485 t
ORIGIN      
        1 ctcagcgcac ggtcttcggg gacgcccgac agcaccgcgt acgacggacg atgcgcgcgg
       61 cacaaggcaa gcaaaggttc tgcggacgca tgcgcggtga gcgcgaacac gcgatagcgg
      121 ccggggtggc ggccgatgac gtcgagcgtg ctcgccccga tcgagccggt ggcgccgagc
      181 actgctatgt tcatcgcctt cattgtccaa tgatccaggg cgcaagcgcc gcggcaagcg
      241 gcagggtcga ggtcgcgctg tcgatccggt ccagtatccc cccgtggccc ggcaggagcg
      301 cgccgctgtc cttgactgag gcctgcctct tggctgcaga ttccaacaga tctcccacga
      361 tgctgaccac ggcaagcagg gccgccgccg cgaggtacgg cagccaggca attcccgaca
      421 ggaaaagccc gcagatgata gcgtaggcaa gcacgccggc aagtccgcca gcagcgcctt
      481 cccaggtctt ggacgggctg actgccggcg cgagcttgtg cctgccgaat cgcctgccga
      541 cgaagtatgc cgagatatcg gcggcccaag cgatgccgag cacggcgagg acttgcaccg
      601 gcgagaaagc catgaccgcg agcgccgccg gaaccagcac cgcgagcccg gctgccgcca
      661 gcagagcgcc gtgccgcgcc tcgacgccgc gccacagcca cagcggcacg gccaccagcc
      721 agaacgcgct cgcggcgatg aaaaccggct gcgcgaagcc ggcccaggcc gcagcggcga
      781 aactcgcggc aacgacaggc gcatacgctg ccgggttgac gcggcaaagc cgtccccatt
      841 cgtaggcgcc ggcggcgacg accccgacca tcatgacggc aagccagggc ctgggcagaa
      901 aaaacagcgc tgcaagcaga gcggtgatca gcgccgtcgc ggtggcgacg cgagttgcga
      961 gcgtgctgct caggcgacct cggcccgctt cgccgcttcg atctggtcgc tggtgcggcc
     1021 gaagcgccgc tcgcgggcgc gataggaggc gatcgccgcg tcgagcgccg cggcgtcgaa
     1081 gtccggccac agcgtgtcgg tgaagtaaag ctcggtgtag gcgagctgcc agagcaggaa
     1141 attactgatg cgctgctcgc cgccggtgcg gacgaacaga tccggctcgg gcgcgtagct
     1201 catcgccagg taaggagaca aagaagactc atcaatcggc gcgtcgccac gctcggactg
     1261 caattttgaa agcgcttgca ggatgtccca gcgcccgccg tagttggccg caatgctgag
     1321 cgtaaggcgc tggttgcccg ccgtcaggct ctcggcctgc tcgatcaggc cgcggagctt
     1381 gggggcgaag cgccgcgtat cgccgactac ccggagccgc acgccgttgc gatgcagctt
     1441 ctccacttcg ttcgaaagcg ccgcctggaa gagctgcatc agcagcgcca cctcctcggg
     1501 cggccggcgc cagttctcgg agctgaaggc aaacagcgtc aggtactgga tgccgcgctc
     1561 ggcgcaggcg cccaccatgg cgcgcaccgc ctcgacgccg cggcggtggc cggcgatgcg
     1621 cggcaggcgc tggcgcttgg cccagcggcc gttgccgtcc atgatgatcg cgacatggcg
     1681 cggcatctcg ccgggggccg gaatggtctg ggtcgagctc gcttcggtca tacggccatc
     1741 agctcggctt ccttctgctg cagcagcttg tcgatgtcgc cggtatgccg gtcggtcatc
     1801 ttctgcacct cgtcttgcgc gcggcgctcg tcgttctccg agacctcctt cttcttgagc
     1861 gcttccttga gatgatggat ggcgtcgcgc cgcaggttgc gtacggcgac gcgcgcgttc
     1921 tcggcctcgt gccgcaccac cttgatcagc tccttgcgcc gctcctcggt gagcggcggc
     1981 atcggcacgc gcaccgtctc gcccgcggcc gcggggttga ggccgaggtc cgagtcgcgg
     2041 atcgccttct cgatggcctg cgtcagcttc ttctcgaagg gggtgacgcc gatggtgcgc
     2101 gcgtcgagca gcgttacctt ggcgacctgg ttgagcggag tcggcgtgcc gtagtagtcg
     2161 acgctgatgt ggtcgagcag gcccgtgtgc gcgcggccgg ttcgcacttt ggcgaggtcg
     2221 gtcttgaacg cctgcacgct cttgtccatc ttctgctcgg tggtcttctt cagctccgcg
     2281 atcaccatcg ccgcctcccg caatgctgca gaatcatacg tgaaccaggg tgccctcgtc
     2341 ctcgccgaga atcacgcggc gcagcgcgtc cttcttgaag atgttgaaga cgttgatcgg
     2401 cagcttctgg tcgcggcaca aggtcagcgc ggtggcatcc atgacccgga gattgcgcgc
     2461 gattgcctcg tcgaagctga tgcgcgcata gcgctgcgcg cgcggatcgc gccgcgggtc
     2521 ggcggtgtat acgccgtcca ccttggtcgc cttgatgacg atctcggccg ccatctcgct
     2581 gccgcgcagc gccgccgccg tgtcggtggt gaagaaggga ttgccggtgc cggcggcgaa
     2641 gatcaccacc ttcccctcct ccaggtagcg gatcgccttg ccccggatgt aaggctcgac
     2701 cacctgctcg atgttgagcg ccgactgcac gcggctcgcg agccccgcgc gccgcatcgc
     2761 atcctgaagc gcgagggcgt tcatgacggt ggcgagcatg cccatgtaat cggccgtggc
     2821 acgatccatt cccgcagcgc cgggcgcgac gccacggaag atgttgcccc cgccgatcac
     2881 gatggccacc tgcacgccga ggcgaatcac ctcgccgatc tcgacgccct gcctggccac
     2941 cgcggcgatc tcggcgacga tcgattcgat ggtcggccgg caacatcttc cgcggcgtgg
     3001 cgcccggc
//
LOCUS       DASPXW010000301         2471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_339419, whole genome shotgun sequence.
ACCESSION   DASPXW010000301 DASPXW010000000
VERSION     DASPXW010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339419"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2463)
                     /locus_tag="VEL04_06855"
     CDS             complement(<1..2463)
                     /locus_tag="VEL04_06855"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HYT48123.1"
                     /translation="MPLRLFPVYRDLAQLKGTPAVSEKDLFFPDLSVKAPPHRKPKTL
                     APEAIAGHVRKQRALFQRGLLAWLRKPPAGLEDMRRAVNQLHKVAAQMPEPQAIWWVA
                     GGLLDAIEHTKDAGWLVGAKALGNKIDRQMREGSKTVNELLLRELLYAIAKCKPATPR
                     LKEIRQLYQLDSLFPRPDAAAGVLEFDIERLEVALYDLHSLLEALKRAWVQYVSGEHK
                     TAGRFRELVASFKVKAKDLGNQHFVKLLDAIALVAAKLPDPYPQQAEVLVIEMASAFL
                     LVEHVVDNFTNPAPDLEQQIVIMGGWLLDAAMGKSAGEPPPGLRPDLSERIGALHLRS
                     QVAKEILTNLQHVERVLDAFARDTTKRATLPDLQPYLRQMHGALVVLGLTRAAEALAI
                     CERMIADCAKDNHAALAEDMDWIAEGLSSLGFFLEPCRHGREPAAEAIALFFRRYEKR
                     NAPPSLDATQRLKSPLTAAAPEAPQPETPVPLAPQPVAARPAVDQDLLAVFLEEAGEV
                     LQTIGKTLPECRARPADREALTTVRRSFHTLKGSGRMVGLMDLGEAAWEVEQVMNRWL
                     EQKRPATPALLELVGAASASFASWIARLREGGLGGEVDARHIVELAARLKAEAPPPDE
                     VTVGPVRLARGFFEIYVKEAAQHIATLEAQCGEWHKMPGADASHEFMRAAHTLASSSR
                     TAGFGAVADLAAVLEHWTAFARQTTAAADVEAVQDTIANLRAMVESLGRREPPAQGEE
                     ATRRLQALTARLEAHPPPPVEAKATPAAAPAAQPPQAVVREKRVIRDDIDPQLLLVFL
                     EEAQELVPHIGSDLRDWKANPSD"
BASE COUNT          355 a          848 c          857 g          411 t
ORIGIN      
        1 gtccgatgga ttcgccttcc agtcgcgcag atcgctgccg atgtgcggca cgagctcctg
       61 cgcctcctcg agaaacacca gcaggagctg cgggtcgatg tcgtcgcgaa tgacgcgctt
      121 ctcgcgcaca accgcctgcg gaggctgagc cgcgggggct gcggccggag ttgctttcgc
      181 ctcgaccggc ggcggcggat gcgcttccag gcgggcggtg agcgcctgca ggcgccgcgt
      241 ggcctcctcg ccttgcgccg gcggctcgcg ccggcccagg ctctcgacca tggcacggag
      301 gttcgcgata gtgtcttgga cggcttccac gtccgccgca gcggtggtct ggcgggcgaa
      361 cgcggtccag tgctcgagca cggcagcgag atcggcgacg gcgccgaatc cggcggtgcg
      421 cgagctgctt gccagagtgt gcgcggcgcg catgaattcg tgcgaggcgt cggcgccggg
      481 catcttgtgc cattcgccgc attgcgcctc cagcgtggcg atgtgctgcg ccgcctcctt
      541 gacgtagatt tcgaagaaac cgcgcgcgag cctgaccggg cctacggtca cctcgtcggg
      601 cggcggcgcc tctgccttga gccgtgccgc aagctcgacg atatgcctcg cgtccacctc
      661 cccgcccagc ccgccttcgc ggagccgcgc gatccagctg gcgaaggacg cgctcgccgc
      721 gccaaccaac tcgagcagcg ccggcgttgc cggccgcttc tgctcgagcc agcggttcat
      781 gacctgctcg acttcccagg ccgcctcgcc gagatccatc aatccgacca tccgcccgct
      841 gcccttcaag gtgtggaaac tccggcgcac cgtggtgagc gcctcgcggt ctgccgggcg
      901 cgcgcggcat tcgggcagcg ttttacctat ggtctgaagc acctcgcccg cctcttctag
      961 gaacacggcg agcagatcct gatcgaccgc agggcgcgcc gcaacgggtt gcggcgcgag
     1021 cggcaccggc gtctcgggct gcggcgcctc gggcgctgcg gccgtcagcg gcgacttcag
     1081 gcgctgggtt gcatccagcg acggaggcgc gttgcgcttc tcgtagcggc ggaagaagag
     1141 cgcgatcgcc tcggcggcgg gttcgcgccc gtggcggcag ggctcgagga agaatcccag
     1201 gctgctcaag ccttcggcga tccagtccat gtcctcggcc aaagccgcat gattgtcctt
     1261 ggcgcagtcg gcgatcatgc gctcgcagat ggcgagcgct tccgcggcgc gcgtcaggcc
     1321 gaggacgacc agggctccgt gcatctgcct gagatacggc tgcaggtcgg gcagcgtcgc
     1381 gcgctttgtc gtatcgcgcg cgaaggcgtc gagcacccgc tccacgtgtt gcagattggt
     1441 caggatctcc ttggccacct gcgaacgcag gtgcagggcg ccgatgcgct cgctcaggtc
     1501 gggccgcagg cctgggggcg gctcgccagc ggacttgccc atcgcggcat cgagcagcca
     1561 gccacccatg atgacgattt gctgctccag atccggcgcc ggattggtga aattgtcgac
     1621 gacatgctcg acgagcagga acgccgacgc catctcgatc accaggacct cggcctgctg
     1681 cgggtaggga tcaggcagct tggcggcgac cagcgcgatc gcgtcgagca gcttgacgaa
     1741 gtgctggttg ccgagatcct tggccttgac tttgaaggag gcgaccagct cccggaaccg
     1801 gccggcggtc ttgtgctcgc ccgagacgta ctgcacccag gcgcgcttga gcgcctcgag
     1861 cagcgagtga aggtcgtaca acgccacttc gagccgctcg atgtcgaact cgaggacgcc
     1921 tgccgcggcg tcaggccgcg gaaagaggct gtcgagctgg tacagctgcc tgatttcctt
     1981 caaccgcggc gtcgccggct tgcacttcgc gatcgcgtaa agcagctcgc gcaaaagcag
     2041 ctcgttgacg gttttcgatc cctcgcgcat ctggcggtcg atcttgttgc cgagggcttt
     2101 ggcgccgacc agccatccgg cgtccttcgt gtgctcgatg gcatcgagca ggccgcccgc
     2161 cacccaccag atcgcctgcg gctcgggcat ctgcgccgcg accttgtgca gctggttgac
     2221 ggcccggcgc atgtcctcga gcccggccgg cggcttgcgc agccaggcga gcagcccgcg
     2281 ctggaagagc gcgcgctgct tccgcacatg gccggcgatc gcctcggggg ccagcgtctt
     2341 cggcttgcgg tggggcggcg ccttgacgga gaggtccgga aagaacaggt ccttctccga
     2401 gaccgccggc gtgcccttga gctgcgccag gtcgcgatac accgggaaaa ggcgcagcgg
     2461 cacgtccgct t
//
LOCUS       DASPXW010000302         3274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_968668, whole genome shotgun sequence.
ACCESSION   DASPXW010000302 DASPXW010000000
VERSION     DASPXW010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968668"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1133)
                     /locus_tag="VEL04_06860"
     CDS             complement(<1..1133)
                     /locus_tag="VEL04_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HYT48124.1"
                     /translation="MELSKSSFWGYRRENGRVGVRNHVIIIPVDDLSNAAAQAVENNI
                     KGTMALAHPYGRLQFGADLDLHFRTLIGAGSNPNVAAAVVIGIEDGWAKRVADGIAQT
                     GKPVSFFGIEGHGDLETVKRASQAAKDYLQHASELRREERPLKDLWVSTKCGESDTTS
                     GIGSNPCVGNAFDKLYPQGVTLVFGETTELTGGEHLVAARCRNDEVRKKFQFVFDRYQ
                     TVIDRHKTSDLMDSQPTKGNIAGGLTTIEEKALGNIQKIGRKCVVDGVLDKAETPTGP
                     GLWFMDSSSAAAEMVTLCAAAGFVVHFFPTGQCNVIGNPILPVIKLCANPRTVRLMAD
                     HVDVDVSGITRRELTMDQAGDKLLECMFRTANGRLTAAEALGHR"
     gene            complement(1138..1419)
                     /locus_tag="VEL04_06865"
     CDS             complement(1138..1419)
                     /locus_tag="VEL04_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008159569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HYT48125.1"
                     /translation="MIHFVVHEEGDGVGVVVVEGVKAGQPLSGWIMEDDKDIEVVAKN
                     DIPIGHKVALKDYRPGDSVIKYGVDIGKVVQPIRRGEHLHVHNVKTKRW"
     gene            1440..2948
                     /locus_tag="VEL04_06870"
     CDS             1440..2948
                     /locus_tag="VEL04_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HYT48126.1"
                     /translation="MHDRRRFLKSLGALGAATVLPRITSYPFTLGVASGYPAPGGISL
                     WTRLMNIPDPVPVPVRWEVAADDAMRQVIASGEQAADPAWAHALHVDVQGLEPERWYW
                     YRFSAGDAQSPVGRTRTAPLAASAPARLRFAFASCQQYEQGYFGAYRHIIAGEPDLVF
                     FLGDYIYESSWGKELVRSHGAPEPYTLDEYRARYALYKSDPDLQAAHAACPWVVTWDD
                     HEVDNDYADDRPEDGMGREEFLRRRAAAYRAYFEHMPLPQSMRPDGPRMRIYTHLDWG
                     TLARFCVLDDRQYRSWHACPRPGRRGGSNTVDVSECTRIASPGRSILGRAQERWLDGR
                     LAISRARWNVLAQQTRMAQMDELPGPGRRAWTDSWDGYPAARQRLLDSIAARKIQNPV
                     VIGGDIHAFNVCQLKADFDDARSAVVASEFVGTSITSQGWPQERINEYLPDNPHVLYA
                     DSRYRGYVGVELTPKRWLAELRAMQTVRQRDSACSTLASFVVEDGEPGPLRA"
     gene            complement(2945..>3274)
                     /locus_tag="VEL04_06875"
     CDS             complement(2945..>3274)
                     /locus_tag="VEL04_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48127.1"
                     /translation="WPLAVLVMLAGCSHTEVRVDSGSAAARTQGALQVRVEGGRGLAT
                     LLGLTFLTAAMFDYERDRMRYRENPFMPLGEARPDAAPPLAAERTISEQDCTRPLEDF
                     SANLKCR"
BASE COUNT          519 a         1110 c         1064 g          581 t
ORIGIN      
        1 cgatggccga gcgcttcggc ggcggtgagg cggccgttgg cggtgcggaa catgcactcg
       61 agcagcttgt cgcccgcctg gtccatggtg agctcgcgcc gcgtgatgcc ggagacgtcg
      121 acgtcgacgt ggtccgccat caggcgcacg gtgcgcgggt tggcgcacag cttgatcacc
      181 ggcaaaattg ggttgccgat gacgttgcat tgcccggtgg ggaagaaatg caccacaaag
      241 cccgcggcgg cgcacagcgt caccatctcg gcggcggccg aggacgagtc catgaaccat
      301 aggccggggc cggtcggcgt ctcggccttg tcgagcacgc cgtcgaccac gcacttgcgg
      361 ccgatcttct ggatgttgcc gagcgccttt tcctcgatag tggtgaggcc gccggcgatg
      421 ttgcccttgg tcggctgcga gtccatgagg tcgctcgtct tgtgccggtc gataaccgtc
      481 tgatagcggt cgaagacgaa ctggaatttc ttgcgcacct cgtcattgcg gcagcgcgcg
      541 gcgaccaggt gctcgccgcc cgtcaactcc gtagtttccc cgaagaccag ggtgacgccc
      601 tgcgggtaga gcttgtcgaa cgcgttgcca acgcacgggt tcgagccgat gccggaggtg
      661 gtgtcggatt cgccacattt ggtcgagacc cacaggtcct tgagcggccg ctcctcgcgc
      721 ctgagctccg aggcatgctg gaggtaatct ttcgccgcct gcgaggcgcg tttcaccgtc
      781 tccagatcgc catggccttc gatgccgaag aaggaaaccg gcttgccggt ctgcgcgatg
      841 ccgtccgcga cgcgcttcgc ccagccgtcc tcgatgccga tcacgaccgc cgcagcgacg
      901 ttcgggttcg agccggcacc gatgagcgtg cggaaatgca ggtccaggtc ggcgccgaac
      961 tgcagccggc cgtagggatg cgcgagcgcc atcgtgccct tgatgttgtt ctccaccgcc
     1021 tgcgctgcag cattcgacag gtcgtcgacc ggaatgatga tgacgtggtt gcgcacgccg
     1081 acgcgcccgt tctcgcggcg gtagccccag aagcttgatt tcgagagttc catggtctta
     1141 ccagcgcttg gttttcacgt tgtggacatg caggtgctcg ccacgcctga tcggctgcac
     1201 cacttttccg atgtcgacgc cgtacttgat cacgctgtcg ccggggcggt agtccttgag
     1261 cgcgaccttg tggccgatcg gaatgtcgtt cttcgccacc acctcgatat ccttgtcgtc
     1321 ttccatgatc cagccgctga gcggctgtcc ggccttgacg ccttccacca cgaccacgcc
     1381 gacgccgtcg ccttcctcgt gcaccacgaa atgaatcata ggagcgctat tctattcgga
     1441 tgcatgaccg tcgccgcttc ctgaaatcgc tcggcgcgct tggcgcggcg acggttttgc
     1501 cacgcatcac ctcgtacccg ttcacgctcg gcgtggcctc gggctatccg gcgcccggcg
     1561 gcatctcctt atggacgcgg ctgatgaaca ttcccgatcc ggtgcccgtc ccggtgcgct
     1621 gggaggtcgc tgccgacgat gcgatgcgcc aggtcatcgc ctccggcgag caggctgccg
     1681 atcccgcatg ggcgcacgcg ctgcatgtcg acgttcaggg actcgagccc gagcgctggt
     1741 actggtaccg cttcagcgcg ggcgatgcgc aaagccccgt cgggcgcacg cgtaccgcgc
     1801 cgctcgccgc ctcggccccc gcgcggctgc gcttcgcttt cgcttcctgc cagcaatacg
     1861 agcagggcta tttcggcgcc taccggcaca tcatcgccgg cgagcctgac ctcgtgttct
     1921 ttctcggcga ttacatctac gagtcgagct ggggcaagga gctggtgcgc tctcacggcg
     1981 cgcccgagcc ttacacgctc gatgagtacc gtgcgcgtta cgcgctctac aagagcgacc
     2041 cggacctgca ggccgcgcat gccgcctgcc catgggtcgt cacctgggac gaccatgagg
     2101 tcgacaacga ttacgccgac gaccggcccg aggacggcat ggggcgcgaa gagttcctgc
     2161 gccggcgggc ggccgcctac cgcgcctact tcgagcacat gccgctgccg cagtcgatgc
     2221 gccccgacgg cccgcggatg cgcatctaca cccacctcga ctggggaaca ctggcgcgct
     2281 tctgcgtgct cgacgaccgg caataccgct cctggcacgc ttgcccgcgt cccgggcggc
     2341 gcggcggctc gaacacggtc gacgtatccg agtgcacgcg catcgcgagc cccgggcgct
     2401 cgattctcgg ccgcgcgcag gaacgctggc tcgacggcag gctcgccatc tcgcgcgcgc
     2461 gctggaacgt gctcgcgcag caaacgagga tggcgcagat ggacgagctg cccggccccg
     2521 gacggcgcgc ctggaccgac agctgggacg gctatcccgc ggcgaggcag cgcctgctcg
     2581 attcgatcgc ggcaagaaag atacaaaacc cggtagtgat cggcggcgac atccatgcct
     2641 tcaacgtctg ccagctcaag gccgatttcg acgatgcgcg ctccgcggtg gtagcgagcg
     2701 aattcgtcgg cacctccatc acctcgcagg gctggccgca ggagcgcatc aacgaatatc
     2761 ttcccgacaa cccgcatgtg ctgtacgccg acagccgtta ccgggggtat gtgggcgtcg
     2821 agctgacgcc gaagcgctgg cttgccgaac tgcgtgcgat gcaaaccgtc cggcagcgcg
     2881 actcggcctg ctcgacgctg gcgtctttcg tggtggaaga cggagaaccg ggaccgctaa
     2941 gagcttaacg gcacttcaaa ttggcagaga agtcctcgag cggtcgcgtg caatcctgct
     3001 cgctgatcgt acgctcggcg gcgagcggcg gcgccgcgtc gggtcttgct tcaccgagtg
     3061 gcatgaaagg attttcgcga tagcgcatgc ggtcccgctc gtaatcgaac atcgccgccg
     3121 tcaggaaggt cagcccgagc agcgtagcga gtccgcgccc gccttcaacc cgcacctgca
     3181 aagcgccctg cgtgcgggca gcagcgctgc ccgagtcgac tcgcacctcg gtgtgcgagc
     3241 acccggcgag catcacgagc accgcgagcg gcca
//
LOCUS       DASPXW010000303         2022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_466440, whole genome shotgun sequence.
ACCESSION   DASPXW010000303 DASPXW010000000
VERSION     DASPXW010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466440"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="VEL04_06880"
     CDS             <1..1011
                     /locus_tag="VEL04_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48128.1"
                     /translation="LQRRLLDEINVSIKEIHEKVLAAYRDAYEERHKILNSFMPFWRS
                     LDKTLAQADQKMTNLNDRAAQIDAQMQKYEQIVAKTDKAEHALTISAVTQFAIATLVL
                     AIAAGGALINFKLIALPMSEMVGAGDYLTGSLRTSEVAALVIIFVEATMGLFLMETLR
                     ITHLFPRVHNLSDQMRRRMMWVAFTLLVTLAGIEAALALMRDMLIADKQALLQSLAAA
                     QPMISDSLLTRIPTAGQMLLGFILPFALAFVAIPLESFMVSARTVGGAALVLLARGTA
                     FTLRLAGNLIRNLARVLITFYDVVIVLPLLVERMAKASHPKQIAEPPVVEEKKLAARR
                     GS"
     gene            1008..1670
                     /locus_tag="VEL04_06885"
     CDS             1008..1670
                     /locus_tag="VEL04_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYT48129.1"
                     /translation="MNKRSFLASLPALGLAAWLPGCTDQRSHAQAVYMLVDTSGTYAE
                     EVGKARVIVNYLLGTLQPGDSLAVARVRSRSFSERDIIAKATFDNRPSQANAQKRAFS
                     EQVEAFTRASRSSAYTDITGGLIQGSLFLNETGAGRKTILIFSDMQEDLDKTTVRDFA
                     IDLKNIRVVAVNVVKLKTDNLDPRRYTGRLAEWQKRVEGAGASEWRVINDIEHLDRLL
                     KS"
     gene            complement(1704..>2022)
                     /locus_tag="VEL04_06890"
     CDS             complement(1704..>2022)
                     /locus_tag="VEL04_06890"
                     /inference="COORDINATES: protein motif:HMM:NF023125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3293 domain-containing protein"
                     /protein_id="HYT48130.1"
                     /translation="ALLEAEGAATAAYITAANPLGERRSKARNALGVGALEDLIAASG
                     YPRLAGEGRDPEGRWPAEPSLLVIGIYREHAAALGRLFGQNAIVFVEKGAAPELVLLA
                     PAP"
BASE COUNT          338 a          708 c          675 g          301 t
ORIGIN      
        1 ctgcagcggc ggctgctcga cgagatcaac gtctcgatca aggagatcca cgagaaggtg
       61 ctggcggcgt accgcgacgc ctacgaagag cggcacaaga tcctcaacag cttcatgccg
      121 ttctggcgct cgctcgacaa gacgctggcg caggccgacc aaaagatgac caacctgaac
      181 gaccgcgcgg cgcagatcga cgcccagatg cagaagtacg aacagatcgt cgccaagacc
      241 gacaaggccg agcatgcgct gacgatctcg gcggtcacgc agttcgccat cgccacgctg
      301 gtgctcgcca tcgccgcggg cggcgcgctc atcaacttca agctgatcgc gctgccgatg
      361 tcggagatgg tcggcgccgg cgactacctg accgggtcgc tgcgcacctc ggaagtcgcc
      421 gccctggtga tcatcttcgt cgaggcgacg atgggcctgt tcctgatgga gacgctgcgc
      481 atcacgcacc tcttcccgcg cgtccacaac ctgagcgacc agatgcgccg ccgcatgatg
      541 tgggtggcgt tcaccctgct cgtgaccctg gcggggatcg aggcggcgct cgcgctgatg
      601 cgcgacatgc tgatcgccga caagcaggcg ctgctgcagt cgctcgccgc cgcccagccg
      661 atgatcagcg acagcctgct gacgcggatc ccgaccgccg gccagatgct gctcggcttc
      721 atcctgccgt tcgcgctggc gttcgtggcg atcccgttag agtcgttcat ggtgtctgcc
      781 cgcaccgtgg gcggcgccgc gctggtgctg ctcgcgcgcg gcacggcctt cacgctgcgc
      841 ctcgccggca acctgatccg caacctcgcc cgggtgctga tcacgttcta cgacgtggtc
      901 atcgtgctgc cgctgctcgt cgagcgcatg gcgaaggctt cccacccgaa gcagattgcc
      961 gagccgcccg tggtggagga gaagaagctc gccgcaagga ggggctcatg aacaagcgct
     1021 ccttcctcgc ctcgctgccg gccctcgggc tggcggcctg gctgccaggc tgcaccgacc
     1081 agcgcagcca tgcgcaggcg gtgtacatgc tggtcgacac ctcgggcacc tatgccgagg
     1141 aggtcggcaa ggcccgcgtc atcgtcaact acctgctcgg cacgctgcag cccggcgact
     1201 cgctggcggt ggcgcgcgtg cgcagccgca gcttcagcga gagggacatc atcgccaagg
     1261 cgaccttcga caaccgcccc tcgcaggcca acgcccagaa gcgcgccttc agcgagcagg
     1321 tcgaggcctt cacgcgggca tcgcgcagca gcgcctacac cgacatcacc ggcggcctga
     1381 tccagggctc gctgttcctg aacgagaccg gcgccggcag gaagaccatc ctgatcttct
     1441 ccgacatgca ggaggatctc gacaagacga cggtgcgcga cttcgcgatc gacctgaaga
     1501 acatccgcgt ggtcgcggtc aacgtcgtca agctcaagac cgacaacctc gacccgaggc
     1561 gctacacggg gcggctggcg gagtggcaga agcgcgtcga gggcgcgggc gcctccgagt
     1621 ggcgcgtgat caacgacatc gagcacctgg accggctgct gaaaagctaa ccccccgctc
     1681 gccttcggct cacgaccccc ttgtcagggg gccggggcca gcagcaccag ctcgggcgcg
     1741 gcgcccttct cgacgaacac gatggcgttc tggccgaaca gcctgccgag cgctgcggcg
     1801 tgctcgcggt agatgccgat gacgagcaag ctcggctcgg cgggccagcg cccttccggg
     1861 tcgcgcccct cgcccgcgag gcgcggatag ccggaggcgg cgatgagatc ctcgagcgcg
     1921 ccgacgccga gcgcgttgcg cgccttggaa cggcgctcgc cgagcgggtt ggcggcggtg
     1981 atgtaggccg cggtggcggc gccttcggct tcgagcagcg ca
//
LOCUS       DASPXW010000304         7016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_594577, whole genome shotgun sequence.
ACCESSION   DASPXW010000304 DASPXW010000000
VERSION     DASPXW010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7016
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594577"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..1184)
                     /gene="proB"
                     /locus_tag="VEL04_06895"
     CDS             complement(66..1184)
                     /gene="proB"
                     /locus_tag="VEL04_06895"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HYT48131.1"
                     /translation="MSSPLQKATTLVVKVGSSLVTTAGRGLDVAAIATWAAQIARLRS
                     LGRRMVLVSSGAIAEGMQRLGWAKRPHTMHELQAAAAVGQMGLAQCYESCFREHGLHT
                     AQILLTHADMADRQRYLNARSTLRTLLALGVIPVINENDTVVTDEIKFGDNDTLGALV
                     TNLVEADALVILTDQPGLYESDPRSNVQAKLVETADADDARLEAMAGGTGTALAKGGM
                     LTKVLAARRAARSGAHTVIASGREPEVLVRLAQGELIGTLLTARTAPIAARKQWLADH
                     LTVSGCLRLDAGAVRALVRDGKSLLPIGVVEVTGEFARGEIVGCLDPAGREVARGLVN
                     YSAEETRRIMRRPSGEIESVLGYVDEPELIHRDNLVLL"
     gene            complement(1181..2185)
                     /gene="obgE"
                     /locus_tag="VEL04_06900"
     CDS             complement(1181..2185)
                     /gene="obgE"
                     /locus_tag="VEL04_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HYT48132.1"
                     /translation="MKFIDEAKIEVHAGKGGDGSASFRREKYVPRGGPDGGDGGRGGS
                     VWARADRNVNTLIDYRFARIHRAAHGARGMGSDCNGRSGEDVVLRMPVGTVIRDAESG
                     DWVADLTMDGERALLARGGSGGLGNLHFRSSVNRAPRQFTKGEPGESRSLALELRVLA
                     DVGLLGLPNAGKSTFIRAVSSARPKVADYPFTTLNPSLGVVRVGERSFVVADVPGLIE
                     GAAEGAGLGHQFLRHLARTRILLHLVDVAPFDPGADPACDAKAIALELKKYDAALYRK
                     PRWLVFNKIDLAEDADKRIASILRALRWKRPWFKVSAISGKGCREVCKAIARELAAKK
                     "
     gene            complement(2247..2504)
                     /gene="rpmA"
                     /locus_tag="VEL04_06905"
     CDS             complement(2247..2504)
                     /gene="rpmA"
                     /locus_tag="VEL04_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HYT48133.1"
                     /translation="MAHKKAGGSSRNGRDSQAKRLGVKAYGGERVNAGQILVRQRGTR
                     VHPGVNVGIGRDHTLYARVTGQVQYAVKGEDERNTVSVIPA"
     gene            complement(2507..2821)
                     /gene="rplU"
                     /locus_tag="VEL04_06910"
     CDS             complement(2507..2821)
                     /gene="rplU"
                     /locus_tag="VEL04_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HYT48134.1"
                     /translation="MYAVIKSGGKQYRVESGAKLRVEALSAEVGAAVSFDEVLLVGSG
                     DAVKVGAPLVSGALVKATVVSHGRGDKVRIFKLRRRKHHQKSQGHRQSYTEVRIDDII
                     QG"
     gene            2871..3851
                     /locus_tag="VEL04_06915"
     CDS             2871..3851
                     /locus_tag="VEL04_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012762954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HYT48135.1"
                     /translation="MSFVSMLAPVADDMRRVDELIGRRLDSDVPLVRQVAEYIVASGG
                     KRLRPALVLLACGAAGFREKSGGELRLRLAAVVEFIHTATLLHDDVVDASHLRRGRRT
                     ANAAFGNAAAVLVGDFLYSRAFQMMVEVDDMRVMRVLADATNTIAAGEVMQLMGSHDP
                     EVDEARYLEVIRRKTAKLFEAAARLGGVLARSPGSVEDGLAGYGMHVGTAFQLIDDVL
                     DYSGDEATIGKSLGDDLAEGKPTLPLIRVMRTGTPQQAAMVRRAIVDGGRGEFAGVLQ
                     AMRACGALDYARQAAQREADAASAALAPLAASEFKQSLLELASFSVTRSS"
     gene            3866..3942
                     /locus_tag="VEL04_06920"
     tRNA            3866..3942
                     /locus_tag="VEL04_06920"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3900..3902,aa:Pro,seq:cgg)
     gene            complement(3951..4949)
                     /locus_tag="VEL04_06925"
     CDS             complement(3951..4949)
                     /locus_tag="VEL04_06925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYT48136.1"
                     /translation="MKILVAVPGRLRTVPMSRFVPEAAAALGHQVSVADYSLALAEKL
                     KSRLLRKRSAGDAVAPRLLQAIEEARPQLFLTLYGANVNAQVFAELRKRGVVTANWWL
                     NDPFQFERGAKILPGYDFAFTNARYSVEAYAALGLKNVHFLPTACEPAVHRPLSGRAS
                     LCDISFAGDWSAMREQLVDALLHAGLDVRVFGPWRRKLAKHSAVRARLEHGFFSPQRM
                     AEIFAACKATLNIHTWRGRFDHGLNPRVFEAAACGVPQLVDYKKELDEIFDPAQRAGM
                     LIYRDDGELLRLARKATEDAVELRGRALAASASFQRDHSYRARITELLRVVGGAAA"
     gene            4975..5208
                     /locus_tag="VEL04_06930"
     CDS             4975..5208
                     /locus_tag="VEL04_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HYT48137.1"
                     /translation="MGRLVVIVLLVVVAVWLLRRALRRPGQGPDSAGKPAAPGELVRC
                     AHCGMHLPLAEAHARDDRVYCSEEHARLGPGRA"
     gene            5208..6806
                     /locus_tag="VEL04_06935"
     CDS             5208..6806
                     /locus_tag="VEL04_06935"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYT48138.1"
                     /translation="MDAPLRYPELFTPPRKRSPESFWISLGYFNLYRVAVATLFFAIT
                     MVYGDALNLGSHSLVLFRYVCAAYLLFGIAFQGVLRSLREHFNLQLTLHVSLDIAAIT
                     LLMYASGGVRSGLGVMLLITLTGAAILAPRRFSFLYAALATIALLLEQGYWTLAHDAP
                     TSNFLQPGLVAMGCFAGVGITGWLAQRVATNERLARQRGRELAMQTRVNQLVLQDTHD
                     GVVVLDRDGRVVQFNPQAQRLLRAERLLGGEMADLLPGFDQHWRAWRSGGRGEPSPEV
                     ELRGRVMRLRLLDTGTEEGFAVLFIEDTTRTREQAQQLKLAALGRLTANIAHEIRNPL
                     AAISHAAELLHEEKRAEDRGRLTRIVHDNTQRLERLVSDVLQLSRRDRIAAEPIRVGV
                     WLAGFVDEFVANESVAAGRFVTDVAGDPWIRFDREHLSQVMWNLLRNAMRYARAEPHS
                     VRIALREFADRVELSVIDNGPGVPLSIQGQLFEPFFTTEAKGTGLGLYLARELCATNG
                     ATLEYVGDMPGAHFRIQCREARVA"
     gene            6803..>7016
                     /locus_tag="VEL04_06940"
     CDS             6803..>7016
                     /locus_tag="VEL04_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYT48139.1"
                     /translation="MKRAERAAAPRGAQVLVVDDEPDIRELLELTLVKMGLGVQAVGS
                     IAEAKECLKSARYDLCLTDMRLADGEG"
BASE COUNT         1061 a         2426 c         2384 g         1145 t
ORIGIN      
        1 cttgccctgc gccagggcgg gcatcctttc gcaaaaggat tcagcggcga cgccctgcgc
       61 ggccgctaga gcagcaccag gttgtccctg tggatgagct ccggctcatc cacgtagccg
      121 agcaccgact cgatctcgcc cgagggccgg cgcatgatgc gccgcgtctc ttcggccgaa
      181 tagttgacca gcccgcgcgc gacttcgcgt cccgcgggat cgaggcagcc gacgatctcg
      241 ccacgcgcga actcgccggt aacttcgacc accccgatcg gcagcaggct cttgccatcg
      301 cggaccagcg ccctgaccgc gccagcgtcg agccgcaggc agccgctgac cgtgagatgg
      361 tcggcgagcc actgcttgcg cgccgcaatc ggcgcggtcc gggcggtgag cagcgtgccg
      421 atgagctcgc cctgcgcgag gcgcaccagc acctcgggct cgcgcccgga agcgatcacg
      481 gtgtgcgcgc cgctgcgcgc ggcgcgccgg gcggcgagca ccttggtgag catgccgcct
      541 ttcgccagcg cggtcccggt gccgcctgcc atcgcttcca gccgcgcgtc gtcagcgtct
      601 gcggtctcga ccagtttcgc ctggacatta ctgcgcgggt cggactcgta cagccccggc
      661 tgatcggtga ggatgaccag cgcgtcggcc tccaccaggt tagtgaccag cgcgccgagg
      721 gtgtcgttgt cgccgaactt gatttcgtcg gtgaccacgg tgtcgttctc gttgatcact
      781 ggaatgacgc ccagggcgag cagggtgcgc agcgtcgagc gcgcgttcag atagcgctgc
      841 cggtcggcca tgtcggcatg agtcagcagg atctgcgcgg tgtgcaggcc gtgctcgcga
      901 aagcagctct cgtagcactg cgctagcccc atctggccca ccgccgccgc agcctgcagc
      961 tcgtgcatgg tgtgcgggcg cttcgcccag cccaggcgct gcatgccttc ggcgatcgcg
     1021 ccgctcgata ccaggaccat gcgccggccg aggcttcgca ggcgcgcgat ctgcgccgcc
     1081 caggtcgcga tcgccgcgac gtcgagcccg cggccggcgg tggtcaccag gctcgagccg
     1141 accttgacca ccagggtggt cgccttttgt agcggagagc tcattttttc gcagccagct
     1201 cgcgggcaat cgccttgcag acctcgcggc agcccttgcc gctgatcgcc gataccttga
     1261 accatggccg cttccagcgc aaggcgcgca ggatgctcgc gatgcgcttg tcggcgtcct
     1321 cggcgaggtc gatcttgttg aacaccagcc agcgcggctt gcgataaagg gcggcgtcgt
     1381 acttcttcag ctcaagggca atcgctttcg cgtcgcacgc cgggtcggcg cccgggtcga
     1441 agggcgcgac gtccacgagg tgcaggagaa tgcgggtgcg ggccagatgg cgcaggaact
     1501 ggtgccccag ccccgcgccc tcggccgcgc cctcgatgag gccgggcacg tcggcgacca
     1561 cgaagcttct ctcgccgacg cgcaccacgc cgagcgacgg attgagcgta gtgaacggat
     1621 aatccgccac tttcggccgg gcactggaga ccgcccggat gaaagtggac tttccggcgt
     1681 tgggcaggcc cagcaacccg acgtcggcga gcacgcgcag ctcgagcgca agcgaacggc
     1741 tctcccccgg ctcgcctttc gtgaactgcc gcggcgcgcg gttcacgctt gagcggaagt
     1801 gcaggttgcc aagcccgccg ctgccgccgc gtgccaacag cgcccgttcg ccgtccatcg
     1861 tcaagtcggc gacccaatcg ccgctctctg catcgcggat cacggtgcct accggcatgc
     1921 gcagtacgac gtcctcgccc gagcggccgt tgcaatccga gcccatgccc ctggcgccgt
     1981 gagcggcgcg gtggatgcgc gcgaagcggt aatcgatgag cgtgttgacg ttgcggtcgg
     2041 cgcgcgccca gacgctgccg ccgcggccgc cgtcgccgcc gtccggcccg ccgcgcggca
     2101 cgtacttctc gcggcggaac gacgcggagc cgtcgccgcc cttgccggca tgcacctcga
     2161 ttttcgcctc gtcgatgaat ttcatggccc gaaaacaaaa aagccctacc ggtctcgata
     2221 gggctttccg tgtaagaagg accggcctag gccggaatca cgctgacggt atttctctcg
     2281 tcctcgcctt tgaccgcgta ctgcacctgc cccgtgacgc gcgcatacag cgtgtggtcg
     2341 cgcccgatgc cgacattgac gccgggatgc acccgggtac cgcgctgccg gacgagaatc
     2401 tgccccgcgt ttacccgctc gccgccgtag gccttcacgc cgagccgttt cgcctgggaa
     2461 tcgcgcccgt tgcgtgagct gccgcctgct ttcttgtgtg ccatgcctag ccttggatga
     2521 tgtcgtcgat gcggacctcg gtatacgact gccggtggcc ctgcgatttc tggtggtgct
     2581 tgcgccggcg cagcttgaag atgcgcacct tgtcgccgcg cccgtgcgac accaccgtcg
     2641 ccttcaccag cgcgccggac accagcggcg ccccgacctt taccgcgtcg ccgctgccga
     2701 ccagcagcac ctcgtcgaac gaaaccgcgg cgccgacctc ggccgaaagc gcctcgacgc
     2761 gaagcttggc gccggactcg acccgatatt gcttgccgcc ggacttgatc actgcgtaca
     2821 ttgtgagttg tggtaagccg cggaaaaaga ccaggaatta tagacgaatc atgagtttcg
     2881 tgtccatgct ggctccggtc gcggacgaca tgcgccgcgt cgacgagctg atcgggcgcc
     2941 gcctggactc cgacgtcccg ctggtgcgcc aggtggccga atacatcgtc gcctcgggag
     3001 gcaagcggct gcgcccggcg ctggtcttgc tcgcctgcgg cgccgccggc ttcagggaga
     3061 aaagcggcgg cgagctgcgc ctgcggctcg cggcggtggt ggagttcatc cataccgcga
     3121 cgctgctgca cgacgatgtg gtcgacgcgt cgcacctgcg gcggggacgg cgcaccgcga
     3181 acgccgcgtt cggcaacgcc gcggcggtgc tggtcggcga tttcctctat tcgcgcgcct
     3241 tccagatgat ggtcgaggtc gacgacatgc gcgtgatgcg cgtgctcgcc gacgcgacca
     3301 acaccatcgc ggcgggcgag gtgatgcagc tgatgggcag ccacgacccc gaggtcgacg
     3361 aagcacgcta cctggaggtg attcgccgca agacggcgaa gttgttcgag gccgcggcgc
     3421 gcctgggcgg cgtgctggcg cgcagcccgg gcagcgtcga ggacggcctc gcgggctacg
     3481 gcatgcacgt cggcactgcg ttccagctga tcgacgatgt cctcgattac tcgggcgacg
     3541 aggcgaccat cggcaagagc ctcggcgacg accttgccga aggcaagcct acgcttcccc
     3601 tgatccgcgt gatgcgcacc gggacgccgc agcaggcggc gatggtgcgc cgcgcgatcg
     3661 tcgacggcgg ccgcggcgag ttcgccggcg tgctgcaagc gatgcgcgcc tgcggcgcgc
     3721 tcgactacgc gcgccaggcc gcgcagcgcg aagccgacgc cgcgagcgca gcgctggcgc
     3781 cgctggccgc atcggagttc aagcaatctt tgctagaatt ggcgtccttc tcggtgacgc
     3841 gcagcagcta gtttttcctt cgtgtcgggg tgtagctcag cctggtagag tactgcgttc
     3901 gggacgcagg tgtcgctggt tcaaatccag tcaccccgac caccaccttc tcatgcggcg
     3961 gcgccgccga cgactcgcag cagctcggtg atgcgcgccc ggtaagaatg atcgcgctgg
     4021 aacgaggccg atgctgccaa agcgcgaccc cgcagctcga cggcatcttc ggtggctttc
     4081 cgggcaagac gcagcagttc gccgtcgtcg cggtaaatca gcatcccggc acgctgcgcg
     4141 gggtcgaaga tctcgtcgag ctccttcttg taatcgacga gctgcggcac gccgcaggcg
     4201 gcggcctcga acacccgcgg gttgagcccg tgatcgaagc ggccgcgcca agtgtggatg
     4261 ttgagcgtcg ccttgcaggc ggcaaagatc tcggccatgc gctgcggcga gaaaaagccg
     4321 tgctcgagcc gcgcgcgcac cgccgagtgc ttcgcgagct tgcgtctcca ggggccgaac
     4381 acgcgcacgt cgagccccgc gtgcagcagc gcgtcgacga gctgctcgcg catcgccgac
     4441 cagtcgcccg caaaggagat gtcacacagg ctcgcgcgcc cgctcagcgg cctgtggacc
     4501 gcgggctcgc acgccgtggg cagaaaatga acgttcttca ggccgagcgc ggcgtaggct
     4561 tcaaccgagt agcgcgcgtt ggtaaacgcg aagtcgtagc caggcaggat cttcgctccg
     4621 cgctcaaact gaaaggggtc gttgagccac cagttcgctg tcacaacgcc gcgcttgcgc
     4681 agttcagcga acacttgcgc attcacattc gctccgtaaa gcgtaaggaa gagttgcggg
     4741 cgcgcttctt caatcgcctg gagcaggcgc ggtgcaaccg catcccctgc ggaacgcttt
     4801 ctcagaagtc gcgatttgag cttttccgcg agcgccagcg aatagtcggc gaccgagacc
     4861 tggtgcccga gcgccgctgc cgcctcggga acgaaccggc tcatcggcac cgtgcgcagg
     4921 cgtcctggga ccgctacgag tatcttcatc cgggttccgt tagataatca tcccatgggc
     4981 cgccttgtcg tcatcgtgct gctcgtcgtg gtcgccgtct ggctcctgcg gcgtgccctg
     5041 cgccgccccg gccaggggcc cgacagcgca ggcaagcctg ccgccccggg cgagctggtg
     5101 cgctgcgcgc attgcggcat gcacctgccg ctcgccgagg cgcacgcgcg cgacgaccgc
     5161 gtctactgca gcgaagagca tgcccgtctc ggaccgggga gggcgtgatg gacgcgccgc
     5221 tgcgctatcc ggagctgttc acgccgccgc gcaagcgctc tcccgagtcg ttctggatct
     5281 cgctcggcta tttcaacctc taccgcgtcg cggtggcgac gctgtttttc gccatcacca
     5341 tggtgtacgg cgatgcgctc aatctcggct cccactccct ggtcctgttc cgctacgtct
     5401 gcgcggcgta cctgcttttc ggcatcgcgt tccagggcgt gctgcgcagc ctgcgcgagc
     5461 attttaatct gcagctgacg ctgcacgtca gcctggacat cgccgccatc acgctgctga
     5521 tgtatgcgag cggcggtgtg cgcagcgggc tcggcgtgat gctgctgatc acgctcaccg
     5581 gcgcggcgat cctcgcgccg cggcgcttca gttttctgta cgcggcgctc gcgaccatcg
     5641 cgctgctcct cgagcagggc tactggaccc tggcgcatga cgcgccgacc agcaacttcc
     5701 tgcagcccgg tctggtggcg atgggctgct tcgccggcgt gggaatcacc ggctggctgg
     5761 cgcagcgcgt ggcgaccaac gagcgcctcg cgcggcagcg cggccgcgag ctcgcgatgc
     5821 agacgcgcgt caaccagctg gtgctccagg acacgcacga cggcgtggtg gtgctcgacc
     5881 gcgacggccg ggtggtgcaa ttcaatcccc aggcgcagcg gctgctgcgc gccgagcggc
     5941 tgctcggcgg cgagatggct gacctgctgc cgggattcga ccagcactgg cgcgcctggc
     6001 gctctggcgg gaggggggag ccgagccccg aggtcgagct gcgcgggcgc gtgatgcggc
     6061 tgcgcctgct cgacaccggc accgaggagg gattcgcggt gctgttcatc gaagacacga
     6121 cgcgcacccg cgagcaggcg cagcagctca agctcgcggc gctcgggcgc ctgacggcga
     6181 atatcgcgca cgagatccgc aacccgctcg ccgcgatcag ccacgcggcc gagctgctgc
     6241 acgaggagaa gcgcgccgag gaccgcgggc gactgacgcg catcgtgcat gacaacacgc
     6301 agcgcctcga gcggctggtg tcggacgtcc tgcaactcag ccggcgcgac cgcattgccg
     6361 ccgagccgat ccgcgtcggg gtctggctcg ccggctttgt cgacgagttc gtcgccaacg
     6421 agtcggtcgc cgccgggcgc ttcgtcaccg acgtcgccgg cgatccgtgg atccgcttcg
     6481 accgcgagca tctcagccag gtgatgtgga acctgctgcg caacgccatg cgctatgcgc
     6541 gcgccgagcc gcacagcgtg cgcatcgcgc tgcgcgagtt cgccgaccgc gtagaattga
     6601 gcgtcatcga taacggcccg ggcgtgccgc tgtcgatcca gggacagctg ttcgagccgt
     6661 tttttaccac cgaagcgaaa ggcaccggac tggggctgta cctggcgcgc gagctgtgcg
     6721 cgacgaatgg cgccaccctc gaatacgtcg gcgacatgcc gggagcccat ttcaggattc
     6781 agtgccggga ggcgcgcgtc gcatgaagcg tgcggagcga gccgcagcgc cgcgcggcgc
     6841 gcaggtgctg gtggtcgacg acgagcccga catccgcgag ctgctcgagc tgacgctggt
     6901 caagatgggc ctcggcgtgc aggccgtcgg atcgatcgcc gaggcgaagg agtgcctgaa
     6961 gagcgcccgc tacgacctgt gcctcaccga catgcggctc gccgacggcg aggggc
//
LOCUS       DASPXW010000305         4442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_594737, whole genome shotgun sequence.
ACCESSION   DASPXW010000305 DASPXW010000000
VERSION     DASPXW010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594737"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..1546
                     /locus_tag="VEL04_06945"
     CDS             35..1546
                     /locus_tag="VEL04_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HYT48140.1"
                     /translation="MELFASLWMGFVVAVSPINLLYAFIGVLLGTLIGVLPGIGPVAT
                     IAMLLPVTFNLNPLAALIMLAGIYYGAQYGGSTTAILVNIPGESSSVVTCLDGYQMAR
                     QGRAGPALAVAALGSFFAGCVATLIIAVAAPPLAEVALKFGPSEYFSLMVFGLIAATV
                     LAHGSLVKAIAMVVWGLLFGIVGTDVNSGVLRFTFDIPELSDGIGFVIVAMGMFGTTE
                     IILNLEMKEKREIFTSKVKNLWPSKDDFKRAWPAVLRGTALGSALGILPGGGALLASF
                     GAYTVEKKMSRHPEQFGKGAIEGVAGPESANNAGAQTSFIPMLTLGIPGNAVMALMIG
                     ALMIQGIAPGPQVMSEKPQLFWGLIASMWVGNLMLVVLNLPLIGMWVKLLTVPYRYLY
                     PSILVFMAIGVYSLSNNPFDVLIMAVFGILGYVCAKLECEPAPMILGFILGPLMEENL
                     RRAMLLSRGDPFVFISPSKPISLAFLLASLILLVMVGLPAIRKRREEVFVEET"
     gene            complement(1557..1940)
                     /locus_tag="VEL04_06950"
     CDS             complement(1557..1940)
                     /locus_tag="VEL04_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HYT48141.1"
                     /translation="MAEVRIHNVEALGKPLGQYSHVARVKAGEFLFIAGMLAAGEDFD
                     AQCTKVYGQIGEALKSANAGWASVVQFTTYLVHSQDIPRFMQWRLREFPQLFPGGAYP
                     PNTLLVIDRLVAEQYLIEVQTVAAL"
     gene            complement(1933..2376)
                     /locus_tag="VEL04_06955"
     CDS             complement(1933..2376)
                     /locus_tag="VEL04_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYT48142.1"
                     /translation="MASRAQEELKPRHRVLIVDDEESTRLLLARMLSKELKLDAQLAG
                     TCEQALRLAGNYAYDAILLDLLMPGIGGLGVLRRIRSGSPNAATPVIVVSVAVDQATI
                     ASCMAAGANAHHAKPIRRAELIATVKAQLAARGKPKTTGGGESHG"
     gene            complement(2349..2903)
                     /locus_tag="VEL04_06960"
     CDS             complement(2349..2903)
                     /locus_tag="VEL04_06960"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HYT48143.1"
                     /translation="MGHRLSKITTRTGDAGETGLGDGSRVPKDSPRVAALGDLDELNS
                     AIGVLLAEELPQEVRAALAEVQQHLFDLGGEVSIPGHAMLTSQQVEHLEQRLAAWNAG
                     LPPLKEFVLPGGTRAAAAAHLARTVCRRAERGLVSLAHEEKLSEHSRRYLNRLSDLLF
                     VVARLLNRYAGRDDIQWRHERKKS"
     gene            complement(2911..4137)
                     /gene="xseA"
                     /locus_tag="VEL04_06965"
     CDS             complement(2911..4137)
                     /gene="xseA"
                     /locus_tag="VEL04_06965"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HYT48144.1"
                     /translation="MQNDSPPHPAPAAAGVLTVSELLRSVRDTLERRFPLLWVRGELS
                     NLTRAPSGHCYFTLKDGAAQVDCVMFRSRAALLERELRDGAQVEARVLVTLYEPRGRF
                     QLSVEAVRRAGLGPLYERYLRVKDQLEREGLFDAALKRELPRFPRSIGVVTSRAAAAL
                     RDVLTTLARRNPAIEVIVYPVPVQGDGAAARIAAMLARASARGECDVLLLVRGGGSIE
                     DLWQFNEEVVARALRASRIPVVVGVGHETDFTIADFAADARAPTPTAAAELASPLRAE
                     LLGRVAAGARSIAREMRRRLEYAIQTVDGCARRLLHPAERLRSSGSLVAQLSARLGFA
                     FIHAIQACGSKLERLRATLDGLDPQAVLARGYSITYNAAGEVLRDAALARDGERLHTT
                     LARGRLESEVRKKEIL"
BASE COUNT          692 a         1506 c         1481 g          763 t
ORIGIN      
        1 cgccaactgc ccgatcaggt aaggagctag agcgatggaa ctcttcgcca gtctgtggat
       61 gggcttcgtc gtcgcggtgt cgccgatcaa cctgctgtat gcgttcatcg gcgtgctgct
      121 cggcacgctg atcggcgtgc tgcccggcat cgggccggtg gcgaccatcg ccatgctgct
      181 gccggtcact ttcaacctga acccgctggc ggcgctcatc atgctcgccg gcatctacta
      241 cggcgcgcag tacggcggct cgaccaccgc catactggtg aatatcccgg gcgaatcgtc
      301 ctcggtggtc acttgcctcg acggctatca gatggcgcgg cagggccgcg ccggcccggc
      361 gctcgcagtg gcggcgctcg gctcgttttt cgccggctgc gtcgcgacgc tgatcatcgc
      421 ggtggcggcg ccgccgcttg ccgaggtggc gctcaagttc ggcccctccg agtacttctc
      481 gctgatggtg ttcggcctga tcgcggcgac cgtgctcgcg cacggctcgc tcgtcaaggc
      541 gatcgcgatg gtggtctggg gcctgctgtt cggcatcgtc ggcaccgacg tcaactcggg
      601 cgtgctgcgc ttcaccttcg acatccccga gctttccgac ggcatcggct tcgtgatcgt
      661 cgccatgggg atgttcggca ccaccgagat catcctcaac ctcgagatga aggagaaacg
      721 cgagatcttc accagcaagg tgaagaatct ctggccgagc aaggacgact tcaagcgcgc
      781 ctggcctgcg gtgctgcgcg gcaccgcgct cggctcggcg ctcggcatcc tgccgggcgg
      841 cggcgcgctg ctcgcctcct tcggcgccta taccgtcgag aagaaaatgt cccgccatcc
      901 ggagcagttc ggcaagggcg cgatcgaggg cgtggcggga ccggagtcgg cgaacaacgc
      961 cggcgcgcag acctcgttca tcccgatgct cacgctgggc atcccgggca acgcggtgat
     1021 ggcgctgatg atcggcgcgc tgatgatcca gggcatcgcc ccggggccgc aggtaatgag
     1081 cgagaagccg cagctcttct ggggcctgat cgcctcgatg tgggtcggca acctgatgct
     1141 ggtggtgctc aacctgccgc tgatcggcat gtgggtcaag ctgctcaccg tgccctaccg
     1201 ctacctctat ccctcgatcc tggtgttcat ggcgatcggc gtctacagcc tgtcgaacaa
     1261 ccccttcgac gtgttgatca tggcggtgtt cgggatactg ggctacgtct gcgccaagct
     1321 cgagtgcgag ccggcgccga tgatcctcgg cttcatcctc gggccgttga tggaggagaa
     1381 cctgaggcgc gcgatgctgc tgtcgcgcgg cgacccgttc gtgttcatca gcccttcgaa
     1441 gccgatcagc ctggcgttcc tcctcgcctc gctgatcctg ctcgtcatgg tcgggctgcc
     1501 cgcgatccgc aagaggcgcg aggaagtgtt cgtcgaagag acctagcgcg gccgacctag
     1561 agcgcggcga cggtctgcac ctcgatcagg tactgctcgg cgaccagccg gtcgatgacg
     1621 agcagcgtgt tcggcgggta ggcgccgccg gggaacagct gcggaaattc gcgcagccgc
     1681 cactgcatga acctgggaat gtcctgcgag tgcaccagat aggtggtgaa ctgcaccacg
     1741 ctcgcccagc cggcgttggc cgatttcagg gcctctccga tctggccata caccttggtg
     1801 cactgggcat cgaagtcctc gcccgccgcg agcatgccgg cgatgaacag gaactccccc
     1861 gccttgacgc gcgccacgtg cgagtactgc ccgagcggct tgcccagcgc ctcgacgttg
     1921 tggatcctga cctcagccat ggctttcacc tcccccggtc gttttcggtt tgccgcgcgc
     1981 cgcaagctgc gctttcaccg tggcgatgag ctcggcgcgc cggatcggtt tggcatgatg
     2041 ggcgttggcg ccggctgcca tgcagctcgc gatcgtcgcc tggtcgaccg ccaccgagac
     2101 gacgatcacc ggcgttgccg cgttgggcga ccccgagcgg attctcctga gcacgccgag
     2161 gccgccgatg ccgggcatca ggagatcgag caggatggcg tcgtaggcgt agttgccggc
     2221 cagcctcagc gcctgctcgc aagtcccggc gagctgcgcg tcgagcttca gctccttcga
     2281 gagcatgcgc gcgaggagca gccgggtcga ttcctcgtcg tcgacgatca acacccggtg
     2341 acggggcttc agctcttctt gcgctcgtga cgccattgaa tatcgtcgcg tccggcgtac
     2401 cggttgagca ggcgcgccac gacgaacagc aggtccgaca ggcggttgag gtagcggcgc
     2461 gagtgttcgc tcagcttttc ctcatgcgcc aatgagacga ggccgcgctc cgcgcgccgg
     2521 cataccgtgc gtgcgaggtg cgccgcggct gcggcgcgcg tgccgccggg gaggacgaat
     2581 tccttgagcg gcggcaggcc ggcgttccag gccgcgaggc gctgctcgag atgctccact
     2641 tgctgggagg tcagcatggc gtgtcccgga atgctgactt cgccgccgag atcgaacagg
     2701 tgctgctgca cctcggcgag cgcggcgcgc acttcctgcg gcaactcctc ggcaagcagc
     2761 acgccgattg ccgaattgag ctcgtcgaga tctcccagcg cagccacccg cgggctgtcc
     2821 ttcggcacgc gcgagccgtc gcccaggccc gtctctcccg cgtcgccggt gcgcgtggtg
     2881 atcttggaga gacgatggcc catgtccgga ttataggatt tctttcttcc tgacttcgct
     2941 ttccaggcgg ccgcgcgcca gcgtcgtatg caggcgctcg ccgtcgcggg caagcgccgc
     3001 atcgcgcagc acctcgcccg cggcgttgta ggtgatgctg tagccgcgcg cgagcaccgc
     3061 ttgggggtcg agcccgtcga gcgtcgcgcg cagacgctcg agcttcgatc cgcaagcttg
     3121 gatggcgtgg atgaaagcaa agccgaggcg cgcggagagc tgcgctacca ggctgccgga
     3181 cgagcgcagg cgctccgccg ggtgcagcag gcgccgcgcg cagccgtcta ccgtctggat
     3241 ggcgtactcg agccgccgcc gcatctcgcg cgcgatgctg cgcgcgccag cggcgacgcg
     3301 gccgagcagc tcggcgcgca aggggctcgc gagctccgct gccgcggtcg gcgtcggtgc
     3361 cctcgcatcg gcggcaaaat cggcgatggt gaaatcggtc tcgtgcccga cgcccaccac
     3421 caccgggatg cgcgaagccc gcagcgcgcg cgccaccacc tcctcgttga actgccacag
     3481 gtcctcgatc gagcctccgc cgcgcaccag cagcagcacg tcgcactccc cgcgcgcgct
     3541 ggcgcgcgct agcattgcgg cgatgcgcgc cgcagcacca tccccctgca ccggcaccgg
     3601 atagacgatc acctcgatcg caggattgcg tcgcgccagc gtggtgagca cgtcgcgcag
     3661 cgccgccgca gcgcgcgagg tgaccacgcc gatcgagcgc ggaaagcgcg gcagctcgcg
     3721 cttcagcgcc gcatcgaaca ggccttcgcg ctcgagctgg tccttgacgc gcaggtaacg
     3781 ctcgtagagc ggtcccaggc cggcgcggcg caccgcctcg acgctgagct ggaagcggcc
     3841 gcgaggctca tagagcgtca ccagcacgcg cgcttccacc tgcgcgccat cacgcaactc
     3901 ccgctcgagc agggccgcgc ggctgcgaaa catcacgcaa tcgacctgcg cggcgccgtc
     3961 cttgagggtg aagtagcagt gcccggaggg cgcgcgcgtg agattggaca gctcgccgcg
     4021 cacccacagc agcggaaagc gccgctcgag cgtgtcgcgc acgctgcgca gcaattcgga
     4081 taccgtgagg acgcccgccg ccgcaggcgc aggatgaggt ggcgagtcgt tctgcatctg
     4141 tgtcaagagc gcaagcgaga ctacactatc cacatgcggg gccgggccgc ccggggcctc
     4201 gggaattccc ccgcggaccc tgattctttg cgctaaattg ctgttcggac ggaacaaaag
     4261 actgctcaaa ttttgatcgc gaccgtctcg acgcgtgccg caactacgtc gaatgtgtct
     4321 atacataaaa aatccacaaa cttatccaca ggtttttgtg gatatcgcgg aatggactaa
     4381 gggcagccgc tttacagcag cgcgagcgat gcggatgttg cgctgcgcaa acttgccgca
     4441 gc
//
LOCUS       DASPXW010000306         2628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1601285, whole genome shotgun sequence.
ACCESSION   DASPXW010000306 DASPXW010000000
VERSION     DASPXW010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1601285"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1333
                     /locus_tag="VEL04_06970"
     CDS             <1..1333
                     /locus_tag="VEL04_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HYT48145.1"
                     /translation="KPAPDAKPNGGGSSHPAASGDSNHEELRRWAEPPPPAAKANGVH
                     APAAKASAAALPVAARAANGHANGHAAPAAVAASKQPSLSTTMAYAAAAARAPAERSI
                     VPPPAPSTPVVIQDPAKGSVELVLDDVLYGEPPPPALHGTFTASDHAALQSTFEDLAV
                     PHMAEVRSVMMELQWGEVQSSWLELARPALRSLRKMAEPVGNLPLCEALDKFDAALAE
                     VLGPGQPPEPSGPARDALLAAYAALRTCLPRAFDLDGERDRREPLIVAALLEQVPGLE
                     PLMIEKMLEAGLGKLSALYAARADEIAAVTGIPTAVAVATAARIQAFRSATPAGLATV
                     DTASTLRELSHLLDTLRAQHAAFEEAARGWSDSERKSKKQLRRDRQVSFLQVTIELVR
                     LGEVDLAQRLGRLPFAKKIDEVDRMVGQAVMSAKVKDRAPARTMAGAGAAT"
     gene            1354..2403
                     /locus_tag="VEL04_06975"
     CDS             1354..2403
                     /locus_tag="VEL04_06975"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HYT48146.1"
                     /translation="MGELTRDQVLQKLRKNERLERADLRGIDLSKAAMPGAMLSRADL
                     EGANLEAAVLRKANLKNANLREAYLGGCDLQEANLENADLEGAKLDRANLAGANLARA
                     NLDGASLAGANLQGARLSFAQVSSANLTGADLRNAVLTHANFEESSLGAVKATQADLR
                     AANLRGATLTKADLTGAKVAGLIGTGSPLGDVLASWVDTSEAGDGTGRITNGEIPGLL
                     TGLRAAAVATAPASRRYFGHGDVLKNATLEFEAGARVEIDSLFENCAINLGEGTELVV
                     GETGILADCKITGAGSITVHGHFFERESPGIVGPRELLVSSGGALVASVEQNEALTRF
                     GFERGSRLRVKIVKQ"
BASE COUNT          425 a          887 c          963 g          353 t
ORIGIN      
        1 caagccggct cccgacgcca agccaaacgg tgggggcagc tcgcaccccg cagcaagcgg
       61 cgattccaat cacgaggagc tgcggcgctg ggccgagccg ccgccgccgg ccgcgaaggc
      121 gaacggcgtg cacgcgcccg ccgcgaaggc gtcggcagcc gccttaccgg tcgctgcccg
      181 cgccgccaac ggtcacgcca atgggcacgc cgcacccgcg gcggtggcgg cgtcgaagca
      241 gccttcactc tccaccacga tggcatatgc ggcggccgcc gcgcgtgcgc ccgccgagcg
      301 gtcgattgtt ccgccgcccg cgccttctac gccggtcgtg attcaggatc ccgccaaggg
      361 ctcggtcgag ctggtgctcg atgacgtgct ttacggcgaa ccgcccccgc cagccctgca
      421 cggaaccttt accgcgtcgg atcatgccgc gctgcagtcg acgttcgagg atctcgcggt
      481 gccgcacatg gccgaggtcc gcagcgtgat gatggagctt cagtggggcg aagtgcagtc
      541 gagctggctc gagctcgccc ggcctgcgtt gcgctcgctg cgcaagatgg ctgagccggt
      601 cggcaacctc ccgctctgcg aggcgctgga caagttcgac gccgcgctgg ccgaggtgct
      661 cgggccaggg cagcctcccg agccgtcggg ccccgcgcgt gacgcgctgc tggcggccta
      721 cgcggcgctg cggacctgcc tgccgcgcgc gttcgacctg gacggcgagc gcgatcggcg
      781 cgagccgctg atcgtggccg cgctgctgga acaggtgccg gggctcgagc cgctcatgat
      841 cgagaagatg ctcgaggcgg ggctcggcaa gctgtcggcg ctctacgccg cgcgcgccga
      901 cgagatcgcc gccgtcaccg gcatcccgac cgccgtcgcc gtggcgaccg ccgcccgcat
      961 tcaggcgttc cggagcgcga cgcccgccgg gctcgccacc gtggacaccg cctcgacgct
     1021 gcgcgagctc tcccacctgc tcgacacgct ccgcgcgcaa cacgccgcgt tcgaagaggc
     1081 ggcgcgcggg tggtcggatt ccgagcggaa gtcgaagaag cagcttcggc gagaccgaca
     1141 ggtgtcattc ctgcaagtta cgatcgagct cgtgcgcctg ggcgaggtcg acctcgccca
     1201 gcggctcggc aggctgccgt tcgcgaagaa gatcgacgag gtcgatcgga tggtcggcca
     1261 ggccgtgatg tcggccaagg tgaaagaccg ggcgccggcc cggaccatgg cgggcgccgg
     1321 cgcggcgacc tgaacagaga ggaaccaagc gacatgggtg agctgacacg cgatcaggta
     1381 ttgcagaagc tcaggaagaa cgagcggctc gaacgcgccg acctgcgcgg catcgatctc
     1441 tcgaaggcag cgatgccggg tgcgatgctg tcgcgcgcgg acctcgaggg cgcgaacctg
     1501 gaagcggcgg tgctgcgcaa agcgaacctc aagaacgcca acctgcgcga ggcctacctc
     1561 ggtggctgcg atctccagga ggccaacctc gaaaacgcgg atctcgaggg cgcgaagctc
     1621 gaccgcgcga acctcgcggg cgcgaacctc gcgcgcgcca acctcgacgg cgccagcctg
     1681 gcgggcgcga acctgcaagg cgcgcggctg tcgttcgcgc aggtgagctc ggcgaacttg
     1741 accggcgcgg acctgcgcaa cgccgtgctc acgcacgcga acttcgagga gtcgtcgctg
     1801 ggcgcggtca aggcgacgca ggcggacctg cgcgcggcca acctgcgcgg cgcgacgttg
     1861 accaaggcgg acctcaccgg cgccaaggtc gcgggtctca tcggcaccgg cagcccgctc
     1921 ggcgacgtgc tcgcgtcctg ggtcgacacg agcgaagccg gcgacgggac cggccgcatc
     1981 accaacggcg agattccagg tctgctcacg ggcttgcgcg cggccgcggt ggcgaccgcg
     2041 cccgcgtcgc ggcgctactt cggtcacggc gacgtgctga agaacgcgac gctggagttc
     2101 gaggcgggtg cgcgggtcga gatcgacagc ctgttcgaga actgcgccat caacctcggc
     2161 gagggcaccg agctggtcgt gggcgaaacc ggcatactcg cggactgcaa gatcaccggc
     2221 gccggctcga tcaccgtgca cggtcacttt ttcgagcgcg agagccccgg catcgtgggc
     2281 ccgcgcgagc tgctggtttc gagcggcggc gcgctggtgg cgtcggtcga gcagaacgag
     2341 gcgctgacgc ggttcgggtt cgagcgcggc agtcggttgc gcgtgaagat tgtgaaacag
     2401 tgatcgcgtg ggggaagggc cagctttgct ggaccgagcc agcgcgggag gttcggaacc
     2461 ctttgagggt tccgagtaca gtgaaaggcg gacgacgatg agcgattcga agaagaccct
     2521 gatcgaagag ggcaccgagc tgaaggggac gctgacctcg acgtgtccga tcgtcgtgca
     2581 gggtagggtc gaaggtgacc tgacgggccc gtcggtcgag gtgaccga
//
LOCUS       DASPXW010000307         4268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_215460, whole genome shotgun sequence.
ACCESSION   DASPXW010000307 DASPXW010000000
VERSION     DASPXW010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215460"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..916
                     /locus_tag="VEL04_06980"
     CDS             <1..916
                     /locus_tag="VEL04_06980"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYT48147.1"
                     /translation="RRIAGSDALVAIGPTCSNVAEPLFGILQKKVDDNSDSGLQMPVF
                     TDVAIKVGLARISEWAFRNVPNEATMYSSLFYWLKTKYPNAQTVYGGVEEDFAHSRAS
                     WYAVMKERAEANGYKVLGNSKWLLNDANFATQVREMKNANADIVAISAHPFTTCGVLK
                     EMQRQGVKPKLLVGLTSSSSLETLQGCAKQAEGIIIPTSFAPVTKEGKAAAEITARFK
                     GAADLHSMAAWENMFILRQVMESEKISGDKSQVQADRRKIRDGLAKLKETKGLMGVTK
                     RTEDREADKPYLFVHASKGEWKVLYNPL"
     gene            919..1788
                     /locus_tag="VEL04_06985"
     CDS             919..1788
                     /locus_tag="VEL04_06985"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYT48148.1"
                     /translation="MLDFLQAVADGVLFGATYALIGIGFTLVFGVMHKLNLAYAAASI
                     GGAYCGLLVLKVAAGAPAGVVFAAAFLAGGALGWLVYVTCFHFIPLSNPLAPLMSTMG
                     MLLLIDEVIVHATNGMPQNYPAVFDDVMLRVGGISVRGDLLLVFGVAVLCMAALLALL
                     YRTRLGLATRAVSQHAVAAQLCGIRVARVNAMTFVITGLLGGLAGALIGASVGALSPL
                     LAAPLTVKGLIVTVIGGLGSVPGAILAGLLVGGFENVFQQFRGVTERDLYVMLLLFAF
                     LVFRPGGLFQPKI"
     gene            1788..2651
                     /locus_tag="VEL04_06990"
     CDS             1788..2651
                     /locus_tag="VEL04_06990"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYT48149.1"
                     /translation="MDFYFGLAQTVGVHTLLGLSAYIVLLTGQVSLAQAGFFAVGAYA
                     AGMLTVLGGWHIVPALGAGAAAGAATACAVGFPALRVKGLMLVVATLAFGEGVRLFFF
                     NFDYQIAKGDIRIGPLGGEGFRQIRFFPEHGWTTAEVMLFMWLLVLLVMAALWWFDRS
                     RYGAVLRAVGEDEVAAQSCGINLTAIKVSAMTVGGAIAGIGGGLYAHYTTHIEHANFN
                     VLMATFALAYPIVGGLKSVFGTLAAVIFVQAFLIEGLRFLGDWRNLLFGALIVLAMNL
                     RPHGLFGRKRA"
     gene            2644..3384
                     /locus_tag="VEL04_06995"
     CDS             2644..3384
                     /locus_tag="VEL04_06995"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT48150.1"
                     /translation="MLELRGVSRHFGGVKAVERLDLSVTEGEIVGLIGPNGSGKSTTV
                     NLIAGLLPATSGEIVFRNTNLEKLPTHKRLQLGIARTFQNIRLFGQLSVWQNLWVAQN
                     APEDRARRSFLARWLGGQRGARAEIAELLQFSGLAHRQDELAANLAFGEQRRLELARA
                     LAARPKLLLLDEPAAGMNSEEIGQLRERILKLRARGVTIVLIEHVMELVMEVADRIAV
                     LNFGQKIAEGTPARVQADAVVKKAYLGG"
     gene            3389..4096
                     /locus_tag="VEL04_07000"
     CDS             3389..4096
                     /locus_tag="VEL04_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT48151.1"
                     /translation="MLSVRDLVTAYGKIEALKGVSLDVAAGSITCLLGPNGAGKTTLM
                     MTVAGVLRCRKGCIRFAGKDISSLQPHAIVARGIALVPENRLVFPNMSVLENLQAGGY
                     QRKDAIAEDIERLYARFPHLKERSSQQAGTLSGGEQQMLAVARALMSRPTLLLMDEPS
                     LGLAPMVVEEIFRIIAELHRDGTTIFLVEQNAHLALKLAQHFYLMEQGRVTFSGDPGS
                     LAEDEVIRRAYLGSSKG"
     gene            4107..>4268
                     /locus_tag="VEL04_07005"
     CDS             4107..>4268
                     /locus_tag="VEL04_07005"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYT48152.1"
                     /translation="MIDLLQNTLDGLTLGASYALLAVGFTLIFGVLRRANLCYGPAIM
                     FGAYLATWAS"
BASE COUNT          680 a         1416 c         1421 g          751 t
ORIGIN      
        1 gcgccggatt gccggaagcg acgcgctcgt cgcgatcggt ccgacctgct ccaacgtcgc
       61 cgagccgctg ttcggcatcc tgcagaagaa ggtcgacgac aacagcgact ccgggctgca
      121 gatgccggtg ttcaccgacg tcgcgatcaa ggtgggcctc gcgcgcatct cggaatgggc
      181 attccgcaac gtgccgaacg aggccacgat gtactcgtcg ctgttctact ggctgaagac
      241 caagtacccg aacgcgcaaa cggtctacgg cggcgtggaa gaggacttcg cgcattcgcg
      301 tgcctcgtgg tacgcggtga tgaaggagcg cgccgaggcg aacggctaca aagtgctcgg
      361 caactccaaa tggctgctca acgatgccaa cttcgccacc caggtgcgcg agatgaagaa
      421 cgcgaacgcc gacatcgttg cgatctcggc gcatccattc accacctgcg gcgtgctgaa
      481 ggaaatgcag cgccagggcg tcaagcccaa gctgctggtc ggcctcacca gctcctctag
      541 cctcgagacg ctgcagggct gcgccaagca ggccgagggc atcatcattc cgacctcctt
      601 cgcccccgtc accaaggaag gcaaggccgc tgcggaaatc accgccaggt tcaagggcgc
      661 tgccgacctg cattcgatgg ccgcgtggga gaacatgttc atcctcaggc aggtgatgga
      721 atcggagaaa atctccggcg acaaaagcca ggtgcaggcc gaccggcgca agattcgcga
      781 cggtctggcg aagctgaagg agacgaaggg attgatgggc gtgacgaagc gcaccgagga
      841 ccgcgaggcc gacaagccct atctcttcgt gcatgccagc aagggcgaat ggaaagttct
      901 ctataacccg ctgtaggggt gcttgatttc cttcaggcgg tcgccgacgg ggtccttttc
      961 ggcgccacct acgcgctgat cggcatcggc ttcacgctgg tgttcggcgt gatgcacaag
     1021 ctgaacctgg cgtacgccgc cgcttccatc ggcggcgcct actgcgggct gctggtcctc
     1081 aaggtcgcgg ccggcgcccc cgcgggcgtg gtgttcgcgg ccgccttcct cgccggcggc
     1141 gcgctcggct ggctggtgta cgtcacctgc ttccacttca tcccgctgag caatccgctc
     1201 gcgccgctca tgtcgaccat gggcatgctg ctgctcatcg acgaggtcat cgtccacgcc
     1261 accaacggca tgccgcagaa ctatccggcg gtgttcgacg acgtgatgct gcgcgtgggc
     1321 gggatcagcg tgcgcggcga tctgctgctc gtgttcgggg tcgcggtgct gtgcatggcc
     1381 gcgctgctgg cgctgctcta ccgcacccgg ctcggcctcg cgacgcgcgc ggtatcgcag
     1441 catgccgttg ccgcccagct ctgcggcatt cgcgtcgccc gggtgaacgc gatgaccttc
     1501 gtcatcaccg ggctgctcgg cggcctcgcg ggtgcgctca tcggcgcctc ggtcggcgcg
     1561 ctttcgccgc tgctcgccgc gcccctcacg gtcaaagggc tgatcgtcac ggtgatcggc
     1621 ggcctgggca gcgttcccgg cgccatcctt gccgggctgc tcgtcggcgg cttcgagaac
     1681 gtgttccagc agttccgcgg cgtcaccgag cgagacctgt acgtgatgct gctgctgttc
     1741 gctttcctgg tattcaggcc cggcggattg ttccagccca agatctgatg gacttctact
     1801 tcgggctggc gcaaaccgtc ggggtgcata ccctcctcgg cctgtcggcc tacatcgtgc
     1861 tcctcaccgg gcaggtgagc ctggcgcagg cggggttctt cgccgtcggc gcgtatgccg
     1921 ccgggatgct gacggtgctc ggcggatggc atatcgtgcc cgcgctcggc gccggcgcag
     1981 ccgcaggcgc cgcgaccgcg tgcgcggtcg gcttcccggc gctgcgcgtc aagggcctga
     2041 tgctggtggt cgcgacgctc gccttcggcg aaggggtgcg gcttttcttc ttcaacttcg
     2101 actaccagat cgccaagggc gatatccgca tcgggccgct cggcggcgag ggattccgcc
     2161 agatccgctt cttccccgag cacggctgga ccaccgccga ggtcatgctc ttcatgtggc
     2221 tgctggtgct gctcgtcatg gcagccctgt ggtggttcga tcgctcgcgc tacggcgcgg
     2281 tgctgcgcgc cgtgggcgaa gacgaggtgg cggcgcagtc ctgcggcatc aatctcacgg
     2341 cgatcaaggt aagcgcgatg accgttggcg gcgcgatcgc cggcatcggc ggcggcctgt
     2401 acgcgcacta cacgacgcat atcgagcacg ccaatttcaa cgtcctgatg gccaccttcg
     2461 cgctcgccta tccgatcgtc ggcgggctga agagcgtctt cggcaccctt gccgcggtaa
     2521 ttttcgtgca ggcgttcctc atcgaggggc tgcgcttcct cggcgactgg aggaacctgc
     2581 tgttcggcgc gctgatcgtg ctggcgatga acctgcggcc gcacggcctg ttcgggagaa
     2641 agcgtgcttg agctgagagg cgtgtcgcgc catttcggcg gcgtcaaggc cgtggagcgt
     2701 ctcgatcttt ccgtcaccga gggcgagatc gtcgggctga tcgggccgaa cggttcgggc
     2761 aagagcacca ccgtgaacct catcgccggg ctgctccccg ccacctccgg agagattgtt
     2821 ttcagaaata cgaacctgga aaagctgccg acgcacaaac gcctgcaact cggcatcgcg
     2881 cgcaccttcc agaacatccg cctgttcggc cagctcagcg tgtggcagaa cctgtgggtg
     2941 gcgcagaacg cgccagaaga ccgcgcgcgc cgcagcttcc tcgcgcgctg gctcggcggc
     3001 cagcgcggcg cgcgcgccga aatcgccgag ttgctgcagt tcagcggcct cgcccacagg
     3061 caggacgagc ttgccgccaa cctcgccttc ggcgagcagc gcaggctcga gctggcccgc
     3121 gccctggcgg cgcggccgaa gctcctgctg ctcgatgagc ccgccgcggg catgaacagc
     3181 gaggagatcg ggcagttgcg cgagcggatc ctcaagctgc gcgcgcgcgg cgtcaccatc
     3241 gtcttgatcg agcacgtcat ggagctcgtc atggaggtcg ccgaccgcat cgcggtgctc
     3301 aacttcggcc agaagatcgc cgagggaacg ccggcccggg tgcaggccga tgcggtggtg
     3361 aagaaggcct acctcggcgg ctagcccgat gctgagcgtg cgcgatctgg tcaccgccta
     3421 cggcaagatc gaagccctga aaggggtatc gctcgatgtc gccgcaggct cgatcacctg
     3481 cctgctcggg cccaacggcg ccggcaagac caccctgatg atgaccgtcg cgggcgttct
     3541 gaggtgcagg aagggctgca ttcgctttgc gggaaaagac atttcgtccc tgcagccgca
     3601 tgccatcgtc gcccggggca tcgcgctggt gccggaaaac cggctggtat tcccgaacat
     3661 gagcgtgctc gagaacctgc aggccggcgg ctaccagaga aaagacgcga tcgccgagga
     3721 tatcgaacgc ctgtacgcca ggtttccgca cctgaaggag cgttcctcgc agcaggcggg
     3781 tacgctctcg ggcggcgagc agcagatgct cgcggtggcg cgcgcgctca tgtcgcgtcc
     3841 gacgctgctg ctcatggacg agccctcgct cgggctcgct ccgatggtgg tggaggagat
     3901 cttccgcatc atcgccgagc tgcaccgcga cggcacgacc atcttcctgg tcgagcagaa
     3961 cgcgcacctg gcgctcaagc tggcgcagca tttctatctc atggaacagg gccgggtcac
     4021 gttcagcggc gatcccggct cgctggcgga ggacgaggtg atccgccgcg cctacctcgg
     4081 ctccagcaag ggctaaaagg caaggcttga tcgatctcct gcagaacacg ctcgacgggc
     4141 tgacgctcgg cgcctcctac gccctgctcg cggtgggctt caccctgatc ttcggcgtgc
     4201 tcaggcgcgc caacctgtgc tacggcccgg cgatcatgtt cggcgcctat ctcgcgacct
     4261 gggcgagc
//
LOCUS       DASPXW010000308         2219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1477615, whole genome shotgun sequence.
ACCESSION   DASPXW010000308 DASPXW010000000
VERSION     DASPXW010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1477615"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..1281
                     /locus_tag="VEL04_07010"
     CDS             46..1281
                     /locus_tag="VEL04_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYT48153.1"
                     /translation="MPEFYDALETRAPEMRERSLMAGLAGQIAHAKRRAPGWARILSE
                     VDPAAVTGRAALAGLPVTRKSDLVALQKAERPFGGLNATPVSQLGRIFVSPGPIYDPE
                     GRGRDWWRAGRALFAAGFRAGDLALNAFAYHFTPAGSMLESGALALGCVVVPSGTGQT
                     EMQVATIADLRASAYMGTPSFLKLIAEKADELKADIGSLKKAAVSAEYLPPALRAAMG
                     ERGIRVLQSYASADLGLIAYESEALEGMILDEGLILEIVRPGTGDPLPEGEVVEVVVT
                     TFNPDYPLVRFGTGDLSAVLPGASPCGRTNTRIRGWMGRADQTTKVKGMFVHPSQVAE
                     ILKRYPEVLKARLVVDNPGGNDRMTLRCEVRSADAGLAEKIAASLREITKLRGEVQFA
                     KLGELANDGKVIEDAKKYG"
     gene            1274..2080
                     /locus_tag="VEL04_07015"
     CDS             1274..2080
                     /locus_tag="VEL04_07015"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYT48154.1"
                     /translation="MARPGRLCILWLATLLAPLGAGAEEIVTVSGRPGATQSYLLLHY
                     ASPPEAVAVLFPGGEGLLSVRVEGDAVKFRGRENFLVRTRTLLRDKQVAVAVVDSPSD
                     RQRAGMDDGFRTGREHVEDIAAVVKDLKQRFPQAKIFLIGTSRGTLSAAYIGRALNNA
                     VDGVVLTSTVFYGGRRLGGIGLAGFDFAAIRARLLFVHHQDDACRACPYHPAENLGMT
                     YPLITVKGGKPAESDPCEPFAAHGYFGKEAETVAAIKRWMLGEAFPRTVE"
BASE COUNT          362 a          724 c          754 g          379 t
ORIGIN      
        1 cgtttgtgct atgttgccct gccgcgaacg ccagcttgcc cagcgatgcc cgagttctac
       61 gacgccctcg agacccgcgc cccggagatg cgcgagcgct cgctgatggc ggggctggcc
      121 ggccagatcg cgcatgccaa gcgccgcgcg cccggctggg cgcgtattct ttcggaggtc
      181 gatcctgccg ccgtcaccgg ccgggcggcg ctcgccggac ttccagtcac ccgcaagtcg
      241 gatctggttg cgctgcaaaa agccgaacgt ccattcggcg ggctgaacgc gactcccgtc
      301 tcgcagctcg gcaggatttt cgtttcaccc ggaccgatct acgaccccga gggacgcggc
      361 cgcgactggt ggcgtgccgg acgcgcgctt ttcgcggcgg gttttcgtgc cggcgatctg
      421 gccttgaatg ccttcgccta ccatttcacg ccggcgggct cgatgctcga atccggcgcg
      481 ctcgcgctcg gctgcgtcgt ggtgccgagc ggaaccggtc agaccgaaat gcaggtcgcg
      541 acgatcgccg atctgcgcgc gagcgcctac atggggacgc cctcgtttct caagctgatc
      601 gccgagaagg ctgacgagct gaaggcggac atcggcagcc tgaagaaggc ggcggtcagc
      661 gcggaatacc tgccgcccgc gctgcgcgcc gcgatgggcg agcgcggcat ccgcgtgctg
      721 cagtcctacg cttctgccga cctcgggctg atcgcctacg aatcggaggc gctcgaaggc
      781 atgatcctcg acgaagggct gatcctcgaa atcgtgcgcc cggggacggg cgacccgctg
      841 ccggagggcg aagtcgtcga ggtggtcgtc acgacgttca acccggacta cccgctcgtc
      901 cgcttcggca cgggagacct atccgccgta ttgcccggtg cgagtccttg cggccgcacc
      961 aacacgcgca tccgcggctg gatggggcgc gccgaccaga ccaccaaggt gaagggcatg
     1021 ttcgtccatc cctcgcaggt ggccgagatt ttgaagcgct atcccgaggt gctgaaggcg
     1081 cggctcgtcg tcgacaatcc cggaggcaac gaccgcatga ccttgcgctg cgaagtgcgg
     1141 agcgcggacg ccggcctcgc cgagaaaatt gctgcgagcc ttcgtgagat caccaagctg
     1201 cgcggcgagg tgcagttcgc gaagctcggc gagctggcga acgacggcaa ggtgatcgag
     1261 gacgcgaaaa aatatggcta ggcccgggcg gctctgcatc ctctggctcg cgacactgct
     1321 cgcgccgctt ggcgccggcg cggaagagat cgtcacggtc tctggccggc ccggcgcgac
     1381 gcagtcgtat ctgctgctgc actatgcgtc gccgcctgag gcggtggcgg tgctctttcc
     1441 gggcggcgaa gggctcctca gcgtgcgcgt cgagggcgat gccgtcaagt tccgggggcg
     1501 cgaaaatttt ctggtgcgca cgcgcacgct tctgcgcgac aagcaggtgg ccgtggccgt
     1561 cgtcgattcg ccttcggatc gccagcgcgc ggggatggac gacggctttc gcaccggccg
     1621 cgagcatgtc gaggacatcg ccgcagtggt caaggacctg aagcagcgct ttccgcaggc
     1681 gaagatcttt ctcatcggca cgagccgcgg cacgctctcg gctgcttata tcgggcgcgc
     1741 gctgaacaat gcggtggacg gagtggtgct gacctccacg gtgttctacg gcggcagacg
     1801 actcggcggc atcggcctcg ctgggttcga cttcgccgcg atcagggcgc ggctgctctt
     1861 cgttcatcac caggacgacg cctgcagggc ctgtccctac catcccgccg agaatctcgg
     1921 catgacctat cccctgatca cggtgaaagg cggcaagccg gccgagtccg atccctgcga
     1981 gccgttcgcg gcgcacggct acttcggcaa ggaagccgag accgtggccg ctatcaagcg
     2041 ctggatgctg ggcgaggctt tcccgaggac ggtcgaatag gtggaagtcc tcctggcccg
     2101 gctaaactag gaggtactct tccgaatcat gcgcatgaac gtgatcaaca tccgcgaagc
     2161 gaagacgcat ctctcgcgcc tggttgaaag ggccgcacga ggaattgaaa ttgtcatcg
//
LOCUS       DASPXW010000309         4191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_970846, whole genome shotgun sequence.
ACCESSION   DASPXW010000309 DASPXW010000000
VERSION     DASPXW010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970846"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VEL04_07020"
     CDS             complement(<1..206)
                     /locus_tag="VEL04_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48155.1"
                     /translation="MASYTLEGDLGRLRIALPRAPRRADRLWQHTCCEVFIGRKGLPG
                     YHEFNLAPSGEWAAYAFKGYRNGIP"
     gene            complement(308..532)
                     /locus_tag="VEL04_07025"
     CDS             complement(308..532)
                     /locus_tag="VEL04_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48156.1"
                     /translation="MNPILEGALIGLALALVLVAAEYMLLNKAAQARAKALHRAPQLE
                     EIERRRIASMARFSVLLPFIFAFFFWLIWG"
     gene            605..1528
                     /locus_tag="VEL04_07030"
     CDS             605..1528
                     /locus_tag="VEL04_07030"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYT48157.1"
                     /translation="MQPSSPDRSARLAAFAALALANLFWAGNWVIGRALREAFDPITL
                     NFWRWLVAAVVLAPFALPELVGKHAVIRRHAGLLALLAVTGVVIFQSLVYLGLRTTTA
                     INAVLLNSSAPLFMLLCSWVIERERATRRQVAGMLVSLCGILVIVSHGEPQRLLQLEF
                     HAGDAWILLAMPAWGIYSVLLKRRPPELGGLGLLFVISVAGLALLVPAFALQTLHAPP
                     RWPTPGEAAGVLYVGLAASVGAFICWNRGVATVGANAAGFTLHLLPAFGTVLAMVFLG
                     EAFSAFQAAGIATILVGVVLATRAATAASLR"
     gene            complement(1515..1808)
                     /locus_tag="VEL04_07035"
     CDS             complement(1515..1808)
                     /locus_tag="VEL04_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48158.1"
                     /translation="MEFRVGDIIAKCSGCGGTLFEPLYRNASSGPHSDFCCASCKTQT
                     SYSELIRRIGKEAARRSRERLTGSRKRTPEATQAGGTSVVPALILRPQKVTAS"
     gene            1886..3523
                     /locus_tag="VEL04_07040"
     CDS             1886..3523
                     /locus_tag="VEL04_07040"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HYT48159.1"
                     /translation="MITGAQAIVDCLRREGIDHVFGIPGTMNLPILDVLRATPELRFI
                     LTRHEQGAAFMAYGFARAVNRPSVVTATEGPGVTNLATGLGAAFKGYVPVISISGAQE
                     LWVREKDASQDIDQVTLHRPITKWAYSIPSVTKVQEAVRRAFRVALAEPLGPVHLDIS
                     KDILLEKTEAEPIAPQAYRPSSLPSCSSAELDRAARLIGQAKKPLLLAGGGVLREDAL
                     AALQRLADGTGIPVATLQYHPDAFPTSHRLALGPLGRNGWSSANKAMPQADVVIACGA
                     HIDLFSTTFKYGIISREAKLIHHSTVATDIGVVFPVAQAVVGSTLSFIEGLAERLDKT
                     WNWIDVARARKDWDAEREKLLDYKVKPILPPVVAHAMRQALPADGVMVLDAGNAGKHM
                     RLFMDTHQPNTFMYISDWGSVGAGLPMAMGVKLARPSQPVMATVGDMGMMCNIGELET
                     AVRERIPVVCVVFNDRGLGNERAFQKELYGGRIFGVDYGDVDFAALAKVLGAHGERVE
                     EPSGVLPAIRRALDSGLPAVVDVIIDQDTLAAVVYKS"
     gene            complement(3520..>4191)
                     /locus_tag="VEL04_07045"
     CDS             complement(3520..>4191)
                     /locus_tag="VEL04_07045"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYT48160.1"
                     /translation="RFVAFASVALQYPDLAVEQLDYAVKKLGLRGAGLGGSVNGEELA
                     NPKFHPFWARAEELGVPVFIHPQGTAELKGRLQGNGGLENTIGNPLETTLALSHLIFE
                     GTLDRFPGLKICAAHGGGYLPSYAPRSDHICLTFPDRCAAVPLKKRPTEYLKQLYFDA
                     LVFTPEALRHLAAQVGASQLVMGTDYPFPWTRTSVDHILDTPGFSDAERRAMLGETAA
                     KLLGI"
BASE COUNT          695 a         1366 c         1399 g          731 t
ORIGIN      
        1 ggaatgccgt tacgatagcc cttgaacgcg tacgcggccc actcgccaga gggcgcgaga
       61 ttgaattcgt ggtacccggg cagacccttg cgaccgatga atacctcgca gcaggtgtgc
      121 tgccacagcc tgtcggcgcg acgcggcgca cgcggtagcg caatacgcag acggccaaga
      181 tccccctcga gcgtgtagct cgccaccagc tcttcccgtg ttctacgaac gcgcgccgcg
      241 acggcgcgaa gcgcatcact gcgggattcc gggtggcacg cgagcgtaac cgcgtgatcg
      301 ggcgcgctca gccccagata agccagaaga agaacgcgaa gatgaacggc agcaggacgg
      361 aaaatcgcgc catcgatgcg atgcgccggc gctcaatctc ctcgagctgc ggcgcacgat
      421 gcagcgcctt ggcgcgcgcc tgcgctgcct tgttgagcag catgtattcg gccgccacca
      481 gcacgagcgc cagggcgaga ccgatcaggg caccttccag gatcggattc atggagactt
      541 cctgatgtcc atcatcgttt cactctaccg tatcacggca aacgttgcag gctatgcttc
      601 gcggatgcag ccatcaagcc cggacaggtc cgcgcgcctc gccgccttcg ccgcgcttgc
      661 gctcgccaac ctgttctggg cgggaaactg ggtcatcggg cgcgccttgc gcgaggcgtt
      721 tgacccgatc acgctcaatt tctggcgttg gctggtcgct gcagtggttt tagcgccgtt
      781 tgcgctgccg gagcttgtcg gcaagcatgc cgtaatcagg cgccacgctg gcctgctcgc
      841 gctgctcgcg gtcaccggcg tggtcatctt ccagagcctg gtctatctgg gactgaggac
      901 cacgacggcg atcaatgcag tgctgctcaa ctcctcggcg ccgctcttca tgctgctgtg
      961 ctcctgggtg atcgagcgcg agcgcgcgac gcgtcgccag gtcgccggca tgctggtgtc
     1021 gctgtgcggc attctcgtca tcgtgtcgca cggcgagccg cagagattgc tccagctcga
     1081 gttccacgcc ggcgacgcct ggatcctgct cgcgatgccg gcgtggggaa tctattcggt
     1141 gctgctcaag cgccgtccgc cggagctcgg cggcctgggc ttgctgttcg tcatctcggt
     1201 cgccggcctc gcgctcctcg tgccggcgtt cgcgctgcag acgttgcatg cgccgccgcg
     1261 ctggccgacg ccgggcgagg ccgccggagt gctttatgtc gggcttgccg cctcagtcgg
     1321 cgccttcatt tgctggaacc gcggcgtcgc gaccgtcggc gcgaatgccg ccggcttcac
     1381 cctgcatctc ctgcccgcat tcggtaccgt gctggcgatg gttttcctcg gcgaagcgtt
     1441 cagcgccttt caggcagcgg gcatcgcgac gatcctggtc ggcgtcgtcc tggcgacgcg
     1501 cgccgccacg gcagctagct tgcggtgact ttctgggggc gaagtatcag ggccggcaca
     1561 acggaggtgc cgccggcctg cgtcgcctcg ggagtgcgct tgcgcgagcc ggtgaggcgt
     1621 tccctggagc gcctggccgc ttccttgcct attcgccgga tcagctcgga atagctggtc
     1681 tgcgtcttgc aagacgcgca gcagaagtcg gaatgcgggc cgctggatgc attccggtac
     1741 aaaggttcga acagggtgcc gccgcacccc gagcacttgg cgataatgtc tccgaccctg
     1801 aattccatcg tccgctcctg accgtctttt acgatccaaa gtagcaggat cgggcaggcg
     1861 aaaacaggaa ctactagagg tttccatgat caccggtgcg caagcgatcg tcgattgtct
     1921 ccgccgggag ggaatcgatc acgtgttcgg cattccgggc accatgaacc tgccgattct
     1981 cgacgtgctg cgcgccaccc cggagctccg cttcattctc acccgccacg agcagggcgc
     2041 agcgttcatg gcctacggct tcgcgcgcgc ggtgaaccgg ccgagcgtgg tgacggcgac
     2101 cgaagggccc ggcgtgacga atctggcaac cggcctgggc gccgccttca agggctatgt
     2161 gcctgtcatc agcatcagcg gcgcgcagga attgtgggtg cgggagaagg acgcgagcca
     2221 ggacatcgac caggtcacgc tgcatcgccc gatcaccaaa tgggcctact cgatcccgtc
     2281 ggtgaccaag gtgcaggaag ccgtgcggcg cgcgttccgg gtcgccctcg ccgagccgct
     2341 cggcccggtg cacctcgaca tctcgaagga catcctcttg gagaaaaccg aggccgagcc
     2401 gatcgcgccc caggcgtacc ggccaagctc gctgccgagc tgctcttcgg ccgagctcga
     2461 ccgggcggcc agacttatcg ggcaagcgaa aaagccgctt ctgctggccg gcggcggcgt
     2521 gctgcgcgag gacgcgcttg ccgccctgca acgcctggcc gacggcacag gaattccggt
     2581 ggccacgctc cagtaccacc cggatgcgtt cccgaccagc caccgcctcg cgctggggcc
     2641 cctcggccgc aatggctggt cgagcgcgaa taaagccatg ccgcaagccg acgtggtgat
     2701 cgcctgcggc gcgcacatcg acctgttctc caccaccttc aagtacggca tcatcagccg
     2761 cgaggcgaag ctgatccatc acagcacggt ggcgaccgac atcggcgtgg tgtttccggt
     2821 agcgcaggcg gtcgtcggat cgacgctgag cttcattgaa ggcctggcag agcgtctcga
     2881 caaaacatgg aattggatcg atgtcgcgag ggcgcgcaag gactgggatg ccgagcgcga
     2941 gaaattgctc gattacaagg taaaacctat cctgccgccg gtcgtggcgc atgcgatgcg
     3001 ccaggcgctg ccggcggacg gagtcatggt gctggatgcg ggtaacgccg ggaagcacat
     3061 gcgcctcttc atggacacgc accagccgaa caccttcatg tacatcagcg actggggatc
     3121 ggtcggcgcg ggattgccga tggcgatggg ggtcaagctc gcgcggccga gccagccggt
     3181 gatggcgacg gtcggcgaca tgggaatgat gtgcaacatc ggcgagctgg aaaccgcggt
     3241 acgcgagcgc atcccggtgg tgtgcgtcgt gttcaacgac cgcggcctgg gcaacgagcg
     3301 cgcgttccag aaggagctct acggcgggcg catcttcgga gtcgactacg gcgacgtcga
     3361 tttcgcggct ctggcgaagg tcttgggcgc ccatggcgag cgcgtcgagg aaccctcggg
     3421 cgtgctgccc gcaatccggc gggcgctcga cagcggcctg cccgcggtgg tcgacgtgat
     3481 catcgaccag gacacgctcg ccgcggtggt ctataaaagc tagatgccga gcagtttcgc
     3541 cgccgtctcc ccgagcatcg ctctgcgctc ggcatcgctg aatcccggcg tgtcgagaat
     3601 gtggtcgacc gaggtcctgg tccatggaaa cggatagtcg gtgcccatga cgagctggct
     3661 cgcgccgacc tgcgccgcga gatggcgcag tgcctcgggc gtgaacacca gcgcgtcgaa
     3721 gtagagctgc ttcagatatt ccgtcgggcg ctttttcagc ggcaccgcgg cgcagcgatc
     3781 ggggaaggtc aggcagatgt gatccgagcg cggcgcgtac gaaggcaggt agccgccgcc
     3841 atgcgctgcg cagatcttca gccccggaaa gcggtccagc gttccctcga agatcaggtg
     3901 tgaaagcgcg agtgtggtct cgagcgggtt gccgatcgtg ttctcgaggc cgccgttgcc
     3961 ctgcagcctg cccttcaact cggccgttcc ctgcggatgg atgaagaccg gcacgccgag
     4021 ctcctcagcc ctggcccaga acggatggaa cttcggattg gcgagctcct cgccgttgac
     4081 gctgccgcca aggcctgcgc cgcgcaagcc gagcttcttc accgcgtagt cgagctgctc
     4141 cacggcgagg tccggatact gcagcgcgac cgaggcgaag gcgacgaagc g
//
LOCUS       DASPXW010000310         4366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_595884, whole genome shotgun sequence.
ACCESSION   DASPXW010000310 DASPXW010000000
VERSION     DASPXW010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4366
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595884"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="VEL04_07050"
     CDS             complement(<1..593)
                     /locus_tag="VEL04_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HYT48161.1"
                     /translation="MKRRDFLRTSAAAAASAAFPWKESFAETGWRTFEVTTRVEIVKP
                     QGVSRVWLPLPLASDTDWQRSFGSSRSGNAPRAEIMRDGRYGAAMLYAEWPAGQPSPS
                     LEVTSKFATRDRAVDLPRPNPLVKLLAAADAAFFTAPTDLIPTDGIVRKTALGIVKGA
                     RSDVDKARAIYEWIVDNTFRDPKTRGCGVGDIKAMLET"
     gene            complement(627..827)
                     /locus_tag="VEL04_07055"
     CDS             complement(627..827)
                     /locus_tag="VEL04_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48162.1"
                     /translation="MFLLRRLTSRSSRSIAVMIAFAQSGGGGARGGRMGSPDDERGFH
                     ALPPLLPLLQFSRNTPKNHATT"
     gene            complement(921..1157)
                     /locus_tag="VEL04_07060"
     CDS             complement(921..1157)
                     /locus_tag="VEL04_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48163.1"
                     /translation="MLKKLLVTLLASAFALTAFAQAPKAEPATPAAPKAQTGVDTKKA
                     TPATPSPKSSQGVKKAKPKAKVKAKADEKKAATK"
     gene            complement(1243..2313)
                     /locus_tag="VEL04_07065"
     CDS             complement(1243..2313)
                     /locus_tag="VEL04_07065"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HYT48164.1"
                     /translation="MPERRRPIVGRYTAEELALAARNRGMPLEALRHDVTPAGLHYLL
                     AHFDIPAADEGRWRLEVGGSVAQPFSLGVEELKALPARTLRVTLECAGNGRGQLSPRY
                     PSVPWLEEGVSTAEWTGVPLARLLERARLRAGVKDIAFHGADAGFDLDVEHHYARSLA
                     PEEALRDDILVCYAMNGAPLPPQHGAPLRLVVPRWYGMTSVKWLRAIEALGRPFDGLQ
                     QARSYHFRSAAGDKGEPCRLMRVNSLLAPPGIPDFYTRRRVVVAGEVPVMGRAWSGEG
                     AIATVQLGVDGEWRNAELEAPRDPYAWQGWSAVWQASAGEHELSCRATDAEGNQQPLE
                     PTWDLSGFGNNSMHRVRVTVRQ"
     gene            2341..3006
                     /locus_tag="VEL04_07070"
     CDS             2341..3006
                     /locus_tag="VEL04_07070"
                     /inference="COORDINATES: protein motif:HMM:NF016627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003839 - gamma-glutamylcyclotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061928 - glutathione specific
                     gamma-glutamylcyclotransferase activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HYT48165.1"
                     /translation="MKLDEFSAQLPHGDLWVFGYGSLMWSPCFSYKAKAAGQAHGYHR
                     ALCILSTRYRGTQRRPGLVMGLCRGGSCWGMAFRIDAAEVLRTLRRLWNREMPRRVYR
                     PRLVPVRLRSGRTIRALAFLADPAHRSYVRELDLHGRARLVAQGIGIRGPCVDYIQNT
                     LDHMHEVGVRDPHLERILHAALALRGIDGGFHPLQGLAKPGALRSKVKPHKARIAKLK
                     SRR"
     gene            complement(2890..4032)
                     /locus_tag="VEL04_07075"
     CDS             complement(2890..4032)
                     /locus_tag="VEL04_07075"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HYT48166.1"
                     /translation="MTFDKDAPAFDTLVERASTWSTRWERYAGRDVIPLWVADSDFRA
                     PPSVLEALEKRVKHGVFGYTTAPQELREAIVERLARLYHWRIDPSWIAFVPGVVPGLH
                     LAARKLLPPDGHALIPTPVYQHFKRAVELAPRAYTELPLVLEGGRWVYDCDFFRKAIS
                     AKTKMFFLCNPQNPGGTVFTRDELERLAGLARGCVIVSDEIHCELVLERDKGHVPIAS
                     LAPEISRRTVTLMSPNKAFNFPAAGCAWAIIEDANLRRIFSADLNAHVLPSPSVFGYE
                     AALAAYRGGNPWLAAQIDYLRANRDFLIRNLALPMAQVEATYLAWIDCSGLKTKDACE
                     LFLQHGVALSPGLQFGDARFVRLNFGTQRARLREALERMKAAINTP"
     gene            complement(4094..>4366)
                     /locus_tag="VEL04_07080"
     CDS             complement(4094..>4366)
                     /locus_tag="VEL04_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48167.1"
                     /translation="KAWSLATIYKGMFEFMVLQCIAIALIIIFPAICTWFPEHLQTQS
                     RAVKTEEVDDSMNRLEDDPLKAIQPQNEEEEVRGSPQQDKRPNTKK"
BASE COUNT          738 a         1445 c         1407 g          776 t
ORIGIN      
        1 gtctcgagca tcgccttgat gtcaccgacc ccgcagcctc tcgtcttcgg atcgcggaag
       61 gtgttatcca ctatccactc gtagatggcg cgtgccttat ccacatcgct acgcgcgcct
      121 ttgacgatac cgagagcggt cttcctgaca atgccgtcgg tcgggatgag atcggtcggc
      181 gcggtgaaga aagccgcgtc ggcggcagcc agcagcttca caagcggatt cggccgcggc
      241 agatccaccg cgcggtcgcg cgtcgcgaac ttgctcgtca cctcgagcga cggcgagggc
      301 tgcccggcgg gccattccgc gtacagcatc gccgcgccgt accggccgtc gcgcatgatc
      361 tcggcgcgcg gcgcgttacc cgaccggctg ctgccaaagc tgcgttgcca atccgtgtcg
      421 gaagcgagcg gcagcggcag ccagactctc gagacgccct gcggcttgac gatctccacc
      481 cgcgtcgtca cttcgaaggt ccgccacccg gtctcggcga acgactcctt ccagggaaac
      541 gctgcgctcg cagcggcggc ggccgaggtt ctcaggaaat cgcgtctttt cattggggct
      601 cctttcgcca acaatttgca ggcggcctag gtggtcgcgt gatttttcgg ggtgttgcgg
      661 gagaactgca ggaggggcag gagcgggggc agggcgtgaa atcctcgctc gtcgtcgggg
      721 cttcccatgc gtccgccccg cgcgccgccg ccgccggact gcgcaaaggc gatcatcacg
      781 gcgatggaac gcgagcttct ggaggtcagc ctgcgcagca ggaacatgcg aacaggcaat
      841 ttactccgcg cgccgcagct aaacaagcac caaaaaaaag cccggccgga gccgggcttt
      901 gcagttgctt cgagaaaccc ttacttcgta gcggctttct tttcgtcggc cttggccttc
      961 accttggctt ttggcttggc cttctttacg ccttgggaag acttgggcga cggcgtcgcc
     1021 ggcgtggctt tcttggtatc gacaccggtc tgcgccttgg gcgccgccgg ggtcgccggc
     1081 tccgccttcg gtgcctgggc gaaagcggtg agcgcaaacg ccgaggcgag caacgtgaca
     1141 agcagtttct tcaacatgga aaatcctcca gtttttgaaa gtcgatccga ttcggacctg
     1201 agggatacaa cgcaccggcc acgggcacgt tgacgcgcgg ccttattgcc gcacggtcac
     1261 ccggactcga tgcatcgaat tgttgccaaa gcccgacaga tcccaggttg gctcgagcgg
     1321 ctgctggttg ccctcagcgt cggtcgcgcg gcaggaaagc tcgtgctcgc cggcgcttgc
     1381 ctgccagacc gcgctccagc cctgccaagc gtaaggatca cgcggcgctt cgagttctgc
     1441 gtttcgccat tcaccatcca caccgagctg cactgtggcg atcgcgcctt cacccgacca
     1501 tgcgcgtccc atgaccggca cctccccggc caccacgacg cggcgccggg tatagaagtc
     1561 gggaattccc ggcggcgcga gcagcgaatt gacgcgcatc aggcggcaag gctcgccttt
     1621 gtcgccggcg gcgctgcgaa aatggtagct gcgcgcctgc tgcaggccgt cgaaaggccg
     1681 cccgagcgcc tcgatcgcgc gcagccactt cacgctcgtc atgccgtacc agcgcggtac
     1741 gacgagtcgc aacggcgcgc cgtgctgcgg cggcagcggc gcgccgttca tcgcgtagca
     1801 gacgaggatg tcatcgcgca gcgcctcctc cggcgcaaga ctgcgtgcgt agtggtgctc
     1861 gacgtccaag tcgaagccgg cgtccgcgcc atggaaagcg atgtccttta cgcccgcgcg
     1921 cagccgcgcg cgctcgagga gccgcgcgag cggcacaccg gtccattcgg cggtggacac
     1981 gccctcctcg agccagggca ccgagggata gcgcggcgag agctggccgc ggccgttgcc
     2041 cgcgcactcc agcgtcacgc gcagcgtcct cgcaggcagt gccttcagct cctcgacgcc
     2101 gagcgaaaac ggttgcgcca ccgagccgcc gacttccagt ctccagcggc cctcatccgc
     2161 ggcgggaatg tcgaagtgcg cgagcaggta atgcaggccg gccggcgtga cgtcgtggcg
     2221 cagcgcttcg agcggcatgc cgcgattcct tgcggcgagc gcaagctctt ctgcggtgta
     2281 gcggccgacg atcgggcggc gacgctccgg catcgggcga ttctataatc ggcccgtcac
     2341 atgaagctcg atgaattcag cgcgcaactg ccgcatggag atctgtgggt attcggctac
     2401 ggctcgctca tgtggtcgcc ctgcttttcc tacaaggcca aggccgccgg gcaggcgcac
     2461 ggctatcacc gcgccttgtg catcctgtcg acgcgctacc gcggcacgca gcgccgtccc
     2521 ggcctggtga tggggctgtg ccgcggcggc tcctgctggg gcatggcgtt tcgcatcgat
     2581 gccgccgagg ttctgcgcac cctccggcgg ctgtggaacc gcgagatgcc gcggcgcgtg
     2641 taccggccgc gcctcgtccc ggtgcggctg cgcagcgggc gcacgatcag agcgctggcg
     2701 tttcttgccg acccggcgca tcgctcctat gtgcgcgagc tcgacctgca cggccgcgcg
     2761 cgactcgtcg cgcagggcat cggcatccgc gggccttgcg tggattacat ccagaacacg
     2821 ctcgaccaca tgcacgaagt cggcgtgcgc gacccgcacc tcgagcgcat cctgcacgcc
     2881 gcccttgctt tacggggtat tgatggcggc tttcatccgc tccagggcct cgcgaagcct
     2941 ggcgcgttgc gttccaaagt taagccgcac aaagcgcgca tcgccaaact gaagtcccgg
     3001 agatagcgcg accccgtgct gcaggaaaag ctcgcaggca tccttggttt tcaagcccga
     3061 gcagtcgatc cacgccagat aggtggcctc gacctgcgcc attggaagag caagatttct
     3121 tattaaaaaa tctcggttag cacgcaagta gtcgatttgc gccgccagcc aagggttacc
     3181 gccgcggtat gcggccagcg ccgcctcgta gccgaagacc gacggcgacg gcaacacgtg
     3241 cgcgttcaga tccgcggaga agattcttct caaatttgca tcttctatga tcgcccaggc
     3301 gcagcccgcc gccggaaagt tgaacgcctt gttcggcgac atcagcgtca ccgtgcggcg
     3361 cgagatctcg ggcgcaaggc tcgcgatcgg cacatggccc ttgtcccgct cgagcaccag
     3421 ctcgcagtgg atttcatcgg acacgatgac gcagccgcgc gcgagcccgg cgagccgctc
     3481 gagctcatcg cgcgtgaaca ccgtgccgcc ggggttttgc gggttgcaca ggaagaacat
     3541 cttcgttttt gccgaaattg ccttcctgaa aaaatcgcag tcatagaccc agcgcccgcc
     3601 ctcgagcacg agcggcagtt cggtgtaggc gcgcggcgcg agctcgaccg cgcgcttgaa
     3661 gtgctggtag accggcgtgg gaataagcgc gtggccatcg ggcggcagca gcttgcgcgc
     3721 cgcaagatgc agccccggga cgacgccagg cacaaacgcg atccacgatg gatcgatgcg
     3781 ccagtggtag agccgcgcca ggcgctcgac gatggcctcg cgcagctctt gcggggcggt
     3841 ggtgtagccg aaaacgccat gctttaccct cttttcaagg gcttcaagga cagacggtgg
     3901 agcgcgaaaa tcgctgtcgg cgacccacag cggaatcacg tcgcgcccgg catagcgctc
     3961 ccagcgcgtg ctccaggtgc tggcgcgctc cacgagggta tcgaatgcgg gcgcgtcttt
     4021 gtcgaaggtc accgggttct tatagcaaaa aaagccgggc gcgaagcccg gccgatgtgc
     4081 aagttgagag ccgctatttc ttcgtgttcg gccgtttgtc ttgttgtggg ctgcccctca
     4141 cctcctcctc ttcgttctgt ggctggatgg ccttcagcgg atcatcctcg aggcggttca
     4201 tgctgtcatc gacctcctcg gtcttgaccg cgcgcgactg ggtctgcaga tgctccggaa
     4261 accaggtgca gatcgcggga aagatgatga tcagcgcgat cgcaatgcac tgcaggacca
     4321 tgaactcgaa catgcccttg tagatggtcg cgagcgacca ggcctt
//
LOCUS       DASPXW010000311         2755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1854264, whole genome shotgun sequence.
ACCESSION   DASPXW010000311 DASPXW010000000
VERSION     DASPXW010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1854264"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VEL04_07085"
     CDS             complement(<1..529)
                     /locus_tag="VEL04_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48168.1"
                     /translation="MQIRNPPAFKHRHAVDWTRERIEKLSKQEVQQLGVNATNLGEST
                     IAELCTEVLKARPRAGGKRKAGMPARPAVRHLISRRRAFEARGVMLQDVGGSWSGVRK
                     SDGTVVITLWADGVDSSAGGCSYLLWAPNEGGKRPWSDKAAGKERLEHCRLALERGGN
                     AEGLLVHGESLEGFIP"
     gene            605..1090
                     /locus_tag="VEL04_07090"
     CDS             605..1090
                     /locus_tag="VEL04_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48169.1"
                     /translation="MGSPSRQRIASWLMAALAGAAQAQAPQPRVCPDPARPCAGFKAN
                     DLSFALPDDGKARAEARSAPFFAVILRTAERCKLTPAQAKEVQSLFPRNKVFYTRFEC
                     DDDPENNVSYTNASPKFGFLAVYAGEERAAAEEFLEQVNALGLFPGANLRRMQVLFVY
                     P"
     gene            complement(1099..1764)
                     /locus_tag="VEL04_07095"
     CDS             complement(1099..1764)
                     /locus_tag="VEL04_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48170.1"
                     /translation="MSRIFFTLLTALIVKPLAAASFDCGEARTRTEKAVCADAELSHL
                     DELLGRFYHGARQQLAENSACLETDQRRWLRAVRDACAGTTCLKKVYLERLAELVALQ
                     PGMNIPRNLELPVYPALLWAIAPLPELKNAPRVAPHPLQVEGKLDFGCPECGYFLRAD
                     GGKTYVLIHDLFLGGATATQLAVLRETSRDARLMARGFARTDDPRQAGFDNQHCVYLH
                     QAP"
     gene            complement(1761..>2755)
                     /gene="dinB"
                     /locus_tag="VEL04_07100"
     CDS             complement(1761..>2755)
                     /gene="dinB"
                     /locus_tag="VEL04_07100"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HYT48171.1"
                     /translation="LRGKPVIVGWPGERSVVCAASYEARKFGIHSAMPATRARRLCPE
                     AEFIHPNFDKYRTVSRQIRDVFERHTPLVEPLSLDEAYLDVTENLWGEPLAQTIAVRI
                     KRNIFENLGLTASAGVAPNKFLAKIASGWEKPDGLTVIAPERVEKFLQQLPVEALWGV
                     GPVTAKKLRAIGIERLVEIRAVDRNLLAKTVGSLADWLVTLSHGEDPRQVKPDRESKS
                     VSCETTYAEDLTDLGEMNQEIARLARELAAELESKGIGARTVTIKVRYADFTTVTRSH
                     TEDFFTCSKAEFVNRAQMLLERTDAAQRPIRLLGVGAHGLAREASLLPLWEERP"
BASE COUNT          402 a          916 c          970 g          467 t
ORIGIN      
        1 cgggaatgaa gccctcgagg ctctcgccgt ggaccagcag gccttcggcg ttgccgccgc
       61 gctcgagcgc gaggcggcaa tgctcgagcc gctccttgcc cgccgccttg tcagaccagg
      121 gacgcttgcc gccttcgttc ggcgcccaca gcaggtagct gcagccgccc gcgctcgaat
      181 cgacgccgtc ggcccagagg gtgatgacca ccgtgccgtc ggacttgcgc acgccgctcc
      241 agctgccgcc cacgtcctgc agcatgacgc cgcgcgcttc gaacgccctg cggcgcgaga
      301 tcagatggcg caccgccgga cgtgccggca tcccggcctt gcgcttgccg ccggcgcgcg
      361 gccgcgcctt cagcacctcg gtgcacagct cggcgatcgt gctctcgccc aggttcgtgg
      421 cgttgacgcc gagctgctgg acctcctgct tgctgagctt ctcgatgcgt tcccgggtcc
      481 aatcgactgc gtggcgatgc ttaaaggctg gtgggttgcg tatttgcatg atcgcggcct
      541 ctgccgtggg gtgcgaagca gaggcatggg taggctaact gcttcatcta tcatgataac
      601 acctatgggg agcccatcgc ggcagcgcat cgcttcctgg ctgatggcgg cgctcgccgg
      661 ggcggcgcag gcgcaggcgc cgcagccgcg ggtgtgcccg gaccccgcca ggccctgcgc
      721 cgggttcaag gccaacgacc tgtcgtttgc gctgccggat gacggaaaag cgcgcgccga
      781 ggcgaggtcg gcgccgttct tcgccgtgat cctcaggacc gccgagcgct gcaagctgac
      841 gccggcgcaa gcgaaggaag tgcagtcgct gttcccccgc aacaaggtgt tctacacgcg
      901 cttcgagtgc gacgacgacc ccgagaacaa cgtcagctac accaacgcca gcccgaagtt
      961 cggcttcctc gcggtgtacg ccggcgagga gcgcgcggcg gcggaagagt tcctcgagca
     1021 ggtgaacgcg ctcggcctgt ttcccggcgc gaacctgagg cgcatgcagg tgctgttcgt
     1081 ctatccctga cccgggactc agggcgcctg atggaggtat acgcagtgct ggttgtcgaa
     1141 gccggcctgg cgagggtcgt cggtgcgcgc gaagccgcgc gccatgaggc gcgcatcgcg
     1201 gctcgtctcg cgcagcaccg cgagctgcgt cgcagtggcg ccgccgagaa acaggtcgtg
     1261 gatcaggaca taggtcttgc cgccgtcggc gcgcaggaag tagccgcatt ccgggcagcc
     1321 gaagtcgagc ttgccttcga cctgcagggg atggggcgcg acgcgcggcg cgtttttcag
     1381 ctcgggcagc ggcgcgatcg cccagagcag cgcggggtac accggcagct cgagattgcg
     1441 cgggatgttc attcccggct gcagcgccac cagctcggcg aggcgctcga ggtagacctt
     1501 cttcaggcag gtcgtgccgg cgcaggcgtc gcgcaccgcg cgcagccagc ggcgctggtc
     1561 ggtctcgagg catgccgagt tctccgcgag ctgctgccgc gcgccgtggt agaagcgccc
     1621 gagcagctcg tccaggtgcg acagctcggc gtcggcgcag accgcctttt cggtgcgcgt
     1681 gcgcgcctcg ccgcagtcga agctcgccgc ggcgagcggt ttcacgatga gggccgtcaa
     1741 cagggtgaag aaaattctgc tcatggccgt tcttcccaca atggcagcag cgaagcctcg
     1801 cgcgccaggc cgtgcgcgcc gaccccgagc aggcggatcg ggcgttgcgc ggcgtcggtg
     1861 cgctcgagca gcatctgcgc gcgattgacg aattccgcct tcgagcaggt aaagaaatcc
     1921 tcggtgtggc tgcgcgtcac ggtggtgaag tcggcgtagc gcaccttgat ggtgacggtg
     1981 cgcgcgccga tgcccttgct ttcgagctcc gccgcgagct cgcgggcgag gcgggcgatc
     2041 tcctggttca tctcgcccag gtcggtgagg tcctcggcgt aggtggtctc gcaggagacc
     2101 gatttcgact cgcggtcggg cttcacctgc cgcgggtcct cgccgtgcga gagcgttacc
     2161 agccagtcgg cgaggctgcc tacggttttt gcaaggagat tgcggtcaac ggctctgatc
     2221 tcgaccagcc gctcgatgcc gatggcgcgc agcttcttcg ccgtgaccgg gccgacgccc
     2281 cacagcgcct cgaccggcag ctgctgcagg aatttctcga cgcgctcggg cgcgatcacg
     2341 gtgaggccgt cgggcttttc ccagccggag gcgatcttgg cgaggaactt gttcggtgcg
     2401 accccggccg aggccgtcag gcccaggttt tcgaaaatgt ttctttttat tcgaacggct
     2461 atggtctgtg cgagcggctc gccccacaga ttttccgtga catcgagata ggcctcgtcg
     2521 agcgagagcg gctcgaccag cggcgtgtgg cgctcgaaga cgtcgcggat ctgccgcgac
     2581 acggtgcggt acttgtcgaa gttcggatgg atgaactccg cctccgggca gaggcgccgc
     2641 gcgcgcgtgg ccggcatggc cgagtggatg ccgaacttgc gcgcctcgta ggaggccgcg
     2701 cagacgacgc tgcgctcgcc cggccagccg acgatgaccg gcttgccgcg cagcg
//
LOCUS       DASPXW010000312         3844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_844824, whole genome shotgun sequence.
ACCESSION   DASPXW010000312 DASPXW010000000
VERSION     DASPXW010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844824"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VEL04_07105"
     CDS             <1..656
                     /locus_tag="VEL04_07105"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HYT48172.1"
                     /translation="RPFKGASTYELIDDILKGSPPPPSELRSGMPAALDEVLARAMAK
                     SRDGRYASWEDFGAELAKLLELERGDSELSDAEKFAASRRLKFFARFSDVELWEAVRI
                     SHWRHHQAGEELIREGSSDAHLYILASGLLKVTQRGKLLNAVSAGECVGEMAYARRDG
                     QARSATVASVEPSWAMRLRVEDIDELPAGSRARFIEAFLAIMADRLAMLGNRLIARV"
     gene            659..1159
                     /locus_tag="VEL04_07110"
     CDS             659..1159
                     /locus_tag="VEL04_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48173.1"
                     /translation="MGFFSRLLLVSFLLPLPVLAAYDANGVALGAPERDILRHFPSAH
                     CKALEWQSRAADRRCDDSRITFAGIEARVTFYLRNDRVEAFDLRFDSVEADRVVKFLK
                     ERYGAPASETRDSPGDQGKTPRQVYKVLWEAKGERAVLIAQLDRRRGSLLVSRGNFEE
                     EIYRVR"
     gene            complement(1162..1584)
                     /locus_tag="VEL04_07115"
     CDS             complement(1162..1584)
                     /locus_tag="VEL04_07115"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HYT48174.1"
                     /translation="MALKVSRTEVWSVMIDDRPGGAAEKLEALATAGANLEMVFARRM
                     PEQPGRGILFAGPIKGKKVVAAAQAAGFAMSDSIHGVKVEGGDKPGLGAKITRALANA
                     GVSFRGMSAAAIGTKFVSSIAVDSADDAARAVAALKKL"
     gene            1641..2213
                     /locus_tag="VEL04_07120"
     CDS             1641..2213
                     /locus_tag="VEL04_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase"
                     /protein_id="HYT48175.1"
                     /translation="MDRSAERKREIQAAVADVRAIEAGEGVSRDSLESIKRRLTRLAA
                     RQDLFAAADYPPPAPGGKLKSCLYRVAEDADHRFALYVNSSLGGHKTPAHNHTTWAVI
                     VGISGEELNRFYDRAASGVREKGQFVVKQGTGVAFMPEDLHSIHIQAPLLNFHMYGLA
                     LDQLHGREFYKAEEGVWKVFPAHADIREAR"
     gene            2188..3729
                     /locus_tag="VEL04_07125"
     CDS             2188..3729
                     /locus_tag="VEL04_07125"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HYT48176.1"
                     /translation="MPISARLVSERLAAGGELALLDVREQGVHYRGHPFFACSAPLSR
                     LELLIADLVPRRTATVVVLDGGNDGLSEKSQTKLKALGYSDVSILEGGCAAWQAAGFE
                     LFSGVNVPSKAFGEFIEHHYRTPRIAPGELKRLQGIKQVLILDSRPYEEYHRMNIPGG
                     IDVPGAELAYRIHDLAPSPDTLVVVNCAGRTRSIIGCQSLRNAGIPNPVAALKDGTMG
                     WELAGFSCERGAARVAPPPSEEGRKKAYAAAERVARRFDVRFCVSEEMRSWRKDADRT
                     LYLLDVRTREEFERSHVAGSLHAPGGQLVQAADEYVGVRNARVVLVDPDLVRAIMTTS
                     WLNQMGWADVYVLEGAELTETGPRKPKIPGTPPWQTVSALPERAAILDLSTSLRFNQR
                     HIPGAWWGVRSRFDHVKQNLPAFDSLVLTSEDGVLAHLAAPEAQSLWPGAQVRVLEGG
                     NAAWHGPWEPGVERATTARDDVWYKPYDHASDFATHAREYLEWEVALLEQIKRDPTIR
                     FRAYT"
BASE COUNT          628 a         1334 c         1254 g          628 t
ORIGIN      
        1 agcggccctt caagggcgca agcacctacg agctgatcga cgacatcctc aagggcagcc
       61 cgccaccgcc ctcggagctg cgctccggca tgcccgcggc gctcgacgaa gtgctggcgc
      121 gcgcgatggc gaagtcgcgc gacggccgct acgccagctg ggaggatttc ggcgccgagc
      181 tcgccaagct gctcgagctc gagcgcggcg attcggagct gtcggacgcc gagaagttcg
      241 ccgcctcgcg gcggctgaaa ttcttcgcgc gcttcagcga cgtcgagctg tgggaggccg
      301 tgcgcatctc gcattggcgc caccaccagg ccggggagga gctgatccgc gaaggctcgt
      361 ccgacgcgca cctctacatc ctcgcctcgg gcctgctcaa ggtcacgcag cgcggcaagc
      421 tgctcaatgc ggtcagcgcg ggggaatgcg tcggcgagat ggcctacgcg cgccgcgacg
      481 gccaggcgcg cagcgccacc gtcgcttcgg tcgagccgag ctgggcgatg cgcctgcgcg
      541 tcgaggacat cgacgagctc cccgcgggca gccgcgcacg cttcatcgag gccttcctcg
      601 ccatcatggc ggatcgcctg gcgatgctcg gcaaccggct gatcgcccgg gtctgagaat
      661 ggggtttttc agcaggctcc tgctcgtttc atttttgctg cccctgccag ttctggctgc
      721 ttatgacgcc aacggcgtcg cgctcggcgc gcccgagcgc gacatcctgc ggcattttcc
      781 cagcgcgcac tgcaaggcgc tggaatggca aagccgcgcc gccgaccggc gctgcgacga
      841 ttcgcgcatc accttcgccg ggatcgaggc gagggtcacc ttctacctga ggaacgacag
      901 ggtcgaggct ttcgacctgc gcttcgacag cgtggaagcg gaccgcgtgg tgaaatttct
      961 caaggagcgc tacggcgcgc ccgcatccga gacgcgcgat tcccccggcg accagggcaa
     1021 gacgccgcgc caggtctaca aggtgctgtg ggaagcgaag ggcgagcgcg ccgtgctgat
     1081 tgcccagctc gacaggcggc gcggctcgct cctcgtctcg cgcggcaatt tcgaagaaga
     1141 gatctaccgc gtgcggtagg gctagagctt cttcagcgcg gcgaccgctc tcgccgcgtc
     1201 gtcagcgctg tccaccgcga tgctgctgac gaacttggtg ccgatggccg ctgcgctcat
     1261 gccgcggaag ctgacccccg cgttcgccag cgcgcgcgtg atcttcgcgc ccaggccggg
     1321 cttgtcgccg ccctccacct tgacgccgtg gatactgtcg gacatcgcga agccggctgc
     1381 ctgcgccgcc gccaccacct tctttccctt gatcgggccg gcgaacagga tgccgcggcc
     1441 cggctgctcg ggcatgcggc gcgcgaacac catttccagg ttggcgccag ccgttgccag
     1501 cgcctccagc ttctccgcgg cgccccccgg gcggtcgtcg atcatcacgc tccacacctc
     1561 ggtgcggctc actttcaaag ccatggctgt gccctcctca gggttgagta cggtgataat
     1621 ttactcccag ggaggccgcc atggaccgat ccgcagagag gaagcgtgag attcaagccg
     1681 ccgtggccga cgtacgggcg atcgaggcgg gcgagggtgt aagccgcgac agcctggaga
     1741 gcatcaagcg gcggctcacg cggctggccg cgcggcagga tctgttcgcg gcggcggact
     1801 atccgccgcc ggcccccggc ggcaagctca agtcctgcct ctaccgcgtc gccgaagatg
     1861 ccgatcaccg cttcgccctc tacgtcaatt cctcgctggg cgggcacaag acgccggccc
     1921 acaaccacac cacctgggca gtgatcgtcg gcatcagcgg cgaggagctc aaccgcttct
     1981 acgaccgggc ggccagcggc gtgcgcgaga aggggcaatt cgtcgtcaag cagggaaccg
     2041 gggtcgcctt catgcccgag gacttgcact cgatccacat ccaggcgccg ctgctcaact
     2101 tccacatgta cggcctggcg ctcgaccagc tgcacggccg cgagttctac aaggcggaag
     2161 agggcgtctg gaaagtgttc ccggcacatg ccgatatccg cgaggctcgt tagcgagcgg
     2221 ctcgcggccg gcggcgagct ggcgctgctc gatgtgcgcg agcagggcgt gcactaccgc
     2281 ggccatccgt tcttcgcctg ctcggcgccg ctttcgcgcc tcgagctcct gatcgccgac
     2341 ctggtgccgc gccgcacggc gacggttgtg gttctcgacg gcggcaatga cgggctttcc
     2401 gagaaatctc aaaccaaact gaaagcgctg ggatactctg acgtatccat cctggaaggc
     2461 ggctgcgccg catggcaggc cgcgggcttc gagctcttca gcggcgtcaa cgtaccctcc
     2521 aaggcgttcg gcgagttcat cgagcatcac taccgcacgc cgcgcatcgc gcccggcgag
     2581 ctgaagcgcc tccaggggat caagcaggtc ttgatcctcg actcgcgacc gtacgaggaa
     2641 taccaccgca tgaacatccc gggcggcatc gacgtgcccg gggccgagct cgcttaccgc
     2701 atccacgatc ttgcgccgag tccggacacg ctggtggtgg tgaactgcgc ggggcgcacg
     2761 cgcagcatca tcggctgcca gtcgctgagg aatgccggga tcccgaaccc ggtggcggcg
     2821 ctgaaggacg gcaccatggg ctgggagctc gccggctttt cgtgtgaaag aggcgctgct
     2881 cgcgtggcgc ctccgccatc cgaagagggc aggaagaagg cctacgcggc ggccgagcgc
     2941 gtggcgcgcc gctttgacgt gcgcttctgt gtttcggaag aaatgagatc ctggaggaaa
     3001 gacgccgacc gcaccctcta cctcctcgat gttcgcacgc gggaggaatt cgagcgctcg
     3061 cacgtcgccg gctcgctcca tgcccccggc ggccagctgg tgcaggcagc cgacgagtac
     3121 gtcggcgtgc gcaacgctcg tgtcgtgctg gtggacccgg atctggtgcg cgcgatcatg
     3181 acgacgtcgt ggctgaacca gatgggctgg gccgacgtct acgtcctcga gggtgccgag
     3241 ctgaccgaaa ccggcccgcg caagccgaag atccccggaa ctcctccctg gcagacggtt
     3301 tccgcgctac ccgagcgcgc cgcgatcctc gacctctcta cgagcctgcg cttcaaccag
     3361 cgccacatcc cgggcgcgtg gtggggggtc cgctcgcgct tcgaccatgt aaaacagaat
     3421 ctgcccgcct ttgattctct agttcttact tccgaagacg gcgtgctggc gcacctcgcc
     3481 gcacccgaag ctcagagcct atggcccggc gcccaagtgc gcgtcctcga aggcggcaac
     3541 gccgcctggc acggtccatg ggaaccaggc gtcgagcgtg ccaccaccgc acgcgacgac
     3601 gtctggtaca agccctacga tcacgcttcc gacttcgcga cgcacgcgcg cgaatacctc
     3661 gaatgggaag tcgcgctcct cgagcagatc aagcgcgacc cgacgatccg cttccgcgct
     3721 tacacctaga cgagtagtcg gcgatgcttg cttaagccgt gcgactaccc ttcctcgtta
     3781 ccgacagtcc cccgaatagt cggaattgct tccgcctact gagcggcgta acttgctggc
     3841 ccaa
//
LOCUS       DASPXW010000313         5258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_971813, whole genome shotgun sequence.
ACCESSION   DASPXW010000313 DASPXW010000000
VERSION     DASPXW010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971813"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="VEL04_07130"
     CDS             <1..617
                     /locus_tag="VEL04_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011354246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYT48177.1"
                     /translation="ELTQRHPERFIGCFVYNPRCGIDNGVAELERHVKEHGFKMVQLH
                     ANMHAYRPDRAKDWIRPALKKCADLGLLVKLHTGDGPYSIPTEWVPIIKEFPSVDFIM
                     AHFGVQTGGVYCFEPFQWAMELDNVYCESGWCLQSRIVEFAKDLPKHKILFGSDTPPN
                     EPGMWIRLLEVLCMEPPQGMNLDEATLEDYLGNNVARMIGLKGN"
     gene            623..1537
                     /locus_tag="VEL04_07135"
     CDS             623..1537
                     /locus_tag="VEL04_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010812274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYT48178.1"
                     /translation="MIIDTHLHPTNVVDEAWRHTGTPFNGERMLKLMDGPYMINGKPR
                     RIDMGFVQPPPGNTVYRDGMRTGRQGIRDYMAYCAELTQKYPDRFIGNFSFNPRWGPE
                     EGAAELEFHVKEYGFKMLKLHSNMHNYRPDRALDWLRPAMRKCAELGVVVLIHTGDGP
                     YSIPTQFYPVIREFPMVNFIIGHFGIQTGGNYSFEAFWMAMDTRNIVCESGWTFQSRI
                     VEFAKDLPRHKIVFGTDSPPNEPGMWLRELEVLCSPPPQGMNLDEDGLEDYLGNNIAR
                     LCGIKTTPPPRSLDEAKARLKDTYAGVK"
     gene            1546..2532
                     /locus_tag="VEL04_07140"
     CDS             1546..2532
                     /locus_tag="VEL04_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYT48179.1"
                     /translation="MIAALTFGIAIQAKAQTNVRFLHEWRFEGHVAPFLVALDKGYYQ
                     AEGLNVTIDAGTGSLDSINRVATHAYDMSVADINSLMRYRDNPANAPLKAVMMVYDSP
                     PFAILTLKKNNIRTPKDLEGKVLGAPAADGAWAQWPILARVNNIDTAKVRVENVGFPV
                     REALLAQGRVDAVTAFGSNALGLVAQGIPESEIVVLMMRHFGLDLYGSAILAHPEFIK
                     TQPKAVAGLVRALIKGFQDTLRDPDTAIESVMKRNPVAKRDIELARLKKVIEQSYLTD
                     EVRANGFGAVDMKRLENAIDQVAIAYKFTHKPKAADIFTAEFLPPRGERLVK"
     gene            2529..3866
                     /locus_tag="VEL04_07145"
     CDS             2529..3866
                     /locus_tag="VEL04_07145"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HYT48180.1"
                     /translation="MKPDFQSFLDDYRSRYPDDVFHVTEEVSADQDVTAVVWTLAAQG
                     RDPMLVFERVNGTKVVTNIFASRKRIARLLGCDVSRIQQTYQEKSRKAVDPRVVPKGP
                     VLDSVEEQVDLTKLPLLRHFETDKAPYITNGIIVAEGPGNLSYHRAMLHSKTALATSL
                     HSRGHLWRLLQLAQEKNKALPVAMVIGAHPLFMLAASARVGFGADERRIAGGLLGEPL
                     EIVKTPKYQIGVPAAAEIVLEGTLDPGAKVEEGPFGEFTGYSSSRTTNTLFRVETLLR
                     RKDAMLVDVVGGNSPEHLNLSRVPRESEMAERLIERFPGVTRLHYPNSGVHFHAYVAL
                     RQRREGEARQVMLALLGWDPYLKTVIALDEDIDLDDDSQVMWALATHFQPQRDLLIVD
                     GLPGSALDPSASASGTTSRMGLDATRGPNFESVRAKISDRALARAKQLLQSVR"
     gene            complement(3828..4775)
                     /locus_tag="VEL04_07150"
     CDS             complement(3828..4775)
                     /locus_tag="VEL04_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HYT48181.1"
                     /translation="MNAIPVTILTGFLGSGKTTLLNYILTQKHGQRIAVIENEFGEIN
                     IDAELVMGSDEEIFELTNGCVCCVATARADLLRILEKLLARRAPLDHILVETSGLADP
                     TPVAQTLFANEKLMRETSLDAIVTLVDAKHAAAHLDNIAVDQIVIADRVIMNKVDLVG
                     ETELRDLEARVRRLNATAPILRCSHARVDLENILGIGAFSAQRRMVSLDEHEHSHDPA
                     LESESFVFERPFDRSRLELYFKQLLRDKGDNIFRLKGILALDGEPRRYVLQCVHRIMD
                     LKPADAWGEERPVSKLVFIGRHLERAALANGLEQLLRPG"
     gene            complement(4772..>5258)
                     /locus_tag="VEL04_07155"
     CDS             complement(4772..>5258)
                     /locus_tag="VEL04_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HYT48182.1"
                     /translation="FVASSTYYPTKSYSFAAHFVEVSVDAETGQVDVVQVIPVHEIGR
                     VIHPVGAAGQIEGGIQQGIGHTLTEEHVVDLATGRSLNPSFVDYKMPLAMDMPPIRTI
                     LLETAPDPGGPFGAKGVGEDPIIAIGPAIANAIYAAIGVRFRHYPITPEQVLQALREK
                     S"
BASE COUNT         1001 a         1691 c         1662 g          904 t
ORIGIN      
        1 tcgagctgac gcagcgccat cccgagcgct tcatcggctg cttcgtctac aacccgcgct
       61 gcggcatcga caacggcgtc gccgagctcg agcgccacgt caaggagcac ggcttcaaga
      121 tggtgcagct gcacgccaac atgcacgcct acaggcccga ccgcgccaag gactggatcc
      181 ggccggcgct gaaaaaatgc gccgacctcg gcctgctggt gaagctgcac accggcgacg
      241 gcccgtacag catcccgacc gagtgggtgc cgatcatcaa ggaattcccg tccgtcgact
      301 tcatcatggc gcacttcggc gtgcaaaccg gcggcgtcta ctgtttcgag ccgttccagt
      361 gggcgatgga gctcgacaac gtctactgcg agtcgggctg gtgcctgcag tcgcgcatcg
      421 tcgagttcgc gaaggacctg ccgaagcaca agattctctt cggctcggac acgccgccga
      481 acgagccggg gatgtggatc cgcctgctcg aagtgctgtg catggagccg ccgcagggca
      541 tgaacctcga cgaggccacg ctggaggatt acctgggcaa caacgttgcc cgcatgatcg
      601 gattgaaggg aaactgatcc gcatgatcat tgatacccat cttcacccga ccaacgtcgt
      661 cgacgaagcc tggcggcaca ccggcacgcc gttcaacggc gaacgcatgc tcaagctgat
      721 ggacggccct tacatgatca acggcaagcc gcggcgcatc gacatgggct tcgtccagcc
      781 gccgcccggc aacaccgtct accgcgacgg catgcgcacc ggccgccagg gaatccgcga
      841 ctacatggcg tattgcgccg agctgacgca gaaatacccg gaccgcttca tcggcaactt
      901 cagcttcaac ccgcgctggg gtcccgaaga gggcgccgcc gagctcgagt tccacgtcaa
      961 ggagtacggc ttcaagatgc tgaagctgca ctccaatatg cataactaca ggccggatcg
     1021 cgcgctcgac tggctgcggc ccgcgatgag gaaatgcgcc gagcttggcg tcgtcgtact
     1081 gatccatacc ggcgacgggc cgtacagcat cccgacgcag ttctacccgg tgatccgcga
     1141 attcccgatg gtgaacttca tcatcgggca cttcggcatc cagaccggtg gcaactactc
     1201 gttcgaggcc ttctggatgg cgatggacac cagaaatatc gtgtgcgagt cgggctggac
     1261 cttccagtcg cgcatcgtcg agttcgcgaa ggatctgccg cggcacaaga tcgtgttcgg
     1321 caccgactcg ccgcccaacg agcccggcat gtggctgcgc gaactggagg tgctgtgctc
     1381 gccgccgccg cagggcatga acctcgacga ggacgggctc gaggactacc tcggcaacaa
     1441 tatcgcgcgc ttgtgcggca tcaagaccac gccgccgccg cgaagcctcg atgaagccaa
     1501 ggcgcggctc aaggacacgt acgcgggggt caaatgattc gaatcttgat tgcagccctc
     1561 acttttggga ttgcgattca ggcaaaagcc caaaccaacg tgcgcttcct gcacgagtgg
     1621 cgcttcgaag gacatgtcgc tcccttcctc gtcgcgctcg acaagggcta ctaccaggct
     1681 gagggcctga acgtcaccat cgacgcgggg accggatcgc tcgacagcat caaccgcgtc
     1741 gccacgcatg cctacgacat gagcgtcgcc gacatcaatt cgctcatgcg ctaccgcgac
     1801 aacccggcca acgcgccgct caaggcggtg atgatggtct acgattcgcc gccgttcgcc
     1861 atcctgacac tgaagaaaaa caacatccgc acgccgaagg acctcgaggg caaagtgctc
     1921 ggcgcgccgg cggccgacgg cgcctgggcg cagtggccga ttctcgccag ggtgaacaac
     1981 atcgacacag cgaaggtacg cgtcgagaac gtggggttcc cggtgcgcga ggcgctactc
     2041 gcacagggca gggtggacgc ggtgacggca ttcggcagca acgcgctcgg ccttgtcgcc
     2101 cagggtattc cggagagcga gatcgtcgtg ctgatgatgc ggcacttcgg cctcgacctg
     2161 tacggcagcg cgatcctcgc gcatcccgag ttcatcaaaa cgcagccgaa agccgtcgcg
     2221 ggcttggtgc gcgcgctgat caagggcttc caggacacgt tgcgcgatcc cgatacggca
     2281 atcgagagcg tgatgaagcg caacccggtg gccaagcgcg acatcgagct cgcgcggctg
     2341 aagaaggtga tcgagcagag ctacctcacc gacgaggtgc gcgccaacgg cttcggcgct
     2401 gtcgacatga agcgcctgga aaacgcgatc gaccaggtcg cgatcgccta caagttcacc
     2461 cataagccca aggccgccga catcttcacc gccgagtttc tgccgccgcg cggcgagcgg
     2521 ctggtgaagt gaagccggat ttccaaagct tcctggatga ctaccgtagc cggtatcccg
     2581 atgatgtatt tcatgtaaca gaagaagtct ccgccgacca ggacgtcacc gcggtcgtct
     2641 ggacgctcgc cgcgcagggc cgcgacccga tgctggtgtt cgagcgggtg aacggaacga
     2701 aggtcgtcac caacatcttt gcctcgcgaa agcgcatcgc gcggctgctt ggttgtgacg
     2761 tttcgcgtat tcaacagacc taccaggaga aatcccgcaa ggcagtcgat cccagggtgg
     2821 tgccaaaagg cccggtgctc gattcggtcg aagagcaagt cgatctgacg aagctgccgc
     2881 tgctcaggca tttcgagacg gacaaggcgc cctacatcac caacggcatc atcgtcgccg
     2941 aaggccccgg aaatctctcg tatcaccgcg cgatgctgca ctcgaagacc gcgctcgcca
     3001 ccagcctgca ttcgcgcggc cacttgtggc gcctgctgca actcgcacag gaaaaaaata
     3061 aagcgctccc ggttgccatg gtgataggag cacaccccct gttcatgttg gcggcgtccg
     3121 cgcgcgtggg gttcggcgcg gatgaacggc gcattgccgg cggtctgctt ggcgaacctc
     3181 tggagatcgt caaaaccccc aaatatcaga tcggcgtgcc ggccgctgcc gagatcgtcc
     3241 tcgagggaac gctcgacccc ggggcgaagg tcgaggaagg accgttcggc gaattcaccg
     3301 gctactcgtc cagccgcacc accaacaccc tgtttcgcgt cgagacgctg ctgcgccgca
     3361 aggatgcgat gctggtcgac gtggtcggcg gcaactcgcc cgagcatctg aatctctctc
     3421 gcgtgccgcg cgagtccgag atggcggagc ggctgataga gcgctttccg ggcgtgacgc
     3481 ggctgcacta cccgaattcc ggcgtgcact tccacgccta cgttgccttg agacagcgcc
     3541 gcgagggaga ggcgcggcaa gtcatgctcg ccctgctcgg ctgggaccct tacctgaaga
     3601 cggtgatcgc gctcgacgag gacatcgacc tcgacgacga ctcgcaggtg atgtgggcgc
     3661 tcgcaacgca cttccagccg cagcgcgatc tgctgatagt cgacgggctg cctggaagcg
     3721 cgctcgatcc gtcggcctcc gcctcgggca ccacctcgcg catggggctc gatgccacgc
     3781 gcggtccgaa tttcgaaagc gtgcgcgcga agatttccga ccgggcgcta gcccgggcga
     3841 agcagctgct ccagtccgtt cgctagcgcg gcgcgctcga ggtggcggcc gatgaacacc
     3901 agcttgctca ccggcctttc ctcaccccac gcgtcggcgg gcttcaggtc catgatgcgg
     3961 tgcacgcact gcagcacata gcggcgcggc tcgccatcga gcgcgaggat gcctttcagg
     4021 cggaagatgt tgtcgccctt gtccctcagt agttgcttga aatagagctc caagcgcgag
     4081 cggtcgaatg gccgctcgaa gacgaatgac tccgactcga gagccgggtc gtggctgtgc
     4141 tcgtgctcgt ccagcgaaac catgcggcgc tgcgcgctga aagcgccgat gccgaggatg
     4201 ttctcgagat caaccctggc atggctgcaa cgcaggatcg gcgccgtggc gttgaggcgc
     4261 cgcacccgcg cctcgagatc gcgcaactcg gtctcgccga ccagatcaac cttgttcatg
     4321 atgacgcgat ccgcgatgac gatctgatcg acggcaatgt tgtcgaggtg cgcggctgca
     4381 tgctttgcat cgaccagggt cacgatcgcg tcgagcgacg tttcgcgcat gagcttctcg
     4441 tttgcaaaga gtgtctgcgc gaccggtgtc ggatcggcaa gaccgctggt ttcgacgagg
     4501 atatggtcga gcggcgcgcg tcgtgccagc agcttctcca ggatgcgcag caggtcggcg
     4561 cgcgccgtgg cgacgcagca cacgcagccg ttggtgagct cgaagatctc ctcgtccgag
     4621 cccatcacga gctcggcatc gatgttgatc tcgccgaact cgttctcgat caccgcgatg
     4681 cgctgcccgt gcttctgcgt caggatgtag ttcagcagcg tggttttccc cgagccgaga
     4741 aaaccggtga ggatggtcac gggaatggcg ttcaagattt ttccctcaag gcctgcagaa
     4801 cctgctcagg ggtgatcggg taatggcgga agcgcacgcc gatcgcggcg tagatcgcgt
     4861 tcgcgatcgc cgggccgatg gcgatgatcg ggtcctcgcc cacgcccttg gcgccgaacg
     4921 gcccgccggg atcgggcgcg gtctcgagca ggatggtgcg gatgggcggc atgtccatgg
     4981 cgagcggcat cttgtagtcg acgaagcttg ggttgagcga gcgcccggtg gcgaggtcga
     5041 cgacgtgctc ctcggtgagc gtgtgcccga taccctgctg gatgccgccc tcgatctgcc
     5101 ctgccgcgcc gaccgggtgg atcactcttc cgatctcgtg caccgggatc acctgcacga
     5161 cgtcgacctg cccggtctcg gcatcgacgc tgacctcgac gaagtgcgct gcgaacgagt
     5221 acgacttggt cggatagtaa gtgctgctcg ccacgaaa
//
LOCUS       DASPXW010000314         2550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1478594, whole genome shotgun sequence.
ACCESSION   DASPXW010000314 DASPXW010000000
VERSION     DASPXW010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478594"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /gene="hemC"
                     /locus_tag="VEL04_07160"
     CDS             <1..738
                     /gene="hemC"
                     /locus_tag="VEL04_07160"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HYT48183.1"
                     /translation="FVMELESAMAESRADLAVHSMKDVPAELPPEYVLAAITAREDPR
                     DAFVSNRFHSFAEMPAGAVVGTSSLRRSAQVAERHPGLQIKLLRGNVETRLAKLDRGE
                     YDAVVLAVAGLARLGLEARIASRLELEESLPAPGQGALGVECLASDKSMVTLLAPLDD
                     AVTARCVRAERAVSRALGGSCALPLGAYAELAGARLRLRALVASADGARVIRAQREGE
                     DPERLGAQVAEELRAQGAQEILSSIEK"
     gene            728..1483
                     /locus_tag="VEL04_07165"
     CDS             728..1483
                     /locus_tag="VEL04_07165"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HYT48184.1"
                     /translation="MKNNRLAGRGILVTRPRALAAGLATLIEQSGGRAVLFPAIEIED
                     LAPPAALQDLDAYDLAVFISPTAVQKVMERVAAWPKRLRTAAVGSGTRRELERRGVSA
                     VIAPESAADSEALLALAELERVAGQRVVIFRGGPGRSLLGDTLAARGAQVQYAECYRR
                     TRPRSDPAPLLEAWARGAVHAVTVSSSEGIENLFALLGAPGEQRLRSTPLFVPHPRVG
                     AHARQRGVREVVVGGAADDAMIERLVAYFNELA"
     gene            1470..2510
                     /locus_tag="VEL04_07170"
     CDS             1470..2510
                     /locus_tag="VEL04_07170"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF016275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HYT48185.1"
                     /translation="MNSPEPAAAAPAPAARGSRAPRLALIACLFAAALAALFWIDARN
                     RIGATQEEIARRLRDIEADSREARAVARQAQEALRETQAKLGQLEARFAESQSHQLAL
                     EALYQELSRNRDAWQLAEIEQVLAIASQQLQLAGNVRAALLALQLAEARLARADRSQF
                     APLRRALARDIERLRSAPAVDLPAMSMRLDSLVAAVDALPLAFDERAERVPVKEAQPG
                     GERGVVARLGAEVWNELRQLVVVRRVDAPEPPLLPPSQAYFLRENLRLRLLNARLSLL
                     ARDEAGYREDLRAAQAWIRRYFDARSKLAIDALTQIKQLSATSISFEMPSISESLDAV
                     RGFKSRRERAPG"
BASE COUNT          332 a          904 c          928 g          386 t
ORIGIN      
        1 ttcgtgatgg agctggagag cgcgatggcg gaatcgcgcg ccgacctcgc ggtgcattcg
       61 atgaaggacg tgccggcgga attgccgccg gagtacgtgc tcgccgccat caccgcgcgg
      121 gaggatccgc gcgatgcttt cgtttcgaat cgctttcatt cttttgcgga aatgcctgcc
      181 ggcgccgtgg tcggcacctc cagcctgcgg cgctcggcgc aggtcgccga gcgccatccc
      241 gggctgcaga tcaagctgtt gcgcggcaac gtggaaaccc gcctcgcaaa gctcgaccgc
      301 ggcgaatacg acgcggtcgt gctcgccgtg gcgggccttg cgcggctcgg cctcgaggcg
      361 cgcatcgcct cgcgcctgga gctcgaagag agccttcccg cgccgggcca gggggcgctc
      421 ggcgtcgaat gtctggcgtc tgacaaaagc atggtcacgc tcctggcgcc gctcgatgac
      481 gctgtgaccg cgcggtgcgt gcgtgccgag cgggcggtga gccgggcgct cggcggtagc
      541 tgtgccctgc cgctcggcgc ttacgccgag ctcgccgggg ctcggctgcg gctgcgcgcg
      601 ctcgtcgcct cggccgacgg cgcgcgcgtg atccgtgcgc agcgcgaagg cgaagacccc
      661 gagcgcctcg gcgcacaggt tgccgaggag ctgcgcgcgc aaggcgccca ggaaattctt
      721 tcctccattg aaaaataatc gcctcgctgg ccgcggcatc ctcgtgaccc gcccccgcgc
      781 gctcgccgcc ggcctggcga cgctgatcga gcagtcaggc gggcgcgcgg tcctattccc
      841 cgcgatcgag atcgaggacc tggcgccgcc ggcggctttg caggatctag acgcctacga
      901 cctcgcggta ttcatcagcc cgaccgcggt gcagaaggtg atggagcgcg tcgcggcgtg
      961 gccgaagcgc ctgcgcaccg ccgcggttgg ctcgggaacg cgccgcgagc tcgagcggcg
     1021 cggcgttagc gcggttattg cacccgagtc cgccgccgac agcgaggcgc tgctcgcgct
     1081 cgctgagctc gagcgcgtcg ccgggcagcg cgtggtcatt tttcgcggtg ggcccggacg
     1141 cagcctcctc ggcgatacgc tcgcggcgcg cggcgcgcaa gtgcagtacg ctgagtgcta
     1201 ccggcgcacg cggccacgaa gcgaccccgc gccgctgctc gaggcctggg cgcgcggcgc
     1261 cgtgcatgcg gtcaccgtgt cttcgtccga gggcatcgag aatctcttcg cgctgctcgg
     1321 cgcgccgggc gagcagcggc tgcgctcgac tcccctgttc gttccccacc cccgggttgg
     1381 tgcgcacgcg agacagcgcg gcgtgcgcga ggtcgtcgtc ggcggcgccg cagacgacgc
     1441 gatgatcgag cggctggtag catatttcaa tgaactcgcc tgaaccggct gcagcggccc
     1501 cggcgcccgc ggcgcgcggc tcgcgcgcgc cgcggctcgc gctgatcgcg tgcctcttcg
     1561 ccgccgcgct tgccgcgctg ttctggatcg acgcgcgcaa tcgcatcggc gcgacccagg
     1621 aggagattgc gcgccgcctg cgcgacatcg aggccgactc gcgcgaggcg cgcgcggtgg
     1681 cgcgccaggc gcaggaggcg ctgcgcgaga cacaggcgaa actcgggcag cttgaggcgc
     1741 ggttcgccga gtcgcaaagc catcagctcg cgctggaggc cctgtaccag gagctttccc
     1801 gcaaccgcga cgcatggcag cttgccgaga tcgagcaagt gctcgcgatc gcttcacagc
     1861 agctgcagct cgcgggcaac gtgcgcgcgg cgctgctcgc gctgcagctc gccgaggcgc
     1921 gcctcgcgcg cgccgaccgc tcgcagttcg cgccgctgcg acgggcgctc gcgcgcgaca
     1981 tcgagcgcct gaggtcggca cccgctgtcg acctccccgc catgagcatg cgcctggaca
     2041 gcctggttgc cgcggtggat gctttgccgc tcgccttcga cgagcgtgcc gagcgcgtgc
     2101 cggtcaagga ggcgcagccg ggcggggagc gcggagtcgt ggcgcggctc ggcgccgagg
     2161 tctggaacga gctcaggcag ctggtggtgg tgcggcgcgt cgacgccccg gagccgccgc
     2221 tgctgccgcc gtcgcaggca tacttcctgc gcgaaaactt gcgcctgcgg ctcctcaacg
     2281 cgcggctttc gctcctcgcg cgcgacgagg cgggctaccg cgaggacctg cgggcggcgc
     2341 aggcctggat ccggcgctat ttcgacgcgc gctcgaagct cgccatcgac gcgctcacgc
     2401 agatcaagca gctttccgcg acttcgatca gcttcgagat gccgagcatc tccgaaagcc
     2461 tggatgcggt gcgcggattc aagtcgcggc gcgagcgcgc ccctggatga agtccctgct
     2521 ctggctgctg gcggtcttcg cggccgcagt
//
LOCUS       DASPXW010000315         2268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_343386, whole genome shotgun sequence.
ACCESSION   DASPXW010000315 DASPXW010000000
VERSION     DASPXW010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343386"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..1065)
                     /locus_tag="VEL04_07175"
     CDS             complement(58..1065)
                     /locus_tag="VEL04_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HYT48186.1"
                     /translation="MDSVDIEVLRTAEAWRSEGRQVALGTIVKTWGSAPRPVGAMVAI
                     RDDGQVVGSVSGGCVEDDLIEKIKNKLIAARKPELVTYGVTNEEATRWGLPCGGTLQL
                     VLEPLSGKSGVAELLERIGQQQLVKRRLEMDSGSATLAPGRWQDVLEFDGKVLSAVHG
                     PRWRLVLIGAGQLTRYLAEMAHMLDYHVTVIDPREEYAAGWDLAGVPLDRGMPDDVVR
                     ELNLDGHSAIVALTHDPKLDDLALMEALKSAAFYVGAIGSKKNNDARRERLREFDLSA
                     DEIARLRGPVGLYIGSKTPPEIAVAILAEMTAVRHGVAEPSWAAKPERRFDPSACEVK
                     T"
     gene            complement(1058..>2268)
                     /locus_tag="VEL04_07180"
     CDS             complement(1058..>2268)
                     /locus_tag="VEL04_07180"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HYT48187.1"
                     /translation="IRASEAGYRVDLPLAVEQVLRADRYDTIFYRVTAPDGREIAGES
                     DLPAPPHEAEAREGLVSYDTQYKGRKVHAVSMPAQCGRSTCTVLVAETTVKRSRAARD
                     ILLSNVLPEALIALATLLIVWFGVKRGLAPLARVSEEIKERSPNDLHALDAVRAPDET
                     RPLIAALNGLLEQLAAANRNQQRFLANAAHQLRTPLAGLQAHTELALSLPLPDACRAQ
                     VEQVHGATIRTARLANQLLVLARAEPGGHAEPRSRVDLKRVVEGAADEWVHRALERDI
                     DLGFDLEPAAVRGDEFLLREALANLLHNAIEYSQRGARVTVRTGLRAEQAFVEVEDEG
                     PGIPAAERERVLERFYRVPGTPGTGSGLGLAIVREIAAGHAASIAISEGAGAGNATKG
                     CRVALTFPHG"
BASE COUNT          316 a          801 c          792 g          359 t
ORIGIN      
        1 tcggagccga agcggctcgc cgagccggcg gcgagcagca gcccgacgat tttcaattca
       61 ggtcttgact tcgcaggccg aaggatcgaa gcggcgctcg ggcttcgcgg cccagctcgg
      121 ctcggcgacg ccgtggcgca ccgcggtcat ctcggcgagg atcgcgaccg cgatctcggg
      181 cggggtcttg gagccgatgt acagccccac cggcccgcgc aggcgggcga tctcgtccgc
      241 ggacaggtcg aactcgcgca gccgctcgcg acgcgcgtcg ttgtttttct tcgagccgat
      301 cgcgccgacg tagaacgccg ccgatttgag cgcctccatc agcgccaggt cgtcgagctt
      361 cgggtcgtgg gtgagcgcga cgatcgcgct gtggccgtcg aggttcagtt cgcgcaccac
      421 gtcgtcgggc atgccgcggt cgagcggcac gccggcgagg tcccagcccg ccgcgtactc
      481 ctcgcgcgga tcgatgaccg tgacgtggta atcgagcatg tgcgccatct ccgcgagata
      541 gcgcgtgagc tggccggcgc cgatcagcac caggcgccag cgcggaccgt gcaccgccga
      601 aagcaccttg ccatcgaact cgagcacgtc ctgccagcgc ccgggcgcga gcgtggcgct
      661 gccgctgtcc atctcgaggc gccgcttcac gagttgctgt tgtccgatgc gctccagcaa
      721 ttcggcgacg ccacttttcc ccgacaaagg ctcgaggacg agctgcaggg tgccgccgca
      781 cggcaggccc cagcgcgtcg cttcttcatt ggtaacgccg taggtaacga gttcgggctt
      841 gcgcgccgcg atcaacttgt tttttatttt ttctatcagg tcatcttcca cgcagccgcc
      901 cgagacggag ccgaccacct ggccgtcgtc gcggatcgcg accatcgcgc ccacgggtcg
      961 cggcgccgag ccccaggtct tgacaatggt gccgagcgcg acctgccggc cctcgctgcg
     1021 ccaggcctcg gccgtacgca gcacttcgat atcgacgcta tccatgggga aatgttagcg
     1081 caactctgca acccttggtt gcattgccgg cgcccgcgcc ttcgctgatc gcgatcgacg
     1141 cggcatggcc ggcggcgatc tcgcgcacga tcgcgagccc aaggccgctg ccggtgcccg
     1201 gcgtgcccgg cacccggtag aagcgctcga ggacccgctc gcgctcggcc gcggggatgc
     1261 ccgggccctc gtcctcgacc tcgacgaacg cctgttcggc gcgcagcccg gtgcgcaccg
     1321 tcacgcgggc accgcgctgc gagtactcga tcgcgttgtg cagcaggttg gcgagcgcct
     1381 cgcgcagcag gaactcgtcg ccgcgcaccg ctgccggctc gaggtcgaag ccgaggtcga
     1441 tgtcgcgctc gagcgcgcgg tgcacccatt cgtcggcggc gccttccacc acccgcttga
     1501 gatcgacgcg gcttcgcggc tcggcatgcc cgccgggctc ggcgcgcgcc agcaccagca
     1561 gctggttcgc gagccgcgcg gtgcggatcg tcgctccgtg cacctgctcc acctgcgcgc
     1621 ggcaggcgtc cggcagcggc agcgaaagcg cgagctcggt atgggcctgc aggccggcaa
     1681 gcggcgtgcg cagctggtgc gcggcgttgg cgagaaagcg ctgctggttg cggttcgccg
     1741 cggcgagctg ctcgagcagc ccgttcagcg cggcgatcaa ggggcgcgtc tcgtcgggcg
     1801 ccctgacggc atcgagggcg tgcaggtcgt tcggcgagcg ctccttgatc tcctcggaga
     1861 cgcgcgcgag cggcgccagg ccgcgtttca ccccgaacca cacgatgagc agcgtcgcca
     1921 gcgcgatcag cgcctcgggc agcacgttcg agagcaggat gtcgcgcgcc gcccgcgagc
     1981 gcttgaccgt cgtctcggcg accagcacgg tgcaggtgga gcggccgcac tgcgccggca
     2041 tcgaaaccgc gtgcaccttg cgacccttgt attgggtgtc ataggagacc aggccttcgc
     2101 gcgcttcggc ttcgtgcggc ggcgcgggca ggtccgattc gccggcgatt tccctgccgt
     2161 ccggggccgt gacccggtaa aaaatcgtgt cgtagcggtc cgcgcgcagc acctgctcga
     2221 ccgcgagggg caggtcgacg cggtagccgg cttcggaggc gcggatat
//
LOCUS       DASPXW010000316         4848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1478996, whole genome shotgun sequence.
ACCESSION   DASPXW010000316 DASPXW010000000
VERSION     DASPXW010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4848
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478996"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VEL04_07185"
     CDS             complement(<1..485)
                     /locus_tag="VEL04_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HYT48188.1"
                     /translation="MKKVYADAAAALKGLLRDNIIIAAGGFGLCGIPENFIRALVDSG
                     VKGLTIVGNNAGVDDFGMGLLLKSRQVKKVIASYVGENKEFERQVLAGELDLQLTPQG
                     TLAEKLRAGGAGIPGFYTRTAAGTKLAEGKETKSFEGKEYVLEEGIRSDLSMVKAWKG
                     D"
     gene            complement(482..895)
                     /locus_tag="VEL04_07190"
     CDS             complement(482..895)
                     /locus_tag="VEL04_07190"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HYT48189.1"
                     /translation="MSERRKLVHTERIAIRWGDMDAMGHVNNTVYFRYMEQARIGWFE
                     ALVPEAQAWQTTGIVIVNASCNFRRPINYPGTLEVKVFVGAPGGSSVPTFYELSIEGE
                     IYADGAATVVFIDMQQQKPVRIPEAIRGLLTEAIQ"
     gene            918..2543
                     /locus_tag="VEL04_07195"
     CDS             918..2543
                     /locus_tag="VEL04_07195"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HYT48190.1"
                     /translation="MPRESMQYDVVVVGAGPAGLAAAIRVKQLAAELSVCVLEKGSEV
                     GAHILSGAVMDPRALAELFADWKAMAAPLNSPVTQDRFLFLTEKSFIETPSFLLPACF
                     KNHGNYVVSLGNVCRWLGKQAEAAGIEIFPGFAAAEVLYEGKTVKGVATGDLGISRKG
                     EKTGAYQPGMELHGKYTFFAEGCRGHLGLQLEEKFALRDGADPQVYGIGLKELWEVQP
                     ERHQPGLVMHTAGWPLDAATYGGSFLYHMENRQVAVGFVVGLGYANPYLSPFEEFQRF
                     KTHPQIRTFFENGKRICYGARAITAGGLQSLPKLVFPGGALIGDNAGFLNASRIKGSH
                     CAIKSGMLAAEAACEAVKAGRSGDELAAYPRAWRSSWLYDELHRARNFKPWMSKGLYT
                     GTLMVGVDQVVFRGKAPWTLHHAHADHETLKRKPDVKPIRYPKPDGVLTFDRLSSVFV
                     SNTNHNEDQPVHLTLKDPSIPTRVNLAEYDGPESRYCPAAVYEFVDLDANPRLQINAQ
                     NCVHCKTCDIKDPTQNIVWVAPEGGGGPNYPNM"
     gene            complement(2580..>4848)
                     /locus_tag="VEL04_07200"
     CDS             complement(2580..>4848)
                     /locus_tag="VEL04_07200"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HYT48191.1"
                     /translation="SQQLRQAFASLGKPTVDVAIVDDDPEMVRLLSRMVGALSGRHRV
                     VTAADGREALARARAGRPDLVISDVLMPLMDGYELARRLKDDPASAATAVLFYTAYFG
                     GEDAKQLAQAHGVVRVLVKPSDNDAILAAIEEVLASHAETTIPPKNLDREHLRIMVDQ
                     LLDKTGALEVQQRRIERLNRTLAMLSAVNALIVRASDRDALLDEACRIAVEKGGFRLA
                     AVGLLERASQTLRQAASAGEGSAERELARLKVGTQALIWNDAQSDSFVALPLIVAGET
                     GGVLLLYSQVRDFFDEEEMRLLHELAGDVSFALHHLGQKARMDYLAYHDSLTDLPNRN
                     LFTDRMGQALSAARRGKRFAAAVFLDIERFRMVNETFGRKSGDELLRQLGARLRRATR
                     DQDTVARVGADRFAIAVAGFDQPGDTTHRVIERVNEAVAQPFAVDGAELGVTLKAGIA
                     VFPTDGSTTEALCANAETALARARATGQRFLFYAPEMNARVAESLAMENRLRRALDEG
                     RLALHYQPKIDVGSGAIAGLEALIRWTDPELGVVPPAKFVSLLEETGMILAAGRWALR
                     RAVEDMRRWRAAGLEVPRVSVNVSAIQLRQKDFVDSVLQALGPTGRERPLIDLEITES
                     VLVEDMEESTRKLQTLRRAGVEVSVDDFGTGYCSLSYLARLPVDTLKIDRSFVVRMRD
                     AGYPRNIVAMIVSLAHTLGLKVIAEGVEDDEQLRLLRELGCDQIQGYLISRPLPPDKI
                     EPLLRAGVEHHMICA"
BASE COUNT          819 a         1647 c         1561 g          821 t
ORIGIN      
        1 ttgtcgccct tccaggcctt gaccatcgac aggtcgctgc ggatgccttc ctccagcacg
       61 tattccttgc cctcgaagct cttggtctcc ttcccctcgg ccagcttggt gcccgccgcg
      121 gtccgggtat agaagccggg aataccggca ccgccggcgc gcagcttctc ggccagcgtg
      181 ccctgcggcg tcagctgcag gtcgagctcg cccgccagca cctggcgctc gaactccttg
      241 ttctcgccca cataggaagc gatgaccttc ttcacctggc gcgacttgag cagcagcccc
      301 atgccgaagt cgtcgacccc ggcgttattg ccgacgatgg tgaggccctt cacgccgctg
      361 tccaccagcg cgcggatgaa attctccggg atgccgcaca ggccgaagcc gccggcggcg
      421 atgatgatgt tgtcgcgcag cagccccttc agggcggcgg cggcgtctgc atagaccttt
      481 ttcattggat ggcctctgta agcaaacctc gaatcgcttc cggaatgcgc accggcttct
      541 gctgctgcat gtcgatgaac accaccgtcg ccgccccgtc agcataaatc tcgccttcga
      601 tgctcaactc ataaaaggtc ggcacgctcg agccgcccgg agcgccgacg aataccttca
      661 cttccagcgt gcccggatag ttgatcggcc gcctgaagtt gcacgaggca ttcacgatca
      721 cgatgccggt ggtctgccac gcctgcgcct cgggaaccag cgcttcgaac cagccgatgc
      781 gcgcctgctc catgtagcgg aaatacaccg tgttgttgac gtgacccatc gcatccatgt
      841 cgccccaacg gatcgcgatg cgctcggtgt gcaccagctt gcggcgttca ctcatgagcg
      901 gcgattataa ttcgcgtatg ccgcgcgaat cgatgcagta cgacgtcgtc gtcgtcggcg
      961 ccggtcctgc gggactcgcc gcggccatcc gcgtgaagca gctcgccgcc gagctctcgg
     1021 tgtgcgtgct cgaaaagggc tccgaggtcg gtgcgcacat cctctcgggc gcggtgatgg
     1081 atccacgcgc gctcgccgag ctgtttgccg actggaaggc catggcagcg ccgcttaatt
     1141 cgccggtgac gcaggaccgc ttcctcttcc tcaccgagaa atcgttcatc gagactccat
     1201 cgttcctgct gccggcgtgc ttcaagaatc acggcaatta tgtcgtcagc ctcggcaacg
     1261 tctgccgctg gctcggcaag caggccgaag ccgccggcat cgagattttc cccggatttg
     1321 cagcagcaga ggttttatat gaagggaaaa ccgtaaaagg cgtcgccacc ggcgatctcg
     1381 gcatcagtcg aaagggcgag aagaccggtg cctaccagcc cggcatggag ctccacggca
     1441 aatacacctt cttcgccgaa ggctgccgcg gccacctcgg cctacagttg gaagagaaat
     1501 tcgcgctgcg cgacggcgcc gatccgcagg tctacggcat cggcctgaag gagctgtggg
     1561 aagtgcagcc ggagcgccac cagccggggc tggtcatgca caccgcgggc tggccgctcg
     1621 atgcggctac gtatggcggc tcgttcctct accacatgga gaatcgccag gtcgcggtgg
     1681 gcttcgtcgt cgggctgggc tacgccaacc cctacctctc gccgtttgag gagttccagc
     1741 gcttcaagac ccatccccag ataagaacgt tctttgaaaa cggcaaaaga atctgctacg
     1801 gcgcgcgcgc catcacggcc ggggggctgc agtcgctgcc caagctggtt tttcccggcg
     1861 gcgcgctgat cggcgacaac gccggctttc tcaatgcttc gcgcatcaag ggcagtcatt
     1921 gcgccatcaa atccggcatg ctcgcggccg aagcggcgtg cgaggcggtc aaggccggcc
     1981 gctcgggcga cgagctcgcc gcctacccgc gggcctggcg ctcgagctgg ctctatgacg
     2041 agctgcaccg cgcccgcaac ttcaagccct ggatgtcgaa aggcctgtac accggtacgc
     2101 tcatggtcgg cgtcgaccag gtcgtgtttc gcggcaaggc gccgtggacc ttgcatcacg
     2161 cccacgcgga tcacgagaca ttgaaacgca agccggatgt aaaaccgatt cgctatccca
     2221 aacccgacgg cgtgctgacc ttcgaccggc tgtcctcggt gttcgtctcg aacaccaacc
     2281 acaacgaaga ccagccggtt cacctgacgc tgaaagaccc ctccattccg accagggtca
     2341 atctggccga atacgacggg ccggagagcc ggtattgccc tgctgcggtc tatgaatttg
     2401 ttgatcttga tgcaaaccca aggctgcaga tcaatgcgca gaactgcgtg cactgcaaga
     2461 cctgcgacat caaggacccg acgcagaaca tcgtctgggt cgcccccgaa ggcggcggcg
     2521 gcccgaacta tcctaatatg tgaccaaaat cagggacaga ccacgttttc tgaccaaagt
     2581 caggcacaga tcatgtgatg ctcgacgccg gcgcgcagca gcggctcgat cttgtcgggc
     2641 ggcagcggcc ggctgatgag ataaccctgg atctggtcgc agccgagctc gcgcagcagg
     2701 cgcagctgct cgtcgtcttc gacgccttcg gcgatgacct tcaggccgag ggtgtgcgcg
     2761 agcgacacga tcatggcgac gatgttgcgg ggataaccgg cgtcgcgcat gcgcaccacg
     2821 aagctgcggt cgatcttgag cgtatcgacc ggcagccgcg ccagatagct gagcgagcag
     2881 tagccggtgc cgaaatcgtc caccgagacc tcgacgccgg cgcgccgcag cgtctgcagc
     2941 ttgcgcgtgc tttcctccat gtcctcaacc agcacgctct cggtgatttc gagatcgatc
     3001 agcggccgct cgcgcccggt ggggccgagg gcctgcagca ccgagtcgac gaagtccttc
     3061 tgcctgagct ggatcgccga gacgttcacc gagacgcgcg gcacctcgag ccccgccgcg
     3121 cgccagcgcc gcatgtcctc gaccgccctg cgcagcgccc agcggccggc ggcgaggatc
     3181 attccggtct cctcgagcag cgagacgaac ttggccggcg gcaccacgcc gagctccggg
     3241 tcggtccagc ggatcagggc ttccaggccg gcgatggcgc cgctgccgac atcgatcttc
     3301 ggctggtagt gcagcgcgag gcgcccttcg tccagggcgc ggcgcaggcg attctccatc
     3361 gccagcgact cggcgacgcg cgcgttcatt tcgggcgcgt agaaaaggaa gcgctggccg
     3421 gtcgcccgcg cccgtgcgag cgcggtctcg gcgttcgcgc acagcgcttc ggtggtcgag
     3481 ccatcggtcg gaaacaccgc gatgcccgcc ttgagcgtga cgccaagctc ggcgccgtcg
     3541 accgcgaacg gctgcgcgac cgcctcgttc acgcgctcga tcacccggtg cgtggtgtcg
     3601 cccggctggt cgaagcccgc caccgcgatg gcgaagcgat cggcgccgac gcgcgccacc
     3661 gtgtcctggt cgcgcgtcgc gcgccgcagg cgggcgccaa gctggcgcag cagctcgtcg
     3721 cccgacttgc gcccgaaagt ctcgttcacc atgcgaaagc gctcgatgtc gaggaacacg
     3781 gcggcggcaa agcgcttccc tcggcgcgcc gcgctgaggg cctgccccat gcggtcggtg
     3841 aacaggttgc ggttcggcag gtcggtgagc gaatcgtggt acgcgaggta atccatccgc
     3901 gccttctgcc cgaggtgatg cagggcgaac gagacgtcgc ccgcgagctc gtgcaagagg
     3961 cgcatctcct cctcgtcgaa gaaatcgcgc acctgcgagt agagcagcag cacacccccg
     4021 gtctcgccgg cgacgatcag cggcagcgcg acgaacgagt cgctctgggc gtcgttccag
     4081 atcagcgcct gcgtgccgac cttcaggcgc gccagctcgc gctcggcgct gccctcgccg
     4141 gcggaggcgg cctgcctgag cgtctggctc gcgcgctcca gcagcccgac cgccgcgagc
     4201 cggaagccgc ccttttctac cgcgatgcgg caggcctcgt cgagcagcgc atcgcggtcg
     4261 gaggcgcgca cgatgagcgc gttcactgcg ctaagcatcg ccaaggtacg gttgaggcgc
     4321 tcgatgcggc gctgctgcac ctcgagcgcg ccggtcttgt cgagcagctg gtcgaccatg
     4381 atgcgcagat gctcgcggtc gaggttcttg gggggaatgg tggtttcggc gtgcgaggcg
     4441 aggacttctt cgatcgcggc caggatggcg tcgttgtctg agggcttgac cagcacccgt
     4501 acgacgccgt gcgcctgcgc cagctgcttc gcatcctcgc cgccgaagta ggcggtatag
     4561 aaaagcaccg cggtcgcggc gctcgcgggg tcgtccttca ggcgccgcgc cagctcgtag
     4621 ccgtccatca aaggcatcag cacgtccgag atcaccaggt cggggcgccc cgcgcgtgcg
     4681 cgggcgagcg cttcgcggcc gtcggcggcg gtcacgaccc ggtggcggcc agagagcgca
     4741 ccgaccatcc gggagagcag ccgaaccatc tccgggtcgt cgtcgacgat cgcgacgtcg
     4801 acggtcggct tgccgagcga ggcgaaggcc tggcgcagct gctggctg
//
LOCUS       DASPXW010000317         2174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1356971, whole genome shotgun sequence.
ACCESSION   DASPXW010000317 DASPXW010000000
VERSION     DASPXW010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1356971"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VEL04_07205"
     CDS             <1..339
                     /locus_tag="VEL04_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003261859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HYT48192.1"
                     /translation="AYPHHILNIHPSLLPAFPGLDTHARALAAGVKLHGCTVHFVTAE
                     VDHGPIVIQAAVSVRPGDSAAALAARVLEQEHVVYPRAARWFVEDRLVLQDGIVTVKG
                     NDAQLVVSAD"
     gene            323..991
                     /locus_tag="VEL04_07210"
     CDS             323..991
                     /locus_tag="VEL04_07210"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HYT48193.1"
                     /translation="MYLLISAAGLAQAALPAQVEIEYELSCDGRGLAEVVERLEQGGG
                     SYQLTETWKGKGLYALLGTAKRTSRGSISEGVLRPLEFADERSGRKTARAWFDWQAKT
                     VTMQYKGNRGTEPMPPNTQDRLSFLLALALMPGKSQSVKFSIFDGRGQSRHEYEVGSR
                     ELLKTPAGEFDTVKITRRYEPGAKDRAELWLAREQGHLPVRLLAVEKDGTRCEHLARR
                     ISRP"
     gene            988..1128
                     /locus_tag="VEL04_07215"
     CDS             988..1128
                     /locus_tag="VEL04_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48194.1"
                     /translation="MTAAARLTPRLVAHAVAAASKILQFAGPADDVLSRYFRSQRELG
                     QH"
     gene            1150..>2174
                     /locus_tag="VEL04_07220"
     CDS             1150..>2174
                     /locus_tag="VEL04_07220"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HYT48195.1"
                     /translation="MFAVLRRKRSLEAAAGSAEPQALVAAALLRVRGLSGRALAGLVD
                     EALIARVRAARSEALADAVRADLPDWLWQRLAAAHGHDEAMRIAQGLANAAPLDLRVN
                     LARLGRDAALARLGADGIEGSPTAYSPAGIRLAGKPAINRHPLFRDGLIEVQDEGSQL
                     LAWLLAPRRGEMIADYCAGAGGKTLAAAMLMRGSGRIYALDTSVKRLAALAPRAARAG
                     ISNVHSIALDGDNDVRAKRLAGKLDRVLVDAPCSGFGTLRRNPDLKWRHGPDAIGELA
                     QKQLRILESASRLVKPSGRLVYATCSILAEENEAVTVAFQATHRDFEPLACGELLAAQ
                     RIPLDTG"
BASE COUNT          338 a          748 c          759 g          329 t
ORIGIN      
        1 gcgtatcccc accacatctt gaacattcat ccctcgctgc tgccggcgtt ccccggcctc
       61 gatacgcacg cgcgggcgct cgccgcgggc gtgaaactgc atggctgcac cgtgcatttc
      121 gtcaccgcag aggtcgacca cggcccgatc gtgatccagg ccgcggtgag cgtgcggccg
      181 ggcgacagcg ccgcagcgct ggccgcgcgc gtgctggagc aggagcacgt cgtctacccg
      241 cgcgccgcgc gctggttcgt cgaggaccgt ctggtgctgc aggatggtat cgtcacagtg
      301 aaaggcaatg atgcgcaact ggttgtatct gctgattagc gccgcgggcc tggcgcaggc
      361 ggcactgccc gcccaggtcg agatcgagta cgagttgtcc tgcgacggcc gcggtctcgc
      421 ggaagtcgtc gagcggctcg agcaaggcgg cggcagttac cagctcaccg agacctggaa
      481 gggcaaggga ctttacgcgc tcctcggaac ggcgaagcgc acgagccgcg gcagcatcag
      541 cgagggtgtc ctgcgcccgc tggagtttgc cgacgagcgc tccggccgca agactgcacg
      601 cgcctggttc gactggcagg cgaagacggt gaccatgcag tacaagggca accggggcac
      661 tgagccgatg ccgcccaata cacaggatcg cctgtccttt ctccttgccc tcgcgctgat
      721 gcccggcaaa tcgcagtcgg tgaagttcag cattttcgac ggccgcgggc agtccagaca
      781 cgaatacgag gtcggcagtc gcgagctgct gaagactccc gccggagaat tcgataccgt
      841 gaaaatcacc cggcgctacg agcccggggc caaggaccgg gccgagctct ggctagcgcg
      901 cgagcaaggg catcttccgg tgcggctgct cgcggtcgaa aaggacggca cgcgctgtga
      961 gcacctagcc aggcgcatct cccgaccgtg actgcggccg cgaggctgac gccgcggctc
     1021 gtggcgcacg cggtcgccgc ggcttcgaaa atcctgcagt ttgccgggcc cgccgacgat
     1081 gtcctgtcgc gctatttccg cagccagcgc gagcttgggc agcactagcg ggcattcatc
     1141 gccgaggcgg tgttcgcggt tctgcggcgc aagcgctcgc tcgaggcggc agccggctcc
     1201 gccgaaccgc aagcgcttgt cgccgccgcg ctgttgcgcg tgcgcggcct ttccgggcgc
     1261 gctctagcgg gcttggtcga cgaggcgctg attgcccgcg tccgcgcggc gcgcagcgaa
     1321 gcacttgccg atgcggtgcg tgcggacttg cccgattggc tgtggcagcg cctcgctgct
     1381 gcgcacgggc acgacgaggc aatgcgcatc gcgcaggggc tggcgaacgc agcgccgctc
     1441 gacctgcgcg tcaatctggc gcgcctcggg cgcgacgcag cgctcgcgcg cctcggcgcc
     1501 gacggcatcg aaggctcgcc gacagcttat tcgccggcgg gtatcaggct cgccggcaag
     1561 cctgcgatca accgtcatcc gctcttccgc gacgggctga tcgaggtgca ggacgagggc
     1621 agccagcttc tcgcctggct gctcgcgccg cggcgcggcg agatgattgc cgactactgc
     1681 gccggcgccg gcggcaagac gctcgccgcg gcgatgctga tgcgcggcag cgggcgcatc
     1741 tacgcgctcg acacgtcggt gaagcggctc gccgcgctcg ccccgcgggc ggcgcgcgcg
     1801 ggtatcagca acgtgcattc gatcgcgctc gacggtgaca acgacgtgcg cgccaagcgg
     1861 ctagccggca agctcgaccg tgttctggtt gacgcgccgt gcagcggctt cggcacgctg
     1921 cggcgcaatc cagacctgaa atggcgccac ggccccgacg cgatcggcga gctggcgcaa
     1981 aagcagctgc gcatactcga atccgcctcg cgcctggtaa aaccttcggg ccggctggtc
     2041 tacgcgacct gcagcatcct cgcggaggag aacgaggcgg tcacggtcgc cttccaggcc
     2101 acgcatcgcg atttcgagcc gctcgcgtgc ggcgagctgc tcgcggcaca acgcatcccg
     2161 ctggacaccg gcga
//
LOCUS       DASPXW010000318         2465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_218228, whole genome shotgun sequence.
ACCESSION   DASPXW010000318 DASPXW010000000
VERSION     DASPXW010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218228"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..862)
                     /locus_tag="VEL04_07225"
     CDS             complement(29..862)
                     /locus_tag="VEL04_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HYT48196.1"
                     /translation="MIIEPLAGALGAEIRGVSLTRADDGAWQEIQRAFLEYAVLVFRD
                     QALEPADLMRVGAHFGEPCFYPFVTGLDGYPYLFEVVKEEDERTNFGGNWHSDTTYLR
                     EPPLATLLHALETPAHGGDTLFASTRAAYDALSAGMRGLVDRLIGVNSAALKHGGGRS
                     AMHKKIAGMKVHDTETAEHYEAEHPVARTHPETARKALYVSRSHTIHFKDMSEEESRP
                     LIDWLQAHQTRPEFTCRVRWAPGTLTVWDNRCTQHNAINDYHGQRRRMRRLTVGAQTP
                     R"
     gene            874..1929
                     /locus_tag="VEL04_07230"
     CDS             874..1929
                     /locus_tag="VEL04_07230"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HYT48197.1"
                     /translation="MTKRLAIVGAGALGGYVGGSLAHLGHDVTLIDPWPEHVEKIRAR
                     GLELDGMTPEERFVVKSVKTLHLTEVQSLARQPVDVAMVAVKSYDTAWATALIAPYLS
                     PQGFVVSLQNCLNEETIAGVVGWGRTVGVIASLISVDLYEAGRIRRTVAKGGDKHTVF
                     RAGEPHGRVTARVEELVEWFRGIDSARATTNLWGERWSKLVQNGMGNGVTAATGLTSG
                     DCLRNTAIRRFQIALAGEGVRVGQALGYQLEKIRGIEPERLARAAEGDAQALSEVEAA
                     LIPKTGANPRGDIQRPSMAQDILKGRRTEIDAMNGFIARKGAELGVPAPSHAKLTELV
                     TRIERGELKPSPSHLES"
     gene            2013..>2465
                     /locus_tag="VEL04_07235"
     CDS             2013..>2465
                     /locus_tag="VEL04_07235"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HYT48198.1"
                     /translation="MNRRDFISSMAGVAAAWPCLTIAQTSSRPTIGYLDATSPAAAAR
                     FVAAFRDGLKASGYIEGQNLSIQYRWAEGEFSRLPAMAADLVKQNVTVIVSATLPATL
                     AAKAAAKGGIPIVFALSADPVEFGVVSRLNNPDGNITGVTQVLGALGAK"
BASE COUNT          420 a          784 c          840 g          421 t
ORIGIN      
        1 ctccgcttca tccacctgcg gaaataagct agcgcggtgt ttgcgcaccc acggttaagc
       61 gccgcatgcg tcggcgctgg ccgtgatagt cgttgatcgc attgtgctgg gtgcagcggt
      121 tgtcccacac ggtgagcgtc cccggcgccc agcgcacgcg gcaggtgaat tccggccgcg
      181 tctggtgcgc ctgcagccag tcgatgagcg ggcggctctc ctcctcgctc atgtccttga
      241 agtggatggt gtggctgcgg ctgacataca aggccttgcg ggcggtctcg ggatgcgtgc
      301 gcgccaccgg atgctcggct tcgtagtgct cggcggtctc ggtatcgtgc accttcatgc
      361 cggcgatctt cttgtgcatc gcgctccggc cgccgccatg cttgagtgcc gcgctgttga
      421 cgccaatgag ccggtcgacc agcccgcgca tgccggcgga aagtgcatcg taggcggcgc
      481 gcgtgctggc aaacagcgtg tcgccgccgt gcgccggcgt ctcgagcgca tgcagcaggg
      541 tggcgagcgg cggctcgcgc agataggtgg tgtcgctatg ccagttgccg ccgaaattgg
      601 tcctttcgtc ctcttccttc accacctcga agaggtacgg gtagccgtcg aggccggtga
      661 cgaaaggata gaagcacggc tcgccgaagt gcgcgccgac gcgcatcagg tcggcaggct
      721 cgagcgcctg gtcgcggaac acgagcaccg cgtactcgag aaacgcccgc tggatctcct
      781 gccaggcgcc gtcgtctgcc ctggtgagcg agacgccgcg gatttcggct cccagcgcgc
      841 cggccagcgg ctcgattatc atcgggcaag tttatgacaa aacggcttgc catcgtcggg
      901 gcgggagcgc tgggaggcta tgtcggcggc agccttgcgc atctcgggca cgacgtgacg
      961 ctgatcgacc cgtggccgga gcacgtcgag aagatccgcg cgcgcggcct cgagctggac
     1021 ggcatgacgc ccgaagagcg attcgtggtc aaaagcgtga aaacgctgca tctcaccgag
     1081 gtgcaatcgc tcgccaggca accggtcgac gtcgcgatgg tcgccgtcaa gtcctacgac
     1141 accgcctggg cgacggcgct gatcgcgccc tacctctcgc cgcagggctt cgtggtgtcg
     1201 ctgcagaact gcctgaacga ggaaaccatt gccggcgtgg tcggctgggg acgcaccgtg
     1261 ggagtgattg cctcgctcat ttcggtggac ctgtacgagg ccgggcgcat ccgaaggacg
     1321 gtggccaagg gcggcgacaa gcacaccgtg ttccgcgccg gcgagccgca cgggcgcgtc
     1381 accgcccgcg tcgaagagct ggtcgaatgg ttccgcggca tcgacagcgc aagagcgacc
     1441 accaatctct ggggcgagcg ctggtcgaag ctggtgcaga acggcatggg caacggcgtc
     1501 accgcggcga ctgggctcac ctcgggcgac tgcctgcgca acaccgccat ccgccgcttc
     1561 cagatcgcgc tcgcgggcga aggcgtgcgc gtcggccagg cgctcggcta ccagctcgag
     1621 aagatccgcg gcatcgagcc cgagcgcctc gcgcgcgcgg ccgagggcga cgcgcaagcg
     1681 ctgtcggaag tcgaggcggc gctgattccg aaaacgggcg ccaacccgcg cggcgacatc
     1741 cagcgtccgt cgatggcgca ggacatcctc aagggcaggc gcaccgagat cgacgccatg
     1801 aacggcttca tcgcacgcaa aggcgccgag ctcggcgtgc cggcaccgag ccacgcgaag
     1861 ctcaccgagc tcgtgacgcg catcgagcgc ggcgaattga agccctcgcc ctcgcatttg
     1921 gaaagttagg gaagaccgga ggtgcttcgg tttgaatgtc tgttttctaa agcggacgtt
     1981 cacggtgtag gctagcgtga aggagcccag ctatgaatcg gcgtgatttc atatcttcga
     2041 tggcgggcgt agcggctgcg tggccctgtc tcaccattgc acaaacgagc agtcgaccga
     2101 ccattggata tctcgacgcc acttctcccg ctgctgccgc acgctttgtt gcggcattcc
     2161 gtgatggact gaaagcgagc gggtatatcg agggtcaaaa cctgtcgatc caatatcggt
     2221 gggccgaggg agagttttcg cgcctgccgg cgatggctgc ggacctcgtc aagcagaacg
     2281 tgacggtaat cgtgtcggcg actcttccgg cgaccttggc agcgaaggcc gccgcaaagg
     2341 gcggcatacc gatcgtattc gctctcagcg ccgatcccgt cgagttcggc gtcgtctcga
     2401 ggctcaacaa tcccgacggc aacatcaccg gggtcactca ggtgctgggc gctctcggag
     2461 cgaag
//
LOCUS       DASPXW010000319         6273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_846926, whole genome shotgun sequence.
ACCESSION   DASPXW010000319 DASPXW010000000
VERSION     DASPXW010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6273
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846926"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1567
                     /locus_tag="VEL04_07240"
     CDS             <1..1567
                     /locus_tag="VEL04_07240"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HYT48199.1"
                     /translation="LKKVTVADVQRVAEQYFKPANRVLGLFVPTERADRAEIPPTPAL
                     EIALADYRGGESVQQGEAFDASPKNIESRVIRRQLSNGIRVALLAKRTRGGRVVAGLT
                     LHWGDEKRLMNREAACDFAGAMLTRGTQNKTRAELKDAFERLNAAVSIGGEGASIEVR
                     RENLVPALRLAAEALREPSFPAAEFEEMKRAALAGAEEQKSDPAALAGVRLARHLQDY
                     PRGHPNYTPTIDERIEWLRRTTLEGAVACYRELYGATGADFVAVGDFDPEEVARAVDE
                     LFGAWRAPTPFERVPLRYFDRPALHNELPAPDKANAVLRAGVNVRMREDDADFPAMLL
                     ANYLLGGSPTARLPGRVREKEGLSYSTYTAFTSNAFDEAAVFRVASSFAPQNRARVEQ
                     AIREEIERAVREGFSAAEVEAGRQAVLEARRLARTQDRSLASRLGAYLFAKRTFAWDI
                     ELERNLASLSAAEVSAALKKHIDPARLSVIMAGDFKKPSAASSAGRESPPAAAPGAAA
                     TPALSPRLSAGSD"
     gene            complement(1464..1841)
                     /gene="mscL"
                     /locus_tag="VEL04_07245"
     CDS             complement(1464..1841)
                     /gene="mscL"
                     /locus_tag="VEL04_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HYT48200.1"
                     /translation="MSWLREFREFAVKGNVLDLAVAVIIGGAFGKIVSSLVEDMVMPA
                     VGVLIGGFDFKNLFLQVGAAKIGYGKFIQSGVDFLIIAAAIFLAVKLINRFRQKAEEK
                     APASPPRQEQLLEEIRDLLKTRH"
     gene            complement(1838..2338)
                     /gene="greB"
                     /locus_tag="VEL04_07250"
     CDS             complement(1838..2338)
                     /gene="greB"
                     /locus_tag="VEL04_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HYT48201.1"
                     /translation="MSKAFIKETGHGKNYITPSGYARLEAELRQLVEAERPAVVKTVA
                     WAASNGDRSENADYIYGKRRLREIDRRVRFLIKRLEAAQIVDASGRDTDQVFFGATVK
                     VKSKNEVKEITIVGVDEVDLARGRVSWISPIAKALIKAREGDRVALQTPAGKETLEIM
                     EVRYPL"
     gene            2416..3129
                     /locus_tag="VEL04_07255"
     CDS             2416..3129
                     /locus_tag="VEL04_07255"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HYT48202.1"
                     /translation="MAEQLDFLTKLRIRELILTGLITLTAVLANLPHDYVQETLGVSH
                     NLLLAILAIAIILGLFLYLRFGLFVVVLLLIAGANMPEQIAEGLNISKVPIVLALVAL
                     VGVGLINYVVKLMPTGLEPKPREKSAEGVKALFYGIEKNNLVYAQKVLSMNFDPNLLH
                     DNGYTPLAYAAMKGSLPMIELLLRNGADPLQLTKEGDTPVELALRMGHAEVADALKRA
                     RQTGTEPPAARQRTAQTAS"
     gene            3189..6023
                     /locus_tag="VEL04_07260"
     CDS             3189..6023
                     /locus_tag="VEL04_07260"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HYT48203.1"
                     /translation="MEIQLSAQASAKEQEICRDTLLEKYAKGNEASVDEVRRRVARAL
                     AQAEPEEQRAHWERRFLQAQEDGFIPAGRINSAAGVQLQATLINCFVQPVGDSISEVV
                     DGKPGIYTALLEAAETMRRGGGVGYDFSAIRPKGAEVKGTHSRASGPVSYMRVFDRSC
                     ETVESAGSRRGAQMGVLRCDHPDIEDFIQAKDAGDLANFNISVGVTDDFMVAVEKDAS
                     IELVHKAKPWPGNPEAYQRGDGAWVYRKLRARELWDQIMRSTYDHAEPGILFLERINR
                     DNNLNYCETIEATNPCAEQPLPPYGCCCLGSIDLTRFIKQPFTERSAFDFEHFGRTIE
                     TSVRMLDNVLEVTAWPLEQQRKEAMAKRRVGLGFTGLGDALILLRLRYDTEEARAMAA
                     RISESMRDRAYRASVELAKERGAFPLFNADLYLSGTSFASRLPLPLKHQIQKHGIRNS
                     HLLSIAPTGTVSLAFADNASNGIEPPFSWTYTRRKRVQDGMQEYPVEDHAWRRYKTLG
                     GNMDELPPYFVTAHDISARAHEQMVAAVAPYIDTSISKTVNVPEDYPYAEFQDLYLHA
                     WKSGLKGLATYRPNAVLGAVLQINKPQDFVHDDVNRRIAVKSVPAPVLASLRWPGRPE
                     LPAGNPSWTFMLEHPHSRFAVFVSHLENGAPHPFEVWINGSEQPRGLGALAKTLSMDM
                     RANDRAWLRLKLDTLAKAGGGDAFDMPFPPRGEKQHMPSLVSAFAQIVRWRVEQLHAG
                     LDSDFSLSGGEGPTPVMDALFALKEPKTGPDGTISWTVDVLNPRTGDDFVLGLKEISL
                     PDGNTRPYSVWLSGEYPRALDGLCKILSLDMRVLDPAWIGMKLKKLLEFPEPLGDFMA
                     FVPGSRRQQTYPSTVSYLARLILHRYQMLGLLGEDGAPKQQMGILESPSEKRPLILAG
                     AKCPECGNKTLIRRDGCDYCTACGFVGICG"
     gene            complement(6020..>6273)
                     /locus_tag="VEL04_07265"
     CDS             complement(6020..>6273)
                     /locus_tag="VEL04_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HYT48204.1"
                     /translation="RVFKNRPVGLIGASPGAYGTIQAQGAWLPVFRTLGMRPWFGQLM
                     YVGNAAKVFDASGKLVDEAIRTRLRNYVRGFTEFVASGS"
BASE COUNT         1108 a         2147 c         2014 g         1004 t
ORIGIN      
        1 gttgaaaaaa gtcaccgttg ccgatgtgca gcgcgtggcg gagcaatact tcaagcctgc
       61 caatcgggtg ctgggcctgt tcgtccccac cgagcgtgcg gatcgggccg agatcccgcc
      121 gacccccgcc ctcgagattg cgctcgccga ctaccggggc ggcgagagcg tgcagcaggg
      181 cgaggccttc gatgcgtcgc cgaagaacat cgagtcgcgc gtcatccgcc gccagctctc
      241 caacggcatc cgcgtcgcgc tgcttgccaa gcgcacgcgc ggcggccgcg tggtggcggg
      301 cctgacgctg cactggggcg acgagaaacg cctgatgaac cgcgaggcag cctgcgactt
      361 cgccggcgcc atgctcacgc gcggcacgca gaacaagacc cgcgccgagc tgaaggacgc
      421 tttcgagcgc ctcaacgccg cggtgtcgat cggcggcgag ggcgcgagca tcgaagtgag
      481 gcgcgagaac ctggtgccgg cgctgcgcct cgccgcggag gcgctgcgcg agccgtcgtt
      541 cccggccgcg gagttcgagg aaatgaagcg cgccgcgctc gccggcgccg aggagcagaa
      601 gagcgacccg gcggcgctcg ccggcgtgcg cctcgcgcgc cacctgcagg actatccgcg
      661 cggccacccc aactacaccc cgaccatcga cgagcgcatc gagtggctgc gccgcactac
      721 cctcgagggc gcggtcgcct gctaccgcga gctctatggc gcgaccggcg ccgacttcgt
      781 cgcggtcggc gatttcgacc ccgaggaggt cgcgcgcgcg gtcgacgagc tgttcggcgc
      841 ctggcgcgcg cccacgccct tcgagcgcgt gccgctgcgc tacttcgacc gcccggcgct
      901 gcacaacgag ctgcccgctc cggacaaggc gaacgcggtg ctgcgcgccg gcgtcaacgt
      961 gcgcatgcgc gaggatgacg ccgactttcc ggcgatgctg ctcgccaact acctgctcgg
     1021 cggctcgccc accgcgcgcc tgcccgggcg cgtgcgcgag aaggaaggcc tctcgtacag
     1081 cacctacacc gcgttcacct cgaatgcgtt cgacgaggcg gccgtgttcc gcgtggcgtc
     1141 gagcttcgcg ccgcagaacc gcgcgcgcgt cgagcaggcg atccgcgagg agatcgagcg
     1201 cgcggtgcgc gaaggcttca gcgcggcgga ggtcgaggcc ggcaggcagg cggtgctcga
     1261 ggcgcggcgc ctcgcgcgca cccaggaccg ctcgctcgcg agccggctcg gcgcctatct
     1321 cttcgccaag cgcactttcg cctgggacat cgagctcgag cgcaacctcg cctcgctgag
     1381 cgccgccgag gtcagcgccg ccctgaagaa gcacatcgat ccggcgcggc tgtcggtcat
     1441 catggccggg gacttcaaga aacctagtgc cgcgtcttca gcaggtcgcg aatctcctcc
     1501 agcagctgct cctggcgcgg cggcgacgcc ggcgctttct cctcggcttt ctgccggaag
     1561 cgattgatca gcttcacggc gaggaagatc gcggccgcga tgatgaggaa gtcgacgccg
     1621 ctctggatga acttgccgta gccgatcttc gccgcgccaa cctgcaggaa cagattcttg
     1681 aaatcgaagc cgccaatcag cacgccgacc gcgggcatca ccatgtcctc gaccagcgac
     1741 gacacgatct tgccgaaggc gccgccgatg atcacggcca cggccaggtc gagcacattg
     1801 cccttgaccg cgaactcgcg gaactcgcgc agccagctca tagcgggtat ctgacctcca
     1861 tgatttcaag agtttccttg cctgccgggg tctgcagcgc gacgcgatcg ccctcgcgcg
     1921 ccttgatgag cgctttcgcg atcggcgaga tccacgacac gcgaccgcgg gcgaggtcta
     1981 cctcgtcgac gccgacgatg gtgatttctt tcacttcgtt tttcgacttt actttcacgg
     2041 tggcgccaaa gaaaacctgg tcagtgtccc ggccgctggc gtcgacgatc tgcgccgcct
     2101 cgagccgctt gatgaggaag cgcacgcgcc ggtcgatctc gcgcagccgg cgcttgccgt
     2161 agatgtaatc ggcgttctcc gaacggtcgc cgttcgaggc ggcccaggcg acggtcttca
     2221 ccaccgcggg gcgctccgcc tcgacgagct gcctcagctc ggcttcgagg cgcgcgtagc
     2281 ccgagggggt gatgtaattt tttccgtgcc cggtttcctt gataaaagct ttggacatgg
     2341 cgctcgggcc aaattctatc tccgcttgca agcggggcgc caagctctca aaattgcggg
     2401 ttcagggggg aagaaatggc tgaacagctc gatttcctga cgaagctccg catccgcgag
     2461 ctgatcctga cgggcctcat cacgctcacc gcggtgctgg caaacctgcc gcacgactac
     2521 gtccaggaga cgctcggcgt cagccacaac ttgctgctcg cgatcctcgc catcgcgatc
     2581 atcctgggac tgttcctgta cctcaggttc ggcctgttcg tggtggtgct gctgctgatc
     2641 gccggcgcca acatgcccga gcaaatcgcc gaagggctga acatctcgaa agtgcccatc
     2701 gtcctggcgc tggtggcgct ggtgggcgtc ggcctgatca actatgtggt caagctgatg
     2761 ccgaccgggc tcgagcccaa gccgcgcgag aagagcgcag agggcgtcaa ggcgctgttc
     2821 tatggcatcg agaagaacaa cctggtgtac gcgcagaaag ttctgtcgat gaacttcgac
     2881 ccgaacctgc tccacgacaa cggctacacg ccgctcgcct acgcggcgat gaagggcagc
     2941 ctgccgatga tcgagctcct gctgcgcaac ggcgccgatc cgctgcagct caccaaggaa
     3001 ggcgacacgc cggtcgagct cgccctgcgc atgggccacg ccgaggtcgc cgatgcgctc
     3061 aagcgcgccc gccagacagg taccgagccg ccggctgccc gacagcgcac agcacagaca
     3121 gctagctgag acgcaacaat aacttacggc gcttgacgcc agtcaagcct caagttctat
     3181 aatactgtat ggaaatccag ctgtccgccc aggcaagcgc caaagagcag gaaatctgcc
     3241 gcgacacgct gctggagaaa tacgccaagg gaaacgaggc ttcggtcgat gaagtgcggc
     3301 gccgcgtggc gcgcgccctc gcgcaggccg agcccgagga gcagcgcgcc cactgggagc
     3361 gtcgcttcct gcaggctcag gaagacggct tcatcccggc cgggcgcatc aactcggccg
     3421 ccggggtgca gctccaggcc acgctcatca actgcttcgt gcagccggtc ggcgactcga
     3481 tctcggaagt ggtcgacggc aagcccggca tctacaccgc gctgctcgaa gccgccgaga
     3541 ccatgcggcg cggcggcggc gtcggctacg acttctccgc catccggccc aagggcgccg
     3601 aggtcaaggg cacgcattcg cgcgcgagcg gcccggtgtc gtacatgcgc gtcttcgacc
     3661 ggtcctgcga gacggtcgag tcggctggtt cgcgacgcgg tgcgcagatg ggcgtgctgc
     3721 gctgcgacca tcccgacatc gaggacttca tccaggccaa ggacgcgggc gacctcgcca
     3781 acttcaacat ctccgtcggc gtgacggacg atttcatggt tgctgttgaa aaagacgcat
     3841 ccattgaatt ggtgcataaa gcaaaaccct ggcccggcaa ccccgaggcc taccagcgcg
     3901 gcgacggcgc gtgggtctac cgcaagctgc gcgcgcgcga gctgtgggac cagatcatgc
     3961 gctcgaccta cgaccatgcc gagcccggca tcctgttcct cgagcgcatc aaccgcgaca
     4021 acaacctcaa ctattgcgag accatcgagg cgaccaatcc ctgcgccgag cagccgctgc
     4081 cgccttacgg ctgctgctgt ctgggctcga tcgatctgac tcgtttcatc aaacaaccat
     4141 tcaccgaaag aagcgcgttt gatttcgagc atttcggcag gaccatcgag acctcggtgc
     4201 gcatgctcga caacgtgctt gaggtcaccg cctggccgct cgagcagcaa cggaaggaag
     4261 cgatggcgaa gcgccgcgtc gggctcggct tcaccggcct gggcgacgcg ctcatcctgc
     4321 tgagattgag atatgacacc gaggaggcgc gcgccatggc ggcgcgcatc tcggagagca
     4381 tgcgcgatcg cgcctaccgg gcgtcggtcg agctggcgaa ggagcgcggc gcgttcccgc
     4441 tgttcaacgc ggatctgtat ctgtccggca cgagcttcgc ctcgcgcctg cctttgcctt
     4501 taaagcacca gattcagaaa cacggcatcc gaaattcgca tctgctgtcg atcgcgccaa
     4561 ccggcaccgt cagcctcgcc ttcgccgaca acgcctcgaa cggcatcgag ccgccgttct
     4621 cctggaccta cacgcgcagg aagcgcgtgc aggacggcat gcaggaatac ccggtggagg
     4681 accacgcctg gaggcgctac aagacgctcg gcggcaacat ggatgagctg ccgccctact
     4741 tcgtcaccgc gcacgacatc agcgcgcgcg cgcacgagca gatggtggcg gcggtggcgc
     4801 cgtatatcga caccagcatc tcgaagacgg tgaacgtacc cgaggactat ccgtacgccg
     4861 agttccagga cctctacctg cacgcctgga agtcgggcct gaaagggctc gcgacgtacc
     4921 ggccgaacgc ggtattagga gcagttttac aaatcaacaa gccacaggat ttcgtccacg
     4981 acgacgtcaa ccgccgcatc gcggtgaaat cggtccccgc cccggtgctc gcctcgctgc
     5041 gctggcccgg ccgccccgag ctgccggcgg gcaacccgtc ctggaccttc atgctcgagc
     5101 atccgcactc gcgcttcgcg gtgttcgtga gccacctcga gaacggcgcg ccgcacccct
     5161 tcgaggtctg gatcaacggc agcgagcagc ctcgcgggct gggcgcgctg gccaagaccc
     5221 tctcgatgga catgcgcgcc aacgaccgcg cctggctgcg tctcaagctc gacacgctcg
     5281 ccaaagccgg aggcggcgac gccttcgaca tgccgttccc gccgcgcggc gagaagcagc
     5341 acatgccttc gctggtttcg gccttcgcgc agatcgtgcg ctggcgcgtc gagcagctcc
     5401 acgcggggct ggacagcgac ttttcgttgt cggggggcga aggaccgacc ccggtgatgg
     5461 acgcgctgtt cgcgctgaag gagccgaaga ccggccccga cggcacgatc tcctggacgg
     5521 tcgatgtcct caacccgcgc accggcgacg attttgtcct gggtttaaaa gaaatatctt
     5581 taccggatgg aaataccagg ccgtattcgg tctggctgtc gggtgaatat ccgcgcgcgc
     5641 tcgacgggct gtgcaagatc ctgtcgctcg acatgcgcgt gctcgacccg gcgtggatcg
     5701 ggatgaagct gaagaagctg ctcgagtttc ccgagccgct cggcgacttc atggccttcg
     5761 tgccgggcag ccgcaggcag cagacctatc cctcgaccgt atcctatctc gcgcgcctga
     5821 tcctgcaccg ctaccagatg ctcggcctgc tcggcgagga cggcgcgccg aagcagcaga
     5881 tgggaatcct ggaatcgccc tccgaaaagc gcccgctcat tctcgccggc gcgaaatgcc
     5941 ccgagtgcgg caacaagacg ctgatccgca gggacggctg cgactactgc actgcctgcg
     6001 gcttcgtggg aatctgcggt taggacccgc tggcgacgaa ttcggtgaat ccccgcacgt
     6061 aattgcggag ccgcgtgcgg atcgcctcat cgaccagctt gccggaggcg tcgaacacct
     6121 tggccgcgtt gccgacgtac atcagctggc cgaaccaggg ccgcatgccg agggtgcgga
     6181 aaaccggcag ccacgcgccc tgcgcctgga tcgtgccgta agcgccgggc gaggcgccga
     6241 tcagcccgac cggccggttc ttgaacactc gcc
//
LOCUS       DASPXW010000320         3547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_724328, whole genome shotgun sequence.
ACCESSION   DASPXW010000320 DASPXW010000000
VERSION     DASPXW010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724328"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VEL04_07270"
     CDS             <1..280
                     /locus_tag="VEL04_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HYT48205.1"
                     /translation="VDLNETMVRAIARQAEAERERRAKVIHAEGELQASEKLLAAAEM
                     LSKRAEAMQLRYLQTLTQIAGDKSSTIVFPVPMDILGPLIQAATRPKT"
     gene            323..1255
                     /locus_tag="VEL04_07275"
     CDS             323..1255
                     /locus_tag="VEL04_07275"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HYT48206.1"
                     /translation="MTQERRAASELQAKLRAGEFVVTAEITPPVSTDPAEFLRRALPL
                     KGLATAVNVTDGAGAKVHLSSLAAAHFLVQSGIEPIFQMTCRDRNRLALQGDILGAVS
                     LGIHNILALAGDDPKAGDQPEAKAVYDLDSKGLLAMAHAMRSQNKLPSGTKIDGGLHL
                     VLGAADVPIDPPPGWEPNSLRAKAAAGADFVQTQFCMDMGVVRRYAARLLEAGIKLPI
                     LIGVAPIPSARSARWMREKLFGTLIPDPIIERLEKAREPKSEGRKICVEILRELATIP
                     GVAGAHVMAPMNFAEIPLAIEESGVLRKKRKAAA"
     gene            1252..2013
                     /locus_tag="VEL04_07280"
     CDS             1252..2013
                     /locus_tag="VEL04_07280"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYT48207.1"
                     /translation="MSFTASFVEVDGCRTHLRRGGKGRPLLYLHGASGAPVILPFMEK
                     LAARFDVLVPEHPGYGLSGEPEWLENIHDVAYFYLDFLHQLNLKETTLIGSSMGGWIA
                     MEMAVRDTSRISSLVLVGPAGISAPGVEPADIFLMSPEDVVRNLFFDQKLAEARLAEP
                     MTPEALDLSLKNRHTTARLAWEPRLHDPFLPKWLHRIDVPVKLVWGAEDRILPSAFTK
                     EFSRLMPKAEIHVIPGCGHLPQAEKPDEFCDIVCR"
     gene            2004..3149
                     /locus_tag="VEL04_07285"
     CDS             2004..3149
                     /locus_tag="VEL04_07285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYT48208.1"
                     /translation="MQVVLFHLMPYADLDLEAGRKNGTVWVKLPNKHFDPVKGHKLYN
                     RYLDELEYAEALGFDAVSVNEHHQTAYGLMPSPIVTASALARKTSRVKIAILGSALPL
                     RSHPMMVAEEHAMIDVISGGRLISGFVRGIGAEYHTFGVNPTFSHDRFHEAHDLIIRA
                     WTEPGPFAFHGKHYNLQYVNLWPRPYQKPHPPVWIPSQGSKETIDWASHPERRYTYLQ
                     TFSPVKVVGRYLKQYRDTAKGYGYEAKDNQLGWSLPVYVSSSDQKAKEEAKAHFEAFR
                     NVFVRMPLEMLLPPGYTSRESLKNVMKAKAQMFGDVTLEQALELGLFVCGSPETVRKN
                     LENHWSDMRFGNLLVSCQFGTLPADLTRKNMELFAREVMPQLKVLGK"
     gene            3157..>3547
                     /locus_tag="VEL04_07290"
     CDS             3157..>3547
                     /locus_tag="VEL04_07290"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYT48209.1"
                     /translation="MRGAIFIGVLLAAASFSASPQTYPAKPVRIIVPSAPGGGYDFIG
                     RLAAERFSQEFGQGFVVENRVGAGTLVGTQAAAAAPADGYTLLVGGLANMAFNPGLHK
                     DLRYDPVADFTPIAMVGAFTYALVGRKD"
BASE COUNT          664 a         1166 c         1132 g          585 t
ORIGIN      
        1 cgtcgacctc aacgagacca tggtgcgcgc gatcgcgcgc caggccgagg ccgagcgcga
       61 gcgccgcgcc aaggtgatcc acgccgaggg cgaactgcag gcctccgaga agctgctcgc
      121 cgcggccgag atgctatcca agcgcgccga ggcgatgcag ctgcgctatt tgcagacgct
      181 gacgcagatt gccggcgaca agtcgtcgac gatcgtgttc ccggtgccga tggatatcct
      241 ggggccgctc atacaagccg cgaccaggcc caagacctga cccatacatc cagggaaggt
      301 cagggccgga tatgattttt ccatgactca ggagcggcgc gcggcgagcg aattgcaggc
      361 caagctgcgc gcgggcgagt tcgtcgtcac cgcagagatc accccgcccg tatcgaccga
      421 cccggccgag ttcctgcgcc gcgcgctgcc gctgaaaggc ctcgccaccg cggtcaacgt
      481 caccgacggc gcgggcgcga aggtgcatct gtcgagcctc gcggcggcgc acttcctggt
      541 gcaaagcggc atcgagccga tcttccagat gacctgccgc gaccgcaacc ggctcgcgct
      601 gcagggcgac attctcggcg ccgtctcgct cggcatccac aacatcctcg cgctggcggg
      661 cgatgatccg aaagcgggcg accagcccga ggcgaaagcg gtctatgacc tcgacagcaa
      721 aggattgctg gcgatggcgc acgcgatgcg ctcgcaaaac aaattaccca gcggaacgaa
      781 gatcgacggc ggcctccacc tcgtgctcgg cgcggccgac gtgccgatcg atccgccgcc
      841 gggctgggag ccgaacagcc ttcgcgccaa ggccgcagcc ggagccgact tcgtgcagac
      901 gcagttctgc atggacatgg gggtggtgcg gcggtatgcc gcgcggctgc tggaggccgg
      961 aatcaagctt ccgatcctca tcggcgtggc gccgatccct tccgcgcgct cggcgcgctg
     1021 gatgcgcgag aagctcttcg gcacgctgat cccggacccg atcatcgagc gcctggagaa
     1081 ggcgcgcgag ccgaagtccg agggcaggaa gatctgcgtc gaaatcctgc gcgagctcgc
     1141 cacgatcccg ggcgtcgcgg gcgcgcatgt catggcgccg atgaacttcg ccgagattcc
     1201 gctcgccatc gaggaatccg gcgtgctgag aaaaaagcgc aaggccgcgg cgtgagcttc
     1261 accgcgagct tcgtcgaggt cgacggctgc aggacgcacc tgcggcgcgg cggcaagggc
     1321 aggccgctgc tctacctgca cggcgcgagc ggcgcgccgg tgatcctgcc attcatggaa
     1381 aagctcgccg cgcgcttcga tgtgctggtg cccgagcatc ccggctacgg gctttcgggc
     1441 gagcccgaat ggctcgagaa catccacgac gtcgcgtatt tttatctcga ttttcttcat
     1501 caactgaacc tgaaagaaac cactcttatt ggaagctcga tgggaggctg gatcgcgatg
     1561 gaaatggcag tacgggatac gagccgcatc agctcgctgg ttctagtcgg cccggcggga
     1621 atcagcgcgc cgggcgtcga gccggccgat attttcctca tgtcccccga agatgtagtc
     1681 aggaatcttt tttttgatca gaagctggcg gaggcgcgcc tcgccgagcc gatgacgccg
     1741 gaagcgctcg acctctcgct caagaaccgg catacaacgg cgcgcctcgc ctgggagccg
     1801 cggctgcacg accctttcct gccgaaatgg ctgcaccgca tcgacgtccc ggtgaagctc
     1861 gtctgggggg cggaggatcg gatccttccg agcgccttta caaaagaatt ctcccgattg
     1921 atgccgaaag cggaaatcca tgtcatcccc gggtgtggcc acctgccgca ggcggagaag
     1981 cccgacgagt tctgcgacat cgtatgcagg tagtcctgtt ccacctcatg ccgtatgccg
     2041 acctcgacct cgaggccggg cgcaagaacg gcacggtgtg ggtgaagctg cccaacaagc
     2101 atttcgaccc ggtgaaaggg cacaagctct acaaccggta cctggacgag ctcgagtacg
     2161 ccgaggcgct cggcttcgac gcggtgtcgg tgaacgagca tcaccagacc gcatacggcc
     2221 tgatgccgtc gccgatcgtc actgcatccg ctcttgcccg aaaaacttcc agggtaaaaa
     2281 tcgcgatact cggaagcgcg ctgccgcttc gatcgcaccc gatgatggtc gccgaggagc
     2341 acgcgatgat cgacgtcatc agcggcgggc gcctgatcag cggcttcgtg cgcggcatcg
     2401 gcgccgaata ccacaccttc ggcgtcaatc cgaccttctc gcacgaccgc ttccacgagg
     2461 cgcacgacct catcatccgc gcctggaccg agcctggccc gttcgcgttt cacggcaagc
     2521 actacaacct gcagtacgtg aacctgtggc cgcgccccta ccagaagccg catccgccgg
     2581 tctggatccc gtcgcagggc tcgaaggaaa ccatcgactg ggcctcgcac ccggagcggc
     2641 gctacaccta cctgcagacg ttcagcccgg tgaaggtggt gggccgctat ctgaagcagt
     2701 accgcgacac tgccaagggc tacggctacg aggcgaagga caaccaatta ggctggtcgc
     2761 tccccgtata tgtttcttct tccgaccaaa aggcaaaaga agaggcgaag gcccatttcg
     2821 aggcgttccg caacgtgttc gttcgcatgc cgctcgagat gctgctgccg ccgggctata
     2881 cctcgcgcga gtcgctgaag aacgtgatga aggcgaaagc gcagatgttc ggcgacgtca
     2941 cgctcgagca ggcgctcgag ctcgggctgt tcgtctgcgg cagcccggag acggtgcgca
     3001 agaacctgga aaatcactgg agcgacatgc gcttcggcaa cctgctggtg tcgtgccagt
     3061 tcggcacgct gcccgccgac ctcacgcgca agaacatgga gctgttcgcg cgcgaggtga
     3121 tgccgcagct aaaggtttta ggaaaataag gcaatcgtga ggggcgcgat attcattggc
     3181 gtccttctcg ccgcagcgtc tttctctgcc tcgccgcaaa cctatcccgc aaaacccgtg
     3241 cgcatcatcg tgccgagcgc gccgggcggc ggctacgatt tcatcggccg cctcgccgcg
     3301 gaaaggtttt cgcaggaatt cggccagggt ttcgtggtgg agaaccgcgt cggcgccggg
     3361 acgctcgtcg gcacgcaggc cgcggcggcc gcgcccgccg acggctacac gcttctcgtc
     3421 ggcggcctcg ccaacatggc gttcaacccg ggcctgcaca aggacctgcg ctacgacccg
     3481 gtggccgatt tcactccgat cgccatggtg ggcgcgttca cttacgcgct cgtcggccgc
     3541 aaggatc
//
LOCUS       DASPXW010000321         4965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_97202, whole genome shotgun sequence.
ACCESSION   DASPXW010000321 DASPXW010000000
VERSION     DASPXW010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97202"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /locus_tag="VEL04_07295"
     CDS             complement(<1..933)
                     /locus_tag="VEL04_07295"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HYT48210.1"
                     /translation="MPLFTYKAIDTRGKSVLGRVEAVNLFDLEQRLARMELDLVSGGP
                     SSQRTRFLGGRVARQDLINFCFHLEQLATAGVPVMEGLVDLRESVENPRFREVVSGLV
                     ESIEGGRSLSQALAEFPEVFGKVFVSLVRSGEQTGKLSEVLKSLTETLKWEDELAAQT
                     KKLMVYPAFVGGIVMLVTFFLMIYLVPQMTGFIRNMGQDVPLQTRLLIAVSNFFVGYW
                     WAIIAAPFVAWAGLKLAVRRNPALQYVIDHYKLAMPLIGPILRKIILSRFASSFAMMY
                     ASGITVLDAIRSCEEILGNRPLEQALHSAGQQIAE"
     gene            complement(944..2653)
                     /locus_tag="VEL04_07300"
     CDS             complement(944..2653)
                     /locus_tag="VEL04_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HYT48211.1"
                     /translation="MNAPANPQAKRHLGQILIDQGILTEDQLRIALLEQTKQHVPVGR
                     LLVQLGFVSEATLRDALSEKLGLQAVDLARIIVDPSALKMVPREMSRRYRIFPVALDR
                     QQKRFIVALSDTNNIVAVDQVRAHLKGEYEIELRLAGDSDIERAIEHYYGHEFSIDGI
                     LKEIETGEVDYTTVTEGDEYAQPVVRLISALLADAVEHSASDIHFEPEQNFLRIRYRI
                     DGVLRQIRSLHKTYWPAMAVRMKVMAKMNIAETRAPQDGRISATLSGRVVDFRVASLP
                     TTHGENLVLRILDRQKGIVPLDKLGLEESQLEMLKRMIARPEGIILVTGPTGSGKTTT
                     LYSILNHINSDGINIMTLEDPVEYPMTLIRQTSVAEASKLDFANGIRAMMRQDPDAIL
                     VGEVRDRETAEMAFRAAMTGHQVYSTLHTNSAVGAFPRLLDIGIVPDILAGNIIGILA
                     QRLVRKLCRTCRQSYDPDVRERRLLGVTPTEPMALFRAVGCEQCDYQGYRGRQSILEL
                     LKIDAGIDELIARRATAREILTAARASGFKTLADDGVRLVRAGTTGLDELMRVVDLTD
                     RMA"
     gene            complement(2721..4049)
                     /locus_tag="VEL04_07305"
     CDS             complement(2721..4049)
                     /locus_tag="VEL04_07305"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYT48212.1"
                     /translation="MSLLLEALKRAEKAKEEAQRRARAEPGAEPGGEPEELQGEGETP
                     AEPKPVLTRDRLPDISQPLEILSDDLVDKTPAAPALEPASAAPAREASARPAPSASRA
                     ERHVSDPDQAGRASARKVFEAKFREPNPRMPFYITMGVLGAFALGTVGYFWVQLRPPP
                     GLVNANPPRQGADVPVATAQSSPAAMFEPRMSNSQPAIPGLPGAVPASPAQPVQAPPP
                     ATLLEQRPQAPRLAPRLAPAPTPEPTEVNVARAAPSVHPKVDAGYSAYLAGDLALARA
                     EYQQVLAEEPGNRDALLGLAALDVRAGRYEAAQAVYQRLLQADPRDAQAQAALIALRG
                     GRVDPLAAESRLKSLLAADPGAHVLNFTLGNQLAQQGRWAEAQQEYFKAFAAEPDNAD
                     FAYNLAVSLDHLHQGKLALEYYQRAVMLAKARGARFDLAAAERRAAQLAR"
     gene            complement(4051..>4965)
                     /locus_tag="VEL04_07310"
     CDS             complement(4051..>4965)
                     /locus_tag="VEL04_07310"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HYT48213.1"
                     /translation="QVLIEATVTEVQLRNEYQRGIDWQRLRSPGISFGQPTITPSTSA
                     TPGFTPSALLPFIGFVSGGGGFNATLKLLEQFGDVKVLSSPKLSVMNNQTAILRVTRD
                     IIYFTVTPSTQPITIAAGGGGATIQSSFTTTPNVAAEGFMMAVLPQINESDAVVLNVR
                     PTIRRRVDSVADPNPALQATPSNPNAIPNSIPVFETREFDSILRLQSGQVGVLAGLMQ
                     DIAENTDTGIPGIRSIPLIGDILSNRAQLSRKTELVIFLRATVIRDPSVEGDFRRFRD
                     QVPGEDFFLKPNPARVAPPLGPSGEPLD"
BASE COUNT          786 a         1603 c         1702 g          874 t
ORIGIN      
        1 ttcggcgatc tgctgcccgg cgctgtgcag cgcctgctcg agcggtctgt tgccgaggat
       61 ttcctcgcag ctgcggatcg cgtcgagcac ggtgatgccc gaggcgtaca tcatggcgaa
      121 gctcgacgca aagcgcgaca ggatgatctt gcgcaggatc ggcccgatga gcggcatcgc
      181 gagcttgtag tggtcgatca catactggag cgccgggttc ctgcgcaccg cgagcttgag
      241 ccccgcccaa gcgacgaaag gcgcggcaat gatcgcccac cagtagccga cgaagaagtt
      301 cgacaccgcg atcaataacc gcgtctgcag gggaacgtct tgccccatgt tgcggatgaa
      361 gccggtcatc tgcggcacga gatagatcat caggaagaac gtcaccagca tcacgatgcc
      421 gccgacgaag gcggggtaca ccatcagctt tttggtctgg gccgcgagct cgtcctccca
      481 cttcagcgtc tcggtgaggc ttttcagcac ctcggagagc tttccggtct gctcccccga
      541 gcgcaccagg ctcacgaaca ccttgccgaa gacctccggg aactcggcaa gcgcctgcga
      601 caggctcctg ccgccttcga tcgactcgac cagccccgac accacttcgc gaaagcgtgg
      661 attctcgacg ctctctcgaa ggtcgaccag cccttccatc accggcacgc cggcggtggc
      721 gagctgctcg aggtggaagc agaagttgat cagatcctgg cgcgccaccc gaccgccgag
      781 aaagcgcgtg cgctggctcg aaggccctcc gctgaccagg tcgagctcca tgcgcgcgag
      841 acgttgctcg aggtcgaaca ggttgaccgc ctcgacccgg ccgagcacgc tcttgccgcg
      901 cgtgtcgatg gccttgtagg tgaacagcgg catcgacgcg cccttacgcc atgcggtcgg
      961 tgagatcgac cacgcgcatg agctcatcga gtccggtggt tcccgcgcgc accagccgca
     1021 cgccgtcgtc ggcgagcgtc ttgaagccgc ttgcccgcgc ggccgtgagg atctccctcg
     1081 cggtcgcccg ccttgcgatc aactcgtcga tgcccgcgtc gattttcagc agctcgagga
     1141 tcgactgcct gccacggtag ccctggtaat cgcactgctc gcagccgacc gcgcggaaca
     1201 gcgccatggg ctcggtgggc gtgacgccga gcaggcgccg ttcgcgcacg tcggggtcat
     1261 aggattgccg gcaggtgcgg cacagttttc tcaccaggcg ctgcgccagg atgccgatga
     1321 tgttgccggc gaggatgtcg ggcacgatgc cgatgtcgag cagccgcggg aaagccccga
     1381 ctgccgagtt ggtgtgcagc gtcgagtaca cctggtggcc ggtcatggcg gcgcgaaacg
     1441 ccatctcggc ggtctcgcgg tcgcgcacct cgcccaccag gatcgcgtcg gggtcctggc
     1501 gcatcatcgc gcggatgccg ttggcgaagt cgagcttgga cgcctccgcc accgaggtct
     1561 ggcggatgag cgtcatcgga tactcgaccg ggtcctccag cgtcatgatg ttgatgccgt
     1621 cggagttgat gtggttgagg atcgagtaga gcgtggtggt cttgccgctg ccggtcgggc
     1681 cggtcaccag aatgatgccc tcggggcgcg cgatcatgcg cttcagcatc tcgagctgcg
     1741 actcctcgag gccgagcttg tcgagcggca cgatgccttt ctggcggtcg aggatccgca
     1801 gcaccaggtt ctcgccgtgg gtggtcggca ggctggcgac cctgaaatcc accacccggc
     1861 ccgacagcgt ggccgagatg cggccgtcct gcggcgcacg cgtctcggcg atgttcattt
     1921 tcgccatgac tttcatgcgc accgccatgg ccggccagta ggtcttgtgc aggctgcgga
     1981 tctggcgcag cacgccgtcg atgcggtagc ggatgcgcag gaagttctgc tcgggctcga
     2041 agtggatgtc ggaggcgctg tgctcgaccg cgtctgcgag cagcgccgag atcagccgca
     2101 ccacgggctg cgcgtattcg tccccctcgg tgaccgtggt gtagtcgact tcgccggtct
     2161 cgatctcctt caggatgccg tcgatcgaga actcgtggcc gtagtaatgc tcgatcgcgc
     2221 gctcgatgtc ggagtcgccg gcaagccgca gctcgatctc gtattcgccc ttgaggtggg
     2281 cgcgcacctg gtcgaccgcg acgatgttgt tggtgtccga gagcgcgacg atgaacctct
     2341 tctgctgccg gtcgagcgcc accgggaaga tgcggtagcg ccgcgacatc tcgcgcggca
     2401 ccatcttcag cgcagacggg tcgacgatga tgcgcgcgag atccaccgcc tgcaggccga
     2461 gcttctccga gagcgcgtcg cgcagcgtcg cctcggacac gaagccgagc tgcaccagca
     2521 gccgcccgac cgggacatgc tgcttcgtct gctcgagcag cgcgatgcgc agctggtcct
     2581 cggtgaggat cccctggtcg atcaggatct ggccgagatg gcgcttggcc tgcggattgg
     2641 cgggtgcatt catgtggacg agatgataac cgcggctctc tcccgggcgg tgtcaactat
     2701 tccacggctg tcaggaactg tcagcgggcg agctgcgcgg cgcgcctttc ggcggcagcc
     2761 agatcgaagc gcgcgccgcg cgccttggcg agcatgacgg cgcgctggta atactcgagc
     2821 gcgagctttc cctggtgcag gtgatcgagg ctgacggcga ggttgtaggc gaagtcggca
     2881 ttgtcgggct cggcagcaaa ggccttgaag tactcctgct gcgcttcggc ccagcggccc
     2941 tgctgcgcga gctggttgcc gagcgtgaaa ttgagcacat gcgcgccggg gtcggcggcg
     3001 agcagcgact tcagccggct ctcggccgcg agcgggtcga cacgcccgcc gcgcagcgca
     3061 atgagggccg cctgcgcctg cgcatcgcgc gggtccgcct gcagcagccg ctggtagacg
     3121 gcttgcgccg cttcgtagcg gccggcgcgc acgtcgagcg ccgcgaggcc gagcagcgcg
     3181 tcgcggttcc ccggctcctc ggcgagcacc tgctggtatt cggcgcgcgc tagggcgaga
     3241 tcgccggcga ggtacgcgga atatccagcg tccaccttcg gatgcacgct tggcgcagcg
     3301 cgggctacgt tcacttccgt cggctccggc gtcggcgcgg gggccaagcg cggtgcaagg
     3361 cgcggagctt gcggcctctg ctccaggagc gtcgccggcg gcggcgcctg aacgggttgt
     3421 gccggcgatg ccgggacggc tcccggcaac ccgggaatcg ccggctgcga gttgctcatg
     3481 cggggctcaa acatcgcagc agggctgctt tgcgccgttg ccactggcac atcggcgccc
     3541 tggcgcggcg gattggcatt cacgagcccc ggtggcggcc gcaactggac ccagaaatag
     3601 ccgaccgtcc cgagcgcgaa ggcgccgagc acacccatcg tgatgtagaa gggcatgcgc
     3661 ggattgggtt ccctgaactt cgcctcgaag accttgcgtg ccgaggcgcg gcccgcctgg
     3721 tccggatcgc tcacgtgacg ctccgcgcgc gacgcagacg gcgcagggcg cgcgctcgcc
     3781 tcgcgtgcgg gcgcggcgct ggccggctcg agcgctggcg cagcgggcgt cttgtcgacc
     3841 agatcgtcgc tcaggatttc gagcggctgc gagatatcgg gcagcctgtc gcgggtgagg
     3901 accggcttgg gctcggcagg cgtttcgcct tcgccttgca gctcttcggg ctcgccgccg
     3961 ggctcggcgc caggctcggc gcgggcccgg cgctgggcct cttcctttgc cttctcggct
     4021 ctcttgagcg cctcgagcag caggctcatg tcaatccagc ggctcgccag aagggccgag
     4081 cgggggcgcg acgcgggctg gattgggctt caggaagaaa tcctctcccg gcacctgatc
     4141 acggaagcgc cgaaaatccc cttccacgct cggatcgcga atcaccgtgg cgcgcagaaa
     4201 aatgaccagc tccgttttcc tgctgagctg tgcgcggttg gagagaatat cgccgatgag
     4261 cggaatgctg cggatgcctg gaatgcccgt gtctgtgttt tccgcgatgt cctgcatcag
     4321 tccagcgaga acgcctactt ggccgctctg caggcgcagg atggaatcga attcgcgtgt
     4381 ctcaaaaacc ggaatggagt tcggaatggc gttcggattg gagggggtgg cttgcaaggc
     4441 cggattggga tccgccaccg agtcaacccg cctgcgaatc gttggccgga cgttcagcac
     4501 cacggcatcg ctttcattga tttgcggcag caccgccatc atgaagccct cagcggcaac
     4561 gttcggcgtt gtggtgaacg aggactgaat agtggcgccc cctccgcctg ccgcgatggt
     4621 gatcggctgc gtcgaaggcg tgacggtgaa gtaaatgatg tcgcgcgtca cccgcaggat
     4681 cgcggtctga ttgttcatca cgctgagctt cgggctggac aggactttca cgtccccgaa
     4741 ctgctcgagc agcttgagag tggcattgaa gccgccaccg cccgagacaa agccgatgaa
     4801 gggcagcaag gctgaggggg taaaaccggg tgtcgccgag gtgctcggcg ttatcgtcgg
     4861 ctgaccgaac gatatgcctg gcgagcgcag acgctgccag tcgatgccac gctggtactc
     4921 gttgcgcagc tgcacctcgg tgaccgtcgc ctcgatcagc acctg
//
LOCUS       DASPXW010000322         2363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_97383, whole genome shotgun sequence.
ACCESSION   DASPXW010000322 DASPXW010000000
VERSION     DASPXW010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97383"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VEL04_07315"
     CDS             complement(<1..488)
                     /locus_tag="VEL04_07315"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HYT48214.1"
                     /translation="MRDKPFLQPDLAVGEALRAVAGDILAAARATVEDPARSDAGAVH
                     DFRREMKRWRALLRLLEPFLGADGQRLRTDARDHARALGGARDAQSALDAFEDIAGHG
                     LDLSQNSLEAVRGRIEELKQAAETTTLTADMRLRITAALDQALAAVEVWPLHLLSFQD
                     VA"
     gene            632..708
                     /locus_tag="VEL04_07320"
     tRNA            632..708
                     /locus_tag="VEL04_07320"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:666..668,aa:Met,seq:cat)
     gene            977..2140
                     /locus_tag="VEL04_07325"
     CDS             977..2140
                     /locus_tag="VEL04_07325"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HYT48215.1"
                     /translation="MATLAERDRRILTTHAGSLPRRSSLSALLFARMSKQPFDAGALA
                     RETTEAVAEIVRKQRALGIDIVSDGEQSKTSFQHYIADRLSGLESIVPKAGERRTREN
                     IAFPAFYKGGAHSGSAQARFACTGPIKYTGHKQLAEDLSNLKQALGGQDPVGAFVPSV
                     SPSSCAGTMENRYYKSDDEHVMAVAEAMREEYEGIVAAGFQVQIDDPRLAMHYMLTPG
                     ESVEDTRGWARRRIEVLNHALRNIPPERVRHHTCYGINMGPRTSDLEMKHLADLIITI
                     RAGYYSFEMANPRHEHEWRVWEHVKLPDDKVLVPGCITHASVIVEHPELVAERIVRLA
                     GIVGRERVVASSDCGFASTLAPNQPPEVEPEIVWAKFESLVAGARLATKALWT"
BASE COUNT          417 a          805 c          785 g          356 t
ORIGIN      
        1 ttggcgacgt cctggaacga gaggagatgc agcggccaga cctcgaccgc ggccagcgcc
       61 tggtcgagcg ccgcggtgat gcgcagccgc atgtcggcgg tcagcgtcgt ggtctcggcc
      121 gcctgcttga gctcctcgat ccggccgcgc accgcttcga gcgaattctg cgacaggtcg
      181 agcccgtggc cggcgatatc ctcgaacgcg tcgagcgccg attgcgcgtc gcgggcgccg
      241 ccgagcgcgc gggcgtggtc gcgagcgtcc gtgcgcaggc gctggccgtc ggcgccgagg
      301 aacggttcga gcaggcgcag caaggcgcgc cagcgcttca tctcgcggcg gaagtcatgg
      361 accgccccgg cgtccgagcg cgccgggtcc tcgacggtag cccgcgcggc ggcgaggatg
      421 tccccggcga cggcgcgcaa agcctcaccc accgccaagt ccggttggag gaagggcttg
      481 tcgcgcatcg ggaccgattt attgacgaag ttccgtgacg ttttgacgaa agcccgaatt
      541 ttggcccttt tcaaggggtg ccacgatcgt tacataatcc ctgcgccgcg ggatcgatcc
      601 cgccgccttc tcaggcaacg catcggattg acgcggggtg gagcagcccg gtagctcgtc
      661 aggctcataa cctgaaggtc gtaggttcaa atcctacccc cgcaaccatc cgccccatca
      721 tagatggggc aagcgaggac cctagcctcg gcaggcgcag caaggcggcc aggcccctcc
      781 caatccccca ggccccgcag ggcaagcgaa cgctccggcg gcagtccgcc tctagcgaac
      841 tggcgcagtc atcaatcaga tttgtgcgcc ataaaatttt atcggacgta agcccgggtg
      901 ataaaagcca agaaccaaga tggtacggaa gtgcgacaga cagggctctt ccgcagcaaa
      961 gggaccgcgc gcggccatgg ccacactcgc agaacgcgat cgacgcatcc tcaccaccca
     1021 cgccggaagc ctgccgcggc ggtcctcctt gtcggccctg ctgttcgcgc gcatgagcaa
     1081 gcagccgttc gatgccggcg cgctggcgcg ggaaacgact gaggcggtgg ccgagatcgt
     1141 gcgcaagcag cgcgcactcg ggatcgacat cgtcagcgac ggcgagcagt cgaagacgag
     1201 cttccagcac tacatcgccg accgcctgtc tggcctggaa tcgattgtcc cgaaagccgg
     1261 cgagcggcgc acccgcgaga acatcgcctt cccggcgttc tacaaaggcg gcgcccactc
     1321 cggctccgcg caggccaggt tcgcctgcac gggaccgatc aagtacaccg gccacaagca
     1381 gctcgccgag gatttgtcga acctgaagca ggcgctcggc gggcaggacc cggtcggggc
     1441 gttcgtcccg tcggtgtcgc cgtcgagctg cgccgggacg atggagaacc gctactacaa
     1501 gagcgacgac gagcatgtca tggcagtcgc cgaagcgatg cgcgaggagt acgagggcat
     1561 cgtggccgcc ggcttccagg tgcagatcga cgatccgcgg ctcgccatgc actacatgct
     1621 gacgcccggc gagtcggtcg aggacacccg cggctgggcg cgccgccgca tcgaggtgct
     1681 caaccacgcg ctgcgcaata ttccgcccga gcgcgtccgg caccacacct gctacggcat
     1741 caacatgggg ccgcgcacca gcgatctcga gatgaagcat ctcgccgatc tcatcatcac
     1801 catccgcgcc ggctactatt cgttcgagat ggccaatccg cgccacgagc acgaatggcg
     1861 ggtgtgggaa cacgtgaagc tgcccgatga caaggtgctg gtgccgggct gcatcaccca
     1921 tgcctcggtc atcgtcgagc atccggagct ggtcgccgag cggatcgtgc ggctggccgg
     1981 gatcgtcggg cgcgagcgcg tcgtcgcgag cagcgattgc ggctttgcct ccacgctggc
     2041 gcccaaccag ccgccggagg tcgagccgga gattgtttgg gcgaaattcg aaagcctggt
     2101 ggcgggggcg cgcctcgcca ccaaggcgct ctggacgtag ccagcgcctc caccctctcc
     2161 gttcgtcccc gcgcaggcgg ggacccagaa tcgcgaggcc agcgttcgtg gctcctgggt
     2221 ccccgctttc gcggggacga acggagcgcg ctgatccgtt cgaaagggat ccgctctaat
     2281 gcgcaagcac ctcatcgatc caggccgcca gcgtatcgag cacctcgccg cggacctgcg
     2341 ggtccttgcg gccggtctta gcc
//
LOCUS       DASPXW010000323         8052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_346631, whole genome shotgun sequence.
ACCESSION   DASPXW010000323 DASPXW010000000
VERSION     DASPXW010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8052
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346631"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VEL04_07330"
     CDS             complement(<1..527)
                     /locus_tag="VEL04_07330"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HYT48216.1"
                     /translation="MLPSVFISHGSPMHTLEPGAAGEVWAALGRRFPRPRAVLIASAH
                     WETNIPMLSGGEKPETVHDFYNFPQPLYRLRYPAPGAPEVAQRAQALLKDAGFTAGID
                     GCRGLDHGAWSPLLYMYPKADIPVVQISVQPAFGPRHHVEVGRSLKKLSEEGVLIIGS
                     GHMTHNLRDWSRGSG"
     gene            complement(533..1150)
                     /gene="gstA"
                     /locus_tag="VEL04_07335"
     CDS             complement(533..1150)
                     /gene="gstA"
                     /locus_tag="VEL04_07335"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HYT48217.1"
                     /translation="MKLYYAPGACSLSPHIVSRELGLPIELKKVNTKDKTVEGGGDYW
                     KVNGRGYVPALELDNGQVLTEGPAIVQYLADQKPESGLAPKAGTLERYRLQEWLNFLT
                     SEIHKQFSPLFKPNTPDEYKKIAKENLATRFDWLEKQLAGKDYLMGKSFSVADAYLFV
                     LLGWTKPTQIDLSRWPNLAAFHARVGARPKVQEALQVEGLLKKAA"
     gene            complement(1161..2039)
                     /locus_tag="VEL04_07340"
     CDS             complement(1161..2039)
                     /locus_tag="VEL04_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HYT48218.1"
                     /translation="MKNVLSIHREGESHWVGDGFPVHTVLDYDRHPELSPFILLDHAG
                     PAKFAPTNKPRGVGWHPHRGFETVTVVFDGEVDHQDTAGNGGRISQGDVQWMTAGSGL
                     LHKEMHSAEFTRRGGPFHALQLWVNLPAKSKMTPPRYQTLLARDIPSVALPEEAGTVR
                     VIAGEFGGAKGPAETFTPVNLLDMRLRANHRARLSLRDGFSAGLYVVKGRIAVNGEAA
                     SEGELVVLDRKGDEVTIEAPADATVFVMNGAPIDEPVAGYGPFVMNTQDQIRQAFADY
                     QRGRLGRIPSPTVSTS"
     gene            2126..3034
                     /locus_tag="VEL04_07345"
     CDS             2126..3034
                     /locus_tag="VEL04_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HYT48219.1"
                     /translation="MDRLTAIQVFAQVVESGSFAKAAARLGLSTSATSRHVAELEAHL
                     QTRLLNRTTRRVSLTESGRAFYERTVQLLADLEEAEREASRAAVVPRGVIRLTTAVNF
                     GVRHLAPAIAEFLAKHGELRFDVSLSDRIVDLVEEGFDLAIRIGSAGPHNVVARKLGE
                     TRAMLCASPAYLAAHGTPQVPEDLERHNCFTYEYVSPRNVWHFRDPSGRERPVRVSGN
                     LHSNNGDLAAEVAARGGGIVFEPAFIVGPEVRAGRLVPLLQDFVPPPVPIFAVYPSRK
                     QLSAKVRVFVDFLVERFAEAQDFSAA"
     gene            3031..4119
                     /locus_tag="VEL04_07350"
     CDS             3031..4119
                     /locus_tag="VEL04_07350"
                     /inference="COORDINATES: protein motif:HMM:NF014324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HYT48220.1"
                     /translation="MKAIVLALSLLFAAGAAAQVPDWVLVSPTGRAEAGASFELLLLG
                     PEGATLPDEIALRMKIGVSELDLRMQARSPQENGRRAYVGTMPAAAAGAAALELADPP
                     SNVVVVLVTRRDAVQALSGQPAGGEEPLMSEDEPMYFVVGARGGASARFQLSFKYRLF
                     DYSSGFGSEQPWLAGIYFGYTQNSLWDLSAESRPFRDTSYRPSLFWKWQRTDERTWID
                     AARLGIEHESNGRDAPNSRSINILFVRPEWRWRTAGLGNFEFTPKIYAYLEKDDNPDI
                     QRYRGYVDWRARHDSGGDWIATGIARVGTSGHGSLLVDLSRRTRGLKIGPLSGYLHAQ
                     YFNGYGEDILDYNVRRKAQLRLGFAIVP"
     gene            complement(4131..4700)
                     /locus_tag="VEL04_07355"
     CDS             complement(4131..4700)
                     /locus_tag="VEL04_07355"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HYT48221.1"
                     /translation="MTPRAITIRLAEPRDAQAVAMMSRDFVESGLGWKYDAARVLRAI
                     RDRETLAVVACESGKSAASSNTTTARGALTGFAIMELGDERAHLVLLAVRPSHRRLGI
                     GQHLLEWLLESARIAGMASIHLELRAGNDAARRFYRAMGFYETVLVPGYYRSGEGRKE
                     GALRMLRVLRVPGPVPYTWRPPRTPEDKA"
     gene            4747..5946
                     /locus_tag="VEL04_07360"
     CDS             4747..5946
                     /locus_tag="VEL04_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HYT48222.1"
                     /translation="MTITPELVDEVKSLSAEDAANKIAERSGADIVATLMALRPAFAQ
                     DVLGALPAESRERTLAAAPGEVSRQWQRNALYPAGSTGRMMEPVIAAYPPEHTVGETI
                     ESLRELVKTAFITYIYVRDRDERLLGIVTMRDLLFSEHAKTLGEVMLRAVFALQAVTP
                     LIEAMKQVLDRHYPVYPVVDADGRLRGLVRGQEMFEAQAIEISLQAGSMVGLEKEERL
                     ATHWWSSLKMRHPWLQLNLLTAFLAAAVVGVFQDTIDRLVILALFLPVLAGQSGNTGC
                     QALAVTLRGMVLGELKPGSERPLISKEAWLGCLNGAGVGLMAALGMYLTATGQHQTQP
                     VMLAFVVLLAMIGSCIASGLAGAMVPLTLKRLGFDPATASSIFLTTATDVVSMGMLLA
                     LATIMVR"
     gene            6033..6467
                     /locus_tag="VEL04_07365"
     CDS             6033..6467
                     /locus_tag="VEL04_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007585445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HYT48223.1"
                     /translation="MQVKEILRVKGSRLLSIEPGGRAVDAVNTMAKENLGSLVVMEQG
                     RMVGMLTFHELLGALASRGGALGELRVTDIMVRDPVTAAPEMEVNDLRRTMLESGARY
                     LPVMQQGKLLGVISFRDVAKAVLEEQDFENKMLKGYIKNWPA"
     gene            6595..7689
                     /locus_tag="VEL04_07370"
     CDS             6595..7689
                     /locus_tag="VEL04_07370"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYT48224.1"
                     /translation="MRGAWEGIDAAQAWDSHAHLVGSGDSDSGIFVNPRMESLLNPGE
                     YARRLFFLNAGCAHSAPGNVDRIYVERMRNLVDGMQGEGKRGVKLLLYAFDRVHDERG
                     APDMERSAFYVPDSYARDTAKRHPGYFEWAASIHPYRGDAADALRKAKTEGARAVKWL
                     PAAMGIDPASPRCDAFYEVMAKLDLPLISHAGEERAVLGHDSHDYGNPLRLRRALDAG
                     VRVVIAHCASLGKDRDLDQGADGPYVDSFALFTRVFEKYEKHCYGDISAMTQVNRAGP
                     ALARVIEETSWHARLLNGSDYPLPGVMPIFSVDYLISLGMLAPKAAPVLTEIRLHNPL
                     LFDFVVKRHLRSNGKALSASVFETRMFFMR"
     gene            7697..>8052
                     /locus_tag="VEL04_07375"
     CDS             7697..>8052
                     /locus_tag="VEL04_07375"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HYT48225.1"
                     /translation="MSGNSFGVLYCVTSFGESHGPAYGGVVDGCPPGLELSAADIQRE
                     LDRRKPGSSRHVTQRRESDAVEILSGVYQGRTTGTAIGFLVRNEDQRPKDYSAIADKF
                     RPGHADYTYWQKYGVR"
BASE COUNT         1296 a         2621 c         2724 g         1411 t
ORIGIN      
        1 ttgcccgagc cacgcgacca gtcgcgcagg ttgtgcgtca tgtggccgga accgatgatg
       61 agcacgcctt cttccgataa tttcttcagt gaacgcccta cttcgacgtg atgccgtggg
      121 ccgaaggcgg gctgtaccga gatctgcacc accgggatat cagccttcgg gtacatgtaa
      181 agaagcggcg accaggcgcc gtgatccagg ccgcggcagc cgtcgatacc ggcggtgaag
      241 cccgcgtcct tcaggagcgc ctgcgcgcgc tgggcaactt ctggtgcgcc gggagccggg
      301 tagcgcaggc ggtagagcgg ttgcgggaag ttgtagaagt cgtggaccgt ttcgggcttc
      361 tcgcctccgc tgagcatcgg aatattggtc tcccagtgcg cagaggcgat caggacggcg
      421 cgcggcctgg gaaagcgcct gccgagcgcc gcccagacct cgccggccgc gcccggctcg
      481 agcgtgtgca tcggcgagcc gtgggagatg aagacgctcg gcagcacgcc ggtcaggcag
      541 cttttttgag gagaccctct acctgaaggg cttcctgcac cttaggccgc gccccgacgc
      601 gggcgtgaaa cgcggcgaga ttcggccacc tggaaagatc gatctgcgtc ggcttggtcc
      661 agccgagcag cacgaacaga tacgcgtcgg cgacgctgaa cgacttaccc atcaggtagt
      721 ccttgccggc gagctgcttc tccagccagt cgaagcgcgt ggccaggttc tccttggcga
      781 tcttcttgta ctcgtcgggc gtgttcggct tgaagagcgg cgagaactgc ttgtggatct
      841 cggaggtgag gaaattgagc cattcctgca gccggtagcg ctccaacgtg cccgctttcg
      901 gcgcgaggcc ggattcgggc ttctggtcgg cgaggtattg gacgatcgcc ggaccttcgg
      961 tgagcacctg gccgttgtcg agctcgagcg cgggcacgta gccccggccg ttcactttcc
     1021 agtagtcgcc gccgccttcg acggtcttgt ccttggtgtt gaccttcttc agctcgatgg
     1081 gcagccccag ctcgcgcgac acgatgtgcg gtgaaagcga gcaggcgccg ggggcgtaat
     1141 acagtttcat tcgtttctcc tcaggaggtt gacacggtgg gtgaaggaat ccgccccagc
     1201 cggccgcgct ggtagtcggc aaaggcctgc cggatctggt cttgcgtgtt catgacgaag
     1261 gggccgtagc ccgcgaccgg ttcgtcgatc ggcgcgccgt tcatgacgaa aacggtggcg
     1321 tctgcgggcg cctcgatagt cacttcgtcg cccttgcggt cgagcacgac gagctcgcct
     1381 tcactcgccg cctcgccgtt cacggcgatc cggcctttca cgacgtagag ccccgcgctg
     1441 aagccgtcgc gcaggctcag gcgtgcgcga tggtttgcgc gcaggcgcat gtcgagcagg
     1501 tttaccggcg tgaaagtctc cgcgggacct ttcgcgccac cgaactcgcc ggcgatgacg
     1561 cgcactgtgc cggcttcttc aggaagggcg accgagggaa tgtcccgcgc gagcagcgtt
     1621 tggtagcgcg gcggcgtcat cttcgatttc gccggcaggt tcacccacag ctgcagcgcg
     1681 tggaaggggc cgccgcggcg cgtgaactcg gcactgtgca tttccttgtg cagcaagccg
     1741 gagcccgccg tcatccactg cacgtcgccc tggctgatgc ggccgccatt gcccgccgtg
     1801 tcctggtgat cgacctcgcc atcgaagacg acggtcacgg tctcgaagcc gcggtgcgga
     1861 tgccagccca cgccgcgcgg cttgttagtg ggtgcgaact tcgcaggacc ggcgtgatcg
     1921 agaaggatga atggcgagag ctcgggatgc cggtcgtagt cgagcaccgt gtgcaccggg
     1981 aagccgtctc ctacccagtg gctttcgcct tcgcgatgga tactgagaac gtttttcatg
     2041 cctgacagta tgggtatgca tttcccaggg ataaagatgt tcttatagga aatataatcc
     2101 cgaataaagg gacaatatag ccatcgtgga ccgcctgact gccatccaag tcttcgccca
     2161 agtcgttgaa tcggggagct ttgccaaggc cgcggcgcgt ttggggctgt cgacctcggc
     2221 cacgtcgcgg catgtcgccg agctcgaggc gcatctgcag acccggctcc tcaatcgcac
     2281 cacgcgccgc gtgagcctca ccgagagtgg gcgcgcattc tacgagcgca ccgtgcagct
     2341 gcttgccgac ctcgaggaag ccgagcgcga ggcctcgcgc gcggcggtcg tgccgcgcgg
     2401 cgtgatcagg ctcaccaccg cggtgaactt cggcgtacgc catctcgccc cggcgatcgc
     2461 cgagttcctc gcgaagcatg gagagctgcg cttcgatgtc tcgctctccg accgcatcgt
     2521 cgacctggtc gaggagggtt tcgacctcgc catccgcatc ggcagcgccg gcccccacaa
     2581 cgtcgtcgcg cgcaagcttg gcgagacccg cgccatgctt tgcgcctcgc cggcgtatct
     2641 cgccgcccac ggcacgccgc aggtgcccga ggacctcgag cgccacaact gcttcaccta
     2701 cgaatacgtt tcgccgcgca atgtctggca cttccgcgat ccttccggcc gcgagcgtcc
     2761 ggtgcgggtg agcggcaacc tgcattcgaa caacggcgat ctggccgccg aggttgccgc
     2821 gcgcggcggc gggatcgtgt tcgagcccgc cttcatcgtc ggccccgagg tgcgggccgg
     2881 tagactggta ccgctgttgc aggatttcgt gccgcctccg gtgcccatct tcgctgtcta
     2941 tccgagtcgc aagcagcttt cagccaaggt gcgcgtgttc gtcgacttcc tcgtcgagcg
     3001 ctttgccgag gcacaggact tcagcgcagc gtgaaagcca tcgttcttgc cctgagcctg
     3061 ttgtttgcgg ccggcgccgc ggcgcaggtg ccggactggg tcctggtctc gccgaccggc
     3121 cgcgccgagg cgggggcgag cttcgagctg ctgctgctcg gtcccgaagg cgcgacgctg
     3181 cccgacgaga tcgcgctgcg catgaaaatc ggcgtctcgg agctcgacct gcgcatgcag
     3241 gcgcgctcgc cgcaggagaa cgggcgccgc gcatacgtcg gcaccatgcc ggcggcggct
     3301 gccggcgcgg cggcgctcga gctcgccgat ccgccctcga acgtggtcgt ggtcctagtc
     3361 acccggcgcg acgcggtgca ggcgctttcc gggcagccgg ccggcggcga ggagccgctg
     3421 atgtcggagg atgagccgat gtacttcgtg gtcggcgcgc gcggcggcgc gagcgcgcgc
     3481 ttccagctgt cgttcaagta ccgcctgttc gactactcga gcggcttcgg cagcgagcag
     3541 ccctggctcg cagggatcta cttcggctac acgcagaatt cgctctggga cctgtcggcc
     3601 gagtccagac ccttccgcga caccagctac cggccgtcgc tgttctggaa atggcagcgc
     3661 accgacgagc gcacctggat cgacgccgcg cgcctcggca tcgagcacga gtcgaacggg
     3721 cgcgacgctc caaactcgcg ctcgatcaac attctgttcg tgcggcccga atggcgctgg
     3781 cgcacggcag gactcggcaa tttcgagttc acgccgaaga tttacgccta cctcgaaaag
     3841 gacgacaacc cggacatcca gcgctaccga ggctacgtcg actggcgcgc gcgccatgac
     3901 tcgggcggcg actggatcgc gaccggcatc gcgcgggtcg gcacctcggg ccacggcagc
     3961 ctgctcgtcg atctgtcgcg gcgcacgcgc gggctgaaaa tcgggccgct gagcggctac
     4021 ctgcacgcgc agtacttcaa cggctacggc gaggacatcc tggactacaa cgtgcggcgc
     4081 aaggcgcagc tgcgcctggg gtttgcgatc gtgccgtaaa agcgtacctt ttaggccttg
     4141 tcttctgggg tgcgcggggg gcgccaggtg tagggcaccg gcccgggaac gcgcagcacg
     4201 cgcagcatgc gcagcgcgcc ttccttgcgg ccttcgccgc tgcggtagta gccggggacg
     4261 agcaccgtct cgtagaagcc catcgcccga tagaagcggc gcgcggcgtc gttcccggcg
     4321 cgcagctcga ggtggatgct ggccattccc gcaatgcgcg ccgactcgag cagccattcg
     4381 agcaggtgct ggccgatgcc gagccggcgg tgcgagggcc gcactgcgag cagcacgaga
     4441 tgcgcgcgct cgtcgcccaa ttccatgatc gcgaacccgg tcagcgcgcc tcgcgcggta
     4501 gtggtattgg aggacgcggc gctcttgccg ctttcgcacg ccaccactgc gagcgtctcg
     4561 cggtcgcgga tcgcgcgcaa cacgcgcgcc gcgtcgtact tccatcccag ccctgactcg
     4621 acgaagtcgc gcgacatcat cgccacggcc tgggcgtcgc gcggctcggc gaggcggatg
     4681 gtgatggcgc ggggcgtcat ggcgcaattg tgccatgatc tgatctgggc ttatagggag
     4741 ggaaatgtga ccatcacgcc tgagctggtg gacgaagtca agtcgctctc ggccgaagac
     4801 gctgcaaata aaatcgccga gcgcagcggc gcggacatcg tcgccacgct catggcgctg
     4861 cgaccggcgt tcgcgcagga cgtgctcggc gcgctgccag ccgaatcgcg cgagcgcacg
     4921 ctcgccgcgg cgcccggcga agtctcgcgc cagtggcagc gcaacgcgct ctacccggcg
     4981 ggctcgaccg gccgcatgat ggagccggtg atcgcggcgt atccgcccga gcacacggtg
     5041 ggcgagacca tcgaatcgct gcgcgagctg gtgaagacgg cgttcatcac ctatatctac
     5101 gtccgcgacc gggacgagcg gctgctcggc atcgtgacga tgcgcgacct gctgttcagc
     5161 gagcatgcga agaccctggg cgaggtgatg ctgcgcgcgg tgttcgcgct gcaggccgtc
     5221 actcctctga ttgaggcgat gaagcaggtc ctcgaccggc actatcccgt ctacccggtg
     5281 gtcgatgccg acgggcgcct gcgcggcctg gtgcgcgggc aggagatgtt cgaggcgcag
     5341 gcgatcgaga tctcgctgca ggcgggcagc atggtcggcc tggagaagga ggagcgtctc
     5401 gccacccact ggtggtcgag cctgaagatg cgccatccat ggttacagct caacctgctc
     5461 accgcgtttc tcgccgcggc ggtggtcgga gtattccagg acacgatcga tcgcctcgtg
     5521 atcctcgcgc tgttcctgcc ggtgctcgcc gggcagtcgg gcaataccgg ctgtcaggcg
     5581 ctcgcggtga cgctgcgcgg catggtgctc ggcgagctca agccgggctc cgagcggccc
     5641 ctcatcagca aggaagcctg gctgggctgc ctcaatggcg cgggcgtcgg gctgatggcg
     5701 gcgctcggca tgtacctcac cgcgaccggg cagcatcaga cccagccggt gatgctcgcc
     5761 ttcgtcgtgc ttctcgccat gattggctcg tgcatcgcga gcggcctcgc cggcgccatg
     5821 gtgccgctca cgctcaagcg cctgggcttc gacccggcga ccgcctcgag catcttcctc
     5881 accacggcga ccgatgtcgt gagcatgggc atgctgctcg ccctcgccac gatcatggtg
     5941 cgctagacgt ctcagcgcga gcggctgcgc attcccgagc gccgccgttt cgactagact
     6001 tgctccggta gcgccgcgat ggaggtcgcg ccatgcaagt caaggagatc ttgcgggtca
     6061 agggcagccg gctcctcagc atcgagcccg gcggccgggc ggtagacgcc gtcaacacca
     6121 tggcgaagga gaacctcggc tccctagtgg tgatggagca ggggcggatg gtcggcatgc
     6181 tgacctttca cgagttgctg ggcgcactcg cctcgcgcgg cggcgctctc ggcgagctgc
     6241 gcgtcaccga catcatggtg cgcgaccccg tgaccgcggc ccccgagatg gaagtcaacg
     6301 atctgcggcg caccatgctg gagagcggcg cgcgctacct gccggtgatg cagcagggca
     6361 agcttctcgg cgtgatctcg ttccgcgacg tcgccaaggc ggtgctcgag gagcaggact
     6421 tcgagaacaa gatgctcaag ggttacatca aaaactggcc tgcgtaggcg ttttctccta
     6481 ggtactgcag ccctcgccgc ggccggcact gccgcttggc gttactggcc ggagcaaggg
     6541 ctgtggaatc cctgtctcgc gcagctgccg cggcgccttg ccgagcacga gctggtgcgg
     6601 ggcgcctggg aaggcatcga cgccgcgcag gcctgggaca gccacgcgca cctcgtcggc
     6661 agcggcgatt cggacagcgg catctttgtc aatccgcgca tggagagtct cctcaatccg
     6721 ggcgaatacg cgcggcggct gttcttcctc aacgccggat gcgcgcacag cgcgccggga
     6781 aatgtcgatc gcatctatgt cgagcgcatg cgcaacctgg tcgacggcat gcaaggggaa
     6841 ggaaagcgcg gcgtgaagct gctgctctat gccttcgacc gggtgcacga cgagcgcggc
     6901 gcgcccgaca tggaacgcag cgccttttac gttcccgaca gctacgcgcg ggacactgcg
     6961 aaacgccatc cgggatattt cgaatgggct gcgtcgattc atccatatcg cggcgatgcc
     7021 gcggatgcgc tgcgtaaagc gaagaccgaa ggtgcgcgcg cagtgaagtg gctgccggcc
     7081 gcgatgggga tcgatcccgc gtcgcctcgc tgtgatgcgt tttatgaggt catggcgaag
     7141 ctcgacctgc ccctgatctc tcacgccggc gaagagcgcg cggtgctcgg gcatgacagc
     7201 cacgattacg gcaatccgct caggctgcgg cgcgcgctcg atgccggcgt gcgcgtggtg
     7261 atcgcgcatt gcgcctcgct cggaaaagac cgcgacctcg accagggagc cgacgggcct
     7321 tacgtcgaca gctttgcgct gttcacaagg gtttttgaaa aatatgaaaa gcactgctac
     7381 ggcgacatct cggccatgac gcaggtcaac cgcgccggcc cggcgctcgc gcgcgtcatc
     7441 gaagaaactt cgtggcacgc gcggctgctg aacggctcgg actatccgct gccaggggtg
     7501 atgccgattt tttcggtgga ctacctgatt tcgctcggca tgctggcgcc gaaagccgcg
     7561 cctgtcctga cagagatccg gttgcacaat ccgctgctgt tcgattttgt ggtcaagcgc
     7621 catctgcgct cgaatggcaa ggcgctgtcg gcgagcgtgt tcgagaccag gatgtttttc
     7681 atgagatgat ttccttatgt cgggcaacag cttcggcgtg ctgtactgcg tcacttcatt
     7741 cggcgagtcg cacgggccgg cgtatggcgg cgtggtcgac ggctgcccgc cggggctgga
     7801 gctctcggcc gcggatattc agagagaact ggatcgaaga aagcccggca gttcgcgcca
     7861 tgtgacgcag cgccgcgagt ccgacgcggt ggaaatcctt tccggcgtct accaaggccg
     7921 caccacaggc accgcgatcg gcttcctggt gcgcaacgaa gaccagcgcc cgaaggacta
     7981 ctcggcgatc gccgacaagt tccggcccgg gcacgccgac tacacctact ggcagaagta
     8041 cggcgtccgc ga
//
LOCUS       DASPXW010000324         2354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1232759, whole genome shotgun sequence.
ACCESSION   DASPXW010000324 DASPXW010000000
VERSION     DASPXW010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232759"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..573)
                     /locus_tag="VEL04_07380"
     CDS             complement(175..573)
                     /locus_tag="VEL04_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48226.1"
                     /translation="MNKLSSVFARLLVPAFIVAGIIAAPAMAQEKKASKSAGNERVQK
                     ALIDNGKVRVTETTFKPGQVSPSIERPYRITRVLSGGETLRTYADGKTEKRVFKTGEV
                     FEAGPDKPYSTKNVGKTNIVLYTVTPKQAK"
     gene            665..1501
                     /locus_tag="VEL04_07385"
     CDS             665..1501
                     /locus_tag="VEL04_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HYT48227.1"
                     /translation="MNRRRLLLSLAPALVAPAWLARVGAATMPRASAVPGGVARIGLG
                     ASERAPRARLGGNRVLVMREGDAWVALVGIALAAKPGSRLRVEAEHAGGRYQRFVIDV
                     IPKAYGSQSLKVPPDQVDLSTQDLARYERERAHLDDVLRTFTDSPPATLGMLQPAAGQ
                     RLSTFGLRRYFNDQARNPHAGMDIAAPAGTPVIAANAGRVIDSGDYFFLGRTVVLDHG
                     QGLLSLYAHLEASDASLAQAVSAGTPIGKVGATGRATGPHLHFSVYLNAVAVDPAIFL
                     QP"
     gene            complement(1708..>2354)
                     /locus_tag="VEL04_07390"
     CDS             complement(1708..>2354)
                     /locus_tag="VEL04_07390"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYT48228.1"
                     /translation="RDWPEFNFIIYHACFGPRFFDAEALAAIRSGKLRDGVPDLRWLT
                     EFAQISAPLKNVYAEIGTSFASCVVTFPTVCAHMLGQLMKYMGPERIVFGSDSLWYGA
                     PQWQIEALWRFQIPQDMAKKYGYPQLTADAKRRIFGLNSARLYKLPASVAAYKAVPKD
                     YEARIPDRLKATLNFPGHSRPGSDRGDKLSKLREDYLAAGGHPGNRRYGWIQRS"
BASE COUNT          409 a          749 c          783 g          413 t
ORIGIN      
        1 gagaactgct cgataccatc gcttacccgc cccgcacaaa acggtggtgg aagttctggt
       61 gatctgtggt gggtaggtca acagccggaa gcaggccctg gtatgacccc ctatgcggag
      121 gaatcacaaa caaaaacccc gccgaagcgg ggctgcaggc aatgcgaagc gcggctactt
      181 cgcctgtttc ggcgtgacgg tgtagaggac gatgttggtt ttgccgacat tcttcgtgga
      241 atagggcttg tccggcccgg cctcaaaaac ctctccggtc ttgaaaaccc ttttttcggt
      301 cttgccgtcg gcataggtgc gcagcgtttc gccccccgag agcacgcgcg tgatgcggta
      361 aggccgctcg atgctggggg agacttgtcc tggcttgaag gtggtctccg tcacccggac
      421 cttgccgttg tcgatcaggg ctttctgcac ccgctcgttt ccggcactct tgctcgcctt
      481 tttttcctgc gccatcgcgg gtgctgcgat gatccctgca acaatgaagg ccgggacaag
      541 cagtcttgca aatacgctgg aaagcttgtt catgattcga tcccctccgg gcccgcccgt
      601 ggatgggcag gtcgggtgac tatacgctgg gcgtctgctc tccgcggcta ctagaatccc
      661 ggtgatgaac cggcgtcgct tgctcctgtc gctcgcgccc gcgctcgttg cgccggcatg
      721 gctggcccgt gtgggggccg cgacgatgcc tcgtgcgtcg gcggtgccgg gcggcgtcgc
      781 gagaatcggc ctcggcgcct ccgagcgagc gccgcgagcg cgcctcggcg gcaaccgcgt
      841 gctggtgatg cgcgaaggcg acgcgtgggt cgcgctcgtc ggcatcgcgc tggccgcgaa
      901 gccgggctcg aggctgcggg tggaggcgga gcatgccggc ggcaggtacc agcggttcgt
      961 gatcgatgtc atacccaagg cgtacggctc gcagagcctc aaggtgccgc cggaccaggt
     1021 cgatctctcg acgcaggatc tcgcgcgcta cgagcgcgag cgggcgcatc tggacgacgt
     1081 gctgcgcacg ttcaccgact ccccgccggc gacgctcggc atgctccagc ccgcagccgg
     1141 gcagcgcttg agcaccttcg gcttgcgccg ctacttcaac gaccaggcgc gcaaccccca
     1201 cgccggaatg gacatcgcgg cgcccgcggg cacgccggtg atcgctgcga atgcgggacg
     1261 cgtcatcgac agcggcgact acttcttcct gggccgcacc gtcgtcctcg accacgggca
     1321 gggcctgctc tcgctctacg cccacctgga agcgagcgac gcgagcctcg cgcaagccgt
     1381 gtccgccggc accccgatcg gcaaggtggg cgccaccggc cgtgccactg gaccgcatct
     1441 gcacttctcg gtatatctca acgcggtcgc cgtcgatccg gcgatctttc tgcagcctta
     1501 gaaaaagagg ctttcatgag caaatcggca aagaagaaac gtcctccccg caaaggagat
     1561 gcgcccgcat cgaaacaccg cgaaagtcag gacaaggcga gggatgagaa acaattggag
     1621 tcggaagaca tcgagagagc ggtctatgac ggcatgcagg atttaagggt agaaaggtcc
     1681 aagtaacggt atcgggtcag caaacgatca cgaccgctgg atccacccgt agcggcggtt
     1741 gcccggatgg cctcctgccg caaggtaatc ttcgcgcagc ttcgagagct tgtcgccacg
     1801 gtcgctgccg ggccgcgagt gcccgggaaa gttcaacgtg gccttgaggc ggtcgggaat
     1861 gcgcgcctcg taatccttcg gcacggcctt gtacgccgcc acgctggccg gcagcttgta
     1921 gagtctcgcg gagttcagcc cgaaaatgcg ccgcttggca tcggcggtga gctgcgggta
     1981 gccgtatttc ttcgccatgt cctgcgggat ctggaagcgc cacagggcct cgatctgcca
     2041 ttgcggcgcg ccgtaccaca gcgagtccga gccgaacacg atgcgctccg gccccatgta
     2101 cttcatgagc tgcccgagca tgtgcgcgca aacggtgggg aacgtcacca cgcacgaggc
     2161 gaacgaggtg ccgatctcgg cgtagacgtt cttgagcggc gccgagatct gcgcgaactc
     2221 ggtgagccag cgcaggtccg gcacgccgtc gcgcagcttg ccggagcgga tcgcggcgag
     2281 cgcctcggcg tcgaagaagc gcgggccgaa gcaggcgtgg tagatgatga agttgaactc
     2341 cggccagtcg cgcg
//
LOCUS       DASPXW010000325         2438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_725621, whole genome shotgun sequence.
ACCESSION   DASPXW010000325 DASPXW010000000
VERSION     DASPXW010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725621"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..306)
                     /locus_tag="VEL04_07395"
                     /pseudo
     CDS             complement(1..306)
                     /locus_tag="VEL04_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004522109.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
     gene            complement(392..808)
                     /gene="hemJ"
                     /locus_tag="VEL04_07400"
     CDS             complement(392..808)
                     /gene="hemJ"
                     /locus_tag="VEL04_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HYT48229.1"
                     /translation="MLWVKSLHIISMVTWFAGLFYLPRLFVYHAQADDRASLERFKLM
                     ERRLYRGIMTPGAVLTIVFGVWLWLGWFRGSSSWLHAKIALVVLLVGYHVWCGRLLRD
                     FAAERNTKSQVWLRWFNEFPTLVLVATVFLVVFKPF"
     gene            complement(811..1563)
                     /locus_tag="VEL04_07405"
     CDS             complement(811..1563)
                     /locus_tag="VEL04_07405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYT48230.1"
                     /translation="MMRWVAVWRRNFLVWRKLFIASVLSNLADPLIMLFGLGYGLGAF
                     LPSIEGMSYIAFFAAGSLCSATMFTASFESMFSGFSRMHGQKTWDAILHAPLTIDDVV
                     AGETMWAASKAWLTGSTILAVVVVFGFAASPLVLLALPAAFLVGLAFSAVGLVMTVLA
                     RGWDFFSFYMTLVMTPMMMISGVFFPADRLPAPLVAVARALPLYHGVELVRPVVAGRL
                     PEAAALHVAVLIGYAAAGYAMAIFFARKRFSD"
     gene            complement(1563..>2438)
                     /locus_tag="VEL04_07410"
     CDS             complement(1563..>2438)
                     /locus_tag="VEL04_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYT48231.1"
                     /translation="SYGELEVVGGVDLGVAPGECFGLLGPNGAGKTTTLRLCLGLTRP
                     NAGQIELLGEPVPRRAREARMRVGVVPQFDNLDPDFTVTENLVVYGCYFGIAGRAMRE
                     RVPPLLEFAGLSGRSDAKISTLSGGMKRRLILARALVNDPQLIFMDEPTTGLDPQARH
                     LIWERLRRLTQEGKTLVLTTHAMEEAERLCHRLAIMDHGRIIAQGSPRALIAEHIESH
                     VVEVHGPGYEAWMERAKQLAPRVERAGDTVFCYAQDVEPVMQSLANQSDLVYLHRPAS
                     LEDVFLKLTGRDLRD"
BASE COUNT          493 a          823 c          792 g          330 t
ORIGIN      
        1 ccgctcgacg tcggggcgcc ggccggtcgc gtcgcgaaag cgatcacaca ccgcatcctt
       61 gatccgcaac gtggcgaatt cgaggctctt gagcgggctc ttctgggcgg taacgttcac
      121 cctcagggta cgctcgacgg caaagagccg cggccactcg accgcacgcg ccgcgtcgta
      181 gacgccctgc tcgtcgcgat agctgaaatc ggcgactttc cacagcacgc gcgaagcgat
      241 gcgcgaccga aggttggctg cgtagcagac ttcccagccg ccggagaaag acaccccgcc
      301 ggggacagcg gaagcttgcg aagctttcag agagacgagt tcgcgaagca gcaaatcctc
      361 gagcccgcgc ggcgcgctgg cgaagaacga ctcagaacgg cttgaagaca acgagaaata
      421 ccgttgcgac cagcaccagg gtcgggaatt cgttgaacca ccgcagccag acctggctct
      481 tcgtgttgcg ctcggcggca aaatcgcgca gcaagcgccc gcaccagacg tggtagccga
      541 ccaggagcac gaccagtgcg attttcgcgt gcagccagct ggaagacccg cggaaccagc
      601 cgagccacag ccagacgcca aacacgatgg tcagcaccgc gccgggcgtc atgatgccgc
      661 ggtagagcct gcgctccatc agcttgaagc gctcgagcga ggcgcggtca tcggcctgcg
      721 cgtgatacac gaacaggcgc ggcaggtaaa agagacccgc gaaccaggtc accatggaga
      781 tgatgtgcaa cgacttgacc cagagcatcg ctagtcggag aagcgcttgc gcgcgaaaaa
      841 gatcgccatg gcgtaaccgg cggcagcata gccgatcagc acggcgacgt gcagcgccgc
      901 cgcctccggc aggcggcccg cgaccacggg tcgcaccagc tccacgccgt ggtagagcgg
      961 cagcgcccgg gccaccgcca cgagcggcgc gggcagccgg tcggcaggaa agaatacgcc
     1021 cgagatcatc atcatcggcg tcatgacgag cgtcatgtag aagctgaaga aatcccagcc
     1081 gcgcgcaagc accgtcatca ccagccccac cgcggagaaa gcgagcccga cgagaaatgc
     1141 cgccggcagc gcgagcagaa ccagcggcga agccgcgaaa ccgaacacca ccaccaccgc
     1201 gagaatggtc gacccggtga gccaggcttt gctcgccgcc cacatcgtct cgccggccac
     1261 cacgtcgtcg atggtgagcg gcgcgtgcag gatcgcgtcc caggtcttct gcccgtgcat
     1321 gcgcgagaag ccggagaaca tcgattcgaa ggaggcggtg aacatggtcg cggagcacag
     1381 cgacccggcg gcgaagaagg cgatgtacga catgccttcg atggaaggca ggaatgcgcc
     1441 cagcccgtag cccaggccga acagcatgat caggggatcg gcgaggttgg agagcaccga
     1501 ggcgatgaac agcttgcgcc acaccagaaa attgcgccgc cacactgcga cccagcgcat
     1561 catcaatccc tgagatcccg gccggtgagc ttcaggaata catcctcgag gctcgcgggc
     1621 cggtgcagat aaaccagatc gctctggttg gcgaggctct gcatcaccgg ctcgacgtct
     1681 tgcgcgtagc agaacacggt gtcgccggcg cgctcgacgc gcggcgcgag ctgcttcgca
     1741 cgctccatcc aggcttcata gcccggcccg tgcacctcga ccacgtgcga ctcgatgtgc
     1801 tcggcgatca gcgcgcgtgg cgagccctgg gcgatgatac ggccgtgatc catgatcgca
     1861 agccggtggc acagccgctc ggcctcctcc atcgcgtgcg tggtgaggac gagcgtcttg
     1921 ccttcctgcg tcaggcgccg caaacgctcc cagatcagat gccgcgcctg cggatcgagc
     1981 ccggtcgtcg gctcgtccat gaagatgagc tgcggatcgt tgaccagcgc gcgcgcgagg
     2041 atcagccgcc gtttcatgcc accggagagc gtgctgatct tggcgtcgct gcggccgctc
     2101 aggccggcga attcgaggag cggcggcacg cgctcgcgca tggcgcgccc cgcaatgccg
     2161 aaatagcagc cgtagaccac cagattctcg gtcacggtga agtccgggtc gaggttatca
     2221 aactgcggca cgacgccgac gcgcatgcgc gcctcgcgcg cgcggcgcgg caccggctcg
     2281 ccgagcagct cgatctgccc tgcattcggt cgggtcaggc cgagacatag cctcaacgtc
     2341 gtcgtcttgc ccgcgccgtt gggtccgagc agcccgaagc attcgcccgg cgcgacgccg
     2401 aggtcgacac cgcccaccac ctcgagctcg ccgtagga
//
LOCUS       DASPXW010000326         2878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1482570, whole genome shotgun sequence.
ACCESSION   DASPXW010000326 DASPXW010000000
VERSION     DASPXW010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1482570"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..525)
                     /locus_tag="VEL04_07415"
     CDS             complement(169..525)
                     /locus_tag="VEL04_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HYT48232.1"
                     /translation="MRTLKHAASAALVALILLCLAWELWLAPLRPGGSLIALKALPLA
                     FPLPGIMNGKAYTYKWSSLLILAYLAEGATRAWSDTGLSRNMAFLEIALSVLFFAAAV
                     SYARATQARGNPEETL"
     gene            complement(522..1115)
                     /gene="wrbA"
                     /locus_tag="VEL04_07420"
     CDS             complement(522..1115)
                     /gene="wrbA"
                     /locus_tag="VEL04_07420"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HYT48233.1"
                     /translation="MTEILVLYYSVGGSVRRMAELIAQGVERVPGAASRLRTVPKVSP
                     LTEAASPPVPDSGAPYAELRDLEECAGLALGSPTRFGNMAAPLKHFLDGTALLWQSGA
                     LAGKPACVFTSTSSLHGGQETTLVSMMLPLLHHGMLVMGLPYTETALHATRSGGTPYG
                     ASHFAGLADDQPLTEAERTLCIAQGRRLAEIALKLGK"
     gene            1169..2320
                     /locus_tag="VEL04_07425"
     CDS             1169..2320
                     /locus_tag="VEL04_07425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HYT48234.1"
                     /translation="MLRDALRGSWEFCVAMVQRFREERAMQTAGSLTFTTLLSLVPLL
                     AVVLAASTAFPVFDQAIESLQLFILENLLADAPGIDTVTEQVNSFMRNAGRLTAIGIA
                     GFVVISVMLMLTIDNAMNRIFRVQRRRSTLQIIFVYWAVLSLGPVLIGGSLSLSSFAL
                     GRLQLDAPAGSVLRALPLAFTCAALTMLYGIVPARRVELHHALAGGILAGIGFELAKR
                     GFAIYLAQVPDYTLIYGAFATVPIFLVWLYLSWLVVLAGAIFTAMLPAYYAKPERHRV
                     PGEQLAEALGVLAVLARAHGEGRVMPVNRIARQMRLQPYRCEEVLERAAALGWAARTD
                     REGWVLAREADAIRVQDVYREFVFDSGAVGIGEAELGLSLREYSAREKA"
     gene            2317..>2878
                     /gene="pyrF"
                     /locus_tag="VEL04_07430"
     CDS             2317..>2878
                     /gene="pyrF"
                     /locus_tag="VEL04_07430"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HYT48235.1"
                     /translation="MMPIPPHERLIVALDLPSARDARALVEKLGDAARFYKIGLELFM
                     AGGCFELLDWLVKRGDKVFVDLKFFDVPETVRRAVAALRGRGVSFASVHGNQAMLQAA
                     VKEKGEVKLLAVTVLTSLDRGDLDDLGFSCDVERLVLSRARRAMQAGCDGVISSGLEA
                     APLKAEFKDRLLVVAPGIRPVDNRPAD"
BASE COUNT          423 a          969 c         1007 g          479 t
ORIGIN      
        1 tggtgaggcg cacggcgtcc ggtttccaca aggtaaaggc gttgaccggc cccgcggacc
       61 aggagcggca ggaagcacag tggcagtagc ccatggcggc cggttcgccg ctgacccgga
      121 tctccacggc gccgcagaag cagcttcccc ggtaactctt gtcattgctc atagcgtctc
      181 ctcaggattg ccgcgagctt gcgttgcacg cgcgtagctt acagccgcgg cgaaaaaaag
      241 cacgctgagc gcgatttcca ggaaagccat gttccgggaa agcccggtgt cgctccaggc
      301 gcgcgtcgcg ccttcggcga ggtaggcgag gatcagcagg ctcgaccact tatacgtata
      361 ggctttccca ttcatgatcc cggggagggg aaaggccagc ggcagggcct tgagcgcgat
      421 cagcgaccct cccggccgca gcggcgcgag ccacagttcc catgcgaggc agagcaggat
      481 cagggcgacc agcgccgctg acgctgcatg cttcaaagtc ctcattttcc cagcttgagc
      541 gcgatctccg ccagccgcct gccctgcgcg atgcacagcg tgcgctcggc ctcggtgagc
      601 ggctggtcgt cggcgaggcc ggcgaagtgg ctcgcgccgt agggggtgcc gccgctgcgc
      661 gtcgcgtgca gcgcggtctc ggtgtagggc aggcccatca ccagcatgcc gtgatgcagc
      721 agcggcagca tcatcgaaac gagcgtggtc tcctgcccgc cgtgcaggct cgaggtcgag
      781 gtgaacacgc aggcgggctt gccggcgagc gcgccgcttt gccacagcag cgcggtgccg
      841 tcgagaaaat gcttgagcgg ggccgccatg ttgccgaagc gcgtggggct gccgagcgcg
      901 agcccggcgc attcctcgag gtctcgcagc tcggcatagg gagcgccgga atccggcacc
      961 gggggagatg cggcttccgt caaaggcgaa accttcggca ccgtgcgcag ccgcgacgcg
     1021 gcgccgggca cgcgctcgac gccctgcgcg atcagctcgg ccatgcggcg caccgacccg
     1081 cccacgctgt agtacaagac gagtatctcc gtcatcgtga tctgtacagg acgagaaatt
     1141 cgaccatgac ggtatgataa aaggattgat gctccgggac gcgctgcgcg gttcgtggga
     1201 attctgcgtc gccatggtgc agcgcttccg cgaagagcgc gccatgcaga ctgcgggcag
     1261 cctcaccttc accacgctgc tttcgctggt gccgctgctc gccgtggtgc tcgcggcctc
     1321 gaccgcgttc ccggtgttcg accaggcgat cgagtcgctg cagctcttca tcctggaaaa
     1381 cctgctggcg gacgcccccg gcatcgacac cgtcaccgag caggtcaatt ccttcatgcg
     1441 caacgccggc cggctcaccg ccatcggcat tgccggcttc gtcgtgatct cggtgatgct
     1501 gatgctgacc atcgacaacg ccatgaaccg cattttccgc gtgcagcggc gccgctcgac
     1561 gctgcagatc atcttcgtgt actgggcggt gctgtcgctc gggccggtgc tgatcggcgg
     1621 cagcctgtcg ctcagctcgt tcgcgctcgg ccggctgcag ctcgacgctc cggccggctc
     1681 ggtgctgcgc gccctgccgc tcgctttcac ctgcgccgcc ctgaccatgc tctacggcat
     1741 cgtgccggcg cgccgcgtcg agctgcacca tgcgctcgcc ggcggcatcc tcgccggcat
     1801 cggcttcgag ctggcgaagc gcggcttcgc gatctatctc gcgcaggtcc cggactacac
     1861 gctgatctac ggcgccttcg ccaccgtgcc gatcttcctg gtgtggctgt acctgtcctg
     1921 gctggtggtg ctggccggcg cgatcttcac ggcgatgctc ccggcctact acgccaagcc
     1981 cgagcggcac cgcgtgccgg gcgagcagct cgccgaggcg ctcggcgtgc tcgcggtgct
     2041 ggcgcgcgca cacggcgaag gacgcgtgat gccggtcaac cgcatcgcca ggcagatgcg
     2101 gctgcagccc taccgttgcg aggaggtgct cgagcgcgcc gccgcgctcg gctgggcggc
     2161 gcgcaccgac cgggagggct gggtgctcgc gcgcgaagcc gacgccatcc gcgtccagga
     2221 cgtgtaccgc gagttcgtgt tcgacagcgg cgcggtcggc atcggcgagg ccgagctcgg
     2281 gctgtcgctg agggaatatt ccgccaggga gaaggcatga tgccgattcc gccgcacgag
     2341 cggctgatcg tcgcgctcga cctgccgagc gctcgcgatg cgcgcgcgct ggtcgagaag
     2401 ctcggcgacg ccgcgcgttt ctacaagatc ggcctggagt tgttcatggc gggcggctgc
     2461 ttcgagctgc tcgactggct ggtgaagcgc ggcgacaagg tgttcgtcga cctcaagttc
     2521 ttcgacgtgc ccgagactgt gcggcgcgcg gtggccgccc tgcgcggccg cggcgtgagc
     2581 ttcgccagcg tgcacggcaa ccaggcgatg ctgcaggcgg cggtgaagga gaagggcgag
     2641 gtgaagctgc tcgcggtcac ggtgctcacc agcctcgatc gcggcgatct cgacgatctc
     2701 gggttttcct gcgacgtcga gcgcctggtg ctgtcgcgcg cgcgccgcgc gatgcaggcg
     2761 ggctgcgacg gcgtgatctc ctccggcctc gaggcggcgc cgctgaaggc cgaattcaag
     2821 gaccggctgc tggtggtggc gccggggatc cggccggtcg ataaccggcc cgccgatg
//
LOCUS       DASPXW010000327         5951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1482706, whole genome shotgun sequence.
ACCESSION   DASPXW010000327 DASPXW010000000
VERSION     DASPXW010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5951
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1482706"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="VEL04_07435"
     CDS             <1..806
                     /locus_tag="VEL04_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48236.1"
                     /translation="MNELLSNPAVQAGAAPFVAALVVALALQPLRLGGLAVTAAFLVA
                     MQLVSGIQFSPLTATRKLVLLAIAAPLVGVVADFAFKPTRLGAMVLALLAAAGALWAF
                     EPVLVQRPAAEAWLLGAAAAIALAFMVGFAQTFLAADAVRAGAAALALGLGAGVAAIF
                     SASAAYGSYGIALGAGAGAFLLPQMITGRRSFAGATFTLPAMLTGGLVAVGAMLLAKL
                     PWYALLVLAAVPLAVRLPGPERAPVWLQAVIFSLYGFAVAGFACFLAWP"
     gene            833..1426
                     /locus_tag="VEL04_07440"
     CDS             833..1426
                     /locus_tag="VEL04_07440"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HYT48237.1"
                     /translation="MRRVVIAAALAAPFAASAQVENYVIDPFHTVPYFEADHLGFATM
                     RGRFDRATGKFSIDRAAKTGAVEIVIPTDKVSTGDTEREGRARTRDEHLRTVDFFNVQ
                     EFPSMTFRSTRVIFKGDDPETVEGNLTLLGVTKPVALKLERWKCGPDMRTQGKRYQCG
                     GNATGSFKRSEFGMKYGLPAAIGDEVKLWMSFYGFRQ"
     gene            complement(1416..3527)
                     /gene="ppk1"
                     /locus_tag="VEL04_07445"
     CDS             complement(1416..3527)
                     /gene="ppk1"
                     /locus_tag="VEL04_07445"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003263620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HYT48238.1"
                     /translation="MNRRISIPTQFLNRELGILAFNRRVLAQAADDSVPPLERLRFIT
                     VVSSNLDEFFEIRVAGLKESIKLGLAEPGPDGRSAADVFAQVSREARALVAEQYQLLN
                     QTVLPALAQEGVVFPKREEWNAAQRQWIRDYFFRELLPVLTPIGLDPAHPFPRVFNKS
                     LNLAVELEGRDAFGRSSGAAIVQAPRALPRVIRLPAEIAGAEYAFVFLSSILHAHVGE
                     LFAGMNVQGCYQFRVTRNSDLFVDEEEIKNLHTALQGELFHRHFGDAVRLEVADNCSQ
                     QMVEFLLQQFGLQETDLYRVEGPVNLYRLREVPDQVDRLQLKYPPFQPGLPPALDSKD
                     LFEVLKKQDVLLHHPFQSFLPVIDFIRTTANDPNVIAIKQTVYRTGTDSELMDILVDA
                     ARKGKEVTVVVELMARFDEEANLNWAARLEEVGAHVIYGVVGHKTHAKMSLIVRREET
                     GQGKVLRRYVHLATGNYHPSTARQYTDFGLLTANEEMCADVAEVFKQLTGLGRAGKMK
                     HLWQSPFTLHKRIVAAIRNEAAQAAEGKPARIIAKMNALLEPQIIEELYAASAAGVKI
                     DLIVRGVCALKPGITGLSENIRVRSLVGRFLEHSRVFYFANGGADDVYLASADWMDRN
                     FFRRIELCFPVLDPALKRRVIGEGLQPYLDDNCQAWLMNAEGEYERLKPRRGRSRSAQ
                     EELLFTLAIPIAVRQETKATA"
     gene            3720..5240
                     /gene="ppx"
                     /locus_tag="VEL04_07450"
     CDS             3720..5240
                     /gene="ppx"
                     /locus_tag="VEL04_07450"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HYT48239.1"
                     /translation="MARHETLAAVDLGSNSFHLQIGRVVDGQIYPLDAVREVIRLGAG
                     LTAEKRIDRATQAAALEALAKFAERLRGFPRQAVRAVGTNALRVAKNSPQFLREARAV
                     LGFPIEVISGREEARLIYLGVAHALPAAAHRRLVLDIGGGSTELIIGTGFEPQLTESL
                     YMGCVSFSLKYFPDGKIDKPRMKAAELAARQELAGMVGTYRAAGWDEAVGSSGTARAL
                     ENILRENGFAQEGLTREGLERLRSLLVKHEEADAERIAGLRPNRAPVLPGGLAIMSAA
                     LDELGIEAMKVSDGALRHGVLYDLLGRVQHRDMREATVAQFMRRYHVDAAQAERVKGL
                     SLTIYDALTPGAEREDDPDRLMLEWTARLAEIGLSIAHAQYHKHSAYVLSNADMPGFS
                     RMEQARLSRVVLAHRGKLSKVQDAGLEGPDWKLVFALRTASLILRSRSDAKLPFLRVA
                     ADDAGFAIDLPQSWLDDNALSAAALDAEAGHWKAVGMKLEVSELSDKKVSVLKQIR"
     gene            complement(5223..5657)
                     /locus_tag="VEL04_07455"
     CDS             complement(5223..5657)
                     /locus_tag="VEL04_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008342020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HYT48240.1"
                     /translation="MELILWRHADAEDGAPDLERKLTKKGRKQSARVAEWLLARLPKE
                     FVVIASPAARAQETAGALGVRVKTADQLAPGASVAKILKAAGWPDAEQTVVVVGHQPD
                     LGRAAAHLVCGAQAEWSIKKGGLWWLADEPAEVRAVISPDLL"
     gene            5685..5761
                     /locus_tag="VEL04_07460"
     tRNA            5685..5761
                     /locus_tag="VEL04_07460"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5719..5721,aa:Val,seq:gac)
     gene            5885..>5951
                     /locus_tag="VEL04_07465"
     CDS             5885..>5951
                     /locus_tag="VEL04_07465"
                     /inference="COORDINATES: protein motif:HMM:NF025088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48241.1"
                     /translation="MGIDPLELREGELPPRVLGLVM"
BASE COUNT          912 a         1986 c         2032 g         1021 t
ORIGIN      
        1 tgatgaacga gctgctctcc aatcccgcgg tgcaggcggg cgcggcgcct ttcgtcgcag
       61 cgctcgtggt cgcgctcgcg ctgcagccgc tcaggctcgg cgggctcgcc gtgaccgcgg
      121 cattcctggt ggcgatgcag ctcgtgtccg ggatccagtt cagcccgctt accgcgacgc
      181 gcaagctcgt gctcctcgcc attgccgcgc cgctggtcgg cgtagtcgcg gattttgcct
      241 tcaagccgac gcggctcggc gcgatggtgc tggcgctgct cgcggcggca ggggccttat
      301 gggctttcga gccggtgctg gtgcagcgcc cggccgccga ggcctggctg ctcggcgcgg
      361 cagcggccat cgcgctcgcc ttcatggtcg gcttcgcgca gacttttctc gccgccgacg
      421 cggtacgcgc gggcgctgcc gcgctcgcgc tggggctggg cgccggggtg gcggcgattt
      481 tttcggcgtc ggccgcctac ggctcgtacg gtatcgcgct cggcgcgggc gccggggcat
      541 tcctgctgcc gcagatgatc acgggcagga gaagctttgc cggcgcgacc tttacgctgc
      601 cggcaatgct caccggcggc ctggttgccg tgggcgcgat gctgctcgcc aaacttccct
      661 ggtacgcgct gctggtgctc gccgccgtgc cgctggcggt gcgcctgccg gggcccgagc
      721 gggcaccggt ctggcttcag gcggtgatat tctcgctgta cggctttgcc gtcgcaggat
      781 tcgcctgctt cctggcctgg ccttgatcgc ctcaacccta gaggagggga gcatgagaag
      841 agttgtgatt gcggccgcgc tcgcggctcc gttcgctgcg tcggcccagg tcgagaacta
      901 cgtcatcgac ccgttccaca ccgtgccgta tttcgaggcc gaccacctcg gctttgccac
      961 catgcgcggc cgtttcgacc gcgccaccgg aaaattcagc atcgaccgcg cagcgaagac
     1021 cggcgcggtc gagatcgtca ttccgaccga caaggtcagc acgggcgaca ccgagcgcga
     1081 ggggcgcgcg cgcacgcgcg acgagcacct gcgaaccgtg gacttcttca acgtgcagga
     1141 atttcccagc atgaccttca ggtcgaccag ggtgatcttc aagggcgacg atcccgagac
     1201 cgtcgagggc aatctgacgc tgctcggcgt gaccaagccg gtcgccctga agctcgagcg
     1261 ctggaagtgc ggcccggaca tgcgcaccca gggcaagcgc taccagtgcg gcggcaacgc
     1321 caccgggtcc ttcaagcgct cggagttcgg catgaaatac ggcctgccgg cagcaatcgg
     1381 cgacgaggtg aagctctgga tgagcttcta cggcttcagg cagtagcttt cgtttcctgc
     1441 cgtaccgcga tggggatggc gagggtgaac agcagctcct cctgggccga gcggctgcgg
     1501 ccgcggcgcg gcttcagccg ctcgtattcg ccttcggcat tcatcagcca ggcctggcag
     1561 ttatcgtcca ggtacggctg caagccttcg ccgatcacgc ggcgcttgag cgccgggtcg
     1621 agcaccggaa agcacagctc gatgcggcgg aagaagttgc ggtccatcca gtcggcgctc
     1681 gcgagataca cgtcgtcggc gccgccgttc gcgaagtaga acacgcgcga gtgctcgaga
     1741 aagcggccga ccaacgagcg cacccggatg ttttccgata gcccggtgat gccgggcttc
     1801 agcgcgcaca cgccgcgcac gatgagatcg atcttcacgc cggccgccga cgcggcgtag
     1861 agctcctcga tgatctgcgg ctcgagcagc gcgttcatct tggcgatgat gcgcgcgggt
     1921 ttgccttcgg ccgcctgcgc ggcctcgttg cggatcgcgg cgacgatgcg cttgtggagc
     1981 gtgaacggcg actgccacag gtgcttcatc ttgccggcgc ggccgaggcc ggtgagctgc
     2041 ttgaatacct cggcgacatc ggcgcacatt tcctcgttcg ccgtcagcag gccgaagtcg
     2101 gtgtactgcc gcgcggtgct ggggtggtaa ttcccggtcg cgagatggac gtagcggcgc
     2161 agtaccttgc cctgcccggt ttcctcgcgc cgcacgatga gcgacatctt ggcgtgcgtc
     2221 ttgtggccga ccacgccgta gatcacgtgc gcgccgactt cttcgaggcg cgcggcccag
     2281 ttgaggttcg cttcttcgtc gaacctcgcc atcagctcga ccaccacggt cacttccttg
     2341 cccttgcgcg ccgcgtcgac caggatatcc atcagctcgg agtcggtgcc ggtgcggtac
     2401 acggtctgct tgatcgcgat cacgttcggg tcgttcgccg tggtgcggat gaagtcgatg
     2461 accggcagga acgactggaa cggatgatgc agcaagacgt cttgcttctt cagcacctcg
     2521 aacaggtcct tggagtcgag cgccggcggc agtcccggct ggaacggcgg gtacttgagc
     2581 tgcaggcggt cgacctggtc gggcacctcg cgcagccggt agaggttgac cgggccttcg
     2641 acgcggtaca ggtccgtctc ctgcaggccg aactgctgca gcaggaactc gaccatctgc
     2701 tgcgagcagt tgtcggccac ctcgaggcgc acggcgtcgc cgaaatggcg gtggaacagc
     2761 tcgccctgca gcgcagtgtg caggttcttg atctcctcct cgtcgacgaa caggtccgaa
     2821 ttgcgcgtga cgcggaactg gtagcagccc tgcacgttca tgccggcgaa cagctcgccg
     2881 acgtgggcgt gcaggatcga cgacaggaac acgaacgcgt attcggcgcc ggcgatctcc
     2941 gccggcaggc ggatgacgcg cggcagggcg cgcggcgcct gcacgatcgc ggcgcccgag
     3001 ctgcggccga aggcgtcgcg cccctcgagc tcgaccgcga gattgaggct cttgttgaac
     3061 acgcgcggga acgggtgcgc gggatcgagg ccgatcggcg tcagcaccgg cagcagctcg
     3121 cggaagaagt agtcgcggat ccactgccgc tgcgccgcgt tccactcctc gcgcttcggg
     3181 aacaccacgc cctcctgcgc gagcgcaggc agcacggtct ggttcagcag ctggtactgc
     3241 tcggcgacca gcgcgcgcgc ctcgcggctc acctgcgcga acacgtcggc agcgctgcgg
     3301 ccgtccgggc cgggctcggc gaggccgagc ttgatgctct ccttcaggcc ggcgacgcgg
     3361 atctcgaaga actcgtccag gttcgaggac acgacggtga tgaagcgcag ccgctcgagc
     3421 ggcggcaccg agtcgtcggc cgcctgcgcc agcacgcggc ggttgaacgc caggatgccg
     3481 agctcgcggt tgaggaactg cgtcgggatg gagattctgc ggttcatcgc ctgcgcggga
     3541 gcgggaagcg gaggagccat tggactgacc gctgcggccg gagaaatgac gcgctccggc
     3601 ccgagttcct gctgcgaccc ctcggactgc atttagacaa cctcgttttc ccaagtttgc
     3661 acgcaaagcg actctccatg cgtgacagat tcgtttcacg ccgttaactt cttgaattca
     3721 tggcacgtca cgaaacgctt gccgcagtag acctcggctc gaacagcttc cacctgcaga
     3781 tcggcagggt cgtggacggc cagatctatc cgctcgatgc ggtgcgcgag gtaatccggc
     3841 tcggcgccgg gctcacggcc gagaagcgca tcgaccgcgc gacgcaggcc gcggcgctcg
     3901 aggcgctcgc gaagttcgcc gagcggctga ggggctttcc gcgccaggcg gtgcgcgccg
     3961 tcggcaccaa tgcgctgcgc gtggccaaga actcgccgca gttcctgcgc gaggcgcgcg
     4021 ccgtgctcgg cttcccgatc gaggtgatct cgggccgcga ggaggcgcgc ctcatctacc
     4081 tgggcgtggc gcacgcgctg cccgcggcgg cacaccggcg cctggtgctc gacatcggcg
     4141 gcggctcgac cgagctcatc atcggcaccg gattcgagcc gcagctcacc gagtcgctct
     4201 acatgggctg cgtgagcttc agcctgaagt attttcccga cggcaagatc gacaagccgc
     4261 gcatgaaagc cgccgagctc gccgcgcgcc aggagctcgc cggcatggtc gggacctacc
     4321 gcgcggcggg ctgggacgag gcggtcggct catcgggcac cgcgcgcgcg ctggagaaca
     4381 tcctgcgcga gaacggcttc gcccaggagg gcctcacgcg cgaaggcctc gagcggctga
     4441 gaagcctgct cgtcaagcac gaggaggcgg acgccgagcg catcgccggg ctgcggccca
     4501 accgcgcccc ggttctgccc ggcggcctgg cgatcatgag cgcggcgctc gacgagctcg
     4561 gcatcgaggc gatgaaggtc tccgacggcg cgctgcgtca cggcgtgctg tacgacctgc
     4621 tcggccgcgt gcagcaccgc gacatgcgcg aggcgacggt ggcgcagttc atgcgccgct
     4681 accacgtcga cgccgcgcaa gccgaaaggg taaagggatt gtcgctgacg atttacgacg
     4741 cgctcacgcc gggcgccgag cgcgaggacg atcctgaccg cctgatgctc gaatggacgg
     4801 cgcggctcgc cgagatcggc ctctcgatcg cgcatgccca gtaccacaag cattccgcgt
     4861 acgtgctctc gaatgccgac atgccgggct tctcgcggat ggagcaggcg cgcctttcgc
     4921 gcgtcgtgct cgcgcaccgc ggcaagctct ccaaggtgca ggacgccggc ctcgaggggc
     4981 cggactggaa gctggtgttc gcgctgcgca ccgcttcgct catcctgcgc agccgcagcg
     5041 atgcgaagct tccgttcctg cgcgtggcgg ccgacgacgc aggcttcgcg atcgatctgc
     5101 cgcagtcctg gctggacgac aacgcgctgt cagcggcggc gctcgatgcc gaagccggcc
     5161 actggaaagc ggtgggaatg aagctcgagg tgagcgagct ctccgacaag aaggtgtcgg
     5221 ttttaaagca gatcaggtga gatcaccgcc cgcacctccg cgggctcgtc ggcgagccac
     5281 cacaggccac ccttcttgat gctccattcc gcctgcgcgc cgcatacgag gtgagcagcg
     5341 gcgcggccga gatcgggctg gtggcccacg accacgacgg tttgttcggc gtcgggccat
     5401 ccggccgctt tcaggatctt tgccaccgag gctcccggcg cgagctgatc cgcggtcttg
     5461 acgcgcaccc cgagcgctcc cgcggtttct tgcgcacgcg cagccgggct cgcgatcacc
     5521 acgaactcct tgggcaggcg cgcgagcaac cactcggcga cgcgcgcgga ctgcttgcgt
     5581 cccttcttcg tcagcttgcg ctcgaggtcc ggcgcgccgt cctcggcgtc ggcatgccgc
     5641 cagagaatga gctccatgtg cttagtataa aatttgtgcc ttgcaggcgc gtagctcagc
     5701 tggttagagc actaccttga catggtaggg gtcgttggtt cgagtccaat cgcgcctacc
     5761 agctcccctc accaaagcta ttcggcagcg taggataacc cgtgcctgaa atctcgcgct
     5821 ttctgggaat cgtcatcact atgtattgca ctttcatgtg cgctacgaaa acttccgtgc
     5881 gattgtggga atcgatccgc tcgagctgcg cgaaggcgag ctgccgccgc gcgtcctcgg
     5941 ccttgtgatg g
//
LOCUS       DASPXW010000328         2871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1607325, whole genome shotgun sequence.
ACCESSION   DASPXW010000328 DASPXW010000000
VERSION     DASPXW010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1607325"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VEL04_07470"
     CDS             complement(<1..525)
                     /locus_tag="VEL04_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT48242.1"
                     /translation="MILEVSGLTVQFPTRRGTLTAVNGISFSIAPGEVLGVVGESGAG
                     KSITGLAIIGLLETPGRIAAGEVRLEGRRIDHLKHEEMRRIRGRKIGVVFQDPLTSLN
                     PLYTIGGQLEETILAHLPLDRAQARGRALSLLSEVGIPAPEARYEHYPHHFSGGMRQR
                     VVIALALAAEPKLLI"
     gene            complement(522..1430)
                     /locus_tag="VEL04_07475"
     CDS             complement(522..1430)
                     /locus_tag="VEL04_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYT48243.1"
                     /translation="MILRRVWDSDLAWSFRRSPVTVAAAALTLICVGAAVFAPWLAPQ
                     DPFDMAALDLNDAFTPPGWDVKGLARYPLGTDNQGRDILSTIMHGARISLGVGLASVL
                     FALLLGVSLGLISGYFGGRIDAVIMRVADVQLSFPAILIALLIDGVARVALPSERHDE
                     IAIPVLVLAIGFAGWVQYARTVRGSTMVEKGKEYVQAARVIGRHPLAIMLSHVLPNVI
                     GPVLVIATIHIATAIITEATLSFLGVGVPATRPSLGTLIRVGNEFLYSGEWWITLFPG
                     LALVILVLSVNLLGDWLRDALNPKLR"
     gene            complement(1427..>2871)
                     /locus_tag="VEL04_07480"
     CDS             complement(1427..>2871)
                     /locus_tag="VEL04_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYT48244.1"
                     /translation="ATTLSILQYAYEGLVRYTRDYEVEPSLAVSWNELSPTHWRFNLR
                     KGVKFHDGSPFTADDVVFSFGRLRQPQGTMQIYVAGISEVKKVDDYTVDFILSGPHPI
                     LLRNIIDFRIMSKSWSEKNRSQNIQDYKAKEETYASRNTNGTGPYLIKTWEPDKRIVF
                     AQNKDWWGKLDGNVTDVIYTPIKSDATRVSALLSGDVDLVTDLPTQDVERLRKDANIK
                     VLDGHEVRTIFIGMDQYSDELKYSNVKGKNPFKDLKVRRALNTAVDREAIRKVTMRGL
                     SIPAGIMVAPGVHGYSKQLDIVPKYDPAGAKKLLAEAGYPDGFEFTLDCPNNRYVNDE
                     KICQALVAMWARAGFRVKLNAIPFANFIPKILNLDTSAYMLGWGVQTFDALYTLQSLV
                     RTKTTGADGSFNQGRISDPKLDTTIDAIKIATDPKARDALLKEGLEVTRDQAYYVPLH
                     HQLRPWAMKRNVIAVHSPDDRPQARFFTVK"
BASE COUNT          483 a          915 c          969 g          504 t
ORIGIN      
        1 gatcagcagc ttcggctcgg cggcgagcgc gagcgcgatg accacgcgct ggcgcatgcc
       61 gcccgagaag tggtgcggat agtgctcgta gcgcgcttcg ggcgccggaa tgccgacttc
      121 gctcagcagc gaaagggcgc ggccgcgcgc ctgcgccctg tccagcggca ggtgcgcgag
      181 gatcgtttcc tcgagctgcc cgccgatggt gtagagcgga ttcagcgaag tcagcgggtc
      241 ctggaacacg acgccgatct tgcggccgcg gatgcggcgc atttcctcgt gtttcaggtg
      301 gtcgatgcgc cggccctcga ggcgcacctc gccggcggca atgcgcccgg gcgtctcgag
      361 caggccgatg atcgcgagcc cggtgatcga tttgccggcg cccgattcgc cgaccacgcc
      421 gagcacctcg cccggggcga tggaaaacga aatgccgttc accgcggtca aggttcctcg
      481 ccgcgtcggg aattgaacgg tcaaacctga aacctccaga atcatcggag cttcgggttg
      541 agcgcgtcgc gcagccagtc gcccagcagg ttgaccgaca gcaccaggat caccagcgcg
      601 aggcccggga acagcgtgat ccaccattcg ccggaataga ggaactcgtt gccgacacgg
      661 atcagcgtgc cgagcgaggg ccgcgttgcc ggcacgccga cgccgaggaa ggacagcgtc
      721 gcctcggtaa tgatcgcggt cgcgatatgg atggtcgcga tcaccagcac cgggccgatc
      781 acgttcggca gcacatgcga cagcatgatg gcgagcgggt ggcggccgat gacgcgggcg
      841 gcctggacgt actccttgcc cttttccacc atcgtcgagc cccgcacggt gcgcgcgtac
      901 tgcacccagc cggcgaagcc gatcgccagc acgaggaccg ggatcgcgat ctcgtcatgg
      961 cgctcgctgg gcagcgcgac gcgcgccacg ccgtcgatca gcagcgcgat caggatcgcc
     1021 gggaacgaga gctgcacgtc ggcgacgcgc atgatgacgg cgtcgatgcg tccaccgaag
     1081 taaccggaaa tcaatcctag agaaacccct aaaagcaggg cgaacaatac cgaagccaac
     1141 ccgacgccga gcgagatgcg cgcgccgtgc atgatcgtgg acaaaatgtc gcggccctgg
     1201 ttgtcggtgc ccagcggata gcgcgccagc cccttcacgt cccagcccgg cggcgtgaag
     1261 gcgtcgttca ggtcgagcgc cgccatgtcg aacgggtcct gcggcgcgag ccagggcgcg
     1321 aataccgcgg cgccgacgca gatcagcgtc agcgctgctg cggcgacggt gaccggcgac
     1381 ctgcggaagc tccaggcaag atcgctgtcc cagacccgcc gcagaatcac ttcacggtga
     1441 agaagcgcgc ctgcggccgg tcgtcggggc tgtgcaccgc gatgacgttc cgcttcatcg
     1501 cccacggcct cagctggtgg tgcagcggga cgtagtaggc ctggtcccgg gtcacctcga
     1561 gcccctcctt gagcagcgcg tcgcgcgcct tgggatcggt ggcgatcttg atcgcgtcga
     1621 tggtggtatc gagcttcgga tcgctgatgc gcccctggtt gaagctgccg tcggcgccgg
     1681 tggtcttggt gcgcaccagg gactgcagcg tgtagagcgc gtcgaaggtc tgcacccccc
     1741 agcccagcat gtaggcgctc gtatcgaggt tgaggatctt cgggatgaag ttggcgaacg
     1801 gtatcgcgtt gagcttgacg cggaatccgg cgcgggccca catcgcgacc agcgcctggc
     1861 agatcttctc gtcgttcacg tagcggttgt tgggacagtc gagcgtgaac tcgaagccgt
     1921 ccggatagcc cgcctcggcc agcagcttct tcgcgccggc ggggtcgtac ttcggcacga
     1981 tgtcgagctg cttggaatag ccgtgcactc caggggccac catgatgccc gccgggatcg
     2041 acaggccgcg catcgtgacc ttcctgatcg cctcgcgatc caccgccgtg ttcaacgcac
     2101 ggcgcacctt caggtccttg aacgggttct tgcccttcac gttcgaatac ttgagctcgt
     2161 cgctgtactg gtccatgccg atgaagatgg tgcgcacctc gtggccgtcg agcactttga
     2221 tgttggcgtc cttgcgcagc cgctcgacgt cctgcgtcgg caggtcggtg acgaggtcga
     2281 cgtcgcccga gagcagcgcc gagacgcgcg tggcgtccga cttgatcggc gtatagatca
     2341 cgtcggtcac gttgccgtcg agctttcccc accagtcctt gttttgcgcg aacacgatgc
     2401 gcttgtcggg ctcccaggtc ttgatcaggt aagggccggt gccattcgtg ttgcgcgagg
     2461 cgtaggtctc ctccttcgcc ttgtagtcct ggatgttctg cgagcggttc ttctccgacc
     2521 aggacttgct catgatgcgg aagtcgatga tgttgcgcag cagaatggga tgcggtccgg
     2581 agaggatgaa gtcgaccgtg tagtcatcga ctttcttcac ctcggagatg cctgcgacgt
     2641 agatctgcat cgtgccctgc ggttgccgca ggcgcccgaa ggagaacacc acgtcgtcgg
     2701 cagtgaaggg cgagccgtcg tggaacttga cgcccttgcg caggttgaag cgccagtgcg
     2761 tcggcgacag ctcgttccag ctcaccgcca gcgacggctc gacctcgtag tccctggtgt
     2821 agcgcaccag gccttcgtag gcgtactgca ggatcgagag cgtggtggcg t
//
LOCUS       DASPXW010000329         3028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_99155, whole genome shotgun sequence.
ACCESSION   DASPXW010000329 DASPXW010000000
VERSION     DASPXW010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99155"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..1593)
                     /locus_tag="VEL04_07485"
     CDS             complement(43..1593)
                     /locus_tag="VEL04_07485"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HYT48245.1"
                     /translation="MNKDRLIIFDTTMRDGEQSPGASMTRDEKLRIARALERMRVDVI
                     EAGFPASSNGDFEAVRAVASVIRESTVCGLCRANDRDIQRGIDALKGARGPWRIHTFI
                     ATSALHMEKKLRMTPQQVLEAAKLAVRYAKQACPDVEFSPEDGSRSDPDFLCRVLEAA
                     IREGATTINIPDTVGYAVPQQFGEFMKQLREKIPNSDRAVWSVHCHNDLGMAVANSLA
                     AVMIGGARQIECTINGLGERAGNTSLEEVVMAVKTRGDFFNLKTGIDTTHIVPASRLV
                     AQITGFVVQPNKAVVGANAFAHASGIHQDGVLKARQTYEIMRAEDVGWAANKIVLGKL
                     SGRSAFRQRMKELGVELESEDAYNKAFQRFKDLADKKADIFDEDLLALVAQEAGRSAE
                     EQWKLVTMNQASGMGERPHATVVLKEDGLEKRSESQGDGPVDATFKAIEAIAKSGAEL
                     LLYSVNAVTTGTESQGEVTVRLAKGGRIVNGVGADTDIVVASAKAYISALNKLASKME
                     RLNPQHQL"
     gene            complement(1847..2185)
                     /locus_tag="VEL04_07490"
     CDS             complement(1847..2185)
                     /locus_tag="VEL04_07490"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HYT48246.1"
                     /translation="MRRGEIWWASLPEPTGSGPGFRRPVVIVQADSFNASRIRTAIVA
                     VITSNLRLAEAPGNVLLRARSTRLKRDSVANVSQLITLDRSVLTERIGRLPPAQQQQL
                     DEGLRLALEL"
     gene            complement(2186..2425)
                     /locus_tag="VEL04_07495"
     CDS             complement(2186..2425)
                     /locus_tag="VEL04_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48247.1"
                     /translation="MKTAISIPDPIFQSAERAAKRLKLSRSQLYAAAVAAYLERNRDS
                     SVTAKLNEVYADANASLVEAGLAAAQLRSIARERW"
     gene            2593..>3028
                     /locus_tag="VEL04_07500"
     CDS             2593..>3028
                     /locus_tag="VEL04_07500"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HYT48248.1"
                     /translation="MKTLLAAACLALASAAANAGETINIYKYRGADGRPLYSDRPIPG
                     ARLIESIQYVLPAPSSPRSDTSKSGAAGEERIKNHLSELDAAWAEVKASGSALAAAEA
                     RLAAGVTPGEGEPRALAGPPTPAPPSAGGPMATAPAPAGGPLP"
BASE COUNT          500 a         1018 c          965 g          545 t
ORIGIN      
        1 cgggaagcga aaatgcgagc agcacggcaa tggtgagtgt tttcatagtt gatgttgagg
       61 attgagtcgc tccattttgc tggcgagctt gttcagcgcg ctgatgtagg ccttggccga
      121 cgctaccacg atatccgtat cggcccccac gccgttcacg atgcgcccgc ccttcgccag
      181 gcgcaccgtg acctcgccct gcgattcggt gccggtggtc acggcgttca ccgaatatag
      241 cagcagctcg gcgccgcttt tcgcgatggc ctcgatcgcc ttgaaggtgg cgtccaccgg
      301 gccatcaccc tgcgattcgg aacgtttctc gagcccatcc tccttcaaga ccaccgttgc
      361 atgcgggcgt tcccccatgc cgctcgcctg gttcatggtg acgagcttcc actgctcctc
      421 ggcgctgcgc cccgcctcct gcgccaccag cgccagcagg tcctcgtcga aaatgtcggc
      481 cttcttgtcg gcgaggtcct tgaagcgctg gaacgccttg ttgtaggcgt cctcgctctc
      541 gagctcgacg ccgagctcct tcatgcgctg cctgaaggcg ctgcggcccg agagcttgcc
      601 cagcacgatc ttgttggcgg cccagccgac gtcctcggcg cgcatgatct cgtaggtctg
      661 gcgcgccttg agcacgccgt cctggtggat gcccgaggca tgcgcaaagg cgttggcgcc
      721 gaccaccgcc ttgttcggct gcaccacgaa gccggtgatc tgcgccacca ggcgcgacgc
      781 cggcacgatg tgcgtggtat cgatgcctgt tttcaggttg aaaaaatcac cgcgcgtctt
      841 gacggccatc accacctcct cgagcgaggt gttgccggcg cgctcgccga ggccgttgat
      901 ggtgcactcg atctggcgcg cgccgccgat catcaccgcg gcgagcgagt tggcgaccgc
      961 catgccgagg tcgttgtggc aatgcacgct ccacacggcc cggtccgagt tgggtatctt
     1021 ttctctcaat tgcttcatga attcgccgaa ttgctgcggc accgcatagc ccacggtgtc
     1081 ggggatgttg atggtggtcg cgccttccct gatcgccgcc tcgagcacgc ggcaaagaaa
     1141 gtccggatcc gagcggctgc cgtcttcggg ggagaactcg acgtcgggac aggcctgctt
     1201 cgcgtagcgc accgccagct tcgccgcttc cagcacctgc tgcggcgtca tgcgcagctt
     1261 cttctccatg tgcagcgcgc tggtggcgat gaaggtatgg atgcgccagg gccccctggc
     1321 ccccttcagc gcgtcgatgc cgcgctggat gtcgcggtcg ttcgcccggc acagcccgca
     1381 gaccgtcgac tcccggatca cgctcgcgac cgcgcgcacc gcctcgaagt cgccgttcga
     1441 ggacgccggg aaccccgcct cgatgacgtc gacccgcatg cgctccagcg cccgcgcgat
     1501 gcgcagcttc tcgtcccggg tcatcgacgc gccggggctc tgctcgccgt cgcgcatggt
     1561 ggtgtcgaaa atgatcaaac ggtctttgtt catggaataa atcctcggcc ctattgggcc
     1621 agtcgcagca cggattgtag gcggaggtat ttagcgcgcg gggcgcagca gcaggccagc
     1681 cagcaggacg aggctggtaa gcgacgcgat gagaagggca gtcgcggaaa gcatggccgg
     1741 actatagccc gaaagacggg gctggatcaa cctccgcctt tcggccgttg ccagcagccg
     1801 ctccaacgtc gctcgcgatc acgggatggt cgctaccaag cgccgttcaa agctcgagcg
     1861 cgagacgcaa accctcgtca agctgttgct gctgcgccgg cggcaggcgc ccgattcgtt
     1921 ccgtgagtac ggaccggtcc agcgtgatga gctgggaaac gttcgctacc gagtctcgct
     1981 tgagccgggt tgaacgcgcc cgcagcagca cgttcccggg ggcttcggca aggcgcagat
     2041 tggaagtaat caccgcgacg atcgcggtac gaatgcggct ggcattgaag ctgtcagcct
     2101 ggacgatcac taccggtcgg cggaatcccg ggccggagcc cgttggttcg ggcaacgatg
     2161 cccaccagat ttcgccgcgg cgcacttacc agcgctcgcg cgcaatcgac cggagctgtg
     2221 cagccgcaag cccagcctcg acgagcgagg cgttcgcgtc ggcgtaaacc tcattcagct
     2281 tcgcggtgac gctcgagtcg cggttgcgct ccaaataagc ggcaactgcg gccgcataca
     2341 actgactccg gctaagcttc agacgcttcg cagcgcgctc cgcagactgg aagataggat
     2401 cgggaatcga aattgccgtt ttcatccgtc gagtataact cgagttatac cttggcgcaa
     2461 cgctgctttc cggcgtgcgg cctattgcca gtttgcggcg atgacgggct ccagcgcggc
     2521 ggcgtagtcg ggcgacaatg aggtgaacgg cccatagacg gaacacttcc cttccggcag
     2581 agtcgaatcc acatgaagac actgctggcg gcagcttgcc tggcgcttgc ttcggcagcc
     2641 gcaaacgccg gcgaaaccat caacatctac aagtatcgcg gcgccgacgg ccgcccgctc
     2701 tacagcgatc gccccattcc gggggccaga ctgatcgaat cgatccaata cgttctcccc
     2761 gccccttcat cgcctcgctc cgatacctcc aagagcggcg ccgcgggcga ggaacgaatc
     2821 aagaaccacc tgtcggagct ggacgcagcc tgggccgagg tgaaggcatc cggcagtgcg
     2881 ctcgccgcgg cggaggcgcg ccttgccgcc ggcgtcacgc ccggggaagg cgagccgcgc
     2941 gcccttgccg gtccgccgac tccggcgccg ccctcggcgg ggggaccgat ggcgaccgcg
     3001 cctgcgccgg cggggggacc gctgccgc
//
LOCUS       DASPXW010000330         2425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1608314, whole genome shotgun sequence.
ACCESSION   DASPXW010000330 DASPXW010000000
VERSION     DASPXW010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1608314"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..862)
                     /locus_tag="VEL04_07505"
     CDS             complement(50..862)
                     /locus_tag="VEL04_07505"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HYT48249.1"
                     /translation="MNPNPGEQQYLDLLAEVLGRGARKSDRTGTGTLSVFGRQLRFAL
                     GESFPLLTTKKLHLKSILYELLWFLRGDTNVKWLQERGVSIWDEWADARGELGPVYGY
                     QWRHWRSADGREIDQIAQVIESLRRKPDSRRHIVTAWNPADVERMALPPCHALFQFYV
                     AEGRLSCQMYQRSADLFLGVPFNIASYAALTLMVAQVTGLEPGEFVLTLGDAHLYLNH
                     LEQARNQLTRAPRPFPRMRLNPAVKELLDFKYEDFTLEAYDPHPAIKAPIAV"
     gene            complement(859..1647)
                     /locus_tag="VEL04_07510"
     CDS             complement(859..1647)
                     /locus_tag="VEL04_07510"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HYT48250.1"
                     /translation="MAEALVPGYRVVHPIGEGGQAKVYLAEREHDGLRVALKVLDRIL
                     RQDRVFLERFVREYKLLSSLNNEYVARIYDQGFSGEQPYIAMEFLPSGTLAGRIVEGL
                     SARAALRLVLQLARALDAIHSHGIVHRDLKPSNILFRADGRPVIVDFGLAKDLAANST
                     LTVTGKLLATPRYMSPEQCLGQAVDTRSDLYSLGVVFYEMLTGKRIYDSARVAELIYL
                     HVSEPPPRLPEPLAEHQPLLDRMLAKQPEDRFQSAAELCAALMT"
     gene            1704..>2425
                     /locus_tag="VEL04_07515"
     CDS             1704..>2425
                     /locus_tag="VEL04_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48251.1"
                     /translation="MLNLFSGKADHPMADPKEARRILEQLPAQELKALDELAHWHESV
                     SLADGFKPAERLQLLTGIDDAAQPRVRKVARDYFAATRPSRYQENLMWTQLHEYWRQS
                     GLAWARAIDALLQTGKGGDAKALPAAAVRALRSLGQQIKWQHLRYGPIDAAVWGVINR
                     IYAAAESRGIAEARLEFLKIAMFSASSPDGLLPFELELAERLIGDFAAGFALAAAPAR
                     ELLYWTDLGRPMAPARATKKPPA"
BASE COUNT          391 a          786 c          858 g          390 t
ORIGIN      
        1 tgctgaacaa gggcgggcgc ggtgtgagca gcccgctcga ccgcctcgcc tagaccgcga
       61 tcggcgcctt gatcgcggga tgggggtcgt aggcctcgag ggtgaagtcc tcgtatttga
      121 agtcgaggag ttctttcacc gccggattaa gccgcatgcg cgggaacggg cgaggtgcgc
      181 gcgtcaattg gtttctggct tgttccaaat gattcaaata aagatgcgca tcgccgagtg
      241 tcaggacgaa ctcgccgggc tccaaacccg tcacctgcgc gaccatcagc gtgagagccg
      301 cgtaggaggc gatgttgaac ggcacgccga ggaacaaatc cgcgctcctc tggtacatct
      361 ggcaggagag cctgccctcg gcgacgtaga actggaacag ggcatggcag ggcggcagcg
      421 ccatgcgctc gacgtcggcg ggattccatg ccgtgacgat gtggcggcgc gagtcgggtt
      481 tcctgcgcag ggactcgatg acctgcgcga tctggtcgat ctcgcgcccg tcggcgctgc
      541 gccagtgccg ccactggtag ccgtacaccg gcccgagctc gccgcgcgcg tcggcccatt
      601 cgtcccagat gctgacgccg cgctcctgga gccacttgac gttggtatcg ccgcgcagga
      661 accagagcag ctcgtagagg atcgatttga ggtgcagctt cttggtggtg aggagcggaa
      721 agctctcgcc gagcgcaaag cgcagctgcc tgccgaacac cgagagcgtg ccggtgccgg
      781 tgcggtcgga cttgcgcgcg ccgcggccga gcacctcggc gagcaggtcg aggtactgct
      841 gctcgccggg gttcgggttc atgtcatcag cgccgcgcac agctcggctg cgctctggaa
      901 gcggtcctcg ggctgtttcg cgagcatccg gtcgagcagc ggctggtgct ccgcgagcgg
      961 ctcgggcagg cggggtggcg gctcgctcac gtgcaggtag atgagctcgg cgacgcgcgc
     1021 ggagtcgtag atcctcttcc cggtgagcat ctcgtagaac actaccccca ggctatagag
     1081 gtcggagcgc gtgtcgaccg cctgcccgag gcactgctcg gggctcatgt agcgcggcgt
     1141 ggcgagcagc ttgcccgtga cggtgagggt cgaattggcg gcgagatcct tggccaggcc
     1201 gaaatcgacg atcaccggcc ggccgtcggc gcggaacagg atgttcgagg gcttgaggtc
     1261 gcggtgcacg atgccgtgcg agtggatggc gtccagcgcg cgcgcgagct gcagcaccag
     1321 gcgcagcgcg gcgcgcgcgc tcagcccctc gacgatgcgg ccggcgagcg tgcccgaggg
     1381 caggaattcc atcgcgatgt agggctgctc gcccgagaac ccctggtcgt agatgcgcgc
     1441 cacgtactcg ttgttgaggc tggaaagcag cttgtactcg cgcacgaagc gctcgaggaa
     1501 cacgcggtcc tggcgcagga tgcgatcgag cactttcaag gcgacccgca gcccgtcgtg
     1561 ctcgcgctcg gcgagataga ccttcgcctg cccgccctcg ccgatcgggt gcaccacgcg
     1621 gtatccggga accagcgctt ccgccatgtg ccgtattctg ccatccgcgt gctacgattc
     1681 cccggggaat cgcgggggaa gcgatgttga atctgttcag cggcaaggcc gaccatccga
     1741 tggccgaccc gaaggaagcg cgccgcatac tcgagcagtt gccggcacag gaactgaagg
     1801 cgctcgacga gctggcgcac tggcacgaat cggtcagcct cgccgacggc ttcaagccgg
     1861 ccgagcgcct gcagcttctc acgggcatcg acgacgcggc gcagccgcgc gtgcgcaagg
     1921 tcgcgcgcga ttatttcgcc gccacgcggc cgtcgcgcta ccaggaaaac ctgatgtgga
     1981 cccagctcca cgagtactgg cgccagtcgg gccttgcctg ggcgcgcgcg atcgacgcgc
     2041 tgctgcaaac cggcaagggg ggcgacgcga aggcgctgcc cgccgccgcg gtgcgcgcgc
     2101 tgcgctcgct cggccagcag atcaagtggc agcacctgcg ctacggcccg atcgacgccg
     2161 cggtgtgggg cgtcatcaac cggatttacg cggcagccga gtcgcgcggc atcgccgaag
     2221 cgagattgga atttctcaag atcgccatgt tcagcgcgag ctcgccggat ggcttgctgc
     2281 cgttcgaact cgagctcgcc gagcggttga tcggcgactt cgccgctggc tttgcgctcg
     2341 cggccgcgcc ggcgcgcgag ctgctttact ggaccgatct cggccggccg atggcgcccg
     2401 cgcgcgccac caagaagccg ccggc
//
LOCUS       DASPXW010000331         2960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1107266, whole genome shotgun sequence.
ACCESSION   DASPXW010000331 DASPXW010000000
VERSION     DASPXW010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1107266"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="VEL04_07520"
     CDS             <1..837
                     /locus_tag="VEL04_07520"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYT48252.1"
                     /translation="ASFEEILSDKKIEAVVNTTPNPVHLETTLAAAKAGKHIFLDKPI
                     ANTIAEARAITHACRKAKVVLALGYQRRREAHFRWIRKEIERGQFGTLVNAEANISRD
                     RLGKIDLGSWRYTAEGMPGGVMLQIGIHYTDVLEYLLGPVKAVSGRFVRLVLPGDNPD
                     VASLVLEHENGALSTLNASYASASEYYLMNIYGKEASAYYDFHQGLRWLRRGSEKAKR
                     LSIKKNDPIVEELEEFAAAVRGDARPEMDGERGTASLAVILAGIKSAKEGRRVEVKEM
                     LG"
     gene            834..1775
                     /locus_tag="VEL04_07525"
     CDS             834..1775
                     /locus_tag="VEL04_07525"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYT48253.1"
                     /translation="MRKWLLLLGWMTFPACGQEPFPQKGPIEITVLFPAGSSADVTAR
                     VLTDGMSKQLGQRVLVVNRPGAGGAIGYKYVAAQKPDGYALVWNSNSISTTFHSGQLP
                     FDYKAFDAVARVLVESVVVAVRADSPFRTLNDFISEARRKPKAISVGHSGIGSHTHIS
                     LASLAHTSGVEVNEVPFGAAQVVPSLLGGHVDAVVQLPAALAAPVRQGQVRLLAVLIP
                     ERDPALAGVPTAREQGFDVSVEAWRGIAVPHGTPRPTIAALETSIRKTVESNEFVKAS
                     ENLGVRPAFLPANAFSELIAKEDASLSRLMLLIGLKK"
     gene            complement(1756..2232)
                     /locus_tag="VEL04_07530"
     CDS             complement(1756..2232)
                     /locus_tag="VEL04_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HYT48254.1"
                     /translation="MKMRITILVGTMTGTAQLCAQEMELALDDGDTRVETLLMDNLDS
                     SVFSRQGVFLICTSTYGQGDVPDNARALYEHLKAQRPNLSQVRYGVFGLGDRTYAETF
                     NFGGKRFDDILTELGATRIGERYKHDASSGVLPEETALEWCRQWLGLIAEATSSSR"
     gene            2297..>2960
                     /locus_tag="VEL04_07535"
     CDS             2297..>2960
                     /locus_tag="VEL04_07535"
                     /inference="COORDINATES: protein motif:HMM:NF006015.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HYT48255.1"
                     /translation="MNAPLTSAQQEAAYLSQLGARVRVWRDQHGMTRKALSAACGVSE
                     RYLAQLEAGRGNISVLLLRRVARAIGLPVEQLAREDEAAARGQRIALIGLRGAGKSTL
                     GAKLSESMKIPFVELDREVEKEAGAKLGEVFAMYGQDAFRRFERRALERVLAQHERAV
                     IAAGGGLVTDASSYKLLRERCLCVWLKAAPEDHMGRVIAQGDMRPFKGRAAALDEIGQ
                     LLA"
BASE COUNT          529 a          948 c          982 g          501 t
ORIGIN      
        1 gcctcgttcg aagagattct ttcggacaag aaaatcgaag cggtcgtcaa caccaccccg
       61 aacccggtgc acctggaaac cacgctcgcc gctgcgaaag cgggaaagca cattttcctc
      121 gacaagccga tcgccaacac catcgccgag gcccgcgcca tcacgcacgc ctgccgcaag
      181 gcgaaggtgg tgctcgcttt ggggtatcag cgtcgccgcg aagcgcattt cagatggatc
      241 aggaaggaaa ttgaaagagg acagttcggc acactggtca acgccgaagc caacatcagc
      301 cgcgaccgcc tcggcaagat cgacctcggt tcctggcgct atacggccga ggggatgccc
      361 ggcggcgtga tgctgcagat cggcatccac tacaccgatg tcctcgaata cctgctcggg
      421 ccggtgaaag cggtgagcgg gcggttcgtg cgcctcgtgc tgccgggcga caacccggac
      481 gtcgcgagcc tggtgctcga gcacgagaac ggcgcgctct cgacgctcaa cgcgagctat
      541 gcctcggcct ccgagtacta cctgatgaac atctacggca aggaggcgag cgcgtactac
      601 gattttcacc agggtctaag gtggttgcgg cgcggatccg aaaaagccaa acgcctttcc
      661 atcaagaaga atgacccgat cgtcgaggag ctcgaggaat tcgccgccgc ggtgcggggg
      721 gatgccaggc cggagatgga cggcgagcgc ggcactgctt ctctcgcggt gatcctcgcc
      781 ggcatcaagt ccgcgaagga aggccgcagg gtcgaagtga aggagatgct gggatgagaa
      841 agtggttgct gctcctcggc tggatgactt ttcccgcctg cggccaggaa ccttttccgc
      901 aaaaaggccc gatcgagatc accgtgctat tcccggcggg cagctcggcc gacgtcaccg
      961 cgcgcgtgct caccgacggc atgtcgaagc agctcggcca gcgcgtgctg gtggtgaacc
     1021 gccccggcgc gggcggagcg atcggctaca aatacgtcgc ggcgcagaag cccgacggct
     1081 acgcgctggt atggaattcg aactcgatct ccaccacctt tcactcgggc cagctgccgt
     1141 tcgactacaa ggctttcgat gcagtggcgc gcgtgctggt cgagtcggtc gtggtcgcgg
     1201 tgcgcgcgga ttcgcctttc agaactttga acgatttcat ttctgaagcg cgcagaaagc
     1261 ccaaggccat cagcgtcggc cattcgggga tcggcagcca tacgcatatc tcgctcgcca
     1321 gcctggcgca cacctcgggc gtggaagtga acgaagtgcc gttcggcgcc gcgcaggtgg
     1381 tgccgagcct gctcggcgga cacgtcgacg ccgtggtgca gctgcctgcc gcgctcgcgg
     1441 cgccggtcag gcagggccag gtgcggctgc tcgccgtcct catccccgag cgcgacccgg
     1501 cgctcgccgg tgtgccgacg gcgcgcgagc agggcttcga tgtctcggtc gaagcctggc
     1561 gaggcatcgc cgtgccgcat ggcacgccgc ggccaaccat tgctgccctg gaaacttcaa
     1621 taagaaaaac cgttgagtcg aatgaattcg tgaaggcaag tgaaaacctt ggagtgcgcc
     1681 cggcgttcct gccggccaac gccttcagcg agctgatcgc gaaggaggac gcttcgctgt
     1741 cgcgcctgat gctgcttatc ggcttgaaga agtagcttcg gcgatcaacc cgagccattg
     1801 ccggcaccac tcgagcgcgg tttcctcggg aagcacgccg ctggaagcgt cgtgcttgta
     1861 gcgctcgccg atgcgcgtcg cgccgagctc ggtcaggatg tcgtcgaaac gcttgccgcc
     1921 gaaattgaag gtctcggcgt aggtcctgtc gccgagcccg aacacgccat agcgcacctg
     1981 cgacaggttc ggccgctgcg ccttgagatg ctcgtacagg gcgcgcgcgt tgtccggcac
     2041 gtcgccctgt ccgtaggtgg aggtgcagat caggaacacc ccctggcgcg agaacaccga
     2101 gctgtcgagg ttgtccatga gcagggtctc gacccgggtg tcgccgtcgt cgagcgccag
     2161 ctccatttcc tgcgcgcaga gctgcgcggt tccggtcatg gtgccgacca gaatggtgat
     2221 ccgcattttc attgacctga aaatatattg cacactataa ttcgtgtcaa gcactatagt
     2281 tcatgctcat ttctccatga atgcgcctct cacctccgcc cagcaggaag ccgcctatct
     2341 ttcccagctt ggcgcgcgcg tgcgcgtgtg gcgcgaccag cacggcatga cgcgcaaggc
     2401 gctgtccgcc gcgtgcggag tttccgagcg ctatctggcg cagctcgaag cggggcgggg
     2461 aaacatctcc gtgctgctgc tgcgccgggt ggcgcgggcg atcggcttgc ccgtcgagca
     2521 gctcgcgcgc gaggacgagg cagccgcgcg cggccagcgc atcgcgctca tcggcctgcg
     2581 cggtgcgggc aagtcgacgc tcggggcgaa gctgtccgag tccatgaaaa tccccttcgt
     2641 cgagctcgac cgcgaggtcg agaaggaagc cggcgccaag ctcggcgagg tgttcgcgat
     2701 gtacggccag gacgcgttcc ggcgcttcga gcgccgcgcg ctcgagcgcg tgctcgcgca
     2761 gcacgagcgc gccgtgatcg ccgccggcgg cggcctcgtc accgatgcct cgagctacaa
     2821 gctgctgcgc gagcgctgcc tgtgcgtatg gctcaaggcc gcgcccgagg accacatggg
     2881 gcgggtcatc gcccagggcg acatgcggcc gttcaaagga cgcgcggcgg cgctcgacga
     2941 gatcgggcag ctgcttgccg
//
LOCUS       DASPXW010000332         3241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_348803, whole genome shotgun sequence.
ACCESSION   DASPXW010000332 DASPXW010000000
VERSION     DASPXW010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348803"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="VEL04_07540"
     CDS             complement(<1..574)
                     /locus_tag="VEL04_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYT48256.1"
                     /translation="MLYREAGQFKSTYAEDQQIFPIRQDRIAVGLMLVVAFVLVPLFG
                     NQYWLGALLTPFLIFALATLGLNILTGYAGQLSLGTAAFMAVGAFMAYNFVLRMPWLG
                     VIPSFILAGLCAAAVGIVFGLPSLRIKGFYLAVATLACQFFVLWAIQRWGWITNYSTS
                     GVITAQKIVILGYAFDTPASKYLLTLAVVAV"
     gene            complement(592..1491)
                     /locus_tag="VEL04_07545"
     CDS             complement(592..1491)
                     /locus_tag="VEL04_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYT48257.1"
                     /translation="MEFVFFIEVLAGGLLAGVMYALVALGFVLIYKASGVFNFAQGSM
                     VFFAALTFVSLTERGWNFWLALVATLAVMVLLGIVTERVVLRPLVNQPPITLFMATIG
                     LTFVLEGFSQLIWGSQPHGLELGIPDVPMEWLSQKWNMNISQFDLFAAFVAGLLVTVL
                     ALLFQKTRIGRALRAVADDHQAALAVGIPLQHIWAIVWAVAGFVALVAGLMWGVRNGV
                     QYALTFVALKALPVLILGGFESIQGAIVGGLIIGATEKLAEVYIGPLVGGGIESWFAY
                     VVALLFLLVRPEGLFGEKHIDRV"
     gene            complement(1492..2280)
                     /locus_tag="VEL04_07550"
     CDS             complement(1492..2280)
                     /locus_tag="VEL04_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020480182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT48258.1"
                     /translation="MSAGRSIGDTVLSVEGVSLAFGGVRALQDVSFDVRSREVRAIIG
                     PNGAGKSSMLNVLNGVYHPQSGRIRFKNKEFSDMDSHEAATLGIARTFQNIALFKGMT
                     VLDNLMTGRNLKMRCNFLQQALWIGPARREELEHRRAVEEIIDFLEIQHIRKTPVGRL
                     PYGLQKRVELARALAAEPTLLLLDEPMAGMNLEEKQDMCRFVLEVNDHFGTTLVLIEH
                     DMGVVMDISDRVVVLDYGRKIADGSPEEVRSNQKVIDAYLGVAH"
     gene            complement(2277..>3241)
                     /locus_tag="VEL04_07555"
     CDS             complement(2277..>3241)
                     /locus_tag="VEL04_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYT48259.1"
                     /translation="YWIGDFVVYGPLRNVLGMSRVRVAYTAGEAIGPDLFKFYRAIGI
                     NLKQLYGSTETSVFVCIQPDGQVKADTAGPPVAGVELKFTPQRELLIRSPGLFKEYYK
                     NPQATREAKDAEGWFHTGDAGYFDADGHLKIIDRVKDVGKLADGTLFAPKYLENKLKF
                     FPYIKEAVCFGDRRDRVCAFINIDPDAVANWAEKRNMPYSGYTDLASRDEVYDLVREC
                     IARVNADLSAEPEIANSQIHRFLILHKELDADDGELTRTRKVRRGFIGEKYGSLVDAL
                     YDNQQSVHVQAQVKYEDGRSGTFSADLKIRDAKTFAPAASRRAA"
BASE COUNT          592 a         1064 c         1042 g          543 t
ORIGIN      
        1 tcaccgcgac caccgcgagc gtcagcaggt atttgctcgc cggcgtgtcg aacgcgtagc
       61 cgaggatcac gatcttctgt gccgtgatca cacccgaggt gctgtaattg gtgatccagc
      121 cccagcgctg gatcgcccac agcacgaaga actggcaggc gagcgtcgcc accgcgaggt
      181 agaagccttt gatgcgcagg ctcggcaggc cgaacacgat tccgaccgcc gccgcgcaca
      241 gcccggcgag gatgaagctc gggatcactc cgagccacgg catgcgcagc acgaagttgt
      301 aggccatgaa tgcgcccacc gccatgaatg ccgcagtgcc aagcgacagc tgcccggcat
      361 agccggtgag gatgttcagg ccgagcgtgg caagggcaaa gatcagaaaa ggcgtcagca
      421 gcgcgcccag ccagtactgg ttgccgaaca ggggcaccag cacgaaggcc acgacgagca
      481 tcaggcccac cgcgatccgg tcctgccgga tcggaaaaat ctgctggtct tcggcgtagg
      541 tggatttgaa ttgcccggct tcgcggtaga gcatcgctgc ctttttctcc cctagacccg
      601 gtcgatgtgc ttttcgccga acagcccctc gggccgcacc agcaggaaga gcagcgccac
      661 cacgtaggcg aaccagctct cgatgccgcc gccgaccagc gggccgatgt agacctcggc
      721 gagcttctcg gtggcgccga tgatcaagcc gccgacgatc gcgccctgga tcgactcgaa
      781 gccgccgagg atcagcaccg ggagcgcttt cagcgcgacg aaggtgagcg cgtactgcac
      841 gccattgcgc accccccaca tcaggccggc gaccagcgcc acgaaccccg ccaccgccca
      901 cacgatcgcc cagatgtgct gcagcgggat gccgacggcg agcgccgcct ggtggtcgtc
      961 ggccaccgcg cgcagcgcgc ggccgatgcg cgtcttctgg aacagcagcg ccagcaccgt
     1021 gaccagcagc ccggcgacga aagcggcgaa caggtcgaac tggctgatgt tcatgttcca
     1081 cttctgcgac agccattcca tcggcacgtc ggggatgccg agctccaggc cgtgcggctg
     1141 cgagccccag atgagctgcg agaacccctc gaggacgaag gtcaggccga tggtggccat
     1201 gaacagcgtg atcggcggct ggttgacgag cggccgcagc accacgcgct cggtgacgat
     1261 gccaaggagc accatcacgg cgagcgtcgc cacgagcgca agccagaaat tccagccgcg
     1321 ctcggtgagc gagacaaagg taagcgccgc aaaaaacacc atcgacccct gcgcgaagtt
     1381 gaatacgccc gaggccttgt agatcagcac gaagccgagc gcgaccagcg catacatcac
     1441 gcccgcgagc aggccgccgg ccagcacttc gatgaaaaac acgaattcca tctagtgcgc
     1501 gactcccagg taggcgtcga tgaccttctg gttgcttctt acttcttcgg gcgagccatc
     1561 cgcgatcttg cgcccgtagt ccagcaccac cacgcgatcg gagatgtcca tcaccacgcc
     1621 catgtcgtgc tcgatcagca ccagcgtggt gccgaagtgg tcgttgacct caagcacgaa
     1681 gcggcacatg tcctgctttt cctctaggtt catgccggcc atcggctcgt cgagcagcag
     1741 cagcgtcggc tcggcggcga gcgcgcgggc gagctcgacg cgcttctgca ggccgtaagg
     1801 cagtcgcccc accggggtct tgcggatgtg ctggatctcg aggaagtcga tgatctcctc
     1861 gaccgcgcgg cggtgctcga gctcctcgcg ccgggccggg ccgatccaca gcgcctgctg
     1921 caggaagttg cagcgcatct tgaggttgcg gccggtcatg aggttgtcga gcaccgtcat
     1981 gcccttgaac agcgcgatgt tctggaaggt gcgcgcgatg ccgagcgtcg cggcctcgtg
     2041 cgagtccatg tcggaaaatt ccttattttt gaaacgaatc cttcctgact gcgggtgata
     2101 cacgccgttc aggacattga gcatcgagct cttgccggcg ccattggggc cgatgatcgc
     2161 gcgcacctcg cggctgcgca cgtcgaagga gacgtcctgc agcgcgcgca cgcccccgaa
     2221 ggcgagcgac acgccctcga ccgacagcac cgtgtcgccg atggacctgc cggcgctcac
     2281 gcggctcgcc gggacgcggc aggcgcgaag gtcttcgcgt cgcggatctt gaggtcggcg
     2341 gagaacgtgc cgctgcggcc gtcctcgtat ttcacctgcg cctgcacgtg cacgctttgt
     2401 tgattgtcat atagggcatc aacgagcgag ccgtatttct cgccgatgaa gccgcggcgc
     2461 accttgcgcg tgcgggtcag ctcgccgtcg tcggcatcga gctccttgtg caggatcagg
     2521 aacctgtgga tctgggaatt cgcaatctcc ggctcggccg acaggtcggc attcacccgc
     2581 gcgatgcatt cgcgcaccag gtcgtacacc tcgtcgcgcg aggcgaggtc ggtgtagccc
     2641 gagtacggca tgttgcgctt ctcggcccag ttggcgacgg cgtccggatc gatgttgatg
     2701 aatgcgcaca ccctgtcgcg gcggtcgccg aagcacacgg cctccttgat gtaggggaag
     2761 aacttgagct tgttctcgag gtatttcggc gcgaacagcg tgccgtcggc gagcttgccg
     2821 acgtctttca cccggtcgat gatcttgagg tggccgtcgg cgtcgaaata gccggcgtcg
     2881 ccggtgtgga accagccttc ggcgtccttt gcctcgcgcg tcgcctgcgg gttcttgtag
     2941 tactccttga acagcccggg cgagcggatc agcagctcgc gctggggcgt gaacttgagc
     3001 tcgacccccg cgaccggcgg ccccgcggtg tcggccttga cctggccgtc gggctggatg
     3061 cagacgaaca cgctggtttc ggtcgacccg tagagctgct tcaggttgat gccgatggca
     3121 cggtagaact tgaataggtc aggcccgatc gcctcccccg cggtatacgc gacgcgcacc
     3181 cggctcatgc cgaggacatt gcgcagcggc ccgtagacga cgaagtcgcc gatccaataa
     3241 a
//
LOCUS       DASPXW010000333         4300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1860664, whole genome shotgun sequence.
ACCESSION   DASPXW010000333 DASPXW010000000
VERSION     DASPXW010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1860664"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="VEL04_07560"
     CDS             <1..734
                     /locus_tag="VEL04_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007584596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HYT48260.1"
                     /translation="ALIGADGVHSSIRQTLHGSDRPEFTGTIAWRGVVPMERLPRHMA
                     RMVGSNWVGPGGHIVHYPLRAGKLMNFVGVLERSDWKVESWSTRGSTEELAGDFRGWH
                     ADIQAMIRNIDTPYKWALMVRAPMERWSAGRVILLGDACHSMLPMLAQGAVMAIEDGY
                     VLARCFAQAGSDVPAALERYENARRERTRRVVEGSAANARRFHNPKLADPAEAKRYVD
                     REWAEARVAERYEWLFRYDVTQVQI"
     gene            734..2197
                     /locus_tag="VEL04_07565"
     CDS             734..2197
                     /locus_tag="VEL04_07565"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007733837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HYT48261.1"
                     /translation="MDFRLRGFLESLGPDELQRIDSAVELADVAAALEGNRKAVWFMR
                     AGTEGASLAGNVAASRSRLAHAFGTTPEGLLQEVLRRLKNEGEIIESKAAPVHEVIER
                     EPDLTVLPVHLQHGLDGAPYISAAIDFALDAERGVTNVGMRRLMLRGRREAGVDLNAP
                     SDLRAIYQAQVKVGKRLPIAFAVGSHPIDQVAATMRLPGDELALIGALRGAPLPVVKC
                     VTSDLRVPADSEYVLEGYLDERGYAEPEGPYGEFLGYYGAVKTNPVFHLTAVARRRDA
                     VFQTSTISGRTLARTDTAQLNALRAEVSVWRALETAVRGVRAVFATPASGGAFNVRVA
                     LQSRVPGEARNAIAAVFGCLVNVKHVFVVDPDVDIFSDEQMEWALATRFQADRDLVVQ
                     SGFRSVPLDPSLGDSMTGAKAGFDMTSDAKVLVPQPPRYQGRRFASVEAALADGPKFF
                     AELMAARASRDGREIVRALEALRERLGRDDEGRYLLK"
     gene            2194..3144
                     /locus_tag="VEL04_07570"
     CDS             2194..3144
                     /locus_tag="VEL04_07570"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYT48262.1"
                     /translation="MNRIVWPFLALFSAATLAQEPSWPSKPVKWLVPFAPGGPADVVT
                     RIVAAGLAERIGQPNVVENQPGASGNIAHAAASKAPPDGHTLVFVVPAVITNPFYLKV
                     AADPFRDLAPVIHLDNASMVLIANPAFPPATLSEVISFVKAHPGRVTCGASGALPSVG
                     CELLRAHAGADVLMVMYKGNAPALNALIGGEIHLLFDVVNIAVGHVRSGRVRAIASTA
                     PRRGIGPLGELPVMAEAIPGFELVTWHGVMISPATPRGIVERVNRELNAVLENPDVRQ
                     RFANSGLQITGGPPEDFAAVLRHDYDKYGSALRAAGVKPE"
     gene            3141..3584
                     /locus_tag="VEL04_07575"
     CDS             3141..3584
                     /locus_tag="VEL04_07575"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYT48263.1"
                     /translation="MTRRVITGHTAGKAVILHDAPAPNVKVRPGSGLVSTLLWVTDES
                     PAELAGSADRASREIGIPPPRNGSIFRVVDFPVGAKGAMHRTRSVDYAVVLEGEIDLL
                     LPDADVRLRAGDVLVQQGTSHAWANRGGGRCRIAFVLIDAKSSEA"
     gene            3615..4019
                     /locus_tag="VEL04_07580"
     CDS             3615..4019
                     /locus_tag="VEL04_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HYT48264.1"
                     /translation="MTAAAPNPMVPLAARILLGAIFLVSGIRKLMAYAGTVAYFTKLG
                     FPAPDAMAVIAIIIEVGGSILLIVGWRTRWVAWLLALFVLIAAFAAHRFWEFDAAQMN
                     NQMNHFLKNFAIIGGMFYVATFGAGRYSIDKA"
     gene            4006..>4300
                     /locus_tag="VEL04_07585"
     CDS             4006..>4300
                     /locus_tag="VEL04_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48265.1"
                     /translation="MTKLEKTPGRADVALIRALERVNIHPLWDRYQRITPLAPKPQDA
                     PMHWRWRDIAPFAERAAKEVGIEDVERRALILANPAFGGATVTTQNLIGAFTVL"
BASE COUNT          684 a         1451 c         1475 g          690 t
ORIGIN      
        1 atgcgctcat cggtgccgac ggcgtgcact cgagcattcg ccagacgctg cacgggtccg
       61 accggcccga attcaccggc accatcgcgt ggcgcggcgt cgtgccgatg gagcggctgc
      121 cgcggcacat ggcgcgcatg gtcggctcga actgggtggg cccgggcggc cacatcgttc
      181 actacccgct gcgcgccggg aagctgatga acttcgtcgg cgtgctggag cgttccgact
      241 ggaaggtcga atcgtggagc acgcgcggca gcaccgagga gctcgccggc gacttcaggg
      301 ggtggcacgc cgacatccag gccatgatcc gcaatatcga cacgccctat aagtgggcgc
      361 tgatggtgcg cgcgccgatg gagcgctgga gcgccgggcg cgtgatcctg ctcggcgatg
      421 cctgccactc gatgctgccg atgctcgccc agggagcggt catggcgatc gaagacggct
      481 atgttctggc gcgctgcttc gcgcaagccg gaagcgatgt gccggcggcg ctcgagcgct
      541 atgaaaacgc gcgccgcgag cgcacgcgcc gggtggtcga aggctcggcg gccaacgcca
      601 ggcgcttcca caacccgaag ctcgccgacc cggccgaggc gaagcgctac gtcgaccgcg
      661 aatgggccga ggcgcgcgtc gccgagcgct acgaatggct gtttcgctat gacgtcacac
      721 aggtgcagat ctgatggact tcaggctgcg agggtttctc gaatcgctgg ggccggacga
      781 gctgcagcgc atcgacagcg cggtcgagct tgcggatgtc gccgcagcgc tcgaaggcaa
      841 ccgcaaggcg gtctggttca tgcgggctgg aaccgaaggc gcgtcgctcg ccggaaacgt
      901 cgcggcgagc cgctcgcgcc tcgcgcatgc cttcggcacg accccagagg ggttgctgca
      961 ggaagtgttg cgtcgcctga aaaacgaggg cgaaatcatc gaaagcaagg ctgcgccggt
     1021 gcatgaggtc atcgaaaggg agccggatct gactgtcctc ccggttcatc tgcagcacgg
     1081 cctggacggc gccccctaca tctccgccgc gatcgatttc gcgctcgacg cggagcgcgg
     1141 cgtcaccaac gtcggcatgc gccgcctgat gctgcgcggc cggcgcgagg cgggcgtcga
     1201 cctcaacgcg ccctccgacc tgcgcgcgat ctaccaggcg caggtgaagg tcggcaaacg
     1261 gctgccgatc gcattcgcgg tgggctcgca cccgatcgac caggtggcgg cgacgatgcg
     1321 ccttccgggg gacgagctgg ccctgatcgg cgcgctgcgc ggtgcgccgc tgccggtagt
     1381 gaagtgcgtg accagcgacc tgcgtgtccc ggccgactcc gagtacgtgc tcgagggcta
     1441 cctcgacgag cgcgggtatg ccgagcccga agggccttac ggcgagttcc tcggctatta
     1501 cggcgcggtg aagaccaatc ccgtgttcca cctgacggcg gtcgcgcgcc ggcgcgacgc
     1561 cgtgttccag acctccacca tcagcggccg cacgctggcg aggaccgaca cggcgcagct
     1621 caacgcgctg cgcgccgagg tcagcgtgtg gcgcgcgctc gagaccgcgg tgcgcggcgt
     1681 ccgggcggtc ttcgcgacgc cggcgagcgg cggcgccttc aacgtcagag tggcgctgca
     1741 gagccgcgtg ccgggcgagg cgcgcaacgc aatcgcggcg gtgttcggct gcctggtcaa
     1801 cgtgaagcac gtcttcgtgg tcgacccgga cgtcgatatc ttttccgacg agcagatgga
     1861 atgggcgctc gcgacgcgct tccaggccga tcgcgacctg gtggtgcagt ccggatttcg
     1921 cagcgtgccg ctcgaccctt cactcggcga cagcatgacg ggcgccaagg ccgggtttga
     1981 catgacgagc gacgccaagg tgctggtgcc gcagccgccg cgctaccagg gcaggcgctt
     2041 cgcttcggtc gaggcggcgc tcgccgacgg gcccaagttc ttcgccgagc tgatggcggc
     2101 gcgcgccagc cgcgacggcc gcgagatcgt gcgcgcgctg gaagcgctgc gcgagcggct
     2161 cggccgcgac gacgaaggca ggtacttgct gaaatgaacc gcatcgtttg gccttttctt
     2221 gcgctcttta gtgcagcaac cctcgcccaa gaaccatcct ggccgagcaa gcccgtcaaa
     2281 tggctcgtgc ccttcgcccc cggaggcccg gcggacgtgg tgacgcgcat cgtcgccgcc
     2341 gggctcgccg agcgtatcgg ccagccgaac gtggtagaga accagcccgg cgcgagcggc
     2401 aacatcgcgc atgccgcggc ctccaaggcc ccgcccgacg gccatacgct ggtgttcgtc
     2461 gtgccggcgg tgatcaccaa tccgttctac ctgaaggtcg cggccgatcc attccgcgat
     2521 ctcgcgccgg tgatccatct cgacaacgcg tcgatggtgc tgattgcaaa tccggcgttt
     2581 cctcctgcca ccttgagtga agtcatttct tttgttaaag cgcaccccgg ccgagtcacc
     2641 tgcggcgcct cgggcgcgct tccctcggtc ggctgcgagc tgctccgggc gcacgccggc
     2701 gccgacgtgc tgatggtgat gtacaagggc aacgcgccgg cgctgaacgc gctgatcgga
     2761 ggtgaaatcc atctgctctt tgacgtggtc aacatcgccg tcggccacgt caggtcggga
     2821 cgcgtgcgcg ccatcgcttc gaccgcgccg cgccgcggca tcggcccgct cggcgagctg
     2881 ccggtgatgg ccgaggccat tcccggcttc gagctggtca cctggcacgg cgtgatgatc
     2941 tcgcccgcga cgccgcgcgg tatcgtcgag cgggtgaatc gagagctcaa cgcggtgctc
     3001 gaaaatccag acgtacgcca gcgctttgcc aactcgggcc tgcagatcac cggcggcccg
     3061 ccggaggatt tcgccgcagt gctgcggcac gattacgaca aatatggcag cgcgctgcgc
     3121 gcggccgggg tcaagccgga atgacccggc gagtcatcac cgggcacacc gccggaaagg
     3181 ctgtcatcct gcacgacgcg ccggcgccca acgtgaaagt caggccgggc tcgggtctgg
     3241 tatcgacgct cctgtgggtg accgacgaat cgccggcgga gctggcgggc agcgccgatc
     3301 gcgcgtcgcg cgagatcggc attccgccgc cgcgcaacgg cagcatcttc cgtgtcgtcg
     3361 acttcccggt cggcgcaaaa ggcgcgatgc accgcacccg cagcgtcgac tacgcggtcg
     3421 tgctcgaggg cgagatcgac ctgctgttgc ccgacgccga cgtgcgcctg cgggcgggcg
     3481 acgtgctggt gcagcagggc actagccatg cctgggccaa tcgcggcggc ggcaggtgcc
     3541 gcatcgcctt cgtcctcatc gatgcaaaaa gctctgaggc ataatccgcg acacaaggag
     3601 ggctcatgac taccatgact gcagcggctc cgaatccgat ggttcctctc gcggcacgca
     3661 tccttctcgg cgccatattt ctcgtttccg gcatccgcaa gctgatggcg tacgcgggca
     3721 cggtggctta cttcaccaag ctgggattcc cggcgccgga tgccatggca gtgatcgcga
     3781 tcatcatcga ggtcggcggc tcgatcctgc tgatcgtcgg ctggcgcacg cgctgggtgg
     3841 cgtggctgct cgcgctgttc gtcctgatcg cagctttcgc cgcgcaccgc ttctgggaat
     3901 tcgacgccgc ccagatgaac aaccagatga accactttct gaagaacttc gccatcatcg
     3961 gcggcatgtt ttacgtcgcc accttcggcg ctggacggta ttcgattgac aaagcttgag
     4021 aaaactccgg ggcgagccga cgtcgcgctg atccgggcgc ttgagcgcgt caacatccac
     4081 ccgctctggg accgctatca gcgcatcacg ccgctcgcgc ccaagccgca agacgcgccg
     4141 atgcactggc gctggcgcga catcgcgccc ttcgccgagc gcgcagcgaa ggaggtcggc
     4201 atcgaggacg tggagcggcg cgccctgatc ctagccaacc cggcgttcgg cggtgccacg
     4261 gtgacgacgc agaacctgat cggcgctttc accgtgctcg
//
LOCUS       DASPXW010000334         2196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_222869, whole genome shotgun sequence.
ACCESSION   DASPXW010000334 DASPXW010000000
VERSION     DASPXW010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222869"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VEL04_07590"
     CDS             <1..807
                     /locus_tag="VEL04_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HYT48266.1"
                     /translation="DGIEFIEYATSQPQAFGDVLQKLGFAPVARHRSREVMLYRQGSM
                     NLIVNSHGATGAMPAVSAIALRVRDAAFAHKRSLDLGAWDMPTRASAMELNIPGIHGV
                     GDSLVYFVDRYRDFSIYDVDFVPMSGAGARPPAVAGLHYFGVVQAILGERTRDWVDFY
                     RNLFGFSVLPRGQYFGVLPKGTLLESPCHKFYLQLIEPPSGADDIHWDEGLVRIGLGA
                     PNVPAAVRALQERGVVFVDHGSVQPSDKGALTQFYLGGVTFEFVVSHLKP"
     gene            804..1682
                     /locus_tag="VEL04_07595"
     CDS             804..1682
                     /locus_tag="VEL04_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HYT48267.1"
                     /translation="MNLDHFGMDTITLAGPLEAKLRAVKAAGFTQIMLNATDIVGHPD
                     GEGAAIAAVRASGLRVTGFQVLRDFEGLSGHLHAYRVDVAKAMLDMCRALGSNVLLAC
                     SSTSAHAAGDPQLLTKDLQKLAMLAVPLNIRIAFEALSWGRYVNEFPQAWDIVQDADR
                     VNLGVCLDSYHILANRTDLDRLQDIDPHKVFLVQLSDFMWQEVRSPEDRIDTARHFRV
                     FPGEGVHNDPLAELVRKVDDMGYRGDYSFEVFNDDYRQLPLPMVCERAKRSVKWVSDL
                     VSRRSLPLRGAGALHG"
     gene            1679..>2196
                     /locus_tag="VEL04_07600"
     CDS             1679..>2196
                     /locus_tag="VEL04_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48268.1"
                     /translation="MNALAIYDKLTAGRTRFVRVDELCARAAEDFPGFLPSKNKMEAE
                     ARRALKDKQRLEIDQGLFLSQVLADAAAGAHLCHAMLLPHPGSSARLAEYERKGELDF
                     GGASLGRRGKASVLTMKNPDFLNAEDESTLEGMEQAVDVALLDPKTEVCVLRGAPVAH
                     PKYAGGRIFGAG"
BASE COUNT          366 a          724 c          718 g          388 t
ORIGIN      
        1 gacggcatcg agttcatcga gtacgccacg tcgcagccgc aggcgttcgg cgacgtgctg
       61 caaaaactgg ggttcgcgcc ggttgcacgc caccgctcgc gcgaggtgat gctgtatcgg
      121 cagggctcga tgaacctgat cgtcaattcg cacggcgcga ccggcgccat gcccgcggtg
      181 tcggcgatcg cgctgcgcgt acgcgacgcg gccttcgcgc acaagcgttc gctcgacctg
      241 ggcgcctggg acatgccgac gcgggcctct gcaatggagc tcaacatccc gggcatccac
      301 ggtgtgggcg acagcctcgt ctacttcgtc gatcgctacc gcgatttctc gatctacgac
      361 gtcgatttcg ttcccatgtc cggcgccggc gcccggccac cagcggtcgc aggcctgcac
      421 tacttcggcg tcgtgcaagc catcctgggc gagcgcacgc gcgactgggt ggacttctat
      481 cgcaatctct tcggcttctc ggtgctgccg cgcggccagt atttcggtgt gctgccgaaa
      541 ggcacgctgc tcgagagtcc ctgccacaaa ttctatttgc agctgattga gccgccgtcc
      601 ggcgccgacg acatccattg ggacgaaggc ctggtgcgca tcggcctggg cgcaccgaat
      661 gtcccggcgg cggtgcgcgc actgcaagag cgcggcgtgg tcttcgtcga ccatggctcg
      721 gtgcagccga gcgacaaggg cgcgttgacc cagttctacc tcggcggcgt gacgttcgaa
      781 ttcgtcgtca gccatctcaa accatgaatc tcgaccattt cggcatggac acgatcacgc
      841 tggcggggcc gctcgaggcc aagctgcgcg cggtaaaagc ggcgggcttc acccagatca
      901 tgctcaacgc gaccgacatc gtcggtcatc ccgacggtga gggcgcggcg atcgcggccg
      961 tacgtgcaag cggactgcgg gtcaccggct tccaggtatt gcgcgacttc gagggattgt
     1021 cgggccacct ccatgcctac agggtcgacg tcgccaaggc gatgctcgac atgtgccgtg
     1081 cgctgggctc gaacgtcctg ctcgcctgct cgtcgacttc cgcgcacgcc gccggcgatc
     1141 cgcagctatt aacgaaagac ctgcagaagc tggcgatgct ggcggtgccg ctcaacatcc
     1201 gtatcgcctt cgaggccctc tcctggggcc gctacgtcaa cgagtttccg caggcctggg
     1261 acatcgtcca ggacgccgac cgcgtcaacc tcggggtgtg cctcgactcg taccatatcc
     1321 tcgccaaccg caccgacctc gatcggcttc aggacatcga tccgcacaag gtgtttctcg
     1381 tgcagctgtc cgatttcatg tggcaggaag tgcgctcgcc cgaggaccgg atcgacaccg
     1441 cgcggcattt ccgcgtgttc ccgggcgaag gcgtccacaa cgacccgctc gccgagctgg
     1501 tgcgcaaagt ggacgacatg ggctatcgcg gggactacag tttcgaggtg ttcaacgacg
     1561 attaccgcca gctgccgctg ccgatggtct gcgagcgcgc caagcgctcg gtgaaatggg
     1621 tcagcgatct ggtgtcgcgg cgcagcctgc cgctgcgcgg cgccggtgca ttacacggat
     1681 gaacgctcta gcaatctacg acaagctgac cgcaggccgt acccgcttcg tacgcgtgga
     1741 cgagctgtgc gcgcgggcgg ccgaggactt tcctggtttt cttccatcaa aaaacaaaat
     1801 ggaggcggaa gcccggcgag cgctcaagga caagcagcgc ctggaaatcg accaggggct
     1861 gttcctgtcg caggtgcttg ccgatgccgc cgccggtgcg catctctgcc acgcgatgct
     1921 tctgcctcac ccgggctcga gcgcgcgcct ggcggagtat gagaggaaag gcgagctgga
     1981 tttcggcggc gccagcctcg ggcgccgcgg caaggcatcg gtgctcacga tgaagaaccc
     2041 ggacttcctc aacgccgagg acgagtcgac gctcgagggc atggagcagg cagtcgatgt
     2101 cgcgctgctc gacccgaaga ccgaggtctg cgtgctgcgc ggcgcgccgg tcgcgcatcc
     2161 caagtacgcg ggcgggcgga tcttcggcgc cggcat
//
LOCUS       DASPXW010000335         2364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1108813, whole genome shotgun sequence.
ACCESSION   DASPXW010000335 DASPXW010000000
VERSION     DASPXW010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108813"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            340..1764
                     /gene="lpdA"
                     /locus_tag="VEL04_07605"
     CDS             340..1764
                     /gene="lpdA"
                     /locus_tag="VEL04_07605"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HYT48269.1"
                     /translation="MAQTFDVVVIGGGPGGYVAAIRAAQLGLRTACVDDWRGADGKPA
                     LGGACTNVGCIPSKALLQSSENFEQAGHHFADHGVRVKGLELDLAQMLKRKDKVVKAS
                     NDGVVYLFKKNKIAFFHGRGSFIKAGADGCEVAVKGAQNETLAARHVIIATGSNPRAL
                     PGAAFDEKLILSNTGALAIPDVPKRLGVIGAGVIGLEMGSVWRRLGSEVTILEVLPAF
                     LGAADEQIAKEAWKIFTKQGLKIELGVKISGVKAKKDATVEYADAQGKAQSVVFDKLI
                     VSIGRVPNTDGLNAQAVGLRLDERGFIAVDENCRTNLPNVWAIGDVVRGPMLAHKAEE
                     EGAAVAERIAGQRPHVDFNTIPWVIYTAPEIAWVGKTEQQLKAEGLEYRAGTFPFSAN
                     ARARALGDTSGMVKFLADAKTDAVLGVHIMGAFASELIAEAVVAMEFRGAAEDIGIIC
                     HPHPSLSEAMKDAALAVDKRAINI"
     gene            1769..>2364
                     /gene="zapE"
                     /locus_tag="VEL04_07610"
     CDS             1769..>2364
                     /gene="zapE"
                     /locus_tag="VEL04_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HYT48270.1"
                     /translation="MNERALAADERLSVTELYHRAFERRGFTSDPAQLRAVGRLQQLY
                     EEWSAYKARRSSALKRLVVYPPLPRGVYLWGGVGRGKSFLMDSFYLSLPLVRKRRVHF
                     HHFMRDVHRELEELKGREDPLSALAGRIAKRYRLICFDEMHVNDIADAMIVGRLLARI
                     MDLGVVCCMTSNYHPDELYKDGLKRENFLPTIALLKERL"
BASE COUNT          444 a          731 c          810 g          379 t
ORIGIN      
        1 cgggctcgaa gcccgggagc aggaaaaaaa gcgcttcttc gaattcgccg atcgcctctc
       61 gcgctcccgc gacccggagg agcagaaacg tctcaaggaa gaactcgccc gcatgacctt
      121 cggtgagtga tgccgaagat ctgcggcgaa ggacagtatc cgaagacgtt tctgctccga
      181 ggccaacctg ccaaagggga aaaactctag acccagaact gaaaggtgat gcgtccgcgc
      241 tcgagcggct cgatgcgcgg tgtgcgcgcc tttcgaaagc cgaatgagta tggagggcag
      301 tgtcccgtag agtgttttta cgaggggtga agcctgatca tggcccagac atttgacgtc
      361 gtcgtaatcg gcggaggacc gggcggctac gtcgccgcga tccgcgccgc gcagctcggg
      421 ctgaggaccg cctgcgtgga cgattggcgg ggcgcggacg gaaagcccgc gctgggaggc
      481 gcgtgcacca acgtcggctg catcccttcg aaggccttgc tgcagtcctc ggagaacttc
      541 gagcaggccg ggcaccactt cgccgatcac ggcgtgcgcg tgaagggcct cgagctcgat
      601 ctcgcccaga tgctcaagcg caaggacaag gtggtgaagg cgagcaacga cggcgtcgtc
      661 tacctgttca agaagaacaa gatcgcgttc tttcatggcc gcggcagttt catcaaggct
      721 ggcgcggacg gctgcgaggt cgcggtcaag ggcgcgcaga acgaaaccct ggcggccagg
      781 cacgtcatca tcgccaccgg atcgaacccg cgagcgctgc ctggagcggc tttcgacgag
      841 aagctgatcc tgagcaacac cggagcgctc gcgattcccg atgtgccgaa gcgcctcggc
      901 gtgatcggcg ccggcgtgat tggcctggag atgggcagcg tgtggcgccg cctcggctcg
      961 gaggtgacga ttctcgaggt gctgccggcg tttctcggcg ccgccgacga gcagatcgcg
     1021 aaggaggcct ggaagatttt caccaagcag ggactcaaga tcgagctcgg cgtcaagatc
     1081 agcggcgtga aggcgaagaa agacgcgacc gtcgaatacg ctgacgcaca gggcaaggcg
     1141 cagagcgtcg tgttcgacaa acttattgtc tcgatcggac gcgtcccaaa tacggacggc
     1201 ctcaacgcgc aagccgtggg gctcaggctc gacgagcgcg ggtttatcgc cgtcgatgag
     1261 aattgccgca cgaatcttcc caacgtgtgg gcgatcggcg acgtggtgcg cggaccgatg
     1321 cttgcgcaca aggcggagga ggagggcgcg gccgtggccg agcgcatcgc cggccagcgt
     1381 ccgcacgtcg atttcaacac gattccctgg gtgatctaca cggcgcccga gatcgcctgg
     1441 gtcggcaaga ccgagcagca gctgaaggcc gaagggctcg agtaccgcgc cgggacgttt
     1501 ccgttctccg ccaacgcccg ggcgcgcgcg ctcggcgaca cgagcgggat ggtgaagttc
     1561 ctcgccgacg cgaagacgga cgcggtgctc ggcgtgcata tcatgggggc gttcgcctcg
     1621 gagctgatcg ccgaagccgt ggtcgcgatg gagttccgcg gtgcggccga ggacatcggc
     1681 ataatctgcc acccgcaccc ttcgctttcc gaggccatga aggacgccgc actcgccgtg
     1741 gacaagcgcg cgatcaacat ctaggcgaat gaacgagcgc gccctagccg ccgacgagag
     1801 gctctcggtc accgagctct accaccgcgc gttcgagcgg cgcggcttca cgagcgatcc
     1861 cgcgcagctg cgcgcggtcg ggcggctgca gcagctctac gaggaatggt cggcgtacaa
     1921 ggcgcggcgc agctcggcgc tcaagcgcct ggtggtgtac ccgccgctgc cgcgcggcgt
     1981 gtacctgtgg ggcggcgtgg ggcgcggcaa gagcttcttg atggacagct tctacctgtc
     2041 gctgccactg gtgcgcaagc gccgggtgca cttccaccac ttcatgcgcg acgttcaccg
     2101 ggagctcgag gagcttaagg gccgcgagga tcccttgagc gcgctcgccg ggcgcatcgc
     2161 gaagcgctat cgcctgatct gcttcgacga gatgcacgtc aacgacatcg ccgacgcgat
     2221 gatcgtcggg cggctgctcg cgcggatcat ggatctcggc gtggtctgct gcatgacctc
     2281 caattaccat cccgacgagc tctacaagga cggcttgaaa cgcgagaatt tcctgccgac
     2341 catcgcgctg ctcaaggagc gcct
//
LOCUS       DASPXW010000336         3404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_729073, whole genome shotgun sequence.
ACCESSION   DASPXW010000336 DASPXW010000000
VERSION     DASPXW010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729073"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VEL04_07615"
     CDS             <1..309
                     /locus_tag="VEL04_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HYT48271.1"
                     /translation="DRPGVRLSLAAEEAPGEELLQRVKAAFSVDASSTVRYGWLKDLR
                     DLEVPRGRTVCNCLDVAQGEIEAFLAASKSLAELQASLKCGTNCGSCLPELRRMAAAA
                     "
     gene            complement(313..1809)
                     /locus_tag="VEL04_07620"
     CDS             complement(313..1809)
                     /locus_tag="VEL04_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HYT48272.1"
                     /translation="MQEKVITTSFASLGLSAELLRAVSEQGYTETTPIQKQAIPLVLA
                     GRDLLGAAQTGTGKTAGFALPILQRLSGTATASHSPARHPVRALIITPTRELAIQVEE
                     SFRTYGKYSALRSTLVYGGVGIQPQIMELRRGVDILVATPGRLLDHVQQKTVDLRQVE
                     ILVLDEGDRMLDMGFIHDIRKILALLPAKRQNLLFSATFPEEIRKLAASFMKDAATVE
                     VARRNTPAELVAQVAHPVDAMRKRELLTHLVKSNDWKQVLVFCKTKHGANRLAQQLSR
                     EGVHADAIHGNKTQGARVRALEQFKAGKVRVLVATDIAARGLDIEALPHVVNYDLPHV
                     AEDYVHRIGRTGRAGSEGEAVSLFSPEERPLLAAIERLMNRSVEQRVVAGFEPGHHSS
                     RPASQPQHSQGRRPEQHRRPEQHRRPDQNRRPEQRGPRPVQSQPRFARRHEPVRMPAP
                     IAAEQAAQLEEARARMAASGESRPAAPGRRTRRHEPQLGGALLAVKRS"
     gene            2014..2292
                     /locus_tag="VEL04_07625"
     CDS             2014..2292
                     /locus_tag="VEL04_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48273.1"
                     /translation="MQVITINAFVVANMKALEMTGVLMRIFSFSLVSWMGPQSPFMFV
                     WVFNTTDAVLLTWCAVLRKDSAYTVLNGFWILVGLVGMARAGGLLGHF"
     gene            2414..>3404
                     /locus_tag="VEL04_07630"
     CDS             2414..>3404
                     /locus_tag="VEL04_07630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HYT48274.1"
                     /translation="MCKLCDAGFPQNHYGRRDFLKTTAATGAAAAGLNLFSPRPAAAA
                     DGDQPPVDSGKAGRRYIIRGGSVLSMDPKVGDFARADVLVEGKKIQKVGPNLSAGGAD
                     VVDATGRIVMPGFIDTHHHQFETALRSFLANGILINDGSGTLSANPTYYEYILLTFAP
                     VYQPQDVYISELFGGLAQLDDGVTTVHDISQIHHSPAHSDAAIQALFDTGRRAAFGYF
                     ESASGVAGNQYPQDAFRIKKQWFSSSDQLVHMIMGGEVYLGEPTYTQAWQIGRQLGLQ
                     IAAHILSPFGIRPILDDLANGTGGVNNDIGLGPDNLFIHMTGMSDTGWQAVKDK"
BASE COUNT          548 a         1101 c         1163 g          592 t
ORIGIN      
        1 gaccggccgg gggtgcgctt gtcgcttgcg gcagaggagg cgccaggcga agagctcctg
       61 cagcgcgtca aggcagcatt cagcgtcgat gcgtcgagca ccgtgcgcta tggctggctg
      121 aaggaccttc gggacctgga ggtgccgcgc ggccggacgg tatgcaactg cctcgacgtc
      181 gcccagggcg agatcgaggc gttcctggcc gcctcgaaat cgctcgccga gctgcaggcc
      241 agcctcaagt gcgggacgaa ctgcggctcc tgcctgccgg agctgcggcg catggcggcc
      301 gccgcctgag cttcagctcc gcttgactgc gagcagggcg ccgccgagct ggggctcgtg
      361 acggcgtgta cgcctgccgg gcgccgccgg ccgggattct cccgacgccg ccatgcgggc
      421 gcgggcttcc tccagctggg ctgcctgctc agcggcgatc ggcgcgggca tgcgcaccgg
      481 ctcatgccgt cgcgcgaacc tgggctggga ttgaaccggg cgtgggccgc gttgctccgg
      541 gcggcggttc tggtcggggc ggcgatgctg ctcggggcgg cgatgctgct cgggacgtcg
      601 accctgcgaa tgctgcggct gtgacgccgg gcgcgagctg tggtgcccgg gctcgaagcc
      661 cgcaacgacg cgctgctcca ccgaccggtt catcagcctt tcgatcgccg ccagcagcgg
      721 ccgctcctcg gggctgaaga gcgagaccgc ttcaccctcg ctgccggccc ggccggtcct
      781 gccgatgcgg tgcacgtaat cctcggcgac atgcggcagg tcgtagttca ccacgtgcgg
      841 cagcgcctcg atgtcgaggc cgcgcgccgc gatgtcggtt gcgaccagca cccgcacctt
      901 gcctgccttg aactgctcca gcgcgcgcac gcgcgcgccc tgggttttgt tgccgtggat
      961 ggcgtcggca tgcacgccct cgcgggacag ctgctgtgcc agccggtttg cgccgtgctt
     1021 ggtcttgcag aacactagca cctgcttcca gtcgttcgat ttcacgagat gcgtgagcag
     1081 ctcgcgcttg cgcatggcgt cgaccgggtg cgcgacctgc gccaccagct cggccggcgt
     1141 gttgcggcgc gcgacttcca ccgtcgccgc atccttcatg aaggaggccg ccagtttgcg
     1201 gatctcttcg gggaaggtcg cggagaacag caggttctgg cgcttcgccg gcagcagggc
     1261 gaggatcttg cggatgtcgt ggatgaagcc catgtcgagc atgcggtcgc cttcgtcgag
     1321 caccaggatc tcgacctggc gcaagtcgac ggttttctgc tgcacgtggt cgagcagcct
     1381 gcctggtgtc gccaccagga tatcgacgcc gcggcgcagc tccatgatct gcggctggat
     1441 gccgacgccg ccgtaaacta gggtcgatct caatgcgctg tatttcccgt aggtgcggaa
     1501 gctttcctcg acctggatcg ccagctcgcg cgtcggcgtg atgatcagcg cgcgcactgg
     1561 atggcgcgcc ggcgaatggc tggcggtcgc tgttcccgag agacgttgca gaatcggcag
     1621 cgcgaatcca gcggtcttgc cggtgccggt ctgcgccgcg cctaacaggt cgcggcctgc
     1681 gagcacgagg ggaatggcct gcttctggat cggggtggtt tcggtgtagc cctgctcgct
     1741 gacagcgcgg agcagctcgg ccgacaggcc gagtgatgca aacgacgtag taatgacttt
     1801 ctcctgcact cggcctggga aggatcacct tcgaccggtt caggcagatg cgttttaggc
     1861 ttgttattag acggtccagg atggaccgaa caacggactg agacgctaac cggaaatacg
     1921 cgtaacagaa agcttgacgc ccattgtaca cgagaaccgg atcgaccggg cgctgggcat
     1981 aatgcgcgct ttagcgaggg ggaggaggtc aacttgcaag tcataacgat caatgcgttc
     2041 gtcgtcgcga acatgaaggc gctggagatg accggcgtgc tgatgaggat tttcagcttc
     2101 tcgctggtga gctggatggg gccgcagagc ccgttcatgt tcgtgtgggt gttcaacacc
     2161 accgatgccg tgctgctgac ctggtgcgcg gtgctgagaa aggattcggc ctatacggtg
     2221 ctcaatggat tctggatcct cgtcggcctg gtcggcatgg cgagagcggg cggcctgctg
     2281 ggccacttct aagaccgggc gcagtcgctc gcgagaagcg cgttcgcaca tcgcatttac
     2341 acgcggtgcg catcggcgca agaatctgcc tgggtcgtca tcccgcgcga ctcgcataaa
     2401 gggaggaacc accatgtgca agctctgcga tgcaggcttt ccgcagaacc attacgggcg
     2461 ccgcgatttc ctgaagacca cggcggcgac cggggctgcc gcggcgggcc tcaatctgtt
     2521 cagcccgcgc cccgcggcag cggccgacgg cgatcagcct cccgtcgaca gcggcaaggc
     2581 ggggcggcgc tacatcatcc gcggcggctc ggtgttgtcg atggacccga aggtgggcga
     2641 cttcgccagg gccgacgtgc tggtcgaggg caagaagatc cagaaggtcg ggccgaacct
     2701 gagcgcgggc ggcgccgacg tcgtcgacgc caccgggcgc atcgtcatgc ccggcttcat
     2761 cgatacgcac catcaccagt tcgagaccgc gctgcgcagc tttcttgcca acggcatcct
     2821 gatcaacgac ggctcaggca cgctgagcgc gaatccgacg tattacgaat acatcctgct
     2881 caccttcgcg cccgtgtacc agccgcagga cgtctacatc agcgagctgt tcggcgggct
     2941 ggcgcagctc gacgacggcg tcaccacggt gcacgacatc tcgcagatcc atcactcgcc
     3001 cgcgcactcc gacgccgcga tccaggcgct gttcgacacc ggccggcgcg ccgcgttcgg
     3061 ctatttcgag agcgcgagcg gcgtcgccgg caaccagtat ccgcaggacg cgttccgcat
     3121 caagaaacag tggttctcct cgagcgatca gctcgtgcac atgatcatgg gcggcgaggt
     3181 ctacctcggc gagccgacct acacccaggc gtggcagatc gggcgccagc tcggcctgca
     3241 gatcgcggcg cacatcctgt cgccgttcgg catccgcccg atcctggacg atctcgccaa
     3301 cggcacgggc ggcgtcaaca acgacatcgg gctcgggccc gacaatctct tcatccacat
     3361 gaccgggatg tccgacacgg gctggcaggc agtcaaggac aagg
//
LOCUS       DASPXW010000337         3550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_729225, whole genome shotgun sequence.
ACCESSION   DASPXW010000337 DASPXW010000000
VERSION     DASPXW010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729225"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="VEL04_07635"
     CDS             complement(<1..1052)
                     /locus_tag="VEL04_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HYT48275.1"
                     /translation="MGISGRIARFFQDSQLTPLVALVAFLLGLFAVSVTPREEEPQIN
                     VTMANVFVPFPGASARDVEALVATPAEQVLAQMNGVEHVFSVSRPGMAVLTVQFEVGV
                     PRIGALVRLYDTINSNRDWVSPDLGILEPIIKPKGIDDVPVVTLTLWSDDRERGAFEL
                     ERVAHAAEIEFKRVPGTREVQTLGGPGRAVRVLLDPDRLNAYGVSALDVRNALQLANV
                     ALPTGKLVRENREIVVETGNYLESVADVKQLVVGASERRAVFLADVAEVVDGPEQPAR
                     YLWRGFGASAAKKGLRAGAEQPAVTLQITKKPGENAVEVAERVLRRAEELRGTVIPDD
                     VRVTVTRNYGASADEKA"
     gene            complement(1071..2126)
                     /locus_tag="VEL04_07640"
     CDS             complement(1071..2126)
                     /locus_tag="VEL04_07640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HYT48276.1"
                     /translation="MIRNAVLAAVLVLGACGRSEAPREPARAPAVKTDAVEMRDVDLA
                     YSAEAVVEAVRQSTVAAQVSGRIVDLRFDVGDYVKKGEVIVRIDERAAAQAVQASEAQ
                     VRQAEAELRNARAQYERAKQLVAEKFLSPAALDKAESDYRAAQARVAALLAGAEQAAT
                     EKSFTVVAAPYSGVVSARHVELGEMATPGKPLMTGFDPGTLRVTATVPQAQIGAIQAG
                     GKARVEIPSLGKWVDAKRVTIVPSADPRTHTTQVRLELPADVRGVYPGVYARAHFITG
                     TAPRLLVPRAAVLRRSEVTAVYIADDKGALQLRQVRVGTAGDERFIEVLSGLRPGERV
                     ALEPVKAGIESNQRSAN"
     gene            complement(2246..2515)
                     /locus_tag="VEL04_07645"
     CDS             complement(2246..2515)
                     /locus_tag="VEL04_07645"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HYT48277.1"
                     /translation="MHYHYLTIEQRESLEKLIRSQIGAGAQLETALERLHQGDYGVCI
                     ECGKDIGFVRLVADPSTLHCQACARLPVATAKAKSLVSCAIGRLS"
     gene            2875..3309
                     /locus_tag="VEL04_07650"
     CDS             2875..3309
                     /locus_tag="VEL04_07650"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HYT48278.1"
                     /translation="MEVAVFSERIRSVMERKKMLTASPETTVGKAAKLMANKKVGAVM
                     VVEKERLVGIFTERDAVFRVIARGLDAQTTRLADVMTTAPRTVDPDKTFGYALLIMHE
                     NGFRHVPVIENAKPVGIVSARNALDPEMEEFVSEVRRREHIR"
BASE COUNT          595 a         1198 c         1141 g          616 t
ORIGIN      
        1 gccttttcgt cggcgctcgc gccgtagttg cgcgtgaccg tgacgcgcac gtcgtccggg
       61 atcacggtgc cgcgcagctc ctccgcgcgc cgcaggacgc gctcggcgac ctccaccgcg
      121 ttctcgcccg gcttcttcgt gatctggagc gtgaccgccg gctgttccgc gccggcgcgc
      181 aggcccttct tcgcggccga cgcgccgaac cccctccaga ggtagcgcgc cggctgctcg
      241 ggaccgtcga ccacctcggc gacgtcggcg agaaacaccg ctcgccgttc cgacgctccc
      301 accacgagct gcttcacgtc agcgaccgat tccaagtagt tgccggtctc gaccacgatc
      361 tcgcggttct cgcgcaccag cttcccggtg ggcagcgcga cgttggcgag ctgcagcgcg
      421 ttgcgcacgt ccagcgccga aaccccgtag gcgttcagcc ggtccggatc gagcagcacg
      481 cgcaccgcgc gccctgggcc ccccagggtc tgcacttcgc gcgtgcccgg gacgcgcttg
      541 aactcgatct cggcggcgtg cgcgacgcgc tccagctcga atgcgccgcg ttcgcggtca
      601 tcgctccaca gggtgagcgt gaccaccggc acgtcgtcga tgcccttcgg cttgatgatg
      661 ggttcgagga tccccaggtc cggcgaaacc cagtcgcggt tggagttgat ggtgtcgtag
      721 aggcgcacca gggcgccgat gcgcggcacg ccgacctcga actggaccgt gagcactgcc
      781 atgccgggcc gcgacaccga gaacacatgt tcgacaccgt tcatctgcgc gagtacctgc
      841 tccgccggcg tcgcgacgag cgcctcgacg tcgcgcgccg aggcgccggg gaagggcacg
      901 aagacgttcg ccatggtgac gttgatctgc ggctcctcct cgcgcggggt caccgagacg
      961 gcgaacaggc cgagcaggaa cgcgaccagc gcgaccagcg gcgtgagctg cgagtcctgg
     1021 aagaagcgcg cgatccggcc ggagatgccc atgcacgccg tccgctgcgc tcagttcgcg
     1081 gagcgctggt tgctttcgat gcccgccttg acgggctcga gcgcgacgcg ctcgccgggc
     1141 cgcaggcccg acagcacttc gatgaaacgt tcgtccccgg ctgtgccgac tcgcacctgg
     1201 cgcagctgca gcgcgccctt gtcgtccgct atgtatacag cggtcacctc gcttcgccgc
     1261 agcaccgccg cgcgcggcac gagcagcctc ggtgccgtgc cggtgatgaa atgcgcccgc
     1321 gcgtacacgc ccggatacac gccacggaca tcggcgggca gttccagccg cacttgcgtt
     1381 gtgtgcgtgc gcggatcggc cgacgggacg atggtgacgc gcttcgcgtc tacccatttt
     1441 ccgagcgagg gaatctcgac gcgcgccttg ccgcccgcct ggatcgcgcc gatctgcgcc
     1501 tgcgggacgg tggcggtgac gcgcagggtg cccgggtcga aaccggtcat gagcggtttg
     1561 ccgggcgtcg ccatctcgcc cagctcgacg tgccgtgccg agaccacgcc gctgtaggga
     1621 gcagccacaa cggtgaagct cttctcggtc gccgcctgct ccgcgcccgc gagcagcgcg
     1681 gcgacgcgtg cctgcgccgc cctgtagtcc gactcggcct tgtcgagcgc ggccgggctc
     1741 aggaatttct cggcaacgag ctgcttcgcg cgctcgtact gcgcgcgcgc gttgcgcagt
     1801 tcggcttccg cctgccgcac ttgtgcctcg ctcgcctgga ccgcctgcgc ggcggcgcgc
     1861 tcgtcgatgc gcacgatcac ctcgcccttc ttcacgtagt cgccgacgtc gaagcgcaga
     1921 tcgacgatgc gccccgagac ctgcgcggcg accgtggact ggcgcaccgc ctcgaccacg
     1981 gcctcggccg agtacgcgag atcgacgtcg cgcatctcga ctgcgtccgt cttgacggct
     2041 ggggcgcgtg cgggctcgcg cggggcttcg ctccgcccgc aggcgccgag cacaagcacc
     2101 gcggcgagga ccgcgttcct tatcatgtcg tcgccttcgc gctgcgccgt gtcgccaggc
     2161 gaacgagcgt gcccgcgtcc gtcgcgcctg agatccgctc cgcctcgctc ccgttttcga
     2221 actggatgag ccacccgatc gccggtcagg aaagccggcc gatggcgcag ctgacgagcg
     2281 atttcgcctt tgccgtggcg acgggcaatc gggcgcaggc ctggcagtgc agggtcgaag
     2341 ggtcggccac gaggcggaca aaccctatgt ccttgccgca ttcgatacac acgccgtagt
     2401 ctccctgatg gaggcgctca agggcggtct ccagttgcgc cccggcgcca atctgcgagc
     2461 ggatcagctt ctccagactc tcgcgctgtt cgatggtcag gtaatgatag tgcacgctgg
     2521 actccttagc agacgacgag caccaggact ctggaactcg agagcatgcg gggttcgtaa
     2581 ctcatctcga gttgaagcta ctccttctcc cagtgcacgc tttgatctgc atcaaacaag
     2641 cccgcttccc cagccaagca agcgtcagta atttcggaca aacgataatc ccttgctcac
     2701 acggattccg ctcttgatat aggtcaatag agcaaatcaa gagaacagta gcatcgccgg
     2761 atgggcaagg ctacatgcga acagacgaga gatagcgggt tatgtttgac ttttgccaag
     2821 aggtggccgc gaggtcagcc aggtcgagcc tccgcgtaca tctcaatgtg aggcttggag
     2881 gtcgctgtgt tcagtgaacg gatcagaagc gtgatggagc gaaaaaaaat gctcaccgct
     2941 tctccggaga ccaccgtcgg caaagccgcc aagctgatgg caaataagaa ggtcggcgca
     3001 gtcatggtcg tcgaaaagga acgcctggtc gggattttta ccgagcgcga tgccgtattt
     3061 cgcgtcatcg cgcgaggcct tgatgcgcag accacccggc tggccgatgt gatgacgacc
     3121 gcaccgcgga ccgtcgatcc cgacaagaca ttcggatacg cgctcttaat aatgcacgag
     3181 aacggcttcc gtcacgtacc ggtgatcgaa aacgccaaac ctgtcgggat cgtctcagct
     3241 cgcaatgcgc tggatcccga aatggaagag ttcgtatctg aagtacgacg tcgagagcac
     3301 atccgctaac ctgctctagg ctcctggtat cctttgttag gtaagtgctc gaatttcccg
     3361 caatagccgg aaaaagcggc gaaagtcgat ggacccatca ctgggctagc acccctgtaa
     3421 ggtgtcactg ctggccggtc agcaactccg cggtaagaga ccctaccagc gctcgagctt
     3481 gatgtcgtcg taggaggcgg gctgggagat cggctcgatg tggctgagcg tgatcgcctt
     3541 gggcacgcgc
//
LOCUS       DASPXW010000338         3739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_224440, whole genome shotgun sequence.
ACCESSION   DASPXW010000338 DASPXW010000000
VERSION     DASPXW010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224440"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="VEL04_07655"
     CDS             <1..520
                     /locus_tag="VEL04_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48279.1"
                     /translation="VLQHGGDHANRAAPGGKKSEFPGTEDKNTALGRVAIARARLAEL
                     AGPRALPVFVPPWNRFRQDLAGELSSIGLCGLSGYGARRAAGAAPGLRQVNTHIDIVD
                     WHQGRRFIGEQRAFELAVKYFPGDEPVGWLTHHAVHDAAAWEFLARLFTLRGPRWASA
                     EELFSYTRPAHG"
     gene            513..2387
                     /locus_tag="VEL04_07660"
     CDS             513..2387
                     /locus_tag="VEL04_07660"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT48280.1"
                     /translation="MADLLTVRDLKVYLDVDAGTVKAVDGVSFRVPEGGTIALVGESG
                     SGKSVVAQSITGILPRIARVESGEIVFRDPKLPGTAVDISRLDPRSRAMRHIRGGRIS
                     IIFQEPMTALSPLHTIGNQVTEALALHSPGLGKGERRAAVLEMLRLVGFPDPVKAEHT
                     YSFELSGGLRQRAMIAMALVSRPALLIADEPTTALDVTIQAQILQLIRELQGSLGMAV
                     LLITHDLGVVANMADEVVVMHRGRVMEAGTLTDIFRAPRHPYLKALMRAVPRFDMAPG
                     ERLTPIREVQITGGSLLAGAGRTAGTRDLPLLEVKNLTKHYATRKRSLLGRRLGGEVL
                     AVDDLSLEVGTGECLGLVGESGCGKTTTARMILRSVTPDAGSIVFQEKDVLALRGAEL
                     FAYRRKVQFVFQDPFGSLNPRMTAYDIVSEPLVIHRVGDADERFSRVKEMMGLVGLDM
                     RYLRRYPHSFSGGQRQRIGIARALALGPELLICDEPVSALDVSVQAQVLNLLADLRSA
                     LNLTYIFISHNLAVVNYLADRIAVMCAGRIVELAPRGELFRKPSHPYTKALLAAVPVP
                     NLERKLDFDALMQGKASDPAAWPGAFRIAGAARPGMLHLGNGHYVRARDTADATEVAA
                     "
     gene            2444..>3739
                     /locus_tag="VEL04_07665"
     CDS             2444..>3739
                     /locus_tag="VEL04_07665"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYT48281.1"
                     /translation="METPMLRLEVAAGRLPGIEKRLPQQPLVVHLGAGAAYGKHGGTL
                     QTLAGRSRDTRLFTIYGYARLVGYDRDLNIVPDLLESLEVKEGRVFTLRLRKGHRWSD
                     GHPFTSEDFRYYWEDVANNRELSPSGPPRDLLVDGEPPKFELLSETAVRYSWRLPNPN
                     FLPRLAGTSPLFIYRPAHYLRKFHKKYSAKVAKAEAEGTATRRWSAVHNRLDNLYESD
                     NPDLPTLQPWMNTTRPPADRFVAVRNPYFHRVDERGRQLPYLDRFVIAIADAKLIPAK
                     TGAGEADLQARDLHFNNYTFLKQNEKLNGYRTLLWRAAKGSHVALFPNLNVNDPVWRA
                     VLRDARFRRALSLAIDRSLVNQVLYFGLAIESNNTVLAASPLYKESYRDAWARYDRRA
                     AQRLLDEMGLKRGRDGIRKLPDGRPLELIVETAGESSEQT"
BASE COUNT          583 a         1325 c         1248 g          583 t
ORIGIN      
        1 ggtgttgcag cacggcggcg accacgccaa ccgcgccgcg cccggcggca agaagtctga
       61 atttcccggg actgaagata aaaacacggc gctcgggcgg gtcgccattg cgcgcgcgcg
      121 gctcgcggag cttgccggcc cgcgcgcact gccggttttc gtgccgccct ggaacagatt
      181 tcggcaggac ctggcgggtg agctctcttc gatcgggttg tgcggcctga gcggctacgg
      241 tgcgcgccgg gcggccggag ccgcgccggg cctgcgccag gtcaacaccc acatcgacat
      301 cgtcgactgg caccaggggc gccgcttcat cggcgagcaa cgcgcctttg aacttgccgt
      361 gaagtatttt cctggagacg aacccgtggg gtggctcacg catcacgcag tgcacgatgc
      421 cgccgcctgg gaattcctcg cgcgtctttt taccctccgc ggcccgcgct gggcctcggc
      481 cgaagaactg ttctcgtata ctcgcccggc gcatggctga cctgcttacc gtacgcgatc
      541 tcaaggtcta cctcgacgtc gacgccggca ccgtgaaggc ggtcgacggc gtgtcgttcc
      601 gcgtccccga gggcggcacg atcgcgctgg tgggcgaatc gggctcgggc aaatcggtgg
      661 tggcgcagtc gatcacgggc atcctgccgc gcatcgcgcg cgtcgagtcg ggcgagatcg
      721 tgttccgcga tccgaagctt cccggcaccg cggtcgacat ctcgcgcctc gacccgcgca
      781 gccgcgcgat gcggcacatc cgcggcgggc gcatctcgat catcttccag gagccgatga
      841 cggcgctctc gccgctgcat accatcggca accaggtcac cgaggcgctc gcgctgcaca
      901 gccccggcct cggcaagggc gagcgccgcg ccgcggtgct ggaaatgctg cggctggtgg
      961 gctttcccga cccggtcaag gccgagcaca cctactcgtt cgagctctcg ggcggcctgc
     1021 gtcagcgcgc catgatcgcg atggcgctgg tgagccggcc ggcgctgctg atcgccgacg
     1081 agccgaccac tgcgctcgac gtcacgatcc aggcgcagat cctgcagctc atccgcgagc
     1141 tgcagggcag cctcggcatg gccgtgctgc tcatcacgca cgacctcggc gtggtggcga
     1201 acatggccga cgaggtggtg gtgatgcacc gcggccgcgt catggaggcc ggcacgctca
     1261 ccgacatctt ccgcgcgccg cgccacccct atctcaaggc actgatgcgc gcagtgccgc
     1321 gcttcgacat ggcgccgggc gagcgcctga cgccgatccg cgaggtgcag atcaccggcg
     1381 gcagcctgct cgccggggcc gggcgcacgg cgggtacgcg ggacttgccg ctactggaag
     1441 taaagaatct tacgaaacat tatgcgaccc gcaaacgctc cctcctcgga cgcaggctcg
     1501 gcggcgaagt gctcgcggtc gacgacctga gcctggaagt cggcaccggc gaatgcctgg
     1561 ggctggtggg cgagtcgggc tgcggcaaga ccaccaccgc gcgcatgatc ctgcgctcgg
     1621 tgacgcccga cgcgggaagc attgtttttc aggaaaaaga tgtcctcgct ctcaggggcg
     1681 cggagctgtt cgcttaccgg cgcaaagtgc agttcgtgtt ccaggatccc ttcggctcgc
     1741 tcaacccgcg catgaccgcg tacgacatcg tcagcgagcc gctcgtcatt catcgcgtcg
     1801 gcgacgccga cgagcgcttc tcgcgggtga aggagatgat gggactggtc ggcctggaca
     1861 tgcgctacct gcgccgctac ccccacagct tctccggtgg ccagcgccag cgcatcggca
     1921 tcgcgcgcgc gctcgcgctc gggcccgagc tgctgatctg cgacgagccg gtctcggcgc
     1981 tcgatgtctc ggtgcaggcg caggtgctga acctgctcgc cgacctgcgc tcggcgctca
     2041 acctcaccta catcttcatc tcgcacaacc ttgcggtggt caactacctc gccgaccgca
     2101 tcgcggtgat gtgcgccggg cgcattgtcg agctggcgcc gcgcggtgag ctgttccgca
     2161 agccctcgca tccctacacc aaggcgctgc tcgccgcagt gccggtgccg aacctcgagc
     2221 gcaagctcga cttcgatgcg ctgatgcagg gcaaggcctc ggatcccgcc gcctggccgg
     2281 gggcgttccg tatcgcgggc gcggcgcgcc cgggcatgct gcatctgggc aacggccact
     2341 acgtgcgcgc gcgcgacacc gccgacgcga cagaggtggc ggcgtgaggg gtttcttttc
     2401 cttgctgctg atgcttccag gcatttccct gggagcagat gcgatggaaa ccccgatgct
     2461 tcgcctcgaa gtcgcggcag gaaggctgcc cggcatcgag aagcggctgc cgcagcagcc
     2521 gctggtggtg cacctcggcg cgggggccgc gtacggcaag cacggcggca cgctgcagac
     2581 gctcgccggc cgcagccgcg acacgcgcct gttcaccatc tacggctacg cgcgcctggt
     2641 cggctacgac cgcgatctca acatcgtgcc cgacctgctc gagtcgctcg aggtgaagga
     2701 ggggcgggtt tttaccctca ggctgcgcaa ggggcatcgc tggtccgacg gccatccctt
     2761 cacctcggaa gacttccgct attactggga ggacgtggcg aacaaccgcg agctctcgcc
     2821 ctccgggccg ccgcgcgacc tgctggtcga cggcgagccg ccgaagttcg agctgctgag
     2881 cgagaccgcg gtgcgctaca gctggcgcct gccgaacccg aatttcctgc cgcggctggc
     2941 gggcacctcg ccgctcttca tctaccgccc ggcgcactac ctgaggaagt tccacaagaa
     3001 gtactcggcg aaggtggcca aggcggaggc cgaaggcacg gcgacgcggc gctggtcggc
     3061 ggtgcacaac cgcctcgaca acctgtacga gtcggataac cccgacctgc cgacgctgca
     3121 gccgtggatg aacaccacgc gcccgcccgc ggaccgcttc gtcgccgtgc gcaaccccta
     3181 tttccaccgc gtcgacgagc gcggccggca gctgccgtac ctcgaccgct tcgtcatcgc
     3241 gatcgcggac gccaagctga tcccggccaa gaccggcgcc ggcgaggccg acctgcaggc
     3301 gcgcgacctg catttcaaca actacacctt cctcaagcag aacgagaagc tgaacggcta
     3361 ccgcacgctg ctgtggcgcg ccgcgaaagg ctcgcacgtc gcgctgttcc cgaacctcaa
     3421 cgtcaacgac ccggtgtggc gcgcggtgct gcgcgacgcg cgcttccggc gcgcgctgtc
     3481 gcttgcgatc gaccgctcgc tggtcaacca ggtgctctac ttcggcctcg cgatcgagtc
     3541 caacaacacc gtgctcgccg cgagccccct ctacaaggag agctaccgcg atgcctgggc
     3601 gcgctacgac agaagggccg cgcagcggct gctcgacgag atggggctga agcgcggccg
     3661 cgacggcatc cgcaagctgc ccgacggccg gccgctcgag ctgatcgtcg agaccgcggg
     3721 cgagagctcc gagcagacc
//
LOCUS       DASPXW010000339         2457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1486151, whole genome shotgun sequence.
ACCESSION   DASPXW010000339 DASPXW010000000
VERSION     DASPXW010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1486151"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /gene="rpoN"
                     /locus_tag="VEL04_07670"
     CDS             <1..960
                     /gene="rpoN"
                     /locus_tag="VEL04_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014618027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HYT48282.1"
                     /translation="QSLEEIAALLPAEAEAAPEELSIALRHLQNLEPAGVGARSPGEC
                     LCLQLKTAEKDAVRDLALEIAEKHLELLAGRDYTRLKNLTGAGDDALRAAQRLILALN
                     PRPGAAFARVETRYVIPDVVVRKSRNVWRATLNAEAMPRLRINRLYAELAAGARSAAS
                     GLSSQLQEARWLIKNVQQRFDTILRVSQAIVDRQRHFLEHGEVAMRPLVLREIAQTLG
                     LHESTISRVTTQKYMATPRGTFELKYFFGSHVATEAGGAASSTAIRALIKQLVAAEDA
                     RAPLSDARISQILGEQGIVVARRTIAKYRESLQIPPVNQRKAL"
     gene            963..1256
                     /gene="raiA"
                     /locus_tag="VEL04_07675"
     CDS             963..1256
                     /gene="raiA"
                     /locus_tag="VEL04_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HYT48283.1"
                     /translation="MNLNVSGHHLEVTPAIRSYVKSKLERVTRHFDHVIDAHVILTVD
                     KLRQKAEVTLHVSGKELHCESEEDDLYAAIDLLADKLDRQVLRYKDKLHEKRI"
     gene            1272..1757
                     /gene="ptsN"
                     /locus_tag="VEL04_07680"
     CDS             1272..1757
                     /gene="ptsN"
                     /locus_tag="VEL04_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004534308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HYT48284.1"
                     /translation="MSLVAKLLSPSHVVLDLQASSKKRLFEQAGLLFENRDGIARSLV
                     FDSLLARERLGSTGLGQGVAIPHGRIKGLKEALGAFVRLAQPVPFDAPDGNPVSLVFV
                     LLVPEKATEKHLQLLSELAQMFCDKALREAMSRAARADELHQLITAWQPDAAGQRRAA
                     A"
     gene            1726..>2457
                     /locus_tag="VEL04_07685"
     CDS             1726..>2457
                     /locus_tag="VEL04_07685"
                     /inference="COORDINATES: protein motif:HMM:NF019118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HYT48285.1"
                     /translation="MLQVNVARLHEDNREALSLAWVAGREGGTAVRREAAAAAELIGH
                     LNLTHPNSIQVIGAYEAGIVAGFVERLFESAPAAVIVADGVAPPQPLIDAATRSRTPL
                     FTSPLPAPRVIEKLARYLAKALAETVERHGVLMDVLGLGVLITGESGVGKSELGVELI
                     SRGHGLVADDVVQLSRIAANALEGRCPPMLKDFIEVRGLGLLNIRTIFGETAVRRKMK
                     LRLIAHLEKPVPGGGDPSERLPLAEL"
BASE COUNT          417 a          849 c          828 g          363 t
ORIGIN      
        1 cagtcgctcg aggaaatcgc ggcactgctg ccagccgagg ccgaagccgc tcccgaggag
       61 ctgtcgatcg cgctcaggca cctgcagaac ctggagcccg cgggcgtcgg tgcgcgctcg
      121 cccggcgaat gtttgtgctt acaactaaaa accgcggaaa aagatgccgt gcgcgacctc
      181 gcgctcgaga tcgccgaaaa gcacctggag ctgctcgccg ggcgcgacta cacgcgcctg
      241 aaaaacctca ccggtgcggg cgacgatgcg ctgcgcgccg cgcagcggct gatcctcgcg
      301 ctcaatccgc gcccgggagc ggcattcgcc agggtcgaga cgcgctacgt gattcccgac
      361 gtggtcgtca ggaagagccg caacgtgtgg cgcgcgacgc tcaatgcgga ggcgatgccg
      421 cgcctgcgca tcaaccggct ctacgccgag ctcgccgcgg gcgcgcgcag tgccgcgagc
      481 ggcctctcct cgcagctcca ggaggcgaga tggctgatca agaacgtgca gcagcgcttc
      541 gacacgatcc tgcgggtgtc gcaggcgatc gtcgaccgcc agcgccattt cctcgagcac
      601 ggcgaagtgg cgatgcgccc gctggtgctg cgcgaaatcg cgcagacgct gggcctccac
      661 gagagcacca tctcgcgcgt cacgacgcag aaatacatgg cgacgccgcg cggcaccttc
      721 gagctgaaat acttcttcgg cagccatgtc gccaccgagg cgggcggcgc ggcgtcttcg
      781 accgcgatcc gggcgctgat caagcagctg gtcgccgccg aggacgcgcg cgcgccgctc
      841 tcggacgcgc gcatctcgca gatcctcggc gagcagggta tcgtggtggc gcggcgcacc
      901 atcgcgaagt accgggagtc gctgcagatc cctcccgtca accagagaaa ggccttgtga
      961 ccatgaacct gaacgtgagc ggccatcatc tcgaagtgac ccccgcgatt cgcagctacg
     1021 tgaagtcgaa gctggagcgt gtgacccggc atttcgacca cgtgatcgac gcccacgtca
     1081 tcctcaccgt ggacaagctc aggcagaagg ccgaagtcac gctgcacgtg agcggcaagg
     1141 agctgcattg cgagagcgag gaggacgatc tttacgcggc gatcgatctg cttgccgaca
     1201 agctggaccg ccaggtgctg cgctacaagg ataagctcca cgaaaaacgc atataattcg
     1261 cgccctcttc gatgagcctg gtcgctaagc tactctcccc ttcccacgtc gtgctcgacc
     1321 tccaggcctc gagcaagaag cgcttgttcg agcaggccgg gctgctgttc gagaaccggg
     1381 acggcatcgc gaggagcctg gtgttcgaca gcctgctcgc gcgcgagcgg ctcggctcga
     1441 ccggcctcgg gcagggcgtc gccattccgc acgggcgcat caaggggctc aaggaggcgc
     1501 tcggcgcatt cgtgcggctg gcgcagccgg tgcccttcga cgcgcccgac ggcaaccccg
     1561 tcagcctggt tttcgtcctg ctggtgccgg agaaggcgac cgagaagcac ctgcagctcc
     1621 tctccgagct ggcgcagatg ttctgcgaca aggcgctgcg cgaggcgatg agccgcgccg
     1681 cccgcgccga cgagcttcac cagctgatca cggcatggca gcccgatgct gcaggtcaac
     1741 gtcgcgcggc tgcatgagga caaccgcgag gcgctctcgc tcgcctgggt ggccgggcgc
     1801 gaaggcggca ccgcggtgcg tcgcgaggcg gctgcggccg ccgagctgat cggccacctc
     1861 aacctcaccc accccaacag catccaggtc atcggcgcgt acgaagccgg catcgtcgcc
     1921 gggttcgtcg agcggctgtt cgagtcggcg ccggccgcgg tgatcgtggc cgacggcgtc
     1981 gcgccgccgc agcctctcat cgacgcggcc acgcgctcgc gcacgccgct tttcacctcg
     2041 ccgctgccgg cgccgcgcgt gatcgagaag ctcgcgcgct acctcgccaa ggcgctcgcc
     2101 gagacggtcg agcgccacgg cgtgctgatg gacgtgctcg gcctgggcgt gctgatcacc
     2161 ggcgaatccg gcgtcggcaa gagcgagctc ggggtcgagc tcatcagccg cgggcacggc
     2221 ctggtggccg acgacgtggt ccagctctcg cgcatcgccg cgaatgcgct cgaaggacgc
     2281 tgcccgccga tgctcaagga cttcatcgag gtgcgcggcc tggggctgct gaacatccgc
     2341 accatcttcg gcgagaccgc agtgcgccgc aagatgaaac tcaggctgat cgcgcatctg
     2401 gagaagcctg tgcctggcgg gggcgacccg tccgagcgcc tgccgctcgc cgaattg
//
LOCUS       DASPXW010000340         4652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_475880, whole genome shotgun sequence.
ACCESSION   DASPXW010000340 DASPXW010000000
VERSION     DASPXW010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4652
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475880"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..109)
                     /locus_tag="VEL04_07690"
     CDS             complement(<1..109)
                     /locus_tag="VEL04_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HYT48286.1"
                     /translation="MAGKSRAGLTHFDAGGSARMVDVGAKPRTRRVAVAS"
     gene            131..1570
                     /locus_tag="VEL04_07695"
     CDS             131..1570
                     /locus_tag="VEL04_07695"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HYT48287.1"
                     /translation="MRSTAWLVATFIALAPRAFGQALPDLGSAGDAALSPALERRLGE
                     SIMRDVRFREPAYVDDAEVSEYLANLGGRLAQGSGARQDFEFFAIRDPTINAFALPGG
                     FIGVHTGLILAADTESELASVLGHEMGHVTQRHIARMLGQQQQMQMPMMVALAAAILL
                     GRSRPDLAAGAIAGSQAGAVQTQLGYSRDFEREADRIGLQSLDAAGFDARAMAVFFEK
                     MQRAARVADDGTMPGYLRTHPVTFERIADAQNKAANLPYRQHLDSAEFQLVRAKLRAE
                     AGDARDAVSHFDASVREHRYASEMAARYGLASALLRARRAREADAEMNRLRASGAVGP
                     MIETLAARIKQALGEPQRAAALLADARTRYPHSRPLLYAHVAALQESARNQEALAELA
                     EPMRLYPRDAALHALRAKCYAALGKRLLQHQAQAEVYALQGSLPAAIEQLQLARSAGD
                     GDFYQLSVVDARLKELRSQHAAEARDAKR"
     gene            complement(1571..2530)
                     /locus_tag="VEL04_07700"
     CDS             complement(1571..2530)
                     /locus_tag="VEL04_07700"
                     /inference="COORDINATES: protein motif:HMM:NF021357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HYT48288.1"
                     /translation="MDKSVPEAGAGWLVTLAQLPTEDPAARMRVLRTLESLGSAVIRE
                     GAYLLPDTPDNRRALDALAEYIAKSAGSTHVLHVATASPAQQAGFQRLFDRSARYEEL
                     IKTVESLRVGFGQTDPSGISRVLHKQRREFESIAALDFFPTEARSRAQNALTDSEAEV
                     KKLLFPAQAHAALGPGEALLRRTWATRKPLWADRLACAWLIRRFVDPEATMAWLEKTD
                     ALPAGAVGFAFEGAHFANSDLRVTYEEMLARLELAKNPALTKIGSIVHFLEVRGTPVA
                     EAAGVQTLLQGALRRSANETELLGEVEKTFDLLYEAYFEPPKR"
     gene            2546..2773
                     /locus_tag="VEL04_07705"
     CDS             2546..2773
                     /locus_tag="VEL04_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003263554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HYT48289.1"
                     /translation="MHFDKELDARGLNCPLPILRAKKALTDLQSGQVLKVLATDPGAV
                     KDFQAFSRQTGHELMSHAEANREFTFFMRKK"
     gene            2770..3258
                     /locus_tag="VEL04_07710"
     CDS             2770..3258
                     /locus_tag="VEL04_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HYT48290.1"
                     /translation="MRRVYAALALLAGFSCNAADATHSVRLLTPETALKAAQAALARC
                     RASGYQVTVAVVDRMGVVQVLLRDRFAGPHSVDLASAKAYTAVSFRTNTSALAEATQP
                     GRPQSGIRNRPGVAAVGGGLVIEAGGSLLGAIGVSGAPGGREDDACAEAGIAAIREDI
                     EL"
     gene            3272..3757
                     /locus_tag="VEL04_07715"
     CDS             3272..3757
                     /locus_tag="VEL04_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48291.1"
                     /translation="MMARWIRLGEREAAEFHALCARLAAVQAGRAPPVLAWADAGTQY
                     LFALIAPRKFAPGRSARWLAWGLAPAVATYRQFGLPAYLEGKGIWLHGQRIAEARVQA
                     IGECAVIASSFLARFPAKCTATPSSAFELAFRLRLEAQHGWQFEHSWPTAPERLEHAL
                     A"
     gene            3797..4348
                     /locus_tag="VEL04_07720"
     CDS             3797..4348
                     /locus_tag="VEL04_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48292.1"
                     /translation="MLLVRDALAQGRVEKGVRRRSGDVRISGETISTGADGQAVFVVG
                     RDAMMVRPNSSLSLVSNGFRLVTGAVLSVFAPGEARQLRTPTATIGIRGTAVYLEAEA
                     SRTYVCTCYGEAVLEPLAEPAARETVRTRHHEQPRYIMPKGAPQMIVRAPMINHSDAE
                     LEMLEALVGRKAPFSGGPPYNPP"
     gene            complement(4345..>4652)
                     /locus_tag="VEL04_07725"
     CDS             complement(4345..>4652)
                     /locus_tag="VEL04_07725"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYT48293.1"
                     /translation="ALPALYLVGLGLMLSAASTNTVLQTIVPDELRGRVAALYVMAFI
                     GTAPLGALAAGWIAERVGPPLTLAACGIAGLAAVLVYASRFEAIRREIRPLYDKIGI"
BASE COUNT          704 a         1623 c         1641 g          684 t
ORIGIN      
        1 cggaggcgac cgcgacacgg cgcgtcctcg gcttggcgcc cacgtcgacc atgcgggccg
       61 aacctccggc gtcgaaatga gtcaaaccag cgcgcgattt ccccgccatt ccgggcgcta
      121 tcataactag atgcgcagca ccgcgtggct tgtggccaca ttcatcgctc tggcaccgcg
      181 cgccttcggc caggccttgc ccgatctcgg cagcgccggc gatgcggcgc tgtcgcccgc
      241 gctcgagcgc cggctcggcg agagcatcat gcgcgacgtc cgcttccggg agccggccta
      301 cgtggacgat gccgaggtct ccgaatacct cgcgaacctc ggtggccgcc tcgcgcaggg
      361 cagcggcgcg cgccaggact tcgagttctt cgccatccgc gacccgacca tcaacgcgtt
      421 cgcgcttccc ggcgggttca tcggcgtgca cacgggtctg atcctggcag cggacaccga
      481 gtccgagctc gcctcggtgc tgggacacga gatggggcac gtcacgcagc ggcacatcgc
      541 ccgcatgctc ggccagcagc agcagatgca gatgccgatg atggtcgcgc tcgccgccgc
      601 gatccttctg ggccgttcgc gtcccgacct cgccgcgggc gccatcgcgg ggagccaggc
      661 gggcgcggtg cagacgcagc tcggctactc gcgcgatttc gaacgcgagg cggaccgcat
      721 cggcctgcag tcgctcgacg cggcggggtt cgatgcgcgc gcgatggcgg tattcttcga
      781 gaagatgcag cgcgcggcgc gcgtcgcaga cgacggcacc atgccgggct acctgagaac
      841 ccacccggta accttcgagc gcatcgcgga tgcgcagaac aaggccgcca acctgccgta
      901 ccggcagcat ctcgatagcg ccgaattcca gctcgtccga gccaagctgc gcgccgaggc
      961 gggcgatgcg cgcgacgcgg tgagccattt cgatgcctcg gtgcgcgagc accgttacgc
     1021 cagcgagatg gcggcgcgct acgggctcgc cagcgcgctg ttgcgggcga ggcgggcgcg
     1081 cgaggccgac gcggaaatga accggctgcg cgcgagcggg gccgtcggac ccatgatcga
     1141 gacgctggcc gcgcgcatca agcaggcgct gggagagccg cagcgtgctg cggcgctgct
     1201 cgccgacgcc cgcacacgct atccgcattc gcgtcccttg ctgtacgccc acgtcgccgc
     1261 cctgcaggag tctgcccgca accaggaagc gcttgcggag ctcgccgagc cgatgcgcct
     1321 ttatccgcgc gacgcggcgc tgcatgccct gcgggcgaag tgctacgcgg cgcttggcaa
     1381 gaggctgctg cagcaccagg cgcaggccga ggtgtatgcc ctgcagggca gcctgccggc
     1441 ggcgatcgag cagctgcagc tcgctcgcag cgccggcgac ggcgacttct accagctctc
     1501 ggtggtcgat gcgcgcctca aggagctgcg ttcccagcac gcggccgagg cgcgcgacgc
     1561 gaagcgctga tcagcgcttc ggcggctcga aatacgcctc gtacagcagg tcgaaggtct
     1621 tctcgacttc gccgagcagc tcggtttcgt tcgcagaacg gcgcagcgcg ccctgcagca
     1681 gcgtctgcac gcccgccgcc tcggccaccg gcgtgccgcg cacctccaga aaatgcacga
     1741 tgctgccgat cttcgtcagc gcggggttct tcgcaagctc gagccgcgcc agcatttcct
     1801 cgtaggtgac gcggaggtcg ctgttggcga agtgcgcgcc ctcgaaggca aaaccgacgg
     1861 cgccggcagg cagggcgtcg gtcttctcga gccaggccat ggtcgcctcc gggtcgacga
     1921 agcgccggat cagccaggcg caggccaggc gatcggccca caagggtttg cgggtggccc
     1981 aggtgcgccg cagcagggcc tcgccggggc cgagcgcggc gtgcgcctgc gccgggaaca
     2041 gcagcttttt cacctcggcc tcggagtcgg tgagcgcgtt ctgcgcgcgg ctgcgcgcct
     2101 cggtcgggaa aaagtccagc gccgcaatgc tttcgaactc gcgccgctgc ttgtgcagca
     2161 cccgcgagat gccgctcggg tcggtctggc cgaagccgac gcgcaggctc tcgacggtct
     2221 tgatcagctc ctcgtagcgc gccgagcggt cgaacaggcg ctggaatccc gcctgctgcg
     2281 cgggcgacgc tgtcgcgacg tgcaatacgt gcgtgctgcc ggcgcttttc gcgatgtatt
     2341 cggcgagcgc atcgagggcg cgccggttgt ccggggtgtc cggcagcagg taggcgcctt
     2401 cgcggatcac cgcggagccg agcgactcga gcgtgcgcag cacgcgcatg cgcgccgccg
     2461 gatcctcggt cggcagctgc gccagcgtca ccagccagcc ggcgccggcc tccgggacgg
     2521 acttgtccat gcggcataat actttatgca tttcgacaaa gagctcgatg cccgcgggct
     2581 gaactgcccg ctgccgatcc tgcgcgccaa gaaggccctc accgacttgc agtcgggaca
     2641 ggtcctgaag gtgctcgcca ccgatccggg cgcggtgaag gacttccagg ccttctccag
     2701 gcagaccggg catgagctga tgtcgcatgc cgaagcgaac agggaattca ccttcttcat
     2761 gaggaagaag tgaggcgggt gtacgcggcg cttgctctcc tcgccggatt ttcgtgcaat
     2821 gcagccgacg ccacccacag cgtcaggctg ctcacccctg aaaccgcgct caaggcggcg
     2881 caggccgcgc tcgccaggtg ccgcgcgagc ggctaccagg tgacggtcgc ggtcgtcgac
     2941 cgcatgggcg tggtccaggt gctgctgcgc gaccgctttg ccgggccgca cagcgtcgac
     3001 ctggcgagcg ccaaggctta tacggcggtg agcttccgca ccaataccag cgcgctggcg
     3061 gaagcgacgc agcccggccg gccgcaaagc ggcatccgca accgtcccgg cgtggcggcg
     3121 gtgggcggcg gcctggtgat cgaggccggc ggttcgttgc tcggcgccat cggcgtttcc
     3181 ggcgcgcccg gcggccgcga ggacgacgcc tgcgcggagg cgggtatcgc cgcaatccgc
     3241 gaagacatcg agctgtgaaa taatccggcg catgatggcg cgctggatcc ggctcggcga
     3301 gcgcgaagcc gccgagtttc acgctctctg tgcgcggctc gccgcggtgc aggcggggcg
     3361 cgcgccgccg gtgctcgcct gggcggacgc cggcacccag tacctgttcg cgctcatcgc
     3421 gccgcgcaag ttcgctcccg gccgcagcgc gcgctggctc gcctgggggc tcgcgccggc
     3481 ggtcgccacg tatcgccagt tcggcctgcc ggcgtatctc gaaggcaagg gcatctggct
     3541 gcacgggcag cgcatcgccg aagccagggt gcaggccatc ggcgagtgcg cggtgatcgc
     3601 gtcgagcttt ctcgcgcgct ttccggccaa gtgcaccgcg acgccgtcgt cggcattcga
     3661 gctggcgttc cggctgcggc tcgaggcgca gcatggctgg cagttcgagc attcctggcc
     3721 gaccgcgccc gagaggctcg agcacgcgct cgcctagatg ccaacgcgcc gcgacttgct
     3781 gctaggcacc gcagccatgc ttctggtgcg cgacgcgctc gcgcaggggc gcgtggaaaa
     3841 gggagttcgc cggcgcagcg gcgacgtgcg gatcagcggc gaaacgattt ccaccggcgc
     3901 ggacgggcag gcggtgttcg tcgtcggcag ggacgccatg atggtgcggc ccaattcatc
     3961 gctgagtctg gtgagcaacg gctttcgcct ggtgaccggc gccgtgctct cggtattcgc
     4021 cccgggcgaa gccaggcagc tgcgcactcc gactgccacc atcggcatcc gcggcaccgc
     4081 cgtttacctc gaggccgaag cgagccgcac ctatgtctgc acctgctacg gcgaagcggt
     4141 gctcgagccg cttgcggagc ccgcggcgcg cgaaaccgtg cgcacccggc atcacgagca
     4201 gccgcgctac atcatgccga agggcgcgcc gcagatgatc gtgcgggcgc cgatgatcaa
     4261 tcacagcgac gcggagctcg agatgctgga agccctggtc gggcgcaaag cgccgttctc
     4321 gggcgggcct ccctacaacc ctccctagat tccgatcttg tcgtaaagcg gccggatctc
     4381 gcggcggatc gcctcgaacc ggctcgcata caccagcacc gctgcgagcc cggcgatgcc
     4441 gcacgcagcg agcgtgagcg gcggaccgac acgttcggcg atccagccgg cggcgagcgc
     4501 gccgagcggc gcggtgccga tgaacgccat gacgtagagc gcggcgacac ggccgcgcag
     4561 ctcgtcgggc acaatcgttt gcaggaccgt gttggtcgat gcggcggaca gcatcaatcc
     4621 caggccgacg aggtagaggg caggcagcgc ca
//
LOCUS       DASPXW010000341         2107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1611197, whole genome shotgun sequence.
ACCESSION   DASPXW010000341 DASPXW010000000
VERSION     DASPXW010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1611197"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1496)
                     /locus_tag="VEL04_07730"
     CDS             complement(<1..1496)
                     /locus_tag="VEL04_07730"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYT48294.1"
                     /translation="MAKLRKAAADFTAAIQQAAICHQRGQLADAERLYTRVLAAAPDH
                     FDALHLLGVLRQQQGRSVEALRLIAAALKRNADSVDALSNHGVVLDALTRHEEAVASY
                     DRALALMPGRADVLGNRGNALLALGRDAEALASYEQALALRPAFAQALSNRGRALANL
                     GRNAEAVESFDRALALKPDDVAALALRGNALFQLERHAEALASYDAALAIEPNAARVL
                     NNRGYALLALARLDEALASYQAALALDPDYAEALVNCGNVLGALDCYEDADRHYDQAL
                     VIEPALAEAKWNKSLLHLSRGRFADGWPLYEQRWLGGIKGAVPRGYPQPRWNGEHMRG
                     TLLVWGEQGLGDQILHAGMIPELAGRAGAVVLEVEPRLVDLFARSFPGVSIVPLAPDL
                     FAGPLDAHVPIGSLGAYLRPDWQAFRPRAYLVADRSRADALRARLKHDGHTVVGLSWR
                     SVAPLIGKSKSAQLTDFAEVLRMPGCRFVDLQYGDTRADRERLERELGV"
     gene            complement(1557..>2107)
                     /locus_tag="VEL04_07735"
     CDS             complement(1557..>2107)
                     /locus_tag="VEL04_07735"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYT48295.1"
                     /translation="WYAERAAELGVRFDEPIPQQIHALCEQPRYPGAPGHARLVGAAD
                     ASLFADWIVAFQREAVPHDPVPSRERLEKAAADGRYSIWIVDGEPVSMAGIVRRTRNA
                     AAIAGVYTPPALRGRGYAGSVTAAVVERAFAEGKTTACLYTDLRNPFSNRCYAKVGFR
                     PACESWYIARRRDPEAAPEAAR"
BASE COUNT          323 a          725 c          776 g          283 t
ORIGIN      
        1 acgccaagct cgcgctccaa gcgttcgcga tcggcgcggg tgtcgccgta ttgcaggtcg
       61 acgaagcggc agcccggcat gcgcagaact tcggcgaaat ccgtgagctg ggcgctcttg
      121 gattttccga tcagcggagc gacgctgcgc caggacaggc cgacgaccgt gtggccgtcg
      181 tgcttgaggc gcgcgcgcaa ggcgtcggcg cggctgcggt cggcgacgag gtacgcgcgc
      241 gggcggaacg cctgccagtc cggccgcaga taagcgccga ggctgccgat cggcacatgc
      301 gcatcgagcg gaccggcgaa cagatcgggc gcgagcggca caatggagac gccggggaac
      361 gaacgcgcga acaggtcgac gaggcgcggc tcgacctcca gcaccacggc gccggcgcgc
      421 ccggcaagct ccgggatcat gccggcgtgg aggatctgat cgcccaggcc ctgctcgccc
      481 cagaccagca gcgtgccgcg catgtgctcg ccgttccagc gcggctgcgg atagccgcgc
      541 ggcaccgcgc ccttgatccc gcccagccag cgctgctcgt agagcggcca gccgtcggca
      601 aagcgtccgc gcgaaagatg gagcagactc ttgttccact tcgcctcggc gagagcgggc
      661 tcgatcacga gcgcctggtc gtagtgccga tccgcgtctt cgtagcagtc gagcgcgccc
      721 agcacgttgc cgcaattgac cagcgcctcc gcataatcgg gatcgagcgc aagcgccgcc
      781 tgatagcttg ccagcgcctc gtcgaggcgc gcgagcgcca gcagcgcgta gccgcgattg
      841 ttcagtacac gggcggcgtt cggctcgata gcgagcgcgg catcgtagct cgccagcgcc
      901 tcggcgtgcc gctcgagctg gaacagcgca ttgccgcgca gtgcgagcgc ggcgacgtcg
      961 tccggcttga gcgcgagcgc gcggtcgaag ctctcgaccg cctcggcgtt ccggccgaga
     1021 ttggcaagcg cgcggccgcg gttggacagc gcctgtgcga acgcgggccg cagtgcaagc
     1081 gcctgctcgt agctggcgag cgcttcggca tcgcgcccaa gcgcgagcag cgcattgccg
     1141 cggttgccga gcacgtcggc gcggccgggc atgagcgcca gcgcgcggtc gtagctcgcg
     1201 accgcctcct cgtggcgcgt gagcgcgtcg agcacgacgc cgtggttcga cagcgcatcg
     1261 accgagtcgg cgttgcgctt gagcgcggcc gcgatcagcc gcagcgcctc gaccgagcgg
     1321 ccctgctgct ggcgcagcac gccgagcaga tgcagggcgt cgaaatgatc gggcgcggcg
     1381 gcgaggacgc gcgtgtagag ccgctccgca tcggcgagct ggccgcgctg gtggcagatc
     1441 gccgcctgct ggatcgccgc ggtaaagtca gccgcggcct tgcggagttt cgccatcgca
     1501 gccctgattc gtgcccgctg cgagtatgac gcgcgcgcgg caaaatcaaa ggctgctcaa
     1561 cgcgcggcct cgggcgcagc ctccggatcg cgacggcgcg cgatgtacca ggactcgcac
     1621 gcgggccgaa agccgacctt ggcgtagcag cggttcgaaa agggattgcg cagatcggtg
     1681 tagaggcagg cggtggtctt gccctccgcg aaggcgcgct cgaccaccgc ggcggtgact
     1741 gacccggcat agccgcgacc gcgcagcgcc ggcggcgtgt agacgcccgc gatggcggcg
     1801 gcgttgcggg tgcggcgcac gatgccggcc atcgagaccg gctcgccgtc gacgatccag
     1861 attgagtaac ggccgtcagc cgcggccttt tcgagccgct cgcggctcgg gacagggtcg
     1921 tgcgggaccg cctcgcgctg gaacgcgacg atccagtcgg caaacaacga cgcgtcggcg
     1981 gcgcccacga ggcgcgcgtg gccgggcgcg ccgggatagc gcggctgctc gcacaacgca
     2041 tggatttgct gcgggatcgg ctcgtcgaac ctcacgccca gttcggcggc gcgctcggca
     2101 taccacg
//
LOCUS       DASPXW010000342         3576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1738526, whole genome shotgun sequence.
ACCESSION   DASPXW010000342 DASPXW010000000
VERSION     DASPXW010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1738526"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..628)
                     /locus_tag="VEL04_07740"
     CDS             complement(89..628)
                     /locus_tag="VEL04_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003268141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HYT48296.1"
                     /translation="MKRWGLAAALAVAMPLAAQDKPAASPAPPLTGKFGFVNTERILR
                     DAVPAVRAQKKIEAEFQKRDQELAKLAEQLKRMQDDIEKNSVTMSETQRRGKERELGE
                     LNRDFQRRQREFREDLNQRRNEELAQVVEQANRVIRQIAEQEKFDIIFQEMPAYVSPR
                     IDITDKVIKALDAGKPPAK"
     gene            complement(645..2921)
                     /gene="bamA"
                     /locus_tag="VEL04_07745"
     CDS             complement(645..2921)
                     /gene="bamA"
                     /locus_tag="VEL04_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HYT48297.1"
                     /translation="MHRGIAAVCAALLLVAAAGASAQSFQPFVVKDIRVEGLQRTEPG
                     TVFSYLPVKVGETMNEEKARQAVRALYATGFFQDVRLEADHEVLVVLVQERPAIAQVD
                     FAGMKEFEPDNIKKALRELGLAEGRIFDRALLDSAEQEMKRQYLSRGLYATEVQTTVT
                     PLERNRVAINITVTEGEVAKIRGINIVGAQAFEESELLDQLVLRTPGWLTWYTKHDRY
                     AREKLAADLETLRSFYQNRGYLDFSIESTQVAITPDRRDIYITVNLTEGEKYTVSGVR
                     ISGQLLLPREELEALVQLKTGDVFSREKLAASTKAIADRLGNDGYAFANANAIPEVDK
                     QKRTVAFNIVLDPGRRVYVRRIDIAGNVKTRDEVVRREMRQLEGAYYDASKIQLSRRR
                     IDRTAFFSEVSVETHPVEGSPDQVDVAYSVKERPTGALLFGIGFSNVEKVALSASLSQ
                     ANVFGTGNFLSLNVNSGKVNTVYSLSYLNPYFTVDGVSQGFDVYHRKTNASNLAIGAY
                     VTTALGGGVKFGYPISEVTRIDFGMNLESVKLDTFDNSPLQYINFVNQFGHQYSYGAL
                     TGGWSRDTRDSLIQTNAGAFSRMSTEFASGDLQYYRLGFQQQWYRPLTRTQTLYLAGD
                     IGYAGGLADKPLPFFKNYYAGGPGTVRGYSSFTLGPQDVNGTPIGGNRKFSGSAELLF
                     PMPGAVQDRSLRLAWFVDGGQVFADRYQFNSLRYSTGLGFFWSSPFGPLRLSFAQPLN
                     AKSTDHIQRLQFTFGTGF"
     gene            complement(2924..>3576)
                     /gene="rseP"
                     /locus_tag="VEL04_07750"
     CDS             complement(2924..>3576)
                     /gene="rseP"
                     /locus_tag="VEL04_07750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HYT48298.1"
                     /translation="GPAERAGLAPGDRVLSVDGIAVKTWDDFVNAVRSKPGVPLVLSI
                     DRAGATRTIEVIADPKTRRIGAGVRQPADYAAKMFVRVQYGPFASLARGVAKTADISI
                     FSLKILGKMLLGEVSWRHLSGPVTIADFAGQSAQMGWISYLSFLALISISLGVLNLLP
                     IPLLDGGHLMYYAIEIIKGRPVSERAMELGQRVGLALLLVMMAFAFYNDLNRLLTG"
BASE COUNT          579 a         1143 c         1192 g          662 t
ORIGIN      
        1 ctggcggatc agcgtctgcg ggtcgcctgc gacgcggccg ccgaggcgcg aggcgatctc
       61 cccgagcgtc aacggcgccg gcatcgcgtc acttcgcagg cggcttgccg gcgtccagcg
      121 ccttgatcac cttgtcggtg atatcgatgc gcgggctcac gtaagccggc atctcctgga
      181 agatgatgtc gaacttctcc tgctccgcga tctgccggat gacgcggttc gcctgctcga
      241 ccacctgcgc cagctcctcg ttgcggcgct ggttcaggtc ctcgcggaac tcgcgctgcc
      301 tgcgctggaa atcgcggttc agctcgccca attcgcgctc cttgccgcgg cgctgcgtct
      361 cgctcatggt caccgagttc ttctcgatgt cgtcctgcat gcgcttgagc tgctcggcca
      421 gcttggcgag ctcctggtcg cgtttctgga actcggcctc gatcttcttc tgcgcgcgta
      481 ctgccgggac cgcgtcgcgc aggatgcgct cggtgttgac gaagccgaac ttgccggtca
      541 acggcggcgc aggcgacgcc gccggcttgt cctgggctgc aagcggcatg gcgacggcga
      601 gcgccgcagc cagaccccat cgtttcatgc tttgcatagc caatttagaa ccccgtgccg
      661 aacgtgaatt gtagcctctg gatgtggtcc gtgctcttgg cgttcagggg ctgcgcgaag
      721 gacaatcgca acgggccgaa aggcgaagac cagaaaaagc cgagaccggt cgaatagcgc
      781 aggctgttga actggtagcg gtcggcaaac acctggccgc cgtcgacgaa ccacgccagc
      841 cgcagcgagc ggtcctgtac ggcgccgggc atcgggaaca gcagctcggc gctgcctgaa
      901 aacttgcggt tgccgcctat cggcgttccg tttacgtcct gcgggccgag agtaaacgag
      961 ctgtagccgc gcaccgtgcc ggggcctccg gcgtagtagt tcttgaaaaa cggcagcggc
     1021 ttgtcggcga gaccgccggc gtagccgatg tcccctgcca ggtatagcgt ttgggtgcgc
     1081 gtcagaggcc ggtaccactg ctgctggaac cccaggcggt agtactgcag gtcgccgctg
     1141 gcgaactcgg tgctcatgcg cgagaacgcg ccggcgttag tctggatcag gctgtcgcgg
     1201 gtgtcgcgcg accagccccc ggtcagcgcg ccgtaggaat actggtggcc gaactggttg
     1261 acgaagttga tgtattgcag cggactgttg tcgaaagtat cgagcttgac cgattccagg
     1321 ttcatgccga aatcgatgcg cgttacttcc gagatcgggt agccgaactt gaccccgccg
     1381 ccgagcgcgg tggtgacgta ggccccgatc gcgaggttgg acgcgttggt cttgcgatgg
     1441 tagacgtcga atccctggct gacgccgtcg acggtgaaat aggggttgag gtaggacagc
     1501 gagtacacgg tgttgacctt gccgctgttc acgttgagag acaggaaatt gcccgtgccg
     1561 aacacgttcg cctggctgag cgaggccgac agcgcaacct tttccacgtt cgaaaaaccg
     1621 atgccgaaaa gcagcgcccc ggtaggccgc tccttcacgc tgtaggcgac gtcgacctga
     1681 tccgggctgc cttccaccgg atgggtctcg acgctcacct cgctgaaaaa cgcggtgcgg
     1741 tcgatgcggc ggcgcgaaag ctggatcttg gaggcgtcgt agtacgcgcc ctcgagctgg
     1801 cgcatctcgc ggcgcaccac ctcgtcgcgc gtcttgacat tgccggcgat gtcgatgcgg
     1861 cgcacgtaaa cgcgccggcc cgggtcgagc acgatgttga acgccaccgt gcgcttttgc
     1921 ttgtcgacct cggggatcgc gttcgcgttg gcgaaggcat agccgtcgtt gccgaggcgg
     1981 tcggcgatcg ccttggtgct cgcagcgagc ttttcgcgcg aaaacacgtc gcccgtcttg
     2041 agctgcacca gcgcctcgag ctcttcgcgc ggcagcagca gctgcccgga gatgcgcacc
     2101 cccgagacgg tgtacttctc gccttccgta aggttgaccg tgatgtagat atcgcgccgg
     2161 tcgggcgtga tcgcgacctg cgtcgattcg atgctgaagt cgagatagcc gcggttctgg
     2221 tagaacgagc gcagggtctc caggtcggcg gcgagcttct cgcgcgcgta acggtcgtgc
     2281 ttggtgtacc aggtgagcca gccgggcgtg cgcagcacga gctggtcgag gagctcgctt
     2341 tcctcgaagg cctgcgcgcc gacgatgttg atgccgcgga tcttcgccac ctcgccctcg
     2401 gtgacggtga tgttgatggc gacccggttg cgctcgagcg gcgtgaccgt ggtctgcacc
     2461 tcggtcgcgt acagcccgcg cgacaggtac tggcgcttca tctcctgctc ggccgagtcg
     2521 agaagcgcgc ggtcgaagat acgcccctcg gccaggccca gctcgcgcag cgccttcttg
     2581 atgttgtccg gctcgaactc cttcatgccg gcgaagtcga cctgcgcgat ggccgggcgc
     2641 tcctgcacca gcaccaccag cacctcgtgg tcggcctcca ggcgcacgtc ctggaagaat
     2701 ccggtggcgt acagggcgcg caccgcctgc cgggctttct cctcgttcat cgtctcgccg
     2761 accttgaccg ggagatagct gaacaccgtg ccgggctcgg tgcgttgcag tccctcgacg
     2821 cggatgtcct tcaccacgaa cggctggaag gattgcgcgc tcgcgccggc ggccgccacg
     2881 agcaaaagcg cggcgcatac cgcggcgatg cctctgtgca tcttcagccg gtgagcaggc
     2941 gattgaggtc gttgtagaag gcgaaggcca tcatgaccag cagcagcgcc agaccgacgc
     3001 gctggccgag ctccatcgcg cgctcggaca cgggcctgcc cttgatgatc tcgattgcat
     3061 aatacatgag gtgtccgccg tcgagcagcg ggatcggcag caggttcagc acgccgaggc
     3121 tgatgctgat gagcgcgaga aagctgagat acgagatcca tcccatctgc gccgactggc
     3181 cggcgaagtc cgcaatcgtc accgggccgg agagatgcct ccaggacact tcgcccagca
     3241 gcatcttgcc gaggatcttg aggctgaaga tcgagatgtc tgcggttttg gcgacgccgc
     3301 gcgccaggct cgcgaacggg ccgtattgca cgcgcacgaa catcttggcg gcatagtctg
     3361 cgggctgccg tacccccgcg ccgatgcggc gcgtcttggg gtcggcaatg acttcgatgg
     3421 tgcgcgtggc acccgcccgg tcgatcgaca gcacaagcgg gacgccgggc ttcgagcgta
     3481 ctgcgttgac gaagtcgtcc caagtcttga ccgcgatgcc atcgacgcta agcactcggt
     3541 cacccggggc gaggcccgcg cgctcggccg gcccgc
//
LOCUS       DASPXW010000343         2441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_854437, whole genome shotgun sequence.
ACCESSION   DASPXW010000343 DASPXW010000000
VERSION     DASPXW010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854437"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VEL04_07755"
     CDS             <1..185
                     /locus_tag="VEL04_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48299.1"
                     /translation="TASAQQPGAAGSAAPGAAASSAKKGDRPKGGAAPQPIHGSFPGP
                     SKQPPLKLDPRASAKR"
     gene            complement(199..>2441)
                     /locus_tag="VEL04_07760"
     CDS             complement(199..>2441)
                     /locus_tag="VEL04_07760"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:NF002837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HYT48300.1"
                     /translation="LEIGHSVRTVPGCVAAAAGDVTIATTLLESRYLVGSRTLYRALH
                     AALGRVADPASFFKAKKLEQEQRHAKHQDSPYSLEPNLKEAPGGLRDLQVVQWIARAS
                     GIGRRWSDLVAHGVIEPREARQLARHEAVLQDLRIRLHYLAGRREDRMVFDFQGALAA
                     ELGCAATPDRRASEVMMQRYYRTAKAVTQLNTIVLQNLEARLLPQPDAVPRALNERFQ
                     VRGELLEVTDETAFEKHPTTILESFLLLMQHQELRGMTAPTLRALYRARFSINSRIRK
                     DPLARLLFVHILQQPRGIVHELRRMNQYGILGRYLPEFGRIVGQMQHDLFHVYTVDQH
                     ILMVVRNLRRFTMPEFAHEFPLCSELMSGFERRWLLYVAALYHDIAKGRGGDHSELGA
                     TEARRFCRTHALSEEDSKLLEFLVERHLAMSHAAQKQDVHDPEVVRAFAEVVQSERRL
                     VALYLFTVADVRGTSPKVWNAWKAKLLEDLFRATRRVLTGEPLARDAALAEKQAEAAR
                     LLRLYALDDSVKDRLWAQLDTAYFLRHDAQEIAWQTRTLHYRVESDRPVVKARLAPFG
                     EGLQVMIYTKDREALFARICGYFERAGFNIAEAKIHTTRSGYALDTFVVMGEGRDAHY
                     QDRIAMIEAQLAEEVVSEAPLAPPRGGRLSRRARHFPISPAVDIRPDERGAYHVLSIV
                     ASDRPGLLYGVARLLARYRINLQTARINTLGDRAEDVFLVSGEALSAPKTVLQLEQDL
                     LKELQI"
BASE COUNT          391 a          808 c          848 g          394 t
ORIGIN      
        1 cgacagcttc cgcccagcaa ccgggcgccg ccggcagcgc agcgccgggt gccgccgcga
       61 gcagcgcgaa gaaaggcgac cgccccaagg gcggcgccgc gccccagccc atccatggca
      121 gctttcccgg cccctccaag cagccgccgc tcaagctcga tccgcgcgct tcggcgaagc
      181 gctgacacag aagagcgact agatctgcaa ttccttcagc aagtcctgct cgagctgcaa
      241 gaccgtcttc ggcgcggaca aggcctcgcc tgagacgagg aagacgtcct cggcgcgatc
      301 gccgagcgtg ttgatgcggg cggtctggag gttgatgcgg tagcgtgcga ggaggcgggc
      361 gactccatag aggaggccgg ggcggtcgct cgcgacgatc gagaggacgt ggtaggcgcc
      421 gcgttcgtcg ggccgtatat cgaccgcggg cgagatcggg aagtggcgcg cgcggcgcga
      481 caggcgcccg ccgcgcggcg gggcgagcgg cgcctcggag accacctcct cggcgagctg
      541 ggcctcgatc atggcgatgc ggtcctggta gtgcgcgtcg cggccttcgc ccatgacgac
      601 gaaggtgtcg agcgcgtagc ccgagcgcgt ggtgtggatc ttggcttcgg cgatgttgaa
      661 gcctgcgcgc tcgaaatagc cgcagatgcg cgcaaacagg gcctcgcggt ccttggtata
      721 gatcatcacc tgcaggccct cgccgaacgg tgcgagccgc gccttgacca ccgggcggtc
      781 gctctccacg cggtagtgca gggtgcgcgt ctgccaggca atttcctggg cgtcatgacg
      841 caggaagtaa gccgtgtcga gctgcgccca cagccgatcc ttgacgctgt cgtcgagcgc
      901 gtacaggcgc agcaggcgcg ccgcctcggc ctgtttctcg gcgagcgccg catcgcgcgc
      961 gagcggctct ccggtgagca cccgccgcgt ggctctgaac aggtcctcga gcagcttggc
     1021 tttccacgcg ttccacacct tggggctggt gccccgcaca tcggcaacgg tgaacaggta
     1081 aagcgcgacc agccgccgct cgctctgcac cacctcggca aacgcgcgta ccacctccgg
     1141 atcgtgtacg tcctgcttct gcgcggcatg cgacatggcg aggtggcgct cgaccaggaa
     1201 ctcgagcagc ttgctgtctt cctcggacaa ggcatgcgtt ctgcagaacc tgcgagcctc
     1261 cgtggccccc agctccgagt ggtcgccgcc gcggcccttg gcgatgtcgt gatacagcgc
     1321 ggcgacatac agcagccagc ggcgctcgaa gccgctcatc agctcgctgc agagcggaaa
     1381 ctcgtgcgcg aactcgggca tggtgaagcg ccgcagattg cgcaccacca tcaggatgtg
     1441 ctgatcgacc gtgtagacgt gaaacagatc gtgttgcatc tgcccgacga tgcgaccgaa
     1501 ttcgggcagg tagcgcccca ggatgccgta ctggttcatg cgcctgagct cgtgcacgat
     1561 gccgcgcggc tgctgcagga tgtgaacaaa cagcaggcgc gcgagcgggt cttttctgat
     1621 ccgggaatta atcgagaacc gggcgcgata cagggcgcgc agcgtcggcg ccgtcatgcc
     1681 tcgcagctcc tggtgctgca tcaggagcag aaagctttcc aggatggtgg tcgggtgttt
     1741 ttcaaacgcg gtttcgtcgg tcacctcgag aagctcgccg cgcacctgga agcgctcatt
     1801 caatgcacgc ggcacggcgt cgggttgggg caggagccgc gcttcgaggt tctgcagcac
     1861 gatcgtattg agctgggtga cggcctttgc cgtgcggtag tagcgctgca tcatcacctc
     1921 gctcgcgcgg cgatccgggg tggcggcgca gccgagctcg gcggcgagcg ccccctggaa
     1981 atcgaacacc atgcggtctt cgcggcggcc ggcgaggtaa tgcaggcgaa tcctgaggtc
     2041 ctgcagtacc gcctcgtggc gcgcgagctg gcgcgcctcc cggggctcga tcacgccgtg
     2101 cgccaccagg tcgctccagc gcctgccgat gcccgaggcg cgggcgatcc actggactac
     2161 ctgcaagtcc cttagtcccc cgggcgcctc cttcaggttg ggctcgagcg agtagggcga
     2221 gtcctggtgc ttggcgtggc gctgctcctg ctcgagtttc ttcgccttga agaacgacgc
     2281 cgggtcggcg acgcgcccga gcgccgcgtg cagggcgcgg taaagcgtgc ggctccccac
     2341 gaggtagcgg gactcgagca gtgtggtggc gatagtaacg tcgcccgccg cggccgcgac
     2401 gcaacccggc acggtacgca cgctgtgccc gatctcaagc c
//
LOCUS       DASPXW010000344         4281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_103657, whole genome shotgun sequence.
ACCESSION   DASPXW010000344 DASPXW010000000
VERSION     DASPXW010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4281
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103657"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VEL04_07765"
     CDS             <1..315
                     /locus_tag="VEL04_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S11"
                     /protein_id="HYT48301.1"
                     /translation="VRTAHEYPLIREYSTHERSSVDVYGRPMSFRNTNGLVRSSQWDI
                     GLSKTGYISEAGRCLVMRVHLASKDLIVVLLSSWGKQSRVGDANRIRRWLETHATLGR
                     TS"
     gene            complement(325..1239)
                     /gene="egtD"
                     /locus_tag="VEL04_07770"
     CDS             complement(325..1239)
                     /gene="egtD"
                     /locus_tag="VEL04_07770"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HYT48302.1"
                     /translation="MGSVPIFLEDVIAGLSLPQKSLPPKYFYDAAGSRLFERICRLPE
                     YYLTRAELALTRAHLAAIARFAGRGCELVEYGSGASHKSRLLIRALRPKAYLPVDISA
                     SALEQAAARLAREFPWLRIVPVRGDFSRPLEIPAGARGRRVAYFPGSTIGNLTPEEAH
                     AFLARSRGQAGRMLVGTDLKKDANVLHAAYNDARGVTAAFNLNLLARINRELGGDFDL
                     KRFSHYAFYNAALGRIEMHLVSLEKQTVRLGGHSFGFQRGESIHTENSYKYAAQEFRA
                     LAARAGFKAKKAWTDREARFALHALAGD"
     gene            complement(1283..2077)
                     /locus_tag="VEL04_07775"
     CDS             complement(1283..2077)
                     /locus_tag="VEL04_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004524720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HYT48303.1"
                     /translation="MSTPRDEPKNPIQVIERMLRLLEVLAQHPEPLGLKQISQYTGLH
                     PSTAHRILSSMSADRIVDRVEPGSYRLGMRLLELGNLVKSRISVRELALPRMRELHSH
                     TGETANLSVRHDDQIVYVERTSSGRSAMRVVHVIGARAPLHVTAAGKLFLLEEGFARL
                     RDYAKRTGLAPQTKNTLTQLVLLERDLERIQQQGWATDNEEAEIGVRCVAAGIRDDAG
                     RLVAALSLSTPADRMKLQWGPLVKETADRISQNIGHQPPLAKIGAH"
     gene            2117..3256
                     /locus_tag="VEL04_07780"
     CDS             2117..3256
                     /locus_tag="VEL04_07780"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HYT48304.1"
                     /translation="MPVVTCIEDLREMAKKRVARAIFDYVDCGSYSEFTLRANRADFE
                     AIALRQRVGIDVERRSTRTTMAGQEAAMPVALAPTGLTGLNWADGEMLAARAAERFGV
                     PFTLSTMSICSIEDVAGAVTRPFWFQLYVMRDRGFAASLIERAKLAKCSALVLTLDLQ
                     IQGQRHRDLKNGLAVPPRLTFGTFLDVLSKPSWALNVLRGRRKSFGNLEGRIPDANSL
                     TTLSQWIAGQFDPTLSWKDVEWVKDRWGGKLVLKGILDAEDAKIAASSGADAIVVSNH
                     GGRQLDGTISSIRALPEVVDAVGDRMEVWLDGGIRSGQDVLKALALGARATMIGRAFL
                     YGLGAMGEAGVTRALEIIRAELDVSMALCGLRDVKDASRSMLRAL"
     gene            complement(3219..>4281)
                     /gene="mnmC"
                     /locus_tag="VEL04_07785"
     CDS             complement(3219..>4281)
                     /gene="mnmC"
                     /locus_tag="VEL04_07785"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HYT48305.1"
                     /translation="RKAIVVGAGLAGAAVCERLCARGWEVELIERRAGPAQEASGNHA
                     GAFHPIVTPDDSVFARLTRAGFLYSIFRWSLLEKPKWDRCGVLQLARNDKEAASQQKS
                     VLGLPPEYAQLVTREEAAAHAGAPVAAGGLWFPEAGWIRPQTLVRAQLEACGSRLHVR
                     YGFEVDSIPEAAVVILANSFDAPKLLAVPHLRLRRVRGQLTHVPAESIEAPRVVVLRG
                     GMVLPPVEGLCVVGASYDLEDEDTATREDSHAGNLQRLANIIPFDATLKNLQGRVAFR
                     AVTPDRLPVVGKIAEGVYGAFAYGSRGLVWAALAAELIASELEGEPPPLESSLSRALH
                     PGRFGLRAKARAASSGSRP"
BASE COUNT          677 a         1463 c         1462 g          679 t
ORIGIN      
        1 gtgcgcacgg cgcacgagta cccgctgatc cgcgaatact cgacgcacga gcgcagcagc
       61 gtcgatgtct acgggcggcc gatgtcgttc cgcaacacca atggcctggt gcgcagctcg
      121 cagtgggata tcgggctgtc gaaaaccggc tacatcagcg aggcgggacg ctgcctcgtg
      181 atgcgggtgc atctcgcttc gaaggacctg atcgtggtgc tgctcagctc gtggggcaag
      241 caatcgcggg tcggcgacgc caaccgcatc cgcaggtggc tcgaaaccca cgccacgctc
      301 ggccgcacca gctagccggc caggtcaatc gcccgccaag gcgtgaagcg cgaagcgcgc
      361 ctcacgatcg gtccacgcct ttttcgcctt gaatcccgcc ctcgcagcca gcgcgcggaa
      421 ctcttgcgcc gcgtacttgt aggaattctc ggtgtggatc gactcgccgc gctgaaagcc
      481 gaagctgtgc cccccgagcc gcacggtttg cttctccagg gaaaccaggt gcatctcgat
      541 gcggccgagc gcggcgttgt agaaggcgta gtgcgagaag cgcttcaggt cgaaatcgcc
      601 gccgagctcg cggttgatgc gcgcgagcag attcagattg aaggccgcgg tcacgccgcg
      661 cgcgtcgttg taggcggcgt gcagcacgtt ggcgtccttt ttcaggtccg ttcccaccag
      721 catgcggccc gcctggccgc ggctcctcgc caggaaggcg tgcgcctcct cgggggtcag
      781 gttgccgatg gtcgatccgg gaaagtaggc gacgcgccgc ccgcgcgcgc ccgccgggat
      841 ctcgagcggg cgtgaaaaat cgccgcgcac cggcacgatg cgcagccagg gaaactcgcg
      901 cgcgagccgc gcggcggcct gctcgagcgc gctcgccgaa atatccaccg gcaggtaagc
      961 cttcggccgc agcgcgcgga tgagcagccg ggatttgtgg ctcgcgccgc tgccgtattc
     1021 gaccagctcg cagccgcggc ccgcgaagcg cgcgatcgcc gccaggtgcg cccgggtcag
     1081 cgccagctcg gcgcgcgtca ggtaatactc gggcaggcgg cagatgcgct cgaacaggcg
     1141 gctgcccgca gcgtcgtaga agtatttcgg cggcagcgac ttctgcggca gcgaaagccc
     1201 cgcgatgacg tcctccaaaa aaatggggac agaccccatt tttctcatct gcaattgcga
     1261 aaaatggggt ctgtccccat tttcagtgcg cgccgatctt cgccaggggc ggctgatggc
     1321 cgatgttctg cgagatgcgg tccgcggttt ccttgaccag cggtccccac tgcagcttca
     1381 tgcggtcggc cggggtcgag agcgagagtg cggcgaccag cctgccggcg tcgtcgcgga
     1441 tgcctgcggc gacgcagcgc acgccgatct cggcctcctc gttgtcggtg gcccagccct
     1501 gctgttggat gcgctcgagg tcgcgctcga gcagcaccag ctgcgtgagc gtgttcttcg
     1561 tctgcggcgc gagcccggtg cgcttcgcgt agtcgcgcag ccgcgcgaag ccttcctcga
     1621 gcagaaacag cttacccgcg gcggtgacgt gcagcggggc gcgcgcgccg atgacgtgca
     1681 ccacgcgcat cgccgagcgg cccgacgagg tgcgctcgac gtagacgatc tggtcgtcgt
     1741 ggcgcaccga gaggttcgcc gtctcgccgg tgtgcgaatg cagctcgcgc atcctgggca
     1801 gcgccagctc gcgcacgctg atgcgcgatt tcaccaggtt gccgagctcg agcaggcgca
     1861 tgccgagacg gtagcttccc ggctcgacgc ggtcgacgat gcggtctgcg gacatcgacg
     1921 acaggatgcg gtgcgcggtc gagggatgca gccccgtgta ctgcgagatc tgcttcaggc
     1981 cgagcggctc gggatgctgc gccagcacct cgagcagccg cagcatgcgc tcgatcacct
     2041 ggatcgggtt cttcggctcg tcgcggggcg tgctcatccg aatctcatcc gagttcgcat
     2101 tgtagaatgc cgcgccatgc cggtggtgac ctgcatcgag gacctgcgcg agatggcgaa
     2161 gaagcgcgtc gcccgggcga tcttcgatta cgtcgactgc ggctcgtaca gcgagttcac
     2221 gctgcgcgcc aaccgcgccg acttcgaagc gatcgcgctg cgccagcgcg tcggcatcga
     2281 cgtcgagcgg cgctcgacgc gcaccacgat ggccggccag gaagcggcga tgccggtggc
     2341 gctcgcgccg accggcctca ccggcctcaa ctgggcggac ggcgagatgc tcgcggcgcg
     2401 cgccgccgag cgcttcggcg tgccgttcac gctttccacc atgtcgatct gctcgatcga
     2461 ggacgtggcc ggcgcagtaa cccggccatt ctggttccag ctctacgtga tgcgcgaccg
     2521 cggcttcgcc gcctcgctca tcgagcgcgc gaagctggcg aaatgctccg cgctggtcct
     2581 gacgctcgac ctgcagatcc agggccagcg ccaccgcgac ctgaagaacg ggctcgcggt
     2641 tcctccgcgc cttacgttcg gcacctttct cgatgtgctg agcaagccgt cgtgggcgct
     2701 caacgtcctg cggggaagac gcaaatcctt cggcaacctc gaagggcgca tccccgacgc
     2761 gaacagcctc accacgctct cgcagtggat tgccggacag ttcgatccca cgctgagctg
     2821 gaaggacgtg gagtgggtga aagaccggtg gggcggcaag ctcgtgctca agggcatcct
     2881 cgacgccgag gacgcgaaga tcgccgcctc cagcggcgcc gacgccatcg tggtgtcgaa
     2941 ccatggcggc aggcagctcg acggcaccat ctcgtcgatc cgcgcgctgc ccgaggtggt
     3001 cgacgcggtg ggcgatcgca tggaagtctg gctcgacggc ggcatccgca gcggccagga
     3061 cgtgctgaag gcgctggcgc tcggcgcccg cgccacgatg atcggccgcg ccttcctcta
     3121 cggcctgggc gcgatgggcg aggccggcgt gacccgcgcg ctcgagatca tccgcgccga
     3181 gctcgacgtc tcgatggcgc tgtgcgggct gcgggacgtc aaggacgcga gccgctcgat
     3241 gctgcgcgcg ctttagcccg caggccgaag cggcccggat gcagggccct tgagagcgag
     3301 ctttcgagcg gcggcggctc gccttcgagc tcgctcgcaa tcagctcggc ggcgagcgcc
     3361 gcccacacca gtccgcgcga gccgtaggcg aacgcgccgt agacgccctc ggcgatcttg
     3421 ccgaccaccg ggaggcgatc cggcgtcacg gcgcggaacg cgacccggcc ttggagattt
     3481 ttcagcgttg catcgaacgg gatgatgttc gcgaggcgct gcaggttgcc ggcgtggctg
     3541 tcctcgcgcg tcgcagtgtc ctcgtcttcc aggtcatagc tcgcgccgac cacgcacagg
     3601 ccctcgaccg gtggcagcac catgccgccg cgcagcacca cgacgcgcgg cgcctcgatg
     3661 ctctccgccg gcacgtgagt gagctggccg cgcacacgac gcagcctgag gtggggcacc
     3721 gcgaggagct tcggtgcatc gaacgagttg gcgaggatga cgaccgccgc ttctggtatt
     3781 gaatcaactt caaatccgta acgaacgtga aggcgactgc cacaagcctc gagctgcgcc
     3841 cggaccagcg tctgcggccg gatccatccc gcctccggga accacaaccc gcccgccgcg
     3901 accggcgcgc cggcgtgcgc cgcggcttcc tcgcgcgtca cgagctgcgc gtattcgggc
     3961 ggcaggccga ggacggattt ctgctgcgaa gccgcttcct tgtcgttgcg tgccaattgc
     4021 agcacgccac agcgatccca tttcggtttc tcgaggagag accatctaaa aatggaatac
     4081 aagaaccccg cacgcgtcag gcgcgcgaac acgctgtcgt caggtgtgac gatgggatgg
     4141 aaggccccgg catggttgcc cgaagcttcc tgagccgggc cggcgcgccg ctcgatcagc
     4201 tcgacttccc agcctcgcgc acacaggcgc tcgcataccg cggcgcccgc gaggccggcg
     4261 ccgacgacga tggcctttcg c
//
LOCUS       DASPXW010000345        10791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1488060, whole genome shotgun sequence.
ACCESSION   DASPXW010000345 DASPXW010000000
VERSION     DASPXW010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10791
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1488060"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /locus_tag="VEL04_07790"
     CDS             <1..1060
                     /locus_tag="VEL04_07790"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HYT48306.1"
                     /translation="IAGNKLALAAYSPQSQIRARVWTFDHEEEVDAAFFRGRLTRALA
                     LREGLPAAKHTNALRLVHGESDGLPGLVVDRYADVLVVQILSAGIERWRDPILDALAE
                     SSGCEAIYERSDAEVRGLEGLEPRTGFVRGNRNASRCPISEYGLNFRVDVEQGQKTGF
                     FLDQRENRQRVRALAAGREVLDGFCYTGGFSIAALAGGAKRVTAIESSSSALEVAKEN
                     LAANPLDASKVEFLQADVFSHLRTLRDRGAHFDMVVLDPPKFAPTAAQAKNAARAYKD
                     INLLALKLLSPGGLLATFSCSGGVSAELFQSIVAGAALDAGAEAKIVERFTAAADHPV
                     ALEFPEGEYLKGLLVLRS"
     gene            complement(1050..2630)
                     /locus_tag="VEL04_07795"
     CDS             complement(1050..2630)
                     /locus_tag="VEL04_07795"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYT48307.1"
                     /translation="MNRHRLLEQVMHHYRVGALEEARRACEQILRVHAKDADALNMMA
                     AIAADERKTDEGMRWARLAAAADPAKPGPHYSMGRLCQGEGRLAEAEAHYRRSLALKP
                     DQPQALNNLGSVLHMQGRLDLAVASYRRALELDPTLPQANQNLAAITRELPAAERAVA
                     GFLRQLRENPKDAQAHTNLGNVYRELGRHREAIASFANALACDPELAEAHFSRAEELL
                     LCGEFLEGWKEYEWRWKVKGLRTPPRDIAQPFWNGEDLGSGTLLLHPEQGLGDTLQFA
                     RYAPMAAGRCGRIVLECVPSLMGLLRALPGVSQVVAAGEPLPPFDAHLSLMSLPRVFQ
                     TTLRDIPWRGPYVQAEASRAERWRDTLRADSRIRVGLVWAGRPEHWNDRNRSIALSTL
                     APLAMVQEATFYSLQKGEAASQARTPPAGMKLVDLTAGIADFADTAALLGQLDLLISV
                     DTSVAHLAGAMGVATWLLLPHAPDWRWLLEREDSPWYPSLRLFRQKADGDWPGVIERV
                     ALALTQVMQQRPGRRVSS"
     gene            2690..3655
                     /locus_tag="VEL04_07800"
     CDS             2690..3655
                     /locus_tag="VEL04_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HYT48308.1"
                     /translation="MVPVTILTGFLGSGKTTLLNRILREDHGRRIAVIENEFGEVGVD
                     SALIEKSDEQIVEMNNGCICCTVRGDLIRILGSLKDKRDQGALKFDRVVIETTGMADP
                     GPVAQTFFTDEEIGNYYLLDSIITIVDAKHAPQQLDEFHEAQEQVGFADRILLSKTDL
                     TEEAETELLIKRLKKMNPRAPIKKVSFGDAPLDEVLDIRGFNLNAILELDPEFLTDVQ
                     HEHHDQVESFVFRAKKPFDGTKLEQFLSGMIQVYGPDLLRYKGVLWMKGNPRRVVFQG
                     VHMMMGGDLGKPWTRADKKESLLVFIGKKLPKDLFIAGLEECLAK"
     gene            3663..3995
                     /locus_tag="VEL04_07805"
     CDS             3663..3995
                     /locus_tag="VEL04_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48309.1"
                     /translation="MSLRRYAVLRIVCAFLLLLAQQGALTHDIWHSAKAASHADVSIA
                     GDAKDVKKNRLCELHAALGSVLGALGGNAPALAVADTNEIGFKTADASAIIVPPLPPA
                     SRGPPTLL"
     gene            4053..5312
                     /locus_tag="VEL04_07810"
     CDS             4053..5312
                     /locus_tag="VEL04_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48310.1"
                     /translation="MYRNMWCAAALAAAMLPAQAQDVKQLERRVQELEQRVAENTPKQ
                     SSNAFNPDISVILQGTAARSSQDPANFQITGFAPAGGEVAPARRGFSLGESELVFASN
                     IDPYFRGQLVAALAPDDAVEVEEAFFQTLRLGRGLTVKGGRFLSGIGYQNPIHQHAWD
                     FQDAPLAYKAFLGGRLNDDGVQLKWVAPSELLVELGSELGRGRSFPATDPNKNGAAAW
                     TLFGHAGGDFGASAAWRAGLSHLRTSPRERTFDDVDTSGNPVTQSFTGSSRLWIADFV
                     LKWAPDGNSTVTNFKLQGEFFRRSESGELTFNNAMTGDYASRQSGWYLQGVYQFMPQW
                     RAGYRYDRLSHGSVSNGLGLTAGDSPLLLTEHNPTRHTLMLDWSATEFSRIRLQLASD
                     KSRAGVTDNQVLLQYIYSLGTHGAHTF"
     gene            5323..6216
                     /locus_tag="VEL04_07815"
     CDS             5323..6216
                     /locus_tag="VEL04_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HYT48311.1"
                     /translation="MRFLLTFLLFVSSWPACAALNILACTPEWGALAKELGGEKVSVY
                     VASTALQDPHRIEARPSLIARARSADLMVCTGAELEIGWVPLLQTQSGNEKIQVGAPG
                     FFEAASVVPLLERPNRLDRSMGDVHPGGNPHIHLDPRNVAKVAAALGERMAALDPREA
                     AYYSDRSNSFLERWEQARIRWEKEAIPLKGLPLVVYHKNMTYMIHWLGMREVGNLEPK
                     PGLPPTTAHLSELLANLQRQPAKAVVRSAYNDPRPGEWLAERAKIPSVTVAFTVGGTP
                     EAKDLFALYDDTLARLLAVAK"
     gene            6213..6974
                     /locus_tag="VEL04_07820"
     CDS             6213..6974
                     /locus_tag="VEL04_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HYT48312.1"
                     /translation="MSAATLDLSIVWPALVAGLLVTATHVPLGIQVLKRGIVFIDLAV
                     AQIAGLGVVFADWLGFEPHGAAVQAAALSAALGGALLLNWTDKRWPEVQEAVIGVTFI
                     VAANAAILLLAANPRGGEHLKDLLIGQILWVRPVELPVVAAVSAAIVAIWFGAGQRIG
                     RIGFYLLFACAVTASVQLVGIYLVFTTLIAPALATRHLARHRLASCYVLGALGYAVGL
                     GLSLVTDLPPGPLIVCAMTALGIALAIATSQRAAT"
     gene            complement(6984..7499)
                     /locus_tag="VEL04_07825"
     CDS             complement(6984..7499)
                     /locus_tag="VEL04_07825"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HYT48313.1"
                     /translation="MKPQVMIAAWLLAGAAHAQHQPYAGQHEREIKALSADEVKQYLS
                     GAGMGYAKSAELNHYPGPGHALEFADKLGLSAEQRAAAKALASAHHAEARAIGAKLVD
                     SEYGLEQLFRSGRVDEAALARAVRTAAQLAGDYRLSHLETHRRTRALLTDEQVRRYDE
                     LRGYAGTRHAH"
     gene            complement(7517..8326)
                     /locus_tag="VEL04_07830"
     CDS             complement(7517..8326)
                     /locus_tag="VEL04_07830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HYT48314.1"
                     /translation="MDNAFLLEHEAPIRVTAMALALTGMAAWEVFAPRRSPRESKARR
                     WRTNLGVALLNVLLVRVALPTTAIGIAELAAARGWGLFNQLEIESWLAVVIAVLALDL
                     FIYLQHVAFHLTPVLMRIHAVHHADPDVDATTGIRFHPIEIILSMLLKYAVIVALGTP
                     AVAVLVFEMLLGVSSLFNHGNVRLPEIIDRALRFVLVTPDMHRIHHSVIEAERNSNYG
                     FCISAWDRLFGTYTLSPAGGQTGMRLGLDSYRDAWVSTTLSGALAIPFRTW"
     gene            8407..9072
                     /locus_tag="VEL04_07835"
     CDS             8407..9072
                     /locus_tag="VEL04_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HYT48315.1"
                     /translation="MHKSSDPEKGRLYEAIGRVAAALGSAGRLQILELVAQGERSVDA
                     LAAMTGLSVANTSKHLQALRQAGLVSARKQGLRVYYVLAGDDVTALVAALRAVAEHRA
                     ADVERLVRTWLAHRDELEPVPAAELLQRAKKGLVTVLDVRPAEEYAAGHVPGAINVPV
                     DKLESYLSKLPKRKEVVAYCRGPYCLMSFDAVEKLRKRGWRARRLQDGFPEWRAAGLP
                     VET"
     gene            complement(9075..9545)
                     /locus_tag="VEL04_07840"
     CDS             complement(9075..9545)
                     /locus_tag="VEL04_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48316.1"
                     /translation="MTAAGWYRDSAARSLILRGYLPWLAALSLAWEILQLPLYTIWSE
                     AAAGYIAFALAHCTAGDVLTGLGSLALALLATRAGGMETWNWQAVAFLLAVFGVAYTG
                     FSEWTNTVLRPAWAYSQLMPTLRVAGVELGLSPLLQWLAVPPLALYLARSTRAA"
     gene            complement(9553..10005)
                     /locus_tag="VEL04_07845"
     CDS             complement(9553..10005)
                     /locus_tag="VEL04_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HYT48317.1"
                     /translation="MPLRRIALALTLAACTESPSPGDDARADPRNAAKVALGAKVYAQ
                     HCAACHGARLEGQPNWRIRLPNGRLPAPPHDDSGHTWHHSDQLLFGITKRGLVPPYAP
                     AGYESDMPAFGGKLSEDEIWAVLAYIKSRWTQEVLPLRAEMTRNARKR"
     gene            complement(9996..10640)
                     /locus_tag="VEL04_07850"
     CDS             complement(9996..10640)
                     /locus_tag="VEL04_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012435795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HYT48318.1"
                     /translation="MPGSAALGAGDAKRGAQLFGQCMACHSVEPGEHMTGPSLAHIWN
                     RKAGTVDGFQRYSEPMKRLGITWNEAALDKWLANPEKFLPGTSMTFPGLKERKDREDM
                     AAYLKAVAENKAPQTGGGMMGMRGTKLDLRKAPPEGQVRSIRYCGDAYTVETADGKRQ
                     KIWEFNLRFKSDSTKLGPEPGKPIVVGAGMQGDRASIVFASPKEISEFVKASCP"
BASE COUNT         1853 a         3545 c         3537 g         1856 t
ORIGIN      
        1 catcgcgggg aacaagctgg ccctcgccgc ctacagcccg cagtcgcaga tccgcgcacg
       61 cgtctggacc ttcgaccacg aagaagaagt cgatgcggcg ttttttcgcg ggcgcctgac
      121 gcgcgcgctc gccttgcgcg aagggctgcc ggcggcgaag cacaccaacg cgctgcggct
      181 ggtgcacggc gaatccgacg ggctgccggg gctcgtcgtc gaccgctacg ccgacgtgct
      241 ggtggtgcag atcctctcgg cgggcatcga gcgctggcgc gacccgatcc tcgacgcgct
      301 ggcggaatcg agcggctgcg aagccatcta cgagagatcg gatgccgagg tgcgcggcct
      361 ggaagggctg gagccgagaa cgggattcgt gcgcggcaat cgcaacgcct cgcgctgccc
      421 gatcagcgaa tacggcctca acttccgcgt cgacgtcgag caggggcaga aaaccggctt
      481 cttcctcgac cagcgcgaga accgccagcg ggtgcgcgcg ctcgccgccg gacgcgaggt
      541 gctcgacggc ttctgctata cggggggctt ttcgatcgcc gcgctggcgg gcggcgcgaa
      601 acgggtcacc gcaatcgaaa gttcttcaag tgccctggag gtcgcaaagg aaaatctcgc
      661 cgcgaatccg cttgacgcct cgaaggtgga attccttcag gccgacgtct tttcccatct
      721 gaggaccctc agggatcgcg gcgcgcattt cgacatggtg gtgctcgatc cgcccaagtt
      781 cgcgcccacc gcggcgcagg cgaagaacgc cgcgcgagcc tacaaggaca tcaacctgct
      841 cgccctcaag ctgctctcac cgggcgggct gctcgcgacc ttctcgtgct cgggcggggt
      901 gagcgcggag ctcttccagt cgatcgtcgc cggcgccgcg ctcgacgccg gcgccgaggc
      961 aaagatcgtc gagcgcttta ccgccgccgc agaccacccg gtggcgctcg aattccccga
     1021 gggcgaatac ctaaaagggc tgctggtcct caggagctga cgcgacgccc gggccgctgt
     1081 tgcatgacct gggtgagcgc gagggcgact cgctcgatca cccccggcca atcgccgtcc
     1141 gctttctggc ggaacaagcg caggcttgga taccatggac tgtcctcgcg ctcgaggagc
     1201 cagcgccagt cgggggcgtg cgggagcagc agccacgtgg cgacacccat ggcgccggcg
     1261 agatgggcga ccgacgtgtc gacggagatg agcaagtcga gctgaccgag cagcgccgcc
     1321 gtatcggcga aatccgcgat gcccgcggtc aggtcgacca gcttcatgcc ggcgggcggg
     1381 gtcctggcct gggacgcggc ttcgcccttc tgcaggctat agaacgttgc ctcctgaacc
     1441 atggcaagcg gcgccagcgt cgaaagcgct atcgagcgat tgcggtcgtt ccaatgctcg
     1501 ggtcgcccgg cccagaccag gccgacgcga atccgcgaat cggcgcgcag ggtgtcccgc
     1561 caccgctcgg cgcgcgacgc ttctgcctgg acgtacggcc cgcgccaggg gatgtcgcgc
     1621 aaagtcgtct ggaatacgcg cggcaggctc atcagcgaaa gatgagcgtc gaacggcggc
     1681 agcggctcgc ctgcagcgac cacctgcgag acacccggca gcgcgcgcag cagccccatc
     1741 agggacggca cgcactcgag gacgatcctg ccgcagcggc ctgcggccat cggcgcataa
     1801 cgggcgaact ggagggtatc gccgagtccc tgctccgggt gcaggagcag cgtgccgctt
     1861 cccaggtctt cgccgttcca gaaaggttgc gcgatgtccc ggggcggcgt gcgcagccct
     1921 ttcactttcc agcgccactc gtattccttc cagccctcga ggaattcgcc gcaaagaagc
     1981 agctcctcag cgcggctgaa atgcgcttct gcaagctccg gatcgcacgc cagcgcgttg
     2041 gcgaaggagg caatggcttc ccgatgccgt cccagttcgc gatagacgtt gccaagattg
     2101 gtgtgtgctt gcgcgtcttt cgggttctct cgtaactgac gcagaaaacc cgcaaccgct
     2161 ctctcggccg cgggcagctc gcgggtaatg gcggcgaggt tctggttggc ctggggcagt
     2221 gttggatcca gctcaagtgc ccggcgatag cttgctaccg cgaggtcgag cctgccttgc
     2281 atgtgcagca cggaaccgag attgttgagg gcctgcggct ggtcgggctt cagggcgagc
     2341 gagcgcctgt agtgcgcctc ggcttcggcg aggcggcctt ccccctggca cagccgtccc
     2401 atcgagtagt gaggacccgg cttggctgga tcggcggccg cggcaaggcg cgcccagcgc
     2461 atgccttcat cggtctttct ttcgtcggca gcaattgccg ccatcatatt gagagcgtca
     2521 gcgtccttgg catgcacgcg caggatttgc tcgcacgcgc ggcgcgcttc ttccagggct
     2581 ccgacccggt agtgatgcat cacctgctcc agtaaccgat ggcggttcat cgagtccggc
     2641 cgggttaaaa tttcactctt tccagggaga cggcatgcag gaagagcgca tggttcccgt
     2701 aaccattctc acgggattcc tcggcagcgg caagaccacg ttgctcaacc gcattctcag
     2761 ggaagaccac ggccggcgca tcgcggtgat cgaaaacgag ttcggcgagg tcggggtcga
     2821 cagcgcgctg atcgagaaga gcgacgagca gatcgtcgag atgaacaacg gctgcatctg
     2881 ctgcaccgtg cgcggcgacc tgatccgcat cctcggcagc ctcaaggaca agcgcgacca
     2941 gggcgcgctc aagttcgacc gcgtggtgat cgagaccacc ggcatggccg accccggccc
     3001 ggtagcgcag accttcttta ccgacgagga gatcggcaac tactacctgc tcgactcgat
     3061 catcaccatc gtcgacgcca agcacgcgcc gcagcagctc gacgagttcc acgaggcgca
     3121 ggagcaggtg gggttcgcag accgcatcct gctttcgaag accgatttaa cagaggaagc
     3181 agagacggaa ctgctgatca agagattgaa aaaaatgaat cctcgcgcgc cgatcaagaa
     3241 ggtgagcttc ggcgacgcgc cgctcgacga ggtgctcgat atccgtggct tcaacctcaa
     3301 cgcgatcctc gagctcgacc ccgagttcct caccgacgtg cagcacgagc accacgacca
     3361 ggtcgagtcg ttcgtgttcc gcgcgaagaa gccgttcgac ggcaccaagc tcgagcagtt
     3421 cctctcgggc atgatccagg tgtacggccc cgacctgctg cgctacaagg gcgtgctgtg
     3481 gatgaagggc aatccgcgcc gcgtcgtgtt ccagggcgtg cacatgatga tgggcggcga
     3541 tctgggcaag ccctggacca gggccgacaa gaaggaatcg ctgctggtgt tcatcggcaa
     3601 gaagctgccg aaggacctgt tcatcgccgg cctcgaggaa tgtctggcaa aatgatgcgg
     3661 tcatgagcct gcggcgctac gccgtgttac gcatcgtctg cgccttcctg ctgcttcttg
     3721 cgcagcaggg cgctctcacc catgacatct ggcattcggc aaaggctgcc tcgcacgccg
     3781 acgtctccat cgccggcgat gcgaaggacg tcaagaagaa tcggctgtgc gagctgcatg
     3841 ccgccctcgg ctccgtgctt ggcgcgctcg gcggcaacgc acctgccttg gctgtcgccg
     3901 atacgaacga gatcggcttc aagaccgcgg atgcgtccgc gataatcgtt cctccgctcc
     3961 ctcccgcttc ccggggtccg ccgacgctcc tctgaaatgt ttcgagcgcg cgccacggcg
     4021 gcgcgcatta cgaactttcg agggagcggt tcatgtatcg caacatgtgg tgcgccgcgg
     4081 cgcttgccgc ggcgatgctt ccggcgcagg cgcaggacgt gaagcaactc gagcggcgcg
     4141 tacaggagct cgagcagcgc gtcgccgaaa atacgccgaa gcagagctct aacgcgttca
     4201 atcccgacat ttccgtgatc ctgcagggca cggcggcacg cagctcgcag gacccggcga
     4261 acttccagat caccggcttc gcgccggccg ggggcgaagt cgccccggcg cgccgcggct
     4321 tcagcctcgg cgagtcggag ctcgtcttcg cctcgaacat cgatccctac ttccgcggtc
     4381 aactggtggc tgcgctggct cccgacgatg cagtcgaggt cgaagaggcg ttcttccaga
     4441 ccttgcggct cggccgcggc ttgaccgtga agggcgggcg attcctctcg ggcatcggct
     4501 accagaaccc gatccaccag cacgcctggg atttccagga tgcgccgctc gcttacaagg
     4561 cgtttctcgg cggacgcctg aacgacgacg gcgtgcagct gaagtgggtg gcgccgagcg
     4621 agctgctggt cgagctcggc agcgagctcg gccgcggccg gagcttcccg gccaccgatc
     4681 cgaacaagaa cggcgcagcg gcctggaccc tgttcggcca tgccggcggc gacttcggcg
     4741 cgagcgccgc ctggcgcgca ggactctcgc accttcgcac ctcgccgcgc gagcgcacgt
     4801 tcgacgacgt ggatacatcg ggcaatccgg tgacgcagtc cttcaccggg tcgagccggc
     4861 tatggatcgc ggacttcgtg ctgaagtggg cgcctgacgg caattcgacg gtcaccaact
     4921 tcaagctgca gggcgagttt ttccgccgca gcgagtcggg cgagctgacc ttcaacaacg
     4981 ccatgaccgg cgactatgcg agccggcagt ccggctggta cctgcagggc gtctaccagt
     5041 tcatgccgca atggcgcgcg ggataccgct acgaccggct gagccacggc agcgtatcga
     5101 acgggctcgg cttgacggcg ggggattcgc ctctgctgct caccgagcat aacccgacgc
     5161 gccacacgct gatgctggac tggagcgcga ccgagttcag ccgcatccgg ctgcagctcg
     5221 cctccgacaa gtcgcgcgcc ggcgtcaccg acaaccaggt gctgctgcag tacatctaca
     5281 gcctcggcac gcacggcgcg cacacgtttt agggatcaca tcatgcgatt tcttctgacg
     5341 tttcttttat ttgtctcctc atggccggca tgcgctgcgc tcaatattct ggcctgcacc
     5401 ccggaatggg gtgcgctcgc caaggagctc ggcggcgaaa aggtaagcgt gtacgtcgct
     5461 tcgaccgcgc tgcaggatcc gcatcgcatc gaggcgcggc caagcctcat cgcgagggcg
     5521 cgcagcgcgg acctgatggt gtgcaccggt gcggagctcg agatcggctg ggtgcctctc
     5581 ctgcagacgc agtccgggaa cgagaaaatc caggtcggcg caccgggctt tttcgaagcg
     5641 gcgagcgtcg tgccgctgct ggaaaggcca aatcggctcg accgctcgat gggcgacgtg
     5701 catcctggcg gcaatccgca catccacctg gatccgcgca acgtggcgaa ggtggcggct
     5761 gcccttggcg agcgcatggc ggcgctggac ccgcgcgagg cggcgtatta cagcgaccgg
     5821 tccaactcct ttctggagcg gtgggaacaa gcgcggatcc gatgggagaa agaggcgatc
     5881 cctcttaagg ggttgccgct ggtcgtgtat cacaagaaca tgacctacat gatccactgg
     5941 ctcggcatgc gcgaagtcgg caatctcgag ccgaagccgg ggctgccgcc gacgaccgcg
     6001 catctgagcg aattgctggc gaatctccag cggcaaccgg cgaaggcggt cgtgcgctcc
     6061 gcgtataacg atccgcgacc gggtgaatgg ctcgccgagc gcgcgaagat tccgtcggtc
     6121 acggtcgcct tcacggtcgg cggaacgcct gaggcaaagg atctgttcgc tctctacgac
     6181 gatacgctgg caaggctgct ggcggtcgcg aaatgagcgc ggcgacgctc gacctcagca
     6241 tcgtgtggcc ggcgctcgtc gccgggctgc tcgtcaccgc cacccacgtg ccactcggca
     6301 tccaggtgct gaagcggggc atcgtgttca tcgatctggc ggtggcgcag atcgcgggcc
     6361 tgggcgtggt cttcgcggac tggctcggct tcgagccgca cggcgccgcc gtccaggccg
     6421 cggcattgtc cgccgcgctc gggggcgcgc tgttgctcaa ctggaccgac aagcgatggc
     6481 ccgaggtgca ggaggcggtg atcggggtca ctttcatcgt cgccgccaat gcggcaatcc
     6541 tgctgctcgc cgccaatccg cgcggcggcg agcatctcaa agacttgctg atcggccaga
     6601 tcctctgggt gagacccgtc gagctgcccg tcgttgcggc cgtttccgcc gctatcgtcg
     6661 caatatggtt cggcgccggc cagcgcattg gccgcatcgg cttctatctg ttgttcgcgt
     6721 gcgcggtcac cgcttcggtg cagctcgtgg gcatctacct ggtcttcacc accctcatcg
     6781 cgccggcgct tgccactcgt catctggcac gccaccgcct tgcttcgtgc tatgtgctcg
     6841 gcgcgctcgg ttatgccgtg ggcctcgggc tgtcgctggt caccgacctg ccgcccgggc
     6901 cgctcatcgt ctgcgccatg actgcgctcg gcatcgcgct cgccatcgct acatcgcagc
     6961 gcgccgctac gtagccggtg cgatcagtgg gcgtgtcgcg taccggcgta gccgcgtagt
     7021 tcgtcgtagc gcctcacctg ctcatcggtg agcagcgccc gcgtgcgacg atgcgtttcg
     7081 aggtgcgaca ggcggtagtc gcccgcgagc tgcgctgcag tccgaaccgc gcgcgcaagc
     7141 gcagcctcgt ccacccggcc cgagcgaaaa agctgctcga gcccgtattc ggagtcaacc
     7201 agctttgcgc caatcgctcg agcctccgca tgatgcgcgc tcgcgagcgc tttggcggcc
     7261 gcgcgctgct cggcgctcaa gccaagcttg tcggcgaact cgagcgcgtg ccctggcccg
     7321 ggatagtggt tgagctcggc gctcttcgca tagcccatcc ccgcgcccga aagatattgc
     7381 ttcacctcgt ccgccgagag cgccttgatc tcgcgctcgt gctggcctgc gtaaggctga
     7441 tgctgcgcgt gcgcggcccc tgccagcaac catgctgcga tcatgacttg aggtttcatc
     7501 ttgcttctcc ttgacttcac cacgtcctga agggaatggc gagcgcgccg gacagcgtcg
     7561 tcgacaccca ggcgtcgcgg tagctatcga gtcccagccg catgccggtc tggccgcccg
     7621 ccggagacag cgtataggtg ccgaacagcc gatcccatgc cgagatgcag aagccgtaat
     7681 tgctgttgcg ctccgcttca atgaccgagt ggtgaatgcg gtgcatgtcc ggcgtcacca
     7741 ggacaaagcg aagggcccga tcaatgatct cgggcaagcg cacgttgccg tgattgaaga
     7801 gcgaactcac gccaagcagc atttcgaaca ccagcaccgc caccgcgggg gtgccgagcg
     7861 cgactatcac tgcgtacttc aacagcatcg agaggataat ctcgatcgga tggaaacgaa
     7921 ttccggtcgt ggcgtcgaca tccgggtcgg catggtgcac cgcgtggatc ctcatcagca
     7981 cgggcgtgag atgaaatgcg acgtgttgca gatagatgaa aagatccagc gccaggaccg
     8041 cgatcacaac cgccagccag gactcgatct ccagctggtt gaacaggccc cagccgcgcg
     8101 ccgcggcaag ctcggcgatg ccgatcgccg tcgtcggcag tgcgacgcgg accaacagga
     8161 cattgagcag cgcgacgccg agattggtgc gccagcgtcg cgccttggat tcgcgcggtg
     8221 agcgccgcgg cgcgaagacc tcccaggcag ccatccctgt caacgccagg gccatcgcgg
     8281 tgacgcgtat cggcgcttcg tgctcgagga ggaaggcatt gtccattttg ggctgccgcg
     8341 gctaccatcg cccggccgct tctccgcaac tgttcaactg attagttgaa tagtagaata
     8401 cagcccatgc acaagtcaag cgacccggaa aagggccggc tctacgaggc gatcggccgc
     8461 gtcgcggcgg cgctcggcag cgccgggcgc ctgcagatcc tcgagctcgt cgcccagggc
     8521 gagcgcagcg tcgacgcgct cgccgccatg accgggctct cggtcgccaa tacttccaag
     8581 cacttgcagg ccttgcgcca ggccggcctg gtcagcgcgc ggaagcaggg gctgcgcgtg
     8641 tactacgtgc tcgccggcga cgatgtcacc gctctggtcg cggcgctcag ggcggtggct
     8701 gaacaccgcg ccgcggacgt cgagcggctg gtgcgcacct ggctagcgca ccgcgatgag
     8761 ctcgagcccg tgcccgcggc cgaactactg caacgagcca agaaggggct ggtcacggtc
     8821 ctcgatgtcc ggccggcgga ggaatacgcg gccgggcacg tgcccggggc gatcaacgtg
     8881 ccggtcgaca agctggagag ctatctctcg aagctgccaa agcgcaagga agtcgtcgct
     8941 tattgccgcg gtccctactg cctgatgtcg ttcgacgcgg tcgagaaact gagaaagcgc
     9001 ggctggcgcg cgcggcgcct gcaggacggc ttcccggagt ggcgcgccgc cggattgccg
     9061 gtcgagactt gagctcaagc cgcgcgcgta cttctggcga gatacagggc gagcggcggg
     9121 accgcaagcc attgcagcag aggtgataac cctagctcga cacctgccac cctgagcgtc
     9181 ggcatcagct gggaataggc ccaagctgga cgcagtactg tgttcgtcca ctcgctgaat
     9241 cccgtgtagg caacgccgaa gacggcaagc aggaaagcca cggcctgcca gttccacgtt
     9301 tccataccgc ctgcgcgcgt agcgagcagc gcgagcgcga gcgagccgag gccagtcagc
     9361 acgtcgccgg ccgtgcagtg cgcgagcgca aaggcgatgt accccgccgc cgcctcgctc
     9421 catatcgtat agagcggaag ctgcaggatc tcccaggcga gactgagagc cgcgagccaa
     9481 ggcaggtagc cccgcaggat cagcgagcgc gctgcgctgt cacggtacca gcctgcggcg
     9541 gtcatttcgg aattagcgct ttctggcgtt ccgagtcatt tccgctcgca aggggagaac
     9601 ctcctgcgtc caacggctct tgatgtacgc gagcacggcc cagatttcgt cctccgagag
     9661 cttccccccg aaggctggca tgtcgctctc gtagcccgca ggcgcataag gcggcacgag
     9721 ccctctcttc gtgatgccga agagcaactg gtcggagtgg tgccaagtgt ggccgctgtc
     9781 gtcgtgcggc ggcgcaggca gacggccgtt cggaagtcgg atgcgccagt tcggctggcc
     9841 ttcgagcctt gcgccgtggc atgccgcgca gtgctgcgca tagaccttcg cgcccagcgc
     9901 gactttcgcc gcattgcgcg gatctgcgcg cgcatcgtcg ccggggcttg gcgattcagt
     9961 gcacgcggcg agggtgagcg ccagcgcgat acgcctcagg ggcacgatgc cttgacgaat
    10021 tcgctgatct ccttcggtga ggcgaaaacg atcgacgcgc ggtccccctg catgccggcg
    10081 ccgacgacga tcggcttacc aggttccgga ccgagcttcg tcgagtcgga cttgaagcgc
    10141 aggttgaact cccagatttt ttgcctcttg ccgtcggcag tttccaccgt gtaggcatcg
    10201 ccgcaataac ggatggagcg aacctgtcct tcaggcgggg ctttacgcag atcgagcttc
    10261 gtgccgcgca ttcccatcat tcctccacct gtttgcggcg ctttgttctc ggcaaccgcc
    10321 ttgaggtagg cggccatatc ctcgcggtct ttgcgctctt tcaagccggg aaacgtcatg
    10381 ctggtccccg ggagaaactt ctcgggattg gcgagccact tgtcgagtgc ggcttcgttc
    10441 caggtgatgc cgagccgctt catcggctcc gaatagcgtt ggaatccgtc gaccgttccc
    10501 gccttgcggt tccagatgtg cgcgaggctt gggccggtca tgtgctcgcc gggttcgacc
    10561 gaatggcagg ccatgcattg cccgaagagc tgcgcgccgc gtttcgcgtc gcccgcgccg
    10621 agcgccgcgc tgccgggcaa aagagccagc agagcgagca tcctgatgag accttgcact
    10681 ttgttcaact ggggtgagca catagtcaaa tcctttcaac cgatgcgacc cagcagcgga
    10741 aagcgctcaa gcagccagga gctgaacgcc gtgagcttgc cggtcatcat c
//
LOCUS       DASPXW010000346         5046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_606300, whole genome shotgun sequence.
ACCESSION   DASPXW010000346 DASPXW010000000
VERSION     DASPXW010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5046
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606300"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VEL04_07855"
     CDS             complement(<1..253)
                     /locus_tag="VEL04_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0758 domain-containing protein"
                     /protein_id="HYT48319.1"
                     /translation="MAISDWPAAERPRERLLEQGAASLSNAELLAVLLRTGIRGKSAV
                     DLARQLLDEFHGLAPLLEAGAKLARVKGLGPAKSAQFTAA"
     gene            309..1502
                     /gene="coaBC"
                     /locus_tag="VEL04_07860"
     CDS             309..1502
                     /gene="coaBC"
                     /locus_tag="VEL04_07860"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HYT48320.1"
                     /translation="MTLKGKRILLGLTGGIAAYKAAELTRLLVKAGADVRVAMTGAAT
                     RFITPVTLQALSGQNVWTDLWDARVADNMGHIELSRDRELILVAPASADFLAKLAHGL
                     ADDLLSTLCLARRCPLMVAPAMNVEMWENVATARNAETLRADGVVISGPASGGQACGE
                     MGMGRMTEPADILADVEFFFQPKRLQGKRVVVTAGPTEEPVDPVRVLTNTSSGKMGYA
                     VARAAQEAGAEVTLISGPVSLPTPAGVARVDVRTAREMFDAVKKDAKSADVFISVAAV
                     ADYRVKHPSGQKIKKANGRSLSLELEENPDILAWVAALSKGPFCVGFAAESEQLGEHA
                     KEKRARKNIPLIAANLAHEALGADENSITLYDDRGEHPLGRGPKLQLARKLVEHVAGM
                     LPRKK"
     gene            1499..1945
                     /gene="dut"
                     /locus_tag="VEL04_07865"
     CDS             1499..1945
                     /gene="dut"
                     /locus_tag="VEL04_07865"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HYT48321.1"
                     /translation="MKKLEVKILDERIRGMLPRYATAGAAGLDLRACIEAPLTLQPGD
                     SQLVHSGIAIHVADPGYAAVVLPRSGLGSKHGIVLGNLVGLIDSDYQGEVLVSVWNRG
                     HASFTIQPLDRIAQLLVVPVTQVDLEVVEEFAESSRGAGGFGSTGR"
     gene            complement(1975..3225)
                     /locus_tag="VEL04_07870"
     CDS             complement(1975..3225)
                     /locus_tag="VEL04_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HYT48322.1"
                     /translation="MKRRDFLKIAGAVSLAGCAATTPSKARVVVIGGGYGGATAAKYI
                     RMWDPAIDVVMVERESTFISCPLSNLVLGGSKSLDDLTRSRDGLRKHGVQIVNDDATM
                     VDTTKKVVRLARGGDLAYQRLIVSPGIDFLFNEVEGYEAAMNSGRVLHAWKAGAQTVE
                     LRKQLVGMKDGGVYVLAIPTAPYRCPPGPYERVCQVAHYFRQAKPRSKILLLDANPDV
                     TSKGPLFKRAWEELYTGMVEYRPNQKAIGVDAAAMAVKLEFENVSGDVLNVVPPHRAG
                     DIAVKTGLITHNNRWCDVDWRTLESKAAKGVHVLGDATLSASGMPKSGHMANSQAKVC
                     AAAVIALLNGREPSVNPVMNNTCYSFVSDREVIHVASVHQWDAAQGTIVPVKGAGGVS
                     VARSGQEAVYGWSWAQNIWADMLA"
     gene            complement(3233..3526)
                     /locus_tag="VEL04_07875"
     CDS             complement(3233..3526)
                     /locus_tag="VEL04_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HYT48323.1"
                     /translation="MRALLLIAAAAPLVALAQDDGAARSLAATCANCHGTDGRSATQE
                     VMPLAGLPKSFLVDQMKAFKEGKRPATIMHQLAKGYTDQQIELIADYLAAQKK"
     gene            complement(3586..>5046)
                     /locus_tag="VEL04_07880"
     CDS             complement(3586..>5046)
                     /locus_tag="VEL04_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010395947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYT48324.1"
                     /translation="KAVLKQLADNPKSILFIDEIHTIIGAGAASGGTLDASNLLKPAL
                     QSGQLKCLGATTYTEYRGVFEKDHALSRRFQKIDVVEPSVQETVEILKGLKTRFEAHH
                     SVKYTANALTTAAELSARYINDRHLPDKAIDVIDEAGAAQRIAPKAKQKKIIGKSEIE
                     EIIAKIARIPPRSVSTDDRSALANLDRDLKAVVFGQDRAIDALASAIKMARSGLGNPQ
                     KPIGCFLFSGPTGVGKTEVARQLAYCMGIELIRFDMSEYMERHAVSRLIGAPPGYVGF
                     EQGGLLTEQITKKPYSVLLLDEIEKAHPDIFNILLQVMDHGTLTDNNGRKADFRNVCI
                     VMTTNAGAESLAKNSMGFTMNKQSGDEMEAIKRMFTPEFRNRLDATISFKALDHDIIL
                     RVVDKFLMQLEEQLQEKKVEVSFTRALKEHLAKKGFDPLMGARPMARLIQDTIRRALA
                     DELLFGRLQHGGKVSIDLGEEEKIKLDFEEEAAPVA"
BASE COUNT          799 a         1634 c         1670 g          943 t
ORIGIN      
        1 ttgcggcggt gaactgcgcg cttttggccg ggccaagccc cttgacgcgc gcgagcttcg
       61 cgcccgcttc gagcaagggc gcaaggccgt gaaattcgtc gagcagctgg cgggccagat
      121 cgacggcgct tttgccgcga atgcccgtgc gcagcagcac cgcgagcagc tcagcgttgc
      181 tcaagctggc agcgccctgc tcgagcagcc gctcgcgcgg ccgttcggcc gccggccagt
      241 ccgaaatcgc catgcccggg cctttccctt agaatcgtca tcctcttaac ggctccccgc
      301 ccctggcgat gacattgaag ggaaagagaa ttctgctcgg cctgacgggc ggcatcgccg
      361 cttacaaggc ggctgagctg acccggctgc tggtcaaggc cggcgcagac gtgcgtgtcg
      421 ccatgaccgg ggccgcgacc cggttcatta cgcccgttac tctgcaggcg ctctccgggc
      481 agaacgtctg gaccgatctg tgggatgcac gcgtcgcgga caacatgggc cacatcgagt
      541 tgtctcgcga ccgcgagctg atcttggtag cgcctgcgtc ggctgacttc ctcgccaagc
      601 tggcgcacgg ccttgccgac gatctgcttt ccacgctttg cctggcgcgg cgctgcccgt
      661 tgatggtggc gccggccatg aacgtcgaga tgtgggaaaa cgtggcgaca gcgaggaatg
      721 ccgagacgct gcgcgcggac ggcgtggtga tctccgggcc cgcttcgggc ggccaggcct
      781 gcggcgaaat gggcatgggt cgcatgaccg agcccgccga catcctcgcc gacgtggagt
      841 ttttttttca gcccaaacga ctccagggca agcgcgtcgt cgtaaccgca ggtccgactg
      901 aggagccggt cgacccggtc cgagtcctga ccaataccag ctcgggaaag atgggctacg
      961 cggtggcgcg cgccgcgcag gaggcgggcg ccgaagtcac gctgatcagc ggcccggtct
     1021 cgctgccgac gccggcgggc gtggcgcgcg tggacgtgcg cacggctcgg gaaatgttcg
     1081 atgcggtgaa gaaggacgca aagtcggccg acgttttcat ctcggtggct gcggtcgccg
     1141 attaccgcgt caaacatccg tccggccaga aaatcaagaa ggcgaacggc cggagccttt
     1201 cgctcgaact cgaagagaac ccggacatcc tcgcctgggt ggcggcgttg tcgaaagggc
     1261 cgttctgcgt cggcttcgcc gcggaaagcg agcagctggg cgagcacgca aaggaaaagc
     1321 gtgcgcgcaa gaacattccg ctgatcgccg ctaatctcgc gcacgaggcg ctaggtgccg
     1381 acgaaaactc gatcacgctc tacgatgatc gcggcgagca tccgctaggt cgtggtccga
     1441 aactgcagct ggcgcgcaag ctggtcgagc acgtcgccgg catgctgccg cggaagaaat
     1501 gaaaaagctg gaagtgaaga tcctcgacga gaggatccgc ggcatgctgc cgcgctacgc
     1561 taccgcgggc gcggcggggc tcgacctgcg ggcctgcatc gaggctccgc tcacgctgca
     1621 accgggcgac agccagctgg ttcactccgg catcgcgatc catgtcgccg atcccggcta
     1681 cgccgcagtc gtcctgccgc gctcggggct ggggtcgaag cacggcatcg tgctcggcaa
     1741 cctggtcggc ctgatcgact ccgactacca gggagaagtg ctggtttcgg tctggaaccg
     1801 cggccacgca tctttcacca tccagccgct cgatcgcatc gcgcagctcc tggtggtgcc
     1861 ggtaacgcag gtcgacctcg aggtggtcga ggagttcgcc gaaagcagcc gcggagccgg
     1921 gggcttcggc tccaccggac gctaaggggg tcaggtgatt cacacccccg cttcctacgc
     1981 aagcatgtcc gcccagatgt tctgggccca agaccagccg taaaccgctt cctgcccgga
     2041 gcgcgcaacg gagactcctc ccgcgccttt caccggaacg atcgtgccct gcgccgcgtc
     2101 ccattggtgc accgaggcga catggatcac ttccctgtcg gagacgaagc tgtaacaggt
     2161 attgttcatc accggattga cgctgggctc gcgaccgttc aacagggcga tcacagcggc
     2221 cgcgcagact tttgcctgcg aattcgccat gtggccggat ttgggcatgc cgctcgctga
     2281 aagcgtcgcg tcgccgagca cgtgcacgcc tttcgccgcc ttggactcga gcgtgcgcca
     2341 gtcgacgtcg caccagcggt tgttgtgggt gatgagaccg gtcttgaccg cgatgtcgcc
     2401 tgcgcggtgc ggcggaacga cgttcagcac gtcgccggac acgttctcga actcgagctt
     2461 caccgccatc gccgcggcat ccacgccgat cgccttctga ttgggccgat attcgaccat
     2521 gcccgtgtag agctcctccc aggcgcgctt gaagagcggc cccttcgacg tcacgtccgg
     2581 attggcgtcg agcagcagga tcttggagcg cggcttagcc tgccggaagt aatgcgcgac
     2641 ctggcagact ctttcgtacg ggcccggcgg gcagcggtag ggcgcagttg gaatcgcgag
     2701 cacgtagacg ccgccgtcct tcatgccgac cagctgtttc ctcagctcca cggtctgcgc
     2761 accggctttc caggcgtgca gcacgcggcc ggaattcatc gccgcctcgt agccctcgac
     2821 ttcattgaac aggaagtcga tgccgggcga gacgatgagg cgctggtacg cgagatcgcc
     2881 gccacgcgcg aggcgcacga ctttcttcgt cgtgtcgacc atggtggcgt cgtcgttcac
     2941 gatctgcacg ccgtgcttgc gcagcccgtc gcggctgcgc gtcaggtcgt cgagcgactt
     3001 gctgcctccc agcaccaggt tcgagagcgg gcacgagatg aatgtgcttt cccgctccac
     3061 catcaccacg tcgatcgccg ggtcccacat ccggatgtac tttgctgcgg tcgccccgcc
     3121 atagccgcct ccgatcacga ccacgcgtgc tttggacggg gtcgtcgcgg cgcatccggc
     3181 gagggaaacc gctccggcga tcttcagaaa gtcgcgtcgt ttcatggctg cctcacttct
     3241 tctgcgcggc gagatagtcg gcgatcagct cgatctgctg gtcggtatag cccttggcga
     3301 gctggtgcat gatggtggcc ggccgcttgc cttccttgaa tgccttcatc tggtcgacca
     3361 ggaagctctt cggcaggccg gcaagcggca tgacttcctg ggttgcactg cggccgtccg
     3421 taccatggca gttggcacag gtggcggcca ggctgcgtgc cgcgccgtcg tcctgggcaa
     3481 gcgcgacgag cggcgccgct gcggcgatga ggagcagggc tctcatacga tctccatgga
     3541 aaaaggccgc aagcagcggc ctttgcttgt tcccctagcg ccgaatcagg cgaccggcgc
     3601 cgcctcttcc tcgaagtcga gcttgatctt ctcttcttcg ccgaggtcga tgctgacctt
     3661 gccgccgtgc tgcaggcggc cgaacagcag ctcgtcggcg agcgcgcgcc ggatggtgtc
     3721 ctggatgagg cgcgccatcg gccgggcgcc catcagcggg tcgaagcctt tcttcgccag
     3781 gtgctccttg agcgcccggg tgaagctcac ctcgaccttc ttctcctgca gctgctcttc
     3841 cagctgcatc aggaacttgt cgacgacgcg caggatgatg tcgtggtcga gcgccttgaa
     3901 cgagatggtg gcgtcgagcc ggttcctgaa ctcgggcgtg aacatgcgct tgatggcctc
     3961 catctcgtcg ccgctctgct tgttcatggt gaagcccatc gagttcttcg ccagcgactc
     4021 ggcgcccgcg ttggtggtca tgacgatgca cacgttgcgg aagtcggcct tcctgccgtt
     4081 gttgtccgtc agcgtgccgt ggtccatcac ctgcaggagg atgttgaaga tgtcggggtg
     4141 cgccttctcg atctcgtcga gcagcagcac cgaatacggc ttcttcgtga tctgctcggt
     4201 gagcaggccg ccctgctcga accccacata ccccggaggc gcgccgatca ggcgcgacac
     4261 cgcgtgccgc tccatgtact ccgacatgtc gaagcggatc agctcgatcc ccatgcagta
     4321 ggcgagttgg cgcgcgacct cggtcttgcc gactccggtc ggtcccgaga acaggaagca
     4381 gccgatcggc ttctgcgggt tgcccaggcc cgagcgcgcc atcttgatgg cggaagcgag
     4441 cgcgtcgata gcgcgatcct gcccgaacac caccgccttg aggtcgcggt ccaggttggc
     4501 gagcgcgctg cggtcgtcgg tcgagacgct gcgcggcggg atgcgcgcga tcttggcgat
     4561 gatctcctcg atctcggact tgccgatgat cttcttctgc ttcgccttgg gcgcgatgcg
     4621 ctgcgccgcg ccggcctcgt cgatgacgtc gatcgccttg tcgggcaggt ggcggtcgtt
     4681 gatgtagcgc gcagagagct cggcggccgt ggtgagcgcg ttggcggtgt acttgacgct
     4741 gtggtgcgct tcgaagcgcg tcttcagacc cttcaggatc tcgacggttt cctggaccga
     4801 aggctcgacg acgtcgattt tctggaagcg gcgcgacagc gcgtggtctt tttcgaacac
     4861 gccgcggtac tcggtgtagg tggtcgcgcc gagacacttg agctggccgc tctgcagcgc
     4921 cggcttgagc aggttggatg cgtcgagcgt gccgccggaa gccgcgccgg cgccgatgat
     4981 cgtgtggatc tcgtcgatga acagaatcga cttcgggttg tcggcgagct gcttcagcac
     5041 ggcctt
//
LOCUS       DASPXW010000347         4410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1239363, whole genome shotgun sequence.
ACCESSION   DASPXW010000347 DASPXW010000000
VERSION     DASPXW010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4410
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1239363"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..489
                     /locus_tag="VEL04_07885"
     CDS             4..489
                     /locus_tag="VEL04_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010811673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HYT48325.1"
                     /translation="MRHAQLGLTVNGESHEVSFAPYKTLLELLREDLGLTGTKHGCEL
                     GECGACAVLVDGVPQLSCLVLALECEGRSVETVEGMARGAELHPLQAAFADLGAAQCG
                     YCTPGILMTAKALLSHEKNPSRERIKEAISGNLCRCTGYQQIFEAIEEAARRLALESP
                     R"
     gene            473..2911
                     /locus_tag="VEL04_07890"
     CDS             473..2911
                     /locus_tag="VEL04_07890"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HYT48326.1"
                     /translation="MKVLGKPRRRVDGRAKVTGQTRFADDIVLPRMLHMKLLRSPHAH
                     ALIEKIDIEKAKSHPGVHLVLTGKDFPVAFGILPVTQDEYPLAPRHVRYVGDPVAAVI
                     AKDEQTATEALDLIEVRYKVLDTISNPEEALAKAEPRIHAYSELGNIHRLQAFEFAAG
                     KDAVEDTLRKSDHVFEDLFFYEGNTHLPIEQHAAVASLDGEGKLTLWSSTQVPHYVHR
                     SLARVLQKPATHIRVIACPNGGGFGGKCDVCNHEMVVAKAAVMLGRPVKICLNREEVF
                     YLHRGRHPVLMKFRTGVSREGRLTGMHLQTLVDGGGYGSYGVASTFYTGALQTVTYEL
                     PRYKFEAARVFTNKPPCGPKRGHGTPQPRFGQEVQLDKIAEKLGVDPAELRLQNVLKA
                     NSLTANWLKIGSIGLSECIRQVVERSGWKGRFKKMGFGKGLGLACGSYLCGAGLPIYW
                     NRMPQTGVHLLLDRSGQVTVFCGATEIGQGSDDVLVAIVAEVLGIDPFDVRCVTGDTG
                     MTPVDLGSYSSRVTVMMGNAAIQAAEKIKQQIAIAASEQLGVAPDRLTFADGRVFFES
                     KSLTFVEAAGFAEEKFGTLGSVGSYKPPRSPGRYKGAGVGPSPAYSYTACVVEVDVDP
                     ATGWITVPKVWIAHDIGRTINPVLARGQVEGSVYMGLSEALMEEQIFRRLPPQLSSAL
                     VHKIPSLLEYKSLTSLDMPEVDTVLIEDPDPNCPFGAKEAGQGPLLPVMPAVANAIYD
                     AVGVRIDELPITPDKVLRALEMKASGKEARVGPSHFPAVPYPEPVIVPPPWEGGDGNA
                     LKDFEKNKMKIAAP"
     gene            2908..3888
                     /locus_tag="VEL04_07895"
     CDS             2908..3888
                     /locus_tag="VEL04_07895"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HYT48327.1"
                     /translation="MMRLPWFEYRAPKSVAEAARILAAEGPAAVLIAGGTDLLPNMKR
                     RQMAPKMLISLKRITQLRQNGAILGAGLTLSEVVKKKELPAALRQAAGQVATVHLRNM
                     GTLGGNLCLDTRCNYYNQNYEWRKAIDFCLKKDGDICWVATASKRCVAMSSTDCAPAL
                     MALGARVKLVSSKNERELALEELYNNDGIDYLRRRPDEILTEIRIPESTASCYWKLRR
                     RGAFDFPVLGVAAALKDGNVRIALGAVASRPFLVDKASEFLKGKQLTDEVIEEAGRIV
                     ASRAKPMDNADLDLYWRKGVAADFVGYALRELRGDDMRATRLRIARYAVT"
     gene            3958..>4410
                     /locus_tag="VEL04_07900"
     CDS             3958..>4410
                     /locus_tag="VEL04_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48328.1"
                     /translation="MRKSAAPASATVRALLCCLFTVALDASGAQPMKPEEVPAPLKPW
                     IPWALHGTAESPCPLMAGKPDERACAWPGLLELDMHATGGKFRQAIRVYGPPAAPLWV
                     AVPGGAGRWPQNVTSNGKVEAVIGRDGVPGVYLAPGEHRLAGQFAWSAL"
BASE COUNT          847 a         1411 c         1435 g          717 t
ORIGIN      
        1 aaaatgcgcc acgcgcagct cggcctgacg gtcaacggcg agagccacga agtctccttc
       61 gcgccctaca agacgctgct cgagctgctg cgcgaagacc tcggcctcac cggcaccaag
      121 cacggctgcg agctcggcga gtgcggcgcc tgcgcggtgc tggtcgacgg cgtgccgcag
      181 ctttcctgcc tggtgctcgc gctcgagtgc gagggccgct cggtggagac cgtcgaaggc
      241 atggcgcgcg gcgccgagct gcacccgctg caggccgcgt tcgccgacct cggtgccgcg
      301 cagtgcggct actgcacgcc tggaattctc atgacggcaa aggctttgct gtcacacgaa
      361 aaaaatcctt ccagagaaag aatcaaggag gcgatatcag gcaacctttg ccgctgcacc
      421 gggtatcagc agatcttcga ggcaatcgaa gaagctgcgc gaaggctggc gcttgaaagt
      481 cctcggtaag cctcgtcggc gcgtcgacgg ccgcgccaag gtcaccggcc agacgcggtt
      541 cgccgacgac atcgtcctgc cgcgcatgct gcacatgaag ctgctgcgct cgccgcatgc
      601 ccatgcactt atagaaaaaa ttgacataga aaaggcaaaa agccatccgg gtgttcacct
      661 ggtcctcacc ggcaaggatt tcccggtcgc gttcggcatc ctgccggtca cccaggacga
      721 atacccgctc gcgccccggc acgtgcgcta cgtcggcgat ccggtggcgg cagtgatcgc
      781 caaggacgag caaaccgcca ctgaggcgct cgacctgatc gaggttcggt acaaggtcct
      841 cgacaccatc agtaacccgg aagaagccct ggccaaggca gaaccgagaa tccacgccta
      901 cagcgagctc ggcaacatcc accgcctgca ggccttcgag ttcgcagccg ggaaagatgc
      961 ggtggaagat acgctgcgca aatcggacca tgtcttcgaa gacctgttct tctacgaggg
     1021 caacacgcac ctgccgatcg agcagcacgc cgccgtggcc tcgctcgacg gcgagggcaa
     1081 gctcacgctc tggtcctcga cccaggtgcc gcattacgtg caccgctcgc tcgcgcgcgt
     1141 gctgcagaag ccggccacgc acatccgcgt catcgcctgc ccgaacggcg gcggcttcgg
     1201 cggcaagtgc gacgtctgca accacgagat ggtggtcgcc aaggcggccg tgatgctcgg
     1261 gcggccggtg aagatctgcc tcaatcgcga ggaggtcttc tacctgcacc gcggccgcca
     1321 ccccgtgctg atgaagttca ggaccggagt aagcagggaa ggcaggctca ccggcatgca
     1381 cctgcagacg ctggtggacg gcggcggcta cggctcgtac ggcgtcgcga gcactttcta
     1441 caccggggcg ctgcagaccg tgacctacga gctgccgcgc tacaagttcg aggccgcgcg
     1501 cgtctttacc aacaagccgc cctgcggccc gaagcgcggg cacggcacgc cacagccgcg
     1561 cttcggccag gaagtgcagc tcgacaagat cgccgagaag ctcggcgtgg atcctgccga
     1621 attgcgttta caaaatgttc tcaaggcaaa ttcgctcact gcgaactggc tgaaaatcgg
     1681 cagcatcggc ctctccgagt gcatcaggca ggtcgtcgag cgctccggct ggaaaggccg
     1741 tttcaaaaaa atggggtttg gaaaaggcct tggcctggcc tgcggctcct acctctgtgg
     1801 cgcagggctg ccgatctact ggaacagaat gccgcagacg ggcgtgcacc tcctgctcga
     1861 ccgcagcggc caggtgacgg tgttctgcgg cgccaccgag atcgggcagg gctcggacga
     1921 cgtgctggtg gcgatcgtcg ccgaagtgct cggcatcgat ccgttcgacg tgcgctgcgt
     1981 gacaggcgat accggcatga cgccggtcga cctcggctcg tattccagcc gggtgacggt
     2041 gatgatgggc aatgccgcaa tccaggctgc cgaaaagata aagcagcaga tcgcgatcgc
     2101 ggcctccgag cagctcggcg tcgcgcccga ccggctgact tttgcagatg gaagagtttt
     2161 ctttgaatct aaatccctga cattcgtgga ggcggcaggg tttgccgagg agaaattcgg
     2221 cacgctcggc agcgtcggct cgtacaagcc gccgcgctcg cccggacgtt acaagggcgc
     2281 gggcgtcggc ccctcgccgg cctattcgta cacggcttgc gtggtcgagg tcgacgtcga
     2341 tccggcgacg ggctggatca cggtgcccaa ggtctggatc gcgcatgaca tcggcaggac
     2401 catcaacccg gtgctcgccc gcggccaggt cgagggcagc gtctacatgg gcctgtccga
     2461 agcgctgatg gaggagcaga tcttccgccg cctgccgccg cagctctcga gcgcgctggt
     2521 gcacaagatt ccgtcgctgc tcgagtacaa gagcctcacc tcgctcgaca tgccggaagt
     2581 cgataccgtg ctgatcgagg atcccgatcc gaactgtccg ttcggcgcga aggaagccgg
     2641 gcaggggccg ctgcttccgg tgatgccggc ggtggcgaat gccatctacg acgccgtcgg
     2701 cgtgcgcatc gacgagctgc ccattacccc cgacaaggtt ctgagagccc tggaaatgaa
     2761 agcttcaggg aaggaagcgc gcgtgggccc atcccacttc cctgcagtgc cctatcccga
     2821 gccggtcatc gtgccaccgc cgtgggaagg cggcgatggc aacgcactaa aagattttga
     2881 aaaaaataaa atgaaaattg ctgcgccatg atgcggttgc cctggttcga gtaccgcgcg
     2941 ccgaagagcg tggccgaagc ggcgcgcatt ctcgccgccg aagggcccgc cgcggtgctg
     3001 atcgcgggag gcaccgacct gctgcccaac atgaagcggc gccagatggc gccgaaaatg
     3061 ctgatctctc ttaaaagaat aacccagcta agacaaaacg gcgcgatcct cggcgccggc
     3121 ctgactttgt ccgaggtggt gaagaagaaa gaactgccgg cggcgctgcg ccaggcggcg
     3181 gggcaggtgg cgaccgtgca cctgcgcaac atgggcacgc tcggcggcaa cctgtgcctc
     3241 gacacgcgct gcaactacta caaccagaac tacgagtggc gcaaggcgat cgacttctgc
     3301 ctgaagaagg acggcgacat ctgctgggtg gcgacggcga gcaagcgctg cgtggccatg
     3361 tcctccaccg attgcgctcc ggcgctgatg gcgctcggcg cgagagtcaa gctggtttct
     3421 tcgaaaaatg aaagagaact tgccctcgag gagctgtaca acaacgacgg catcgactat
     3481 ctcaggcgca ggccggacga gatcctgacc gagatcagaa ttcccgaaag cactgccagc
     3541 tgctactgga agctgcggcg ccgcggcgcc ttcgactttc cggtgctcgg cgttgccgct
     3601 gcactgaagg atggcaacgt caggattgcc ctgggtgcgg tggcatcgcg gccattcctg
     3661 gtcgacaagg cgagcgagtt tctcaaaggc aagcagctca ccgatgaagt gatcgaggaa
     3721 gccggccgca tcgtcgcctc gcgcgcgaag ccgatggaca acgccgacct cgacctttac
     3781 tggcgcaaag gcgtggccgc ggacttcgtc ggttacgcgc tgcgcgagct gcgcggcgac
     3841 gacatgcggg caacacgctt gcgcatagcg agatacgccg ttacctgaca tgcagaggaa
     3901 ttttgtttgt aagagcttga tgctataaaa cagggtcacg ccagggaggt gtgctccatg
     3961 aggaaatccg ctgcgccggc tagtgcgacg gttcgcgcgc tgctttgctg cctgttcacc
     4021 gtcgcgctcg atgcctcggg cgcgcagccg atgaagccgg aggaggttcc ggcgccgctc
     4081 aaaccgtgga ttccctgggc cctgcacggc acggcggaat ccccctgccc gctcatggcg
     4141 ggaaagccgg acgagcgcgc ctgcgcctgg cccgggctgc tcgagctcga catgcacgcg
     4201 accggcggca agttccgcca agcgatccgc gtctacgggc cgcctgccgc gccgctctgg
     4261 gtcgccgttc cagggggcgc cggccgctgg ccgcagaacg ttacaagcaa cggcaaagtc
     4321 gaggccgtga tcggcaggga cggcgttcct ggcgtctatc tcgcgcccgg cgagcatcgg
     4381 cttgccggcc agttcgcctg gagcgcgctg
//
LOCUS       DASPXW010000348         3704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1488298, whole genome shotgun sequence.
ACCESSION   DASPXW010000348 DASPXW010000000
VERSION     DASPXW010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1488298"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VEL04_07905"
     CDS             <1..240
                     /locus_tag="VEL04_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYT48329.1"
                     /translation="ADRIRVNCVNPVFSPETALSAEFAGGAVTDEAKQRFLATIPMGR
                     FSTPLDVANACLYLASDDAAFVSGVCIEVDGARCV"
     gene            complement(237..1529)
                     /locus_tag="VEL04_07910"
     CDS             complement(237..1529)
                     /locus_tag="VEL04_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HYT48330.1"
                     /translation="MQEQAIPLVLAGRDLMAGAQTGTGKTAAFALPILQRFAPQASAS
                     ASPARHPVRALVLTPTRELAVQVAASFRDYGKHVPLRSTVVYGGVDMNAQIEQLRKGV
                     EILVATPGRLLDHVQNKTVMFNQVSVLVLDEADRMLDMGFLPDIKRIIALLPRERQNL
                     LFSATFPEEIRTLAKTLLHDPAEVQVAARNAAAELVTHVLHPVAREKKRELLAYLIQA
                     RGLRQVLVFTGTRIGANRLAHQLRRDHIHADAIHGDKTQGEREAALQDFKAGKTAVLV
                     ATDVASRGLDIENLPQVINFDMPHSSEDYVHRIGRTGRAGLTGEAISLVAPEDMEAIG
                     AIERLIRKRIERVLVPGFQPGGTTVAALIGNEPKRRERRPAPVQTAKKNIDPIFSKPY
                     EPPAAPSAPAAAEKPEPQPSGKRRQPQVAALLGGLKRA"
     gene            complement(1630..2157)
                     /locus_tag="VEL04_07915"
     CDS             complement(1630..2157)
                     /locus_tag="VEL04_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48331.1"
                     /translation="MRLHQLKVEYDAEQDRLQMLVATSEGVELRLAMTRRFVKLLWPL
                     LVKLAEEASPRIRTQANPEARKALLGLEHEHAVSKADFSKPYDAGESERPLGEAPLLL
                     ARIQTGHDRNGQPVVALHPAQGQGVTLSFDSVLLHSVCRLLQAAVKKSDWDMELKLPG
                     TEPQETGERAARTLN"
     gene            complement(2154..2645)
                     /locus_tag="VEL04_07920"
     CDS             complement(2154..2645)
                     /locus_tag="VEL04_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HYT48332.1"
                     /translation="MKPPSFEAKIQPPGELERWLGALPRPLVFTNGVFDILHRGHVTC
                     LARARGLGAALLVALNGDASARRLGKEDGRPLNSLDDRLALVAALEPVDAVTWFDEDT
                     PEALIAACRPEVLAKGGDWPVERIVGARGVLARGGRVVSIPFEHERSTSGLLQRIRGN
                     IPS"
     gene            complement(2642..3277)
                     /locus_tag="VEL04_07925"
     CDS             complement(2642..3277)
                     /locus_tag="VEL04_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HYT48333.1"
                     /translation="MKRTLWLGAAAVAAGLLAGCGLVLIGAGGAAAYSSLEDRRSAGT
                     QIEDEGIELRSSNRIDQRYGFKVHVNVTSFNRSVLITGEVPDAGTREEVEKIVAAVPN
                     VRALANELQIGAATSLSARTGDSLITSNLKARFLGAKRFNPLHVKVVTEAGVVFLMGV
                     VTDAEANAAADIARTTDGVRKVVKVFEYCQSGEDLCRPRPAPPVDKSKPAA"
     gene            complement(3274..>3704)
                     /locus_tag="VEL04_07930"
     CDS             complement(3274..>3704)
                     /locus_tag="VEL04_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HYT48334.1"
                     /translation="ADAQHFAAELINRFEVERPPLAAVALTTDTSTLTSIANDYAYEQ
                     VFAKQLRAIGRRGDLLLAISTSGNSANVLEAVRAAHQLGVRVIALTGAGGGKLAAAMA
                     ADDVHLCVPHQSTARIQEVHLLVLHCLCDAIDFQLFGARG"
BASE COUNT          539 a         1261 c         1284 g          620 t
ORIGIN      
        1 gccgaccgca tccgcgtgaa ttgcgtcaac cccgtgttca gcccggagac ggcgttgtcg
       61 gcggagtttg ccggcggcgc ggtcaccgac gaggcgaagc agcgctttct cgccaccatc
      121 ccgatggggc gcttttcaac gccgctcgac gtcgcgaatg cctgccttta tctcgcctcc
      181 gacgacgcgg cgttcgtcag cggcgtctgc atcgaggtcg acggcgcgcg ctgcgtttag
      241 gcccgcttca ggccgcctaa gagcgcggcg acttgcggtt gcctgcgctt gccgctcggc
      301 tggggctcgg gcttctccgc cgcggcgggt gctgagggtg ccgccggcgg ctcgtacggc
      361 ttggagaaga tcgggtcgat gtttttcttt gctgtctgaa ccggagccgg gcggcgttcg
      421 cggcgcttcg gctcgttgcc gatcagcgcc gcgacggtgg tgccgccggg ttggaatccc
      481 ggcaccagca cgcgctcgat gcgcttcctg atcagccgct cgatcgcgcc gatcgcctcc
      541 atgtcctccg gagcgaccag cgagatcgcc tcgcccgtca agcctgcccg gccggtgcgg
      601 ccgatgcggt gcacgtagtc ctcggacgaa tgcggcatgt cgaagttgat cacctgcggc
      661 aggttttcga tgtcgagccc gcgcgaggcg acgtcggtcg cgaccagcac cgccgtcttg
      721 cccgccttga aatcctgcag cgccgcttcg cgttcgccct gggtcttgtc gccgtggatc
      781 gcgtcggcgt ggatatggtc gcgccgcagc tggtgggcga ggcggttggc gccgatgcgc
      841 gtgccggtga acaccagcac ctggcgcagg ccgcgggcct ggatcagata ggcgagcagc
      901 tcgcgctttt tctcgcgcgc cacggggtgc agcacatgcg tgaccagctc ggcggcggcg
      961 ttgcgcgcag cgacctgcac ctcggccgga tcgtgcagca gcgtcttggc gagggtgcgg
     1021 atctcttcgg gaaaggtggc ggagaacagc aggttctgcc gctccctggg cagcagcgcg
     1081 atgatgcgct tgatgtccgg caggaagccc atgtccagca tgcggtcggc ctcgtcgagc
     1141 accagcaccg agacctggtt gaacatcacc gtcttgttct gcacgtggtc gaggaggcgc
     1201 cccggcgtgg cgaccaggat ctccacgccc ttgcgcagct gctcgatctg cgcattcatg
     1261 tccaccccgc cgtacaccac ggtgctgcgc aaggggacgt gcttgccgta gtcgcggaag
     1321 ctcgcggcga cctgcactgc gagctcgcgc gtcggcgtga gcaccagcgc gcgcaccggg
     1381 tggcgcgcgg gcgaagccga agcgctcgcc tgcggcgcga agcgctgcag gatcggcaga
     1441 gcgaaggcgg cggtcttgcc agtgccggtc tgcgcaccgg ccatgaggtc gcgtccggcc
     1501 agcaccagcg ggatggcctg ctcctgcacc ggagtcggct tctcatagcc gagctcggca
     1561 accgccgcaa gcagcggcgg aatcagaccc aggttttcaa aagctgaagg gataaccttg
     1621 aattctaaat caattgagcg tgcgggccgc gcgctcgccg gtttcctgcg gctcggtgcc
     1681 cggaagcttc agttccatgt cccagtcgct tttcttcacc gccgcctgca gcaggcggca
     1741 gaccgaatgc agcagcaccg agtcgaacga cagcgtgacg ccctgaccct gcgccggatg
     1801 cagcgccact accggctggc cgttgcggtc gtggccggtc tggatgcgcg cgaggagcag
     1861 cggcgcctcg ccgagcggcc gctcgctctc gccggcatcg tagggcttgg agaaatccgc
     1921 cttggatacc gcatgctcgt gctcgagtcc gagcagcgcc ttgcgcgcct cgggattcgc
     1981 ctgggtgcgg atgcggggac ttgcttcctc ggcgagcttg accagcagcg gccagagcag
     2041 cttgacgaag cgccgcgtca tggcgagccg cagctcgacg ccttcgctcg tcgcgaccag
     2101 catctgcaag cggtcctgct cggcgtcata ttcgaccttc agctggtgca acctcatgag
     2161 ggaatattac ctctgatgcg ttgcaagagc ccgctcgtcg aacgctcgtg ctcgaagggg
     2221 atcgacacca cgcggccgcc gcgcgccagc acgccgcgcg cgccgacgat gcgctccacc
     2281 ggccagtcgc ctcccttggc gagaacctcg gggcggcagg ccgcgatcag cgcctcgggc
     2341 gtgtcctcgt cgaaccatgt gaccgcgtcg accggctcga gcgccgcgac cagcgcgagc
     2401 cggtcgtcga gcgagttgag cgggcggccg tcttccttgc ccagacgccg ggccgaggcg
     2461 tcgccgttca gcgccacgag aagcgcggcg ccgaggccgc gcgcgcgggc gagacaggtg
     2521 acatggccgc gatgcaggat gtcaaacacg ccgttggtga agaccagcgg gcgcggcagg
     2581 gcgccaagcc agcgctcgag ctcccctgga ggttggatct tggcttcgaa cgacggcggc
     2641 ttcacgccgc gggcttggat ttgtcgaccg gcggcgccgg gcgcggccgg cacagatctt
     2701 cgcccgattg gcagtattcg aacactttca ccaccttgcg cacgccgtcg gtggtgcgcg
     2761 cgatgtcggc cgcagcgttc gcttcggcgt cggtgacgac gcccatcagg aacaccacgc
     2821 ccgcctcggt gaccaccttg acgtgcagcg gattgaagcg cttggcgccg agaaatctcg
     2881 ctttgaggtt ggaagtgatc aatgagtcgc cggtgcgcgc gctgagcgag gtcgcggcgc
     2941 cgatctgcag ctcgttcgcc agcgcgcgga cgttcggcac cgcggccacg atcttctcga
     3001 cctcctcgcg cgtgcccgcg tcgggcactt ccccggtgat cagcaccgag cggttgaagg
     3061 aagtcacgtt gacatggact ttgaagccgt agcgctggtc gatgcggttc gaagagcgca
     3121 actcgatccc ctcgtcctcg atctgcgtcc cggcgctccg gcgatcctcg agcgagctgt
     3181 aggccgccgc gccgcccgcg ccgatcaaca ccaacccgca gccggcgagc agtcccgccg
     3241 cgacggcagc cgcgcccagc cagagcgtgc gtttcatccc cttgcgccga acagctggaa
     3301 atcgatcgcg tcgcacaggc aatgcagcac cagcagatga acttcctgga tgcgcgcggt
     3361 gctctggtgc gggacgcaga ggtgcacgtc gtcggctgcc atcgccgccg ccagcttgcc
     3421 tccgcccgcg ccggtcagcg cgatgacgcg cacgccgagc tggtgcgccg cccgtaccgc
     3481 ttcgagcacg ttggccgaat tgccgctggt ggagatcgcg agcagcaggt cgccgcgccg
     3541 gccgatcgcg cgcagctgct tcgcgaacac ctgctcgtag gcgtaatcgt tggcgatcga
     3601 ggtcagcgtc gaggtgtcgg tggtgagcgc gaccgcggcg agcggcggcc gttccacctc
     3661 gaagcgattg atgagctcgg cggcgaagtg ctgcgcgtcg gcgg
//
LOCUS       DASPXW010000349         3796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_855100, whole genome shotgun sequence.
ACCESSION   DASPXW010000349 DASPXW010000000
VERSION     DASPXW010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855100"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..171
                     /locus_tag="VEL04_07935"
     CDS             4..171
                     /locus_tag="VEL04_07935"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HYT48335.1"
                     /translation="MDERGAIINIADMEPHSERTHCAARPASYALEMNRGWFAARGIK
                     PGMRLGGIPRK"
     gene            complement(175..750)
                     /locus_tag="VEL04_07940"
     CDS             complement(175..750)
                     /locus_tag="VEL04_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HYT48336.1"
                     /translation="MNRTVAAGVVVLIVLLAAAGAFTGHWIQVGQHGWWQFAMRWLHV
                     TAGVMWIGLLWYFNFVQTPSMPKIPDEQKPAISKVIAPAALFWFRWAAMATIILGLIL
                     AWMNGYLSKALGLQKDYFGIGLGMWLGVIMWLNVWLIIWPNQKKALGIVQVSPEEKAA
                     AAKMAGLTSRINTMLSIPMLYFMVAQSHGGL"
     gene            838..1614
                     /gene="otnI"
                     /locus_tag="VEL04_07945"
     CDS             838..1614
                     /gene="otnI"
                     /locus_tag="VEL04_07945"
                     /EC_number="5.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HYT48337.1"
                     /translation="MPKLAANLSMLFKEVPFLDRFAAAAGAGFRAVEYQYPYEWRPED
                     VAARARDAGVQILLHNMPRGEAERGEHGTACLPGREARFREDLERAVEYARAVGCTRL
                     HCMAGVAPAGAERAALQAAYVANLKYAAGRLQAEGMQVLIEPLSARTVAGYFLTGSAQ
                     AARALDEVGAANVLIQYDVFHMQIMEGNLAASIERLLPRIGHLQIADVPGRHEPGSGE
                     INFDFLLRHVDAIGYSGWVGCEYNPQGDTAEGLKWAKPYL"
     gene            1593..3077
                     /locus_tag="VEL04_07950"
     CDS             1593..3077
                     /locus_tag="VEL04_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HYT48338.1"
                     /translation="MGEALPVGNPARQREVAHALRAFLPARAVLYEEEDTRPYECDGL
                     TAYRQLPMIVALPESEAQVQRVLKTCAAMRVPVVPRGAGTGLSGGALPPGDGVLLSMA
                     KFMRVIRLDARSRIAVVQPGVRNAAISEAAAPYGLYYAPDPSSQIACTIGGNVAENSG
                     GVHCLKYGLTVHNVVALRGFTIDGDAVAFGSEAPDAAGYDLLALAVGSEGMLLVVTEV
                     SVKLLPKPQCARVIMASFDDVEKAGSAVAEVIAAGIIPAGLEMMDRPATHAVEQFVGA
                     GYDLAAAAILLCESDGTPEEVDDEISRMKAVLERAGATRMTVSSDEAERLRFWSGRKA
                     AFPAVGRISPDYYCMDGTIPRKRLGEVLNFIAAMEKKYGLRCPNVFHAGDGNLHPLIL
                     FDANDAGELARTEQFAAEVLEKCVAVGGTITGEHGVGVEKINQMCVQFRPDELELFHA
                     VKRAFDPHGLLNPGKAVPTLARCAEYGRLRVHGGQLPHPDLPRF"
     gene            3074..>3796
                     /gene="glcE"
                     /locus_tag="VEL04_07955"
     CDS             3074..>3796
                     /gene="glcE"
                     /locus_tag="VEL04_07955"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HYT48339.1"
                     /translation="MTLEQLRQRIGEAWARATPLRLRGGGSKDFYGNAPRGELLDTRG
                     YAGIVNYEPTELVVTARCGTPLAELEKILHDHGQCLPFEPPHFGPAATLGGCVAAGLS
                     GPRRAAAGAVRDFMLGAKIVDGRGRLLGFGGEVMKNVAGYDVSRLLAGSLGTLGLIAE
                     VSLKLLPRPPCELTLRLEMPQARALEALSRWAGQPLPISASAWRDSELSVRLSGAAPA
                     VRAAAQKLGGEALAEADFFWRGV"
BASE COUNT          631 a         1234 c         1325 g          606 t
ORIGIN      
        1 ttcatcgacg agcgcggcgc gatcatcaac atcgccgaca tggagccgca cagcgagcgc
       61 acgcactgcg ccgcgcgccc ggcgagctac gcgctggaga tgaaccgggg ctggtttgcg
      121 gcgcggggta tcaaaccggg aatgcgcctc ggcggtatcc cacgcaaata gcggtcagag
      181 gccgccgtgc gactgggcca ccatgaagta cagcatcggg atcgacagca tggtgttgat
      241 acgcgacgta aggcccgcca ttttcgcagc ggccgccttt tcctcggggc tgacctgcac
      301 gatgccgagc gccttcttct ggtttggcca gatgatcaac cagacgttga gccacatgat
      361 cacaccgagc cacatgccga gcccgatgcc gaaatagtcc ttttgcaggc caagcgcctt
      421 gctgaggtag ccgttcatcc aggcgaggat caggcccaaa atgatggttg ccatcgccgc
      481 ccaacggaac cagaaaagcg ccgcgggcgc gatcaccttg ctgatcgccg gcttctgctc
      541 gtcggggatc ttgggcatcg agggcgtctg gacgaaattg aaataccaca ggagaccgat
      601 ccacatcaca cctgctgtga catgcaacca gcgcatggcg aattgccacc agccgtgctg
      661 accaacttga atccaatgac cggtgaaggc gccggcggcg gcaagcaaga cgatgaggac
      721 gaccacgcca gcggcgactg tacggttcaa tgattggaag aacgcaccca tggtttcgcc
      781 ttttcgtatg atgggttaga ggagaaaagt ctagcacgcg tgagttatgc tcgcacaatg
      841 ccgaagctcg cagcgaatct ttccatgctg ttcaaggaag tgccattcct ggatcgcttc
      901 gcagccgcag cgggcgccgg gtttcgcgcg gtcgaatacc agtatccgta cgagtggcgg
      961 ccggaggacg ttgcggcgcg cgcgcgcgac gccggggtgc agattctgct gcacaacatg
     1021 ccgcgcggcg aggcggagcg gggcgagcac ggcacggcgt gcctgccggg ccgggaagcg
     1081 cgctttcgcg aggacctcga gcgcgccgtg gaatacgcac gcgccgtggg ttgcacgcgc
     1141 ctgcattgca tggcgggtgt ggcgcctgcg ggtgcggagc gtgccgcgct gcaggccgcc
     1201 tatgtcgcca acctgaaata cgctgccgga cggttgcagg ccgaaggcat gcaggttctg
     1261 atcgagccgc tcagcgcgcg cacggtggcc ggctatttcc tcacgggaag cgcgcaggcc
     1321 gcgcgcgcgc tcgacgaggt cggggccgcg aacgttctca tccagtacga cgttttccac
     1381 atgcagatca tggaaggcaa tctggcggcg agcatcgagc ggctgctgcc gaggatcggc
     1441 cacctgcaga tcgccgatgt tcccgggcgc cacgagccgg gcagcggaga gatcaatttc
     1501 gatttcctgc tgcgccacgt cgatgccatc ggctactcgg gctgggtggg ttgcgaatac
     1561 aatccgcagg gtgacaccgc ggaaggcctg aagtgggcga agccctacct gtaggcaatc
     1621 ctgcaaggca acgcgaagtg gcgcatgcgc tgcgcgcctt cctgccggcg cgggcggtgc
     1681 tgtacgagga agaggacacg cgaccctatg aatgcgatgg cctcaccgcg taccgccagt
     1741 tgccgatgat cgtggcgctg ccggagagcg aggcgcaggt gcagcgggtg ctgaaaacct
     1801 gcgccgcgat gcgcgtgccg gtggtgccgc ggggcgcggg cacggggctc tccggcggtg
     1861 cgctgccccc gggcgacggc gtgctgctct cgatggcgaa attcatgcgc gtcatccggc
     1921 tcgatgcgcg ctcgcgcatc gcggtggtgc agccgggcgt gcgcaatgcc gcgatctcgg
     1981 aggcggctgc gccctacggg ctttactatg cgcccgatcc gtcgagccag atcgcctgca
     2041 ccatcggcgg caacgttgcg gaaaattcgg gcggcgtgca ttgcctgaag tacggcctca
     2101 cggtgcacaa cgtggtcgca ttgcgcggct tcacgatcga tggcgatgcg gtcgctttcg
     2161 gtagcgaggc gccggacgcc gccggctacg atctgctcgc gctcgccgtc ggctcggagg
     2221 gcatgctgct cgtcgtgacc gaggtgagcg tcaagttgct gccgaagccg cagtgcgccc
     2281 gcgtcatcat ggcctcgttc gacgacgtcg agaaggcggg cagcgcggtc gccgaggtca
     2341 tcgccgccgg catcattccc gccggcctcg agatgatgga ccggcccgcg acgcacgccg
     2401 tggagcagtt cgtcggcgcc ggctacgacc tggcggcggc ggcgatcctg ctgtgcgaat
     2461 ccgacggcac gccggaagag gtcgacgacg aaattagtcg catgaaggca gtgctcgagc
     2521 gcgcgggcgc gacacgcatg accgtatcga gcgatgaggc cgagcggctg cggttctggt
     2581 ccggccgcaa ggccgcgttc cccgcggtcg gccgcatctc gcccgattac tactgcatgg
     2641 acggcaccat tccgcgcaag cgcctcggcg aagtgctcaa cttcatcgcc gccatggaaa
     2701 agaagtacgg cctgcgctgc ccgaacgttt tccatgccgg cgacggcaac ctgcatccgc
     2761 tgatcctgtt cgacgccaac gacgccggcg agctcgcgcg caccgagcag ttcgccgccg
     2821 aggtgctcga gaagtgcgtt gcggtgggcg gcacgatcac cggcgagcac ggcgtcggcg
     2881 tcgagaagat caaccagatg tgcgtgcagt tccggcccga cgagctggag ctgttccacg
     2941 cggtgaagcg cgccttcgac ccgcacggtc tgctcaaccc cggcaaggcg gtgccgacgc
     3001 tcgcgcgctg cgccgaatac ggccggctgc gcgtgcacgg cggacagttg ccgcatcccg
     3061 atctgccccg gttttgactc tggaacaatt acgccagcgc atcggtgaag cttgggctcg
     3121 cgcgacgccg ctgcggctgc gcggcggcgg cagcaaggac ttctacggca atgcgccgcg
     3181 cggcgagctg ctcgacacgc gcggctatgc gggcatcgtg aactacgagc cgaccgagct
     3241 cgtggtcacc gcgcgctgcg gcaccccgct tgcggagctt gaaaaaatcc tgcatgacca
     3301 cgggcagtgc ctgccgttcg agccgccgca tttcggaccc gcggcgacgc tcggcggctg
     3361 cgtcgccgcg ggactctcgg ggccgcgccg ggcggccgca ggcgcggtgc gcgatttcat
     3421 gctcggggcg aagatcgtcg acgggcgcgg gcgcctgctc ggcttcggcg gcgaggtgat
     3481 gaaaaacgtc gccggctacg acgtgtcgcg cctgctcgcc ggctcgctcg gcacgcttgg
     3541 cctcatcgcc gaagtgtcgc tcaagctcct gcctcggccg ccatgcgagc tcaccctgcg
     3601 cctcgagatg ccgcaggcgc gcgcgctcga ggcgctcagc cgctgggccg gccagccgct
     3661 gccgatctcg gcgagcgcct ggcgagacag cgagctctcg gtgcggctgt cgggcgccgc
     3721 gcccgcggtg cgcgccgcgg cgcagaagct cggcggcgaa gcgctcgcgg aagcggattt
     3781 tttctggcga ggcgta
//
LOCUS       DASPXW010000350         3293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_104451, whole genome shotgun sequence.
ACCESSION   DASPXW010000350 DASPXW010000000
VERSION     DASPXW010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104451"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1053)
                     /locus_tag="VEL04_07960"
     CDS             complement(<1..1053)
                     /locus_tag="VEL04_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HYT48340.1"
                     /translation="MDAQVLIVGAGPTGLTLAIDLGRRGVRALVIEQKDAPQFLPKME
                     RCNARTMEIYRRMGIVEEVRAAGLPAHCPMDIFVVLSLVEPALVHHVHPSVAEAKAQI
                     AKSEDGSQPLEPYQLISQYTLEPLLKSIAETLPSVTVRYGCELVSFEQDGSSVTAQVK
                     SSDGALSTLRAAYMVGCDGGGSLVRRQLGINLSGEANIQQLRQALYYCKDLYERIPIG
                     KGRHYHVADAEGSFLIVQDSTRHFTLHSVVETDAEMAGMFERVVAMPVKYEMLYVGQW
                     KMNLLLADRYGEDRVFLAGDAVHLVIPTGGLGMNTGVGDAIDLSWKLAATLEGWGGPN
                     LLSSYEIERRQVGERNV"
     gene            complement(1221..3230)
                     /locus_tag="VEL04_07965"
     CDS             complement(1221..3230)
                     /locus_tag="VEL04_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014618140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HYT48341.1"
                     /translation="MVSLARRFHKLLIANRGEIACRVMRTAKRMGLRTVAVYSDADRN
                     AMHVALADEAVLIGPAPAKDSYLHIDAIIDAAQATGAEAVHPGYGFLSENADFAQACA
                     DAGLVFVGPSAATIRLMGSKSAAKALMESSGVPVVPGYHGEDQSMATLQAAADRIGYP
                     VLVKASAGGGGRGMRLVGNADGLAEAIASAKREAASSFGNDQILIEKYLDRPRHIEVQ
                     VFGDGDGNVVPLFERECTLQRRHQKVVEEALAAAITPERRAAMSAAARAAAQAAGYLG
                     AGTVEFIADAAGFHFIEMNTRLQVEHPVTEMVTGIDLVEWQLRVAMGETLPLRQDEIT
                     ANGHAIEARIYAEDADKGFLPAAGTIRAWREPAGDGIRIDTGFRAGDTITPYYDALLA
                     KLIAWGADRPQALGRLVEALDAFEIAGVTTNLAFLKALLRHPQVVRDEIDAGFIEREI
                     STLTRSAPSLGALDLAAACAAVLLREQREQVSEESPWDRTDGWTLAGRRSRRLSFRGG
                     GERYDAVLWYGRDGLSMEFGGTNDRLQFVPREGAVFDMCLGNALERVSVAWSGRDLDL
                     STLRGRCKLHWIDPFVADISEAAATARIVAPMPGTVTRILAEPGVGLQRGAPLIVLEA
                     MKMEHTLRAPADGRLKALKCAVGDFVQEGTELADFEAAGEMETAK"
BASE COUNT          562 a         1138 c         1044 g          549 t
ORIGIN      
        1 cacgttgcgc tcgcccactt gccggcgctc gatctcgtag gaggagagca gattgggccc
       61 gccccatccc tcgagcgtcg ccgcaagctt ccacgacaga tcgatggcgt cgccgacgcc
      121 ggtattcatc ccgagcccgc cggtcggaat gacgaggtgc acggcgtccc cggccagaaa
      181 aacccggtcc tcgccgtagc ggtcggcgag cagcagattc atcttccact gcccgacata
      241 gagcatctcg tatttcaccg gcatggcaac gacgcgctcg aacatgccgg ccatctccgc
      301 atccgtctcg acgacggagt gcagggtgaa atgccgggtc gaatcctgga cgatcaggaa
      361 actcccttcg gcgtcggcga cgtgatagtg ccgccccttg ccgatcggga tgcgctcgta
      421 gagatccttg caataataga gcgcctgccg cagctgctgg atgttggcct caccgctgag
      481 gttgatgccg agctgccggc gcaccaggct gccgccgccg tcgcagccga ccatataggc
      541 ggcgcgcagc gtggacaaag cgccgtcgct gctcttgacc tgcgcagtga ccgaactgcc
      601 atcctgctcg aatgacacga gctcgcatcc gtagcgcacg gtgacgctcg gcaacgtctc
      661 cgcgatcgat ttcagcagcg gctcgagcgt atattgcgag atgagctgat agggctcgag
      721 cggctggctg ccgtcctcgg atttggcgat ctgcgccttt gcctcggcca cggacggatg
      781 cacgtggtgc accagcgccg gctcgaccag cgacaacacg acgaagatgt ccatggggca
      841 atgcgcgggg agcccagcgg cgcgaacctc ctccacgatg cccatgcgcc ggtagatctc
      901 catggtgcgg gcgttgcagc gctccatctt cgggaggaat tgcggagcat ccttctgctc
      961 gatcaccagc gcgcgcacgc cgcgccgccc gaggtcgatg gcgagcgtca agccggtggg
     1021 gcccgctcca acgatgagaa cctgtgcgtc catagatttt gactgacctc accgaggcga
     1081 acgaatccga ctcatcagct agtatcctgt cgtccccgcg aaagcgggga cccataacca
     1141 ccgcctctgg aatatgggtc cccgctttcg cggggacgac agcggacagt gtgtcgccga
     1201 accaatcggc cgggttgcgg tcatttcgct gtttccattt ctcccgccgc ttcgaaatcg
     1261 gcgagctccg ttccttcctg cacgaagtcg ccgacggcgc atttgagtgc cttcaagcgg
     1321 ccgtcggctg gcgcgcgcag cgtgtgctcc atcttcatcg cctccagcac gatgaggggc
     1381 gcgccgcgct gcaaaccgac gcccggctcg gcgaggatgc gcgtcaccgt accgggcatc
     1441 ggcgcgacga tgcgcgcggt tgccgcagct tcggaaatat ccgccacgaa cgggtcgatc
     1501 caatgcagct tgcaacggcc gcgcagcgtg ctcagatcga gatcgcggcc ggaccatgcc
     1561 acggacaccc gctcgagcgc gttgccgaga cacatgtcga acacggcgcc ctcgcgcggg
     1621 acaaattgca agcgatcgtt cgtgccgccg aattccatgc tcaagccatc gcggccgtac
     1681 cacagcaccg cgtcgtatcg ctcgccgccg cctcgaaagc tcaagcggcg actgcggcgc
     1741 ccggcgagcg tccagccgtc ggtccgatcc cagggcgact cctccgaaac ctgctcgcgc
     1801 tgttcgcgca gcagcaccgc cgcgcaagcc gcggcaaggt cgagcgcccc gagcgagggc
     1861 gcgctcctcg tcagcgtgga gatttcccgc tcgatgaagc cggcgtcgat ctcgtcgcgg
     1921 acgacttgcg ggtggcgcag cagcgccttg aggaaagcaa gattggtggt gacgcctgcg
     1981 atctcgaacg catcgagcgc ctcgaccaga cggccgagcg cctgcggccg atcggctccc
     2041 caggcgatca gcttcgccag caacgcatcg tagtacggcg tgatcgtatc gcccgcgcga
     2101 aagccggtat cgattctgat tccgtcccca gccggctcgc gccatgcacg gatggttccg
     2161 gccgcgggca gaaatccctt gtcggcgtcc tcggcataaa tgcgcgcctc gatcgcatgg
     2221 ccgttggccg tgatctcgtc ctgccgcagc ggtagcgttt ctcccatggc gacgcgcaat
     2281 tgccactcca cgaggtcgat gcccgtgacc atctccgtca cgggatgctc gacctgcagc
     2341 cgcgtgttca tctcgatgaa atgaaaaccg gccgcatccg cgatgaactc gacggtgccg
     2401 gcgccgagat agcccgcagc ctgggcggcg gcgcgcgccg ccgccgacat cgccgcgcgc
     2461 cgttcggggg tgatggcggc cgcaagcgcc tcctccacca ccttctgatg acggcgctgg
     2521 agcgtgcatt cgcgctcgaa caacggcacc acgttgccgt cgccgtcgcc gaacacctgc
     2581 acttcgatat gccgcggcct gtccaggtat ttctcgatca ggatttggtc attcccaaac
     2641 gacgacgccg cttcgcgctt ggcgctcgcg atcgcttcgg cgagcccatc ggcgttgccg
     2701 accaggcgca ttcctcgacc gccgccgccc gccgatgcct tgaccagcac cggatagccg
     2761 atgcgatcgg cggcggcttg cagcgtcgcc atgctctgat cctcgccgtg atagccgggc
     2821 acgaccggca cacccgacga ctccatgagc gccttggccg ccgatttcga gcccatcagg
     2881 cggatggtgg cggcagaagg gccgacgaaa accaggccgg cgtccgcgca ggcttgcgcg
     2941 aagtcggcgt tctcggagag aaagccgtag ccgggatgga ccgcttcggc gcccgtcgcc
     3001 tgcgccgcgt cgatgatggc gtcgatgtga aggtagctgt ctttcgccgg cgccgggccg
     3061 atgagcaccg cctcgtcggc gagcgcaacg tgcatggcgt tgcgatcggc gtcggaatag
     3121 accgccacgg tgcgcaatcc catgcgtttg gccgtacgca tgacccgaca ggcgatctcg
     3181 ccgcgattgg cgatcaacaa cttgtggaag cgccgagcga gcgacaccat cacatcctga
     3241 agatgccgaa ccgcgtgggc tcgggcggcg gcgcattgag cccggcagcg agc
//
LOCUS       DASPXW010000351         4076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_982453, whole genome shotgun sequence.
ACCESSION   DASPXW010000351 DASPXW010000000
VERSION     DASPXW010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982453"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VEL04_07970"
     CDS             <1..424
                     /locus_tag="VEL04_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48342.1"
                     /translation="FVYLCGPVRRASAQLRTYREGIPPLDALSALYEFADGTSGMLAT
                     VRATPFYWRVHVFGSEGSAEALGETELVLRMSGKQTERIQLDNTNSLRAELEAFADAV
                     ENRAPYPISEEQMLATVSAFEATVRSVESGEPVIIPGS"
     gene            complement(408..1256)
                     /locus_tag="VEL04_07975"
     CDS             complement(408..1256)
                     /locus_tag="VEL04_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HYT48343.1"
                     /translation="MRRCLTPGPGLRLGIDLGGTKIEAIALDPGGNEVFRKRVPAPRG
                     DYAATLAAVVSLVGEIGKGSVGVGIPGALSRVTGLVKNANSTWLIGKPLKQDLERALQ
                     REIRIENDANCFALSEAVDGAGRDAHVVFGVILGTGVGGGISISKKIVTGANAIAGEW
                     GHNPLPLPTGSDLPLPECYCGRKGCIETYLSGPAFARDHENLDRYHERLARSLAGVIN
                     ILDPEVIVLGGGMSNIPSLYTEVPRLWQRYVFSDHVATRLARPVHGDSSGVRGAAWLW
                     DVNSRE"
     gene            complement(1253..2005)
                     /locus_tag="VEL04_07980"
     CDS             complement(1253..2005)
                     /locus_tag="VEL04_07980"
                     /inference="COORDINATES: protein motif:HMM:NF016658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HYT48344.1"
                     /translation="MAVEQQGGEQQLAEALEAVKAAHFDAATGACDFAALVISREHGR
                     LAACLASFAEFDPHRLRIAAQTAFWLNLYNACVLRDALELEVEGFFTRQRARVAGLGW
                     SLDDIEHGLLRGNAPKSGSFSAPMKRSDPRLAYVPLAFDERIHFALYSARRSSPPLRI
                     FHSGALEDELEDATRDYLLARVRVKDEDYRAKLRLPMIFKWYADDFGDERGLLEFVLA
                     RLDEEVAELVDRCEGRVKFKYLEFDATLNAKL"
     gene            2046..3644
                     /locus_tag="VEL04_07985"
     CDS             2046..3644
                     /locus_tag="VEL04_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HYT48345.1"
                     /translation="MRHFGSFDFVIAGAGSAGCLLANRLSRDPDTRVLLLEAGGRDDW
                     FWIDIPVGYLYTIANPRTDWCYKTEPDQHLAGRSIHYARGRVLGGCSSINAMIYMRGQ
                     KEDWDHWASLGNRGWSWDEVLPIFKSLEDYERGATDGYGAGGEVRIEDPRVRWEIVDA
                     WREAAAECGIPRVKVFNNGDNFGCAYFQMNQRRGRRWSATNAFLRPVLHRKNLTVATG
                     ALVSKIRLTGKQASGVEFTKDRDSFYAEANSETILAAGAIGSPQLLQLSGIGPGDLLQ
                     KLQVPVAHSLPGVGENLQDHLQIRMQYKVRNVRTLNGMANSLLGKAAMALEYLMFRTG
                     PLTMPPSQAGAFAKSDPSEPTPNIEWHVQPLSLDKFGEPLHGFPAITPSVCNLRPASR
                     GRVRIKSPDPSAYPEIRLNYLGEDIDRRVAVDAMRFTRRIMCAKALAKYDPEEYRPGL
                     AFQDNLSLEKAAGELGTTIFHPVGTCKMGRDPMAVVDDELRVHGVARLRVIDASIFPR
                     ITSGNTNAPTYVIAEKGARSILKT"
     gene            3687..>4076
                     /locus_tag="VEL04_07990"
     CDS             3687..>4076
                     /locus_tag="VEL04_07990"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HYT48346.1"
                     /translation="MKRIVLLLATNIAVLLVLSVIVSVLGLDRWLMAEGIDYGSLLAF
                     SAVFGFGGAFISLLMSKTIARWSVGAQVIDGSEGTTQYWLVQTVKKLAEHAGIGMPEV
                     AVYEGPANAFATGAFRDAALVAVSSGLL"
BASE COUNT          716 a         1380 c         1305 g          675 t
ORIGIN      
        1 cttcgtctac ctgtgcgggc cggtgcgccg cgcctccgcg cagcttcgca cctacaggga
       61 aggcatcccg ccgctcgacg cgctgagcgc gctgtacgaa ttcgccgacg gcacgagcgg
      121 catgctcgcc accgtgcgcg cgacgccttt ctactggcgc gtgcatgtgt tcggcagcga
      181 aggctcggcc gaagcgctgg gcgaaaccga gcttgtcctg aggatgagcg gcaaacaaac
      241 cgaaagaatt caactggaca acacaaattc attacgtgct gagctcgaag ccttcgccga
      301 cgcggtcgag aaccgcgcgc cctacccgat ttccgaagag cagatgctcg cgaccgtgtc
      361 ggccttcgaa gcaaccgtcc gttcggtgga aagcggcgag ccggtgatca ttcccgggag
      421 ttgacgtccc acagccaggc cgcgccgcgc acgccgctcg agtcgccgtg gacgggtcgc
      481 gcgaggcgcg tcgcgacgtg gtcggaaaac acgtaccgct gccacaggcg cggcacctcg
      541 gtgtacaggc tcggaatatt ggacatgccg ccgcccagca cgatgacctc cgggtcgagg
      601 atattgatca cgccggcaag cgagcgcgcc agccgctcgt gataccgatc gagattctcg
      661 tggtcgcgcg cgaaagccgg gcccgagagg taggtctcga tgcagccctt gcggccgcag
      721 tagcactccg gcagcggcag atcggacccg gtcggcagcg gcagcggatt gtgtccccat
      781 tcgccggcga tcgcgttcgc cccggtgacg attttcttgc ttattgaaat cccgccgcca
      841 acacccgtac ccaggatgac gccgaacacg acgtgcgcat cgcggcccgc gccgtccacg
      901 gcctcggaga gcgcgaagca gttcgcgtcg ttttcgatcc tgatttccct ttgcagcgcg
      961 cgctccagat cctgcttcag cggcttgccg atgagccagg tggagttcgc gttcttcacc
     1021 agcccggtta cgcgggaaag cgccccggga atgcccacgc cgacgctgcc tttcccgatc
     1081 tcgccgacca gcgacacgac cgcggcaagc gtcgccgcgt aatctcccct cggggcggga
     1141 actcttttcc ggaagacctc gttcccgccg gggtcgagcg ctatagcctc gatcttggtg
     1201 ccgccgaggt cgatgccgag ccgcaggcca gggccgggcg tcaggcagcg cctcagagtt
     1261 tggcgttgag agtcgcgtca aactccaggt acttgaactt gacccgtccc tcgcaccggt
     1321 cgaccagctc ggccacctcc tcgtccagcc gcgccagcac gaactcgagc aggccgcgct
     1381 cgtcgccgaa atcgtccgcg taccacttga agatcatcgg caggcgcagc ttggcgcggt
     1441 agtcctcgtc cttcacgcgc accctcgcca gcaggtagtc gcgggtcgcg tcctcgagct
     1501 cgtcctccag ggccccactg tgaaagatgc gcagcggagg cgatgagcgc cgcgcgctgt
     1561 acagggcgaa atggatgcgc tcgtcgaagg cgagcggcac gtaggcgagc cgcgggtcgg
     1621 acctcttcat cggcgcgctg aagctgcctg acttgggcgc gttgccgcgc agcaggccgt
     1681 gctcgatgtc gtcgagcgac cagcccaggc ccgcgacccg cgcgcgctgg cgcgtgaaaa
     1741 agccctcgac ctcgagctcg agcgcatcgc gcagcacgca ggcgttgtag agattgagcc
     1801 agaaagcggt ctgcgcagcg atgcgcagcc ggtgcggatc gaactcggcg aacgaggcaa
     1861 ggcaggcggc gagccggccg tgctcgcgcg aaatcaccag cgcggcaaaa tcgcaggcgc
     1921 cggtcgcggc gtcgaaatgc gcggccttca ccgcctcgag cgcctccgcg agctgctgct
     1981 ccccgccctg ttgttccacg gccattcccg cttcctccgc tcgttacaat accccagggg
     2041 gaaacttgcg gcacttcgga agcttcgact tcgtgatcgc cggcgccgga agcgcgggct
     2101 gcctgctggc gaaccgcctg tcgcgagatc ccgacacacg ggtgctgctc ctcgaagccg
     2161 gcgggcgcga cgactggttc tggatcgaca tcccggtcgg ctacctctac accatcgcca
     2221 acccgcgcac cgactggtgc tacaaaaccg agcccgacca gcacctcgcg ggccgctcga
     2281 tccactacgc gcgcggccgc gtgctgggcg gctgctcgtc gatcaacgcc atgatctaca
     2341 tgcgcggcca gaaggaggac tgggaccact gggcgtctct cggcaaccgc ggctggtcgt
     2401 gggacgaggt gttgccgatt ttcaaatccc tcgaagatta tgagcgcggc gcgactgacg
     2461 gctatggcgc cggcggcgag gtgcgcatcg aggacccgcg cgtgcgctgg gagatcgtcg
     2521 acgcctggcg cgaagccgcc gccgagtgcg gcatccccag ggtcaaggta ttcaacaacg
     2581 gcgacaactt cggctgcgcc tatttccaga tgaaccagcg ccgcggccgg cgctggagcg
     2641 cgaccaacgc gttcctgcgc cctgtgctgc atcgcaagaa cctgacggta gcgacaggcg
     2701 ctttggtttc aaaaataaga ctcacgggga aacaggcgag tggcgtcgag ttcacgaaag
     2761 acagggattc cttctatgca gaagcaaaca gcgagaccat tctcgcggcc ggcgcgatcg
     2821 gctcgccgca gcttttgcag ctgtccggaa ttggtccagg agacttgctg cagaagctgc
     2881 aagttcctgt cgcccattcc ctccccgggg tcggcgagaa cctgcaggac cacctgcaga
     2941 tccgcatgca atacaaggtc aggaacgtcc ggacgctgaa tgggatggcg aacagcctct
     3001 taggcaaggc agcgatggcg ctcgagtacc tcatgttccg caccggtccg ctcaccatgc
     3061 cgccctcgca ggcgggcgcg ttcgcgaaga gcgacccttc cgagccgacg ccgaacatcg
     3121 agtggcacgt gcagccgctg tcgctcgaca agttcggcga gccgctgcac ggcttcccgg
     3181 cgatcacgcc gagcgtctgc aacctgcgcc cagcctcgcg cggccgggtg cgcatcaagt
     3241 cgcccgaccc gtcggcgtat ccggaaataa gattgaatta cctcggtgag gacatagacc
     3301 ggcgggttgc ggtagacgcg atgcggttca cgcgtcgcat catgtgcgca aaggcgctgg
     3361 cgaaatacga tccggaggaa tacaggcccg gtttggcttt tcaggacaat ctttctcttg
     3421 agaaggcggc aggcgagctc ggcaccacca tcttccaccc ggtaggcacc tgcaagatgg
     3481 ggcgcgaccc gatggcggtg gtcgacgacg agctgcgcgt gcacggcgtg gcgcggctgc
     3541 gggtgatcga cgcctcgatt ttcccgcgca tcacctcggg caacaccaat gcgcccacct
     3601 acgtgatcgc ggaaaagggt gcgcgctcga tcttgaaaac gtagaatccc gcccctatct
     3661 acgtgccgtc ccaggagagc aactccatga aacgcatcgt ccttctcctc gccaccaata
     3721 tcgcggtcct gctggtgctt tcggtgatcg tcagcgtgct cggcctcgac cgctggctga
     3781 tggccgaggg catcgactac ggctcgctgc tcgcgttctc cgccgtcttc ggcttcggcg
     3841 gcgcgttcat ttcgctgctg atgtccaaga ccatcgccag gtggtcggtc ggcgcgcaag
     3901 tcatcgacgg ctcggaaggc accacgcaat actggctggt gcagacagtc aagaagcttg
     3961 ccgagcacgc cggcatcggc atgcccgaag tcgcggtcta cgagggcccc gccaacgcct
     4021 tcgccaccgg cgccttccgc gatgcggcgc tggtcgccgt gtcgagcggg ctgctc
//
LOCUS       DASPXW010000352        11646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1112092, whole genome shotgun sequence.
ACCESSION   DASPXW010000352 DASPXW010000000
VERSION     DASPXW010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11646
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112092"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..425
                     /locus_tag="VEL04_07995"
     CDS             150..425
                     /locus_tag="VEL04_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48347.1"
                     /translation="MEIRIVGSDRSSRRESEQSAWEEHSLSQLRYFRSLSLREKLEAV
                     QGMADVLRRFEEMRARGEFTSASREAARPQAAPSVHEPSPPYDDADG"
     gene            576..1229
                     /locus_tag="VEL04_08000"
     CDS             576..1229
                     /locus_tag="VEL04_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016348597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HYT48348.1"
                     /translation="MNEALVFDAYGTLFDVYSVAQRCDSFWPGKGAALSLAWRAKQLE
                     YTWQRSLMRRYRPFSEVTREALAYACEALGLQLTATQMEALMGEYLMLATYPDVVAAL
                     NKMRNKKLAILSNGSPDMLEPLVKQSGLKFDAVLSVDELQIFKPAPQVYELAATRLKL
                     SKEHIGFVSSNCWDALGAKSFGFSAYWINRAAAPVDRLGFRPDAILKTLGDLLEVLL"
     gene            1213..1746
                     /locus_tag="VEL04_08005"
     CDS             1213..1746
                     /locus_tag="VEL04_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014897161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HYT48349.1"
                     /translation="MKYCSNCASLVVKRVPPGDTLARWVCDQCGEIHYQNPRLVIGTI
                     PEYQDKILLCRRAIEPRYAYWTLPAGFMENDETAGQAALRETLEESGAQVELGAPFSM
                     ISVPHVNQVHLFYRARVLGLEFKAGEETLEVALFEEAHIPWKEIAFRTVAATLRHWFA
                     DRKSGAFGFHAEDLGLR"
     gene            1759..2454
                     /locus_tag="VEL04_08010"
     CDS             1759..2454
                     /locus_tag="VEL04_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HYT48350.1"
                     /translation="MQRRGFLKFCAAATAAGGTPVLAADARPRFYARARLLDAKGAPL
                     LARQVPAKRNLIFHYPYAATPCFLLNLGRPAKATAVLKTLDERPYEWQGGVGAERSIV
                     AYSAICAHQLTYPTRDISFISYRAEKSARNRFGNVIHCCSEHSQYDPAEGARVVAGPA
                     PQPLAAILLEHDANTDELHAVGTLGGELFNEFFEKYAFRLELEHGAAARAAVSGPCLV
                     QELDNYCRQQVKC"
     gene            2467..3156
                     /gene="aat"
                     /locus_tag="VEL04_08015"
     CDS             2467..3156
                     /gene="aat"
                     /locus_tag="VEL04_08015"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HYT48351.1"
                     /translation="MLAWLGRDDPFPPLERALKNPNGLLAAGADLSLGRLLEAYRRGV
                     FPWYSGVEPILWWSPDPRMVLYCDELKVSRSLAKNARNKGYEVRIDTVFEQVLESCAA
                     PRHGEAGTWLGSSMRKAYLALHRAGYAHAFETWRGGDLVGGLYGVALGRMFYGESMFS
                     LATDASKVALVELVCELKTRGFPLIDCQMSTPLLASLGGREIPRRSFLRELAALVDYA
                     EPPGKWSRVET"
     gene            3143..3880
                     /locus_tag="VEL04_08020"
     CDS             3143..3880
                     /locus_tag="VEL04_08020"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HYT48352.1"
                     /translation="MSKLNDLPHAVLQFYVTAPYPCSYLPDRMARSQVATPSHLINTE
                     LYGDLVKAGFRRSGIFTYRPHCDACRACVPVRIPAQEFAANRSQRRAWRQHGALAAHA
                     QPLKFRLEHYALYLRYQSKRHSGGGMDNDSRDQYSHFLLQSRVDTRLVEFRDAGQLVM
                     VSIVDFLPDGLSSVYTFYEPDRGGASFGTYNVLWQIEQCRSLGLPYLYLGYWIRESPK
                     MAYKSKFEPIEGFAGGSWRRLGGREAA"
     gene            3877..4872
                     /locus_tag="VEL04_08025"
     CDS             3877..4872
                     /locus_tag="VEL04_08025"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006400200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HYT48353.1"
                     /translation="MTAYELARPLLFALEPETAHRLGLHLARFSFSRIPACPVRTMGL
                     EFPNPVGLAAGLDKDAAHIDALARLGFGFIEVGTVTPRPQPGNPRPRLFRIIEKEALI
                     NRFGFNNVGVDAFMQNVARTRWRGVLGINIGSNADTPAERAADDYEICLEKVYARASY
                     VTLNVSSPNTQGLRNLQESDPLDALLARLSSLRKKLAERHRRLVPLVLKVAPDLDKQQ
                     IGGIARAVLRHRIDGVIATNTSVSREGVEGLPHFAEAGGLSGAPIRDRATRVLQQLHV
                     HLRNEVAIIASGGIVAQNDAAERIHAGAALVQLYTGLVYRGPRLVAECVSAFRAR"
     gene            4845..5891
                     /locus_tag="VEL04_08030"
     CDS             4845..5891
                     /locus_tag="VEL04_08030"
                     /inference="COORDINATES: protein motif:HMM:NF013430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HYT48354.1"
                     /translation="MRIGIPREIKDGEFRVALTPSGVASLKAEVILEAGAGLGVGFAD
                     HDYVKTGAKLGDPWDCELVVKVKELQEAEYRLPRRGQTVFGFQHFAPHPGLLDAALAS
                     GACFIAYETVGQDGGRLPILAPMSAIAGRLAVQAGAWCLQKHNGGSGVLLPGLEGVPA
                     GRVVILGAGNVGSNALAVAAALGATITVFAKTPRRFAALQAKYPQVKFLAGTTELESF
                     VPDADLVIGGVLTPGQMSPKLLTRDMLRRMPRGSALVDVGIDQGGIAETSRPTSHSDP
                     IYIEEGVVHYCVPNMPSACARTASLALERAVLPFIKEIVEEKYGSELETGVQVDAGRI
                     THAQLARDTRRPYR"
     gene            5888..6226
                     /locus_tag="VEL04_08035"
     CDS             5888..6226
                     /locus_tag="VEL04_08035"
                     /inference="COORDINATES: protein motif:HMM:NF026048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HYT48355.1"
                     /translation="MKRDKVALVDESRCIGCTLCIAACPVDAIVGAQSLMHTVVEPWC
                     IGCGLCLPPCPVDCIAMLPARGQWSDAHRRAAGERARARKKRLSKQKFIPETKDRRAV
                     IAAALARKRQ"
     gene            6223..6855
                     /gene="nth"
                     /locus_tag="VEL04_08040"
     CDS             6223..6855
                     /gene="nth"
                     /locus_tag="VEL04_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HYT48356.1"
                     /translation="MNPEKRREIWLRLQAANPAPTTELEYRTPFELLVAVVLSAQATD
                     KSVNKATRELFPATAEKIVSMGVAKLEKYIQSIGLYRTKAKNVVALSKIILKEHAGDV
                     PSTREALERLPGVGRKTANVVLNTAFGQPTVAVDTHIFRVANRTGLAPGKDVLEVEQK
                     LLRFTPPEFLKNAHHWLILHGRYVCLARKPGCPQCLIRDLCEFKGKTRDV"
     gene            6848..7270
                     /locus_tag="VEL04_08045"
     CDS             6848..7270
                     /locus_tag="VEL04_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008350683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HYT48357.1"
                     /translation="MFDPNREQVRDSFFGAWRKYRSGEPLVGIESLALDVILLHPEYH
                     DLLSQPERHRDKDYVDTPNPFLHMSLHVALEEQLSIDQPPGIAARFRALLARCGERHA
                     ALHEAIECLAEMVWRYQREGKPPEAAFYLECLEKRTRN"
     gene            complement(7271..7603)
                     /locus_tag="VEL04_08050"
     CDS             complement(7271..7603)
                     /locus_tag="VEL04_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HYT48358.1"
                     /translation="MRPVALAFALLAFSLSTAAEAMGNAENGKAKAAQVCAACHGPDG
                     AKPTAPDQPILAGQHYDYLVRALSDYKTGKRANPVMKVFASPLSKQEIEDLAAWFARQ
                     KSNLHFQR"
     gene            complement(7600..7953)
                     /locus_tag="VEL04_08055"
     CDS             complement(7600..7953)
                     /locus_tag="VEL04_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HYT48359.1"
                     /translation="MLALGANVALAQKKQAKKNPKPAAALAQQPAYVGDPKKAHNRIA
                     MCEGCHGIPDYKTAYPQVYHVPMLGGQQEAYIAAALHAYKSGQRSHPSMRGIAASLSD
                     EAIADLAAYYAGGLK"
     gene            8044..8889
                     /locus_tag="VEL04_08060"
     CDS             8044..8889
                     /locus_tag="VEL04_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HYT48360.1"
                     /translation="MRFKGSPSYVSTDDLTLAVNAAITLERPLLVKGEPGTGKTMLAI
                     EVARSLGLPILEWHIKSTTKAQQGLYEYDAVSRLRDSQLGDERVHDIHNYIVKGMLWQ
                     AFNAETPVVLLIDEIDKADIEFPNDLLRELDRMEFYVYETRELVKARQRPIVFITSNN
                     EKELPDAFLRRCFFHYIRFPDKETMEKIVAVHFPALKKSLLREAMEVFFEVREVPGLK
                     KKPSTSELLDWLKLLLAEDIPPEALRSKDRKTIIPPLHGALLKNEQDVHLFERLVFLT
                     RAKGA"
     gene            complement(8893..9564)
                     /locus_tag="VEL04_08065"
     CDS             complement(8893..9564)
                     /locus_tag="VEL04_08065"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HYT48361.1"
                     /translation="MAASRTPVDKRVLDAVRNFYAAGLNYRAHIEWANSRGASHKVPE
                     RADIGYRSAAALIGSGDEIVIPRDSPGPVEYEGELVAVIGRKARNISEQKALDCVAGY
                     TLGNDVSERSWQRSDRTLWRAKSADSFKPMGPFIVSGIDPMAQVIEVRVNGKTVSRYE
                     TKGMIFGVAFYIARMSRYVTLHPGDVIWMGCDAATLPALRPGDLVEVVNDAIGVLANR
                     VVRES"
     gene            complement(9564..10520)
                     /locus_tag="VEL04_08070"
     CDS             complement(9564..10520)
                     /locus_tag="VEL04_08070"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYT48362.1"
                     /translation="MRKLLFALSLFVFSVQAQEYPAKPVRMIVGFAAGGPTDVIARII
                     AQDLSISMKQSVIVENRPGANAIIATEYVARSAPDGQTLLFSSLSLLVNAILMQGRVK
                     YDPFKDFAPVSNAANLPQVVVTSPETRINSVPELIALAKSKPGEVSYATSGIGGSTHL
                     AGAMLETFTGTKMINVPFKGNGPALAEVMSGRVSFMFYPIVGIAEHVASKRLKVLAVG
                     TTSRHPDFAGVPTMAEAGLPGFEATAPWVGILAPAGTPAAVVNRLAEEMRKSLARPET
                     RERMRQLGAVIVADTPAEFAAFLRKDHERWAQVIRTSGVKAE"
     gene            complement(10517..11386)
                     /locus_tag="VEL04_08075"
     CDS             complement(10517..11386)
                     /locus_tag="VEL04_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HYT48363.1"
                     /translation="MHVTPSGKILGATIDGLDLAKPFSAPDLHAVTKALGEHGVLRFP
                     RQQLTGRQLKDFSAQLGELEINVASGAYQEPGVPEVMILSNIVENGRPIGLADAGQGW
                     HTDMSYSRMIAFANVLYGIKIPRRDGETLGATEFSSMHAAYEGLSEKTRNKLEGKTVL
                     HDFNKFWEMMRREKGSKRPPLTAAQKKAKPPVSHPVFLMHPLTGRRVLYANPGYAVRI
                     NEMSEKQSDAMLRFLFEHQLKAKYRYTFQWEEGDVLLWEDIGTIHNAVADYGPHEHRL
                     IKRCQVMANRFFQ"
     gene            complement(11388..>11646)
                     /locus_tag="VEL04_08080"
     CDS             complement(11388..>11646)
                     /locus_tag="VEL04_08080"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYT48364.1"
                     /translation="EAGLPGYLLTTWYGVFAPAGTPAAVVSRLHADLVKAMRSPGTHA
                     KLEGIGADGTVSRSPEEFAALVRADTARYAKIVKDIGLRID"
BASE COUNT         2017 a         3818 c         3773 g         2038 t
ORIGIN      
        1 tttcgattac gaatacgacc atgctctggt tggaactctc gttccgggcc tgccactgcg
       61 gttcgtgcgc atcgaaaccc ttatccggat gaaagaaacg gcgggacgcg aaaaggacaa
      121 ggaagacgtc cggcaattaa aaaagctgct tggagatcag aatcgtggga agtgatcggt
      181 catcccgccg cgaatcggag cagagcgcct gggaggagca cagcctgtcg cagctccggt
      241 atttccgctc gctgtccctg cgcgaaaagt tggaagcggt ccaaggcatg gcggacgtgc
      301 tgcgacgttt cgaggaaatg cgcgccaggg gagagttcac ctccgcttcc cgggaggctg
      361 cccggccgca agcggcaccg agcgtccatg aaccttcccc tccctatgac gatgccgatg
      421 gttagcccgg cgagcggcgc cccgggatcg cgtgtgcttg tctcgccggg ccggtcgtag
      481 gtttcgtcgg ggtccttggt gatccgcggt gaaaggccac ggccttattg aggccggatc
      541 gagtcgttcg tgaaagggaa cttatacttc ccacaatgaa cgaagccctc gtatttgacg
      601 catacggcac gctgttcgac gtttattcgg tggcgcagcg ttgcgactcg ttctggccgg
      661 ggaagggcgc tgcgctctcg ctcgcctggc gcgcgaagca gctcgagtac acctggcagc
      721 gcagcctgat gcggcgctac cgcccgtttt ccgaagtcac gcgcgaggcg ctcgcctacg
      781 cctgcgaggc gctcgggctg cagctcaccg cgacccagat ggaagcgctg atgggcgagt
      841 acctcatgct cgccacatac ccggatgtgg ttgctgcctt gaacaaaatg aggaataaaa
      901 aactcgccat tctgtcgaac ggctcgcccg acatgctcga gccgctggtg aagcaatccg
      961 gattgaagtt cgacgctgtc ctcagcgtcg atgagctgca gatcttcaag ccggcgcccc
     1021 aggtttacga gctagcggcg acgagattga agctttcaaa ggagcacatc ggcttcgtgt
     1081 cgtcgaattg ctgggacgcg ctgggcgcga agtccttcgg cttcagcgcg tactggatca
     1141 accgtgccgc ggcgccggtc gaccgcctcg ggttcaggcc cgacgcgatc ctgaagaccc
     1201 tcggagatct gcttgaagta ttgctctaat tgcgcgtccc tggtggtgaa gcgcgtgccg
     1261 cccggcgaca cgctcgcgcg ctgggtctgc gaccagtgcg gcgagatcca ctaccagaac
     1321 ccgaggctcg tcatcggcac catccctgag tatcaagaca aaattctcct gtgccggcgg
     1381 gcaatcgagc cgcgctacgc ctactggacg ctgcccgcgg gcttcatgga gaacgacgag
     1441 accgcgggcc aggcggcgct gcgcgagacg ctcgaggaat cgggcgcgca ggtcgagctc
     1501 ggcgcgccgt tcagcatgat ctcggtgccg cacgtgaacc aggtgcacct cttctaccgc
     1561 gcgcgagtgc tgggcctgga attcaaggcc ggcgaggaga cgctcgaggt ggcgctgttc
     1621 gaggaggcgc acatcccgtg gaaggagatc gcgttcagga ccgtcgccgc gaccctcagg
     1681 cactggttcg ccgaccgcaa gagcggcgcc ttcggcttcc acgccgagga cctcggcctc
     1741 aggtaaccta tagaaggaat gcagcgacgc ggctttctca agttctgcgc cgcggccacc
     1801 gcggcaggcg ggactccggt gctcgccgcc gacgcgcggc cgcgttttta tgcgcgcgcg
     1861 cggctcctcg acgcgaaagg cgcgccgctg ctcgcccggc aggtcccggc caagcgcaac
     1921 ctgatctttc actaccccta cgccgcgacg ccctgcttcc tgctcaatct cggccggccg
     1981 gcgaaggcca cggcggtcct gaaaacgctc gacgagcggc cttacgaatg gcagggcggc
     2041 gtcggcgccg agcgttcgat cgtcgcctac tcggcgatct gcgcgcacca gctcacctac
     2101 ccgacgcgcg acatcagctt catcagctat cgcgcggaaa agagcgcccg caatcgcttc
     2161 ggcaacgtga tccactgctg ctccgagcac agccagtacg accccgccga gggcgcgcgc
     2221 gtggtcgcgg gtcccgcgcc gcagccgctc gcggcgatct tgcttgagca tgatgcaaat
     2281 accgatgagc tgcatgccgt gggcacgctc ggcggcgagc tgttcaacga attcttcgag
     2341 aagtacgcct tcaggctgga gctcgagcac ggcgccgcgg cgcgcgcggc ggtcagcggc
     2401 ccctgcctgg tgcaggagct cgacaattac tgccgacaac aggtcaaatg ctgaacggca
     2461 ggtgaagtgc tagcgtggct cgggcgcgat gacccgttcc cgccgctcga acgggcgctg
     2521 aagaatccca acggcttgct cgccgccggc gccgaccttt cgctcgggcg cctgctcgag
     2581 gcctaccgcc gcggcgtgtt tccctggtac tcgggcgtcg agccgatcct gtggtggtcg
     2641 cccgacccgc gcatggtgct ctactgcgac gagctgaagg tctcgcgctc gctcgccaag
     2701 aacgcgcgaa acaagggcta tgaggtgcgc atcgacacgg ttttcgagca ggtcctcgaa
     2761 agctgtgccg caccgcggca cggcgaggcc ggaacctggc ttgggagctc gatgcggaag
     2821 gcctatcttg cgctgcaccg cgccgggtat gcccatgcat tcgagacctg gcgcggtggc
     2881 gatctggtcg gcgggctcta cggagtggcc ctcggccgga tgttttacgg agaatcgatg
     2941 ttttcgctcg ccaccgacgc ttccaaggtc gctttggtcg aactagtgtg tgaattgaag
     3001 acacggggct ttccgctcat cgactgccag atgtccacgc cgctgctcgc ttccctcggc
     3061 ggccgcgaga tcccgcggcg cagcttcctg cgcgaactgg cggcgctggt agactacgcg
     3121 gagcctcctg ggaaatggag ccgtgtcgaa acttaacgac ctgccgcatg ccgtcctgca
     3181 gttctacgtg acggcgccct atccgtgcag ctatctgccc gaccggatgg cgcgctcgca
     3241 ggtcgcgacg ccgagccatc tcatcaacac cgagctctac ggcgacctgg tcaaggccgg
     3301 gttccgccgc agcggcatct ttacctaccg gccgcattgc gacgcctgcc gcgcctgcgt
     3361 gccggtgcgc atcccggcgc aggaattcgc cgccaaccgc tcccagcggc gcgcgtggcg
     3421 ccagcacggc gcgctcgccg cccacgcgca gccgctcaag ttcaggctcg agcattacgc
     3481 gctgtacctg cgctaccagt cgaagcgcca ctccggcggc ggcatggaca acgacagccg
     3541 cgaccagtac tcgcacttcc tgctgcagag ccgcgtcgat acccgcctgg tcgaattccg
     3601 cgatgccggg cagctggtga tggtgtcgat cgtcgatttc ctgcccgacg gcctgtcgtc
     3661 ggtttacacc ttctacgagc ccgaccgcgg cggcgcgagc ttcggcacct ataacgtgct
     3721 gtggcagatc gagcaatgcc gcagcctcgg gctgccctat ctttatctcg gctactggat
     3781 ccgggagagc ccgaagatgg cgtacaagtc gaaattcgag ccgatcgagg gcttcgccgg
     3841 cggctcgtgg cgccgcctcg gggggcgcga agccgcgtga cggcctatga gctggcgcgt
     3901 ccgctcctct tcgcactaga accggaaaca gcgcatcgcc tcgggcttca tctagcaaga
     3961 ttcagttttt cccggattcc ggcctgcccg gtgcggacga tggggctgga attccccaac
     4021 cccgtgggcc tcgcggccgg cctggacaag gacgccgcgc acatcgacgc gctcgcccgt
     4081 ctcggcttcg gcttcatcga ggtcggcacg gtgacgccgc ggcctcagcc tggaaatcct
     4141 cggcccaggt tattcaggat tattgaaaaa gaagccttga tcaaccgctt cggcttcaac
     4201 aacgtcggcg tcgacgcctt catgcagaac gtcgcgcgca cccgctggcg cggcgtgctc
     4261 ggcatcaaca tcggcagcaa cgccgatacg cctgccgagc gcgcggccga cgactacgaa
     4321 atttgtctgg agaaggtgta cgcgagagcc tcttacgtca cgctgaatgt ctcttcaccg
     4381 aacacccagg ggctgcgcaa cctgcaggag agtgacccgc tggacgcctt gctcgcccgc
     4441 ctttccagtt tgagaaaaaa gctcgccgaa cgccaccgaa ggctggttcc cctggtcctg
     4501 aaggtcgcgc ccgatctcga caagcaacag atcgggggca tcgcacgagc cgtgctgcgc
     4561 catcgcatcg acggcgtcat cgccaccaac acctcggtgt cccgggaggg cgtcgaagga
     4621 ttgccgcact tcgccgaggc gggggggctt tccggggcgc cgatccgcga tcgggccacc
     4681 agggttttgc agcaattgca tgttcacctc aggaatgaag tagcgataat cgcctcgggc
     4741 ggcatcgtcg cgcagaacga cgcggcggaa aggatccacg ccggcgccgc gctggtgcag
     4801 ctctacaccg gcctcgtcta ccgcggcccg cggctggtag ccgaatgcgt atcggcattc
     4861 cgcgcgagat aaaggacggc gagttccgcg ttgcgctcac cccgtccggg gtcgcttccc
     4921 tcaaagcaga agtcattctg gaagcaggag ccggccttgg ggtcggattc gccgaccacg
     4981 actatgtaaa gaccggcgcg aagctcggcg atccttggga ctgcgagctg gtcgtcaaag
     5041 tgaaggagct gcaggaggcc gaataccggc tgccgcgccg cggccagacc gtgttcggct
     5101 tccagcactt cgccccgcat cccgggctgc tcgacgcggc gctcgcctcc ggggcgtgct
     5161 tcatcgccta cgagacggtc gggcaggacg gcggccgcct gccgatcctc gcgccgatgt
     5221 cggcgatcgc cggccggctc gcggtgcagg ccggcgcctg gtgcctgcag aagcacaacg
     5281 gcggcagcgg ggtgctgctt cccgggctgg agggcgtgcc cgcgggccgg gtcgtgatcc
     5341 tcggcgcggg caacgtcggc tcgaacgcgc tcgcggtcgc cgccgctctt ggagcgacaa
     5401 ttacggtttt cgcgaaaacg ccacggcgct ttgccgccct gcaggccaaa tatccgcaag
     5461 tgaaatttct cgccggcacg acagagctcg aaagcttcgt tcctgatgcg gatctcgtca
     5521 tcggcggcgt gctgacgccc ggccagatgt cgcccaagct gctcacccgt gacatgctga
     5581 ggcgcatgcc gcgcggctcg gcgctggtcg acgtcggcat cgaccagggc ggcatcgcag
     5641 agacttcacg gccgacctcg cacagcgacc cgatctacat cgaggagggc gtggtgcact
     5701 actgcgtgcc caacatgcct tcggcctgcg cgcgcaccgc gagcctcgcg ctcgagcgcg
     5761 cggttctgcc gttcataaaa gaaatcgtgg aagaaaaata cggctcagag ctcgaaacgg
     5821 gtgtccaggt cgatgccgga cgcatcaccc acgcgcagct cgcccgcgac acccggcggc
     5881 cttatcgatg aaacgggaca aggtcgcgct ggtcgacgaa agccgctgca tcggctgcac
     5941 gctctgcatc gccgcctgcc cggtcgacgc aatcgtgggg gcgcaatccc tgatgcacac
     6001 ggtggtcgag ccctggtgca ttggctgcgg cctgtgcctg ccgccctgcc cggtcgactg
     6061 catcgcgatg ctgccggcgc gcgggcaatg gagcgacgcc cacaggcgcg ccgcgggcga
     6121 gcgtgcacgg gcgcgcaaaa aaagactctc gaagcagaaa ttcatccctg aaacgaaaga
     6181 tcgccgcgca gtcatcgccg cggccctcgc cagaaaaagg caatgaaccc ggaaaagcgc
     6241 cgcgagatct ggctccggct gcaggcggcg aaccccgctc cgaccaccga gctcgagtac
     6301 cgcacgccct tcgagctgct ggtcgcggtg gtgctgtcgg cgcaggcgac cgacaagtcg
     6361 gtgaacaagg cgacgcggga gctgtttccg gcaacagcgg agaagatcgt gagcatgggt
     6421 gtggcaaagc tcgagaaata catccagagc atcgggctgt atcgcaccaa ggcgaagaac
     6481 gtagtcgctc tgtcgaaaat cattttgaaa gaacatgcag gagacgtgcc atccacgcgc
     6541 gaggcgctcg agcgcctgcc cggcgtcggc cgcaagaccg cgaacgtggt gttgaatacc
     6601 gcgttcggcc agcccaccgt cgcggtcgat acgcatatct tccgcgtcgc caaccgcacc
     6661 ggtctcgcgc cggggaagga tgtgcttgaa gtcgagcaga aactactccg cttcacgccg
     6721 ccggagttcc tgaagaacgc gcaccactgg ctgatcctgc acgggcgcta cgtgtgcctc
     6781 gcgcgcaagc cgggctgccc gcagtgcctg atccgcgacc tgtgcgagtt caagggaaag
     6841 actcgcgatg tttgacccga accgcgagca ggtgcgcgac agctttttcg gcgcctggcg
     6901 caagtaccgc tccggcgagc cgctggtggg catcgagtcg ctcgcgctcg acgtgatcct
     6961 gctgcacccg gaataccacg acctcctgtc gcagccggag cgtcaccgcg acaaggatta
     7021 cgtcgacacg ccgaaccctt tcctccacat gagcctgcac gtcgccctgg aggagcagct
     7081 ctcgatcgac cagcccccgg gcatcgcggc gcgctttcgc gccctgctgg cgcgttgcgg
     7141 cgagcgccac gcggcgctgc acgaggcgat cgaatgcctg gccgagatgg tctggcgcta
     7201 ccagagagaa ggcaaaccgc cggaagccgc gttctatctc gaatgtctcg agaaaagaac
     7261 ccgcaattaa ttaacgctgg aagtgcaggt tcgacttctg cctggcaaac caggccgcaa
     7321 ggtcctcgat ctcctgcttc gagagcgggc tggcaaagac cttcatcacc gggttcgccc
     7381 gcttgccggt cttgtagtcg gagagcgccc tgaccaggta gtcgtaatgc tggcccgcga
     7441 ggatcggctg gtcgggcgcg gtaggcttgg cgccgtccgg gccgtggcac gcggcgcaga
     7501 cctgcgccgc cttggccttg ccgttctcgg cgttgcccat cgcttcagct gcagttgaaa
     7561 gggaaaaagc caacagggca aaagccagtg cgacaggtct catttgagac ctcccgcgta
     7621 gtaggccgca agatccgcga tggcctcgtc ggagaggctc gccgcgatgc cgcgcatgga
     7681 cggatggctg cgctggccgg acttgtaggc gtgcagcgcg gctgcgatgt aggcttcctg
     7741 ctgcccgccg agcatcggca cgtggtagac ctggggatag gcggtcttgt agtccggaat
     7801 tccgtggcag ccctcgcaca tcgcgatcct gttgtgcgcc ttcttcgggt cgccgacgta
     7861 cgcgggttgt tgggcaagcg cggcggccgg cttcgggttc ttcttcgcct gttttttctg
     7921 agcgagcgcg acgttggcgc cgagcgcgag catgcacgcc atgccaagca ccagatcacg
     7981 caagcggttc gacattcgga attgttatcg atggaaccgg gcggatttat agcatactag
     8041 gtgatgcgct tcaaaggctc cccgtcctac gtctcgaccg atgacctgac cctcgcggtg
     8101 aacgcggcga tcacgctcga gcggccgctg ctggtgaagg gcgagccggg caccggcaag
     8161 accatgctcg cgatcgaggt ggcgcgctcg ctcggcctgc cgatcctcga gtggcacatc
     8221 aagtcgacca ccaaggcgca gcagggcctg tacgaatacg acgcggtgag ccgcctgcgc
     8281 gactcgcagc tcggcgacga gcgggtgcac gacatccaca actacatcgt caagggcatg
     8341 ctgtggcagg cgttcaacgc cgagacgccg gtggtgctgc tgatcgacga gatcgacaag
     8401 gccgacatcg agtttccgaa cgacctgctg cgcgagctcg accgcatgga gttctacgtc
     8461 tacgagacgc gcgagctggt gaaggccagg cagcggccga tcgtgttcat cacctcgaac
     8521 aacgagaagg agctgcccga cgccttcctg cggcgctgct tcttccacta catccgcttc
     8581 cccgacaagg aaaccatgga gaagatcgtc gccgtgcact tcccggcgct gaagaagtcg
     8641 ctgctgcgcg aggcgatgga ggtgttcttc gaggtgcgcg aggtaccggg gctgaagaag
     8701 aagccctcga cctcggagct gctcgactgg ctgaagctgc tgctcgccga ggacatcccg
     8761 ccggaagcgc tgcgctcgaa ggaccgcaag accatcatcc cgcccctgca cggcgcgcta
     8821 ctgaaaaacg agcaggacgt tcacctcttc gaacgtcttg tgttcttgac gcgcgccaag
     8881 ggcgcgtagt tttcagctct cgcgtaccac ccggttggcg agcacgccga tcgcgtcgtt
     8941 gaccacttcc accaggtcgc cgggccgcag cgccggcagc gtagccgcgt cgcatcccat
     9001 ccagatcacg tcgccggggt gcagggtgac atagcgcgac atgcgcgcga tgtagaacgc
     9061 gacgccgaag atcatgcctt tcgtttcgta tcgggacacc gtcttgccgt tgacgcgcac
     9121 ttcgatcacc tgcgccatcg ggtcgatgcc ggaaacgatg aacggcccca tgggcttgaa
     9181 gctgtcggcg ctcttcgcac gccagagcgt gcggtcgctg cgctgccacg agcgctcgct
     9241 gacgtcgttt cccagcgtat agccggcgac gcagtcgagc gctttttgtt cagaaatatt
     9301 ccttgctttc ctcccgatga ccgcgaccag ctcgccctcg tattcgaccg gcccgggaga
     9361 atcccgcggg atgacgatct cgtcgccgct gccgatgagc gcggctgccg agcggtagcc
     9421 aatgtcggcc cgctccggca ccttgtggct cgcgccgcgc gagttcgccc actcgatgtg
     9481 ggcgcggtag ttgaggccgg cggcgtagaa gttgcgcacc gcatcgagga ccctcttgtc
     9541 gaccggcgtg cgtgaggccg ccactactcc gccttgacgc ccgaagtccg gatcacctgc
     9601 gcccagcgct cgtgatcctt cctcaggaag gcggcgaact cggctggcgt atcggcgacg
     9661 atgaccgcgc cgagctggcg catgcgctcc cgcgtctccg gcctggcgag ggacttgcgc
     9721 atctcctcgg caagccggtt gacgaccgct gccggcgtgc ccgccggggc gaggattccg
     9781 acccacggcg cggtggcctc gaatcccggc aggcccgcct cggccatggt cggcacgccg
     9841 gcgaagtcag gatggcgcga agtcgtgccg acggcgagca ccttgagcct tttagacgcc
     9901 acgtgctcgg cgatgccgac gatcggatag aacatgaagc tgacccgccc cgacatgacc
     9961 tccgcgagcg cgggcccgtt gcccttgaaa ggcacgttga tcatcttcgt gccggtgaag
    10021 gtctcgagca tcgcgccggc gaggtgcgtc gagccgccga tgcccgaggt ggcgtagctc
    10081 acctcgcccg gcttgctctt ggcgagcgcg atgagctcgg gcacggagtt gatgcgcgtc
    10141 tccgggctgg tcaccacgac ctgcggcaag ttcgcggcgt tgctgaccgg cgcgaagtcc
    10201 ttgaacgggt cgtacttgac cctgccttgc atcaggatcg cgttcaccag cagcgacagc
    10261 gacgagaaca gcagcgtttg gccgtcgggt gccgagcgag ccacgtactc ggtggcgatg
    10321 atggcgttcg cgcctggacg gttctcgacg atcaccgatt gtttcattga aatacttaaa
    10381 tcctgggcga tgatgcgcgc aatcacatcg gtcggcccgc cggcggcgaa gccgacgatc
    10441 atgcgcacgg gcttggcggg atattcctgg gcttgaaccg aaaagacaaa aagagatagc
    10501 gcaaaaagca gctttctcat tggaagaacc gattcgccat gacctggcag cgcttgatca
    10561 gccgatgctc gtgcggaccg taatcggcga ccgcgttgtg gatcgttcca atatcctccc
    10621 acagcagcac gtcgccctcc tcccattgga aggtatagcg gtacttcgcc ttgagctggt
    10681 gctcgaacag gaaccggagc atggcgtcgc tttgcttctc gctcatctcg ttgatgcgca
    10741 ccgcgtagcc gggattggcg tatagcactc tgcggccggt gagcggatgc atgaggaaca
    10801 cggggtgcga gaccggtggt tttgccttct tctgcgccgc ggtgagcggc gggcgcttgc
    10861 tgcccttctc gcgccgcatc atctcccaga acttgttgaa gtcgtgcagc acggtcttcc
    10921 cctccagttt gtttctggtt ttttcggaga gaccttcgta cgccgcgtgc atgctgctga
    10981 actcggtggc accgagcgtc tcgccatcgc gcctggggat cttgatgcca tagagcacgt
    11041 tcgcgaacgc gatcatgcgg ctgtaggaca tgtcggtatg ccagccctga ccggcgtcgg
    11101 caagcccgat cggtctgccg ttctcgacga tgttcgagag gatcatcacc tccggcaccc
    11161 cgggctcctg ataggcgccc gaggcgacgt tgatctcgag ctcgccgagc tgcgcgctga
    11221 agtccttcag ctgccggccg gtcagctgct ggcgcggaaa ccgcagcacg ccgtgctcgc
    11281 cgagcgcctt ggtgacggcg tgcagatcag gagcggaaaa tggtttggca agatccaatc
    11341 cgtcgatggt cgcgccgagg atcttgccgc tgggcgtcac gtgcatgtca gtcgatcctg
    11401 aggccgatgt ccttcacgat cttcgcatag cgcgcggtgt ccgcgcgcac cagcgcggcg
    11461 aactcctcgg gggagcgcga caccgtgccg tccgcgccga tgccttcgag cttggcgtgc
    11521 gtcccgggcg agcgcatcgc cttcacgagg tccgcgtgca gccggctgac gaccgcggca
    11581 ggcgtgccgg cgggcgcaaa tacgccatac caggtagtga ggaggtagcc cgggagcccc
    11641 gcctcc
//
LOCUS       DASPXW010000353         5419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_478534, whole genome shotgun sequence.
ACCESSION   DASPXW010000353 DASPXW010000000
VERSION     DASPXW010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5419
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478534"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VEL04_08085"
     CDS             complement(<1..352)
                     /locus_tag="VEL04_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HYT48365.1"
                     /translation="MTELRRSAERGHANHGWLDSYHSFSFADYHDPRHMGYGVLRVIN
                     EDRVKPGSGFGTHGHRDMEIISYVLEGSLGHKDSMGNGSTIAPGDVQRMSAGRGVLHS
                     EYNHDKSGVTHFLQI"
     gene            complement(349..723)
                     /gene="trxA"
                     /locus_tag="VEL04_08090"
     CDS             complement(349..723)
                     /gene="trxA"
                     /locus_tag="VEL04_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HYT48366.1"
                     /translation="MATIDLTNENFEKTVNDNPMVVVDFWAPWCGPCRGFAPVYEQAS
                     DSHPDVVFAKVNTDEQQELAGTFGIRSIPTLMVFREKVILFQQAGALPGSALEQVISQ
                     AKSLDMAKVHEDVKQQGSKGPA"
     gene            764..1624
                     /gene="gluQRS"
                     /locus_tag="VEL04_08095"
     CDS             764..1624
                     /gene="gluQRS"
                     /locus_tag="VEL04_08095"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HYT48367.1"
                     /translation="MRTGRFAPSPTGPLHFGSLVAALASWLDARAAGGRWLVRIEDLD
                     PPREQPGAADDMLRTLERLGLYWDGEVIFQRERTTFYRKSLENLREFTYWCGCTRREV
                     ADSSLGLAADGAQIYPGTCLNGLPPGRPPRALRLKVSGSIDFIDRVQGPQRQNLENDV
                     GDFVLLRADGQFAYQLAVVVDDAEQSVTDVVRGADLLDSTARQIFLQRLLGLPTPRYL
                     HVPAAVNAAGEKLSKQTGAQPLDLSQPARGLRRALRFLGQRESTSLEEAVRNWNPALI
                     PARRTLAQVP"
     gene            complement(1587..2489)
                     /locus_tag="VEL04_08100"
     CDS             complement(1587..2489)
                     /locus_tag="VEL04_08100"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYT48368.1"
                     /translation="MSAEPDHLPRRLWWVLAGLSLAWGFNWTALKVALGEVPPWTFRS
                     LCLGLGSAVLFAALRAGGQCLAVPRGQWGRLALLALLNITCWNMLVAFGVALIPSGRA
                     AILAYTMPVWSVPLSIWLLGERVTPRKLAGLAFGVVGLGLLLGESLVSLGSAPLGTLL
                     VLGAALSWAFGTVLQKRYPVGMAAGPYTAWIMMLGGVPIFAGALLLEDLRALQSVTLW
                     PALGVAYNVFIAFAFAHWAWIKIATSVPVSIFSISMLIIPVVGVLSGMLFLGERPTWA
                     EYAALALVLGALLTVLAPGCGARG"
     gene            complement(2486..3697)
                     /locus_tag="VEL04_08105"
     CDS             complement(2486..3697)
                     /locus_tag="VEL04_08105"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HYT48369.1"
                     /translation="MRLEYRPDDPAILANPFPLYRRMQDEDPAHWSPQLKAWVLTRYD
                     DVKGACLDAGMSSDRLRPFFATLPSAEAARMEQLARTLTRWMVFRDPPDHTRLRRLAA
                     KVFHVRSIHALRPNVEALTAWLLDGIGERESFDFVSEFASPLPALVIMDMLGVPRAEL
                     ARLKHLSDEMALFIGSAREAREKYDRAEAATREMGEFFRRLITLRRSAPLPDLLSALV
                     HLEEAGDRLSDDELVATCVLLLFAGHETTAHHIANGLLALLEFPGELERLRSNPGLAA
                     AAVEELLRYDGPIGAQVRIVQAGRALHGKTLQPGERVFLMMNAANRDPRAYRDPDRLD
                     LTRDGVPHLTFGFGAHICLGFPLARLEGQIALPAVLGRWRHIELATPELRWLDSMVLR
                     GVQAMPLRVRK"
     gene            3882..4424
                     /locus_tag="VEL04_08110"
     CDS             3882..4424
                     /locus_tag="VEL04_08110"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HYT48370.1"
                     /translation="MYVTPEQIQAAGKANVETLLAVANAQFAAFEKLANINAGAVKSA
                     FEDSIANTRALLGAKDVQEFVTLQSAFAQPAIEKAIAYSKSVYEVTTEANAEFSKVAE
                     RRVAEWNENFVTLLDKVSKNAPAGSDVAVAAVKSFLAAGNAAYDNLTKVAKQATEIAE
                     ANVAAATETVKGLARAKKAA"
     gene            complement(4512..5249)
                     /locus_tag="VEL04_08115"
     CDS             complement(4512..5249)
                     /locus_tag="VEL04_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HYT48371.1"
                     /translation="MFGAFIIGDEILVGKRQDKHLAFLIEALAKRGLRLGWALYLGDD
                     APRLTAALARSFASRDVVFSCGGIGATPDDHTRQCAAAALGIGLKLHADAEREIRARF
                     GAETTPQRLAMGEFPEGASVIPNPVNRIPGFSLREHHFVPGFPQMAWPMIEWVLDTRY
                     RELFDSEKWEEASILVYEAGESQLIPAMQAVEAGFKGIKVFSLPSMGADGSRIHVELG
                     VRGDPAQVSAAMEALRRAVRKAGFPFK"
BASE COUNT          994 a         1826 c         1794 g          805 t
ORIGIN      
        1 agatctgcag gaaatgcgtc acgcccgact tgtcgtggtt gtactcgctg tgcagcacgc
       61 cgcgcccggc gctcatccgc tgcacgtcgc ccggcgcgat ggtcgagccg ttgcccatcg
      121 aatccttgtg gccgagcgag ccttcgagca catagctgat gatttccatg tcgcggtggc
      181 cgtgcgtgcc gaagccggaa ccgggcttca cccggtcctc gttgatcacg cgcagcacgc
      241 cgtagcccat gtggcgcgga tcgtgataat cggcgaacga gaagctgtgg tacgaatcga
      301 gccagccgtg attggcatga ccgcgttcgg cgctgcggcg cagctcggtc atgccgggcc
      361 cttgctgcct tgctgcttca cgtcttcatg caccttcgcc atgtcgagcg acttcgcctg
      421 gctgatgacc tgctccaggg ccgacccggg cagcgcgccc gcctgctgga acaggatcac
      481 cttctcgcgg aacaccatca gcgtgggaat cgagcggatg ccgaaagtgc cggcaagctc
      541 ctgctgctcg tcggtgttga ccttggcgaa caccacgtcg ggatgggagt cggaggcctg
      601 ctcgtagacc ggcgcgaagc cgcggcaggg gccgcaccac ggcgcccaga agtccacgac
      661 caccatcggg ttgtcgttga cggttttctc gaaattctcg ttggtcaggt cgatcgtcgc
      721 cacagcgtct ccggaaaaag cgcgcagtgt actctaggca accatgcgca caggccggtt
      781 cgcgccgtca ccgaccgggc cgctgcactt cggctcgctg gtcgcggcgc tcgccagctg
      841 gctggacgcg cgcgcggcgg gcggccgctg gctcgtgcgc atcgaggacc tcgacccgcc
      901 ccgcgagcag ccgggagcgg cggacgacat gctgcggacg ctcgagcgct tgggcctata
      961 ttgggacggg gaggtgattt tccagcgcga gcgcaccact ttctaccgga aatccctgga
     1021 aaatctacgg gaattcacgt actggtgcgg ctgcacgcgg cgcgaggtcg ccgattcctc
     1081 ccttggcctc gctgccgacg gcgcgcagat ttaccccggc acctgcctca acggcctgcc
     1141 gcccggcagg ccgccgcggg cgctgcgcct gaaggtctca gggtcgatcg atttcatcga
     1201 tcgtgttcaa ggcccccagc gccagaatct cgaaaacgat gtcggagatt tcgtgctgct
     1261 gcgcgccgac gggcagttcg cctaccagct cgcggtggtg gtcgatgacg ccgagcaaag
     1321 cgtcaccgac gtggtgcgcg gcgccgacct gctcgattcg accgcgcgac agattttcct
     1381 gcagcgcctg ctcgggcttc cgaccccgcg ctatctgcac gtgccggcgg cggtaaatgc
     1441 ggcgggcgag aaactgtcga agcagaccgg ggcgcaaccc ctcgacctgt cgcagccggc
     1501 gcgcgggttg cgccgcgcgc tgcgctttct gggccagcgc gaaagcacga gcctcgagga
     1561 ggcggtgcgc aactggaacc ctgcgcttat ccccgcgcgc cgcaccctgg cgcaagtacc
     1621 gtgaggagcg cgccgagcac cagcgcgagc gccgcgtact cggcccaggt cgggcgctct
     1681 cccaggaaca gcatgccgct cagcacgccg accaccggaa tgatcagcat gctgatcgag
     1741 aagatgctca ccggcaccga ggtggcgatc ttgatccagg cccagtgcgc gaaggcgaag
     1801 gcgatgaaca cgttgtaggc gacgccgagc gccggccaga gcgtcacgct ctgcagggcg
     1861 cgcagatcct cgagcagcag cgctccggcg aagatcggca cgccgccgag catcatgatc
     1921 caggcggtgt agggcccggc cgccatgccc accggataac gcttctgcag caccgtaccg
     1981 aaggcccagc tcagcgccgc gccgagcacc agcagggtac cgagcggagc actccccagg
     2041 ctgaccaggc tctcaccgag cagcagcccg agaccgacca cgccgaacgc caggccggcg
     2101 agcttgcgcg gcgtgacacg ctcgccgagc agccagatcg acagcggcac cgaccacacc
     2161 ggcatcgtat aggcgaggat agcggcgcgg cccgagggaa tcagcgccac gccaaacgcg
     2221 accagcatgt tccagcatgt gatgttgagt aaggcgagaa gcgcgagccg gccccactgc
     2281 cccctgggca ccgcaaggca ctggccgccc gcgcgcaacg cggcgaacag caccgccgag
     2341 ccgaggccca ggcaaagact gcgaaacgtc cacggcggca cttcgccaag cgctaccttg
     2401 agcgccgtcc agttgaagcc ccacgccagg ctgagtccgg caagcaccca ccacaggcgc
     2461 cgcggcaggt gatccggctc ggcgctcact tccggacgcg gagcggcatc gcctgcacgc
     2521 cgcgcagcac catcgagtcg agccaccgca gctcgggggt ggcgagctcg atgtgccgcc
     2581 agcgtccgag caccgccggc agcgcgatct ggccctcgag ccgcgctagg gggaacccga
     2641 ggcagatgtg cgcgccaaag ccgaaggtta ggtgcggcac gccgtcgcgc gtgagatcga
     2701 gccggtccgg atcccggtat gcgcgcggat cgcggttggc ggcgttcatc atcagaaaca
     2761 cgcgctcgcc cggctgcagc gtcttgccgt gcagcgcgcg cccggcctgc acgatgcgca
     2821 cctgggcgcc gatcggcccg tcgtagcgca gcagctcctc gacggccgca gcggcgagcc
     2881 ccgggtttga ccgcagtctt tcgagctcgc cggggaattc gagcagcgcg agcagcccgt
     2941 tggcgatgtg gtgcgcggtg gtctcgtgcc cggcgaagag cagcagcacg caggtcgcca
     3001 ccagctcgtc gtccgagagc cggtcgccgg cctcctcgag gtgcaccagc gcgctgagca
     3061 gatcgggcag cggcgcgctg cgccgcagcg tgatcaggcg gcggaagaac tcgcccatct
     3121 cgcgcgtcgc cgcctcggcg cggtcgtact tctcgcgcgc ctcgcgcgcg ctgcctatga
     3181 aaagcgccat ctcgtcggag agatgcttga ggcgcgcgag ttcggcgcgc ggcacgccga
     3241 gcatgtccat gatgacgagc gcaggcagcg ggctcgcgaa ctcggatacg aaatcgaagc
     3301 tttcgcgctc gccgatgccg tcaagcaacc aggcagtgag tgcctcgacg ttcggccgca
     3361 gggcgtggat cgagcgcacg tggaatacct tggcggcgag gcgtctcagg cgcgtatggt
     3421 ccggcgggtc gcgaaacacc atccagcgcg tgagggtgcg cgcgagctgc tccatgcggg
     3481 cggcttcggc ggaaggcagc gtggcaaaga atggccgcag ccggtcggac gacatgccgg
     3541 cgtcgaggca ggcgcctttc acgtcgtcat agcgcgtcag cacccaggcc ttgagctgag
     3601 ggctccagtg cgccgggtcc tcgtcctgca tcctgcgata aagcggaaag gggttcgcga
     3661 ggatggccgg gtcgtcgggg cggtattcaa gcctcatcga gggaaggaag cactaacttt
     3721 tcgccaataa atatgcgcgt catctgcaga agacatgatg taggcgtttg tgcgatacat
     3781 caatcactta tgctgcgacg cacaaggttg ttgactttct gtatggttgc actagaatcc
     3841 tctttgctgc accgcacaat acacacttac aggagcttta aatgtacgtt accccggaac
     3901 aaatccaagc ggccggtaag gccaatgtgg agaccctcct cgctgtcgcg aacgcgcagt
     3961 tcgccgcatt cgagaagctt gccaacatca atgccggcgc agtgaaaagc gccttcgaag
     4021 actcgatcgc gaacacgcgc gcgctgctcg gcgcgaaaga cgtccaggag ttcgtgacgc
     4081 tgcagagcgc cttcgcccag cccgccatcg agaaggcgat cgcctactcg aagagcgtgt
     4141 acgaagtgac gaccgaggcg aacgccgagt tctccaaagt ggccgagcgc cgcgtcgccg
     4201 agtggaacga gaacttcgtc accctgctcg acaaggtatc gaagaatgcg cccgccggct
     4261 ctgacgtcgc ggtggccgcg gtgaagtcct tcctcgccgc cggcaacgct gcctacgaca
     4321 acctcaccaa ggtcgcgaaa caggcgaccg agatcgccga agcgaatgtc gccgccgcca
     4381 ccgagacggt gaaagggctt gccagagcaa aaaaagccgc ctgagcgcca aaccgggcaa
     4441 gcaggccgcc cgatctagcg ccaagcacag cctcaagaag cacaagaaag cccgccgcta
     4501 ggcgggcttt tttatttgaa aggaaaaccg gccttgcgca ccgcgcgccg cagcgcttcc
     4561 atcgcggcgc tcacttgcgc agggtcgccg cgcacgccga gctcgacatg gatgcggctg
     4621 ccgtcggcgc ccatcgaagg caggctgaac accttgatgc ccttgaaccc cgcttccact
     4681 gcctgcatgg ccgggatgag ctggctctcc cccgcctcat agacaagaat cgacgcttcc
     4741 tcccactttt cggaatcgaa aagctcccgg tagcgcgtat cgagcaccca ttcgatcatc
     4801 ggccaggcca tctgcgggaa gccgggcacg aaatggtgct cgcgcagcga gaagcccgga
     4861 atgcggttga ccgggttggg aatgacgctc gcgccctcgg gaaattcgcc catcgcgagc
     4921 cgctgcgggg tggtctcggc gccgaagcgc gcgcggatct cgcgttccgc atcggcgtgc
     4981 agcttcaagc ctattcccag agcggcggcg gcgcattgcc gcgtgtgatc gtcgggcgtc
     5041 gcgccgatgc cgccgcagct gaacaccaca tcgcgcgacg caaagctgcg cgcgagcgcc
     5101 gcggtaaggc gcggcgcatc gtcgccaagg taaagcgccc agccgagccg caggccgcgc
     5161 ttcgccagcg cctcgatgag aaaagccagg tgcttgtcct ggcgcttgcc gacgagtatc
     5221 tcgtccccga tgatgaaagc gccgaacaat caatgtcccg cggcgcgcag ctcggcgagc
     5281 gcgccgagca gatagcggat gaaggcgaga ccgaagagca ccggtgcgac gaagccgagc
     5341 agcgggacgt aggcaaggag cgcgagcagc acgccgagga gacagagatg tccgcgccgc
     5401 tcgcggaaga tcagcgccc
//
LOCUS       DASPXW010000354         6018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1489183, whole genome shotgun sequence.
ACCESSION   DASPXW010000354 DASPXW010000000
VERSION     DASPXW010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6018
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1489183"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VEL04_08120"
     CDS             complement(<1..567)
                     /locus_tag="VEL04_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYT48372.1"
                     /translation="MAKVLLRDLNKKYDEIHAVKNVNLEIRDREFVVLVGPSGCGKTT
                     TLRMVAGLEEISSGEISIDGRVVNDLAPMDRDIAMVFQNYALYPHMSVYDNMAFGLRM
                     RKFDKPEIERRVRRSAEILGIQALLERKPRQLSGGQRQRVALGRAIVRNPRVFLFDEP
                     LSNLDAKLRGEMRREIARIHRDARATSLY"
     gene            complement(592..1443)
                     /locus_tag="VEL04_08125"
     CDS             complement(592..1443)
                     /locus_tag="VEL04_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HYT48373.1"
                     /translation="MIEGARWKKWLWFYLPMGMFILGLLFPFYWMLVTTVRPDGELYR
                     PWNHPLYMPFWTTQPTLDHIKNLLQETLFNQWMWNTMLIAFAATLISLVCGVLAGYAL
                     ARLRFRYAGLLGTAIFITYLVPQTLLFIPLADIIREYRIGDTPWALILTYPTFLIPFC
                     TWLMMGYFKTIPKELEECARIDGATRWQAMLYIVFPIAVPGILSAGIFAFTLSWNEFI
                     YALVFLSSPEQKTVPVGVVSELIRGDVFYWGPLMAGALIGSIPVAIVYSFFVEYYVTG
                     MTGSVKG"
     gene            complement(1440..2312)
                     /locus_tag="VEL04_08130"
     CDS             complement(1440..2312)
                     /locus_tag="VEL04_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HYT48374.1"
                     /translation="MLEREGALAFWLLVPTLALLTLFIAYPFVRGVWLSLTNAVVGVP
                     GDFVGLKTFAKILDDSIFRTAALNTFVYTAVTTVFKLALGLWLALLLNRHFRGKAWVR
                     AFILLPFIIPTVLSTLAWKWMFDPTFSVFNWLLWKLSIINSRINWLGDPELAMASIMT
                     VNVWRGVPFYAISLLAGLQTINPDLHEAAAIDGANRWQRFWHVTWPLLLPVTMVVVLF
                     SIIQTFADFQLVYVLTGGGPANATHLFATYAYQIGIGTGLLSEGAAISLAMFPALLLV
                     VVFQLWYIRRVENR"
     gene            complement(2341..3645)
                     /locus_tag="VEL04_08135"
     CDS             complement(2341..3645)
                     /locus_tag="VEL04_08135"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYT48375.1"
                     /translation="MAQFDRRKFLQYSGAGLAGILASHQAPVFAQAGTVHWVKWNDFV
                     PASDELLRKTILPEASKALGLKINLETINGNDLQPRITSAIQSGAGPDVFMLFNNHPH
                     LYAASVANVSDVTEAISKAQGGYYRLCRNNTSDGKKWLSVPWCVIGAQIAYRKSWFEE
                     VGYSKFPETWEQYRDAGKKLKAKGRPIGQTLGHTFGDSPTFTYPFLWSYGGKEVDEKG
                     KVVLDSKATVESVKFMQAFWKDAHDEGGLAWDDSNNNRAFLSGTICATLNGASIYIES
                     LRKADQYKTEKGQQLKTDILHAALPGGPAGQFAMHTFQSHVVPNYSKNQKAAKEFLKW
                     MHTQKNYDQWFVSQKGFATAPTQVWEKHKMWDEDPVMAPYRVAARLGLTPGYAGPANR
                     KAAEVLTKYIITDMYAKAVQGMTPEAAVKWAAEEVSKVYAGT"
     gene            complement(3680..4960)
                     /locus_tag="VEL04_08140"
     CDS             complement(3680..4960)
                     /locus_tag="VEL04_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HYT48376.1"
                     /translation="MDILVLLGSFTLLCALAVPVAYALGLAALIAALYVDIPLEAVML
                     KISDGVDDFALLAIPFFVLAGAIMAEGGMAARLVNLAKVFVGFIRGGMAIVNILASTL
                     FGCISGSSVADTASIGSVMIPQMIRQGYPRVFATNVTICGSVQALMIPPSHNAVIYSV
                     AAGGTISVASLFLAGILPGLLFGACLIGLVVWTAHKRSFPVSEPTPLKDVPRIVCEAL
                     WGLVTVFIIMGGILSGVFTPTESAAVACIYAFLVTFLVYRDYKWRDLPHLVHRVIKTV
                     AMVMMLIGFSVGFGYMMAIMQIPAKITLFFLSISENKYVFLLLVNIVLLLLGTFMDLA
                     PMLLICTPIFLPVIYKLGIDPVHFGMIMILNLGIGLITPPVGPTLFVGCAIGKVTMEE
                     VSKELWPFYGAMCLALLLVTYIPAISLWIPGLMK"
     gene            complement(4993..5487)
                     /locus_tag="VEL04_08145"
     CDS             complement(4993..5487)
                     /locus_tag="VEL04_08145"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HYT48377.1"
                     /translation="MQQLYLACIVLSGTALVVITLIIPLGVFMRYARNDPLSWPEPAA
                     VVMMVMFSFIGGAAVYRANAQIAVEALIKAVGPKTRTAMTWGVHACLAITAAFMLGYG
                     IHLCELTWYNTMAEFPELRVGIVYMPIPIGGLLTLLFLIEKVWIGDPPKTDVMYSDQA
                     AELE"
     gene            complement(5567..>6018)
                     /gene="dctP"
                     /locus_tag="VEL04_08150"
     CDS             complement(5567..>6018)
                     /gene="dctP"
                     /locus_tag="VEL04_08150"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HYT48378.1"
                     /translation="LFVETMNAMGGNGVAMGFNELYSALQTGVVDGAENNPPTLLAQN
                     HYQVSKVYSLTGHLIIPEIFVFSKRNWETLPKADQDLVKKLSREAQLEQRKLWDAYVG
                     EAEGKLKAAGIQFVKADKQAFYKATQPVRDKYGAKYAALLKKIEDTK"
BASE COUNT         1133 a         1856 c         1974 g         1055 t
ORIGIN      
        1 gtagagcgag gtggcgcggg cgtcgcggtg gatgcgcgcg atctcgcgcc gcatctcgcc
       61 gcgcagcttg gcgtcgaggt tcgagagcgg ctcgtcgaac aggaacaccc gcgggttgcg
      121 cacgatggcg cggccgagcg cgacgcgctg gcgctgtccg ccggagagct ggcgcggctt
      181 gcgctcgagc agcgcctgga tgccgaggat ctcggctgag cgccgcacgc ggcgctcgat
      241 ttcgggcttg tcgaacttgc gcatcctgag gccgaacgcc atgttgtcgt acacgctcat
      301 gtgcgggtac agggcgtagt tctggaacac catcgcgatg tcgcggtcca tcggcgcgag
      361 atcgttcacc acgcggccgt cgatgctgat ctcgcccgag ctgatctcct ccagcccggc
      421 gaccatgcga agcgtggtgg tcttgccgca gcccgacggt ccgaccagca ccacgaactc
      481 gcggtcgcgg atctcgaggt tcacgttctt cacggcgtga atctcgtcgt acttcttgtt
      541 cagatccctg agaagaactt tcgccatgca acctgccctt taccctgccg tttaaccttt
      601 gacgcttccg gtcatgccgg tgacgtagta ctcgacgaaa aatgaataga cgatcgccac
      661 cgggatcgag ccgatcaggg cgccggccat cagcggcccc cagtagaaca cgtcgccgcg
      721 gatcagctcg gagaccacgc cgaccggcac cgtcttctgc tcgggcgagg agaggaacac
      781 cagcgcgtag atgaactcgt tccacgacag cgtgaaggcg aagatgccgg ccgagaggat
      841 gcccggcacc gcgatcggaa agacgatgta cagcatcgcc tgccagcgcg tcgcaccgtc
      901 gatgcgcgcg cattcctcga gctccttcgg gatggtcttg aagtagccca tcatcagcca
      961 ggtgcagaac gggatcagga aggtggggta ggtaaggatc agcgcccagg gcgtgtcgcc
     1021 gatgcggtac tcgcggatga tgtcggcgag cgggatgaag agcagcgtct gcggcaccag
     1081 gtaggtgatg aagatcgcgg tgcccagcag tcccgcgtag cggaatcgca ggcgcgcgag
     1141 cgcatagccg gccagcaccc cgcagacgag cgagatcagc gtcgcggcga acgcgatcag
     1201 catggtgttc cacatccact ggttgaacag cgtctcctgc agcaggttct tgatgtggtc
     1261 gagggtgggc tgagtggtcc agaacggcat gtagagcgga tggttccagg gccggtagag
     1321 ctcgccgtcg ggccgcaccg tggtcaccag catccagtag aacgggaaca gcaggccgag
     1381 gatgaacatc cccatcggca ggtagaacca gagccacttc ttccagcgtg cgccttcgat
     1441 catcggttct ccacccgccg gatgtaccag agctggaata ccaccaccag aagcagcgcc
     1501 gggaacatcg ccagcgagat cgcggcgcct tcggagagca gcccggtgcc gatgccgatc
     1561 tgataggcgt aggtggcgaa gaggtgcgtc gcgttggccg ggccgccgcc ggtcagcacg
     1621 tagaccagct ggaagtcggc gaaagtctgg atgatcgaga acagcaccac caccatggtg
     1681 accggcagca gcagcggcca ggtgacgtgc cagaaccgct gccagcggtt ggcgccgtcg
     1741 atggcggcgg cttcgtgcag gtcgggattg atggtctgca gccccgcgag caggctgatg
     1801 gcgtagaacg gcacgccgcg ccacacgttc accgtcatga tcgaggccat cgccagctcc
     1861 gggtcgccca gccagttgat gcgcgagttg atgatggaga gcttccagag gagccagttg
     1921 aagacgctga aggtggggtc gaacatccat ttccaggcga gcgtcgagag caccgtcggg
     1981 atgatgaatg gcagcaggat gaaggcgcgc acccaggcct tgccgcggaa atggcggttg
     2041 agcagcagcg cgagccacag cccgagcgcc agcttgaaca ccgtggtgac cgcggtatag
     2101 acgaaggtgt tgagcgcggc ggtgcggaag atcgagtcgt ccaggatctt ggcgaaggtc
     2161 ttcagaccga cgaaatcgcc cggcacgccg acgacggcgt tggtcagcga gagccacacg
     2221 ccgcgcacga acggataggc gatgaacagc gtgagcagcg caagcgtggg cacgagcagc
     2281 cagaaggcga gcgcgccctc gcgctcgagc atccggctgc tccttgcggc gggcaaggcg
     2341 ttacgtgccc gcgtagacct tcgagacctc ctcggccgcc cactttacgg cggcttcggg
     2401 cgtcatgccc tgcaccgcct tcgcgtacat gtcggtgatg atgtacttgg tgagcacttc
     2461 ggcagccttg cggttcgccg gcccggcata gcctggcgtg agcccgaggc gcgctgcgac
     2521 ccggtagggc gccatcaccg ggtcctcatc ccacatcttg tgcttctccc acacctgcgt
     2581 cggcgcggtg gcgaagcctt tctgcgagac gaaccactgg tcgtagttct tctgagtatg
     2641 catccacttg aggaattcct tcgccgcctt ctggttcttc gagtagttcg gcacgacgtg
     2701 cgactggaag gtgtgcatcg cgaactgtcc ggcggggcct ccgggcagcg ccgcgtgcag
     2761 gatgtcggtc ttcagctgct gccccttctc cgtcttgtac tggtcggcct tgcgcagtga
     2821 ttcgatgtag atcgaagcgc cgttcagcgt ggcgcagatc gtgcccgaca ggaaggcgcg
     2881 gttattgttc gagtcgtccc aggcgaggcc gccctcgtcg tgcgcgtcct tccagaacgc
     2941 ctgcatgaac ttgaccgact ccacggtggc tttcgagtcg agcaccactt tgcctttctc
     3001 gtcgacctcc ttgccgccgt aggaccagag gaacggatag gtgaacgtgg gcgagtcgcc
     3061 gaaggtgtgg ccgagcgtct ggccgatggg ccggcccttg gccttcagct tcttgccggc
     3121 gtcgcggtac tgctcccagg tctcggggaa tttgctgtag ccgacctcct cgaaccacga
     3181 cttgcggtag gcgatctgcg cgccgatcac gcaccacggc accgacagcc atttcttgcc
     3241 gtcgctcgtg ttgttgcggc acaggcggta gtaaccgccc tgagccttgc tgatcgcctc
     3301 ggtcacatcg ctcacgttcg cgacgctcgc cgcgtacagg tgcggatgat tgttgaacag
     3361 catgaagacg tccggccccg cccccgactg gatcgccgag gtgatgcgcg gctgcagatc
     3421 gttgccgttg atcgtctcga ggttgatctt gaggccgagc gccttgctcg cttcgggaag
     3481 aatggtcttg cgcagcagct cgtccgatgc cgggacgaag tcgttccact tcacccagtg
     3541 caccgttccg gcctgcgcga acaccggcgc ctggtgcgag gcgaggatgc cggcgagacc
     3601 ggctcccgaa tactgcaaga atttgcgtcg atcgaactga gccatgtcgt ctcctcctcc
     3661 ttgaaggccc accactttac tacttcatca ggccgggaat ccagagcgaa atggcgggaa
     3721 tgtaggtgac gagcagcagc gcgaggcaca tcgcgccgta gaacggccac agctccttcg
     3781 acacctcctc catggtcact ttgccgatgg cgcagccgac aaaaagggta ggacccacgg
     3841 gaggtgtgat caggccgatg ccgagattca ggatcatgat catgccgaag tgcaccgggt
     3901 cgatgccgag cttgtagatc accggcagga agatcggcgt gcagatgagc agcatcggcg
     3961 cgagatccat gaaggtgccg aggagcagca gcacgatgtt caccagcagc aggaagacgt
     4021 acttgttttc cgagatgctg aggaagaaca gcgtgatctt cgccggaatc tgcatgatcg
     4081 ccatcatgta gccgaagccg acggaaaaac cgatcagcat catgaccatc gccacggtct
     4141 tgatgacgcg atgcacgagg tgcggcagat cgcgccactt gtaatcgcga tacacgagga
     4201 aggtcacgag gaaggcgtag atgcacgcca ccgccgccga ttccgtcggg gtgaacacgc
     4261 ctgaaagaat tccgcccatg atgatgaaga cggtcaccag cccccacagc gcctcgcaca
     4321 cgatcctcgg cacatctttg agcggggtgg gctcggatac cggaaagctg cgcttgtgcg
     4381 cggtccacac gaccaggccg atcaggcagg cgccgaacag gagccccggc aggatgccgg
     4441 cgaggaacag gctagcgacc gagatggtgc cgccggcggc gacggagtag atcaccgcgt
     4501 tgtgtgaagg cgggatcatc agcgcttgca ccgagccgca gatcgtgacg ttagtggcga
     4561 agacgcgcgg atagccctgc ctgatcatct gcgggatcat caccgagccg atcgaggcgg
     4621 tatcggcgac cgacgagccc gagatgcagc cgaagagcgt cgaggcgagg atgttgacga
     4681 tcgccatgcc gccgcggatg aagccgacga acaccttggc gaggttcacc aggcgcgccg
     4741 ccatgccgcc ttccgccatg atcgcccccg ccagcacgaa gaacggaatg gcgagcagcg
     4801 cgaagtcgtc gacgccatcc gagatcttga gcatcaccgc ttcgaggggg atgtcgacgt
     4861 agagcgcggc gatgagcgcg gcaagcccga gcgcgtaggc aaccggcacg gcaagcgcgc
     4921 acagcagcgt gaaagacccg aggagaacga gaatgtccat ctagggtgcg ggagacgagt
     4981 ctcctgctcc tttcactcga gctccgcggc ctggtcgctg tacatgacgt cggtcttcgg
     5041 cgggtcgccg atccagactt tctcaatgag gaacagcagc gtcaggaggc cgccgatcgg
     5101 gatcggcatg tagacgatac ccacgcgcag ctccgggaac tccgccatcg tgttgtacca
     5161 ggtgagctcg cagaggtgga tgccgtagcc caacatgaaa gcggcggtga tcgctaagca
     5221 ggcgtgtacg ccccaggtca ttgcggtccg ggttttcggg ccgaccgcct tgatcagcgc
     5281 ttcgaccgcg atctgcgcgt tggcgcggta gaccgccgcc ccgccgatga acgagaacat
     5341 caccatcatg accaccgccg ccggctcggg ccacgagaga gggtcgttcc tggcatagcg
     5401 catgaaaacg ccgagcggga tgatcagcgt gatcaccacc agcgccgtgc cggacagcac
     5461 gatgcacgcc aggtaaagct gctgcatcgc cttttgatag ccttgcttca tacaaaaacg
     5521 ggccgccgtt tctcgtcggg cggcccgctt ctccttccgg gaaggcttac ttcgtgtcct
     5581 cgatcttctt cagcagagcg gcgtacttgg cgccgtattt gtcgcgcacc ggctgcgtgg
     5641 ctttgtagaa ggcctgcttg tcggccttga cgaactggat cccggcggcc ttgagcttgc
     5701 cctcggcttc gccgacatac gcgtcccaca gcttgcgctg ctcgagctgc gcctcgcgcg
     5761 agagcttctt gaccaggtcc tgatcggcct tgggcagcgt ctcccagttg cgctttgaga
     5821 agacgaagat ctcgggaatg atcaggtggc cggtgaggct gtagaccttg ctcacctgat
     5881 agtggttctg ggcgagcagc gtcggcgggt tgttctccgc gccgtccacc acgccggtct
     5941 gcagggcgga gtagagctcg ttgaagccca tcgcgacgcc gttgccgccc atcgcgttca
     6001 tggtttccac gaacagcg
//
LOCUS       DASPXW010000355         3386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_105018, whole genome shotgun sequence.
ACCESSION   DASPXW010000355 DASPXW010000000
VERSION     DASPXW010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105018"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..562)
                     /locus_tag="VEL04_08155"
     CDS             complement(128..562)
                     /locus_tag="VEL04_08155"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF016267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HYT48379.1"
                     /translation="MTSIDFYFNAADRLQVACRLAGKSVAQGKRMLIYAPQPEVASHI
                     DKLLWTWPAIGFVPHCAAHDAAAPETPVLIAAAQETPPGCELLLNLSADCPPHFERFE
                     RLLEIVSAHDAERKAGRERYRFYLERGYRISDHDLAKDGERE"
     gene            complement(559..2037)
                     /locus_tag="VEL04_08160"
     CDS             complement(559..2037)
                     /locus_tag="VEL04_08160"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HYT48380.1"
                     /translation="MEFSIKALSPEAAKAGCVVVGIHSGKELTPAARRVDQAARGALR
                     AALGDVSGKTGSTLLLRGLPGVAAERVLLVGLGERREFAEAQFRDAVRAAANALKELG
                     AKDAALFLVDAKVGARAPGWNVRHAVLGMREAFYRFDQLKTQKKAPQPALRQVFLPLS
                     SNSKLQDALAEASATADGTALARTLGNLPANICTPAYLAAEAAKLARQFKLGIEVLER
                     RDMEKLGMGALLSVARASHQPAKLIVLRYAGGAKAKKPLVLVGKGITFDTGGISLKPA
                     GEMDEMKFDMSGAASVLGTLKALAAMRAPVSVVGVMPACENMPGGAASKPGDVVTTLS
                     GQSVEILNTDAEGRLILCDALAYAARFNPEVVVDIATLTGACVVALGHVASGLFANDQ
                     KLADELREAADDACDRVWQMPLWEDYQEQLRSNFADMANIGGRPAGSITAACFLARFT
                     RKLRWAHLDIAGTAWKSGREKGSTGRPVPLLVRFALRHAERK"
     gene            2052..3128
                     /gene="lptF"
                     /locus_tag="VEL04_08165"
     CDS             2052..3128
                     /gene="lptF"
                     /locus_tag="VEL04_08165"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HYT48381.1"
                     /translation="MIFRRALLREFTGLAGAVFMTLFAIALTTQLIRLLGKAAGGQVP
                     SDAVIAFLGFFALGALPVLLSLTMFISVLLTLTRGWRDSEMVIWFGSGLSLTAWLKPV
                     MLFALPQIVLIAALSLFISPWAAQMAGQYGKRLESRDDVTRISPGVFGETANKERVYF
                     VESVSDDSNTVQNVFVASVHQNRTGVSMSRSGHTETAPNGDRFIVLEKGRRYEGVPGD
                     ADYRITEFDRYAARIETKEGAEPEATPKSLTSWSLIANPTNVNLAELMWRVGIPVSAL
                     VLALLAIPMSFVNPRAGRSANLLFALFTFMVYSNLLSISQARVAQGRLDFAIGCWLVH
                     AGMLVLLVAMFAQRMQLIRLRLGR"
     gene            3138..>3386
                     /locus_tag="VEL04_08170"
     CDS             3138..>3386
                     /locus_tag="VEL04_08170"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HYT48382.1"
                     /translation="MQPLQTIERYLGRQIYAAVGFVLLGFLALFAFFDLIKELADLGR
                     GDYHLRQVFTFVALSIPAHAYELFPVVVLIGTLYVLAHL"
BASE COUNT          476 a         1200 c         1128 g          582 t
ORIGIN      
        1 cgttcgagct cgcgcgcgag cgcctcgagc gcggccgcgt cgaccgcgcg tcgcggcgcg
       61 cgcgagtcat tgacggtttc ggtgagcacc gggaccaccc ggtcggggtt gcggctgtct
      121 tgctcgctca ctcgcgctcg ccgtccttcg ccaggtcgtg atcgctgatg cggtagccgc
      181 gctcgaggta gaagcgatag cgctcgcggc cggctttgcg ctccgcgtcg tgcgccgaaa
      241 cgatttcgag caggcgctcg aagcgctcga agtgcggcgg gcagtccgcc gacagattca
      301 gcagcagctc gcagcccggc ggcgtctcct gcgccgcggc gatcagcacc ggcgtctcag
      361 gcgcagccgc gtcatgcgcg gcgcaatgcg gcacgaagcc gatcgccggc caggtccaca
      421 gcagcttgtc gatgtggctc gccacctcgg gctgcggcgc atagatcagc atgcgcttgc
      481 cctgcgccac ggacttgccg gccagccggc aggccacctg cagccgatcc gcggcgttga
      541 aatagaaatc gatgctggtc atttcctttc cgcgtggcgc agcgcgaagc gcaccagcag
      601 cggcaccggc cgcccggtgg agcctttctc tcgcccgctt ttccacgccg tgcccgcgat
      661 gtccaggtgc gcccagcgca gcttgcgcgt gaagcgggcg aggaagcacg ccgcggtgat
      721 gctgccggcg ggccgcccgc cgatgttcgc catgtcggcg aaattggagc gcagctgctc
      781 ctggtaatcc tcccacagcg gcatctgcca cacccggtcg caggcgtcgt cggcggcctc
      841 gcgcagctcg tcggccagct tctggtcgtt ggcgaacagc ccgctcgcga cgtggcccag
      901 ggcgacgacg caggctcccg tgagagtggc gatatcgacc accacctcgg gattgaagcg
      961 cgccgcgtag gcgagggcgt cgcacaggat cagccgcccc tcggcgtcgg tgttgaggat
     1021 ctcgacgctc tgccccgaga gggtagtcac gacgtcgccg ggtttcgacg cggcgccgcc
     1081 cggcatgttc tcgcacgccg gcatcacgcc cacgacgctg accggggcgc gcatcgcggc
     1141 gagcgccttc agcgtgccga gcacgctcgc cgcgcccgac atgtcgaact tcatctcgtc
     1201 catctcgccg gcaggcttga gcgagattcc gccggtatcg aaggtgatgc ccttgcctac
     1261 gagcacgagc ggtttcttcg ccttcgcgcc gcccgcatag cgcagcacga tcagcttcgc
     1321 aggctggtgc gaggcccgcg ccaccgagag cagcgcgccc atgccgagct tttccatgtc
     1381 gcgccgctcg agcacctcga tgccgagctt gaactggcgc gcgagcttcg ctgcctcggc
     1441 ggcgaggtag gccggcgtgc agatattggc gggcaggttg ccgagcgtgc gcgcgagcgc
     1501 ggtgccgtcg gcggtggcgc tcgcctccgc cagcgcgtcc tgcagcttgg aatttgagct
     1561 gagaggcaaa aaaacctgac gcagcgcagg ctgcggggcc ttcttctgcg tcttgagctg
     1621 gtcgaaacgg tagaacgcct cccgcatgcc gagcaccgcg tgccgcacgt tccagcccgg
     1681 cgctcgcgcg cccaccttgg catcgaccag gaacagtgcc gcgtccttcg ctcccagctc
     1741 cttgagcgcg ttcgccgccg cgcgcaccgc gtcgcgaaac tgcgcctctg caaattccct
     1801 gcgctctccc aggccgacca gcagcacccg ctcggcggcg acgcccggca ggccgcgcag
     1861 cagcagcgtc gagccggtct tgcccgacac atcgccgagc gccgcgcgca gcgcgccgcg
     1921 cgccgcctgg tcgacgcgcc gcgccgctgg cgtaagctct ttcccggaat gaatgccgac
     1981 cacgacgcag cccgctttcg ccgcctcggg cgagagcgcc tttatgctaa attccatcgc
     2041 gaaagtatcg catgatcttc cgacgcgcgc tgctcaggga attcaccggt ctcgccggcg
     2101 cggtgttcat gacgctgttc gcgatcgcgc tcaccacgca gctgatccgc ctgctcggca
     2161 aggcggccgg cggccaggtg ccgtccgatg ccgtgatcgc gttcctcggc ttcttcgcgc
     2221 tcggcgcgct gccggtgctg ctgtcgctca ccatgttcat ctcggtgctg ctcacgctga
     2281 cgcgcggctg gcgcgattcc gagatggtga tctggttcgg ctccggactg tcgctcaccg
     2341 cctggctcaa gccggtgatg ctgttcgcgc tgccgcagat cgtgctgatc gcggcgctgt
     2401 cgctgttcat ctcgccgtgg gcggcacaga tggcggggca gtacggcaag cgcctcgagt
     2461 cgcgcgacga tgtcacgcgg atcagtcccg gcgtgttcgg cgagaccgcc aacaaggagc
     2521 gcgtgtactt cgtcgaatcg gtctcggacg actcgaacac cgtgcagaac gtgttcgtgg
     2581 cctcggtgca ccagaaccgc accggggtca gcatgagccg ctccgggcac accgagaccg
     2641 cgccgaacgg cgaccgcttc atcgtgctcg agaagggcag gcgctacgag ggcgtgccgg
     2701 gcgacgccga ctatcgcatc accgagttcg accgctacgc ggcgcgcatc gagaccaagg
     2761 agggcgcgga gcccgaggcc acgccgaaga gcctgaccag ctggtcgctg atcgccaatc
     2821 cgaccaacgt caacctcgcg gagctgatgt ggcgggtggg catcccggtt tcggcgctgg
     2881 tgctggcgct gctcgccatt cccatgagct tcgtcaatcc gcgcgccggc cgctccgcga
     2941 acctgctgtt cgcgctgttc accttcatgg tgtactcgaa tctcctcagc atcagccagg
     3001 cgcgcgtggc gcagggcagg ctcgacttcg ccattggctg ctggctggtg catgccggca
     3061 tgctggtgct gctggtggcg atgttcgccc agcgcatgca gctgatccgc ctgcgcctgg
     3121 ggcgttgagg acccggcatg cagccgttac agacgataga gcgctacctc gggcggcaga
     3181 tctatgccgc cgtcggcttc gtgctgctcg gctttctcgc gctgttcgcg ttcttcgacc
     3241 tgatcaagga gctcgcggac cttggcaggg gcgactacca cctgcggcag gtgttcacct
     3301 tcgtcgcgct ctcgatcccg gcgcatgcct acgagctgtt cccggtggtg gtgctcatcg
     3361 gcacgctgta cgtcctcgcg cacctc
//
LOCUS       DASPXW010000356         3510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1865810, whole genome shotgun sequence.
ACCESSION   DASPXW010000356 DASPXW010000000
VERSION     DASPXW010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1865810"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..1036
                     /locus_tag="VEL04_08175"
     CDS             152..1036
                     /locus_tag="VEL04_08175"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYT48383.1"
                     /translation="MTTLGQLVFSGILIGGIYALMSIGLTLIFGVLRIVNFAHGEFLM
                     LGMYGAWALSTMAGLNPYTAALAIVPAMFLFGGLVHRIIISPALDKPHLVVVFATMGL
                     SIFMQNVALMMMTADLWDVPPVFSRAVTIGPFFFKPELLLGFAITIACTLALQWMIRA
                     TYLGKAIRATVQDGEAAMLMGIPVPRIFLITFAGGSALVGLSACVMMPLFSVFPTVGL
                     NFVLIAFVIVVLGGMGSIEGALIGGICIGVVQSLSSYYVAPAFGQMFFFILFLLVMIL
                     RPNGILGQKGAATIGIND"
     gene            1045..2067
                     /locus_tag="VEL04_08180"
     CDS             1045..2067
                     /locus_tag="VEL04_08180"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYT48384.1"
                     /translation="MSIAALDPAPRKSRRLPALPQRGAVSWFEIALLTACLVLVAVEL
                     PQNLRDVIVLTFLFGALALAWNIAGGYAGLISFGHAAFFGIGAYTSTILFVHGGLSPW
                     IGLWLGALLSAAAGAFLAVICARLRGPFFILSTLACAEVVRIGALNWASLTGGPEGLS
                     ILPVASLANMVFASKTVYAALMLAYLVICYAATKALECSRYGYYLFAVRDDEEAAGAA
                     GVNPLLARAGAMALSAALTGIGGSLFAQYFLYLDPTFVISPDLSFQLALLPAVGGLGT
                     AIGPVLGSFLITPLSELLRAYLGNAAAGLHLVIYSAGLIVAMLYFPSGLAGALSRLAG
                     GGKGRP"
     gene            2064..2792
                     /locus_tag="VEL04_08185"
     CDS             2064..2792
                     /locus_tag="VEL04_08185"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT48385.1"
                     /translation="MTGILEVRNVSRAFDALWAVREVSFSVAPGELLGLIGPNGAGKS
                     TLYNLIAGGLKPTHGEIVFAGRRVTGWRSYQVARIGLARTFQIPKPYRQLSVTENVML
                     TAFQHEPSLARSRALADATLADVGLSDYAAAPIATLTVGLLKRLEVARALAMRPKLVL
                     FDEIMAGLTPTEVGFMTRLVAGLPARGITVLWVEHVLYAIMKTASRIMVLNRGEVIAD
                     GAPAAVARDPVVVKAYLGEEMSLA"
     gene            2785..3489
                     /locus_tag="VEL04_08190"
     CDS             2785..3489
                     /locus_tag="VEL04_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT48386.1"
                     /translation="MLEVRHLNAFYGPVQVLHDVSLSVPVGQIVALVGANAAGKSTLM
                     FTLAGLRTTHTGELVFDGEPLGALSAYERVPRGLVLVPERRRLFPFMTVLENLEIGAY
                     SAAARPAARATLQQVFALLPVLDERRKQVAGSLSGGEQQMLAIGRALMAKPRMLLLDE
                     PTEGLAPIYVRRLFDLIGNLRSTGLTVLIVEQNVHHVLRMADRGYVLENGRIVLEGRG
                     EDLLNDERLKVAYLGL"
BASE COUNT          492 a         1181 c         1153 g          684 t
ORIGIN      
        1 tctgacacag taggattgga tcaattgctg tccgggttac ggtccctcaa ttcttcgccc
       61 cctcacccaa ccctctcccc gacggccccg acggggagag ggagatcgcc gagttcgcgg
      121 ctgaacaaat gcatgaaacc gaagcgttga aatgaccacg ctcggacagc tcgtcttttc
      181 cggcatcctc atcggcggca tctacgcgtt gatgagcatc ggcctgacgc tgatcttcgg
      241 cgtcctgcgc atcgtcaatt tcgcccacgg cgagttcctg atgctcggca tgtacggcgc
      301 gtgggcgctt tcgaccatgg cagggctcaa cccgtacacg gcggcgcttg cgatcgtgcc
      361 ggcgatgttc ctgttcggcg gcctggtgca ccggatcatc atcagcccgg cgctcgacaa
      421 gccgcatctc gtcgtcgtgt tcgccaccat gggcctgtcg atcttcatgc agaacgtggc
      481 gctgatgatg atgacggcgg acctctggga cgtgccgccg gtcttcagcc gcgccgtcac
      541 gatcggcccg ttcttcttca agcccgagct tctgctcggc ttcgccatca ccattgcgtg
      601 cacattggcg ctgcaatgga tgatcagggc gacctatctg ggcaaggcga tccgcgcgac
      661 cgtgcaggac ggcgaggccg ccatgctcat gggcatcccg gtgccgcgca tcttcctcat
      721 cacgtttgcc ggcggctcgg cgctggtcgg cctgtccgcc tgcgtgatga tgccgctgtt
      781 ttccgtgttc cccacggtgg gcctcaactt cgtgctgatc gcgttcgtca tcgtcgtgct
      841 cggcggcatg ggcagcatcg agggcgcgct gatcggcggc atctgcatcg gggtggtgca
      901 atcgctcagc tcgtactacg tggcgccggc cttcggccag atgttcttct tcatcctgtt
      961 cctgctggtg atgatcctcc ggcccaacgg gattctcggc cagaagggtg cggcgacgat
     1021 cggaatcaac gactgagacg aacgatgtcc atcgcggctc tcgaccccgc gccgagaaaa
     1081 tccaggcgcc tgcccgcgct gccgcagcgc ggagcggtgt cctggttcga gatcgcgctg
     1141 ctcaccgcct gcctggtgct ggtggcggtt gagctgccgc agaacctgcg cgacgtgatc
     1201 gtgctcacgt tcctgttcgg cgccctcgcg ctcgcctgga acatcgccgg cggctatgcg
     1261 ggattgatct cgttcggcca tgccgccttc ttcggcatcg gcgcctacac ctcgaccatc
     1321 ctcttcgtgc atggcggcct gagcccctgg atcggactgt ggctcggcgc attgctgtcg
     1381 gctgccgccg gcgcgttcct ggccgtcatc tgcgcgcgcc tgcgcggtcc gttcttcatc
     1441 ctgtcgacgc tcgcctgcgc cgaagtcgtg cgcatcggcg cattgaactg ggcctcgctc
     1501 acgggtgggc cggaaggcct ctccatcctt ccggtcgcga gcttggccaa catggtgttc
     1561 gcctccaaga ccgtctatgc ggcgctcatg ctcgcctacc tggtgatctg ctacgcggcc
     1621 accaaggcgc tcgaatgctc ccgctacggc tattacctgt tcgccgtgcg cgacgatgag
     1681 gaagccgcgg gcgcggcggg cgtgaacccg ctgctggcgc gagccggcgc catggccttg
     1741 agcgcggcgt tgacgggcat cggcggctcg ctgttcgccc aatatttcct ctacctcgac
     1801 ccgacgttcg tgatctcacc cgatctttcg tttcaactgg cgctgctgcc ggcggtgggc
     1861 ggcttgggga ccgccatcgg gccggtgctc ggctcgttcc tgatcacgcc gctgtcggaa
     1921 ttgctgcgcg cctatctcgg caacgcggcc gccggccttc atctcgtcat ctacagcgcc
     1981 gggctcatcg tcgcgatgct gtatttcccg tcgggtctcg ccggcgcatt gagccggctc
     2041 gccgggggcg ggaagggcag gccgtgaccg gcatcctcga ggtccggaac gtcagccgcg
     2101 cgttcgacgc attgtgggcc gtgcgcgagg tgagcttcag cgtcgcgccg ggcgagctgc
     2161 tcgggctgat cggacccaac ggggccggca agagcacgct ctacaacctg atcgccggcg
     2221 gcctgaaacc gacgcacggc gagatcgttt ttgccggacg gcgcgtcacc ggctggcgct
     2281 cctaccaggt ggcgcgcatc ggactggcgc gcacgttcca gattcccaaa ccgtatcggc
     2341 agctatccgt caccgagaac gtgatgctca cggcgttcca gcacgagccg tccctggcgc
     2401 gctcgcgggc gctcgcggac gcgacgctcg ccgatgtcgg gctgagcgat tatgcggccg
     2461 ccccgatcgc gacgctcacg gtcggcctgc tcaagcggct ggaagtcgcg cgcgccctgg
     2521 cgatgcgtcc gaagctcgtg ctgttcgacg agatcatggc gggcctcacc cccaccgagg
     2581 tcggattcat gacccggctg gtggccggat tgccggcgcg cggcatcacg gtcctgtggg
     2641 tggagcacgt gctctacgcg atcatgaaga cggcgagccg tatcatggtg ctcaatcgcg
     2701 gcgaggtgat cgccgacggg gcacccgctg ccgttgcccg cgatccggtg gtggtgaagg
     2761 cctatctggg cgaggaaatg agccttgctt gaggtcaggc atctcaacgc gttctacgga
     2821 ccggtgcagg tcctgcacga cgtgagcctg tcggtgccgg taggtcagat cgtcgccctg
     2881 gtcggtgcca atgcggccgg caagagcacg ttgatgttca cgctggccgg cctgcgcacc
     2941 acccataccg gggagctcgt cttcgacggc gagccgctcg gcgcgctctc cgcctatgaa
     3001 cgggtgccgc gcggcctcgt gctggtgccg gagcgccggc gcctgttccc gtttatgacc
     3061 gtgctcgaga acctggagat cggcgcctat tcggctgccg cccgtcctgc tgcgcgcgca
     3121 accttgcagc aggttttcgc gctgctgccg gtgctggacg agcggcgcaa gcaggttgcc
     3181 gggtcgctga gcggcggcga gcagcagatg ctcgccatcg gcagggcgct catggccaag
     3241 ccccgcatgc tgctgctcga cgagccgacc gagggcctgg cgccgatcta tgtgcggcgc
     3301 ttgttcgacc tcatcggcaa tctgcgcagc accgggctca cggtgctcat cgtcgagcag
     3361 aacgtgcacc acgtcctgcg catggccgac cgcggctacg tgctggagaa cggacgcatc
     3421 gtactggagg gacgcggcga ggacctgctc aacgacgagc ggctcaaggt cgcctatctg
     3481 gggctttgac gatgatcacc gacatgcact
//
LOCUS       DASPXW010000357         3891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_732451, whole genome shotgun sequence.
ACCESSION   DASPXW010000357 DASPXW010000000
VERSION     DASPXW010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732451"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2316)
                     /gene="smc"
                     /locus_tag="VEL04_08195"
     CDS             complement(<1..2316)
                     /gene="smc"
                     /locus_tag="VEL04_08195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HYT48387.1"
                     /translation="MRLTQIRLSGFKSFVDPTSIHVPGQLVGVVGPNGCGKSNVIDAV
                     RWVLGETRASALRGDSMQDVIFNGSVNRTPMARASVELMFDNSLGRAAGQWSQYAEIS
                     VKRVLQRDGESSYHINGVHVRRRDITDMFLGTGLGPRAYAIMEQGMISRVIEAKPDEL
                     RVFLEEAAGISKYKERRRETESRLAGTRDNLARISDIRLELGAQVEKLEAQAAVATRY
                     REYHSDLQLKQHLLWFLRRRDAASERERHTHEIENMTHALEAENAQLRNLESRLETAR
                     AAHYQAGDAMNAAQGALYAANAAVASHESQLRYAEETRSRLEGQHTERRAQLAAWREQ
                     RAQLTQALHMWVARAGTAKQKVLSTKDKLETENARLPQAEQAFRAAQERASEARSQLQ
                     QAESALQLEQANRAHVERGAQALGQRRERLESEIHTLTAPDAAAVTALDSKLSGRRAA
                     VAAAQEALEALQNECAMLEERRAAAGEVLGAAQREHAAAEAQLATLRQIQAAAEGNAP
                     LREWLERHGLGQAARLWQKLRIDHGWETAVEAVLRERLHALELTDASRLAAVLADRPP
                     AKASVFDHGARGAAAAAAGLPLATKIHASDPAVSGALGDWLAGVIAVEGTPDRRSREA
                     LVPGAVLVNREGHQFTRHTVSFHASEPQDAGLLARQAEIEALEKRCRELALKVAGAER
                     GQQGIESEAMERTAALEQARLDIAERQKAQHDAQIEHLKATQAEERYRERSAQVRAEL
                     EALDAEAALGARSLAACQAAQARVVEEIADKG"
     gene            2376..2750
                     /gene="queF"
                     /locus_tag="VEL04_08200"
     CDS             2376..2750
                     /gene="queF"
                     /locus_tag="VEL04_08200"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HYT48388.1"
                     /translation="MPSRPSKRLQTFPNPAPERDYRIHMEIPEFTCLCPMTGQPDFAT
                     LLLDYIPDRLCVELKSLKLYVWSFRDAGAFHEAVTNRILDHLVRATRPRHMRLEARFN
                     VRGGIHTTVIAEHRKKGWRGEA"
     gene            2747..3880
                     /gene="dapC"
                     /locus_tag="VEL04_08205"
     CDS             2747..3880
                     /gene="dapC"
                     /locus_tag="VEL04_08205"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HYT48389.1"
                     /translation="MNHLLEKLQPYPFEKLRALVGGHAANPALKPINLSIGEPKHPTP
                     ALVKDALAAGLDGLAVYPATAGTPALRQAIAGWLARRYGIPQPDADTQVLPVNGTREA
                     LFAFAQTVLSPGARVVCPNPFYQIYEGAALLAGAIPLYGDPESWNGVQLVYVCSPANP
                     SGKVLDLQGWQRLFELSDQHRFVIAADECYSEIYFDRPPIGSLQAAHKLNRNGYPRLV
                     AFSSLSKRSNCPGMRSGFAAGSAELLKKFLLYRTYHGSAMNLAVQQASIAAWNDEGHV
                     RENRRLYAEKFEAVLPLLQHPFKAEMPEGGFYLWLRTPIDDQDFVRRLHHEYNVLVLP
                     GSYLARDAGSGNPGSNHVRIALVASLDECVEAIERILRFAHNG"
BASE COUNT          573 a         1358 c         1244 g          716 t
ORIGIN      
        1 tcctttgtcg gcaatttcct cgacgacgcg cgcctgcgca gcctggcaag cggcaagcga
       61 ccgggcgccc agggcggctt cggcgtcgag cgcctcgagc tcggcgcgca cctgggcgct
      121 gcgttcgcgg tagcgctcct cggcctgcgt cgccttgagg tgctcgatct gggcgtcgtg
      181 ctgcgccttc tgtcgctccg caatatcgag ccgcgcctgc tccagcgcgg ctgtgcgctc
      241 catggcctcg ctttcgatac cctgctgccc gcgctcggcg ccggccacct tgagggcgag
      301 ctcgcggcag cgcttttcca gcgcctcgat ttcagcctgc ctcgcgagca gcccggcgtc
      361 ctgcggttcg gacgcatgga agctgacggt atggcgggtg aactgatgtc cttcgcgatt
      421 caccagcacc gctcccggca ccagcgcttc ccgcgatctg cgatcaggcg ttccttccac
      481 ggcgatcact ccggccagcc agtcgcccag cgccccggac accgccggat cggaggcgtg
      541 gatcttagtc gcgagcggca atcccgcggc cgcagccgcc gcgccgcgcg cgccatggtc
      601 gaacacgctc gccttggcgg gcggacggtc ggcgagtacc gccgcgagcc gtgaggcatc
      661 cgtaagctcg agcgcgtgca gccgctcgcg cagcaccgct tcgaccgccg tctcccatcc
      721 gtgatcgatg cgcagcttct gccatagccg cgccgcctgc cccagaccat ggcgttcgag
      781 ccattcgcgc agcggtgcgt tgccttcggc cgcggcctga atctggcgca gggtggcaag
      841 ctgggcctcg gctgccgcat gctcgcgctg cgcggcgccg aggacctcgc cggccgcagc
      901 gcgacgctct tcgagcatgg cgcattcgtt ctgcagcgct tcgagcgctt cctgggcagc
      961 cgcgactgcg gcgcgccgtc ccgagagctt cgagtccaat gccgttacag ccgcagcgtc
     1021 cggtgcggtt agggtatgga tctcgctctc cagccgttcg cggcgctggc cgagcgcctg
     1081 cgcgccgcgc tccacatgcg cgcgattggc ctgctcgagc tgcagcgcgc tttcagcctg
     1141 ctgcagctgg ctgcgcgcct cgctggcgcg ttcctgcgcg gcgcggaacg cctgctcggc
     1201 ctgtggcagg cgcgcatttt cagtttcaag cttatctttt gtactgagaa ccttttgttt
     1261 tgccgtgccg gcccgcgcca cccacatgtg gagcgcctgc gtgagctggg cgcgctgctc
     1321 gcgccacgct gcgagctgcg cgcggcgctc ggtatgctgg ccttcgagcc ggctgcgcgt
     1381 ttcctcggcg tagcgcagct gtgattcatg gctcgcgacc gccgcattcg cggcgtaaag
     1441 cgcgccctgc gctgcattca tcgcgtcgcc cgcctggtag tgcgccgcgc gcgccgtctc
     1501 gaggcgcgat tccagatttc tcagctgggc attctcggcc tccagcgcat gggtcatgtt
     1561 ttcaatttca tgcgtatggc gctcgcgctc gctcgccgcg tcgcgccggc gcaggaacca
     1621 cagcaggtgc tgcttgagct gcaggtcgga atggtattcc cggtagcgcg tggccaccgc
     1681 ggcctgggct tcgagctttt cgacctgtgc cccgagctcg agccggatgt cgctgatgcg
     1741 cgccaggttg tcgcgcgtgc cggcgaggcg gctctccgtt tcgcgccggc gctccttgta
     1801 tttcgagatc cccgcggctt cctcgagaaa cacgcgcagc tcgtcgggct tggcctcgat
     1861 cacccgcgag atcatgccct gctccatgat ggcgtaggcg cgcgggccaa ggccggtgcc
     1921 gaggaacatg tcggtgatgt cgcgccggcg cacgtgcacg ccgttgatgt ggtagctcga
     1981 ttcgccgtcg cgctgcagca cgcgctttac cgagatctcc gcgtactgcg accactgccc
     2041 ggcagcgcgg ccgagcgagt tgtcgaacat cagctcgacc gacgcgcgcg ccatcggcgt
     2101 gcggttgacc gagccgttga agatgacgtc ctgcatcgag tcgccgcgca gcgccgaggc
     2161 gcgcgtttca ccgagcaccc agcgcaccgc atcgatcacg ttcgatttgc cgcagccgtt
     2221 cgggccgacc acgccgacca gttggcccgg cacatggatc gaggttggat cgacgaagga
     2281 cttgaagcct gagagtctta tttgagtaag ccgcacgcaa aaaatgatac catcgcccgc
     2341 tgccttttcc gggtcctgat ttttggaccg agcgcatgcc ctcgcgtccc agcaagcggt
     2401 tgcagacgtt tccgaatccc gcaccggagc gcgactaccg catccacatg gaaattccgg
     2461 agttcacctg cctgtgcccg atgaccgggc aaccggattt cgcgacgctg ctcctcgact
     2521 acatcccgga ccggctgtgc gtcgagctca aatcgctcaa gctctacgtc tggtcttttc
     2581 gcgacgcagg cgcgttccac gaagccgtga ccaaccgcat cctcgaccac ctggtgcgcg
     2641 ccacccggcc gcgccacatg cggctcgagg cgcgcttcaa cgtgcgcggc ggcatccata
     2701 ccacggtgat cgccgagcac cgcaagaagg gctggcgcgg tgaggcgtga atcatctctt
     2761 agaaaagcta caaccctacc cgttcgaaaa actgcgcgcg ctggttggcg ggcatgccgc
     2821 caacccggcg ctcaagccga tcaacttgtc gatcggcgag cccaagcatc cgaccccggc
     2881 cttggtgaag gacgctctgg ccgcggggct cgacgggctg gccgtctatc ccgccacggc
     2941 gggcacgccg gcgctgcggc aggcgatcgc cggctggctg gcgcgccgct acggcatccc
     3001 gcaaccggat gccgacaccc aggtcctgcc ggtcaacggc acgcgcgaag ccttgtttgc
     3061 atttgcacag acggtgcttt cgcccggcgc tcgggtggtc tgcccgaatc cgttctatca
     3121 gatctacgaa ggcgccgcgc tcctcgcagg tgcgataccg ctatatggcg accccgagtc
     3181 ctggaacggc gtgcagctcg tgtacgtctg ctcgccagcc aatccgagcg gcaaggtgct
     3241 cgatctccag ggctggcagc ggctgttcga gctgtcggac cagcaccgct tcgtcatcgc
     3301 cgccgacgag tgctattccg aaatctattt cgaccggccg ccgatcggtt cgctgcaggc
     3361 cgctcacaag ctgaacagga acggttaccc gcgcctggtc gccttctcca gcctgtcgaa
     3421 gcggtccaat tgcccgggca tgcgctcggg ctttgcggcc ggcagcgcgg agctgctgaa
     3481 gaagttcctg ctgtatcgca cctaccacgg ctcggcgatg aacctcgccg tgcagcaggc
     3541 gtcgatcgct gcatggaatg acgaagggca cgtacgcgag aaccggcgcc tgtacgctga
     3601 gaaattcgaa gccgttttgc ctttgctgca gcatcctttc aaggcggaaa tgccggaggg
     3661 cggcttctat ctctggctgc gaacgccgat cgacgaccag gacttcgtgc ggcggctgca
     3721 ccatgaatat aatgtgctgg tgctgcccgg cagctacctg gcgcgcgacg ccggcagcgg
     3781 caatcccgga agtaaccacg ttcgcatcgc actcgtagcg tcgctcgacg aatgcgtcga
     3841 ggccatcgag cgcatcttgc gctttgccca caatggataa gcagagaaag a
//
LOCUS       DASPXW010000358         7573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_106169, whole genome shotgun sequence.
ACCESSION   DASPXW010000358 DASPXW010000000
VERSION     DASPXW010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7573
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106169"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="VEL04_08210"
     CDS             complement(<1..643)
                     /locus_tag="VEL04_08210"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HYT48390.1"
                     /translation="MPATLPPRLGIVILVAVATTFAANHLCARIAFDHGASVATGVAT
                     RATGTALVLLLIMRLQGIRVALPRELRPWALLAGILIAVQSYCLYSAVALIPPALALL
                     VFQTSPMLYVLLSWALGGPAPRPSAFPAMLLALAGLWLALDIRTDHLAARWSEIGAGA
                     SWAFAGGASMACVYYLNANALKSVDGRLRTFVMTAVTAVPVATAGGASDALALP"
     gene            702..1232
                     /locus_tag="VEL04_08215"
     CDS             702..1232
                     /locus_tag="VEL04_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HYT48391.1"
                     /translation="MKFLFDLFPVALFFVAIQIWDIFVATAVAIAASFAQVGWLALRK
                     KRIEPMLWASLAIIVVFGGLTLYLRDKTFILWKPTVLYWLFGSVLAGSAFLGRNLIRA
                     LLSEQMRLPDPVWRRLNWSWVGFFAFMGAANLYVAFNYSEKIWATFKLFGGMGLMLLF
                     VVAQALVLARYAEQKQ"
     gene            1229..1507
                     /locus_tag="VEL04_08220"
     CDS             1229..1507
                     /locus_tag="VEL04_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HYT48392.1"
                     /translation="MNLAEVIRGRLAALEPLSLDLVDESAQHAGHAGWREGGGTHWRL
                     AIVAPCFAGQSAVARHRMVYQALGELMHNPIHALAITARTPDETKGRQ"
     gene            1504..2340
                     /locus_tag="VEL04_08225"
     CDS             1504..2340
                     /locus_tag="VEL04_08225"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HYT48393.1"
                     /translation="MNIRTLAATALAIPALAFAQGAEKDGAKPAAKDSSKAAAKAGAV
                     ATVNGVAVPRARLDALMQQQRARGAPDNEQTRTNVRDELVNREVLMQEAQKAGLGKSP
                     EVLGQLDLARQEITISAYLRDWVRKHPVSDAEVQKEYDRVRAEQGDKEYRARHILVET
                     EDQANSLIAELKKGGKFEELAGKNSKDPGSKERGGDLDWHNPGDFDKQFSEAMMKLEK
                     GKYTETPVRTRYGFHIIQLEDVRAGRFPPFAEVRPRIQQQLVQSRIDELIRGLRAKAK
                     VQ"
     gene            2407..2901
                     /locus_tag="VEL04_08230"
     CDS             2407..2901
                     /locus_tag="VEL04_08230"
                     /inference="COORDINATES: protein motif:HMM:NF013798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HYT48394.1"
                     /translation="MFQVVRGNLLEEPVEAIVNAANGHLAHGGGVAGIIARAAGPALQ
                     EESDLLVQTHGFFPAGSAVVTTAGKLPFKGVIHAVGPRFGEGDEENKLVLALISSFKC
                     ARERGWKSVSFPAVSSGIFAVPLDICARAYLAAVRRAGLSNVRLCLRDQPVIDAVLRE
                     VSKP"
     gene            2898..3488
                     /gene="msrA"
                     /locus_tag="VEL04_08235"
     CDS             2898..3488
                     /gene="msrA"
                     /locus_tag="VEL04_08235"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HYT48395.1"
                     /translation="MKWILALLTALALPAFAQQRPEKATFAAGCFWCTEEAFEKLPGV
                     VSAVSGYMGGNVRNPSYEQVSSGRTGHAEVVQVSFDPAKLSYEKLLDTFWLNHDPTVT
                     NRQFCDSGSQYRPAIFYHSEEQKRLAEASKAKWEKDKPFRQPILTPIVPASDFWPAED
                     YHQDYYKKNSLQYKFYVTGCGRYARLDSLWGELRKK"
     gene            complement(3495..4034)
                     /locus_tag="VEL04_08240"
     CDS             complement(3495..4034)
                     /locus_tag="VEL04_08240"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HYT48396.1"
                     /translation="MCQTAAAQVYRWVDAKGTVHYSDTAPPQGVKATVVDIEAKSGAP
                     SPDSGDCYTLRCQGERLDQRLARREELETRLAAERAASAPRQPRGLEFRRYISIQRGM
                     TEGELLGIAGEPDMLVNQGAAISAPTTVQTGRHLRGAARAVLSLTTYTYLPTPGDPFT
                     TTVTLVGGRVSEIERIRKF"
     gene            complement(4078..4272)
                     /gene="iscX"
                     /locus_tag="VEL04_08245"
     CDS             complement(4078..4272)
                     /gene="iscX"
                     /locus_tag="VEL04_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HYT48397.1"
                     /translation="MKWTDTREIAIELSEKYPDTDPESVNFVDLYKWVLGLPGFDDDP
                     KRCGEKILEAIQMAWIDEAE"
     gene            complement(4269..4619)
                     /gene="fdx"
                     /locus_tag="VEL04_08250"
     CDS             complement(4269..4619)
                     /gene="fdx"
                     /locus_tag="VEL04_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HYT48398.1"
                     /translation="MPKLKVLPHAALCPQGAEIEAQAGKSICDTLLEHHVDIEHACEK
                     SCACTTCHVILRQGFESLEPAEEKEEDLLDKAWGLEASSRLSCQAKVADADLTIEIPK
                     YTINYEREGGGVKK"
     gene            complement(4619..6481)
                     /gene="hscA"
                     /locus_tag="VEL04_08255"
     CDS             complement(4619..6481)
                     /gene="hscA"
                     /locus_tag="VEL04_08255"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HYT48399.1"
                     /translation="MALLQISEPGASPAPHEHRLAAGIDLGTTNSLVATVRSGVATVL
                     PDAHGRPLLPSIVRYLPEGRVEVGYAAQARQADDPRNTIVSVKRYMGRGVKDVAHSES
                     APYDFVDAPGMVQLRTAAGVRSPVEVSADILKVLRDRAERSLGGNLSGAVITVPAYFD
                     DAQRQATKDAGRLAGLNVLRLLNEPTAAAIAYGLENAAEGVYAVYDLGGGTFDLSILK
                     LSRGVFEVIATSGDSMLGGDDFDQRVLCWIVEQAKLAPLAPRDTRLLLVKAREAKESL
                     TYHSESKITAKLSSGELVDLTLDTETFCRITQTLVQKTLGPMRKALRDAGLAVDEVKG
                     VVMVGGATRMPQVQRAVGEFFRRDPLNNLDPDKVVALGAAMQANVLAGNQPTDQDWLL
                     LDVIPLSLGIETMGGLTEKIIPRNTTIPAARAQEFTTFKDGQSGMSFHVVQGERELVS
                     DCRSLARFELKGIPPMVAGAARVRVSFQVDADGLLAVSAKESTSGVEASVSVKPSYGL
                     GDADIERMLRDSFEHANEDVHARALAEHRVEGQRLIEATRSALQADGGLLSADEKTAI
                     EANMQALQKLLPATDHRAIKQAADALNRATEEFAARRMDAGIKRALAGRKIGSF"
     gene            complement(6481..7023)
                     /gene="hscB"
                     /locus_tag="VEL04_08260"
     CDS             complement(6481..7023)
                     /gene="hscB"
                     /locus_tag="VEL04_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HYT48400.1"
                     /translation="MNAVAGKALTSNHFELFGLEPVFALHADSLDSAYRDIQAKVHPD
                     RFAHAGDAERRASLQWTTRVNEAYRTLKDPVQRARHILELHGVDVAFETNTAMPTDFL
                     TQQMELRERLEQAVGRKNADVLDALRRNLAREKQSFESQIGKLIDEKHDYPAAAQLVR
                     KLQFLDKLDAEIDAAYEAVE"
     gene            complement(6989..7309)
                     /gene="iscA"
                     /locus_tag="VEL04_08265"
     CDS             complement(6989..7309)
                     /gene="iscA"
                     /locus_tag="VEL04_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HYT48401.1"
                     /translation="MITLTEKAADHVRTFLARRGRGVGLRVGVRTSGCSGLAYKLEFA
                     DAVNPEDHQFESHGVNLYVDPKSLQYIDGTELDYAREGLNEGFKFRNPRVKDECGCGE
                     SFNV"
     gene            complement(7306..>7573)
                     /locus_tag="VEL04_08270"
     CDS             complement(7306..>7573)
                     /locus_tag="VEL04_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HYT48402.1"
                     /translation="GVIEDAKFKTYGCGSAIASSSLVTEWVKGKTVNEAATIKNTQIA
                     EELALPPVKIHCSILAEDAIKAAVEDYRKKHGLEAGEEKAKKVA"
BASE COUNT         1271 a         2498 c         2505 g         1299 t
ORIGIN      
        1 tcggcagcgc cagggcatcc gatgcgccgc ccgcggtggc gaccggcacg gcggtgaccg
       61 cagtcatcac gaaggtgcgc agcctgccgt cgacggactt gagcgcgttt gcgttcaggt
      121 aataaacgca cgccatgctg gccccgcccg cgaatgccca actcgccccg gcgccgatct
      181 cgctccagcg cgccgccaga tggtcggtgc gtatatcgag cgcgagccac agcccggcga
      241 gcgcaagcag catcgcgggg aaggcgctcg gccgcggcgc cgggcctccc agggcccagg
      301 aaagcagcac gtaaagcatg ggagaggtct gaaacaccag cagcgccagc gccggcggta
      361 taagcgcgac ggccgagtac aggcaatagc tctgtaccgc gatcagaatt cccgccagca
      421 aggcccaggg gcgcagctcg cgcggcaaag caacgcgaat cccctgcagg cgcatgatca
      481 gaagcagcac cagcgcggtg ccggtcgcgc gcgtggcgac gccggtcgcc acgcttgcgc
      541 catgatcgaa agcgatgcgc gcgcacagat ggttcgccgc gaacgtggtt gccacggcga
      601 ccaggatgac gatgccaagt cgaggtggta acgtcgccgg catgggggcc gacgttatca
      661 cgcccgacgg caaacgcacg ctggtagaat cgcgccactg aatgaagttt ctgttcgatc
      721 ttttccccgt cgcccttttc ttcgtcgcga tccagatctg ggacatcttc gtcgccaccg
      781 cggtggcgat cgcggcgagc ttcgcgcagg tcggctggct cgcgctgcgc aagaagcgga
      841 tcgagccgat gctctgggcg agcctggcga tcatcgtcgt cttcggcggc ctcacgctgt
      901 acctgcgcga caagaccttc atcctgtgga agccgacggt gctctactgg ctgttcggct
      961 cggtgctggc gggctcggcg ttcctcggcc gcaacctgat ccgcgcgctg ctgtcggagc
     1021 agatgcggct gcccgacccg gtgtggcggc ggctcaactg gagctgggtc ggcttcttcg
     1081 ccttcatggg cgccgccaac ctctacgtgg cgttcaatta ttccgagaag atctgggcca
     1141 cgttcaagct gttcggcggc atggggctga tgctgctgtt cgtggtggcg caggcgctgg
     1201 tcctcgcccg ctacgccgag cagaagcaat gaatctggcc gaagtcatcc gtgggcgcct
     1261 cgcggcgctc gagccgctgt cgctcgacct ggtggacgag agcgcgcagc acgccggcca
     1321 tgccggctgg cgcgagggcg gcggcacgca ctggcggctc gcgatcgtag cgccgtgctt
     1381 tgccgggcag agcgccgtgg cgcgccaccg catggtttat caggcactcg gcgaactgat
     1441 gcacaatccc atccacgcgc tcgccatcac ggcgcgcacc cccgacgaaa cgaaaggaag
     1501 gcaatgaata tccgaaccct cgccgccacc gcgctggcga tcccggccct ggcttttgcc
     1561 caaggcgcag agaaggacgg cgccaagccg gcggcgaagg acagcagcaa ggccgccgcc
     1621 aaggcgggtg ccgtcgccac cgtgaatggc gtggcggtgc cgcgcgcgcg cctggacgcc
     1681 ctgatgcagc agcaaagggc gcgcggcgcc ccggacaacg agcagacccg caccaacgtg
     1741 cgcgacgagc tcgtcaaccg cgaggtcctg atgcaggagg cgcagaaggc cggcctgggc
     1801 aaaagcccgg aagtgctggg ccagctcgat ctcgcgcgcc aggaaatcac catcagcgcc
     1861 tacctgcgcg actgggtacg caagcacccg gtgagcgacg ccgaggtgca gaaggagtac
     1921 gaccgcgtca gggccgagca gggcgacaag gaataccggg cgcgccacat cctggtcgag
     1981 accgaggatc aggcgaactc cctgatcgcg gagctcaaga agggcggcaa gttcgaggag
     2041 ctggcgggca agaactccaa ggacccgggc agcaaggaac gcggcggcga cctcgactgg
     2101 cataaccccg gcgacttcga caagcagttc tccgaggcga tgatgaagct cgagaaaggc
     2161 aagtacacgg aaacgccggt gcgcacgcgc tacggcttcc acatcatcca gctcgaggac
     2221 gtgcgcgccg ggcgtttccc gccgttcgcc gaagtaaggc cgcgcatcca gcagcagctg
     2281 gtgcagagca ggatcgacga gcttatccgg gggctcaggg cgaaagcgaa agtacagtaa
     2341 aagcggcgcg aagccgccac gcaaacgcaa gcaatcctcg cgcaaaaaga aatagccccc
     2401 ggccggatgt tccaggtggt gcggggcaac ctgctcgaag agccggtcga ggccatcgtc
     2461 aacgccgcga acggtcacct cgctcacggc ggcggggtgg cgggaatcat cgcgcgcgcc
     2521 gccgggccgg cgctgcagga ggaatcagac cttctggttc aaacacatgg attcttccct
     2581 gcaggaagcg cagtggtcac gaccgccgga aaattgccgt tcaagggcgt gatccacgcc
     2641 gtcgggccgc gctttggaga gggcgacgag gaaaacaagc tcgtcctagc attgatctcc
     2701 tctttcaaat gcgcgaggga acgtggctgg aagtcggtgt cgttcccggc tgtctcgagc
     2761 ggtatcttcg ccgttccgct cgatatctgc gcgcgcgcct acctggcggc cgtgcgccgc
     2821 gccgggctca gtaatgtcag gctatgcctg cgcgaccaac cggtcatcga cgcggtgctg
     2881 agggaggttt cgaaaccatg aaatggattc ttgcgttgct gacggcgctg gcattgccgg
     2941 cgttcgcgca gcaaagaccc gaaaaagcga ctttcgccgc gggctgcttc tggtgcaccg
     3001 aggaggcctt cgagaagctc ccgggcgtgg tgtcggcggt gtcgggctac atgggaggca
     3061 acgtcaggaa cccgagctac gagcaggtgt catcgggccg caccggccac gccgaggtag
     3121 tgcaggtgag cttcgatccg gcgaagctgt cctatgaaaa gctgctcgac accttctggc
     3181 tgaaccacga tccgacggtg accaaccgcc agttctgcga ctcgggctcg cagtaccggc
     3241 cggcgatctt ctaccacagc gaggagcaga agcgcctcgc cgaggcctcc aaggcgaaat
     3301 gggagaagga caagccgttc cgccagccga tcctcacgcc gatcgtgccg gcgagcgact
     3361 tctggcccgc cgaggactac caccaggact actacaagaa gaactcgctg cagtacaagt
     3421 tctacgtcac gggctgcggc cgctacgccc ggctcgacag cctgtggggc gagctgcgca
     3481 agaaataggc tcggctagaa cttcctgatg cgctcgattt cgctgacgcg gccgccgacc
     3541 agcgtaacgg tggtggtgaa gggatcgccc ggcgtcggca ggtaggtgta cgtcgtgagc
     3601 gacaagaccg cgcgcgcggc gccgcgcaga tggcgcccgg tctgcaccgt ggtcggtgcc
     3661 gagatcgccg cgccctggtt gaccagcatg tcgggctcgc ccgcgatgcc gagcagctcg
     3721 ccctcggtca tgccgcgctg gatcgagatg tagcggcgga actccaggcc gcgcggctgc
     3781 cgcggcgcgc tggcggcgcg ctcggccgcc aggcgggtct ccagctcctc gcggcgggcg
     3841 aggcgctggt cgagccgctc gccctggcag cgcagcgtgt agcagtcgcc gctgtcgggc
     3901 gatggcgccc cgctcttcgc ttcgatgtcg acgacggtgg ccttgacgcc ttgcggcggt
     3961 gcggtgtccg aatagtgcac cgtgccctta gcgtccaccc agcggtacac ctgcgccgct
     4021 gcggtttgac acatgaaaaa tatcgccagg aggaaggcaa gtcgcacaaa aaccatccta
     4081 ctccgcctcg tcgatccagg ccatctggat ggcctcgaga attttctcgc cgcagcgctt
     4141 ggggtcgtcg tcgaacccgg gcagccccag cacccatttg tagaggtcga cgaagttgac
     4201 ggactccgga tcggtgtccg ggtatttttc gctcagctcg atcgcgatct cccgggtgtc
     4261 ggtccacttc atttcttcac ccctcctccc tcgcgctcgt agttgatggt gtacttggga
     4321 atctcgatcg tcaggtctgc gtcggcaact ttcgcctggc acgacagccg cgagctggcc
     4381 tccaggcccc aggccttgtc gagcaggtct tcctccttct cctccgccgg ctcgagcgac
     4441 tcgaagccct ggcgcaggat cacgtggcag gtggtgcagg cgcaggattt ttcgcaggcg
     4501 tgctcgatgt cgacgtggtg ctcgagcagc gtatcgcaga tcgacttgcc cgcctgcgcc
     4561 tcgatctcgg cgccctgcgg gcatagcgcg gcgtgaggca gcaccttgag cttcggcatc
     4621 agaagctgcc gattttcctg cctgcgagag cgcgcttgat gcccgcgtcc atgcggcgcg
     4681 cggcgaactc ctcggtggcg cggttgagcg cgtcggccgc ctgcttgatc gcccggtgat
     4741 cggtcgcggg cagcagtttc tgcaaagcct gcatgtttgc ttcgatcgcc gttttctcgt
     4801 ctgcggaaag caaaccgccg tcagcctgca acgcggagcg ggttgcctcg atgagccgct
     4861 ggccctcgac gcggtgctcg gcgagcgcgc gcgcgtgcac gtcttcgttg gcgtgctcga
     4921 acgagtcgcg cagcatgcgc tcgatatccg cgtcgccgag gccataggag ggcttcacgc
     4981 tgaccgaggc ctcgacccct gaagtgcttt cttttgcaga gacagcgagc agcccatccg
     5041 cgtcgacctg gaagctcacg cgcacgcgcg ccgcgccggc gaccatcggc gggatgccct
     5101 tcagctcgaa gcgcgcgagc gagcggcagt ccgacaccag ctcgcgctcg ccctgcacca
     5161 cgtggaagct catgccgctc tgcccgtcct tgaaggtggt gaattcctgc gcgcgcgccg
     5221 ccgggatggt ggtattgcgc gggatgatct tctcggtgag cccgcccatg gtctcgatgc
     5281 cgagcgaaag cgggatcacg tcgagcagca gccagtcctg gtcggtcggc tggttgccgg
     5341 cgagcacgtt ggcctgcatc gcggcgccga gcgcgaccac cttgtcggga tcgaggttgt
     5401 tgagcggatc gcgcctgaag aactcgccga ccgcgcgctg cacctgcggc atgcgggtcg
     5461 cgccgcccac catcaccacg cccttgacct cgtccaccgc gaggcccgca tcgcgcagcg
     5521 ccttgcgcat cggcccgagg gtcttctgca ccagcgtttg cgtgatgcgg cagaaggtct
     5581 cggtgtcgag cgtcaggtca accagctcgc cggaggagag tttcgccgtg atttttgatt
     5641 ctgaatgata ggtcaaggac tccttcgcct cgcgcgcctt gaccagcagc aagcgcgtgt
     5701 cgcgcggcgc gagcggcgcg agcttcgcct gctccacgat ccagcacagc acgcgctggt
     5761 cgaaatcgtc gccgccgagc atcgagtcgc cgctggtcgc gatcacctcg aacacgccgc
     5821 gcgacagctt cagtatcgag aggtcgaagg tgccgccgcc gaggtcgtag actgcgtaca
     5881 ctccttcggc ggcattttcc aggccgtagg caatcgcagc tgccgtaggt tcattcaata
     5941 atctgaggac attaaggccc gcgagcctcc cggcgtcctt ggtggcctgg cgctgggcgt
     6001 cgtcgaagta ggcggggacg gtgatcaccg ctcccgacag gtttcctccc aggcttcttt
     6061 ccgctctgtc cctgaggacc ttgaggatgt cggcagacac ctccaccgga ctcctcacgc
     6121 ccgccgcggt gcgcagctgc accatgccgg gagcgtcgac gaagtcgtac ggcgcgctct
     6181 cgctatgcgc gacgtccttc acaccccggc ccatgtagcg cttcaccgag acgatggtgt
     6241 tcctcggatc gtcggcctgg cgtgcctgcg ccgcgtagcc cacctcgacc cgcccttcgg
     6301 gcaggtagcg cacgatcgag ggcagcagcg gccggccgtg ggcatcgggc agcaccgttg
     6361 ccacgccgct ccttaccgtc gccaccagcg agttggtcgt gccgaggtcg atgcctgcag
     6421 cgagccggtg ctcgtgcggc gccggggaag cgccgggctc ggagatctgc agcagagcca
     6481 ttattcgacc gcctcgtagg ccgcgtcgat ctcggcgtcc agcttgtcga ggaactgcag
     6541 cttcctcacc agctgcgccg cagcgggata gtcatgcttt tcatcgatga gcttgccgat
     6601 ctggctttcg aaggattgtt tctctcttgc gagattcctg cgaagagcat ccagcacatc
     6661 ggcattcttc cggccgaccg cttgttccag ccgctcgcgg agctccatct gctgcgtcag
     6721 gaagtccgtc ggcatggcgg tgttggtctc gaacgcgacg tcgacgccgt gcagctcgag
     6781 gatgtgcctc gcgcgctgca ccgggtcctt cagcgtgcga taggcctcgt tgacgcgcgt
     6841 ggtccactgc agcgaggcgc ggcgctcggc gtcgccggca tgcgcgaagc ggtcggggtg
     6901 aaccttcgcc tggatatcgc ggtacgcaga gtcgagcgag tcggcgtgca gcgcaaaaac
     6961 cggctcaagc ccgaacaact cgaaatgatt agacgttaaa gctttccccg caaccgcatt
     7021 catccttgac cctggggttg cggaacttga agccttcgtt cagcccctcg cgcgcgtagt
     7081 cgagctcggt gccgtcgatg tactgcaggc ttttcggatc gacgtagagg tttacgccgt
     7141 ggctctcgaa ctggtggtcc tcggggttca ccgcatcggc gaactcgagc ttgtacgcca
     7201 ggcccgagca gcccgaagtg cgcacgccga cgcgcagacc cacgcccctg ccgcgcctgg
     7261 cgaggaaggt gcggacgtga tcagccgctt tctcggtcaa ggtaatcaag ctactttctt
     7321 cgccttctct tccccggctt ccaggccgtg cttcttgcga tagtcctcca ctgcggcctt
     7381 gattgcgtcc tcggcgagga tcgagcagtg gatcttcacc ggcgggagcg ccagctcctc
     7441 ggcgatctgc gtgttcttga tggtggcggc ctcgttcacg gtcttgccct tgacccactc
     7501 ggtgacgagc gaggacgagg cgatcgcgct gccgcagccg taggtcttga acttcgcgtc
     7561 ctcgatgacg ccg
//
LOCUS       DASPXW010000359         2737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1241549, whole genome shotgun sequence.
ACCESSION   DASPXW010000359 DASPXW010000000
VERSION     DASPXW010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241549"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VEL04_08275"
     CDS             <1..444
                     /locus_tag="VEL04_08275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HYT48403.1"
                     /translation="WYGPMGVEAKAFHGFDGHGLKMLSQSGVPTGLLTGRSSGAVGSR
                     ARELGIAHLLQGVGDKRAAFEELLERLGLERETVGYMGDDLVDLPVLTRCGLACAPRE
                     APEEVRNRVHYIASAPAGFGAAREVCELLMRAQGTLERALQEYLR"
     gene            441..1013
                     /gene="lptC"
                     /locus_tag="VEL04_08280"
     CDS             441..1013
                     /gene="lptC"
                     /locus_tag="VEL04_08280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HYT48404.1"
                     /translation="MRLSTARLFPLALMFVLALLTFWLERTVREEEPHPSLRRHDPDY
                     LATNFSGTTYNRAGGAETVVLATRMLHYPDDDSTELVSPRVVQAKPAEPRFTVSADRG
                     ALSREGDEIFLYDNVLLLREADAERPEARMTTSFLHIVRDRSLVRTDREVRIVEGRRS
                     LSGRGMEYNNESRELMLRSDVQARFEAAAP"
     gene            1010..1549
                     /gene="lptA"
                     /locus_tag="VEL04_08285"
     CDS             1010..1549
                     /gene="lptA"
                     /locus_tag="VEL04_08285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HYT48405.1"
                     /translation="MRRAPLLIPIVALLAWPVAVFADKADRDKPTQIEANRMSADDTR
                     RLTIFEGAVVLTRGTITLRAERLVVRQDTEGYQFATATGKPARFRQRQEPKPPDTEGG
                     WLEGEALRMEMDDRSGKIELFDNARVNRGGDEVAGNYIFVDQRSDFFSVSAGRGPGAA
                     DGRVRAILQPKAPTAEAGK"
     gene            1557..2279
                     /gene="lptB"
                     /locus_tag="VEL04_08290"
     CDS             1557..2279
                     /gene="lptB"
                     /locus_tag="VEL04_08290"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HYT48406.1"
                     /translation="MSQLASQKLRKRYKSRTVVQDVSLEVSSGEVVGLLGPNGAGKTT
                     CFYMIVGLIPADAGSIHLDHTELTHLPIHRRARLGISYLPQENSVFRRLTVEENVQAI
                     LELQGLAAGEIEARLAELLKDLNIAHLRKHAAISLSGGERRRLEIARALGTRPTFILL
                     DEPFAGVDPIAVLDIQRIIRFLKERGIGVLITDHNVRETLGICDRAYIINEGAVLASG
                     HPEEIVYNDSVRRVYLGENFRM"
     gene            2316..>2737
                     /locus_tag="VEL04_08295"
     CDS             2316..>2737
                     /locus_tag="VEL04_08295"
                     /inference="COORDINATES: protein motif:HMM:NF012530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HYT48407.1"
                     /translation="MKPSLQLRLSQHLTLTPQLQQSIRLLQLSTVELNQEIERILMEN
                     PALEREDHDGEADAPHAPAAAGPAAQEKASEPAEADWPVDIAAGWRGGDDEDSDRSFS
                     APDTPTLRDHLCTQLTLTNLGERDRVFVALLIDALDDDG"
BASE COUNT          459 a          945 c          937 g          396 t
ORIGIN      
        1 tggtacggcc cgatgggcgt tgaagcgaag gcgttccatg gtttcgacgg ccatgggctg
       61 aagatgctct cgcaaagcgg cgtgccgacc gggttgctga cggggcgcag ttccggtgcg
      121 gtcggatccc gcgccaggga gctcggcatc gcgcacctcc tgcagggcgt cggcgacaag
      181 cgcgccgcct tcgaggagct gctggagcgc cttggcctgg aacgcgagac agtgggctac
      241 atgggagacg acctggtcga tctgcccgtg cttacgcgct gcgggctcgc ctgcgcgccg
      301 cgcgaagccc ccgaggaagt gcgaaaccgt gtgcactaca tcgcttccgc gcccgccggg
      361 ttcggcgccg cgcgcgaagt gtgcgagctg ctgatgcgcg cgcagggcac gctcgagcgc
      421 gcgctgcagg aatacctgag gtgaggctgt cgaccgcgcg gctgttcccg ctcgcgctga
      481 tgttcgtgct ggcgctgctg acgttctggc tcgagcgcac ggtgcgcgaa gaggagccgc
      541 acccgtcgct gcgccggcat gacccggact acctggcgac caatttcagc ggcaccacgt
      601 acaaccgcgc aggcggcgcc gagacggtcg tgttggcgac gcgcatgctg cactatcccg
      661 acgacgattc gaccgagctg gtctcgcccc gggtggtgca ggccaagccc gccgagccac
      721 gcttcaccgt gagcgccgat cgcggcgcgc tctcgcgcga gggcgatgag atcttccttt
      781 acgacaacgt gctgctgctg cgcgaggcgg acgccgaacg tcccgaagcg cgcatgacga
      841 cgagcttcct gcacatcgtc agggaccgtt cgctggtgcg caccgaccgc gaagtgcgca
      901 tcgtcgaggg ccgccgctcg ctgtcggggc ggggaatgga atacaacaac gagtcgcgcg
      961 agctcatgct gcgcagcgac gtgcaggcgc gcttcgaggc ggccgcgccg tgagacgcgc
     1021 gccgcttctc atccccatcg tcgcgctgct tgcctggccg gtggccgtgt tcgccgacaa
     1081 ggccgaccgc gacaagccga cgcagatcga agccaaccgc atgagcgccg acgacacccg
     1141 gcgcctgacc atcttcgagg gcgcggtggt gctcacgcgc ggcacgatca cgctgcgcgc
     1201 cgagcgcctc gtcgtgcgcc aggacaccga gggctaccag ttcgccaccg ccaccgggaa
     1261 accggcgcgc tttcgccagc gccaggagcc gaagccgccc gacaccgaag gcgggtggct
     1321 cgaaggcgag gcgctgcgca tggaaatgga cgaccgcagc ggcaagatcg agctgttcga
     1381 caatgcgcgc gtcaaccgcg gcggcgacga ggtggcgggc aactacatct tcgtcgacca
     1441 gcgctcggac ttcttctcgg tgagcgcggg cagaggcccg ggcgccgcgg acggccgggt
     1501 gcgggccatc ctgcagccta aggcaccgac cgcggaagcc ggaaaatgag cgcgcggtga
     1561 gccagctcgc ctcgcagaag ctgcgcaagc gctacaagtc gcgcaccgtg gtgcaggacg
     1621 tctcgctgga ggtctcgagc ggcgaggtgg taggcctgct cggcccgaac ggcgcgggca
     1681 agacgacctg cttctacatg atcgtcggcc tgatccccgc cgatgccggc agcattcatc
     1741 tcgaccatac cgagctcacg cacttgccga tccaccggcg cgcgcgcctg gggatttcgt
     1801 acctgccgca ggaaaactcg gtgttcaggc gcctcacggt ggaagagaac gtgcaggcga
     1861 tcctcgagct gcagggcctc gccgcgggcg agatcgaggc gcggctcgcc gagctgctga
     1921 aagacctgaa catcgcccac ctgcgcaagc acgccgcgat ctcgctctcg ggcggcgagc
     1981 gccgccgcct ggaaatcgcg cgcgcgctcg gcacccgccc gaccttcatc ctgctcgacg
     2041 agccgttcgc cggcgtcgac ccgatcgccg tgctcgacat ccagcgcatc atcaggttcc
     2101 tgaaggaacg cggcatcggg gtgctcataa cggatcacaa cgtgcgggag acgctcggca
     2161 tctgcgaccg cgcctacatc atcaacgagg gcgcggtgct cgcctccggc caccccgagg
     2221 agattgtcta taatgacagc gtgcgccggg tttatctggg tgagaacttt cgcatgtagt
     2281 ttttagcgcg gttccggcac actgcagaac atctgatgaa accctcgctg cagttgcgcc
     2341 tatcgcagca cctcacgctg acgcctcagc tgcagcagtc gatccgcctg ctgcagctct
     2401 ccacggtcga gctcaaccag gagatcgagc gcatcctgat ggaaaacccg gcgctcgagc
     2461 gcgaggacca cgacggcgag gccgacgcgc cgcacgcacc cgctgcagcg ggcccggcgg
     2521 cgcaggaaaa agcctcggag ccggccgagg ccgattggcc ggtcgacatc gcggcgggct
     2581 ggcgcggcgg cgacgacgaa gacagcgacc gcagcttctc cgcgcccgac accccgacgc
     2641 tgcgcgatca cctgtgcacg cagctcacgc tcaccaacct cggcgagcgc gaccgcgtgt
     2701 ttgtggcgct gctgatcgac gcgctcgacg acgacgg
//
LOCUS       DASPXW010000360         4860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_355390, whole genome shotgun sequence.
ACCESSION   DASPXW010000360 DASPXW010000000
VERSION     DASPXW010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355390"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="VEL04_08300"
     CDS             <1..844
                     /locus_tag="VEL04_08300"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DotU family type IV/VI secretion system protein"
                     /protein_id="HYT48408.1"
                     /translation="APAAHLELLEFFYVCLALGFEGRYRGGEGARQALAQARARLCEQ
                     IGALRPQGSAELSSRWRGAEIRPRRVSGALALWGVASGCVVLLAGLYFAYSVSLGALS
                     DPVARELARLKPPAVAFQNSSVAPQESAVAHQRRAGASLGEQLSEAIGRREVEVLDVG
                     GAAVIVLKADQLFASGSARLDAGLRPLVVRIAETLDRLPGAIVITGHSDDVPIRTARF
                     PSNWELSAERARSVSALMATRLREPGRLRAEGVADSEPLVANDGPANRARNRRVAIIL
                     RPAP"
     gene            841..2772
                     /gene="tssM"
                     /locus_tag="VEL04_08305"
     CDS             841..2772
                     /gene="tssM"
                     /locus_tag="VEL04_08305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HYT48409.1"
                     /translation="MSAGWLGGRWAVGAAAWAAFSAAIWLAGDTLVVGGMRPLDGEVE
                     RLTLIAALAAAWLGWELWRARKSLRENERLLEGLAGGTAGEDSAERAEHELSVLRERF
                     EDALAVLRKARFRGAGGERRTVAELPWYMFIGAPGSGKTTALLNAGLRFPLGDPRAER
                     ALQGVGGTRNCDWWFTDEAVLLDTAGRYTTQESDHEADSAAWLGFLELLKQFRPRQPL
                     NGVIVTLSVADLAHWTEDEIARYAAHVRERVRELYTRLGVRLPVYVMVTKADLLAGFN
                     EFFAEFDAERRAQVWGATFDASELPSQARPVAARFNEEFAHLERRLYALLPGRLQEER
                     DLQRRAAIYRFPQQFRSGGPPAASFLDAAFGGGWAGEPPLLRGVYFASGTQEGSPIDR
                     VLGTLARSFNLERKVQPPNIGSGKSFFLRRLLREVIFGEAGLAGIAPGEERRRRLAGT
                     AAYAAIGALGVALALAWTASFLGNRGLVAAAGENAAAAKRELDSLSAIRAGDEARLVT
                     LLNRLRELRQVDSSWSLHWGLYQGEKLAAQAERAYRNMLHETLLPHLADALETKLRAA
                     PSREAFEGYVALYDAVRDSGPLERAAAALWQLPKKAQGELEAHLRAALADALPALPRG
                     RDDALIDSARRKLASGAKT"
     gene            2769..3539
                     /gene="tagF"
                     /locus_tag="VEL04_08310"
     CDS             2769..3539
                     /gene="tagF"
                     /locus_tag="VEL04_08310"
                     /inference="COORDINATES: protein motif:HMM:TIGR03373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagF"
                     /protein_id="HYT48410.1"
                     /translation="MIGARAAPGAAAPGSFPTGWYGKIPATGDFIARRIPASFSESWD
                     RWLQAAVEGSRVQLGARWRDSFVSMPAWRFILAPGVLTQNAWAGLMAPSVDAVGRYFP
                     LTVAAALPAASVDQVATLLAAAAWFDQIEAIALKAIAPAADTAAIDAALAERPFKAAW
                     LQLPRAGDHAMPIKGAKSQMLWVPLPGRAPHERPPASLYELAERLVEPYSAWLAEESE
                     VFGRCLLLCEALPAAGQYCAMMDGRWIEHGWGRRKIGA"
     gene            3549..4697
                     /gene="tagH"
                     /locus_tag="VEL04_08315"
     CDS             3549..4697
                     /gene="tagH"
                     /locus_tag="VEL04_08315"
                     /inference="COORDINATES: protein motif:HMM:TIGR03354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HYT48411.1"
                     /translation="MPITVKIFNHSAARPTLYASHDFDRPPVSIGRDAACTMVLDDPH
                     KHISRFHVEIEEEGGNYWMSVVSKVNPVMVKGRRYGPGTRLTLKSGDTFEIGEYEIHV
                     MLSEGRTMPGAAPAGGGSIFEESTFVAGRAPAAAASVFEESTFVAGRAPAAAASVFEE
                     STLVGAAKAPAPAPRVAPAAPAGVVADLVGVFLEGTGLPHKDLSTAEGERLLRECGTI
                     LRAAIEGIMMLLIARAEMRKEFQAEDRTMVETRDNNPLKLMSDPHEAMAFLFDPAERI
                     EGFLDPVQAVSDACEDLRAHELALMAGMRAAILGALRRLDPHVLERAFEKSSGGFSLA
                     SRKARLWELFVAHQEKLSHDAQEDFNKVFGREFMGAYQSQLRRLRGGR"
BASE COUNT          673 a         1692 c         1761 g          734 t
ORIGIN      
        1 cgcgccggcc gcccacctcg agctgctcga attcttctat gtctgcctcg cactcggctt
       61 cgagggccgc taccgcggcg gcgagggcgc gcgccaagcg ctcgctcagg ctcgcgcgcg
      121 cctttgtgag cagattggcg cactccggcc gcagggcagc gcggagcttt ccagccgctg
      181 gcgcggcgcc gaaatccgcc cgcgccgcgt ctccggtgcg ctcgcgctct ggggcgtagc
      241 atcgggctgc gtggtcctgc tggccgggct gtacttcgcc tacagcgtga gcctcggcgc
      301 gctctccgat cccgtggcgc gcgagctcgc gcgattgaag ccgcctgccg ttgcgtttca
      361 gaactcgtcg gttgcgcctc aggagtcggc cgttgcgcac caaaggcgtg ccggggcttc
      421 cctcggcgag cagctgagcg aggccatcgg gcggcgcgaa gtcgaggttc tcgacgtcgg
      481 cggcgcagcc gtcatcgtgc tcaaggccga tcagctgttc gcctcgggca gcgcgcgtct
      541 cgatgccggc ttgcgtcccc ttgtggtgcg catcgccgag acgctcgacc gccttcccgg
      601 tgcgatcgtg attacggggc attcggatga tgtgccgatc cgcaccgcgc gctttccgtc
      661 caattgggag ctttccgccg agcgcgcccg ctccgtctcg gcgctgatgg ccacgcgcct
      721 gcgcgagccg ggcaggctgc gcgccgaagg cgtcgccgat tcggagccgc tggtggccaa
      781 cgacgggcct gccaatcgcg ccaggaaccg gcgtgtcgcc atcatcctga ggcccgcgcc
      841 gtgagcgccg gatggctggg aggccgctgg gcggtcggcg cggctgcgtg ggcggcgttc
      901 tcggctgcaa tctggctggc cggcgacacg ctggtggtcg gcggcatgcg gccgctggac
      961 ggcgaggtgg agcgccttac gctgatcgct gcgctagccg ccgcctggct cggctgggag
     1021 ctgtggcgcg cgcgcaaatc gctgcgggaa aacgagcggc tgctcgaggg cctcgccggc
     1081 ggcacggcag gcgaagactc ggccgagcgt gccgagcacg agctctcggt gctgcgcgaa
     1141 cgcttcgagg acgcgctcgc cgtgctgcgc aaggcgcgct tccgcggcgc cggcggcgag
     1201 cgccgcacgg tcgctgagct gccgtggtac atgttcatcg gcgctcccgg ctcgggcaag
     1261 accaccgcgc tgctcaacgc ggggctgcgc ttcccgctcg gcgacccgcg tgccgagcgc
     1321 gcgctgcagg gcgtcggcgg cacgcgcaac tgcgactggt ggttcaccga cgaagccgtg
     1381 ctgctcgaca ccgccggtcg ctacaccacg caggaaagcg atcacgaagc cgacagcgct
     1441 gcctggctcg gttttctcga gctgctcaag cagtttcgcc cgcgccagcc gctcaacggc
     1501 gtgatcgtca cgctgagcgt ggcggacctg gcgcactgga ccgaggacga gatcgcgcgc
     1561 tacgcggcac acgtgcgcga gcgcgtgcgg gagctctaca cgcggctcgg cgtgcggctg
     1621 ccggtgtacg tgatggtcac caaggcggac ctgctcgccg gcttcaacga gttcttcgcc
     1681 gagttcgacg ccgagcgccg cgcgcaggtc tggggcgcga ctttcgatgc gagcgaactg
     1741 ccctcgcagg cgcgtccggt ggcggcgcgc ttcaacgagg agttcgcgca cctcgagcgc
     1801 cggctctacg cgctgctgcc ggggcggctg caggaagagc gcgatctgca gcgccgcgcc
     1861 gcgatctacc gctttccgca gcagttccgc tcgggcgggc cgcccgccgc gagcttcctc
     1921 gacgcagctt tcggcggcgg ctgggccggc gagccgccgc tgctgcgcgg cgtgtacttc
     1981 gcgagcggca cgcaggaagg cagccccatc gatcgcgtgc tcggcaccct ggcgcgcagc
     2041 ttcaacctgg agcgcaaggt gcagccgccg aacatcggca gcggcaagag cttcttcctg
     2101 cgccggctgc tgcgcgaggt gatcttcggt gaagccgggc tcgccggcat cgcccccggc
     2161 gaggagcgca ggcgcaggct ggcaggcaca gcggcctacg ccgccatcgg cgcgctcggc
     2221 gtcgcgctgg cgctcgcctg gaccgcgagc ttcctcggta accgcggtct ggtcgcggcg
     2281 gcgggcgaaa acgccgctgc ggcgaagcgc gagcttgata gcctgtctgc gattcgcgcc
     2341 ggcgacgagg cgcgcctggt gacgctgctc aaccgcctgc gcgagctgcg ccaggtcgac
     2401 tcgtcctggt cgctgcactg ggggctgtac cagggagaaa agctcgccgc ccaggcggag
     2461 cgcgcttacc gcaacatgct gcacgagacg ctgttgccgc acctcgcgga cgcgctcgag
     2521 acgaagctgc gcgccgcgcc gagccgcgag gccttcgaag gctacgtggc gctctacgac
     2581 gccgtgcgtg attccgggcc gctagagcgg gcggcagccg cgctgtggca actgccgaaa
     2641 aaggcgcagg gcgagctcga agcgcacctg cgcgcggcgc tcgccgatgc tctgcccgcg
     2701 ctgccgcgcg gccgcgacga tgcgctgatc gacagcgcgc gccgcaagct cgcgagcggg
     2761 gcgaagacat gatcggcgcg cgagccgcgc ccggcgcggc ggcgccgggg agcttcccga
     2821 cggggtggta cggcaagatc cccgctaccg gcgacttcat agcgcggcgc atcccggcgt
     2881 cgttcagcga atcctgggac cgctggctcc aggcagcggt cgaaggcagc cgcgtgcagc
     2941 tcggcgcgcg ctggcgcgac agcttcgtgt cgatgccggc ctggcgcttc atcctcgcgc
     3001 cgggagtcct gacgcagaac gcctgggcgg gcctgatggc gccgagcgtg gacgcagtcg
     3061 ggcgctattt cccgctcacc gtcgccgcgg cgctgcccgc ggcgagcgtc gatcaggtcg
     3121 ccacgctgct tgcggccgcc gcgtggttcg accaaatcga ggccatcgcg ctcaaggcaa
     3181 ttgcgcccgc cgccgacact gcagcgatcg atgccgcgct cgccgagcgg ccctttaaag
     3241 ccgcatggct gcagctgccg cgcgcgggcg accacgccat gccgatcaag ggcgcgaagt
     3301 cacagatgct ctgggttcct ttgcccgggc gcgctccgca cgagcgcccg ccggcaagcc
     3361 tgtacgagct cgctgagcgc ctggtcgagc cctattcggc atggctcgcc gaggagtcgg
     3421 aggtattcgg ccgctgcctg ctgctctgcg aggcgctgcc tgccgccggg cagtactgcg
     3481 ccatgatgga cgggcgctgg atcgagcatg gctggggccg ccgcaaaatc ggcgcctgac
     3541 gcgcgaccat gccaatcacc gtcaagatct tcaatcactc cgcggcccgg ccgacgcttt
     3601 acgcgtccca cgacttcgac cggccgccgg tgagcatcgg ccgcgacgcg gcatgcacca
     3661 tggtgctcga cgacccgcac aaacacattt cgcgctttca cgtcgagatc gaggaggagg
     3721 gcggtaacta ctggatgtcg gttgtctcca aggtgaaccc ggtcatggtc aagggccgtc
     3781 gctacggtcc gggcacgcgg ctgacgctga agagcggcga cactttcgag atcggcgaat
     3841 atgaaatcca cgtgatgctg tcggagggcc gcacgatgcc gggcgcggcg ccggccgggg
     3901 gagggtcgat cttcgaggag tcgaccttcg tcgccgggag ggcacccgcg gctgcggcga
     3961 gcgtcttcga ggagtcgacc ttcgtcgctg ggagggcacc cgcggctgcg gcgagcgtct
     4021 tcgaggagtc gaccctcgtc ggtgcagcga aggcacccgc gcctgcgccg cgcgtggcgc
     4081 ccgccgcccc cgccggggtg gtagccgacc tggtgggcgt gttcctcgaa ggcacaggcc
     4141 tgccgcacaa ggacctctct acagccgaag gcgagcggct gctgcgcgag tgcggcacga
     4201 tcctgcgcgc ggcaatcgag ggaatcatga tgctgctcat cgcgcgcgcc gagatgcgca
     4261 aggagttcca ggccgaggac cgcaccatgg tcgagacgcg cgacaacaat cccctgaagc
     4321 tcatgtccga cccgcacgag gcgatggcat tcctgttcga ccccgcggag cgcatcgagg
     4381 gctttctcga cccggtgcag gcggtgagcg acgcctgcga ggacctgcgc gcgcacgagc
     4441 tcgcgctgat ggcgggcatg cgcgccgcga tcctcggcgc gctgcggcgc ctcgatccgc
     4501 acgtcctcga gcgggcgttc gagaagtcct caggcggctt cagcctcgcg agccgcaagg
     4561 cgaggctgtg ggagctgttc gtcgcgcacc aggagaagct ctcgcacgac gcgcaggagg
     4621 atttcaacaa ggtgttcggg cgcgagttca tgggcgccta ccagtcgcag ctgcgccgcc
     4681 tgaggggagg gcgctagacg atcgttccga tactgtataa aattacagta tcgtggcccc
     4741 cctagaagat attctccagc gccatccggt ctggcgcggc acggcgccgg ggtgggccgc
     4801 gcccgtcgtg ccgacgggtt tcgccaatct ggatgcggag cttcccggcg gtggatggcc
//
LOCUS       DASPXW010000361         3982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_229345, whole genome shotgun sequence.
ACCESSION   DASPXW010000361 DASPXW010000000
VERSION     DASPXW010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229345"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VEL04_08320"
     CDS             complement(<1..271)
                     /locus_tag="VEL04_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HYT48412.1"
                     /translation="MEIFHNLLIGFQVAVHPYNVLYCFIGVILGTLIGVLPGIGPVAT
                     IAMLLPITFNLNPVSALIMLSGIYYGAQYGGSTTAILVNIPGESSS"
     gene            complement(283..741)
                     /locus_tag="VEL04_08325"
     CDS             complement(283..741)
                     /locus_tag="VEL04_08325"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HYT48413.1"
                     /translation="MKIKSPKDFWAGLMFIGSGLFFAIWAVAHYQMGTAVRMGPAYFP
                     VLLGGLIVVLGLVIFVGSFAFEGPAVPKFHFRPLILISLGCVLYGYLMKPLGLFVATA
                     ALVVVSAFGGHEFKWKEVLILYVILMLFSWLVFVKGLTLPFPVWPEFMGY"
     gene            817..1443
                     /locus_tag="VEL04_08330"
     CDS             817..1443
                     /locus_tag="VEL04_08330"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HYT48414.1"
                     /translation="MASLLPASAIRVVALMLIAALAAGCGERPKLERLPSDAVVLAFG
                     DSLTFGTGASEEESYPAQLEKLIGRRVVRAGVPGEVTGQALERLPAALDEHAPRLLLL
                     CIGGNDFLRRLGKQQAEDNVRAMVKLAKSRGVGVVLIGTPEPGLSVSPPAFYAGIAQE
                     FRLPYEEGIIGQVLKDSTLKADPIHPNARGYRVIAERIAEMMKKSGAI"
     gene            complement(1394..2041)
                     /locus_tag="VEL04_08335"
     CDS             complement(1394..2041)
                     /locus_tag="VEL04_08335"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HYT48415.1"
                     /translation="MIIVFARAPVPGQAKTRLVPRLGVWRAARLHARLTLHALRTALA
                     ARCGAVELHGTQRHAFFDRLGVVFRLQRGADLGERMYNALSRALRRHDTAILIGTDCP
                     MLAPQDLRRAARWLHGGWDAVLAPAEDGGYALVAMKRVRPELFEGIAWGGAQVYDETV
                     TRLSRAGYRWRALRRVWDVDRPEDLDRLRSLRFAGGAKRLRSLRFSSSFRRSARR"
     gene            complement(2038..2709)
                     /locus_tag="VEL04_08340"
     CDS             complement(2038..2709)
                     /locus_tag="VEL04_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HYT48416.1"
                     /translation="MVAQRLSVVIPALNEAGVIRAALEALAPLRVRGHEVIVADGGSE
                     DGTAQSAAALCDRVVLAARGRAAQMNAGAAAAGGDALVFLHADTRLPAHADELILDSL
                     QAHLWGRFDVEIDGRHRLLKAVACGMNLRSRLTGIATGDQAIFVRRAGFPGFPEIALM
                     EDIAFSTAMKRLGPPACRRETVLTSGRRWESRGVLRTILLMWRLRLMYFLGARPEALA
                     RIYDK"
     gene            complement(2670..3458)
                     /locus_tag="VEL04_08345"
     CDS             complement(2670..3458)
                     /locus_tag="VEL04_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HYT48417.1"
                     /translation="MDARLIVAAAVFLAAPVFSQSFDHAHKAWDGLLKRHVVVLDAGS
                     ASQLRYAGMAQDRAALKDYLAGLSAVGEAEFKGWTRPQQMAFLINAYNAFAVEKILTR
                     YPEIRSIWDFGKVFGNPFKDEFFFLLGRKGSLDWIEHQVLRKQYRDPRVHYAVNCASI
                     GCPMLREEAYVAERLERQLEEQARRFLADRSRNRFRDGRLEVSRIFDWYKGDFEPREQ
                     YFARYAKILTARQEDEQLVMQAKVPISFLDYDWSLNDFRSSSRR"
     gene            complement(3443..>3982)
                     /locus_tag="VEL04_08350"
     CDS             complement(3443..>3982)
                     /locus_tag="VEL04_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HYT48418.1"
                     /translation="PNIFACGDVAGPYQFTHTASHMAWYCAVNGLFGRFKKYRVDYSV
                     IPWATFTDPEVARVGLNELEARERNIPHEVTVYGIDDLDRAIAEGEAHGSVKVLTVPG
                     KDRILGATIVGEQAAELIVEFIAAMKHGIGLNKILGTIHIYPTLAEANKYAAGVWKKA
                     HAPQGLLRKVERFHAWMRG"
BASE COUNT          732 a         1329 c         1285 g          636 t
ORIGIN      
        1 ccgacgagga ttcgcccgga atgttcacga gtatcgcggt agtcgaaccg ccgtactgcg
       61 cgccgtagta gatcccggag agcatgatca gcgccgatac cgggttcagg ttgaaggtga
      121 tcggcagcag catcgcgatg gtcgcgaccg ggccgatgcc cggcagcacg ccgatcagcg
      181 tgccgagaat cacgccgatg aagcagtaca ggacgttgta cggatggacc gcgacctgga
      241 agccgatcag gaggttgtgg aagatttcca tcgcgcctcc gctcagtagc ccatgaattc
      301 gggccacacc gggaaaggca gcgtcaggcc tttcacgaac accagccatg aaaacagcat
      361 caagatcacg tacaggatga ggacctcttt ccatttgaac tcgtgaccgc cgaaggcgct
      421 gacgaccacc agcgccgccg tggcgacaaa cagcccgagc ggcttcatga ggtagccgta
      481 gagcacgcag ccgagcgaga tcaggatcag aggcctgaag tggaatttcg gcaccgccgg
      541 cccttcgaac gcgaaggagc cgacgaagat gaccagaccg agcaccacta tcagcccgcc
      601 gagcaggacc gggaaatagg ccggtcccat gcgcaccgca gtgcccatct ggtaatgcgc
      661 gactgcccag atcgcgaaaa acaggcccga gccgatgaac atcaggcccg cccagaagtc
      721 cttgggactc ttgattttca tgtctcctcc tacgccgctg gcagcggtct tgatgcggct
      781 ccccagcggc ggcaaagtgt aacatgcgct cccgcgatgg ccagcctgct ccccgcgtca
      841 gcaattcgcg tcgtcgccct catgctgatc gctgcgctcg cggcgggctg cggcgagcgc
      901 cccaagctgg agcgcctgcc gagcgatgcg gtggtcctcg ccttcggcga cagcctcacc
      961 ttcggcaccg gtgccagcga ggaagagagc tacccggcgc agctagagaa actgatcggg
     1021 cgccgcgtgg tgcgcgccgg tgtcccgggc gaggtcaccg gccaggcgct cgaacgcctg
     1081 ccggcggcgc tcgacgagca cgcgccgcgc ttgctgctgc tgtgcatcgg cggcaacgat
     1141 ttcctgcggc gcctcggcaa gcaacaggcc gaggacaacg tgcgcgcgat ggtgaagctg
     1201 gcgaagagcc gcggtgtcgg cgtggtgctg atcggcacgc ccgagcccgg cctcagcgta
     1261 tcgcctcccg ccttctatgc cggcatcgcg caggagttcc gcctgcccta cgaggaaggg
     1321 atcatcgggc aggtgctgaa ggattcgacc ctcaaggccg atccgatcca cccgaacgcg
     1381 cgcggctacc gcgtcatcgc cgagcggatc gccgaaatga tgaagaaaag cggagcgatc
     1441 tgagccgctt tgcgcctcct gcaaagcgga gcgatctgag ccggtccaga tcttcgggtc
     1501 gatcgacgtc ccacaccctg cgcagcgcgc gccagcggta tccggccctc gacagccgcg
     1561 tcaccgtttc gtcgtaaacc tgcgcgcctc cccatgcaat accttcaaac agctccggcc
     1621 tgacgcgctt catcgccacc agcgcgtaac cgccatcctc cgccggggcg agcaccgcgt
     1681 cccagccgcc atgcagccag cgcgccgcgc gccgcagatc ttgcggcgcg agcatcgggc
     1741 agtcggtgcc gatgaggatc gccgtgtcgt gacgccgtaa cgcgcgtgac aacgcgttat
     1801 acatgcgctc tcccaggtct gcgcctcgtt ggagacgaaa aaccacgccc aaccggtcaa
     1861 aaaacgcatg gcgctgcgtc ccgtgcaact ccactgctcc gcagcgggcg gcgagcgcgg
     1921 tgcgcagcgc gtgcagcgtc aggcgcgcgt gcaggcgtgc cgcgcgccat accccaagcc
     1981 gcgggaccag gcgcgtcttg gcctgcccgg gcaccggcgc tcttgcgaac acgatgatca
     2041 tttgtcgtag atgcgggcga gagcctcggg ccgggcgccg aggaaataca tcaggcgcag
     2101 gcgccacatg aggaggatgg tgcgcagcac gccgcgcgac tcccagcgcc gccccgaggt
     2161 gagcacggtc tcgcgcctgc aggcaggcgg cccgagccgt ttcatcgccg tgctgaaggc
     2221 gatgtcttcc atcagcgcga tctcggggaa gccgggaaag cccgcgcgcc gcacgaagat
     2281 cgcctggtcg ccggtggcga tcccggtgag gcgcgaacgc aggttcatgc cgcacgccac
     2341 cgccttgagc agccggtggc ggccgtcgat ctcgacgtcg aacctgcccc acaaatgggc
     2401 ttgcagggaa tcgagaataa gctcgtcggc atgcgccggc agccgggtgt cggcatgcag
     2461 aaaaaccagg gcatcgccgc cggccgcggc cgcgccggcg ttcatctgcg cggcccggcc
     2521 gcgtgcggcg agcaccaccc ggtcgcacag cgccgcggcg ctctgcgccg tgccgtcctc
     2581 gctgccgccg tcggcgacga tcacctcgtg gccgcggacg cgcagcggcg cgagcgcctc
     2641 gagcgcggcg cggatcacgc cggcttcgtt cagcgccggg atgacgaccg aaagtcgttg
     2701 agcgaccaat cgtagtcgag gaaggaaatg ggtacctttg cctgcatcac aagctgctca
     2761 tcttcctgcc gagcggtaag gatcttcgcg tagcgcgcga aatactgctc acgcggttcg
     2821 aagtccccct tgtaccagtc gaaaatcctc gacacctcga gcctgccgtc gcgaaagcgg
     2881 ttgcgcgagc ggtcggcgag aaatctcctc gcctgctcct cgagctggcg ctcgaggcgc
     2941 tcggccacgt aggcctcctc gcgcagcatc gggcagccga tcgaggcgca attcacggcg
     3001 taatgcacgc gcggatcacg atattgcttt ctgagaacct ggtgctcgat ccagtcgagg
     3061 ctgcctttgc ggcccagcag gaagaagaat tcgtccttga aaggattgcc gaacaccttg
     3121 ccgaaatccc agatcgagcg gatttcgggg tagcgcgtga ggatcttctc gacggcgaac
     3181 gcattgtagg cgttgatcag aaatgccatc tgctgcggcc tggtccagcc cttgaattcg
     3241 gcctcgccga ccgccgacag gccggcgaga tagtctttga gggcggcacg gtcctgcgcc
     3301 atgccggcat agcgcagctg cgacgcgctt ccggcatcga gaactaccac gtgcctctta
     3361 agcaatccat cccaggcttt atgcgcatgg tcgaaactct gggaaaaaac cggagccgcg
     3421 agaaaaaccg ctgccgcaac gatcagccgc gcatccatgc atggaacctt tcgactttgc
     3481 gcagcagccc ttgcggtgcg tgggccttct tccacacgcc cgcggcgtac ttgttggctt
     3541 cggcgagcgt gggatagata tggatggtgc cgaggatctt gttgagcccg atgccgtgct
     3601 tcatggcggc gatgaattcg acgatcagct cggccgcctg ctcgccgacg atcgtcgccc
     3661 cgagaatgcg gtccttgccc ggcacggtca gcactttcac gctgccgtgc gcctcccctt
     3721 cggcgatggc gcggtcgaga tcatcgatgc catagaccgt gacctcgtgc gggatgttgc
     3781 gctccctggc ttcgagctcg ttcaggccga cgcgcgccac ctcgggatcg gtgaacgtgg
     3841 cccacgggat caccgagtag tcgacgcgat acttcttgaa gcggccgaag agcccgttca
     3901 ccgcgcaata ccacgccatg tgggaagcgg tgtgggtgaa ctggtagggg ccggccacgt
     3961 cgccgcacgc gaagatgttg gg
//
LOCUS       DASPXW010000362         2981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_481045, whole genome shotgun sequence.
ACCESSION   DASPXW010000362 DASPXW010000000
VERSION     DASPXW010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481045"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="VEL04_08355"
     CDS             <1..695
                     /locus_tag="VEL04_08355"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYT48419.1"
                     /translation="LNQQLEERVSDQVAQLERLGRLKRFFSRPVAEAIVAGGEELLEP
                     HRREITAVFLDLRGFTAFTDRADPDQVLDLLRAYHATLGQRVDEFGGTLEHFAGDGIM
                     IFFNDPLPVENPVERAVRMSLALQQAFTPISTAWKKLGHDVGLGIGIAQGDATLGVIG
                     FENRWEYAAIGNVPNLAARLCGAARAGEILLDSQAHQDVAHLADTEFVGAITLRGFAQ
                     AVSTFRLKAIRS"
     gene            complement(762..1397)
                     /locus_tag="VEL04_08360"
     CDS             complement(762..1397)
                     /locus_tag="VEL04_08360"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HYT48420.1"
                     /translation="MKDRSAEILSKALLEEIAKVATVRPYPRNAIIVTEGDETDSLYV
                     MLSGKARVYVSDEKGREAALNQIGAGEYFGEVTLDGGPRSASVMALEECRCAVIRRAE
                     LTAFIERNPGFALHLVQKLAHRVRELTESVRSLALMDVYGRVARLLLELAEEKDGRLV
                     VAEPLTHKEIASRVGASREMISRIFSDLADGGYLRKEDGQLVIARKPPPRW"
     gene            1523..1708
                     /locus_tag="VEL04_08365"
     CDS             1523..1708
                     /locus_tag="VEL04_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48421.1"
                     /translation="MKITEYLLFLAVMVPTILILVAAVVSIAHPAPLPSASGPATTVS
                     SAGLYPANLPEEWDIRL"
     gene            1826..2749
                     /locus_tag="VEL04_08370"
     CDS             1826..2749
                     /locus_tag="VEL04_08370"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYT48422.1"
                     /translation="MAQPAQKLRRIGILDYGTAEPGRLDWWKAFRERLRELGYIEGQQ
                     VVYESRFAGGNAEQLRAFAAELVKLKVHLIVTAGNPAAAAANQATSEIPIVMATGTDP
                     VAVGLVKNLARPGGNLTGVTSITSVLSGKRVELLKVFVPGVSRVGLLLDESSPASRLA
                     ARETGAAAAALGVTVAAHDVGSPAKFDDTFKALRHERAQAIILISSATTFTQRKRLAD
                     LAVKHHLAAVTGSREYAQAGGLISYGTDYPHLFRRAAEYADKILKGAKPGDLPIELPS
                     KFELVVNLKTAKALGLKIPQSVLVRADEVIQ"
     gene            2746..>2981
                     /locus_tag="VEL04_08375"
     CDS             2746..>2981
                     /locus_tag="VEL04_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48423.1"
                     /translation="MKRRRALLLGLAGLALPFGPRAQPGGRIPRIGFLSLISPESDSR
                     LQSFLAGMRELGYVEGKTIVIEWRFTRGKTELLS"
BASE COUNT          477 a          993 c         1013 g          498 t
ORIGIN      
        1 agctgaacca gcagctcgag gagcgcgtca gcgaccaggt ggcgcagctc gagcgcctcg
       61 ggcgcctcaa gcgcttcttt tcgcgcccgg tggccgaggc gatcgtcgcc ggcggcgagg
      121 agctgctcga gccgcaccgg cgcgagatca ccgcggtgtt cctcgacctg cgcggcttca
      181 ccgccttcac cgaccgcgcc gacccggatc aggtgctgga cctgctgcgc gcttaccacg
      241 cgacgctggg gcaaagggtc gacgagttcg gcggcacgct cgagcacttt gccggcgacg
      301 gcatcatgat cttcttcaac gacccgctgc cggtcgagaa ccccgtggag cgcgccgtgc
      361 gcatgtcgct cgcgttgcag caggcgttca ccccgatctc gaccgcctgg aagaagctcg
      421 ggcacgacgt gggcctcgga atcggtatcg cgcagggcga cgccaccctc ggcgtgatcg
      481 gcttcgagaa tcgctgggag tacgcggcga tcggcaacgt gccaaatctt gcggcgcggc
      541 tctgtggagc cgcgcgcgcg ggtgaaatcc tgctcgactc gcaggcccat caagacgtgg
      601 cgcacctcgc cgacaccgag ttcgtcggcg cgatcaccct gcgcggcttc gcgcaggccg
      661 tgtccacgtt ccgcctgaag gcgatccgca gctagaaatc ggggtcagag cccgaatttt
      721 ggccccctga caagggggcg gcgagtgttg gcgagcgggg gttaccagcg cggcggcggc
      781 ttgcgggcga tgacgagctg gccgtcttct tttcggaggt agccgccgtc ggcgaggtcg
      841 gaaaagatgc ggctgatcat ctcgcgcgag gcgccgacgc gcgaggcgat ctccttgtgg
      901 gtgagcggct cggccaccac cagccggccg tccttctcct cggcgagctc gaggagcagc
      961 cgcgccacgc gcccatagac gtccatcagg gcgaggctgc gcacgctctc ggtgagctcg
     1021 cgcacccggt gcgcgagctt ctgcaccagg tgcagcgcga agcccggatt gcgctcgatg
     1081 aaggcagtga gctcggcgcg ccggatcacc gcgcagcggc actcctccag cgccatcacc
     1141 gaggccgaac gcggcccgcc gtcgagcgtc acctcgccga agtattcccc cgcgccgatc
     1201 tggttgaggg ccgcctcgcg ccctttctcg tccgacacat agacccgcgc cttgccagag
     1261 agcatgacgt acagcgaatc ggtctcgtcg ccctcggtga cgatgatggc gttcctgggg
     1321 taggggcgca cggtcgcgac cttggcgatc tcctcgagca gcgccttgga caggatctcg
     1381 gccgagcggt ctttcatgag cgcggattgt gccccggttc gccccgggag gtgaatttgt
     1441 cacactaatc gggggtcgtg tttcgctgac agagggtagc tgggaggcct accttcaatg
     1501 cgcatcccca tgggaggggc gcatgaaaat aaccgaatac ctgctgttcc tggctgtgat
     1561 ggtgccgacg atcctgattc tcgtcgccgc cgtcgtgtcg atcgctcatc ccgccccgct
     1621 gccttccgca agcgggccgg cgacgacggt ttcgtccgcg ggcctctacc ccgcgaacct
     1681 gcccgaggag tgggacatcc gcctctaggt gaatttttgc cccggccggg agatactcgc
     1741 gccggggagg agggacatgg agggaatcag gcgccggctg ctcgggcttc ttggcgcggc
     1801 gctcgccgcg gcgccgttca gcgctatggc ccagccggcg cagaagctgc gccgcatcgg
     1861 gatcctcgac tacggcaccg ctgagccggg ccgcctcgac tggtggaagg cattccggga
     1921 acgactgcgc gagctcggct atatcgaagg ccagcaggtg gtatacgaat cccgcttcgc
     1981 cgggggcaat gccgaacagc tgcgggcgtt cgccgctgag ctcgtcaagc tcaaggtgca
     2041 cctcatcgta accgcgggca atcctgccgc cgcggccgcc aaccaggcga cctccgagat
     2101 tcccattgtc atggcaacgg gaaccgatcc ggtggctgtg gggctcgtga aaaacctggc
     2161 aaggcctggt gggaatctca ccggcgttac ctcgatcacg agcgtattga gcggcaagcg
     2221 cgtggagctt ctaaaggttt tcgtgccggg cgtgtcgcga gtcggtcttc tgctcgacga
     2281 gagcagccct gcgtcgcggc tcgcggcaag agagaccggg gcggctgccg ccgcgcttgg
     2341 cgtgaccgtc gccgcgcacg acgtgggcag tcctgccaag ttcgacgaca cgttcaaggc
     2401 gctacgccat gagcgggccc aagcgatcat cctcatctcg agtgccacga ccttcacgca
     2461 acggaagcgg ctcgcggacc tggcggtgaa gcaccacctg gcagcggtca ccggcagccg
     2521 ggagtatgcc caggccggcg gtctcatcag ttacgggacc gactacccgc accttttccg
     2581 acgcgcggcc gagtatgcag acaagatcct caagggcgcg aagcccggcg acctcccgat
     2641 cgagctgccg agcaagttcg agctggtggt gaacctgaag accgccaagg cgctcggcct
     2701 caagattccg cagtcggtgc tggtgcgcgc cgacgaggtg atccagtgaa gcggcgccgc
     2761 gcgctgctgc tggggcttgc tgggcttgcg ttgcccttcg gaccgcgcgc ccagccaggg
     2821 ggccgcattc cgcgaatcgg ctttctcagc cttatatcgc cggagtccga ctcgcggttg
     2881 cagtcattcc tcgccggaat gcgcgagctc ggttacgtcg agggcaaaac gatcgtgatc
     2941 gaatggcgct tcacgcgcgg caagacggag ctgctttccg a
//
LOCUS       DASPXW010000363         6115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_611181, whole genome shotgun sequence.
ACCESSION   DASPXW010000363 DASPXW010000000
VERSION     DASPXW010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6115
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611181"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="VEL04_08380"
     CDS             <1..704
                     /locus_tag="VEL04_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HYT48424.1"
                     /translation="RAKGAEGLQSPIVKNLHARSLSEILKRTGAEDGDLLFFGADTEA
                     TVNTCMGELRQKLGHEHGLAEKGWRPLWVVDFPMFELDAETRQWKARHHPFTSPKDGH
                     EDHIESAPGKAYAKAYDVVLNGWEIGGGSVRIHREEIQQKVFRALRISPEEQRAKFGF
                     LLDALQYGAPPHGGIAFGFDRMVALMAGAEAIRDVIAFPKTQRGQDLLTGAPTPVTEK
                     QLRELHIRLRKPPQS"
     gene            715..1047
                     /locus_tag="VEL04_08385"
     CDS             715..1047
                     /locus_tag="VEL04_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HYT48425.1"
                     /translation="MRFWLAVAVTAIAALARADAGQITLGELPAEASQTLRLIRQNGP
                     FPFQRDGAVFVNREGRLPSRSRGYYREYTVKTPGAHERGARRIIAGRGGEYYYSDDHY
                     RSFRRIIE"
     gene            1047..1463
                     /locus_tag="VEL04_08390"
     CDS             1047..1463
                     /locus_tag="VEL04_08390"
                     /inference="COORDINATES: protein motif:HMM:NF013500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HYT48426.1"
                     /translation="MGKLLERLRDPSRSGVYRASRTDVIADAVRGSALDVAEISLAKE
                     HLFKAFASALEFPAWFGQNWDALEDCLGDLSWRAAEGHVLLVLHHDRLPKDDLGVLLD
                     VLASSAEYWAARGKPFFAVFIDPRRSLELPDLYREK"
     gene            1460..1918
                     /gene="nudB"
                     /locus_tag="VEL04_08395"
     CDS             1460..1918
                     /gene="nudB"
                     /locus_tag="VEL04_08395"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HYT48427.1"
                     /translation="MSTRKFKLPFSVLVVVHTAEREVLLLERAARPGYWQSVTGSLDQ
                     PDEPLELTAAREVREETGIDVAQGRLARWNVAHTFEIYTQWRHRFAPGVTHNTEHVFG
                     LELAARLPVTLSGAEHTAFAWLPWHEAAGKCFSWSNRDAIRMLGERGVIT"
     gene            1924..3429
                     /locus_tag="VEL04_08400"
     CDS             1924..3429
                     /locus_tag="VEL04_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48428.1"
                     /translation="MRVIPGAAAVLLAAGCATFEGQFAAHLASPSAEVRECAGWYRAL
                     DEQVEAAGVRDAQYARVSGFPYLRVDRFLASSGGKVGDRTAAWQAFIERLRSLDLESR
                     RHEIDNLPRSQFDALPAMGADITRAYALARTRECGGRLAGVDLADPRARAELLAAARV
                     PDEYSSAQRLVGLYALTRIPFADGVKRWQDEMLALFRGEAPTPPGASRVRFAPPTGKI
                     LPRSTVLGLLARAQFDPLGQPMLSEREFELIAAAYAPSFDIAIAGDYDRFGALRWRRE
                     GALPEVDAAEPVVYVQPAYTRYGERVLLQVVYTLWFPERPPRGAVDLLAGRLDGLVWR
                     VTLAPDGEPLLYDSMHPCGCYHQFFPTPRARPRPAPDALEEWAFVPQSLPGVHDGEQP
                     VVSIASATHYIEGVAVAPGAASLVRYAFRPYDELRSLGRLDGGRRSAFGADGLIAGSE
                     RAERFLYWPMGIPSPGAMRQWGRHATAFVGRRHFDDADLIERRFALDLGAE"
     gene            3426..4175
                     /locus_tag="VEL04_08405"
     CDS             3426..4175
                     /locus_tag="VEL04_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HYT48429.1"
                     /translation="MTERLRVVTLNIHKGLSQFNRRMVIHGLREGLRALDPDLVFLQE
                     VQGLNQRFALRYAASPPEAQHEFLAGDRWQHVYGRNRVYDHGHYGNAILSRFPFVSSE
                     NEDVSDHRFERRGLLHCVVSVPRWRRNLHCVCVHLSLHEGGRSRQLEAISGRLEELAS
                     RGLPIIIAGDFNDWRHRASAILERRLGMSEVSLARHGRAARTYPSMLPVLRLDRIYVR
                     GFKIAASQVHRGKPWSRLSDHLAVSAELERA"
     gene            4180..5331
                     /gene="clsB"
                     /locus_tag="VEL04_08410"
     CDS             4180..5331
                     /gene="clsB"
                     /locus_tag="VEL04_08410"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HYT48430.1"
                     /translation="MQYVGGNRLTLLRNGERYFPALVAAIDAAQNEVFLESYIYADDQ
                     TGSLLTDTLARAAARGARVRLLLDGFGARDFPRRWRELLRAAGAELLVFRPEVASLRL
                     RRDRLRRMHRKLASIDGRTAFVGGINIIDDFATPAERRPRYDYAVRIEGPLAAEVRAA
                     AARLWLRVSWAAMRRRARVPPAPPPGVPAGDQRAALVVRDSLRHRRDIENAYLHYIGG
                     AREEIIIACAYFFPGRRFRRAIVVAAARGVRVRLLLQGMVEYPLVHYASRALFGVLLD
                     AGVEIHEYHRSILHAKVAVFDARVACVGSSNIDPFSLLLAREANVFVDDRGFAGELRA
                     SLEEAMRVGATPVPARRWREQPLLLRLRIWIDYGIVRLLISLFGLERYH"
     gene            complement(5300..6070)
                     /locus_tag="VEL04_08415"
     CDS             complement(5300..6070)
                     /locus_tag="VEL04_08415"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HYT48431.1"
                     /translation="MSARIELNHRIHVLTKKEELDTVRVQGKVVVVIDVLFATTTMVS
                     ALAHGAAEVVPVIDESAARVYSRDLIENSFVLSGELYADTLPGFAHPAPLALLEHGVG
                     GKTVIYSTTNGTVAMTQAAAAARVYCGALLNARRLAEHIAAEHPRETVLLLCSGSGSN
                     FNLEDFYAAGCLVEHLAGLAGGAADLSDAALAARVLFRSMPASDALLECRVGRMMVAR
                     GLRREVEFACRLDGLPVVAALEQGRVRLVSDSARARTS"
BASE COUNT          902 a         2098 c         2124 g          991 t
ORIGIN      
        1 agcgggcgaa aggcgccgaa ggattgcagt cgccgatcgt gaagaacctg catgcgcgct
       61 ctctctccga aatattgaaa cgtaccggcg ccgaagacgg ggatctgctt ttttttggcg
      121 ccgataccga agcgacagtg aacacctgca tgggtgagtt acgtcaaaag ctcggccacg
      181 agcacgggct ggcggagaaa ggctggcgcc cgctctgggt ggtcgatttc ccgatgttcg
      241 agctcgacgc cgagacgcgg cagtggaaag cacgccatca tccgttcacc agcccgaagg
      301 acggtcacga ggaccacatc gagagcgcgc ccgggaaggc ctatgccaag gcctacgacg
      361 tggtgctcaa cggctgggaa atcggcggcg gctcggtgcg catccatcgc gaagagatac
      421 agcagaaggt gtttcgcgcc ctgagaattt ctcccgagga gcagcgcgcg aagttcggct
      481 tcctgctcga tgcgctgcaa tacggcgcgc cgccgcacgg cggcatcgcc ttcggcttcg
      541 accgcatggt ggcgctgatg gcgggcgccg aagcgatccg ggacgtcatt gccttcccca
      601 agacccagcg cggccaggac ctgctcaccg gcgcgccgac cccggtcacc gaaaagcagc
      661 tgcgcgagct gcatatacgg ctgaggaagc cgccgcagtc gtagagttgc gggggtgaga
      721 ttctggctcg ccgttgcagt gaccgcgatc gcggcgctcg cgcgtgcgga cgcaggacag
      781 attacgctcg gcgagcttcc ggcggaggcc agccagactc taaggctgat caggcagaat
      841 ggtccatttc cgtttcagcg ggacggcgcc gtgttcgtga atcgtgaggg ccggctgcca
      901 agccgcagcc gcgggtatta ccgcgaatac acggtgaaga cgcccggggc gcacgagcgc
      961 ggcgcgcggc gcatcatcgc cgggcgcggc ggcgagtact actacagcga tgaccactac
     1021 cgcagcttca ggcggatcat cgagtaatgg gcaagcttct cgaacggctc cgcgacccct
     1081 cgcgctcagg cgtctaccgc gcgtcccgga ccgatgtaat tgccgatgcg gtgcgtggca
     1141 gcgcgctcga tgtcgcggaa ataagtcttg caaaggagca tctgttcaag gcgtttgctt
     1201 cagcgctgga gttccccgca tggttcgggc agaactggga tgcgctcgag gattgcctcg
     1261 gcgacctgtc ctggcgcgcg gccgaaggcc atgtattgct ggtgctgcac cacgaccggc
     1321 tccccaagga cgacctgggg gtgttgctcg atgtgctcgc ctcgagcgcg gagtattggg
     1381 ccgcgcgcgg caagccgttt ttcgccgtgt tcatcgatcc gcggcgctcg ctcgagctgc
     1441 ccgatctgta ccgggagaaa tgagcacgcg aaagttcaag ctgcccttct cggtgctggt
     1501 cgtggtgcac acggccgagc gggaggtgct gctcctcgag cgcgcggcgc gcccgggtta
     1561 ctggcagtcg gtgaccggct ctctcgatca gcctgacgag ccgctcgagc tcaccgccgc
     1621 gagagaggtg cgtgaagaga ccggcatcga cgtcgcgcag ggcaggctcg cgcgctggaa
     1681 cgtggcgcat actttcgaga tctacacgca gtggcgccac cgcttcgctc ccggcgtgac
     1741 gcacaatacc gagcacgtgt tcggcctcga gctcgcggcg cggcttccgg tgacgctgtc
     1801 cggggccgag cacaccgcgt tcgcctggct gccgtggcac gaagccgccg ggaaatgctt
     1861 ctcctggtcg aaccgcgacg cgatccgcat gctcggcgag cgtggtgtga taacttagac
     1921 gcggtgcgag tcattcctgg tgcggccgcc gtgctgctcg cggccggttg cgcgacattc
     1981 gaagggcagt tcgcggctca tctcgcatcg ccttcggccg aggtgcggga atgcgccggc
     2041 tggtatcgcg ccctcgacga gcaggtcgaa gccgccggcg tgcgcgacgc ccagtacgcc
     2101 cgcgtgagcg gttttccgta cctgcgcgtg gatcgctttc tggcttcgtc aggcggcaaa
     2161 gtgggcgata gaacggccgc ctggcaggcc ttcatcgagc ggcttcgcag cctcgatctc
     2221 gaatcgcgcc ggcatgaaat cgacaacttg ccgcgcagcc aattcgatgc gcttcccgca
     2281 atgggggcgg atatcacccg ggcctacgcg ctcgcgcgca cgcgcgaatg cggcggccgg
     2341 ctcgcggggg tcgatctcgc cgatccacgc gcgcgcgccg agctgctcgc cgccgcgcgg
     2401 gtgccggacg aatattccag cgcgcagcgc ctggtcgggc tttatgccct cacgcgcatc
     2461 ccgttcgccg atggcgtcaa gcgctggcag gacgagatgc tggctttgtt ccgcggcgag
     2521 gcgccgacgc cgccgggcgc cagccgggtg cggtttgcgc cgcccaccgg aaagattctg
     2581 ccgcgcagca cggtgctcgg cctgcttgcg cgcgcgcagt tcgacccgct cggccagccc
     2641 atgctgtcgg agcgcgagtt cgagctgatc gcggcagcct acgcgccgag cttcgacatc
     2701 gcgatcgccg gcgactacga ccgcttcggc gccttgcgct ggcggcgcga gggcgcactg
     2761 cccgaggtcg acgcggccga gcccgtcgtc tacgtgcagc ctgcctatac gcgctacggc
     2821 gagcgcgtgc tgctgcaagt cgtctacacg ctctggtttc cggaacgccc gccgcgcggc
     2881 gcggtcgatc tgcttgcggg gcggctcgac ggtctggtct ggcgcgtcac gcttgccccg
     2941 gacggcgaac ctctcctcta tgactcgatg catccctgcg gctgctatca ccagttcttc
     3001 ccgacgccgc gggcgaggcc gcggccggcg cccgatgcgc tcgaggagtg ggcgttcgtg
     3061 ccgcaaagcc tgcccggcgt gcacgatggc gagcagccgg tggtgagcat cgcctcggcc
     3121 acgcattaca tcgagggcgt ggccgtcgcg cccggtgcgg cgagcctggt gcgctacgcg
     3181 ttccggcctt acgacgagct gcgctcgctc ggccggctcg acggcggccg gcgcagcgcc
     3241 ttcggcgccg acggcctgat cgccggcagc gagcgcgccg agcgtttcct ctactggccg
     3301 atgggaatcc cttcgcccgg ggccatgcgc cagtgggggc ggcacgccac cgccttcgtc
     3361 gggcgccggc atttcgacga cgccgacctg atcgagcggc gctttgcgct cgacctgggg
     3421 gctgagtgac cgagcggctg cgcgtcgtca cgctgaacat ccacaagggg ctgtcgcagt
     3481 tcaaccgccg catggtgatc cacggcctgc gcgagggcct gcgcgcgctc gatccggacc
     3541 tggtgttcct gcaggaagtg caggggctca accagcgctt cgctctgcgc tatgccgcct
     3601 ccccgccgga ggcgcagcac gagttcctcg ccggcgatcg ctggcagcac gtgtacggcc
     3661 gcaatagggt ctacgaccac ggccactacg gcaacgccat cctgtcgcgc tttccgttcg
     3721 tctcgtccga gaacgaggac gtgtcggacc accgcttcga gcgccgcggg ctgctgcact
     3781 gcgtggtgtc ggtgccgcgc tggcggcgca acctgcactg cgtctgcgtg cacctgtcgc
     3841 tgcacgaggg cggccgcagc cgccagctcg aagcgatctc gggccgcctc gaagagctcg
     3901 cctcgcgcgg cctgccgatc atcattgcgg gcgacttcaa cgactggcgc caccgcgcat
     3961 ccgcgatcct cgagcggcgg ctcgggatga gcgaagtctc gctcgcgcgg cacggccggg
     4021 cggcgcgcac ctacccgagc atgctgccgg tgctgcggct cgaccggatc tacgtgcgcg
     4081 gcttcaagat cgccgcttcg caggtgcacc gcggcaagcc gtggtcgagg ctctcggacc
     4141 atctggccgt aagcgcagag ctcgagcgcg cctgaaccga tgcagtatgt cggcggcaac
     4201 cggctcacgc tcctgaggaa cggcgagcgg tatttccccg cgctggtcgc ggcgatcgac
     4261 gccgcgcaga acgaagtttt cctcgagagc tacatctacg ccgacgacca gaccggcagc
     4321 ctcttgaccg acacgctggc gcgcgccgcg gcgcgcgggg cgcgcgtgcg gctgctgctg
     4381 gacggattcg gcgcgcgcga ctttccgcgg cgctggcgag agctgctgcg cgccgccggt
     4441 gccgagctgc tcgtgttccg cccggaggtg gcgtcgctgc gcctgcgccg cgaccgcctg
     4501 cgccgcatgc accgcaagct cgcgtcgatc gacggccgca ccgccttcgt cggcggcatc
     4561 aacatcatcg acgacttcgc cacccccgca gagcggcgcc cgcgctacga ctacgcggtg
     4621 cgcatcgagg ggccgctcgc ggccgaggtg agagccgccg cggcgcgcct ttggctgcgg
     4681 gtgtcgtggg cggcgatgcg ccgccgggcg cgcgttccac cggcgccccc gccgggggtg
     4741 ccggccggtg accagcgcgc cgcgctggtg gtgcgcgaca gcctgcgcca tcgccgcgac
     4801 atcgagaacg cctacctgca ttacataggc ggcgcgcgcg aggagatcat cattgcctgt
     4861 gcctatttct ttcccgggcg caggtttcgc cgcgcaatcg tcgtcgccgc ggcgcgcggc
     4921 gtgcgcgtgc ggctgctgct gcaggggatg gtggaatatc cgctggtgca ttacgcctcg
     4981 cgcgccctgt tcggtgtgct gctcgacgcc ggtgtcgaga tccacgaata tcaccgcagc
     5041 attctgcacg ccaaggtggc ggtgttcgac gcccgcgtgg cctgcgtggg ttcgtcgaac
     5101 atcgacccgt tcagcctgct gctcgcgcgc gaggcgaacg ttttcgtcga cgaccgcggc
     5161 ttcgccgggg agctgcgcgc gagcctcgag gaggcgatgc gcgtcggggc gacgccagtg
     5221 ccggcgcgcc ggtggcgcga gcagcccctg ctgctgcggc tgcggatctg gatcgactac
     5281 ggcatcgtgc ggctgctcat cagcttgttc gggctcgagc gctatcactg acgaggcgca
     5341 cgcgcccctg ctcgagcgcg gccaccaccg gcaggccgtc gagccggcag gcaaactcga
     5401 cttcgcggcg cagcccgcgc gccaccatca tccgtcccac gcggcactcg agcagcgcat
     5461 cgctggcagg catgctgcgg aacagcacgc gtgctgccag cgccgcgtcc gatagatcgg
     5521 cggcgccgcc ggcgagcccg gcgaggtgct cgaccaggca gcccgcagcg tagaaatcct
     5581 cgaggttgaa gttgctgccc gagccggagc acagcagcag cacggtctcg cgcggatgct
     5641 cggcggcgat gtgctcggca aggcgccgcg cgttgagcag cgcgccgcag tacacgcgcg
     5701 cggccgcggc ggcctgcgtc atcgccacgg taccattggt cgtggagtag atcacggtct
     5761 tgccgccgac gccgtgctcg agcagcgcga gcggcgcagg gtgcgcgaac cccggcagcg
     5821 tgtcggcata gagctcgccc gacaggacaa acgaattttc aattaggtct cttgaataga
     5881 cccgggcagc ggactcgtcg atcaccggca ccacctcggc ggcgccgtgc gcgagcgcgc
     5941 tcaccatggt ggtggtagcg aacagcacgt caatcaccac gaccaccttg ccttgcacgc
     6001 gcaccgtatc gagctcctcc ttcttcgtga ggacatgaat cctgtggttg agctcgatgc
     6061 gcgcgctcat gacagcggcg cgactcgctc atagcgccgg atcctttcgc gcagc
//
LOCUS       DASPXW010000364         6522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_109090, whole genome shotgun sequence.
ACCESSION   DASPXW010000364 DASPXW010000000
VERSION     DASPXW010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6522
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109090"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..706
                     /gene="lolA"
                     /locus_tag="VEL04_08420"
     CDS             98..706
                     /gene="lolA"
                     /locus_tag="VEL04_08420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HYT48432.1"
                     /translation="MRCLVLLTILFVSATQAASTDRFKSFLRTTQTARGAFEQKVYDR
                     GGKLVQESKGSFVFLRPGRFRWVYDKPVDQVIVGDGERVWIYDRGLNQVTVRKLTRAL
                     GATPAALLAGSSDIDAAFELSDAGSRDGLEWLDARPREPEAGFERVRMGFDAAGVQAM
                     ELSDNFGQTTLLKFSSLERNPKVDAKEFRFEPPKGTDVLGDR"
     gene            703..1989
                     /locus_tag="VEL04_08425"
     CDS             703..1989
                     /locus_tag="VEL04_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HYT48433.1"
                     /translation="MNDLFGKREPAAPLAEALRPKTIDQVVGQQHLLGPGKPLRLAFA
                     SGKPHSMILWGPPGTGKTTIARLMAKAFDAEFIAISAVFSGVKDIREALQAAENELAR
                     SGRHTILFVDEVHRFNKAQQDAFLPYVERGLITFVGATTENPSFEVISALLSRATVYV
                     LEPLNEQDLGILLQRTNLKVDDEAKARLIGFADGDARRLLNAVEILMSTGAETVDGAF
                     VEQTLAKNLRRFDKGGEQFYDQISALHKSVRGSDADAALYWLVRMLDGGADALYIGRR
                     VVRMAVEDVGLADPRALRIALDACETYERLGSPEGELALAEAVIYLALAAKSNAIYEA
                     YNAARDFVSSDGTRPVPLHIRNAPTRLMKDLGYGKNYRYAHDEEGAYAAGESYFPEGM
                     PAVEWYRPTDRGLEAKIREKLAELRSRDQAAGKKKK"
     gene            1986..3269
                     /gene="serS"
                     /locus_tag="VEL04_08430"
     CDS             1986..3269
                     /gene="serS"
                     /locus_tag="VEL04_08430"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HYT48434.1"
                     /translation="MIDIQLLRSNLDAVAARLATRGGYALDVARFRELEGRRKELQTL
                     VEKLQAERNQIAKQIGQAKAQGEDASQLLKKAELGKASLGASEQQLAEIQEEFQQFIA
                     GIPNLPHASVPTGKSSDDNPERRRHGMPRKFAFPVKDHVDVGEGLAGLDFAAATKIAG
                     ARFVMMRGAVAHLHRALAQFMLDVHAREHGYTEIHTPYLVNAATAYGTGQLPKFEQDL
                     FLVPRGEEKWYLIPTAEVPVTNIVRDEIVAPEKLPLRFVCHSPCFRSEAGSYGKDTRG
                     MIRNHQFDKVELVQVVHPARSYQALEELTGHAEVILKRLELPYRVVELCTADIGAASA
                     KTYDLEVWLPSQNAYREISSCSNCEAYQARRMQARFRNEKGKPELVHTLNGSALAVGR
                     TLVAVLENYQNADGSVAVPAVLRPYMNDAEKLARP"
     gene            3287..3376
                     /locus_tag="VEL04_08435"
     tRNA            3287..3376
                     /locus_tag="VEL04_08435"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3321..3323,aa:Ser,seq:cga)
     gene            complement(3422..5455)
                     /gene="pbpC"
                     /locus_tag="VEL04_08440"
     CDS             complement(3422..5455)
                     /gene="pbpC"
                     /locus_tag="VEL04_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HYT48435.1"
                     /translation="MKKLLGTSAGFLFAAGFVASLFVEPYDVVPYSEVKSEWRSSDAW
                     LLDRNGEPLSRIRIDKTSRRGDWTALRDVSPALIVAVLAAEDRRFLGHAGVDWLALPG
                     ALRDQRGGSTITMQLAAWLHPELARSGSRGLVHKWRQMRQAMAMERRWNKDELLEAWL
                     NLTPFRGELEGIDAASRSLFAKRPDGLDRAESALLAALVRAPNAASARVTRRACALVR
                     PDDAACREIQWLALTGLKAAGVSRRLEGDAPHLARRLLTTPGERRRSTLDARLQRFAA
                     ETLERHLRELDGRNVEDGAALVLDNASGDILAWVGSSGELSSAAQVDGVTAKRLPGST
                     LKPFLYALAIERRLLTAASLLDDTPTAITTPSGLYVPQNYDRSFRGKVSLRTALAGSL
                     NVPAVRTLALVGYEPFHRRLREVGFSLERDADHYGYALGLGGAEVTLLELTRGYRRLA
                     RFEGFDERAAYIVGDILADAAARATTFGLANPLATRYAASVKTGTSTDMRDNWAVGYS
                     ARYTVGVWVGNFSGEPMHDVSGVSGAAPVWREIMDYLHEGAPAAPPAAPTGLIRKKIS
                     YANAAEPPRDEWFLAGTETALIVPLEAPARRPRIVSPANGAILAIDPDIPMAYQRVRI
                     EVSGAGADTRLMLDDGSIAPQLWLPPPGRHVLRLVSASGAELDRVQVTVRGLR"
     gene            complement(5452..>6522)
                     /locus_tag="VEL04_08445"
     CDS             complement(5452..>6522)
                     /locus_tag="VEL04_08445"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HYT48436.1"
                     /translation="VRKLAAIEALARHGRATPQMLESITIDPPLWPTSALIDWIGILQ
                     RVAGVPDGELRLHEALSQLRARLNFQGTAMTFSTERTDALWWLMVSADVNANRALLTV
                     LDVEDWREDIGRMVRGALFRQKRGIWGTTVANAWGVVATGQFSGRFEKTPAAGSVEAQ
                     LGQSAVSVSVSHEKQLRDIAWPAGREMLNLTHTGKGAPWAIVQSRAAIPLKAPLSSGY
                     AIRRTITPVEQKEKSIYSRGDVYRVTLEVDAQTDMTWVVIDDPIPAGAAILGSGLGRD
                     AASFTAGEKRAGWAWPVFIERSHDAYRAYYEFVPKGRFKIEYTVRLNNPGRFDLPATR
                     VEAMYLPEMFGELPNAGVQVRP"
BASE COUNT         1135 a         2156 c         2190 g         1041 t
ORIGIN      
        1 gatcgaggac atggagcgcg ccggcatggt ttcggcaatg cagtcgaacg gcaaccgcga
       61 ggtgctggta cccgcgaagg cggagtaggc cgccgcgatg cgctgccttg ttttactgac
      121 aatattattt gtatcagcga cgcaggccgc aagcaccgat cgcttcaaga gcttcctgcg
      181 caccacgcag acggcgcgcg gcgccttcga gcagaaggtc tacgaccgcg gcggcaagct
      241 ggtgcaggag tcgaagggca gcttcgtatt cctgcggccg gggcggtttc gctgggtgta
      301 tgacaagccg gtcgaccagg tgatcgtcgg cgacggcgag cgggtctgga tctacgaccg
      361 cggcctcaac caggtgacgg tgcgcaagct gacgcgcgcc ctcggcgcga cgcccgcggc
      421 gctgctcgcc ggctcctccg acatcgacgc ggcgttcgag ctctccgacg cgggcagccg
      481 cgacggcctc gaatggctcg acgccagacc gcgcgagccc gaggccggct tcgagcgcgt
      541 gcgcatggga ttcgacgccg cgggcgtgca ggcgatggag ctcagcgaca acttcggcca
      601 gaccacgctg ctgaaattct cctcgctcga gcgcaacccg aaggtcgatg cgaaggaatt
      661 ccgtttcgaa cctccaaagg gcacggacgt cctaggggat cggtgaacga ccttttcggt
      721 aaacgcgaac cggcggcgcc gctagcggaa gctctacgtc cgaaaaccat cgaccaggtc
      781 gtcgggcagc agcacctgct cggtcccggc aagccgctgc gcctcgcgtt cgcgtcgggc
      841 aagccgcact cgatgatcct gtgggggccg ccgggcaccg gcaagaccac catcgcacgc
      901 ctcatggcga aggccttcga cgccgagttc atcgccatct ccgcggtgtt ctccggcgtg
      961 aaggacatcc gcgaggcgct gcaggcggcc gagaacgagc tggcgcgctc cgggcgccac
     1021 acgatcctct tcgtcgacga ggtgcaccgc ttcaacaagg cgcagcagga cgccttcctg
     1081 ccctacgtcg agcgcggcct gatcactttc gtcggcgcga ccaccgagaa cccctctttc
     1141 gaggtgatca gcgcgctgct ctcgcgggct acggtatacg tgctcgagcc gctcaacgaa
     1201 caagatttgg gaattttgct tcaaagaacg aacctcaagg tcgacgacga agcaaaggca
     1261 cgcctgatcg gcttcgcaga cggcgacgcg cgccggctgc tgaatgccgt ggagattctc
     1321 atgagcaccg gcgccgagac ggtcgacggc gccttcgtcg agcagacgct ggcgaagaac
     1381 ctgaggcgct tcgacaaggg tggcgagcag ttctacgacc agatctcggc gctgcacaag
     1441 tcggtgcgcg gctcggacgc cgacgcggcg ctctactggc tggtgcgcat gctcgacggc
     1501 ggcgccgatg cgctctatat cggccggcgc gtggtgcgca tggcggtcga ggacgtcggg
     1561 ctcgccgatc cgcgcgcgct gcgcatcgcg ctcgacgcct gcgagaccta cgagcgcctc
     1621 ggcagccccg aaggcgagct ggcgctcgca gaggcggtga tctatctcgc gctggcggcg
     1681 aagtcgaacg cgatctacga ggcctataat gccgcgcgcg atttcgtctc cagcgacggc
     1741 acgcgcccgg tcccgctgca catcaggaac gcaccgaccc gcctcatgaa agatctcggc
     1801 tacggcaaga attaccgtta cgcgcacgac gaagagggtg cgtacgccgc gggcgagagc
     1861 tactttcccg aaggcatgcc cgcggtcgaa tggtaccggc cgaccgatcg cggcctcgag
     1921 gcgaagatcc gcgagaagct cgccgagctg cgcagccgcg accaggccgc aggaaagaag
     1981 aagaaatgat cgacatccag ctgctgcgct cgaacctcga cgccgttgcc gcgaggctgg
     2041 cgacgcgcgg tggctacgct ctcgatgttg cccggttccg cgagctggag ggacgccgaa
     2101 aggaactcca gacactggtg gaaaagctcc aggcggagcg caaccagata gcgaagcaaa
     2161 tcggccaggc aaaagcgcag ggcgaggatg cgtcgcagct gctgaaaaag gccgaacttg
     2221 gcaaggcctc gctgggagct tctgagcagc agctggcgga gatccaggaa gagtttcagc
     2281 aattcatcgc cgggattccc aatttgcctc acgcctcggt tccgaccggc aagtcttccg
     2341 acgacaaccc cgaacggcgc cggcacggca tgccgcgcaa gtttgcattc ccggtgaagg
     2401 accatgtcga tgtcggggag ggactggccg gccttgactt cgccgctgcg accaagatcg
     2461 ccggggcgcg ttttgtcatg atgcgcggcg cggtggcgca cctgcaccgc gcgctcgcgc
     2521 agttcatgct cgacgtgcat gcgcgtgagc acggctatac cgagatccat acgccttacc
     2581 tggtgaacgc ggccactgcc tatggcaccg gccagctgcc gaaattcgag caggacctgt
     2641 tcctcgtgcc gcgcggcgag gagaagtggt acctgattcc caccgccgaa gtgccggtga
     2701 ccaatatcgt gcgcgacgag atcgttgcgc ccgagaagct gccgctgcgc ttcgtctgcc
     2761 actcgccgtg ctttcgttcc gaggccggct cctacggcaa ggacacgcgc ggcatgatcc
     2821 gcaaccacca gttcgacaag gtcgagctgg tccaggtggt gcacccggcg aggtcctatc
     2881 aggcgctcga ggagctcacc ggccatgccg aagtcatcct gaagaggctc gagctgcctt
     2941 accgcgtggt ggagctgtgc accgccgaca tcggcgcagc ttccgcgaag acctacgatc
     3001 tcgaggtctg gctgccgtcg cagaacgcct accgcgagat cagctcgtgc tccaactgcg
     3061 aggcctatca ggcgcggcgc atgcaagcgc gcttccgcaa tgaaaaaggc aagcccgagc
     3121 tggtgcacac gctgaacggt tcggcactcg ccgtcgggcg cacgctggtc gcggtcctcg
     3181 agaattacca aaatgcggac ggctcggtgg cggttcctgc agtgctccgt ccgtacatga
     3241 acgacgccga gaagctcgcg cgcccctgat agaatccgcc ccctgcggag agatggcaga
     3301 gtggtctaat gcggcagact cgaaatctgt tgtacggtta tccgtaccgg gggttcgaat
     3361 ccctctctct ccgccaacca gtccagtgcg aaccggcggc gcactccttg ggccttttta
     3421 gtcagcgcag tccgcgtacc gtcacttgca cgcggtcgag ttcggcgccc gatgcgctga
     3481 ccaggcgtag cacgtggcgc cccggcgggg gcagccagag ttgcggggca atgctgccgt
     3541 cgtcgagcat caatcgtgta tcggccccag cgccggatac ttcgattctg actctttggt
     3601 acgccatcgg aatgtcggga tcgatcgcga ggatggcgcc gttggcggga gagacgatgc
     3661 gcgggcgtcg ggcgggcgcc tcgagcggca cgatgagcgc ggtctcggta ccagcaagga
     3721 accattcgtc tctaggcggt tccgccgcgt tcgcatagga gattttcttg cgaattaggc
     3781 cggtgggggc agccggcggc gcggctggcg cgccctcgtg caggtagtcc atgatttcgc
     3841 gccaaaccgg ggccgcgccc gagacgccgg aaacgtcgtg catcggctct ccggagaagt
     3901 tgccaaccca gacgcccacc gtatagcgcg ccgagtaacc gaccgcccag ttatcgcgca
     3961 tgtcggtgct ggtgccagtc ttgacgcttg ccgcgtagcg cgtggcgagc ggattggcga
     4021 gcccgaaggt tgtagcgcgc gccgcagcgt cggcgaggat gtcgccgacg atgtaggcgg
     4081 cgcgctcgtc gaagccctca aagcgcgcca gccgcctgta gccccgggtg agctcgagca
     4141 gagtgacttc cgctccgcca aggccgagcg cgtagccgta gtgatcagca tcccgctcca
     4201 gcgaaaagcc gacctcacgc agacggcggt ggaagggctc gtacccgacg agcgcaaggg
     4261 ttcgtaccgc gggcacgttg agcgagccgg cgagcgccgt gcgcagcgac actttgccgc
     4321 ggaagctgcg gtcgtagttc tgcggcacgt acagcccact gggcgtggta atggccgtcg
     4381 gcgtgtcgtc gaggagcgat gcggcggtga gtagccgccg ctcgatcgct agcgcgtaaa
     4441 ggaagggttt gagggtcgaa ccgggcaggc gctttgccgt cacgccgtcc acctgcgccg
     4501 cggaggacag ctcgccgctc gagccaaccc aggcgaggat atctccgcta gcgttgtcaa
     4561 gcaccagcgc cgcgccgtcc tcgacgttgc ggccgtcgag ctcgcgcaag tggcgctcca
     4621 gcgtctcggc ggcgaagcgc tgcaggcgcg cgtcgagagt cgagcgcctg cgctcgcccg
     4681 gggtcgtgag cagccggcgc gcgagatgcg gcgcatcgcc ttcaaggcgc ctgctgaccc
     4741 ccgccgcttt caagccggtc agggcgagcc attgaatctc ccggcatgcg gcgtcgtctg
     4801 ggcgcacgag cgcacaggcg cgacgcgtca cccgcgcgct agctgcattc ggcgcgcgga
     4861 ccagtgcggc gagcaacgcg ctctcggcgc gatcgagtcc gtcgggacgc ttggcgaaga
     4921 gcgagcgcga ggcagcatcg atgccctcga gctcgccgcg aaacggcgtg aggttgagcc
     4981 aggcctccag cagttcgtcc ttgttccagc ggcgctccat cgccatggct tgccgcatct
     5041 ggcgccattt gtgcacgagg ccgcggcttc cggagcgcgc cagctccgga tgcagccagg
     5101 cggcgagctg catggtgatg gtgctgccgc cgcgctggtc gcgcaaagca cccggcagcg
     5161 cgagccaatc gacgccggca tggccgagga aacgcctgtc ctccgccgcc aggaccgcga
     5221 cgatgagcgc aggagaaaca tcgcggagcg ccgtccagtc gccgcggcgg ctcgtcttgt
     5281 cgatgcgaat gcgcgacagc ggctcgccgt tgcggtctag cagccaggcg tcggaggagc
     5341 gccactcgct cttcacctcc gaatacggaa cgacgtcgta cggctctacg aacagggagg
     5401 cgacgaatcc agccgcaaaa aggaaacctg ccgaggtgcc caggagtttc ttcatggtct
     5461 cacctgcacg ccggcgtttg gcaactcgcc aaacatctcc ggcaggtaca ttgcctccac
     5521 gcgcgtcgcc ggcagatcga accggcccgg gttgttgagc cgcaccgtgt actcgatctt
     5581 gaaccgcccc ttcggcacga attcgtagta agcacggtag gcgtcgtgac tgcgctcgat
     5641 gaagaccggc caggcccagc cggcgcgctt ctcgcccgca gtgaacgaag ccgcgtcgcg
     5701 cccgagaccc gagccgagga tcgcggcgcc ggcgggaatg gggtcgtcga tcaccaccca
     5761 ggtcatgtcg gtctgcgcgt cgacctcgag cgtgacgcga tagacgtcgc cccgggaata
     5821 aatgctcttc tctttctgct cgactggcgt gatcgtgcgg cgaatggcgt aaccgctgga
     5881 tagaggcgct ttcagcggaa tcgcggcacg cgattgcacg atggcccacg gcgcgccctt
     5941 gcccgtgtgc gtgaggttaa gcatctcacg gcccgccggc caggcgatat cacgcagctg
     6001 cttttcgtgg ctgacggaca cggagactgc gctctgcccc agctgcgctt ccacgctacc
     6061 ggcggccggt gtcttctcga accgcccgct gaactgcccc gttgccacca cgccccacgc
     6121 gttcgcgacg gtggtgcccc agatacctcg cttctgccgg aagagcgcgc cgcgcaccat
     6181 ccggccgatg tcctcgcgcc agtcctccac gtcgagaacg gtgagcagcg cgcggtttgc
     6241 attgacgtcg gccgagacca tcagccacca cagcgcgtcc gttcgctcgg tggaaaaggt
     6301 catcgccgtg ccctggaagt tcagcctggc gcgcagctgc gacaacgcct cgtgcagccg
     6361 cagctcgccg tcgggcacgc cggctacgcg ttgcaggatt ccgatccagt cgatcagcgc
     6421 ggacgtgggc cagagcggcg ggtcgatcgt gatcgactcg agcatttgtg gcgtggcgcg
     6481 accgtggcgc gcgagcgcct cgatcgcggc gagcttgcgc ac
//
LOCUS       DASPXW010000365         2146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1117335, whole genome shotgun sequence.
ACCESSION   DASPXW010000365 DASPXW010000000
VERSION     DASPXW010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117335"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1500)
                     /gene="dxs"
                     /locus_tag="VEL04_08450"
     CDS             complement(<1..1500)
                     /gene="dxs"
                     /locus_tag="VEL04_08450"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009771556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HYT48437.1"
                     /translation="MYELLKRIDDPAQLRALERKQLGQLADELRAYVLESVSKTGGHL
                     SSNLGTVELTIALHYVFDTPEDRIVWDVGHQTYPHKILTGRREAMGRLRMIDGISGFP
                     RRAESKYDTFGTAHSSTSISAALGMAVAAKTKNEKRHCIAVIGDGAMSAGMAFEAMNN
                     AGAMEVDLLVILNDNEMSISPPVGALTNYLARLLSGRIYATARRASEHLLKRVGVWEL
                     ARRAEEHVKGMVTPSTLFEEFGFNYIGPIDGHDLDALIPTLTNIRKLKGPQFLHVITR
                     KGQGYKLAEDDPIAYHGPGKFNPAEGLQKASGGKPTYSQVFGEWLCDMAARDPRLVAV
                     TPAMREGSGMVRYSERFPERYFDVGIAEQHAVTFCAGLACEGMKPVLAIYSTFLQRGY
                     DQLIHDVAIQNLPVLFALDRGGIVGGDGQTHNGAFDYAYLRTLPNLTVMAPSDENECR
                     QMLYTGFRLDSPSAVRYPRGSGPGVAIEPEMRALPIGKGELRREGRRIAI"
     gene            complement(1551..>2146)
                     /locus_tag="VEL04_08455"
     CDS             complement(1551..>2146)
                     /locus_tag="VEL04_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HYT48438.1"
                     /translation="PTVHVEYDEATALLAGDALQSMAFQLLAEHRLADDARAQLEMVR
                     TLAAAAGSRGMAGGQSIDLDATGKTLTLAELEFMHIHKTGALIRAAVLLGVACGTELL
                     AEEKNKLDKYAKAIGLAFQVVDDVLDYDASTATLGKTAGKDSKQGKPTYVSALGIARA
                     KALAQELRSQAHAALADFGPRARRLAELADFIVLRKF"
BASE COUNT          323 a          720 c          721 g          382 t
ORIGIN      
        1 gattgcgatc cttctgccct cgcgccgcag ctcgcccttg ccgatcggca gagcgcgcat
       61 ctccggctcg atcgccacgc cggggccgct gccgcgcgga tagcgcaccg ctgagggcga
      121 atcgagcctg aagccggtgt agagcatctg gcggcattcg ttctcgtccg acggcgccat
      181 caccgtaagg ttcggcagcg tgcgcaggta ggcgtagtcg aatgccccgt tgtgcgtctg
      241 cccgtcgccg ccgacgatcc cgccgcggtc gagcgcgaac agcaccggca ggttctggat
      301 cgcgacgtca tggatcagct ggtcgtagcc gcgctgcagg aaagtcgagt agatcgcgag
      361 caccggcttc atcccctcgc aggcgagccc ggcgcagaag gtgacggcgt gctgctcggc
      421 gatgccgacg tcgaagtagc gctcgggaaa gcgctccgaa taccgcacca tgcccgagcc
      481 ctcgcgcatc gccggcgtga ccgcgaccag ccgcgggtcg cgtgccgcca tgtcgcacag
      541 ccactcgccg aacacctgcg aataggtcgg cttgccgcct gaagcctttt gcaggccctc
      601 ggccgggttg aacttgcccg gaccgtggta ggcgatcggg tcgtcctcgg cgagcttgta
      661 gccctgcccc ttgcgggtga tgacgtgcag gaactgcggc cccttcagct tcctgatgtt
      721 ggtgagagtg gggatgagcg cgtcgaggtc gtggccgtcg atcgggccga tgtagttgaa
      781 gccgaactcc tcgaacagcg tcgagggcgt caccatgccc ttcacgtgct cctccgcgcg
      841 ccgcgccagc tcccacacgc cgacgcgctt cagcaggtgc tcgctcgcgc ggcgggcggt
      901 cgcgtagatg cgtcccgaaa gcagccgcgc gagatagttg gtaagcgccc cgactggcgg
      961 cgagatcgac atctcgttgt cgttcaggat caccagcagg tcgacctcca tggcgccggc
     1021 gttgttcatc gcctcgaagg ccatgcccgc cgacatcgcg ccgtcgccga tcacggcgat
     1081 gcagtgccgt ttttcatttt ttgtttttgc cgcaacggcc atgccgagcg ccgccgagat
     1141 ggaggtggac gaatgcgccg tgccgaaggt gtcgtacttc gactcggcgc ggcgcggaaa
     1201 gccggaaatg ccgtcgatca tgcgcagccg gcccatcgcc tcgcggcgcc ccgtgaggat
     1261 cttgtgcgga taggtctggt ggccgacgtc ccagacgatg cggtcttcgg gagtgtcgaa
     1321 cacgtaatgc agcgcgatcg tcagctcgac cgtgccgaga ttggatgaca gatggccgcc
     1381 ggtcttcgac accgactcga gcacgtacgc ccgcagctcg tcggcgagct ggccgagctg
     1441 cttgcgctcg agcgcgcgca gctgcgccgg atcgtcgatg cgcttcagca gctcgtacat
     1501 cgacggattg tagtcgccgg ccggcagctc gccggtgatc gcgctcatgc tcagaatttt
     1561 ctcaggacga tgaagtcggc gagctcggcg aggcggcgcg cccgggggcc gaaatccgcc
     1621 agcgcggcat gcgcctgcga gcgcagctcc tgcgccagcg ccttggcgcg cgcgatgccg
     1681 agcgccgaaa cgtaggtcgg cttgccctgc ttcgaatcct tgcctgcggt cttgccgagc
     1741 gtcgccgtgc tggcgtcgta atcgagcacg tcgtccacca cctggaaggc gagcccgatg
     1801 gctttggcgt atttgtccag cttgtttttt tcttcggcga gaagctctgt tccgcaagca
     1861 acgccgagca gcaccgcggc gcggatcagt gcgcccgtct tgtggatgtg catgaattcg
     1921 agctcggcaa gcgtgagcgt cttgccggtc gcatcgaggt cgatgctctg gccgcccgcc
     1981 atgccgcgcg atcccgccgc cgctgccagc gtgcgcacca tctcgagctg cgcgcgcgcg
     2041 tcgtcggcaa gacggtgctc ggcaagcagc tggaacgcca tcgactgcag cgcatcgccc
     2101 gcgagcagcg cggtggcctc gtcgtactcc acgtgcacgg tcggct
//
LOCUS       DASPXW010000366         5582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1372128, whole genome shotgun sequence.
ACCESSION   DASPXW010000366 DASPXW010000000
VERSION     DASPXW010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5582
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1372128"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="VEL04_08460"
     CDS             <1..709
                     /locus_tag="VEL04_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48439.1"
                     /translation="EAKREREEKVGAMKKQAEANIAQARAQQKGDTDRQLKECATGAD
                     SMEFARLGIYGRCYRKGDKDCKGMVDGLSQVSPELVKGCTAKAAEYCKRFQTQEGFLK
                     AKGDEAAAKGCGVSTASIKAAQCPRAAQSGSLAFLGAFCPVEAKPIAQEHCAGRDYTS
                     KTGGKYAVFCQSYLANADFEKDESQPAARQGASPSRSQPATPPSGSPPETPPSGSDQV
                     KQGINKGLDKLKGLFGR"
     gene            720..1514
                     /locus_tag="VEL04_08465"
     CDS             720..1514
                     /locus_tag="VEL04_08465"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HYT48440.1"
                     /translation="MRLGLVLVLAASLVAGAARAADDPYPWIAAAYVVKRDGALLWSG
                     SADRRLAPASLAKLMTALLALERGGLDEAVEVGRGVLQASGTRMGLKPGEKLRAGDLL
                     AATVVRSANDACRALAEHLGKTVANFVLGMNQRAAALGLANTHFADPCGHDREGQYAS
                     AADLMVLAEQLMRHDEYLRLARLERLTVRTLDRSRSFALRNTNALIGRYPGAIGLKTG
                     YSEGAGNCLVALAERDGVRVLAVLLNAPNRWWNAVGLLDRAFAAPR"
     gene            1511..2785
                     /locus_tag="VEL04_08470"
     CDS             1511..2785
                     /locus_tag="VEL04_08470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HYT48441.1"
                     /translation="MRPALAVAALAAAAYLPSLAGVFHFDDYNVIVNYLTVHSWQALF
                     ERAGDGVRPLLKASYTLNWTLGFGFSFLNILIHAVNAVLLYLVGKDLFKNQSAALVAA
                     LLFALHPAQTEAVTYISGRSSSLMALFYLGALLAYLRGAHWSVSSLLFLFAMATRETA
                     VTLPAALLLCELCRTERPGWKEILRRQGPHWALLLAGGLFLVANQRYFDLVAHGYTER
                     SLADNLLTQVGGVSYLVLRLVSLHGYNIDPALPTLTQWTPALGFQAVLFVMLFILGIA
                     NLRSRPWIAFGVLWFFLQLAPTNSVVPRLDVANERQLYLACWGLFLALSVQFSKINLS
                     TRKIVASAGVVLTVFLATSIFRQLEYRSEIALWESSVALAPWNARAHNNLGYAYQLAG
                     RGQDARREYETALVFEPRHAKARLNLMFLPPE"
     gene            complement(3677..>5582)
                     /locus_tag="VEL04_08475"
     CDS             complement(3677..>5582)
                     /locus_tag="VEL04_08475"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT48442.1"
                     /translation="SRFLRSSAPALVELPGGTGPHFLPICGRRGRDILVVGQDLSVYP
                     LRTETLCSMLRQPLVENLATGVERLLEHASVPAGRRLKVRAALLEGQLRSLPIGGCWL
                     LRASDAEALSARARRDRLLAPVALVAGAHAAQHILLVLSWWVLGTGLLQGRSDAGWLL
                     AWALLLLSMIPFRALEQWSLGWVAARLGVVMRQQLLERILHLQLDVVRRQGAGQLLGR
                     VMDAEALESLVLSGGHVGLVAGVELLIAVPVLLAGAGGALHAALLGSWVIVSLLMSRR
                     YWRLRREWAAARLAITEQLVESMVGYRTRLAQEEPARWHGGEDRALADYAALAKPMDR
                     SAVWLKALLPRGWLIAGLAGLAPALVSGQATVGSVAVAVGGVLLAYRALWKAVRGLSD
                     LVDANIAWSRVAPILHGTDRAASDVSRAPLGGAPLPSGESVLVQARGLAFRYPSRSMP
                     VLQGCDLRIRGGDRIVLEGTSGAGKSTLAALLAALRVPDSGLLLLGGLDRYTLGSTAW
                     RRRVVTAPQFHENHVLTGTFAYNVLMGRRWPPEPEDLAAAEAVCCKLGLGDLLKKMPA
                     GLMQMIGDTGWQLSHGERSRLFMARALLQEPDLLIVDERLGALDSETQAQCLDVLLEE
                     ARALILISHL"
BASE COUNT          968 a         1910 c         1782 g          922 t
ORIGIN      
        1 ggaggcgaag cgcgagcgcg aggagaaagt cggtgcgatg aagaagcagg ccgaggccaa
       61 catcgcgcag gcgagggccc agcagaaggg ggacaccgac cggcagctca aggaatgcgc
      121 gaccggtgcc gactccatgg aattcgccag gctcggaatc tatggccggt gctacaggaa
      181 gggcgacaag gattgcaagg ggatggtgga cgggcttagc caggtgagcc cggagctggt
      241 aaaaggctgc accgccaagg cggccgaata ctgcaagcgc ttccagacgc aggaaggctt
      301 tctgaaggcg aagggcgacg aggccgcggc gaagggatgc ggcgtgagca ccgcctcgat
      361 caaggcggcg caatgcccgc gcgcggcgca gagcggatcg ctggctttcc tgggcgcgtt
      421 ctgcccggtg gaggcgaagc cgatcgccca ggagcactgc gcggggcgcg actacacctc
      481 gaagacgggc ggcaagtatg ccgtgttctg ccagagctac ctcgcgaacg ccgatttcga
      541 aaaagacgaa tcacagcccg cggcgcggca gggggcttcc ccgtccaggt cgcagcccgc
      601 aacgcccccg tccgggtcgc cgcccgagac tcccccgtcg gggtccgatc aggtgaagca
      661 gggcatcaat aagggtctgg acaagctgaa gggcctcttc ggccgctagc ggcggagaga
      721 tgcggctggg gctcgtcctg gtccttgcgg cgtcgctcgt cgcgggcgca gcgcgagctg
      781 ccgacgatcc ctacccctgg attgccgctg cctacgtggt gaagcgcgac ggcgcgctgc
      841 tatggtcagg atcggctgac cggcgtttag cgccggcgag cctcgccaag ctcatgaccg
      901 cgctcctcgc gctcgagcgc ggcggcctgg acgaggcggt cgaggtcggc cgcggcgtgc
      961 tgcaagcgag cggcacgcgc atgggcctca agccggggga gaagctgcgc gcgggcgacc
     1021 tgcttgccgc aaccgtggtg cgctcggcga acgacgcctg ccgcgcgctc gccgagcatc
     1081 tgggcaagac tgtagctaat ttcgttctgg gaatgaatca gcgcgccgcg gcgctcggcc
     1141 tcgccaacac gcacttcgcc gatccctgcg gccacgaccg cgaaggccag tacgccagcg
     1201 ccgccgacct gatggtcctc gccgagcagc tgatgcgcca cgacgaatac ctgcgcctcg
     1261 cgcgcctcga gcgcctcacg gtacgcacgc tcgaccggag ccgcagcttt gcgctgcgca
     1321 acaccaacgc gctgatcggg cgctatcccg gcgccatcgg cctgaagacc ggctacagcg
     1381 agggcgccgg caactgcctg gtggcgctcg cagagcgcga cggcgtgcgc gtgctggcgg
     1441 tgctgctgaa cgcgcccaac cgctggtgga acgcggtggg gctgctcgac cgggcgttcg
     1501 ccgcgccgcg atgaggccgg cgctcgccgt cgcggcgctc gcggcggcgg cctacctgcc
     1561 gtcgctcgcc ggcgtgttcc atttcgacga ctacaacgtc atcgtcaact acctgacggt
     1621 gcattcctgg caggcgcttt tcgagcgcgc cggggacggc gtgcggccgc tgctgaaagc
     1681 gagctacacg ctgaactgga ctcttggctt tggtttcagt tttttgaata tcttgattca
     1741 tgccgtgaac gccgtattgc tgtacctcgt cgggaaagat ctgttcaaga atcaaagtgc
     1801 agctttggtt gccgcgctgc tcttcgccct ccatcctgcg caaaccgagg ccgtcaccta
     1861 catcagcggc cgttcctcgt cgctgatggc gctcttctac cttggagcgc tgctcgcgta
     1921 cctgcgcggc gcgcactggt cggtctcttc gcttctcttc ctcttcgcca tggcgactcg
     1981 ggagaccgcg gtcacgctgc ccgccgcgct gctgctttgc gagctgtgcc gcacggagcg
     2041 gcccggctgg aaggagatcc tgcgacgtca ggggccgcac tgggcactgc tcctcgcggg
     2101 cggcctcttc ctggtcgcga accagcgcta tttcgacctc gtcgcccacg gctataccga
     2161 gcgcagcctg gcggacaatc tcctcaccca ggtcggcggc gtgtcctatc tcgtgctgcg
     2221 cctcgtctcg ctgcacgggt acaacatcga tcccgcgctc ccgacgctca cgcaatggac
     2281 gccggcgctc ggattccaag ccgttctctt tgtaatgctg ttcatcctgg gcatcgccaa
     2341 cctgcgctcg cggccgtgga tcgccttcgg cgtgctgtgg ttcttcctgc agctcgcgcc
     2401 caccaactcg gtcgtgccgc gcctggacgt ggcaaacgag cggcagctgt acctcgcgtg
     2461 ctggggcctg tttctcgctc tttctgttca gttctccaaa ataaatctat cgacaagaaa
     2521 aatcgtcgca agcgccggcg ttgtgctgac tgtgttcctg gcgaccagta ttttccgtca
     2581 gctcgagtat cgcagcgaga tcgcgctgtg ggagtcgagc gtcgccctcg cgccgtggaa
     2641 cgcgcgtgcg cacaacaacc tgggctacgc ctaccagctc gccgggcgcg gccaagatgc
     2701 gcggcgggaa tatgaaacgg cgctcgtctt cgagccgcgc catgcgaagg cgcggctgaa
     2761 cctgatgttc ctgccgccgg agtagacttt cccgcgcatg gcgaccgccg caggcaggac
     2821 cttcctggag cgcctcgatg acgcggcgcg caagctcctg cattcccccc gagcgtcgaa
     2881 gatgcccacc ggagttacca aaaataaagg caggtttgca cctgccttta ttttttgaca
     2941 acgcgactct gttacgacga accaacccta ccacgacagc gcgtagttag tcccacgcga
     3001 cactcccggc cctcacattc gtggttacct tcatcggtta tcacctcctt ggtgtgcggt
     3061 gcttcgacag ttgttggagc gatgctacaa aggcactgac ctaccacgcg atcgcgtagt
     3121 cactcccggc cttcacattc gtggtcccgc gatgatcggc gactaatcta gaagtcgccg
     3181 ccgcctccgt cgtcgcggta cgaaccggcc ttcaatcccg acttgatctt catggacctc
     3241 tcctttctgc gaaggacaaa ttgatcgttc gcaggttatt tgcaccgctc accgacccga
     3301 aacacgccgc aaccgcccgc atttcgcggc acggacgcga aggaatttcc tatccttctc
     3361 tgttacggcg aaccgaccct accacgcgat cgcgtagtca ctcccggcct tcacattcgt
     3421 ggtcaccttc atcgcttatg acctcctgcg gcgctacgac agttgttgga gctatgctac
     3481 aaagggactg gcctaggttc agtcgagtac ggcgacgctg gcctgaccgg ccttcaggtt
     3541 ggtcttgacc ttcacgtttc tcacctccct ccctggcgtt gcggcgctac gacagttgtt
     3601 ggagcgatgc tgcacttagc gatgcgacgc tacgccccaa gcggggaaac tgtcaagctg
     3661 ggacgccgcg ccctctttac aggtgggaga tgaggatgag ggctcgcgcc tcctcgagga
     3721 ggacgtccaa gcactgcgcc tgtgtctccg aatcaagcgc accgagcctt tcatcaacaa
     3781 tcaggagatc gggctcctgg agcagcgccc gcgccatgaa caggcggctg cgttcgccgt
     3841 gggagagttg ccatcccgtg tcaccgatca tctgcatgag gccggcgggc atcttcttca
     3901 gcaagtcgcc gagaccgagc ttgcagcaga ccgcctcggc agccgcgaga tcctcgggct
     3961 ccggaggcca gcgccgcccc atgagaacgt tataggcgaa cgtccccgtg agaacgtggt
     4021 tctcgtgaaa ctgcggggcg gtcacgacgc gtcggcgcca ggctgtcgac cccagcgtat
     4081 agcggtccag tccgccgagc agcaggagtc cggagtcggg cacgcgaagc gcggcgagca
     4141 gcgccgcgag cgtcgactta ccggccccgg atgttccttc gagcacaatc ctgtcgccgc
     4201 cccggattcg gagatcacaa ccttgaagca caggcatgga ccgactcgga taccggaacg
     4261 cgaggccgcg cgcttgcacc agcacgctct cgccggacgg gaggggcgcg ccgccgagag
     4321 gtgccctcga gacgtcgctc gcggcccggt ccgtcccgtg cagaatgggc gcgactcggc
     4381 tccaggcgat gttcgcgtcc accaggtcgg agaggccccg caccgccttc caaagcgcgc
     4441 ggtaggcgag caacacgccc cccaccgcga ctgcgacgga accgactgtg gcttgcccgc
     4501 tcaccagggc cggagcaagg ccggcaaggc ccgcgatgag ccaccctcga ggtagcagcg
     4561 ccttcagcca cacggcgctt cgatccatgg gcttggccag ggctgcgtag tccgccaacg
     4621 ctcggtcctc accgccgtgc caacgcgcag gctcctcttg ggccagccgc gtgcggtacc
     4681 ccaccatgga ttccaccagt tgttccgtga tggcgagccg cgcggcggcc cactctcgac
     4741 ggagccgcca gtaccgtctc gacatgagca gcgatacgat gacccacgac ccaagcagcg
     4801 ccgcgtggag cgcgcctccg gcgccggcga gcagtaccgg caccgcgatg agcagctcga
     4861 cgcccgcgac caacccgacg tggccaccgc tcaacacgag cgactcaaga gcttcggcgt
     4921 ccatcacgcg accgagcaac tggcctgcgc cctgtcgccg gaccacgtcg agttgcagat
     4981 gaaggatccg ttccagcaac tgctgcctca tcaccacccc gagcctggcc gcgacccagc
     5041 ccaacgacca ctgctccagc gcccggaacg ggatcatgga cagcaggagt agggcccatg
     5101 caagcagcca ccccgcgtcc gaacgcccct gcaagagccc ggtgcccaat acccaccatg
     5161 agagcaccaa gaggatgtgc tgggcggcat gagcccccgc tacgagggcc acgggagcga
     5221 gcagccggtc acggcgcgcg cgcgcggaga gcgcctcggc gtcggaagcg cggagcagcc
     5281 agcagccgcc gatgggaagc gaacggagct gtccttccag cagcgccgcg cggaccttga
     5341 gccgccggcc ggcgggcacg ctggcgtgct ccagcaaccg ttccacgccg gttgccagat
     5401 tctcgacgag gggttgccgc agcattgagc acagggtctc ggtccgaagc ggatagacgg
     5461 acaagtcttg tcccacgacg agaatgtcac gcccgcgacg gccacatatc ggcaggaaat
     5521 gcggcccggt tcctcccggt agctccacca gagccggtgc gctggagcgg agaaaccggc
     5581 tc
//
LOCUS       DASPXW010000367         7339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1117961, whole genome shotgun sequence.
ACCESSION   DASPXW010000367 DASPXW010000000
VERSION     DASPXW010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7339
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117961"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="VEL04_08480"
     CDS             complement(<1..858)
                     /locus_tag="VEL04_08480"
                     /inference="COORDINATES: protein motif:HMM:NF015825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HYT48443.1"
                     /translation="MRVFAILGVSLGLAAGAVQAQSPETLGWLRKMQQATQKLSYTGT
                     FVYQQGARSETSRITRYVDGRAGDIERLEVLDGVPREIVRTRDTVKCYLPDSRVVKID
                     RRTPDRSFPALLPEKSAALARYYDVSLGETRRIAGFDCQAVVLTPKDNLRYGYRLYAD
                     VASGMLLKAVTFDAAGESVEQFMFTQLAIGNVTRDMVKPRHAARGWRVEDAAASPAHL
                     TGWGLSAELPGFHKIIELKRRMGESKPVGQVVYSDGLAAVSVFIEPLEARREPSRTGL
                     ASLGAIHIYT"
     gene            complement(855..1316)
                     /locus_tag="VEL04_08485"
     CDS             complement(855..1316)
                     /locus_tag="VEL04_08485"
                     /inference="COORDINATES: protein motif:HMM:NF015810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HYT48444.1"
                     /translation="MQDRISALMDGELDDRDAGDAIDTLGREHAALEAWRTYHLIGDA
                     MRMSHRLTAEPGLAPVRLAARPRLRFALPLAASAAAVALVGWVAFAPQQAAPPALAPV
                     VAEAKPPVMVPLSAAANDYLLAHQGFSPRVSFQGMAPYVRTVSDQGQEKRR"
     gene            complement(1342..1941)
                     /gene="rpoE"
                     /locus_tag="VEL04_08490"
     CDS             complement(1342..1941)
                     /gene="rpoE"
                     /locus_tag="VEL04_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HYT48445.1"
                     /translation="MSDREIDRQLVARAQRGDKQAFELLVEKYQRKLARLLSRFIRDP
                     AEVEDVTQEAFIKAYRALPAFRGDSAFYTWLYRIGINTAKNYLMAMGRRAPTSTEVEA
                     EEAEGFEEGEQLRDINTPESLLLSNEIAETVNSTIEKLPEELRKAIQMREIEGMSYED
                     IAQAMDCPIGTVRSRIFRAREAIAEQLRPLLGTSKDKRW"
     gene            complement(2055..2504)
                     /locus_tag="VEL04_08495"
     CDS             complement(2055..2504)
                     /locus_tag="VEL04_08495"
                     /inference="COORDINATES: protein motif:HMM:NF018908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HYT48446.1"
                     /translation="MSARAPLRLELAPSRALAATIVALHATAAVCVLVVLPSTAGAVL
                     AGCLMFLGLAAAWGRALLKSSASVRALEVSGSEVTLELASGERIVCRPAGRRYVNRFM
                     VSLPLRPPMRRTILITGDMLERDAFRRLRIWALWGKLPGVETRQPPA"
     gene            complement(2501..3739)
                     /gene="fabF"
                     /locus_tag="VEL04_08500"
     CDS             complement(2501..3739)
                     /gene="fabF"
                     /locus_tag="VEL04_08500"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006682911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HYT48447.1"
                     /translation="MTRRRVVLTGLGIVCPIGNTVQQAWDSALAGRPGITRITRFDAS
                     RLASQIAGEVKGFDVSSYMLPKEARRMDQFIHYGMAAGLQAWRDCGLQAGAQNAERIG
                     INFGSGIGGLPLIETMHDELKKSGPRRISPFFIPGSIINMIAGLLSIQIGAKGPNLAM
                     VTACTTSTHCIGEAAKSIRYGEVEVMIAGGAEAVVTELAVGGFASARALSTRNDDPAT
                     ASRPWDKERDGFVLGEGAGAVVLEEYEHAKARGANIYCELVGYGVSADAFHMTAPPED
                     GDGGFRAMRNALKDANLESSAIDYINAHGTSTPLGDLAETKAVKRLLGGHAAKVAVNS
                     TKSMTGHLLGAAGGVEAVFTALAIRDQVSPPTINLRTPDPECDLDYVPNAARRMPIRV
                     ALSNGFGFGGTNGTLVMARL"
     gene            complement(3740..3979)
                     /gene="acpP"
                     /locus_tag="VEL04_08505"
     CDS             complement(3740..3979)
                     /gene="acpP"
                     /locus_tag="VEL04_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HYT48448.1"
                     /translation="MENIEQRVKKIIAEQLGVNEAEIKTESSFVDDLGADSLDTVELV
                     MALEEEFETEIPDEAAEKITTVQQAIDYVKAHAKS"
     gene            complement(4034..4753)
                     /gene="fabG"
                     /locus_tag="VEL04_08510"
     CDS             complement(4034..4753)
                     /gene="fabG"
                     /locus_tag="VEL04_08510"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HYT48449.1"
                     /translation="MSLLEGRLALVTGASRGIGRAIAIELARQGAKVVGTSTSAAGAM
                     GISGIGKVLNVRDAAQCDALVESIQKEHGAVLILVNNAGIARDNLVLRMKDADWDEVI
                     ETNLRAVFRLSRAVMRGMMKARWGRIINISSVVGAAGNPGQANYAAAKAGVVGMTKSL
                     AAELGSRNVTVNCVAPGFIDTDMTRALTEAQRTALLERIPLGRLGAPEDVAAAVAYLA
                     SPAAGYVTGAVLHVNGGMYMS"
     gene            complement(4750..5679)
                     /gene="fabD"
                     /locus_tag="VEL04_08515"
     CDS             complement(4750..5679)
                     /gene="fabD"
                     /locus_tag="VEL04_08515"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HYT48450.1"
                     /translation="MKLALVFPGQGSQVVGMLKGYAGLPGIEETLEEARQALGAPFVR
                     LLDEGPTEMLNQTINTQPAMLTAGYAAYRAWRALGGPQPELVAGHSLGEYTALVAAET
                     IRFLDCLPLVRFRAQAMQEAVPQDEGAMAAILGLDDNGVRQACAEAGGAVQAVNFNSP
                     GQVVIAGEKRAVERASELCKSRGAKRALPLPVSAPFHSSLMRPAGEKLRVYLEKVALA
                     APKIKLVNNVDVKIENHPLAIKDALVRQAYSAVRWVETIAKMRELGVTRVIECGPGRV
                     LAGMTKRIDAGMQSAALADRASLQKALQAVGAA"
     gene            complement(5676..6635)
                     /locus_tag="VEL04_08520"
     CDS             complement(5676..6635)
                     /locus_tag="VEL04_08520"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008162005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HYT48451.1"
                     /translation="MIYSRIAGTGSFLPPRVMTNQEFAARLETSDEWIRSRTGIERRH
                     IAEASQASSDLALEASRKALESAQIGPHRLDLIIVATSTPDYVFPSTACLLQAKLAIK
                     GCAAFDVQAVCSGFVYGLATADSFIKSGMSKCALVVGAEVFSRILDWNDRGTCVLFGD
                     GAGAAVLVPSATPGVHASILRADGSQAGLLSVPGNLSGGCVIGSPFVQMNGQAVFKFA
                     VKVLEESARETVAAAGMTIEDIDWLIPHQANVRILEATARKLGLPLEKLVVTVDHHGN
                     TSAASVPLALDEFIRAGKIRVGQRVLLQGVGGGFTWGSSLVTV"
     gene            complement(6632..>7339)
                     /gene="plsX"
                     /locus_tag="VEL04_08525"
     CDS             complement(6632..>7339)
                     /gene="plsX"
                     /locus_tag="VEL04_08525"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009913176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HYT48452.1"
                     /translation="GNTGALMAISRFVLKTLPGIDRPAIATVLPNMRRTNTYMLDLGA
                     NVDCTAENLLQFGMMGAMLVAAIEHKERPLVGLLNIGVEDIKGNETVKQAAELLRASG
                     LNFAGNVEGDDIYRGNIDVVVCDGFVGNVALKASEGVVQMMTSTLREEFKRNPFTRLA
                     ALVSLPVLRAFRGRMDHRRYNGASLLGLRGIVIKSHGSADVFAFGQALSRAVEEVRNE
                     VPQRIERRMAERQAVAR"
BASE COUNT         1174 a         2540 c         2373 g         1252 t
ORIGIN      
        1 cgtatagata tggatcgcgc cgaggcttgc aagtcctgtc cgggacggct cgcgccgggc
       61 ttccagcggc tcgatgaaca ccgacaccgc ggcaaggccg tcggaataga cgacctggcc
      121 gaccggcttc gactcgccca tgcggcgctt gagctcgatg atcttgtgga atccgggcag
      181 ctcggcggag agtccccagc cggtgagatg cgccggagat gccgcggcgt cttccaccct
      241 ccagccgcgc gccgcgtggc gcggcttcac catgtcgcgg gtgacattgc cgatcgcaag
      301 ctgcgtgaac atgaactgct cgacgctctc cccggcggcg tcgaaagtga ccgccttgag
      361 cagcatgccg ctcgccacgt cggcgtacag ccggtagccg tagcgcaggt tgtccttggg
      421 cgtgagcacc accgcctggc aatcgaagcc ggcgatgcgt cgcgtctcgc ccaggctgac
      481 gtcgtagtag cgcgcgagcg ccgcgctctt ctcgggcagc agcgccggga agctgcgatc
      541 gggcgtgcgc cggtcgattt tcaccacgcg gctgtcgggc aggtagcact tcaccgtatc
      601 gcgcgtgcgc acgatctcgc gcggcacgcc atcgagcacc tcgagccgct cgatatcgcc
      661 cgcgcggccg tcgacgtagc gcgtgatgcg cgaagtctcg ctgcgcgcgc cctgctgata
      721 gacgaaggtg ccggtatagg aaagcttctg cgtcgcctgt tgcatcttgc gcagccagcc
      781 gagcgtttcg ggcgactgcg cctgcactgc gcctgcggcg agaccgaggg aaacccccag
      841 gatcgcgaaa accctcaccg gcgcttttcc tgcccctggt cggaaaccgt gcgcacgtac
      901 ggcgccatgc cctggaacga cacccggggc gagaagccct ggtgggcgag cagatagtca
      961 ttcgcggcgg cggagagcgg caccatgact ggcggcttcg cttccgccac cacgggcgcc
     1021 agcgccggag gagctgcttg ctggggcgca aacgcgaccc agcccaccag ggccaccgcg
     1081 gcagcgctgg ccgcgagcgg cagggcgaag cgaagccgcg gccgggccgc gaggcgcaca
     1141 ggagcgagcc ccggctcggc cgtgagccgg tggctcatgc gcatcgcatc gccgatcagg
     1201 tgataggtgc gccaggcttc gagcgcagcg tgctcgcgtc ccagcgtatc gatcgcatcg
     1261 ccggcgtccc tgtcgtcgag ctcgccgtcc atcaatgccg aaattcggtc ctgcatagcc
     1321 cctaccacct gccttctggc cttaccacct tttgtccttg ctcgtcccca gcaaaggccg
     1381 caactgttcc gcgatcgctt cccgggcgcg gaagatccgc gaccgcaccg tgccgatcgg
     1441 gcagtccatc gcttgcgcga tgtcctcgta actcatgcct tcgatctcgc gcatctgaat
     1501 cgccttgcgc aattcctccg gcagcttttc gatcgtggag ttcactgtct ccgcgatttc
     1561 attcgacagc aacaggctct cgggcgtgtt gatgtcgcgc agctgctcgc cttcctcgaa
     1621 tccttccgcc tcctcggcct ccacctcggt ggaggtcggc gcccgccgac ccatggccat
     1681 gaggtagttc ttggccgtgt tgatgccgat cctgtacagc cacgtgtaaa acgcgctgtc
     1741 gccacggaat gcggggagcg cccggtaggc cttgatgaac gcctcctgcg tcacatcttc
     1801 gacctcggcc ggatcgcgga tgaagcgaga cagcagccgc gccagcttgc gctggtactt
     1861 ctctacaagc agctcgaagg cctgcttgtc gccacgctgc gcccgcgcga ccagctgccg
     1921 gtcgatttcg cggtcagaca tcggaccgcg cccgcccgtc gccgcgcggc aagggctccc
     1981 caccccgttt gcaacgccta tgtgccacag acacacgcac ctggaaatag ttcgaagtag
     2041 tcgccggccg ccgctcaggc cggcggttgc cgggtctcca cgccgggcag cttgccccac
     2101 agcgcccaga tgcgcagccg ccggaaggcg tcgcgctcca gcatgtcgcc ggtgatcagg
     2161 atggtgcgcc gcatcggggg acgcagcggc aggctcacca tgaaccggtt gacgtagcgg
     2221 cgcccggccg gccggcagac gattctttcg ccgctcgcga gctcgagagt gacttcagat
     2281 cctgagactt ccagggcgcg caccgatgcc gaggacttca gcagcgcgcg gccccacgcg
     2341 gcggccaggc caagaaacat caggcaacct gccagcacgg cgcctgcagt cgagggcaat
     2401 accaccaaaa cgcaaacggc cgccgtggca tgcagcgcga cgatggttgc cgccaaggcg
     2461 cgggatggcg cgagctcgag ccgcagaggg gcccgggcgc tcaaaggcgc gccatgacca
     2521 gcgtgccgtt ggtgccgccg aagccgaatc cgttggagag cgcgacgcgg atcggcatcc
     2581 tgcgggccgc gttcggcacg taatcgaggt cgcactccgg gtccggcgtg cgcaggttga
     2641 tggtcggcgg cgaaacctgg tcacggatcg cgagcgcagt gaataccgcc tccacgccgc
     2701 ccgccgcgcc aagcaggtgg ccggtcatgg atttggtcga gttcactgcc acctttgccg
     2761 catgtccgcc gaggagccgc ttcaccgcct tggtctcggc gaggtccccg agcggggtcg
     2821 aggtaccgtg cgcgttgatg taatcgattg ccgagctctc gagattcgcg tccttgagag
     2881 cgttgcgcat ggcgcgaaag ccgccgtcgc cgtcctcggg cggagcggtc atgtgaaagg
     2941 cgtcggccga gacgccgtag ccgaccagct cgcagtagat gttggcgccg cgcgccttgg
     3001 catgctcgta ttcctcgagc accaccgcgc cggcgccctc gcccagcacg aagccgtcgc
     3061 gctccttgtc ccaggggcgg ctggcggtgg caggatcgtc gttgcgcgtc gagagggcgc
     3121 gcgctgaggc gaagccgccg acggccaact cggtgaccac ggcttcggcc ccgcccgcga
     3181 tcatgacctc gacctcgccg tagcggatcg acttggccgc ctcgccgata caatgcgtcg
     3241 aagtggtgca ggcggtgacc atcgccaggt tcggcccttt ggcgccgatc tggatcgaca
     3301 gcagcccggc gatcatgttg atgatgcttc ccgggatgaa gaacggcgag atgcggcgcg
     3361 ggccgctctt cttcagctcg tcgtgcatgg tttcgatcag cggcaggccg ccgatgcccg
     3421 agccgaaatt gatgccgatg cgctcggcat tctgcgcgcc cgcctgcagc ccgcagtcgc
     3481 gccacgcctg cagcccggcg gccatgccgt aatggatgaa ctgatccatg cggcgcgcct
     3541 ccttcggcaa catataagac gagacgtcga agccctttac ctcgccggcg atctggctcg
     3601 cgaggcgcga cgcgtcaaag cgcgtgatgc gggtgatgcc gggtcggccg gcgagcgcgc
     3661 tgtcccaggc ttgctggacc gtgttgccga tcggacagac gatgccgagc ccggtgagga
     3721 ccactctccg gcgggtcact taggacttgg cgtgagcctt gacgtagtcg atggcctgct
     3781 gcacggtggt gatcttctcc gccgcctcgt ccgggatctc ggtctcgaac tcctcctcca
     3841 gcgccatcac cagctcgacg gtatccagcg agtcggcgcc cagatcgtcg acgaacgacg
     3901 actcggtctt gatctcggcc tcgttcaccc cgagctgctc ggcgatgatc ttcttgacgc
     3961 gttgttcgat gttttccatc cgctgctcct tccagattga agggttgcgc tgcaggccaa
     4021 aagggtccgt attttacgac atatacatgc cgccgttgac gtgcagcacc gcgccggtga
     4081 cgtatccggc cgcaggcgac gcgagataag caaccgccgc ggcgacgtct tccggcgcgc
     4141 ccagccgtcc gagcggaata cgctcgagca aagccgtgcg ttgcgcttcg gtgagcgcgc
     4201 gcgtcatgtc ggtatcgatg aaacccggag cgacgcagtt caccgtgacg ttgcggctgc
     4261 cgagctcggc ggcgagcgac ttcgtcatgc cgaccacccc ggctttcgcc gccgcgtaat
     4321 tcgcctggcc aggattgccg gcggcgccga ccacggagct gatgttgatg atcctgcccc
     4381 agcgcgcctt catcatgccg cgcatcaccg cgcgcgagag cctgaacacc gcgcgcagat
     4441 tggtctcgat gacttcgtcc cagtccgcgt ccttcatgcg cagcacaagg ttgtcgcgcg
     4501 cgattccggc gttgttgacg agaatcagca cagcgccatg ctctttctgg atcgactcga
     4561 ccagcgcatc gcattgcgcc gcatcgcgca cgttcaggac tttcccgatc cccgaaatgc
     4621 ccattgcgcc ggctgcgctg gtcgaagtgc ccaccacctt cgcgccctgc ctcgcaagct
     4681 cgatcgcgat ggcgcggccg atgccacgcg aggctcccgt gaccagcgcg agcctgcctt
     4741 cgagcaggct catgctgcgc cgaccgcctg caaagctttc tgcagcgagg cgcgatcggc
     4801 gagcgcggcg gattgcatgc ccgcgtcgat tctcttcgtc atcccggcga gcacccttcc
     4861 gggcccgcac tcgatcacgc gggtcacccc cagctcgcgc atcttcgcga tggtttcgac
     4921 ccagcgcacc gctgaatacg cctggcgcac cagcgcgtct ttgatcgcga gcgggtggtt
     4981 ctcgattttc acgtcgacgt tgttcaccag tttgattttt ggagctgcaa gagcaacctt
     5041 ctcaagatag acgcgcaact tttctccggc aggtcgcatc aagctcgaat ggaacggcgc
     5101 gctcaccgga agcggcaagg cgcgttttgc gccgcgcgac ttgcacagct cggaggcgcg
     5161 ttccaccgcg cgtttttcgc cggcgatgac tacctgcccc ggcgaattga aattgaccgc
     5221 ctgcactgct ccgcccgcct cggcgcaggc ctggcgcacg ccattgtcat ccaggcccag
     5281 gatcgccgcc atcgccccct cgtcttgcgg cacggcctcc tgcatggcct gcgcgcgaaa
     5341 gcgcaccagc ggcaggcagt cgaggaagcg gatcgtctcg gccgcgacca gggccgtgta
     5401 ctcgcccagg ctgtgacccg cgacgagttc cggctgcggt ccgccgagcg cgcgccatgc
     5461 acggtaggcg gcgtatccgg cggtgagcat cgcgggctgc gtgttgatgg tctggttgag
     5521 catttcggtg gggccttcgt cgagcaagcg gacaaatggc gccccaaggg cctgtcgtgc
     5581 ctcttccagg gtttcctcta ttccgggaag gcctgcgtag cccttcagca tgccgaccac
     5641 ctgcgagccc tgacccggaa aaaccagcgc gagcttcata ccgtaacgag cgaggagccc
     5701 caggtgaagc cgccgccgac gccttgcagc agcacgcgct gcccgacgcg gatcttcccg
     5761 gcgcgaatga actcgtcgag cgccaggggc accgaggcgg ccgaagtatt gccatgatgg
     5821 tcgacggtga cgaccagctt ctccagcggc agcccgagct tgcgcgcggt cgcctcgagg
     5881 atgcgcacgt tcgcctggtg cgggatcagc cagtcgatgt cctcgatggt catgccggca
     5941 gcggcgacgg tttcgcgcgc cgactcctcg agcaccttga ccgcgaactt gaacaccgcc
     6001 tggccgttca tctgcacgaa aggcgagccg atgacgcagc cgcccgagag attgcccggc
     6061 acggagagca gccctgcctg gctgccatct gcacgcagga tgctcgcatg cacgcccggc
     6121 gtggccgagg ggaccagcac cgcggcgccg gcaccatcgc cgaacagcac gcaggtgccg
     6181 cgatcgttcc agtcgaggat gcgcgagaag acctcggcgc ctaccaccag cgcgcatttc
     6241 gacattccgc tcttgatgaa gctgtccgcg gtcgccaggc cgtagacgaa gccgctgcac
     6301 accgcctgca cgtcgaacgc cgcgcagccc ttgatcgcca gcttggcctg gagcaggcag
     6361 gcggtgctcg ggaacacata gtccggcgtc gaggttgcga caatgatcag gtcaagccga
     6421 tgcgggccga tttgcgcgct ttccagggct ttgcgactcg cttccagcgc caggtcgctg
     6481 gaagcctgcg acgcctcggc gatgtgccgg cgctcgatgc cggtgcggct gcggatccat
     6541 tcgtcgctcg tctccaggcg cgcggcgaac tcctggttcg tcatgacgcg cggcggcagg
     6601 aagctgccgg tgccggcgat gcgcgaatag atcaccgcgc caccgcctgc cgctcggcca
     6661 tgcgccgctc gatgcgctgc ggcacttcgt tgcgcacctc ctcgaccgcg cgcgagagcg
     6721 cctggccgaa cgcgaacacg tcggcagagc cgtggctctt gatgacgatg ccgcgcaacc
     6781 cgagcaggct cgcgccgttg tagcggcgat ggtccatgcg cccgcggaag gcgcgcagca
     6841 cgggtagcga gacgagagcg gccagccgcg tgaagggatt gcgcttgaac tcctcgcgca
     6901 aggtcgaggt catcatctgc accacgccct cggaagcctt cagcgccaca ttgccgacga
     6961 aaccgtcgca caccaccaca tcgatgttgc cccgatagat atcgtcacct tcgacgttgc
     7021 cggcgaagtt gagcccgctc gcgcgcagca actcggcggc ctgcttgacg gtttcgttgc
     7081 ccttgatgtc ttcgacgccg atgttgagca ggcccaccaa aggacgctcc ttgtgctcga
     7141 tcgccgccac cagcatcgcc cccatcatgc cgaactgcag caggttctcg gcggtgcaat
     7201 cgacgttcgc gccgaggtcg agcatgtagg tgttggtgcg acgcatgttg ggcagcacgg
     7261 tggcgatcgc gggccggtcg atccccggca gcgtcttcag cacgaagcgc gagatcgcca
     7321 tcagcgcgcc ggtgttgcc
//
LOCUS       DASPXW010000368         3152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1246325, whole genome shotgun sequence.
ACCESSION   DASPXW010000368 DASPXW010000000
VERSION     DASPXW010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246325"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VEL04_08530"
     CDS             <1..224
                     /locus_tag="VEL04_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HYT48453.1"
                     /translation="READLQRHLENSLAKGEPAARAKPVPQVVPGEQKPVELGSKDDF
                     QLSQALAFLKGEPVKSQSPTVAQNKPETN"
     gene            221..964
                     /locus_tag="VEL04_08535"
     CDS             221..964
                     /locus_tag="VEL04_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HYT48454.1"
                     /translation="MNDEQLLRYSRHILLPEIGIEGQERLRAASAFMVGAGGLGCPAA
                     LYLAAAGIGRLTIADADKVDLTNLQRQILYRTESVGAVKVEAAQATLNAVNPDVAVVA
                     LHKRIQDFSQLVADADVVLDCSDNFATRHALNRACVRLRKPLVSGAAMRFDAQLMVFD
                     LRNKNSPCYSCLFPEDAEVEEVQCSVMGVFAPLTGVIGAMQAMEAIKLVAGVGESLNS
                     RLQIFDAKSADWRSVKVAKDPGCAVCSKP"
     gene            complement(966..1523)
                     /gene="slmA"
                     /locus_tag="VEL04_08540"
     CDS             complement(966..1523)
                     /gene="slmA"
                     /locus_tag="VEL04_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HYT48455.1"
                     /translation="MPRAKGERRLEILKALAQMLEAPKWERITTAALAEKLGVSEAAL
                     YRHFASKAQMYEGLIEFIENSVFTLANKIASDEPDGRRQCEKLIEMLLAFAEKNPGMV
                     RVMTGDALVGEHERLQARINQFFERFESTLKQSLRAAAPDDDAPGRAGTLLRYSIGCL
                     HQFSKSGFAKKPTESFSAQRRYLLA"
     gene            complement(1551..2219)
                     /locus_tag="VEL04_08545"
     CDS             complement(1551..2219)
                     /locus_tag="VEL04_08545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HYT48456.1"
                     /translation="MRIARLPPSRRVWIFDLDNTLHDARPHIFPSMHGQINAYLRRRF
                     GVDDAGADAMRRDFWLRYGTTLRGLARHHGADPREFLRETHVFPQLAGMVVHESALRH
                     ALARLGGRKLVFSNAPRHYVEEVLRAIGIGRFFDAVYTIEDARYRGKPELHGFHRLLR
                     KHDLDPDRCAFVDDALENLRTAHRLGMSTVWVSRTQRRVPYIDLRISSITQLPRLAFR
                     YGEA"
     gene            complement(2219..3124)
                     /gene="argB"
                     /locus_tag="VEL04_08550"
     CDS             complement(2219..3124)
                     /gene="argB"
                     /locus_tag="VEL04_08550"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HYT48457.1"
                     /translation="MNAPLISSTAHSPQAAAKARILAEALPYIQRFHCKTIVVKFGGN
                     AMADNALKESFAYDVVLLKLVGMNPVVVHGGGPQIEQMLAKLGKKGEFIQGMRVTDGE
                     TMDIVEMVLGGQVNKEVVELINQAGGKAVGLTGQDGAFIRARKLMLPPSKENGSVDLG
                     QVGEVDSIDPSIIEALEQGGFIPVVAPIGTGADGTTYNINADLVAGKLAEILKAEKLV
                     VLTNTPGVLDKNGKLLTGLTPKKIDDLVEKGVISGGMIPKIGSALDAARNGVKSVHII
                     DGRVPHALLLEVLTDQGVGTLIRSK"
BASE COUNT          531 a         1028 c         1032 g          561 t
ORIGIN      
        1 tgcgcgaagc ggacctgcag cgccatctcg agaacagcct cgccaagggc gagccggcgg
       61 cacgcgccaa gccggtgccg caggtcgtgc ccggcgagca gaagccggtc gagctcggct
      121 cgaaggacga cttccagctc tcgcaggcgc tcgccttcct caagggcgag ccggtgaaga
      181 gccagtcgcc cacggtcgcg cagaacaaac ccgaaactaa ctgaacgacg agcagctcct
      241 ccgctacagc cgccatatcc tgctccccga aatcggcatc gaggggcagg aaaggctgcg
      301 tgcggcgagc gcgttcatgg tcggtgccgg cgggctcggt tgcccggcgg cgttgtatct
      361 cgcggcggcg ggcatcgggc gactgacgat cgcggacgcc gacaaggtcg acctcaccaa
      421 cctgcagcgc cagatcctct accgcaccga gtcggtcggc gcggtcaaag tcgaagcggc
      481 gcaggccacg ctcaacgccg tcaatcccga cgttgcggtg gttgctcttc acaagagaat
      541 tcaagacttt tcgcagcttg tcgcagacgc cgacgtcgta ctggactgct ccgacaactt
      601 cgccacgcgt catgcgctga accgggcgtg cgtccggctg cgcaagcccc tcgtctcggg
      661 cgccgccatg cgcttcgatg cccagctcat ggtcttcgac ctgcgaaaca agaactcccc
      721 ctgctactcg tgcctgtttc ccgaggacgc ggaagtcgag gaagtgcagt gctcggtgat
      781 gggcgtgttc gcgccgctca ccggcgtgat cggcgccatg caggcgatgg aagcgatcaa
      841 gctcgtcgcc ggcgtcggcg agtcgctcaa cagcaggcta cagatcttcg atgcaaagag
      901 cgccgactgg cgcagcgtga aggtggcgaa ggatccgggc tgcgcggtct gcagcaaacc
      961 ctgattcagg cgagcaggta gcgtctctgg gccgaaaagc tttcggtagg cttcttggcg
     1021 aagccgcttt tcgagaactg gtgcaggcag ccgatcgagt agcgcagcag cgtcccggcg
     1081 cgtccggggg catcgtcgtc cggcgccgcc gcgcgcagcg actgcttcag cgtcgattcg
     1141 aagcgctcga agaactggtt gatgcgcgcc tgcaggcgct cgtgctcgcc gaccagcgcg
     1201 tccccggtca tcacgcgcac catgccgggg ttcttctcgg cgaaggcgag cagcatctcg
     1261 atcagcttct cgcactgcct gcggccgtcg ggctcgtcgc tggcgatctt gttggcgagg
     1321 gtgaagaccg agttctcgat gaactcgatc agcccttcgt acatctgcgc cttcgaggcg
     1381 aagtggcgat acagcgccgc ctcggagacg ccgagcttct ccgccagggc ggcggtggtg
     1441 atgcgctccc atttcggcgc ctcgagcatc tgcgcgaggg ctttcaaaat ctccagccgg
     1501 cgttcgcctt tggcgcgggg catgtatgga taactgggga agcgcggatt ttatgcttct
     1561 ccgtagcgga aggccagacg cggcagctga gtgatggaag atattcttaa atcaatgtaa
     1621 ggcacgcgcc gctgcgtgcg gctcacccat accgtcgaca tgcccaggcg atgcgccgtg
     1681 cgcaggttct cgagcgcatc gtcgacgaag gcgcagcggt ctgggtccag atcgtgcttg
     1741 cgcagcagcc ggtggaagcc gtggagctcg ggcttgccgc ggtagcgcgc gtcctcgatg
     1801 gtgtacacgg catcgaagaa gcggccgatg ccgatcgcgc gcagtacctc ctccacgtag
     1861 tggcggggag cgttggaaaa caccagcttc ctgccgccga gccgcgccag cgcgtgcctg
     1921 agagcgctct cgtgcaccac catgccggca agctgcggaa agacgtgggt ttcgcgcagg
     1981 aattcgcgcg gatcggcgcc gtggtggcgt gcaagtcccc gcaatgtggt cccgtaccgc
     2041 agccagaaat cgcgccgcat ggcgtcggcg ccggcgtcat cgacgccgaa gcggcgcctc
     2101 agataggcgt tgatctgccc gtgcatcgac ggaaagatgt gtggccgggc gtcgtgcagc
     2161 gtgttgtcga ggtcgaagat ccacacccgc ctggacggcg gtaaccgcgc gatgcgcact
     2221 acttgcttct aatcagagtt cctacaccct gatcggtgag aacctccaga agcagcgcat
     2281 gcggcacgcg gccgtcgatg atgtgcacgc tcttcacgcc gttgcgcgcg gcgtcgagcg
     2341 ccgagccgat cttcgggatc atcccgcccg agatcacgcc cttctccacc aggtcgtcga
     2401 tcttcttcgg cgtgagcccg gtcaggagct tgccgttctt gtccagcacc cccggcgtgt
     2461 tggtgagcac caccagcttc tccgccttga ggatctcggc gagcttgccg gcgacgaggt
     2521 cggcgttgat gttgtaggtc gtgccgtcgg cgccggtgcc gatcggggcc accaccggaa
     2581 tgaagccgcc ctgctcgagc gcctcgatga tgctcgggtc gatcgaatcg acctcaccca
     2641 cctgtccgag gtcgacgctg ccgttctcct tggacggcgg cagcatcagc ttgcgcgcgc
     2701 ggatgaaggc gccgtcctgg ccggtgaggc cgaccgcctt gccgccggcc tggttgatga
     2761 gctcgacgac ttccttgttc acctggccgc cgagcaccat ctcgacgatg tccatggtct
     2821 cgccgtcggt gacgcgcata ccctgtatga actcgccctt cttgccgagc ttggcgagca
     2881 tctgctcgat ctgcgggccg ccgccgtgca ccaccaccgg gttcatgccg accagcttca
     2941 gcagcaccac gtcgtaggcg aagctttcct tcagcgcgtt gtcggccatc gcattgccgc
     3001 cgaatttgac gacgatggtc ttgcagtgga agcgctggat gtaaggcagc gcctcggcca
     3061 ggatgcgcgc cttcgcggcg gcctgcgggc tgtgagccgt ggaagaaata aggggggcgt
     3121 tcatcgcccc ccaatactac agaaaaacct ac
//
LOCUS       DASPXW010000369         6763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_484551, whole genome shotgun sequence.
ACCESSION   DASPXW010000369 DASPXW010000000
VERSION     DASPXW010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6763
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484551"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="VEL04_08555"
     CDS             complement(<1..897)
                     /locus_tag="VEL04_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014896040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HYT48458.1"
                     /translation="MQPLVISRFAIVNCLGAGTGALLDALRTKRSGLAPCDFETVALD
                     TWIGRVAAVEELRIRRGLEDYDCRSNRMAQLGLEQDGFTDAVAAARERYGAARIGVFI
                     GTSTSGILETELAYRCRDPQTRALPASFRYHATQNTYSLGDFVGRYLGLTGPSFVVSS
                     ACSSTAKVFGNAARMIAAGACDAAVVGGADTLCLTTLYGFYSLELTSSRPCRPFDADR
                     DGISLGEGAGFALLEKPTGPAASDAVMLLGVGESSDAYHMSTPHPEGAGAKLAMQRAL
                     QAARLGPADIDYINLHGTATRTN"
     gene            complement(879..1700)
                     /locus_tag="VEL04_08560"
     CDS             complement(879..1700)
                     /locus_tag="VEL04_08560"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HYT48459.1"
                     /translation="MTPDTYAAAGVYSGRRWRPAPAIRLSILLHGAGAISLGLHPDMW
                     PWVAATLIANHLVLLAAVFFPRGRLLGPNLNRLPASAVERGEVCLTFDDGPDPEVTPR
                     VLELLERYQAKASFFCVGERAQAHPEIVREIVRRGHSVENHSYGHSRAFALYGISLMR
                     REVESAQAAVTAITGRAPEFFRSPAGFRSPLLDFVLATAGLRYVSWTRRGFDAVSSDP
                     ARVLARLTPGLAAGDVLLLHDSAPVSLAVLPALLEQLAARGLKSVSLPGACSPSS"
     gene            complement(1697..2359)
                     /locus_tag="VEL04_08565"
     CDS             complement(1697..2359)
                     /locus_tag="VEL04_08565"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYT48460.1"
                     /translation="MRAIHDVAPGQDADILLVMLPAARARPEDLLEQGFVAAIRDRGL
                     PLDVAVVDAHADYYLEGRVGERLAVDVVTPLRAKGYRRIWLMGMSLGGLGCIAYAHKH
                     AAEVEGVILLAPFLGTRGLIAQILRAGGLARWQPGALEPDDEELGLLLWLKHYRADNP
                     RLPAIHLGFGRDDRYAPASAMLAQQLPAERVVSVPGKHDWQTWIRLWSALLAKNLFLV
                     EA"
     gene            complement(2343..4703)
                     /locus_tag="VEL04_08570"
     CDS             complement(2343..4703)
                     /locus_tag="VEL04_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008348286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HYT48461.1"
                     /translation="MNPAARWALGAWLAGVAACVAVIARTEFSTDLSAFLPRSPSPVQ
                     QVLVEQLRHGVVSRLILIGIEGDTPERLAQASKRLATSLRASGSFASVNNGEDAGFEN
                     DRDFVWRNRYLLSPTAEAGRFSPQALRESLEEALAQLASPAGVLLQRILPADPTGELI
                     RLLQQFAHQAQPRLQSGVWFSRDGGRALLIAQTRAAGSDIDAQERALALIRSSFEQAI
                     RADAQAAAGEAKLLLSGPGVFAVTTRGRIRHDAWRLSLIATTLIAAMLLALYRSVRVL
                     GLGLLPVVSGALAGIAAVSLSFGSVHGITLAFGVTLIGEGVDYAIYLFTQNAPGAAPQ
                     ATFELIWPTLRLGVLTSICGFSAMLFSDFTGLAQLGLFSVAGLVVAVAVTRWVLPALL
                     PVGFVVTAVAAFAPKVMGLVRAAPMLRRPLLVALAVTAAFLLMRPQPLWSGSLASLSP
                     VSPGEQALDQQLRQDIGAPEVRHLVVVHARDEQAALQASEHAAMALRESIAQGWLQGF
                     DSPAIYLPSRAMQRARQAALPAAETLRQSLQAALKGLPFRSNVFEPFVRDVSAARSMP
                     LLERSSLQGTSLALKLDSLLVKRESGWAAMLPLRGVTNVEAIAGKIGKPSDAQVVLLD
                     LKQEADQLYRTYLREALTNSLLGAAAIVILLFATLRSPRRVFDVLAPLAAAVLVSCGL
                     LVVSGIELSIFHLVGLLLVVAVGSNYSLFFDRQAPSGQDRSRTIVSLLFAAVSTLVGF
                     GLLAFSKVPVLSGIGSTVGMGAAMALVFSAIMSRKADTEGAYARNP"
     gene            complement(4700..5320)
                     /locus_tag="VEL04_08575"
     CDS             complement(4700..5320)
                     /locus_tag="VEL04_08575"
                     /inference="COORDINATES: protein motif:HMM:NF039988.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HYT48462.1"
                     /translation="MTTGNSFPLRHAIAALVIAAAAGGFPAAIAQTESKAKWDLAQLM
                     QDLRQVKAARGRFVERRYLAILNTPLESSGTLVYIAPGRLEKHMLLPRQESLTLDGDT
                     LLIENKETGQRRRVVLQEQPVVWAFVESIRATLAGDLQALNRFYEVSLEGDASRWRLR
                     LKPTEPRMREVASEIRVSGNGTWVGVIEILDAGGDRSVMTITRDAA"
     gene            complement(5304..6221)
                     /locus_tag="VEL04_08580"
     CDS             complement(5304..6221)
                     /locus_tag="VEL04_08580"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HYT48463.1"
                     /translation="MQRHVNGVAGRRPEWVERPERGSRLAVRLLLWLTLKLGRRVGRA
                     LLYPICAYFVLFSVRARRASAGYLRRVLGRRPGLGDVFRHYHAFASTVHDRIYLLAGK
                     YGYFDVTLSAPAELLARLRQGRGCILLGSHLGSFEILRARSVSDRVPPVNVLMHLDNA
                     EKINSVLHALAPGIESRIIPLGGPASLLRVRECLDRGEIVGVLGDRAWRNERSCSCDF
                     LGGQARFPLGPLLLAGLLEAPVVLCFGLYLGGRGYEVHLEPFADAIPLEPGGREAAVR
                     PWVERYARRLEHHCRLAPYNWFNFYDYWE"
     gene            complement(6197..6505)
                     /locus_tag="VEL04_08585"
     CDS             complement(6197..6505)
                     /locus_tag="VEL04_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48464.1"
                     /translation="MANLEHAFPSDHPATDGHFPGNPIIPGALLLSETLRLIEAHLGV
                     SLLPCRIRRAKFPHPARPGDRIAIEFASSSRHSIKFECAVKGNTVLVGEVECSDTSTV
                     "
     gene            complement(6507..>6763)
                     /locus_tag="VEL04_08590"
     CDS             complement(6507..>6763)
                     /locus_tag="VEL04_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-hydroxyacyl-ACP dehydratase"
                     /protein_id="HYT48465.1"
                     /translation="IGGVRDGAFFLPEDDDGAVARLIAFVVAPGLSHEAVMAALRQRI
                     DAAFLPRPLCMVDSLPRNETGKLPRVSLDTLATRLTTKAG"
BASE COUNT         1233 a         2311 c         2257 g          962 t
ORIGIN      
        1 attggtccgg gtcgccgtgc cgtggaggtt gatgtagtcg atgtcagccg ggccgagccg
       61 cgcggcctgc agcgcccgct gcatggcgag cttcgcgccg gcaccttccg gatgcggcgt
      121 cgacatgtgg tacgcatcgg agctctcgcc tacccccaga agcatgaccg cgtcggaagc
      181 cgccgggccg gttggctttt cgagcagcgc gaagcccgcg ccttctccca gcgagatgcc
      241 gtccctgtcg gcgtcgaagg gccggcacgg gcgactcgaa gtcagctcca gggaataaaa
      301 gccgtacagc gttgtcaggc acagcgtgtc cgcgccgccc actacggcgg catcgcaagc
      361 tcccgctgcg atcatgcgcg cggcgttgcc gaacaccttg gcggtggacg agcatgcgga
      421 agagacgacg aacgaaggcc cggtcaagcc gagatagcgc ccgacgaaat cgccgagcga
      481 ataggtgttt tgcgtcgcgt ggtagcgaaa gcttgccggc agagcgcgcg tttgcggatc
      541 gcggcagcga taggcgagct cggtttcgag aattccggag gtgctggtgc cgatgaagac
      601 gccgatgcgg gctgcgccgt agcgttcgcg ggccgccgcc acggcatcgg tgaacccatc
      661 ctgctcgagc cccagttgcg ccatccggtt gctgcggcaa tcgtagtctt cgaggccgcg
      721 ccggatgcgc agctcttcga ccgcggcaac ccgtccaatc caggtatcga gggcgacagt
      781 ctcgaaatcg cacggcgcca gcccggatcg cttcgtgcgc aaggcgtcga gcagcgctcc
      841 ggtcccggct cccaggcagt tgacgatcgc aaaccggctt atgacgaggg gctgcatgcg
      901 cccggcaggg aaacggactt gagccctcgc gcggccagct gctcgagcag cgcgggcagg
      961 acggcgagcg agaccggcgc gctgtcatgc aacagcagca cgtcgccggc ggcgagcccg
     1021 ggggtgagac gcgccagcac gcgcgcggga tcgctgctga cagcgtcaaa gccacggcgc
     1081 gtccatgaga cgtagcgcaa tcctgccgtc gccaggacga agtcgagcaa cgggctgcgg
     1141 aaacccgccg gagaacggaa gaattcggga gcccgtccag taatggcggt gacggcggct
     1201 tgcgccgact ctacctcacg ccgcatcagg gaaatgccgt acagggcaaa cgcgcgggaa
     1261 tggccatagc tgtggttctc gacgctgtga ccgcggcgca cgatttccct gacgatctcg
     1321 ggatgggcct gtgccctttc cccgacgcag aagaagctcg ccttggcctg gtaacgctcc
     1381 aggagctcga gcacccgggg cgtcacctcg ggatccgggc catcgtcaaa cgtcagacac
     1441 acttccccgc gctcgacggc cgaggccggc aggcggttga ggttcggtcc cagcaagcgc
     1501 ccgcgcggga aaaatacggc ggcgagcaat accaggtggt tggcaatcaa ggtagccgcg
     1561 acccaaggcc acatatccgg gtgcagcccg agcgaaatgg caccggcccc atgcagcagg
     1621 atggaaaggc ggatggcggg cgccggacgc cagcggcggc cgctgtagac gccggcggct
     1681 gcgtaggtgt cgggtgtcat gcttctacca gaaacaggtt cttggccagc agcgcgctcc
     1741 acaagcgaat ccaggtctgc caatcatgct tgccgggaac gctcaccacg cgttctgccg
     1801 gcaattgttg agcgagcatc gcacttgccg gcgcatagcg gtcatctctg ccgaatccca
     1861 gatggatcgc gggcaatcgc ggattgtctg cccggtagtg cttcaaccac aacagcaatc
     1921 ccagctcctc gtcgtccggc tcgagggctc ccggctgcca tcgagcgagg ccgcccgcgc
     1981 gcaggatctg cgctatcaga ccgcgcgtgc cgagaaatgg cgcgagcagg atcacgccct
     2041 cgacctcggc cgcatgcttg tgcgcatagg cgatgcatcc caggccgccc agcgacatgc
     2101 ccatgagcca gatccgccgg tagcccttgg cgcgcagcgg cgtgacgaca tcgaccgcga
     2161 ggcgctcgcc gaccctgccc tcaagatagt agtccgcgtg agcatccacg acggcgacgt
     2221 cgagcggcaa tccacgatcg cgtatcgccg cgacgaagcc ctgctccaac aggtcttccg
     2281 ggcgcgccct ggcggccggc agcatgacga gcaggatgtc ggcgtcctgg ccgggcgcca
     2341 cgtcatggat tgcgcgcata agcaccttcg gtgtccgcct ttcggctcat gatcgccgaa
     2401 aacaccagcg ccatggccgc ccccatgccc acggtcgagc cgatgccgct cagcaccggc
     2461 accttcgaga acgcaagcag gccgaagccg accagcgtgg atacggctgc aaacagcagc
     2521 gacacgatgg tgcggctgcg gtcctggccg gaaggcgcct gccgatcgaa gaacagcgaa
     2581 tagttggatc cgaccgccac caccagcagc aggcccacca gatggaagat cgaaagctcg
     2641 atgccgctca cgacgagcag cccgcaactc accaggacag cggcggcgag cggtgccagc
     2701 acgtcgaaca cgcgtcgcgg cgagcgcagg gtcgcaaaca gcaggatgac tatcgcggca
     2761 gcgcccagca gcgagttcgt cagggcctcc cgcagatagg tccggtagag ctggtcggcc
     2821 tcttgcttca ggtccagcag cacgacctgg gcatcggagg gcttgcctat cttgccggcg
     2881 atggcttcga cgttggtcac gccccgcaag ggcagcatgg ccgcccaccc ggattcgcgc
     2941 ttcaccagca gcgagtcgag cttgagcgcg aggctcgtgc cctgcaggct cgagcgctcg
     3001 agcagcggca tgctcctcgc cgcggatacg tcccgcacga aaggctcgaa gacattggag
     3061 cgaaaaggca gccccttgag cgccgcctgc aggctctggc gcaatgtttc cgccgccggc
     3121 aatgcggcct ggcgggcgcg ctgcatcgcc cggctcggca ggtagatggc gggtgaatcg
     3181 aagccttgca gccagccctg cgcgatggat tcgcgcaacg ccatggcggc gtgctcgctc
     3241 gcctggagcg cagcctgctc gtctcgcgcg tggaccacca cgagatggcg cacctcgggg
     3301 gcgccgatgt cctggcgcag ctgctggtcg agcgcctgct cgcccggcga cactggactg
     3361 aggctcgcga ggctgccgct ccacagcggc tgcgggcgca tgagcagaaa cgcagccgtg
     3421 accgcgagcg cgaccagcag ggggcggcgc agcatgggcg cggcgcgcac caggcccatc
     3481 accttcgggg cgaatgccgc taccgcggtc acgacaaatc ccacgggcag caacgcgggc
     3541 aggacccagc gggtcactgc gacggcgacc accagcccgg ctaccgaaaa cagcccgagt
     3601 tgcgccaggc ccgtgaaatc ggaaaacagc atcgcgctga agccgcagat cgaggtgagc
     3661 accccgagcc gcaaggtcgg ccagatcagc tcgaaagtgg cttgcggcgc cgctcccggc
     3721 gcgttctgcg tgaagaggta aatcgcgtag tccacgcctt cgccgatcag ggtcacgccg
     3781 aaagcgagcg tgatgccatg cactgagccg aaactcaggc tcacggccgc gatgccggca
     3841 agcgccccgc tcaccacggg gagcaggccg agcccgagca cgcgcaccga gcggtatagc
     3901 gccagcagca tggcggcgat cagggtggtg gcgatcaggg acaggcgcca ggcgtcgtgc
     3961 ctgatgcgcc cgcgggtggt gaccgcgaaa acgcccgggc cgctcaacag cagcttcgcc
     4021 tcgcctgccg ctgcttgggc gtctgctcgt atggcctgct cgaatgagct ccggatcaag
     4081 gccagcgcgc gctcctgcgc gtcgatgtcg gagcccgcgg cgcgggtttg cgcaatgagc
     4141 agcgcccggc cgccgtccct ggagaaccag acccccgact gcagcctcgg ctgagcctga
     4201 tgcgcaaact gctgcaacag gcggatgagc tcgccggtgg gatccgcggg cagaatacgc
     4261 tgcaacagca cgccggcagg cgaagccagc tgcgcgagag cctcctcgag gctctcgcgc
     4321 agcgcctgcg gcgaaaaacg cccggcttct gccgtgggac tcaaaaggta gcgattgcgc
     4381 cagacaaaat cccggtcgtt ctcgaagcct gcatcctcac cgttgttcac cgatgcaaag
     4441 ctgcccgagg cgcgtaatga cgtcgcgagc cgcttgctcg cttgcgccag ccgctccggg
     4501 gtatcgcctt cgatgccgat caggatcaag cgtgacacca cgccatggcg cagctgctcg
     4561 accagcacct gctggacggg cgaaggcgaa cgcggaagaa aagcggaaag atcggtgctg
     4621 aactcggtgc gtgcgatgac ggcaacgcag gcggcgacgc ccgcgagcca tgcgccgagc
     4681 gcccagcgcg ctgccgggtt cacgctgcgt cccgagtgat ggtcatcacc gagcgatcac
     4741 cgccggcatc gagaatctcg atcacgccca cccaggtgcc gttgccgctg acgcggattt
     4801 cgctggccac ctctcgcatg cgcggctccg tgggcttgag ccgcaggcgc caccggcttg
     4861 catcgccttc caggctgacc tcgtagaagc ggttcagggc ctgcagatcg cccgccagcg
     4921 tcgcacggat gctctcgacg aaggcccaga ccaccggctg ctcctgcagc acgaccctgc
     4981 gccgctgccc ggtctccttg ttttcgatca gcagcgtgtc gccgtccagg gtcaggctct
     5041 cctgccttgg caacagcatg tgcttctcga gccgcccggg cgcgatgtac accagcgtcc
     5101 cggaagattc aagcggcgtg ttgaggatcg cgagatatct gcgctcgaca aacctgccgc
     5161 gcgccgcctt cacctgccgc aggtcctgca tcagctgcgc cagatcccat ttggctttgc
     5221 tctccgtctg cgcgatcgcg gccgggaagc cgcccgccgc ggcggcgatg acgagcgccg
     5281 cgatcgcatg acgcagcgga aagctattcc cagtagtcat agaagttgaa ccagttatac
     5341 ggcgcgaggc gacagtggtg ctccagccgg cgggcgtagc gctccaccca cggccgcacc
     5401 gccgcttcgc gcccgcccgg ctcgagcggg atcgcatcgg cgaaaggctc aaggtgaacc
     5461 tcgtagcccc ggccgcccag gtacaggccg aagcacagga ccaccggcgc ctccagcagc
     5521 ccggcgagca gcagcggacc gagcggaaag cgcgcctgcc cgccgaggaa gtcgcagctg
     5581 cagctgcgtt cgttccgcca ggcccggtcg ccgagcacgc cgacgatctc tccgcggtcg
     5641 aggcattcgc gcacccgcag cagcgatgcc ggaccgccaa gcgggatgat ccgcgactcg
     5701 atgcccggag cgagcgcatg cagcacgctg ttgatctttt cggcgttgtc gagatgcatc
     5761 agcacgttca ccgggggcac gcggtcggaa acgctgcgcg cgcgcaggat ctcgaagctg
     5821 cccaggtgcg acccgagcag gatgcagccg cggccctgcc gcagccgcgc cagcagctcg
     5881 gccggggccg acagcgtcac gtcgaagtag ccgtacttcc ctgccaggag atagatgcga
     5941 tcgtgaaccg tcgaggcgaa ggcatgatag tgacgaaaca catcgccgag cccagggcgc
     6001 ctgccaagaa cgcgccgaag gtatccggcc gaagcgcgcc gcgcgcgcac cgagaacagc
     6061 acgaagtagg cgcagatcgg atacagcaag gcgcgtccca cgcgccggcc gagcttcagc
     6121 gtcagccata acagcaggcg caccgcgagc ctgctgccgc gctcgggacg ctcgacccac
     6181 tcgggtcgcc ggcctgctac accgttgacg tgtcgctgca ttcgacttcg cccaccagca
     6241 ccgtatttcc cttcaccgcg cattcgaact tgatcgagtg gcgggacgag ctggcgaact
     6301 cgatcgcgat acggtctccc ggccgagccg ggtgaggaaa cttcgcccgc ctgatgcggc
     6361 acggcaaaag gctcacgccg agatgcgcct cgataaggcg tagcgtctcg ctcaggagga
     6421 gcgcgcccgg aatgatcggg ttgcctggaa agtgcccgtc ggtcgctgga tggtcggaag
     6481 gaaaagcgtg ctcgaggttg gccaaactag cctgccttgg tcgtcagccg cgtcgccaga
     6541 gtatcgagcg acacccgcgg cagcttgccg gtttcgttgc gcggcaatga atccaccatg
     6601 cacagcgggc gcggaagaaa agccgcgtcg atccgctggc gcagcgcagc catgacggcc
     6661 tcgtggctga ggcccggcgc caccacgaaa gcaatcagcc gcgccactgc gccgtcgtcg
     6721 tcttccggca ggaaaaatgc gccgtcgcgg acgccgccga tcg
//
LOCUS       DASPXW010000370         2092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1119024, whole genome shotgun sequence.
ACCESSION   DASPXW010000370 DASPXW010000000
VERSION     DASPXW010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1119024"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VEL04_08595"
     CDS             <1..223
                     /locus_tag="VEL04_08595"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HYT48466.1"
                     /translation="MQTLKAAGASPKDVVHVFIFRARPRMGDIGKASAVVNSYFAPYN
                     HKPTTTNLAVLELGEPEQLIEIQVVAVVD"
     gene            complement(237..>2092)
                     /locus_tag="VEL04_08600"
     CDS             complement(237..>2092)
                     /locus_tag="VEL04_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48467.1"
                     /translation="APGGTRWYVQADGYTRPKLMRQTGSAAPEVVRELEQDTRLTATA
                     GDAVLASELDICRNHNLLYDLYEVAADGRRKRLTDCGRYRFAAPLDDGRIAALRVSAG
                     ETEVVVLDPRGAVERALYRAAQGESLSGLAARAGTVVVTSLRDGRWSLLDVTGGKVTA
                     LLSDASIKHSPRFGDSPEEIFFIADYGKVYNVWSLERASGTLSRWTRASNGVREISAP
                     LGGQVLLTNIEADGDALRLYRLPDAPLEERKAAAEAAPPAPRAESAVAGADRPYSPWS
                     SLRPRSWLPQLEIADGAFAVGAVTYGQDALDLHEYFVAPLYEITQRQLLGSAAYVYEG
                     RHSLLVNRTMTVKASETRNNSREITTYSIKENAQWVSTWRHLALNQRFYWGLGAALEQ
                     EKLHFVDDGTSPVQNERVLGLVAGVDTRRAQWLSEGPSQGQWLRLFAETSSRLNAAYT
                     GNVYRADWRGHLALGHSVLALRWNEAYGQPGAEPFQLGGSRSDDWIPLPVLNQREFAL
                     RGYTNGEPVLTGHRARVVTTEWRMPLADVDRHSMTPPLGLNRVSLNLFLDVGAAWERG
                     GDPDYHRGVGVELITEPRAAYVFEWQLRAGVAKGLDAPGTTKFYLRRGRSF"
BASE COUNT          330 a          716 c          717 g          329 t
ORIGIN      
        1 catgcagacg ctgaaggcgg cgggcgcatc gcccaaggac gtggtgcacg tgttcatctt
       61 ccgcgcgcga ccccgcatgg gcgacatcgg caaggcgagc gcggtggtga actcctactt
      121 cgcgccctac aaccacaagc cgacgacgac caacctcgcc gtcctcgagc tcggggagcc
      181 cgagcagctg atcgagatcc aggtggtcgc cgtagtggac tgaggcgggt ttgcggtcag
      241 aacgaccgcc cccgcctgag ataaaacttc gtcgtccccg gggcgtcgag gcctttcgcg
      301 acaccggcgc gcagctgcca ttcgaacacg taggcggcgc gcggctcggt gatcagctcg
      361 acgccaacgc cgcggtggta gtcggggtcg ccgccgcgtt cccacgccgc tccgacgtcg
      421 aggaacaggt tgagcgaaac gcggttcagt cccagcggcg gggtcatgga atgccggtcg
      481 acatcggcga gcggcatgcg ccattccgtg gtcaccacgc gggcgcggtg cccggtgagc
      541 accggctcgc cgttggtgta gccgcgcagc gcaaattcgc gctgattgag caccggcagg
      601 ggaatccagt cgtccgacct gctgccgccg agctggaacg gctcggcgcc cggctgcccg
      661 taggcctcgt tccagcgcaa ggccagcacc gagtgcccca gcgcgaggtg accccgccag
      721 tcggcgcggt acacatttcc ggtataagcg gcgttgagcc ggctcgaggt ctccgcgaac
      781 agccgcagcc actgcccctg gctcgggcct tccgaaagcc actgcgcgcg ccgcgtatcg
      841 actccggcca ccagccccag tacgcgctcg ttctgcaccg gcgaagtgcc gtcgtcaacg
      901 aagtggagct tctcctgctc gagcgccgcg ccaagccccc agtagaagcg ctggttgaga
      961 gcgaggtggc gccacgttga cacccactgc gcgttctcct tgatgctgta agtggtgatc
     1021 tcgcggctgt tgttcctcgt ctcgctcgcc ttgaccgtca tcgtgcggtt gaccagcagg
     1081 ctgtgacgcc cctcgtagac gtaagcggcg ctgccgagaa gctggcgctg ggtgatttcg
     1141 tacagcggcg cgacgaagta ctcatgcaga tccaatgcgt cctggccgta ggtgacggca
     1201 cccaccgcga aagcgccgtc ggcgatttcg agctggggca gccatgagcg aggccgaagc
     1261 gatgaccacg gcgaataggg ccggtcggcg cccgctaccg cggattcggc gcgcggcgca
     1321 ggcggagccg cttcggccgc cgctttgcgc tcctcgagcg gcgcatccgg gagccgatac
     1381 agccgcagcg cgtcgccgtc ggcctcgatg ttggtgagca gcacctggcc gccgaggggc
     1441 gcgctgattt cgcgcacgcc attgctcgcc cgcgtccagc gcgacaacgt gccgctcgcg
     1501 cgctcgagcg accagacgtt atagactttg ccgtagtccg cgatgaagaa gatctcctcc
     1561 ggcgagtcgc cgaagcgcgg ggaatgcttg atggacgcat cggacagcag ggccgtgacc
     1621 ttgccgccgg tgacgtcgag aagcgaccag cggccgtcgc gcaggctcgt tacgaccacc
     1681 gttccggccc tggcggcgag gccgctcagc gattcgcctt gcgccgcgcg gtagagagcg
     1741 cgctcgaccg cgccgcgcgg gtcgagcacc accacttccg tttcgccggc tgagacccgc
     1801 aaagcggcga tacggccatc gtcgagcggt gcggcgaagc gatagcggcc gcagtccgtg
     1861 agtcgcttgc gcctgccatc ggcggcgacc tcatagagat cgtagagaag gttgtggttc
     1921 ctgcagatat cgagctcgga cgccaggacc gcgtcccctg cagtcgcggt gagcctggtg
     1981 tcctgctcga gttcgcggac cacctcgggg gcggcgctgc cggtctgacg catgagcttc
     2041 ggccgcgtgt agccgtccgc ctggacatac cagcgggtcc cgcccggcgc ga
//
LOCUS       DASPXW010000371         5659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_485777, whole genome shotgun sequence.
ACCESSION   DASPXW010000371 DASPXW010000000
VERSION     DASPXW010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5659
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485777"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VEL04_08605"
     CDS             <1..199
                     /locus_tag="VEL04_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYT48468.1"
                     /translation="DDAKLYGKKVLVACGSEDVITPEAGCKAVATAFPKAAYRSLAGL
                     GHAPHVEDPARINAMIREFVR"
     gene            complement(230..1180)
                     /locus_tag="VEL04_08610"
     CDS             complement(230..1180)
                     /locus_tag="VEL04_08610"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYT48469.1"
                     /translation="MRKSIAALVLLLVSLSAAAQYPDKPVTMLAGYPPGGLVDVVARL
                     VAEGTKQKFPRGLTVVNRPGAAGSVAVAEMARSSPDGYTIVLTPHSALVIAAQIQDLA
                     YKTPDDYDPFINLVSYYPMIAVRTESEYRTIQELIADAKANPGKIRVGSPGEGTSSHL
                     NLEELMHHAGVKMIHVPFQGWGQSSPALLGGHIEALVAQPGELKGQIDGKRMRVLVAF
                     QPRRHPVFPDVPTAKELGWDVANGVWYMLMAPKGTPVQVIRYLHDAAKAAIEDPKFAA
                     NMAGRGIDVDYRPGPALRADLWREYKLHTEILRRIGMLKR"
     gene            1218..2006
                     /locus_tag="VEL04_08615"
     CDS             1218..2006
                     /locus_tag="VEL04_08615"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HYT48470.1"
                     /translation="MELNERQLAQFRRDGYLIFPELFSKAEIAVLRAETARLATLETE
                     TVIRERTGGVRSIFRVHEDDGATRSAAFHALVRTPRVLRPTMQALGSDQVYVYHTKIN
                     TKPAIEGTVWMWHQDYGSWQRDGCPRPDMGTFAVMLTDSVEMNGALYVIPGSHQRGRI
                     EPYYDESTSYKFWAVPKKEIIALLKESDPPVPIVGPAGTAVLFHCNLLHASGHNLSAE
                     DRWHVYISFNSCANAPRFGPNSRPDWVVSRNTKPLALEKDALAA"
     gene            complement(2012..3673)
                     /gene="ettA"
                     /locus_tag="VEL04_08620"
     CDS             complement(2012..3673)
                     /gene="ettA"
                     /locus_tag="VEL04_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HYT48471.1"
                     /translation="MAQYVYTMRRVGKVVPPKRAILKNISLNFFPGAKIGVLGLNGSG
                     KSSLLKIMAGVDDHYEGEVVRMPGISVGYLEQEPRLPQGKSVRQAVEEAVGETAVAKK
                     RLEEIYAAYAEPGADFDQLAEEQAKLEAMINTGGADELEVAADALRLPPWDAEVDVLS
                     GGEKRRVALCRLLLSKPDLLLLDEPTNHLDAESVEWLEQFLQRFPGTVVAVTHDRYFL
                     DNAAGWILELDRGYGIPWEGNYSSWLEQKQKRLQVEASQEAARIKAMQSELEWVRQNP
                     KGRQSKSKARLARFEELSSQEHQARNETQEIYIPVAERLGNEVIELQDVSKGYGDRLL
                     IDKLSFKVPPGAIVGIIGPNGAGKSTLFKLMTGKEKPDAGKIRIGKTVKLAHVDQSRD
                     VLQADKSVFEDVSGGNDMLAVGKFQMPSRAYLARFNFRGADQQKPVGQLSGGERGRLH
                     LAKMLISGANVLLLDEPSNDLDVETLRALEEALLEFAGSVLVISHDRWFLDRIATHIL
                     ASEDDSQWVFFNGNYQEYEADKKKRLGEEAARPHRIRYKPLSRAK"
     gene            3696..4220
                     /locus_tag="VEL04_08625"
     CDS             3696..4220
                     /locus_tag="VEL04_08625"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HYT48472.1"
                     /translation="MHKKLAAAVCCAILCFATPARAVDGLSLEIGQGHGLWQWRAGAQ
                     WNWPQKSMLDAGEWHTRGYWDLAAGVWEGGVDTIYDLGLTPVLRMERGEYSSPYLEAG
                     IGAHMLSSLHITPYRTFSSHLQFGDHIGVGLRFGYEGRFDIGLRLQHLSNAGLRNPNP
                     GINFLQMRLQYHLN"
     gene            complement(4225..5448)
                     /locus_tag="VEL04_08630"
     CDS             complement(4225..5448)
                     /locus_tag="VEL04_08630"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HYT48473.1"
                     /translation="MRRLELFALAFAAGPAWAQLPATVTLDEALSIVARSPRVVAAER
                     DADAARAERVTAGALPNPNFSLGTSRLAGGERTVFDANSQQQATVELPIPVFGQREAR
                     VRAAERQVGRADSQVRLTVSETRRQAALEFARLLQAQEGLAARRAALGEIERIRGLVS
                     GRLESGMASRYDLARADAELALAGVAVQRAEAEVSGHAAALAALADASGWRPRAAGAL
                     QAMQVGPGSQPDAEAMLAANPAARVARDDSAAAEARIELARRERLPVPSVSFGRSWTS
                     GPFGAANFIGLSSEIPILDSKRGLEDKARADAAAARERERAANAALRAEYQKQRDALQ
                     VRRDALRRFDADVSGRQGAFLEMAESAYRLGRGTLFELLDARRTQLEAAVARLELLGA
                     IVEAELELRALAGEL"
     gene            complement(5435..>5659)
                     /locus_tag="VEL04_08635"
     CDS             complement(5435..>5659)
                     /locus_tag="VEL04_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HYT48474.1"
                     /translation="KLRAVLMAALLALFGLLPMALSTGIGSETQRPFALVIVGGMVST
                     LVITLCVLPAVYYALAAEKYTTPEELDETA"
BASE COUNT          898 a         1951 c         1858 g          952 t
ORIGIN      
        1 cgacgacgcg aagctctacg ggaagaaagt gctggttgcc tgcggcagcg aagacgtcat
       61 cactccggaa gcgggctgca aggcggtcgc cacggcattt ccgaaggccg cataccgctc
      121 gctcgccggc ctcggccatg cgccacatgt cgaggacccg gcgaggatca acgcgatgat
      181 cagggaattc gtgcgatagg tggatgaact tataaagcta taagctcact caacgcttga
      241 gcataccgat gcggcgcagg atctcggtat ggagcttgta ctcgcgccac agatcggcgc
      301 gcagcgcggg gcccgggcga tagtcgacgt cgatgccgcg cccggccatg ttggccgcga
      361 acttcgggtc ctcgatcgcc gccttggcgg cgtcgtgcag gtagcggatc acctgcaccg
      421 gcgtgccctt cggcgccatc agcatgtacc agacgccgtt ggcgacgtcc cagcccagct
      481 ctttcgcggt cggcacatcg gggaacaccg ggtggcgcct gggctggaat gcgaccagca
      541 cgcgcatgcg cttgccgtcg atctggcctt tgagctcgcc cggctgcgca accagcgcct
      601 cgatgtgccc gccgagcagc gccgggctcg actggcccca gccctggaac ggcacgtgga
      661 tcatcttcac gccggcgtgg tgcatcagtt cttccaggtt gaggtggctc gaggtgccct
      721 cgcccggcga gccgacgcgg atcttgcccg ggttcgcctt ggcgtcggcg atcaattcct
      781 ggatggtcct gtattcggat tcggtgcgta ccgcgatcat cgggtagtac gagaccaggt
      841 tgatgaacgg gtcgtagtcg tcgggcgtct tgtaggcgag gtcctggatc tgcgccgcga
      901 tgaccagcgc cgaatgcggc gtcagcacga tggtgtagcc gtcgggcgag ctgcgcgcca
      961 tctcggcgac cgccaccgag cccgccgcgc ccggccggtt caccaccgtc agcccgcgcg
     1021 gaaatttctg cttcgtgcct tcggcgacga gccgcgccac gacgtcgaca agtcccccgg
     1081 gcggatagcc ggcgagcatg gtcaccggct tgtctggata ttgcgccgca gcggacagcg
     1141 acaccagcag caaaaccagc gccgcaatgc ttttgcgcat ggcgagctcc tcctcgcggc
     1201 gatgttagca tcggacgatg gaattgaatg agcggcagct cgcgcagttc cggcgcgacg
     1261 gctacctgat cttcccggag ctgttctcga aggccgaaat cgccgtgctc agggccgaaa
     1321 ccgcccgtct cgccaccctt gaaacggaaa ccgtcattcg cgagcgcacc ggcggcgtgc
     1381 gctcgatctt ccgcgtgcac gaggacgacg gcgcgacgcg ctcggcggcg ttccacgccc
     1441 tggtgcgcac gccgcgcgtg ctgcgcccga cgatgcaggc gctcggtagc gaccaggttt
     1501 acgtctacca caccaagatc aacaccaagc ccgcgatcga gggcacggtg tggatgtggc
     1561 accaggacta cggctcgtgg cagcgcgacg gctgcccgcg ccccgacatg ggcaccttcg
     1621 ccgtgatgct gaccgactcg gtcgagatga acggcgcgct gtacgtcatc cccggcagcc
     1681 accagcgcgg ccgcatcgag ccgtactacg acgagagcac gtcgtacaag ttctgggccg
     1741 tccccaaaaa ggaaatcatc gcgctattga aggaatcgga ccctcccgtt ccaatcgtcg
     1801 gtcctgccgg cactgccgtt ctatttcatt gcaatctgct gcacgcctcg ggccacaacc
     1861 tcagcgccga agaccgctgg cacgtttata tctcgttcaa ttcctgcgcc aacgcgccgc
     1921 gctttggacc caattcgcgg ccggactggg tcgtttcccg gaataccaag ccgcttgctt
     1981 tggaaaaaga cgcgctggcg gcctaagcgg tctatttggc tcgggacagg ggcttgtagc
     2041 ggatgcggtg cgggcgcgcg gcctcctcgc cgaggcgctt cttcttgtcg gcctcgtatt
     2101 cctggtaatt gccgttgaag aacacccact gcgagtcgtc ctcgctggcg aggatgtggg
     2161 tggcaatgcg gtcgaggaac cagcggtcgt gcgagatcac cagcactgag ccggcgaatt
     2221 ccagcagcgc ctcctcgagc gcgcgcagcg tctcgacgtc gagatcgttg gagggttcat
     2281 ccaggagcag gacgttggcg cctgagatca gcatcttggc gagatgcagc ctgccgcgct
     2341 cgccgcccga gagctggccg acgggcttct gctggtcggc gccgcggaag ttgaagcgcg
     2401 cgaggtaggc gcgcgagggc atctggaatt tgcccactgc cagcatgtcg ttgccgccgg
     2461 agacatcctc gaacacgctc ttgtccgcct gcagcacatc gcgcgactgg tcgacgtgcg
     2521 caagcttcac ggtcttgccg atgcggatct tccccgcatc cggtttttct ttccccgtca
     2581 tcaatttgaa cagcgtggac ttgccggcgc cattgggccc gatgatgccg acgatcgcgc
     2641 cgggcggcac cttgaacgac agcttgtcga tcagcagccg gtcgccgtaa cccttggaga
     2701 catcctgcaa ctcgatcacc tcgtttccca ggcgctcggc gaccgggata tagatctcct
     2761 gggtctcgtt ccttgcctga tgctcctgcg aggagagctc ctcgaaccgg gcgaggcgcg
     2821 ccttggactt cgactggcgg cccttcgggt tctgccgcac ccattcgagc tcggactgca
     2881 tcgccttgat gcgcgctgct tcctggctcg cctcgacctg gaggcgcttc tgcttctgct
     2941 cgagccagct cgagtagttg ccttcccagg ggatgccgta accgcggtcg agctcgagga
     3001 tccagccggc ggcgttgtcg aggaagtagc gatcatgggt cactgcgacc acagtgccgg
     3061 ggaagcgctg caggaactgc tccagccact cgacgctttc ggcgtcgagg tggttggtgg
     3121 gctcgtccag cagcagcaga tcgggtttcg acaaaagtaa tctacaaagg gcaacgcggc
     3181 gcttttcgcc tcccgagagg acatcgacct cggcatccca gggaggcagc cgcaatgcgt
     3241 ccgccgcgac ctccagctcg tcggcgccgc cggtgttgat catcgcctcg agcttcgcct
     3301 gttcttccgc gagctggtcg aaatccgcgc ccggctccgc ataagcggcg tagatctcct
     3361 ccaggcgctt tttcgccacc gcggtctcgc cgaccgcttc ttcaaccgct tgcctgaccg
     3421 atttcccctg gggaaggcga ggttcctgtt ccaggtagcc gacgctgatg cccggcatgc
     3481 gcaccacctc gccctcgtag tggtcgtcga cgccggccat gatcttcagc agcgacgact
     3541 tgcccgagcc gttcagaccg agcacgccga tcttcgcgcc gggaaaaaag ttgagcgaga
     3601 tgttcttcag gatggcgcgc ttgggcggca ccacctttcc aaccctgcgc atggtgtaaa
     3661 cgtactgggc catggcgcta tgatagggaa agaagatgca taaaaaactc gctgctgcgg
     3721 tctgctgcgc gatcctctgc ttcgcaacgc ctgcccgcgc ggtcgacggc ctgtcgctcg
     3781 agatcggcca gggccacggc ctgtggcagt ggcgcgccgg cgcgcagtgg aactggccgc
     3841 agaaaagcat gctggacgcc ggcgagtggc acacccgcgg ctactgggac ctcgccgccg
     3901 gggtctggga aggcggcgtc gacaccatct acgacctcgg cctgaccccg gtgttgcgca
     3961 tggagcgcgg cgagtactcg agcccttacc tggaggccgg gatcggcgcg cacatgcttt
     4021 cgagcctgca catcacgccc taccgcacct tcagcagcca cctccagttc ggcgaccata
     4081 tcggcgtcgg gctgcgcttc ggctacgaag gccgcttcga catcggcttg cgcctgcagc
     4141 acctttccaa cgctggcctg cgcaatccca atccgggaat caactttctg cagatgcggc
     4201 tgcagtacca cctcaattga gccgctacaa ctccccggcg agcgcgcgca gctcgagctc
     4261 ggcttcgacg atcgcaccaa gcagctcgag ccgggcgacc gccgcctcga gctgcgtgcg
     4321 gcgcgcatcg agcagctcga acagcgtgcc ccggcccagg cggtaggcgc tttccgccat
     4381 ctcgagaaac gccccctggc gcccggatac gtcggcgtcg aagcggcgca gggcatcgcg
     4441 gcgcacctgc agcgcgtcgc gctgcttctg gtattcggcg cgcagcgccg cattcgcggc
     4501 gcgctcgcgc tcgcgggcgg ccgcggcgtc ggcgcgcgcc ttgtcctcga gcccgcgctt
     4561 gctgtcgagg atcgggatct cgctcgacag gccgatgaaa ttcgccgcgc cgaacggccc
     4621 gctggtccag ctgcggccga aggagacgct cggcaccggc aggcgctcgc ggcgcgccag
     4681 ctcgatgcgc gcttcggcgg ccgcggaatc gtcgcgcgcc acgcgcgccg ccgggttggc
     4741 ggcgagcatc gcctcggcat cgggctgcga tccgggcccg acctgcatcg cctggagcgc
     4801 gccggccgcg cgcggccgcc agccggaggc atcggcgagc gcagcgagcg ccgcagcatg
     4861 cccactcact tccgcctcgg cgcgctgcac ggcaacgccg gcgagcgcca gctcggcatc
     4921 ggcgcgcgcc aggtcgtagc ggctcgccat cccgctctcc agccgtcccg agaccagccc
     4981 gcgaatgcgc tcgatctcgc cgagcgccgc gcgccgcgcc gcgaggccct cctgcgcctg
     5041 cagcagccgg gcgaactcga gcgccgcctg gcgccgcgtc tcgctcaccg tgaggcggac
     5101 ctgcgaatcg gcgcggccga cctggcgctc ggccgcgcgg acgcgcgctt cgcgctggcc
     5161 gaataccggg atgggcagct cgacggtcgc ctgctgctgg gagttggcgt cgaacacggt
     5221 gcgctcgccc ccggccagcc gcgaggtgcc gagcgaaaag ttcggattgg gcagcgcgcc
     5281 ggcggtgacg cgctcggcgc gcgcggcgtc ggcgtcccgc tcggcggcaa cgacgcgtgg
     5341 gctgcgcgcg acgatgctca gcgcttcatc gagcgttacc gtggcgggaa gctgcgccca
     5401 ggccggcccg gcggcgaacg ccagcgcgaa cagctcaagc cgtctcatcg agctcctcgg
     5461 gagtagtgta cttctcggct gcgagcgcat agtagaccgc gggcaggacg cacagcgtga
     5521 tgaccagcgt gctcaccatg ccgccgacga tcaccagcgc gaacgggcgc tgcgtctcgc
     5581 tgccgatgcc ggtggaaagc gccatcggca gcaggccgaa cagcgcgagc agcgccgcca
     5641 tcagcaccgc gcgaagctt
//
LOCUS       DASPXW010000372         3785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1621323, whole genome shotgun sequence.
ACCESSION   DASPXW010000372 DASPXW010000000
VERSION     DASPXW010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1621323"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VEL04_08640"
     CDS             complement(<1..329)
                     /locus_tag="VEL04_08640"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HYT48475.1"
                     /translation="MEDRVAELRSRWTPESTAAAVAFLESRPTGYDLPTVEQGGVTYL
                     DLRGLRIEQTQIDKALLRNVNLRWALFRDVGFKNARFVNCNLSQVSFMECYLRRAQFE
                     RCDLVNSR"
     gene            complement(373..789)
                     /locus_tag="VEL04_08645"
     CDS             complement(373..789)
                     /locus_tag="VEL04_08645"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HYT48476.1"
                     /translation="MRQREAVFVDSGAWIALALSRDPLHVQAREQWDVLHGAAAKLHT
                     SVPVVIETFTFLERNANRDVALAWKDSIYKPGTVKVLPCDLRDLEQSWEYFRRADLHK
                     LSAVDATSFTIMKRARIRLAFSFDHHFPVVGFRLVT"
     gene            complement(789..998)
                     /locus_tag="VEL04_08650"
     CDS             complement(789..998)
                     /locus_tag="VEL04_08650"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HYT48477.1"
                     /translation="MEKTQVYLRKEELDALRKAAARSGRSVAALVRDAIRKVVLKPQV
                     AGPVAIWDGDPKRTSVDHDSVHDEP"
     gene            complement(1050..2231)
                     /locus_tag="VEL04_08655"
     CDS             complement(1050..2231)
                     /locus_tag="VEL04_08655"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYT48478.1"
                     /translation="MTANHRPPLEHFVSQEPFEPYAAEALTPEQERFYRASQWQIMWW
                     KFKRHRIAVIAGALLLAFYASILVSELLAPYNLHTRDTRHIYAPPQPLHLFHEGRFVG
                     PFVYGYHMRLNLVTLKREYTDDAATLHRLRFFCLGDEYQFWGLAGGSFHLVCPAEGGT
                     LYLLGTDRLGRDLLSRIIYGTRISLTVGLFGIVVSFLIGITLGGISGYYGGWIDTVIQ
                     RFIEMVRSFPELPLWMALSAALPVTWSPILVYFGITIILGLLDWPGLARAVRSKLLAL
                     REEDFATAAVLMGASPARVIRRHLLPSFASHLIASATLSVPAMILGETALSFLGLGLR
                     PPITSWGVLLNEAQNINVVALYPWLMLCVVPVFVVVLSFNFFGDGLRDAADPYSSAPR
                     T"
     gene            complement(2228..3253)
                     /locus_tag="VEL04_08660"
     CDS             complement(2228..3253)
                     /locus_tag="VEL04_08660"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYT48479.1"
                     /translation="MLRYTLHRLLIMIPTLLVISFITFMIIKLPPGDFLSNQIAELKS
                     QGDSAALEKVDFLRKQYGLDKPFIEQYAVWVGLWPTERGFSGLIQGDLGWSFEHDLPV
                     KEVVGDRLLLSFILNFSVILFTWAVSFPIGVYAATHQYSWGDHGLTLLGYVGLATPNF
                     LLALVLMYFANVGFGLSVGGIMAPEFLGEPWSWAKARSVLEHLWIPVIVIGTSGTAAM
                     IRRLRANLLDELQKQYVVTARAKGLPPFRLLVKYPLRMSLNPFIADIGNLLPSVISGS
                     AIVAVVLSLQTSGPMLLEALRSQDQYLAGSFLMFLSLLTVIGMFISDLLLAALDPRIR
                     LTGGAAK"
     gene            complement(3258..>3785)
                     /locus_tag="VEL04_08665"
     CDS             complement(3258..>3785)
                     /locus_tag="VEL04_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48480.1"
                     /translation="SEQSDVLELIRETWREVGIQLFSKPSQREAFRNRIFAGETVMSV
                     WSGIENGLPTPDTSPAELAPTSQLQLQWPKFGQYFETSGKTGEAPDIPSVQELAELYR
                     EWRRSISPEERRRIWNTMLAIHAQEQFVIGVVSGVPQPVIVRDTLMNVPEQAFYNWDP
                     GAFFGIYHPDTFWFK"
BASE COUNT          714 a         1183 c         1269 g          619 t
ORIGIN      
        1 cgcgaattga ccaggtcgca cctctcgaac tgggcgcggc gcaggtagca ctccatgaac
       61 gagacctggc ttaggttgca gttcacgaac ctggcgttct tgaagcccac gtcgcggaac
      121 agcgcccagc gcaggttgac gttgcgcagc aaggccttgt cgatctgcgt ctgctcgatg
      181 cgcagcccgc gcaggtcgag gtaggtgacg ccgccctgct ccaccgtcgg caggtcatag
      241 cccgtgggac gcgattcgag gaacgccacc gcggcggcgg tcgactccgg cgtccagcgc
      301 gatcgaagct cggctacgcg atcttccacg tttttcctta ttggatcact tcatcggccc
      361 gcaccagcag cgctacgtca ccagcctgaa tccaaccacg gggaagtggt gatcgaagct
      421 gaaggcgagc cgaatgcgcg cgcgtttcat gatagtgaag ctggttgcgt caacggcgga
      481 gagcttgtgc aggtccgcgc gccgaaagta ctcccacgac tgctcgagat cgcggagatc
      541 gcagggaaga actttgacgg tgccaggctt atagatcgag tctttccatg ccagcgccac
      601 gtcccgattg gcgttacgtt caagaaacgt gaatgtctcg atgacgacgg gaacggaggt
      661 gtgaagcttc gcggctgctc cgtgcagcac gtcccattgt tctcgcgctt gcacatgcaa
      721 ggggtctcgc gacagcgcaa gcgcgatcca tgccccgctg tctacgaaaa ccgcttcgcg
      781 ctgccgcatc agggctcgtc gtgaacgcta tcgtgatcga cggaagtccg cttgggatcg
      841 ccgtcccaga tggcgaccgg gcctgcaacc tgcggtttga gcacgacctt gcggatcgcg
      901 tcacggacga gtgccgcgac gcttcgcccc gaccgcgccg cggccttgcg cagggcgtcg
      961 agctcttcct tgcgcaggta aacctgggtc ttttccatgt atgtcacttt agttctatga
     1021 ttgacatacg tcaagctcgg gagttcctgt catgtccgcg gagcgctgct gtacgggtcg
     1081 gcggcgtcgc gcaggccatc gccgaagaag ttgaacgaga gcaccaccac gaacaccggt
     1141 accacgcaca gcatgagcca gggatagagc gcgaccacgt tgatgttctg cgcttcgttc
     1201 aggagcaccc cccagctcgt aatgggtggt cttaatccca atcccaaaaa agacagggcg
     1261 gtttcgccca gaatcatcgc cggcaccgaa agcgtcgccg aggcgatgag gtggctcgcg
     1321 aagctcggca ggaggtggcg gcggatgacg cgcgccgggc tcgcgcccat cagcaccgct
     1381 gcggtcgcaa aatcttcttc tcttaaagcc aaaagcttcg accgtaccgc gcgcgccagg
     1441 ccaggccagt cgagcagccc gaggatgatg gtgatgccga aatagaccag gatcgggctc
     1501 caggtcaccg gcagcgccgc cgagagcgcc atccacagcg gcagctcggg gaacgagcgc
     1561 accatctcga tgaagcgctg gatgacggtg tcgatccagc cgccgtagta gcccgagatg
     1621 ccgccgagcg tgatgccgat gaggaagctc accacgatgc cgaacaggcc cacggtgagc
     1681 gagatgcgcg tgccgtagat gatgcgcgag agcagatcgc gcccgaggcg gtcggtgccg
     1741 agcaggtaga gcgtgccgcc ttcggccggg cagacgagat ggaagctgcc gccggccagc
     1801 ccccagaact ggtactcgtc gcccaggcag aagaagcgca gccggtgcag cgtcgcggca
     1861 tcgtccgtgt actcgcgctt cagcgtgacg aggttgagcc gcatgtggta gccgtagacg
     1921 aacggcccga cgaagcgccc ctcgtgaaac aggtgcagcg gctgcggcgg cgcgtagata
     1981 tggcgcgtgt cgcgcgtgtg caggttgtaa ggcgcgagca gctcgctcac caggatcgag
     2041 gcgtagaacg caagcaggag cgcgcccgcg atcacggcga tgcggtggcg cttgaacttc
     2101 caccacatga tctgccactg cgaggcgcgg tagaagcgct cctgctcggg ggtgagcgcc
     2161 tcggcggcgt agggctcgaa cggctcctgc gagacgaagt gttcgagtgg tgggcggtga
     2221 ttcgccgtca cttcgcggcg ccgcccgtca agcggatgcg cgggtcgagc gccgcgagca
     2281 gcaggtcgga gatgaacatg ccgatcacgg tgagcagcga caggaacatg aggaaggagc
     2341 ccgccagata ctggtcctgg ctgcgcagcg cctcgagcag catcggcccc gaggtctgca
     2401 gcgacagcac cacggcgacg atcgccgagc cggagatcac gctcggcagc aggttgccga
     2461 tgtcggcgat gaacgggttg agcgacatgc gcagcgggta cttcaccagc agcctgaagg
     2521 gcggcaggcc cttggcgcgc gcggtcacca cgtactgctt ctgcagctcg tcgagcaggt
     2581 tggcgcgcag gcgccggatc atcgcggcgg tgcccgaggt gccgatcacg atcaccggga
     2641 tccacaggtg ctcgagcacc gagcgcgcct ttgcccagct ccacggctcg ccgaggaact
     2701 ccggcgccat gatgccgccg accgacaggc cgaacccgac attcgcgaag tacatgagca
     2761 ccagcgcgag caggaagttg ggcgtggcga ggccgacgta gccgagcagc gtcaggccat
     2821 ggtcgcccca gctgtactgg tgcgtggcgg cgtacacgcc gatcgggaac gacaccgccc
     2881 aggtgaacag gatcaccgag aaattcagga tgaacgagag cagcagccgg tcgccgacca
     2941 cctccttcac cggcagatcg tgctcgaacg accagccgag gtcgccctga atcaggccgg
     3001 agaagccgcg ctcggtcggc cacagcccga cccacaccgc gtactgctcg atgaacggct
     3061 tgtcgaggcc gtactgcttg cgcaggaaat cgaccttctc gagcgccgcg ctgtcgccct
     3121 ggctcttcag ctcggcgatc tggttggaga ggaaatcccc cggcggcagc ttgatgatca
     3181 tgaaggtgat gaagctgatc acgagcagcg tcgggatcat gatcagcagc cgatgcaggg
     3241 tgtagcgcag cacgctctta tttgaaccag aaagtatcgg ggtgatagat gccgaagaag
     3301 gcgcccgggt cccagttgta gaacgcctgc tcggggacgt tcatcagcgt gtcgcgcacg
     3361 atcaccggct gcggcacgcc ggacactacg ccgatcacga actgctcctg cgcatgaatc
     3421 gcgagcatgg tgttccagat gcgccggcgc tcctccgggc tgatgctgcg gcgccactcg
     3481 cggtagagct cggcgagctc ctgcaccgag gggatgtcgg gtgcttcgcc ggtcttgccg
     3541 ctggtctcga agtactggcc gaatttcggc cactgcagct gcagctggct ggtgggggcg
     3601 agctcggcgg ggctggtgtc gggcgtcggc aggccgttct cgatcccgct ccagaccgac
     3661 atcacggtct cgccggcgaa gatgcggttg cggaaagcct cgcgctgcga gggcttgctg
     3721 aagagctgga tgccgacctc gcgccaagtc tcgcggatca gctcgagcac gtcgctctgc
     3781 tcgga
//
LOCUS       DASPXW010000373         2096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_486076, whole genome shotgun sequence.
ACCESSION   DASPXW010000373 DASPXW010000000
VERSION     DASPXW010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486076"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="VEL04_08670"
     CDS             <1..770
                     /locus_tag="VEL04_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HYT48481.1"
                     /translation="GKETIGRGLWNSGLVKALSGGSLGVLNVAATCAGAGIIVGTVTL
                     TGLGLKFSSIVLQYAGGSLLLTAIYTSLIVWIVGLAVPVTASYIICAVIAAPALIQLG
                     VPDFAAHMFIFYYAVLSEVSPPTALSPFAAAAITGGDPYKTTLQCWKYTTPAFLVPFM
                     FVLDPSGTGLLLTGSFKTLGNADWGSIALVTLTAAAGIVALAGGLQGWLFKRTSLAER
                     LMLIVAGFLLVYPKTLFDVIGFALVALVLLMQWLKKN"
     gene            767..>2096
                     /locus_tag="VEL04_08675"
     CDS             767..>2096
                     /locus_tag="VEL04_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HYT48482.1"
                     /translation="MKALETDVLILGSGGAGLFAALHAHKANPGLSITVAVKGLLGKC
                     GCTRMVQGGYNVALAREDSVERHFMDTIEGGKWLPDQELAWKLVTLAVERIRELENEL
                     GCFFDRNPDGTVHQKAFAGQSFDRTVHKGDLTGIEIVNRLAEQVWVRKIGRLEEHRAV
                     ELIKNSSADAISGVLFIDMRSGEYVFVRAKAVLLATGGGPTMYKYHTPSGDKSCDGLA
                     MALRAGLRLRDMEMVQFHPTGLLAGTHTRMTGTVLEEGLRGSGGHLLNGARERFMPLY
                     DPLAERATRDIVSRAMFSEMRSGNTSPNGGLFISMRHLNQDLVKKQFRGMVERCADCG
                     FDLAGGLVEVVPTAHYMMGGLEFAPDCSTGLAGLFVAGEDAGGVHGANRLGGNGVANS
                     TVFGAVAGDSMAAWVRNEALRQPDPAAIDAAIAHCERPFEAGARGDLEPLR"
BASE COUNT          347 a          678 c          701 g          370 t
ORIGIN      
        1 ccggaaaaga aacaatcggc cgcggcctgt ggaactccgg gctggtgaaa gcgctgtccg
       61 gaggctcgct cggcgtgctg aacgtggccg ccacctgcgc cggcgccggc atcatcgtcg
      121 gcaccgtcac gctcaccggg ctcggactca agttcagctc catcgtgctg caatatgccg
      181 gcggctcgct cctgctcacg gcgatctata cctcgctcat cgtctggatc gtcggccttg
      241 ccgtgccggt gaccgcgagc tacatcatct gcgcggtgat cgccgcgccg gcgctgatcc
      301 agctcggcgt gccggacttc gccgcgcaca tgttcatctt ctactacgcg gtgctatccg
      361 aggtttcgcc gccgaccgcg ctctcgcctt tcgccgccgc ggcgatcacc ggcggcgatc
      421 cgtacaagac cacgctgcag tgctggaagt acaccacgcc cgcgttcctc gtgcccttca
      481 tgttcgtgct cgacccgagc ggcaccgggc tgctgctcac cggctccttc aagactctcg
      541 gcaatgccga ctggggctcg atagcgctcg tcaccctcac cgctgccgcg ggtatcgtcg
      601 cgctggcggg cggcctgcag ggatggcttt tcaagcgcac cagtctcgcc gagcgcttga
      661 tgctgatcgt cgccgggttc ctgctcgtct acccgaagac cctgttcgac gtgatcggct
      721 ttgcgctggt cgcgctggtc ctcctgatgc agtggttgaa gaaaaattga aggcgcttga
      781 gaccgacgtc ctgatcctcg gctcgggcgg cgcaggcctg ttcgctgcgt tgcacgcgca
      841 caaggccaat cccggtcttt ccatcacggt cgcggtgaag gggctgctcg gcaagtgcgg
      901 ctgcacgcgc atggtgcagg gcggctacaa cgtcgcgctc gccagggaag actcggtcga
      961 gcgccacttc atggatacga tcgagggtgg caaatggctg cccgaccagg agctcgcctg
     1021 gaagctggtg acgctcgcgg tcgaaaggat ccgggagctc gagaacgagc tcggctgttt
     1081 cttcgaccgc aatcccgacg gcacggtcca tcagaaggcg ttcgccgggc aaagcttcga
     1141 ccgcacggtg cacaagggcg acctcacggg catcgagatc gtcaaccgtc tcgccgagca
     1201 ggtgtgggtg cgcaagattg gacgcctcga ggagcaccgt gcagttgaat tgataaaaaa
     1261 ttcttccgct gacgcaattt cgggcgtgct gttcatcgac atgcgcagcg gcgaatacgt
     1321 gtttgtgcgc gccaaggcag tactcctcgc caccggcggc ggcccgacga tgtacaagta
     1381 ccacacgccc tcgggagaca agagctgcga cgggctcgcg atggcgctgc gcgccgggct
     1441 gcggctgcgc gacatggaga tggtgcagtt ccatcccacg gggctgctcg cgggcacgca
     1501 tacccgcatg acgggcacgg tgctcgagga gggattgcgc ggctccggcg gccacctgct
     1561 gaacggcgcc agagagcgct tcatgccgct gtacgatccg ctggcggagc gcgctacgcg
     1621 cgacatcgtg tcgcgcgcaa tgttctcgga gatgaggagc ggaaatacga gccccaacgg
     1681 cgggctattt atttccatga ggcatttgaa ccaggacctc gtaaaaaaac aattccgcgg
     1741 catggtcgag cgctgcgccg actgcggttt cgacctcgcg ggcgggctgg tcgaagtggt
     1801 gccgacagcg cattacatga tgggcggatt ggaattcgcg cccgactgct cgaccgggct
     1861 cgcgggcctg ttcgtcgccg gcgaggacgc gggcggcgtg cacggcgcga accggctcgg
     1921 cggcaacggc gtggcgaact cgaccgtatt cggcgccgtg gcgggcgaca gcatggcggc
     1981 gtgggtaagg aatgaggccc tgcggcaacc ggatcccgcc gcgatcgatg ccgcaatcgc
     2041 gcactgcgaa cggcctttcg aagccggcgc gagaggcgat ctcgagccgc tgcgcg
//
LOCUS       DASPXW010000374         4091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_235009, whole genome shotgun sequence.
ACCESSION   DASPXW010000374 DASPXW010000000
VERSION     DASPXW010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235009"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /gene="pxpB"
                     /locus_tag="VEL04_08680"
     CDS             complement(<1..647)
                     /gene="pxpB"
                     /locus_tag="VEL04_08680"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004266314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HYT48483.1"
                     /translation="MATPRPLAVIPLGDCAAYVEFSRTLDLEVNALVQRLAAAVHARG
                     EPWIRDVVPAFGGLALHFDPGFEGSVLEVALELVELCIKKGLPAAGELGREVEVPVCY
                     EREFAPDLDEVAKKTGLAPEEIARRHAAGEYRVLMIGFSPGHAYMGGLDAALAVPRRA
                     SPRAEVPAGSVAIANQQTVIYPYAISGGWSVIGRTPLAVFDAAREPPSLFGPGDRV"
     gene            complement(657..1250)
                     /gene="nthA"
                     /locus_tag="VEL04_08685"
     CDS             complement(657..1250)
                     /gene="nthA"
                     /locus_tag="VEL04_08685"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:NF014973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HYT48484.1"
                     /translation="MSDRGKYQPLSYFQLMEVTLRELLVEKGVLTEEQVAAEVQAMRE
                     RTPERGARVVAHAWTHPEYKKRLLENGTGACEELGLEIPALKLVVVENLPEVHNVIVC
                     TLCSCYPRVLLGIPPDWYKSRNYRSRMVREPRKVLAEFGLQIPGKTTIRVHDSTADMR
                     YMVLPMRPPGTDGWSEQQLARIVTRNSMIGVAVPKTT"
     gene            complement(1247..1492)
                     /locus_tag="VEL04_08690"
     CDS             complement(1247..1492)
                     /locus_tag="VEL04_08690"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:NF014289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3-like domain-containing protein"
                     /protein_id="HYT48485.1"
                     /translation="MKAYPLGHVRTPFYIRGHVGTIERFCGSFPNPEELAQMRDGLPA
                     QPLYRVRFTQKEVWPDYRGSAADVVEVEIFQHWLEKA"
     gene            complement(1527..1823)
                     /locus_tag="VEL04_08695"
     CDS             complement(1527..1823)
                     /locus_tag="VEL04_08695"
                     /inference="COORDINATES: protein motif:HMM:NF045268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48486.1"
                     /translation="MIQRGHHDMGGLPAGKVEPTEHDYAAWERRVDALSMLMGRKGVT
                     VDERRKNIEMLPPEAYDKMSYYERWVISLAQALIQRGIVTTAELARKMSEIDKR"
     gene            complement(1820..3520)
                     /locus_tag="VEL04_08700"
     CDS             complement(1820..3520)
                     /locus_tag="VEL04_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HYT48487.1"
                     /translation="MNKPVELQAVALTGAEAVVGMLRAHGVEVIFGLCGDTSLPLYDA
                     LARLGSIKHVLTRDERHAAYMADGYARVTGKVGVCEGPSGGGATYILPGLVEANESSI
                     PILAINTDVSVSSRGKFTLTELDQRSLMKPLTKWNAVLDRSADIPRVFRAAFEAMTSG
                     RPGAAHIALPFDVQNGPVDRGEVWADPTLGSYPSRRTSPDPFFVDLAAKLLRSAQHPV
                     FICGGGVVISGAENELSSLAEKLSAPVATTISGKGSIDERCALSVGVVGSNGGTPETR
                     AVVDAADVVIFAGCRAGSVTTERWRHPAPRRARVIHIDVDPAVPGTNYPVDVPLVGDA
                     QLCLASLNQALEGFERHGDLSFVDGKKRQKFEKFDALAASNESPIRPERMVLELGKSL
                     DPDAIVVADPGTPCPYLSAYYVVRGTGRRYFSNRAHGALGYALAASVGAHFGRPAVKT
                     VAVMGDGSFGMCAGELETLVRLKLPITLVVIANAAYGWIKAGQKTGYGERYFSVDFGV
                     TDHARVAEAFGVKSWRVSDPFQLSGVFRQAISAGGPTLVDVVCQPLHEAMAPVSEWIA
                     "
     gene            3540..>4091
                     /locus_tag="VEL04_08705"
     CDS             3540..>4091
                     /locus_tag="VEL04_08705"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYT48488.1"
                     /translation="MTIAERQKPAAGELYLDHVAHFVPDLDAAGRLAEALGFSVTPVS
                     AHRDQNGPAGTSNRCLMFEQGYVEILAPTLDTPNARRVRERMKSFVGVHLACFGTPDA
                     EAEHRRLAAHGFEPEPLVRLERNVDARTTVRFNVVHLPPAKMPEGRMQYVQQLTPEHL
                     WQPRHVNPFRLTGVYVVAEDPVQT"
BASE COUNT          712 a         1421 c         1286 g          672 t
ORIGIN      
        1 acccggtcac cggggccgaa caggctcggc ggctcgcgcg cggcatcgaa caccgcgagc
       61 ggggtgcggc cgatcacgct ccagcccccg gagatcgcgt acggatagat caccgtctgc
      121 tggttcgcga tcgccaccga gcccgccggc acctcggcgc gcgggctcgc gcgccgcggc
      181 accgcgagcg ccgcatcgag cccgcccatg tacgcgtgtc ccggcgaaaa gccgatcatc
      241 aggacgcggt actcgccggc ggcgtggcgg cgcgcgatct cctcgggcgc gagcccggtc
      301 ttcttcgcca cctcgtcgag gtcgggcgcg aactcgcgct cgtagcacac cggcacctcg
      361 acctcccggc cgagctcccc ggcggccggc agccctttct tgatgcacag ctcgaccagc
      421 tcgagggcca cctcgagcac gctgccctcg aagcccgggt cgaagtgcag cgccagcccg
      481 ccgaatgccg gcacgacgtc gcggatccag ggctcgccgc gcgcgtggac cgccgcggca
      541 agacgctgga ccagcgcatt cacttcgagg tcgagcgtcc tcgagaactc gacgtacgcc
      601 gcgcaatcgc cgagcggtat gacagcgagc ggcctcggcg tagccattaa ttcttcttaa
      661 gttgtcttcg gtacggcgac gccgatcatc gaattgcggg tgacgattct cgccagttgc
      721 tgctcgctcc acccgtcggt gcccggcggg cgcatcggca gcaccatgta gcgcatgtcc
      781 gcggtcgagt cgtgcacgcg aatggtcgtt tttcccggga tttgcaggcc aaactccgcc
      841 agcactttgc gcggctcgcg caccatgcgc gagcggtagt tgcgcgactt gtaccagtcg
      901 ggcgggatgc cgagcagcac gcgcggatag cacgagcaga gcgtgcacac gatgacgttg
      961 tgcacttcgg gcaggttctc caccaccacc agcttgagcg ccggaatctc gaggccgagc
     1021 tcctcgcagg ccccggttcc gttctcgagt aatctttttt tgtactcggg atgagtccaa
     1081 gcatgcgcga cgacgcgcgc accccgttcg ggggtgcgtt cgcgcatcgc ctgcacctcg
     1141 gccgcgacct gctcctcggt aaggacgcct ttctccacca gcagctcgcg cagcgtcact
     1201 tccatcagct ggaaatagct cagtggctgg tatttgccgc gatcactcat gctttctcga
     1261 gccaatgctg gaagatctcg acctcgacca cgtcagccgc gctgccgcga tagtccggcc
     1321 acacctcctt ctgcgtgaag cgcacgcgat acagcggctg cgcaggcagc ccgtcgcgca
     1381 tctgcgccag ctcctcggga ttgggaaagc tgccgcagaa acgctcgatg gtgccgacgt
     1441 ggccacgaat gtagaacggc gtgcgcacat ggccgagcgg gtaggctttc atgactcgca
     1501 cccgatcgcc aactcgaaaa agggattcaa cgtttatcaa tttcgctcat cttgcgggca
     1561 agctcagcgg tggtgacgat gccgcgctgg atgagcgcct gcgcaagcga aatgacccaa
     1621 cgctcgtagt agctcatctt gtcgtaagcc tccggcggca gcatctcgat gttcttgcgc
     1681 cgctcgtcga ccgtgacgcc cttgcggccc atcagcatgg acagcgcgtc cacccgccgt
     1741 tcccatgcgg cgtaatcatg ctcggtcggc tcgaccttgc cggccggcag cccgcccatg
     1801 tcatggtggc cccgctggat catgcgatcc actccgagac cggcgccatg gcctcgtgca
     1861 gaggctggca cacaacgtcc accaaggttg gaccgccggc cgaaattgcc tgacgaaata
     1921 ctcctgaaag ctgaaaagga tccgacaccc tccagctctt cacgccgaaa gcctcggcca
     1981 cccgggcgtg gtcggtaacg ccgaagtcga ccgagaaata gcgctcgcca tagcccgttt
     2041 tctgcccggc tttgatccag ccgtaggcgg cgttggcgat gaccaccagg gtgatgggca
     2101 gcttgaggcg caccagcgtc tcgagctcgc ccgcgcacat gccgaagctg ccgtcgccca
     2161 tcaccgccac ggtcttcacc gcaggccggc cgaaatgcgc gccgaccgac gccgcgagcg
     2221 catagccgag cgcgccgtga gcgcggttgg agaaatagcg gcggcccgtg ccgcgcacga
     2281 cgtagtacgc cgagaggtaa gggcaaggcg ttcccgggtc ggcgaccacg atggcatccg
     2341 gatccaggga ttttcctaat tcaagaacca tcctttcggg cctgatcggt gattcgttcg
     2401 atgccgcgag cgcgtcgaat ttctcgaatt tctgccgttt cttgccgtcg acgaaagaca
     2461 gatcgccgtg acgctcaaaa ccttccaggg cttgattgag ggaagcaaga cagagctgcg
     2521 cgtcgccgac gagcggtacg tcgaccgggt aattcgtccc cggaaccgca ggatcgacgt
     2581 cgatgtggat cacgcgcgcg cgacgcggcg cgggatggcg ccagcgctcg gtggtcaccg
     2641 agccggcgcg gcagccggcg aagatcacca cgtccgcggc gtcgaccacc gcgcgcgtct
     2701 ccggggtgcc gccgttcgag ccgaccactc cgaccgagag cgcgcagcgc tcgtcgatcg
     2761 agcccttgcc gctgatggtg gtggccaccg gcgcggacag cttttccgca agcgagctca
     2821 attcattttc cgctcccgag atcaccacgc cgccgccgca gatgaatacc ggatgctgcg
     2881 cgctgcgcag cagcttcgcc gcgaggtcga cgaaaaaagg atcgggagaa gttcttcgag
     2941 aaggatagct gccgagcgtc ggatcggccc acacctcgcc cctgtcgacc gggccgttct
     3001 gcacatcgaa gggcagcgcg atgtgcgccg cccccggccg gccgctggtc atggcctcga
     3061 acgcggcgcg gaacacgcgc ggaatgtcgg ctgagcgatc gagcaccgcg ttccacttgg
     3121 tcagcggttt catcaaagac cgctggtcga gctccgtcag cgtgaatttc ccccgggaag
     3181 aaaccgagac atcggtattg atggcaagga tcgggatcga cgactcgttc gcctcgacca
     3241 gcccgggcag gatgtaggtt gcgccgccgc ccgagggccc ttcgcatacg ccgaccttgc
     3301 cggtcacgcg cgcgtagccg tcggccatgt acgccgcgtg cctttcatca cgggtcaata
     3361 cgtgcttgat cgaacccagg cgggcaagcg catcgtagag cggcaggctg gtgtcgccgc
     3421 acaagccgaa gatcacctcg acgccgtgcg cgcgcagcat cccgaccacc gcttcggccc
     3481 ccgtcagcgc gacggcctgc agctcgaccg gcttgttcat gcggttatat tagcgcggca
     3541 tgactatcgc agagcgccag aaaccggccg ccggagagct ctacctcgat cacgtcgcgc
     3601 acttcgttcc agatctcgac gccgcggggc ggctcgccga agcgctcggc tttagcgtca
     3661 cgccggtctc ggcgcaccgc gaccagaacg ggcccgccgg cacctccaac cgctgcctca
     3721 tgttcgagca gggctatgtc gaaatcctcg cgccgacgct cgacacgccg aatgcccgcc
     3781 gcgtccgcga gcggatgaaa tcattcgtcg gggttcacct tgcatgcttc ggcacgccag
     3841 acgcggaagc ggagcaccgc aggctggccg cccacggctt cgagcccgag ccgctcgtca
     3901 ggctggaaag aaatgtcgac gcgcggacca ccgtgcgctt caacgtcgtc cacctgcctc
     3961 cggcgaaaat gccggaagga cgcatgcagt acgtgcagca gctcacgccc gagcacctgt
     4021 ggcagccgcg ccacgtgaat ccgtttcgcc tcacgggggt gtacgtcgtc gccgaggacc
     4081 ccgtgcagac g
//
LOCUS       DASPXW010000375         3323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_235306, whole genome shotgun sequence.
ACCESSION   DASPXW010000375 DASPXW010000000
VERSION     DASPXW010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235306"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1109
                     /locus_tag="VEL04_08710"
     CDS             <1..1109
                     /locus_tag="VEL04_08710"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HYT48489.1"
                     /translation="LGERIPGVEVHAQILEQMFDGTYLRRPTAAAWIEAALLAGAGVV
                     FVMFVPAVRPAISPLLLAGVLAALTAIGMLGFRSGLLLNVAAPALGAALVLGALLAST
                     LAEADRQRRLLREAQARVAGELEAARRIQMGLLPMPRALFAGEPRFVLDALLEPARSV
                     GGDFYDCFMLDQDRVFFVVGDVSGKGLGASLFMALSKSLLKSIALRAEDEPAAIVERA
                     NAEISRDNPESLFVTAFAGVLDARSGNLAFCNAGHEPPAVCSPGEAPKRVMHSGGPPL
                     CVMRDYAYSSDQVNLAQGGWLCVVTDGVTEAMNARGELYGSARLLSLLRGLESAAPDA
                     VIAAVRDDVRRFAGGAEQSDDVTLLCVRWTGNAS"
     gene            complement(1131..1724)
                     /locus_tag="VEL04_08715"
     CDS             complement(1131..1724)
                     /locus_tag="VEL04_08715"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HYT48490.1"
                     /translation="MAVLAIAALLALAIYVSTPATVDTVVVLPSPDGHTGTVVVQRGA
                     ERQVLDQPYAASRTAPGEPAEVSRLSAAEVNQSFGQVLEALPSRPIAFLLYFVTGSDE
                     LTDESKLEVQKVRAELKGRKVPDVLVIGHTDTVGDAEWNDRLSAQRAERVKAILVEAG
                     IAAARIQTAGRGERELLVRTADNVDEPRNRRVEINVR"
     gene            complement(1728..2165)
                     /locus_tag="VEL04_08720"
     CDS             complement(1728..2165)
                     /locus_tag="VEL04_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HYT48491.1"
                     /translation="MRWTIAAFLLGFTLTCLADDAGQIKTASGSVYLERDGKRLPVQV
                     GMPVRQSDILVSGADGAAGVTFTDNSLLSVGPNSVLAIERYAFDSTTHSGQFDASLRK
                     GTLAVISGKMVKQSPDAMRVRTPSSIMGVRGTEFVVRVEEPGN"
     gene            2260..2655
                     /locus_tag="VEL04_08725"
     CDS             2260..2655
                     /locus_tag="VEL04_08725"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYT48492.1"
                     /translation="MFPARLAQLRRLGGFLEGFCAARGIGREHCLRLNLVLEELFVNT
                     VRHGHRGDCDAPVWVSVAAEPAAVRVTYEDTAPPFNPYARLLAKSPDDTHEMRRIGGL
                     GVLLTRELAATRDYAYLFGRNRIRLTLKR"
     gene            complement(2644..3165)
                     /locus_tag="VEL04_08730"
     CDS             complement(2644..3165)
                     /locus_tag="VEL04_08730"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000334 - 3-hydroxyanthranilate
                     3,4-dioxygenase activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HYT48493.1"
                     /translation="MALAAFSFSQWIDEHARLLKPPVGNQLVFPAAEDLIVQVVGGPN
                     KRTDYHDDPYEEFFYQLRGNMVLNVIDGGRPGQIPVKEGEIVLLPAHFRHSPQRPEPG
                     SVGLVVERVRPLDVNDGFEWYCPKCWVLVHRVEVNVQNIVRDLPPLFEAFYAGKRQCP
                     RCGYIHPGKGLAL"
BASE COUNT          522 a         1113 c         1121 g          567 t
ORIGIN      
        1 cgctcggcga gcgcattccg ggagtcgagg tgcacgcaca gatcctcgag cagatgttcg
       61 acggcaccta cctgcgccgg ccgaccgcag ccgcctggat cgaggcggcg ctgctggccg
      121 gcgcaggggt cgtcttcgtg atgttcgtgc ccgcggtgcg gcccgcgatc tcgccgctcc
      181 tgctcgcggg cgtgcttgcc gcgctcaccg ccatcggcat gctcggtttc cgcagcgggc
      241 tgctgctgaa tgtcgccgca ccggccctcg gcgcggcgct ggtgctgggc gcgctgctag
      301 cctcgacgct cgccgaggcc gaccggcagc gccggctgct gcgcgaagca caggcgcgcg
      361 tcgcgggcga gctcgaggct gcgcgccgca tccagatggg gttgctgccc atgccgcggg
      421 cgctgttcgc gggcgagccg cgcttcgtgc tcgatgcgct gctcgagccg gcgcgcagcg
      481 tcggcggcga cttctacgac tgcttcatgc tcgaccagga ccgcgtgttt ttcgtggtgg
      541 gcgacgtctc gggtaaggga ctgggggcga gcctgttcat ggcgctgtcg aaatcgcttc
      601 tgaagagcat cgcgctgcgc gcggaggacg agcccgcggc catcgtcgag cgcgccaacg
      661 ccgagatcag ccgcgacaac cccgaatcgc tcttcgtcac tgcctttgcc ggcgtgctcg
      721 acgcccgcag cggcaacctc gccttctgca acgccggcca tgagccgccc gcagtgtgca
      781 gccccggcga ggcgcctaaa cgggtaatgc attcgggcgg tccgccgctt tgcgtgatgc
      841 gtgactatgc ctactcgagc gaccaggtca acctggcgca gggcggatgg ctgtgcgtgg
      901 tcaccgacgg ggtcaccgag gcgatgaatg cccgcggcga gctctacggc agcgcgcgcc
      961 tgctctcgct gctgcgcggc ctggaatccg ccgcgcctga cgcggtcatc gccgcggtgc
     1021 gcgacgacgt gcgtcgtttt gccggcggcg ccgagcagtc ggatgatgta acgctgcttt
     1081 gcgtgcgctg gaccgggaac gccagctagc cgttgcttgc cacgcggctg ctagcgaacg
     1141 tttatttcca cccgcctgtt gcgcggctca tcgacgttgt cggcggtgcg aaccagcagc
     1201 tcgcgctcgc cgcgcccggc agtctggatg cgcgcggcag caattccggc ctcgaccagg
     1261 atcgccttca ctcgctcggc gcgctgcgcc gagagccgat cgttccactc ggcgtcgccg
     1321 accgtatcgg tgtggccgat gacgagcaca tcgggcacct tgcggccttt cagctccgcg
     1381 cgcactttct gcacctcgag cttcgattcg tcggtgagct cgtcgctgcc ggtgacgaag
     1441 tacagcagga acgcgatcgg cctggaggga agcgcttcga gcacctgccc gaacgactgg
     1501 ttcacctcgg cggcggacag gcgcgagact tccgcgggtt cgcctggcgc ggtgcggctc
     1561 gcggcatacg gctggtcgag cacctggcgc tcggcgccgc gctgcaccac caccgtgccg
     1621 gtatggccgt caggcgatgg cagcaccacc acggtgtcga ccgtcgctgg cgtcgagaca
     1681 tagatcgcga gcgcgagcag cgcggcgatg gcgagcaccg ccatcggtca gtttccgggt
     1741 tcctcgacgc gcaccacgaa ctcggtgccg cgcacgccca tgatcgatga cggggtgcgc
     1801 acgcgcatgg cgtcgggcga ctgcttgacc atcttgcccg agatcaccgc gagggtgccc
     1861 ttgcgcagcg aggcgtcgaa ctggccgcta tgcgtcgtcg agtcgaaggc gtagcgctcg
     1921 atcgccagca cgctgttcgg tccgacggag agcaggctgt tgtcggtgaa agtcaccccg
     1981 gccgcaccgt cggcgccgct gaccaggata tcggactggc gcaccggcat gccgacctgg
     2041 accggcaggc gtttgccgtc gcgctccagg tataccgagc cgctggcggt cttgatctgg
     2101 ccggcgtcgt ccgccaggca agtgagggtg aagccgagca ggaatgccgc tatcgtccaa
     2161 cgcatggtgc cctcccaggg caaatctgag acagattcta taatgcgccg gggctcatta
     2221 cggacaaagt gacacaaagt gacaaatgtc gtatcggcga tgtttccggc taggctggcg
     2281 cagttgcggc gcctgggcgg gttcctcgaa ggcttctgcg ccgcgcgcgg catcgggcgc
     2341 gagcactgcc tgcggctcaa tctggtgctt gaggagctat tcgtcaacac cgtccggcat
     2401 gggcaccgcg gcgattgcga cgccccggtc tgggtgagcg tcgcagccga gcccgcggca
     2461 gtgcgcgtca cctatgagga caccgcgccg ccgttcaatc cctacgcccg gctgctggcg
     2521 aaatctcccg atgacacgca cgagatgcgc aggatcggcg ggctgggcgt gctgctcacc
     2581 cgggagctcg ccgcgacgcg cgattacgcc tatctcttcg ggcgcaaccg catccgcctg
     2641 acgctaaagc gctagcccct tgccggggtg gatgtagccg cagcggggac actgtcgctt
     2701 gccggcatag aacgcttcga acagcggcgg cagatccctg acaatgtttt gcacgttgac
     2761 ttcgacccga tgaaccagga cccagcattt cgggcagtac cactcgaagc cgtcgttgac
     2821 gtcgagcggc cgcacccgct ccacgaccag gccgaccgag cccggctcgg ggcgctgcgg
     2881 cgaatgcctg aagtgggcgg gaagcagcac gatttcgccc tccttcaccg gtatctgacc
     2941 cggtcggccg ccatcgatca cattcagcac catattgccg cgtagctggt agaagaactc
     3001 ttcgtacgga tcgtcgtggt aatcggtgcg cttgttaggg ccgccgacca cctgaacgat
     3061 caggtcttcg gcggcgggaa ataccagctg attgccgacc ggcggcttga ggagacgggc
     3121 gtgctcgtca atccactgcg aaaaactgaa tgcggcgagc gccatggccc atagtatatt
     3181 gacgcgtcgt gcggagcgct cagcaaatca tctgccgcaa atgcggcgtc atgtcgaacg
     3241 ttcaaaccat cgagaaaagc aacgacagcc tctacttcgt gcgctgccag cagtgccagg
     3301 caaagaacca gatcgtgcag acc
//
LOCUS       DASPXW010000376         2578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_615684, whole genome shotgun sequence.
ACCESSION   DASPXW010000376 DASPXW010000000
VERSION     DASPXW010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615684"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /gene="rnpB"
                     /locus_tag="VEL04_08735"
     ncRNA           complement(<1..283)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VEL04_08735"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            344..856
                     /locus_tag="VEL04_08740"
     CDS             344..856
                     /locus_tag="VEL04_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HYT48494.1"
                     /translation="MRRRLYFLLPDVESAKRTANDLLLARIEDRHMRFLAKRGTNLGE
                     LHEASFLFKTDLVHGAGIGLMLGALGGLVLGAIIVTYPPEGTKPQLVAVLIAAIVGAI
                     LGAWMASMAAAAVPNSRLKQFQDDIARGKILLMVDVPYGRIEQIRGIIVGRHPEAVSG
                     GQETRFPAFP"
     gene            complement(826..1548)
                     /locus_tag="VEL04_08745"
     CDS             complement(826..1548)
                     /locus_tag="VEL04_08745"
                     /inference="COORDINATES: protein motif:HMM:NF022673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3025 domain-containing protein"
                     /protein_id="HYT48495.1"
                     /translation="MRPWLRAGRSLDGLNALAEAGGIRTQAGKPVRFVAPAKTKHLNY
                     ELRVCESGCVATRAGNLHDWFNALAWLAFPRTKARINAMHAAQIPRERGRRGRLRDLL
                     TLFDEGGAIVRCDDDGLIAMLRQFHWRELFWEHRSGVLSSMRVVVFGHAVLEMALQPW
                     PGITCKAIFVPPEGDADAQAAAWLARLPLDATPQELAALPVYGYPGWLPGNDCAEFYE
                     DVRYFRPFRGKPPQGKAGKRVS"
     gene            complement(1569..>2578)
                     /gene="pyrC"
                     /locus_tag="VEL04_08750"
     CDS             complement(1569..>2578)
                     /gene="pyrC"
                     /locus_tag="VEL04_08750"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017333371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HYT48496.1"
                     /translation="PDDWHAHLRDGEPMAAVVAATARQFARAIVMPNLKPPVRTTEDA
                     LAYRDRILAALPAGAAFEPLMTLYLTDDTPPEEISRAKLSGRVHGVKLYPAGATTHSE
                     AGVTRLSRCFHALERMQAVGLPLLVHGETTEPAIDVFDREMVFIEEVLGPTLERFPGL
                     KIVLEHVTTREAVQFVEVTGPNVAATITAHHLLLNRNALFLGGVRPHHYCLPVLKREE
                     DREALVGAAVSGNPKFFLGTDSAPHARAAKEAACGCAGIYTAHAAIELYAIAFEEAGA
                     LDKLEGFASEFGARFYGLPVNGERITLLREDWKVPERLAFGGDELVPLRAGESIPWKL
                     A"
BASE COUNT          400 a          902 c          856 g          420 t
ORIGIN      
        1 ggcgggccgg cagccattcc tctgggccgc gggttacccc gcggctcaag ccacctaccc
       61 gcaagcatcg gccgagccgg cctcagtcgc ctgcctattt ggtgttgctc cggatggagg
      121 ttgccgcgtt tcacccctca gcttgcgccg aggactcgtc tctgtggccc tattcgtcac
      181 gtcgccgtgc cagggagtta ccctgcatcc cgctctgcgg agcccggact ttcctctatg
      241 ccgcctggaa ctacgaccgc acagcggctg cctggccgac tcccgttgca attttacccc
      301 cgcaaggtag agtccaagca ggaccaacac caggagccgg agcatgcgaa ggcgcctcta
      361 tttcctcctg ccagacgtcg aaagcgccaa gcgaaccgcg aacgacctgc tgcttgcgcg
      421 catcgaggac cggcacatgc gttttctcgc caagcgcggc accaacctcg gcgagctcca
      481 cgaggcgagt ttcctgttca agaccgacct ggtgcatggc gcgggcatcg gcctcatgct
      541 gggcgccctc ggcggcctgg tcctgggcgc gatcatcgtc acctatccgc cggaaggcac
      601 caagccgcag ctggtcgcgg tgctgatcgc cgccatcgtg ggcgccatcc tcggagcgtg
      661 gatggcgagc atggcggccg ccgcggttcc caattcgcgc ctcaagcagt ttcaggacga
      721 tatcgcgcgc ggcaagatcc tgctgatggt ggacgttccg tacggtcgca tcgagcagat
      781 ccgcggcatc atcgtggggc gccatcccga agccgtttcg ggcggtcagg agacccgctt
      841 ccccgctttc ccttgagggg gctttcccct gaacggacgg aaatagcgca cgtcttcgta
      901 gaactcggcg caatcgtttc ccggaagcca ccccggataa ccgtataccg gcagggcagc
      961 gagctcctgc ggcgttgcat cgagcggcag ccgcgccagc caggctgcgg cctgcgcgtc
     1021 cgcgtcgccc tcgggaggta cgaagatcgc cttgcaagtg atccccggcc agggctggag
     1081 cgccatctcg agcactgcgt ggccaaaaac caccacccgc atcgagctca gtacgccgct
     1141 gcggtgctcc cagaacagct ctctccagtg gaattggcgc agcatcgcta tcagcccgtc
     1201 gtcgtcgcat cgcacgatcg cgccgccctc gtcgaacagc gtcagcagat cgcgcagccg
     1261 gccgcggcgg ccgcgctcgc gcggaatctg tgccgcgtgc atggcattga tcctcgcctt
     1321 ggtgcgcgga aacgccagcc aggcgagcgc gttgaaccag tcgtgcaggt tgccggcgcg
     1381 ggtggcgacg cagcccgatt cgcacacccg cagttcgtaa ttcaagtgtt tggtttttgc
     1441 aggcgccacg aagcgcaccg gtttgccggc ttgcgtgcga atgccgcccg cctcggctag
     1501 cgcgttgagg ccgtcgagcg agcggccggc gcgcagccag ggccgcacgc cgtcgaacgc
     1561 cggatgcgct aggcgagctt ccaggggatc gattcgcctg cccgcagagg caccagctcg
     1621 tcgccgccga aagcgagacg ctccgggacc ttccagtcct cgcgcagcag cgtgatgcgc
     1681 tcgccgttca ccggcaggcc atagaaccgc gccccgaact cggatgcgaa gccctcgagc
     1741 ttgtcgagcg cgcccgcttc ctcgaaggcg atcgcataga gctcgatcgc cgcgtgggcg
     1801 gtgtagatgc cggcgcagcc gcaggccgcc tcctttgccg cgcgcgcgtg cggggcgctg
     1861 tcggtaccga ggaagaactt gggattgccc gacaccgcgg ccccgaccag cgcctcgcgg
     1921 tcctcctcgc gcttgagcac cggcaggcaa tagtggtgcg gccggacgcc gccgaggaac
     1981 agggcattgc ggttcaagag cagatggtgc gcggtgatcg tcgcggcgac gttcgggccg
     2041 gtgacctcga cgaactgcac cgcctcgcgc gtggtgacgt gctcgagcac gattttcagg
     2101 ccgggaaagc gctcgagcgt cggtccgagc acttcctcga tgaacaccat ctcgcggtcg
     2161 aagacgtcga tcgccggctc ggtcgtctcg ccgtgcacca gcagcggcag gccgacggcc
     2221 tgcattcttt caagcgcgtg aaagcagcgc gacagccgcg tcacgccggc ctcggagtgc
     2281 gtcgtcgcgc ccgccgggta gagcttgacg ccgtgcacgc gccccgacag cttggcgcgc
     2341 gagatttcct cgggcggcgt gtcgtcggtc aggtagaggg tcatcagcgg ctcgaacgcc
     2401 gcgcctgccg ggagcgcggc gagaatgcgg tcgcggtacg cgagcgcatc ctcggtagtg
     2461 cgcaccggcg gcttcaggtt cggcatcacg atcgcgcgcg cgaactggcg cgcggtcgcg
     2521 gcgacgaccg cggccatcgg ctcgccgtcg cgcaagtgcg cgtgccagtc gtccggcc
//
LOCUS       DASPXW010000377         2346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1874346, whole genome shotgun sequence.
ACCESSION   DASPXW010000377 DASPXW010000000
VERSION     DASPXW010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1874346"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..909)
                     /locus_tag="VEL04_08755"
     CDS             complement(34..909)
                     /locus_tag="VEL04_08755"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HYT48497.1"
                     /translation="MNQPLQEGSQLENYRILRVLAAGGFSFVYLAHDENGAPVAIKEY
                     LPSTLALRGDGDARPRIPDAELARFRYGMKCFFEEGRSLAHLAHPNVVRVLNFFRANE
                     TVYLVMRYERGRSLQEHIQSHRGTLEELWVRTTFAQLLDGLREVHSNKLLHLDIKPAN
                     VYLRNDGTPLLIDFGAARQAFSAEGLNLPPTYTPGFAAPEQYARRELLGPWTDIYSVG
                     ATLYACFAAAAPQSAERRMEQDLVVPARQAFAGKCSVEVLDTVDWCLRLNHLERPQSV
                     FALQKALLGEKPPER"
     gene            934..1812
                     /locus_tag="VEL04_08760"
     CDS             934..1812
                     /locus_tag="VEL04_08760"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HYT48498.1"
                     /translation="MIHSMTGYAAASVESPRGALAIELRSVNARFLDVQMRVAEELRA
                     LEPMLRERIAARIARGKVDCRVFFTEGSAPAGSQRLSAAALARLKTLADEARQAFPDA
                     AGMRIADVLRWPGVLAEAPADEGATRAAAEGLCRRALDELLAARAREGAKLAQVVSER
                     VAAMRRRLEEVAPLVPQSLAAYQAKLAERLREAIGSALGSADDDRVRAELAVFAAKVD
                     VDEELERLRAHLAEVERTLQGGGAAGKRLDFIAQELNREANTLASKAASQAISDCALE
                     LKLLIEQMREQVQNIE"
     gene            1826..>2346
                     /gene="gmk"
                     /locus_tag="VEL04_08765"
     CDS             1826..>2346
                     /gene="gmk"
                     /locus_tag="VEL04_08765"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HYT48499.1"
                     /translation="MRKGKLFVITAPSGAGKTSLIEAVMREDPSLKISVSYTTRPPRP
                     GEKNGVDYHFIDDATFLAMQNRGEFVESAEVHGYRYGTSKNVILDAVTRGEDLLLEID
                     WQGAQQVRKLYPDCVGIFILPPSIEELERRMRARGQDTDAVIRRRVENAHGELLHAGE
                     FKYAIINKDFDTAR"
BASE COUNT          402 a          769 c          817 g          358 t
ORIGIN      
        1 accctttaga tggccgagca gcggtgcggc gacctagcgc tcgggcggtt tttctcccag
       61 cagcgctttc tgcagcgcaa acacgctctg cggccgctcg agatggttga ggcgcaggca
      121 ccagtcgacc gtgtcgagga cctcgaccga gcacttgccg gcgaaggcct ggcgcgccgg
      181 caccaccagg tcctgttcca tgcgccgctc ggcgctctgc ggcgccgcgg cggcgaaaca
      241 ggcgtacagg gtggcaccga ccgagtagat gtcggtccac gggccgagca gctcgcgcct
      301 cgcgtactgc tccggagcgg cgaagcccgg cgtgtaggtc ggcggcaggt tcaggccctc
      361 ggcggagaac gcctgtcgcg cggcgccgaa atcgatgagc agcggcgtgc cgtcgttgcg
      421 caggtagacg ttcgccggct tgatgtcgag atgcagcagc ttgttcgaat gcacctcgcg
      481 cagcccgtcc agcagctggg cgaaggtggt gcgcacccac aattcctcga gcgtgccgcg
      541 gtggctctgg atgtgctcct gcagcgagcg cccccgctcg tagcgcatca ccagatacac
      601 ggtctcgttg gcgcggaaga aattcagcac gcgcaccacg ttcggatgcg cgagatgcgc
      661 gagcgagcgt ccttcctcga agaagcactt catgccgtag cggaagcgcg ccagctccgc
      721 atcgggaatg cgcgggcgcg catcgccgtc gccgcgcagc gcgagcgtgg agggcaggta
      781 ctccttgatt gcgaccggcg cgccgttctc gtcatgcgcg aggtacacga acgagaagcc
      841 gcccgcggcg agcacgcgca ggatgcggta gttctcgagc tggctgcctt cctgcagcgg
      901 ctggttcaat tgcacagcag gttatgataa cgaatgatcc acagcatgac cggctatgct
      961 gcggcgtcgg tcgaatcgcc gcgcggcgcg ctcgcgatcg agctgcgctc ggtgaacgcc
     1021 cgcttcctgg acgtgcagat gcgcgtcgcc gaagagctgc gcgcgctcga gccgatgctg
     1081 cgcgagagaa tcgccgcgcg catcgcgcgc ggcaaggtgg attgccgcgt tttcttcacc
     1141 gaaggcagcg ctccggccgg ctcgcagcgc ctcagcgccg cggcgctcgc ccgcctgaag
     1201 acgctcgccg atgaagcaag gcaggcgttt cccgatgccg ccggcatgcg catcgccgac
     1261 gtgctgcgct ggccgggcgt gctggccgag gcgccggccg acgaaggcgc gacgcgcgcg
     1321 gccgcagaag ggctctgccg gcgcgcgctc gacgagctgc tcgccgcgcg cgcccgcgag
     1381 ggcgcgaagc ttgcccaggt ggtgagtgag cgggtggcgg cgatgcgccg ccggctcgag
     1441 gaggtcgcgc cgctggtgcc gcaatccctc gccgcttacc aggcgaagct cgccgagcga
     1501 cttagggagg cgatcggctc ggcgctcggc tccgccgacg atgaccgcgt gcgcgccgag
     1561 ctggcggtat ttgccgcgaa agtcgacgtc gacgaagagc tcgagcggct gcgcgcgcat
     1621 ctcgccgaag tcgagcgcac gctgcaaggt ggcggggcgg ccggcaagcg cctggatttc
     1681 atcgcgcagg aactgaaccg cgaggcgaat acgctggcct ccaaggcggc gagccaggcg
     1741 atctcggatt gcgcgctgga gctgaagctc ctcatcgagc agatgcgcga gcaggtgcag
     1801 aacatcgaat gagaagcccg gagtaatgcg caaaggcaag ctcttcgtca tcactgcgcc
     1861 ttccggagca ggcaagactt ccctcatcga ggcggtgatg cgcgaggatc cgagcctgaa
     1921 aatctcggtc tcgtatacga cccggccgcc caggcccggc gaaaagaacg gcgtcgacta
     1981 ccacttcatc gacgacgcga cgtttctcgc catgcagaac cgcggcgagt tcgtggagag
     2041 cgccgaagtt cacggctacc gctacggcac gtcgaaaaac gtgatcctgg atgcggtgac
     2101 gcgcggcgag gacctgcttc tcgagatcga ctggcagggc gcccagcagg tgcgcaagct
     2161 ttatcccgat tgcgtcggca tcttcatcct gccgccttcg atcgaggagc tcgagcggcg
     2221 catgcgcgcg cgcggccagg atacggacgc cgtcatccgg cgccgggtgg aaaatgccca
     2281 cggcgaactg ctgcacgccg gagagttcaa atacgctata attaacaaag acttcgatac
     2341 tgcccg
//
LOCUS       DASPXW010000378         2908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1498465, whole genome shotgun sequence.
ACCESSION   DASPXW010000378 DASPXW010000000
VERSION     DASPXW010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1498465"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="VEL04_08770"
     CDS             complement(<1..896)
                     /locus_tag="VEL04_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48500.1"
                     /translation="MPKRLPGSREEDSWLSDSQLSGLARADEADELRSPIPTQVVSNG
                     EYLPFPQTPQQRQVEARIGEIAGEASRRLGMSRRRFLASSGGMAAAFIAMNEVFGRFF
                     DVDPLELMGSAHAQGAVPRDLFVFDDQLHMIRESFNGPLALRAMSQGAGAAASAAGFD
                     KNPFNTEGLPDELGRPWASWNPSLGQTPIVGANFHLVKFVKDVYLDSQVAVAILSNAP
                     LGLFEPPGGDKPRIPRSAGESLSAMNLTGYQTAAVRDWVNQIAGSTRLLAHGQIFPGK
                     NNLGFMQQQIDQFHPDSWKGYN"
     gene            1009..1503
                     /locus_tag="VEL04_08775"
     CDS             1009..1503
                     /locus_tag="VEL04_08775"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HYT48501.1"
                     /translation="MNKFAVCLLAGVLPFALAACASAPPSQPGRAYSAVSETVAGDAA
                     DQALKMLGRPYRAGGSTPAAGFDCSGLVQFSFRQAGLALPHNTDKQRSVSRPVRVADL
                     RRGDLLFFDQEGKKSSHVGIYVGAGKFVHAPSSGKHVRSDRLDSPYWKKHLSGARRLS
                     VFDA"
     gene            1571..1861
                     /locus_tag="VEL04_08780"
     CDS             1571..1861
                     /locus_tag="VEL04_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HYT48502.1"
                     /translation="MAKAYWITCYRSIKNPDALAAYAKLAGPAIQAAGGRFVVRGMPA
                     KTYEAGLDQRTVVTEWESVAKAIAAHDSPGYQEALRALGGAAERDVRIVEGA"
     gene            1865..2452
                     /locus_tag="VEL04_08785"
     CDS             1865..2452
                     /locus_tag="VEL04_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48503.1"
                     /translation="MAAVQQMVDPHDVVMTGENSFIRLSDDGGKSLVERVSHWRVLWS
                     PAGQGHALFIESSLLKTISVYADNAGLVRYLQGTIETLLHKPFADESLPVVDARFERS
                     GNSLSTVEERVVSRKDEIILAWWDLIAPFVLTMPPGALKRPLGVYSTFIPAKSAQLSV
                     NGQAATAKVFPQERFGRPSSSCCLAWSESWTRPRG"
     gene            2467..2676
                     /locus_tag="VEL04_08790"
     CDS             2467..2676
                     /locus_tag="VEL04_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48504.1"
                     /translation="MKLKLVSLAVAVVAVAWLGMWVSDTGLLVYSAEARVPLARDCRY
                     LIGVTVLKRIEPLAQRCEVLRTVGR"
BASE COUNT          497 a          961 c          946 g          504 t
ORIGIN      
        1 atgttgtagc ccttccacga gtcgggatgg aactggtcga tctgctgctg catgaaccct
       61 aaattgtttt ttccaggaaa aatttgtcca tgggcaagca ggcgggtgga acccgcgatc
      121 tggttcaccc agtccctgac cgctgcggtc tggtagcccg tcaggttcat cgcgctgagc
      181 gactcgccgg cgcttctcgg gatgcgcggc ttgtcgccgc ccggcggttc gaagagtccc
      241 agcggcgcat tgctgaggat cgcgaccgcc acctggctgt cgaggtacac gtccttgacg
      301 aacttcacca ggtggaagtt cgcgccgaca atcggcgtct ggccgagcga cggattccaa
      361 gaggcccacg gacgcccgag ctcgtccggc aggccctcgg tgttgaacgg gttcttgtcg
      421 aaaccggcgg cggaagccgc cgcaccggcg ccctgcgaca tcgcgcgcag cgccagcggg
      481 ccgttgaacg attcgcggat catgtgcagc tggtcgtcga acacaaaaag gtcgcgcggt
      541 accgcgccct gggcgtgcgc cgatcccatc agctcgagcg gatcgacgtc gaagaacctg
      601 ccgaacacct cgttcatcgc gatgaacgca gctgccatgc cgcccgaaga ggcgagaaag
      661 cgccgccggc tcatgccgag cctgcggctc gcctcgcccg cgatctcacc tatgcgcgcc
      721 tcgacctggc gctgctgcgg cgtctgcgga aacggcaggt actcgccgtt cgacaccacc
      781 tgggtgggga tcggcgagcg cagctcgtcg gcctcatcgg cgcgcgccag tccggaaagc
      841 tgggagtcgg acagccagga gtcttcttcg cgtgaaccgg gcaggcgttt cggcatcgga
      901 ccctcctcgc agggtgaacg ggccatacga tctgcctcat tccagcgcag tgcaagcccg
      961 catcagaagg gaaagcagat gagtccggtt aagggtatat ataggcccat gaataaattc
     1021 gcggtctgcc ttctcgccgg cgtcctgccg ttcgcgcttg ccgcctgcgc gagcgcaccg
     1081 ccgtctcagc ccggcagggc gtactcggcg gtaagcgaaa cggtggcggg cgacgccgca
     1141 gaccaggcgc tcaagatgct gggaagaccc tatcgcgccg gcggctccac gcctgccgcc
     1201 gggttcgatt gcagcgggct ggtgcagttc agctttcgac aggcgggcct ggcgctgccc
     1261 cacaacacgg ataagcaacg ctcggtttcg cgtcccgtgc gggtggcaga tctgcggcgc
     1321 ggcgacctgc tgttcttcga ccaggaaggc aagaagagct cgcacgtcgg catctatgtc
     1381 ggcgccggga aattcgtcca cgccccgtcc tcgggcaagc acgtgcgcag cgaccggctc
     1441 gactcgccct actggaagaa gcatctctcc ggagcacggc gcctttctgt atttgatgct
     1501 tgagctcccc gattttctag gtgaaataaa aggcattgac gggctgatcg gcgcccggaa
     1561 ggagacccgc atggcaaagg cctactggat cacctgctac cgctcgatca agaaccccga
     1621 cgcgctcgct gcctacgcga agctcgccgg ccccgcgatc caggccgcgg gcgggcgctt
     1681 cgtcgtgcgc ggcatgcccg ccaagaccta cgaagccggc ctggaccagc gcaccgtggt
     1741 cacggaatgg gagagcgtcg ccaaggcgat cgcggcgcac gacagcccgg gctaccagga
     1801 ggcgctgcgc gcgctgggcg gcgccgccga gcgcgacgtg cgcatcgtgg aaggcgcctg
     1861 agccatggcc gcagtccagc agatggtgga tccgcacgat gtcgtgatga cgggcgaaaa
     1921 ctcgttcatc cgcctctccg acgacggcgg caagagcctc gtcgagcgcg tcagccactg
     1981 gcgcgtgctg tggtcgcccg cgggacaagg acacgcgctc ttcatcgaga gttctttgct
     2041 gaaaacaatt tcggtctatg ccgacaacgc cggcctggtg cgctacctcc agggcacgat
     2101 cgagacgctg ctgcataagc cgttcgccga cgagtcgctg cccgtggttg acgccaggtt
     2161 cgagcgcagc ggcaactccc tcagcaccgt cgaagagcgc gtcgtctccc gcaaggacga
     2221 gatcatcctc gcctggtggg acctgatcgc gcccttcgtc ctcaccatgc cgccgggcgc
     2281 gctgaaacgg cccctcggcg tctacagcac cttcatcccc gcgaagagcg cgcagctttc
     2341 ggtgaacggc caagccgcca ccgccaaagt atttccgcag gagcgcttcg gcaggccttc
     2401 gtcgagctgc tgcctggcgt ggtcggagag ctggacccgg ccgagaggct gagacatgga
     2461 gcgcgcatga agctcaagct ggtatcgctg gccgtcgccg tggtcgcggt ggcctggctg
     2521 ggcatgtggg tcagcgatac cggcctgctc gtctactcgg ccgaggcccg ggtgcccctc
     2581 gcccgcgatt gccgctacct gatcggcgtc acggtgctga agcgcatcga gccgctcgct
     2641 cagcgctgcg aggtcctgag aacggtagga aggtaaccgt aaaaaatggg gacagacccc
     2701 attttccgtt gctcagcgca gcccgcggga aaatagggtc tgtccccatt ttttatggtt
     2761 cgcatgaaag tcgccgcgtt tctcttcgcc gttctgtttt ccggttgtgc attcggtcag
     2821 accatgcgaa tcgtcgtccc gttcccgccc ggcggcggcg ccgacgcgct cgcgcgcctg
     2881 atggcgccga agctcgccga aatttgga
//
LOCUS       DASPXW010000379         2568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_114545, whole genome shotgun sequence.
ACCESSION   DASPXW010000379 DASPXW010000000
VERSION     DASPXW010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114545"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..1031)
                     /locus_tag="VEL04_08795"
     CDS             complement(78..1031)
                     /locus_tag="VEL04_08795"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYT48505.1"
                     /translation="MGCVRAVLGFLGALLATAALAQYPVKPIRMVAPFPAGGALDVVG
                     RIIAVPMSQSLGQPVVVENRPGADGAIAADHVAKSAADGYTLFLVSYTVLSALPNIRK
                     NIPFDPIGDFTPISSVGKLSFFLAVHPSLPASKAEELIRYARANPGKLNYGSANATSM
                     LGPALLASFAGLDMHAVPYKGEALALNDLLSGRLQLMFISGTLLPHVKDGKLRALATT
                     LDSRSPLLPKVPTMVEAGLPPFPINPWIALVAPAKMPKDIVERLSRETNAALRRPEVR
                     TQLDNQAFEAVGSTPGEMGAVMRQQLEAWRRTISELRIKID"
     gene            1094..>2568
                     /locus_tag="VEL04_08800"
     CDS             1094..>2568
                     /locus_tag="VEL04_08800"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HYT48506.1"
                     /translation="MFDFVAKHRRFLQIFLGLIAITFATWGIESYTRFRGGRDTVASV
                     NGLEISQREFAEQMRVQQEQVRRMFGGSIDAAALDTPEARQALLDSLIAQRVVGSEAA
                     KQHLFMTRSAVIEAITQAPEFQENGRFSPARYSAYLASRGTTDQRNVAELQSQLPLAR
                     FVGSIADTGIAPRAVTARLAALEAQKREVSEVRIPAQQFLAQVKIDEAQVKAHYDAHQ
                     ADYRTPERVRAEYVVLSAQNLARGEPVSEAEIKAAYDARVSQYRIEESRRAGHILVKS
                     KEEADKLISEVRKNPARFAELARKHSLDAGSAEKGGDLGVVSKDSLASPALAEALFSM
                     KQGEVQVAQSEFGFHVVRVTGIQAAKSKSYDEVRKELADEIARQKGARKFAEAAENFS
                     NLVYEQPESLKPAAERFKLQIQATGWIAKSASQELGALDNPKLLAALFSADAIKDKRN
                     TDAIEVAPNTLVAARVTEHQPAAQRKFEEVKGEIAEGLRRRA"
BASE COUNT          465 a          830 c          891 g          382 t
ORIGIN      
        1 ggccgtcgtg gaaggccgga cctcgaacgg gattcctttc cgcggatcgg acgcggtcca
       61 ggtgctgcac taaacgtcta gtcgattttg atccgcagct cgctgatcgt gcgacgccac
      121 gcttcgagct gctggcgcat gaccgcgccc atctccccgg gagtcgatcc gaccgcctcg
      181 aacgcctggt tgtcgagctg ggtgcgcact tccggccggc gcagcgccgc gttcgtctcg
      241 cgcgagagcc gctcgacgat gtccttcggc atcttcgctg gcgcgacgag ggcgatccac
      301 gggttgatgg gaaacggagg aaggcccgct tcgaccatgg tcgggacttt gggcagcaac
      361 gggcttcttg aatccagggt ggtggcaagc gcgcgcagct tgccgtcctt cacgtgcggt
      421 agcagcgtgc ccgagatgaa catgagctgc aagcggccgc tgagcagatc gttcagcgcc
      481 agagcctcgc ccttatacgg tacggcgtgc atgtcgaggc cggcgaagga ggctagcagc
      541 gcgggcccga gcatgctggt cgcgttggcg ctgccgtagt tgagcttccc cgggttggcg
      601 cgcgcgtaac ggatcaactc ctccgccttg ctggcgggca gactgggatg aacggcgagg
      661 aagaatgaaa gcttgccgac cgaggagatc ggggtgaagt cgccgatcgg gtcaaaggga
      721 atgttcttgc gaatgttggg aagcgccgag agcacggtgt aggaaacgag gaacagggta
      781 tagccgtcgg ccgcggactt ggccacatgg tcggcggcga tcgcgccgtc ggcgccgggg
      841 cggttctcga cgacgaccgg ctgcccgagg gactgcgaca tcggcaccgc gatgatcctg
      901 ccgaccacgt cgagcgcgcc gcctgccggg aagggcgcca ccatccggat cggcttgacc
      961 gggtactgcg caagcgcagc cgtcgcaagc agcgcaccga gaaacccaag cacggctcga
     1021 acgcaaccca tgctcccctc cgctcaggac tgcgcaaaag tctacgccat ccccagtaca
     1081 attcccggct tccatgttcg atttcgtcgc caagcaccga cgcttcctcc agatcttcct
     1141 cggcctgatc gcgatcacct tcgccacctg gggaatcgag tcctataccc gcttccgcgg
     1201 cgggcgcgac acggtggcct cggtcaatgg cctcgagatc tcccagcgcg agttcgccga
     1261 gcagatgcgc gtgcagcagg agcaggtgcg ccgcatgttc ggcggctcga tcgacgccgc
     1321 ggcgctcgac acgccggagg cgcggcaggc gctgctcgac tcgctgatcg cgcagcgcgt
     1381 ggtaggcagc gaggcggcga agcagcatct gttcatgacc cgcagcgcgg tgatcgaggc
     1441 gatcacgcag gcacccgagt ttcaggagaa cggcaggttc tcgcccgcgc gctactccgc
     1501 gtacctggcg agccgcggca ccacggacca gcgcaacgtc gccgagctgc agtcgcagct
     1561 gccgctcgcg cgcttcgtcg gctcgatcgc ggacaccggc atcgcgccgc gcgcggtgac
     1621 ggcccggctg gcggcgctgg aggcgcagaa gcgcgaggtg tcggaggtgc gcattccggc
     1681 gcagcagttc ctcgctcagg tgaagatcga cgaggcgcag gtcaaggcgc actacgacgc
     1741 ccaccaggcc gactaccgca ctccggagcg cgtgcgcgcc gagtacgtcg tgctttcggc
     1801 ccagaacctg gcgcgcggcg agcccgtgag cgaggccgaa atcaaggccg cctatgacgc
     1861 gcgcgtctcg cagtaccgca tcgaggaaag ccgccgcgcc ggccacatcc tcgtgaagtc
     1921 gaaggaggaa gcggacaagc tgatttccga ggtgaggaaa aatcccgctc gcttcgcgga
     1981 gctggcgagg aagcattcgc tcgacgccgg ctcggccgaa aaaggcggcg acctcggcgt
     2041 cgtgtcgaag gactcgctcg ccagcccggc gctggcggaa gcgctgttct cgatgaagca
     2101 gggcgaggtg caggtcgcgc agagcgagtt cggcttccac gtggtgcgcg tcaccggcat
     2161 ccaggcggcg aagtcgaaat cctacgacga ggtaaggaag gagctcgccg acgagattgc
     2221 gcggcagaag ggcgcgagga aattcgccga ggcggccgag aatttcagca acctggtgta
     2281 cgagcagccc gaaagcctga agccggccgc cgagcgcttc aagctgcaga tccaggcgac
     2341 cggctggatc gcgaaatcgg ccagccagga gctcggcgcg ctggacaatc cgaagctgct
     2401 ggcggcgctg ttttctgcgg atgcaatcaa ggataaacgc aataccgacg ccatcgaagt
     2461 ggcgccgaac accctggtcg ccgcccgcgt caccgagcac cagccggccg cgcagcgcaa
     2521 gttcgaggag gtcaagggcg agatcgccga aggcctgcgc cggcgtgc
//
LOCUS       DASPXW010000380         2533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_992835, whole genome shotgun sequence.
ACCESSION   DASPXW010000380 DASPXW010000000
VERSION     DASPXW010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992835"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VEL04_08805"
     CDS             complement(<1..367)
                     /locus_tag="VEL04_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYT48507.1"
                     /translation="MKFDDVLVVGGAGFIGRHLVAALAARGIRVTVPSRRRESAKHLI
                     LLPTVDVIEADVMARGALERLAAGRQAVINLVGTLHGPNFSRVHVELPQAIVNACRAV
                     GVKRLLHVSALGASPSAPSE"
     gene            complement(371..2251)
                     /locus_tag="VEL04_08810"
     CDS             complement(371..2251)
                     /locus_tag="VEL04_08810"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HYT48508.1"
                     /translation="MAANGADEAVLGAYDAYRAGDPIKFAKHARALDGHVLAPWLDYW
                     RLSMRLEDAPIAEVREFLSRNSDAYVAELLRGEWLRVLGKRGEWQEFGREAAAYGEDE
                     LEVRCYAWIKRLEHGDDSAFAETGAMWLEPKEFPEGCAKLADTMVVRGRVSTTDIWQR
                     ARVLFENGQITAAKTALGYLRKDDAPDERSLTEAARRPRRLIERLPASLELRPAREVA
                     VLAAVRYARDEPQAAAEALEGALGRRLPEKELKYLWARVAFEAAREHHPDALKWYARA
                     GDTPLDDDQLAWKVRAALRAGAWHAVRDSIDRMSVEERQIATWTYWYGRALAFEGEEA
                     GSRAYYLRIAGQLEFYGLLANEELGHVFALPRDSYVPSEQEVEAAGREPGLARALELI
                     RLGLRTEGVREWQFATRYDDDPKLLAAAELARRAEVYDRAIHSADRTVRLHNFALRYP
                     VPFREVFGEYAKTHGLDEAWVLGLVRQESRFITEARSTAGAAGLMQIMPRTARYVASR
                     IGLRNYAKSVTEVQTNVTLGTGYLKLVLDQLGHPVLASAAYNAGPGRARRWRDAEKRL
                     EGAIYAETIPFGETRRYVKKVMANSVFYGELLENGLKPLKLRLGTIAPRGAAAAVDDD
                     LP"
     gene            2331..>2533
                     /locus_tag="VEL04_08815"
     CDS             2331..>2533
                     /locus_tag="VEL04_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48509.1"
                     /translation="MDPATLKSWRKSQRERLIAARAALAPAVLEAWRQRIDGFLELSF
                     PGLASCRLAFCWPVKDEYDARHFA"
BASE COUNT          386 a          889 c          843 g          415 t
ORIGIN      
        1 attcggatgg cgccgagggc gaggcgccca gcgcgctgac gtggagcaag cgcttcactc
       61 caaccgcgcg gcaggcgttg acgatggcct gcggcagctc gacatgcacg cgggagaaat
      121 tcggtccgtg cagcgtgccg accaggttga tcaccgcctg ccgtccggcg gcaagccgct
      181 cgagcgcgcc cctcgccatg acgtcggctt cgatcacatc gaccgtcggc agcaggatca
      241 ggtgcttggc gctctcgcgg cgccgcgacg gcaccgtgac ccggatgccg cgcgcggcga
      301 gcgccgcgac caggtgccgg ccgatgaagc cggccccgcc gactaccagg acatcgtcga
      361 atttcactcg ctacggcagg tcgtcgtcga cggctgcggc ggcaccgcgc ggcgcaatgg
      421 taccgaggcg cagcttgagc ggcttgagcc cgttttcgag cagctcgccg tagaacaccg
      481 aattggccat gacctttttc acgtacctgc gagtctcgcc gaaagggatg gtctcggcat
      541 agatcgcgcc ttccagccgt ttttcggcgt cgcgccagcg gcgcgcccgc cccgggcccg
      601 cgttgtacgc cgccgaagcg agcaccgggt ggccgagctg gtcgagcacc agcttcagat
      661 aacccgtgcc gagggtgaca ttggtctgta cctcggtgac gctcttcgca tagttgcgca
      721 gcccgatcct ggatgcgacg tagcgggcgg tgcgcggcat gatctgcatc aggccggcgg
      781 cacccgcggt cgagcgcgcc tccgtgataa agcggctttc ctggcgcacc aggccgagca
      841 cccaggcctc gtcgagaccg tgcgtcttgg cgtactcgcc gaacacctcg cggaagggca
      901 ccgggtagcg cagcgcgaaa ttatgcagcc gcacggtgcg atcggcgctg tggatggcgc
      961 ggtcgtacac ctcggcgcgg cgcgcgagct cggccgcggc gagcagcttc ggatcatcgt
     1021 cgtaacgggt cgcgaactgc cattcgcgca cgccctcggt gcgcagcccg agacggatca
     1081 gctcgagcgc gcgagcgagc ccgggctcgc gccccgccgc ctcgacttcc tgttcgctcg
     1141 gcacgtagct atcgcgcggc agcgcgaaga cgtggccgag ctcctcgttg gcgagcaggc
     1201 cgtagaactc cagctggccg gcgatgcgca ggtagtacgc gcggctgcct gcttcctcgc
     1261 cttcaaaagc gagcgcgcgc ccgtaccagt aggtccaggt cgcgatctgc cgctcctcca
     1321 cggacatgcg gtcgatcgaa tcgcgcacgg cgtgccatgc gccggcgcgc agcgccgcac
     1381 gcactttcca ggcgagctga tcgtcgtcga gcggcgtgtc gccggcgcgc gcataccact
     1441 tcagcgcatc ggggtggtgc tcgcgcgccg cctcgaaagc gacgcgggcc cagagatatt
     1501 tcagttcttt ctcgggcagc cgccgcccga gcgctccctc cagcgcttcg gccgcagcct
     1561 gcggctcgtc gcgcgcatag cgcactgcgg cgagcaccgc gacttcgcgc gccgggcgca
     1621 attcgaggct cgcgggcagg cgctcgatca ggcgccgcgg gcggcgcgcc gcctcggtga
     1681 gcgagcgctc gtcgggcgca tcgtctttgc gcaggtagcc gagcgccgtc ttcgcggcag
     1741 tgatctgccc gttctcgaac agcactctcg cccgctgcca gatatcggtg gtcgacaccc
     1801 ggccgcgcac caccatggtg tcggcgagct tggcgcagcc ctcgggaaat tccttcggct
     1861 cgagccacat cgcgccggtc tcggcgaatg cgctgtcgtc gccatgctcg aggcgcttga
     1921 tccaggcgta gcagcgcacc tcgagctcgt cctcgccgta ggccgcggcc tcgcgcccga
     1981 attcctgcca ttcgccgcgc ttgccgagta ccctgagcca ctcgccgcgc agcagctcgg
     2041 cgacataggc gtcggaattc cttgacagaa attcacgtac ctcggcgatc ggcgcgtcct
     2101 cgagccgcat cgacagccgc cagtagtcga gccacggcgc gagcacgtgg ccgtcgagcg
     2161 ctcttgcgtg tttggcgaac ttgatcggat cgccggcgcg gtaggcgtcg taggcgccaa
     2221 gcactgcctc gtccgcgccg tttgccgcca acgccggcgc agaaacgact gctaggagta
     2281 gcgcaagatt gcggaaaacc ttgaaaatca taaaactaag cgaatccccc atggatccgg
     2341 ccaccttaaa atcctggcgc aagtcgcagc gggagcggct gattgccgcg cgagccgcgc
     2401 tggcgccggc cgttcttgaa gcctggcgtc agcgcatcga cggtttcctg gagctctcgt
     2461 tccccggact tgccagttgt cgcctcgcgt tttgttggcc ggtcaaagac gagtacgacg
     2521 cccgccattt tgc
//
LOCUS       DASPXW010000381         2329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1251065, whole genome shotgun sequence.
ACCESSION   DASPXW010000381 DASPXW010000000
VERSION     DASPXW010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251065"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VEL04_08820"
     CDS             <1..314
                     /locus_tag="VEL04_08820"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HYT48510.1"
                     /translation="AEMLAALSGRSHEVLSAVSLKYADWVEGGLSVSEVRFRPLGEEE
                     IRQYVASGECDDKAGAYAIQGRAARFIAELRGSYSGVMGLPLYETSELLEKLRGRRDP
                     H"
     gene            292..1749
                     /gene="rng"
                     /locus_tag="VEL04_08825"
     CDS             292..1749
                     /gene="rng"
                     /locus_tag="VEL04_08825"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011545847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HYT48511.1"
                     /translation="MAEEILINVTPQETRVAVAGSGVVQELLIERAATRGLVGNIYSG
                     RVARVLPGMQSAFVEIGLERAAFLHVADIRENKDSSRPIEKVLAEGQPILVQVVKDPI
                     GTKGARLSTQISVAGRLLVYLPYDPHIGISQRIEDEEGRQLLRERLKELVPADEKGGF
                     IVRTLAETAAEEELRADLEYLCHLWRVIRERSSDGNAGAEPPQLIYQDLSLAQRVLRD
                     MVSADTSRVVVDSRDTWQKLAAFAEHHMPRVRGKLEHYTGERPLFDLYNVETEIERAL
                     SRRVELKSGGTLIIDQTEALTTIDVNTGGFVGSRNFDDTIFKTNLEAAQAIARQLRLR
                     NLGGIIIVDFIDMLSSEHRAAVLEEFGRALARDRTRITVNGFTALGLVEMTRKRTRES
                     LAHVLCEPCPTCGGRGEVKTPHTVCYEILREILREARAFKAREYRVLAAQPVIDLLLE
                     DESSSLAMLSDFISKPISVQVETSYTQEQFDIVLM"
     gene            1746..>2329
                     /locus_tag="VEL04_08830"
     CDS             1746..>2329
                     /locus_tag="VEL04_08830"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HYT48512.1"
                     /translation="MTELCFTPARKLAQMLRARRVSATEVVEAFIAQIERVNPKVNAV
                     VTFVPELALAEARKIDRKKKNNTPFAGLPIAYKDLTPTKGIRTTFGSLVFEKNIPAEN
                     HAIVDRLAAAGVVTLGKTNTPEFGAGSQTFNKVFGATRNPYDLAKTCGGSSGGAATAV
                     ACGMLPFADGGDLAASLRNPGNYCNVVGFRPTPCR"
BASE COUNT          415 a          796 c          758 g          360 t
ORIGIN      
        1 ccgccgagat gctcgccgcg ctgtcgggcc gcagccacga ggtgctgagc gccgtatcgc
       61 tcaaatacgc cgactgggtc gagggcgggc tctcggtgtc cgaggtgcga ttcaggccgc
      121 tcggcgaaga ggagatcagg cagtacgtgg cgagcggcga atgcgacgac aaggccggcg
      181 cctacgcgat ccagggccgc gcggcgcgct tcatcgccga gctgcgcggc agctattccg
      241 gcgtcatggg cctgccgctg tacgaaacca gtgaattgct tgaaaagctg cgtggccgaa
      301 gagatcctca ttaacgtcac gccgcaggag acgcgcgtcg ccgtcgccgg ctctggcgtg
      361 gtgcaggagc tgctgatcga gcgcgcggcg acccgcggcc tggtcggcaa catctacagc
      421 gggcgcgtgg cgcgggtgct gcccggcatg cagtcggcgt tcgtcgagat cgggctcgag
      481 cgcgccgcct tcctgcacgt cgccgacatc cgggaaaaca aggattcctc caggcccata
      541 gagaaagtcc tcgccgaagg gcagccgatc ctggtgcagg tggtgaagga cccgatcggc
      601 accaaaggcg cgcgcctttc gacccagatc tcggtcgccg ggcggctgct ggtgtacctg
      661 ccgtacgacc cgcacatcgg catctcgcag cgcatcgagg acgaggaggg caggcagctc
      721 ctgcgcgagc ggctgaagga gctggtgccc gccgacgaaa aaggcggctt catcgtgcgc
      781 actctcgccg agaccgcggc cgaggaagag ctgcgcgccg acctcgaata cctgtgtcac
      841 ctgtggcgcg tgatccgcga gcgctcttca gacggcaatg cgggcgccga gccgccgcag
      901 ctgatctacc aggatttgtc gctggcgcag cgcgtgctgc gcgacatggt gagcgcggac
      961 acttcacgcg tggtggtcga ctcgcgcgac acctggcaga agctcgccgc cttcgccgag
     1021 caccacatgc cacgggtgcg cggcaagctc gagcactaca ccggcgagcg gccgttgttc
     1081 gacctctaca acgtcgagac cgagatcgag cgggcgctct cgcgccgggt cgagctcaag
     1141 tcgggcggca ccctgatcat cgaccagacc gaggcgctga ccaccatcga cgtcaacacc
     1201 ggcggcttcg tcggcagccg caacttcgac gacaccatct tcaagaccaa cctcgaggcg
     1261 gcgcaggcga tcgcacgcca gctcaggctg cgcaacctcg gcggcatcat catcgtcgac
     1321 ttcatcgaca tgctgtcgag cgagcatcgc gcggccgtcc tcgaagaatt cgggcgcgcc
     1381 ctcgcccgcg accgcacgcg catcacggtg aacggcttca ccgcgctcgg gctcgtcgag
     1441 atgacgcgaa aacgcacccg agagtcgctc gcgcacgtgc tgtgcgagcc ctgcccgacc
     1501 tgcggcgggc gcggcgaggt caagaccccg cacaccgtct gctacgagat cctgcgggag
     1561 atcctgcgcg aggcgcgcgc cttcaaggcg cgcgagtacc gcgtgctcgc cgcccagccg
     1621 gtgatcgacc tgctgctcga ggatgagtcg agctctctcg ccatgctctc ggactttatc
     1681 agcaagccga tctcggtgca ggtcgagacg agctacaccc aggaacaatt cgacattgtc
     1741 ttgatgtgac ggaactctgc tttacccccg cccgcaagct cgcgcagatg ctgcgcgcgc
     1801 gcagggtctc ggccaccgag gtggtcgagg ctttcatcgc gcagatcgag cgcgtcaacc
     1861 cgaaggtcaa cgcggtggtc acgtttgttc ccgaacttgc tcttgcggaa gcgcgcaaga
     1921 tcgacaggaa aaagaagaac aacaccccgt ttgccggact gccgatcgcc tacaaggatc
     1981 tgacgccgac gaaaggtatc cgcaccacct tcggctcgct ggttttcgag aagaatattc
     2041 ctgccgaaaa tcacgcgatc gtcgatcggc ttgccgctgc cggcgtcgtc acgctcggca
     2101 agaccaacac tccggaattc ggcgccggca gtcagacctt caacaaggtg ttcggcgcga
     2161 cgcgcaaccc gtacgacctc gcgaagacct gcggcggctc ctcgggcggc gcggccaccg
     2221 ccgtcgcttg cggcatgctg cccttcgccg acggcggcga cctcgccgcg agcctgcgca
     2281 acccgggcaa ctattgcaac gtggtcggct ttcgcccgac gccctgccg
//
LOCUS       DASPXW010000382         2213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_488619, whole genome shotgun sequence.
ACCESSION   DASPXW010000382 DASPXW010000000
VERSION     DASPXW010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488619"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="VEL04_08835"
     CDS             complement(<1..354)
                     /locus_tag="VEL04_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HYT48513.1"
                     /translation="MKPELLPSGGVHQALAHDSAALHVAGEARYVDDLPEPPGTLHAA
                     LGVSSETLADLRSIDLDAVRRAPGVVAVLAAADIPGKNNIGPVIADEPVFAAERVEFR
                     GQPIFAVAATSVELAR"
     gene            complement(351..1757)
                     /gene="xdhA"
                     /locus_tag="VEL04_08840"
     CDS             complement(351..1757)
                     /gene="xdhA"
                     /locus_tag="VEL04_08840"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase small subunit"
                     /protein_id="HYT48514.1"
                     /translation="MRSCLDFILNGERVSLRDEDPTRTLLTWLRESRRLLGTKEGCAE
                     GDCGACTVVLAERGASGALDYLPVNSCLLFLGALDGREVISVDALKCGAALHPVQQAM
                     VDCHGSQCGFCTPGFVMALYAHHKKNPPGKRQDLCDALAGNLCRCTGYRPILDAGSRM
                     AALADAKRERAEDAARLTSLASLPRDATLVETGGKRFFAPIWADSLAEFLVEHPQATI
                     LAGGTDLGLLVTKAHRDLHDLVYVGRVAALKEIAEREAEIEIGAAAPLAVAQSALGGI
                     HPDIGELLRRFGGAQVRACGTLGGNLANASPIGDSMPVLLALDASISLQQGKNIRQVK
                     LAAFYAGYRKTVLRPGEFIRSVRVPKLASGARFAAYKLAKRFDQDISGVCAAFYADSH
                     EARFGFGGMAATPARARRAEAVYAEGVDAACRALEQDFKPLSDHRASSWYRLTAAQNL
                     LRKFHAGAPPRALLELVA"
     gene            1854..>2213
                     /locus_tag="VEL04_08845"
     CDS             1854..>2213
                     /locus_tag="VEL04_08845"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HYT48515.1"
                     /translation="MKQADMMYLRRAIALAQSSRDEGQRPFGALVVLDAEVLADSGRR
                     PSGGRDATAHCEIRIVRAVAQQHPPAALARATLYASAEPCAMCAGAVYWSGIGRVVFG
                     LSEAKLRALTGNHPENPT"
BASE COUNT          354 a          738 c          789 g          332 t
ORIGIN      
        1 ccgcgccagc tcgacgctcg tcgcggcgac ggcaaagatc ggctggccgc ggaactccac
       61 gcgttcggcg gcgaacaccg gctcgtcggc gatcaccggc ccgatgttgt tcttgcccgg
      121 gatgtccgcg gcggcgagca ccgccaccac gcccggcgcc cggcgcaccg catcgaggtc
      181 gatcgagcgg aggtcggcca gcgtctcgct cgagacgccg agcgcggcgt gcaacgtgcc
      241 cggcggctcg ggcaggtcat cgacgtagcg cgcctcaccc gcaacatgca gcgccgcgct
      301 gtcgtgcgcg agcgcctggt gcacgcctcc ggagggaagc agttcgggct tcacgcgacg
      361 agctcgagga gcgcgcgcgg cggcgccccg gcgtggaact tgcgcagcag gttctgcgcc
      421 gcggtgagcc ggtaccagct cgaggcgcgg tgatccgaga gcggcttgaa atcctgctca
      481 agggcgcggc acgctgcgtc gacgccctcg gcgtatacgg cctctgcgcg ccgggcgcgc
      541 gccggcgtgg cggccatgcc gccgaacccg aacctggctt catgcgaatc agcataaaag
      601 gcggcgcaga cgccggaaat gtcctggtcg aagcgcttcg cgagcttata ggcggcgaag
      661 cgcgcgccgg acgcgagctt cggcacgcgc accgagcgga tgaattcgcc gggccgcagc
      721 accgtcttgc ggtagccggc atagaaggca gcgagcttca cctgacgaat gttctttccc
      781 tgttgaagag aaatcgatgc gtccaatgcc agcaacaccg gcatggaatc gccgatcggc
      841 gaggcattgg cgaggttgcc gccgagcgtg ccgcaggcgc gcacctgcgc gccgccgaag
      901 cggcgcagca gctcgccaat gtcgggatgg atgccgccca gagcggactg cgccacggcg
      961 aggggcgcgg cggcgccgat ctcgatttca gcttctcttt ctgcaatttc cttcaaggcg
     1021 gcgacacggc cgacgtagac caggtcgtgc aggtcgcggt gcgccttggt gacgagcagg
     1081 ccgagatcgg tgccgcccgc gaggatcgtg gcctgcgggt gctcgaccag gaattctgca
     1141 agtgaatcag cccaaatcgg cgcgaagaag cgcttgccgc cggtttcgac cagcgtggcg
     1201 tcacgcggca gcgaggccag gctggtgagg cgcgcggcgt cttcggcacg ctcgcgcttc
     1261 gcgtcggcga gcgcggccat gcggctgccg gcgtcgagga tcggccggta gccggtgcag
     1321 cggcagaggt tgccggctag cgcatcacaa agatcctgcc ttttgccggg gggatttttt
     1381 ttgtgatgcg catacagggc catcacgaag cccggcgtgc agaagccgca ttgcgagccg
     1441 tggcagtcga ccatcgcctg ctgcacggga tgcagggcag cgccgcattt cagcgcatcg
     1501 acgctgatca cctcgcggcc atcgagcgcg ccgaggaaca gcaggcagct gttgaccggc
     1561 aggtagtcga gcgccccgct ggcgccgcgc tccgcgagca ccacggtgca ggcgccgcaa
     1621 tcaccctcgg cgcagccttc cttggtgccg aggagccgcc gcgactcgcg cagccacgtg
     1681 agcagcgtgc gggtcgggtc ttcgtcgcgc agcgaaaccc gctcgccgtt gagaatgaag
     1741 tcgaggcagc ttctcatcgg gacgcccagc gtaggctcgc ctgccgcaga ttgtcaacca
     1801 aatagcctca gattgttgac aatctgtctg cgcgcgcata gcctcgggcg agcgtgaagc
     1861 aagcggacat gatgtatctg cggcgtgcga ttgcattggc gcaaagctcg cgcgacgaag
     1921 gccagcggcc tttcggcgcg ctggtggtgc tcgacgccga agtgcttgcc gacagcgggc
     1981 gcaggccttc cggcgggcgc gatgccacgg cgcattgcga gatccgcatc gtgcgggccg
     2041 tggcgcagca gcaccctccg gcagccctcg cacgcgccac gctttacgcc agcgccgagc
     2101 cctgcgcgat gtgcgccggg gcggtctact ggtcggggat cggtcgcgtg gtgttcggtc
     2161 tcagcgaggc gaagctccgc gcgctgaccg gcaatcatcc ggagaatccg acg
//
LOCUS       DASPXW010000383         2507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_743160, whole genome shotgun sequence.
ACCESSION   DASPXW010000383 DASPXW010000000
VERSION     DASPXW010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743160"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VEL04_08850"
     CDS             <1..348
                     /locus_tag="VEL04_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006163416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYT48516.1"
                     /translation="HYDYKSEHPLNKEFVKVFNEINGRNPDFFSIGGYDGMHAIYEAL
                     KKTGGNAAGDALIAAARGSKWESPRGPMSIDPETRDVVQTIYIRRVQKVGNEVVNVPF
                     DKIENVKDPTPAR"
     gene            389..1252
                     /locus_tag="VEL04_08855"
     CDS             389..1252
                     /locus_tag="VEL04_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYT48517.1"
                     /translation="MFGSLLGVLFDGLAYGMLLFLLSVGLSVTLGMMNFVNLAHCSFA
                     MIGGYVTVTLMNELSWPFFATLPCAFAAAAVASVALERTLYRRLYRAGELDQCLLTIG
                     IVFVSVAVAAYIYGTIQQPVQLPSYLRGSVTLMGMTFGVYRLFLIAAGLALTLVLVAA
                     LEWTRFGAQVRAAVDNQRMARGLGINVDGVFAVTFALGSGLAGLGGALAIDVVGLDPA
                     FAFTYLVYVLIVVSVGGLGSIGGSFAAATLLGISDMAGKYYFPELGAFLIYLVMVALL
                     MWRPAGLFGRR"
     gene            1252..2244
                     /locus_tag="VEL04_08860"
     CDS             1252..2244
                     /locus_tag="VEL04_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010398393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYT48518.1"
                     /translation="MTAAAEQIAAPHRHLEAQIRWRAIETVFWLATLLPLVASPSYLV
                     LASQIAITALFALSLDLILGYAGIVSLGHAAFFGLGAYTAGLAAKHGWGEPLTGLVLA
                     AGVAGLVGYASSFIIARFRHLALIMITLGMGLLLLEAANSASWLTGGADGLQGVRMWP
                     LLGTFRFDLWGYTAYAYSLIVLFLTFLLVRRLIHSPFGLALRGIRENATRMPAIGASS
                     RAHIRKVYTIAAVIAGIAGGLLTQTTETVSLGVLDFQRSADVLVILVLGGAGRLYGGL
                     IGAIIFMVARDQFSGINPQYWYFWIGTLLIAVVVFLPNGILGGLNRLMSRWGNR"
     gene            2241..>2507
                     /locus_tag="VEL04_08865"
     CDS             2241..>2507
                     /locus_tag="VEL04_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYT48519.1"
                     /translation="MSEPALATRALNKSFGSLAVARDVELTLPRGVRYALIGPNGAGK
                     TTLINLMTGMLEPDAGRIFLGPDDITRLEPEARVKRGLVRTFQIN"
BASE COUNT          337 a          876 c          823 g          471 t
ORIGIN      
        1 cactacgact acaagagcga gcatccgctg aacaaggagt tcgtgaaggt gttcaacgag
       61 atcaacggcc gcaacccgga cttcttctcg atcggcggct atgacggcat gcacgccatc
      121 tacgaggcgc tgaagaagac cggcggcaac gccgccggcg acgcgctgat cgcggccgct
      181 cggggatcga agtgggagag cccgcgcggc ccgatgtcga tcgatccgga aacgcgcgac
      241 gtcgtgcaga cgatctatat ccggcgggtg cagaaggtcg gcaacgaagt cgtcaacgtt
      301 ccgttcgaca agatcgagaa cgtcaaggac ccgacgccgg cccggtaacc gatagacgtc
      361 ggcggctcac cggaaggggc gggcggtcat gttcggctcc ttgctcggcg tgctgttcga
      421 cgggctggcc tacgggatgc tgctgttcct cctgtcggtc gggctgtcgg tgacgctcgg
      481 gatgatgaat ttcgtcaacc tggcgcactg cagcttcgcc atgatcggcg gctacgtcac
      541 cgtgacgctg atgaacgagc tgagctggcc gttcttcgcc acgctgccct gcgcgtttgc
      601 cgcggccgcg gtcgcgagcg tcgcgctcga gcgcacgctc taccggcggc tctatcgcgc
      661 cggcgagctc gaccagtgcc tgctgacgat cgggatcgtg ttcgtctcgg tcgcggtcgc
      721 ggcctacatc tacggaacga tccagcagcc ggtgcagctg ccgtcctatc tgcgcggctc
      781 cgtcacgctc atgggcatga cgttcggcgt ctaccggctg ttcctgatcg ccgccgggct
      841 cgccctcacc ctcgttctcg tcgccgcgct ggaatggacg cggttcggcg cgcaggtgcg
      901 cgccgcggtc gacaaccagc gcatggcgcg cggtctcggc atcaatgtcg acggcgtgtt
      961 cgcggtcacg ttcgcgctcg gcagcgggct cgcgggcctg ggcggcgcgc tcgcgatcga
     1021 cgtggtcggc ctcgacccgg cgttcgcctt cacctacctg gtctacgtcc tgatcgtggt
     1081 gtcggtcggg gggctcggct cgatcggcgg ctcgttcgcg gccgcgaccc ttctcggcat
     1141 cagcgacatg gccggcaagt attactttcc cgagctcggc gccttcctca tctatctcgt
     1201 catggtggcg ctgttgatgt ggcggccggc cggcctgttc gggaggcgct gatgacggcg
     1261 gccgccgagc agatcgccgc gccgcaccgc catctggaag cgcagatccg ttggcgggcg
     1321 atcgagaccg tgttctggct ggcgacgctt ctccccctcg tggcgagccc gtcctacctc
     1381 gtgctggcga gccagatcgc catcaccgcg ctgttcgcgc tctcgctcga tctcatcctg
     1441 ggctatgccg gcatcgtctc gctcggccac gccgcgttct tcgggctcgg cgcctacacc
     1501 gccggcctcg ccgccaagca cggctggggc gagccgctca ccgggctcgt gctcgccgcg
     1561 ggggtcgccg gtctcgtcgg ctatgcctca agcttcatca tcgcgcgctt ccgtcacctc
     1621 gcgctgatca tgatcacgct cggcatgggt ctcctgctgc tcgaggccgc caacagtgcg
     1681 agctggctca ccggcggcgc tgatggcctg cagggcgtgc gcatgtggcc gctcctcggc
     1741 acctttcggt tcgatctctg gggctatacc gcctacgcct attcgctgat cgttcttttc
     1801 ctgacctttc tgttggtccg gcgcctgatc cattccccgt tcggcctggc gctgcgcggc
     1861 attcgcgaga acgcaacccg catgccggcg atcggtgcct cgagccgcgc ccatatccgc
     1921 aaggtctaca ccatcgccgc cgtgatcgcc ggcatcgccg gcggcctgct cacccagacc
     1981 accgagacgg tctcgctcgg cgtcctggat ttccagcgct cggccgacgt gctggtgatc
     2041 ctggtgctgg gcggcgccgg ccggctctat ggcggcctga tcggcgccat catcttcatg
     2101 gtggcgcgcg accagttctc cggcatcaat ccgcaatact ggtacttctg gatcggcacg
     2161 ctcctgatcg ccgtcgtcgt gttcctgccg aacgggatcc tcggcgggct caaccggctg
     2221 atgtcgcgct ggggcaaccg atgagcgagc cggcgctggc gacgcgcgcg ctcaacaaga
     2281 gcttcggatc gctcgcggtg gcgcgggacg tcgagctcac gctgccgcgc ggggtgcgct
     2341 acgcgctgat cggccccaac ggcgccggca agaccaccct gatcaatctc atgaccggca
     2401 tgctcgagcc cgatgccggc cggatcttcc tcgggcccga cgacatcacc cggctcgagc
     2461 ccgaggcgcg cgtcaagcgc gggctcgtgc gcacgttcca gatcaac
//
LOCUS       DASPXW010000384         2361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_993490, whole genome shotgun sequence.
ACCESSION   DASPXW010000384 DASPXW010000000
VERSION     DASPXW010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993490"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VEL04_08870"
     CDS             <1..510
                     /locus_tag="VEL04_08870"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HYT48520.1"
                     /translation="ELSTEIRWLPFQLNPGMPREGMPRAAYRKAKFGSVERSRALDAR
                     VIAEGRSEGIDFAFDRIERTPNTVAAHQLIALAQNQNQVVDALFHAYFEEAKDIGDPA
                     MLAEIAEQCGVKGWPRQEGEKEVAVLEESLRGLGISAVPTFIFGRRLGVSGAQPAEAL
                     VAAMREAVG"
     gene            complement(507..1091)
                     /locus_tag="VEL04_08875"
     CDS             complement(507..1091)
                     /locus_tag="VEL04_08875"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYT48521.1"
                     /translation="MSPERFQAAIVYAELRNFTRLSEVLEPAKVLQLANDFFTLVAAT
                     VTANGGSVLSVQNDSIVGAFSGERQKFSKQALEAAQVVQREFGTLGERWQTDYGLPAA
                     VSLGVHLGEAVFGMAGPLGKQQFVAFGDCVSITERLVHRARTGEIVLSLDFMKALGAT
                     VQTLGAEELPPLELGKRPPIPIYGLVLETRLDFT"
     gene            complement(1104..1811)
                     /locus_tag="VEL04_08880"
     CDS             complement(1104..1811)
                     /locus_tag="VEL04_08880"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HYT48522.1"
                     /translation="MSVVSRMDRVVDAFVARCRWFHEPMSRGRARMFALQHRLNTRQR
                     DSVLKLKVAANCSDWETRMRIIRACSEEVIADHEHGGGRPHWQILEDLGVRIGLERRA
                     LRAAKPLPSTELAWAAWEGLMANRHWLEGIVANTCAERVNVPGYGTGVFRKRGWFGLE
                     RQRWKKLFDLSNHELEFFELHTAADIEHSNTGWRAVEKFAKQLGMEDAAVAACERNLY
                     VWQHYLDGIAAAGDTRR"
     gene            complement(1822..>2361)
                     /locus_tag="VEL04_08885"
     CDS             complement(1822..>2361)
                     /locus_tag="VEL04_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HYT48523.1"
                     /translation="GCESIEPLADNEYQVLMVARVGPVSAKFKGKLTLSDIRPPQSYA
                     IAFEGQGGPAGFAKGSAKVSLAAQGLSTKLSYDVKASVGGKLAQIGSRLVDAAAKKVA
                     DDFFRNFNEKVASLQPDPDATVIGHHKVMKQQKEEDDHQAGPVPRDPDLPDVSGTTLA
                     LFAAGLLVVFLAGLIALFG"
BASE COUNT          389 a          800 c          782 g          390 t
ORIGIN      
        1 gagctgagca cggagatccg ctggctgccg ttccagctca accccggcat gcctcgcgaa
       61 ggcatgccgc gcgccgcata ccgcaaggcg aagttcggct cggtcgagcg ctcgcgcgcg
      121 ctcgatgcgc gcgtgatcgc cgagggcagg agtgagggca tcgactttgc cttcgaccgc
      181 atcgagcgta cgcctaacac tgtcgccgca caccagctga tagcgcttgc acagaatcag
      241 aatcaagtcg tcgatgcgct attccacgcc tatttcgagg aagcgaaaga catcggcgat
      301 ccggccatgc tcgccgagat cgccgagcaa tgtggcgtga agggatggcc ccgccaggaa
      361 ggcgagaagg aagtcgcggt actggaagaa agcctgcgcg gccttggcat ctcggccgtg
      421 ccgaccttca tcttcgggcg caggctcggc gtctcgggcg cgcagcctgc cgaggccctg
      481 gtcgccgcga tgcgcgaagc ggtcggctag gtaaagtcca gccgggtctc gagcaccagg
      541 ccgtagatcg ggatcggcgg ccgtttcccc agctcgagcg gcggcagctc ctcggcgccg
      601 agggtttgca cggtggcgcc gagcgctttc atgaaatcga gcgacagcac gatctcgccg
      661 gtgcgcgccc ggtgcaccag gcgctcggtg atgctcacgc agtcgccgaa ggcgacgaac
      721 tgctgcttgc ccaagggtcc cgccatgccg aatactgcct cgcccagatg cacgccgagc
      781 gataccgccg cgggcaggcc gtagtcggtc tgccagcgct ccccgagcgt gccgaactcg
      841 cgctgcacca cctgcgcggc ttccagcgcc tgcttcgaga atttctgccg ctcgccgctg
      901 aaggcgccga cgatggaatc gttctgcacc gagagcacgc tgccgccgtt ggccgtcacg
      961 gtcgccgcca ccagcgtgaa gaagtcgttg gcgagctgca gcaccttggc cggctcgagc
     1021 acctcggaga gccgcgtgaa gttgcgcagc tccgcgtaca cgatcgcagc ctggaagcgt
     1081 tcgggcgaca tgcgaggtgg attctagcgc cgggtatcgc cggctgcggc gataccgtcg
     1141 aggtaatgct gccagacgta gagattgcgc tcgcaggccg ccaccgcggc gtcctccatg
     1201 ccgagctgct tcgcgaactt ctcgaccgcc cgccagccgg tgttggagtg ctcgatgtcg
     1261 gccgcggtgt gcaactcgaa gaactccaat tcatgatttg ataaatcaaa cagcttcttc
     1321 caccgctgcc gttcaagccc gaaccagccg cgcttcctga agacccccgt gccgtagccc
     1381 ggaacgttga cgcgctcggc gcaggtgttg gcgacgatgc cttcgagcca gtggcgattc
     1441 gccatcaggc cttcccaggc agcccaggcg agctcggtcg agggcagcgg cttggccgcg
     1501 cgcaaggcgc ggcgctcgag accgatcctc acgcccaggt cctcgaggat ctgccagtgc
     1561 ggccggccgc cgccgtgctc gtggtcggcg atcacctcct cgctgcaggc gcggatgatg
     1621 cgcatgcgcg tctcccagtc gctgcagttg gcggccacct tgagcttgag caccgaatcg
     1681 cgctgacgcg tgttcagccg gtgctgcagg gcgaacatgc gcgcccgccc gcggctcatc
     1741 ggctcgtgga accagcggca gcgcgcgacg aaggcgtcga ccaccctgtc catcctggaa
     1801 acgacgctca tgcgcgtgaa atcagccgaa gagagcgatc agcccggcca ggaacaccac
     1861 cagcaagccg gcggcgaaca aggcgagcgt cgtgcccgac acatccggca ggtcgggatc
     1921 gcgcggcacc gggccggcct gatggtcgtc ttcctccttc tgctgcttca tgaccttgtg
     1981 atgcccgatg accgtggcgt cggggtcggg ctgcagggaa gcgaccttct cgttgaagtt
     2041 gcggaagaaa tcgtcggcga ctttctttgc cgcggcgtcg accaggcgcg agccgatctg
     2101 cgccagcttg ccgccgacgc ttgccttcac gtcataggag agcttggtgc tgagtccctg
     2161 cgcggcgagg ctcaccttgg cgctgccctt ggcgaagccg gccgggccgc cctggccttc
     2221 gaacgcgatg gcgtaggact gcggcggcct gatgtcggaa agcgtgagct tgcccttgaa
     2281 cttcgcgctc accgggccga cgcgcgccac catcagcacc tggtactcgt tgtcggcgag
     2341 cggctcgatc gactcgcagc c
//
LOCUS       DASPXW010000385         2446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_743323, whole genome shotgun sequence.
ACCESSION   DASPXW010000385 DASPXW010000000
VERSION     DASPXW010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743323"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="VEL04_08890"
     CDS             complement(<1..766)
                     /locus_tag="VEL04_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018310601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYT48524.1"
                     /translation="MERIWLKSYPPGVPADIRYDEFKSVGDLVDHKVAEFRERPAFHS
                     MGKTISFGELDALSRAFGAWLQSKGYGKGVRVAIMMPNCLQYPVILYGTLRAGGVVVN
                     VNPLYTPRELEHQLKDSGAQVIVILENFATTLEQVLPKTPVKHVVLAALGDLLGLKGA
                     IVNFVVRKVKKMVPGFELPGALRFNAMLAEAAGRELRKVEVGPNDLAFLQYTGGTTGV
                     SKGAMLLHRNILANIEQAAAWLTPGLSGERAIIITAL"
     gene            complement(775..2073)
                     /locus_tag="VEL04_08895"
     CDS             complement(775..2073)
                     /locus_tag="VEL04_08895"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYT48525.1"
                     /translation="MRSSRSRTRRKACPLSSKNANRLSRTVSPAPVLLVAHILSMLGF
                     ATYAALLPGLRDEWALSNVQAGVVGGMFFAGYVATVSYWTALTDRLDGRKVYVGGSVI
                     AAAASAGFGLAADGFAAAVLFQALLGVGIAATYMPGLRLLSDRIAGPRQSRSIAFYTS
                     FFGIGTALSLALAGLVAPLAGWRMAFIVSAAGPLLAASLVFALIEPLPHAPRPTLGFS
                     LNTLFPVVAWRKVLSDRATAGYTLGYVAHCLELFGSRAWMVAFLAFSSALHGSETFPW
                     RLPAIAAVVNLLAVPASIVGNEIALRIGRRRWIMLAMAGSGASGVVVALAAPWHWGLV
                     LALLIAYSMLVMAESGTLTAGLVAAAPAELRGAAMGLYSLAGFGGGLLGPVIFGAALD
                     AGGGAAKGAAWVIAYAAIGAGCLAAPLVVKLTARKTSGPC"
     gene            complement(1991..>2446)
                     /locus_tag="VEL04_08900"
     CDS             complement(1991..>2446)
                     /locus_tag="VEL04_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HYT48526.1"
                     /translation="GGGNELAMQCDILIAAENAKFGQPEINLGVMPGAGGTQRLPRFT
                     SKAKAMDLCLTARMMDAAEAERAGLVSRVVPLDKLMAEALAAAQKIAACSLPVVIMIK
                     ESINRAYESSLAEGVRFERRLFHSQFALEDQKEGMSAFLEKRKPAFKNR"
BASE COUNT          428 a          838 c          832 g          348 t
ORIGIN      
        1 gcagcgcggt gatgatgatc gcgcgctcgc cgctgagccc cggcgtgagc caggccgccg
       61 cctgctcgat gttggcgagg atgttgcggt ggagcagcat cgcccccttg gagacgccgg
      121 tggtgccgcc cgtgtattgc agaaaggcga ggtcgttcgg accgacctcg acctttctca
      181 gctccctgcc ggccgcctcg gcgagcatgg cgttgaagcg cagcgcgccg ggcagctcga
      241 aacccggcac catcttcttc accttgcgca ccacgaaatt cacgatcgcg cccttcaggc
      301 cgagcaggtc gccgagcgcc gccagcacta cgtgcttcac cggggtcttc ggcagaacct
      361 gctcgagcgt ggtggcgaag ttctcgagaa tcacgatcac ctgcgcgccc gagtccttca
      421 gctggtgctc gagctcgcgc ggcgtgtaga gcggattgac gttcaccacc accccgcccg
      481 cgcgcagcgt gccgtacagg atcaccgggt actgcaggca gttcggcatc atgatcgcga
      541 cgcgcacgcc cttgccgtag cccttcgact gcagccaggc gccgaaggcg cgcgacagcg
      601 cgtcgagctc gccgaagctg atcgtcttgc ccatgctgtg gaacgccggc cgctcgcgaa
      661 actcggctac tttgtggtcc acgagatcgc ccaccgattt gaactcgtcg tagcggatgt
      721 cggcgggtac gccgggcgga tagcttttca gccagatgcg ctccatgcct cctcctaaca
      781 ggggccgctc gttttccgag ccgtcagttt taccacgagg ggcgcggcaa ggcacccggc
      841 gccgatcgcg gcgtacgcga tgacccatgc ggcacccttc gccgcgccgc ccccggcgtc
      901 gagcgcggcg ccgaagatga ccgggccgag caggccgccg ccgaatccgg cgagcgagta
      961 aaggcccatc gcggcaccgc gcagctccgc cggagccgca gcgactaggc cggcggtgag
     1021 cgttcccgac tcggccatga cgagcatgga gtatgcgatg agcagtgcca gcacgagccc
     1081 ccagtgccag ggcgcggcca gggcgaccac gaccccgctc gcgccggacc ccgccatcgc
     1141 cagcatgatc cagcgccgcc ggccgatgcg cagtgcaatc tcgttgccga cgatcgaggc
     1201 aggcacggcg agcaggttaa ccaccgccgc aatcgccggc agtcgccagg ggaaggtttc
     1261 gctgccgtgc agcgccgatg aaaacgcgag gaaggccacc atccaggcgc gcgagccgaa
     1321 gagctccaga cagtgcgcga cgtagccgag cgtatagccc gcggtggcgc ggtcggacag
     1381 gaccttgcgc caggcgacga ccggaaacag ggtattcaga gagaaaccca gagtgggccg
     1441 cggagcatga ggcagcggct cgatcagcgc gaacaccagc gatgcggcga gcagcggtcc
     1501 cgcagccgat acgatgaatg ccatgcgcca cccggcgagc ggcgcgacca ggccggcgag
     1561 cgcgagcgag agcgccgtgc cgatgccgaa gaacgaggta tagaaggcga tggagcggct
     1621 ttgccgcggc ccagcgatgc gatcggagag caaacgcagt cccggcatgt aggtggcggc
     1681 gatgccgacg ccgagcaggg cctggaacaa caccgcggct gcgaacccgt ctgcggcgag
     1741 gccgaagccc gcgctggctg ccgcggcgat gacgctgccg ccgacatata ccttgcggcc
     1801 gtcgaggcgg tcggtcagcg cggtccaata cgacaccgtg gcgacgtagc cggcgaaaaa
     1861 catgccgccg accacgccgg cctgcacgtt cgacagcgcc cactcgtcgc gcagcccggg
     1921 cagcagcgcg gcataagtcg cgaagcccag catcgaaagg atgtgggcga cgagcaggac
     1981 cggggcgggg ctaacggttc ttgaaagccg gtttgcgttt ttcgaggaaa gcggacatgc
     2041 cttccttctg gtcctcgagc gcgaactgcg aatggaacag gcgccgctcg aagcgcacgc
     2101 cctccgcgag cgagctctcg taagcgcggt tgatcgattc cttgatcatg atcaccaccg
     2161 gaagcgagca ggcggcgatc ttctgcgccg ccgcgagcgc ttctgccatc agcttgtcga
     2221 gcggcacgac gcgcgacacc agcccggcgc gctcggcttc cgcggcgtcc atcatgcgcg
     2281 ccgtcaggca caggtccatc gccttcgcct tgctggtgaa gcgcggcagg cgctgcgtgc
     2341 cgccggcgcc gggcattacg ccgagattga tctcgggctg gccgaacttc gcgttctcgg
     2401 cggcgatcag gatgtcgcat tgcatcgcga gctcgttgcc gccgcc
//
LOCUS       DASPXW010000386         4211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_743567, whole genome shotgun sequence.
ACCESSION   DASPXW010000386 DASPXW010000000
VERSION     DASPXW010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743567"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VEL04_08905"
     CDS             complement(<1..374)
                     /locus_tag="VEL04_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48527.1"
                     /translation="MNTKTVVSVAAALSLCCSAAWAQHPASASNMVLLGHQDLQGRSA
                     YQPLIHEQGGRWITYVGHHGGRVLNSLTGREEDNGTSIVDVTDPRRPRYIAHIPGEPG
                     TGETGGAQMVRVCNGKDLPKGDR"
     gene            complement(416..1369)
                     /locus_tag="VEL04_08910"
     CDS             complement(416..1369)
                     /locus_tag="VEL04_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYT48528.1"
                     /translation="MRVFLLGLLFIGQAWAQSYPSKPIRMVAGFPPGGAADFVARTLA
                     EPLGRALGQPVIVDNRPGAGSSIAAEHVAKSPPDGYTLLIGSPSSILVNPLIYPKLGY
                     QPFKDLVAVSKVTSSPLVIAVNPSLGVNSLAELIAYARRYPGKLNFASSGSGAAPHLA
                     AVLFQRLANVDMVHVPYKGGAPAVQSLLAGDTQLSFATPPSVLPLVQAGRLRALAVTS
                     RNRTPLVPGVPGMAEAGLPDYEISFWYGFFVPARTPPEVVRRIFAATEQALKLPDTGK
                     TLGREGTEASGSSSPEDFVAFLAEDAKLWARLVKDSGAKAD"
     gene            complement(1366..2163)
                     /locus_tag="VEL04_08915"
     CDS             complement(1366..2163)
                     /locus_tag="VEL04_08915"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HYT48529.1"
                     /translation="MRGKHIAVLESRLGKQLAELVAQRGGVPFHAPALAEVPDLDAGK
                     IAALVRSLEARAAKLAVFQTGVGTKALFNATDSSGLTSKLLENLGKMIVAVRGPKPTA
                     ALRARGVRIDRSAADPFTTREVLQCIQDVPLKGERVIVQRYGVANTELDHALEARGAE
                     VTEIPTYRWSLPEDTRPLAELIAKLERDEMQAVVFTNAEQARNLFLVSENLNKTEVLR
                     SALNRTLVASIGPVASAALREAGVKVGLESSPPKLGALLAALEGALS"
     gene            complement(2170..3438)
                     /locus_tag="VEL04_08920"
     CDS             complement(2170..3438)
                     /locus_tag="VEL04_08920"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HYT48530.1"
                     /translation="MDLTLQVGKYEVQKLLGKGTTGTVYLARDTFTGKEVALKTIEPE
                     VFRDPEFGTVYRSQFLQEASLAGKLRHPHIVSILDAVVGEDSGYIAMELVMGGDLSKH
                     VTPGKLLPVLDVLQIAFKCCGALEYAAKEGIVHRDIKPANIMVAQGTEVKIADFGAAV
                     LKKSQVAQTAAMGSPYYMSPEQIEGKKLNFHSDMYSLGVVMYELLTGRRPFLAENLDV
                     LMQKIIKLDPQPPSEARADLPKPLDDLVLRAMKKAPGQRYASWGEFALELSKAGQLVL
                     PPGTIPDSERYVALKSVPMLSMLADSELWELARAGRWSRVGKGKKIVKEKERGTSFYF
                     LATGQGKVTKGGRLLNMVNGGEFFGEMAYIAGGEQRHATVESMTELLLAEFDPGALDR
                     MSLGAQLQLTRALVRNVADRLELANTRLAR"
     gene            3485..>4211
                     /locus_tag="VEL04_08925"
     CDS             3485..>4211
                     /locus_tag="VEL04_08925"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48531.1"
                     /translation="MTRFDPALIQRGARLRRSPYFEATQGAGCRGYTVYNHTLLPINF
                     DAFEAEYRRLLTGVTLWDVAVERNLEIAGADAERFAELLTPRDLSKCPVGEGRYVVLT
                     DDAGGIVNDPVLLRIEAQRFWLACADSDVLLWAKGVARGAGLRVELRELDVAPLQLQG
                     PKSRAVITALFGNAFPSLKYYQFVKIELRGIPLIVTRTGWTGELGYELYLLDPLRGAE
                     LWQLVMDAGRPFGISPSGPCDIRR"
BASE COUNT          648 a         1429 c         1426 g          708 t
ORIGIN      
        1 cggtcgccct tgggcaggtc cttgccgttg cacacccgca ccatctgcgc gccgccagtc
       61 tctcccgtgc ccggctcgcc cgggatgtgc gcgatgtagc gcggcctgcg cgggtcggtg
      121 acgtcgacga tcgaggtgcc gttgtcctct tcgcgcccgg taagcgagtt gaggacgcgg
      181 ccgccgtggt gcccgacata ggtgatccag cggccgccct gctcgtggat cagcggctgg
      241 taggcgctgc gcccctgcag gtcctggtgg ccgagcaaga ccatgttcga agcgctggcg
      301 ggatgctgcg cccaggccgc gctgcagcat agcgaaaggg cagccgccac ggacactacg
      361 gttttcgtat tcatcggata ttctccctgc gaggaaggca atcctacccg aacggtcagt
      421 cggctttcgc gcccgagtcc ttcaccaggc gcgcccagag cttcgcgtcc tcggcgagaa
      481 aggcaacgaa gtcctcgggg gacgaggatc ccgaggcctc ggtgccttcg cgtcccagcg
      541 tcttgccggt atccggcagc ttcagcgcct gctcggtcgc cgcgaagatc ctgcgcacca
      601 cctcgggcgg agtgcgggcg ggaacgaaaa acccgtacca gaacgagatc tcgtagtcgg
      661 gcaggccggc ctccgccatt cccggcacgc cgggcacgag cggcgtgcgg ttgcggctgg
      721 tgaccgcaag cgcgcgcaag cgacccgcct gcaccagcgg cagcaccgaa ggcggcgtgg
      781 cgaacgaaag ctgcgtgtcg cctgcgagca gcgactgcac cgccggcgcc ccgcccttgt
      841 acggcacgtg caccatgtcg acgttggcga ggcgctggaa cagcactgcg gcgaggtgcg
      901 gcgcagcgcc gctaccggag gaggcgaagt tgagcttgcc cggatacctg cgcgcgtacg
      961 cgatgagctc cgccagggag ttgactccca ggctcgggtt taccgcgatc accagcggcg
     1021 atgaggtgac tttgctcacc gccaccagat ccttgaacgg ctggtagccg agcttggggt
     1081 agatgagcgg attgacgaga atgctcgacg ggctgccgat cagcagcgtg tagccatcgg
     1141 gagggctctt cgccacgtgc tccgccgcga tgctcgagcc ggcgccgggc cggttgtcga
     1201 ctatgaccgg ctggccgagc gcgcgcccga ggggctcggc gagcgtgcgc gcgacgaagt
     1261 cggccgcgcc gcccggcgga aagccggcga ccattctgat gggcttcgaa ggatacgact
     1321 gcgcccacgc ctgcccgatg aaaagcaatc caaggaggaa aactctcatg aaagagcgcc
     1381 ctccagcgct gcgagcagcg cgccgagctt gggcgggctc gattcgagcc cgaccttgac
     1441 cccggcctcg cgcagcgcgg cggaggcgac cgggccgatc gacgccacca gcgtgcggtt
     1501 gagcgcgctt ctcagcactt cggttttatt caggttctcg gaaacgagaa acaggttgcg
     1561 cgcctgctcg gcgttggtga acaccaccgc ctgcatttca tcgcgctcga gcttcgcgat
     1621 cagctcggcg agcggccgcg tgtcctcggg aagcgaccag cggtaggtcg ggatctcggt
     1681 cacctcggcg ccgcgcgcct cgagcgcgtg atcgagctcg gtattggcga cgccgtagcg
     1741 ctgcacaatg acgcgctcgc ccttcagggg aacatcctgg atacattgca ggacttcgcg
     1801 ggtggtaaac ggatctgcgg cactgcggtc gatgcgcacg ccgcgcgcgc gcagcgccgc
     1861 cgtgggcttc gggccgcgga ccgcaacgat catcttgccg aggttttcga ggagctttga
     1921 ggtcaacccc gacgaatcgg tcgcgttgaa caacgccttc gtgccgaccc cggtctggaa
     1981 caccgcgagc ttcgccgccc gcgcctccag cgagcgcacc agcgcggcga tttttccggc
     2041 atcgaggtcg ggcacctcgg cgagcgccgg ggcatggaag ggcacgccgc cacgctgcgc
     2101 gacgagctcg gcgagctgct tgccgagccg gctttcgagc acggcaatgt gcttgccccg
     2161 catggagcgc tagcgcgcga ggcgcgtatt ggcgagctcg aggcggtcgg cgacgttgcg
     2221 caccagcgcg cgggtgagct ggagctgcgc gccgaggctc atcctgtcga gcgcccccgg
     2281 gtcgaactcg gcgagcagca gctcggtcat cgattcgacc gtggcgtggc gctgctcgcc
     2341 gcccgcgatg taggccatct cgccgaagaa ttcgccgccg ttgaccatgt tgagcagccg
     2401 gccgcccttg gtgaccttgc cctggccggt ggcgaggaag tagaagctgg tgccgcgctc
     2461 cttctccttg acgatcttct tgccctttcc cacgcgcgac cagcggccgg cgcgcgcgag
     2521 ctcccacagc tcggaatccg ccagcatcga gagcatcggc acgcttttca gcgccacgta
     2581 gcgctcgctg tcgggaatcg tgccgggcgg cagcaccagc tgcccggcct tcgacagctc
     2641 gagggcgaac tcgccccagc tcgcgtagcg ctggcccggg gccttcttca tggcgcgcag
     2701 caccaggtcg tcgagcggct tcggcaggtc ggcgcgcgcc tcgctcggcg gctgcggatc
     2761 gagcttgatg atcttctgca tcagcacatc gaggttctcg gcgaggaacg ggcgcctgcc
     2821 ggtgagcagc tcgtacatca ccacgccgag cgagtacatg tcgctgtgga agttgagctt
     2881 cttgccctcg atctgctccg gcgacatgta atagggcgag cccatggcgg cggtctgcgc
     2941 cacctggctc ttcttcagca ccgcggcgcc gaagtcggcg atcttcacct cggtgccctg
     3001 cgccaccatg atgttcgccg gcttgatgtc gcggtgcacg atgccttcct tcgccgcgta
     3061 ttcgagcgcg ccgcagcact tgaaggcgat ctgcagcacg tcgagcaccg gcagcagctt
     3121 gccgggggtg acgtgcttcg acaggtcgcc gcccatcacc agctccatcg cgatgtagcc
     3181 ggaatcctcg ccgaccacgg cgtcgaggat cgagacgatg tggggatggc gcagcttgcc
     3241 ggcgagcgaa gcctcctgca ggaattgcga gcggtagacg gtgccgaact cggggtcgcg
     3301 gaacacctcg ggctcgatgg tcttcagcgc cacctccttc ccggtgaagg tgtcgcgcgc
     3361 gaggtacacg gttccggtcg tgcctttgcc gagcagcttc tgcacttcgt acttgcctac
     3421 ctggagagtc agatccattt gcgccagaat gtacctcaac tccgagcccg cctgacaaag
     3481 aaccatgaca cggttcgatc ccgccctgat acagcgcggt gcgcgtctgc gccgctcacc
     3541 ctatttcgag gccacgcagg gcgccggctg ccgcggctac acggtgtaca accacacgct
     3601 gctgcccatc aacttcgacg ctttcgaggc cgagtaccgc cgcctcctga ccggcgtgac
     3661 gctgtgggac gtggcggtcg agcgcaacct cgagatcgcc ggcgccgacg cggagcgctt
     3721 cgccgagctg ctgacgccgc gcgatctctc gaaatgcccg gtcggcgagg gccgctacgt
     3781 ggtgctcact gacgatgcgg gcggcatcgt gaacgatccg gtcctgctgc gcatcgaggc
     3841 gcagcgcttc tggctcgcct gcgccgacag cgacgtgctg ctctgggcga agggcgtggc
     3901 gcgcggcgcg ggcctgcgcg tcgagctgcg cgagctcgac gtggcgccgc tccagctcca
     3961 gggacccaaa tcgagggctg tcatcactgc gttattcggc aatgcctttc cgtcgttgaa
     4021 gtattaccag ttcgtgaaaa tcgagctgcg cggtattccg ctgatcgtca cgcgcaccgg
     4081 ctggaccggg gagctcggct acgagctcta cctgctcgat ccgctgcgcg gcgcggagct
     4141 ctggcagctc gtcatggatg ccgggcggcc gttcggcatc tcgccgtccg gcccctgcga
     4201 catccgccgc a
//
LOCUS       DASPXW010000387         2857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1625297, whole genome shotgun sequence.
ACCESSION   DASPXW010000387 DASPXW010000000
VERSION     DASPXW010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1625297"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VEL04_08930"
     CDS             <1..221
                     /locus_tag="VEL04_08930"
                     /inference="COORDINATES: protein motif:HMM:NF023363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3455 domain-containing protein"
                     /protein_id="HYT48532.1"
                     /translation="KAVANVAGQTPGDIPWLKLEVTAHRGSGTLSGVTTVQRINTKGG
                     AHTGACDKAGALHSAPYAADYVFLRKGS"
     gene            complement(351..>2857)
                     /gene="alaS"
                     /locus_tag="VEL04_08935"
     CDS             complement(351..>2857)
                     /gene="alaS"
                     /locus_tag="VEL04_08935"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HYT48533.1"
                     /translation="TGLEKRPYTRAVTAQKCVRAGGKHNDLDNVGYTARHHTFFEMLG
                     NFSFGDYFKDRAIELAWNLITKEFGLPVDRLLVTVFSEDDQAFDLWRKIAGLPESKIV
                     RIAGSDNFWQMGELGPCGPCSEIFYDHGPHIPGGPPGSSEAEGDRFIEIWNLVFMQFE
                     QLPGGKRVDLPKPSIDTGMGLERVAAVLQGTHDNYKIDLFGALISHVADLTGVEPEGE
                     RAVSHRVIADHLRASSFLIADGVLPSNEGRGYVLRRIMRRAMRHAQLLGADEPVMWRL
                     VPTLVREMGQAYPELSRAEPLITETLKLEETRFRTTLARGLALLEEGSKGLARGAKFS
                     GEAAFTLYDTYGFPLDLTQDALKPRGITVDTESFGAAMQRQREKARASWAGSGEAATE
                     TLWFPIKERVSATEFLGYETETAEGVIAALVKDGKEVEALRAGESGAVVLNQTPFYGE
                     AGGQVGDTGTMHGDGVRLSVTDTQRKAGDVLVHSGKVEKGTLKIGAALTLEVDHSRRS
                     AIRRNHSATHILHEALRQVLGDHVAQKGSLVAPDRLRFDFSHPKPMTAEEIERVEDIA
                     NEVVLQNSPVTTRLMAVDEAIASGARALFGEKYGDEVRVVAMGEGGGNTMGWSVELCG
                     GTHVKRTGDIGLIAALGDSAVAAGVRRIEALTGTTARKHARHLGELAKAASAELKAPL
                     EEVPQRLAQVLDERRRLERELSEAKKKLAMGGGSAQGADGVRVVGDVKLLARAVAGID
                     LKDLRSLADEGKKRLGSGVVAIVGVTEEGKAGVVVGVTADLTERFSAIDLVRKGAEVL
                     GGKGGGGRADMAQAGGPDGSKAEAALKAIEGALGGS"
BASE COUNT          442 a          980 c          956 g          479 t
ORIGIN      
        1 caaaggcggt cgccaatgtc gcaggccaga cgccgggtga catcccctgg ctcaagctcg
       61 aggtgacggc gcatcgcggc agcggcacgc tatcgggcgt caccacggtg cagcggatca
      121 acacaaaggg cggtgcgcat accggcgcct gcgacaaagc cggcgccctg cacagcgcgc
      181 cctacgccgc ggattacgtc ttcctgcgca agggctcgtg agcgagtcgg cgcgacaaag
      241 cgtagggtgg gcaaaatcgc gcggttctat tgtgaggtgg gcacggcgcg tgcgcaattt
      301 tgcccacgcg gaaacaccga acagcgcgcc tttgcccacc ctacgcacag ctaggaaccg
      361 ccgagtgcgc cctcgatcgc tttcagcgcc gcctcggcct tcgacccgtc ggggccgccg
      421 gcctgcgcca tgtccgcgcg cccgcccccg cctttgccgc cgagcacctc ggcgcctttg
      481 cgcacgaggt cgatcgcgga gaagcgctcg gtcaagtcgg cggtaacgcc gaccacgacg
      541 ccggccttac cttcctcggt gacgccgacg atggcgacga cgcccgagcc gagtcgcttc
      601 ttgccttcgt cggcgaggct gcgcaggtcc ttgagatcga tgcctgcgac cgcgcgcgcc
      661 aaaagcttca cgtcgccgac gacgcgcacg ccgtcggcgc cttgcgcgct gccgccgccc
      721 atggcgagct ttttcttcgc ctccgacagc tcgcgttcga ggcggcgccg ctcatcgagc
      781 acttgcgcga gacgctgcgg cacctcctcg agcggcgcct tgagctccgc ggatgcggcc
      841 ttggcgagtt cgcccaaatg ccgcgcgtgc ttgcgcgccg tggtgccggt gagcgcctcg
      901 atgcggcgca cgccggccgc gaccgcgctg tcgccgagcg cggcgatgag gccgatgtcg
      961 ccggtgcgct tgacgtgcgt gccgccgcag agctcgaccg accagcccat ggtgttgccg
     1021 ccgccctccc ccatggccac gacccgcacc tcgtcgccgt atttctcgcc gaacagcgcg
     1081 cgcgcgcctg acgcaatcgc ttcatcgacc gccatcagtc gcgtggtcac cggcgagttc
     1141 tgcagcacga cctcgttggc gatgtcctcg acccgctcga tctcctccgc cgtcatcggc
     1201 ttgggatggg aaaagtcgaa gcgcagccgg tcgggcgcga ccaacgagcc cttctgcgcg
     1261 acgtgatcgc cgagcacctg gcgcagcgcc tcgtgcagaa tgtgcgtcgc cgaatggttg
     1321 cggcgaatgg cgctgcgccg ggaatgatcg acctcgaggg tgagcgcggc accgattttg
     1381 agcgtgcctt tttccacctt gccggagtgc acgagcacgt cgcccgcctt gcgttgggtg
     1441 tcggtgacgg acaaacgcac gccgtcgccg tgcatggtgc cggtgtctcc gacctgcccg
     1501 cctgcttcgc cgtaaaacgg tgtctggttg agcacgaccg cgccgctctc gccggcgcgc
     1561 aaggcctcga cctccttgcc gtccttgacc agcgcggcga tcacgccctc ggcggtttcg
     1621 gtctcgtagc cgaggaactc ggtggcgctg acgcgctcct tgatggggaa ccacaatgtt
     1681 tcggtggccg cctcgcccga tcccgcccag gacgcgcgcg ccttttcgcg ctggcgttgc
     1741 atggccgcgc cgaaactttc ggtatcgaca gtgatcccgc gcggcttgag cgcatcctgg
     1801 gtcagatcga gcggaaagcc gtaggtgtcg tagagcgtga aagcggcttc gccggaaaat
     1861 ttcgccccgc gcgcaagccc cttgctgcct tcctcgagca gcgcaagccc gcgcgcgagc
     1921 gtcgtgcgaa accgcgtctc ttcgagcttg agcgtctccg tgatgagtgg ctccgcgcgc
     1981 gaaagctcgg gataagcctg gcccatctcg cgcaccagcg tggggacgag gcgccacatc
     2041 accggttcgt cggcgccgag aagctgcgca tgccgcatgg cgcggcgcat gatgcggcgg
     2101 agcacgtagc cgcggccctc gttcgagggc agcacgccgt cggcgatcag gaacgaggac
     2161 gcgcgcaggt ggtctgcgat cacgcggtgc gacacagcgc gctccccttc cggctcgacg
     2221 ccggtcagat cggcgacgtg ggagatgagc gcaccgaaca ggtcgatctt gtagttgtcg
     2281 tgggtgcctt gcagcaccgc ggcgacgcgc tcgagcccca tgccggtgtc gatcgagggc
     2341 ttcggcagat cgacgcgctt gccgccgggc agctgctcga actgcatgaa cacgaggttc
     2401 cagatctcga tgaagcggtc gccttcggcc tcggacgagc ccggcggacc gcccgggatg
     2461 tgcgggccat ggtcgtagaa gatttccgag cagggaccgc aggggccgag ctcacccatc
     2521 tgccagaagt tgtcggagcc ggcgatgcgc acgatcttgc tctcgggcag cccggcgatc
     2581 tttctccaca gatcgaaggc ctggtcgtcc tcggagaaaa cggtgaccag caggcgatcg
     2641 accggcaggc cgaattcctt ggtgatgaga ttccaggcaa gctcgatggc gcgatccttg
     2701 aagtaatcgc cgaaggagaa attgccgagc atctcgaaga atgtgtgatg gcgcgcggta
     2761 taaccgacgt tgtcgaggtc gttgtgcttg ccgccggcgc gcacgcattt ctgcgcggtg
     2821 accgcgcgcg tatagggacg cttctcgagg ccggtaa
//
LOCUS       DASPXW010000388         2184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1751696, whole genome shotgun sequence.
ACCESSION   DASPXW010000388 DASPXW010000000
VERSION     DASPXW010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1751696"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VEL04_08940"
     CDS             complement(<1..287)
                     /locus_tag="VEL04_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48534.1"
                     /translation="MRFLAGLPLGLGAALMLGSAAACAAAQVDVFTPQGEAKGVRQVA
                     ARFTEPMVAFGDPRLPDPFDVRCEGDAAHLKGRGRWADARNWVYDFEQDLPA"
     gene            472..699
                     /locus_tag="VEL04_08945"
     CDS             472..699
                     /locus_tag="VEL04_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48535.1"
                     /translation="MTATATALTAAPVAMAAAHVNAKSRKNCRVVTLSLGFMTPHPFG
                     YGAIGAGDSGAAVVKSWHAADQSWQKDGNAR"
     gene            750..1439
                     /gene="pdsR"
                     /locus_tag="VEL04_08950"
     CDS             750..1439
                     /gene="pdsR"
                     /locus_tag="VEL04_08950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteobacterial dedicated sortase system
                     response regulator"
                     /protein_id="HYT48536.1"
                     /translation="MGRIVAIVEDDPGIRANYADMLKKHGYQVRAYSDRRSAADAFRT
                     QLPDLALLDIGLGDEPDGGFTLCRELRAMSAALPIIFLTARDSDFDTVSGLRIGADDY
                     LTKDVGLPHLLARISALFRRADLLAQPPATEDVLERVPLRLDMKRFQASWHGNTVDLT
                     LTEFWLVHSLARFPGHVKDREQLMRDAHIVVDDSTITSHIKRIRRKFQAVDREFGAID
                     SVYGLGYRWNA"
     gene            1447..>2184
                     /locus_tag="VEL04_08955"
     CDS             1447..>2184
                     /locus_tag="VEL04_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48537.1"
                     /translation="MRLSLRAKVVLVALVLSLIPWVGYLHLTQMDALLRQGQEQALLA
                     TARAVATALHDRPELLELRPGAPASGEMELILKSLARAESRIWIVDQRQRLLALAGDL
                     KKRAPGEERGAAAPAPLDFAMRMVRPVTDFLLERPTEDFDDALSEAEIAHGPVAASAL
                     QGVPARRWRHSPDSRAVILSASHPVWAGEQVVAAVVAEETTNAIRSIYNRALEQVVAV
                     TLIAFLAGALTLILFASRLSMRLRRLRD"
BASE COUNT          332 a          765 c          739 g          348 t
ORIGIN      
        1 gcgggcagat cctgttcgaa gtcgtagacc cagttgcgtg cgtcggccca gcggcccctg
       61 cccttaaggt gcgccgcgtc gccctcgcag cgcacgtcga aggggtccgg caggcgcgga
      121 tcgccgaaag ccaccatcgg ctcggtgaag cgcgcagcga cctgcctcac gcctttcgcc
      181 tcgccctgcg gcgtgaacac gtccacttgt gcagccgcgc aggcggcggc cgatccaagc
      241 atgagcgccg cgcctaggcc gagcggcaga ccggccagaa acctcattcg ctccctcccc
      301 tgtttcgtaa gtgcagatta gacgccgcgg cggcgctggg ggctaggggc tggggaccgg
      361 cgtgcagggc gcacgccgcg tcgaggcggg gaggaccaag caaagcccca gccaccagcc
      421 cgcggcgccg ccgcggccgg cgttcatccc ccgtgcacac cgaagagcaa gatgaccgcg
      481 accgcgacgg cgctgaccgc ggcgccggtc gcgatggcgg ctgcgcacgt gaacgcgaag
      541 tcgcgcaaga actgccgcgt ggttacgttg agtctcggtt tcatgacccc ccacccgttt
      601 gggtacggcg ccattggagc aggcgattcc ggcgctgcag tggtgaagtc atggcacgcc
      661 gccgatcaat catggcaaaa agatggcaat gcgcgctagc catgcgcgcg cggctcgtgc
      721 gcctggacca gtccggtata ttgccgccca tgggacggat cgtggcgatc gtcgaggatg
      781 atcctggcat tcgcgcgaac tacgccgaca tgctgaagaa gcacggctac caggtgcgcg
      841 cctactctga ccgcagatcc gccgcggatg cgtttcgcac gcagttgccg gatctcgcgc
      901 tgctcgacat cggtctgggc gacgagcccg acggcggctt cacgctgtgc cgcgagctgc
      961 gcgccatgtc ggccgcgttg ccgatcatct tcctcaccgc gcgcgacagc gatttcgaca
     1021 cggtgtccgg attacgcatc ggcgccgacg actacctgac gaaagacgtg ggattgcctc
     1081 atctcctcgc gcgcatcagc gcgctcttcc ggcgcgccga cctgctcgcg cagccgccgg
     1141 cgacggagga cgtgctggag cgtgtgccgc tcagactcga catgaagcgc ttccaggcct
     1201 cgtggcacgg aaacaccgtg gacctcacgc tgaccgagtt ctggctggtg cattcgctcg
     1261 cgcgctttcc gggccacgtc aaggaccggg agcagctgat gcgcgacgcg cacatcgtcg
     1321 tcgacgactc gaccatcacg tcgcacatca agcgcatccg gcgcaagttt caggccgtcg
     1381 accgggagtt cggggcgatc gattcggtct acggcctcgg ctaccgctgg aacgcttgag
     1441 gcgggcatgc gcctgagctt gcgcgccaag gtggtgctgg tggcgctggt gctgtcgctc
     1501 attccgtggg tcggttacct gcatctcact cagatggacg cgctgctgcg ccaagggcag
     1561 gagcaggcgt tgctcgcgac cgcgcgcgcg gttgccacgg cgctgcacga tcgtcccgag
     1621 ctgctcgagc tgcggcccgg cgcgccggcc tccggcgaaa tggagctcat cctcaagagc
     1681 ttggcgcgcg ccgagtcgcg catctggatc gtagaccagc gccagcgcct gctcgcactc
     1741 gccggcgacc tgaagaaacg cgctcctgga gaggagcgcg gcgcagccgc gccagcgccg
     1801 ctcgacttcg caatgcgcat ggtccggccg gtcaccgatt tcctcctcga gcggccgacc
     1861 gaggacttcg acgatgcgct ctcggaagcc gagatcgccc acggccccgt ggcggcgagc
     1921 gccctgcagg gtgtgccggc gcggcgctgg cggcactccc ccgactcgcg cgcggtcatc
     1981 ctctcggcct cgcatcccgt gtgggcggga gagcaggtcg tcgccgccgt ggtggcggag
     2041 gagaccacca acgccatccg ctcgatctac aaccgggcgc tcgagcaggt cgtcgcggtg
     2101 acgctgatcg cgtttctcgc tggtgcgctg acgctgatcc tcttcgcctc gcgcctgtcc
     2161 atgcggctca ggcgccttcg cgac
//
LOCUS       DASPXW010000389         5696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1751714, whole genome shotgun sequence.
ACCESSION   DASPXW010000389 DASPXW010000000
VERSION     DASPXW010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5696
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1751714"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1655)
                     /locus_tag="VEL04_08960"
     CDS             complement(<1..1655)
                     /locus_tag="VEL04_08960"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HYT48538.1"
                     /translation="MHREKGSRAYRTAPTDATTFYPRLIGRHGAVAANSYLSVNAGVD
                     VLKAGGNAIDAAVAASFVEGLVNPQMHTIGGECPILIRAAQSDKVVALNGNMAAPARA
                     TPEEFRHRGLDQVPDEDILAAGAPAAFGALATALSRFGSLPLSEVIAPAIDLAKNGFP
                     VSQGLRNQERYGLKHLQEKFQHWPGSKGIYLPLPEIGNLLKNEPLARTYDYLANAKDP
                     LAAFYKGEVAAEIARFSKERDGLLERADLEAFQTRLEEPAKFNFGEATVYKCGFWSQG
                     PAMLQALAILKCFDLKNLSPASADYLHFLIEATKLAFADREQYYGDPSQVMMPAEVLL
                     SEAYGKERSKLIDQKKANDELRPGDAWRNAALLPADERFTPQPWDPGTVHVDAIDARG
                     NMASFTPSGAWLKSAPVIEALGFPLSTRMMTFYLGPPSHPNVVAPFKRPRTTLTPSLA
                     FRRGRPWMTFGTMGGDQQEQWSLQFILYREAFGMTLQEAIEAPKLSSDHFPGFFAPHD
                     HFPKRVRIEARFGAKAIDELRRRGHDIEVAPDWTEGFLSAAQI"
     gene            complement(1732..2208)
                     /locus_tag="VEL04_08965"
     CDS             complement(1732..2208)
                     /locus_tag="VEL04_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48539.1"
                     /translation="MRIAFALVIISLSLGSGGVLAAGDPGSSSRMETPKDPVLEKVAA
                     ATSRQDWAGAQSLLQDALSKDAGNADYHNLYAYSIRKGPNPDMSLVFKHYNEALRIDP
                     KHRGAHEYLGEAYLMVGNVAKAKEQLGALDRLCFFGCSEFTDLKKAIAEHEKQATK"
     gene            2320..2868
                     /locus_tag="VEL04_08970"
     CDS             2320..2868
                     /locus_tag="VEL04_08970"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYT48540.1"
                     /translation="MRSADAATRAEAIVWLANRGDMADAKLLHQRLRDESAFVRSFAE
                     QGLWLLWSRSGDAGIDGLMARGAEEMQAGHYPEAISVFTQVIQKKPAFAEGWNRRATT
                     YYLAGEYARSLADCDEVLKRNPGHFGALSGVGQIYFRLEEYEQALAWFRRALEINPNM
                     LGVEINIKGIEELLKSRRRHAT"
     gene            complement(2872..3273)
                     /locus_tag="VEL04_08975"
     CDS             complement(2872..3273)
                     /locus_tag="VEL04_08975"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HYT48541.1"
                     /translation="MDLIAHFRGARNVVSVPSGLVLFRAGEPGANMYVLIKGMAEIYI
                     GEEVIELATAGALLGEMAMVDSTVRSATVITRTGCRLVSIDVSQFDLLVRESPEFARH
                     VMTVIAGRLRHMNERLKEALGELSVRGRRPK"
     gene            complement(3286..>5696)
                     /locus_tag="VEL04_08980"
     CDS             complement(3286..>5696)
                     /locus_tag="VEL04_08980"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HYT48542.1"
                     /translation="RSRKVIRCNRRFEEMFGFGTGEALGASWREMYFTDEEFERRGQT
                     YGELDAGRTHARESWVRKQDGSGFWCRISARAVNAGEPSKGYVFLFEDISERKRADEV
                     LERVVREQDAVLQNALAGIIFVKERRIVRCNRRFEEIFGYAEGELLNRTTRFMFASDE
                     DFTAGGDRVYEMVWAGETQYLERTHVRKDGTQIWCSISGRAVQPGDPSEGSVWLFDDI
                     TQEHAAEERVQRALAEQELILDNATVGIAFVRNRAIQRCNRFLEDMVGAGPGELVGQT
                     SGTLFASKQEWKKAREAAYAATAPGETHEAEHRLRRRDGTTFLCRMRGRRIDAGEPQQ
                     EWIWSYEDVTAEREADLRVKRALDELERAVAERTAELQEAKAHAQHLAAHDALTGLPN
                     RRLLEDRLTQALALSQRNRQQTAVMFVDLDRFKHINDSFGHAAGDVLLKEVSQRLVNQ
                     LRTGDTICRIGGDEFVVVLPEVKRSSDVAQVAQKVIEQLSQPVSIEERELIVTPSIGI
                     AVFPEDGRDAETLIRNADAAMYHAKELGRANYQFFTDQMNQAASRRIALESDLRRALG
                     RGELRVHYQRIVGADGRGAGHEALVRWQHPERGLVMPADFLQLAEETGLILRIGEWVL
                     REACRWGTFIGTERGLQVAVNLSPRQFSDPNLPAVVARALKDTGLPPSLLELEITESL
                     AMQQTDATLATLKKLKRLGVSIAIDDFGTGYSSLAYLKRFPVDKLKVDRSFVAGLPQD
                     RDQAAIVRAIVALAHALQIRVIAEGVETDAQRAFLASCGCDFIQGHLVGRPLDADIAA
                     KEYV"
BASE COUNT          913 a         1938 c         1857 g          988 t
ORIGIN      
        1 tcgatctgcg cggcggagag aaagccttct gtccagtcgg gggccacctc gatatcgtgg
       61 ccgcgcctgc gcagctcgtc aatcgccttg gcgccgaagc gcgcctcgat tctcacccgc
      121 ttcgggaaat ggtcatgcgg cgcaaagaag cccgggaagt ggtccgagga aagcttcggc
      181 gcctcgatcg cctcctggag cgtcatgccg aacgcctcgc gatacaggat gaactgcagc
      241 gaccattgct cctgctggtc gccgcccatg gtgccgaagg tcatccacgg cctgccgcgc
      301 cggaacgcga gcgaaggcgt gagggtggtg cgcggccgct tgaacggcgc caccacgttc
      361 gggtggctcg gcggcccgag gtagaaggtc atcatccgag tcgaaagcgg gaaaccgagc
      421 gcctcgatca ccggcgccga cttgagccac gcgccgctcg gcgtgaagga ggccatgttg
      481 cccctggcgt cgatcgcgtc gacatgcacc gtgcccgggt cccagggctg cggcgtgaag
      541 cgctcgtcgg ccggcaggag cgcggcattg cgccacgcgt cgccgggacg cagctcgtcg
      601 ttcgccttct tctgatctat taatttgctt cgttccttcc cgtaagcttc cgagagcagc
      661 acttcagcag gcatcatcac ctgtgaggga tcgccgtagt actgctcgcg gtcggcgaac
      721 gcgagcttcg tcgcctcgat gagaaaatgc aggtagtcgg cgcttgcggg acttagattt
      781 ttcaagtcaa aacatttcag aatggccagc gcctgcaaca tggccgggcc ctgagaccag
      841 aagccgcatt tgtagacagt cgcctcgcca aaattgaatt ttgcgggttc ttcaaggcga
      901 gtctggaagg cttcgagatc cgcgcgctcg agcagcccat cgcgctcctt cgaaaagcgc
      961 gcgatctcgg ccgcgacctc gcccttgtag aaagcggcga gcgggtcctt ggcgttcgcc
     1021 aggtagtcat aagtcctcgc gagcggttca ttttttaaaa gattgccaat ttcaggtaac
     1081 ggaagataaa tgcctttgct ccccggccag tgctgaaact tctcctgcag gtgcttgagg
     1141 ccatacctct cctggttgcg caggccctgc gacaccggga agccgttttt cgcgagatcg
     1201 atcgccggcg cgattacttc cgacaacggc aggctgccga agcgggaaag cgcggtggcg
     1261 agcgcgccga acgccgcagg cgcgccggcg gcgaggatgt cctcgtcggg aacctgatcg
     1321 aggccgcggt ggcggaactc ttccggcgtc gcgcgcgccg gcgcggccat gttgccgttc
     1381 agtgccacta ccttgtcgct ctgcgcagcg cggatcagga tcgggcattc gccgccgatg
     1441 gtatgcatct gcggattcac cagcccttcg acgaagctcg ccgcgaccgc ggcatcgatc
     1501 gcgttgccgc ccgccttcaa cacgtcgacc ccggcgttga ccgagagata cgagttggcc
     1561 gccaccgcgc cgtgcctgcc gatgaggcgc ggatagaacg tcgtcgcgtc ggtgggcgcg
     1621 gtgcgatagg cgcgcgaacc tttctcgcgg tgcatgtcgc tagaatagcc gacgccgacc
     1681 gcttgacccc ggaacacccg ggcggtcagg aagctggcgc tgctgcggct gttacttcgt
     1741 cgcctgcttc tcgtgctcgg cgatcgcctt cttgaggtcg gtgaactcgc tgcagccgaa
     1801 gaagcacagc ctgtcgagcg cgccaagctg ttccttggcc tttgccacgt tgccgaccat
     1861 caggtaagcc tcgccgaggt actcgtgcgc gccgcggtgc ttgggatcaa tccgcagcgc
     1921 ctcgttgtag tgcttgaaca ccaggctcat gtccgggttc gggcccttgc ggatcgagta
     1981 ggcgtagagg ttgtggtagt cggcgttgcc ggcatccttc gagagcgcgt cctgcagcag
     2041 gctctgggcg ccagcccagt cctggcgcga ggtcgcggcg gcgaccttct cgagcaccgg
     2101 gtccttcggc gtctccatcc tgctcgagct gccagggtcg cctgcggcaa gcacgccgcc
     2161 cgagccgagc gaaagcgata tgatgaccag cgcgaaagca attctcatat gttgtccctt
     2221 tccagactgc gaatactact ccttgcctgt gcgttaacag cggccgctgc gccggcattc
     2281 cccgagccgc cgaaatcgcg cgatcaggcg ctcgccagct tgcgcagcgc tgacgccgcg
     2341 acgcgcgccg aggcgatcgt gtggctcgcg aaccgcggcg acatggccga tgcgaagctc
     2401 ctgcaccagc ggctgcgcga cgaaagcgcg ttcgtgcgca gctttgccga gcaggggctg
     2461 tggctgctct ggagccgctc cggcgacgcc gggatcgacg gcctgatggc gcgcggcgca
     2521 gaagaaatgc aggcgggcca ctaccccgag gcaatcagtg tttttacgca agtgattcaa
     2581 aaaaaacccg cattcgcgga gggctggaac cggcgcgcga ccacctatta cctcgccggc
     2641 gagtacgcca ggtcgctcgc cgactgcgac gaggtgctca agcgcaaccc cgggcacttc
     2701 ggcgcgctct cgggcgtggg gcagatctac ttcaggctcg aggaatacga gcaggcgctc
     2761 gcctggttca ggcgcgcgct cgagatcaat ccgaacatgc tcggcgtcga gatcaacatc
     2821 aagggcatcg aggagctgct gaagtcaagg cggcgtcacg ccacctagcg ctcacttcgg
     2881 ccgtcgacca cgaaccgaca actcgccgag cgcctcttta agcctttcgt tcatgtgcct
     2941 tagacggccg gcaatcaccg tcatgacgtg cctcgcgaac tccggagact cgcgtaccag
     3001 cagatcgaac tgtgaaacgt cgatcgaaac cagccggcaa cccgtccggg taatcaccgt
     3061 ggcgcttcgc acggtcgaat cgaccatggc catttcccca agcagcgccc ccgcggtggc
     3121 taactcgatg acctcctcgc cgatataaat ctctgccatt ccctttatca gcacgtacat
     3181 gttcgcaccg ggctcgccgg cgcggaacag caccaggccc gatggcacgg acaccacatt
     3241 acgagcccct cggaagtgtg cgatgagatc catggccgcc agcccctaca cgtattcctt
     3301 cgccgcgata tcggcgtcga gcggccggcc gacgaggtgg ccctggatga aatcgcagcc
     3361 gcagctcgcg aggaaggcgc gctgcgcgtc ggtctcgacg ccttcggcga tgacgcggat
     3421 ctgcagcgca tgcgccagcg ccacgatggc gcggacgatc gccgcctggt cgcggtcctg
     3481 cggcagcccg gcgacgaagc tgcggtcgac cttcagcttg tcgaccggga agcgcttgag
     3541 gtacgcgagg ctcgagtagc cggtgccgaa gtcgtcgatc gcgatcgaca cgccgaggcg
     3601 cttcagcttc ttcagcgtcg cgagcgtggc gtcggtctgc tgcatggcga gcgactcggt
     3661 gatctcgagc tcgagcaggc tgggcggcaa gccggtgtcc ttaagcgcgc gcgccaccac
     3721 cgcgggcagg ttcgggtcgc tgaactgccg cggcgagagg ttcaccgcga cctgcaggcc
     3781 gcgctcggta ccgatgaagg tcccccagcg gcaggcctcg cgcagcaccc actcgccgat
     3841 ccgcagtatc agcccggtct cctcggcgag ctggaggaag tccgcgggca tcaccaggcc
     3901 gcgctccggg tgctgccagc gcaccagcgc ctcgtgcccg gcgccgcgcc cgtcggcccc
     3961 aacgatgcgc tggtaatgca cgcgcaactc gccccggcct agcgcacgcc gcaggtcgct
     4021 ctcgagggca atgcggcgcg aggcggcctg gttcatctgg tcggtgaaga actggtagtt
     4081 ggcgcgcccc agctccttgg cgtggtacat cgcggcgtcg gcgttgcgga tcagcgtctc
     4141 ggcgtcgcgc ccgtcctcgg ggaacaccgc gatgccgatc gagggcgtga cgatcagctc
     4201 gcgctcctcg atgctcaccg gctgcgaaag ctgctcgatc accttctgcg cgacctgcgc
     4261 gacatccgag gatcgcttca cctcgggcag caccaccacg aactcgtcgc cgccgatgcg
     4321 gcagatggtg tcgccggtgc gcagctggtt caccaggcgc tgcgagacct ccttcagcag
     4381 cacgtcgccg gccgcgtggc cgaacgagtc gttgatgtgc ttgaagcggt cgaggtcgac
     4441 gaacatcacc gcggtctgct ggcggttgcg ctgcgacagc gcgagcgcct gggtgagccg
     4501 gtcttccagc agcctgcggt tcggcagccc ggtgagcgcg tcgtgagcgg cgaggtgctg
     4561 cgcgtgcgcc ttggcctcct gcagctcggc ggtgcgctcc gcgaccgcgc gctcgagctc
     4621 gtcgagcgcg cgcttcacgc gcaggtcggc ctcgcgctcg gccgtgacgt cttcgtagct
     4681 ccagatccat tcctgctgcg gctcgccggc gtcgatgcgg cgcccgcgca tgcggcacag
     4741 gaaagtggtg ccatccctgc gccggaggcg gtgctcggcc tcgtgggtct cgccgggcgc
     4801 agtcgccgca tacgcggcct cgcgcgcctt cttccactcc tgcttgctcg caaacagggt
     4861 gcccgaggtc tggccgacca gctctcccgg gccggcgcct accatgtcct cgaggaaacg
     4921 gttgcagcgc tggatggcgc ggttgcgcac gaacgcgatg ccgaccgtgg cgttgtcgag
     4981 gatcagctcc tgctcggcga gcgcccgctg cacgcgctcc tcggccgcgt gctcctgcgt
     5041 gatgtcgtcg aacagccata ccgagccctc cgacggatca ccgggctgca ccgcgcgccc
     5101 ggagatcgag caccagatct gcgtgccgtc cttgcgcacg tgcgtccgct cgaggtactg
     5161 cgtctcgccc gcccacacca tctcgtagac ccggtcgccg ccggcggtga aatcctcgtc
     5221 gctcgcgaac atgaagcgcg tggtgcggtt cagcagctcg ccctcggcgt agccgaagat
     5281 ctcctcaaag cgccggttgc agcgcacgat cctgcgctcc ttgacgaaga tgatgccggc
     5341 gagcgcgttc tgcagcaccg cgtcctgctc gcgcaccacg cgctcgagca cttcgtcggc
     5401 gcgcttgcgc tcggagatgt cctcgaacag gaacacgtag cccttggacg gctcgcccgc
     5461 attgaccgcg cgcgccgaga tccggcacca gaagcccgag ccgtcctgct tgcgcaccca
     5521 gctttcgcgg gcgtgcgtgc gccctgcgtc gagctcgccg taggtctgcc cgcggcgctc
     5581 gaattcctcg tcggtgaagt acatctcgcg ccacgaggca ccgagcgcct cgccggtgcc
     5641 gaagccgaac atttcctcga agcgacggtt gcagcggatc accttgcggc tgcggc
//
LOCUS       DASPXW010000390         2499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_866932, whole genome shotgun sequence.
ACCESSION   DASPXW010000390 DASPXW010000000
VERSION     DASPXW010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866932"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VEL04_08985"
     CDS             <1..418
                     /locus_tag="VEL04_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYT48543.1"
                     /translation="AAKLGIVGLSKSIALDMAKYGVRSNCIAPFAWSRMIGSIPTETD
                     EQKARVEKLKSMQTAKIAPLAVFLASEKAKDVSGQIFAVRANELFVMSQSRPLRSVHR
                     AEGWTPQTIASHALPALRAHFYPLERTQDVFSWDPL"
     gene            424..1353
                     /locus_tag="VEL04_08990"
     CDS             424..1353
                     /locus_tag="VEL04_08990"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYT48544.1"
                     /translation="MIALLLVAGNAAAQSYPSRPIKIIVPLAAGGTGDTIARLLGEEL
                     GKILSGAVIIENRPGSGGVIGTEAVAKSAADGHTLLHTSSSHVLNAALRTKLPYDPLR
                     DFAPISRTADTYQVLLAHPSLPASTLNELIPLAKRNRILYGSAGNGSATHLNMELFKS
                     LAGIELEHVPYKGSTQARIDLLAGQIQLAIDGLLPTLPHIRAGRLKALAVTNSRRSAA
                     APEIPTIAETVPGYQSDTWYALLGPSAIPLPILKILEEKTRSALASPALREKFAQQGA
                     EPAAGGPQALDALMRAELARWSKVVVDAHVQVD"
     gene            1356..2324
                     /locus_tag="VEL04_08995"
     CDS             1356..2324
                     /locus_tag="VEL04_08995"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HYT48545.1"
                     /translation="MKRVLLTDSIAPAGEQALARAAEVVRAPDSRPETIRQFAREADG
                     IITRSKLPDDIFSAAPRVRAVAVHGTGTDLVPLADATARGVMVANLPGINAQSVAEYC
                     AMAMLMLSRNLLSIVGALKSEGWDNARALGSSAHEIAGMLLGIVGVGEIGSRLAKICA
                     SGFGMQVLGHQRRLDRLPPQVRPAALEELVKASDFIVLACPLTAETRHLFNQKMLSLM
                     KPGAWLINIGRGAVIHEEALIEALRAKRIAGAMLDVYEHYRLAPGHPLLALDNVILTP
                     HLAAVTQESRARAGIAAAEEMLRMLAGQRPQNFVNPGSWEKIYSKP"
BASE COUNT          451 a          874 c          770 g          404 t
ORIGIN      
        1 cgcggccaag ctaggcatcg tcgggctttc gaaatcgatc gcgctcgaca tggcgaaata
       61 cggcgtgcgc tccaactgca tcgcgccctt cgcctggagc cgcatgatcg gctcgatccc
      121 aaccgagacc gacgagcaga aagcgcgcgt cgagaagcta aagagcatgc agaccgccaa
      181 gattgctccg cttgccgttt tcttggcctc tgaaaaagcc aaagacgtga gcggccagat
      241 cttcgcggtg cgcgcgaacg agctcttcgt catgtcgcag agcaggccgc tgcgctcggt
      301 gcaccgcgcc gaaggctgga cgccgcagac catcgcctcg catgccctgc ccgcgctgcg
      361 cgcgcacttc tatccgctcg agcgcacgca ggacgtgttc tcctgggatc cgctgtgagg
      421 ctcttgatcg cccttctgtt agtggcggga aacgccgccg cgcaatccta tccctccagg
      481 cccataaaaa tcatcgtgcc tctcgccgcc ggcggcaccg gcgacaccat cgcgaggctg
      541 ctaggcgagg agctcggcaa gatcctcagc ggcgcggtga tcatcgagaa ccgccccggc
      601 tccggaggcg tgatcggcac cgaggcagtg gcgaagagcg cggccgacgg ccataccctg
      661 ctccacacca gctcctcgca tgtcctgaac gcggccctgc gcacgaagct cccttatgac
      721 ccgctgcgcg atttcgcgcc gatttcgcgc accgcggaca cctatcaggt gttgctggcg
      781 catccgtcgc tgcctgcgag cacactcaac gaactcattc cgctcgcgaa aagaaaccgc
      841 atcctctacg gctccgcggg caacggttcg gcgacccacc tcaacatgga gcttttcaag
      901 tcgctggcgg gaatcgagct cgagcacgtc ccatacaagg gcagcacgca ggcgcgcata
      961 gacctgctcg caggccagat ccagctcgcg atcgacggcc tgctgccgac gctgccgcat
     1021 atccgcgccg gcaggctgaa agcgctcgcc gtgaccaact cccggcgctc cgccgcggcg
     1081 cccgagattc cgaccatcgc tgaaaccgtg cctggctatc agtccgatac ctggtacgca
     1141 ttgcttggcc cctctgcaat tcctcttccc atattgaaaa tattggagga aaaaacccgc
     1201 tccgcgctcg cctcccccgc gctgcgggag aaattcgcgc agcagggcgc cgagcccgct
     1261 gcgggcggcc cgcaggcgct cgacgcactg atgcgcgcgg agctggcgcg ctggagcaaa
     1321 gtagtcgtcg atgctcacgt gcaagtcgat tgagcatgaa gcgcgtcctg cttaccgatt
     1381 cgattgcacc tgccggcgag caggcgctgg cacgggccgc cgaggtggtg cgcgcgcccg
     1441 acagccggcc ggagacgatc cggcagttcg cccgcgaggc ggacggcatc atcacgcgca
     1501 gcaagctgcc tgacgacatc ttttccgccg cgccgcgcgt gcgagcggtc gccgtccacg
     1561 gcaccggcac cgacctggtg ccgcttgccg acgccacggc acgcggggtg atggtcgcca
     1621 atctgcccgg catcaacgcc cagtcggtag ccgaatattg cgccatggcg atgctgatgc
     1681 tttcgcgcaa tctcctgtcg atcgtgggcg ccttgaagag cgaaggctgg gacaacgccc
     1741 gcgccctcgg ctcgagcgcg cacgagatcg ccggcatgtt gctcggcatc gtcggcgtgg
     1801 gtgaaatcgg cagccggctg gcgaagatct gcgcgagcgg cttcggcatg caggtgctcg
     1861 gccaccagcg ccggctcgac cgcctgccgc cgcaagtgcg cccggcagcg ctcgaggagc
     1921 tggtgaaagc ctcggacttc atcgtcctcg cctgtccgct cacggccgaa acgcgccatc
     1981 ttttcaacca gaaaatgctt tccctgatga aaccgggcgc gtggctgatc aacatcggcc
     2041 gcggcgcggt gatccacgaa gaagcgctga tcgaagcgtt gcgggcgaag cgcatcgcgg
     2101 gagccatgct cgacgtgtac gagcattacc gcctcgcccc cgggcatccg ctgctcgcgc
     2161 tcgacaacgt catcctcacg ccgcatctcg cggcggtcac ccaggagtcc cgcgcgcgcg
     2221 ccggcatcgc cgccgccgag gagatgctgc gcatgctggc cggccagcgc ccgcagaatt
     2281 tcgtcaaccc cgggtcctgg gaaaagatat attcgaagcc atgaagaagc cgatccgctc
     2341 cgagcttggc tggtccaccg tcgagcgcat agtggtacgc ggcaaggacc tgccggggga
     2401 aatcctcggg tatctcaact tcggcgacat ggcctacctc gagctcaccg gcagggttcc
     2461 caacccgaag gaatcaaaac tcttcaacgc catggtggt
//
LOCUS       DASPXW010000391         2630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1500713, whole genome shotgun sequence.
ACCESSION   DASPXW010000391 DASPXW010000000
VERSION     DASPXW010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500713"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..919)
                     /locus_tag="VEL04_09000"
     CDS             complement(89..919)
                     /locus_tag="VEL04_09000"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HYT48546.1"
                     /translation="MTEPSGSGVGGELARAREAHGLALADVAQQLKFAPRQLEALEQE
                     RFDLLPGGTFVRGMVRSYARLLKLDADPLVGRIADRFDAPDSNRLADRYRQPVPFSDS
                     TRRSTFVYLGLSLGILAAVGGVAYKWYHERPGQKQLAGPAPAASQRPVEAPRPRPLPP
                     QAVPAQQPSVMLEAKTMTQEKATVGEKTVALQPPRPAGVHRIVLRCEEEAWLEVTDAR
                     GRQLLSSLNPAGTERVVQGRPPFNLVIGNAMHVRISYNDRPVDLQPHTKVEVARFTLP
                     "
     gene            complement(912..1688)
                     /gene="pilW"
                     /locus_tag="VEL04_09005"
     CDS             complement(912..1688)
                     /gene="pilW"
                     /locus_tag="VEL04_09005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HYT48547.1"
                     /translation="MKRLACAFLVALSAGCASQNDGPSVESGTIVGEVGDPRNRARVH
                     TELAALYFDRGNMAIALEELRVATAADSSYAPAHSMFGLVYMELRENQRAEASFERAL
                     RLSPNDPDISHNFGWFLCNTGREPESIKHFLQAIRNPLYAMPWRSYSAAGVCSLRTNN
                     QRDAEEFFQRALKLEPDEPASLLKLGQIRYRQGNMAEARKLVARYNKLLTPNAESLWL
                     ALRVERKLGERVAEQSFATQLRRRYPASPEYQSLQRGQFD"
     gene            complement(1685..>2630)
                     /gene="rlmN"
                     /locus_tag="VEL04_09010"
     CDS             complement(1685..>2630)
                     /gene="rlmN"
                     /locus_tag="VEL04_09010"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HYT48548.1"
                     /translation="DLAKTLRDKLAAVARIAAPQIVGDVAAADGTRKWLLKVDGANAV
                     EAVFIPQAHRGTLCISSQAGCVLDCAFCSTGKQGFNRNLSTAEIIGQLWLANRLLQGA
                     RPISNVVMMGMGEPLLNLDNVIPALRLMLDDDAYGLSRRRVTVSTAGVIPEMDRLRDE
                     CPVALAVSLHAPNDALRDRLVPLNRKYPIAQLLAACNRYLEKAPRDFITFEYVMLDGV
                     NDGDSHAREAAAIAARVRCKYNLIPFNPFPRTEFTRSGEERIRRFAAVLQRAGVTVTT
                     RKTRGDDIAAACGQLAGDVADRTRRAARRIHALKEVRA"
BASE COUNT          391 a          881 c          932 g          426 t
ORIGIN      
        1 tcggtgttgg tcatcgactg gacgactatc ggcgcgccgc cgccgatcag cacatcgccc
       61 accgccacct ggtgcgaacg ccttctactc atggcagcgt gaagcgcgcg acttcgacct
      121 tggtgtgagg ctgcaggtcg accggccggt cgttatagga gatgcgcacg tgcatcgcgt
      181 tgccgatcac caggttgaac ggcggccttc cctgcaccac acgctcggtt ccggctggat
      241 tgagcgatga caggagctgg cgcccccggg cgtcagtcac ctcgagccag gcttcctcct
      301 cgcagcgcag cacgatgcga tgcacgcccg ccggccgcgg cggctgaagc gcgacggtct
      361 tttcgcctac tgtcgctttc tcctgagtca tggtcttcgc ttcgagcatc acgctcggct
      421 gctgcgccgg gacagcctgc ggcggcagcg gccgcgggcg cggcgcctcg accgggcgct
      481 gcgaagccgc gggggccggg ccagcgagct gcttctgccc gggccgctcg tggtaccact
      541 tgtaggcgac cccgcccact gcggcaagga tgcctagcga gagcccgaga taaacgaacg
      601 tggagcggcg tgtgctgtcg gagaacggca ccggctgccg gtagcggtcg gcgagacggt
      661 tcgagtcggg ggcgtcgaag cggtccgcga tgcgcccgac gagcgggtcg gcgtcaagct
      721 tgaggagccg cgcgtagctt cgcaccatgc cgcgcacgaa ggtgccgccc ggcagcaggt
      781 cgaaacgctc ctgctcgagc gcctcgagct gcctgggcgc gaacttcagc tgctgcgcaa
      841 cgtcggcgag cgcaagaccg tgggcttcgc gcgcgcgcgc gagctcgccg ccgaccccgc
      901 tgccggaggg ctcagtcaaa ctgtccccgc tgcagcgact ggtattcggg cgaggccgga
      961 tagcgcctgc gcagctgagt cgcgaagctt tgctcggcga cccgctcgcc cagcttgcgc
     1021 tcgacgcgca gcgcgagcca cagcgactcg gcgttcggcg tgaggagctt gttgtagcgc
     1081 gccacgagct tgcgcgcctc tgccatgttg ccctggcggt agcggatctg cccgagcttg
     1141 aggagcgatg ccggctcgtc gggctcgagc ttgagcgcgc gctggaagaa ctcctccgcg
     1201 tcgcgctggt tgttggtgcg caagctgcag acgccggccg cggagtacga gcgccacggc
     1261 atcgcgtaca gcggattgcg gatcgcctgc aggaagtgct tgatggattc cggctcgcgg
     1321 ccggtgttgc ataggaacca gccgaaattg tggctgatgt cggggtcgtt cggcgaaagc
     1381 cgcagcgcgc gctcgaagct cgcctcggca cgctggttct cgcgcagctc catgtacacc
     1441 agtccgaaca tgctgtgggc cggcgcatag ctcgaatccg cagcggtggc gacgcgcagc
     1501 tcctcaagcg cgatggccat gttgccgcgg tcgaagtaaa gcgcagcgag ctcggtgtgc
     1561 acgcgcgcgc ggttgcgcgg atctccgacc tcaccgacaa tggttccgct ctcgaccgaa
     1621 ggcccatcgt tctggctcgc gcagcccgcg gacagcgcga cgaggaacgc gcaggcgagc
     1681 cgtttcacgc gcgcacctcc ttcagcgcat ggatgcgccg cgccgcgcgc cgcgtgcggt
     1741 cggcgacgtc gccagcgagc tggccgcagg ccgcggcgat gtcgtcgccg cgcgtcttgc
     1801 gcgtggtcac cgtgacgcct gcacgctgca gcaccgcggc gaagcgccgg atgcgctctt
     1861 cgcccgagcg cgtgaactcg gtgcgcggga acgggttgaa cgggatcagg ttgtacttgc
     1921 agcgcacgcg agccgcgatc gccgcggcct cgcgcgcatg gctgtcgccg tcgttcacgc
     1981 cgtcgagcat cacgtactcg aaggtgatga agtcgcgcgg cgccttctcg agatagcggt
     2041 tgcaggcggc gagcagctgg gcgatggggt atttgcggtt gagcggcacc aggcggtcgc
     2101 gcagcgcgtc gttcggcgca tgcagcgaca cggcgagcgc taccgggcat tcgtcgcgca
     2161 ggcggtccat ttcggggatc acgcccgcgg tcgataccgt gacccggcga cgcgacaggc
     2221 cgtaggcatc gtcgtcgagc atcagccgca aggctgggat cacgttgtcg agattgagca
     2281 gcggctcgcc catgcccatc atcaccacgt tcgagatcgg cctggctcct tgcagaagac
     2341 ggttagcgag ccagagctgc ccgatgatct ccgcggtgct caggttgcgg ttgaagccct
     2401 gcttgccggt cgagcagaac gcgcagtcga gcacgcagcc cgcctggctc gagatgcaaa
     2461 gcgtgccgcg gtgagcctgc gggatgaaca cggcctcgac cgcattggcg ccgtcgacct
     2521 tgagcagcca tttgcgcgtg ccgtccgcgg cagccacgtc gcccacgatc tggggcgccg
     2581 cgatgcgggc aactgccgcg agcttgtcgc gcagcgtctt cgcgagatcg
//
LOCUS       DASPXW010000392         4574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1252582, whole genome shotgun sequence.
ACCESSION   DASPXW010000392 DASPXW010000000
VERSION     DASPXW010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4574
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252582"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VEL04_09015"
     CDS             <1..579
                     /locus_tag="VEL04_09015"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYT48549.1"
                     /translation="VPDLTVLAKILAGGLPGGAVAGARDILDQLDAGAAKAAGREKIG
                     HQGTFNANPLCAAAGVTMLSLVESGDACARAEATAEELRVGMRRILVEEGVPWGVFGE
                     ASVFVIFPNPARLPVDPANFDPARLGFRRLKGARDPQLNNRLRVALLAHGVDIAGGPG
                     GFVSEAHGAREVTQTLDAWRTALRALKADGLL"
     gene            663..1589
                     /locus_tag="VEL04_09020"
     CDS             663..1589
                     /locus_tag="VEL04_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYT48550.1"
                     /translation="MSTARWSLVAAIATMVAWGVNFAFVKYLLEHLGLGAFLFIRFAT
                     LPVLGCVLLVAIFRRRILHTWPRRADLPRFVACGLIGHTLHIAAVMYGMNLSTAFSSS
                     LVLTSGPLFTLMILAALGTERLRPRQVAGTLLACAGIVLFLGEKFSGGLARAGAGDLM
                     LLAAALLFSLYTVLIQPLAARYGPLIVLAYTLLFGAPPILVLCMQSFVDANLAGLTAA
                     VWVGLFWAIVISSFFGWLTWTWINTVRGVARSAPFAYLMPPIAGLVAWLTLGEVFTWL
                     KIAGAAVTMTGVAWAQFGGGSPPKATAQPDAA"
     gene            1586..2746
                     /locus_tag="VEL04_09025"
     CDS             1586..2746
                     /locus_tag="VEL04_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYT48551.1"
                     /translation="MKPIPRTVWALGLVSLFMDLSSELVHSLLPVFMTTVLGASMLAV
                     GMVEGVAEATASVVKLFSGWLSDRLGRRKPLVVFGYGLAALSKPLFPLAGSVALVLGA
                     RFIDRIGKGIRGAPRDALIADITASEGRGAAYGLRQALDSVGAVLGPLAAVGLMLLLA
                     SEVRAVLWLAVIPALVSVAILVVFVREGTEKIASKEKLSLRSAGGLGGKYWAIVALGA
                     VLTLARFSEAFLVLRGQDLGMALAFVPLVLVVMNLAYTAIAYPAGAAADRGHRAALLI
                     WGFVALIAADLLIATTASVTALFAGVVLWGAHMGLTQGLLSALVADAAPARLRGTGFG
                     VFHLVSGVVLLPASVLAGWLWSTHGAAATFYAGAIFTALALVGLLTILQKRS"
     gene            2750..3301
                     /locus_tag="VEL04_09030"
     CDS             2750..3301
                     /locus_tag="VEL04_09030"
                     /inference="COORDINATES: protein motif:HMM:NF025595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HYT48552.1"
                     /translation="MSGGHGHVEHHGGNKGVALLISILALVLAFAETLGKSAQTNALA
                     MNVEAANLWAFFQAKTIRQTVLRTAAEQTEILHATQNSKKQVEAWRKTAQRYQSEPET
                     GEGRDQLAARAKQAENKRDASMAAYHHYELASGVVQIAIVLASASIIAEIAALVWIAA
                     GLGALGVAFCLIGFFWPTAVHLF"
     gene            complement(3303..>4574)
                     /locus_tag="VEL04_09035"
     CDS             complement(3303..>4574)
                     /locus_tag="VEL04_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HYT48553.1"
                     /translation="WYAYDFFVIHRFGSLLTLGSVFLAITGAEALYADMGHFGKGAVR
                     VAWFSLVAPALVLNYFGQGALLMLQPAALQNPFYLLFPGWALYPMIALATAATVIASQ
                     ATISGAYSLTKQAVQLGFLPRMTIVHTSAREMGQVYIPSINWLLCAVVIAAVIGFGSS
                     SRLAGAYGVAVTATMLVDTLLTFFVIRYGWGYPLALCILATGFFALVDLAFFSSTLLK
                     VADGGWFPLVIGAGVYLTMATWRRGREMLFETLNSTSVPLAPFLESLFKDPPQRVPGT
                     AVFLTATPDATPHALLHNLNHNKVLHERVVFLTVQIRDVPWVPFEERVTCERLGHGCW
                     RVRVRYGFMNRPDVTSALELCGALGLQLETMETSFFLSRQKIVPTEGGAGMALWRDRV
                     FAAMARNAGNVTDYFNIPANRVIELGTRVQI"
BASE COUNT          676 a         1526 c         1592 g          780 t
ORIGIN      
        1 gttcccgatc tcaccgtgct cgccaagatc ctcgcgggcg gtcttccggg cggagcggtc
       61 gcgggcgcga gggacatcct cgaccagctc gacgccggcg cggcaaaggc cgccgggcgc
      121 gagaagatcg gccatcaggg caccttcaac gccaatccgc tctgtgcggc cgcgggcgtg
      181 accatgctgt cgctcgtcga gagcggcgac gcctgcgcgc gcgcggaagc gaccgcagag
      241 gagctgcgcg tcggaatgcg ccgcatcctg gtcgaggaag gcgtgccatg gggcgtgttc
      301 ggcgaggcct cggtgttcgt catctttccc aaccccgcac gcctgcctgt cgatccggcg
      361 aatttcgatc cggcgaggct cggcttcagg aggctgaagg gcgcgcgcga tccgcagctc
      421 aacaaccggc tgcgcgtcgc gcttctcgca cacggcgtcg acatcgcggg cggccccggc
      481 ggcttcgtgt cggaggcgca cggcgcgcgc gaagtcactc agacgctgga cgcttggcgg
      541 accgcgttgc gcgcgctgaa ggcggatggc cttctatgag gcgagcgcgc gtcccagcct
      601 tgcgacgccg tccgccggcg tttcgaatct catgaacgcg tctattctag actgcgcgca
      661 ccatgtcgac tgcgcgctgg tccctggtgg ccgccatcgc aaccatggtg gcctggggcg
      721 tgaacttcgc gttcgtgaag tacctgcttg agcacctcgg cctgggcgcg tttcttttca
      781 tccgtttcgc gaccctgccg gtgctcggct gcgtgcttct tgtagcgatc ttcagaagac
      841 gaatcctgca tacctggccg cgccgcgccg acctgccgcg cttcgtcgcc tgcggcctga
      901 tcggccatac gctgcacatc gccgcggtaa tgtacggcat gaacctgtcg accgcgttct
      961 cctcgtccct ggtgctgacg agcgggccgc tgttcacgct gatgatcctc gcggcgctcg
     1021 gcaccgagcg cctgcggccg cggcaggtcg cgggcacgct gctcgcctgc gccggcatcg
     1081 tgctgttcct cggcgagaag ttcagcggcg gcctcgcccg cgccggggcc ggcgacctga
     1141 tgctgctcgc cgccgcgctg ctgttttccc tgtacaccgt cctcatccag cccctcgcgg
     1201 cgcgctatgg cccgctgatc gtgctcgcat atacgctgct gttcggtgca ccgccgatcc
     1261 tcgtcctctg catgcagagc ttcgtcgatg caaacctcgc cggtttgacc gcggcagtct
     1321 gggtcggcct gttctgggcg atcgtgatct cgtctttctt cggctggctg acgtggacct
     1381 ggatcaacac ggtgcgcggg gtcgcgcgct cggcgccgtt cgcgtacctc atgccaccga
     1441 tcgccggcct ggtcgcctgg ctcacgctcg gcgaggtttt tacctggctc aagatcgccg
     1501 gcgcggcggt gacgatgacg ggggtcgcct gggcgcaatt cggcggcggc tcgccgccga
     1561 aggcgacggc gcagcccgac gcggcatgaa accgatcccg aggacggtct gggcgctcgg
     1621 actggtgtcg ctgttcatgg acctgtcctc ggagctggtg cacagcctgc tgccggtgtt
     1681 catgaccacg gtgctcggcg ccagcatgct ggcggtcggc atggtcgaag gtgtcgcgga
     1741 agcgaccgct tcggtggtca agctgttctc cggctggctc tccgaccgcc tcggccggag
     1801 aaaaccgctg gtggttttcg gctacggcct cgcggcgctc tccaagccgc tgtttccgct
     1861 cgccggctcg gtggcgctgg tgctcggcgc ccgcttcatc gaccgcatcg gcaagggaat
     1921 ccgcggcgcg ccgcgcgacg ccctgatcgc cgatatcacg gctagcgaag gccgcggcgc
     1981 ggcctatggg ctgcgccagg cactcgactc ggtcggcgcg gtgctcgggc cgctcgcggc
     2041 cgtgggcctg atgctgctcc tggcgagcga ggtgcgcgcg gtgctgtggc tcgccgtcat
     2101 cccggcgctc gtctcggtcg ccatcctggt cgtattcgtc cgcgagggaa ccgagaaaat
     2161 tgcatcgaaa gagaagcttt cactgcgatc cgccggcggg ctcggaggca aatactgggc
     2221 gatcgtcgcg ctcggcgcgg tgctgacgct ggcgcggttc tcggaagcct ttctcgtgct
     2281 gcgcgggcag gacctcggca tggcgctcgc tttcgtgccg ctggtgctgg tggtgatgaa
     2341 cctggcctac acggcgattg cctacccggc gggcgccgcc gccgaccgcg ggcatcgcgc
     2401 ggcgctcctg atctgggggt tcgtggccct gatcgccgcc gatcttctga ttgcaaccac
     2461 ggcttcggtg actgcgcttt ttgccggcgt ggtcctgtgg ggcgcgcaca tggggctgac
     2521 gcaggggctg ctgtcggcgc tcgtcgccga cgccgcgccc gcgcggctgc gcggcaccgg
     2581 cttcggcgtg ttccacctcg tctccggggt cgtgctgctc ccggcgagcg tgctcgcggg
     2641 ctggttgtgg agcacgcacg gcgcggcagc cacgttttat gcgggggcga ttttcacggc
     2701 gctggcgctt gtcggcttgc ttacaatatt gcaaaagagg agctagggca tgtcgggggg
     2761 acacgggcac gtagagcatc acggcggcaa caagggcgtc gcgctgctga tctcgatcct
     2821 ggcgctggtg ctggcgttcg ccgagacgct cggcaagagc gcgcaaacca atgctctcgc
     2881 gatgaacgtc gaggctgcca acctgtgggc gttcttccag gcgaaaacca tccgccagac
     2941 ggtattgcgc accgcggccg aacagaccga gattttgcat gccacccaga attcaaaaaa
     3001 acaagtcgaa gcgtggcgca agacggcgca gcgctaccag tccgagcccg agaccggcga
     3061 gggccgcgac cagctcgcgg cgcgcgccaa gcaagcggag aacaagcgcg acgcctccat
     3121 ggccgcctac caccactacg agctggcatc gggcgtggtg cagattgcca tcgtgctcgc
     3181 ctcggcgtcg atcatcgccg aaatcgcggc tctggtctgg atcgccgcgg ggctcggtgc
     3241 gctcggcgtc gcgttctgcc tgatcggctt tttctggccg accgcggtgc acctgttcta
     3301 ggtcagatct gcacccgtgt gccgagctcg atgacgcggt tcgcggggat gttgaagtag
     3361 tcggtgacat tgccggcatt gcgcgccatc gcggcgaaca cgcggtcgcg ccacagcgcc
     3421 atgccggcgc cgccttcggt aggcacgatc ttctgccgcg acaggaagaa ggaggtttcc
     3481 atcgtctcga gctgcaggcc gagcgcgccg cacagctcta gcgcgctggt gacgtcgggc
     3541 cggttcatga agccgtagcg cacgcgcacg cgccagcagc cgtggccgag ccgctcgcag
     3601 gtcacgcgct cctcgaacgg tacccagggc acgtcgcgga tctgcaccgt gaggaacacc
     3661 acgcgctcgt gcagcacctt gttgtggttg aggttgtgca gcagcgcgtg cggcgtcgcg
     3721 tccggcgtgg cggtgaggaa caccgccgtg cccggcacgc gctgcggcgg gtccttgaac
     3781 agggactcga ggaacggcgc cagcggcacc gaggtggaat tgagcgtctc gaacagcatc
     3841 tcgcgcccgc gccgccaggt tgccatggtg aggtacacgc cggcgccgat caccagcggg
     3901 aaccagccgc cgtcggcgac cttgagcagc gtcgaggaga agaacgccag gtcgaccagc
     3961 gcgaagaacc cggtcgccaa gatgcacagc gcgaggggat agccccagcc gtagcggatc
     4021 acgaagaaag tgagcagcgt gtcgaccagc atggtcgccg tgaccgcgac gccgtaggcc
     4081 ccggcgagcc gcgacgaaga gccgaagccg atcaccgccg cgatcaccac cgcgcacagc
     4141 agccagttga tcgacgggat gtagacctgg cccatctcgc gcgccgaagt gtggacgatg
     4201 gtcatgcgcg gcaggaagcc gagctgcacc gcctgcttgg tgagcgaata cgcgcccgag
     4261 atggtggcct gcgaggcgat cacggtggcg gcggtggcga gcgcgatcat cgggtacaac
     4321 gcccatccgg gaaacagcag atagaaggga ttctgcagcg ctgcgggctg gagcatgagc
     4381 aacgcgccct gcccgaagta gttgagtacc agcgccggcg ccaccaggct gaaccaggcg
     4441 acgcgcaccg cgcctttgcc gaagtggccc atgtcggcgt agagcgcttc cgcgccggtg
     4501 atcgccagga acacggagcc gagcgtcagc agcgatccga accggtggat gacgaagaag
     4561 tcgtacgcgt acca
//
LOCUS       DASPXW010000393         3468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1253121, whole genome shotgun sequence.
ACCESSION   DASPXW010000393 DASPXW010000000
VERSION     DASPXW010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253121"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VEL04_09040"
     CDS             complement(<1..482)
                     /locus_tag="VEL04_09040"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HYT48554.1"
                     /translation="MDRPRYDPALEREFIARGWWAGDTLPRWLARHAAERPDQPAIVS
                     SQDLITWKRLQERVLRAAQGLREKGVGRGDVVAVQLPNVPEFIIAHLAVNRLGAVLCT
                     LHMPYRGAEIEAILGHSGAKLFLDSPSLVTDLEENSVLENNYPLPDARDPFLLLYTSG
                     T"
     gene            503..1795
                     /locus_tag="VEL04_09045"
     CDS             503..1795
                     /locus_tag="VEL04_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HYT48555.1"
                     /translation="MAEALDEALLLARFQFGLNIGFHILFPSITIGLAWIVAYFRIRY
                     EATRDAAWLRTYKLWVRVFALTFAMGVVSGVTMSFQFGTNWPGYMNHVGNIAGPLLAY
                     EVLTAFFLEATFLGVMLFGMERVSGRVHVASSVIVAFGTTLSAFWILALNSWMHTPAG
                     HVDSASGIIAGSWLEVIFNPSFPYRLAHMLLASGITAALLVAGLSAWRLLAAPQDAAA
                     LKTLRFGARLAALLVPLQIVAGDLHGLNTREHQPAKIAAIEALWETEQGAPLVLFALI
                     DEKEKANRFALEVPKAASLVLTHKLDGEVKGLAEFGDDHPPVAPVFTAFRVMVGMGFL
                     MLALAWGSQPWLKDPPRWLLWTFAGFTFAGWIATLAGWLVTEIGRQPWLVTGILRTAD
                     AAGEASGAELGASLTAYVLTYGALLLAYMVTLTHMARK"
     gene            1798..2766
                     /locus_tag="VEL04_09050"
     CDS             1798..2766
                     /locus_tag="VEL04_09050"
                     /EC_number="7.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006119 - oxidative phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HYT48556.1"
                     /translation="MDLPLVFAALMGVAMLAYVVLDGYDLGVGMLMPAAGREEQDLMV
                     ASIGPFWDANETWLVLGIGLLLVAFPIAHGEILGALYLPVAAMLVGLMLRGVAFELRI
                     KAEGWQRELWNWLFWAGSTLASFAQGLMLGRYITGFEPGVGYWLFAIAVGASLCGGYV
                     LLGATWLVLRTEGHLRERAVGWAKWGLMWVALGVALVSLGTPLTSETVRDKWFDFPRT
                     LYLMILPALTIAAGFWTFSSLKKSDWQPFAGAVAIFVLAFAGLAYSLFPYVVMDRLTI
                     WQAAAHPSALVFMLVGALIVLPFIVGYTIYSYRVFRGKVRGGEPYG"
     gene            complement(2771..>3468)
                     /locus_tag="VEL04_09055"
     CDS             complement(2771..>3468)
                     /locus_tag="VEL04_09055"
                     /inference="COORDINATES: protein motif:HMM:NF024584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HYT48557.1"
                     /translation="HEAIRVGSDFAKLLENLRRFLSLRRDAGSAAPKVEIFYLMTPGN
                     LAELPQAVELAAELGADELVATNLDYVISPGLDALRAYSASPPAQARRAALDAAAAAA
                     RAAGIGFRPYRLQFREMAVCDLNPLRILFVASDGSVSPCVYTSLAGPTEMPRVFEGRP
                     LETPLVSFGNVNQRPLMEIWESAAYREFRGRLARRVAGAGLAMLGAAAGAAADEELPA
                     PEACRTCPKLYGV"
BASE COUNT          507 a         1161 c         1190 g          610 t
ORIGIN      
        1 gttcccgagg tgtagagcag caggaacggg tcgcgcgcat cgggcagggg atagttgttt
       61 tcaagaaccg aattttcctc caggtcagta actagtgaag gcgaatccag aaacagcttc
      121 gcgccgctat gccccaggat cgcctcgatc tcggcgccgc ggtaaggcat gtggagggtg
      181 cagagcacag cgccgagccg gttcacggcg aggtgggcga tgatgaactc cggcacgttc
      241 ggcagctgca ccgccaccac gtcgccgcgc ccgacgccct tttccctgag cccctgggca
      301 gctctcagta cccgctcctg gagcctcttc cacgttatga gatcttgtga agaaacaatg
      361 gcaggctgat cgggccgctc cgcggcatgg cgtgcaagcc agcgcggcag cgtgtccccg
      421 gcccaccagc cgcgcgcgat gaattcgcgc tcgagcgccg ggtcgtaccg cggcctgtcc
      481 atcccccgta tattagcgtc gcatggcaga agccctggac gaagccctgc tgctggcccg
      541 cttccagttc gggctgaaca tcggcttcca tatcctgttc cccagcatca ccatcgggct
      601 cgcctggatc gtcgcctact tccgcatccg ctacgaggcc acgcgcgacg cggcatggct
      661 gcgcacctac aagctctggg tgagggtgtt cgcgctgacc ttcgccatgg gcgtggtgtc
      721 gggcgtgacc atgagcttcc agttcggcac caactggccg ggctacatga accacgtcgg
      781 caacatcgcc gggccgctgc tcgcctacga ggtgctcacc gcattcttcc tcgaggcgac
      841 gttcctcggc gtgatgctgt tcggcatgga gcgggtctcg gggcgcgtgc acgtcgcatc
      901 gagcgtcatc gtcgccttcg gcaccacgct gtcggcgttc tggatcctcg cgctcaactc
      961 ctggatgcac acgccggccg gccatgtcga ttccgcttcc ggcatcatcg ccggcagctg
     1021 gctcgaggtg atcttcaatc cgtcgtttcc ctatcgcctc gcccacatgc tgctcgcctc
     1081 gggaatcacg gcagcgctgc tggttgcggg actctcggcg tggcggctgc tcgcggcgcc
     1141 gcaggacgcg gccgcgctca agaccctgcg cttcggcgcg cggctcgcgg cgctgctggt
     1201 gccgctgcag atcgtcgccg gcgacctgca cgggctcaat acgcgcgaac accagccggc
     1261 gaagatcgcc gccatcgagg cgctgtggga gaccgagcag ggcgcgccgc tggtgctgtt
     1321 cgcgctgatc gatgaaaaag agaaggcgaa ccgcttcgcg ctcgaggtgc ccaaggcggc
     1381 gtcattggtc ctgacgcaca agctcgacgg cgaagtgaaa gggctcgccg agttcggcga
     1441 cgaccacccg ccggtcgcgc cggtgttcac cgcgttccgg gtgatggtgg gcatgggctt
     1501 tctcatgctc gcattggcgt ggggcagcca gccatggctg aaagatccgc cgcgctggct
     1561 gctctggaca ttcgccggct tcaccttcgc cggctggatt gccacgctcg ccggctggct
     1621 ggtgaccgag atcgggcgcc agccctggct ggtcaccggg atcctgcgca ccgcggatgc
     1681 ggccggcgaa gcgtcggggg cggagctggg cgcgagcctc acggcctatg tgctgaccta
     1741 tggcgctcta ttgcttgctt acatggtcac gctcacgcac atggcaagga aataggcatg
     1801 gacctgccgc tcgtcttcgc cgcgctgatg ggcgtggcga tgctcgccta cgtggtgctc
     1861 gacggctacg acctcggcgt cggcatgctg atgccggcgg ccgggcgcga ggagcaggac
     1921 ctgatggtgg cctcgatcgg cccattctgg gacgcgaacg agacctggct ggtgctcggc
     1981 atcggcctgc tgctggtcgc gttcccgatc gcgcacggcg agatcctcgg cgcgctctat
     2041 ctgccggtgg cggcgatgct ggtcgggctc atgctgcgcg gcgtggcctt cgagctgcgc
     2101 atcaaggccg agggctggca gcgcgagctg tggaactggc tgttctgggc cggctcgaca
     2161 ctggcgagct tcgcgcaggg gctgatgctc gggcgctaca tcaccggctt cgagccgggc
     2221 gtcggctact ggctgttcgc gatcgcggtc ggcgcgagcc tgtgcggcgg ctacgtgctg
     2281 ctcggcgcga cctggctggt gctgcgcacc gagggccatc tcagggaaag ggccgtcggc
     2341 tgggcgaagt gggggctgat gtgggtcgcg ctcggcgttg cgctcgtgag cctgggcacg
     2401 ccgctcacga gcgagacggt gcgcgacaaa tggttcgact tcccgcgcac gctttacctg
     2461 atgattctcc cggccctgac catcgccgcc ggtttctgga cattttccag tttaaaaaaa
     2521 tccgactggc aaccctttgc cggcgcggtg gcgatcttcg tgctcgcctt cgccggcctc
     2581 gcctacagcc tgttccccta cgtggtgatg gatcgcctga cgatctggca ggccgccgcg
     2641 cacccgagcg cgctcgtgtt catgctcgtc ggcgcgctga tcgtgctgcc gttcatcgtc
     2701 ggctacacga tatattccta ccgcgtgttc cgcggcaagg tcagaggggg agaaccctat
     2761 ggataaccca tcaaacgccg tagagcttgg gacaggtgcg gcaggcttcg ggcgcgggca
     2821 gctcctcgtc ggctgccgcg cccgcggcgg cgccgagcat cgccagcccg gcaccggcca
     2881 cgcgcctcgc cagccggccg cgaaactcgc ggtatgcggc gctctcccag atctccatca
     2941 gcgggcgctg gttgacgttg ccgaagctca ccagcggggt ctcgaggggc cggccctcga
     3001 acacgcgcgg catctcggtc ggaccggcga gcgaggtgta gacgcacggc gataccgagc
     3061 cgtcggaggc gacgaacagg attctcaggg gattgaggtc gcacaccgcc atttcccgga
     3121 actgcagccg gtaggggcgg aacccgatgc cggcagcgcg cgccgcggcg gccgccgcgt
     3181 cgagcgcggc gcggcgggcc tgcgccggcg gcgaggccga gtaggcccgc agcgcgtcga
     3241 gcccgggact gatcacatag tcgaggttgg tcgccaccag ctcgtcggcg ccgagctccg
     3301 ccgcgagctc gaccgcctgc ggcagctcgg ccaggttccc cggcgtcatg agatagaaga
     3361 tctcgacttt cggcgcggcg ctccccgcat cgcgccgtaa cgagagaaag cggcgcaggt
     3421 tttccagcag cttcgcaaag tcggagccga cgcggatcgc ctcgtggg
//
LOCUS       DASPXW010000394         2341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_239134, whole genome shotgun sequence.
ACCESSION   DASPXW010000394 DASPXW010000000
VERSION     DASPXW010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239134"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VEL04_09060"
     CDS             <1..245
                     /locus_tag="VEL04_09060"
                     /inference="COORDINATES: protein motif:HMM:NF015181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HYT48558.1"
                     /translation="DEQLKRLSPCDLLLVEGFKFAPIPKLEVWRAETGEALLHPNDPH
                     IVAIASDAPVETKLPFLNLNDHAAIEAFIVKHLKLA"
     gene            242..1444
                     /gene="glp"
                     /locus_tag="VEL04_09065"
     CDS             242..1444
                     /gene="glp"
                     /locus_tag="VEL04_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HYT48559.1"
                     /translation="MNQGLLSVDEALEQLLSGARAVSEVEEVPTLEATKRVLARPQRS
                     TMNVPPMDNSAMDGYAVRVSDLQSSMKLKVSQRIMAGMVGKPLEPGTAARIFTGAPIP
                     PGADAIVMQEHCESEKDAVVIKRVPKPGAWIRLTASDIQKGAEILAAGKRLLPQDTGL
                     AASVGIKTLPVFRKLRLGLFFTGDELVMPGEPLAPGRIYNSNRFTLRGLAEIFGCEVR
                     DYGIVPDSLAATRETLRRAAGECDVIVTSGGVSVGEADYVKPAVEAEGSLLMWRIAMK
                     PGRPLAFGSIQNAFFIGLPGNPVSSFVTFLIFVRPFLLKTQGVTEVAPRAIAARADFD
                     WPTPDARREFLRVKWNAQGGLELYPTQDSAVLTSTAWADGLVDNPAGHAIGRGEQVRF
                     LPYAELYW"
     gene            1441..1698
                     /gene="moaD"
                     /locus_tag="VEL04_09070"
     CDS             1441..1698
                     /gene="moaD"
                     /locus_tag="VEL04_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010115001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HYT48560.1"
                     /translation="MKVKLLFFAGLREQLGTSGEEIELPHGVTTAAGLKLHLLQRGGA
                     WKSAFSGGRLVRMAVNQDMVQPSARIKAGDEVAFFPPVTGG"
     gene            1700..2176
                     /locus_tag="VEL04_09075"
     CDS             1700..2176
                     /locus_tag="VEL04_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HYT48561.1"
                     /translation="MKISVQREDFDSGLEIRSLSKDRGIGAVASFIGVVREVPMTLEH
                     YPGMTENAIRKIAEEAVARWKIVDCTVIHRYGDLKPGDQIVLVAVAAAHRGEAFAACE
                     FIMDYLKTRAPFWKKEHRPEGAAWVEARVTDDAAAERWTEAPKPAALGKKRRSRSA"
BASE COUNT          409 a          736 c          797 g          399 t
ORIGIN      
        1 tcgacgagca attgaagcgc ctgtcgccct gcgatctgct gctcgtcgaa ggcttcaagt
       61 tcgcgccgat cccgaagctc gaggtgtggc gcgccgagac cggcgaggcg ctgctgcatc
      121 cgaacgatcc gcacatcgtc gccatcgcga gcgatgcgcc ggtggagacg aaactgcctt
      181 tcctgaacct gaacgatcat gcagcgatcg aagctttcat cgtgaagcac cttaagctgg
      241 catgaaccag gggctgcttt cagtcgacga ggcgctcgag caactgctct ccggcgcgcg
      301 cgccgtctcc gaggtcgagg aagtgccgac cctcgaggcg acgaaaaggg tgctcgcacg
      361 gccgcaacgc tcgacgatga atgtgccgcc gatggacaac agcgccatgg atggttatgc
      421 agttcgcgtt tccgatcttc aatcttcaat gaaactgaaa gtctcgcagc gcatcatggc
      481 cggcatggtg ggcaagccgc tcgagccggg gacggcggcg cgcatcttca ccggggcgcc
      541 gattccgccc ggggccgatg ccatcgtcat gcaggagcac tgcgaatcag aaaaggacgc
      601 ggtggtaatc aaaagagtgc cgaaacccgg ggcctggatc cgccttacgg ccagcgacat
      661 ccaaaagggc gccgagatcc tcgccgccgg gaagcgcctc ctgccgcagg acacgggcct
      721 cgcggcgtcg gtcggcatca agaccctgcc ggtgtttcgc aagctcaggc tggggctttt
      781 cttcaccggc gatgagctcg tgatgccggg cgagccgctc gcgcccgggc gcatctacaa
      841 ctcgaaccgc ttcacgctgc gcggcctggc ggagatcttc ggctgcgagg tgcgcgacta
      901 cggcatcgtc cctgacagcc tcgctgcgac gcgcgagacg ctgcgccgcg cggcgggcga
      961 gtgcgatgtg atcgtcacct cgggcggcgt ctcggtcggc gaggcggact acgtcaagcc
     1021 cgccgtcgag gccgaaggct cgctcctcat gtggaggatc gccatgaagc ccgggcggcc
     1081 cctggctttc ggcagcattc aaaatgcctt ttttatcggc ttgccgggca acccggtgtc
     1141 gagcttcgtc accttcctga ttttcgtgcg gccgttcctg ctgaaaacgc aaggtgtcac
     1201 cgaagttgcg ccgcgcgcga tcgcggcgcg cgcggatttc gactggccca cgcccgacgc
     1261 gcggcgcgaa ttcctgcgcg taaaatggaa cgcgcagggc ggcctcgagc tctatccgac
     1321 gcaggactcg gcggtgctga cctccaccgc ctgggccgac ggcctggtgg acaaccccgc
     1381 cgggcatgcg atcggcaggg gcgagcaggt gcgcttcctg ccctatgcgg agctgtactg
     1441 gtgaaagtga agctcttgtt cttcgccggc ctgcgcgagc agctcggcac ttcgggcgag
     1501 gagatcgagc tgccccacgg cgtgacgacc gcggcggggt tgaaattgca tctactgcag
     1561 cgagggggcg cctggaagtc ggcgttttcc gggggcaggc tggtccgcat ggcggtgaac
     1621 caggacatgg tccagccctc ggcgcgcatc aaggcgggcg acgaggtggc gttctttccc
     1681 ccggtgacgg gaggctgaga tgaagatctc ggtgcagcgc gaggattttg attctggttt
     1741 ggaaatacga agtctttcga aagatcgagg catcggcgcg gtggcgagct tcatcggcgt
     1801 ggtgcgcgag gtgccgatga ccctcgagca ctaccccggc atgaccgaga acgccatccg
     1861 caagatcgcg gaagaagcgg tggcgcgatg gaagatcgtc gattgcacgg tgatccaccg
     1921 ctacggcgac ctcaagcccg gcgaccagat cgtgctggtc gccgttgcag ccgcacaccg
     1981 cggcgaggcc ttcgccgcct gcgagttcat catggactac ctgaaaacgc gcgcgccgtt
     2041 ctggaagaaa gagcatcgtc ccgagggcgc ggcctgggtc gaggcgcgcg tcaccgacga
     2101 cgccgccgcg gaacgctgga ccgaagcacc caagccggct gcgctgggca agaagcgtcg
     2161 ctcgcgaagc gcctagggct gcgggccttc gtatccctcg atgatgacga gatcgatggt
     2221 ggagacgtcc ttgcgcagct tgattgcccg ctggtaatcg ggcgccttcc agcagtcgag
     2281 ggccgcctga taggtcggaa attcgatcac tgcgttgcgg gtgcggctcg cgccctcggg
     2341 a
//
LOCUS       DASPXW010000395         2083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_490767, whole genome shotgun sequence.
ACCESSION   DASPXW010000395 DASPXW010000000
VERSION     DASPXW010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490767"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="VEL04_09080"
     CDS             complement(<1..634)
                     /locus_tag="VEL04_09080"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYT48562.1"
                     /translation="MSEAALKKTSADPRVAAAIAHWAPRFVSNGVLLADFEEVTAGIG
                     RWEDWCRAWCERAKVHETLGREALKEGYKLSAGEHLVRAAMYYHFAKFVFVHDQKQMR
                     DAHLRSVDCYKAALPLMRPTGERAAIPFEGKTLFGILRHPLQPSVRSPVLIMAPGLDS
                     TKEELHAYEEPFLARGIATLAIDGPGQGEAEYEIPICGDYERAAKAVCDWI"
     gene            complement(787..1041)
                     /locus_tag="VEL04_09085"
     CDS             complement(787..1041)
                     /locus_tag="VEL04_09085"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HYT48563.1"
                     /translation="MKMLSVRLDDKEAAALDAVCEAQGLSRSEVVKRAIRDLAQAGRR
                     GSFGRVARDLGLIGCFSGPSDLGEHHGKYFRRMLGAKTAR"
     gene            complement(1146..1754)
                     /locus_tag="VEL04_09090"
     CDS             complement(1146..1754)
                     /locus_tag="VEL04_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48564.1"
                     /translation="MTSTAQAQQGSMTFFVTSAGKGNGADLGGLEGADAHCAALAKAA
                     GAKATNWRAYLSTWLPGGDAGVNARDRIGKGPWQNAKGVVVAKSVNDLHSERNNINKQ
                     TALTEKGEVLSGRGDPVNVHDILTGSDPDGRYSTAGGDTTCGNWTKSGEGSAIVGHHD
                     LQGLKDTRHMKSWNSSHGSRGCSQESLKASGGAGLVYCFVAN"
     gene            complement(1870..>2083)
                     /locus_tag="VEL04_09095"
     CDS             complement(1870..>2083)
                     /locus_tag="VEL04_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008645348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48565.1"
                     /translation="CTYPLTGIGVVKRVYTDLAVIEVTPRGLVARELVPGLAIEELQR
                     LTGMPLLPSDAIAAAADLSRGKCVGL"
BASE COUNT          348 a          705 c          651 g          379 t
ORIGIN      
        1 cgatccagtc gcagaccgcc ttggccgcgc gctcgtagtc gccgcaaatc gggatctcgt
       61 actccgcctc gccctgcccc ggcccgtcga tggcgagcgt ggcgatgccg cgcgcgagga
      121 acggctcctc gtaggcgtgc agctcctcct tggtggaatc caggcccggc gccatgatga
      181 gcacgggcga tcggacggaa ggttgaagcg ggtgccgcag gattccgaaa agggtcttgc
      241 cctcgaacgg tatcgcggcg cgctcgcctg tcgggcgcat gagcggaagc gccgccttgt
      301 agcaatcgac cgagcgcagg tgcgcgtcgc gcatctgctt ctgatcgtgc acgaaaacga
      361 acttggcgaa gtggtagtac atcgccgcgc gcaccaggtg ctcgccggcg ctcaacttgt
      421 agccttcctt cagcgcctcg cgcccgagcg tctcgtgcac cttggcgcgc tcgcaccagg
      481 cgcggcacca gtcctcccag cgcccgattc cggctgtgac ctcctcgaaa tcggcgagca
      541 gcactccgtt cgacacgaag cgcggcgccc agtgcgcgat cgccgccgcg acgcgcgggt
      601 cggcggaggt tttcttcagg gccgcttcgg acatgcggat tctcgaactg tcatttggga
      661 aaacgccgcg accggcgggg tgactgcgac cgtcgtgatc agggctccat caaaacgttc
      721 gaaaaacgcg acggcggtcg agtgctgccg ttcggccgtt cgccagcgcg accagcgggg
      781 ccggtgtcaa cgagcagtct tcgcaccgag catgcggcgg aagtatttgc cgtggtgttc
      841 gcccagatcg cttgggcccg agaaacaccc gatgaggccc aggtcgcgcg ccacccgccc
      901 gaacgatcca cggcgcccgg cctgggcgag atccctaatg gcgcgcttga cgacctccga
      961 gcgcgacagg ccctgcgcct cgcataccgc atcgagcgcg gcggcctcct tgtcgtcgag
     1021 gcgcaccgag agcattttca tatatcgcac tcgagaaaac tgtatgacaa gcatcataca
     1081 ctcgcctagt ccgagccaag aggggttgtg caggcccgac tcgggtcggt ccgtccggcc
     1141 ttgcgtcagt tcgcgacgaa gcaatagacc aggccggcgc cgccggaggc tttcaggctt
     1201 tcctgactgc agccgcgcga gccatgcgag gagttccagg acttcatatg ccgggtgtcc
     1261 ttcaatcctt gcaggtcatg gtggccgacg atcgccgagc cttcgccgct ctttgtccag
     1321 ttgccgcacg tggtgtcgcc gcctgccgtc gagtaacgcc cgtccgggtc cgagcccgtc
     1381 aggatatcgt gcacgttcac cggatccccg cggccgctga gcacctcgcc cttctcggtc
     1441 agagctgttt gtttattgat gttgttgcgt tcggaatgca ggtcattcac gctcttggcc
     1501 accaccacgc ccttggcgtt ctgccagggg cctttgccga tgcggtcgcg cgcgttgacg
     1561 ccggcgtcgc cgcccggcaa ccacgtgctc aagtaagcgc gccagttcgt cgccttcgcg
     1621 cctgccgcct tggcgagcgc cgcgcaatgc gcatcggcgc cctccaggcc gccgagatcg
     1681 gcaccgttgc ccttgcccgc actggtcacg aagaacgtca tgcttccctg ctgagcctgc
     1741 gcggtcgatg tcatgcctgt tcccgctgcg accagcgaga ggaaaatact tgctgccgag
     1801 attctcgaaa aattcatctg acctccttgg gtgtattgag ttgaactaag gcggaaaacg
     1861 ccggccagat tataggccaa cgcattttcc cctgctaaga tcggccgccg cagctattgc
     1921 gtcagacggc agaagtggca tcccggtcag ccgctgcagc tcctcgatag cgagaccggg
     1981 caccagttca cgcgccacca gcccgcgcgg ggtgacctca atcaccgcga gatcagtgta
     2041 cacgcgcttc acgacgccga tgccggtgag cggataagtg cag
//
LOCUS       DASPXW010000396         4164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1752916, whole genome shotgun sequence.
ACCESSION   DASPXW010000396 DASPXW010000000
VERSION     DASPXW010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4164
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1752916"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VEL04_09100"
     CDS             complement(<1..654)
                     /locus_tag="VEL04_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48566.1"
                     /translation="MQYPASELVIDPVLIRSHNVAGPRYTSYPTADRFVEAFGETELR
                     QWLPKRNIGGISQPLSVYVHLPFCENVCYYCGCNKVVTRDHGRSAKYIKYLEKELALL
                     GPLLGADRKRQDLQLRFRRGQVGLCDAPLRRKQLRQVRVAVERDAVGTERDHLVERGI
                     VFVEALSVLIVGRPDPADRRDADPQHVAVGVSGIALEVALQAALRLGDRQLVAGTREV
                     "
     gene            688..1977
                     /locus_tag="VEL04_09105"
     CDS             688..1977
                     /locus_tag="VEL04_09105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HYT48567.1"
                     /translation="MISVKQSFLSHPVTIAVGVACALALLGLNVAAFTLGDRSLWLLG
                     GLLLGDMLIVGGCILIAARQVLVAEERTEASQARIEAIVDSAMDAIITVDAAQNIVLF
                     NRAAEQVFRCRRDQAIGAPLDRFLPQRWRQAHRGHIEQFGKTGVTSRRMGDVTTLWAL
                     RSDGEEFPIEASISQAGEPGQRYYTVILRDITVRKQYEDALKRQQQELRELSARVLEA
                     REEEKTRIARELHDELGQLLTALKMDLTWLRERLPDSELAKKADEMGEMLDRTVASTR
                     RISADLRPLMLDDLGLADAASWLVDDFGKRSGVRCEFQLAGNGALENASKAVATAVYR
                     ALQESLTNIARHSGAKHAWVMLAADDGEIQFEVEDDGRGIAPEDLAKVRSLGLKGMRE
                     RVTYLGGWFEVARAPRGGTRVRARMPFQKTMQEKAGG"
     gene            1974..2588
                     /locus_tag="VEL04_09110"
     CDS             1974..2588
                     /locus_tag="VEL04_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYT48568.1"
                     /translation="MIRVLIADDHEIVRDGLKRILAASADLQVAGEASSGDAALALVK
                     ANDYDVAMLDMSMPGLSGIALIKRLKLEKPKLRMLVLSMHGEHQYAARALKAGASGYL
                     TKDSAAEQLVGAIRKIAAGGVHISEAAAAALVASNSSHESLSDREFEVLRLLAEGFGP
                     TQIAERLHLSVKTVSTHKTRILQKLNLGSTAELVRYALEHKLLQ"
     gene            2649..3077
                     /locus_tag="VEL04_09115"
     CDS             2649..3077
                     /locus_tag="VEL04_09115"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYT48569.1"
                     /translation="MLRRVAARHTAGVQIYVVEDSRAVRERLIEMFSLVPHVRVVGHA
                     ATAEAAIHDILESKPDLVVLDLHLAEGSGFDVLRAVHPRAPGIEFYMLSNFSADPYRQ
                     LAERLGALGFFDKSKEFERVRDVVAKRARCHAKRADQTLS"
     gene            3139..3861
                     /gene="fnr"
                     /locus_tag="VEL04_09120"
     CDS             3139..3861
                     /gene="fnr"
                     /locus_tag="VEL04_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HYT48570.1"
                     /translation="MSQPEAPRKFEVTCSSCNLREMCLPGGVCLEDLERVENIVYARR
                     RIKRGETLFNAGDEFKSVYAIRSGFFKTSLLDGEGREQVTGFFMGGELLGMEGIGSGR
                     CTGTAIALEDSEVCVMPFALIEEMAREVPALQRHLHAVMAREIVRDHGVMMLLGSMRA
                     EQRLAAFLTNLSKRFVRRGYSASDFHLRMTRDEIGSYLGLKLETVSRLFSQFQKDGVI
                     EVEQKHVRILDSAGLEKILATR"
     gene            3869..>4164
                     /locus_tag="VEL04_09125"
     CDS             3869..>4164
                     /locus_tag="VEL04_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HYT48571.1"
                     /translation="MKTSFFAWSTAALLLSACGQRNDPYDVAKMDLKPVPAKISAERH
                     QGAFAEGAALSLGAELKPLDPSPVKTVRLDTTHRIIEIAPGVNFSAWTFGNQVPG"
BASE COUNT          739 a         1400 c         1371 g          654 t
ORIGIN      
        1 cacttcgcgc gtcccggcga cgagctgacg atcgcccagg cgcagggccg cctgcagcgc
       61 aacttccagg gctattccac tcacgccgac agcgacatgc tggggatcgg catctcggcg
      121 atcggccgga tcgggccgac ctactatcag aacagaaaga gcctcgacga atactatgcc
      181 gcgctcgacc aggtgatcgc gctcggtccc gaccgcatcg cgctctacag ctacgcgcac
      241 ctgccgaagc tgtttccgcc gcagcggcgc atcgcagagg ccgacctgcc ctcgccggaa
      301 gcgaagctgc agatcctgac gcttgcgatc ggcacccagc agcggcccca gcagcgccaa
      361 ttccttctcc aagtacttga tatacttcgc ggatcttcca tgatcgcggg tgacgacctt
      421 gttgcagccg cagtagtagc aaacgttttc gcagaacggc aggtgcacat agaccgagag
      481 cggctggctg atgccgccga tgttgcgctt cggcagccat tgcctcagct cggtctcgcc
      541 gaaagcctcg acgaagcgat ccgcggtcgg atacgaggtg tagcgcggtc cggctacgtt
      601 gtggctgcgg atcagcaccg ggtcgatgac gagctcggaa gcgggatatt gcattggggc
      661 actatgactg cggatgggcg ggacactttg atttcggtca aacagagctt tcttagtcat
      721 ccggtaacga tcgccgtcgg cgtcgcctgc gcgctcgcgc ttctcggcct caacgtcgcc
      781 gccttcacgc tcggcgaccg ctcgctctgg ctgctcggcg gcttgctgct cggcgacatg
      841 ctgatcgtcg gcggctgcat cctcatcgcc gcgcggcagg tgctggtggc cgaagagcgc
      901 accgaggcga gccaggcgcg gatcgaggcg atcgtcgatt cggcgatgga cgccatcatc
      961 accgtcgacg cggcgcagaa catcgtgctg ttcaaccgcg ccgccgaaca ggtgttccgc
     1021 tgccggcgcg accaggcgat cggcgcgccg ctcgatcgct ttctgccgca gcgctggcgc
     1081 caggcgcacc gcggccacat cgagcagttc ggcaagaccg gcgtcaccag ccggcgcatg
     1141 ggcgacgtga cgacgctctg ggcattgcgc tcggacggcg aggaattccc gatcgaggcc
     1201 tcgatctccc aggccgggga gcccgggcag cgctattaca cggtgatcct gcgcgacatc
     1261 acggtgcgca agcagtacga ggacgccctc aagcgccagc agcaggagtt gcgcgagctc
     1321 tcggcgcgcg tgctcgaggc gcgcgaggag gagaagacgc gcatcgcgcg cgagctgcac
     1381 gacgagctcg ggcagctcct caccgccctc aagatggatc tcacctggct gcgcgagcgg
     1441 ctgccggaca gcgagctggc aaagaaggcg gacgaaatgg gcgaaatgct cgacaggacc
     1501 gtcgcgtcca cgcgccgcat ctccgccgac ctgcggccgc tgatgctcga cgacctgggg
     1561 ctcgcagacg ccgcgagctg gctggtggac gatttcggca agcgctcggg agtgcgctgc
     1621 gagttccagc tcgccggcaa cggcgcgctc gagaacgcgt cgaaggcggt cgcgaccgct
     1681 gtttaccgcg cgctgcagga atcgctcacc aacatcgcgc ggcattccgg cgcgaaacac
     1741 gcctgggtga tgctcgccgc cgacgacggc gagatccaat tcgaggtcga ggacgacggc
     1801 cgcggcatcg cgcccgagga cctggcgaag gtacgctcgc tcggcctgaa aggcatgcgc
     1861 gagcgcgtga cctacctcgg cgggtggttc gaggtcgcgc gggcaccgcg cggcggcacg
     1921 cgcgttcgcg ccaggatgcc ttttcagaag acgatgcagg aaaaggccgg cggatgatcc
     1981 gcgtcctcat cgccgacgac cacgagatag tgcgcgacgg cctgaagcgc atcctcgccg
     2041 cctctgcaga cctgcaggtc gccggcgagg catcgagcgg cgacgcggcc ctcgccctgg
     2101 tgaaagccaa cgattacgac gtcgccatgc tcgacatgtc gatgcccggg ctttccggca
     2161 tcgcgttgat caagcggctg aagctcgaaa agccgaagct gcgcatgctg gtgctgtcga
     2221 tgcacggcga gcaccagtac gcggcgcgcg cgctgaaagc cggtgcctcg ggctatctca
     2281 ccaaggacag cgccgccgag cagctggtcg gcgcgatccg caagatcgcg gcaggaggcg
     2341 tgcacatcag cgaggccgcg gcagcggcgc tggttgcctc gaattcatct catgaaagcc
     2401 tgtccgaccg ggagttcgag gtcctgcgcc tgctggcgga aggcttcggc cccacccaga
     2461 tcgccgaacg gttgcatctc tcagtcaaga ccgtcagcac ccacaagacg cgcatcctgc
     2521 agaagctcaa tctcggcagc accgccgagc tggtgcgcta cgcgctcgag cacaaactcc
     2581 tgcagtaggt aggaatcgtc ctacgccgcg gaccggcgtc gtcccacggt ccgattcagc
     2641 cgccgccgat gttgcgccgc gttgcggcgc gccacactgc cggcgtgcaa atctacgtcg
     2701 tcgaagactc ccgcgcggtg cgcgagcgcc tgatcgagat gttcagcctg gtcccgcacg
     2761 tccgcgtcgt cggccacgcc gccacggcgg aggcggcgat ccacgacatc ctcgaaagca
     2821 agcccgacct cgtggtgctc gacttgcatc tcgccgaagg cagcggcttc gacgtgctgc
     2881 gcgcggtgca cccccgcgcg cccgggatcg agttctacat gctgtccaat ttctccgccg
     2941 atccctaccg gcagctcgcc gagcggctgg gggcgctcgg gttcttcgac aagagcaagg
     3001 aattcgagcg cgtgagagac gtcgtcgcca agcgcgcccg gtgccatgca aagcgtgccg
     3061 accagacact aagttgaaac gaagggaacc gccatgccag tcgtcgccgc actcaaacca
     3121 aagccgagga cgttcttcat gagccagccc gaagcgccgc gcaagttcga ggtcacctgc
     3181 tcgagctgca acctgcgcga gatgtgcctt cctggcggcg tatgcctcga ggacctcgag
     3241 cgtgtcgaga acatcgtcta cgcccggcgc cgcatcaagc gcggcgagac gctgttcaat
     3301 gctggcgacg agttcaagtc ggtgtacgcc atccgcagcg gcttcttcaa gaccagcctg
     3361 ctcgacggcg agggccgcga gcaggtgacc ggcttcttca tgggcggcga gctgctcggc
     3421 atggagggca tcgggtcggg acgctgcacc ggcaccgcca tcgccctgga ggacagtgag
     3481 gtgtgcgtga tgccgttcgc gctgatcgag gagatggcgc gcgaagtccc ggcgctgcag
     3541 cgccatctgc acgcggtgat ggcgcgcgag atcgtgcgcg accacggcgt gatgatgctg
     3601 ctcggctcga tgcgcgccga gcagcgcctc gcggcgttcc tcaccaacct gtcgaagcgc
     3661 ttcgtgcgcc gcggttactc ggcctccgac ttccacctgc gcatgacgcg cgacgagatc
     3721 ggcagctacc tagggctgaa gctcgagacc gtgagccggc tgttctccca gttccagaag
     3781 gacggcgtga tcgaggtcga gcagaagcac gtgcgcatcc tcgacagcgc cggcctcgaa
     3841 aaaattctgg cgaccagata agagacccgt gaaaacatcc ttctttgcat ggagcacggc
     3901 tgcgttgctg ctctccgcct gcggacaacg taatgatccc tacgacgtcg cgaaaatgga
     3961 cctcaagccg gtgccggcca agatctcggc ggagcgccac cagggcgcct tcgccgaggg
     4021 cgcggcgctg agtctcggcg ccgagctgaa gccgctcgat ccgtcgccgg tgaagaccgt
     4081 gcggctcgac accacccaca ggatcatcga gatcgctccc ggggtgaact tcagcgcctg
     4141 gaccttcggc aaccaggtgc ccgg
//
LOCUS       DASPXW010000397         2315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1253674, whole genome shotgun sequence.
ACCESSION   DASPXW010000397 DASPXW010000000
VERSION     DASPXW010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253674"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1170
                     /locus_tag="VEL04_09130"
     CDS             <1..1170
                     /locus_tag="VEL04_09130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYT48572.1"
                     /translation="SPAQPSRPPSMRIAFYAPLKPPTYGTPSGDRRVAGLLMDALARA
                     GQRVELVSTFRSFDGDGDAERQGALRSQGEALGRTLAAQWRDAPHAERPGLWFTYHLY
                     YKAPDWLGPVVSEALGIPYVIAEASYAPKRAGGPWAIGHEASVAAIRRAALVVCPSRD
                     DIACLEALIPGERMLHLPPFLELGPYRAAAAARDAHRARLAKAHGLDPAVLWIAVVAM
                     MRAGDKLASYRALAATLARLVDLPWRLVVVGDGPMRAEARAALESAVPGRTVFLGELK
                     NKDLAPVYAACDLTAWPAVNEAYGMALLEAQAAGLPVVSCAVRGVPEVVLDGRTGLLA
                     PVLDEAALAERIRALLLDGERRRALGRGAAAYVAAERSLEAAAARLGQALAPLAA"
     gene            1167..1745
                     /locus_tag="VEL04_09135"
     CDS             1167..1745
                     /locus_tag="VEL04_09135"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HYT48573.1"
                     /translation="MTLLALLRHADTAWSAEGRIQGRADPPLSEEGRASLAGRSLPEA
                     CRGMRVVTSPLIRCVETAALLGAPRAPRDPRLAEMDWGGWQGRRLAELRSELGAAMRD
                     NEARGLDFRPQGGESPREVMLRLQGWLAELAADAAPTLAVTHRGVIRAILAAACGWDM
                     RGPPPAKLDWSAFHLFRLAAGGRPRVERLNVK"
     gene            1742..>2315
                     /locus_tag="VEL04_09140"
     CDS             1742..>2315
                     /locus_tag="VEL04_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48574.1"
                     /translation="MKAFFYVQHLLGIGHLKRAATLARALAEDGFEVTLASGGPRVAE
                     IDLQKVKLVQLPPASAADMTFKVLLDESGLPVNHAWKARRRETLLEAWRGAAADVLLI
                     ELFPFGRRQMRFELLPLLEAARAGTRPPLVVSSVRDLIGAGQGDARRQDEMLEAAERF
                     FDRILVHGDPKLVGFERSFTHAARLGTPLHY"
BASE COUNT          287 a          845 c          853 g          330 t
ORIGIN      
        1 tcgccggcgc agcccagcag gcccccgtcc atgcggatcg cgttctacgc tccgcttaag
       61 ccgccgactt acggcacgcc ctcgggggac cgccgcgtcg ccgggctgct gatggatgcg
      121 ctcgcgcgcg ccgggcagcg cgtcgagctg gtgtcgacct tccgcagctt cgacggcgac
      181 ggcgacgcgg agcgccaggg cgcgctgcgc agccagggcg aggcgctcgg ccgcacgctc
      241 gcggcgcaat ggcgcgacgc gccgcacgcc gagcgccccg gcctctggtt cacctatcac
      301 ctgtattaca aggcgcccga ctggctcggc ccggtggtga gcgaggcgct cggcatcccg
      361 tacgtcatcg ccgaagcttc gtacgcgccc aagcgcgccg gcgggccgtg ggcgatcggg
      421 cacgaggcct cggtggccgc gatccgtcgc gcggcgctgg tggtctgccc gtcgcgcgac
      481 gacatcgcct gcctcgaggc gttgattccc ggcgagcgca tgctgcacct gccgccgttt
      541 ctcgagctcg ggccttaccg cgccgcggcc gccgcgcgcg acgcgcaccg cgcgcgcctc
      601 gcgaaggcgc atggcctcga tccggccgtg ctctggatcg cggtggtggc gatgatgcgc
      661 gccggggaca agctcgcctc gtaccgcgcg ctcgccgcga cgctcgcgcg ccttgtcgac
      721 ctgccctggc ggctggtcgt ggtgggagat ggcccgatga gagccgaagc gcgcgcagcg
      781 ctcgaaagcg ccgtgcccgg ccgcacggtg ttcctcgggg aattgaagaa caaggacctg
      841 gccccggtgt atgccgcctg cgacctcacc gcctggccgg cggtcaacga ggcctatggc
      901 atggcgctgc tcgaggcgca ggccgccggc ctgccggtgg tctcgtgcgc ggtgcgcggc
      961 gtccccgaag tggtgctcga cggcaggacc ggcctgcttg ctccggtgct cgacgaggcc
     1021 gcgctcgccg agcgcatccg tgcccttctc ctcgatgggg agcggcggcg cgcgctcggg
     1081 cgcggcgccg cggcatacgt cgccgcagaa cgcagcctcg aggccgccgc cgcgcgtctc
     1141 ggccaggccc tcgccccgct cgccgcatga cgctgcttgc gctgctgcgc cacgccgaca
     1201 cggcgtggag cgccgagggg cgtatccagg gccgcgccga cccgccgttg agcgaagagg
     1261 ggcgcgcttc gctcgccggc cgcagcctgc cggaggcctg ccgcggcatg cgtgtagtga
     1321 ccagcccgct gattcgctgc gtcgagacgg ccgcgctgct cggcgcgccg cgggcgccgc
     1381 gcgatccgcg gctcgccgag atggactggg gaggctggca ggggcggcgg ctcgccgagc
     1441 tgcgcagcga gctcggcgct gcaatgcgcg acaacgaagc ccgcggcctc gatttcaggc
     1501 cgcagggcgg cgaaagcccg cgcgaagtga tgctgcgcct gcaaggctgg ctcgccgagc
     1561 ttgcagccga cgcggcgccg acgctcgccg tgacgcatcg cggcgtgatc cgcgcgatcc
     1621 tcgccgcggc ctgcggctgg gacatgcgcg gcccgccgcc ggcgaagctc gactggagcg
     1681 cgtttcacct gttccgcctc gccgccggcg gcaggccgcg cgtcgagcgc ctgaacgtga
     1741 aatgaaggcc ttcttttacg tgcagcacct gctcggcatc gggcacctga agcgcgctgc
     1801 cacgctcgcc cgcgccctcg ctgaagacgg attcgaagtc acgctcgcga gcggaggtcc
     1861 gcgggtagcg gaaatcgatc tgcaaaaggt aaagctggtt cagcttccgc cggcgagcgc
     1921 ggcggacatg actttcaagg tgttgctgga tgaaagtggt ctgccggtga accacgcatg
     1981 gaaggcccgc cggcgggaaa cgttgctcga ggcgtggcgc ggcgccgccg ccgacgtgct
     2041 gctcatcgag ctgtttccgt tcggacgccg ccagatgcgc ttcgagctgc tgccgctcct
     2101 cgaggccgcg cgtgccggca cgcggccgcc gctcgtcgtc tcgtcggtgc gcgacctgat
     2161 cggggcgggg cagggcgatg cgcggcgcca ggacgagatg ctcgaagccg cggagcgctt
     2221 cttcgatcgc atcctggtcc acggcgaccc gaagctggtc ggcttcgagc gcagcttcac
     2281 gcatgccgcg cgcctcggga ccccgctgca ttaca
//
LOCUS       DASPXW010000398         2356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_995955, whole genome shotgun sequence.
ACCESSION   DASPXW010000398 DASPXW010000000
VERSION     DASPXW010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995955"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VEL04_09145"
     CDS             complement(<1..180)
                     /locus_tag="VEL04_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HYT48575.1"
                     /translation="MKSTLTPGLSFSKSILVDEARCIGFMGKEGMVYATPRMVSDVEY
                     ACRDFLLEHLDAGEDS"
     gene            complement(441..905)
                     /locus_tag="VEL04_09150"
     CDS             complement(441..905)
                     /locus_tag="VEL04_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48576.1"
                     /translation="MCFNTNILAAAHRSAMRTLALLAVTVFALALVPATYADERARGP
                     NGGQIKDAGKYHLELVVKENGLTVYVIGAKDAKVATKGATSSATVLAGKNTSSIKLEP
                     SGENALVGSGTFQPAPDMKVVVSVTLLGQAPIQARFAPLETPKPSAKAAAKQ"
     gene            complement(926..>2356)
                     /locus_tag="VEL04_09155"
     CDS             complement(926..>2356)
                     /locus_tag="VEL04_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HYT48577.1"
                     /translation="PFNEGTLTINLLLNPGTSLGESSRIGTQAELLIRQVPEVLQAGR
                     RTGRAELDEHAEGVHYSEIDVELKRSQRSRDAVLQDIRKRLSVLPAVVNVGQPISHRL
                     DHILSGVNAQIALKVYGDDLDTLRGLAAQLRDRLATIPGITDLQIEKAVLIPQLKVRL
                     DYEKAAAYGVTPSAVLKALETLLGGEKMAQVIEGSRRFDLVVRLPDSARGPRALEQLL
                     IETPGGRVALSRIAEIEDGDGPNQISRDNTRRRIAISANSDGTDLAAIVARIRAELAA
                     NPLPAGYFTSLEGQFQTQEEATRLIALLAVMSVLLIFIVLYTRYRSTALSLMIMGNIP
                     LALVGSVIALWIAGQPLSVASMIGFFALAGISTRNGILKVSHYINLCALEGEQFGKPM
                     IVRGSLERLTPVFMTALVAAFAVVPLMLAADEPGKEILHPVAVVLFGGLVSSTILDTI
                     ITPAMFWLWGEKPLKRLLEEHAQHAF"
BASE COUNT          396 a          760 c          763 g          437 t
ORIGIN      
        1 cgaatcctcg ccggcatcga ggtgctcgag gaggaaatcg cggcaggcgt actcgacatc
       61 gctcaccatg cgcggcgtag cgtagaccat gccttccttg cccatgaagc cgatgcagcg
      121 cgcttcgtcc acaaggatag atttcgagaa cgacaatccc ggcgttaatg tgcttttcat
      181 cggccgccat tgtagccgtg catcgcgaga cctcatctcg cgtttcggat catctcggca
      241 cgcgcctcag tggccgccca ggccgcggcc ggtgaggtgc aaaatcgcaa gatgcagaat
      301 caggacgatg acaatgattc catgcacatg cgctcggcgc agtgtgatgc tccatgccgg
      361 gatgcttctg catcatctcg gtatgctgca tcagctcttc catcgtgccg agcaccatct
      421 cgtgcctctg cgcgcccagc tcattgcttg gccgctgctt tggctgaagg tttcggtgtt
      481 tcgagcggcg cgaaccgggc ctggatcggc gcctggccca gcagcgtgac cgacactacg
      541 accttcatgt ccggtgccgg ctggaaggtg ccgctgccga cgagcgcatt ctcgccgctc
      601 ggctcgagct tgatgctgga ggtgttcttg ccagcgagca cagttgcgct gctggtcgcg
      661 cctttggtcg cgactttcgc gtccttggcc ccaatcacat agaccgtcaa cccgttctcc
      721 ttgacgacca gttcgagatg atacttacct gcgtccttga tctgaccgcc gttaggacct
      781 ctagcccgct cgtcagcgta agtcgctggc acgagtgcca gcgcaaaaac ggtgacggcg
      841 agcagtgcga gcgtgcgcat cgcagagcgg tgcgccgcgg caaggatgtt ggtgttaaag
      901 cacatggctt ttctcctttc gcagattaga acgcgtgctg cgcatgctcc tcgagcaggc
      961 gcttgagcgg tttttcgccc cacagccaga acatcgccgg agtgatgatg gtgtcgagga
     1021 tggtggagct caccagcccg ccgaacagca ccaccgccac ggggtgcagg atttccttgc
     1081 ccggctcgtc cgccgcgagc atcagcggga ccaccgcgaa cgccgccacc agcgccgtca
     1141 tgaacaccgg cgtcaatcgt tcgagcgagc cgcgcacgat catcggcttg ccgaattgct
     1201 cgccctccag ggcgcacagg ttgatgtagt ggctcacctt cagaattccg ttgcgcgtgc
     1261 tgatgccggc cagcgcgaag aagccgatca tcgaggcgac cgacagcggc tggccggcaa
     1321 tccagagcgc gatgacgctg ccgaccagcg cgagcggaat attgcccatg atcatcagcg
     1381 acagcgcggt ggaacggtag cgggtgtaga gcacgatgaa gatcagcagc accgacatca
     1441 cggcgagcag ggcgatcagc cgtgtcgcct cctcctgcgt ctggaactga ccctccaggc
     1501 tggtgaaata gccggcgggc agcgggttgg cggcgagctc agcgcggatg cgcgctacga
     1561 tcgcggcgag gtcggtgccg tcggagttgg ccgagatcgc gatgcgccgg cgcgtgttgt
     1621 cgcgcgagat ctggttgggg ccgtcgccgt cctcgatctc ggcgatgcgc gagagcgcga
     1681 cgcggccgcc cggagtctcg atcagaagct gctccagcgc ccgcgggccg cgcgcggagt
     1741 cgggcaggcg caccaccaga tcgaagcggc ggctgccttc gatcacctgc gccattttct
     1801 ctccgcccag cagcgtctcg agcgccttca gcaccgcgga cggggtgacg ccgtaggcgg
     1861 ccgctttctc gtaatcgagg cgcactttga gctgcgggat caataccgcc ttctcgatct
     1921 gcagatccgt gatgccgggg atcgtcgcca gccggtcgcg caactgcgcg gcgaggccgc
     1981 gcagcgtgtc gagatcatca ccgtagacct tgagcgcgat ctgcgcgttc acccccgaga
     2041 gaatgtggtc gagccggtgc gagatcggct gcccgacgtt gaccactgcg ggcagcacgc
     2101 tcaagcgctt gcggatgtcc tgcagcacgg cgtcgcggct gcgctgcgag cgcttcagct
     2161 cgacgtcgat ctcggagtaa tgcacgcctt ccgcatgctc gtccagctcc gcgcgcccgg
     2221 tgcggcgccc ggcctgaagg acttccggca cctgcctgat caggagctcc gcctgggtgc
     2281 cgatgcggct cgattcgccg agcgaggttc ccgggttgag cagcaggttg atggtgagcg
     2341 tgccctcgtt gaacgg
//
LOCUS       DASPXW010000399         2015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_240473, whole genome shotgun sequence.
ACCESSION   DASPXW010000399 DASPXW010000000
VERSION     DASPXW010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240473"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="VEL04_09160"
     CDS             complement(<1..370)
                     /locus_tag="VEL04_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HYT48578.1"
                     /translation="MARPRFVHLRLHSEYSVNDGIVRIDDAVAHAAADEMPALALTDT
                     ANLFGMVKFYGAARTAGVKPVLGADCWLQNDADREKPFRLLLLCASRAGYLRLCDLLS
                     RAWLKNQHRGRAEIASSWLEA"
     gene            427..1290
                     /locus_tag="VEL04_09165"
     CDS             427..1290
                     /locus_tag="VEL04_09165"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYT48579.1"
                     /translation="MRPALAALLVGATCIALSPIFVRVSEAGPTATAFWRVALAVPAL
                     WLLCPIFKKTPASAAAGWPLFLAAGAAFAGDLAFWHSSIKLTSVANSTLLANLAALFV
                     TLAAWVFLRQRPSGPFLAGLAAALAGVALLVHTSLEFSPTGLVGDALGVVTAMFYAGY
                     ILAVKRLRDRGESTLRLMAATSTITALFLFPIALASGEQMLPATAFGWWMLLGLALVS
                     HAAGQGLIAYALAHLPAPFSSVSLLFQPVMAAAFAWALLSEPLVPLQIAGAGVVLAGI
                     YLAGRGSKAGE"
     gene            1321..1740
                     /locus_tag="VEL04_09170"
     CDS             1321..1740
                     /locus_tag="VEL04_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48580.1"
                     /translation="MAVKKKAKPKRRKTPAKRSPIRKKAVKLARKPTRKLPVRRAPPK
                     AATPPSPPPPPAPGERIGVVTHYYGHLSVAIIKLESATLRVGDTIHIRGHTSDFSQRV
                     ESLQIGHAPVAEAGPQDDFGLKVTEHVRENDVVYKVK"
     gene            complement(1748..>2015)
                     /locus_tag="VEL04_09175"
     CDS             complement(1748..>2015)
                     /locus_tag="VEL04_09175"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HYT48581.1"
                     /translation="RMLPAGTKAVIRKAAWPRPQLFHRLQQAGGVAEGEMLRVFNCGI
                     GMVIALARDQVASASMLLRREGELVYEIGTIEQASGAGPECLIV"
BASE COUNT          322 a          694 c          652 g          347 t
ORIGIN      
        1 cggcctcgag ccagctcgag gcgatttcgg ccctgccgcg gtgctggttc ttcagccacg
       61 cccgcgacag aaggtcgcag agcctgaggt agccggcgcg cgacgcgcac aacagcagga
      121 gcctgaaggg cttctcgcgg tcggcatcgt tctgcaacca gcaatcggca cccaggaccg
      181 gcttgacgcc ggcggtccgg gcggcgccgt agaacttcac catgccgaac aggttggccg
      241 tatcggtaag cgccagcgcc ggcatttcgt ccgcggcggc gtgcgccacc gcatcgtcaa
      301 tgcgcactat gccgtcgttc accgagtatt cactgtgcag cctgaggtga acgaaacgcg
      361 gccttgccat gtcaaaaaat tctaccggaa agggcatcac gaaacctcaa gtaacatcgg
      421 cgggccatga ggcccgcgct cgctgcgctt ctcgtcggcg cgacctgcat tgccctgtcg
      481 ccgattttcg tgcgcgtctc ggaagccggc cccaccgcga cagccttctg gcgcgtcgcg
      541 ctcgccgttc ccgcgctctg gctgctttgt ccgatcttca aaaaaacgcc cgcttcggcg
      601 gcagcaggct ggccgctttt cctggccgcc ggggccgcgt tcgccggcga cctcgccttc
      661 tggcatagct cgatcaagct gacgtcggtc gccaattcga cgctgctcgc caatctcgct
      721 gcgctcttcg tcacgcttgc ggcgtgggtg tttctgcgcc agcggccgag cgggccgttc
      781 cttgccggcc tcgcagccgc gcttgcgggc gtggcgctgc tcgtgcacac cagcctggaa
      841 ttctcgccga ccggcctggt cggagatgcg ctgggcgtcg tgacggcgat gttttatgcc
      901 ggctacatcc tcgcggtgaa gcgcttgcgc gatcgcggcg agagcacgct gcgcctgatg
      961 gcggcgacca gcacgataac cgcgcttttc ctcttcccca tcgcgctcgc ctcgggcgag
     1021 cagatgcttc cggcgactgc gttcggctgg tggatgctgc tcgggctggc gctggtctcg
     1081 catgccgccg gacagggtct gatcgcgtat gcgctcgcgc acctgccggc gccgttttcc
     1141 tcggtcagcc tgctgttcca gccggtaatg gcggcggcct tcgcctgggc gctgctgtcc
     1201 gagccgctcg tgccgctgca gatcgcgggc gcaggcgtgg ttttggccgg gatttatctt
     1261 gcgggccgcg gctccaaagc gggagaatga tccgcgggca atcccgcgag gagacaacac
     1321 atggcggtca agaaaaaggc caagccgaag cgcaggaaga caccagccaa gcggtcgccg
     1381 attcgcaaga aggccgtgaa gctcgcgagg aagccgacgc ggaaattacc ggtacggcgc
     1441 gcgccgccaa aagccgcgac gcccccgagc ccgccgccgc cacccgcgcc gggggagcgt
     1501 ataggcgtcg tgacccacta ttacggccat ctctcggtcg ccatcatcaa gctggaatcg
     1561 gcaaccctgc gcgtcggcga caccatccat atccgggggc acaccagcga cttcagccag
     1621 cgggtcgagt cgctgcagat cggccatgcg ccggtcgccg aagccggccc gcaggacgat
     1681 ttcgggctga aggtgaccga gcacgtgcgc gagaacgacg tcgtctacaa agtgaaataa
     1741 ccggggtcta tacgataagg cattccggcc cggcgccgga ggcctgctcg atcgtgccga
     1801 tctcgtagac cagctcgccc tccctgcgca agagcatgct agcgctggcg acttggtcgc
     1861 gcgcgagcgc gatcaccatg ccgatgccgc aattgaagac gcggagcatc tcgccctccg
     1921 cgaccccgcc ggcctgctgc agccgatgga agagctgcgg ccgcggccac gccgcttttc
     1981 ttattaccgc cttggttccg gcgggaagca tccgc
//
LOCUS       DASPXW010000400         2532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1879231, whole genome shotgun sequence.
ACCESSION   DASPXW010000400 DASPXW010000000
VERSION     DASPXW010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1879231"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2248)
                     /gene="gyrB"
                     /locus_tag="VEL04_09180"
     CDS             complement(<1..2248)
                     /gene="gyrB"
                     /locus_tag="VEL04_09180"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016343927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HYT48582.1"
                     /translation="MGEPERQLPDANGYGSDAIKVLKGLDAVRKRPGMYIGDTADGTG
                     LHHMVFEVVDNSVDEALAGFCDEITVTVHTDNSISVLDNGRGIPTGIKEDDELKRSAA
                     EIVMTELHAGGKFDSNSYKVSGGLHGVGVSVVNALSEWLRLTIWRDGRVHHMEFRKGV
                     PVEPLKITGASERRGTEVHFLASKEIFGNVDYHYDILARRLRELSFLNNGVRIVLIDQ
                     RAGKEENFAFSGGVKGFVEYMNRAKNVLHPNVFVGLGVKDGITVEVAMQWNDSYQESV
                     LCFTNNIPQKDGGTHMTGLRAAMTRVLNKYIEETELAKKAKVETTGDDMREGLACVLS
                     VKVPEPKFSSQTKEKLVSSEVRPVVEEIVAERLRGFLAERPADARLLTSKIVDAARAR
                     EAARKAREMTRRKGVLDSMGLPGKLADCQERDPAKCELYLVEGDSAGGSAKQGRDRRF
                     QAILPLKGKILNVERARFDKLLSSTEIATLITALGCGIGKEEYNAEKLRYHRIIIMTD
                     ADVDGSHIRTLLLTFFYRQMPELVERGYIYIAQPPLYKAKLGKEERYLKDEHELNEFM
                     LRQALIDAALHPALGREPIAGEALGQLAKSYLVSEAVIERVSRLIDREVLQAMVRGVQ
                     VDLSRRDTAEASAEALEAAIGGNGIAVAPHFDAKTERYQVRIERTRHGNVRMSTIDTE
                     FVHSGDYTQIRQTAALLRGLIGEGAYMQRGDKKQPVREFSEAMQWLLGEVQKSMSLQR
                     YKGLGEMNP"
     gene            complement(2250..>2532)
                     /locus_tag="VEL04_09185"
     CDS             complement(2250..>2532)
                     /locus_tag="VEL04_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HYT48583.1"
                     /translation="SNEKFRGVRWVLTDGSLKIVSSNAELEEAHEEIEVKYAGDALDI
                     GFNVNYLLDVLNNVAGNEVECAFGDASSSALISYASEKDFRYVVMPMRI"
BASE COUNT          421 a          830 c          758 g          523 t
ORIGIN      
        1 cgggattcat ttcgccgagt cccttgtagc gttgcaggct catcgacttt tgcacttcgc
       61 cgagcagcca ctgcatcgct tcagagaatt cgcgcaccgg ctgcttcttg tcgccgcgct
      121 gcatgtacgc gccctcgcca atcagcccgc gcagcagcgc tgccgtctgc cggatctgcg
      181 tgtaatcgcc cgagtgcacg aattcggtgt cgatggtgct catgcgcacg ttgccgtgtc
      241 gggtgcgctc gatgcgcacc tggtagcgct ccgtcttggc atcaaaatgc ggcgccaccg
      301 cgatgccgtt gccgccgatc gcggcctcga gcgcctcggc ggaggcttcg gccgtatcgc
      361 gccgtgacag gtcgacctgt accccgcgca ccatcgcctg caggacctcg cgatcgatga
      421 gccgcgaaac gcgctcgatc accgcctcgg agaccaggta agacttggcg agttgcccga
      481 gcgcctcgcc ggcgatcggc tcgcgcccga gcgccgggtg caaggcggcg tcgatcagcg
      541 cctgcctgag catgaactcg ttcagctcgt gttcgtcctt gagatagcgt tcctccttgc
      601 caagcttcgc tttatacagc ggcggttgcg cgatgtagat atagccgcgc tccaccagtt
      661 ccggcatctg ccggtagaag aacgtgagca gcagggtgcg gatgtgcgac ccgtcgacat
      721 ccgcgtcggt catgatgatg atgcggtggt agcgcagctt ctcggcgttg tactcctcct
      781 tcccgatgcc gcagcccagc gcggtgatca gcgtcgcgat ctccgtcgaa gacagcagct
      841 tgtcgaagcg cgcgcgctcg acattcagga tcttgccttt caggggcagg atcgcctgga
      901 aacgcctatc gcgcccctgc ttggcggagc ctcccgccga gtcgccctcg accagataga
      961 gctcgcattt agcgggatcg cgttcctggc agtcggcgag cttgccaggc agacccatcg
     1021 agtcgagcac gcccttgcgg cgcgtcattt cgcgcgcctt gcgggctgcc tcccgggcgc
     1081 gcgccgcatc gacgatcttc gaagtgagca accgagcgtc cgcgggacgt tcggcgagga
     1141 atccgcgcag ccgctcggcg acgatttcct cgaccaccgg gcgaacctcg gaggacacga
     1201 gcttttcctt ggtctgggag gagaacttcg gctcggggac cttcactgaa agcacgcacg
     1261 cgagtccctc gcgcatgtcg tcgcccgtgg tttcgacttt cgccttcttg gcgagctcgg
     1321 tctcttcgat gtacttgttg agcacgcggg tcattgccgc gcgcaatccg gtcatatgcg
     1381 tgccgccgtc cttttgcggg atattgttgg tgaagcacag cacgctctcc tggtatgaat
     1441 cgttccactg catcgcgacc tcgaccgtga tgccatcctt gacgccgagg ccgacgaata
     1501 cgttcgggtg caggacattc ttggctcggt tcatgtactc cacgaacccc ttgacgccgc
     1561 cggagaacgc gaagttctcc tccttgccgg cgcgctggtc gatcagcacg atcctgacgc
     1621 cgttgttgag aaaggagagc tcgcgcagcc gccgcgccag gatgtcgtag tggtagtcga
     1681 cgttgccgaa gatctccttc gatgcaagga agtgcacctc ggtaccacgc cgctcgctgg
     1741 cgccggtgat cttgagtggc tcgaccggga ctcccttgcg gaactccatg tgatgcaccc
     1801 ggccatcgcg ccagatggtg agccgaagcc actccgaaag cgcattcacg actgacacac
     1861 cgacgccatg caagccgccg gacaccttgt agctgttgga gtcgaacttg cctccggcgt
     1921 gcagttcggt cattacgatc tcggccgccg agcgcttgag ctcgtcgtcc tccttgatac
     1981 cggtgggaat gccgcggccg ttatcgagca ccgagatcga gttgtccgta tgcacggtaa
     2041 ccgtgatttc gtcgcagaac cctgcgagcg cctcatccac cgagttgtca actacctcga
     2101 acaccatgtg atgcagcccg gtgccatcgg cggtatcgcc gatgtacatg ccgggacgct
     2161 tgcgcaccgc atcgagtccc ttcaggactt tgatcgcatc cgagccgtag ccgtttgcgt
     2221 cggggagctg tcgctcgggt tcgcccaccc tagatcctca tcggcatgac aacgtaacgg
     2281 aagtccttct cggaggcata gctgatcagc gcgctcgacg aggcatcgcc gaaggcgcac
     2341 tcgacttcat tccccgcgac attattcagc acgtcgagca ggtagttgac attgaatccg
     2401 atgtcgagcg catcgccggc atacttgacc tcgatctcct cgtgcgcctc ttccagctcg
     2461 gcattggagg agacgatctt taggctgccg tcggtcagta cccatcgcac gccgcgaaat
     2521 ttctcgttcg ac
//
LOCUS       DASPXW010000401         2413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_492153, whole genome shotgun sequence.
ACCESSION   DASPXW010000401 DASPXW010000000
VERSION     DASPXW010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492153"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /gene="nuoI"
                     /locus_tag="VEL04_09190"
     CDS             complement(<1..305)
                     /gene="nuoI"
                     /locus_tag="VEL04_09190"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HYT48584.1"
                     /translation="MTRVLEFFKSFLLIELLRGLALTGRHLFARKITIQFPEEKTPAS
                     PRFRGLHALRRYPNGEERCIACKLCEAVCPALAIHIESEQRDDGTRRTTRYDIDLTK"
     gene            complement(302..1393)
                     /gene="nuoH"
                     /locus_tag="VEL04_09195"
     CDS             complement(302..1393)
                     /gene="nuoH"
                     /locus_tag="VEL04_09195"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HYT48585.1"
                     /translation="MLQTLLSFSEARLGAVWGPAVYQTVTSLAFIVAIVAPLFLCVAY
                     LTLWERKLIGWIQIRIGPNRVGPAGLLQPIADGVKLLFKEIILPTNANRGLFLLAPVL
                     ILAPALAGWAVIPFSPDVVLADINAGLLYILALSSMGVYGVIVAGWASNSKYAFLGAM
                     RSAAQMVSYELAMGFALVVVLMVSGSLNLSDIVNAQGRGVFASKGLNFLSWNWLPLLP
                     MLIVYFVSGVAETNRAPFDVVEGESEIVAGHMVEYSGMTFALFFMAEYMNMLLISTLT
                     AVMFFGGWQAPLNAYLFTEVIPFKWPFTWLFIKIFLIVSMFLWFRATFPRYRFDQIMR
                     LGWKVFIPLTLVWIAVVAIWMQTPWNIWR"
     gene            complement(1394..>2413)
                     /locus_tag="VEL04_09200"
     CDS             complement(1394..>2413)
                     /locus_tag="VEL04_09200"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HYT48586.1"
                     /translation="LHSVDDDWLMPVKSRKIVAPSEMVSAIASFREILAGGKNAAVLL
                     GNFAQQHPHAAQLHAAAQALGVKLGVLAEAANSVGGYVAGLPVGGGLKAALQKNAIVV
                     LNAEPGLDCADPQTVGAALGQAQCVVNLTAYKTGLEFADVLLPIAPFSETPGTFVSME
                     GRAQSFHATVNPLGEARPAWKVLRVLGTLRGTPGFEFDTIEQVRAACLQGRDIASLLS
                     NSINLAEQKKQITASGIQRIADVPIYFADPLVRRSAPLQKTKDAEQPKAWMNAKLMQK
                     LGVTAGQRVLIKQNRGTATLAAALDDKLPDDCVRVAAAHASTAALGPMFGTLSVEKVS
                     VQQAA"
BASE COUNT          460 a          791 c          759 g          403 t
ORIGIN      
        1 catttcgtca ggtcgatgtc gtagcgcgtg gtgcgccgcg tgccgtcgtc gcgctgctcg
       61 gattcgatgt gtatggcgag cgccgggcac accgcctcgc acagcttgca ggcgatgcag
      121 cgctcttcgc cgttcggata acggcgcagc gcgtgcaggc cgcggaagcg cgggctggcg
      181 ggcgtcttct cctccgggaa ctggatcgtg atcttgcgcg cgaacaggtg tcttcctgtc
      241 aacgccaggc ctcgcagaag ctcgatcaat aaaaacgact tgaaaaactc gagcacgcgg
      301 gtcatcgcca gatgttccag ggagtctgca tccagattgc gaccaccgcg atccacacca
      361 gggtgagcgg gatgaacacc ttccagccca ggcgcatgat ctggtcgaag cggtagcgcg
      421 ggaacgtggc cctgaaccac aggaacatcg acacgatgag gaatatcttg atgaataacc
      481 acgtgaaagg ccacttgaag ggaatcacct cggtaaataa atacgcgttc agcggcgcct
      541 gccagccgcc gaagaacatc actgcggtca gcgtcgaaat cagcagcatg ttcatgtact
      601 cggccatgaa gaacagggcg aaggtcatgc ccgagtactc gaccatgtgg ccggcgacga
      661 tttccgactc gccctccacc acgtcgaagg gcgcgcggtt ggtttcggcg accccggata
      721 caaaatacac aatcagcatc ggcagcaggg gcagccagtt ccacgacagg aagttcagcc
      781 ccttcgaggc gaacacgccg cgtccctgcg cgttgacgat gtccgagagg ttgaggctgc
      841 ccgagaccat cagcaccacc accagcgcga agcccatcgc cagctcgtag gacaccatct
      901 gcgccgccga gcgcatggcg ccgaggaagg cgtacttcga gttcgacgcc cagccggcga
      961 cgatcacgcc gtacaccccc atcgagctca acgccaggat atagagcagg ccggcgttga
     1021 tgtcggcgag caccacgtcg ggcgagaacg ggatcaccgc ccagccggcg agcgccggcg
     1081 ccaggatcag caccggcgcg agcaggaaca ggccccggtt ggcgttggtc ggaaggatga
     1141 tctccttgaa gagcagcttg acgccgtcgg cgatcggctg cagcagcccc gccggcccga
     1201 cgcggttcgg gccgatgcgg atctggatcc agccgatcag cttgcgctcc cagagcgtga
     1261 ggtaggcgac gcacaggaaa agcggcgcca cgatcgcgac gatgaacgcg aggctggtca
     1321 cggtctggta taccgccggc ccccagaccg cgccgagtct cgcctcgctg aacgaaagga
     1381 gggtctgcag catctaggcg gcctgctgca ccgaaacctt ctccacgctc agcgtgccga
     1441 acatcgggcc gagcgccgcc gtcgaagcgt gcgcggcggc gacccgcacg cagtcgtcgg
     1501 gcagcttgtc gtcgagcgcg gctgccagtg ttgcagttcc tcgattttgt tttatcaaga
     1561 cacgttgtcc ggcagtgacc ccgagctttt gcatgagctt cgcgttcatc caggccttcg
     1621 gctgctcggc gtctttcgtc ttctgcagcg gcgcggagcg ccgtaccagc ggatccgcga
     1681 agtagatcgg cacatcggcg attctttgaa taccgctggc ggtgatctgc tttttttgct
     1741 ccgcaagatt gatgctgttg gaaagcagcg aagcgatatc cctgccctgc aggcaagccg
     1801 ctctcacctg ctcgatggtg tcgaactcga agcccggcgt gccacgcagc gtgccgagca
     1861 cgcgcagcac cttccaggcc ggccgcgcct cgccaagcgg attcaccgtg gcatggaagc
     1921 tctgcgcgcg cccttccatg ctgacgaagg tgcccggcgt ctcgctgaac ggcgcgatcg
     1981 gcagcagcac gtcggcgaac tcgaggccgg tcttgtaggc cgtcagattg acgacgcact
     2041 gcgcctgtcc gagcgccgcg ccgaccgtct gcggatcggc gcagtcgagc ccgggttccg
     2101 cattcaggac cacgatggca ttcttctgca acgccgcctt caggccgccg cccacgggca
     2161 gcccggccac gtagccgccg accgagttcg cggcctcggc gagaaccccc agcttcacgc
     2221 ccagcgcctg cgccgcggcg tggagttgcg ccgcgtgcgg atgctgctgc gcgaagttgc
     2281 cgagcaggac ggccgcgttt ttaccgccag caagaatttc cctgaacgaa gcaatcgcag
     2341 aaaccatttc tgaaggggcg acaatcttcc tgctcttcac cggcatcagc cagtcgtcgt
     2401 cgaccgagtg cag
//
LOCUS       DASPXW010000402         4677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1255220, whole genome shotgun sequence.
ACCESSION   DASPXW010000402 DASPXW010000000
VERSION     DASPXW010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4677
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1255220"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /gene="hisF"
                     /locus_tag="VEL04_09205"
     CDS             complement(<1..737)
                     /gene="hisF"
                     /locus_tag="VEL04_09205"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HYT48587.1"
                     /translation="MPLAKRVIPCLDVTAGRVVKGVNFVGLRDAGDPVEIAARYDGEG
                     ADELCFLDITASSDERDILLNVIEAVAERVFIPLTVGGGVRRVEDVRRLLNAGADKIS
                     INTAALQDPELVRAASGIVGNQCIVVAIDAKRHSTGWEVYTHGGRKPTGVDAVAWARR
                     MEAAGAGEILLTSMDRDGTRDGFDLPLTRAVSEAVGVPVIASGGAGNLEHLAQAVLEG
                     KADAVLAASIFHFGEYTVGEAKQHLKAR"
     gene            complement(762..1511)
                     /gene="hisA"
                     /locus_tag="VEL04_09210"
     CDS             complement(762..1511)
                     /gene="hisA"
                     /locus_tag="VEL04_09210"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HYT48588.1"
                     /translation="MLIIPAIDLKDGRCVRLQQGSMDSATVFSDDPVAMARHWAGEGA
                     KRLHIVDLNGAVAGRPKNEKVIRDMIAAVGGRLQIQLGGGIRDLDTIESYLDAGVTYI
                     VVGTAAVKNPGFLSDACYAFPGHVIAGLDAKEGKVAVEGWSKMTGHDVVDLAKKFEEY
                     GIEALVYTDIGRDGMLTGINIEATLRLAQATKTPIIASGGLNSVKDVQTVCEKLVPEG
                     VIGAIAGRALYEGKLDLKKAQAAADKALAKE"
     gene            complement(1545..2156)
                     /gene="hisH"
                     /locus_tag="VEL04_09215"
     CDS             complement(1545..2156)
                     /gene="hisH"
                     /locus_tag="VEL04_09215"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HYT48589.1"
                     /translation="MKIAVVDYGMGNLRSVSKALERVAPQAEVLVTADAEAIRAADRV
                     VVPGQGAMPDCMRQLGASGARQAVVEAASDKPFLGICIGLQMLFERGEEGDTAGLGLL
                     PGSVPRLRVAGLKIPHMGWNEVLQERSHALWHGIPDRSRFYFVHSYYPAPSNRALAAA
                     SCVYGAPFTCAVARDNIFAVQFHPEKSQSAGLQLLSNFVRWRP"
     gene            complement(2153..2746)
                     /gene="hisB"
                     /locus_tag="VEL04_09220"
     CDS             complement(2153..2746)
                     /gene="hisB"
                     /locus_tag="VEL04_09220"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HYT48590.1"
                     /translation="MRTAEVTRSTKETQIRVRVDLDGTGRARLVTGLPFLEHMLDQVA
                     RHAMLDLDIEAKGDLHIDAHHTVEDIGITLGQAFAKAIGDKAGVRRFGHAYVPLDEAL
                     SRVVIDFSGRPGLEYHVKFTRALIGEFDVDLVHEFFQGFANHAQATLHIDNLRGDNAH
                     HQCETMFKAFARALRMAAERDPRAQGGIPSTKGSLQG"
     gene            complement(2743..3804)
                     /gene="hisC"
                     /locus_tag="VEL04_09225"
     CDS             complement(2743..3804)
                     /gene="hisC"
                     /locus_tag="VEL04_09225"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HYT48591.1"
                     /translation="MKPQQLIRPEIFALKAYPVPNADGMVKLDAMENPYSLPDDLRGE
                     LAKVLAGVAVNRYPVPNPQKLHQALARRMNVPQGMELLLGNGSDELIQIVTLALARPG
                     AVMMYPSPSFVMYSMNATFFGMKAVPMPLREDFSFDATAFITRMKAEHPALVFIAYPN
                     NPTGVLYPEEDVYAVIRAAPGLVVLDEAYHAFARKSFMGRLTEFPNLVVLRTVSKLGL
                     AGIRLGYLAGRPEWIEQFNKVRPPYNVGVLTQAAALFLLERLDVLEEQAARIRTDRAV
                     LGAALAALPGVKVFPSEANFLLVRVPDADRMDEGLRRQGVLVKNLNGPIMKNCLRITV
                     GTPDENRILLTAMREALQP"
     gene            complement(3801..>4677)
                     /gene="hisD"
                     /locus_tag="VEL04_09230"
     CDS             complement(3801..>4677)
                     /gene="hisD"
                     /locus_tag="VEL04_09230"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HYT48592.1"
                     /translation="KAAYPSTVLMNAVPAKVAGVRELVMVSPQPGALVLAAAALAGVD
                     RVIAIGGAQAIAALAYGTESVPRVDKIVGPGNVYVAEAKRQVFGEVGIDMLAGPSEVL
                     VISDGSTPADWVAMDLLSQAEHDEAAQAILLSPDEKYLDAVEGAMQRLIGEMSRRSVI
                     SASLKARGALMQTRDLDEACTLANRIAPEHLELAVQDPEKLLSKVRNAGAIFLGRWSS
                     EAIGDYCAGPNHVLPTAGTARFSSPLGAYDFQKRSSVIEISRQSAAELGRVAATLAEG
                     EGLTAHARSAEMRFK"
BASE COUNT          754 a         1597 c         1533 g          793 t
ORIGIN      
        1 cgcgccttca ggtgctgctt ggcttcgccc accgtgtact cgccgaaatg gaagatgctc
       61 gccgcgagca ccgcatcggc ctttccctcg agcacggcct gcgccaggtg ctcgagattg
      121 ccggcgccgc ccgaggcgat gaccggcaca ccgacggctt cggacacagc gcgcgtcagc
      181 ggcagatcga agccgtcgcg cgtgccgtcg cggtccatgc tggtgagcag gatctcgccc
      241 gcgccggccg cctccatcct gcgcgcccag gccaccgcgt cgacgccagt gggcttgcgc
      301 ccgccatggg tgtacacctc ccagccggta gagtggcgct tggcgtcgat ggcaaccacg
      361 atgcactggt tgccgacgat gccggaggcg gcacgtacca gctcgggatc ctggagggcg
      421 gcggtattga tcgagatctt gtcggctccg gcattgagca gccgcctgac atcctcgacc
      481 cggcgcacgc cgccgccgac ggtgagcggg atgaagacgc gctcggcgac cgcctcgatc
      541 acgttgagca ggatgtcgcg ctcgtccgag ctcgcggtga tgtcgagaaa gcacagctcg
      601 tcggcgccct cgccgtcgta gcgcgcggcg atctccaccg ggtcgccggc gtcgcgcagc
      661 ccgacaaaat tcacgccctt cacgactcgc cccgcagtca cgtcgaggca ggggatcacg
      721 cgctttgcca aaggcatctc aagaatcctc gagcggcatg cttattcctt ggcgagcgcc
      781 ttgtcagcgg cagcctgagc cttcttcaga tcgagcttgc cctcgtacag ggcgcgcccg
      841 gcgatcgcgc cgatgacgcc ttcgggaacc agcttttcgc acaccgtctg cacgtccttg
      901 acgctgttca accctcccga ggcgatgatc ggcgtcttgg tcgcctgcgc caggcgcagc
      961 gtcgcctcga tgttgatgcc ggtcagcatg ccgtcgcgcc cgatatcggt gtagaccagc
     1021 gcctcgatgc cgtattcctc gaacttcttc gcgaggtcga ccacgtcgtg gccggtcatc
     1081 ttggaccagc cctcgaccgc gaccttgcct tccttcgcgt cgaggccggc gatgacgtgg
     1141 ccggggaagg cgtaacaggc atccgacagg aagcccgggt tcttcaccgc agcggtgccg
     1201 accacgatgt aggtgacgcc ggcgtcgaga tagctctcga tggtgtcgag gtcgcggatg
     1261 ccgccgccga gctggatctg cagccggccg ccgaccgcgg cgatcatgtc gcggatcact
     1321 ttctcgttct tcggccggcc ggcgacggcg ccgttgaggt cgacgatgtg caggcgcttc
     1381 gcgccctcgc cggcccagtg ccgggccatc gcgacggggt cgtcggagaa caccgtggcc
     1441 gaatccatgc tgccttgctg caggcgcacg cagcgcccgt ccttcaggtc aatcgcgggg
     1501 atgatcagca cggacaatga gcgtgcgaca cgctcgagca gaaatcaggg acgccagcgc
     1561 acgaagttgg agagaagttg caggcctgcg gactggcttt tttccgggtg gaactgaacg
     1621 gcaaagatgt tatcccgggc gaccgcgcag gtaaagggcg cgccatagac gcagctcgct
     1681 gcggcaagcg cgcggttgct cggcgccggg tagtagctgt gcacgaagta gaagcggctg
     1741 cggtcaggga tgccgtgcca taacgcgtga ctgcgctcct ggagcacctc gttccagccc
     1801 atgtgcggaa ttttcagccc cgcgacccgc aggcgcggca cgctgcccgg caaaagtccc
     1861 agccccgccg tatcgccttc ttcgccacgc tcgaacagca tctgcaagcc gatgcagatg
     1921 ccgaggaaag gcttgtccga ggccgcctcg acgaccgcct gccgcgcgcc gctcgcgccg
     1981 agttggcgca tgcagtcggg catcgcgccc tgcccgggca cgaccacccg gtcggcagcg
     2041 cggatcgcct cggcatctgc ggtcaccagc acctcggcct gcggggcgac gcgctcgagc
     2101 gccttcgaca ccgagcgcag gttccccatg ccgtaatcga cgaccgcgat cttcaaccct
     2161 ggagcgaccc cttggtcgag ggaatgccgc cttgcgcgcg cggatcgcgt tcggcggcca
     2221 tgcgcagcgc ccgcgcgaag gccttgaaca tggtttcgca ctggtggtgg gcgttgtcgc
     2281 cgcgcaggtt gtcgatgtgc agggtcgcct gggcgtgatt ggcaaacccc tggaaaaatt
     2341 cgtgcaccag gtcgacgtcg aactcgccga tcagcgcgcg cgtgaacttg acgtgatact
     2401 cgagccccgg gcgccccgag aagtcgatca ccacgcgcga cagcgcctcg tcgagcggca
     2461 cataggcatg gccgaagcgg cgcacgccgg ccttgtcgcc gatcgccttg gcgaacgcct
     2521 ggccgagcgt gatgccgatg tcctcgaccg tgtggtgcgc gtcgatgtgc aggtcgccct
     2581 tcgcctcgat gtcgagatcg agcatggcgt ggcgcgccac ctggtcgagc atgtgctcga
     2641 ggaacggcag cccggtgacg agccgtgcgc gacccgtgcc gtcgaggtcg accctgaccc
     2701 ggatctgggt ctccttggtg ctgcgggtga cttcggccgt cctcatggtt gcagcgcctc
     2761 gcgcattgcc gtgagcagta tacggttttc gtctggcgtg ccgacagtga tgcgcaagca
     2821 gttcttcatg atggggccgt tgagattctt caccagcacg ccctggcggc gcagcccctc
     2881 gtccatgcgg tcggcgtccg gcacccgaac cagcaaaaaa tttgcctccg aggggaacac
     2941 cttcaccccc ggcagcgcgg cgagcgcggc tcccagcacc gcgcggtcgg tacgaatgcg
     3001 cgcggcctgt tcctcgagca cgtctagccg ctcgagcagg aacaatgcgg ccgcctgcgt
     3061 cagcacgccg acgttatacg gcggcctgac cttgttgaac tgctcgatcc actcgggccg
     3121 gccggcgaga taccccagcc ggataccggc gaggccgagc ttcgagaccg tgcgcagcac
     3181 caccaggttc ggaaactcgg tgagccgtcc catgaagctc ttgcgcgcga aggcgtggta
     3241 agcctcgtcc agcaccacca gtcccggcgc agctcgtatg acggcgtaga cgtcctcttc
     3301 cggatagagc accccggtcg gattgttcgg ataggcgatg aacaccagcg cggggtgctc
     3361 cgccttcata cgcgtgatga aagccgtggc gtcgaacgag aagtcctcgc gtaacggcat
     3421 cggcacagct ttcatgccga agaaggtcgc gttcatcgag tacatgacga agctcggcga
     3481 cgggtacatc atcactgctc ccggacgcgc cagcgccagg gtcacgatct ggatcagctc
     3541 gtccgagccg ttgccaagca gcagctccat gccctgcggc acgttcatcc tgcgcgccag
     3601 ggcctggtgc agcttctgcg gattcggcac gggataacgg ttcacagcga cgcccgccag
     3661 caccttcgcg agctcgccac gcaggtcgtc gggcagcgag taggggttct ccatcgcgtc
     3721 gagcttcacc atgccatctg cattcggcac gggataggcc ttcagcgcga agatctcggg
     3781 ccggatgagc tgttgcggtt tcatttgaag cgcatctccg ccgatctggc gtgcgccgtc
     3841 aggccctcgc cctcggcaag cgttgcggcg acgcggccca gctccgcagc ggactgcctg
     3901 gaaatctcga tgacgcttga gcgcttctga aaatcgtagg ccccgagagg cgaggagaac
     3961 cgcgcggtgc ccgccgtcgg cagcacgtgg ttcggcccgg cgcagtaatc gccgatcgcc
     4021 tccgagctcc agcgcccgag gaagatcgca cccgcgtttc tcaccttgga tagaagtttt
     4081 tcaggatcct gcactgcgag ctcgaggtgc tcgggggcga tacggttcgc gagcgtgcag
     4141 gcctcgtcca ggtcgcgcgt ctgcatgagc gcgccgcgcg ctttgagcga tgcggaaatg
     4201 acgcttcttc tggacatctc cccgatcagg cgctgcatcg cgccttcaac cgcatccaaa
     4261 tacttctcgt cgggagagag caggatcgct tgcgccgcct cgtcgtgctc ggcctgcgag
     4321 agcaggtcca tcgccaccca gtcggccggg gtgctgccgt ccgagatgac gaggacctcg
     4381 gaaggtccgg ccagcatgtc gatgccgact tcgccgaaca cctggcgttt ggcctcggcc
     4441 acgtagacgt tgcccgggcc gacgatcttg tcgactcgcg gcaccgattc ggtgccgtac
     4501 gcgagcgccg cgatcgcctg cgcgccgccg atcgcgatga cgcggtcgac gccggcgagc
     4561 gccgcggcgg cgagcaccag cgcgccgggc tgcggactga ccatcaccag ctcgcgcacg
     4621 cccgcaacct tggccggaac cgcgttcatc agcaccgtgg acggatacgc ggccttg
//
LOCUS       DASPXW010000403         2161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1126941, whole genome shotgun sequence.
ACCESSION   DASPXW010000403 DASPXW010000000
VERSION     DASPXW010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1126941"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VEL04_09235"
     CDS             <1..835
                     /locus_tag="VEL04_09235"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYT48593.1"
                     /translation="ARLAAQHLASVLGQQFVIDNKPGGGTLVGMDAVLHAPPDGHTLY
                     LSPSTTTSMHVARKSMPYDVTKVFVPVTQLVELPQALIIHPSVAAQTTREFIALAKRE
                     PGKLTYGSAGIGTAPHMAMELLKSMAGIDVQHIPYRGVSQSVTDILGGRVSGMILNVL
                     TSKPHVDQGAMRALGVTGPKRSEAMPTIPTIDESALPGYEALQWFGLLAPAGTPEPIL
                     GLLQMKLAEGFRTPEMKARLAGDGAEPVVSTPAEFAARMKSEIEKWSALAKSANIQPE
                     E"
     gene            complement(1052..>2161)
                     /locus_tag="VEL04_09240"
     CDS             complement(1052..>2161)
                     /locus_tag="VEL04_09240"
                     /inference="COORDINATES: protein motif:HMM:NF025930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48594.1"
                     /translation="YPVEFLAASMTLDAGNTDKLAEFRAEAQRLGIKVMPPSVNRSGF
                     AFEVDGDTIHYSLAALKGVGAQAVEAIVAARGDRPFTDLSDFATRINPRAVNKRVLES
                     LAAAGAFDALESNRARVFAAVDVMLGEAQRAHETKESGALEFSFGDSSTRGALRLPKV
                     EPWLPAERLQKEFDAIGFFLSGHPLDDYMPALKRMRVQSWVEFTRAVKSGVSAGRVAA
                     TVVARTERRTRTGSKMGIIGLSDPSGHYEAVLFSEGLQQYRDLLEPGSAVLLMLSAEL
                     QGEEVRARIQMVEPLDKAAAKLQKALRVFLRDQTPIESVAKRLDGQGDGEVSVVLLLG
                     QGTEVEVKLPGRFKVSPQVAGAIKAIPGVIEVQAL"
BASE COUNT          369 a          752 c          705 g          335 t
ORIGIN      
        1 cgcgcggctc gccgcgcagc atctcgcgag cgtgctcggg cagcagttcg taatcgacaa
       61 caagccgggc ggcggcacgc tggtcggcat ggacgccgtc ctgcacgcac cgcccgacgg
      121 ccatacgctc tatctctcgc cgagcacgac cacgtcgatg cacgtcgcgc gcaagtcgat
      181 gccatacgac gtgaccaagg tgttcgtgcc ggtgacgcag ctcgtcgagc tgccgcaggc
      241 gctcatcatt catccttcgg tcgcagccca gaccaccagg gagttcatcg cgctcgccaa
      301 gcgcgagccc ggcaagctca cctatggctc ggccggcatt ggaaccgcgc cgcacatggc
      361 gatggagctg ttgaagagca tggccggcat cgacgtccag cacatcccct accgcggggt
      421 gtcccagtca gtcaccgaca ttctgggcgg tcgtgtctcc ggcatgatcc tcaacgttct
      481 gacctcgaag ccgcatgtcg accagggcgc gatgcgcgcg ctcggcgtca ccggccccaa
      541 gcgcagcgag gcgatgccga cgattccgac catcgacgaa agcgcattac cgggctacga
      601 ggcgctgcag tggttcgggc tgctggcgcc ggccggcacg ccggagccga tcctcggact
      661 cctgcagatg aaactcgccg agggcttccg cacgcccgag atgaaggcgc ggctcgccgg
      721 cgacggcgcc gagccggtgg tgagcacgcc cgcggaattc gccgcccgca tgaagtccga
      781 gattgagaaa tggtcggcgc tggcgaagtc ggcgaatatc cagccggagg agtaggcgcc
      841 ttacacggac ggaacctacg cacgcaacac gtctccggcg cctactactg tgccctcccc
      901 ccgcttgcgg ggagagggca gcgacagacg ttctgcaagc acagtcgggt gaggggctgc
      961 tgcttccccc tcacccaaca gagtttgttg aaatgccttc atgccctctc cccgcgtgcg
     1021 gggagagggc gcagtaagga gcgctgcaaa attatagcgc ctgcacctcg atcacgcccg
     1081 ggatggcttt gatcgcgccg gcgacctgcg gcgagacctt gaagcgcccg ggcagcttta
     1141 cctcgacctc ggtgccctgc ccgagcagca gcaccaccga gacttcgccg tcaccctgac
     1201 catcgaggcg cttggccacg ctctcgatcg gcgtctgatc acgcaggaac acgcgcagcg
     1261 ctttctgcag cttggcagct gccttgtcga gcggctcgac catctggatg cgcgcacgca
     1321 cttcctcgcc ctgcagctcg gctgacagca tcaggagcac cgcgctgccg ggctcgagca
     1381 ggtcgcggta ctgctgcagc ccttcagaga acagcaccgc ctcgtagtgg ccggacggat
     1441 cggacagccc gatgatgccc atcttgctgc cggtgcgggt gcgccgctcg gtgcgcgcca
     1501 cgacagtggc ggcgacgcgc ccggcgctca cccccgactt cactgcccgg gtgaactcca
     1561 cccatgactg cacccgcatg cgcttgagcg ccggcatgta gtcgtcgagc ggatggccgg
     1621 agagaaagaa cccgatggca tcgaactcct tctgcagccg ctcggctggc agccacggct
     1681 cgaccttggg caggcgcaac gcgccgcgcg tggagctgtc gccgaacgag aactcgagcg
     1741 cgccgctttc cttggtctcg tgcgcgcgtt gcgcctcccc cagcatgacg tcgaccgcgg
     1801 caaacacgcg ggcgcggttg ctctccagcg cgtcgaacgc gccggcggcg gcgagacttt
     1861 ccagcacgcg cttgttcacc gcgcgcggat tgatgcgggt cgcgaaatca gagaggtcgg
     1921 tgaagggacg atcgccacgc gccgcgacga tcgcctcgac cgcctgcgcg ccgacgcctt
     1981 tgagcgcggc gagcgagtag tgaatggtgt cgccgtcgac ctcgaaggca aagcccgagc
     2041 gattgaccga gggcggcatc accttgatgc caagccgctg cgcctcggcg cggaactccg
     2101 cgagcttgtc ggtgttgccg gcatcgagcg tcatcgaggc ggcgaggaac tcgaccggat
     2161 a
//
LOCUS       DASPXW010000404         3895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1504514, whole genome shotgun sequence.
ACCESSION   DASPXW010000404 DASPXW010000000
VERSION     DASPXW010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1504514"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /gene="hisG"
                     /locus_tag="VEL04_09245"
     CDS             complement(<1..332)
                     /gene="hisG"
                     /locus_tag="VEL04_09245"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012327780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HYT48595.1"
                     /translation="MASISIALSKGRIFDETAPLLARVGIRPREDPDTSRRLVIGTQR
                     RDMRLIVVRASDTPTYVQHGGADLGIAGMDVLAEHGGEGLYQPVDLGIARCRMVVAVR
                     KGFDYAAAV"
     gene            complement(375..1628)
                     /gene="murA"
                     /locus_tag="VEL04_09250"
     CDS             complement(375..1628)
                     /gene="murA"
                     /locus_tag="VEL04_09250"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HYT48596.1"
                     /translation="MDKLRITGGRPLEGEVRVSGAKNAALPIMCAALLTEHPLALTNV
                     PRLMDVRTMAKLLGQMGVSVGAQADDKLEIQATTVSNPEAPYELVKTMRASVMVLGPL
                     LARCGRARVSLPGGCAIGSRPVDQHLKGLQAMGATISVEHGYMNARTDRLRGTRIVMD
                     LVTVTGTENLMLAATLAEGTTLLENAAREPEIVDLAKCLAAMGARIEGAGSDTIRIEG
                     VRTLSGAKHRVMPDRIETGTFLAAAAAAGGRIRLTGVAADSLDATLEKLREAGARISA
                     KQGVLEIEMTDRPASVSLRTAPYPGFATDMQAQFMALAAVARGTAMITETIFDNRFQH
                     ALELQRLGANIAIRGDTAVVKGVERLQGAKVMATDLRASAGLVIAGLVAEGETVVDRI
                     YHLDRGYEALEKKLSALGARIERVR"
     gene            complement(1621..1857)
                     /locus_tag="VEL04_09255"
     CDS             complement(1621..1857)
                     /locus_tag="VEL04_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990229 - iron-sulfur cluster assembly
                     complex [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HYT48597.1"
                     /translation="MVTAESVKAGIEAGLACEHLEVAGDGQHFQALVVSRAFAGRSRV
                     QRHQLVYAALGERMREEIHALSMRTLTPEEWQAG"
     gene            complement(1859..2614)
                     /locus_tag="VEL04_09260"
     CDS             complement(1859..2614)
                     /locus_tag="VEL04_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYT48598.1"
                     /translation="MTRFWTLFYKELLRFWKVSVQTVAAPVLSALLYLVIFGHALEGR
                     VQVYDGVRYSSFLVPGLVMMSVLQNAFANSSSSLIQSKITGNIVFVLLPPISYLEFFG
                     AYVLASVVRGLVVGAGVAAVTVWFVELRLEAPLWVLAFALLGAALLGTLGLVAGICSD
                     KIDQLAAFQNFGIIPLTFLSGVFYSIHSLPRFWQGLSHANPFFYMVDGFRYGFFGVSD
                     FPPTASLAMVAASFFAVSLLALGLLRSGYKLRQ"
     gene            complement(2611..3510)
                     /locus_tag="VEL04_09265"
     CDS             complement(2611..3510)
                     /locus_tag="VEL04_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYT48599.1"
                     /translation="MNAIEVRDVEKRYGALRALAGVSFSVEQGEFFGLLGPNGAGKTT
                     LINLIAGLARADMGSVKVMGADVVTQYRLARRMLGVVPQELVFDPFFSVRETLRLQSG
                     YFGIRANDTWIDEVMHHLDLAAKADVNMRALSGGMKRRVLVAQALVHKPPVIVLDEPT
                     AGVDVEMRQGLWQFVRRLNRDGHTIVLTTHYLEEAELHCNRIAMLKAGRIAALDSTKN
                     LLGTFSGLELRLLLDREGLPEPFPGRLIARDGAQFRLQLATYRDLEAALARLREAGIE
                     IIEIELQPPDLEEVFLRLTGRQS"
     gene            complement(3507..>3895)
                     /locus_tag="VEL04_09270"
     CDS             complement(3507..>3895)
                     /locus_tag="VEL04_09270"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HYT48600.1"
                     /translation="RVGHRRPDQEPEREKPRAREFRRGGAEMIRREGGRMMVSGSVTL
                     ANAADLLEEGRRHLEEGVRTVDFGEVTELDSALLALLLAWLRDAKSREREIAFANLPE
                     SVQTIARLYGVHDLLPLHGTAAASHP"
BASE COUNT          664 a         1349 c         1255 g          627 t
ORIGIN      
        1 acggccgccg cgtagtcaaa gcccttgcgg acggccacca ccatgcggca gcgggcgatg
       61 ccgagatcca ccggctggta aagcccttcg ccgccatgct cggcgagcac atccatgcct
      121 gcgatgccga ggtcggcacc gccgtgctgc acgtacgtgg gggtgtccga agcgcgcacc
      181 acgatcaggc gcatgtcgcg cctttgggtg cctatgacga gcctgcggga ggtgtccgga
      241 tcttccctag gccggatacc gacgcgcgcg agcaggggcg cggtctcgtc gaagatccgc
      301 cccttcgata acgcaatact tatgctcgcc atttgccata tccgggccgc attctacctc
      361 aaggggggaa actcttacct gacgcgctcg atgcgggcgc ccagggcgga gagctttttc
      421 tccagcgcct cgtagccgcg gtcgagatgg tagatgcggt cgaccacggt ctcgccctcg
      481 gcgaccagcc ccgcgatgac caggcccgca gaggcgcgca gatcggtcgc catcaccttc
      541 gcgccctgca agcgttcgac gcccttcacc accgcggtgt ctccgcgaat cgcgatgttt
      601 gcgcccagac gctgcagttc gagcgcgtgc tggaagcggt tatcgaagat ggtctcggtg
      661 atcatcgccg tgccgcgcgc caccgccgcg agcgccatga actgcgcctg catgtcggtg
      721 gcgaatccgg ggtacggcgc cgtccgcaag ctgaccgaag ccggacgatc cgtcatctcg
      781 atttccagaa caccttgttt ggcggaaatt cttgctcccg cttcgcgcaa tttttccagc
      841 gtcgcgtcga gcgagtcggc ggctaccccg gtcagcctga tccggccgcc cgccgccgca
      901 gccgcggcga gaaaggtccc ggtttcgatg cggtcgggca tcacccggtg cttggctccg
      961 gaaagggtcc gcacgccctc gatccggatc gtatcgctgc ccgcgccttc gatccgagcc
     1021 cccatcgcag cgaggcactt cgcgaggtcc acgatctccg gctcgcgcgc cgcgttctcc
     1081 agcagcgtcg tgccctccgc aagcgttgcc gccaacatca gattctccgt gccggtgacc
     1141 gttaccagat ccatcacgat ccgggtcccc cgcaggcggt cggtgcgcgc gttcatgtag
     1201 ccgtgctcga cggagattgt cgcgcccatc gcctgcaggc ccttcagatg ctggtccacg
     1261 ggtcgcgagc cgatcgcgca gccgccgggc agcgacacgc gggcgcgccc gcagcgcgca
     1321 agcagcggac cgagcaccat caccgaggcg cgcatcgtct ttaccagctc gtagggcgcc
     1381 tcgggattgc tcaccgtcgt ggcctgaatt tcgagcttgt cgtcagcctg cgcgccaacc
     1441 gagacaccca tctgcccgag cagtttcgcc atcgtgcgca cgtccatcag cctgggcaca
     1501 ttcgtcaagg cgagcggatg ctccgtaagc agcgcggcac acatgatcgg cagcgcggcg
     1561 ttctttgccc ccgagacgcg cacttcgccc tcgagcggcc gcccgccagt gatgcgcagc
     1621 ttatccagcc tgccattcct ccggggtgag cgtgcgcatc gacagcgcgt ggatttcctc
     1681 gcgcatgcgc tcgccgagcg ccgcgtacac cagctggtgg cgctgcacgc ggctgcggcc
     1741 ggcgaaggcg cgcgatacca ccagcgcctg gaagtgctgg ccgtcgcccg cgacctcgag
     1801 gtgctcgcag gcgagtcccg cctcgatccc ggccttcacg ctttccgctg tcaccacttt
     1861 actgcctcaa tttgtaacct gacctgagaa gccccagcgc cagcaacgat acggcaaaaa
     1921 aactcgccgc gaccatcgcg aggcttgccg tgggcgggaa gtcggacacg ccgaagaagc
     1981 cgtagcggaa gccgtccacc atgtagaaga acgggttggc gtgcgacagg ccctgccaga
     2041 agcgggggag cgaatggatc gaatagaaca cgcccgacag gaaagtgagc ggaatgatgc
     2101 cgaagttctg gaacgccgcc agctggtcga tcttgtccga gcagatgccg gccaccagcc
     2161 cgagcgtgcc gagcagcgcc gcgccgagca gcgcgaaggc gagcacccag agcggcgcct
     2221 cgagcctgag ctcgacgaac cacaccgtga ccgccgccac ccccgcgccc acgacgaggc
     2281 cgcgcaccac cgaagcgagc acgtaggcgc cgaagaactc gaggtacgag atcggcggca
     2341 gcagcacaaa cacgatgttg ccggtgatct tggactgaat caggctcgac gagctgttgg
     2401 cgaaggcatt ctgcagcacg ctcatcatca ccagtcccgg caccaggaaa gagctgtagc
     2461 gcacgccgtc gtagacctgc acccgtcctt cgagcgcgtg gccgaagatc accaggtaga
     2521 gaagcgcgct gagcaccggg gcggctacag tctgcaccga caccttccag aagcgcagca
     2581 gctccttgta gaacagcgtc cagaagcggg tcacgattgc cggccggtga ggcgcaggaa
     2641 tacttcttcg aggtcggggg gctggagctc gatttcgatg atttcgatgc ccgcctcccg
     2701 caggcgggca agcgccgcct cgaggtcgcg gtaggtggcg agctgcagcc tgaactgcgc
     2761 cccgtcgcgc gcgatgagcc tgccgggaaa cggctccggc agcccttcgc ggtcgagcag
     2821 cagccgcagc tccagcccgg aaaacgtgcc gagcaggttc ttggtcgagt cgagcgcggc
     2881 gatgcggcct gccttcagca tggcgatgcg gttgcaatgc agctcggctt cctccaggta
     2941 atgggtggtg agcacgatgg tgtggccgtc gcggttgagc cgccgcacga actgccacag
     3001 cccctggcgc atctcgacgt cgacgcccgc ggtcggctcg tcgagcacga tcacgggcgg
     3061 cttgtgcacc agcgcctggg cgaccagcac ccgccgcttc atgccgcccg agagcgcgcg
     3121 catgttgacg tcggccttgg cggcgagatc gaggtgatgc atcacctcgt cgatccaagt
     3181 gtcgttcgcg cgaataccga agtagccgga ctgcaggcgc agcgtctcgc gcacgctgaa
     3241 gaaaggatca aacaccagct cctggggcac cacgccgagc atgcggcgcg cgagccggta
     3301 ttgcgtgacg acgtcggcgc ccatcacctt cacgctgccc atatcggccc gcgccagccc
     3361 ggcgatcagg ttgatgaggg tggtcttgcc ggcgccgttc gggcccagca ggccgaagaa
     3421 ttccccctgt tcgacgctga agctcacccc ggcgagcgcg cgcagcgcac cgtagcgctt
     3481 ttcgacgtcc cggacttcta ttgcgttcag gggtgcgagg cggccgcggt gccgtgcaac
     3541 ggaaggaggt cgtgcacgcc gtacaggcgg gcgatggtct gaaccgattc gggcaaattg
     3601 gcgaacgcga tctcgcgctc ccggctcttc gcgtcgcgca gccacgcgag caacagggcg
     3661 agcagcgccg aatcgagctc ggtgacttcg ccgaaatcga ccgtgcgcac gccttcctcg
     3721 agatggcgcc tgccctcctc caggaggtcc gctgcattgg cgagagtgac tgagccgctc
     3781 accatcatgc ggccgccttc gcgccggatc atttccgctc cgccgcgccg gaactctcga
     3841 gcgcgcggtt tttcgcgctc aggttcttga tcaggccgtc gatgcccgac tcgcg
//
LOCUS       DASPXW010000405         2745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1630396, whole genome shotgun sequence.
ACCESSION   DASPXW010000405 DASPXW010000000
VERSION     DASPXW010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1630396"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VEL04_09275"
     CDS             <1..250
                     /locus_tag="VEL04_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HYT48601.1"
                     /translation="EMNWCCGGGAGVFLLNRAAKLRQRTFELKMRQIDATGAEAVVMS
                     CGSCRLNFMNGQANSKWPVKIESLVELVGENLADGASS"
     gene            253..1134
                     /locus_tag="VEL04_09280"
     CDS             253..1134
                     /locus_tag="VEL04_09280"
                     /inference="COORDINATES: protein motif:HMM:NF024628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HYT48602.1"
                     /translation="MCELFAMSSRLPADTRLSLEAFARHGGLEGPHKDGWGIAYYADE
                     DVRLVKEPEPASSSACVRFIQDHPFAAAIVVGHIRRATQGRLAMKNCQPFARELGGRM
                     HVFAHNGDLDSGALRAALPLGTFRPVGDTDSEYAFCALLERLRAPWLGGELPALEERV
                     RIVAGFAASIRPLGPANFLYSDSDALFIHGHKRFHGDGAGARPPGLHVLCRRCAQQGT
                     EQDVVIAASVPLTKEPGWRALAEGELVVAQRGGAVAAVYPAATACCTTSPAASSTLTA
                     TGMPRRMLSVTTQKSSN"
     gene            complement(1020..1493)
                     /locus_tag="VEL04_09285"
     CDS             complement(1020..1493)
                     /locus_tag="VEL04_09285"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HYT48603.1"
                     /translation="MADAQSFTITLEQQAGFEFRVRFDWPGVAELALDEPEPLGARRG
                     PNAARLVAAAVANCLAASLVFCLKTKFKQDLGKVRASATGRLERNQRGRYRIAAIDVT
                     ITLAESSATLPHLERCMDQFEDFCVVTESIRRGIPVAVRVLDAAGEVVQHAVAAG"
     gene            1616..2509
                     /locus_tag="VEL04_09290"
     CDS             1616..2509
                     /locus_tag="VEL04_09290"
                     /inference="COORDINATES: protein motif:HMM:NF014074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48604.1"
                     /translation="MRLWSQHVANLARAAFWLLLIVIVLQQMFGSRGIDRERASLLGQ
                     FEQERGSRVIAMIHRQESASVLGVPVTGSISIDDSEAVLRAIRLTPPGQPIDVILHTP
                     GGLVLAAEQIAKALVERKGKVTVFVPHYAMSGGTLIALAADEIVMDANAVLGPVDPQI
                     GDLPAASIIKVVEFKGPAKVSDELLIMADIAQKARIQVASFVAQVLAKHMNEKQSAQI
                     ATILSEGRWTHDFPITVQAARQLGLKVSTDMPRRVYDLMDLYPQGGGIKPSVWYVPIR
                     RGAPAQPSPGPRPEGKGASNP"
     gene            complement(2506..>2745)
                     /locus_tag="VEL04_09295"
     CDS             complement(2506..>2745)
                     /locus_tag="VEL04_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HYT48605.1"
                     /translation="FGFWDVKRTREAHPRGHFNRMIEQKVSELGGIKSLYSDCFFTPD
                     EFARRYGGEFYGELKNKYDPGGAFPDLYSKCVLRR"
BASE COUNT          457 a          934 c          909 g          445 t
ORIGIN      
        1 cgagatgaac tggtgctgcg gcggcggcgc gggcgtgttc ctcctgaacc gcgcagcgaa
       61 actccggcag cgcaccttcg aactcaagat gcggcagatc gacgccaccg gcgccgaagc
      121 cgtcgtcatg tcctgcggca gctgccgcct caacttcatg aatggccagg ccaacagcaa
      181 atggccggtg aagatcgaga gcctcgttga actggtcggc gaaaacctgg ccgacggagc
      241 gagctcatag ccatgtgcga gctgtttgca atgtcgagcc gtcttcccgc ggacacgcgc
      301 ctctctctgg aggcctttgc gcgccacggc gggctcgagg ggccgcacaa ggacggctgg
      361 ggcatcgcct attacgcgga cgaagacgtg cgcctcgtca aggagccgga gcctgcttcc
      421 agcagtgcct gcgtgcgctt catccaggac catcctttcg ccgccgcgat cgtcgtcggc
      481 cacatccgcc gggcgaccca gggcaggctc gcgatgaaga actgccagcc gtttgcgcgc
      541 gagctgggcg gccgcatgca tgtcttcgcc cacaacgggg atctcgactc gggcgcgctg
      601 cgcgcggcgc tgccgctcgg cacgtttcgc ccggtgggcg acaccgactc cgaatatgct
      661 ttctgcgcgc tgctcgagcg cctgcgcgcg ccgtggctgg gcggcgagct gcccgcgctc
      721 gaagagcggg tgcgcatcgt cgcggggttc gcggcgtcga tccgcccgct cggcccggcg
      781 aacttccttt actccgacag cgacgcgctg ttcatccacg gtcacaagcg gttccacggc
      841 gacggcgccg gcgcgaggcc tcccggcctg catgtgcttt gccggcgctg cgcgcagcag
      901 ggcaccgaac aggacgtggt tattgcggcg agcgtaccgc tcaccaaaga acccggctgg
      961 cgggcgctcg ccgaaggcga gctggtggtc gcgcagcgcg gcggcgcggt cgcggcggtt
     1021 tacccggcgg ctaccgcatg ctgcaccacc tcgcccgcgg cgtcgagcac cctgacggcg
     1081 accggaatgc cgcgccggat gctttccgtg accacgcaga agtcctcgaa ctgatccatg
     1141 cagcgctcaa ggtggggcag cgtcgcggag ctctcggcca gcgtaatcgt gacgtcgatc
     1201 gcagcgatgc gatagcggcc gcgctggttg cgctcgaggc gcccggtcgc gctcgcgcgc
     1261 actttgccga gatcctgctt gaatttcgtt ttcaaacaga ataccaggct ggcggcgagg
     1321 cagttcgcca ccgccgcggc caccaggcgc gcggcattcg gcccgcgccg tgcgccgagc
     1381 ggctcgggct cgtcgagcgc gagctccgcg accccgggcc aatcgaagcg cacgcggaac
     1441 tcgaagcccg cctgctgctc gagggtgatg gtgaagctct gtgcgtcggc cattgccgca
     1501 cactaaggga taccgggcac ggcgcatttg acccacgtca aacgccgccc cgcgacaaaa
     1561 tgcaccgctc agagtaggct gctgccggat tctcaaggct gaggatggaa cccccgtgcg
     1621 cctttggtcg cagcacgtcg cgaatctcgc gcgcgccgct ttctggctgc tgctgatcgt
     1681 catcgtattg cagcagatgt tcggctcgcg tggcatcgac agggagcgcg ccagcctcct
     1741 cggccagttc gagcaggaac gcggctcgcg ggtgatcgcg atgatccatc gtcaggaaag
     1801 tgcaagcgtg ctgggcgttc cggtcacggg aagcatcagc atcgacgatt ccgaggcggt
     1861 gctgcgcgcc atccgcctca cgccgcccgg gcagccgatc gacgtcatcc tccatacgcc
     1921 gggcgggctg gtgctcgcgg ccgagcagat cgccaaggcg ctggtcgagc gcaaaggcaa
     1981 ggtgaccgtc ttcgtgccgc attacgcaat gagcggtgga acgctcattg cgttggcggc
     2041 agacgaaatc gtcatggacg ccaacgcagt gctcggccct gtcgacccgc agatcggcga
     2101 cctgcccgcg gcctccatca tcaaggtggt cgagttcaag gggcccgcca aggtaagcga
     2161 cgagctcctc atcatggccg acatcgcgca gaaggcccgc atccaggtgg cgagcttcgt
     2221 cgcgcaagtg ctggccaagc acatgaacga gaagcagtcg gcgcagatcg cgaccatcct
     2281 gtccgaaggc cgctggacgc acgattttcc gattaccgtc caggccgcgc gccagctcgg
     2341 gctcaaggtg tcgaccgaca tgccgcgccg cgtctacgac ctgatggatc tctacccgca
     2401 aggcggcggc atcaagccct cggtctggta cgtgccgatt cgccgggggg cgccggcgca
     2461 gccttcaccc gggccccgtc ccgagggtaa aggcgcatcc aacccttaac ggcgcaacac
     2521 gcactttgag tagaggtccg ggaaagcgcc gcccgggtcg tacttgttct tgagctctcc
     2581 gtaaaactcg cccccgtagc ggcgcgcgaa ttcgtccggc gtgaaaaagc aatccgaata
     2641 gagcgacttg atgccgccca gctcgctcac tttttgctcg atcatgcggt tgaagtggcc
     2701 gcgcggatgc gcctcgcgcg tgcgcttcac gtcccagaag ccgaa
//
LOCUS       DASPXW010000406         3907 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_494627, whole genome shotgun sequence.
ACCESSION   DASPXW010000406 DASPXW010000000
VERSION     DASPXW010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494627"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..1296)
                     /locus_tag="VEL04_09300"
     CDS             complement(130..1296)
                     /locus_tag="VEL04_09300"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HYT48606.1"
                     /translation="MTRFAPLLLTVFFLPALALAVPATPEDRLSAVFQALEANRLDEA
                     LGRVDALIRDYPNFRLAHLVRGDLLLARARPLQTFGNVVKTVPQERIEGLREEALARL
                     RALRERPAEDRLPRYVLQLHSGQKHVLVVDSRRSRLYVFANDAGRARLVADYYVTLGK
                     NGMEKTREGDQKTPVGVYHVTANLPRSKLTDFYGAGAFPINYPNEWDRRLGRKGHGIW
                     LHGVPSDVYSRPPRASDGCIVLANPDLESVGRAVQVGTTPVIIADAIEWSDAAAIEAE
                     RSSLGEALERWRADWQSRDTERYLEHYSQRFSSGSHDYRAWAAYKRSATSAKRWIEVG
                     LADVAMFRHPRELDFVVVSFTQAYRSDRLSNTIRKRQYWIREDGAWKILYEGAA"
     gene            complement(1293..2258)
                     /locus_tag="VEL04_09305"
     CDS             complement(1293..2258)
                     /locus_tag="VEL04_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYT48607.1"
                     /translation="MRISSAPRKYLLRGLLALCLAAFYAATPARADDLQDASQLLKAG
                     QREQALERVNRALAAKPADPQARFLKGLIYTEQGNAREAIEIFTKLTQDYPDLPEPYN
                     NLAVIYAGQGQYEKARAALEQSIRTHPSYATAYENLGDVYAKLASQAYDKALQLDSSN
                     TGAQNKLSLVRELVGGPATVVAKEAPAKPSVVAEKPSAPVEKPQPASNPTAEILKTVN
                     GWAQAWSRKDAEAYLAYYARDFKVPGGEPREAWEKSRRDRIAAPKSIAVGVDSPKVTL
                     LGSDQASVTFRQSYRSDKLKSSSRKTLVLSKAGGRWQIREEKSAK"
     gene            2298..3653
                     /gene="cysS"
                     /locus_tag="VEL04_09310"
     CDS             2298..3653
                     /gene="cysS"
                     /locus_tag="VEL04_09310"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011614939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HYT48608.1"
                     /translation="MLKIYNSLGRAKQEFVPLNPPEVRMYVCGMTVYDFMHLGHARML
                     VAFDVVARYLRALGYRLTYVRNITDIDDKIIKRANEKGEPIQALTERFIRANEEDYAA
                     LGLLRPEHEPRATQSIAGMIRMIGELIARGHAYAGANGDVYYDVSSFEGYGRLSGKEP
                     QALRAGARVEVDEAKHDPLDFVLWKAAKPGEPAWDSPWGRGRPGWHIECSVMSTELLG
                     SRFDIHGGGEDLQFPHHENEIAQSEGANGVKFVNYWMHNGFVRVDEEKMSKSLGNFFT
                     VREVLKKYDAEVVRFFILRAHYRSPLNYSDAHLEDAKSALTRLYTALKGSSREFFSVD
                     WNEPHAGRFKEAMDDDFATPEAVAVLFDLAHEVNSGNTIAATRLRALGGVLGILQRGA
                     EEFLQGGGSGDDWITERITAREAARKRKDYAAADDIRRELAEKGIVLEDKGGRTTWRR
                     K"
     gene            3650..>3907
                     /locus_tag="VEL04_09315"
     CDS             3650..>3907
                     /locus_tag="VEL04_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HYT48609.1"
                     /translation="MTATDSTLVHPLVVRITHWINVLAVLIMITSGWRIYNASPLFES
                     FSFPDELTLGGWLAGALQWHFAAMWLFALNGLVYVVYGIASG"
BASE COUNT          669 a         1243 c         1304 g          691 t
ORIGIN      
        1 gcgctttcgc cgggtagagt tcgaggcgga tggtgccggc gctggtgcgc acgtcgacct
       61 gggggtcggc ggcgagcgcg cccgccggga cgaccgctgc cgcggaaagc acgaacaaat
      121 gcctgagttt catgccgctc cttcgtaaag aattttccag gcgccgtctt ccctgatcca
      181 gtactggcgc ttgcgtatgg tgttcgacag ccggtccgag cggtaagcct gcgtgaagct
      241 caccaccacg aagtcgagct cgcgcgggtg gcggaacatc gccacgtccg cgaggccgac
      301 ttcgatccag cgcttggcgg aagtggcgct gcgcttgtac gccgcccagg cgcggtagtc
      361 gtggctgcca gaggagaagc gctgcgagta atgctcgagg tagcgctcgg tgtcgcggct
      421 ctgccagtcg gcgcgccagc gctcgagcgc ctcgccaagc gagctgcgct cggcctcgat
      481 cgccgcggca tcgctccatt cgatcgcatc ggcaatgatc accggcgtcg tgccgacctg
      541 caccgcgcgc cccaccgact cgaggtccgg gttggcgagc acgatgcagc cgtcgctcgc
      601 gcgcggcggc cggctgtaga cgtccgaggg cacgccgtgc agccagatgc catggccctt
      661 gcggccgagc ctgcggtccc actcgttcgg atagttgatc ggaaacgcgc cggcgccgta
      721 gaaatcggtc agcttggaac gcggcaggtt ggcggtgacg tggtagaccc cgacaggggt
      781 cttttggtcg ccctcgcgcg tcttctccat gccgttcttg ccgagcgtca cgtagtagtc
      841 ggccaccagc cgcgcccggc ctgcatcgtt ggcgaacaca tacagccgcg agcgccgcga
      901 atcgaccacc agcacgtgct tctgcccgga atgcagctgc agcacgtagc gcggcaggcg
      961 gtcctccgcc ggccgctcgc gcagcgcgcg caggcgcgca agcgcctcct cgcgcagtcc
     1021 ttcgatcctc tcctggggga cggtcttcac gacattgccg aaggtctgta gcggccgcgc
     1081 gcgggcgagc agcaggtccc cgcgcaccag gtgcgcgagg cggaaattgg gatagtcgcg
     1141 gatcagcgcg tcgacgcgcc cgagcgcctc gtcaagacgg ttggcctcga gcgcctggaa
     1201 caccgccgag agccggtcct cgggcgttgc gggaacggca agcgccaggg cggggagaaa
     1261 aaacaccgtc agaaggagag gtgcgaatct cgtcatttag ccgatttctc ctcgcggatc
     1321 tgccagcgac ccccggcctt ggagagaacc agcgtcttgc ggctcgagct cttcagcttg
     1381 tcggaacggt aactctgccg gaacgtgacg ctcgcctggt cgcttccgag cagggtcacc
     1441 ttgggcgagt cgaccccgac cgcgatcgat ttcggcgcgg cgatgcgatc gcgccgcgac
     1501 ttctcccatg cctcgcgcgg ctcgccgccg gggaccttga agtccctggc gtaataggcg
     1561 aggtaggcct cggcgtcctt cctggaccag gcttgcgccc agccgtttac cgtcttgagg
     1621 atctccgccg tcgggtttga ggcaggctgc ggcttttcca cgggcgcaga gggtttttcg
     1681 gcgacgacag acggtttggc gggagcctcc tttgccacga ccgtggcagg gcccccaacc
     1741 agctcgcgca ccagcgaaag cttgttctgg gcaccggtat tcgacgaatc gagctgcagc
     1801 gccttgtcgt acgcctggct cgcgagcttg gcgtaaacgt cgcccaggtt ctcgtatgcc
     1861 gtggcatagc ttggatgggt gcggatcgat tgctcgaggg cggcgcgcgc cttctcgtac
     1921 tggccctggc cggcgtagat caccgcgagg ttgttgtagg gctcaggcag atcgggatag
     1981 tcctgcgtca gcttggtgaa gatctcgatg gcttcccgcg cattgccctg ctcggtgtag
     2041 atcagcccct tcaggaagcg cgcctgcggg tcagccggct tcgcggcaag ggcgcgattg
     2101 acgcgctcga gcgcttgctc gcgctgccct gccttgagca gctgcgaggc gtcctggagg
     2161 tcgtcggcgc gcgccggcgt cgcggcgtaa aacgctgcca ggcaaagcgc gagcaaaccc
     2221 cgcagcaagt atttgcgcgg ggcgctggaa atccgcattg tgttatattt tcaaggccct
     2281 acgaaaggtc gcattctatc ctaaagatct ataacagcct aggcagggcg aagcaggagt
     2341 tcgtacctct caatccgcca gaagtgcgca tgtacgtctg cggcatgacc gtgtacgact
     2401 tcatgcacct cggtcacgcc aggatgctgg tggcgttcga tgtcgtagcc cgctacctgc
     2461 gggcgctcgg ttaccggctg acctacgtgc gcaacatcac ggacatcgac gacaagatca
     2521 tcaaacgcgc caacgagaaa ggcgagccca tccaggcgct caccgagcgt ttcattcgtg
     2581 ccaacgagga agactatgcg gcgctcgggc tgctcaggcc cgagcacgaa ccacgcgcca
     2641 cgcagtcgat cgccggcatg atccgcatga ttggcgagct catcgcgcgc ggtcatgcct
     2701 acgccggcgc caacggcgac gtgtactacg acgtctcctc tttcgagggc tatgggcgcc
     2761 tttcgggcaa agagccccag gctctgcgcg cgggggcgcg cgtcgaggtg gacgaagcga
     2821 agcacgatcc gctggatttc gtgctctgga aagcggccaa acccggcgag ccggcttggg
     2881 actcgccttg gggccggggc cggcccggtt ggcacatcga gtgctccgtg atgtcgacgg
     2941 aactgctcgg cagccgcttc gacatccatg ggggcggcga agacctgcag ttcccgcacc
     3001 acgagaacga gatcgcgcag tcggaaggcg ccaacggcgt caagttcgtg aactactgga
     3061 tgcacaacgg cttcgtgcgc gtcgacgagg agaagatgtc gaagtcgctc ggcaatttct
     3121 tcaccgtgcg cgaggtgctg aagaaatacg acgccgaagt ggtccgcttc ttcatcctgc
     3181 gcgcccacta ccgcagcccg ctcaactatt ccgacgcgca cctggaggac gcgaagtccg
     3241 ccctcaccag gctttatacc gccctcaagg gttcttcaag agaattcttt tcggtggact
     3301 ggaacgaacc gcacgccggt cgcttcaaag aggcgatgga cgacgatttc gctacgcccg
     3361 aagccgttgc ggtgcttttc gatcttgctc atgaagtgaa tagcggaaat acaattgccg
     3421 caacccggct gcgcgcgctc ggcggcgtgc tggggatcct gcagcgcggt gcagaggagt
     3481 tcctgcaggg cggcggcagc ggggacgact ggatcacgga gcgcatcaca gcgcgagagg
     3541 cggcacgcaa gcgtaaggat tacgccgcgg cggacgacat acgccgggag ctcgccgaaa
     3601 agggtatcgt gctcgaagac aagggagggc ggaccacgtg gcgcaggaag tgaccgcgac
     3661 agacagtacg ctggtgcatc cgctggtggt gcgcatcacg cactggatca atgtccttgc
     3721 cgtgctaatc atgatcacga gcggctggcg gatctacaac gcttcgccgc tcttcgagtc
     3781 attcagcttc cccgacgagc tgaccctcgg cggctggctc gccggggcgc tgcaatggca
     3841 tttcgcagcg atgtggctgt tcgccctgaa cgggctggtg tacgtggtct acggcatcgc
     3901 ctcgggg
//
LOCUS       DASPXW010000407         2302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_749028, whole genome shotgun sequence.
ACCESSION   DASPXW010000407 DASPXW010000000
VERSION     DASPXW010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749028"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="VEL04_09320"
     CDS             complement(<1..835)
                     /locus_tag="VEL04_09320"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HYT48610.1"
                     /translation="MTDLAQAINIEDLRVMARRRVPRAVFDFFDGGAEDEVTLRENRA
                     AFERVRLLPKVLVNVAEVDLRTRIFGVESRLPMAIAPTGGISAGRAGAELALARAAKA
                     AGVPFTLATPAAFTIERVAEEAGGRLWFQLYAVRDREFREKLLLRAKNSDYEAVLVTV
                     DLPVSGKRERDPRNGFVTPYKPNWRNSRDVLFKPAWLLEILRHGLPGMANFEGYRFST
                     PEGTDIVTAVGREMDASLDWEELKRLRDLWPRKLLLKGVERRDDAERAAALGCDGVVV
                     SN"
     gene            886..1809
                     /locus_tag="VEL04_09325"
     CDS             886..1809
                     /locus_tag="VEL04_09325"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HYT48611.1"
                     /translation="MAKVKRIEGVLAPVITPFKRDYAPDADRFVRHCRWLLKSGCAGL
                     AVFGTNSEANSMSVSEKRTLLEALVAGGVSPATLMPGTGHCALSDSIELTRAAVELGC
                     AGVLMLPPFYYKGVPDEGLYRNFAEVIERVGDERLQLYLYHIPPVSQVGISLNLIEKL
                     ISKYPGIVAGVKDSSGDWSNTKAMLDAFAKEGFDVFAGSEVFLLDNMRNGGKGCITAT
                     GNVNPGAIDRLYRNWRSADADRMQAAITATRKIVQKQPMIPALKAVVAHFGNDPRWST
                     CRPPLIELNASQEKELISELKANGFAMPGLA"
     gene            1806..>2302
                     /locus_tag="VEL04_09330"
     CDS             1806..>2302
                     /locus_tag="VEL04_09330"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYT48612.1"
                     /translation="MKTALVTGAARGIGRAAAEALEKSGFRVLRLDVQGEISYDLTNL
                     AGIPKLVDSLGEIHVLVNNAGVQNAVGIDRYTDAQRERILRINLEAPVELIRSVSKQM
                     VARGSGRIINLASVAAYTAHTDLWYGVTKAGVVSYTRSFASYLGPHGIQVNAVAPGPI
                     DTELL"
BASE COUNT          402 a          797 c          737 g          366 t
ORIGIN      
        1 ggttcgacac caccacgccg tcgcagccga gcgcggccgc gcgctcggcg tcgtcgcgcc
       61 gttcgacgcc cttcagcagc agcttgcgcg gccagagatc gcgcaggcgc ttcaattcct
      121 cccagtcgag agaggcatcc atctcgcgcc cgaccgcggt gacgatgtcg gtgccctcgg
      181 gcgtgctgaa gcgatagccc tcgaagttcg ccatgcccgg caggccgtgg cgcaggattt
      241 caagcagcca cgccggcttg aagagcacgt cgcgcgagtt gcgccagttc ggcttgtagg
      301 gcgtgacgaa accgttcctg ggatcgcgct cgcgctttcc ggacaccggc agatcgaccg
      361 tgacgagcac cgcctcgtaa tcggaatttt ttgctctgag aagcaatttt tcccggaact
      421 cgcgatcgcg caccgcgtag agctggaacc agagccgccc gcccgcctcc tcggcaacgc
      481 gctcgatggt gaaggcggcg ggggtggcga gcgtgaacgg cacgccggca gccttggcgg
      541 cgcgcgcgag cgcgagctcc gccccggcgc ggccggcgct aatgccgccg gtgggggcga
      601 tcgccatcgg cagcctggac tcgacgccga aaatccgcgt ccgcaggtcc acctcggcca
      661 cgttcaccag caccttcggc agcagccgca cgcgctcgaa cgcggcgcgg ttctcgcgca
      721 gcgtcacctc gtcctcggcg ccgccgtcga agaaatcgaa caccgcgcgc ggcacgcggc
      781 ggcgcgccat cacgcgcagg tcctcgatgt tgatggcttg agcgaggtcg gtcatataat
      841 tcgcgctcgc cactctaccg gaaaggcagc gccgtgaaac cagccatggc caaggtgaag
      901 cgcatcgagg gcgtgctcgc cccggtcatc acgcccttca agcgcgacta cgcgcccgac
      961 gccgatcggt tcgtgcgcca ctgccgctgg ctgctgaaga gcggctgcgc cgggctggcg
     1021 gtgttcggca ccaacagcga ggcgaactcg atgtccgtct ccgaaaaaag aacattgctg
     1081 gaggcgctgg tggccggcgg cgtatcgccg gcgacgctga tgcccggcac cgggcattgc
     1141 gcgctcagcg attcgatcga gctgacgcgc gccgcggtcg agctcggctg cgccggcgtg
     1201 ctgatgctgc cgccgttcta ttacaagggg gtgccggacg aaggcctgta ccgcaacttc
     1261 gccgaggtga tcgagcgcgt cggcgacgag cggctgcagc tctacctcta tcacatcccg
     1321 ccggtctctc aagttggaat ttctttgaat ttgatagaga agctgatttc caagtacccg
     1381 ggaatcgtcg ccggcgtcaa ggactcctcg ggcgactggt cgaacaccaa ggcgatgctc
     1441 gacgccttcg ccaaggaagg cttcgacgtg ttcgccggca gcgaagtgtt cctgctcgac
     1501 aacatgcgca acggcggcaa gggctgcatc accgctaccg gcaacgtcaa ccccggcgcg
     1561 atcgaccgcc tctaccgcaa ctggcgctcg gccgacgccg accgcatgca ggcggcgatc
     1621 acggcgacgc gcaagatcgt gcagaagcag ccgatgatcc cggcgctgaa ggcggtcgtg
     1681 gcgcacttcg gcaacgatcc gcggtggagc acctgccgcc cgccgctgat cgagctcaat
     1741 gcttcccagg aaaaggaatt gattagcgag ctcaaggcga acggcttcgc catgcccggc
     1801 ctcgcttgaa gaccgcgctc gtcaccggcg cggcccgcgg catcggtcgc gccgctgccg
     1861 aagcgctgga aaaatcgggt tttcgtgtcc tcagactcga cgttcaaggc gaaatctcct
     1921 acgacctgac caacctggcg ggcattccga agctcgtcga ttcgctcggc gagatccacg
     1981 tcctcgtcaa caacgccggc gtgcagaacg cggtgggcat cgaccggtac accgatgcgc
     2041 agcgcgagcg cattttgcgc atcaacctcg aagccccggt cgagctgatc cgcagcgtct
     2101 cgaagcagat ggtcgcccgc ggcagcggcc gcatcatcaa cctcgcttcg gtcgcggcct
     2161 acaccgcgca taccgacctc tggtacggcg tcaccaaggc cggcgtggtc agctatacgc
     2221 gcagcttcgc ctcctacctc ggtccgcacg gcatccaggt gaatgccgtc gcgccgggac
     2281 cgatcgacac cgagctcctc aa
//
LOCUS       DASPXW010000408         4838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_494858, whole genome shotgun sequence.
ACCESSION   DASPXW010000408 DASPXW010000000
VERSION     DASPXW010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4838
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494858"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VEL04_09335"
     CDS             <1..374
                     /locus_tag="VEL04_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYT48613.1"
                     /translation="AISAPFGPHSDAQWRFLTQTWVRKSADGTWRPHYDPRIAEPFQA
                     TMREGDLVLWHVYDAIRCPTLVLRGAQSDLLSRQTVAEMARRGPKAKVVEIANVGHAP
                     TLLHADQIAMVRDFLLEGATS"
     gene            371..1366
                     /locus_tag="VEL04_09340"
     CDS             371..1366
                     /locus_tag="VEL04_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HYT48614.1"
                     /translation="MKTFLFLLLFLAGSAAAQSTLDSVRSRGFVQCGVNTGLAGFSQP
                     DSKGVWRGIDVDVCRAVAAAVFGDANKVRYTPLTAQQRFTALQSGEVDVLSRNTTWTL
                     SRDTSLGLNFVGVNYYDGQGFMVPKKLNVKSAKQLNGATICVQPGTTTELNLADYFRA
                     NRMSFKPVVIEKLEEVLNAYFAGRCDAFTTDHSGLVALRASRATKPGDHVILPEIISK
                     EPLGPAVRHGDDRWFDVVKWSLFAMLEAEELGLSSKNIDGEAKSTNPSIQRFVGASGE
                     MGKMLGVDNRWAFQIVKQVGNYAESFDANLGPLGFERGLNRLWNQGGLMYAPPIR"
     gene            1412..2167
                     /locus_tag="VEL04_09345"
     CDS             1412..2167
                     /locus_tag="VEL04_09345"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HYT48615.1"
                     /translation="MQKIVAPLFLPLPVCVLLLAVGIGLLWFSGRQRAGKVVVTAGFA
                     LLALMSYGWVSRPLLRSLEHYHVPLAAVAAGSAMKWIVVLGGTTSSDASVPPSARLSE
                     ATLARLVEGVRLHRQLPGSRLVLSGARVFGSASDAESMSALALALGVARADIVVDAAS
                     QDTETQARNIRQIIKGEPCVLVTSAAHMRRSMALFRKAGLDPIPAPTHYLAQVNQGIS
                     LAGFYPSVDGIKSAQIAVYEYLGITWAALRGKI"
     gene            complement(2164..3117)
                     /locus_tag="VEL04_09350"
     CDS             complement(2164..3117)
                     /locus_tag="VEL04_09350"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYT48616.1"
                     /translation="MKRIFLGVITALFCAGGLAQPYPSKPIRIVVAFPPGGPVDIIAR
                     LVAPKLQADLGQPVVVENRAGAGGNLPTSQAARAPADGYTLLATSSAYAVNPSLTPNP
                     GYDPQKDFVPVAIVAWQPNLIVVQADFPAKTLAELLQMARSGKLAYASPSSGTTPHLT
                     AENLFKVRAKVDITHVPFKGAGPAVAAVLGGQPPIGSMAASGPMANLRAGKLRALAVS
                     SSRRLASLPDVPTLGELGYADMEDYTWVGLFVPAATPRDIAQRLNDAVMRAVQSPEVR
                     ERFEALAFEPTAAPLRETADYVRSELVKWGRVVRDTGAKLD"
     gene            complement(3126..4805)
                     /locus_tag="VEL04_09355"
     CDS             complement(3126..4805)
                     /locus_tag="VEL04_09355"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HYT48617.1"
                     /translation="MAKLSGAQVLADMLSGYGVSHVFMVPAVLRRSFAEMERRTSIAR
                     IHTHGEKAAAYMADGYARACGRPGVCMAQVVGALNLAAGLRDAWLAHSPVLAFTGGRD
                     PKTKFRKVYQEADDVPAFEPVTKWNASVDAVERFPDMLRQAFRVATSGAPGPVHLQFR
                     GNEGQIDQEEAEMEPQVEPQFARAPAFRPVPDSQSVKTAVDILQKAERPVLVAGGGAR
                     WSGAGPELAALAEALCIPVVTSLNGKDCIAASHPLCAGVVGSYSRESANRVVAAADLV
                     CFVGTSAGGMTTHVWSVPKIGVAAIQIDIEPEVIGRNYPLRAGVLGDAKTTLAEMLKL
                     ADRSTASRRTSWISQVKGICEQWRDKYRAALESDAVPIRPERICAELSRHVPEDAIVL
                     SDTGHAGMWMGGMYDVRSPRQSFLRSAGHLGWAFPAGLGAKCAAPQRPVVTFTGDAGL
                     WYHLGEIETAVRWNIGAVTVVNNNASGNQSKRGFDRAYGGEQTAKARELWTYRMVDFA
                     RIAADIGAFGIRVERAADFPAVLQKALDSGKPAIIDVVTDIEAIAPAAVSG"
BASE COUNT          786 a         1601 c         1611 g          840 t
ORIGIN      
        1 gcgccatctc ggcgcccttc ggtccgcaca gcgacgcgca atggcgcttc ctgacgcaga
       61 cctgggtgcg caagagcgcc gatggcacct ggcggccgca ttacgacccg cgcatcgccg
      121 agccgttcca ggccacgatg cgcgagggcg acctggtgct atggcacgtc tacgacgcaa
      181 tacgctgccc gaccctggtg ctgcgtggcg cgcagtcgga cttgctcagc cggcagacgg
      241 ttgcagaaat ggcgcgccgc gggccgaaag cgaaagtggt cgagatcgcc aacgtgggcc
      301 acgccccgac tctgctgcat gccgaccaga tcgcgatggt ccgggatttc ctgctcgaag
      361 gagcgacctc atgaagactt ttctgtttct actgttattc ctggcgggaa gtgcggctgc
      421 tcaatcgacg ctcgattcgg tccgctccag aggtttcgtc cagtgcggcg tgaataccgg
      481 acttgcgggc ttctcgcagc ccgattccaa gggcgtgtgg cgcggcatcg acgtcgatgt
      541 gtgtcgcgcg gtcgccgccg cggtgttcgg cgacgcgaac aaggtgcgct atacgccgct
      601 taccgcgcag cagcgcttca ccgccttgca gtcgggcgag gtcgacgtcc tttcgcgcaa
      661 taccacatgg accctctcga gggacaccag tctcggcttg aacttcgtag gcgtgaacta
      721 ctacgacggc cagggcttca tggtgccgaa gaagctcaac gtgaagagcg cgaagcagct
      781 gaacggcgcc actatctgcg tgcagcccgg tacgaccacc gagctcaacc tcgccgatta
      841 cttccgcgcc aacaggatga gcttcaagcc ggtggtgatc gagaagctcg aggaggtgct
      901 gaacgcttac ttcgccgggc gctgcgacgc gttcaccacc gaccactcgg gcctcgtcgc
      961 gctgcgcgcc tcgcgcgcga ccaagcccgg ggaccacgtc atcctgcccg agatcatctc
     1021 gaaagagccg ctcgggcccg cggtgcgcca tggcgacgac cgctggttcg acgtagtgaa
     1081 gtggtcgctg ttcgccatgc tcgaggccga ggaacttgga ctttcatcga aaaatatcga
     1141 tggcgaggcg aaaagtacca atccgtcgat ccagcgcttc gtcggcgcaa gcggcgagat
     1201 gggcaagatg ctcggcgtgg acaaccgctg ggcgttccag atcgtgaagc aggtcggcaa
     1261 ctacgccgag agcttcgacg ccaacctggg gccgctcggc ttcgagcgcg gcctcaaccg
     1321 cctgtggaac cagggcggtc tcatgtacgc gccgccgatc cgctagcggc cgcgccgttt
     1381 cgatttcgct ccggaggaac gcatgtttct cttgcagaag atcgtcgcgc cgttgttcct
     1441 tcccttgcca gtttgtgtgc tgctgctggc cgtcggcatc ggcctgctct ggttcagcgg
     1501 ccgccagagg gcgggcaagg tagtggtgac cgcaggcttc gctctgctgg ccctgatgag
     1561 ctacggctgg gtgtcgcgcc cgctcctgcg gtctctcgag cactatcacg tgccgttagc
     1621 cgcggtggct gccgggtcgg cgatgaaatg gatcgtcgtg ctcggcggca ctacgtcgtc
     1681 ggacgcgtca gtcccgccca gcgcgcgtct ttcggaggct accctggcga ggctggtgga
     1741 gggcgtgcgc ctgcaccggc agttgcccgg ttcccggctg gtgctgtccg gagcgcgggt
     1801 cttcggctcg gcctccgatg ccgaatccat gtccgcattg gcgctggcgc tcggggtggc
     1861 acgcgcggac atcgtggtcg acgccgcgtc ccaggacacc gagacccagg cgcgcaatat
     1921 ccggcagatc atcaagggcg aaccctgcgt cctggtcacc tcggcggcgc acatgcgccg
     1981 ctccatggcg ctcttcagga aagccggcct cgacccgatc ccggccccca cgcattacct
     2041 cgcgcaggtg aaccagggaa tcagcctggc aggcttctac ccgagcgtcg acgggatcaa
     2101 aagcgcgcag atcgctgtct acgaatactt gggaatcacc tgggccgcgc ttaggggaaa
     2161 gatctagtcg agcttcgcgc cggtgtcgcg caccaccctg ccccatttga ccagctcgga
     2221 acgcacgtaa tcggccgtct cgcgcagcgg agcagccgtg ggctcgaagg cgagcgcttc
     2281 gaatcgctcg cgcacctcgg gactttgcac tgcgcgcatc accgcgtcgt tcagcctctg
     2341 cgcgatatcg cgcggcgtcg ccgccggcac gaagagtccc acccaggtgt agtcctccat
     2401 gtccgcgtag ccgagctcac ccagcgtcgg cacgtcgggt agcgaggcca ggcggcggct
     2461 cgacgacacc gcgagagcgc gcagctttcc ggccctgagg ttcgccatcg gtccggatgc
     2521 cgccatcgag ccgatcggcg gctgaccgcc cagtaccgcc gcgaccgcag gccctgcgcc
     2581 cttgaacggc acgtgcgtga tgtctacttt ggcacgcacc ttgaagagat tctccgccgt
     2641 gaggtgcggc gtcgtgccgc tggacggcga ggcgtaagcg agcttgccgc tgcgcgccat
     2701 ctgcagcagc tcggcgagcg tttttgccgg aaagtcggct tgcacgacga tcagattcgg
     2761 ctgccaggcg acgatcgcga ccggcacgaa gtccttctgg gggtcgtagc cggggttggg
     2821 cgtgaggctc ggattcaccg cgtaggcgga agaggtggcg agcagcgtgt agccgtcggc
     2881 cggcgccctg gccgcttgag aagtcggaag attgccgccc gcgccggcgc ggttctctac
     2941 cactacgggt tggccgaggt ctgcctgcag cttcggcgcc accagccggg cgatgatgtc
     3001 gaccggtccg cccggcggga acgccaccac tatgcggatt ggtttggagg gataaggctg
     3061 tgccagccct ccagcacaaa acagtgcggt aatgactccc agaaaaatcc gcttcatatt
     3121 tcctcctagc cgctcaccgc ggcgggcgcg atggcctcga tgtcggtgac gacgtcgatg
     3181 atcgccggtt tgccgctgtc gagagctttt tgcaagacgg cgggaaaatc cgccgcgcgc
     3241 tcgacgcgaa tgccgaacgc gccgatatcg gccgcaatgc gcgcaaaatc gaccatgcga
     3301 taggtccaga gctcgcgcgc tttcgcggtc tgctcgccgc cataggcgcg gtcgaagccg
     3361 cgcttggact ggttgccgct agcgttgttg ttgaccaccg tgacggcgcc gatattccag
     3421 cgcactgcgg tctcgatctc gccgaggtga taccagagcc cggcgtcgcc ggtgaaggtc
     3481 acgaccggcc gctgcggtgc ggcacacttt gcgcccagcc cggcggggaa cgcccagccg
     3541 agatggccgg cgctgcgcag gaagctctgc cgcggcgagc gcacgtcgta catgccgccc
     3601 atccacatgc cggcgtgccc ggtgtcggag agcacgatgg cgtcctccgg cacgtggcgg
     3661 ctcagctcgg cgcagatgcg ctcgggacgg atcggtaccg cgtcggactc gagcgcagcg
     3721 cggtatttgt cgcgccactg ctcacaaatg cctttgactt gagaaatcca ggacgttctt
     3781 cgcgaggcgg tcgagcgatc ggcgagcttc agcatctcgg caagcgttgt cttggcatcg
     3841 ccaagcacac cagccctgag cgggtagttg cggccgatga cctcgggctc gatgtcgatc
     3901 tggatcgcgg cgacaccgat cttcggcacg ctccacacgt gcgtcgtcat gccgccggct
     3961 gaagtgccga cgaagcacac cagatcggca gcggccacga cccggttcgc gctctcgcgc
     4021 gagtagctgc cgacgacccc cgcgcacagc gggtgcgagg cggcaatgca atccttgccg
     4081 ttgagcgagg tcacgacggg aatacaaagt gcttccgcga gcgccgcgag ctcgggcccg
     4141 gcgcccgacc agcgcgcgcc gcctcccgcg acgaggactg gccgttccgc tttttgaagt
     4201 atatcgacag cggttttcac ggattgagaa tcgggcacag gccggaacgc cggcgcgcgc
     4261 gcgaactgcg gctcgacctg cggctccatc tcggcttctt cctggtcgat ctggccttcg
     4321 ttgccgcgga actgaaggtg caccgggccc ggggcgccgc tggtggcgac gcggaatgcc
     4381 tggcgcagca tgtccgggaa gcgctcgacg gcgtcgacgc tcgcgttcca cttcgtcacc
     4441 ggctcgaatg ccggcacgtc gtccgcttcc tggtagacct tgcggaactt ggtcttcggg
     4501 tcgcgcccgc cggtgaacgc gagcaccggc gagtgcgcga gccaggcgtc gcgcagcccg
     4561 gcggcgaggt tgagcgcgcc gaccacctgg gccatgcaca cgccgggcct gccgcaggcg
     4621 cgcgcatagc cgtcggccat gtaagcggcg gctttttcac cgtgagtgtg gatgcgcgcg
     4681 atcgaagtgc ggcgttccat ctcggcgaag ctgcggcgca atacggccgg aaccatgaag
     4741 acatgcgaca cgccgtagcc ggagagcatg tccgccagaa cctgagcccc gctcagtttc
     4801 gccactgaat tgttctactt taaggcgaga ggattcgt
//
LOCUS       DASPXW010000409         2394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_872809, whole genome shotgun sequence.
ACCESSION   DASPXW010000409 DASPXW010000000
VERSION     DASPXW010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872809"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..923)
                     /locus_tag="VEL04_09360"
     CDS             complement(99..923)
                     /locus_tag="VEL04_09360"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HYT48618.1"
                     /translation="MKTVLITGATGDVGTHLSRELAGKYKLRLSDRRSLKPSRGQAFI
                     KADISKMSDALRITKGVDAIVHLGGYSVEGPWEAILQANIIGCYNVFEAARRNGVKRI
                     VFPTSNHAMGFYRRDQTIDHRVYIKPDSRYGVSKVFGEALGSLYADKYGMEMFMVRIG
                     NVHPFPLDKRRLSIWFSPRDLAQLISIGIDHPDIRFEIVYGVSGNKRSWYDNSNAVRL
                     GYKPQDDAETLAEEVLKKEKPGDPFAEVYQGGIFTVAEEIPNPAAPKKAAKGRKKK"
     gene            complement(920..1897)
                     /locus_tag="VEL04_09365"
     CDS             complement(920..1897)
                     /locus_tag="VEL04_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012406157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HYT48619.1"
                     /translation="MKILLTHSPEALANYYGERALAGLQAVGEVRLHQRPEPLEGGAL
                     IKAAQDCDLIVSYRQSPGPAQLFERLPSLIAFLRCAIDIRNIDVPAASKAGVLVTQAS
                     AGFVTSVTELVVGFLIDLSRGVTRHATDYHQGRIPKAFMGKELRGSTLGVIGYGAIGR
                     ELVRVAKALGMRVLVTDPYVKDIEQTSFDELLGQSDYVVPLAVATAETENLMNAAAFS
                     KMKKGAYFINVSRGNLVDEAALEAALESGRLAGCAIDVGRAPDQMPTPRLAARSDVIA
                     TPHTAGLTLPAIEHQSMETVAQAAEIAKGRAPKGSVNAEHWKRKELLKK"
     gene            complement(1894..>2394)
                     /locus_tag="VEL04_09370"
     CDS             complement(1894..>2394)
                     /locus_tag="VEL04_09370"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HYT48620.1"
                     /translation="EPQRAPFRIEPERMQTIADILNVMELRLGVEIESAGLAAERASR
                     AQLRAIGAAFDTIGRAAAAGKSAVDEDLGFHRAIAEATGNPEFARFLQFIGGHLIPRR
                     TVSGLPERMGGARAYLALIQEEHRRIWQAIESRDAKAAREAMRRHLTRSLERYRKLAA
                     EQKAAA"
BASE COUNT          375 a          771 c          776 g          472 t
ORIGIN      
        1 caggaaggta cccggcgcct ggctgccctc cttgccgtag aaatgcatgt gcgtgtcgca
       61 ggcgccgcgc ggcgccttga gcttcggctt cttgtcgctc atttcttctt cctgcctttc
      121 gccgctttct tcggcgctgc gggattcgga atctcctcgg cgacggtgaa aatcccgccc
      181 tgatagacct cggcgaacgg atctccgggt ttttcctttt tcaaaacctc ttcggcaagc
      241 gtttccgcgt catcctgcgg cttgtagccc aggcgcacgg cgttggagtt gtcgtaccag
      301 ctgcgcttgt tgcccgagac gccgtacacg atctcgaacc tgatgtcggg atgatcgatg
      361 ccgatcgaga tcagctgggc gaggtcgcgc gggctgaacc agatcgacag gcgccgcttg
      421 tcgagcggga acggatgcac gttgccgatg cgcaccatga acatctccat gccgtacttg
      481 tcggcataga ggctgcccag ggcttcgccg aataccttgg agacgccgta gcggctgtcg
      541 ggcttgatat agacgcggtg atcgatggtc tggtcgcgcc ggtagaagcc cattgcgtga
      601 ttgctggtgg ggaacacgat gcgctttacc ccgttccggc gggctgcttc gaacacgtta
      661 tagcagccga tgatgttcgc ctggaggatc gcctcccagg ggccttcgac cgaatagccg
      721 ccgaggtgga cgatcgcgtc gacgcccttg gtgatgcgca gcgcgtcgga catcttggag
      781 atgtccgcct tgatgaaagc ctgccccctg gaaggcttga gggagcgcct gtccgacagc
      841 cgcagcttgt acttgccggc cagctcacga ctgaggtggg tgccgacgtc cccggtcgcg
      901 ccggtgatca gaacggtttt catttcttca gcagctcctt tcttttccag tgctcggcgt
      961 tgaccgatcc cttgggcgcg cgtcccttgg cgatttccgc cgcctgcgcc accgtctcca
     1021 tcgattggtg ctcgatcgca ggaagcgtga ggcctgccgt gtgcggcgtc gcgatcacgt
     1081 cggaccgcgc cgcgaggcga ggggtcggca tctggtcagg cgcacgcccg acgtcgattg
     1141 cgcatcccgc gagccgcccc gattcgagcg ccgcttccag cgccgcctcg tcgacgaggt
     1201 tgccgcgaga gacattgatg aagtacgcac ctttcttcat cttcgagaac gccgcggcgt
     1261 tcatgagatt ttcggtttcg gcggtggcga cggcgagcgg tacgacgtag tcggattgcc
     1321 ccagaagttc atcgaacgac gtctgctcga tgtccttcac ataggggtcc gtgacgagaa
     1381 cgcgcattcc cagtgccttc gccaccctga ccaattcccg gccgatggcg ccgtagccga
     1441 tgacgccgag cgtgctgcca cgcagctctt tgcccatgaa tgccttcggt atcctgcctt
     1501 gatggtagtc cgtcgcatgg cgcgttacgc ctcgcgaaag atcgatcaaa aaaccgacta
     1561 ccagctcggt gaccgaagtg acgaagcccg cgctcgcctg agtgacgaga acgcctgcct
     1621 tgctcgccgc ggggacgtcg atattgcgga tgtcgatggc gcagcgcagg aaggcgatga
     1681 ggctcggcag gcgctcgaac agctgtgccg ggcccggcga ctggcgatac gacacgatga
     1741 gatcgcagtc ctgcgcagcc ttgatgagcg ctccaccctc aagaggttca gggcgttgat
     1801 gaagccttac ttcgccaacg gcttgcaaac ccgccagcgc gcgctcgccg taatagttgg
     1861 cgagcgcctc gggcgagtgc gtcagcaata tcttcaagca gctgcttttt gctcggcggc
     1921 gagcttgcgg tagcgctcca gcgatcgcgt cagatggcgg cgcatcgcct cgcgcgccgc
     1981 cttcgcatcg cggctctcga tcgcctgcca gatcctgcgg tgctcctcct ggatcagcgc
     2041 caggtaggca cgcgcgccac ccatgcgctc gggcagcccg ctcaccgtgc ggcgcgggat
     2101 caggtggccg ccgatgaact gcaggaagcg cgcgaactcc gggttgccgg tggcttccgc
     2161 aatggcgcgg tgaaagccga ggtcctcgtc gaccgccgac ttgccggcgg cggcggcgcg
     2221 cccgatggtg tcgaacgcgg cgccgatcgc gcgcagctgc gcgcgcgagg cgcgctcggc
     2281 ggcgagcccg gccgactcga tctcgacgcc gagccgcagc tccatcacgt tgaggatgtc
     2341 ggcaatcgtc tgcatgcgct cgggctcgat gcggaacggc gcgcgttgcg gctc
//
LOCUS       DASPXW010000410         3750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1506925, whole genome shotgun sequence.
ACCESSION   DASPXW010000410 DASPXW010000000
VERSION     DASPXW010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1506925"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="VEL04_09375"
     CDS             <1..1005
                     /locus_tag="VEL04_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48621.1"
                     /translation="LLLRDLAIFEGGRQLSERGAEGSGPRVVGIYVGWRGESITLPIV
                     NTVTFWERKNTAERVAQGSVRELFARLDFFRDRASADGKRNVRLLTIGHSFGGLITFE
                     ALSSEFLRAAVRSGGGYVSRIGDVVVIVNPAVEGARYEPLMVAAQRMSGLKDNQLPVV
                     ILATSTADSATGFFFPLARNLSTLLESTPGPEGDAIVKAVGHNERYITHKLSLCGAED
                     KEDCAAACPAPRLKERQSVKAMSSTDQEDYSSKLRQDMAAEVALMRVIGDKGFKGRKY
                     LCSGMRLEPTVKQVPEQNPFWVVSTTGDIMGGHNDIFNPKFVSFVRQMYLGFIAERFK
                     "
     gene            1083..1589
                     /locus_tag="VEL04_09380"
     CDS             1083..1589
                     /locus_tag="VEL04_09380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01926.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HYT48622.1"
                     /translation="MRHILHMNKPVGRALIQLHEALMRAGSPLTPAQHELIAAYVSGL
                     NACHYCYGVHSRTAEAFGVSEKTLQALVADLNAAPVEAKMKPLLAYARKLTLTPAKMT
                     QADAKAAFDAGWSERALHDAVLTTSLFNFMNRLVDGHGCTGSEDIFKSRGRALKDSGY
                     APLLKFLE"
     gene            complement(1609..2028)
                     /locus_tag="VEL04_09385"
     CDS             complement(1609..2028)
                     /locus_tag="VEL04_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48623.1"
                     /translation="MSRITLAVILAVVTFSIGAQPVKCVDASGKVRYIDESMMGQEKC
                     KPVRAEVQFVPAAPGAAPSAKPPGSASPGSAAEREARLKDAQNRLADARKQLAEQEAV
                     RGGDERNYARVEERLKPYQEAVQAAEQEVEQARRAFR"
     gene            complement(2041..>3750)
                     /locus_tag="VEL04_09390"
     CDS             complement(2041..>3750)
                     /locus_tag="VEL04_09390"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008342453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HYT48624.1"
                     /translation="LVGLPVALDGLLYNCAALICQGRLVGVVPKTYLPNYREFYEARQ
                     FTPGDASRSSEIELAGQTAPFGTGLLFKLKEIQGFLLHVEICEDLWVPAPPSSFAAMA
                     GATVIANLSASNIVIGKEGYRHQLAGNQSARCLAAYLYSAAGRGESTTDLAWDGHAMI
                     YENGTLVAESRRFADAPQLAVGDVDLGRLLADRMRQNTFGGAALRHAAEVARFRRIEF
                     SVPIPAGRLALERKIERLPYVPSDAANRDARCEEVYRIQVQGLATRMRATGTKKLVIG
                     VSGGLDSTQALIVCARAMDELKLPRRNVLACTMPGFATSSRTRNQAWQLMRAVGATAE
                     EIDIRPSCMQMLKDLRHPYAKGRKVYDITFENVQAGERTSHLFRLANLHQGLVVGTGD
                     LSELALGWATYGVGDHMSHYNVNVSVPKTLIQYLIGWVADSDEFSADVKRALHRVLDT
                     EISPELVPGKQRTEASVGPYELQDFNLYYTLRFGYSPAKVAFLAWHAWKGKYRLAEIR
                     KWLGVFLKRFFQSSQFKRSALPNGPKVGSGGSLSPRGDWRAPSDGNADAWLADLARVP
                     RGE"
BASE COUNT          604 a         1196 c         1322 g          628 t
ORIGIN      
        1 ctgctgctgc gcgacctcgc gatcttcgag ggcggcaggc agctctcaga gcgcggcgcc
       61 gaaggctcgg ggccgcgcgt ggtcggcatc tatgtgggct ggcgcggcga atccatcacg
      121 ctgccgatcg tcaacaccgt caccttctgg gagcgcaaga acaccgcgga gcgcgtggcg
      181 caggggtcgg tgcgcgagct cttcgcgcgg ctggattttt tccgggaccg ggcaagcgcc
      241 gacggcaagc gcaacgtgcg cctgctcacc atcgggcatt ccttcggcgg gctgatcacc
      301 ttcgaagcgc tgagcagcga gttcctgcgc gcggcggtgc gcagcggggg cggttatgtc
      361 tcgcgcatag gcgacgtcgt ggtgatcgtc aacccggcgg tcgagggcgc gcgctacgag
      421 ccgctgatgg tggccgcgca gcgcatgagc ggcctgaagg acaaccagct tccggtggtg
      481 atcctggcga cctcgaccgc cgacagcgcg accggattct tctttccgct ggcgagaaac
      541 ctcagcacgc tgctcgagag cacgccgggg cccgaaggcg acgccatcgt gaaggccgtg
      601 ggccacaacg agcgctacat cacgcacaag ctttcgctgt gcggggcgga ggacaaggag
      661 gactgcgccg ccgcatgccc ggccccgcgc ttgaaggagc gccagtccgt caaggcaatg
      721 agcagcacgg atcaggaaga ctactccagc aagctgaggc aggacatggc cgccgaggtc
      781 gcgctgatgc gggtgatcgg cgacaagggc ttcaagggaa ggaaatatct gtgcagcggc
      841 atgcgcctcg agcctacggt gaagcaggtg cccgagcaga atccgttctg ggtagtttcc
      901 accaccggcg acatcatggg cggccacaac gacatcttca atccgaagtt cgtctccttc
      961 gtccgccaga tgtacctcgg cttcatcgcc gagcggttca agtagccgag ttagcgcggt
     1021 aacacaagcc cgcctgggag atcgcaatgc cctttttcaa atcactgcca aaggactgtg
     1081 gcgtgcgcca catcctgcac atgaacaagc ccgtcgggcg ggcgctgatc cagctccatg
     1141 aggcgctgat gcgcgcgggg tcgccgctga cgccggcgca gcacgagttg atcgcggcgt
     1201 acgtgtcggg gctgaacgct tgccactact gttacggggt gcactcgcgg actgcggagg
     1261 cgttcggcgt gtcggagaag acgctgcagg cgctggtggc ggacctgaac gcggcgccgg
     1321 tcgaggcgaa gatgaagccg ctgctcgcct acgcgaggaa gctcacgctg acgccggcca
     1381 agatgaccca ggcagatgcc aaggcagcgt tcgacgccgg ctggagcgag cgcgcgctgc
     1441 acgacgcggt gctcaccacg agcctgttca atttcatgaa ccgcctggtt gacggccatg
     1501 gctgcaccgg cagcgaggac atcttcaaga gccgcgggcg cgcgctgaag gattcgggtt
     1561 acgcgccgct gctgaagttt ctggaataaa tcgggctctg accccgattt agcggaacgc
     1621 ccttctggcc tgctcgactt cttgttccgc ggcctgcacc gcttcctggt agggcttcag
     1681 gcgctcctcg acgcgcgcgt agttgcgctc gtcgccgccg cggaccgctt cctgctcggc
     1741 gagctgtttc ctggcatcgg cgagcctgtt ttgcgcgtcc ttcaggcgcg cttcgcgctc
     1801 ggcggcgctg cctgggcttg ccgagcccgg cggcttggca gaaggcgcgg cgccgggcgc
     1861 cgcaggcacg aattgcactt ccgcccgcac cggtttgcat ttctcctgcc ccatcatgct
     1921 ctcgtcgatg tagcgaacct tgccggacgc gtcgacgcat ttcactggct gcgcgccgat
     1981 gctgaaggtc acgaccgcca ggattactgc cagcgtgatg cggctcatgg tgatcggatt
     2041 ttattcccct ctgggcacgc gcgcgaggtc ggcgagccag gcatcggcat tgccatccga
     2101 gggggcgcgc cagtcgccgc gcggcgagag cgagccgccc gagcccacct tggggccgtt
     2161 cggcagggcg ctgcgcttga actggctcga ctggaagaag cgcttgagga agacgccgag
     2221 ccatttgcgg atctcggcga gccggtattt ccccttccag gcgtgccagg cgaggaacgc
     2281 caccttggcg ggcgagtagc cgaagcgcag ggtgtagtag aggttgaagt cctgcagctc
     2341 atagggcccg acgctggcct cggtgcgctg cttccccggg acgagttcgg gcgaaatttc
     2401 ggtatcgagc acgcgatgca gcgcgcgctt gacgtcggcg ctgaactcat cggagtcggc
     2461 gacccagccg atgaggtact ggatcagcgt cttcggcacg ctcacgttca cgttgtaatg
     2521 cgacatgtgg tcgccgacgc cgtaggtcgc ccagccgagc gcgagctcgg acaggtcgcc
     2581 ggtgccgacc accaggccct ggtgcaggtt ggcgagccgg aacaggtggc tggtgcgctc
     2641 gcccgcctgc acgttctcga aggtgatgtc atagaccttg cggcccttgg cgtagggatg
     2701 gcgcaggtct ttcagcatct gcatgcacga gggccggatg tcgatttcct ccgcggtggc
     2761 gccgaccgcg cgcatcagct gccaggcctg gttgcgcgtg cggctgctgg tggcgaaccc
     2821 gggcatggtg caggcaagga cgttgcgccg ggggagcttc agctcgtcca tggcgcgcgc
     2881 gcacacgatc agcgcctggg tcgagtcgag cccgcccgag acgccgatca cgagcttctt
     2941 cgtcccggta gcgcgcatgc gcgtggcgag cccctgcacc tggatgcggt agacctcttc
     3001 gcagcgcgca tcgcggttcg ccgcatcgct cggcacgtaa ggaagccgtt cgatcttgcg
     3061 ttcgagcgcg aggcggccgg ccgggatggg aacggaaaac tcgatgcggc gaaagcgcgc
     3121 cacctccgcg gcgtggcgca gcgccgcgcc accgaaggtg ttctggcgca tgcggtcggc
     3181 gagcagtcgc ccgaggtcga cgtcgccgac ggcgagctgc ggtgcgtcgg cgaagcgccg
     3241 cgattcggcg accagggtgc cgttctcgta gatcatggcg tggccgtccc aggcgagatc
     3301 ggtggtcgac tcgccgcgcc cggccgcgct gtagaggtag gccgcgaggc agcgcgccga
     3361 ctggttgccg gcgagctggt ggcggtagcc ctccttgccg atgacgatgt tcgaggccga
     3421 gaggttggcg atcacggtcg cgcccgccat cgcggcgaac gaggagggcg gcgccggcac
     3481 ccagaggtct tcgcaaatct cgacgtgcag caggaaccct tgaatttctt tcagcttgaa
     3541 aagcagcccg gtgccgaagg gcgcggtttg tccggcgagc tcgatctcgc tcgagcgcga
     3601 cgcatcgccc ggcgtgaact ggcgcgcctc gtagaactcg cggtagttcg gcaggtaggt
     3661 cttcggcacc acgccgacga ggcggccctg gcagatgagc gccgcgcagt tgtagagcag
     3721 gccatcgagc gccacgggaa ggccgacgag
//
LOCUS       DASPXW010000411         5275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_625326, whole genome shotgun sequence.
ACCESSION   DASPXW010000411 DASPXW010000000
VERSION     DASPXW010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5275
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625326"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /gene="cobA"
                     /locus_tag="VEL04_09395"
     CDS             complement(<1..598)
                     /gene="cobA"
                     /locus_tag="VEL04_09395"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HYT48625.1"
                     /translation="MGSVPISGTVYLVGAGPGAPDLLTLRAARLLGEAEIVFHDALVP
                     EEILLLASRAEKVAVGKRSGRHSTAQRFINKRLLDAARRYRVVVRLKGGDPMLFGRAH
                     EEISFLMNKGIQVEVVPGVTAALAASAELQVSLTRRGAARSVAFVTPRVGAGERASSW
                     VQALLAADTAAIYMGAGEAAQIAAALIAAGKPGATPVAI"
     gene            complement(599..1000)
                     /locus_tag="VEL04_09400"
     CDS             complement(599..1000)
                     /locus_tag="VEL04_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010102143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HYT48626.1"
                     /translation="MATLIKGRRVISESAPPPEVGEVIRLEPADDPASVADRLDRVAR
                     IEVSFPKFGDGRGYSIARLLRERYGYKGELRAVGQITRDLLFFMESCGFDAFELREGE
                     DPHEALAAFEDFSESYQASVKRPVPLFRRRQ"
     gene            complement(945..2651)
                     /locus_tag="VEL04_09405"
     CDS             complement(945..2651)
                     /locus_tag="VEL04_09405"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HYT48627.1"
                     /translation="MYRYDEIDQRLVDERVRQFRDQTRRFLAGQLSEEEFRALRLRNG
                     LYIQRYAPMLRISIPYGLLSSRQLRKLSAIARKYDRAYGHFSTRQNLQLNWPKLEQAP
                     DILAELATVQMHAIQTSGNSFRNVTTDHFAGVAQDEIVDSFVWCEIIRQWSTFHPEFS
                     YLPRKFKIAVNGAKADRAAVAVHDIGLHAITNDANQVGFKVLVGGGLGRTPMIGFVIR
                     EFLPWQHLLSYLEAILRVYNRYGRRDNIHKARIKILVRERGPEKFRDEVEAEWAHLKD
                     GPATLIAGEIERVAARFTRPGYEELSNRNLSTGGPFGSWIKRNVQPHKVPGYAAVTLS
                     LKKTGVPPGDVTSDQMDAIADLADRYSFGELRVTHEQNLVLADVRQSDLGELWREARA
                     LGLATPNLGLLTNIIACPGGDFCALANAKSIPVAEAIQRRFDDLDYLHDIGELDLNIS
                     GCMNACGHHHVGHIGILGVDKNGEEWYQIEIGGNQGKTQTGEAAPGASLGKVIGPSFA
                     RAQVPNVIERLIDCYLEKRDSEAERFVEVVHRIGVEPFKESVYGNADQRASRYQRERA
                     AA"
     gene            2727..3671
                     /locus_tag="VEL04_09410"
     CDS             2727..3671
                     /locus_tag="VEL04_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HYT48628.1"
                     /translation="MKLQQLRCLLEVARRGLNVSEAAEALHTSQPGVSKQIRALEDEL
                     GVQVFVRHGKRLVAVPEPGKAVIGIAERILSEAQNLRRAGEEYANDQLGTLTIAATHT
                     QARYALPKAVAAFKRRYAKVELVIHQGNPTQICEQVLAGEADMGVATEMIALYPELIS
                     LPVYQWNRCVVVPPKHPLLKAVPLTLEKLAEYPIVTYDFAFANRSLVQKAFESRGLNP
                     HVVLSAQDSDVIKTYVELGLGVGILAKMAFDAKRDFTLRAIDASHLFESSTTRLGIKR
                     GAYLRRYAYEFIELLAPHLPRAIIESAVTGQEGSRYEL"
     gene            complement(3668..4876)
                     /gene="phaZ"
                     /locus_tag="VEL04_09415"
     CDS             complement(3668..4876)
                     /gene="phaZ"
                     /locus_tag="VEL04_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HYT48629.1"
                     /translation="MLYSLYEAQHTALAPLRFWAEFSHGWFGHPFSPFIYLPLARRVA
                     ASSDLFLRLTGRYEKPKWNIECATIEVALDKPFCKLIHFKQEIPKHRKVLVVAPLSGH
                     HATLLRDTVRSLLPEHDVWVTDWVDARMVPLSAGPFHLADYVDYVREWIRLLSPDLHV
                     ISVCQPTVPVLAAVSLMACNREPAPLSMVMMGGPIDARRSPTSVNNLARRKPYSWFEQ
                     NLIHRVPPKYPGYMRRVYPGFLQHLGFVAINPDRHLIAHWDYYHRLMQGNGESAEAHR
                     RFYDEYNAVLDMPAEYYLDTVKTVFQDYALPRGRMFVREELVRPQAIRDTALLTVEGE
                     LDDISGHGQTEAAHALCLNIPRGRREHFVAPGVGHYGIFSGRRWREMVFPRVRDFIEK
                     NAKKMGTDPI"
     gene            complement(5034..>5275)
                     /locus_tag="VEL04_09420"
     CDS             complement(5034..>5275)
                     /locus_tag="VEL04_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018310601.1"
                     /note="activates fatty acids by binding to coenzyme A;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HYT48630.1"
                     /translation="PGVLECAVIGVPDEHSGEVPKVFVVKKDPQLTGQDILEYCKKEL
                     TAYKRPKYVEFRSELPKTNVGKILRRALRDEKKAA"
BASE COUNT          892 a         1717 c         1739 g          927 t
ORIGIN      
        1 cgattgcgac cggcgtcgcg ccgggcttgc ccgcggcgat cagcgccgcg gcgatctgcg
       61 ccgcttcccc tgctcccatg tagatggcgg cggtgtcggc ggcgagcagc gcctggaccc
      121 agctgcttgc gcgctcgccc gcgccgaccc ggggcgtgac aaaggctacg ctacgcgcgg
      181 cgccgcggcg ggtgagcgag acctgcagct cggcgctcgc ggcgagggca gcggtgacac
      241 cgggaaccac ctctacttga atgcccttgt tcatcaaaaa ggaaatttcc tcgtgagcgc
      301 ggccgaagag catcgggtcg ccgcccttga ggcgcaccac cacgcggtag cggcgcgcgg
      361 cgtccagcag gcgcttgttg atgaagcgct gcgcggtcga gtgccggccg gagcgcttgc
      421 cgacggcgac tttctccgct ctcgaagcta gaagaagtat ttcttcaggt accagggcgt
      481 cgtggaacac gatctcggcc tcgccgagca gccgggccgc gcgcagcgtg aggaggtcgg
      541 gcgcgcccgg cccggcgccg acgagataga ccgttcccga aatggggaca gaccccattt
      601 attgccggcg gcggaaaagc ggcacgggac gcttcaccga cgcctgatac gactcgctga
      661 aatcttcgaa cgcggcgagc gcctcgtgcg gatcctcgcc ctcgcgcagc tcgaaggcgt
      721 cgaagccgca gctttccatg aagaacagga ggtcgcgcgt gatctgcccc actgcgcgca
      781 gctcgccctt gtagccgtag cgctcgcgca gcaggcgcgc gatcgagtag ccgcggccgt
      841 cgccgaactt cgggaagctg acttcgatgc gcgccacgcg gtcgagccgg tccgcgaccg
      901 acgccggatc gtcggccggc tcgaggcgga tcacctcgcc gacctcaggc ggcggcgcgc
      961 tctcgctgat aacgcgacgc cctttgatca gcgttgccat agacactttc cttgaaaggc
     1021 tcgactccga tgcggtgcac gacctcgacg aagcgctcgg cctcgctgtc gcgcttttcg
     1081 agatagcagt cgatcaggcg ctcgatcacg ttcggcacct gcgcgcgcgc gaacgagggg
     1141 ccgatgactt tgccaagcga cgcgcccgga gcggcctcgc ccgtctgcgt tttcccttga
     1201 ttgccgccga tctcgatctg gtaccactcc tcgccgttct tgtcgacgcc gaggatgccg
     1261 atgtggccga cgtggtggtg gccgcatgcg ttcatgcagc ccgagatgtt gaggtcgagc
     1321 tcgccgatat cgtgcaggta gtcgagatcg tcgaagcggc gctggatcgc ttccgcgacc
     1381 gggatcgact tggcgttggc gagcgcgcag aaatcgccgc ctgggcaggc gatgatgttg
     1441 gtgagcaggc cgaggttcgg cgtcgccagc ccgagcgcgc gcgcctcgcg ccacagctcg
     1501 ccgaggtcgc tctggcgcac atcggcgagc accaggttct gctcgtgcgt cacgcgcagc
     1561 tcgccgaacg aatagcggtc ggcgagatcg gcgatggcgt ccatctggtc ggaggtgacg
     1621 tcgcccggcg gcacgccggt cttcttcagc gacagcgtca cggctgcgta gcccggcacc
     1681 ttgtgcggct ggacattgcg tttgatccag gagccgaaag gtcctcctgt tgaaagattc
     1741 cggtttgaca gttcctcgta cccgggacgc gtgaaacggg ccgcgacccg ctcgatttct
     1801 ccggcaatca gcgtcgccgg gccgtccttg aggtgcgccc actcggcctc gacctcgtcg
     1861 cggaacttct ccgggccgcg ctcccttacc aggatcttga tgcgcgcctt gtggatgttg
     1921 tcgcggcgcc cgtagcggtt gtagacgcgc aggatcgcct cgagatagct gagcaggtgc
     1981 tgccacggca ggaactcgcg gatgacgaag ccgatcatcg gcgtgcggcc gagcccgccg
     2041 cctacgagta ccttgaaacc gacctggttg gcgtcgtttg tgatcgcgtg cagcccgatg
     2101 tcgtgcaccg ctaccgcggc gcggtcggcc ttcgcgccgt tcaccgcgat cttgaacttg
     2161 cgcggcaaat aggaaaactc gggatggaag gtcgaccact ggcggatgat ctcgcaccag
     2221 acgaaggaat cgacaatctc gtcctgcgcc acgccggcga aatggtcggt ggtgacgttg
     2281 cggaacgagt tgcccgaggt ctggatggcg tgcatctgca cggtggcgag ctcggcgagg
     2341 atgtcgggcg cctgctcgag cttcggccag ttgagctgca ggttctggcg cgtcgagaag
     2401 tggccgtagg cgcggtcgta cttgcgcgcg atcgcggaga gcttccttag ctggcggctc
     2461 gagagtagcc cgtacgggat cgagatgcgc agcatcggcg cgtagcgctg gatgtacagg
     2521 ccgttcctca gccgcagcgc gcggaactcc tcctcggaga gctggcccgc gaggaagcgc
     2581 cgcgtctggt cgcggaactg ccgcacgcgc tcgtccacca ggcgctggtc gatttcgtcg
     2641 tagcggtaca cggtcgggaa atcctagctc ccggccttat tccctcaaag acgattatgt
     2701 taggatgtta ttcatataag acatatatga agctgcagca gctgcgctgc ctgctcgaag
     2761 tcgcccgccg cggcctcaat gtttccgagg ccgccgaggc actgcatacc tcgcaacccg
     2821 gggtctcgaa gcagatccgc gccctcgagg acgagctcgg cgtgcaggtg ttcgtgcgcc
     2881 acggcaagcg cctggtcgcg gtgcccgagc ccggcaaggc agtgatcggc atcgccgagc
     2941 gcatcctctc cgaggcccag aacctgcgcc gcgcagggga ggaatacgcg aacgatcagt
     3001 tgggcacttt aacgatagcg gccactcaca cccaagcccg ttacgcgctt cccaaggccg
     3061 tggcggcgtt caagcggcgc tatgccaagg tcgagctcgt catccaccag ggaaacccga
     3121 cccagatctg cgagcaggtg ctggccggcg aggccgacat gggcgtcgca accgagatga
     3181 ttgcgctcta tcccgagctg atctcgctgc cggtctacca gtggaaccgc tgcgtggtgg
     3241 tgccgccgaa gcacccgctg ctcaaggccg tgccgctcac gctggagaag cttgccgagt
     3301 atcccatcgt gacctacgac ttcgccttcg ccaaccgctc gctggtgcag aaggcgttcg
     3361 agagccgggg cctcaatccc cacgtggtgc tgagcgcgca ggattcggac gtcatcaaga
     3421 cctatgtcga gctcggcctg ggggtcggca tcctcgccaa gatggcgttc gatgcgaagc
     3481 gcgacttcac cctgcgcgcg atcgacgcca gccacctgtt cgaatcgagc accacgcggc
     3541 tcggcatcaa gcgcggcgcc tacctgcgcc gctacgcgta cgagttcatc gagctgctcg
     3601 cgccgcatct gccgcgcgcc atcatcgaga gcgcggtcac cgggcaggaa ggctcgcgct
     3661 acgaacttta aatggggtct gtccccattt ttttcgcatt tttttcgatg aagtcccgca
     3721 ccctcggaaa caccatctcc cgccagcggc gccccgagaa aatgccgtag tgcccgacgc
     3781 cgggcgcgac gaagtgctcg cgccgcccgc gcggaatatt gaggcacagc gcgtgtgccg
     3841 cctcggtctg gccgtggccg gaaatgtcgt cgagctcgcc ttcgaccgtg agcagcgccg
     3901 tgtcgcggat ggcctgcggc cgcaccagct cctcgcgcac gaacatcctg cccctcggca
     3961 gcgcgtaatc ctggaacacg gttttcacgg tatcgaggta atactcggca ggcatgtcga
     4021 gcaccgcgtt gtactcgtcg tagaagcgcc ggtgcgcttc cgcggactcg ccgtttccct
     4081 gcatcaggcg atggtagtaa tcccagtgcg cgatcaagtg gcggtcaggg ttgatcgcca
     4141 cgaagccgag gtgctgcagg aacccgggat agacgcgccg catgtagccc ggatatttcg
     4201 gcggcacgcg gtggatcagg ttctgctcga accacgaata cggcttgcgc cttgccaggt
     4261 tgttgaccga ggtcgggctg cgccgggcgt cgatcggccc gcccatcatc accatgctga
     4321 gcggcgccgg ctcgcggttg cacgccatca gcgatacggc ggcgagcacg ggcaccgtcg
     4381 gctggcagac cgagatgacg tgcaggtccg gcgacagcag ccggatccac tcgcgcacgt
     4441 aatcgacgta atcggcgaga tgaaaaggtc ccgcggagag cggcaccatg cgcgcatcga
     4501 cccaatcggt gacccagacg tcgtgctcgg gcagcagcga gcgcacggtg tcgcgcagca
     4561 gcgtcgcgtg gtggccggag agcggcgcca ctaccaggac tttcctgtgc tttggaattt
     4621 cctgcttgaa gtggatcagc ttgcagaagg gcttgtcgag cgcgacctcg atggtggcgc
     4681 actcgatgtt ccacttcggc ttttcgtagc gccccgtcag ccgcaggaac agatcgctcg
     4741 aggcggcgac gcggcgcgcc agcggcaggt agatgaaggg actgaacggg tgcccgaacc
     4801 agccgtgcga aaactcggcc cagaagcgca gcggcgcgag cgcggtgtgc tgcgcttcgt
     4861 acaggctgta gagcaccgga aggcctccca cggttcaacg taacccgttg cttataacag
     4921 aattgccgca ttgccgcatt gaactatcgc acagcggcag caggcgttca gccaccgctt
     4981 atagggccgc acaaagaaag gttctaaatg agaaaaatgg ggtctgtccc catttacgcg
     5041 gctttctttt cgtcgcgcag cgcgcgccgc aggatcttgc cgacgttggt cttcggcagc
     5101 tcggagcgga actcgacgta cttcgggcgc ttgtaggcgg tcaactcctt cttgcagtac
     5161 tcgaggatgt cctgcccggt gagctgcgga tccttcttca ccacgaacac tttcggcacc
     5221 tcgcccgagt gctcgtcggg cacgccgatc accgcgcact cgagcacgcc ggggt
//
LOCUS       DASPXW010000412         2506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_122829, whole genome shotgun sequence.
ACCESSION   DASPXW010000412 DASPXW010000000
VERSION     DASPXW010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122829"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VEL04_09425"
     CDS             <1..440
                     /locus_tag="VEL04_09425"
                     /inference="COORDINATES: protein motif:HMM:NF019275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HYT48631.1"
                     /translation="PVSAHTGRNLPELLRVLRAALPEGAAAYPPEQLTDRDERFFAAE
                     LLREKLFEELGQELPYCCEVMIESFKEEGRLRRIEATILVERASQKAIVLGTAGARLK
                     RMASAARNDMERMFGGKVYLGTWVKVRRAWTDDARVLRQLGYE"
     gene            459..1169
                     /gene="recO"
                     /locus_tag="VEL04_09430"
     CDS             459..1169
                     /gene="recO"
                     /locus_tag="VEL04_09430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HYT48632.1"
                     /translation="MKRRADHEPGYVLHTYAYKETSLIVEAFTRRFGRISLLARGARR
                     PRSAMRGVLLSFQALRLTWSASAELGTLIGAEWAGALQPLAGRGLMCGFYLNELVLRL
                     VPREDPHEALFDVYGEALARLASQDLSSVLRGFEKRLLAELGYAPLLVHDSMSRPINP
                     DAQYVYEPDRGPVPTASPGSGALVVSGRTLLDVAADDYARAETRDEARALMRVLIGER
                     LHGQVLHTRSVLMELNEL"
     gene            1180..1926
                     /locus_tag="VEL04_09435"
     CDS             1180..1926
                     /locus_tag="VEL04_09435"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HYT48633.1"
                     /translation="MIELGVNVDHVATVRQARRTYEPDPVWAAVEAHLGGADGITVHL
                     REDRRHIQDEDVRRLRELTQIRLNLEMAATAEMVDVACRVKPEMAMLVPEGRHEITTE
                     GGLDIVSQEKRLIAVVARLASAGIVVSVFIDAEVAQVEAAKRIGAAVCEVHTGPYAHA
                     FHSRGRDAGSPVVISELKKIQTAGELIRSLGMRFNAGHALNYVNVEPVARLQGLRELH
                     IGHAIVSRALFVGMREAVREMKSLMVQAAR"
     gene            1923..2306
                     /gene="acpS"
                     /locus_tag="VEL04_09440"
     CDS             1923..2306
                     /gene="acpS"
                     /locus_tag="VEL04_09440"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HYT48634.1"
                     /translation="MIYGVGTDVVEIQRIEKALARFGERFAQRILCRSELQRFQGHRR
                     KASYLAKRFAAKEAFSKALGTGIHAPANWHGVWVKNLESGKPVFDFSRPLKKLLKSKG
                     VTRAHLSLSDERGVAVATVILECEK"
     gene            2327..>2506
                     /locus_tag="VEL04_09445"
     CDS             2327..>2506
                     /locus_tag="VEL04_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HYT48635.1"
                     /translation="MLDVAGTALGDADRRRLAHPATGAVILFSRNYENPEQLKALTEE
                     IERLRGPALPVCVDHE"
BASE COUNT          449 a          786 c          872 g          399 t
ORIGIN      
        1 ttccggtgag cgcgcacacg ggaaggaacc tgcccgagct gctgcgcgtg ctgcgcgcgg
       61 cgcttcctga aggagcggcc gcctatcccc cggagcagct caccgaccgc gacgagcgct
      121 tcttcgccgc cgagctgctg cgcgagaagc tgttcgagga gctggggcag gagctgccct
      181 actgctgcga ggtgatgatc gagagcttca aggaggaggg caggttgcgg cgcatcgagg
      241 cgacgatcct ggtcgagcgg gcgagccaga aggcgatcgt gctgggaacc gccggcgcgc
      301 gcctcaagcg catggcgagc gcggcgcgca acgacatgga gcgcatgttc ggcggcaagg
      361 tttatctcgg cacctgggtg aaggtgcgcc gcgcctggac cgacgatgcg cgcgtgctgc
      421 gtcagctggg gtatgagtaa taaaggcaca agggcagaat gaaaaggcgc gccgaccacg
      481 aacccggcta cgtgctgcac acgtatgcct acaaggaaac cagcctgatc gtcgaggcat
      541 tcacgcgccg cttcgggcgc atctcgctgc tcgcccgcgg cgcacggcgg ccgcgctcgg
      601 cgatgcgcgg cgtgctgctg tcgtttcaag ccctgcgcct cacctggagc gcctccgccg
      661 agctcggcac gctgatcggc gctgagtggg ctggggcgct gcagccgctg gccgggcgcg
      721 ggctgatgtg cggcttttac ctgaacgagc tggtgctcag actggtgccc cgcgaggacc
      781 cgcatgaagc attgtttgac gtatatggtg aagcgctcgc gcgccttgct tcacaggacc
      841 tctcatcggt actgcgcggc ttcgagaaac gcctgctcgc cgagctcggc tatgctcccc
      901 ttctggttca tgactcgatg agccgcccca taaatcccga cgcgcaatac gtctacgagc
      961 ctgaccgggg gccggtgccg acggccagcc cggggtccgg agcgctcgtc gtcagcgggc
     1021 ggacgctgct cgacgtcgcc gccgacgact acgcgcgcgc cgagacgcgc gacgaggcgc
     1081 gcgctctgat gcgcgtgctg atcggcgagc ggctgcacgg gcaggtgctg catacccgaa
     1141 gcgtcctcat ggagctgaac gagctgtgat cgcgcatcta tgatcgaact gggtgtgaac
     1201 gtcgaccacg tcgccacggt gcggcaggcg cggcgcacct acgagcccga cccggtgtgg
     1261 gcggcggtgg aagcgcatct cggcggcgcc gacggcatca ccgtgcacct gcgcgaggac
     1321 cggcgccata tccaggacga ggacgtgcgc cgcctgcgcg agctgaccca gatccggctc
     1381 aacctcgaga tggcggcgac cgcggagatg gttgacgtgg cgtgcagggt gaagccggaa
     1441 atggcgatgc tcgtgcccga gggccgccac gagatcacga ccgaaggcgg cctcgatatc
     1501 gtctctcagg agaaaagact gatcgcggtg gtcgcgcgcc tggcctccgc tggcatcgtg
     1561 gtgtcggtat tcatcgacgc cgaggtggcg caggtggagg cggcaaagcg catcggcgcg
     1621 gcggtgtgcg aggtgcatac cggcccgtac gcccacgctt tccattccag gggacgggac
     1681 gccgggagcc ctgtagtgat ttctgaattg aagaaaattc aaacggccgg cgagctgatc
     1741 cgctcgctcg gcatgcgctt caacgcagga cacgcgctca actacgtcaa cgttgagccg
     1801 gtggcgcgcc tgcaggggct gagagagctg catatcgggc acgccatcgt cagccgggcg
     1861 ctgttcgtcg gtatgcgcga ggcagtacgc gagatgaaga gcctcatggt ccaggctgcg
     1921 cggtgatcta cggcgtcggc accgacgtgg tcgagatcca gcgcatcgaa aaagccctgg
     1981 cccggttcgg cgagcgcttc gcgcagcgca tcctctgccg atcggaatta caaaggttcc
     2041 aaggccacag gcgaaaagcg agctatctcg ccaagcgctt cgccgccaag gaagcattca
     2101 gcaaagctct cggcaccggc atccacgcgc cggcgaactg gcacggggta tgggtgaaga
     2161 acctcgagtc cggcaaaccg gtcttcgatt tctccaggcc gctgaagaaa ctcctgaaaa
     2221 gcaaaggtgt gacccgcgcc catctctccc tttccgacga gcgcggcgta gcggtagcga
     2281 cggtgatcct ggagtgcgaa aagtaacact tccgccaggg ccggtagtgc tcgacgtcgc
     2341 tggcacggcg cttggcgacg cggaccgtcg gcggctcgcg catccggcga ccggtgcggt
     2401 aattttattt tcaagaaact atgagaaccc ggagcagctg aaagctctca cggaggaaat
     2461 agagcgcttg cgcgggcccg cgctgccggt gtgcgtcgac catgaa
//
LOCUS       DASPXW010000413         2662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_122917, whole genome shotgun sequence.
ACCESSION   DASPXW010000413 DASPXW010000000
VERSION     DASPXW010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122917"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="VEL04_09450"
     CDS             <1..565
                     /locus_tag="VEL04_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYT48636.1"
                     /translation="GYKVARIEEKTGQHASDTAQIVFDNCKTEHLLGQEGQGYRIALA
                     NLESGRINVAAQATGVARAAFEAALAYSRERKSMGKTLIQHQAVNFRLADMATGIEAG
                     RQLYLHAAALRDAGKPCIKEASMAKLFASEMAEVVCSAAMQIHGGYGYVADFPVERLW
                     RDVRVTQIYEGANDIQRLVIGRVLEEG"
     gene            562..2148
                     /locus_tag="VEL04_09455"
     CDS             562..2148
                     /locus_tag="VEL04_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013690312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HYT48637.1"
                     /translation="MSAIKSQISARSAEWRQNAARMQALVADLRAKVEAVSQGGDQAA
                     RDKHSARGKLLPRERMRTLLDAGSPFLEIGQLAAWGMYGGDVHSASIICGIGRVSGRE
                     CVIVANDATIKGGTYYPMTVKKHLRAQEIAAQNRLPCIYLVDSGGGYLPEQDNVFPDR
                     EHFGRIFYNIANMSAAGIPQIACVMGSCTAGGAYVPAMSDEAVIVKGQGTIFLGGPPL
                     VKAAIGEVVTPEELGGAEVHTRVSGVADHYALNDAHALSLVKKIVGNLNSRKSVSLDL
                     REPREPLYPAEELYGVIPADARKPYDIREILARLVDGSELDEFKQNYGTTLVTGFARL
                     HGYPVAILANNGILFSESSLKATHFIELACQRGIPLLFVQNITGYMVGKKYEAGGIAK
                     DGAKMVTAVATAKVPKFTLIVGGSFGAGNYGMCGRAYGPRFLWTWPNARISIMGGEQA
                     ASVLATVKGDFPSREAEEAFKAPIREQYERQGHPYYSSARLWDDGVVDPADTRRLLGL
                     AISASLNAPIEETKFGVFRM"
     gene            2163..2606
                     /locus_tag="VEL04_09460"
     CDS             2163..2606
                     /locus_tag="VEL04_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HYT48638.1"
                     /translation="MSEHRATVTWQRSGEFRYETYSRSHVLRFGAIEVPGSAARENIP
                     ATAAGAPGVDPEQAFIAAISACHMLWFLHLACQKKFVVERYLDEAVGVLDKTWISKVT
                     LQPAVTFSGTKPNEKEHAALHQRAHEKCFVANSVKSKVRVEPRIA"
BASE COUNT          501 a          855 c          883 g          423 t
ORIGIN      
        1 cggctacaag gtggcccgca tcgaagagaa gaccggccag cacgcgtcgg atacggcgca
       61 gatcgttttc gacaactgca agaccgagca cctcctcggc caggagggcc agggttaccg
      121 gattgcgctt gcgaacctcg agtcgggccg catcaacgtc gccgcccagg ccaccggcgt
      181 ggcgcgagcc gcctttgaag ccgcgcttgc atactcccgc gagcggaaaa gcatgggcaa
      241 gacgctgatc cagcaccagg cggtcaactt ccgcctcgcc gacatggcga ccgggatcga
      301 ggccggaagg cagttgtacc tgcacgcggc ggcgttgcgc gacgcgggca agccctgcat
      361 caaggaagcg tcgatggcga agctgttcgc ctccgagatg gccgaagtgg tctgctcggc
      421 agcgatgcag atccacggcg gttatggcta tgtcgccgat tttccggtcg agcggctgtg
      481 gcgcgatgtg cgggtgacgc agatctacga gggcgcgaac gacatccagc gcctcgtgat
      541 aggcagggtg ctggaagagg gatgagcgcc atcaaaagcc agatcagcgc gcggtcggcg
      601 gagtggcggc agaacgccgc gcgcatgcaa gccttggttg cggatctcag agcgaaagtc
      661 gaagccgtct cgcaaggcgg cgaccaggca gcgcgcgaca agcacagcgc gcgcggcaag
      721 ctgctgccgc gcgagcgcat gcgcacgctg ctcgatgcgg gctcgccgtt cctcgagatc
      781 ggccagcttg ccgcctgggg gatgtacggc ggcgacgtgc actcggcctc gatcatctgc
      841 ggcatcggcc gggtttcggg ccgggaatgc gtgatcgtgg cgaacgacgc caccatcaag
      901 ggcgggacct attacccgat gacggtgaaa aagcacctgc gcgcgcagga gattgcggcg
      961 cagaaccggc tgccgtgcat ctacctcgtc gattcggggg gcggctacct gcccgagcag
     1021 gacaacgtct ttcccgaccg cgagcacttc ggccgcatct tctacaacat cgccaacatg
     1081 tccgcggccg gcatcccgca gatcgcctgc gtgatgggct cttgcaccgc cggcggcgcg
     1141 tacgtgcccg ccatgtccga cgaggcggtc atcgtcaaag gccagggcac catctttctc
     1201 ggcgggccgc cgctggtgaa ggcggcgatc ggcgaagtgg tgacgcccga ggagctcggc
     1261 ggcgcggaag tgcatacgcg cgtctcgggc gtcgccgatc actatgcgct gaacgatgcg
     1321 catgccttgt ctctcgtaaa aaaaatagtc ggaaatttga acagcagaaa atccgtatcg
     1381 ctcgatctgc gcgaaccgcg agagcctctc taccctgccg aagagctgta cggcgtgatt
     1441 cccgccgatg cgcgcaagcc ctacgacatc cgcgagatcc tcgcgagact ggtggatggc
     1501 tccgagctcg acgagttcaa gcagaactac ggcaccacgc tggtcacggg cttcgcccgc
     1561 ctgcacggct acccggtggc gatcctcgcc aacaacggca ttctgttctc cgagtcctcg
     1621 ctcaaggcga ctcacttcat cgagctcgcc tgccagcgcg gcataccgct gctcttcgtg
     1681 cagaacatca ccggctacat ggtcggcaag aagtacgagg cgggcggcat cgccaaggac
     1741 ggggcgaaga tggtgactgc ggtggccact gcgaaagtgc ccaaattcac gctgatcgtc
     1801 ggcggctcgt tcggcgctgg caactacggc atgtgcggac gcgcctacgg cccccgcttc
     1861 ctgtggacgt ggccgaacgc gcgcatctcg atcatgggcg gcgagcaggc ggcgagcgtc
     1921 ctcgcgacgg tcaaggggga ttttccctcc agggaggctg aagaggcctt caaggcgccg
     1981 atccgcgagc agtacgagcg ccagggccat ccctattatt cgagcgcgcg gctctgggac
     2041 gacggtgtcg tcgatcccgc cgacacgcgg cggctgctgg gtctcgcgat ttccgcttcg
     2101 ctcaacgcgc cgatcgagga aacgaagttc ggcgtatttc ggatgtagcg cgcgaatgtg
     2161 agatgagcga gcaccgcgcg accgtgacct ggcagcgctc gggcgaattc cgctacgaga
     2221 cctattcgcg cagccacgtg ctgcgtttcg gagcgatcga ggtgccgggc agcgcggcgc
     2281 gggaaaacat ccccgccacg gctgccggcg cgccgggagt cgaccccgag caggccttca
     2341 tcgccgcgat ctcggcctgc cacatgctct ggttcctgca cctcgcctgc cagaagaaat
     2401 tcgtggtcga gcgctacctc gacgaggccg ttggcgtgct ggacaagacc tggatctcaa
     2461 aagtgactct gcaacccgca gtgaccttct cggggacaaa accaaacgag aaggagcacg
     2521 cggcgctgca tcagcgcgcg cacgagaagt gcttcgtcgc caactcggtg aaatccaagg
     2581 tccgggtcga gccgaggatc gcatgagcca tctcctcgtc gagaagaaag acgctgtcgc
     2641 gcgcgtcacg ctgaaccgcc cc
//
LOCUS       DASPXW010000414         3414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1632538, whole genome shotgun sequence.
ACCESSION   DASPXW010000414 DASPXW010000000
VERSION     DASPXW010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1632538"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VEL04_09465"
     CDS             complement(<1..245)
                     /locus_tag="VEL04_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008353626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYT48639.1"
                     /translation="MKTAFHKTATHAERILAAADKLFYRQGIRAVGVDAVAAEAGVSK
                     RTLYNHYPSKDDLIAAYLTAQFRHIAPSDAPAREQILG"
     gene            325..1560
                     /locus_tag="VEL04_09470"
     CDS             325..1560
                     /locus_tag="VEL04_09470"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYT48640.1"
                     /translation="MTPLQSRRAMLLLIALSGLIIALCMGLRQSLGLFMRPMTLDLGI
                     SAATFGFSIALQNIVLGVSQPFVGALADRYGPRPVLVVTALMYAAGLLLMVVSKSIPG
                     GLEVAGFLIGIGTAGTGFGVLIGTISRATPPEKRSQTVGIVAAAGSLGTMVLAPVGGW
                     LIDSFGWQAAMLAFAAIAGSMVLLSLPIREQPLSDKNSFSKKQNLGEALREAMGHRGY
                     LFMTLAFFACGFQLVFITTHLPAYLQLCGVAPGVAASALGLIGLFNAVGTYLFGLLGA
                     RYSQKHLLAMIYLLRTLFIVAFLLAPISAATTLVFAAAMGFLWLGVAPLVSGIIGRVF
                     GLTHFNTLYGIVFLSHQVGSFFGAWMGGVVFDRFGNYNFAWGALIAIGVTAFTLQWLM
                     DERPPRERRPAGEPAAVPA"
     gene            complement(1668..1898)
                     /locus_tag="VEL04_09475"
     CDS             complement(1668..1898)
                     /locus_tag="VEL04_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48641.1"
                     /translation="MKTTLELPDELLQRIKLRAVHRRRKLKDEIAHLLEAGMAQAPAP
                     EMPRKPPRPVRLRGRRPLTLREIEAAIAAGRD"
     gene            1990..2925
                     /locus_tag="VEL04_09480"
     CDS             1990..2925
                     /locus_tag="VEL04_09480"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HYT48642.1"
                     /translation="MRPELLALAARLSAQGERFALVTVVRREPPSSARVGDTALVTEG
                     GGYHGWAGGGCTRETVVREAQRAMAEGEPRFLSLSPDAAAESAPRARPGMVALPMTCD
                     SGGTVEIYVEPVLPVPRLFLFGASPAVRCLARIAHAMGYRVDVVHPDADQGDFPDAQG
                     VQKTFASETLPRGAHVLVATMGDDDLEVLRSVLAREPAYVGVIASRKRFETLRAELLQ
                     RGVPEAALVRVAAPAGLDLGARTPEEIALSVMAQIVQARRKPIEATPAEAVDPVCGMT
                     VAVAGARHTASVGGVVYYFCCAGCRSKFLQVKEDR"
     gene            2922..>3414
                     /locus_tag="VEL04_09485"
     CDS             2922..>3414
                     /locus_tag="VEL04_09485"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HYT48643.1"
                     /translation="MIPAPFDYHRAGTLDEAIGLLSRYKDQAKVLSGGMSLLPTLKLR
                     LGSFGHLVDISRIAGLEYIKEEKGAVRIGAGTRQCVLERSELIKSKYGVLADAVPLIA
                     DPLVRNRGTIGGNVANGDPANDEPAIVIALGAELVARGPNGERSIPANRFYKNLFVTD
                     LKTD"
BASE COUNT          482 a         1144 c         1187 g          601 t
ORIGIN      
        1 ccgaggatct gctcgcgcgc cggcgcatcc gagggcgcga tgtgcctgaa ttgcgccgtc
       61 agataggcgg cgatcaggtc gtccttggaa gggtagtggt tgtacagggt gcgcttgctg
      121 acgcccgcct cggcggcaac ggcgtcgaca ccgacggcgc ggatcccctg gcggtagaac
      181 agcttgtcgg cggcggcgag gatgcgctcg gcgtgggtgg cggttttgtg aaacgccgtc
      241 ttcatgtaca caggtcggtg taattaatgg ttgacatcag tataaggctt gcatgatgat
      301 ttagtacaca agcctgttta cttcatgacc cccctccaaa gccgccgcgc catgctgctc
      361 ctcatcgcgc tgagcggcct gatcatcgcg ctgtgcatgg gcctgcgcca gagcctgggc
      421 ctgttcatgc gcccgatgac gctcgacctg gggatctcgg cggcgacctt cgggttctcc
      481 atcgcgctgc agaacatcgt cctcggcgtg tcgcagcctt tcgtcggcgc gctcgccgac
      541 cgctacggac cgcggccggt gctggtcgtc accgcgctga tgtacgccgc cgggctgctg
      601 ctgatggtgg tctccaagag cattccgggc gggctggagg tggcgggttt tctcatcggc
      661 atcggcaccg ccggcaccgg cttcggcgtg ctgatcggca ccatctcgcg cgccacgccg
      721 cccgagaagc gcagccagac cgtcggcatc gtggccgccg caggctcgct cggcacgatg
      781 gtgctcgcgc cggtcggcgg ctggctgatc gacagcttcg gctggcaggc ggcgatgctg
      841 gccttcgcgg cgatcgcagg ctcgatggtg ctgctgtcgc tgccgatacg ggaacaaccg
      901 ctttccgata agaattcctt ttctaaaaag cagaatcttg gcgaggcgct gcgcgaggcg
      961 atgggccatc gcggctacct cttcatgacg ctcgcgttct tcgcctgcgg cttccagctg
     1021 gtgttcatca ccacgcacct gccggcgtac ctgcagctct gcggcgtggc gccgggcgtg
     1081 gcggcgagcg cgctcgggct tatcggcctg ttcaacgcgg tcggcaccta cctgttcggc
     1141 ctgctcggcg cgcgctacag ccagaagcac ctgctggcga tgatctacct gctgcgcacg
     1201 ctgttcatcg tcgccttcct gctggcgccg atcagcgccg ccaccaccct ggtgttcgcc
     1261 gcggcgatgg gcttcctgtg gttgggcgtg gcgccgctcg tcagcgggat catcggccgg
     1321 gtgttcggcc tgacgcactt caacacgctc tacggcatcg tgttcctcag ccaccaggtc
     1381 ggctcgttct tcggcgcctg gatgggcggc gtggtgttcg accgcttcgg caactacaac
     1441 ttcgcctggg gcgcgctgat cgcgatcggc gtcaccgcct tcacgctgca gtggctgatg
     1501 gacgagcgcc cgccgcgcga gcgcaggccc gccggcgagc cggccgcggt gccggcgtaa
     1561 cgcaagaacg cggttcgcgt cggtactgag gcaaggtcct gcgttcctga tatcagccgg
     1621 gcagagcctg cgcgagggac tgcgtcactg cgggcgccgc ccggcgccta gtcgcggccc
     1681 gcggcgatgg cggcttcgat ctcgcgcagg gtgagcgggc gccgcccgcg caggcgcacc
     1741 ggccggggcg gcttgcgcgg catctcgggc gcgggcgcct gcgccatgcc ggcctcgagc
     1801 agatgggcga tctcgtcctt cagcttgcgc ctgcggtgca cggcgcgcag cttgatgcgc
     1861 tgcaggagct cgtccggcag ttccagggtg gtcttcatcg gcccattttc ccataagcag
     1921 tgcagcatgc gggaattcgc gccgcgcgga aagtgtatgc gctaccatcg gcgggtttct
     1981 ggaggagcga tgcggcccga actgctggcg cttgccgcga ggttgtccgc acagggcgag
     2041 cgcttcgcgc tcgttacggt ggtgcggcgc gagccgccga gctcggcgcg cgtcggagat
     2101 acggcgctcg tcactgaggg cggcggctat cacggctggg cgggcggcgg ctgcacgcgc
     2161 gagacggtgg tgcgcgaggc gcagcgcgcc atggcggagg gcgagccgag gttcctgtct
     2221 ctttcccccg acgcggcggc cgaatctgcc cccagggcgc gccccggaat ggtcgcgctc
     2281 cccatgacct gcgacagcgg aggcaccgtg gagatctacg tcgagcccgt gctgcccgtt
     2341 ccgcgcctgt ttctcttcgg cgcctcgccg gcggtgcgct gcctcgcgcg catcgcccac
     2401 gcgatgggct atcgggtgga cgtcgtccac ccggacgcgg accagggtga ttttcccgac
     2461 gcgcagggcg tgcagaagac ttttgcctcg gaaaccctgc ccaggggcgc gcacgtgctg
     2521 gtcgcgacca tgggcgacga cgacctcgag gtgctgcgct cggtgctcgc gcgcgagccg
     2581 gcctacgtgg gcgtcatcgc cagccgcaag cgtttcgaga cgctgcgcgc cgagctgctg
     2641 cagcgcggag tgcccgaagc cgcgctcgtc cgcgtggcgg cgcccgcggg cctggacctc
     2701 ggcgcccgca cgccggaaga gatcgccctc agcgtgatgg cgcaaatcgt ccaggcgaga
     2761 agaaaaccga ttgaagcaac gccggccgag gcggtggacc ccgtctgcgg catgaccgtt
     2821 gccgtcgcag gcgcgcgcca cacggccagt gtcggcggtg tcgtctacta cttctgctgc
     2881 gccggctgcc gaagcaaatt cctccaagtt aaggaagacc gatgatccct gcgcctttcg
     2941 actaccaccg ggccggcacg ctggacgaag cgatcggcct gctttcgcgc tacaaggacc
     3001 aggcgaaagt cctctcgggc ggcatgagcc tgctgccgac gcttaagctg cggctcggct
     3061 cgttcggcca cctggtcgac atcagccgca tcgcgggcct cgagtacatc aaggaagaga
     3121 agggcgcggt gcgcatcggc gccggcacgc gccagtgcgt gctcgagcgc tccgagctga
     3181 tcaaatccaa atacggcgtg cttgccgatg ccgtgccatt gatcgccgac cctctggtgc
     3241 gcaaccgcgg cacgatcggc ggcaacgtcg ccaacggcga cccggcgaac gacgagcccg
     3301 ccatcgtcat cgcgctcggc gcggagctgg tggcgcgcgg gccgaatggc gagcgcagca
     3361 tcccggcgaa tcggttttac aaaaatcttt ttgttacaga tcttaaaacc gatg
//
LOCUS       DASPXW010000415         2697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_625794, whole genome shotgun sequence.
ACCESSION   DASPXW010000415 DASPXW010000000
VERSION     DASPXW010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625794"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VEL04_09490"
     CDS             <1..523
                     /locus_tag="VEL04_09490"
                     /inference="COORDINATES: protein motif:HMM:NF042738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6701 domain-containing protein"
                     /protein_id="HYT48644.1"
                     /translation="AGSEFRYGIVRLQDVFAPLAGNASGNAPVTILAQYWTGTAFATN
                     TLDNCTTFTEKNFVLYSPQGAVTSANVPTPNGGNGNVSGNGTLVSGVAQVNVKKPSPA
                     ISTPGSVRICFDLDSSTTQRDPACVAVTKANQAHLQGAWAAGSYDKDPNATVGFGIYG
                     SQPRNFIFFRENY"
     gene            complement(548..1723)
                     /locus_tag="VEL04_09495"
     CDS             complement(548..1723)
                     /locus_tag="VEL04_09495"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYT48645.1"
                     /translation="MSLINKVLRDLDRRHAISTGDATVPPAQVRAVEPRSDGHEWFWR
                     VVAVLTLAALTWVSWVIYQVRPRPLATELALQAAEEARARPKPVRPVQVVEVAPKPPV
                     VPAESAPPAAEQAKPPAEPVAQAVTAPKPAPAPELLRLASSIETPIVEPARKSPEKAP
                     AVAAEKPAARVATPKPPAAKTAKAPLFLAGKSRVERRDRALSPSESADTEFRRAVELL
                     KRGRGAEAEQAFAAALEFDPRHRGARQALVALNFERGQLENARRLLQDGLAADPAQPD
                     FAVALARIYIERGNLQAALAAMQGSEAAAANHAEYHVLRGTILQRLGRHAEAADAYRT
                     ALNVQSSMPHAWMGLGISLEALQQRAEAAQAFRQALAAGPVSAELKTFAEQRIRALR"
     gene            complement(1733..2605)
                     /locus_tag="VEL04_09500"
     CDS             complement(1733..2605)
                     /locus_tag="VEL04_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYT48646.1"
                     /translation="MNYLAHFGLREPPFGITPDTSFFFPCRSSQQALNTLLIALDNGE
                     GFIKITGEVGTGKTMLCRKFLATLDETAWVSAYIPNPNLEPRALLFAFAEELGVSLDP
                     GLDQYRLLKGLTRSLLDLARTRRRVVLCMDESQAMPLDTLEALRLLTNLETEKRKLLQ
                     VVLFGQPELDRKLASESVRQLRQRITFQHDLQTLDEDEVGAYFTHRLRVAGYAGPALL
                     TAGARRSLYQASRGVPRLVNILSHKTLLLLYGEDGARIERRHVHAAAVDTPAAVQPRR
                     WWSLPRLRLSRAEK"
BASE COUNT          407 a          893 c          960 g          437 t
ORIGIN      
        1 cgcgggcagt gaattccgct atggcatcgt ccgattgcag gacgtgttcg cgccgctggc
       61 cggcaacgcg agcggcaacg cgccggtgac gatcctcgcc cagtactgga ccgggacggc
      121 gttcgcgacc aacacgctag acaactgcac cacgttcacc gagaagaatt tcgtgctcta
      181 cagcccccag ggcgcggtta cgtcggcgaa cgtgcccacg ccgaacggcg gaaatggcaa
      241 tgtttccggc aacggcacgc tcgtttccgg cgtcgcccag gttaacgtca agaaaccgag
      301 cccggctatc tcgacgccgg gcagcgtgcg tatctgcttc gatctcgact cctcgacgac
      361 gcagcgggac ccggcgtgtg tcgccgtgac caaggctaat caagcccatt tgcaaggggc
      421 ctgggcggcc ggcagttacg acaaggatcc gaacgccacg gtcgggttcg gcatttacgg
      481 gtcgcagccc agaaacttca ttttcttccg cgagaactac tgaggcttcg cgcggaacgc
      541 tgaggcgtta acgcagcgcg cggatgcgct gctcggcgaa ggtcttgagc tccgcgctga
      601 ccgggccggc ggcgagcgct tggcggaagg cctgcgcggc ctcggcgcgc tgctggaggg
      661 cctcgagcga gatgccgagc cccatccagg cgtgcggcat cgacgactgt acgttaagcg
      721 cggtgcggta ggcatcggcg gcctcagcgt ggcgccccag gcgctgaagg atggtgccgc
      781 gcaaaacgtg gtactccgcg tggttcgctg ccgcagcttc ggaaccctgc atcgcggcga
      841 gcgctgcctg caggttgccg cgctcgatgt agatgcgcgc cagcgcgacg gcgaagtcgg
      901 gctgcgcagg atcggcggcc aggccgtcct gcagcagcct gcgggcattt tccagctggc
      961 cgcgctcgaa gttgagcgcg accagcgcct ggcgggcgcc gcggtgcctg ggatcgaact
     1021 cgagcgccgc cgcgaacgct tgctcggcct cggcgccgcg cccgcgcttg agcagctcga
     1081 cggcgcggcg aaattccgtg tcggcgcttt cggaggggct gagcgcgcgg tcgcgccgct
     1141 cgacgcggga tttccccgcc agaaagagcg gcgccttagc tgtcttggcg gcaggcggct
     1201 tcggggtcgc gacgcgcgcc gccggtttct cggccgcgac tgccggggct ttctccgggg
     1261 atttacgcgc cggctcgacg atcggcgtct cgatggaaga cgcgaggcgc agcagctcgg
     1321 gtgcgggggc tggcttgggc gcggtcaccg cctgcgctac aggctctgcc ggcggcttgg
     1381 cctgctcggc ggccggcggt gcggattcgg ccggaacgac tggcggcttc ggcgccacct
     1441 ccaccacctg taccggccgc actggcttgg ggcgcgcgcg cgcttcctcg gccgcctgga
     1501 gcgcgagctc ggtggcaagc ggccgcggcc gcacctggta gatgacccag gacacccagg
     1561 tgagtgcggc cagcgtcaga accgcgacca cgcgccagaa ccactcgtgc ccgtcgctgc
     1621 gcggctcgac ggcgcgcacc tgcgccgggg gaacggtcgc gtcgccggtc gagatcgcgt
     1681 gacgccgatc gagatcgcgg agcaccttgt tgatcaggct catgcgcggg cgctattttt
     1741 ccgcacggct cagcctcagg cgcggcaacg accaccagcg gcgcggctgc acggccgcgg
     1801 gcgtgtcgac tgcggcggcg tgcacgtggc gccgctcgat gcgagcgccg tcttctccgt
     1861 acaggagaag cagcgtcttg tgcgacagga tgttgacgag gcgcggcacg ccgcggcttg
     1921 cctggtacag gctgcggcgc gcgcccgctg taaggagcgc cggtcccgca tatcccgcga
     1981 cccgcagccg gtgcgtgaaa taggcgccga cttcgtcctc gtcgagtgtc tgcaagtcat
     2041 gctggaaagt gatgcgctgg cgcagttgcc gcaccgattc ggaggccagc ttgcggtcga
     2101 gctcgggctg gccgaacagc accacctgca gcagcttgcg tttctcggtc tcgagattgg
     2161 tgagcaggcg caacgcttcc agcgtgtcga gcggcattgc ctggctctcg tccatgcaca
     2221 gcaccaccct gcggcgggtc cgcgccaggt cgagcagcga ccgcgtcagg cccttcagca
     2281 ggcggtattg gtcgagtccc gggtcgaggc tgactcccag ctcctcggca aacgcgaaca
     2341 gcagcgcgcg cggctcgagg ttgggattgg ggatgtaggc cgagacccac gccgtctcgt
     2401 cgagcgtagc gaggaacttg cggcacagca tggtcttgcc ggtgccgact tcgccggtga
     2461 tcttgatgaa gccctcgccg ttgtcgagcg cgatcaacag ggtgttgagg gcctgctggc
     2521 tgctgcggca gggaaagaaa aagctggtgt ccggcgtaat gccgaaaggc ggctcgcgca
     2581 ggccgaaatg cgcgaggtag ttcactatct gcgctccgcg gcgggcggcc gctgatacat
     2641 gtcgcgcacc cgttcctgag tatcgcgcaa gtcctgctgc caatcccggt catcacc
//
LOCUS       DASPXW010000416         2760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1259614, whole genome shotgun sequence.
ACCESSION   DASPXW010000416 DASPXW010000000
VERSION     DASPXW010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1259614"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1226
                     /locus_tag="VEL04_09505"
     CDS             <1..1226
                     /locus_tag="VEL04_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HYT48647.1"
                     /translation="GDAYIDFGALGAGVGAEEAPVVKMLQSIFEDAIQVNASDVHIEP
                     LENRLQIRFRIDGALMPQTQSDPKIAPALALRLKLMAGLDISEKRLPQDGRLMVAVRD
                     QKVDVRMSTMPCQYGETVVLRLLNRKATLQSLDRLGMPEDIRGRFGEIIRRTAGMVLV
                     TGPTGSGKTTTLYAALAELNTADRKIITVEDPVEYRLAGITQVQVNEKIDLSFARVLR
                     STLRQDPDVILVGEIRDEETAQIGLRAALTGHLVFSTLHTKDAASTPLRLIDMGAPSY
                     MVATSVHAVIAQRLVRLICESCATDAEPDAQEARWLEAVSGPRTGERRFRRGQGCSHC
                     NGIGYAGRSGVYEMLEMTPDLVYAANTADPNAFLEAARPHLRGRTLIDRALALVFAGR
                     TTVAEAMKVAVQLEE"
     gene            1226..2455
                     /locus_tag="VEL04_09510"
     CDS             1226..2455
                     /locus_tag="VEL04_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HYT48648.1"
                     /translation="MPVFAYRGRGARGELVTGTLEAPDQGAVADQLLNTGVTPVDIKV
                     TSGTAESGSPGWLARLLAPKVTLVDTMLFSRQMYTLLKAGVPILRALAGLQESAINPS
                     LRRVIADLRVSLDAGRELSTAMRGHLEVFSEFYISVVRVGETTGQLEEAFNRLFGYLE
                     FEREVRDRIKTALRYPTIVILVIAAAIAIVNFAVIPAFAKIFEANKVALPLLTRVLIG
                     TSDLFVAFWPLLLAGIVIAIVGFMAYTGTEAGRYNWDRIKLRIPVAGKILFKATLSRF
                     ARAFAIAARAGVPIVQGLTVVASVVDNSYISRRVEQMREGVERGESVLRTAVTAGVFT
                     PVVLQMIAVGEETGELDDLLNEVADMYEREVDYEIRNLAVNVEPIITIVLGVLVLILA
                     LGVFLPIWELGKVMLKK"
     gene            2455..>2760
                     /locus_tag="VEL04_09515"
     CDS             2455..>2760
                     /locus_tag="VEL04_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48649.1"
                     /translation="MLPLKIYGRRPLTRLEAGLYGGMAAILIAIFARYLLDFMETAER
                     IRMELTLSHVISGVNTRVAYDMLQGKTPADLAATRTDPFELARMAPTNFLGSGDSSNI
                     "
BASE COUNT          472 a          915 c          929 g          444 t
ORIGIN      
        1 tcggcgatgc gtacatcgac ttcggcgcgc tcggcgccgg cgtcggcgcc gaggaggcgc
       61 cggtggtgaa gatgctgcag tcgatcttcg aggacgcgat ccaggtgaat gcctccgacg
      121 tgcacatcga gccgctggaa aaccgcctgc agatccgctt tcgcatcgac ggcgcgctga
      181 tgccgcagac ccagagcgat ccaaagatcg ccccggcgct cgccctgcgg ctcaagctca
      241 tggcggggct cgacatctcg gaaaagcgcc tgccgcagga cggccgcctg atggtcgcgg
      301 tgcgcgacca gaaagtcgac gtgcgcatgt cgaccatgcc ctgtcagtac ggcgagacgg
      361 tggtgctgcg gctgctcaac cgcaaggcga cgttgcagtc gctggaccgg cttggcatgc
      421 cggaggacat ccgcggccgc ttcggcgaaa tcatccggcg caccgccggc atggtgctgg
      481 tcaccgggcc gaccggcagc ggcaagacga cgacgctcta tgccgcgctg gcggagctca
      541 acaccgccga ccgcaagatc atcaccgtcg aggacccggt cgaataccgg ctggccggca
      601 tcacccaggt ccaggtcaac gagaagatcg acctgagctt cgcgcgcgtg ctgcgctcga
      661 cgctgcgcca ggaccccgac gtgatcctgg tcggcgagat ccgcgacgag gaaaccgcgc
      721 agatcgggtt gcgcgcggcg ctcaccggcc acctggtgtt ctcgacgctg cacaccaagg
      781 acgccgcgag cacgccgctg cggctgatcg acatgggcgc gccgagctac atggtggcga
      841 cctcggtgca cgcggtgata gcgcagcgcc tcgtgcgcct gatctgcgag agctgcgcga
      901 ccgacgccga gcccgatgcg caggaagcgc gctggctgga ggcggtgagc gggccgcgca
      961 caggcgagcg gcgtttccgc cgcggccagg gctgctcgca ctgcaacggc atcggctacg
     1021 ccggccgcag cggcgtctac gaaatgctcg aaatgacgcc cgatctggtg tacgcggcca
     1081 acaccgccga cccgaacgcg ttcctcgaag ccgcgcgccc gcacctgcgc gggcggacgc
     1141 ttatcgaccg cgcgctcgca ctggtgttcg ccggccgcac cacggtcgcc gaggcgatga
     1201 aggtggcggt acagctcgag gaataatgcc ggttttcgcc taccgcggcc ggggagcccg
     1261 aggcgagctg gtgaccggca cgctggaagc tcccgaccag ggcgcggtcg ccgaccagct
     1321 gctcaacacc ggtgtgacgc cggtcgacat caaagtcact tcgggcaccg cagaatccgg
     1381 ctcgcccggc tggctggcgc gcctgctcgc gccgaaggtg acgctggtcg acacgatgct
     1441 gttctcgcgc cagatgtaca ccctgctgaa ggccggcgtg ccgatcctgc gcgcgctggc
     1501 aggattgcag gaatcggcaa tcaacccgag cctgcgccgc gtcatcgccg acctgcgcgt
     1561 gagcctggac gcggggcgcg agctctcgac cgcgatgcgc ggccacctcg aggtgttctc
     1621 ggagttctac atcagcgtgg tgcgcgtggg ggagacgacc ggacagctcg aagaggcatt
     1681 caaccgcctg ttcgggtatc tcgagttcga gcgcgaggtg cgcgaccgga tcaagaccgc
     1741 gctgcgctat ccgacgatcg tgatcctggt gatcgcggcg gcgatcgcga tcgtcaattt
     1801 cgcggtgatc cccgctttcg ccaagatctt cgaagcgaac aaggtggcgc tgccgctgct
     1861 gacgcgcgtc ctgatcggca cctcggatct gttcgtcgcg ttctggccgc tgctgctcgc
     1921 cggcatcgtg atcgcgatcg tcggattcat ggcctatacg gggaccgaag cgggccgcta
     1981 caactgggat cgcatcaagc tgcgcattcc ggtcgccggg aaaattcttt tcaaggccac
     2041 cctctcgcgc ttcgctcgcg cctttgcgat cgccgcccgc gccggggtgc ccatcgtgca
     2101 gggtctcacc gtggtggcgt cggtcgtcga caacagctac atctcgcgcc gcgtcgagca
     2161 gatgcgcgaa ggcgtcgagc gcggcgaaag cgtgctgcgg accgcggtga ccgccggcgt
     2221 cttcactccg gtagtcctgc agatgatcgc cgtcggcgag gagaccggcg agctcgacga
     2281 cctgctgaac gaagttgccg acatgtacga gcgcgaagtg gattacgaga tcaggaacct
     2341 tgccgtgaac gtcgagccga tcatcaccat cgtgctcggc gtactggtgc tgatcctcgc
     2401 cctcggcgtg ttcctgccga tctgggagct gggcaaggtg atgctgaaga agtgatgctg
     2461 ccgctcaaaa tttacggccg gcgccccctg acgaggctgg aagcggggct ctatggcggc
     2521 atggcggcaa tcctgatcgc gatattcgcg cgctacttgc tcgacttcat ggaaaccgcg
     2581 gaacgcatca ggatggaact cacgctcagc cacgtcatct ctggcgtcaa tacgagagtc
     2641 gcctacgaca tgctgcaagg gaaaacgccc gcagacctcg cggcaacccg caccgatccc
     2701 ttcgagctgg cgaggatggc gcccacgaat tttctgggga gcggcgattc gtcgaacatc
//
LOCUS       DASPXW010000417         3440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1131466, whole genome shotgun sequence.
ACCESSION   DASPXW010000417 DASPXW010000000
VERSION     DASPXW010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131466"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="VEL04_09520"
     CDS             complement(<1..1122)
                     /locus_tag="VEL04_09520"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HYT48650.1"
                     /translation="MLSKPVYVVDGARSPFLKSKNRPGPFAASDLATQAGRALLMRQS
                     FEPPELDEVILGCAAPSVDEVNIGRVAALRMGCGEKVPGWTVMRNCASGMQALDSGIN
                     NILAGRSSLVLAGGVDALSRAPLLYGEKMVLWFSDMAAARSTGQRLAMFAKLPLKALL
                     APVIGIMKGLTDPMVGLLMGQTAENLAYRFGVTRAQMDAFAVASHQKVARAQDEGRLK
                     PGGGELEALYDPKGNAYALDDGVRKDASIENLKKLKPFFDRKYGNVTAGNSSQITDGA
                     AWLVLAGEDALRKFDLKPIGRIVDSQWAGLDPAQMGLGPVHAATPILKRNGLGLNDLD
                     FWEINEAFAAQVLGCLAAWRDEKYCKEELGLDSALGALDE"
     gene            complement(1122..>3440)
                     /locus_tag="VEL04_09525"
     CDS             complement(1122..>3440)
                     /locus_tag="VEL04_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HYT48651.1"
                     /translation="VFAALAALLNIPFVRRKAFTDHVLALYRRILPDMSSTEKEAIDA
                     GTVWWDADLFSGRPDWNKLLAIPVPRLSAEEQAFLDGPVEELCAMCNDWEITHERQDL
                     PPQVWQFIKDRGFLGMIIPKRYGGLGFSALAHSAVVMKLSTRSGTAAISVMVPNSLGP
                     AELLLHFGSEEQKNHYLPRLAKGLEVPCFALTSPEAGSDAASIPDYGIVCKGTWQGKE
                     MLGMRVTWDKRYITLGPVATLLGLAFRLYDPEKLISEKEDLGITCALVPTHTPGVNIG
                     RRHLPLNAVFQNGPNSGKDVFMPLDWIIGGPEYAGKGWMMLMTCLAAGRAISLPTSSV
                     GGVKALTRFTGAYARVRSQFKTPIGRLEGVEEALGRIAAHCYMMDATRIMTAGAVDLG
                     EKPAVLSAIAKYHITERARLCVNDAMDIHGGKGICLGPNNWVGRGYQMTPIAITVEGA
                     NILTRTLIIFGQGAIRAHPYVLREMRAAKEMAGAPASREFDDAITSHIGHVISNGVRA
                     FVHGITSSRLAHTPRNAAPETMHYYRFTSRLSAAFAFLADMSMLAMGGALKRKEKISG
                     RLGDVLSMLYLVSATLKHYEDQGRIREDLPLIRWGVRDALYRAQEAIDGILSNFPVKS
                     LATLLRWTLFPLGMSFRPPLDSRNHESAKIALEPGAARDRLTAGMYVPRGDDATGTLE
                     AAFVAAIACEPIDEKLRKAVKKGKIVPRPGVDLGSLAKENNLITAEEYAQWSRKEALR
                     KSVIKVDDFPQDFGRAEAAEKLAEKPLSARAA"
BASE COUNT          544 a         1142 c         1145 g          609 t
ORIGIN      
        1 ttcgtcgagc gcgccgagcg cgctgtccag gccgagctcc tccttgcagt acttctcgtc
       61 ccgccacgcc gcgaggcagc ccagcacctg cgcggcgaac gcctcgttga tttcccagaa
      121 gtcgaggtcg ttcaggccga ggccgttgcg tttgaggatc ggcgtcgccg cgtgcaccgg
      181 gccgagcccc atctgcgccg ggtcgagccc cgcccattgg gaatccacga tgcggccaat
      241 gggtttcaaa tcaaatttcc ttaaagcatc ttcgccggcg aggaccagcc atgccgcgcc
      301 atcggtgatc tgcgagctgt tgcccgcggt gacgttgcca tatttgcggt cgaaaaatgg
      361 tttcaatttt ttcagatttt caattgaagc atccttacgc acgccgtcgt ccagcgcata
      421 agcattgccc ttcgggtcgt agagcgcttc gagctcgccg ccgccgggct tcaagcgtcc
      481 ttcgtcctgc gccctcgcca ctttctggtg cgaggccacg gcaaaggcgt ccatctgcgc
      541 gcgcgtgacg ccgaagcggt acgcgagatt ctcggcggtc tgacccatca gcagcccgac
      601 catcgggtcg gtgagcccct tcatgatgcc gatcaccggc gctagcagcg ctttcagagg
      661 cagcttcgcg aacatggcaa gccgttgtcc ggttgaacgg gcggctgcca tgtcggaaaa
      721 ccacagcacc atcttctcgc catagaggag cggcgcgcgc gacagcgcgt cgacgccgcc
      781 ggcgagcacc aggctcgagc ggccggcgag gatgttgttg atgcccgaat cgagcgcctg
      841 catgcccgag gcgcagttgc gcatcacggt ccagccgggg actttctcgc cgcagcccat
      901 gcgcagcgcg gcgacccggc cgatgttcac ctcgtcgacc gagggcgcgg cgcagccgag
      961 gatcacctcg tcgagctccg gcggctcgaa gctctggcgc atgaggagcg cgcgcccggc
     1021 ctgggtcgcg aggtcgctcg ccgcgaacgg ccccgggcgg ttcttcgatt tcaggaacgg
     1081 cgagcgcgcg ccgtcgacga cgtagaccgg tttcgagagc atcacgcagc cctcgcggaa
     1141 agcggcttct ccgccagttt ctcagcggcc tctgctctgc cgaaatcctg cgggaagtcg
     1201 tcgaccttga tcacgctctt gcgcagcgcc tccttgcggc tccactgcgc gtactcctca
     1261 gccgttatca gattgttttc ctttgcgaga gagcctagat ccacccctgg tcgtggcacg
     1321 atcttgccct tcttcaccgc cttgcgcagc ttctcgtcga tcggctcgca ggcgatcgcg
     1381 gcgacgaacg ccgcttcgag cgtgccggtg gcgtcgtcgc ccctgggcac gtacatgccg
     1441 gcggtgagcc ggtcgcgcgc cgcgccgggc tcgagcgcga tcttcgccga ctcgtggttg
     1501 cgcgaatcaa gcggcgggcg gaacgacatt cccaatggga agagggtcca gcgcagcagc
     1561 gtcgcgagcg acttgaccgg gaaattcgac agaatgccgt cgatcgcctc ctgcgcgcgg
     1621 tacagcgcgt cgcgcacccc ccagcggatc agcggcaggt cctcgcggat gcggccctgg
     1681 tcctcgtagt gcttgagcgt cgcggagacg aggtacagca tcgacagcac gtcgcccagg
     1741 cgcccggaga tcttctcctt gcgcttcagc gccccgccca tcgccagcat cgacatgtcg
     1801 gcgaggaacg cgaatgccgc cgagaggcgc gacgtgaacc ggtagtaatg catcgtttcg
     1861 ggcgctgcgt tgcgcggcgt gtgggcgagg cgcgagctgg tgatgccgtg cacgaaggcg
     1921 cgcacgccgt tcgagatcac gtggccgatg tgcgaggtga tggcgtcgtc gaactcccgc
     1981 gaagctggcg ccccagccat ttccttcgcg gcgcgcatct cgcgcagcac gtacggatga
     2041 gcgcggatcg cgccctggcc gaagatgatg agggtgcgcg tcaggatgtt ggcgccctcg
     2101 accgtgatcg cgatcggcgt catctggtag ccgcgcccga cccagttgtt cggcccgagg
     2161 cagatgccct tgccgccgtg gatgtccatg gcgtcgttga cgcacaggcg cgcgcgctcg
     2221 gtaatgtggt acttggcgat cgccgagagc acggcgggct tttcgccgag gtcgaccgcg
     2281 ccggcggtca tgatgcgcgt cgcgtccatc atgtagcagt gcgcggcgat gcgcccgagg
     2341 gcttcctcga cgccttcgag cctgccgatc ggcgtcttga actggctgcg cacgcgcgca
     2401 taggcgccgg tgaagcgcgt cagcgccttc accccgccga ccgaggaggt cggcagcgag
     2461 atcgcgcgtc ccgccgcgag gcaggtcatc agcatcatcc agcctttgcc ggcgtattcc
     2521 ggcccgccga tgatccagtc gagcggcatg aacacgtcct tgcccgagtt cggcccgttc
     2581 tggaacaccg cgttgagcgg caggtgcctg cggccgatgt tgacgcccgg cgtgtgggtc
     2641 ggcaccaggg cgcaagtgat gcccagatct tctttttcag agatcagttt ttcaggatca
     2701 tagagccgga aagcgagacc gagcagcgtg gcgaccgggc cgagcgtgat gtagcgcttg
     2761 tcccaggtga cgcgcatgcc gagcatctcc ttgccctgcc acgtcccctt gcacacgatg
     2821 ccgtagtcgg ggatcgaggc ggcgtcggag ccggcctcgg gactggtgag cgcgaagcag
     2881 ggaacttcga ggcccttcgc gaggcgcggc aggtaatggt tcttctgctc ctcgctgccg
     2941 aagtgcagca ggagctcggc aggcccgagc gagttcggca ccatcaccga gatcgccgcg
     3001 gttcccgagc gcgtcgagag cttcatgacc actgccgagt gcgccagcgc cgagaagccg
     3061 aggccgccgt agcgcttcgg gatgatcatg ccgaggaagc ccctgtcctt gatgaactgc
     3121 cagacctgcg gcggcaggtc ctggcgctcg tgggtgatct cccagtcgtt gcacattgcg
     3181 cacagctctt cgaccgggcc gtcgaggaac gcctgctcct cggcggacag ccgcggcacc
     3241 ggtatggcga gcagcttgtt ccagtcgggc ctgccggaga acaggtccgc gtcccaccac
     3301 acggtgccgg catcgatcgc ttccttctcg gtcgacgaca tgtcggggag gatgcggcga
     3361 tacagggcga gcacgtgatc ggtgaaagcc ttcctcctga cgaaagggat gttcaggagc
     3421 gcggcgagcg ccgcgaagac
//
LOCUS       DASPXW010000418         3158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_627549, whole genome shotgun sequence.
ACCESSION   DASPXW010000418 DASPXW010000000
VERSION     DASPXW010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627549"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="VEL04_09530"
     CDS             complement(<1..409)
                     /locus_tag="VEL04_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein"
                     /protein_id="HYT48652.1"
                     /translation="MDYDLIVVGAGPVGASLARASRDLSVALVGHERRAPQSTDGGAF
                     DARVYALSPGNVAFLRGLGAWQRIPAARVQPVHAMRVFGDDGTSCIEFDAYRAGVPEL
                     AWIVEDKALQDALWSDLDVDVFAPAVCESLGVDE"
     gene            442..518
                     /locus_tag="VEL04_09535"
     tRNA            442..518
                     /locus_tag="VEL04_09535"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:476..478,aa:Met,seq:cat)
     gene            534..833
                     /locus_tag="VEL04_09540"
     CDS             534..833
                     /locus_tag="VEL04_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48653.1"
                     /translation="MRIFLAIAAFAGFCAVAGAQVRTIPEDAKRAQMRHVQDMLIELD
                     GAPQRLAPGAQIRDASNRVILPTAIPEGVQVKYRLDAAGQVRQVWILTPEEAAKP"
     gene            833..2149
                     /gene="miaB"
                     /locus_tag="VEL04_09545"
     CDS             833..2149
                     /gene="miaB"
                     /locus_tag="VEL04_09545"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HYT48654.1"
                     /translation="MGKLFLRTFGCQMNQYDSEKIAGVLRAAEGLEAAARPEDADVIV
                     FNTCSVREKAQEKVFADLGRVKHLKRANPALMIAVGGCVASQEGAGLVERAPYVDVVF
                     GPQTLHRLPELLARRRASGKPQVDVSFPEIEKFDHLPPARVAGAAAFVSIMEGCSKYC
                     SFCVVPYTRGEEVSRPFDDVVAEIAGLAARGVKEVTLLGQNVNAWRGEIAREPADFAE
                     LLYFICEIEGVERMRYTTSHPREFTQRLVDAHAAIAKLAPHVHLPVQSGADRVLAAMK
                     RGYTSLEYRSIARRLKRARPGLSLSSDFIVGFPGETEADFAATLKLASEIGFDGSFSF
                     LYSPRPGTPAAELPDDTPHEEKLSRLQRLQAQLDSQARAISESMVGTTQCVLVEKPSK
                     KNPNELCGRTGNNRVVNFPGAEGLINTFADVKITCALSHSLRGELT"
     gene            2162..3106
                     /locus_tag="VEL04_09550"
     CDS             2162..3106
                     /locus_tag="VEL04_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HYT48655.1"
                     /translation="MRAKPVEVRLDPVDNQRLARLCGALDENLRQIESALDVTIARRG
                     AKFTVRGHQAQHAAQALEHFYDRASGDLTLDDVQLGLTELLQGNQPALAEGPQLLTRR
                     QDLHGRTPHQIEYIEHILAHDLTLGIGPAGTGKTYLAVACGVDALERDKVKRIVLVRP
                     AVEAGERLGFLPGDLAQKVDPYLRPLYDALYDLMGFEKTGKLLERGTIELAPLAYMRG
                     RTLNHAFVILDEAQNTTPEQMKMFLTRIGFGAKAVVNGDVTQIDLARGQKSGLIEARR
                     ILEGVRGIAFTQFGAQDVVRHPLVARIIDAYEANPAQE"
BASE COUNT          541 a         1074 c         1047 g          496 t
ORIGIN      
        1 cctcatcgac cccgaggctt tcgcacacgg cgggcgcgaa cacgtcgaca tcgaggtcgc
       61 tccagagcgc atcctgcagc gccttgtcct cgacgatcca tgccagctcg ggcacgccgg
      121 cgcggtaggc gtcgaactcg atgcacgagg tgccgtcgtc gccgaacacg cgcatcgcgt
      181 gcaccggctg cacgcgtgcg gcgggaatgc gctgccaggc gccgaggccg cgcaggaacg
      241 cgacgttgcc ggggctgagc gcgtacaccc gggcatcgaa tgccccgccg tcggtcgatt
      301 gcggcgcgcg gcgctcgtgg ccgacgagcg ccaccgaaag atcgcgcgac gcgcgcgcga
      361 gacttgcgcc caccgggcct gcgcccacta cgatgaggtc gtagtccacc cggtttataa
      421 ttgcggcatc gatcgtgtcg tggcgaatta gctcagctgg ttagagcagc ggaatcataa
      481 tccgtgtgtc cggggttcga gtccctgatt cgccaccatt tccctctcgg cgcatgagaa
      541 tttttcttgc catcgctgct ttcgccgggt tttgcgccgt agcgggcgcg caggtgcgca
      601 ccatcccgga ggatgcgaag cgcgcgcaga tgcgccacgt ccaggacatg ctgatcgagc
      661 tcgatggcgc accgcagcgc ctcgcgcccg gggcgcagat ccgtgacgcc tcgaaccggg
      721 tgatcctgcc gaccgccatc cccgagggcg tgcaggtgaa ataccggctc gatgccgcgg
      781 gccaggtgcg ccaggtgtgg atcctgactc ccgaagaggc ggcgaagccg taatgggcaa
      841 gctctttctg cgtaccttcg gctgccagat gaaccagtac gactcggaga agatcgccgg
      901 cgtgctgcgc gcggccgagg gactggaagc cgccgcgcgt cccgaggacg ccgacgtcat
      961 cgtgttcaac acctgctcgg tgcgcgagaa ggcgcaggag aaggtgttcg ccgatctcgg
     1021 gcgggtcaag cacctgaagc gcgccaaccc cgccctgatg atcgcggtcg gcggctgcgt
     1081 cgcgagccag gaaggcgcag gcctcgtcga gcgcgcgccc tacgtcgacg tggtgttcgg
     1141 cccgcagacg ctgcaccggc tgccggagct gctcgcgcgc aggcgcgcaa gcggcaagcc
     1201 gcaggtcgac gtgagcttcc ccgagatcga gaaattcgat cacctgccgc ccgcgcgcgt
     1261 cgcgggcgca gccgcgttcg tctcgatcat ggagggatgc agcaagtact gcagcttctg
     1321 cgtcgtgccg tatacccggg gcgaggaagt gtcgcggccc ttcgatgacg tggtggccga
     1381 gattgccggc ctcgcggcgc ggggggtgaa agaagtgacg ctgctcggac agaacgtcaa
     1441 cgcctggcgc ggcgagatcg cccgcgagcc cgccgacttc gccgagttgc tttatttcat
     1501 ttgtgaaata gagggcgtgg agcgcatgcg ctacaccacc tcgcatccgc gcgagttcac
     1561 gcagcgcctg gtcgatgcgc acgcggcaat cgccaagctc gcgccgcacg tgcatctgcc
     1621 ggtgcagtcc ggcgcggacc gcgtgctggc cgcgatgaag cgcggttaca cctcgctcga
     1681 gtaccgctcg attgcgcgcc gcctgaagcg cgcgcgcccc ggcttgagcc tgtcctcgga
     1741 tttcatcgtc ggctttcccg gcgagaccga ggcggatttc gccgccacgc tgaagctcgc
     1801 gtcagagatc ggtttcgacg gctcgttttc gttcctgtac agcccgcggc cgggaacccc
     1861 cgcggccgag ttgcccgatg acacccctca tgaagaaaag ctttcgcggc tgcagcgctt
     1921 gcaggcgcag ctcgattccc aggcgcgtgc catcagcgag agcatggtcg gcaccacgca
     1981 atgtgtattg gttgaaaaac catccaagaa aaacccaaac gagctgtgcg gccggacggg
     2041 caacaatcgc gtcgtgaact tcccgggagc cgagggcctc atcaatacgt tcgccgacgt
     2101 caaaatcacc tgcgcgctgt cgcattcgct gcggggcgag ttgacgtaaa tgatcgcctg
     2161 aatgcgagcc aagcccgtcg aggtcaggct cgatcccgta gacaaccagc gtctcgcgcg
     2221 gctctgcggc gcgctcgacg agaacctgcg ccagatcgag tcggcgctcg acgtcacgat
     2281 tgcgcgccgc ggggcaaaat tcaccgtgcg cggccaccag gcgcagcacg cggcgcaggc
     2341 gctcgagcat ttctacgacc gcgcctccgg cgacctgacg ctcgacgacg tgcaactcgg
     2401 cctcaccgag ctgctgcagg gcaatcaacc ggcgctcgcc gaaggcccgc aattgctgac
     2461 gcgccggcag gacctacacg ggcgcacgcc gcaccagatc gagtatatcg agcacatcct
     2521 ggcccacgac ctcaccttgg gcatcgggcc cgccggcacc ggcaagacct acctggcagt
     2581 cgcctgcggc gtcgacgctc tggagcgtga caaggtgaag cgcatcgtgc tggtgcgccc
     2641 ggcggtcgaa gccggcgagc gtctcggctt tctgccggga gacctcgcgc agaaggtcga
     2701 tccgtatctg cggccgctgt acgacgcgct ctacgacctg atgggcttcg agaagaccgg
     2761 caagctcctc gagcgcggca cgatcgagct cgcgccgctc gcctacatgc gcgggcgcac
     2821 gctcaaccat gccttcgtga tcctcgacga agcgcagaac accacccccg agcagatgaa
     2881 gatgttcctc acgcgcatcg gcttcggcgc caaggcggtg gtcaatggcg acgtcaccca
     2941 gatcgatctg gcgcgcggcc agaagagcgg cctgatcgag gcgcgcagga tcctcgaagg
     3001 cgtgcgcggc atcgccttca cccagttcgg cgcccaggac gtcgtgcgcc acccgctggt
     3061 cgcgcgcatc atcgatgcct acgaagcgaa cccggcacag gagtagcgtc gcggtccaat
     3121 atggcgtgcc acgccgcggt gtgccgagcg cgtccgcg
//
LOCUS       DASPXW010000419         4358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_374511, whole genome shotgun sequence.
ACCESSION   DASPXW010000419 DASPXW010000000
VERSION     DASPXW010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374511"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="VEL04_09555"
     CDS             complement(<1..926)
                     /locus_tag="VEL04_09555"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYT48656.1"
                     /translation="MTARMAARILVVDDQRANVEMMAEVLRARGYEVHTAASGEAALA
                     QVRAVRPDMLVSDILMPGVDGYELCRRLRDDAATTLLPIILVTSLDAQAERVKGLEAG
                     ADDFLSKPLKWEELFARVRSLLRVKALQDELKQLNQQLEERVSDQVAQLERLGRLKRF
                     FSRPVAEAIVTGGEEMLEPHRREITAVFLDLRGFTAFTDRADPDEVLDLLRAYHATLG
                     QRVDEFGGTLEHFAGDGIMIFFNDPLPVENPVERAVRMSLALQQAFTPISTAWKKLGH
                     DVGLGIGIAQGDATLGVIGFENRWEYAAIGNVP"
     gene            complement(923..2035)
                     /locus_tag="VEL04_09560"
     CDS             complement(923..2035)
                     /locus_tag="VEL04_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HYT48657.1"
                     /translation="MSRKARAAICRELHKPVTVEEVSVDPPKRGEVLVKLGACGVCHS
                     DLSAINGTIALPLPLVLGHEGAGVVEEVGAGVTDVEKGDHVVFSFIYMCGKCRFCVAG
                     RPVLCLEQGKALTTPLEGSPRLRDSAGEPLNVFSGCGAMAEYATLSVGNVIRIDPKIP
                     LDCAALVGCGVTTGVGAVFNTAKVVPGSSVAVFGCGGVGLSVIQGARIAGAGRIIAID
                     ALEAKLAMARQFGATEILLSKKDEDLAKALKKMTDGGPDYAFECVGSGELAGTAYRAI
                     RRGGLAVVVGVAKPSDSTSVRTMTLPFEEKTLTGSYFGSCVPRIDFPRMLALYLAGSL
                     KLEELITRRYPIAEAPQAFADLESGRNARGVIVFQG"
     gene            2084..3511
                     /locus_tag="VEL04_09565"
     CDS             2084..3511
                     /locus_tag="VEL04_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HYT48658.1"
                     /translation="MLLKDKLFIGGRWVAPAGRETIEVHNAGNGEVMGRIPAGNEKDA
                     DAAVRAARAALESWKETPVEKRAEFLEKISAGLKARADELARTIAQEVGMPLKLSGRI
                     QAGLPIANFSNYAKLLRDFSFEEKVGNSLVVREPVGVAVAITPWNYPLHQITLKVAPA
                     LAAGCTVVLKPSEVAPFNAFILAEVIEAAGLPAGVFNLVTGYGAGVGEALVRHPGVDL
                     ISFTGSTRAGKRISEIAAQSVKRVALELGGKSASVILDDADLPAAVKGTVNGCYLNSG
                     QTCTALTRMLVPEGKYQEAAKIAAEIAKGFTVGDPLLETTKLGPLSSQAQLERVRGYI
                     KKGLAEGAELVAGGAEPPANAPAGGYYVQPTVFGRVKNSMTIAQEEIFGPVLAIIPYQ
                     DEEDAVRIANDTVYGLAGAVWSKDEDRARRVARRIRAGQVEVNGGAFNMNAPFGGFKQ
                     SGHGREAGRYGLEEFLEYKSLQLKG"
     gene            3512..>4358
                     /locus_tag="VEL04_09570"
     CDS             3512..>4358
                     /locus_tag="VEL04_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYT48659.1"
                     /translation="MRRIEGRLRLITGLAIACFIVPHFLNHSLGVVSIDAMDRLRAVL
                     AAWWRSPPGTVVLYGALVIHFLLALVSLYRRTTLRMPAWEAAQLALGLAVPPLLIAHI
                     VGTRFSWFLLGHTTIGYERIIGLLWSDSWAALRQSLLLLIVWTHFCFGMHYWLRLQRW
                     YPAAQPAAFALALLIPAMALAGFAAAGNELWPAIERVGGMQKYNIEYGSMLPEERARL
                     LAWREGLTWGFWMLLAATLLARVLRTFIGGTYLLRHASGRIIVAPIGQTILEAVRDAG
                     LPHASV"
BASE COUNT          715 a         1470 c         1466 g          707 t
ORIGIN      
        1 ggcacgttgc cgatcgccgc gtactcccag cgattctcga agccgatcac gccgagggtg
       61 gcgtcgccct gcgcgatgcc gattcccagg cccacgtcgt gcccgagttt cttccaggcg
      121 gtcgagatcg gggtgaacgc ctgctgcaac gcgagcgaca tgcgcacggc ccgctccacg
      181 gggttctcga ccggcagcgg gtcgttgaag aagatcatga tgccgtcgcc ggcgaagtgc
      241 tcgagcgtgc cgccgaactc gtcgaccctt tgccccagcg tcgcgtggta ggcgcgcagc
      301 aggtccagca cctcgtcggg gtcggcgcgg tcggtgaagg cggtgaagcc gcgcaggtcg
      361 aggaacaccg cggtgatctc gcgccggtgc ggctcgagca tctcctcgcc gccggtgacg
      421 atcgcctcgg ccaccgggcg cgaaaagaag cgcttgaggc gcccgaggcg ctcgagctgc
      481 gccacctggt cgctgacgcg ctcctcgagc tgctggttca gctgcttcag ctcgtcctgc
      541 agcgccttga cgcgaagcag cgagcgcacg cgcgcgaaca gctcctccca tttgagcggc
      601 ttggagagga agtcgtcggc gcccgcctcg aggcccttga cgcgctcggc ctgcgcgtcg
      661 agcgaggtga cgaggatgat gggcagcagc gtggtcgccg catcgtcgcg cagcctgcgg
      721 cagagctcgt agccgtccac gcccggcatc aggatgtcgg agacaagcat gtcgggtcgc
      781 accgccctca cctgcgcgag cgcggcctcg ccggaggccg cggtgtgcac ctcgtagccg
      841 cgcgcgcgca gcacctcggc catcatctcg acattggccc gctggtcatc gaccaccagg
      901 atgcgagcgg ccattcgtgc agtcatccct ggaatacgat cacgccgcgg gcgttgcggc
      961 ccgattccag gtccgcgaaa gcctgcggcg cttcggcgat cgggtagcgc cgggtaatca
     1021 gctcttcaag cttgaggctg cctgccaggt agagcgcgag catgcgcggg aaatctatgc
     1081 gcggcacgca ggagccgaag tagctgccgg tgagcgtttt ctcctcgaac ggcagggtca
     1141 tggtgcgcac cgaggtcgag tccgagggct tggcgacgcc gaccaccacc gcgaggccgc
     1201 cgcggcggat tgcgcggtag gcggtgcccg cgagctcgcc cgagccgacg cactcgaagg
     1261 catagtccgg tccgccatca gtcatttttt tcaacgcctt cgcgagatct tcatcctttt
     1321 ttgacaataa aatctctgtg gcgccgaact gcctggccat cgcgagcttc gcctccagcg
     1381 cgtcgatggc gatgatgcgc ccggctccgg cgatgcgcgc gccctggatc acgctcaagc
     1441 ccacgccgcc gcagccgaac accgcgaccg aggagcccgg gacgaccttc gcggtattga
     1501 acaccgcgcc gacgccggtg gtgacgccgc agccgacgag cgccgcgcaa tcgagcggga
     1561 ttttggggtc gatcctgatg acgttcccca cggagagcgt ggcgtactcg gccatcgcgc
     1621 cgcagccgga aaagacattg agcggctcgc cggccgagtc gcgcagccgc ggcgagccct
     1681 cgagcggcgt ggtgagcgcc tttccctgct cgaggcacag caccggcctg cccgccacgc
     1741 agaagcggca cttgccgcac atgtagatga acgagaaaac gacgtgatca cccttctcca
     1801 catcggtgac acccgcgccc acctcctcga cgactccggc gccttcgtgg ccgagcacca
     1861 gcggcagcgg cagcgcgatc gtgccgttga tcgccgagag gtccgaatgg cagacgccgc
     1921 aggcgccgag cttcaccagc acctcgccgc gcttcggcgg atcgacgctg acctcctcga
     1981 ccgtgacggg cttgtgcagc tcgcggcaga tcgccgccct ggccttccgg gacatcgcgg
     2041 cagtatagcc ggataaaata tcgggcttcg cgaggagagc ggcatgctat tgaaagacaa
     2101 gctgttcatc ggcggccgct gggtcgcgcc tgccggcagg gaaaccatcg aagtgcacaa
     2161 cgccggcaac ggcgaagtga tgggccgcat cccggccggc aacgagaaag acgccgatgc
     2221 ggcggtcagg gcggcgcgcg ccgctcttga aagctggaaa gagactccgg tggaaaagcg
     2281 cgcggaattt ctcgaaaaaa tctccgcagg cctcaaggcg cgcgcggacg agctcgcgcg
     2341 caccatcgcg caggaagtcg gcatgccgct caagctctcg ggccgcatcc aggccgggct
     2401 gccgatcgcg aatttttcga attacgcgaa gttgctcagg gatttttcgt ttgaggaaaa
     2461 ggtcggtaat tccctcgtgg tacgcgaacc ggtcggcgtc gcggtggcga tcacgccgtg
     2521 gaactatccc ctgcaccaga tcacgctcaa ggtggccccg gcgctcgcag cgggctgcac
     2581 ggtggtgctc aagccctcgg aggtcgcgcc cttcaacgcc ttcatcctcg ccgaggtgat
     2641 cgaggcggcg ggcctcccgg caggcgtctt caacctcgtg acgggctacg gagcgggcgt
     2701 gggcgaggcg ctcgtcaggc atcccggcgt cgatctcatc tcgttcaccg gctcgactcg
     2761 cgccggcaag cggatttcgg agattgccgc gcaaagcgta aaacgcgtcg cgctggagct
     2821 cggcgggaaa agcgcttccg tgatcctcga cgacgccgac ctgcccgcgg cggtgaaggg
     2881 cacggtgaac ggttgctacc tcaactcggg ccagacctgc acggcgttga cccggatgct
     2941 cgtccccgaa ggtaagtatc aagaggctgc gaaaatagcc gccgaaatcg ccaagggctt
     3001 cacggtgggt gatcctcttc tggaaaccac aaaattagga cctctcagct cgcaagcgca
     3061 gctggaacgg gtgcgtggct acatcaaaaa gggcctcgcc gagggcgccg agctggtggc
     3121 cggcggcgcc gagccgcccg cgaacgcgcc cgccggcggc tactacgtcc agccgaccgt
     3181 gttcggaaga gtgaagaaca gcatgaccat cgcgcaggag gagatcttcg gcccggtgct
     3241 cgcgatcatt ccgtaccagg acgaggagga cgcggtgcgc atcgcgaacg ataccgttta
     3301 cgggctcgcc ggcgcggtgt ggtcgaagga cgaagatcga gcgcggcgcg tcgcgcgccg
     3361 catccgcgcc ggccaggtcg aggtcaacgg cggcgcgttc aacatgaacg cgcccttcgg
     3421 cggcttcaag cagtcgggcc acgggcgcga ggccggccgc tacggtctgg aggagtttct
     3481 cgagtacaag tcgcttcagc tcaagggata aatgcgccgc attgagggac ggctgaggct
     3541 catcacgggc ctcgccatcg cgtgcttcat cgtgccgcat ttcctcaacc actcgctcgg
     3601 ggtggtgtcg atcgacgcca tggatcggct gcgcgcggta ctcgcggcct ggtggcgcag
     3661 cccgccgggc accgtggtgc tctacggcgc gctcgtcatc cacttcctgc tcgcgctggt
     3721 gtcgctctac cggcgcacca cgctgcgcat gccagcgtgg gaggcggcgc agctggcgct
     3781 gggcctcgcc gtgccgccgc tgctcatcgc gcatatcgtc ggcacgcgct tctcctggtt
     3841 cctgctcggc cacaccacca tcggctacga gcgcatcatc ggcctgctgt ggtccgattc
     3901 gtgggctgcg ctgcgccagt cgctgctgct gctcatcgtc tggacgcact tctgcttcgg
     3961 catgcactac tggctgcgcc tgcagcgctg gtatcccgcg gcccagccag cggcctttgc
     4021 actggcgctg ctcatcccgg cgatggcgct cgcgggattc gccgccgcgg gcaacgagct
     4081 ctggccggcg atcgagcgcg tcggcggcat gcagaaatac aacatcgagt acggcagcat
     4141 gctgcccgag gagcgcgcgc gcctgctcgc ctggcgcgag ggactgacgt ggggattctg
     4201 gatgctgctc gcggcaaccc tgctggcgag ggtgctgcgc accttcatcg gcggcaccta
     4261 cctgctgcgc catgcctccg gtcgaatcat cgttgcacca atcggtcaaa ccattctcga
     4321 ggcggtgcgc gatgcgggcc tgccgcatgc ctcggtgt
//
LOCUS       DASPXW010000420         6570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_248249, whole genome shotgun sequence.
ACCESSION   DASPXW010000420 DASPXW010000000
VERSION     DASPXW010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6570
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248249"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VEL04_09575"
     CDS             <1..252
                     /locus_tag="VEL04_09575"
                     /inference="COORDINATES: protein motif:HMM:TIGR04255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04255 family protein"
                     /protein_id="HYT48660.1"
                     /translation="EGFLARIVVPHAETGSHIAMSQMLEAANPQTGTVPVIIDIDVFK
                     ETELPVDSGALWEILGKMRHVKNQAFFDSVTPRCLELFK"
     gene            667..1155
                     /locus_tag="VEL04_09580"
     CDS             667..1155
                     /locus_tag="VEL04_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48661.1"
                     /translation="MKFTVKRAWQTVCRKFFALISGNSSPTKQWIPFDVAPGERLSPY
                     LTDRDEYSAHKKIVRFTAFIPPKSLRKSVYWTSGMVEDEIWSLGDEFVAPSRGQIHGR
                     ADFNSYSMSTNIRALAIDLTGKPHPRHADIVGWDDDRKKRRLQAERLADDAVLVMRPP
                     PV"
     gene            complement(1187..1816)
                     /locus_tag="VEL04_09585"
     CDS             complement(1187..1816)
                     /locus_tag="VEL04_09585"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HYT48662.1"
                     /translation="MATSSTPSIDWYFDFVSPYSYLCLHRLADLSSQIAYRPVLFAGL
                     LDHWGQKGPAEIPAKRRWTYRWCTWWAKQLGVPFRFPASHPFNPLPHLRLALAAGCTT
                     QVVTRIFEALWTTGSEATDGFDELCRQLGVDPASLAAPAIKDALKQNTERAAARGVFG
                     VPSFVIEDEVFWGADSIGFVKAFLADPTVLNNTEMHRVDALPIGAARKS"
     gene            1815..2618
                     /locus_tag="VEL04_09590"
     CDS             1815..2618
                     /locus_tag="VEL04_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HYT48663.1"
                     /translation="MAVRIEKKGSVWTVIHARPEARNAMDPASADALVEAFNEFERDP
                     KAAVAVLWGEGGAFCAGWDLKYCATMIEDAKQLHEIDYPLDDRKPIPRGPLGPTRLEL
                     DKPVIAAVAGPAVAGGFELALWCDVRVMEQSAYFGVYCRRWGVPLIDGGTVRLPRIVG
                     MGRAMEIILTGRKVPAEEALRIGACEQVVPDGASRAFAEAMAHEIARFPQACVRADRR
                     SAYLQQGLPLREAMRKEWYNGFPAFQIDGAAGAARFASGKGRHGDFRDI"
     gene            complement(2580..2768)
                     /locus_tag="VEL04_09595"
     CDS             complement(2580..2768)
                     /locus_tag="VEL04_09595"
                     /inference="COORDINATES: protein motif:HMM:NF018630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HYT48664.1"
                     /translation="MTSPCVKVCQMDPRRGLCLGCRRSLDEIARWSSMSDAERDHILS
                     ELSARRLDVSEIPVPPLA"
     gene            complement(2765..3676)
                     /locus_tag="VEL04_09600"
     CDS             complement(2765..3676)
                     /locus_tag="VEL04_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008346504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HYT48665.1"
                     /translation="MGSVPGFVRIVEVGPRDGLQNEPREVPTQVKVELIERLADAGLP
                     AVEATAFVSPKWVPQMADHTEVLERVRIKPGVSYPVLTPNLKGFEAARAAGATEVAIF
                     GAASEAFSRKNINCSIGESLERFRPIALESKKHGIKVRGYVSCVLGCPYEGEVKPERV
                     ADVAAALYEMGCYEVSLGDTIGVGTPGRTQAMLEACAHRVPLERLAGHYHDTYGQALA
                     NVYASLELGVAIFDASVAGLGGCPYAAGASGNVATEDVVYLLHGLGIATGVDLERLAE
                     TGRWICGILGKESSSKVGRALAAKKKK"
     gene            complement(3682..5667)
                     /locus_tag="VEL04_09605"
     CDS             complement(3682..5667)
                     /locus_tag="VEL04_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014618140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HYT48666.1"
                     /translation="MFGKLLVANRGEIACRVIRTARRLGVRTVAVYSAADRDTLHVRL
                     ADEARPIGPAAAKESYLNIDVILAAARASGCEAIHPGYGFLAENAAFAAACARAGLVF
                     IGPAPQAIAAMGDKSAAKRLMEKAGVPLVPGYHGDDQDAALLAKEAARIGYPALIKPC
                     AGGGGKGMRIVTGKKSFGEMLEAARREAKSAFGDERVLIERYLERPRHIEVQVFGDTQ
                     GNVVHLFERDCSVQRRHQKVLEEAPAPGLTPGKRNEMGAAAVAAAKAIGYAGAGTVEF
                     IAEQEGRFYFMEMNTRLQVEHPVTEMITGLDLVEWQLRVACGEPLPLKQDEVRFSGHA
                     IEARLYAEDPAREFLPSTGKLVHLAFPAESAELRVDTGVTQGDEITPWYDPLIAKLIA
                     RGADRAGALARLQAALAGVEIAGVASNVEFLRRVVASHAFATGELDTGLIERNRGELF
                     LENEIKDELLVAAAFAELAEEERAARSRAAGSGDAYSPWHGVDGWRLNQDSHHDFVFM
                     EGAAQHPVRLKFVESGFRISINGKDHALAGERLADGRLLLRLDGRAFKARAVRDAANW
                     HLFCNGEYWRLRLKEELGGFDADTRAGSLAAPMPGKVIKVLTKEGLRVKKDEPLLILE
                     AMKMEHTITAPADGVVKQIHYGAGEQVLEGAELITLE"
     gene            complement(5669..6451)
                     /locus_tag="VEL04_09610"
     CDS             complement(5669..6451)
                     /locus_tag="VEL04_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HYT48667.1"
                     /translation="MLLVDKKGGVARLTLNRPDVRNAFDDALNSLLHKSFVDIENDKA
                     IRVVILAGNGPAFCAGADLNWMKRMAGYGYHENVGDARALAEMLASLDAMSKPTIARV
                     HGPVFAGGTGLVAACDFAVGTAEAKFCFSEAKLGLSPATISPYVIRAIGERAARRYFL
                     TAEVFDAAEAYRIGLLSALVAADQLDSSLDALLKNLLAGGPQALAKIKQLIRAVSGRA
                     LDDALIADTARRIAEIRVSPEGKEGIAAFLEKRKPSWTQGKG"
BASE COUNT         1151 a         2119 c         2115 g         1185 t
ORIGIN      
        1 gagggtttct tggcaagaat agttgtgccg cacgcggaaa ccggctcgca tatcgcgatg
       61 tcacagatgc ttgaggcagc taacccccaa acgggaaccg tgccggtcat cattgacatt
      121 gacgttttca aggagaccga gcttcctgtc gattcgggcg cactatggga aatccttggt
      181 aagatgcgac acgtgaagaa tcaagccttc ttcgatagcg tgactccgag atgcctggaa
      241 ctctttaaat gagtgcagcc gaaagtctgc agccaccttc gtttctgtcg ttcggcactt
      301 cgtcgggggc gagcgacgat gctgctcgca ttgcgtctca gtttcgagag ttctttggtc
      361 gctttattca gtcggccacg ggtagcgagc gcatattgga accccgacag gccatttatg
      421 agctagcagc gcgatgcggg cgtgacaact ggaacggcga gggtgcggag aaaatcactc
      481 aggaagccgc ttcacgtgcg accgatctcc ttgtagcgtt gccctcgtat ctccctgtgc
      541 cagacatatt ccccgatccg tcgggtgcta tctcctttga atggtatcgt cgaccaaaac
      601 accgactcgt gttgagcatc tacggcaacg gtgctgtcga atttgcgggg ctgctcgggg
      661 tggggaatga aatttacggt gaagcgcgca tggcaaacgg tctgccggaa attcttcgct
      721 ttaatctccg gcaactcttc accgactaaa cagtggattc catttgacgt cgcgccgggc
      781 gagcgattgt cgccctatct gacggaccgc gacgagtaca gcgcccacaa gaaaatcgtg
      841 cgctttacag ccttcatacc cccgaagtca ttgcgcaaat ctgtctattg gacatcgggg
      901 atggtggaag atgaaatatg gtctcttggc gatgaattcg tagcgccgtc acgcggacaa
      961 atacacggtc gcgcggattt taattcgtac tcgatgagta caaatattcg agcactggcg
     1021 atcgacctca cgggcaaacc acatccacga catgccgata tcgtcgggtg ggacgatgac
     1081 cgtaagaaac gtcgccttca agcagaaaga cttgctgacg acgccgtgtt ggtgatgcga
     1141 ccgccgccag tctgactagg gctcccgcct aagctaacga gtatggttag ctttttctcg
     1201 ccgcgccgat gggcagggca tcgacgcggt gcatctccgt attgttgagg accgtaggat
     1261 cggcgaggaa ggccttgacg aagccgatcg agtctgcgcc ccagaacact tcgtcttcga
     1321 tcacgaagct gggcacgccg aagacgccgc gcgccgcggc ccgctcggta ttttgcttca
     1381 gggcatcctt gatcgccggc gccgcgaggc tggcggggtc gacgccgagc tgccggcaca
     1441 gttcatcgaa gccatccgtg gcttcgctgc ccgtggtcca gagcgcctcg aagatccgcg
     1501 tcaccacttg ggtcgtgcat ccagcggcga gcgcaaggcg caggtggggt agcgggttga
     1561 acggatgcga ggccgggaag cgaaacggca cgccgagctg cttcgcccac caggtgcacc
     1621 agcggtaggt ccagcgacgc ttggcaggaa tctcggccgg gcctttctgg ccccagtgat
     1681 cgagcagccc ggcaaacagc accgggcggt acgcaatctg ggacgacagg tctgcgagac
     1741 ggtgcaggca gaggtacgag tagggcgata cgaaatcgaa ataccaatct atgctaggcg
     1801 tcgaggaggt ggccatggcg gtaaggatcg aaaagaaggg gagcgtctgg acggtgattc
     1861 acgcgcgccc cgaggcgcgc aatgcaatgg atccggcaag cgccgatgcg ctggtggaag
     1921 cattcaacga attcgagcgt gatccgaagg cggccgtggc ggtgctctgg ggcgagggcg
     1981 gtgcattctg cgccggctgg gacctcaagt actgcgccac catgatcgag gacgcgaagc
     2041 agctgcatga gatcgactat ccactcgacg atcgtaagcc aattccgcgc ggaccgctcg
     2101 ggccaacgcg gctcgagctc gacaagcccg tcatcgccgc cgtcgccgga ccggcggtgg
     2161 cgggcggctt cgagcttgcc ctttggtgcg atgtgcgcgt gatggagcag tcggcctact
     2221 tcggcgtgta ctgccgccgc tggggcgttc cgctcatcga cggcggcacg gtgcggctgc
     2281 cgcgcatcgt cggcatgggt cgcgccatgg aaatcatcct taccggccgc aaagtaccgg
     2341 cggaggaggc gctgcgcatc ggcgcctgcg agcaggtggt acccgacgga gcctcgcgcg
     2401 ccttcgcaga ggccatggcg cacgagatcg cgcgctttcc gcaggcctgc gtgcgcgccg
     2461 accggcgctc ggcttacctg cagcaaggcc tgccgctccg cgaggcgatg cgcaaggaat
     2521 ggtacaacgg ctttcccgcc ttccagatcg acggcgccgc aggcgcggca aggtttgcct
     2581 caggcaaggg gcggcacggg gatttcagag acatctaatc tccgtgctga cagttctgag
     2641 aggatatggt cgcgctcggc atcgctcatc gagctccagc gcgcgatctc gtcgagcgag
     2701 cgccggcagc cgaggcacaa gccgcgccgc ggatccatct ggcagacctt gacgcaggga
     2761 ctggtcattt cttttttttc gcagccagcg cccgcccgac cttcgaggag gattctttgc
     2821 cgaggatgcc gcagatccac cggcccgttt cggcgaggcg ctcgagatcg acgccggtcg
     2881 cgattcccag gccatgcagc aggtagacca catcctcggt agcgacattg ccggatgccc
     2941 cggcggcgta ggggcagccg ccgagcccgg cgaccgacgc gtcgaaaatc gccacgccaa
     3001 gctcgagcga tgcgtacacg ttggcgagcg cctggccgta ggtgtcgtgg taatggccgg
     3061 cgagccgctc gagcggcacg cggtgcgcgc aggcctcgag catcgcctgc gtcctgccgg
     3121 gcgtgccgac accgatggtg tcgcccagag acacctcgta gcagcccatc tcgtatagcg
     3181 ccgcagcgac gtcggcaacg cgctcgggct tcacctcgcc ctcgtagggg cagccgagca
     3241 cgcacgacac atagccgcgg actttgatgc catgtttttt tgattccagg gcaatgggac
     3301 gaaagcgctc tagcgactcg ccgatcgagc aattgatgtt cttgcgcgag aacgcttccg
     3361 acgcggcccc gaaaatcgcc acctcagtcg cgccagcagc gcgcgcggct tcgaacccct
     3421 tgaggttcgg cgtcagcacc gggtaggaca cgccgggctt tatgcggacg cgctcgagca
     3481 cctcggtgtg gtcggccatc tgcggcaccc atttcggcga cacgaaggcg gtcgcctcga
     3541 ccgccggcag gccggcgtcg gcgagccgct cgatcagctc gaccttgact tgggtcggca
     3601 cctcgcgagg ctcgttctgc agcccgtcgc gcggccccac ttccacgatc ctcacgaaac
     3661 cgggcacgga ccccatttct ctcattccag ggtgatgagt tccgcgcctt cgagcacctg
     3721 ctcgccggcg ccgtagtgga tctgcttcac cacgccgtcg gccggcgcag tgatggtgtg
     3781 ctccatcttc atcgcttcga ggatgagaag gggctcatct ttcttcaccc tcagtccctc
     3841 ctttgtaagc actttgatga cctttcccgg catcggcgca gcgagcgagc cggcacgggt
     3901 gtcggcgtcg aagccgccaa gctcttcctt tagccggagc cgccagtatt cgccgttgca
     3961 gaacaggtgc cagttggctg cgtcgcgcac cgcgcgcgct ttgaatgcgc ggccgtccag
     4021 ccgcagcagc agccggccgt ccgccagccg ctcgccggcg agcgcgtggt ctttcccgtt
     4081 gatcgagatt ctgaagccgc tttcaacgaa tttcagccgc accgggtgct gtgccgcgcc
     4141 ttccatgaaa acgaaatcat ggtgcgagtc ctggtttagc cgccaaccgt cgacgccgtg
     4201 ccagggagaa tacgcatcgc ccgagccggc tgcgcggctg cgcgcggcgc gctcttcctc
     4261 ggcgagctcg gcgaaggccg ccgcaacgag cagttcatct ttaatttcat tttctaaaaa
     4321 caattcgcca cgattgcgct cgatcaggcc ggtatcgagc tcgcccgtgg cgaaagcgtg
     4381 gctcgcaacg acgcggcgca ggaactcgac attgctcgcc acgcccgcga tctcgactcc
     4441 ggcgagcgcg gcttgcaggc gggcgagcgc gccggcgcgg tcggcaccgc gtgcgatcag
     4501 cttggcgatc agcgggtcgt accacggcgt gatctcgtcc ccctgcgtga cgcccgtgtc
     4561 gacccgcaac tcggcgcttt cggcgggaaa ggcgagatgc acgagtttcc cggtcgaggg
     4621 caggaactcg cgcgccgggt cttcggcgta gagacgcgct tcgatcgcat ggcccgaaaa
     4681 cctgacttcg tcttgtttca ggggaagcgg ttcaccgcac gccacccgca gctgccattc
     4741 gacgagatcg aggccggtga tcatctcggt cacggggtgc tcgacctgca gccgcgtgtt
     4801 catctccatg aagtagaagc gcccctcctg ctcggcgatg aactccaccg tgccggcccc
     4861 ggcatagccg atcgccttcg ccgcggcgac cgctgccgct cccatttcat ttctcttgcc
     4921 gggcgtgagg ccgggcgcag gcgcttcctc gagcactttc tggtggcgac gctgcaccga
     4981 gcagtcgcgc tcgaagaggt ggacgacatt tccctgcgta tcgccgaaca cctgcacttc
     5041 gatgtggcgc ggccgctcga ggtagcgctc gatcagcacg cgctcatcgc cgaaggccga
     5101 tttcgcctcg cgccgcgcgg cctcgagcat ttcaccgaaa gacttcttcc cggtgacgat
     5161 gcgcatgccc ttgccgccgc cgcccgcgca gggcttgatg agcgcggggt agccgatgcg
     5221 cgccgcttct ttcgcgagca gcgccgcgtc ctggtcgtcg ccgtgatacc ccggcacgag
     5281 cggcacgccg gccttttcca tcagccgctt ggcggccgac ttgtcgccca tcgccgcgat
     5341 cgcctgcggc gccgggccga tgaaaacgag gccggcgcgc gcgcaggccg cggcgaaggc
     5401 tgcgttctcg gcgagaaagc cgtagccggg atggatcgcc tcgcacccgg aggcgcgcgc
     5461 cgcagcaaga attacatcga tattcaaata actttctttt gctgcagctg gccctatggg
     5521 ccgcgcctcg tcggcgagcc tgacgtgcag cgtatcgcgg tccgcagcgg aatacaccgc
     5581 gaccgtacgc acgccgagcc ggcgcgcggt gcggatcacg cggcaggcga tctcgccgcg
     5641 gttggcgacc aggagcttgc cgaacacgct agcctttgcc ctgggtccag gaaggcttgc
     5701 gcttctcgag gaacgccgcg atgccctcct tgccctccgg cgagacgcgg atctcggcga
     5761 tgcgccgggc ggtatccgcg atcagcgcgt cgtccagcgc ccgcccggag acggcccgga
     5821 tgagctgctt gattttcgcc aacgcctgcg gcccgcccgc gaggaggttc ttcagcaacg
     5881 catcgaggct cgaatcgagc tggtcggcgg cgaccagggc ggacagcagg ccgatgcggt
     5941 acgcctctgc cgcgtcgaac acttccgcgg tcaggaagta gcgccgcgcc gcccgctcgc
     6001 cgatcgcccg gatcacatag gggctgatgg tcgccggcga aagcccgagc ttcgcctcgg
     6061 aaaagcagaa cttcgcctcg gccgtgccga ccgcgaagtc gcaggccgcg accagcccgg
     6121 tgccgcccgc aaacaccggg ccgtgcacgc gcgcgatggt cggcttcgac atggcgtcga
     6181 gcgacgccag catctcggcg agcgcgcggg cgtcgcccac gttctcgtgg tagccgtagc
     6241 ccgccatgcg cttcatccag ttgaggtccg ccccggcgca gaacgccggg ccgttgccgg
     6301 caaggattac gacccggatg gccttgtcgt tttcgatatc gacaaaactc ttgtgtaaaa
     6361 gcgagttaag agcgtcgtcg aacgcattcc ggacgtcagg ccggttgagc gtgaggcgcg
     6421 cgactccgcc ctttttatcg acgagcagca tcacattctg aaaatgccga acttggtttc
     6481 ttcgatcggg gcgttgagcg aggccgagat cgcgaggccg agcaggcggc gcgtgtcggc
     6541 cggatcgacg atgccgtcgt cccacagccg
//
LOCUS       DASPXW010000421         5535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1635719, whole genome shotgun sequence.
ACCESSION   DASPXW010000421 DASPXW010000000
VERSION     DASPXW010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5535
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1635719"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VEL04_09615"
     CDS             complement(<1..244)
                     /locus_tag="VEL04_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HYT48668.1"
                     /translation="MAGAAAPCRQEVSRLPRLNLLEGKSVVVTGAGGGIGRDFALAMA
                     AAGARVVVNDLGTSVRGEGKDASPAQKVVDEIKAQGG"
     gene            complement(190..969)
                     /locus_tag="VEL04_09620"
     CDS             complement(190..969)
                     /locus_tag="VEL04_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HYT48669.1"
                     /translation="MSDLSQVQIPPMRGRVSPEEWQTRIDLAACYRLVHHYGMDDLVY
                     NHISARVPGEEGHFLINAYGMTYDEITASSLVKIDFDGRIVQDSGTGYGINQAGFVIH
                     SAVHKGRPDVGCVIHTHSIAGMAVSALDCGLLPITQTAMYFDGVGMHEYESVAIDLDE
                     QQRLVRDLGSFKAMILRNHGLLAVGATVSEAFTNLFWLERACKAQVAAMACNTKLHLP
                     AQDVIKKTNHLYRPETRRRWGPLEWPALLRLADRKYPGFRD"
     gene            1044..2576
                     /locus_tag="VEL04_09625"
     CDS             1044..2576
                     /locus_tag="VEL04_09625"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HYT48670.1"
                     /translation="MAKPETSLAWLSVAAVLLSFFLALGRAPLFDVDEGAFSQATLEM
                     FQRGDYLSTYLNGEPRYDKPILVYWLQAASVLAFGPTGFAFRLPSAVCATLWCALVFL
                     FTRAACGATAAWLAAACTATSVGVFIIGRAATADALLNLLIAGSLLAAWLHLKSGERK
                     WLYAAFAAAALGVMAKGPVAVLIPFCVTLAFCLVRRDFRTWLRAAFDPRALLVFALIA
                     LPWYLIIFYKEGWAFVEGFFLKHNVGRFGSPLQGHGGSLVYYIPVALIASMPHTGLLL
                     KALMKTRELWRDDLQCYLLLWFGFVFAFFSLSGTKLPHYMLYGMTGMFILMAVQAASL
                     QSRLLLIAPTVLFFGFLLAFPALTEMLAARVGDAYYREALGDAGSHFTAAYYIFAAAS
                     IAFCVWLMAERRIAVPLKIVGCGVLSVAALAAFVVPALGTMLQEPVREAALLARSSGY
                     PVIMWGINAPSFSVYYGRPTPSRTPRPGDIVITKAKRLAELPGFDTLYARKGVVLVRI
                     KE"
     gene            2573..4345
                     /locus_tag="VEL04_09630"
     CDS             2573..4345
                     /locus_tag="VEL04_09630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYT48671.1"
                     /translation="MNFSPRHPLVWGVPAAALLGMALIAGTGSNTAGFRALNALGSGG
                     QAFWSAVTILGDTAVALSLALLAARRRPQLLWALLPAALIATAWVHGWKKTYDLNRPL
                     ATLERQEVQVIGPELRYSSFPSGHATTAFTAAGLCVLGLRLGGWSAALLVIAALVALS
                     RVVVGAHWPLDVLAGAFGGWIAAALGLICAERWRFGLRPGIQALAALVCAACAVALVA
                     GYDGRYPDAGWLARAIGVVALAGFALTFVLRRGVPMETADLPTLRAAALSGESVSIVI
                     PVYNEAANLRDLVDRVGAALAPSGASFELILVDDGSNDSSPALLEEMAAARQWLKPLY
                     LIRNYGQSVALQAGFDHARGDFIVTLDGDLQNEPDDIPRLLVLMREKPQIDVISGWRK
                     DRKDAVLSRKIPSLLANSLISGVTGVRLHDYGCALKVYRAKVIHDIKLYGEMHRFIPA
                     LAAEVGARILEVPVSHHARTRGTSKYGIGRTLRVVLDLLWVKFLLRFLHRPMHAFGGI
                     GILLLALGFATLAYLAYEKLALGQDIGGRPLLLLGVLLALMGGQIMATGLIGELLIRI
                     YHEPQGRRLYVLRAAPDKPPRPMP"
     gene            4342..5208
                     /locus_tag="VEL04_09635"
     CDS             4342..5208
                     /locus_tag="VEL04_09635"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HYT48672.1"
                     /translation="MKRALRVIAGAALLGAAIWYADPRALWGALAGVNPWLFGAAVAV
                     AILSNLVSALRWSLIARGLGLVSPLPSALLMYARGITANMMLPGSVVSGDVLRSVQLS
                     RLGNPLVASAWSVFLDRFSGLWMLCAMSTLAALGVALSRPEIPMLLGYAAILAAAVVA
                     PLLPLPLPKTGRWGELAKGLQRSRPVLLASAGHSCAVQCLAAGTLWICGLSVGLALSY
                     PVMLAAAAPIFIMASLPIGIAGFGAREAAAVAVLGMVGVPGEQAFTTGLLFGLAAVVQ
                     GVAAAPWFFAKA"
     gene            5229..>5535
                     /locus_tag="VEL04_09640"
     CDS             5229..>5535
                     /locus_tag="VEL04_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HYT48673.1"
                     /translation="MKVLVSVKRVVDFNVKVRVKADGSGVETANVKMSMNPFDEIAVE
                     EAVRLKEKGAASEIIVVSCGVQACQETLRTALAIGADRAILVETGVELQPLAVAKILK
                     "
BASE COUNT          745 a         1828 c         1886 g         1076 t
ORIGIN      
        1 tgccgccctg cgccttgatt tcgtcgacga ctttctgcgc ggggctggcg tctttgcctt
       61 cgccgcgcac cgaggtgccc agatcgttaa ccacgactcg ggcgccggct gcggccatcg
      121 ccagggcgaa atcgcgtccg atgccgccac ctgcgccggt gacgacgacg ctcttgcctt
      181 cgaggaggtt caatcgcgga agccgggata cttcctgtcg gcaaggcgca gcagcgccgg
      241 ccattccagc gggccccagc gcctgcgggt ctcgggccgg tagaggtggt tggtcttctt
      301 tatgacgtcc tgcgcaggaa ggtggagctt cgtgttgcac gccatggccg ccacctgggc
      361 cttgcacgcg cgctcgagcc agaagagatt ggtgaaagcc tcggagacgg tagcgcccac
      421 cgcgagcagg ccatggttcc gcaggatcat cgccttgaag ctgccgaggt cgcgcaccag
      481 gcgctgctgc tcgtcgaggt cgatcgccac gctctcgtat tcgtgcatgc cgacgccgtc
      541 gaagtacatc gcggtctgcg tgatcggcag caggccgcag tcgagcgccg aaacggccat
      601 gccggcgatg gagtgcgtat ggatcacgca gcccacgtcc ggccgcccct tgtgcacggc
      661 gctgtggatg acgaagccgg cctggttgat gccatagccg gtgccggagt cctgcacgat
      721 gcggccgtcg aagtcgatct tcaccaggct ggaagccgta atttcatcgt acgtcatacc
      781 gtaggcattg atcaggaaat ggccctcctc gcccggcacg cgcgccgaaa tgtggttgta
      841 cacgaggtcg tccatgccgt agtggtgcac caggcggtag cacgccgcga gatcgatgcg
      901 cgtctgccat tcctcgggcg aaacgcggcc gcgcatcggc ggaatttgca cttgcgacag
      961 atcgctcatc gaagggcctc ctgagcggat gatacgataa gcgcccgcgc ctgatgcaga
     1021 ctcgcccatt ctcgtcgctc cggatggcga agccggagac ctcgctcgcg tggctcagcg
     1081 tcgccgcggt gcttctcagc ttcttcctcg ctctcggccg cgcgccgctg ttcgacgtcg
     1141 acgaaggcgc cttcagccag gcgacgctcg agatgttcca gcgcggcgat tacctctcga
     1201 cctatctcaa cggcgagccg cgctacgaca agccgattct ggtgtactgg ctgcaggcgg
     1261 cgagcgtgct cgccttcggg ccgaccgggt tcgcgttccg gctgccgtcc gcggtgtgcg
     1321 cgacgctgtg gtgcgcgctg gtgttcctgt tcacgcgcgc cgcctgcggc gctacggcag
     1381 cgtggctggc cgcggcctgc accgcgactt cggtgggtgt tttcatcatc gggcgcgctg
     1441 cgaccgcgga cgcgctcctc aacctgctca ttgccgggtc gctgctcgca gcatggctgc
     1501 acctcaagtc aggggagcgc aaatggctgt acgcggcgtt cgccgccgcg gctctcgggg
     1561 tgatggcaaa gggacccgtt gcggtactga tacctttctg cgtgacgctc gctttctgcc
     1621 tggtgcggcg cgacttcagg acctggctgc gcgccgcatt cgacccgcgg gcgctgctgg
     1681 ttttcgcgct gatcgcgctg ccctggtacc tgatcatctt ctacaaggaa ggctgggctt
     1741 tcgtcgaagg cttcttcctc aagcacaacg tcgggcgctt cggcagtccg ctgcaggggc
     1801 atggcggcag cctggtctat tacatcccgg tggcgttgat cgcttcgatg ccgcacacgg
     1861 gtctcttgct aaaagcactc atgaaaactc gggagctctg gcgcgacgat ctgcagtgct
     1921 atctgctgct gtggttcggg ttcgtgttcg cttttttttc gctctccggg acgaaactgc
     1981 cgcattacat gctctacggc atgaccggaa tgttcatcct gatggcggtg caggcggcga
     2041 gcctgcagtc gcgcctgctg ctgattgcgc cgacggtgct gtttttcggg tttctcctgg
     2101 cgtttcccgc gctgacggaa atgctcgctg caagggtcgg cgatgcctat taccgtgaag
     2161 cgctgggcga tgccggcagc catttcactg cggcctatta catcttcgct gcggcgagca
     2221 tcgcattttg cgtctggctg atggccgaac gccgcattgc cgtgccgctc aagatcgtcg
     2281 gctgcggcgt gctttcggtc gcagcgctgg ccgcgttcgt cgtaccggcg ctgggcacga
     2341 tgctgcagga gcctgtcagg gaggcggcgc tgcttgcccg cagcagcggc tatccggtca
     2401 tcatgtgggg gatcaatgcg ccgagcttca gcgtgtatta cggccggccg acgccgagcc
     2461 gtacgccgcg tccgggcgac atcgtcatca ccaaggcgaa acggctggcc gagctgccgg
     2521 gtttcgacac tctctacgcg aggaagggcg tggtcctggt tcgcatcaag gaatgaactt
     2581 ctccccgcgc catccgctcg tctggggcgt gccggccgcc gctctgctcg gcatggcgct
     2641 catcgccggg accggcagca ataccgctgg cttccgggcg ctgaatgcgc tcggctcagg
     2701 cggccaggcg ttctggtcgg cggttaccat tctcggagac accgctgtcg cgctgtcgct
     2761 cgcattgctc gcggcgcgcc ggcgcccgca gctgctgtgg gcgctgcttc ccgcggcgct
     2821 gatcgccacg gcatgggtgc acggatggaa aaaaacctat gacctgaacc ggccgctcgc
     2881 cacgctcgag cggcaggaag tccaggtgat cggcccggag ctgcgctaca gttcgtttcc
     2941 ctcggggcac gccacgacgg cctttaccgc cgccgggctt tgcgtcctgg ggctgcgcct
     3001 gggcggctgg agcgctgcgc tgctcgtcat cgccgcgctg gtagcgcttt cccgtgtggt
     3061 ggtgggcgcg cactggcccc tcgatgtgct ggccggagct ttcggcggct ggatcgcggc
     3121 agcgctcggc ctgatttgcg ccgagcgatg gcgcttcggc ctgcggccgg gcatccaggc
     3181 gctggccgcg ctggtatgcg ccgcgtgcgc cgtggcgctg gttgcaggct atgacggtcg
     3241 ctatccggac gcgggctggc tcgcccgcgc gatcggcgtg gtggcgctcg ccggctttgc
     3301 tcttaccttc gtgcttcgcc gtggagtccc tatggaaact gctgacctcc ccaccctgcg
     3361 cgccgcggcc ctcagcggag aaagcgtttc catagtcata cccgtctaca acgaggcagc
     3421 gaatctgcgc gatctggtcg atcgcgtcgg cgcggcgctc gccccgagcg gcgcgtcttt
     3481 tgagctgatc ctggtcgacg acggctccaa cgacagctcg ccggcgctgc tcgaggagat
     3541 ggcggcggcg cgccagtggc tgaagcccct gtacctgatc cgtaactacg gacagtcggt
     3601 ggcgctgcag gcgggcttcg atcatgcgcg cggcgatttc atcgtcaccc tggacggtga
     3661 cctgcagaac gagccggacg acatcccgcg gctgcttgtg ctgatgcgcg agaagccgca
     3721 gatcgacgtc atctcgggct ggcgcaagga ccgcaaggac gccgtgctgt cgaggaaaat
     3781 cccgtcgctg ctcgccaact cgctcatctc cggggtcacc ggcgtgcggc tgcacgacta
     3841 tggctgcgcc ctcaaggtgt atcgcgccaa ggtgatccac gacatcaagc tctacggcga
     3901 gatgcaccgt ttcatcccgg cgctcgccgc ggaggtcggg gcgcggatcc tggaagtgcc
     3961 ggtcagccac catgcgcgca ctcgcggcac ctcgaagtac ggcatcggcc gcaccctgcg
     4021 cgtggtgctc gacctgttgt gggtcaagtt cctgctgcgc ttcctgcacc gcccgatgca
     4081 cgccttcggc ggcatcggca tcctgctgct cgcgctcggc ttcgccacgc tggcttatct
     4141 cgcctacgaa aagctggcgc tcggccagga catcggcggc cggccgctgc tgctgctcgg
     4201 cgtgctgctc gcgctgatgg gtggccagat catggcgacc gggctcatcg gcgagctgct
     4261 gatccgtatc taccacgagc cgcagggacg ccgcctctac gtgctgcgcg ccgcgccgga
     4321 caagccgccg agaccgatgc cgtgaagcgc gccctgcgcg tgatcgccgg cgcggcgctc
     4381 ctcggcgcgg cgatctggta cgccgatccc cgggcgctgt ggggcgcgct ggcgggcgtc
     4441 aacccctggc tgttcggcgc tgcggtggcg gtcgcgatcc tcagcaacct ggtgtccgcg
     4501 ctgcgctggt cgttgatcgc ccgcggcctg ggcctcgtct ctccccttcc cagcgcactc
     4561 ttgatgtacg cgcgcggtat cacggcgaac atgatgctgc cgggctcggt cgtctcgggc
     4621 gacgtgctgc gcagcgtgca gctctcgcgc ctcggcaatc cgctggtcgc ctcggcgtgg
     4681 tcggtgttcc tcgatcgctt cagcgggctg tggatgctgt gcgcgatgtc gaccctggcg
     4741 gcgctcggcg tggcgctatc gcggcccgaa attcccatgc tgttgggcta tgcggcgatt
     4801 ctcgcggcgg ctgtggtggc gcccctgctt cccttgccgc tgccgaagac cgggcgttgg
     4861 ggcgagctcg cgaaaggcct gcagcgttcg cggccggtgc tgcttgcctc ggccgggcac
     4921 tcgtgcgcgg tgcaatgcct tgccgccggc acgttgtgga tttgcggcct gtcggtggga
     4981 ctcgcgctat cctacccggt gatgctcgcg gcagcggcac cgatcttcat catggcgtcg
     5041 ttgccgatcg gcatcgccgg cttcggggcg cgcgaagccg cggcggtcgc ggtgctcggt
     5101 atggtcggcg ttcccggcga gcaggctttc accaccggcc tgctgttcgg gcttgccgca
     5161 gtggtccagg gagtcgcggc ggcgccgtgg ttcttcgcca aggcatagct gcggctataa
     5221 tttttccggt gaaagtcctg gtgagtgtca agcgggtcgt cgatttcaac gtcaaggtgc
     5281 gcgtcaaggc cgacggcagc ggcgtcgaga ccgccaacgt caagatgtcg atgaatccgt
     5341 tcgacgagat cgcggtcgag gaagcggtgc ggctgaagga aaaaggcgcc gcgagcgaga
     5401 tcatcgtcgt ctcgtgcggc gtgcaggcct gccaggagac gctgcgcacc gcgctcgcca
     5461 tcggcgccga tcgcgccatc ctggtcgaaa ccggcgtcga actgcagccg ctggcggtgg
     5521 cgaagatcct gaagt
//
LOCUS       DASPXW010000422         5239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_877459, whole genome shotgun sequence.
ACCESSION   DASPXW010000422 DASPXW010000000
VERSION     DASPXW010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5239
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877459"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="VEL04_09645"
     CDS             complement(<1..892)
                     /locus_tag="VEL04_09645"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HYT48674.1"
                     /translation="MGSNPIGHPNLAPPRKWRRPALAKLATPSKAFCAIFTAALAQVG
                     ANTRGVVQSTHPEYLHQMRVGLRRSRSALRAFRELVPKNAAKPIAARLRGLMSALGAA
                     RDWDVFCESLLRIGMHEPHRAPAMAQLFARARGKRAAARRRARETAASPKLQAFLLRA
                     LRCVNARPWKRRPEKTERSLGAFAASSLERLHRKTLRQAQDIDWSDPERRHRLRIWMK
                     RLRYACDFFATSFAGVAARPYIKRLEALQDILGDLNDVAVARRLLAEIAPPGSAPEHA
                     AAYTRLTLATRERMLVISLRA"
     gene            complement(865..939)
                     /locus_tag="VEL04_09650"
     tRNA            complement(865..939)
                     /locus_tag="VEL04_09650"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(905..907),aa:His,seq:gtg)
     gene            complement(957..1033)
                     /locus_tag="VEL04_09655"
     tRNA            complement(957..1033)
                     /locus_tag="VEL04_09655"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(997..999),aa:Arg,seq:tct)
     gene            complement(1051..1127)
                     /locus_tag="VEL04_09660"
     tRNA            complement(1051..1127)
                     /locus_tag="VEL04_09660"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1091..1093),aa:Pro,seq:tgg)
     gene            1246..1671
                     /gene="dksA"
                     /locus_tag="VEL04_09665"
     CDS             1246..1671
                     /gene="dksA"
                     /locus_tag="VEL04_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HYT48675.1"
                     /translation="MAVELTRKSFTPYAAKKGEEYMNAHQRRHFRKILEALKSELSQE
                     IDRTVHTMQDEATVFADPNDRASQESDMALELRNRDRERKLIKKIDETIARIDANEYG
                     YCESCGVEIGLKRLEARPTATLCIDCKTLDELRERQVAK"
     gene            complement(1678..3786)
                     /gene="pnp"
                     /locus_tag="VEL04_09670"
     CDS             complement(1678..3786)
                     /gene="pnp"
                     /locus_tag="VEL04_09670"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HYT48676.1"
                     /translation="MNPTKKTFPYGNHQVTLETGEIARQAHGAVVCTMDDTVVLATVV
                     ASKQPKIGQDWFPLTVDYVEKTYAAGKIPGGFFKREGRPSEKETLTSRLIDRPIRPLF
                     PDSFYNEVQVVATVMSLNPEVDSDIAAMLAVSAALTLSGIPFNGPIGACRVGYVDGSY
                     VLNPTLTQLKESKLNLVVAGTEQGVLMVESEALELDEEKMLGAVMFGHEQMQTAIQAI
                     HELAETAAKPAWDWQAPPKNDELIARIKELAETELREAFRIKQKKARAEAIDAIWKRV
                     FEAIGVGREGGPNANVAKEICFALESSIVRGQILDGEPRIDGRDTRTVRPVSVRVGVL
                     PRTHGSALFTRGETQALVTVTLGTGRDEQIIDALAGEYRERFMMHYNMPPYATGETGR
                     MGPSKRREIGHGRLAKRALVGLLPTAEEFGYSIRVVSEITESNGSSSMASVCGGSLAL
                     MDAGVPVKAHVAGIAMGLIKEGKRFAVLSDILGDEDHLGDMDFKVAGTEKGVTALQMD
                     LKIESINQGIMKVALDQAREARLMILQIMKQAIEGSRAELSSFAPRIIKMKINPDKIR
                     DVIGKGGVVIRGIQEETGTTIEIEDDGTIAIACVSAEGGEAAKKRIQELTADVEVGKI
                     YEGTVLRLLDFGAIVQLLPGRDGLLHISQISHERVNTVSDHLKEGQTVKVKVLEADDK
                     GRVRLSMKAISAQQTAKTNG"
     gene            complement(3864..4133)
                     /gene="rpsO"
                     /locus_tag="VEL04_09675"
     CDS             complement(3864..4133)
                     /gene="rpsO"
                     /locus_tag="VEL04_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HYT48677.1"
                     /translation="MALQVVQKSQVVKQYQRSGKDTGSPEVQIALLTERINGLTEHFK
                     THVKDFHSRRGLLVMVSQRRKLLDYLKRKDADKYRALIERLSLRK"
     gene            complement(4196..5080)
                     /gene="truB"
                     /locus_tag="VEL04_09680"
     CDS             complement(4196..5080)
                     /gene="truB"
                     /locus_tag="VEL04_09680"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008349234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HYT48678.1"
                     /translation="MIDGALLVDKPVGLSSNAVLQQVKRLVGARKAGHAGTLDPLASG
                     LLVILFGEATKFAGLLLESDKEYRATLKLGETTSTADAEGAVLEAKNFHVSNEQMIAV
                     LERFRGEIEQLPPMHSALKRDGVPLYKLARRGQTVERSRRRVEIFELESTGFHPPLLE
                     LRVRCSKGTYIRTLAEDIGAALGTGAHLAALRRTAAGRFLIEDAATPDEIARLNETQR
                     QGRLLPMERLLADLPRVELDAALELRLRNGQALKISGLQEGVSALYTADGALIGLGRA
                     EEGVLRPLRLTQAAEKHR"
BASE COUNT          882 a         1720 c         1673 g          964 t
ORIGIN      
        1 cggccctgag ggaaatgacg agcatgcgct cgcgcgtggc gagcgtcagg cgggtatacg
       61 cagcagcatg ctcgggtgcg ctgccgggcg gagcaatctc ggcaagcagg cgtcgcgcca
      121 cggcgacgtc gttgaggtct ccaagaatgt cttgtagcgc ctccaagcgc ttgatgtaag
      181 gccgcgccgc gacaccggca aaggatgtag cgaagaaatc gcaggcgtag cgcaaccgct
      241 tcatccagat gcgcagccgg tgacggcgtt ctgggtcgct ccagtcgatg tcctgcgcct
      301 gcctcagcgt tttgcgatgc agccgctcga gcgacgacgc cgcgaaggcg cccagcgagc
      361 gctcggtttt ttctggccgt cgtttccatg gtcttgcgtt cacgcaacgc agggcgcgca
      421 agaggaaggc ctgcagcttg ggcgacgccg cggtctcgcg cgcgcgccgt cgcgctgccg
      481 cgcgcttgcc ccgggcgcgc gcgaaaagct gggccatcgc cggcgctcga tgcggctcgt
      541 gcattccgat gcgcagcaga ctctcgcaaa aaacgtccca gtctcgcgcc gctccgagag
      601 cagacatcag accgcgcagg cgcgctgcga tcggcttcgc cgcgttcttc ggcaccagct
      661 cgcggaacgc gcgcagcgct gagcgcgagc gcctcaagcc gacgcgcatc tggtgcagat
      721 actcggggtg cgtgctttgg acaacgccgc gcgtgtttgc tccgacctgc gcgagcgccg
      781 cagtaaagat ggcgcaaaac gccttgctcg gcgtagcgag cttcgcgagt gccggccggc
      841 gccacttgcg cggcggcgcg aggttggggt ggccgatggg attcgaaccc acaacaaccg
      901 gaatcacaat ccgggactct acctttgagc tacggccacc atcgcatccg ccgctttggc
      961 ctgcccggcg ggactcgaac ccacaacccc cagcttagaa ggctggtgct ctatccggtt
     1021 gagctacggg cagtcccggt gaggcagacc tggtcggggt gagaggattt gaacctctga
     1081 catcttgctc ccaaagcaag cgcgctacca ggctgcgcca caccccgaaa aaggcggcaa
     1141 atgatactct tagcgcatgt tttgggcaac gcgattggct tgcgacaggg ggtcttttct
     1201 gctacaaaga gcgcctttct tttttatcag gccgcgcaaa aaataatggc ggtggagctc
     1261 acccggaaaa gtttcacgcc ctacgccgcg aaaaagggcg aggagtacat gaacgcccat
     1321 cagcgcaggc atttccgcaa gatcctggaa gcgctcaagt cggagctgtc gcaggagatc
     1381 gaccgcacgg tccacaccat gcaggacgag gcgacggtgt tcgccgatcc gaacgaccgc
     1441 gccagccagg agtccgacat ggcgctcgag ctgcgcaacc gcgaccgcga aagaaagctc
     1501 atcaagaaga tcgacgaaac catcgccagg atcgacgcca acgaatacgg ctactgcgag
     1561 agctgcggcg tcgagatcgg gctgaagcgg ctggaagccc ggcccacggc gacgctgtgc
     1621 atcgactgca agacgctcga cgagctgcgc gagcgccagg tcgccaagta aggaaagcta
     1681 tccgttcgtc tttgcggttt gctgcgcgct gatcgccttc atggaaaggc gcacgcggcc
     1741 cttgtcgtcg gcttccagca ccttgacctt caccgtctgg ccttccttca ggtgatctga
     1801 cacggtgttg acgcgctcgt ggctgatctg cgagatatgc agcaggccgt cgcgccccgg
     1861 cagcagctgc acgatggcgc cgaagtcgag cagcctgagc accgtgccct cgtagatctt
     1921 gccgacctcg acgtcggcgg tgagctcctg gatgcgcttc ttcgccgcct cgccaccctc
     1981 ggccgagacg caggcgatcg cgatggtgcc gtcgtcctcg atttcgatgg tggtaccggt
     2041 ctcttcctgg atgccgcgga tcaccacgcc gcccttgccg atgacatcgc ggatcttgtc
     2101 cgggttgatc ttcatcttga tgatgcgcgg cgcgaagctc gagagctcgg cgcgcgagcc
     2161 ttcgatcgcc tgcttcatga tctgcaggat catcaggcgg gcctcgcgcg cctggtcgag
     2221 cgccactttc atgatgccct ggttgatcga ctcgatcttc aggtccatct gcagcgccgt
     2281 cacgcccttc tcggtgcccg cgaccttgaa gtccatgtcg ccgaggtggt cctcgtcgcc
     2341 gaggatgtcc gagagcacgg cgaagcgctt gccctccttg atcaggccca tcgcgatgcc
     2401 ggcgacgtgc gccttgaccg gcacgccggc gtccatcagc gcgaggctgc cgccgcacac
     2461 cgaggccatc gacgaggagc cgttggattc cgtgatctcg gaaactacgc ggatcgagta
     2521 gccgaattcc tcggccgtcg gcagcaggcc gacgagcgcg cgcttggcaa gccggccgtg
     2581 gccgatctcg cggcgcttcg acggtcccat gcggccggtc tcgccggtgg cgtagggcgg
     2641 catgttgtag tgcatcatga aacgctcgcg gtactcgccc gccagcgcgt cgatgatctg
     2701 ctcgtcgcgc ccggtgccga gggtcacggt gacgagcgcc tgcgtctccc cccgcgtgaa
     2761 caaagcggag ccgtgagtcc gaggcagcac gccgacgcgc acgctcaccg gccgcacggt
     2821 gcgcgtgtcg cggccgtcga tgcgcggctc gccatcaagt atctggccgc gcacgatgga
     2881 tgactcgagc gcgaagcaga tctccttggc gacattggcg ttgggcccgc cttcccggcc
     2941 gactccaatg gcctcgaaca cgcgcttcca gatcgcatcg atcgcctccg cgcgtgcctt
     3001 cttctgcttg atgcgaaacg cctcgcgcag ctcggtctcg gcaagctcct tgatgcgcgc
     3061 gatcagctcg tcgttcttcg gcggcgcctg ccagtcccag gccggcttgg cggccgtctc
     3121 agcgagctcg tggattgcct ggatcgcggt ctgcatctgc tcgtggccga acatcaccgc
     3181 gccgagcatc ttctcctcgt ccagctcgag cgcctcggat tccaccatca gcaccccctg
     3241 ctccgtgccg gcgacgacca gattcagctt cgactccttc agctgggtga gggtcgggtt
     3301 gaggacgtag ctgccgtcga cgtagccgac gcggcaggcg ccgatcgggc cgttgaaggg
     3361 gatgcccgag agcgtcagcg ccgccgatac cgccagcatc gccgcaatgt cggaatcgac
     3421 ctcggggttc agcgacatca ccgtggcgac gacctggacc tcgttgtaga agctatcggg
     3481 gaacagcggc cggatcggcc ggtcgatgag ccgcgaggta agggtctcct tttccgatgg
     3541 gcgcccttcg cgcttgaaga agccccctgg gatcttgccc gcggcgtaag ttttctcgac
     3601 gtagtcgacg gtcaacggga accagtcctg cccgatcttc ggttgcttcg atgcgaccac
     3661 ggtcgcgagc accacggtgt cgtccatggt gcagaccacg gcgccatggg cctgccgtgc
     3721 gatctcgccg gtttcgaggg tgacctggtg gttgccgtaa gggaaagtct tcttggtcgg
     3781 gttcaaggga tctcctcagt caggaatgca ccagcaaaga cgctcaccgg gaacgcgagc
     3841 gctcagtgcc accgcatcgg gagttatttg cgcaggctga gccgctcgat cagggcgcgg
     3901 tacttgtccg cgtccttgcg cttcaggtaa tcgagcagct tgcggcgctg gctcaccatg
     3961 acgagcagtc cgcgccggga gtgaaaatcc ttgacgtgcg tcttgaagtg ctcggtgagg
     4021 ccgttgatgc gctcggtgag gagcgcgatc tgcacttcag gcgagccggt gtctttgccg
     4081 gaacgctggt actgcttgac gacttgcgac ttctgtacca cctgcaatgc cattcggctc
     4141 tctctctcga tgtaagcgcg cgattctaac cgaagcgctg ttccggtcac aaggtttatc
     4201 gatgtttttc agccgcttgt gtaagcctga ggggccgcag cacgccctcc tcggcccggc
     4261 cgaggccgat cagcgcgccg tccgccgtgt acagcgccga tacgccttcc tgcaggccgc
     4321 tgattttcaa tgcctgccca ttgcgcaagc gcagctccag cgcggcgtcg agctcgactc
     4381 gcggcagatc ggcaagcagc ctctccatcg gcagcaaccg cccctgacgc tgcgtttcat
     4441 tcaacctggc aatctcgtcc ggggtcgctg cgtcctcgat cagaaagcgg cccgcggcag
     4501 tacggcgcaa ggccgcgagg tgcgccccgg tgccaagcgc tgcgccgata tcctcggcga
     4561 gagtgcggat gtaggtgcct ttgctgcagc ggacccgcag ctcgagaagc ggggggtgaa
     4621 agcccgtgct ctcgagctcg aaaatctcga cccgccggcg gctgcgctcc accgtctggc
     4681 cgcgccgcgc cagcttgtag agcggcaccc cgtcgcgctt cagcgccgag tgcatcggcg
     4741 gaagctgctc gatctctcct cggaatcttt ccaggaccgc gatcatctgt tcattcgata
     4801 cgtgaaaatt ttttgcttcc aggaccgcgc cttcggcatc ggcggtcgat gtggtctcgc
     4861 cgagcttgag cgtggcgcga tattccttgt cggactcgag cagcaggccg gcgaatttgg
     4921 tcgcttcgcc gaacagaatc accaacagcc ccgaggcgag cggatccagc gtgccggcgt
     4981 gacccgcttt gcgcgcgcca acaagcctct ttacctgctg caaaacagcg ttcgagctca
     5041 gcccgaccgg cttgtcgacg agcagcgcgc catcgatcac ttcttcagga cgctgtcgat
     5101 caggcgcgag aggcggtcgc cgcgctcaac cgactcgtcg tattcgaagc gcagctcggg
     5161 cgtggtgtag agcttgatgc gcttcgccag ctgcgagcgc agaaatcccg cggcgcgacg
     5221 caggccatgc agcgtgtct
//
LOCUS       DASPXW010000423         2323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_628941, whole genome shotgun sequence.
ACCESSION   DASPXW010000423 DASPXW010000000
VERSION     DASPXW010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628941"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="VEL04_09685"
     CDS             <1..644
                     /locus_tag="VEL04_09685"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYT48679.1"
                     /translation="AGALTALQQAQTRAPARADLHKLQGDIALKVGDRQGARAAYRAA
                     LDLDAGFVQVWLDLGRLHEHDEEWVEAQRAYEAALEALPTFHEAALALADLLRRSGSV
                     RAAVVRLAEMLEQDPYDMDALLLLGRALLDDKRNAEALEAFRRALKFDPQHTGALFYL
                     GATLARLQRYGEAVDAWERVTRIDPGGPFAQRARMHARTAVDLQHIFSTSDAA"
     gene            648..1784
                     /locus_tag="VEL04_09690"
     CDS             648..1784
                     /locus_tag="VEL04_09690"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HYT48680.1"
                     /translation="MPIEGPLRELGIHDVFQLLDLSRKTGALRVTSELRHNEGKIYFD
                     NGVVVSAEIRSNPHPLGALLLRTGKITEADLERARDMQTRQGDRRRLGEILVSLGVIT
                     QREVERQIRFQIEEVVFEVMSWNEGYFSFAEEAESKVPTEVTVRIPVEALLMEGARRI
                     DEWSRIENRIPHVGVVPSLAPTPEGGGGELDLLPPEWEVLALIDGERSIRALATELGR
                     SDFEVAKTLFGLESAGVIVLMDPGTAKRGRPGGAADLPELVAKAERALQAKDLDAARA
                     AAEQAGSMQPHDATIHLLLGRIHLAQGHGSAAAEELRRALRLDPLLAAAHRQLGFALV
                     SMGRFGEAVASWDEWERLARSPDEAAHRADVQRAREAARVFSHG"
     gene            1777..>2323
                     /locus_tag="VEL04_09695"
     CDS             1777..>2323
                     /locus_tag="VEL04_09695"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYT48681.1"
                     /translation="MADDIRALSAQLAQDPQSLVFLRLGEALRRKGQLDAALRVAMNG
                     LDRHPHLADAHDLYARILTDQRDYERAFDEWDMAVRIAPHHTGALKGLAFLYFKVGDL
                     AQAEAHLEAARKVEPDDASIDQAIAMMRKGAAPPAPEPSAAPSPPAAPALDEARVFAG
                     LEGANEGLLLIDGAGRVLGGAL"
BASE COUNT          368 a          778 c          838 g          339 t
ORIGIN      
        1 cggccggggc attgacggcg ctgcagcagg cgcagacccg cgcgccggca cgcgccgatt
       61 tgcacaagct gcagggcgac atcgccctca aagtcgggga tcggcagggc gcgcgcgcgg
      121 catatcgggc ggcactcgat ctcgacgcgg gctttgtgca ggtgtggctc gacctcgggc
      181 gcctgcacga gcatgacgag gaatgggtgg aagcgcagcg cgcgtatgaa gccgcactcg
      241 aggcgctgcc cacattccac gaagccgcgc tagcgctcgc cgacctgttg cggcgctccg
      301 gcagcgtgcg cgcggccgtg gtgcggctgg ccgagatgct ggagcaggat ccctacgaca
      361 tggacgccct cctgctgctc ggccgcgcac tgctcgacga caagcgcaac gccgaagccc
      421 tggaggcgtt ccggcgcgcg ctcaaattcg atccccagca caccggcgcc ttgttctacc
      481 tcggcgcgac gctcgcgcgg ctgcagcggt acggcgaagc ggtggatgcg tgggagcgcg
      541 tgacgcgtat cgatccgggc gggccgttcg cgcaacgggc gcgcatgcac gcgcgcaccg
      601 cggtcgacct gcagcacatc ttctccacct cggacgcggc gtagcgcatg ccgatcgagg
      661 gaccgctacg cgagctcggg atccacgacg tcttccagct gctcgacctg agccggaaga
      721 cgggggcgct gcgggtcacc tcggagctgc gccacaacga gggcaagatc tacttcgaca
      781 acggcgtcgt cgtgtcggcg gagatccgca gcaacccgca cccgctcggc gccttgttgc
      841 tgcgcaccgg caaaatcacg gaagccgatc tcgagcgcgc gcgcgacatg cagacgcggc
      901 agggggacag gcgccggctc ggcgagatcc tcgtgtcgct cggcgtgatc acgcagcgcg
      961 aggtcgagcg ccagatccgt ttccagatcg aggaagtcgt cttcgaggtg atgagttgga
     1021 acgagggcta cttctcgttc gccgaagagg ccgagagcaa ggtccccacc gaagtgacgg
     1081 tccgcattcc cgtggaggcg ctgttgatgg agggggcgcg ccgcatcgac gagtggtcgc
     1141 gcatcgagaa ccggattccg cacgtgggcg tcgtgccgtc gctggcgcca acacccgaag
     1201 gagggggcgg ggaactcgac ctcctacccc cggaatggga ggtgctcgcg ctgatcgacg
     1261 gcgaacgatc catccgggcg ctggcgacgg agctcggacg atcggatttc gaggtggcca
     1321 agacgttgtt cggtctcgag agtgccggcg tgatcgtgct catggacccc gggacggcta
     1381 agcgcggccg tccgggcggc gcggccgacc tgcccgagct ggtcgccaaa gccgagcggg
     1441 ccctgcaggc caaggacctc gacgccgcgc gcgccgccgc cgagcaggcg gggagcatgc
     1501 agccgcacga cgcgacgatc catctgctgc tggggcggat tcatctcgcg cagggacacg
     1561 ggtccgcggc ggccgaggag ctgcggcgcg cgctgcggct cgatccgctg ctcgccgcgg
     1621 cgcatcgcca gcttggattt gcgctcgttt cgatgggtcg cttcggtgaa gccgtcgcca
     1681 gttgggacga gtgggagcgg ctcgctcgca gccccgacga agcggcgcac cgggcggacg
     1741 tgcaacgcgc gcgcgaggcg gcccgagtgt tcagccatgg ctgacgatat ccgcgcactc
     1801 tcggcccagc tcgcccagga tccgcagagt ctcgtgttcc tgcggctggg cgaagcgttg
     1861 cgccgcaagg gccagctcga tgcggcgctg cgggtggcga tgaatgggct cgaccggcat
     1921 ccgcatctcg ctgatgcgca cgacctgtac gcgcgtatcc tgacggacca gcgcgactat
     1981 gagcgcgcat tcgatgagtg ggatatggcc gtccgcattg ccccccacca tacgggcgcg
     2041 ctgaaaggac tggcgttcct gtatttcaag gtgggcgacc tggctcaagc ggaggcacat
     2101 ctcgaggcgg cgcgcaaagt cgagcccgac gacgcgagca tcgatcaagc gatcgccatg
     2161 atgcggaaag gcgcggctcc gcctgcacca gagccaagcg ctgccccctc gccgcccgcg
     2221 gctccggcgc tcgacgaggc ccgcgttttc gccggtctcg agggtgccaa cgaaggcctc
     2281 ctgctcatcg acggcgccgg tcgggtcctc ggcggtgcat tgc
//
LOCUS       DASPXW010000424         3243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_878326, whole genome shotgun sequence.
ACCESSION   DASPXW010000424 DASPXW010000000
VERSION     DASPXW010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878326"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            277..1642
                     /gene="rimO"
                     /locus_tag="VEL04_09700"
                     /pseudo
     CDS             277..1642
                     /gene="rimO"
                     /locus_tag="VEL04_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224690.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
     gene            complement(1679..2971)
                     /locus_tag="VEL04_09705"
     CDS             complement(1679..2971)
                     /locus_tag="VEL04_09705"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HYT48682.1"
                     /translation="MSRKKGDQGTSRPAWYEAGLDHVWLPYAQMKTVRAPLPIARTHG
                     CRIVLCDGRELIDGIASWWTACHGYNHPHIRKAVERQLALMPHIMFGGLAHEQALTLA
                     RRLAARIPGDLTRVFFSESGSVAVEIAMKMAVQFWLNQGVRKRKKFLAFKGGYHGDTT
                     GAMAVCDSEDGMHARFHGLLPEHHIVELPQSEESAAIFARSLERHAEELAGIIVEPLV
                     QGAGGMRFHWPSVLQRLRTAADRYQLLLIFDEIFTGFGRTGSLFACEAAGVVPDIMTL
                     SKALTGGTLPLAVTVARDKVFDAFWSDDPTRALLHGPTFMGNALSCAAANASLDLFER
                     EPRLRQVADIASALERGLAPCRGLPGVKDVRVKGAIGAVELDRIDDLEALRARFVEEG
                     VFVRPFGSIVYLTPAFTIGEDELAKLTGVVTKVVGKRG"
     gene            3026..>3243
                     /locus_tag="VEL04_09710"
     CDS             3026..>3243
                     /locus_tag="VEL04_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012732740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HYT48683.1"
                     /translation="MHSLDEFATAKLGELERSSLRRALVDTTRVTGIWLLRNGRRLLS
                     FCCNDYLNLTHHPAVKEAAIEALLVYGVG"
BASE COUNT          576 a         1081 c         1047 g          539 t
ORIGIN      
        1 cgtcaaggag gaggtgcggt gcggtcttta cccacacgag cgcggcggcc cgactggggg
       61 gcccaacctc caaaaagaaa acccgccagg gatttctccc ggcgggccta catcgatcgc
      121 gccctcgctc ccgccggtac atccgcggtc ggaagctcgg tcgcgaacaa cctctatata
      181 ggccgcaatg atgaatattc aatgacggcg gtccggaacc ggcgcgcgga gaccaggccg
      241 cttgctcctg acggcgcgct cgggtagccc ctcgccatgg aaaacagaca tcccgcgccg
      301 cgcgcgccgc gagtgagttt cgtctcgctc ggctgcccga aggcgctggt cgacagcgag
      361 cgcatcatga cccgcctgcg cgcggagggc tatgaactcg ctcgttccca tgccggcgcc
      421 gacctcgcgg tggtcaacac ctgcggcttt ctcgacagcg cccaggcgga atcgctcgcc
      481 gccatcggcc agtgagaacg gcaaggtgat cgtgaccggc tgcatgggcg ccgagccaga
      541 aaagatcacc gcgcgatatc ccggagtgct cgcgatcact ggtccgcagc agtacgagag
      601 cgtggtcgcc gcggtgcatc gcgcgctgcc gccgcgccac gacccgtttg tcgatctcct
      661 gccccccgag ggcatcaagc ttacgccccg gcattatgcc tatctgaaga tttcggaggg
      721 ctgcaacaat cgctgcagct tctgcatcat cccgaaactg cggggcgatc tggtctcgcg
      781 gcccgccgcc gacgtgctgc gcgaggccga gcgtctcatc gccgcgggcg tgaaggagct
      841 tctggttatt tcgcaagaca cttcggccta cgggacggac ctcaaatatg cggcgagcgc
      901 gtggaagggg cacgaggtgc aggcgaaatt tctcgatctc gcccgcgcac ttggcgagct
      961 cggcgcctgg gtgcggctgc attacgtcta cccctacccg catgtggacg aggtcatccc
     1021 gttgatggca gagggcaagg tgctgcccta tctcgatgtc ccgtttcagc atgcctcacc
     1081 tgccgtgttg cgccggatga aacggccggc cgcgcaggag aaaaccctgc ggcgcatcgc
     1141 ccgctggcgc gacatctgcc ccgatctcac catccgctcg accttcatcg tcggctttcc
     1201 cggcgagagc gaagaggatt tccgacgctt gctcgattgg ctcgatgagg cagagctcga
     1261 ccgcgtcggc tgcttccgtt acgagccggt tgcgggcgcg cccgccaacg agcttgcgcc
     1321 cgcagtcccc gacgcggtga aggaggagcg ctggcatcgc ttgatggggc atcagcaggc
     1381 gatctccgcg cgacgcctca ggcgcagagt cggcacgcgc caacaggtga tcatcgacca
     1441 agttcacggc tcctgcgaca agtcggaccc ggtgggcgcc gccgcgaccg tcgccaaggg
     1501 gcgcacgcgc ggcgacgcgc cgcagatcga cggcgttgtc ctcgtcgcaa gccgccgccc
     1561 gctgcgccgg ggcgagatcg cgacggtgcg catcgaacgc gccgacgaat acgacctgca
     1621 tggcacggtg gtggggtttt gagcccctgc tggcgcatca gcaatgggat tacggatcct
     1681 agccgcgttt gcccaccacc ttcgtcacca ctcccgtcaa tttcgcgagc tcgtcctcgc
     1741 cgatcgtgaa cgccggggtg agatagacga tggacccgaa cggccggacg aataccccct
     1801 cctccacgaa ccgcgcgcgc agggcctcga gatcgtcgat gcggtcaagc tccacggcgc
     1861 cgatcgcccc cttcacccgc acgtccttga cgcccggaag gccgcggcaa ggcgcaaggc
     1921 cgcgctcgag ggcggacgcg atatcggcaa cttgccgcag gcgcggttcg cgctcgaaca
     1981 ggtcgagcga ggcgttggcg gcggcgcagg agagcgcgtt tcccatgaag gtcggcccgt
     2041 gcaggagcgc gcgggtggga tcgtcggacc agaaagcgtc gaaaactttg tcgcgcgcga
     2101 cggtgaccgc gagcggcagc gtcccgccgg tcaatgcttt cgacagggtc atgatgtcgg
     2161 gcacgacgcc ggctgcttcg caggcgaaga ggcttccggt tcgtccgaag ccggtgaaaa
     2221 tttcatcgaa gatcaacagc agctggtagc ggtccgccgc ggtgcgaagg cgctgcagca
     2281 ccgacggcca atgaaagcgc atgccgccgg cgccttgcac caacggctcc acgatgatgc
     2341 ccgcgagttc ttcggcgtga cgctcgagag agcgcgcaaa gatcgcagcg ctctcctcac
     2401 tctgcggcag ctcgacgatg tgatgctcgg gcaacaggcc atggaagcgc gcatgcatgc
     2461 cgtcttcgga atcgcacacc gccatcgccc cggtcgtatc gccatggtag ccgcccttga
     2521 aggcgaggaa tttcttgcgc ttgcgcacgc cctggttcag ccagaactgc accgccatct
     2581 tcatggcgat ctcgacggcg actgagccgg actccgagaa gaagacgcga gtcagatcgc
     2641 ccgggatgcg tgccgcaagt cgacgggcaa gcgtgagcgc ctgctcgtgg gcgagcccgc
     2701 cgaacatgat atgcggcatc agcgccagct ggcgctcgac cgccttgcgg atatgggggt
     2761 ggttatagcc gtgacaggcg gtccaccacg atgcgatccc gtcgatcagc tcgcgtccat
     2821 cgcagagcac gatgcgacaa ccatgggtgc gagcgatagg caacggcgct cgcaccgtct
     2881 tcatctgcgc atacggcagc cagacgtggt cgagcccggc ctcataccac gccggccgcg
     2941 aagttccttg atctcccttt tttcgagaca ttcggcgctt tcaatgcaaa gcttaccgac
     3001 atgacgggtt gaggtcgatg cgaggatgca ttcactcgat gaatttgcga ccgccaagct
     3061 cggcgaactc gaacgcagca gtctacgccg tgcgctcgtc gacaccaccc gggtgacggg
     3121 aatttggctg ctgcgcaacg gccgccggct gttgtcgttt tgctgcaacg attacctcaa
     3181 cctgacccat cacccggccg tcaaggaggc ggccatcgag gcgttgctcg tctatggtgt
     3241 agg
//
LOCUS       DASPXW010000425         8295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1636611, whole genome shotgun sequence.
ACCESSION   DASPXW010000425 DASPXW010000000
VERSION     DASPXW010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8295
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1636611"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VEL04_09715"
     CDS             <1..270
                     /locus_tag="VEL04_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48684.1"
                     /translation="PGPGGAPTTPVGDCGGANPPCLTINNSRYPTTTKRAALILAGRA
                     LAGQVRPSAALADYLEGENLSPLDEVLRRGASSSAINDRVVAVDP"
     gene            270..1106
                     /locus_tag="VEL04_09720"
     CDS             270..1106
                     /locus_tag="VEL04_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HYT48685.1"
                     /translation="MQGARAQRQSLAIGFTLVELAVVLAIVGLLLGSLMYTLSAQTEQ
                     RNFEETRRRLEQARELVLAYAIVNGQLPCPARSNSAGAEVRDAASRRCWDGAVEDYYG
                     GALAGGVTGGFLPAATIGYTQIDSGGFAVDAWQNRIRYAVAKSIAAGTCAGAFTNPHW
                     VNAMNLRNNGMACQPGDLLICKSATGITPSACGGTANQIMTQGLIVAIVFSTGKNGAI
                     SGGSGADEAANLDGAGNANPVFVFHTPTPADFANGEFDDQFTWITVGELYGKMIAAGI
                     VP"
     gene            complement(1114..2841)
                     /locus_tag="VEL04_09725"
     CDS             complement(1114..2841)
                     /locus_tag="VEL04_09725"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYT48686.1"
                     /translation="MQPATLTEPFFFRIANVVWGFSQRYWRLVVVAMLALLHVAVFRG
                     VADPWARALLLAHLGLLLLWQPFMRAEQRISPMQGLILSLLAFAVMMYLDWWLLAFWV
                     VVIAGLVGGKVYQHHARWQRRCYLVVLAYLLALLAVAILPEIAPRREIAPEIRRAAEY
                     LLPLAFLVIALFPAEAEPAEAAQVIDFFYSVFLMLLLVVVILGSFTFMTLTRSSYLAA
                     LTSTVFLTAGTMLLVGLAWNPRSGGGLGVFFARYLFSIGLPVEKWLQELAELLQVEAR
                     PEQFLAEAVTALQRMPAVAGVSWRAGDIQGERGERTPHAVEFASSELALTIYSRFRLG
                     PALQWHLHLLAQVLAEFYVAKLREAQLREASYLQAVHETGARTTHDIKNLLQSLNVLC
                     SVAGREEYRDSAQLQGLVRRQLPMVAQRLSETLDRLQHPEPARESYVPARSWWEALAR
                     QYRGEGVEFQAVEPPAAARLPRSLFDSVADNLIGNALAKRAAEPETRVRVALECGGRI
                     ELRVCDSGSAVPPEIEASLLRSPVSSRGGLGIGLYQAARQAESGGYHLALETNRDGEV
                     CFALSGPAA"
     gene            2877..3638
                     /locus_tag="VEL04_09730"
     CDS             2877..3638
                     /locus_tag="VEL04_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYT48687.1"
                     /translation="MSAAILARLVLLEARRGGLPWLAAGSIALGLALAAFLSQVALTE
                     SRALQVAVVAALLRACAVFLIAAQVASSVLREINDKGLELMLSLPLPRSTHYLGRLAG
                     FAALGVLLAVVFAAPLLAWAPAGAVALWGLSLACETALIAAAALFFAMTLAQLVPAIA
                     ATAGLYLLARSIAAIQAIASGPLAEPSLPSELARMAVGGVALLLPPLDAVTRTDWLLY
                     GAPGPGAYGAALAGLTIYAAVLIAAGLFDFHRRSA"
     gene            3665..4351
                     /locus_tag="VEL04_09735"
     CDS             3665..4351
                     /locus_tag="VEL04_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48688.1"
                     /translation="MPAWLWAALAATLALQIAWRSASPAPSLGESDLPPPPGPQALRL
                     ASFGEPEAAARIAMLYLQAFDLGGANAAPYQKLDYRRLAGWLRSVLALDPRSAYPLFS
                     AARVYAEVPDAERCRVMLEFIYEEFLLDPNRRWPWLAHAALLAKHRLKDLPLARRYAA
                     AIDRRTTAQNVPLWARQMEIFILEDMNELEAARIMLGGLLASGAVRDPDEARFLRHRL
                     EELEARQPVK"
     gene            4494..5168
                     /locus_tag="VEL04_09740"
     CDS             4494..5168
                     /locus_tag="VEL04_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HYT48689.1"
                     /translation="MKKTQGFTLVEIAIVLVIIGLLLGGILKGQEMITQAKIKNVIAD
                     FSGISAAYHGYVDRYKKIPGDDPCAGGAVTLGNCGTTTGRWTGATAGDGNGQVAGAYN
                     STTATDESRLWWDHLRRAGFVSGVGDQQPFNALSGQIGVQTGNAASPPVAALGGFAGI
                     IICSANLPDKVAIAADTQMDDGVIDKGTVRGQLQTTPNPAINAAADTVAYAENGTNVY
                     VICRSL"
     gene            complement(5217..7190)
                     /locus_tag="VEL04_09745"
     CDS             complement(5217..7190)
                     /locus_tag="VEL04_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HYT48690.1"
                     /translation="MDHAVSLQSVLVLLASSVAAVALCRSLRLPPIVGYLATGLALGP
                     HAAGLVSDREETRHLAEFGVVFLMFSIGLEFSLPKLIAMRRVVFGLGMLQVAATIAFA
                     VGACMALGGAWQTGIALGGIIAMSSTAIVSKLLAERGELDSAHGREVIGVLLFQDLAV
                     VPLLVLIPALGQPVDAIGVAVAVALAKAALALAIVIVGGPKLMRAWLGAVARRRSNEL
                     FVLNVLLVTLLLAFLTGLAGLSLVLGAFLAGMLISETEYRFQVEEDIKPFRDVLLGLF
                     FITVGMMLDLRLIFGEFLLVLMFFVLLVGLKLVLIAALARAFRSPPGTALRVALALAQ
                     GGEFGFVLLPLAGAAGVVSDEILQALLAAMILSMLATPFVIGASDRIVMRLSRSEWMQ
                     RSLELHRVASQSMEAERHVIILGYGRNGQRLARLLDAEGVRYVALDLDPERVREAAAA
                     GDAVVFADSSRREALMAAGIARAAAVVVTFADTAAAVRVLAHIHGLNSSVPVIVRARD
                     ESDIERLTAAGATEVVPEAFESGLMLASHTLVMVGVPLSRVMRRVSQVRDQQYRLLRG
                     LFHGPSEWRGDGEAAARLHAVTLDANAHATGRPLAELGLEALGVEVRAVRRRGAKTKL
                     SAAEAGALQAGDVVVLLGTPEVLDAAEQRLLQG"
     gene            7208..8233
                     /locus_tag="VEL04_09750"
     CDS             7208..8233
                     /locus_tag="VEL04_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HYT48691.1"
                     /translation="MIKAAPKRQPSGEKSGEPGIKVNPLDLARRVLSIEADAVRALAE
                     RIDERFLAAVALIRDRSGRVVVSGIGKSGHIARKIASTLSSTGTPAYFVHPAEASHGD
                     LGMIESGDVFLAISYSGASAELLEIVPLVKRRGAKLIVMTGRADSALAREADVLLDAR
                     VEQEACPHNLAPTASTTAALALGDALAVALLDARGFSADDFARSHPRGQLPRQALVHI
                     ADVMRSGNDLPTVQCGVLAMQAVVEMSRCRMGMTAVIDERRQVRGIFTDGDLRRSLEK
                     RVDFGATRVDQLMTAAPKTIRPEALAIEAVQLMESHKVNQLLVVNERGELVGAFNMHD
                     LFRAKVI"
BASE COUNT         1382 a         2898 c         2812 g         1203 t
ORIGIN      
        1 cctggcccgg gcggcgcgcc gaccacaccg gtcggtgatt gcggcggagc caatcctccc
       61 tgcctgacca tcaacaattc gcgctatccg acgacgacca agcgcgctgc gctgatcctc
      121 gccgggcgcg cgcttgccgg acaagtgcgc cccagcgcag cgctggcaga ctacctcgaa
      181 ggcgagaacc tgtcgccact cgatgaggtc ctgaggcgcg gcgcgtcgag ttccgcgatc
      241 aatgaccgcg tcgtggcagt cgatccttga tgcaaggcgc gagagcacaa cgacagtcgc
      301 ttgcgatcgg attcacgctg gtcgagctcg ccgtggttct tgcgattgtc ggtcttctgc
      361 ttggcagcct tatgtacacg ctctcggcgc agaccgagca gcgcaacttc gaggagacgc
      421 gacggcgcct cgagcaggcg cgcgagctcg tgctcgctta tgccatcgtc aatggacaac
      481 tgccctgccc ggcgcgaagc aatagcgccg gggccgaagt gcgcgacgca gccagccgcc
      541 gctgctggga cggagccgtc gaagactatt acggaggagc gctcgctggc ggggtcacgg
      601 gaggtttcct gccggctgcg actatcggct acacgcagat cgacagcggg ggcttcgcgg
      661 tcgatgcctg gcaaaaccgg atccgctatg cagtggcgaa gtccatcgcc gccggcacct
      721 gcgccggcgc ctttaccaat cctcattggg tcaacgcgat gaacctcagg aacaacggca
      781 tggcgtgcca gcccggcgac cttctgatct gcaaatcggc aaccggcatc acgccgagcg
      841 cgtgtggagg tacagccaac cagataatga cccagggcct cattgtggcc atcgttttct
      901 cgaccggaaa gaacggcgcg atcagcggcg gcagcggcgc cgacgaggcg gcgaacctgg
      961 atggcgccgg aaacgccaac ccggtgttcg ttttccacac tccgaccccc gccgacttcg
     1021 cgaacggcga gtttgacgat cagttcacct ggattactgt aggcgagctg tacggaaaaa
     1081 tgatcgccgc cggcatcgtg ccctgagcga gcctcaggcg gccgggccgc tcagcgcgaa
     1141 gcacacctcg ccgtcccggt tcgtctcgag cgcgaggtgg taaccgcccg actcggcctg
     1201 gcgcgcggcc tggtagagac cgatgccgag cccgccgcgc gagctcaccg gcgagcgcag
     1261 cagactcgcc tcgatttccg gcggcacggc gctgccgctg tcgcagacgc gtagctcgat
     1321 gcgcccaccg cactcgagcg cgacccgcac gcgggtttcg ggttcggcgg cccgcttggc
     1381 aagcgcgttg ccgatcaggt tgtcggcgac gctgtcgaac agcgagcgcg gcaggcgcgc
     1441 cgcggcaggc ggctcgacgg cctggaattc gaccccttcg ccgcggtact ggcgcgcgag
     1501 cgcttcccac cagctgcgcg ccggcacgta gctctcgcgg gcgggctcgg gatgctgcag
     1561 ccgatcgagc gtctccgaaa ggcgctgcgc caccatcggc agctggcggc gcaccagccc
     1621 ctgaagctgg gccgagtcgc ggtactcctc gcgcccggcc accgagcaca gcacgttcag
     1681 cgattgcagc aggttcttga tgtcgtgcgt ggtgcgcgcc ccggtctcgt gcaccgcctg
     1741 caggtagctc gcctcgcgca gctgcgcctc gcgcagcttg gcgacgtaga actcggcgag
     1801 cacctgtgcg agcaggtgca ggtgccactg cagcgccggg ccgagcctga agcgcgaata
     1861 gatggtgagg gcgagctcgc tgctcgcgaa ctctacggcg tgcggcgtgc gctcgccgcg
     1921 ctcgccctga atatcgcccg cgcgccagct gacccccgcc accgccggca tgcgctgcag
     1981 cgccgtcacc gcctcggcga gaaattgctc tggacgcgcc tcgacctgga gcagctcggc
     2041 gagctcctgc agccatttct cgaccggcag gccgatcgag aagagatacc tcgcgaagaa
     2101 cacgcccagc ccgccgccgc tgcgcgggtt ccaggcgagc ccgacaagca gcatcgtgcc
     2161 cgcggtgagg aacacggtcg aggtgagcgc cgcgaggtag ctcgagcggg tgagggtcat
     2221 gaaggtgaag ctgccgagga tcacgaccac cagcagcagc atcaggaaca cgctgtagaa
     2281 gaaatcgatc acctgcgccg cctcggcggg ctcggcctcg gccggaaaga gggcgatcac
     2341 caagaaggcg agcggcagca ggtattcagc cgccctgcgt atttcggggg cgatctcgcg
     2401 ccgcggggcg atctccggca ggatcgcgac cgcgagcagc gcgagcaggt acgcgagcac
     2461 cacgaggtag cagcgccgct gccagcgcgc gtggtgctgg tacaccttgc cgccgaccag
     2521 cccggcgatc accaccaccc agaaggcgag cagccaccag tcgaggtaca tcatcacggc
     2581 gaacgcgagc agcgacagga tcaggccctg catcggggaa atgcgctgct cggcccgcat
     2641 gaacggctgc cagagcagca gcagaccgag gtgcgcgagc agcagcgccc gcgcccacgg
     2701 gtcggccacg ccgcggaaca ccgccacgtg cagcagggcg agcatggcca ccaccaccag
     2761 ccgccagtag cgctggctga agccccagac gacgttcgcg atgcggaaaa agaagggctc
     2821 ggtgagcgtc gccggttgca tgcggggtgt ccggcgacta gtctacaaac gaacccatgt
     2881 ctgcggccat tctcgcccgt ctggtcctgc tcgaggcgcg ccgcggcggc ctgccctggc
     2941 tcgccgcagg cagcatcgct cttgggctcg cgctagccgc atttctctcg caggtcgcgc
     3001 tgaccgagag ccgcgcgctt caggtcgcgg tcgtcgcggc gctgctgcgc gcctgcgccg
     3061 tgttcctcat cgccgcgcag gtcgcatcga gcgtgcttcg agaaatcaac gacaaggggc
     3121 tcgagctgat gctctcgctg ccgttgccgc gctcgactca ctacctcggc cggctggccg
     3181 gcttcgccgc cttgggcgtt ttgctggcag tcgtgttcgc ggccccgttg ctcgcttggg
     3241 cgcccgccgg cgcggtcgcg ctgtgggggc tgtcgctcgc ctgcgagact gcgctcatcg
     3301 ccgctgcggc gctgttcttc gccatgacgc tcgcgcagct cgtgcccgcg atcgcggcaa
     3361 ccgccggtct ttacctgctt gcgcgctcga tcgcggccat ccaggctatc gccagcggcc
     3421 cgctcgccga gccttcgctg ccgagcgagc ttgcgcgcat ggcggtgggc ggcgtcgcgc
     3481 ttctgctgcc gccgctcgat gcggtgacgc gcaccgactg gctgctctat ggggcgcccg
     3541 gcccgggtgc ctacggcgcg gcgctcgccg gcctcacgat ctatgcggcc gtgctgatcg
     3601 cggccggatt gttcgacttc cacaggagga gtgcctgaag cgcgacgcgc gcgcgcttgc
     3661 cgacgtgccg gcctggcttt gggcggcgct tgccgccacg ctcgccttgc agatcgcctg
     3721 gcgctcggcg agccctgcgc cgagccttgg agagtccgac ctgccgccgc cccccgggcc
     3781 gcaagcattg cggctcgcga gcttcggcga gccggaagcg gcggcgcgca tcgccatgct
     3841 gtacctgcag gcgttcgatc tcggcggggc caacgcggct ccgtaccaga agctcgacta
     3901 tcggcgcctc gccggctggc tgcgctcggt gctggcgctc gatccgcgca gcgcctatcc
     3961 gctgttttca gcggcccgcg tttatgcgga agttcccgat gccgagcgct gccgggtgat
     4021 gctcgagttc atctacgagg aattcctgct cgacccgaac cggcgctggc cctggctcgc
     4081 gcacgccgcg ctgctcgcca agcaccggct gaaggacctg ccgcttgcgc gccgctatgc
     4141 ggcggcgatc gaccgccgca ccactgcgca gaacgtgccg ctgtgggcca ggcaaatgga
     4201 gatcttcatc ctcgaggaca tgaacgagct cgaggcggcc aggatcatgc tcggagggct
     4261 gctcgcgagc ggcgccgtgc gcgacccgga cgaagcgcgt ttcctgcgcc accgcctcga
     4321 ggagctggag gcgaggcaac cggttaaata gtgtcatccc atcaacgcac ttgttgggaa
     4381 ttcgacgctt tcctccacaa tgtgtatgct atgttcttgt ttataaaaaa ctaccttctg
     4441 gcatgcctat tgcgatagcg gttatacctg gaagaaccat gggagaagga ttcatgaaaa
     4501 agacacaagg cttcacgctg gtcgagatcg cgatcgtgct ggtaatcatc gggctgctgc
     4561 tgggcggcat cctgaagggc caggagatga tcacgcaggc gaagatcaag aacgtgatcg
     4621 ccgatttctc gggcatctcg gcggcctacc acggctacgt cgaccgctac aagaagatcc
     4681 ctggcgacga tccgtgtgcc ggcggtgccg tgacgctggg caactgcggc accaccaccg
     4741 ggcgttggac cggcgcgacc gccggcgacg gcaacggcca ggtcgcaggc gcgtacaaca
     4801 gcaccaccgc caccgacgag tcgcgcctgt ggtgggatca cctgcgccgc gcgggcttcg
     4861 tctcgggcgt gggcgaccag cagccgttca acgcgttgag cggccagatc ggcgtgcaga
     4921 ccggaaacgc cgcttcgccg ccggtggccg cgctgggcgg cttcgccggc atcatcatct
     4981 gctcggcgaa cctgcccgac aaggtcgcga ttgccgccga cacgcagatg gacgacggcg
     5041 tgatcgacaa aggcaccgtg cgcggccagc tgcaaaccac gcccaatccc gctatcaatg
     5101 ccgccgccga cacagtggcg tacgccgaaa acggcaccaa cgtctacgtg atctgccgct
     5161 cgctgtaagc aggcgtcagc tctgacacaa gccggcctcg tgccggcttt ttttatctag
     5221 ccttgcagca atcgctgctc ggccgcgtcc aggacttcgg gagtgcccag gagcaccacg
     5281 acgtccccgg cctgcagcgc accggcctcg gcggcgctca gcttggtttt cgcgccgcgc
     5341 cggcgcaccg ctctcacctc gacgccgagc gcctcgaggc cgagctccgc gagcggcctg
     5401 ccggtcgcgt gcgcgttcgc gtcgagggtc accgcgtgca gccgcgccgc ggcttcgcca
     5461 tcgccgcgcc attcgctcgg gccgtggaac aggccgcgca acagccggta ctgctggtcg
     5521 cgcacctggc tcacgcggcg catcacgcgg ctgagcggca cgccgaccat gacgagtgta
     5581 tgcgaggcaa gcatgaggcc cgactcgaaa gcttccggca ccacttcggt ggcgccggcg
     5641 gcggtgagcc gctcgatgtc gctctcgtcg cgcgcgcgca cgatcaccgg cacgctggaa
     5701 ttgaggccgt ggatatgcgc cagcacccgc accgccgcgg cggtatcggc aaaggtcacc
     5761 accaccgccg cggcgcgcgc gatcccggcg gccatgagcg cctcgcgccg cgaactgtcg
     5821 gcgaacacga ccgcatcgcc cgcggccgcc gcctcgcgca cgcgctcggg atcgagatcg
     5881 agcgcgacgt agcgcacgcc ctcggcgtcg agcagccgcg ccaggcgctg gccgttgcgg
     5941 ccgtagccga gaatgatgac gtggcgctcc gcttccatgg attgcgacgc gacgcggtgc
     6001 agctcgagcg agcgctgcat ccactccgag cgcgagaggc gcatgacgat gcgatcgctc
     6061 gccccgatca cgaacggcgt ggcgagcatc gagaggatca ttgccgcgag cagcgcctgc
     6121 aggatttcgt cggacacgac gccggcggcg ccggcgagcg gcagcaggac gaaaccgaac
     6181 tcgccgccct gcgccagcgc gagggcgacc cgcagcgcgg tccccggcgg gctgcgaaac
     6241 gcccgagcca gcgccgcgat gaggaccaac ttgagcccga ccagcagcac gaagaacatg
     6301 agcacgagca ggaactcgcc gaagatcagg cgcaggtcga gcatcatgcc gacggtgatg
     6361 aagaacagcc ccagcagcac gtcgcggaac ggcttgatgt cctcctcgac ctggaagcgg
     6421 tactcggttt cggagatcag catgccggcg aggaaggcgc cgagcaccag cgacagcccg
     6481 gcgagaccgg tgaggaaggc gagcagcagc gtgacgagca ggacgttcag cacgaacagc
     6541 tcgttcgatc ggcgccgcgc caccgcgccg agccaggcgc gcatcagctt cggccctcca
     6601 acgatcacga tggcgagggc gagcgccgcc ttggcgagcg cgaccgcgac cgcgacgccg
     6661 atcgcatcca ccggctgccc gagcgcaggg atcagcacca gcagcggcac cacggcgagg
     6721 tcctggaaga gcagcacgcc gatcacctcg cggccatgcg cgctgtcgag ctcgccgcgc
     6781 tcggcaagca gcttcgacac gatggcggtc gaggacatcg cgatgatgcc gccgagcgcg
     6841 attccggtct gccacgcgcc gccgagcgcc atgcaggcgc cgaccgcgaa cgcgatcgtc
     6901 gccgccacct gtaacatccc caggccgaag accacgcggc gcatcgcgat cagcttcggc
     6961 aggctgaact cgaggccgat cgagaacatc aggaacacga cgccgaattc cgcgaggtgg
     7021 cgggtctcct cccggtcgga caccagcccg gccgcgtgcg ggccgagcgc gaggccggtg
     7081 gcgagatagc cgacgatcgg cggcaagcgc aggctcctgc agagcgccac cgcggcgacg
     7141 ctgcttgcaa gcagcaccag caccgactgg agcgaaacgg cgtggtccat gggctatact
     7201 ccctcgcatg ataaaggcag cgcccaagag acaaccaagc ggggaaaaaa gcggggaacc
     7261 cggcatcaaa gtgaacccgc tcgatctcgc gcggcgcgtg ctgagcatcg aggcagacgc
     7321 ggtgcgcgcg ctcgccgagc gcatcgacga gcgcttcctc gccgcggtgg cgctgatccg
     7381 cgatcgcagc ggccgcgtgg tggtgagcgg catcggcaag tccgggcaca tcgcgcgcaa
     7441 gatcgcttcc acgctgtcga gcaccggcac tccggcctac ttcgtgcatc ccgccgaggc
     7501 gagccacggc gacctcggca tgatcgagag cggcgacgtg ttcctcgcca tctcctactc
     7561 cggcgcgagc gcggagctgc tcgagatcgt gccgctggtg aagcgccgcg gcgcgaagct
     7621 gatcgtcatg accgggcgtg cggattccgc cctggcgcgc gaggcggacg tgctgctcga
     7681 tgcccgggtc gagcaggagg cctgtccgca caatctcgcg ccgacggcga gcaccaccgc
     7741 ggcgctcgcg ctcggcgacg cgctcgccgt ggcgctgctc gacgcgcgcg gcttttcggc
     7801 cgacgacttc gcgcgcagcc acccccgggg gcagctgccg cgccaggccc tcgtgcacat
     7861 cgccgacgtg atgcgcagcg ggaacgacct tcctacggtg caatgcggcg tgctcgccat
     7921 gcaggcggtg gtcgaaatgt cgcgctgccg gatggggatg accgcggtga tcgacgagcg
     7981 gcggcaggtg cgcggcatct tcaccgacgg cgacttgcgc cgttcgctcg agaagcgcgt
     8041 cgatttcggc gccacgcgcg tggaccagct gatgaccgcg gcgcccaaga ccatacggcc
     8101 cgaggcgctc gcgatcgagg ccgtgcagct gatggagagc cacaaggtga accaactgct
     8161 ggtggtgaac gagcgcggcg agctggtcgg cgcgttcaac atgcacgacc tgttccgcgc
     8221 caaagtcatt tagcgacggc atgcaataca tcgctcccga cgcgctcgaa cgggcgcggc
     8281 gtattcgact catgc
//
LOCUS       DASPXW010000426         2879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1762867, whole genome shotgun sequence.
ACCESSION   DASPXW010000426 DASPXW010000000
VERSION     DASPXW010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1762867"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1516
                     /locus_tag="VEL04_09755"
     CDS             <1..1516
                     /locus_tag="VEL04_09755"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HYT48692.1"
                     /translation="REGDYGNSAFLALESGVRVVLAPGLPQKMLGRASVKKRSFMSIV
                     SDALRRTRAPEVRNTAYYRDAIAHKDSRGEQRTSLLDVPNPAALFGADVNLEAARTRV
                     PPLAPGFKTAALPPGALFGEIAALGRVQRSATVYAEAECRVLEMRWQALRDIKNRDDL
                     WRQRIEQGYRDNQLHNHLHEHPLFAGLDPKVLQSVAARTLFETYGSFEWYLDYKSGNA
                     QAPVEPAVTAQGDYPDGLLLVIGGFGRVSSSLGHGARTLTYLRGGDHFGLDELYEAWK
                     SGHDCPYETSLSALGYLHVLRIPYSVLAEFVFPRITPPAARLSDAAARPIAQDAFLEW
                     AVDKRYINGTQTMLIDLERCVRCDDCVRACADTHGGNPRFVRQGETLGKWLVANACMH
                     CVDPVCMIGCPTGAIHRSREGGTVVINDVTCIGCGTCANSCPYDNIRLVPIRESDGRQ
                     VIDPASREPIMKATKCDLCSALPAGPSCERACAHGALRRVDIQSLISQAEAFGH"
     gene            1601..2389
                     /locus_tag="VEL04_09760"
     CDS             1601..2389
                     /locus_tag="VEL04_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48693.1"
                     /translation="MFWALGLAGWLIVALLALRAERGLRDGAVITGYALLVLMLALGM
                     FNLKKRLVALPLGTTRAWMVAHGVMGAISIPLYLQHAGGFWPGGFYERAIALCFYIVM
                     LSGIAGYVLERLLPRRLAHLENEVIYERMPSEVFALRERVEALVVKGVRDAASDTLGR
                     YYLESLHWFFRRPRFFLSHLVGSGRSARWIRGHVSALRRYLNEAERAVLDEVEQLAFR
                     KNQLDAHFALQSLLKLWLFVHVPAAVLLIGLAFWHLLVVNIYVL"
     gene            2386..>2879
                     /locus_tag="VEL04_09765"
     CDS             2386..>2879
                     /locus_tag="VEL04_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYT48694.1"
                     /translation="MSNGRKPGHDATAYERPDQPYVCGRSSLWRKSCWQGPGLDGQCG
                     GAFECIPVRSGDRWECRRPRQAGGRCSQGPLPNGSCAHRHAPCAPVSSLRRLRGRVSL
                     FAVLALVVVLILGPDPTDRTLVNPAALDAGPLSSVHAGFAREQGCSACHASHSSDAWG
                     WFSAA"
BASE COUNT          445 a          983 c          971 g          480 t
ORIGIN      
        1 gcgggagggc gactacggca attccgcttt cctggcgctc gaaagcggtg tgcgcgtggt
       61 cctggccccc ggactgccgc agaaaatgct cggccgcgcg agcgtgaaaa agcgcagctt
      121 catgagcatc gtctcggatg cgctgcggcg cactcgggct cccgaggtgc gcaacaccgc
      181 ctactatcgc gacgcgatcg cgcacaagga cagccgcggc gagcagcgca catcgctcct
      241 cgacgtaccc aatcctgcag cgctgttcgg cgccgacgtc aacctcgaag cggcccgcac
      301 tcgagtgccg ccgctcgcgc ccggattcaa gaccgcggcg ctgccgccgg gcgcgttgtt
      361 cggcgagatc gccgcgctcg gtcgcgtgca acgctcggca accgtgtacg ccgaagccga
      421 atgccgcgtg ctcgagatgc gttggcaggc gctgcgcgac atcaagaacc gcgacgacct
      481 gtggcgccag cgcatcgagc aaggctaccg cgacaaccag ctgcacaacc acctgcacga
      541 gcacccgttg ttcgccggcc tcgaccccaa ggtcctgcag agcgtggcag cacgcacgct
      601 gttcgagacc tacgggtcgt tcgagtggta cttagactac aagagcggaa acgcgcaggc
      661 gccggtcgag ccggcggtca ccgcgcaggg cgactatccc gacggtctgc tgctcgtgat
      721 cggcggcttc ggccgggtgt cgagcagcct gggccatggc gcaaggacgc tcacttacct
      781 gcgcggcggg gaccacttcg gactcgacga gctctacgag gcgtggaaga gcggtcacga
      841 ttgcccgtac gagaccagcc taagcgcgct cggctacctg cacgtgctgc gcattccgta
      901 ctcggtactt gccgagttcg tgttcccgcg catcacgccg ccggccgcgc ggctgagcga
      961 cgccgccgca cggcccatcg cgcaggacgc cttcctcgaa tgggcggtcg acaaacgcta
     1021 catcaacggc acgcagacca tgctcatcga cctggaacgc tgcgtgcgct gcgacgactg
     1081 cgtgcgcgcc tgcgccgata cgcacggcgg caatccgcgc ttcgtgcggc agggcgagac
     1141 cctcggcaag tggctggtgg cgaacgcatg catgcattgc gtcgacccgg tgtgcatgat
     1201 cggctgcccg accggggcga tccaccgctc gcgcgagggc ggcacggtcg tgatcaacga
     1261 tgtcacctgc atcggctgcg gcacctgcgc caactcctgc ccctacgaca acatccgttt
     1321 ggtaccgatc cgcgagagcg acggcaggca ggtgatcgat cccgcgtcgc gcgagccgat
     1381 catgaaagcc accaagtgcg atctttgcag cgctcttccc gccgggcctt cctgcgagcg
     1441 cgcttgcgcg cacggcgcgc tgcgccgcgt ggacatccag tcgctcatct cgcaagccga
     1501 agctttcggc cactgaacca tgaccctcct tcgcgcactg gtcctcagga acagactagg
     1561 gccccgcatg acgcctggga gggcttgggc tcagctggca gtgttctggg cgctcggact
     1621 cgccggctgg ttgatcgtcg cgctgctggc gctgcgcgcc gaacgcggcc tgcgcgacgg
     1681 cgcggtgatc accggctatg cccttctggt gctgatgcta gcgctgggga tgttcaatct
     1741 gaagaagcgg ctggtggcgc tgcccctcgg cacgacgcgc gcgtggatgg tggcccacgg
     1801 cgtgatgggc gcaataagca tcccgctcta tctgcaacat gccggcggct tctggccggg
     1861 cggattctac gagcgcgcga tcgcgctgtg tttctacatc gtgatgctgt cggggatcgc
     1921 gggatatgtc ctggagcgcc tgctgccgcg aaggctcgcg cacctggaga acgaagtcat
     1981 ctacgaaagg atgccctcgg aggtgttcgc cttgcgcgag cgcgtcgagg cgctggtggt
     2041 aaagggcgtg cgggacgccg cgtccgatac gctgggccgc tactacctcg agtcgcttca
     2101 ctggttcttc cggcggccgc gcttcttcct gagccatcta gtgggaagcg gccgcagcgc
     2161 ccgctggatc cgcgggcatg tcagtgcgct gcggcgctac ctgaacgaag ccgagcgtgc
     2221 ggtgctcgat gaggtcgagc agctggcatt ccgcaaaaat cagctcgacg cgcactttgc
     2281 gctgcagagc ctgctgaagc tctggctgtt cgtccatgtg ccggcagcgg tgctgctgat
     2341 cgggctcgcg ttctggcacc tgctcgtcgt gaacatctac gtcctgtgag caacggccgc
     2401 aagcccggcc acgatgccac cgcctacgaa agaccggacc agccatacgt ctgcgggcgc
     2461 agcagccttt ggcgcaagtc ctgctggcag ggcccggggc tggacggcca gtgcggcggg
     2521 gcattcgagt gcattccagt gcgctccggg gaccgctggg aatgccgccg ccccaggcag
     2581 gccggcggac gctgcagcca aggtccgctt cccaacggtt cctgcgcgca ccgccatgcg
     2641 ccttgcgccc cggtctcgag cctgcggcgg ctgcgcggac gggtatcgct gttcgccgtg
     2701 ctggcgctcg tggtggtgtt gatcctgggg cccgacccga ccgaccgcac gctggtgaat
     2761 ccggccgcgc tcgacgcagg acccctgtcc agcgtccatg ccggcttcgc gcgcgagcag
     2821 ggctgctctg cctgccatgc gagtcattcg agtgacgcct gggggtggtt cagcgccgc
//
LOCUS       DASPXW010000427         5408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_755950, whole genome shotgun sequence.
ACCESSION   DASPXW010000427 DASPXW010000000
VERSION     DASPXW010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5408
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755950"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VEL04_09770"
     CDS             complement(<1..217)
                     /locus_tag="VEL04_09770"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF036305.4"
                     /note="catalyzes the phosphorylation and decarboxylation
                     of oxaloacetate to form phosphoenolpyruvate using GTP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HYT48695.1"
                     /translation="MNAPAPAHADQSNAPSWVRHQALRRWVAEVVRLAKPERIVWCDG
                     SQSEYDRLCDELVEAGNFTRLNAKLRPD"
     gene            complement(424..1743)
                     /gene="mnmE"
                     /locus_tag="VEL04_09775"
     CDS             complement(424..1743)
                     /gene="mnmE"
                     /locus_tag="VEL04_09775"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HYT48696.1"
                     /translation="MPSDDVVRDTIAAIATPSGRAGIGVVRLSGAKVPQIAALLLGKL
                     PEPRRATYAVFRDTRGERIDEGIALFFPAPGSYTGEAVLELQGHGGPVVMLALLGACL
                     DAGARLAEPGEFTRRAFLEGKLDLAQAEAVADLIDAASREAARSALRSLAGEFSAAID
                     ALGAELTELRALTEAMLDFPEEEVDSLHREDAATRLARVSAALDTVIAKSRQGSLLRT
                     GIHVVLAGRPNVGKSSLLNRLAGEERAIVTAVPGTTRDALREPVQIEGVPLIVVDTAG
                     LRQSVDEVERLGIERTQRELRGADLVLAVFEAGREVARLEELAVGAARIDVYNKIDLA
                     PGFIAPAGAVAVSAKTGEGLDRLRKAILEAAGWRSTGESVFLARERHLRALETARRHL
                     AAARDVRQWEFFAEELRLAQVALGSITGEFTADDLLGEIFARFCVGK"
     gene            complement(1730..3379)
                     /gene="yidC"
                     /locus_tag="VEL04_09780"
     CDS             complement(1730..3379)
                     /gene="yidC"
                     /locus_tag="VEL04_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HYT48697.1"
                     /translation="MDTQRLILLFIFGFSLLMLWEAWEREHRPKPPPTAPVAQQGVPA
                     PAKPAVPGAPKPPAASVPAAASAVKGEIVRVTTDLLIAEIDTLGATLKRVEFTRHKDS
                     KDPAKDFVLLGPEHLYEAQSGITGDAGPNHRTAWRPQPGERALSPGIDSVAVRLTAHG
                     RDGLEVDKIYTFKRDSYLIDVRLEIRNPGAAPAAAYAYFQLTHDGKPSADANTLAQSF
                     GAQSYAGFAVFTDEHKYEKVHPGDLDKGKADHAKQANNGWLAFVQHYFVSAWLPAAGV
                     ARDYVVEKRPDGTYAGRVLVPVNVAAGASATVAVQLYTGPQEKRRLEAAAPGLDLVVD
                     YGWLAIIAWPLFWLLEKFYGLAGNWGVAIILLTVLIKILFFPLSAASYKSMAKMKLVT
                     PRLTKIREMYAHDRQKMNQAMMELYKTEKINPLGGCFPILVQIPVFIALYWVLLAAIE
                     LRHAPFILWIRDLSALDPYYVLPVLMTITMVLQTRMNPTPPDPVQAKVMQFMPFVFSI
                     FFFFFPAGLVLYWLVNNILSILQQWQIQRLFDRDKPAHAKR"
     gene            complement(3383..3595)
                     /gene="yidD"
                     /locus_tag="VEL04_09785"
     CDS             complement(3383..3595)
                     /gene="yidD"
                     /locus_tag="VEL04_09785"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HYT48698.1"
                     /translation="MIGNLMGIVLRGYQWCIRPLLPPSCRFHPSCSDYAHEALVRHGA
                     WRGSRLVARRLCRCGPWHPGGYDPVP"
     gene            complement(3592..3915)
                     /gene="rnpA"
                     /locus_tag="VEL04_09790"
     CDS             complement(3592..3915)
                     /gene="rnpA"
                     /locus_tag="VEL04_09790"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HYT48699.1"
                     /translation="MSAARRLRRRAEFERLLRQGTRRSVAGYTFFIERRDAGGPRLGM
                     LVSREHAAAAIERNRLKRCIREAFRLEQERLGPIDVLVRPPYGVKASADMTRRLRELF
                     VRLNA"
     gene            complement(3948..4082)
                     /gene="rpmH"
                     /locus_tag="VEL04_09795"
     CDS             complement(3948..4082)
                     /gene="rpmH"
                     /locus_tag="VEL04_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HYT48700.1"
                     /translation="MKRTYQPSKTRRARRHGFLVRSRTPGGRAVLRNRRARGRHRLGL
                     "
     gene            4232..>5408
                     /gene="dnaA"
                     /locus_tag="VEL04_09800"
     CDS             4232..>5408
                     /gene="dnaA"
                     /locus_tag="VEL04_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HYT48701.1"
                     /translation="MQNLWDTCLRRLEQELPAQQFNTWIRPLAPETDALSDTLVLAAP
                     NRFVLELVRERFATRIERLVAETSGRELGLRLVLARVTDSAARSPQPVQRPASEAPSL
                     LERARLNRSYSFDSFVTGKANQLARAAAIQVAENPGISYNPLFVYGGVGLGKTHLVQA
                     IGNQVADQRPQARIRYIHAEQYVTDVVRAYQQKSFDEFKRYYHSLDLLLIDDIQFFSN
                     KGRTQEEFFYAFNALVEAHKQIVITCDTYPKEIAGMEERLISRFGWGLTVAIEPPELE
                     MRVAIVLKKAEADAVGLSEDIAFFIAKHIRSNVRELEGALKKVLAFARFTERELSLDL
                     AKEALRDILNLNSRQISVEGIQKTVAEYFKLKVSDMHSKKRSRNLARPRQVAMALAKD
                     "
BASE COUNT         1013 a         1778 c         1695 g          922 t
ORIGIN      
        1 agtcagggcg cagcttcgcg ttcaggcgcg tgaagtttcc cgcctccacc aactcatcgc
       61 acaaccggtc atactcgctc tgcgaaccgt cgcaccagac gatgcgttcc ggcttggcaa
      121 gccggaccac ctccgccacc caacgccgca gcgcttgatg acgaacccaa ctcggtgcgt
      181 tggattggtc ggcatgagcc ggtgctggag cattcattgg tctcactcca aaaaggcaaa
      241 cggcccagcg ggactgggcc ggtcggatag ggactgtgtc gcatcgaatt ttagcaccca
      301 agctcgagaa agcaggtctc ccgaggggtg agccgaacag ataaaaacat gtaagtacaa
      361 tgggttatcc ggattcgaac ccgctcttgc tcgcggggtt gagcccgctg tcgcccgccg
      421 gcttcatttg ccgacgcaaa agcgcgcgaa gatctccccg agcaggtcat cggcggtgaa
      481 ttctcccgtg atgctgccga gcgcgacttg cgcgagcctc aactcctcgg cgaagaactc
      541 ccactgccgg acgtcgcgcg cggcggcgag atgtcggcgc gcagtttcga gcgcgcgaag
      601 atggcgctcg cgggcgagga ataccgattc gccggtcgag cgccagccgg ccgcctcgag
      661 aatcgcctta cgcagccgat cgaggccttc tccggtcttg gcggagaccg ccacggcacc
      721 ggcaggcgcg atgaaccccg gcgcgagatc gatcttgtta tagacgtcga tgcgcgctgc
      781 gccgacggcg agttcctcaa gccgagcgac ctctctaccg gcttcgaaaa ctgccagcac
      841 caggtcagcg cctcgcagct cgcgttgcgt gcgctcgatg cctaggcgct caacctcgtc
      901 taccgactgc cgtaaccctg ccgtatcgac cacgatgaga ggcacaccct ctatctggac
      961 cggttcacgc aatgcatcgc gcgttgtgcc cggaaccgcc gtgacgatgg cgcgctcctc
     1021 gccggcgagc cgattgagca ggctcgactt gccgacgttc ggccggccgg caagcaccac
     1081 atggatgccc gtgcgcagca ggctcccttg ccggctcttc gcgattaccg tgtcgagtgc
     1141 ggcgctgacg cgcgcaagcc gcgtcgcggc atcctcgcgg tgcagcgagt cgacttcttc
     1201 ctcgggaaaa tccagcatcg cttcggtcag ggcgcgcagc tcggtgagct cggcgccgag
     1261 cgcgtcaatc gccgcggaga attcccctgc gagggaacgc agtgccgaac gagccgcttc
     1321 gcggctcgcg gcgtcgatca gatccgccac cgcctccgct tgcgccagat cgagcttgcc
     1381 ctctagaaaa gcgcgtcggg tgaattcgcc gggctcggcg agccgggcgc cagcgtcgag
     1441 acaggcgccg agcagtgcca gcatcaccac cggtccgccg tgaccttgca gctcgagtac
     1501 cgcctcgccc gtgtaagaac ccggggcggg gaaaaaaagc gcgatccctt cgtcgatgcg
     1561 ctcgcctcgc gtgtccctga aaacagcgta cgtggcgcgg cgaggttcag gcagcttgcc
     1621 cagcagcagg gccgcgatct gcggcacctt ggcgccggag aggcgcacca cgcctatgcc
     1681 tgcacggccg ctcggggtcg cgatcgccgc gatcgtgtcg cgtactacgt catcgcttgg
     1741 catgggcagg cttgtcgcga tcgaacaagc gctggatctg ccactgctgg agaatggata
     1801 ggatgttgtt gaccagccag tagagcacca gcccggccgg gaagaaaaag aaaaagatac
     1861 tgaacacaaa aggcatgaat tgcatcacct tcgcctgcac cggatcgggc ggcgtggggt
     1921 tcatgcgcgt ctgcagcacc atcgtgatgg tcatcagcac cggcagcacg taatacggat
     1981 cgagcgccga caggtcccgg atccagagga tgaaaggcgc gtgccgcagc tcgatggccg
     2041 cgagcagcac ccagtacagc gcgatgaaca cgggtatctg aaccaggatc gggaagcagc
     2101 cgccgagcgg attgatcttc tcggtcttgt agagctccat catcgcctga ttcatcttct
     2161 gccggtcgtg ggcgtacatt tcccggatct tggtgagacg cggcgtgacc agcttcatct
     2221 ttgccatcga tttgtagctc gccgccgaga gcgggaaaaa caggatcttg atgagcaccg
     2281 tgagcaagat gatggcgacg ccccagtttc ccgccaggcc gtagaatttt tccaataacc
     2341 agaatagcgg ccaggcgatg atggcgagcc agccgtagtc cactacgaga tccaggccgg
     2401 gggcggccgc ctcgaggcga cgcttttcct gggggccggt ataaagctgg accgcgaccg
     2461 tggcactcgc ccccgcagcg acgttgacgg gaaccagcac gcgccccgca taggtgccat
     2521 ccgggcgttt ctccaccacg taatcgcgcg ccacgcctgc cgcgggcagc cacgccgaga
     2581 cgaaatagtg ctggacgaaa gcgagccagc cattgttcgc ctgcttcgcg tgatccgcct
     2641 tgcccttgtc caggtcgccg ggatggacct tctcgtactt gtgttcgtcg gtaaacaccg
     2701 cgaagccggc ataactttgg gcgccgaacg attgcgcgag cgtattggcg tcggcgctgg
     2761 gcttgccgtc atgcgtcagc tggaaatacg cgtacgccgc cgccggcgcc gcgcctggat
     2821 tgcggatctc gagcctgaca tcgatcagat aactgtcgcg cttgaaggtg tagatcttgt
     2881 cgacctcgag accgtccctg ccatgggcgg tcagccgcac cgccacgctg tcgataccgg
     2941 gcgaaagcgc gcgctcgccg ggttgcggcc gccaagcggt gcgatggttc ggccctgcat
     3001 ccccggtgat gccgctttgc gcttcataca gatgctcggg accgagaaga acgaaatcct
     3061 tcgccgggtc cttcgaatcc ttgtgccggg taaattccac ccgcttcagc gtggcgccga
     3121 gcgtgtcgat ctccgcaatc agaaggtcgg tggtcacgcg cacgatctcg cccttcaccg
     3181 ccgacgcggc tgccggtacg ctcgctgcag ggggcttcgg cgcgccgggt acggcgggtt
     3241 tggcgggcgc cggcacgcct tgctgcgcga cgggcgccgt gggcgggggc ttgggccggt
     3301 gttcgcgctc ccaagcctcc caaagcatga gcagcgagaa gccgaagatg aaaagcagga
     3361 tgagccgttg cgtatccatg gttcagggca ccgggtcgta accgccagga tgccaagggc
     3421 cgcaacgaca taggcgccgc gcaacgagcc ggctgccgcg ccacgcgcca tgacgcacga
     3481 gcgcttcatg cgcgtagtcc gagcagctcg gatggaagcg gcatgaaggc ggcagcagcg
     3541 gcctgataca ccattgatag ccgcgcagca cgattcccat gagattcccg atcatgcgtt
     3601 cagcctgacg aagagctcgc gcaggcgccg ggtcatgtcc gcggaagcct tcactccgta
     3661 gggtggccgc accagcacgt cgatcgggcc gaggcgctcc tgctccagcc ggaatgcctc
     3721 acggatgcag cgtttgaggc ggttgcgctc gattgcggct gcggcgtgct cgcgggacac
     3781 gagcatgcca agccttggcc cgcccgcgtc gcgtcgctcg atgaaaaatg tgtagcccgc
     3841 aacgcttcgc cgcgtaccct gtcgcagcag ccgctcgaat tcggctctgc gccgcagccg
     3901 ccgcgcggca ctcagcccgt ggcgcttttc gcccgcggca gcaacgacta caacccgagg
     3961 cggtgccggc cgcgcgcgcg ccgattgcgc agcaccgcgc gtccacccgg cgtgcggctg
     4021 cgaacgagaa atccatggcg tcgcgcgcgc cgcgttttgg acggctgata agtccgcttc
     4081 atcgctaccc cggtccgaaa aagggcggta ttagagcgcg cgcgacaacg cattgtcaaa
     4141 tcgccgcgcc gcgcgcgttg cacggactcg ctactgtgga taacccatcg cgaagcgggt
     4201 aaaattcagc ttcgtctagg ccaatcaaaa gatgcaaaac ctctgggata cctgcctgcg
     4261 ccggctcgag caggaactcc ccgcgcaaca attcaatact tggattcgcc ccttggcgcc
     4321 cgagacggat gcactcagcg atacgctggt gctcgctgcg ccgaaccggt tcgtgctgga
     4381 gctcgtgcgc gagcgtttcg ccacccgcat tgagcggctc gtagcagaga caagcgggcg
     4441 cgagctggga ttgcggctgg tgctcgctcg cgtgaccgac agcgcggcac gctccccgca
     4501 gccggtgcaa cgccccgcca gcgaggcgcc ttcgctgctc gaacgggcgc gcctgaaccg
     4561 ctcgtactca ttcgacagct tcgtcaccgg caaggccaat cagctcgcga gggctgcggc
     4621 tatccaggtc gccgagaatc cgggcatctc ctacaacccg ctgttcgtct acggcggggt
     4681 aggccttggc aagacgcacc tcgttcaggc aatcggcaat caagtggccg accagaggcc
     4741 gcaggcgcgc atccgctaca ttcatgccga acagtacgtg acggacgtag tgcgcgctta
     4801 ccagcagaaa tcgttcgacg agttcaagcg ctactaccac tcgctcgatc tgctgcttat
     4861 cgacgacatc cagtttttct ccaacaaggg ccgcacgcag gaggagtttt tctacgcctt
     4921 caatgcgctg gtcgaggcac acaagcagat cgtgatcacc tgcgacacct atccgaagga
     4981 aatcgctggc atggaggagc gtctcatctc gcgcttcggc tgggggctta ccgtggcgat
     5041 cgagcccccc gagctcgaga tgcgggtcgc aatcgtgctg aaaaaggccg aggcggacgc
     5101 cgtcggcctt tccgaggaca tcgcattttt cattgccaag catatccgct cgaacgtgcg
     5161 cgagctcgag ggcgcactga agaaagtcct cgccttcgcg cgcttcacgg aacgcgagct
     5221 ctcgctcgac ctcgcaaagg aagcattgcg cgacatcctc aatctcaaca gccgtcagat
     5281 cagcgttgaa ggaatccaga aaaccgtcgc cgagtacttc aagctcaagg tatccgacat
     5341 gcactccaag aagcgcagcc gcaacctggc gcggccgcgc caagttgcca tggcgctcgc
     5401 caaggacc
//
LOCUS       DASPXW010000428         4022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1637874, whole genome shotgun sequence.
ACCESSION   DASPXW010000428 DASPXW010000000
VERSION     DASPXW010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1637874"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VEL04_09805"
     CDS             <1..219
                     /locus_tag="VEL04_09805"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015421006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HYT48702.1"
                     /translation="VLMEHTSKDGASKILKRCTLPITGKAVVNMIITDLCVFEVEPGK
                     GLALKELHAGVTLEDVSKKTAAQFRVAL"
     gene            266..1912
                     /locus_tag="VEL04_09810"
     CDS             266..1912
                     /locus_tag="VEL04_09810"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HYT48703.1"
                     /translation="MTKYVFVTGGVVSSLGKGIAAASLAAILESRGIRVTNIKLDPYI
                     NVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFQSSKMRRWNNFTTGQIYESVIKKE
                     RRGDYLGGTVQVIPHITDEIKLFIKRGAGESDVAIVEVGGTVGDIESLPFLEAIRQMG
                     LEMGRNNVCYIHLTLVPYIASAGEIKTKPTQHSVKELRQIGIQADALLCRTVRPLPED
                     ERRKIALFTNVQKEAVISARDVDSIYKIPMMLHEQMLDEIVCHKLGILAKAADLSSWT
                     RLVQAQDHPEHEVDIAFVGKYVDLQDSYKSLNEALKHAGIHTSSKVNIHYFDSEELEK
                     SGSAELEKMDAILVPGGFGKRGTEGKMRAIRYAREHGVPYLGICLGMQLATIEFARNV
                     CGLANANSTEFDADTPHPVVALITEWLDRTGRIEQRSEKSDLGGTMRLGAQKCAVEAA
                     TLASSIYGLEVNERHRHRYEVNNIYVPQLEAKGYKVSARTPSEKLPEMMEIPGHPFFV
                     GVQFHPEFTSTPRNGHPLFKAFVAAALRHRAGSKLRAVAA"
     gene            1909..2754
                     /gene="kdsA"
                     /locus_tag="VEL04_09815"
     CDS             1909..2754
                     /gene="kdsA"
                     /locus_tag="VEL04_09815"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HYT48704.1"
                     /translation="MNLCGFDVGLERPLFLIAGPCVVESRELQVETAGRLKEICKPLG
                     IPLIFKSSYDKANRSSGKSFRGLGMEKGLEILTHVKKAIGIPVLTDVHTEEEVRPVAA
                     VVDVLQTPAFLCRQTDFIRAAASAGRPVNIKKGQFLSPQEMRNVVDKAKEASGADNIL
                     VCERGFSFGYGNLVSDMRSLAIMRATGCPVVFDATHSVQLPGGQGSASGGEREFIPVL
                     ARAAVASGIAGLFMETHPNPDQALSDGPNAWPLGLMRGLLETLVEIDRMVKKNGFAEQ
                     KVKQT"
     gene            2751..>4022
                     /gene="eno"
                     /locus_tag="VEL04_09820"
     CDS             2751..>4022
                     /gene="eno"
                     /locus_tag="VEL04_09820"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HYT48705.1"
                     /translation="MSSIVDVVAREILDSRGNPTVEADVLLESGVMGRAAVPSGASTG
                     SREAIELRDGDKGRYGGKGVLKAVEFVNTEISEAIVGLDATEQAFIDKTLIELDGSEN
                     KSRVGANSLLAVSMAVAKAAAEESGLPLYRYFGGSGAMQMPVPLMNLINGGAHANNRL
                     DLQEFMIVPVGAQSFREALRCGSEVFQSLKKLIDSKGMSTAVGDEGGFAPDLPTHAAA
                     LDLLVESIGRAGYSAGQDVMLALDCAASEFCRDGRYVLESEKKTLNPGEFVDYLADLA
                     KRYPIVSIEDGMAENDWEGWKLLTAKLGRKLQIVGDDVFVTSTRLLREGIRQGVANSI
                     LIKVNQIGTLTETFAAIEVAKRAAYGTVISHRSGETEDTTIADIAVGSNALQIKTGSL
                     SRSDRVAKYNQLLRIEEDLGDSVSYPGREAFA"
BASE COUNT          780 a         1280 c         1298 g          664 t
ORIGIN      
        1 gtgctgatgg agcacacttc caaggacggc gcctcgaaga tcctcaagcg ctgcacgctg
       61 ccgatcaccg ggaaggcggt ggtcaacatg atcatcaccg acctgtgcgt gttcgaggtc
      121 gagcccggca aggggctggc gctgaaagaa ctgcacgcgg gcgtcacgct cgaagatgtg
      181 agcaaaaaaa ccgctgcgca gtttcgcgtc gcgctgtgat ggtgtttttc gtgtaaactc
      241 ctccatccca ccacgcgatt aggtgatgac caagtatgtc ttcgtaaccg gtggtgtagt
      301 ttcctctctg gggaaaggca tcgccgccgc ctccctcgcc gcgatcctcg aatcgcgcgg
      361 tatccgagtc accaacatca agctggaccc gtacatcaac gtcgatccgg gcaccatgag
      421 cccgtttcag cacggcgagg tgttcgtcac cgaggacggc gcggaaaccg acctcgatct
      481 cggccactac gagcgcttcc agagctcgaa gatgcgccgc tggaacaact tcaccaccgg
      541 ccagatctac gagtcggtga tcaagaagga acgccgcggc gactacttgg gcggcacggt
      601 gcaggtgatc ccgcacatca ccgacgagat caagctgttc atcaagcgcg gcgccggcga
      661 atccgacgtc gcgatcgtgg aagtgggcgg caccgtcggc gacatcgagt cgctgccttt
      721 cctcgaagcg atccggcaga tggggctcga gatgggccgt aacaacgtct gctacatcca
      781 cctcacgctg gtgccctaca tcgcctccgc cggggagatc aagaccaagc cgacccagca
      841 ctcggtgaag gagctgcgcc agatcggcat ccaggccgat gcgctgctgt gccgcacggt
      901 gcggccgctg cccgaggacg agcgccgcaa gatcgcgctg ttcaccaacg tgcagaagga
      961 ggcggtgatc tcggccaggg acgtcgactc gatctacaag atcccgatga tgctgcacga
     1021 gcagatgctc gacgagatcg tgtgccacaa gctcggcatc ctggcgaagg ccgcggactt
     1081 gtcgtcgtgg acccggctgg tgcaggcgca ggaccatccc gagcacgagg tcgatatcgc
     1141 cttcgtcggc aagtacgtcg acctgcagga ttcctacaag tcgctgaacg aggcgctgaa
     1201 gcacgccggc atccacacca gctcgaaggt caacatccat tacttcgact ccgaggagct
     1261 ggagaagagc ggcagcgcgg agctcgagaa gatggatgcg atcctggtgc ccggcggctt
     1321 cggcaagcgc ggcaccgagg gcaagatgcg cgccatccgc tacgcgcgcg agcatggcgt
     1381 gccttacctc ggcatctgcc tcggcatgca gctcgcgacc atcgagttcg cgcgcaacgt
     1441 ctgcgggctc gccaatgcca acagcaccga gttcgatgcc gacacgccgc acccggtggt
     1501 ggcgctgatc accgaatggc tcgaccgcac cggccgcatc gagcagcgct cggaaaaatc
     1561 ggacctcggc ggcaccatgc gcctgggagc gcagaaatgc gccgtcgagg cggcaacact
     1621 cgcttcttca atttatggtt tagaggtaaa tgaaaggcat cgtcaccgct acgaggtcaa
     1681 caacatctac gttccccaac tcgaggcgaa gggctacaag gtgtccgccc gcacgccgag
     1741 cgagaagctg cccgagatga tggagattcc cggccacccg ttcttcgtcg gcgtgcagtt
     1801 ccaccccgag ttcacctcga cgccgcgcaa cggccatccg ctgttcaagg ccttcgtcgc
     1861 cgccgcgctg cggcaccgcg ccggttccaa gctgcgggcc gtcgcggcgt gaacctttgc
     1921 ggcttcgatg tcgggctaga gcggccgctc ttcctgatcg cgggcccctg cgtggtcgaa
     1981 tcgcgcgagc tgcaggtgga gaccgcggga cgcctgaagg agatctgcaa gcccctcggc
     2041 atcccgctga tcttcaaatc ctcctacgat aaggcgaacc gcagctcggg aaagtctttt
     2101 cggggcctcg gcatggagaa gggtttggaa attcttactc atgtaaaaaa agcaatcgga
     2161 attcccgtgc tgaccgacgt gcacaccgag gaggaagtgc ggccggtggc cgctgtcgtc
     2221 gacgtgctgc agacgccggc gtttctctgc cggcaaaccg atttcatccg cgccgcggct
     2281 tcggcgggcc ggccggtcaa cataaagaag ggccagtttc tgtcgcctca ggagatgagg
     2341 aacgtggtcg acaaggcaaa agaggcaagc ggcgcggaca acatcctggt ctgcgagcgc
     2401 ggtttctcgt tcggctacgg caacctggtg tcggacatgc gctcgctcgc catcatgcgc
     2461 gcgaccggat gcccggtggt gttcgacgcc acgcactcgg tgcaattgcc cggcgggcag
     2521 ggctccgctt cgggcggtga gcgggaattc atcccggtgc tggcgcgcgc cgccgtggcg
     2581 agcggcatcg ctggcctgtt catggagacg catcccaatc ccgaccaggc gctctccgac
     2641 ggcccgaatg cctggccgct cggcctgatg cgggggctgc tcgagaccct ggtcgagatc
     2701 gatcgcatgg tgaaaaagaa cggctttgcc gaacagaaag tgaaacagac atgagctcca
     2761 tagtggacgt cgtcgcgcgg gagatcctcg actcgcgcgg caatccgacg gtcgaagccg
     2821 acgtgctgct cgagtccggc gtgatggggc gcgcagcggt gccctcgggc gcctcgaccg
     2881 gcagccgcga ggcgatcgag ctgcgcgacg gcgacaaggg ccgctacggc ggcaagggcg
     2941 tgctcaaggc ggtggagttc gtcaacaccg agatctccga ggcgatcgtc gggctcgacg
     3001 ccaccgagca ggcgttcatc gacaagaccc tgatcgagct cgacggcagc gagaacaagt
     3061 cgcgcgtcgg cgcgaactcg ctgctcgcgg tatcgatggc ggtagcgaag gccgccgccg
     3121 aggaatcggg gctgcccctg taccgctatt tcggcggctc gggtgcgatg cagatgccgg
     3181 tgccgctgat gaacctgatc aacggcggcg cccacgccaa caaccgcctc gacctgcagg
     3241 aattcatgat cgtgccggtc ggcgcgcagt ccttccggga agcattgcgc tgcggatcag
     3301 aggtttttca atctttaaaa aaactgattg attcgaaagg catgtccacg gcggtcggcg
     3361 acgagggcgg cttcgcgccg gatctgccca cccacgccgc ggcgctggac ctgctcgtcg
     3421 agtcgatcgg cagggcgggg tacagcgccg ggcaggacgt gatgctcgcc ctcgactgcg
     3481 cagcctcgga gttctgtcgc gacggccgct atgtcctcga gtctgaaaag aagacgctta
     3541 atccggggga attcgttgac tacctcgccg acctggcgaa gcgctacccg atcgtgtcga
     3601 tcgaggacgg catggccgag aacgactggg aaggctggaa gctgctgaca gccaagctcg
     3661 gcaggaagct ccagatcgtc ggtgacgatg tcttcgtcac cagcacgcgc cttttgcgcg
     3721 agggcatccg ccagggcgtg gccaactcga tcctcatcaa agtgaaccag atcggcactc
     3781 tcaccgagac ctttgccgcg atcgaggtcg ccaaacgcgc ggcctacggc acggtgatct
     3841 cgcaccgctc gggcgagacc gaggacacca ccatcgccga catcgcggtc ggcagcaacg
     3901 cgctgcagat caagaccggg tcgctgtcgc gctccgaccg ggtcgcgaaa tacaaccagc
     3961 tgctgcgcat cgaggaagac ctcggcgact cggtcagcta ccccgggcgg gaagcgttcg
     4021 cg
//
LOCUS       DASPXW010000429         3194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_879972, whole genome shotgun sequence.
ACCESSION   DASPXW010000429 DASPXW010000000
VERSION     DASPXW010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879972"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="VEL04_09825"
     CDS             <1..527
                     /locus_tag="VEL04_09825"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYT48706.1"
                     /translation="QLLREADRHRDAYDMLGQALQKDPDQPELLYDYALTAEKLERFD
                     VLESNLRKLIQVRPDHAHAYNALGYSFAERNTRLPEARKLIEKALELAPEDFFIIDSL
                     GWVQYREGDLKGAADTLRRAYGGRPDAEIGAHLGEVLWVMGRRDEASRIWQEALKTAP
                     DNETLLKTIKRLRP"
     gene            524..1054
                     /gene="lolB"
                     /locus_tag="VEL04_09830"
     CDS             524..1054
                     /gene="lolB"
                     /locus_tag="VEL04_09830"
                     /inference="COORDINATES: protein motif:HMM:NF015512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HYT48707.1"
                     /translation="MTRAWLAALALLGGCAQVEIKPPTAGALEFELAGRIAARYGTES
                     FSGNVAWRHARGGDEMLISTPFGQGVARIVREGEAVLLATAEPREYRAVDAESLTERV
                     LGFRVPLLGLADWVRARASEGAPARAEYSPDGKLAKLEQNGWRVEYQEYEGTRPARLR
                     LLYQGIELRLAVTKWN"
     gene            1045..1869
                     /gene="ispE"
                     /locus_tag="VEL04_09835"
     CDS             1045..1869
                     /gene="ispE"
                     /locus_tag="VEL04_09835"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HYT48708.1"
                     /translation="MELSWHPAPGKLNLFLHVLGRRADGYHELQTVFRLIDRCDRVGI
                     SLRNDAEIRFSGMFGEDNLCVRAARLLQKETGSKLGADLRLEKQLPVGGGLGGGSSDA
                     GTVLLVLNRLWKLFLPREKLMELALQLGADVPVFVYGRNALGEGIGERLTALELPRAW
                     YVVLAPQVSVSTKETFQSALTRPAKRLKIPPFFSGQGANDLEPLVTARYPEVAAQLAW
                     LRKQRPQARMTGSGACVFAEFEKEADARATQSQVPGGMSGFVAQGLERHPLYDWAD"
     gene            1911..1987
                     /locus_tag="VEL04_09840"
     tRNA            1911..1987
                     /locus_tag="VEL04_09840"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1945..1947,aa:Gln,seq:ttg)
     gene            1999..2958
                     /locus_tag="VEL04_09845"
     CDS             1999..2958
                     /locus_tag="VEL04_09845"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HYT48709.1"
                     /translation="MTNAPLDRLMVFTGNANPRLAQEVAKKLNLSIGRAIVGKFSDGE
                     VMVELLENVRGKDVFVLQSTCAPTNDNLMELLVMVDALKRSSAARVTAAIPYFGYARQ
                     DRRPRSARVAISAKVVANMLTSVGVARVLTMDLHADQIQGFFDLPVDNIYAAPVLLGD
                     VWKQRYEDLIVVSPDVGGVVRARALAKRLESDLAIIDKRRPKANVSEVMNVIGEVKGR
                     TCVIMDDMVDTAGTLVKAAQVLKEEGATKVVAYCTHPVLSGGAVERIAKSDLDELVVT
                     DTIPLGDDAKACRKIRSLSVAGLIAETILRIYTEESVSSLFIE"
     gene            2979..>3194
                     /locus_tag="VEL04_09850"
     CDS             2979..>3194
                     /locus_tag="VEL04_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HYT48710.1"
                     /translation="MAIEITARKRDTQGTGPSRRLRKTGKVPGIVYGGEHGAVNIELD
                     HRNLYINLRNEKFHASILTLDLGGAKQQ"
BASE COUNT          569 a         1027 c         1092 g          506 t
ORIGIN      
        1 cgcagctgct gcgcgaggcg gaccggcatc gcgacgccta cgacatgctc ggccaggcgc
       61 tgcagaagga tcccgaccag ccggagctgc tctacgacta cgcgctcacg gccgagaagc
      121 tcgagcgctt cgacgtgctc gaatcgaacc tgaggaagct gatccaggtg cggcccgatc
      181 acgcgcatgc ctacaacgct ctcggctatt cgttcgccga gcgcaacacc cggctgcccg
      241 aggcgaggaa gctgatcgag aaggcgctcg aactcgcgcc cgaggacttc ttcatcatcg
      301 acagcctggg ctgggtgcag tatcgcgagg gcgacctcaa gggggcggcc gatacgctgc
      361 gccgcgccta cggcggacgg cccgacgccg agatcggcgc gcatctcggc gaggtgctgt
      421 gggtgatggg ccggcgcgac gaagccagcc gcatctggca ggaagcgctg aagaccgcgc
      481 cggacaacga gacccttctc aagacgatca aacgattgcg gccgtgacgc gggcctggct
      541 cgccgcgctg gcgcttctcg gcggctgcgc acaggtcgag atcaagccgc ccaccgcagg
      601 cgccctcgag ttcgagctcg ccgggcgcat cgcggcgcgc tacggcaccg agtcgttctc
      661 cggcaacgtc gcctggcgcc atgcccgcgg cggcgacgaa atgctgatca gcacgccgtt
      721 cggccagggc gtggcgcgca tcgtgcgcga gggcgaggcc gtgttgctcg ccaccgccga
      781 gccgcgcgaa taccgcgccg tcgacgccga gtcgctcacc gagcgggtgc tcggctttcg
      841 cgttccgctg ctcgggctcg cagactgggt gcgcgcccgc gcctccgagg gcgcgccggc
      901 gcgcgccgaa tattcgcccg acggcaagct ggcgaaactc gagcagaacg gctggagggt
      961 cgagtaccag gaatacgagg gaacgcggcc ggcgcggctg cggctgctct accagggaat
     1021 cgagctgcgc ctcgccgtga ccaagtggaa ctgagctggc atccggcgcc cggcaagctg
     1081 aaccttttcc tgcacgtcct cggccggcgc gccgacggct accacgagct gcagaccgtg
     1141 ttccgcctca tcgaccgctg cgaccgcgtc ggtatttccc tgagaaatga tgcagagatt
     1201 cgcttctccg ggatgttcgg cgaagacaac ctgtgcgtgc gcgccgcgcg gcttctccag
     1261 aaggaaaccg gttcaaagct tggagcagat ctgagactcg agaaacagtt gcctgtcggc
     1321 ggcggcctgg gcggcggctc ctcggacgcc ggcaccgtgc ttttggtttt gaatcggctc
     1381 tggaagctct ttctcccaag ggaaaaattg atggagctcg cgttgcaact cggcgcggat
     1441 gtgcccgttt tcgtctatgg ccgcaacgcc ctgggcgaag gcatcggcga gcgcctgacc
     1501 gcgctcgagc tgccgcgcgc ctggtacgtc gtgttggcgc cgcaagtctc ggtttccacg
     1561 aaggaaacat tccaaagcgc gttgacacgc cctgcgaaac gactcaaaat accgcccttt
     1621 ttctcggggc agggagcgaa cgatctcgag ccgctcgtca ccgcccgcta ccccgaggtg
     1681 gcagcgcagc ttgcgtggct gagaaagcag cgcccgcagg cgcgcatgac gggctccgga
     1741 gcgtgcgtgt tcgccgaatt cgagaaagaa gccgacgcgc gcgccactca gtcccaggtg
     1801 ccggggggaa tgagcggatt cgtggcgcag gggcttgagc gccatccgct gtatgactgg
     1861 gcggattgaa ttgaactcag ggcgaagatg ctccgccccg aattcacaac tggggagtcg
     1921 ccaagctggt taaggcaccg gattttgatt ccggcattcg tgggttcgaa tcctacctcc
     1981 ccagccacac gacatgacat gaccaacgcg cccctcgacc ggctgatggt gttcacgggc
     2041 aacgccaatc cgcgcctggc gcaggaggtg gcgaagaagc tcaatctgtc gatcggccgc
     2101 gccatcgtcg gcaagttttc cgacggcgag gtgatggtcg agctgctgga gaacgtgcgc
     2161 ggcaaggacg tgttcgtgct gcagtcgacc tgcgcgccga ccaatgacaa cctgatggag
     2221 ctgctggtga tggtcgatgc gctgaagcgc tcatccgccg cgcgcgtcac cgcggcgatc
     2281 ccgtacttcg gctacgcgcg ccaggaccgc aggccgcgct cggcgcgggt ggcgatcagc
     2341 gccaaggtgg tggcgaacat gctgacctcc gtgggcgtgg cgcgcgtgct gacgatggac
     2401 ctgcatgccg accagatcca ggggttcttc gacctgccgg tcgacaacat ctacgccgcg
     2461 ccggtgctgc tgggcgacgt gtggaagcag cgctacgagg acttgatcgt cgtctccccg
     2521 gacgtcggtg gcgtggtgcg ggcgagggcg ctcgcgaagc ggctggagtc ggacctcgcg
     2581 atcatcgaca agcgccggcc gaaggcgaat gtctccgagg tgatgaacgt gatcggcgag
     2641 gtcaagggac gcacctgcgt gatcatggac gacatggtcg atacggcggg cacgctggtg
     2701 aaggcggctc aggtgctgaa ggaggagggc gcgaccaagg tggtcgccta ttgcacgcac
     2761 ccggtgctct ccggcggcgc ggtcgagcgc atcgcgaaat cggacctcga cgagctggtg
     2821 gtgaccgata ccatcccgct cggcgacgac gccaaggcgt gcaggaagat ccgctcgctg
     2881 tcggtcgccg gcctgatcgc cgagacgatc ctgaggattt acaccgagga gtcggtgagc
     2941 tccttgttca tcgaataatt ttttaccgga gcaacgcgat ggcaatcgaa atcacggcgc
     3001 gcaagcgcga cacccagggc acgggtccga gccggcgcct gcgcaagacc ggcaaggtgc
     3061 cgggcatcgt ctacggcggc gagcacggcg cggtcaacat cgagctcgac cacaggaacc
     3121 tgtacatcaa cctgcgcaac gagaagttcc acgcctcgat cctgacgctc gatctcggcg
     3181 gcgccaagca gcag
//
LOCUS       DASPXW010000430         2308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1006995, whole genome shotgun sequence.
ACCESSION   DASPXW010000430 DASPXW010000000
VERSION     DASPXW010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006995"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VEL04_09855"
     CDS             complement(<1..500)
                     /locus_tag="VEL04_09855"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYT48711.1"
                     /translation="MKTLVAAIGFALAAAPASAEYASVNGLKMYYEIQGAGKPLVLLH
                     GGVCTIEVCLGKIRAPLAKSWKTIAIEQQGHGRTADADRPLSYEQMAEDTAALLRQLK
                     VRNADVFGYSMGGSIALVIAMRHPDLVRKVVVLGTAYNNDGLILGLVENFKNLKPEEI
                     PQQFRDA"
     gene            complement(497..937)
                     /locus_tag="VEL04_09860"
     CDS             complement(497..937)
                     /locus_tag="VEL04_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYT48712.1"
                     /translation="MKISPFLWFDNQAEEAAKFYAAVFKKAKVGKPVKGPDGKVMVVP
                     FEIEGVEFTALNGGPQFKFNESVSFVVHCKDQDEVDYYWDKLTSAGGKESMCGWLKDK
                     FGLSWQIVPAILPELMRGPKAGKVMQTFMQMRKFDIAALKRAAA"
     gene            complement(897..2120)
                     /locus_tag="VEL04_09865"
     CDS             complement(897..2120)
                     /locus_tag="VEL04_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYT48713.1"
                     /translation="MSNRYGWVMVALGAFMTCIAIGAMFSLAVFLQPITEATGWSHTA
                     VSSAMTIGFLAMGAAGFGWGALSDRIGPRSVVLAGAVLLGLGLFLASRAQSSLQFQLA
                     FGLLVGIAVGSFFTPMIAAVMGWMAEHRGLAVSLVSVGVGVAPMTMSPFAAWLITQMD
                     WRSAQVVLAVLVWATLVPAALFVRRPPAAQAAEQGAAEGPRMSLRDAFVSMPFIVLAL
                     TFFACCAAHAGPIFHTVTYAMTCGLPTMAAVTIYSVEGFAGLGGRLALGVLADRLGAK
                     RVLIAGLLIQALAAGAFVFASRLGEFYAVAATFGFAYGGVMPLYAVLARAYFGQRILG
                     AVFGAAAMVSSLGMALGPAVGGWIFDRFGAYAWLYIGSFAVGIGAAAIALAFPPVNSR
                     RSHENQSVPLVRQPG"
BASE COUNT          407 a          806 c          749 g          346 t
ORIGIN      
        1 gcgtcgcgga actgctgcgg gatctcctcg ggcttcaggt tcttgaagtt ctccaccagg
       61 ccgaggatca ggccgtcgtt gttgtaggca gtgccgagga cgacgacctt gcgcaccagg
      121 tcgggatggc gcatggcgat caccagcgcg atcgaacctc ccatgctgta gccgaagacg
      181 tcggcgttcc gcaccttgag ctgccgcagc agcgccgcgg tgtcttccgc catctgctcg
      241 taggagagcg gccggtcggc gtcggcggtg cgcccgtgcc cctgctgctc gatggcgatg
      301 gtcttccagc tcttcgccag cggtgcccgg atcttgccca ggcagacctc gatggtgcac
      361 acgccgccgt gcagcagcac cagcggcttt ccggcgccct ggatctcgta atacatcttg
      421 aggccgttga ccgaggcgta ttcggccgag gccggggcag cggcaagcgc gaaaccgatg
      481 gctgcgacga gcgttttcat gccgccgccc gcttcagcgc ggcgatgtcg aacttcctca
      541 tctgcatgaa ggtctgcatc accttgcccg ccttcggccc ccgcatcagc tccggaagga
      601 tagccggcac gatctgccag gacaggccga acttgtcctt cagccagccg cacatcgatt
      661 ccttgccgcc ggccgaggtg agcttgtccc agtagtagtc gacctcgtcc tggtccttgc
      721 agtgcacgac gaacgagacc gactcgttga acttgaactg cggcccgccg ttcagcgcgg
      781 tgaactccac gccctcgatc tcgaacggca ccaccatgac cttgccgtcg ggccctttca
      841 ccggcttgcc taccttcgcc ttcttgaaca cggcggcgta gaacttcgcc gcctcctcag
      901 cctggttgtc gaaccagagg aacggactga ttttcatgcg atctccttga attgaccggc
      961 gggaaggcga gtgcgatcgc cgccgcgccg attcccaccg cgaacgaacc gatgtatagc
     1021 caggcatagg cgccgaagcg gtcgaagatc catccgccca ccgccggccc gagcgccatc
     1081 ccgaggctgg acaccatggc ggcggcgccg aacaccgcac cgaggatgcg ttgcccgaag
     1141 tacgcgcgcg cgagcaccgc gtagagcggc atcacgccgc cgtaggcgaa gccgaacgtg
     1201 gcggcgacgg cgtagaactc gccgagccgc gaggcaaaga cgaacgcgcc cgcggcgagc
     1261 gcctggatca gcagcccggc gatgagcacg cgcttcgcgc caaggcggtc ggcgagcacg
     1321 ccaagcgcga ggcggccgcc gagcccggcg aagccttcga cgctgtagat ggtcacggcg
     1381 gccattgtcg gcaggccgca ggtcatggcg taggtgaccg tgtggaagat cggcccggcg
     1441 tgcgccgcgc agcaggcgaa gaaagtgagc gcaaggacga tgaacggcat tgagacgaac
     1501 gcgtcgcgca ggctcatccg gggtccttcg gctgcgccct gctccgccgc ctgcgctgcc
     1561 ggcggccggc gcacgaagag cgccgccgga acgagtgtcg cccacacgag cacggcaagc
     1621 acgacctgcg cgctgcgcca gtccatctgc gtgatgagcc acgcggcgaa cggcgacatc
     1681 gtcatcggcg cgaccccgac cccgaccgag accagcgata ccgcgagacc gcgatgctcg
     1741 gccatccagc ccatgaccgc ggcgatcatc ggcgtgaaga agctgcccac ggcgatgccg
     1801 accagcaggc cgaacgcaag ctggaactgg agcgagctct gcgcgcggct cgcgaggaac
     1861 aggccgagcc cgaggagcac cgcgccggcc agcacgacgc tgcgcggtcc gatgcggtcg
     1921 ctcagcgcgc cccagccgaa gcccgccgcg cccatcgcca ggaagccgat cgtcatggcg
     1981 ctcgataccg cggtgtgcga ccagccggtc gcttcggtga tcggctgcag gaacaccgcg
     2041 agcgagaaca tggcgccgat cgcgatgcac gtcatgaagg cgccgagcgc caccatcacc
     2101 cagccgtaac ggttgctcat ttctttttct tccttttcga ccaagttatc cctccaggga
     2161 ctttgtcaga gcatccatca gcgcgccggt gccgcgctcg cggctgccag catcgtcgta
     2221 gccatcgtga caaggcgaaa ggcgcctccc ggccggggat ccgctacgca ggacgggacg
     2281 atgaagccct tcgggccgaa ccagcgcg
//
LOCUS       DASPXW010000431         3813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_502496, whole genome shotgun sequence.
ACCESSION   DASPXW010000431 DASPXW010000000
VERSION     DASPXW010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502496"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VEL04_09870"
     CDS             complement(<1..405)
                     /locus_tag="VEL04_09870"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HYT48714.1"
                     /translation="MLRVGIVGWRGMVGSVLVQRMREERDFDHIEPVFFSTSQAGGKG
                     PAIGEQTCPVQDAMHIATLKALPVLISCQGSDYTKDVYPRLRGAGWKGYWIDAASALR
                     MKDDAVIVLDPVNMPVIKRALHAGMRNYIGGNC"
     gene            complement(416..1468)
                     /gene="leuB"
                     /locus_tag="VEL04_09875"
     CDS             complement(416..1468)
                     /gene="leuB"
                     /locus_tag="VEL04_09875"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014725192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HYT48715.1"
                     /translation="MRVAVLPGDGIGPEIIAQATKVLQRLNLGLEMQQAPVGGAGYES
                     AGDPLPPATLKIAKDADAILFGAVGDPRYDSLPRAKRPEQAILGLRKELGLFANLRPA
                     RLHPELAAASPLKVEVVCGLDILIVRELTGDIYFGAPRGIREVNGAREGFDTMRYSEP
                     EIRRIAKVAFEAARKRARRVCSVDKANVLETSQLWREVVIQESKNYGDVELSHMYVDN
                     CAMQLVRNPKQFDVIVTGNLFGDILSDEASMLTGSIGMLPSAALDARGRGLYEPIHGS
                     APDIAGKGIANPLAAILSAAMMLRFSLRQEKAAERVEAAVSKVLRAGLRTADICTAGM
                     RQVNTLEMGDAVAAAL"
     gene            complement(1470..2063)
                     /gene="leuD"
                     /locus_tag="VEL04_09880"
     CDS             complement(1470..2063)
                     /gene="leuD"
                     /locus_tag="VEL04_09880"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HYT48716.1"
                     /translation="MEKFTVLNGLVAPLDRANVDTDQIIAKEHLKSIKRTGFSEALFG
                     AWRKDPEFVLNQPRYRGAAILLARKNFGCGSSREHAPWALMEWGFRCIVAPSFADIFF
                     NNCFKNGLLPVALTEAEVDQLFYECVAFPGFRLIVNLENQSVSQVDASRTMQFEVDPF
                     RKYCLLNGLDEIGLTLRHADEIRAFEARRKAQFPWYF"
     gene            complement(2068..3483)
                     /gene="leuC"
                     /locus_tag="VEL04_09885"
     CDS             complement(2068..3483)
                     /gene="leuC"
                     /locus_tag="VEL04_09885"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HYT48717.1"
                     /translation="MGQTLYDKLWDSHLVHLGHDGTALLYIDRHLVHEVTSPQAFEGL
                     KVAGRKPWRIDSVVATADHNTPTTDWNQGMQGVRDPFAKLQIQTLDANMREFGAKVYF
                     PFLDARQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFACLAHGIGTSEVEHVLATQ
                     CLVTQKMRNMRVSVEGPLASGVTAKDVVLAIIGRIGTAGGTGHAIEFAGSTIRALSME
                     GRMTVCNMSIEAGARAGMVACDQTTVAYVKGRPFAPKGADWERAVAYWRTLKSDPDAK
                     FDREVMLDAREIKPHVTWGTSPEMVVSVDDVVPDPDREKDAVRREGMERALVYMGLKP
                     RTRITDIKLDKIFIGSCTNSRIEDLRAAAAVVKGRRIAPNVRLALVVPGSGVVKSQAE
                     REGLDRIFRDAGFEWREPGCSMCLGMNPDRLGSGERCASTSNRNFEGRQGEGGRTHLV
                     SPAMAAAAAIAGHFVDIRRWG"
     gene            complement(3515..>3813)
                     /locus_tag="VEL04_09890"
     CDS             complement(3515..>3813)
                     /locus_tag="VEL04_09890"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYT48718.1"
                     /translation="WAAAELWILVAAQLLHAASFGAFHAASVAAVHRVFPHAAQARGQ
                     TLFSSLSYGAGGAAGALLAGWAWEASGPGPAFSVSALAALAGAYFAHRLKRAGL"
BASE COUNT          623 a         1286 c         1250 g          654 t
ORIGIN      
        1 gcaattgccg ccgatgtaat tcctcatgcc ggcgtgcaga gcccgcttga tgaccggcat
       61 gttgacggga tcgagcacga tcaccgcgtc atccttcatg cgcaacgcgc tcgcggcgtc
      121 gatccagtag cccttccagc cggcgccgcg cagcctggga taaacgtcct tggtgtagtc
      181 gctgccctgg caggaaatga gcaccggcag ggccttgagc gtcgcgatgt gcatcgcatc
      241 ctgcaccggg caggtctgct cgccgatcgc gggacccttg ccgccggcct gcgaggtcga
      301 gaagaatacc ggctcgatgt ggtcgaagtc gcgctcctcg cgcatgcgct gcacgagcac
      361 cgagcccacc atgcctcgcc agccgacgat gccgactctc aacatgactc gtagtttata
      421 gggcggcggc taccgcgtcg cccatttcca gagtgttgac ctgcctcatt ccggccgtgc
      481 aaatgtccgc cgtgcgcaag cccgcgcgca gcaccttgct taccgccgcc tcgactcgct
      541 ccgcggcctt ctcctggcgc agggaaaagc gcagcatcat cgccgccgac aggatggcgg
      601 cgagcggatt ggcgatgccc tttccggcga tatcgggcgc tgagccatgg atcggctcgt
      661 agagccccct gccgcgcgca tcgagcgccg ccgagggcag catgccgatc gatccggtga
      721 gcatcgatgc ctcgtccgac aggatgtcgc cgaacaggtt gccggtaacg atgacgtcga
      781 actgcttcgg attgcgcacc agttgcatcg cgcaattgtc gacgtacatg tgcgagagct
      841 cgacgtcgcc gtagtttttt gattcctgaa tgaccacttc gcgccagagc tgagaggttt
      901 cgagcacgtt cgccttgtcc accgagcaaa cgcgcctggc gcgcttgcgg gctgcctcga
      961 acgccacctt tgcgatcctg cggatctccg gctcgctgta gcgcatggtg tcgaagccct
     1021 cgcgcgcgcc gttcacctcg cggatgccgc gcggcgcgcc gaagtagatg tcgccggtga
     1081 gctcgcgcac gatcaggatg tcgagcccgc agaccacctc gaccttgagc ggcgacgccg
     1141 cagcaagctc gggatgcagg cgcgccgggc gcaggttggc gaacagaccc aattccttgc
     1201 gcagcccgag gatcgcctgc tcaggacgct tggcgcgcgg caagctgtcg tagcgcgggt
     1261 cgccgacagc gccgaacagg atcgcatccg cgtcttttgc gattttcaag gtcgcagggg
     1321 gaagcggatc gcccgccgac tcgtagccgg cgccgccgac cggcgcctgc tgcatttcca
     1381 gcccgaggtt caagcgctgc aggaccttgg tcgcctgcgc aatgatctcc ggcccgatgc
     1441 cgtcaccggg cagaacggcg accctcatct cagaaatacc agggaaactg cgctttgcgg
     1501 cgcgcttcga aggcgcggat ctcgtcggcg tggcgcagcg tgagcccgat ctcgtcgagc
     1561 ccattcagca ggcagtactt gcggaacggg tcgacctcga attgcatggt gcgcgaagca
     1621 tcgacttggg aaacggactg gttctccaaa ttcacaatca atctgaagcc tggaaaagcc
     1681 acgcactcgt agaacagctg gtcgacctcg gcctcggtca gcgccaccgg cagcagcccg
     1741 ttcttgaagc agttgttgaa gaagatgtcg gcgaacgacg gcgcgacgat gcagcgaaag
     1801 ccccactcca tcagcgccca gggcgcgtgc tcgcgcgacg agccgcagcc gaagttcttg
     1861 cgcgcaagca ggatcgccgc gccccggtag cgcggctggt tgagcacgaa ctccgggtcc
     1921 ttgcgccagg cgccgaacag cgcctcggag aagccggtgc gcttgatcga tttcaggtgc
     1981 tccttggcga tgatctggtc ggtgtcgacg ttggcgcggt cgagcggcgc caccaggcca
     2041 ttgaggacgg tgaatttttc catcgctcta gccccagcgg cggatgtcga cgaagtgtcc
     2101 cgcgatggcg gctgcggccg ccatcgcggg gctgaccagg tgcgtgcgcc cgccttcgcc
     2161 ctgccggcct tcgaagttgc ggttcgaagt cgaggcgcag cgctcgccgc tgccgaggcg
     2221 gtcggggttc atgccgaggc acatcgagca gcccggctcg cgccattcga agccggcgtc
     2281 acggaatatg cggtcgagcc cttcacgctc ggcttgggac ttcaccaccc cggagcccgg
     2341 caccaccagg gcgagccgca cgttgggggc gatgcgccgg cctttcacca ccgcggccgc
     2401 cgcgcgcagg tcctcgatgc gcgagttggt gcaagagccg atgaatatct tgtcgagctt
     2461 gatgtcggtg atgcgcgtac gcggcttgag gcccatgtag acgagcgcgc gctccatgcc
     2521 ttcacgccgc accgcgtcct tctcccggtc cggatcgggc accacgtcgt cgaccgacac
     2581 caccatctcg ggcgaggttc cccaagtgac gtgcggcttg atctcgcgcg cgtcgagcat
     2641 gacctcgcgg tcgaacttcg cgtcgggatc gcttttgagc gttcgccagt aggccacggc
     2701 gcgctcccag tctgcgccct tgggcgcaaa cggccgtcct ttgacatacg cgaccgtggt
     2761 ctggtcgcag gccaccatgc cggcgcgcgc gccggcctcg atcgacatgt tgcagaccgt
     2821 catgcgccct tccatcgaga gcgccctgat cgtgctgccg gcgaactcga tcgcatggcc
     2881 ggtgccgccc gcggtgccga tgcgcccgat gatggcgagc accacgtcct tcgcggtcac
     2941 gccgctcgcg agcggcccct cgacgctcac gcgcatgttc ctcatctttt gcgtcacgag
     3001 gcattgcgtg gcgagcacgt gttcgacctc cgaggtgccg atgccgtgcg cgaggcaggc
     3061 aaaggcgcca tgcgtgctgg tatgcgagtc gccgcacacc acggtcatgc ccggcagcgt
     3121 cgcgccctgc tccgggccga tgacgtgcac gatgccctgg cgcgcatcga gaaacggaaa
     3181 atagaccttg gcgccgaact cgcgcatgtt ggcgtcgagc gtctggatct gcagcttggc
     3241 gaacgggtcg cgcacgccct gcatgccctg gttccagtcg gtggtcgggg tgttgtggtc
     3301 ggcggtggcc accaccgagt cgatccgcca gggcttgcga cccgccacct tgaggccctc
     3361 gaaggcctgt gggctggtca cttcatgcac cagatgccgg tcgatgtaga gcagcgccgt
     3421 cccgtcgtgc ccgaggtgga cgaggtggct gtcccaaagc ttgtcgtaaa gcgtctgtcc
     3481 catgtttccg cgacttacgg agccaagcat tatctcagag accagctcgt ttcagccggt
     3541 gcgcaaaata cgcccctgcc agcgcggcga gcgccgacac gctgaatgcg gggccgggtc
     3601 cagaggcttc ccaagcccat ccggcaagca gggcgccagc cgcgccgccg gcgccgtagg
     3661 acaggctcga gaacagggtc tgtccgcgcg cctgggccgc gtgcggaaaa acgcggtgca
     3721 cggcggcgac cgaggcggca tggaaggcgc cgaagctggc agcgtgcagc agttgcgctg
     3781 cgaccaggat ccagagctcg gcggccgccc aac
//
LOCUS       DASPXW010000432         2122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1764357, whole genome shotgun sequence.
ACCESSION   DASPXW010000432 DASPXW010000000
VERSION     DASPXW010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1764357"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VEL04_09895"
     CDS             <1..386
                     /locus_tag="VEL04_09895"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYT48719.1"
                     /translation="FRISRLVAQSMVRRGRGHIIVITSIAAREVYNFGLIYCATKHAL
                     SAMARGLRIELQSQGIKVTEIAPGMVDTGIRATSDHPRVLEAFKARKFSPLTSEEVAE
                     AVLYAAQAAPNCCPDLIELRPRGSA"
     gene            408..818
                     /locus_tag="VEL04_09900"
     CDS             408..818
                     /locus_tag="VEL04_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYT48720.1"
                     /translation="MPSKTAIKSKPLQSFHVKHERAEDFDAGLRSYAKYRDLDLAKAT
                     AGMVQAHVIRMVPPCDPKVVSKWHYHDVDLQFVYVLKGWIKNEFEGQGVHTMKKGTCW
                     LQPPKIRHRVLDYSADCELLEVVLPAEFDTVEME"
     gene            841..2058
                     /locus_tag="VEL04_09905"
     CDS             841..2058
                     /locus_tag="VEL04_09905"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYT48721.1"
                     /translation="MSQERNTRRRILKGALAASALTAVPFISARAQSKAIKIGIPTVV
                     TGGYALLGSQVMRTCKLVQKMVSAKGGVIGRPVEFLFQDTAGDPAAAVRKSQDLVERD
                     DCRILTGVIVSSEAAAILPKLEQWNAVFISHGNGDGRLTAELFVPRFFRANTSAPMGA
                     RALALYLKDAPQKRFMAIASDAAWGRSSNKAFEEQIKRVGKSLVDQVFAPVANKDYSP
                     YITKIMQSGAEGCYVALQGDEARAFYSQAQQYKLPQRVQFYTEIVAQADIKVLGKDAI
                     GLMGSSRYPVTYDIPQNKAFLEAFRKEHGAELPDWTDGEMYQALMILFAGIEKAGSPE
                     PMKIVAAMEDLEVTSVKGPILMRKCDHQGENQGFVVKVAKNDKFAEPIAEIVKIYPRE
                     INTPDCRSASFSK"
BASE COUNT          401 a          693 c          682 g          346 t
ORIGIN      
        1 cgttccgcat ctcgcgcctg gtggcccagt ccatggtgcg gcgggggcgc ggccacatca
       61 tcgtcatcac ctcgatcgcc gcgcgcgagg tttacaactt cggcctcatc tactgtgcga
      121 ccaagcacgc gctttccgcc atggcgcgcg gcctgcgcat cgagctgcag tcgcagggca
      181 tcaaggtgac cgagattgcg cccggcatgg tcgacaccgg catccgcgcc acgagcgacc
      241 atccgcgcgt gctcgaggcg ttcaaggccc gcaagttttc cccgctcacc tccgaggaag
      301 tggccgaggc ggtgctctac gcggcgcagg cggcgccgaa ctgctgtccc gacctcatcg
      361 agctgcgccc gcgcggctcg gcctgaaccc atcatcgagg aactgtcatg cccagcaaaa
      421 ccgccatcaa atccaaaccg ctgcagagct tccatgtgaa gcatgagcgc gcagaggact
      481 tcgacgccgg gttgcgcagc tacgccaagt accgcgatct cgatctcgcc aaggcgaccg
      541 caggcatggt gcaggcgcac gtcatccgca tggtgccgcc ctgcgatccc aaggtggtct
      601 cgaaatggca ttaccacgac gtggatctgc agttcgtgta cgtgctgaag ggctggatca
      661 agaacgagtt cgagggccag ggcgtgcaca ccatgaagaa gggcacctgc tggctgcagc
      721 cgccgaagat ccgccaccgg gtgctcgact attcggcgga ctgcgagctg ctcgaggtgg
      781 tcctgccggc cgagttcgat accgtcgaga tggaatgact ttcccgagga ggagactgca
      841 atgagccaag agagaaacac acgccgccgc atcctgaagg gcgcgctcgc cgcttcggcg
      901 ttgaccgcgg tgccgttcat ctcggcacgc gcgcagagca aggccatcaa gatcggcatc
      961 cccacggtgg ttaccggcgg ctacgcgctg ctcggcagcc aggtgatgcg cacctgcaag
     1021 ctggtgcaga agatggtgag cgccaagggc ggcgtgatcg gccggccggt ggagttcctg
     1081 ttccaggaca cggcgggcga tcccgccgcc gccgtgcgca agtcgcagga tctggtcgag
     1141 cgcgacgact gccgcattct caccggcgta atcgtgtctt cggaggcggc cgcgatactg
     1201 ccgaagctcg agcagtggaa cgcggtgttc atctcgcacg gcaacggcga cggccgcctg
     1261 acggccgagc tcttcgtgcc gcgcttcttc cgcgccaata cctcggcgcc gatgggggcg
     1321 cgcgcgctcg cgctttacct caaggacgcg ccgcagaaga ggttcatggc gatcgcctcc
     1381 gacgccgcct ggggccgctc gagcaacaag gctttcgagg agcagatcaa gcgcgtcggc
     1441 aagagcctcg tcgaccaggt gttcgcgccg gtcgccaaca aggattattc gccctacatc
     1501 accaagatca tgcagtcggg cgccgagggc tgctacgtgg cgctgcaggg cgacgaagcg
     1561 cgcgccttct attcgcaggc gcagcagtac aagcttcccc agcgcgtgca gttctacacc
     1621 gagatcgtgg cgcaggcgga catcaaggtg ctcggcaagg acgcgatcgg cctgatgggc
     1681 tcttcgcgct acccggtcac ctacgacatc ccgcagaaca aggccttcct ggaagcgttc
     1741 cgcaaggagc acggagccga gctgcccgac tggaccgacg gcgagatgta tcaggcgctg
     1801 atgatcctgt tcgccggcat cgagaaggca ggctcgcccg agccgatgaa gatcgtcgcc
     1861 gcaatggagg acctcgaggt caccagcgtc aagggaccga tcctcatgcg caagtgcgat
     1921 caccagggcg aaaaccaggg ctttgtcgtt aaagtcgcga agaacgacaa atttgccgag
     1981 ccgatcgcgg agatcgtcaa gatctatccg cgcgagatca acacgccgga ctgccggagc
     2041 gccagcttca gcaagtgagc tttctcggct tcatcctggc gcagtgcctg agcgcactct
     2101 cgcaggcggc gatcctgttc tt
//
LOCUS       DASPXW010000433         9952 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_1890280, whole genome shotgun sequence.
ACCESSION   DASPXW010000433 DASPXW010000000
VERSION     DASPXW010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9952
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1890280"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /gene="priA"
                     /locus_tag="VEL04_09910"
     CDS             <1..895
                     /gene="priA"
                     /locus_tag="VEL04_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HYT48722.1"
                     /translation="CGWAAGCARCTARLVLHAAGAAWKTRNGSLRCHHCGAEQAVPRA
                     CPTCGNVDLKPMGRGTQRVEETLERCFPAARIVRIDRDSARRRAELSRTLEGIQRGEA
                     DILVGTQLLAKGHDFPGLTCVAVLNADTALLSTDYRAPERLFAVLAQVAGRAGRRSRP
                     GEVLVQTRYPEHPLFAALARHDFAGFADSQLEERRAAGFPPFVFEAALRAEAPKLERA
                     LAFLRAAASALEPPAEVHVYDPVPHVITRRAGFERAQLVMQSSSRPALQDYLSHLSQR
                     LFADSPRGVRWHLDVDPIEFD"
     gene            895..2544
                     /gene="argS"
                     /locus_tag="VEL04_09915"
     CDS             895..2544
                     /gene="argS"
                     /locus_tag="VEL04_09915"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HYT48723.1"
                     /translation="MLNPKDVFKSILEEGVRKVAPGAAVDVHIERPRNPEHGDLSTNI
                     ALQLSKQLGRKSREIAQQLVDATVPALLRSGITTPAGVSIAGPGFFNVRLNPAFKLQP
                     VHQVLKQGRDYGRAKSTSPEKIQVEFVSANPTGPLHVGHGRQAALGDAIATLLEWQGH
                     SVTREFYYNDAGAQIDKLALSVQARARGLKPGAPGWPEEGYNGEYVEEIARDFVVAKN
                     SVENLDAIRRFAVACLRKEQDADLQAFGVRFDVYCLESSLYTEGKVEQVVKALVASGK
                     TYEKDGALWLRTTDYGDDKDRVVRKSDGSYTYFVPDVAYHLGKWQRGFRKVINVQGTD
                     HHSTITRVRAGLQALGIGIPPGYPDYVLHSLVKVMRGGEEVKISKRAGSYVTVRDLID
                     WVGRDAVRFFLVSRKADSEFVFDVDLALEKTDENPVYYVQYAHARVCSVFSQSMSSGI
                     RGLKEAALDPLKTEKEMALVQRLGEFPEVVTEAAREAAPHSVAFYLRELAAEFHSYYN
                     AERILVEDEALRAARLALCAAVRQTLANGLSLLGVSAPEKM"
     gene            2544..3137
                     /locus_tag="VEL04_09920"
     CDS             2544..3137
                     /locus_tag="VEL04_09920"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HYT48724.1"
                     /translation="MQRTRTVSATRKRAAGGFLFGLFTGVAIGLAVSLAIAFYLNRTP
                     IPFVTAKKQAEKDAGAKAPAIAGLPQSAAASVAAPEKPKFDFYKILPGAEEPVTEREL
                     RERMRTARSQQDAPKDVYFIQAGSFQNPADADNQKARLAILGFESSVEPANLPDKGTW
                     YRVRMGPYTRIEEINRVRQQLAQNGIDASLVKIKEPH"
     gene            3146..3760
                     /locus_tag="VEL04_09925"
     CDS             3146..3760
                     /locus_tag="VEL04_09925"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HYT48725.1"
                     /translation="MKRFLLALLAACLAAGPALAQPQFAYTELKPAQPVETQGKIEVI
                     EFFWYGCPHCYNLEPFIESWLKKLPPDAQFRRVPAVFNERWGHDAAIYYTLEALGVID
                     KLHRPFFDAIHRDHLRTDSLPALNEWLEKNGIEPRRFNDTMKSFGVQSKLRRATQLTL
                     SYRIDGTPAMAVQGRYTVSADQGRSQTGMLDTVDYLVDMVRKKK"
     gene            3757..4494
                     /locus_tag="VEL04_09930"
     CDS             3757..4494
                     /locus_tag="VEL04_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYT48726.1"
                     /translation="MRIVLTGASSGIGEALARHYASPDTVLGLIARRPVSLSLPGTIT
                     RYQVDVTDEQALAATAQDFIARHGPPDLVIANAGVSVATCGEDIRDIASLRRVLEVNV
                     TGLAATLAAFAPAMRTAGRGTLAGIASVAGFRGLAGNGAYSASKSAAITWLESLRTEM
                     HGSGVAVVCICPGYIDTPLTRVNRFRMPFLLPPDEAARRIARAIARRPRLAVVPWQMA
                     LVSVALRAMPGWLFDRLAARAPRKPRT"
     gene            complement(4484..6298)
                     /gene="phaC"
                     /locus_tag="VEL04_09935"
     CDS             complement(4484..6298)
                     /gene="phaC"
                     /locus_tag="VEL04_09935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HYT48727.1"
                     /translation="MQPADPGESAELAKVCQSVAERSSRILGEFAQKQAEGLSSAVRD
                     EMGIAKAFMDLYARMAADPSVMATLSLSLWGDYLRLWQSSWMKLFGVQTQPVAEPANG
                     DYRFKDEDWSANFLFDVIKQSYLISARHIQQAVSSIAGLPEESEKRVAFFTRQYVDAL
                     SPSNFLLTNPQVLRETLASGGQNLVRGLNNLLADIEKGGGQLRISMTDESAFKLGQNV
                     ATTPGKVVYQNDLMQLIQYQPSTAEVFKRPLVIIPPWINKYYILDLREKNSFIRWALE
                     QGHTVFVISWVNPDARLAGKGFDDYMLEGPLAALDAVEKATGEREVNFIGYCLGGTLL
                     GATLGYLENKKENRVKSATFFVSLLDFSQPGELGVFIDEAQVASLERKMNQRGYLEGS
                     EMASTFNLLRANDLVWSFVVNNYLLGKDPFPFDLLYWNADSTRMPARMHSFYLRNMYI
                     KNLMGQPGGITLAGVPIDLSKVRLPCYFVSTVEDHIAPWKTAYRGARYLGAAVRGAVR
                     FVLGGSGHIAGIVNPPAAKKYHYWTSDSLPEIPEAWFEGATQHPGSWWEDWQAWIARL
                     NADAPKVAARVPGEGDLKGLEDAPGSYAMTRLPTKLKS"
     gene            complement(6285..7304)
                     /locus_tag="VEL04_09940"
     CDS             complement(6285..7304)
                     /locus_tag="VEL04_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYT48728.1"
                     /translation="MSSLSSSLEYPYPGTPAPGATLEVAPGVRWLSMPLPFQLDHINL
                     WLIGDTIVDTGIGDERTRALWEEVFKASDSQVRRVILTHYHPDHAGNAAWLCSRFGAE
                     LWTTQGEYLTAHAVRSSSAGYTTEAVLNVFRKNGLDAERSAGMAGRGNRYAELVPEFP
                     LSYRRIIEGDRVKIGAHEWRAFIGHGHAPEHLSLYCEALNVVIAGDMLLSTISTNVSV
                     WSIDPEGDPLRLFLESIARYRELPAGVLVLPSHGKPFRGAHARVAQLEAHHQDRLQEL
                     GKALREKPRSAGDLLGVLFRRPLDAHQTFFAMGEAIAHLHYLYYAGAAKRASGADGIM
                     RYAAS"
     gene            7331..7723
                     /locus_tag="VEL04_09945"
     CDS             7331..7723
                     /locus_tag="VEL04_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HYT48729.1"
                     /translation="MQIEETERTEFSISDLAREFDVTPRAIRFYEDQGLISPRREGQR
                     RVYTPRDKTRLKLTLRGKRLGLSLSEIKELIDMYEPGRDERPQLERFLAVLESHKSGL
                     LQQRADIEAQLSEIHTFEKRVRKQLKRG"
     gene            7782..8195
                     /locus_tag="VEL04_09950"
     CDS             7782..8195
                     /locus_tag="VEL04_09950"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HYT48730.1"
                     /translation="MNYIRESFARQKAMALIGASLTGVSAGKTEVSLPYRDDLTQQKG
                     FVHGGIIGMIADTACGYAAYSLMPASASLVTVEYKINILAPARGALVARGEVVKAGRT
                     LTVARAEVYAQDGTHIATMQQTLMMLPDTADTPDP"
     gene            8195..9331
                     /locus_tag="VEL04_09955"
     CDS             8195..9331
                     /locus_tag="VEL04_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HYT48731.1"
                     /translation="MFLNFDHGETIAMLRDSVRDFAAKEIAPRAGEIDRSNEFPPDLW
                     PKLGALGLLGVTVEEEYGGTLMGYLAHIIAMEEISRASASIGLSYGAHSNLCVNQIRR
                     NGAAAQKKKYLPKLVSGEHVGALAMSEPGAGSDVVGMKLKAERRGDRYVLNGNKMWIT
                     NGPDADVLVIYGKAEGITAFIVEKGMKGFSTAQKLDKLGMRGSNTCELVFRDCEVPVE
                     NVLGEEGHGVKVLMSGLDYERAVLAGGPLGIMAACLDVVMPYVHERKQFGQPIGEFQL
                     MQGKLADMYTTFSACRAYVYAVGQALDRGETTRKDAAGAILYAAEKATWMAGEAIQAL
                     GGMGYINETPTGRLWRDAKLYEIGAGTSEIRRWLIGRELFAESQ"
     gene            9328..>9952
                     /locus_tag="VEL04_09960"
     CDS             9328..>9952
                     /locus_tag="VEL04_09960"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HYT48732.1"
                     /translation="MKLKNSFTEKLGLRLPLVAAPMAGGPTTPELVAACSSAGALGSF
                     GFAYTQPEEMKKQAAAVRSKTERPFGINLFVAPQPEPIDPAAQKNALQAVARYYQELH
                     LPAPEPVRAPYAPDLEAQLAAVEEIRPRVFTVHLGALSAEKIKRFRNLNIIVGGSATC
                     VAEAQRLESLGFDFVIAQGGEAGGHRGSYLRDPLEALTGTLALTRLVV"
BASE COUNT         1765 a         3277 c         3250 g         1660 t
ORIGIN      
        1 ctgcggctgg gcggcgggct gcgcgcgctg caccgcgcgc ctggtgctgc acgcggccgg
       61 ggccgcctgg aaaactcgca atggtagcct gcgctgccac cattgcggcg ccgagcaggc
      121 cgtgccgcgc gcctgtccga cctgcggcaa cgtcgacctc aagcccatgg gccgcggcac
      181 gcaacgcgtc gaggagacgc tcgagcggtg ctttcccgcg gcgcgcatcg tgcgcatcga
      241 ccgcgacagc gcgcggcggc gcgccgagct ctcgcgcacg ctcgaaggca tccagcgcgg
      301 cgaagccgac atcctcgtcg gcacgcagct gctcgccaag ggccacgact tcccgggtct
      361 cacctgcgtc gcggtgctga atgccgacac cgcgctgctc tcgaccgatt accgcgcgcc
      421 ggagcggctg ttcgcggtgc tcgcgcaggt cgccgggcgc gcgggtcggc gcagccgtcc
      481 gggcgaagtg ctggtgcaga cccgttaccc ggagcacccg ctgttcgccg cgctcgccag
      541 gcacgacttt gcgggattcg ccgattcgca gctcgaagag cgccgcgccg ccggctttcc
      601 gccgttcgtg ttcgaagcgg cactgcgcgc cgaagcgccc aagctcgagc gcgcgctcgc
      661 cttcctgcgc gccgccgcct cggctctgga gccccctgcc gaagtgcacg tctacgaccc
      721 cgtgccgcac gtcatcaccc gccgcgccgg cttcgagcgc gcgcagctgg tgatgcaatc
      781 gtcctcgcgc ccggctctgc aggactattt gtcgcacttg agccaaaggc tgttcgccga
      841 ttccccgcgc ggggtgcgct ggcacctcga tgtcgatccc atagaattcg actgatgctc
      901 aatccgaaag acgtgttcaa gagcattctc gaggagggcg tgcgcaaggt ggcccctggc
      961 gccgcagtcg atgtgcacat cgagcggccc aggaatccgg agcacggtga tctgtcgacc
     1021 aacattgcac tgcagttatc gaagcaactt gggcgcaagt cgcgcgaaat tgcgcagcag
     1081 cttgtcgatg ccaccgtgcc tgctttgctt agaagcggca ttacaactcc ggcaggagtt
     1141 tcgatcgcgg ggccgggctt tttcaatgtt cgtctgaacc ccgctttcaa gctgcaaccc
     1201 gtccaccagg ttctcaaaca gggacgcgac tacggccgcg ccaaatcgac ctcgccggag
     1261 aagattcagg tggagttcgt gtccgcgaac ccgaccggac ccttgcacgt cggccatggc
     1321 cgccaggcgg cgctcggcga tgcgatcgcg accttgctgg agtggcaggg ccattcggtc
     1381 acgcgcgagt tttactacaa cgacgccggc gcccagatcg acaagctcgc gctgtccgtg
     1441 caggcgcgtg ccagggggtt gaagccaggc gctcccggct ggcccgaaga aggctacaac
     1501 ggcgaatacg tcgaagaaat cgcccgcgat tttgttgtgg cgaaaaacag cgtcgaaaac
     1561 ctggacgcga tacgccgctt cgccgtggcg tgcctgcgca aagagcagga cgccgatctg
     1621 caggctttcg gcgttcgatt cgacgtctac tgcctcgaat ccagcctcta caccgaaggc
     1681 aaggtcgagc aggtggtcaa ggcgctggtc gcgagcggca agacctacga gaaggacggc
     1741 gcgctctggc tgcgcaccac cgactacggc gacgacaagg accgggtggt gcgcaaatcg
     1801 gacggcagct acacctactt cgtgccggac gtcgcctatc acctcggcaa gtggcagcgc
     1861 ggcttccgca aggtcatcaa cgtgcagggc accgatcacc acagcacgat cacgcgcgtg
     1921 cgcgccgggc tgcaggcgct cggcatcggc atcccgccgg gctatcccga ctacgtgctg
     1981 cacagcctgg tgaaggtgat gcgcggcggc gaggaagtga agatttccaa acgtgcaggc
     2041 tcgtacgtca ccgtgcgcga cctgatcgac tgggtgggcc gcgacgcggt gcgctttttc
     2101 ctggtctcgc gcaaggccga ttccgagttc gtgttcgacg tcgacctggc gctcgaaaag
     2161 accgacgaga acccggtgta ttacgtgcag tacgcgcacg cgcgtgtctg cagcgtgttt
     2221 tcgcaatcaa tgtcttcggg gatccggggg ctgaaggaag cagcgcttga tcctctgaaa
     2281 accgaaaaag aaatggcgct ggtgcagcgg ctcggcgagt ttcctgaggt cgtcaccgag
     2341 gcggcgcgcg aggccgcgcc gcattcggtc gcgttctacc tgcgcgaact cgcggctgag
     2401 ttccacagct actataatgc cgagcgcatc ctcgtcgaag acgaggccct gcgcgcggcg
     2461 cgtcttgcgc tgtgtgcggc agtgcgccaa acgctcgcca acgggctgtc gctcctaggc
     2521 gtcagcgctc cggagaagat gtgatgcaaa gaacgaggac cgtttccgcc acgcgcaagc
     2581 gcgcggcagg cggcttcctg ttcggcctgt tcaccggcgt ggccatcggc ctggccgtgt
     2641 cgctcgccat cgccttctac ctcaaccgca cgccgattcc cttcgttacc gcgaagaagc
     2701 aggcggagaa ggacgcgggc gcgaaggcgc cggcgatcgc cggcctgccg cagagcgccg
     2761 cggcgtcggt cgccgcgccc gagaagccga aattcgactt ctacaagatc ctgcccggcg
     2821 cagaggagcc ggtcaccgag cgcgagctgc gcgagcgcat gcgcacggcg cgcagtcaac
     2881 aggatgcgcc gaaggacgtt tatttcatcc aggcgggctc gttccagaac ccggccgacg
     2941 ccgacaacca gaaggcgcgg ctcgcgatcc tgggattcga gtcgagcgtc gagccggcga
     3001 acctgcctga caagggcacc tggtaccgcg tgcgcatggg accgtacacc cggatcgagg
     3061 agatcaaccg ggtgcgccag cagctggcgc agaacggcat cgacgcgagc ctggtgaaga
     3121 tcaaggagcc gcactaggaa agcacatgaa acgatttctt ctagctttgc tcgcagcatg
     3181 cctcgccgca ggcccggcgc tcgcgcaacc gcaattcgcc tatacggagc tgaagcccgc
     3241 tcagccggtc gagacccagg gcaagatcga ggtgatcgag ttcttctggt acggctgccc
     3301 gcactgctac aacctggagc cgttcatcga gagctggctc aagaagctgc cgcccgacgc
     3361 gcagttccgg cgcgtgccgg cggtgttcaa cgagcgctgg ggacatgacg cggcgatcta
     3421 ctacaccctc gaggcgctcg gcgtgatcga caagctgcac cgcccgtttt tcgacgccat
     3481 ccaccgcgac cacctgcgca ccgacagcct cccggcgctc aacgagtggc tcgagaagaa
     3541 tggcatcgag ccgcgcaggt tcaacgacac gatgaagtca ttcggggtgc agagcaagct
     3601 gcgccgcgcc acgcagctca ccctgtcgta caggatcgac ggcacgccgg ccatggcggt
     3661 gcaggggcgc tataccgtga gcgcggacca gggccgctcg caaacgggca tgctcgacac
     3721 ggtcgactac ctggtcgaca tggtcaggaa gaagaaataa gaatcgtcct cacaggcgct
     3781 tcctcgggca tcggcgaagc gcttgcgcgg cactacgcct cgccggacac ggttctgggc
     3841 ttgattgccc ggcgaccggt cagcctgagc ctgcccggca ccatcacccg ctatcaagtc
     3901 gacgtgaccg acgagcaggc gctcgccgcc actgcccagg atttcatcgc gcgccacggc
     3961 ccgccggatc tggtcatcgc gaacgccggc gtgtccgtcg cgacctgcgg tgaggacatt
     4021 cgcgacatag cgagcttgcg gcgcgtgctc gaagtcaacg tcacagggct tgccgcaacc
     4081 ctcgctgcgt ttgcgcccgc gatgcgcacg gcgggccgcg gcacgcttgc cggcattgcc
     4141 agcgtcgcgg gcttccgcgg gctggcgggc aatggcgcct acagcgcctc gaaatcggcc
     4201 gctatcacct ggctggagag cctgcgcacc gaaatgcatg gcagcggcgt cgcggtggtc
     4261 tgcatctgcc ccggctacat cgataccccg ctgacgcgcg tcaaccgctt tcgcatgccg
     4321 tttcttctgc ctcccgacga ggcagcacgc cgcatcgcgc gtgccatcgc ccggcgcccg
     4381 cgcctcgcgg tcgttccctg gcagatggcg ctggtatcgg tagcgctgcg tgcaatgcca
     4441 ggctggcttt tcgaccgcct ggcggcgcgg gcgccgcgca agcctaggac ttgagcttcg
     4501 taggcaggcg cgtcatggcg tagctgccgg gcgcgtcctc cagccccttc aggtccccct
     4561 ctcccggcac gcgcgccgcg actttcgggg cgtcggcgtt cagccgcgcg atccatgcct
     4621 gccagtcttc ccaccacgag cccggatgct gggtcgcgcc ctcgaaccat gcctcgggaa
     4681 tctccggcag ggagtcgctg gtccagtagt ggtacttctt cgccgcaggc gggttgacga
     4741 tgccggcgat atggcccgag ccgccgagga caaagcgaac cgccccccgc accgctgcgc
     4801 cgaggtagcg cgcgccccgg taggccgtct tccacggcgc aatgtggtcc tcgacggtcg
     4861 agacgaagta gcacggcaac cgcacctttg aaaggtcgat cggcacgccg gcgagcgtga
     4921 taccgccggg ctggcccatc aggttcttga tgtacatgtt gcgcagatag aagctgtgca
     4981 tgcgcgccgg catgcgcgtc gaatcggcgt tccagtacag caggtcgaac ggaaacgggt
     5041 ccttgccgag aagatagttg ttcaccacga acgaccacac cagatcgttg gcgcgcagca
     5101 ggttgaaggt gctcgccatc tccgagcctt cgaggtagcc gcgctggttc atcttgcgct
     5161 cgaggcttgc cacctgcgcc tcgtcgatga acacgccgag ctcgccgggc tgcgagaagt
     5221 cgagcaggct gacgaagaac gtggcgcttt tgacgcgatt ttctttttta ttttcgaggt
     5281 aacccagcgt cgcgccaagg agcgtgcctc ccaggcaata gccgatgaaa ttcacctcgc
     5341 gctcgccggt ggccttctct accgcatcga gcgccgcgag cgggccttcg agcatgtagt
     5401 cgtcgaagcc cttgccggcg agccgcgcgt ccgggttcac ccacgagatg acgaataccg
     5461 tgtggccctg ctcgagcgcc cagcggatga acgagttctt ctcgcgcaga tcgaggatgt
     5521 agtacttgtt gatccacggc gggatgatga ccagcggccg cttgaatacc tcggcggtgc
     5581 tcggctggta ctggatgagc tgcatcaggt cgttctggta gacgaccttg cccggggtgg
     5641 tggcgacgtt ctgcccgagc ttgaacgcgc tctcgtcggt catcgagatg cgcagctgcc
     5701 cgccgccttt ctcgatgtcg gcgagcaggt tgttcagccc gcgcaccagg ttctggccgc
     5761 cgctcgcgag cgtctcgcgc agcacctgcg ggttggtgag cagaaagttc gatggcgaaa
     5821 gcgcatcaac gtactggcgg gtgaagaagg ccaccctctt ctccgactcc tcgggaagcc
     5881 ctgcgatgct cgacacggcc tgctggatgt ggcgcgccga gatcaggtag gactgcttga
     5941 tgacgtcgaa caggaagttc gcagaccagt cctcgtcctt gaagcggtag tcgccgttgg
     6001 ccggctcggc gaccggctgg gtctgcacac cgaagagctt catccagctc gattgccaga
     6061 gccgcaggta atcgccccac aggctcagcg aaagcgtggc catgacgctc gggtccgccg
     6121 ccatgcgcgc gtacaggtcc atgaacgcct tggcgatgcc catctcgtcg cgcacggcgg
     6181 aggagagccc ctcggcctgc ttctgggcga attcgccgag gatcctggag gagcgctcgg
     6241 cgaccgactg gcacactttg gcgagctctg cggactcgcc cgggtcagct ggctgcatag
     6301 cgcatgatgc catcagcacc gctcgcgcgc tttgcggctc ccgcgtagta caggtagtgc
     6361 aggtgggcta tcgcctcgcc catcgcgaag aaagtctggt gggcgtcgag cggacggcga
     6421 aacagcacgc cgaggagatc gcccgcgctg cggggtttct cccgcagtgc cttgccgagc
     6481 tcctgcagac gatcttggtg atgcgcctcg agctgcgcca cgcgcgcgtg agcgccgcgg
     6541 aacggcttgc catgcgaggg cagcaccagg acgcctgcgg gcagctcgcg gtagcgcgcg
     6601 atcgactcga gaaacaagcg caagggatcg ccctcggggt cgatcgacca cacgctgacg
     6661 ttggtcgaga tggtcgaaag cagcatatcg cccgcgatca ccacgttgag tgcctcgcag
     6721 tagagcgaga ggtgctccgg agcgtggccg tgcccgatga aggcgcgcca ttcgtgggcg
     6781 ccgatcttca cccggtcgcc ctcgatgatg cgccggtagg acaacgggaa ttcgggcacc
     6841 agctcggcgt agcggttgcc gcgtcccgcc atcccggcgg agcgctccgc gtcgaggccg
     6901 tttttgcgga agacattcag cacagcctcg gtggtgtagc ccgcgctcga ggagcgcacc
     6961 gcgtgcgccg tcaggtattc cccctgagtc gtccagagct cggcgccgaa tcggctgcag
     7021 agccaggccg cgttgcctgc gtggtcggga tggtagtgcg tcaggatgac tcgtcttacc
     7081 tgtgaatcgc ttgctttaaa aacttcttcc cacagggcgc gcgtgcgttc atcgccgatt
     7141 cccgtgtcga cgatcgtgtc gccgatcagc cacaggttga tatggtcgag ctggaacggc
     7201 agcggcatgc tgagccagcg cacgccaggg gcgacctcga gggtggcgcc gggtgccggg
     7261 gtgccggggt agggatactc cagggaagaa gacagggaag acattccgct agaatcagtc
     7321 gcgagccgcc atgcagatcg aggaaacgga gcgcaccgag ttttcgatca gcgacctggc
     7381 gcgcgagttc gacgtcacgc cgcgcgcgat ccgcttctac gaggaccagg gactcatctc
     7441 gccgcggcgc gaaggccagc gccgagtgta cacgccgcgc gacaagaccc ggctcaagct
     7501 caccctgcgc ggcaagcggc tcggcctctc cctctccgag atcaaggagc tgatcgacat
     7561 gtatgagccg gggcgcgacg agcgaccgca gctcgagcgc ttcctcgccg tgctcgaatc
     7621 gcacaagtcg ggcctgctgc agcagcgcgc cgacatcgaa gcccagctat ccgagatcca
     7681 tactttcgag aaacgcgtaa gaaagcagct gaaacgcggt taagcgccta tcttacgcaa
     7741 ggttgacgtt tacgtaaacg tcaagtagtc tccctaagct catgaattac atacgggaaa
     7801 gctttgcccg ccagaaggcg atggccctca tcggggcatc gctcacaggg gtgtctgcag
     7861 gaaagaccga agtcagcctg ccgtaccgcg acgatctgac gcaacagaag ggcttcgtgc
     7921 atggcggcat catcggcatg atcgccgaca cggcctgcgg ctatgccgcg tattcgctga
     7981 tgccggcgag cgcctcgctg gtcaccgtgg agtacaagat caacattctc gccccggcgc
     8041 gcggcgcgct ggtcgcgcgc ggcgaggtgg tcaaggccgg gcgcacgctt accgtggccc
     8101 gcgccgaggt gtacgctcag gacggcacgc atatcgcgac catgcagcag accttgatga
     8161 tgctgcccga taccgccgac acgccagacc cataatgttc ctgaacttcg atcacggcga
     8221 aaccatcgcc atgctgcgcg atagcgtgcg cgatttcgcg gcgaaggaga tcgcgccgcg
     8281 cgcgggcgag atcgaccgca gcaacgagtt tccgcccgac ctgtggccga agctcggcgc
     8341 gctcggcctg ctcggcgtca cggtcgagga ggagtacggc ggcaccctga tgggctatct
     8401 cgcccacatc atcgccatgg aagagatctc gcgcgcctcg gcctcgatcg ggctttccta
     8461 cggcgcgcac tccaatctgt gcgtcaacca gatccgccgc aacggcgccg cggcgcagaa
     8521 gaagaaatac ctgcctaagc tcgtgtccgg cgagcatgtc ggcgcgctcg ccatgtcgga
     8581 gcccggcgcc ggatcagatg ttgttggaat gaaattaaaa gctgaaaggc gtggggaccg
     8641 ctacgtcctg aacggcaaca agatgtggat taccaatggc cctgacgccg atgtgctggt
     8701 gatctacggc aaagccgaag ggattactgc tttcatcgtc gaaaaaggca tgaagggctt
     8761 ctcaactgcg cagaagctgg acaagctggg tatgcgcggc tccaacactt gcgagctggt
     8821 attccgcgat tgcgaagtgc ccgtcgagaa tgtcctcggc gaggaaggtc acggcgtgaa
     8881 ggtgctgatg tcgggcctcg attacgagcg cgccgtgctt gccggcgggc cgctcggcat
     8941 catggcggcc tgcctcgatg tcgtcatgcc ttacgtccac gagcgcaagc agttcggcca
     9001 gccgatcggg gaattccagc tgatgcaggg caagctcgcc gacatgtaca ccaccttctc
     9061 cgcctgccgc gcctacgtgt acgcggtagg acaggccctc gaccggggcg agaccaccag
     9121 aaaggacgcc gcaggagcga tcctctacgc cgccgagaag gccacctgga tggcgggcga
     9181 ggcgatccag gcgctcggcg gcatgggcta catcaacgag acgccgaccg gcaggctgtg
     9241 gcgcgacgcc aagctctacg agatcggcgc cggcacctcc gagatccgcc gctggctgat
     9301 cggccgcgag ctgtttgccg aatcgcaatg aaactgaaga attcctttac tgaaaaattg
     9361 ggcttgcgcc tgccgctggt cgcggcgccg atggcaggag gtccaaccac gccggaactg
     9421 gtcgcggcct gctcgtcagc cggtgcgctc ggctcgttcg gcttcgccta tacccagccc
     9481 gaagagatga aaaagcaggc cgccgcggtg cgctcgaaga ccgagcggcc gttcggcatc
     9541 aacctgttcg tcgcgccaca acccgagccg atcgaccccg cggcgcagaa gaacgcgctg
     9601 caggcggtcg cgcggtatta ccaggaattg catctgcccg ccccggaacc ggtacgcgcg
     9661 ccgtatgcac ccgacctcga agcccagctt gccgcggtcg aggaaatccg gccgcgcgtt
     9721 ttcacggtgc atctgggtgc tctttccgca gagaaaataa aacgcttcag gaacctgaac
     9781 atcatcgtgg gcggcagcgc gacctgcgtc gcggaggcgc agcgcctgga gtctctcggt
     9841 ttcgacttcg tcatcgcgca gggcggcgag gccggcgggc accgcggcag ctatttgcgc
     9901 gatccgctcg aggcgctcac cggcactctc gccctgaccc gcctggtagt gc
//
LOCUS       DASPXW010000434         2961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11577
            k141_503959, whole genome shotgun sequence.
ACCESSION   DASPXW010000434 DASPXW010000000
VERSION     DASPXW010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945307
            Sequence Read Archive: SRR6266545
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:20:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,998
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503959"
                     /isolate="SMAG_U11577"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VEL04_09965"
     CDS             <1..299
                     /locus_tag="VEL04_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HYT48733.1"
                     /translation="KKGDFVFRYTEPDDFGAPQNAFLVCSFWFVDALAATGRREEARA
                     LFQSLLACRNRHGLLAEHVDPSTRELWGNFPQTYSMVGLINSATRLSLPWEQAF"
     gene            299..1141
                     /locus_tag="VEL04_09970"
     CDS             299..1141
                     /locus_tag="VEL04_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HYT48734.1"
                     /translation="MRLGVDVGGSKIEVAVLDGEGRVALRQRAVTPHGSYEEALQALC
                     AMVLSAERDAGEPCNVGVGIPGALSPRSGLVQNAYNTPLNGRPLKRDLEAVLERGVRL
                     ANDANCFALSETLDGAARGARVVFGAILGTGAGGGVAVDRRILEGRNAIGGEWGHNPL
                     PWMTRDEHPGPACYCGRSGCIEQFVSGPALARDKTAVGESAALARYQDRLARALAHVV
                     NLLDPDVIVLGGGVSNLERLYAEVPPLLARYVYTHSFDTPLLRAAHGDASGVRGAAML
                     WDQA"
     gene            complement(1198..1404)
                     /locus_tag="VEL04_09975"
     CDS             complement(1198..1404)
                     /locus_tag="VEL04_09975"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYT48735.1"
                     /translation="MRDRSVQGQGLGELLLVDAITRTRSVLEHVGVHALFVDAKDREA
                     AGFYRRYGFRPLPSDPLRLVLPLA"
     gene            complement(1519..1866)
                     /locus_tag="VEL04_09980"
     CDS             complement(1519..1866)
                     /locus_tag="VEL04_09980"
                     /inference="COORDINATES: protein motif:HMM:NF020265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HYT48736.1"
                     /translation="MTLSNDERGRITARVPQNVQDTLQRAAELLGATLNQFVVQAALN
                     EARRVIERDRVIHLSSTDAAFLMNLLEKPPAPNARLRKAFQTYKKRTLDAEHSTFNWT
                     PRAKRVRERKRAA"
     gene            complement(1976..2482)
                     /locus_tag="VEL04_09985"
     CDS             complement(1976..2482)
                     /locus_tag="VEL04_09985"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYT48737.1"
                     /translation="MEPALKCLKFLSHGTLESRDLEKTRAFYEECLGIETVRTSPISL
                     MIRLGGDNTIAIVQNPKKPPMPLLNHNGLDVATRQEVDECHRVLEEMKQRWDIRKLTK
                     PADQHGTYSFYFSDCDENWWEILVNPAGGYSWMFQGHDLERWGAGKDESNPNDYRPTA
                     KKWVNPGG"
     gene            complement(2485..>2961)
                     /locus_tag="VEL04_09990"
     CDS             complement(2485..>2961)
                     /locus_tag="VEL04_09990"
                     /inference="COORDINATES: protein motif:HMM:NF017778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HYT48738.1"
                     /translation="ALARLARAARHQDFGELEETLHVAVRKPAWLRPEHRVGDPNGYM
                     RHLLYIDPQDAFVITAITWLPGQVSALHGHRVWCMYGVAEGELTEQQFSNSKNQLEIV
                     KEKLYPAGTLADSDLEQRMIHRVSNRGRSRVVSLHLYGVSAGRLTTGINRIYPEEA"
BASE COUNT          439 a          952 c         1006 g          564 t
ORIGIN      
        1 tgaaaaaggg cgacttcgta ttccgctaca ccgaaccgga cgacttcggc gcgccgcaga
       61 acgccttcct ggtgtgcagc ttctggttcg tcgatgcgct cgctgcgacc gggcggcgcg
      121 aggaggcgag ggcgctgttc cagagcttgc ttgcatgccg caaccggcac gggctgctcg
      181 ccgagcacgt cgatccgtcc acgcgcgagc tgtggggcaa tttcccgcag acctacagca
      241 tggtcgggct gatcaattcg gccacgcggc tttccctgcc gtgggagcag gcgttttgat
      301 gcgcctgggc gtcgacgtcg gcggcagcaa gatcgaagtg gcagtgctgg acggggaagg
      361 tcgcgtagcg ctgcggcagc gcgcggtgac gccgcacggc tcctacgagg aggcgctgca
      421 ggccctgtgc gcgatggttc tgtctgccga gcgcgatgcg ggcgagcctt gcaacgtggg
      481 cgtcggcatt cccggcgcgc tgtcgccgcg ctccggtctg gtgcagaacg cctacaacac
      541 gccgctcaac ggccggcctc tcaagcgcga cctcgaggcc gtcctggagc gcggggtgcg
      601 gctcgccaac gacgccaatt gcttcgctct ttccgaaaca ctggacggcg ccgcgcgcgg
      661 cgcgcgggtc gtgttcggcg ccatcctggg caccggcgcg ggcggcggcg tcgccgtgga
      721 ccggcgcatc ctcgaaggcc gcaatgcgat cggcggcgag tggggtcata accctctgcc
      781 ctggatgacg cgcgacgagc atcccgggcc ggcgtgctac tgcgggcgct cgggctgtat
      841 cgagcagttc gtatccgggc cggcgctggc gcgcgacaag accgcggtgg gcgagagcgc
      901 cgcgctggcg cgctaccagg accggctggc gcgcgccctc gcgcacgtcg tgaacctgct
      961 cgatccggac gtcatcgtgc tcggcggcgg agtgtccaac ctcgagcgcc tgtatgccga
     1021 ggtgccgccg ctgctcgcgc gctacgtgta cacgcacagc ttcgatactc cgctgctgcg
     1081 cgcggcgcac ggcgatgcga gcggcgtgcg cggagcggcg atgctctggg atcaggcgta
     1141 aacatggatg atcttctgct cgaggtcgac gccggcaagg gttccaggcg gtcgaaatca
     1201 ggctagcggc aggacgaggc gcagcggatc gctcggcagt ggccgaaacc cataccttcg
     1261 gtagaacccg gccgcctccc ggtccttcgc gtccacgaaa agcgcatgca cgcccacgtg
     1321 ttcgagcacg ctcctggtgc gtgtgatcgc gtccacgagc agcagctcgc ctagtccctg
     1381 gccctgcacc gagcggtctc gcacgcagtc agggcgtaga acccgaggat gcgcgaaggc
     1441 gcgtcgtccg cgaccgcgac gaacgttttt gagataccgc gtttctggtg ctgctgcgcg
     1501 atctggcgca gccaggcgtc aagctgcgcg cttccgctct cgaacgcgct tcgctcgtgg
     1561 cgtccagtta aaggttgaat gctcagcatc gagcgtcctc ttcttgtagg tctggaacgc
     1621 cttgcggagc cttgcattcg gcgccggcgg cttctccagc aaattcatga gaaacgccgc
     1681 gtcggtgctg gacagatgga tcacccggtc gcgctcgatc acccgccgcg cctcattcag
     1741 cgcggcctgc acgacaaact gattcaacgt cgcccccagc agttcagccg cccgctggag
     1801 ggtgtcctgc acgttctgcg gcaccctggc cgtgatccgg ccgcgttcgt cgttcgagag
     1861 tgtcattcgg gtttggtagc ggctggcagg tttgggagtt gtcagtttgc ggtttgaatg
     1921 agctgttggt gccaaattgt cacctccatc agatccgatc ctctctgatt cacccctagc
     1981 cgccgggatt gacccatttc ttggccgtgg gccggtagtc gttcgggttc gactcgtcct
     2041 tgccggcgcc ccagcgctcg aggtcgtgcc cctggaacat ccagctgtag ccccccgcgg
     2101 gattgaccag gatctcccac cagttctcgt cgcaatcgga gaagtagaac gagtaggtgc
     2161 cgtgctggtc cgcgggcttg gtgagcttcc tgatgtccca gcgctgcttc atttcctcca
     2221 gcacgcggtg gcactcgtcg acctcctggc gcgtcgcgac atcgaggccg ttgtggttga
     2281 ggagcggcat cggcggcttc ttcgggttct gcacgatcgc gatggtgttg tcgccgccga
     2341 gccggatcat gagcgagatc gggctggtgc gcacggtctc gatgccgagg cattcctcgt
     2401 agaaagcgcg cgtcttttcc aggtcgcgcg attccagcgt gccgtgcgag agaaacttca
     2461 ggcatttgag cgcgggttcc atggtcaggc ctcctcgggg tagatgcggt tgattccggt
     2521 ggtcagccgc ccggcgctga cgccgtagag atgcagcgat actactctcg aacgaccgcg
     2581 gttcgagacg cggtggatca tcctctgctc gaggtcgctg tcggcaaggg tgccggccgg
     2641 ataaagcttt tctttcacga tttcaagttg gtttttggag ttgctgaatt gctgctcggt
     2701 cagctcgcct tccgcgacgc cgtacatgca ccacacccgg tgcccgtgca gcgcgctcac
     2761 ctgcccgggc agccaggtga tcgcggtgat gacgaaagca tcttgcgggt cgatgtagag
     2821 caggtgccgc atgtagccgt tcgggtcgcc gacgcgatgc tcgggacgca gccaggcggg
     2881 cttgcgaact gcgacgtgca gcgtctcctc cagctcgccg aagtcctggt gtcttgcggc
     2941 gcgcgccagg cgcgcaagcg c
//
